Industrially Useful Microorganism

Abstract

According to the present invention, the E. coli mutant strain having chromosomal DNA that is at least 470 kbp shorter than that of a wild-type E. coli strain exhibits such a property that the number of the cells after a certain period of culture is greater than a wild-type strain. Thus, by culturing the mutant strain in a medium so as to generate and accumulate a useful substance in the culture, a useful substance such as proteins, peptides, amino acids, nucleic acids, vitamins, saccharides, organic acids, and lipids can be efficiently produced.

Description

TECHNICAL FIELD

The present invention relates to an E. coli mutant strain having chromosomal DNA shorter than that of a wild-type E. coli strain and a process for producing a useful substance using such mutant strain.

BACKGROUND ART

All nucleotide sequences of the chromosomal DNAs of E. coli have been elucidated (Non-Patent Documents 1 and 2). Also, a method wherein a given gene or a given region on chromosomal DNA of E. coli is deleted as designed by homologous recombination has been known (Non-Patent Document 3). With the utilization of all of the nucleotide sequence information of chromosomal DNA and homologous recombination, a library of mutant E. coli in which genes of chromosomal DNA are comprehensively disrupted, a library of mutant E. coli in which approximately 20-kbp deletable regions of chromosomal DNAs are comprehensively deleted, and the like have been constructed (Non-Patent Documents 4 and 5).

As example of E. coli mutants that lack multiple regions on chromosomal DNA, a mutant that lacks a 376-kbp region (Non-Patent Document 6), a mutant that lacks a 262-kbp region (Non-Patent Document 7), and a mutant that lacks a 313-kbp region (Non-Patent Document 5) have been reported. However, the growth (the amount of cells or the growth rate) of such mutant strains is merely described as equivalent to that of their parental wild-type strains, and the influence of the introduction of deletions that are 400 kbp or longer into chromosomal DNA imposed upon the growth of E. coli is not described.

That is, an E. coli mutant, which lacks multiple regions on chromosomal DNA and the growth of which is improved over that of a wild-type E. coli strain, and a process for producing a useful substance using such mutant have not yet been known.

• Non-Patent Document 1: Science, 277, 1453, 1997
• Non-Patent Document 2: DNA Data Bank of Japan, “Escherichia coli K12 W3110,” online, Oct. 29, 2004, Internet <URL: http://gib.genes.nig.ac.jp/single/index.php?spid=Ecol_K12_W3110>
• Non-Patent Document 3: J. Bacteriol., 180, 2063, 1998
• Non-Patent Document 4: Tanpakushitsu, kakusan, kouso (Protein, nucleic acid, enzyme), vol. 46, p.2386, 2001
• Non-Patent Document 5: Nature Biotechnol., 20, 1018, 2001
• Non-Patent Document 6: Genome Research, 12, 640, 2002
• Non-Patent Document 7: Genome Research, 13, 644, 2003

DISCLOSURE OF THE INVENTION

object of the Invention

It is an object of the present invention to provide an E. coli mutant strain that lacks a plurality of regions on chromosomal DNA and a process for producing a useful substance using such mutant.

Means for Attaining the Object

The present invention relates to the following (1) to (47). (1) An E. coli mutant strain comprising the following genes [1] and [2] or homologous genes thereof and comprising chromosomal DNA that is at least 470 kbp shorter than that of a wild-type E. coli strain, provided that when the following genes [1] and [2] or homologous genes thereof comprise genes that wild-type E. coli strains do not originally contain (hereafter referred to as “NC genes”), the aforementioned E. coli mutant strain derived from the wild-type E. coli strain does not necessarily have the NC gene. [1] thrA gene, thrB gene, thrC gene, talB gene, mog gene, htgA gene, dnaK gene, dnaJ gene, hokC gene, nhaA gene, nhaR gene, rpsT gene, ribF gene, ileS gene, lspA gene, fkpB gene, lytB gene, dapB gene, carA gene, carB gene, caiF gene, caiE gene, caiD gene, caiC gene, caiB gene, caiA gene, caiT gene, fixA gene, fixB gene, fixC gene, fixX gene, kefC gene, folA gene, apaH gene, apaG gene, ksgA gene, pdxA gene, surA gene, imp gene, djlA gene, rluA gene, hepA gene, polB gene, araD gene, araA gene, araB gene, araC gene, tbpA gene, leuD gene, leuC gene, leuB gene, leuA gene, leuL gene, leuO gene, ilvI gene, ilvH gene, fruR gene, ftsL gene, ftsI gene, murE gene, murF gene, mraY gene, murD gene, ftsW gene, murG gene, murC gene, ddlB gene, ftsQ gene, ftsA gene, ftsZ gene, lpxC gene, secA gene, mutT gene, yacE gene, guaC gene, hofC gene, hofB gene, ppdD gene, nadC gene, ampD gene, ampE gene, aroP gene, pdhR gene, aceE gene, aceF gene, lpdA gene, acnB gene, speD gene, speE gene, gcd gene, hpt gene, yadF gene, panD gene, panC gene, panB gene, htrE gene, ecpD gene, folK gene, pcnB gene, dksA gene, sfsA gene, ligT gene, hrpB gene, ponB gene, hemL gene, pfs gene, dgt gene, htrA gene, dapD gene, glnD gene, map gene, rpsB gene, tsf gene, smb gene, rrf gene, dxr gene, rth gene, cdsA gene, yaeL gene, hlpA gene, lpxD gene, fabZ gene, lpxA gene, lpxB gene, rnhB gene, dnaE gene, accA gene, ldcC gene, mesJ gene, rof gene, cutF gene, proS gene, rcsF gene, abc gene, dniR gene, gloB gene, rnhA gene, dnaQ gene, up18 gene, gmhA gene, pepD gene, gpt gene, crl gene, phoE gene, proB gene, proA gene, hemB gene, proC gene, aroL gene, aroM gene, araJ gene, sbcC gene, sbcD gene, phoB gene, phoR gene, bmQ gene, proY gene, malZ gene, queA gene, tgt gene, secD gene, secF gene, tsx gene, ribD gene, ribH gene, nusB gene, thiL gene, pgpA gene, dxs gene, ispA gene, xseB gene, thiI gene, thiJ gene, apbA gene, cyoE gene, cyoD gene, cyoC gene, cyoB gene, cyoA gene, ampG gene, bolA gene, tig gene, clpP gene, clpX gene, lon gene, hupB gene, cof gene, mdlA gene, mdlB gene, glnK gene, amtB gene, tesB gene, maa gene, hha gene, acrB gene, acrA gene, acrR gene, aefA gene, priC gene, apt gene, dnaX gene, recR gene, htpG gene, adk gene, hemH gene, aes gene, gsk gene, fsr gene, ushA gene, cueR gene, gcl gene, gip gene, purK gene, purE gene, ybbF gene, ppiB gene, cysS gene, folD gene, sfiA gene, sfmC gene, sfmD gene, sfmH gene, sfmF gene, fimZ gene, entD gene, fepA gene, fes gene, entF gene, fepE gene, fepC gene, fepG gene, fepD gene, fepB gene, entC gene, entE gene, entB gene, entA gene, cstA gene, dsbG gene, ahpC gene, ahpF gene, rnk gene, rna gene, citT gene, citG gene, citX gene, citF gene, citE gene, citD gene, citC gene, citA gene, citB gene, dcuC gene, dcuC gene, crcA gene, cspE gene, crcB gene, lipA gene, lipB gene, dacA gene, rlpA gene, mrdB gene, mrdA gene, phpB gene, nadD gene, holA gene, rlpB gene, leuS gene, lnt gene, phoL gene, ubiF gene, asnB gene, nagD gene, nagC gene, nagA gene, nagB gene, nagE gene, glnS gene, fur gene, fldA gene, seqA gene, pgm gene, potE gene, speF gene, kdpE gene, kdpD gene, kdpC gene, kdpB gene, kdpA gene, phrB gene, end8 gene, abrB gene, gltA gene, sdhC gene, sdhD gene, sdhA gene, sdhB gene, sucA gene, sucB gene, sucC gene, sucD gene, farR gene, hrsA gene, cydA gene, cydB gene, tolQ gene, tolR gene, tolA gene, tolB gene, pal gene, nadA gene, pnuC gene, czcD gene, aroG gene, gpmA gene, galM gene, galK gene, galT gene, galE gene, modF gene, modE gene, modA gene, modB gene, modC gene, bioA gene, bioB gene, bioF gene, bioC gene, bioD gene, uvrB gene, moaA gene, moaB gene, moaC gene, moaD gene, moaE gene, rhlE gene, dps gene, ompX gene, mipB gene, moeB gene, moeA gene, mdfA gene, grxA gene, poxB gene, aqpZ gene, cspD gene, cipA gene, infA gene, aat gene, cydC gene, cydD gene, trxB gene, lrp gene, ftsK gene, lolA gene, serS gene, dmsA gene, dmsB gene, dmsC gene, pflA gene, pflB gene, focA gene, serC gene, aroA gene, cmk gene, rpsA gene, himD gene, msbA gene, lpxK gene, kdsB gene, smtA gene, mukF gene, kicA gene, mukB gene, aspC gene, ompF gene, asnS gene, pncB gene, pepN gene, ssuB gene, ssuC gene, ssuD gene, ssuA gene, ssuE gene, pyrD gene, uup gene, pqiA gene, pqiB gene, rmf gene, fabA gene, ompA gene, sulA gene, helD gene, mgsA gene, acyP gene, hyaA gene, hyaB gene, hyaC gene, hyaD gene, hyaE gene, hyaF gene, appC gene, appB gene, appA gene, csgG gene, csgF gene, csgE gene, csgD gene, csgB gene, csgA gene, csgC gene, sfaA gene, mdoG gene, mdoH gene, msyB gene, htrB gene, solA gene, dinI gene, pyrC gene, grxB gene, rimJ gene, mviM gene, mviN gene, flgN gene, flgM gene, flgA gene, flgB gene, flgC gene, flgD gene, flgE gene, flgF gene, flgG gene, flgH gene, flgI gene, flgJ gene, flgK gene, flgL gene, rne gene, rluC gene, rpmF gene, plsX gene, fabH gene, fabD gene, fabG gene, acpP gene, fabF gene, pabC gene, tmk gene, holB gene, ptsG gene, fhuE gene, nagZ gene, ndh gene, mfd gene, ycfU gene, ycfV gene, ycfW gene, cobB gene, potD gene, potC gene, potB gene, potA gene, pepT gene, phoQ gene, phoP gene, purB gene, trmU gene, icdA gene, minE gene, minD gene, minC gene, clyA gene, umuD gene, umuC gene, dsbB gene, nhaB gene, fadR gene, dadA gene, dadX gene, ldcA gene, mltE gene, tag1 gene, treA gene, dhaR gene, pth gene, prsA gene, ychB gene, hemM gene, hemA gene, prfA gene, hemK gene, kdsA gene, chaA gene, chaB gene, chaC gene, narL gene, narX gene, narK gene, narG gene, narH gene, narJ gene, narI gene, tpr gene, purU gene, sprE gene, galU gene, hns gene, tdk gene, adhE gene, oppA gene, oppB gene, oppC gene, oppD gene, oppF gene, cls gene, kch gene, tonB gene, trpA gene, trpB gene, trpC gene, trpD gene, trpE gene, trpL gene, btuR gene, sohB gene, topA gene, cysB gene, acnA gene, ribA gene, pgpB gene, pyrF gene, osmB gene, rnb gene, envM gene, sapF gene, sapD gene, sapC gene, sapB gene, sapA gene, aldH gene, ordL gene, goaG gene, pspF gene, pspA gene, pspB gene, pspC gene, pspD gene, pspE gene, sucP gene, ompG gene, tyrR gene, tpx gene, mppA gene, fnr gene, ogt gene, dbpA gene, intR gene, lar gene, recT gene, recE gene, racC gene, kil gene, sieB gene, racR gene, dnaC gene, enpP gene, trkG gene, VMTH_LAMBD gene, VLOM_LAMBD gene, tnpO gene, upo3 gene, ompN gene, nifJ gene, hslJ gene, ldhA gene, feaR gene, feaB gene, tynA gene, paaZ gene, paaA gene, paaB gene, paaC gene, paaD gene, paaE gene, paaF gene, paaG gene, paaH gene, paaI gene, paaJ gene, paaK gene, paaX gene, paaY gene, acpD gene, hrpA gene, aldA gene, gapC gene, gapC gene, cybB gene, trg gene, rimL gene, tehA gene, tehB gene, prtC gene, potA gene, potC gene, ansP gene, nhoA gene, narV gene, narW gene, narY gene, narZ gene, narU gene, fdnG gene, fdnH gene, fdnI gene, adhP gene, sfcA gene, rpsV gene, osmC gene, dos gene, xasA gene, gadB gene, pqqL gene, chuR gene, hipA gene, hipB gene, tam gene, uxaB gene, marC gene, marR gene, marA gene, marB gene, dcp gene, lycV gene, dicA gene, dicB gene, rspB gene, rspA gene, speG gene, dmsB gene, dmsC gene, bioD gene, mlc gene, asr gene, ebr gene, pntB gene, pntA gene, arcD gene, rstA gene, rstB gene, tus gene, fumC gene, fumA gene, manA gene, uidC gene, uidB gene, uidA gene, uidR gene, hdhA gene, mall gene, malX gene, malY gene, add gene, blr gene, nth gene, gst gene, pdxY gene, tyrS gene, pdxH gene, slyB gene, slyA gene, sodC gene, nemA gene, gloA gene, rnt gene, lhr gene, sodB gene, araJ gene, purR gene, cfa gene, ribE gene, ag43 gene, phsC gene, nrfC gene, pykF gene, lpp gene, sufs gene, aroD gene, ppsA gene, aroH gene, nlpC gene, btuD gene, btuE gene, btuC gene, himA gene, pheT gene, pheS gene, pheM gene, rplT gene, rpml gene, infC gene, thrs gene, arp gene, pfkB gene, cedA gene, katE gene, celF gene, celD gene, ceiC gene, celB gene, celA gene, osmE gene, nadE gene, spy gene, astE gene, astB gene, astD gene, astA gene, cstC gene, xthA gene, gdhA gene, topB gene, selD gene, sppA gene, ansA gene, tdh gene, gapA gene, ttuC gene, caiT gene, rnd gene, fadD gene, slp gene, pabB gene, sdaA gene, manX gene, manY gene, manZ gene, rrmA gene, cspC gene, kdgR gene, tcmA gene, htpX gene, prc gene, pqiB gene, pphA gene, holE gene, exoX gene, ptrB gene, purT gene, eda gene, edd gene, zwf gene, pykA gene, mlt gene, znuA gene, znuC gene, ruvB gene, ruvA gene, ruvC gene, ntpA gene, aspS gene, bisZ gene, torY gene, cutC gene, argS gene, pgsA gene, a=m gene, cbl gene, nac gene, erfK gene, cobT gene, cobS gene, cobU gene, gyrI gene, phsE gene, sbcB gene, hisL gene, hisG gene, hisD gene, hisC gene, hisB gene, hisH gene, hisA gene, hisF gene, hisI gene, wzz gene, ugd gene, gnd gene, galF gene, wcaM gene, wcaL gene, wcaK gene, wzxC gene, wcaJ gene, cpsG gene, cpsB gene, wcaI gene, wcaH gene, wcaG gene, gmd gene, wcaF gene, wcaE gene, wcaD gene, wcaC gene, wcaB gene, wcaA gene, wzb gene, wza gene, asmA gene, dcd gene, udk gene, thiD gene, thiM gene, mrp gene, metG gene, cdd gene, sanA gene, mglC gene, mglA gene, mglB gene, galS gene, folE gene, cirA gene, lysP gene, nfo gene, fruA gene, fruK gene, fruB gene, rplY gene, narP gene, ccmH gene, dsbE gene, ccmF gene, ccmE gene, ccmD gene, ccmC gene, ccmB gene, ccmA gene, napC gene, napB gene, napH gene, napG gene, napA gene, napD gene, napF gene, eco gene, alkB gene, ada gene, apbE gene, rcsB gene, rcsC gene, atoS gene, atoC gene, atoD gene, atoA gene, atoE gene, atoB gene, gyrA gene, ubiG gene, nrdA gene, nrdB gene, inaA gene, glpQ gene, glpT gene, glpA gene, glpB gene, glpC gene, cinA gene, ais gene, pmrD gene, menE gene, menC gene, menB gene, menD gene, menF gene, nuoN gene, nuoM gene, nuoL gene, nuoK gene, nuoJ gene, nuoI gene, nuoH gene, nuoG gene, nuoF gene, nuoE gene, nuoD gene, nuoB gene, nuoA gene, lrhA gene, ackA gene, pta gene, ubiX gene, purF gene, dedE gene, dedD gene, foIC gene, accD gene, dedA gene, truA gene, usgI gene, pdxB gene, div gene, fabB gene, ddpX gene, mepA gene, aroC gene, sixA gene, fadL gene, vacJ gene, dsdC gene, dsdX gene, dsdA gene, emrY gene, emrK gene, evgA gene, evgS gene, bifF gene, ddg gene, glk gene, mntH gene, nupC gene, gltX gene, xapR gene, xapB gene, xapA gene, lig gene, zipA gene, cysZ gene, cysK gene, ptsH gene, ptsI gene, gsr gene, pdxK gene, cysM gene, cysA gene, cysW gene, cysU gene, cysP gene, ucpA gene, ptbA gene, amiA gene, hemF gene, cchA gene, eutC gene, eutB gene, eutH gene, eutG gene, eutJ gene, eutE gene, cchB gene, eutI gene, talA gene, tktB gene, aegA gene, narQ gene, acrD gene, dapE gene, purC gene, nlpB gene, dapA gene, gcvR gene, bcp gene, hyfA gene, hyfB gene, hyfC gene, hyfD gene, hyfE gene, hyfF gene, hyfG gene, hyfH gene, hyfI gene, hyfJ gene, hyfR gene, focB gene, perM gene, yfgE gene, uraA gene, upp gene, purM gene, purN gene, ppk gene, ppx gene, guaA gene, guaB gene, xseA gene, hisS gene, gcpE gene, ndk gene, pbpC gene, sseA gene, sseB gene, pepB gene, fdx gene, hscA gene, hscB gene, iscU gene, iscS gene, iscR gene, suhB gene, csiE gene, dgxA gene, dgxB gene, hcaC gene, hcaB gene, hcaD gene, glyA gene, hmpA gene, glnB gene, purL gene, acpS gene, pdxJ gene, recO gene, era gene, me gene, lepB gene, lepA gene, rseC gene, rseB gene, rseA gene, rpoE gene, nadB gene, srnB gene, ung gene, trx2 gene, pssA gene, kgtP gene, clpB gene, rluD gene, pheL gene, pheA gene, tyrA gene, aroF gene, rplS gene, trmD gene, rimM gene, rpsp gene, ffh gene, grpE gene, recN gene, smpA gene, smpB gene, gabD gene, gabT gene, gabP gene, nrdF gene, emrR gene, emrA gene, emrB gene, gshA gene, csrA gene, alaS gene, oraA gene, recA gene, mltB gene, hypF gene, hydN gene, ascG gene, ascF gene, ascB gene, hycI gene, hycH gene, hycG gene, hycF gene, hycE gene, hycD gene, hycC gene, hycB gene, hycA gene, hypA gene, hypB gene, hypC gene, hypD gene, hypE gene, fhlA gene, mutS gene, prpB gene, rpoS gene, pcm gene, surE gene, ygbB gene, ygbP gene, ygbQ gene, cysC gene, cysN gene, cysD gene, iap gene, cysH gene, cysI gene, cysJ gene, ptpS gene, eno gene, pyrG gene, mazG gene, chpR gene, relA gene, barA gene, gud2 gene, gudT gene, mioC gene, syd gene, sdaB gene, exo gene, fucO gene, fucA gene, fucP gene, fueI gene, fucK gene, fucU gene, fucR gene, gcvA gene, nifS gene, mitA gene, amiC gene, argA gene, thyA gene, lgt gene, ptsP gene, mutH gene, tas gene, aas gene, galR gene, lysA gene, lysR gene, araE gene, kduD gene, kduI gene, lysS gene, prfB gene, recJ gene, dsbC gene, xerD gene, fldB gene, upl4 gene, bglA gene, gcvP gene, gevH gene, gcvT gene, visC gene, ubiH gene, pepP gene, serA gene, rpiA gene, iciA gene, sbm gene, argK gene, fba gene, pgk gene, epd gene, tktA gene, speB gene, speA gene, metK gene, galP gene, sprT gene, endA gene, gshB gene, yqgF gene, ansB gene, mutY gene, mltC gene, nupG gene, speC gene, pitB gene, gsp gene, hybG gene, hybF gene, hybE gene, hybD gene, hybC gene, hybB gene, hybA gene, hoxK gene, exbD gene, exbB gene, metC gene, exsA gene, sufI gene, plsC gene, parC gene, parE gene, icc gene, tolC gene, ribB gene, glgS gene, rfaE gene, glnE gene, cca gene, bacA gene, folB gene, ttdA gene, ttdB gene, ygjD gene, rpsU gene, dnaG gene, rpoD gene, aer gene, ebgR gene, ebgA gene, ebgC gene, fadH gene, uxaA gene, uxaC gene, exuT gene, exuR gene, tdcE gene, tdcD gene, tdcC gene, tdcB gene, tdcA gene, tdcR gene, sohA gene, agaR gene, agaZ gene, agaV gene, agaW gene, agaA gene, agaS gene, agaY gene, agaB gene, agac gene, agaD gene, agaI gene, mtr gene, deaD gene, nlpI gene, pnp gene, rpsO gene, truB gene, rbfA gene, infB gene, nusA gene, argG gene, secG gene, mrsA gene, folP gene, ftsH gene, ftsJ gene, greA gene, dacB gene, yhbZ gene, rpmA gene, rplU gene, ispB gene, nlp gene, murA gene, yrbI gene, yrbK gene, rpoN gene, ptsN gene, ptsO gene, mtgA gene, arcB gene, gltB gene, nanT gene, npl gene, nanR gene, dcuD gene, sspB gene, sspA gene, rpsI gene, rplM gene, hhoA gene, hhoB gene, md& gene, argR gene, tldD gene, rmg gene, mreD gene, mreC gene, mreB gene, accB gene, accC gene, panF gene, prmA gene, fis gene, aroE gene, smg gene, smf gene, def gene, fmt gene, sun gene, trkA gene, mscL gene, zntR gene, rplQ gene, rpoA gene, rpsD gene, rpsK gene, rpsM gene, rpmJ gene, secY gene, rplO gene, rpmD gene, rpsE gene, rpIR gene, rplF gene, rpsH gene, rpsN gene, rplE gene, rplX gene, rplN gene, rpsQ gene, rpmC gene, rplP gene, rpsC gene, rpIV gene, rpsS gene, rplB gene, rplW gene, rplD gene, rplC gene, rpsJ gene, tufA gene, fusA gene, rpsG gene, rpsL gene, fkpA gene, slyX gene, slyD gene, kefB gene, prkB gene, crp gene, argD gene, pabA gene, fic gene, ppiA gene, nirB gene, nirD gene, nirC gene, cysG gene, php gene, trpS gene, gph gene, rpe gene, dam gene, damX gene, aroB gene, aroK gene, hofQ gene, mrcA gene, pckA gene, envZ gene, ompR gene, greB gene, feoA gene, feoB gene, bioH gene, gntT gene, malQ gene, malP gene, malT gene, rtcA gene, rtcB gene, rtcR gene, glpR gene, gipG gene, gIpE gene, glpD gene, glgP gene, glgA gene, glgC gene, glgX gene, glgB gene, asd gene, gntU gene, gntU gene, gntK gene, gntR gene, ggt gene, ugpQ gene, ugpC gene, ugpE gene, ugpA gene, ugpB gene, livF gene, livG gene, livM gene, livH gene, livK gene, livJ gene, rpoH gene, ftsX gene, ftsE gene, ftsY gene, atzN gene, nikA gene, nikB gene, nikC gene, nikD gene, nikE gene, pitA gene, uspB gene, uspA gene, prlC gene, gor gene, arsR gene, arsB gene, arsC gene, slp gene, hdeB gene, hdeA gene, hdeD gene, gadA gene, treF gene, kdgK gene, dctA gene, dppF gene, dppD gene, dppC gene, dppB gene, dppA gene, tag gene, bisC gene, cspA gene, glyS gene, glyQ gene, xylB gene, xylA gene, xylF gene, xylG gene, xylH gene, xylR gene, bax gene, malS gene, avtA gene, lyx gene, sgbH gene, sgbU gene, sgbE gene, aldB gene, selB gene, selA gene, mtlA gene, mtlD gene, mtlR gene, lldP gene, lldR gene, lldD gene, cysE gene, gpsA gene, secB gene, grxC gene, tdh gene, kbl gene, htrL gene, rfaD gene, rfaF gene, rfaC gene, rfaL gene, rfaK gene, rfaZ gene, rfaY gene, rfaJ gene, rfaI gene, rfaB gene, rfaS gene, rfaP gene, rfaG gene, rfaQ gene, kdtA gene, kdtB gene, mutM gene, rpmG gene, rpmB gene, radC gene, dfp gene, dut gene, ttk gene, pyrE gene, rph gene, dinD gene, gmk gene, rpoZ gene, spoT gene, spoU gene, recG gene, gltS gene, nlpA gene, uhpT gene, uhpC gene, uhpB gene, uhpA gene, ilvN gene, ilvB gene, ivbL gene, emrD gene, ibpB gene, ibpA gene, gyrB gene, recF gene, dnaN gene, dnaA gene, rpmH gene, rnpA gene, yidC gene, thdF gene, tnaL gene, tnaA gene, tnaB gene, bglB gene, bglF gene, bglG gene, phoU gene, pstB gene, pstA gene, pstC gene, pstS gene, glmS gene, glmU gene, atpC gene, uncD gene, atpG gene, atpA gene, atpH gene, atpF gene, atpE gene, atpB gene, atpI gene, gidB gene, gidA gene, mioC gene, asnC gene, pssR gene, ilvL gene, ilvG gene, ilvM gene, ilvE gene, ilvD gene, ilvA gene, ilvY gene, ilvC gene, ppiC gene, rep gene, gppA gene, rhIB gene, trxA gene, rhoL gene, rho gene, rfe gene, wzzE gene, nfrC gene, rffD gene, rffG gene, rffH gene, rffA gene, wzxE gene, rffT gene, wecG gene, aslB gene, aslA gene, hemY gene, hemX gene, hemD gene, hemC gene, cyaA gene, cyaY gene, dapF gene, xerC gene, uvrD gene, corA gene, radD gene, pldA gene, recQ gene, pldB gene, metR gene, metE gene, udp gene, ubiE gene, aarF gene, tatC gene, tatD gene, rfaH gene, ubiD gene, ubiB gene, fadA gene, fadB gene, pepQ gene, trkH gene, trkH gene, hemG gene, mobB gene, mobA gene, dsbA gene, polA gene, yihA gene, hemN gene, ntrC gene, ntrB gene, glnA gene, fdhE gene, fdoI gene, fdoH gene, fdoG gene, fdhD gene, sodA gene, kdgT gene, cpxA gene, cpxR gene, cpxP gene, pfkA gene, sbp gene, cdh gene, tpiA gene, fpr gene, glpX gene, glpK gene, glpF gene, menG gene, menA gene, hslU gene, hsiV gene, ftsN gene, cytR gene, priA gene, rpmE gene, metJ gene, metB gene, metL gene, metF gene, katG gene, gldA gene, talC gene, ppc gene, argE gene, argC gene, argB gene, argH gene, oxyR gene, udhA gene, trnA gene, btuB gene, murI gene, murB gene, birA gene, coaA gene, tufB gene, secE gene, nusG gene, rplK gene, rplA gene, rplJ gene, rplL gene, rpoB gene, rpoC gene, htrC gene, thiH gene, thiG gene, thiS gene, thiF gene, thiE gene, thiC gene, rsd gene, hemE gene, nfi gene, hupA gene, hydH gene, hydG gene, purD gene, purH gene, metA gene, aceB gene, aceA gene, aceK gene, arp gene, iclR gene, metH gene, pepE gene, lysC gene, pgi gene, ubiC gene, ubiA gene, plsB gene, dgkA gene, lexA gene, dinF gene, qor gene, dnaB gene, alr gene, tyrB gene, napA gene, hobH gene, uvrA gene, ssb gene, soxS gene, soxR gene, acs gene, nrfA gene, nrfB gene, nrfC gene, nrfD gene, nrfe gene, nrfF gene, nrfG gene, gltP gene, fdhF gene, alsR gene, rpiB gene, proP gene, basS gene, basR gene, adiY gene, adi gene, meiR gene, melA gene, melB gene, fumB gene, dcuB gene, dcuR gene, dcuS gene, lysU gene, cadA gene, cadB gene, cadC gene, cutA3 gene, cutA2 gene, cutA gene, dcuA gene, aspA gene, fxsA gene, groES gene, groEL gene, efp gene, ecnB gene, sugE gene, blc gene, ampC gene, frdD gene, frdC gene, frdB gene, frdA gene, genX gene, psd gene, yjeQ gene, yjeE gene, amiB gene, mutL gene, miaA gene, hfq gene, hflX gene, hflK gene, hflC gene, purA gene, vacB gene, sgaE gene, rpsF gene, priB gene, rpsR gene, rplI gene, fklB gene, cycA gene, cpdB gene, cysQ gene, msrA gene, chpS gene, chpB gene, ppa gene, fbp gene, mpl gene, pmbA gene, cybC gene, nrdG gene, nrdD gene, treC gene, treB gene, treR gene, mgtA gene, pyrI gene, pyrB gene, pyrBI gene, argI gene, valS gene, holC gene, xerB gene, idnR gene, idnT gene, idnO gene, idnD gene, idnK gene, fecE gene, fecD gene, fecC gene, fecB gene, fecA gene, fecR gene, feel gene, dnaC gene, dnaT gene, bglJ gene, fhuF gene, rsmC gene, holD gene, rimI gene, prfC gene, osmY gene, deoC gene, deoA gene, deoB gene, deoD gene, IplA gene, smp gene, serB gene, radA gene, nadR gene, slt gene, trpR gene, gpmG gene, rob gene, creA gene, creB gene, creC gene, creD gene, arcA gene, lasT gene, thrL gene;

[2] acrE gene, argT gene, artI gene, artJ gene, artM gene, artP gene, artQ gene, bfr gene, chiA gene, chpA gene, dacC gene, deoR gene, dinG gene, elaA gene, elaB gene, elaC gene, elaD gene, envD gene, envR gene, fhuA gene, fhuB gene, fhuC gene, fhuD gene, foIX gene, glnH gene, glnP gene, glnQ gene, gltJ gene, gltK gene, gltL gene, gspA gene, gspc gene, gspD gene, gspE gene, gspI gene, gspJ gene, gspK gene, gspL gene, hisJ gene, hisM gene, hisP gene, hisQ gene, hofD gene, hofF gene, hofG gene, hofH gene, ltaA gene, mdaA gene, mdaB gene, pinO gene, potF gene, potG gene, potH gene, potI gene, pshM gene, ptwB gene, ptwC gene, rhsB gene, rimK gene, xylU gene.

(2) The E. coli mutant strain according to above (1) comprising chromosomal DNA that comprises DNA having the nucleotide sequences as shown in [1] to [69] below of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.

• [1] a nucleotide sequence of nucleotides 1 to 167064
• [2] a nucleotide sequence of nucleotides 172980 to 243964
• [3] a nucleotide sequence of nucleotides 253411 to 261963
• [4] a nucleotide sequence of nucleotides 387532 to 389138
• [5] a nucleotide sequence of nucleotides 403704 to 518029
• [6] a nucleotide sequence of nucleotides 532712 to 535504
• [7] a nucleotide sequence of nucleotides 550216 to 563940
• [8] a nucleotide sequence of nucleotides 608119 to 675103
• [9] a nucleotide sequence of nucleotides 689375 to 728820
• [10] a nucleotide sequence of nucleotides 739594 to 832551
• [11] a nucleotide sequence of nucleotides 848090 to 867637
• [12] a nucleotide sequence of nucleotides 883474 to 890997
• [13] a nucleotide sequence of nucleotides 909380 to 1048887
• [14] a nucleotide sequence of nucleotides 1097020 to 1198105
• [15] a nucleotide sequence of nucleotides 1225147 to 1630911
• [16] a nucleotide sequence of nucleotides 1641039 to 1642631
• [17] a nucleotide sequence of nucleotides 1650223 to 1964523
• [18] a nucleotide sequence of nucleotides 1993076 to 1996661
• [19] a nucleotide sequence of nucleotides 2028210 to 2033448
• [20] a nucleotide sequence of nucleotides 2044377 to 2069148
• [21] a nucleotide sequence of nucleotides 2081927 to 2104392
• [22] a nucleotide sequence of nucleotides 2116065 to 2146263
• [23] a nucleotide sequence of nucleotides 2187856 to 2200668
• [24] a nucleotide sequence of nucleotides 2229851 to 2255894
• [25] a nucleotide sequence of nucleotides 2263492 to 2270439
• [26] a nucleotide sequence of nucleotides 2286589 to 2386261
• [27] a nucleotide sequence of nucleotides 2395472 to 2423364
• [28] a nucleotide sequence of nucleotides 2434099 to 2472851
• [29] a nucleotide sequence of nucleotides 2482483 to 2505074
• [30] a nucleotide sequence of nucleotides 2514558 to 2755676
• [31] a nucleotide sequence of nucleotides 2789481 to 2795173
• [32] a nucleotide sequence of nucleotides 2800891 to 2804307
• [33] a nucleotide sequence of nucleotides 2810148 to 2825313
• [34] a nucleotide sequence of nucleotides 2830263 to 2910273
• [35] a nucleotide sequence of nucleotides 2910574 to 2985361
• [36] a nucleotide sequence of nucleotides 3033129 to 3077084
• [37] a nucleotide sequence of nucleotides 3078917 to 3110109
• [38] a nucleotide sequence of nucleotides 3134342 to 3165627
• [39] a nucleotide sequence of nucleotides 3172941 to 3360736
• [40] a nucleotide sequence of nucleotides 3370548 to 3411159
• [41] a nucleotide sequence of nucleotides 3420879 to 3453020
• [42] a nucleotide sequence of nucleotides 3469367 to 3618575
• [43] a nucleotide sequence of nucleotides 3636917 to 3854661
• [44] a nucleotide sequence of nucleotides 3865832 to 3867101
• [45] a nucleotide sequence of nucleotides 3877011 to 3926658
• [46] a nucleotide sequence of nucleotides 3938879 to 4059897
• [47] a nucleotide sequence of nucleotides 4079660 to 4086450
• [48] a nucleotide sequence of nucleotides 4099983 to 4139231
• [49] a nucleotide sequence of nucleotides 4149710 to 4235358
• [50] a nucleotide sequence of nucleotides 4251736 to 4298976
• [51] a nucleotide sequence of nucleotides 4311512 to 4313317
• [52] a nucleotide sequence of nucleotides 4329722 to 4409532
• [53] a nucleotide sequence of nucleotides 4423152 to 4496201
• [54] a nucleotide sequence of nucleotides 4509535 to 4517875
• [55] a nucleotide sequence of nucleotides 4599638 to 4641433
• [56] a nucleotide sequence of nucleotides 167065 to 172979
• [57] a nucleotide sequence of nucleotides 675104 to 689374
• [58] a nucleotide sequence of nucleotides 832552 to 848089
• [59] a nucleotide sequence of nucleotides 867638 to 883473
• [60] a nucleotide sequence of nucleotides 890998 to 909379
• [61] a nucleotide sequence of nucleotides 2386262 to 2395471
• [62] a nucleotide sequence of nucleotides 2423365 to 2434098
• [63] a nucleotide sequence of nucleotides 2472852 to 2482482
• [64] a nucleotide sequence of nucleotides 2505075 to 2514557
• [65] a nucleotide sequence of nucleotides 2910274 to 2910573
• [66] a nucleotide sequence of nucleotides 3165628 to 3172940
• [67] a nucleotide sequence of nucleotides 3411160 to 3420878
• [68] a nucleotide sequence of nucleotides 3453021 to 3469366
• [69] a nucleotide sequence of nucleotides 3618576 to 3636916

(3) The E. coli mutant strain according to (1) or (2) comprising chromosomal DNA that does not comprise yafJ gene, yafK gene, yafQ gene, dinJ gene, yafL gene, yafM gene, fhiA gene, lafU gene, dinP gene, yafN gene, yafO gene, yafP gene, JW0225 gene, prfH gene, ykfI gene, yafW gene, yeeT gene, ykfG gene, yafX gene, ykfF gene, ykfB gene, yafY gene, ypjK gene, yafZ gene, ykfA gene, perR gene, JW0245 gene, JW0246 gene, JW0247 gene, ykfC gene, JW0249 gene, is5B gene, yi51 gene, mmuP gene, mmuM gene, afuC gene, JW0255 gene, insB gene, insA gene, JW0258 gene, yagB gene, yagA gene, yagE gene, yagF gene, yagG gene, yagH gene, yagI gene, argF gene, yfjI gene, yagJ gene, yagK gene, yagL gene, yagM gene, yagN gene, intF gene, yagP gene, yagQ gene, yagR gene, yagS gene, yagT gene, yagU gene, ykgJ gene, yagV gene, yagW gene, yagX gene, yagY gene, yagZ gene, ykgK gene, ykgL gene, ykgM gene, eaeH gene, JW0292 gene, JW0293 gene, ykgA gene, ykgB gene, ykgI gene, merA gene, ykgD gene, ykgE gene, ykgF gene, ykgG gene, ykgH gene, betA gene, betB gene, betI gene, betT gene, yahA gene, yahB gene, yahC gene, yahD gene, yahE gene, yahF gene, yahG gene, yahH gene, arcM gene, yahJ gene, yahK gene, yahL gene, yahM gene, yahN gene, yahO gene, prpR gene, bcpA gene, prpC gene, prpD gene, prpE gene, codB gene, codA gene, cynR gene, cynT gene, cynS gene, cynX gene, lacA gene, lacY gene, lacZ gene, lacI gene, mhpR gene, mhpA gene, mhpB gene, mhpC gene, mhpD gene, mhpF gene, mhpE gene, mhpT gene, yaiL gene, yaiM gene, adhC gene, yaiN gene, yaiO gene, JW0350 gene, yi2A gene, yi22 gene, yi24 gene, yi23 gene, yaiP gene, yaiS gene, tauA gene, tauB gene, tauC gene, ssiD gene, yaiT gene, JW0363 gene, JW0364 gene, yaiU gene, yaiV gene, ampH gene, sbmA gene, yaiW gene, yaiY gene, yaiZ gene, ddlA gene, yaiB gene, phoA gene, psiF gene, yaiC gene, ybbO gene, tesA gene, ybbA gene, ybbP gene, rhsD gene, ybbC gene, rhsE gene, ybbD gene, JW0490 gene, ybbB gene, ybbS gene, ybbT gene, ybbU gene, ybbQ gene, ybbV gene, allP gene, allA gene, ybbY gene, ybbZ gene, ylbA gene, allC gene, ylbC gene, fdrA gene, ylbE gene, ylbF gene, arcC gene, intD gene, EXO_LAMBD gene, VRPP_LAMBD gene, JW0528 gene, JW0529 gene, VREN_LAMBD gene, emrE gene, ybcK gene, ybcL gene, ybcM gene, ybcN gene, ninE gene, ybcO gene, rus gene, ybcQ gene, JW0539.5 gene, nmpC gene, vlyS gene, ybcS gene, ybcT gene, vboR gene, ybcV gene, ybcW gene, nohB gene, ybcX gene, ybcY gene, JW0552 gene, appY gene, ompT gene, envY gene, ybcH gene, nfrA gene, nfrB gene, cusS gene, cusR gene, cusC gene, ylcC gene, cusB gene, cusA gene, pheP gene, ybdG gene, nfnB gene, ybdF gene, ybdJ gene, ybdK gene, hokE gene, yi81 gene, yi82 gene, ymcC gene, ymcD gene, cspH gene, cspG gene, sfa gene, yccL gene, yccM gene, torS gene, torT gene, torR gene, torC gene, torA gene, torD gene, yccD gene, cbpA gene, yccE gene, agp gene, yccJ gene, wrbA gene, yciG gene, ycdG gene, JW0992 gene, JW0993 gene, JW0994 gene, ycdK gene, JW0996 gene, JW0997 gene, ycdC gene, putA gene, yzpU gene, putP gene, JW1002 gene, ycdO gene, ycdB gene, phoH gene, ycdP gene, ycdQ gene, ycdR gene, ycdS gene, ycdT gene, JW1012 gene, JW1013 gene, JW1014 gene, ycdU gene, ymfD gene, ymfE gene, lit gene, intE gene, VXIS_BPP21 gene, ymfH gene, ymfI gene, ymfJ gene, RPC2_BPP22 gene, JW1132 gene, ymfL gene, ymfM gene, ymfN gene, ymfR gene, ymfO gene, JW1138 gene, JW1139 gene, ycfK gene, ymfS gene, ycfA gene, ycfE gene, pin gene, mcrA gene, JW1146 gene, ycgW gene, ycgX gene, ycgE gene, JW1150 gene, ycgZ gene, ymgA gene, JW1153 gene, ymgC gene, yahA gene, JW1156 gene, JW1157 gene, aidA gene, ymgD gene, JW1160 gene, JW1161 gene, JW1162 gene, ydfH gene, ydfZ gene, ydfI gene, shiA gene, JW1537 gene, tnpR gene, ydfM gene, Y314_LAMBD gene, nohA gene, ydfO gene, yccL gene, cspI gene, ydfR gene, vlyS gene, cspB gene, cspF gene, regQ_LAMBD gene, JW1552 gene, rem gene, hokD gene, relE gene, relB gene, ydfV gene, flxA gene, ydfW gene, ydfX gene, dicC gene, sdaC gene, yqeH gene, yqeI gene, yqeJ gene, yqeK gene, ygeF gene, ygeG gene, ygeH gene, ygeI gene, JW2822 gene, ygeK gene, JW2824 gene, JW2825 gene, JW2830 gene, ygeP gene, JW2832 gene, nlpD gene, yagR gene, ygeT gene, JW2836 gene, ygeV gene, ygeW gene, dpaL gene, ygeY gene, ygeZ gene, arcL gene, yqeB gene, JW2844 gene, ygfJ gene, gltD gene, JW2847 gene, ygfM gene, yagR gene, ygfO gene, ygfP gene, ygfQ gene, ygfR gene, ygfS gene, ygfT gene, pbuX gene, idi gene, epsM gene, exeL gene, exeC gene, JW2938 gene, JW2939 gene, JW2940 gene, JW2941 gene, yghK gene, glcB gene, glcG gene, glcF gene, glcD gene, glcC gene, JW2948 gene, yghQ gene, yghR gene, yghS gene, yghT gene, yihL gene, yihM gene, yihN gene, yshA gene, yihO gene, yihP gene, yihQ gene, yihR gene, yihS gene, yihT gene, yihU gene, yihV gene, yihW gene, yihX gene, rbn gene, yihZ gene, yiiD gene, yiiE gene, yiiF gene, yiiG gene, frvR gene, frvX gene, frvB gene, frvA gene, yiiL gene, rhaD gene, rhaA gene, rhaB gene, rhaS gene, rhaR gene, rhaT gene, ptsA gene, JW3920 gene, frwC gene, frwB gene, pflD gene, pflC gene, frwD gene, yijO gene, yijP gene, JW3986 gene, yjbF gene, yjbG gene, yjbH gene, JW3989.5 gene, yjbA gene, xylE gene, malG gene, malF gene, malE gene, malK gene, lamB gene, malM gene, yjbI gene, yjcP gene, yjcQ gene, yjcR gene, yjcS gene, alsK gene, alsE gene, alsC gene, alsA gene, alsB gene, phnQ gene, phnP gene, phnO gene, phnN gene, phnM gene, phnL gene, phnK gene, phnJ gene, phnI gene, phnH gene, phnG gene, phnF gene, phnE gene, phnD gene, phnC gene, phnB gene, phnA gene, yjdA gene and yjcZ gene, or homologous gene thereof.

(4) The E. coli mutant strain according to any one of (1) to (3) comprising chromosomal DNA that does not comprise DNA having the nucleotide sequences as shown in [1] to [18] below of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.

• [1] a nucleotide sequence of nucleotides 243965 to 253410
• [2] a nucleotide sequence of nucleotides 261964 to 387531
• [3] a nucleotide sequence of nucleotides 389139 to 403703
• [4] a nucleotide sequence of nucleotides 518030 to 532711
• [5] a nucleotide sequence of nucleotides 535505 to 550215
• [6] a nucleotide sequence of nucleotides 563941 to 608118
• [7] a nucleotide sequence of nucleotides 1048888 to 1097019
• [8] a nucleotide sequence of nucleotides 1198106 to 1225146
• [9] a nucleotide sequence of nucleotides 1630912 to 1641038
• [20] a nucleotide sequence of nucleotides 1642632 to 1650222
• [11] a nucleotide sequence of nucleotides 2985362 to 3033128
• [12] a nucleotide sequence of nucleotides 3110110 to 3134341
• [13] a nucleotide sequence of nucleotides 4059898 to 4079659
• [14] a nucleotide sequence of nucleotides 4086451 to 4099982
• [15] a nucleotide sequence of nucleotides 4139232 to 4149709
• [16] a nucleotide sequence of nucleotides 4235359 to 4251735
• [17] a nucleotide sequence of nucleotides 4298977 to 4311511
• [18] a nucleotide sequence of nucleotides 4313318 to 4329721

(5) The E. coli mutant strain according to any one of (1) to (4) comprising chromosomal DNA that does not comprise yidF gene, yidG gene, yidH gene, yidI gene, yidJ gene, yidK gene, yidL gene, JW3657 gene, glvG gene, glvB gene, glvC gene, yidP gene, yidE gene, yidQ gene, yidR gene, yidS gene, dgoT gene, dgoA gene, dgoK gene, dgoR gene, yidW gene, yidX gene, yidA gene, yidB gene, asnA gene, yieM gene, JW3724 gene, yieN gene, kup gene, rbsD gene, rbsA gene, rbsC gene, rbsB gene, rbsK gene, rbsR gene and yieO gene, or homologous gene thereof.

(6) The E. coli mutant strain according to any one of (1) to (5) comprising chromosomal DNA that does not comprise DNA having the nucleotide sequences as shown in [1] to [3] below of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.

• [1] a nucleotide sequence of nucleotides 3854662 to 3865831
• [2] a nucleotide sequence of nucleotides 3 867102 to 3877010
• [3] a nucleotide sequence of nucleotides 3926659 to 3938878

(7) The E. coli mutant strain according to any one of (1) to (6) comprising chromosomal DNA that does not comprise yecT gene, flhE gene, flhA gene, flhB gene, cheZ gene, cheY gene, cheB gene, cheR gene, tap gene, tar gene, cheW gene, cheA gene, motB gene, motA gene, flhC gene, flhD gene, JW1882 gene, JW1883 gene, yecG gene, otsA gene, otsB gene, araH gene, araG gene, araF gene, yecI gene, yecJ gene, JW1892 gene, ftn gene, yecH gene, tyrP gene, uvrC gene, uvrY gene, yecF gene, sdiA gene, yecC gene, yecS gene, yedO gene, fliY gene, fliZ gene, fliA gene, fliC gene, fliD gene, fliS gene, fliT gene, amyA gene, yedD gene, yedE gene, yedF gene, yedK gene, yedL gene, yedN gene, yedM gene, fliE gene, fliF gene, fliG gene, fliH gene, fliI gene, fliJ gene, fliK gene, fliL gene, fliM gene, fliN gene, fliO gene, fliP gene, fliQ gene, fliR gene, rcsA gene, dsrB gene, JW1937 gene, yedA gene, vsr gene, dcm gene, yedJ gene, JW1946 gene, ompC gene, JW1948 gene, yedS gene, yedU gene, yedV gene, copR gene, yedX gene, JW1954 gene, JW1955 gene, JW1956 gene, JW1974 gene, JW1979 gene, JW1980 gene, yeeP gene, flu gene, JW1983 gene, yeeS gene, yeeU gene, yeeV gene, yeeW gene, yefJ gene, JW2013 gene, yefJ gene, yefI gene, wbbJ gene, yefG gene, rfc gene, glf gene, rfbX gene, rfbC gene, rfbA gene, rfbD gene and rfbB gene, or homologous gene thereof.

(8) The E. coli mutant strain according to any one of (1) to (7) comprising chromosomal DNA that does not comprise DNA having any of the nucleotide sequences as shown in [1] to [5] below of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.

• [1] a nucleotide sequence of nucleotides 1964524 to 1993075
• [2] a nucleotide sequence of nucleotides 1996662 to 2028209
• [3] a nucleotide sequence of nucleotides 2033449 to 2044376
• [4] a nucleotide sequence of nucleotides 2069149t to 2081926
• [5] a nucleotide sequence of nucleotides 2104393 to 2116064

(9) The E. coli mutant strain according to any one of (1) to (8) comprising chromosomal DNA that does not comprise intA gene, yfjH gene, alpA gene, yfjI gene, JW2606 gene, yfjJ gene, yfjK gene, yfjL gene, yfjM gene, yfjN gene, yfjO gene, yfjP gene, yfjQ gene, yfjR gene, ypjK gene, yfjS gene, yfjT gene, yfjU gene, yfjV gene, JW2622 gene, yfjW gene, JW2623.5 gene, yfjX gene, yfjY gene, yfjZ gene, ypjF gene, ypjA gene, JW2629 gene, JW2630 gene, JW2631 gene, JW2632 gene, JW2633 gene, JW2634 gene, ygaE gene, yzzM gene, yqaE gene, JW2642 gene, ygaP gene, stpA gene, JW2645 gene, ygaC gene, ygaM gene, nrdH gene, nrdI gene, nrdE gene, proV gene, proW gene, proX gene, JW2655 gene, JW2656 gene, ygaZ gene, ygaH gene, srlA gene, srlB gene, srlD gene, gutM gene, srlR gene and gutQ gene, or homologous gene thereof.

(10) The E. coli mutant strain according to any one of (1) to (9) comprising chromosomal DNA that does not copmrise DNA having the nucleotide sequences as shown in [1] to [4] below of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.

• [1] a nucleotide sequence of nucleotides 2755677 to 2789480
• [2] a nucleotide sequence of nucleotides 2795174 to 2800890
• [3] a nucleotide sequence of nucleotides 2804308 to 2810147
• [4] a nucleotide sequence of nucleotides 2825314 to 2830262

(11) The E. coli mutant strain according to any one of (1) to (10) comprising chromosomal DNA that does not comprise gltF gene, yhcA gene, yhcD gene, yhcE gene, yhcF gene, yhcG gene, yhcH gene, nanK gene and nanE gene, or homologous gene thereof.

(12) The E. coli mutant strain according to any one of (1) to (11) comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 3360737 to 3370547 of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.

(13) The E. coli mutant strain according to any one of (1) to (12) comprising chromosomal DNA that does not comprise yegE gene, alkA gene, yegD gene, JW2055 gene, JW2056 gene, JW2057 gene, JW2058 gene, yegM gene, yegN gene, yegO gene, yegB gene, baeS gene, baeR gene, JW2065 gene, yegQ gene, ogrK gene, JW2068 gene, JW2069 gene, yegS gene, gatR gene, JW2072 gene, JW2073 gene, gatR gene, gatD gene, gatC gene, gatB gene, gatA gene, gatZ gene, gatY gene, fbaB gene, xegT gene, yegU gene, yegV gene, yegW gene, yegX gene, molR gene, yehI gene, yehK gene, yehL gene, yehO gene, yehP gene, yehQ gene, yehR gene, yehS gene, yehT gene, yehU gene, yehV gene, yehW gene, yehX gene, yehY gene, yehZ gene, bglX gene, dld gene, pbpG gene, yohC gene, yeiQ gene, yeiR gene, yeiU gene, spr gene, rtn gene, yejA gene, yejB gene, yejE gene, yejF gene, yejG gene, bcr gene, rsuA gene, yejH gene, yeiI gene, yeiJ gene, yeiK gene, yeiL gene, yeiM gene, yeiN gene and yeiC gene, or homologous gene thereof.

(14) The E. coli mutant strain according to any one of (1) to (13) comprising chromosomal DNA that does not comprise DNA having the nucleotide sequences as shown in [1] to [4] below of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.

• [1] a nucleotide sequence of nucleotides 2146264 to 2187855
• [2] a nucleotide sequence of nucleotides 2200669 to 2229850
• [3] a nucleotide sequence of nucleotides 2270440 to 2286588
• [4] a nucleotide sequence of nucleotides 2255895 to 2263491

(15) The E. coli mutant strain according to any one of (1) to (14) comprising chromosomal DNA that does not comprise cmtA gene, cmtB gene, or homologous gene thereof.

(16) The E. coli mutant strain according to any one of (1) to (15) comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 3077085 to 3078916 of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.

(17) The E. coli mutant strain according to any one of (1) to (16) comprising chromosomal DNA that does not comprise intB gene, JW4228 gene, yjgW gene, yjgX gene, yjgY gene, yjgZ gene, yi41 gene, yi42 gene, yjhB gene, yjhC gene, yjhD gene, yjhE gene, yi91 gene, JW4244 gene, JW4245 gene, JW4246 gene, yjhU gene, yjhF gene, yjhG gene, yjhH gene, yjhI gene, sgcR gene, sgcE gene, sgcA gene, sgcQ gene, sgcC gene, JW4266.5 gene, sgcX gene, yjhP gene, yjhQ gene, JW4269.5 gene, JW4270 gene, JW4270.5 gene, yjhR gene, yjhS gene, yjhT gene, yjhA gene, fimB gene, fimE gene, fimA gene, fimI gene, fimC gene, fimD gene, fimF gene, fimG gene, fimH gene, gntP gene, uxuA gene, uxuB gene, uxuR gene, yjiC gene, yjiD gene, yjiE gene, iadA gene, yjiG gene, yjiH gene, yjiI gene, yjiJ gene, yjiK gene, JW4296.5 gene, yjiL gene, yjiM gene, yjiN gene, yjiO gene, yjiP gene, yjiQ gene, yjiR gene, yjiS gene, yjiT gene, yjiV gene, mcrD gene, mcrC gene, mcrB gene, yjiW gene, hsdS gene, hsdM gene, hsdR gene, mrr gene, yjiA gene, yjiX gene, yjiY gene, tsr gene, yjjL gene, JW4320 gene, yjjM gene, yjjN gene, mdoB gene and yjjA gene, or homologous gene thereof.

(18) The E. coli mutant strain according to any one of (1) to (17) comprising chromosomal DNA that does not comprise DNA having the nucleotide sequences as shown in [1] or [2] below of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.

• [1] a nucleotide sequence of nucleotides 4496202 to 4509534
• [2] a nucleotide sequence of nucleotides 4517876 to 4599637

(19) The E. coli mutant strain according to any one of (1) to (18) comprising chromosomal DNA that does not comprise kdpF gene, ybfA gene, rhsC gene, JW0690 gene, ybfB gene, rhsA gene, ybfC gene, ybfD gene, ybfL gene, ybfD gene and ybgA gene, or homologous gene thereof.

(20) The E. coli mutant strain according to any one of (1) to (19) comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 728821 to 739593 of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.

(21) The E. coli mutant strain according to any one of (1) to (20) comprising chromosomal DNA that does not comprise yjfI gene, yjfJ gene, yjfK gene, yjfL gene, yjfM gene, yjfC gene, aidB gene, yjfN gene, yjfO gene, yjfP gene, yjfQ gene, yjfR gene, sgaT gene, sgaB gene, sgaA gene, sgaH gene and sgaU gene, or homologous gene thereof.

(22) The E. coli mutant strain according to any one of (1) to (21) comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 4409533 to 4423151 of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.

(23) An E. coli mutant strain comprising the gene (1) [1] above, spr gene, bcr gene, rsuA gene, or homologous gene thereof and comprising chromosomal DNA that is at least 933 kbp shorter than that of a wild-type E. coli strain.

(24) The E. coli mutant strain according to (23) comprising chromosomal DNA that comprise DNA having the nucleotide sequences as shown in [1] to [55] of (2) above and DNA having a nucleotide sequence comprising nucleotides 2270440 to 2286588 of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.

(25) The E. coli mutant strain according to (23) or (24) comprising chromosomal DNA that does not comprise yafJ gene, yafK gene, yafQ gene, dinJ gene, yafL gene, yafM gene, fhiA gene, lafU gene, dinP gene, yafN gene, yafO gene, yafP gene, JW0225 gene, prffH gene, ykfI gene, yafW gene, yeeT gene, ykfG gene, yafX gene, ykfF gene, ykfB gene, yafY gene, ypjK gene, yafZ gene, ykfA gene, perR gene, JW0245 gene, JW0246 gene, JW0247 gene, ykfC gene, JW0249 gene, is5B gene, yi51 gene, mmuP gene, mmuM gene, afuC gene, JW0255 gene, insB gene, insA gene, JW0258 gene, yagB gene, yagA gene, yagE gene, yagF gene, yagG gene, yagH gene, yagI gene, argF gene, yfjI gene, yagJ gene, yagK gene, yagL gene, yagM gene, yagN gene, intF gene, yagP gene, yagQ gene, yagR gene, yagS gene, yagT gene, yagU gene, ykgJ gene, yagV gene, yagW gene, yagX gene, yagY gene, yagZ gene, ykgK gene, ykgL gene, ykgM gene, eaeH gene, JW0292 gene, JW0293 gene, ykgA gene, ykgB gene, ykgI gene, merA gene, ykgD gene, ykgE gene, ykgF gene, ykgG gene, ykgH gene, betA gene, betB gene, betI gene, betT gene, yahA gene, yahB gene, yahC gene, yahD gene, yahE gene, yahF gene, yahG gene, yahH gene, arcM gene, yahJ gene, yahK gene, yahL gene, yahM gene, yahN gene, yahO gene, prpR gene, bcpA gene, prpC gene, prpD gene, prpE gene, codB gene, codA gene, cynR gene, cynT gene, cynS gene, cynX gene, lacA gene, lacY gene, lacZ gene, lacI gene, mhpR gene, mhpA gene, mhpB gene, mhpC gene, mhpD gene, mhpF gene, mhpE gene, mhpT gene, yaiL gene, yaiM gene, adhC gene, yaiN gene, yaiO gene, JW0350 gene, yi2A gene, yi22 gene, yi24 gene, yi23 gene, yaiP gene, yaiS gene, tauA gene, tauB gene, tauC gene, ssiD gene, yaiT gene, JW0363 gene, JW0364 gene, yaiU gene, yaiV gene, ampH gene, sbmA gene, yaiW gene, yaiY gene, yaiZ gene, ddlA gene, yaiB gene, phoA gene, psiF gene, yaiC gene, ybbO gene, tesA gene, ybbA gene, ybbP gene, rhsD gene, ybbC gene, rhsE gene, ybbD gene, JW0490 gene, ybbB gene, ybbS gene, ybbT gene, ybbU gene, ybbQ gene, ybbV gene, allP gene, allA gene, ybbY gene, ybbZ gene, ylbA gene, aliC gene, ylbC gene, fdrA gene, ylbE gene, ylbE gene, ylbE gene, ylbF gene, arcC gene, intD gene, EXO_LAMBD gene, VRPP_LAMBD gene, JW0528 gene, JW0529 gene, VREN_LAMBD gene, emrE gene, ybcK gene, ybcL gene, ybcM gene, ybcN gene, ninE gene, ybcO gene, rus gene, ybcQ gene, JW0539.5 gene, nmpC gene, vlyS gene, ybcS gene, ybcT gene, vboR gene, ybcV gene, ybcW gene, nohB gene, ybcX gene, ybcY gene, JW0552 gene, appY gene, ompT gene, envY gene, ybcH gene, nfrA gene, nfrB gene, cusS gene, cusR gene, cusC gene, ylcC gene, cusB gene, cusA gene, pheP gene, ybdG gene, nfnB gene, ybdF gene, ybdJ gene, ybdK gene, hokE gene, yi81 gene, yi82 gene, ymcC gene, ymcD gene, cspH gene, cspG gene, sfa gene, yccL gene, yccM gene, torS gene, torT gene, torR gene, torC gene, torA gene, torD gene, yccD gene, cbpA gene, yccE gene, agp gene, yccJ gene, wrbA gene, yciG gene, ycdG gene, JW0992 gene, JW0993 gene, JW0994 gene, ycdK gene, JW0996 gene, JW0997 gene, ycdC gene, putA gene, yzpU gene, putP gene, JW1002 gene, ycdO gene, ycdB gene, phoH gene, ycdP gene, ycdQ gene, ycdR gene, ycdR gene, ycdS gene, ycdT gene, JW1012 gene, JW1013 gene, JW1014 gene, ycdU gene, ymfD gene, ymfE gene, lit gene, intE gene, VXIS_BPP21 gene, ymfH gene, ymfI gene, ymfJ gene, RPC2_BPP22 gene, JW1132 gene, ymfL gene, ymfM gene, ymfN gene, ymfR gene, ymfO gene, JW1138 gene, JW1139 gene, ycfK gene, ymfS gene, ycfA gene, ycfE gene, pin gene, mcrA gene, JW1146 gene, ycgW gene, ycgX gene, ycgE gene, JW1150 gene, ycgZ gene, ymgA gene, JW1153 gene, ymgC gene, yahA gene, JW1156 gene, JW1157 gene, aidA gene, ymgD gene, JW1160 gene, JW1161 gene, JW1162 gene, ydfH gene, ydfZ gene, ydfI gene, shiA gene, JW1537 gene, tnpR gene, ydfM gene, Y314_LAMBD gene, nohA gene, ydfO gene, yccL gene, cspI gene, ydfR gene, vlyS gene, cspB gene, cspF gene, regQ_LAMBD gene, JW1552 gene, rem gene, hokD gene, relE gene, relB gene, ydfV gene, flxA gene, ydfW gene, ydfX gene, dicC gene, sdaC gene, yqeH gene, yqeI gene, yqeJ gene, yqeK gene, ygeF gene, ygeG gene, ygeH gene, ygeI gene, JW2822 gene, ygeK gene, JW2824 gene, JW2825 gene, JW2830 gene, ygeP gene, JW2832 gene, nlpD gene, yagR gene, ygeT gene, JW2836 gene, ygeV gene, ygeW gene, dpaL gene, ygeY gene, ygeZ gene, arcL gene, yqeB gene, JW2844 gene, ygfJ gene, gltD gene, JW2847 gene, ygfM gene, yagR gene, ygfO gene, ygfP gene, ygfQ gene, ygfR gene, ygfS gene, ygfT gene, pbuX gene, idi gene, epsM gene, exeL gene, exeC gene, JW2938 gene, JW2939 gene, JW2940 gene, JW2941 gene, yghK gene, glcB gene, glcG gene, glcEF gene, glcD gene, glcC gene, JW2948 gene, yghQ gene, yghR gene, yghS gene, yghT gene, yihL gene, yihM gene, yihN gene, yshA gene, yihO gene, yihP gene, yihQ gene, yihR gene, yihS gene, yihT gene, yihU gene, yihV gene, yihW gene, yihX gene, rbn gene, yihZ gene, yiiD gene, yiiE gene, yiiF gene, yiiG gene, frvR gene, frvX gene, frvB gene, frvA gene, yiiL gene, rhaD gene, rhaA gene, rhaB gene, rhaS gene, rhaR gene, rhaT gene, ptsA gene, JW3920 gene, frwC gene, frwB gene, pflD gene, pflC gene, frwD gene, yijO gene, yijP gene, JW3986 gene, yjbF gene, yjbG gene, yjbH gene, JW3989.5 gene, yjbA gene, xylE gene, maiG gene, malF gene, malE gene, malK gene, lamB gene, malM gene, yjbI gene, yjcP gene, yjcQ gene, yjcR gene, yjcS gene, alsK gene, alsE gene, alsC gene, alsA gene, alsB gene, phnQ gene, phnP gene, phnO gene, phnN gene, phnM gene, phnL gene, phnK gene, phnJ gene, phnI gene, phnH gene, phnG gene, phnF gene, phnE gene, phnD gene, phnC gene, phnB gene, phnA gene, yjdA gene, yjcZ gene, yidF gene, yidG gene, yidH gene, yidI gene, yidJ gene, yidK gene, yidL gene, JW3657 gene, glvG gene, glvB gene, glvC gene, yidP gene, yidE gene, yidQ gene, yidR gene, yidS gene, dgoT gene, dgoA gene, dgoK gene, dgoR gene, yidW gene, yidX gene, yidA gene, yidB gene, asnA gene, yieM gene, JW3724 gene, yieN gene, kup gene, rbsD gene, rbsA gene, rbsC gene, rbsB gene, rbsK gene, rbsR gene, yieO gene, yecT gene, flh gene, flhA gene, flhB gene, cheZ gene, cheY gene, cheB gene, cheR gene, tap gene, tar gene, cheW gene, cheA gene, motB gene, motA gene, flhC gene, flhD gene, JW1882 gene, JW1883 gene, yecG gene, otsA gene, otsB gene, araH gene, araG gene, araF gene, yecI gene, yecJ gene, JW1892 gene, ftn gene, yecH gene, tyrP gene, uvrC gene, uvrY gene, yecF gene, sdiA gene, yecC gene, yecS gene, yedO gene, fliY gene, fliZ gene, fliA gene, fliC gene, fliD gene, fliS gene, fliT gene, amyA gene, yedD gene, yedE gene, yedF gene, yedK gene, yedL gene, yedN gene, yedM gene, fliE gene, fliF gene, fliG gene, fliH gene, fliI gene, fliJ gene, fliK gene, fliL gene, fliM gene, fliN gene, fliO gene, flip gene, fliQ gene, fliR gene, rcsA gene, dsrB gene, JW1937 gene, yedA gene, vsr gene, dcm gene, yedJ gene, JW1946 gene, ompC gene, JW1948 gene, yedS gene, yedU gene, yedV gene, copR gene, yedX gene, JW1954 gene, JW1955 gene, JW1956 gene, JW1974 gene, JW1979 gene, JW1980 gene, yeeP gene, flu gene, JW1983 gene, yeeS gene, yeeT gene, yeeU gene, yeeV gene, yeeW gene, yefJ gene, JW2013 gene, yefJ gene, yefI gene, wbbJ gene, yefG gene, rfc gene, glf gene, rfbX gene, rfbC gene, rfbA gene, rfbD gene, rfbB gene, intA gene, yfjH gene, alpA gene, yfjI gene, JW2606 gene, yfjJ gene, yfjK gene, yfjL gene, yfjM gene, yfjN gene, yfjO gene, yfjP gene, yfjQ gene, yfjR gene, ypjK gene, yfjS gene, yfjT gene, yfjU gene, yfjV gene, JW2622 gene, yfjW gene, JW2623.5 gene, yfjX gene, yfjY gene, yfjZ gene, ypjF gene, ypjA gene, JW2629 gene, JW2630 gene, JW2631 gene, JW2632 gene, JW2633 gene, JW2634 gene, ygaE gene, yzzM gene, yqaE gene, JW2642 gene, ygaP gene, stpA gene, JW2645 gene, ygaC gene, ygaM gene, nrdH gene, nrdI gene, nrdE gene, proV gene, proW gene, proX gene, JW2655 gene, JW2656 gene, ygaZ gene, ygaH gene, srlA gene, srlB gene, srlD gene, gutM gene, srlR gene, gutQ gene, gltF gene, yhcA gene, yhcD gene, yhcE gene, yhcE gene, yhcF gene, yhcG gene, yhcH gene, nanK gene, nanE gene, yegE gene, alkA gene, yegD gene, JW2055 gene, JW2056 gene, JW2057 gene, JW2058 gene, yegM gene, yegN gene, yegO gene, yegB gene, baeS gene, baeR gene, JW2065 gene, yegQ gene, ogrK gene, JW2068 gene, JW2069 gene, yegS gene, gatR gene, JW2072 gene, JW2073 gene, gatR gene, gatD gene, gatC gene, gatB gene, gatA gene, gatZ gene, gatY gene, fbaB gene, xegT gene, yegU gene, yegV gene, yegW gene, yegX gene, molR gene, yehI gene, yehK gene, yehL gene, yehO gene, yehP gene, yehQ gene, yehR gene, yehS gene, yehT gene, yehU gene, yehV gene, yehW gene, yehX gene, yehY gene, yehZ gene, bglX gene, dld gene, pbpG gene, yohC gene, yeiI gene, yeiJ gene, yeiK gene, yeiL gene, yeiM gene, yeiN gene, yeiC gene, cmtA gene, cmtB gene, intB gene, JW4228 gene, yjgW gene, yjgX gene, yjgY gene, yjgZ gene, yi41 gene, yi42 gene, yjhB gene, yjhC gene, yjhD gene, yjhE gene, yi91 gene, JW4244 gene, JW4245 gene, JW4246 gene, yjhU gene, yjhF gene, yjhG gene, yjhH gene, yjhI gene, sgcR gene, sgcE gene, sgcA gene, sgcQ gene, sgcC gene, JW4266.5 gene, sgcX gene, yjhP gene, yjhQ gene, JW4269.5 gene, JW4270 gene, JW4270.5 gene, yjhR gene, yjhS gene, yjhT gene, yjhA gene, fimB gene, fimE gene, fimA gene, fimI gene, fimC gene, fimD gene, fimF gene, fimG gene, fimH gene, gntP gene, uxuA gene, uxuB gene, uxuR gene, yjiC gene, yjiD gene, yjiE gene, iadA gene, yjiG gene, yjiH gene, yjiI gene, yjiJ gene, yjiK gene, JW4296.5 gene, yjiL gene, yjiM gene, yjiN gene, yjiO gene, yjiP gene, yjiQ gene, yjiR gene, yjiS gene, yjiT gene, yjiV gene, mcrD gene, mcrC gene, mcrB gene, yjiW gene, hsdS gene, hsdM gene, hsdR gene, mrr gene, yjiA gene, yjiX gene, yjiY gene, tsr gene, yjjL gene, JW4320 gene, yjjM gene, yjjN gene, mdoB gene, yjjA gene, kdpF gene, ybfA gene, rhsC gene, JW0690 gene, ybfB gene, rhsA gene, ybfC gene, ybfL gene, ybfD gene, ybgA gene, yjfI gene, yjfJ gene, yjfK gene, yjfL gene, yjfM gene, yjfC gene, aidB gene, yjfN gene, yjfO gene, yjfP gene, yjfQ gene, yjfR gene, sgaT gene, sgaB gene, sgaA gene, sgaH gene, sgaU gene, rhsB gene, JW3448 gene, yhhH gene, yibA gene, yhhI gene, yhhJ gene, yhiH gene, yhiI gene, yhiJ gene, yhiK gene, yhiL gene, yhiM gene, yhiN gene, JW0782 gene, ybiA gene, dinG gene, ybiB gene, ybiC gene, ybiJ gene, ybiI gene, ybiX gene, ybiL gene, ybiM gene, ybiN gene, ybiO gene, glnQ gene, glnP gene, glnH gene, yliA gene, yliB gene, yliC gene, yliD gene, JW0817 gene, JW0818 gene, yliG gene, yliH gene, yliI gene, yliJ gene, dacC gene, deoR gene, ybjG gene, ybjC gene, mdaA gene, rimK gene, ybjN gene, potF gene, potG gene, potH gene, potI gene, ybjO gene, ybjF gene, artJ gene, artM gene, artQ gene, artI gene, artP gene, ybjP gene, JW0850 gene, JW0851 gene, JW0852 gene, ybjT gene and ltaA gene, or homologous gene thereof.

(26) The E. coli mutant strain according to any one of (23) to (25) comprising chromosomal DNA that does not comprise DNA having the nucleotide sequences as shown in [1] to [43] of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.

• [1] a nucleotide sequence of nucleotides 243965 to 253410
• [2] a nucleotide sequence of nucleotides 261964 to 387531
• [3] a nucleotide sequence of nucleotides 389139 to 403703
• [4] a nucleotide sequence of nucleotides 518030 to 532711
• [5] a nucleotide sequence of nucleotides 535505 to 550215
• [6] a nucleotide sequence of nucleotides 563941 to 608118
• [7] a nucleotide sequence of nucleotides 728821 to 739593
• [8] a nucleotide sequence of nucleotides 832552 to 848089
• [9] a nucleotide sequence of nucleotides 867638 to 883473
• [10] a nucleotide sequence of nucleotides 890998 to 909379
• [11] a nucleotide sequence of nucleotides 1048888 to 1097019
• [12] a nucleotide sequence of nucleotides 1198106 to 1225146
• [13] a nucleotide sequence of nucleotides 1630912 to 1641038
• [14] a nucleotide sequence of nucleotides 1642632 to 1650222
• [15] a nucleotide sequence of nucleotides 1964524 to 1993075
• [16] a nucleotide sequence of nucleotides 1996662 to 2028209
• [17] a nucleotide sequence of nucleotides 2033449 to 2044376
• [18] a nucleotide sequence of nucleotides 2069149 to 2081926
• [19] a nucleotide sequence of nucleotides 2104393 to 2116064
• [20] a nucleotide sequence of nucleotides 2146264 to 2187855
• [21] a nucleotide sequence of nucleotides 2200669 to 2229850
• [22] a nucleotide sequence of nucleotides 2255895 to 2263491
• [23] a nucleotide sequence of nucleotides 2755677 to 2789480
• [24] a nucleotide sequence of nucleotides 2795174 to 2800890
• [25] a nucleotide sequence of nucleotides 2804308 to 2810147
• [26] a nucleotide sequence of nucleotides 2825314 to 2830262
• [27] a nucleotide sequence of nucleotides 2985362 to 3033128
• [28] a nucleotide sequence of nucleotides 3077085 to 3078916
• [29] a nucleotide sequence of nucleotides 3110110 to 3134341
• [30] a nucleotide sequence of nucleotides 3360737 to 3370547
• [31] a nucleotide sequence of nucleotides 3618576 to 3636916
• [32] a nucleotide sequence of nucleotides 3854662 to 3865831
• [33] a nucleotide sequence of nucleotides 3867102 to 3877010
• [34] a nucleotide sequence of nucleotides 3926659 to 3938878
• [35] a nucleotide sequence of nucleotides 4059898 to 4079659
• [36] a nucleotide sequence of nucleotides 4086451 to 4099982
• [37] a nucleotide sequence of nucleotides 4139232 to 4149709
• [38] a nucleotide sequence of nucleotides 4235359 to 4251735
• [39] a nucleotide sequence of nucleotides 4298977 to 4311511
• [40] a nucleotide sequence of nucleotides 4313318 to 4329721
• [41] a nucleotide sequence of nucleotides 4409533 to 4423151
• [42] a nucleotide sequence of nucleotides 4496202 to 4509534
• [43] a nucleotide sequence of nucleotides 4517876 to 4599637

(27) The E. coli mutant strain according to any one of (23) to (26) comprising chromosomal DNA that does not comprise ygiS gene, ygiT gene, ygiU gene, JW2991 gene, ygiW gene, ygiX gene, ygiY gene, ygiZ gene, mdaB gene and ygiN gene, or homologous gene thereof.

(28) The E. coli mutant strain according to any one of (23) to (27) comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 3165628 to 3172940 of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.

(29) The E. coli mutant strain according to any one of (23) to (28) comprising chromosomal DNA that does not comprise ybeL gene, ybeQ gene, ybeR gene, ybeS gene, ybeT gene, ybeU gene, ybeV gene, ybeW gene, ybeK gene, gltL gene, gltK gene, gltJ gene, ybeJ gene and JW0651 gene, or homologous gene thereof.

(30) The E. coli mutant strain according to any one of (23) to (29) comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 675104 to 689374 of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.

(31) The E. coli mutant strain according to any one of (23) to (30) comprising chromosomal DNA that does not comprise yfcC gene, JW2296 gene, yfcE gene, yfcF gene, yfcG gene, folX gene, JW2301 gene, yfcI gene, hisP gene, hisM gene, hisQ gene, hisJ gene, argT gene, yfdB gene, JW2346 gene, yfdH gene, JW2348 gene, yfdK gene, yfdL gene, yfdM gene, xylU gene, yfdN gene, yfdO gene, JW2356 gene, JW2357 gene, JW2358 gene, JW2359 gene, JW2360 gene, JW2378 gene, yqhT gene, ptwC gene andptwB gene, or homologous gene thereof.

(32) The E. coli mutant strain according to any one of (23) to (31) comprising chromosomal DNA that does not comprise DNA having the nucleotide sequences as shown in [1] to [3] below of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.

• [1] a nucleotide sequence of nucleotides 2423365 to 2434098
• [2] a nucleotide sequence of nucleotides 2472852 to 2482482
• [3] a nucleotide sequence of nucleotides 2505075 to 2514557

(33) The E. coli mutant strain according to any one of (23) to (32) comprising chromosomal DNA that does not comprise pinO gene, gspA gene, gspC gene, gspD gene, gspE gene, hofF gene, hofG gene, hofH gene, gspI gene, gspJ gene, gspK gene, gspL gene, pshM gene, hofD gene, bfr gene, yheA gene and chiA gene, or homologous gene thereof.

(34) The E. coli mutant strain according to any one of (23) to (33) comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 3453021 to 3469366 of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.

(35) The E. coli mutant strain according to any one of (23) to (34) comprising chromosomal DNA that does not comprise elaB gene, elaA gene, elaC gene, elaD gene, yfbK gene, yfbL gene, yfbM gene, yfbN gene, JW2269 gene and JW2270 gene, or homologous gene thereof.

(36) The E. coli mutant strain according to any one of (23) to (35) comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 2386262 to 2395471 of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.

(37) The E. coli mutant strain according to any one of (23) to (36) comprising chromosomal DNA that does not comprise yhdJ gene, yhdU gene, envR gene, acrE gene, envD gene, yhdV gene, yhdW gene, yhdX gene and yhdY gene, or homologous gene thereof.

(38) The E. coli mutant strain according to any one of (23) to (37) comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 3411160 to 3420878 of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.

(39) The E. Coli mutant strain according to any one of (23) to (38) comprising chromosomal DNA that does not comprise chpA gene or homologous gene thereof.

(40) The E. coli mutant strain according to any one of (23) to (39) comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 2910274 to 2910573 of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.

(41) The E. coli mutant strain according to any one of (23) to (40) comprising chromosomal DNA that does not comprise fhuA gene, fhuC gene, fhuD gene fhuB gene, or homologous gene thereof.

(42) The E. coli mutant strain according to any one of (23) to (41) comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 167065 to 172979 of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.

(43) The E. coli mutant strain according to any one of (1) to (42) obtained via modification by a method selected from among the following [1] to [5]:

• [1] a method for reducing or removing at least one mechanism for regulating biosynthesis of the useful substance;
• [2] a method for increasing the expression level of at least one enzyme associated with biosynthesis of the useful substance;
• [3] a method for increasing the copy number of at least one enzyme gene associated with biosynthesis of the useful substance;
• [4] a method for attenuating or blocking at least one metabolic pathway which branches from the biosynthetic pathway of the useful substance into metabolites other than the useful substance; or
• [5] a method for selecting a strain that exhibits higher resistance to an analogue of a useful substance than a wild-type E. coli strain.

(44) The E. coli mutant strain according to any one of (1) to (43), wherein the E. coli strain is the E. coli K-12, B, or W strain.

(45) The E. coli mutant strain according to (44), wherein the E. coli K-12 strain is the E. coli W3110 strain (ATCC27325) or the E. coli MG1655 strain (ATCC47076).

(46) A method for producing a useful substance comprising culturing an E. coli mutant strain according to any one of (1) to (45) in a medium so as to generate and accumulate such useful substance in the culture and recovering the useful substance from the culture

(47) The method according to any one of (43) to (46), wherein the useful substance is selected from the group consisting of proteins, peptides, amino acids, nucleic acids, vitamins, saccharides, organic acids, and lipids.

Effects of the Invention

The present invention can provide an E. coli mutant, the amount of cells of which, after a given period of culture, is larger than that of a wild-type E. coli strain, and a process for producing a useful substance using such mutant.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows the structure of a DNA fragment comprising sacB gene and cat gene ligated thereto.

DESCRIPTION OF REFERENCE SYMBOLS

sacB represents the sacB gene, and cat represents the cat gene.

BEST MODES FOR CARRYING OUT THE INVENTION

Wild-type E. coli strains may be any E. coli strains that are found in natural populations at the highest frequency. Examples thereof include the E. coli W3110 strain (ATCC27325) and the E. coli MG1655 strain (ATCC47076) that belong to the E. coli K-12 strain, the ATCC11303 strain that belongs to the E. coli B strain, and the ATCC9637 strain that belongs to the E. coli W strain.

Table 1, Table 2, and SEQ ID NO: 1 of the present description and the Internet site URL::http://gib.genes.nig.ac.jp/single/index.php?spid=Ecol_K12_W3110 include nucleotide sequences of the genes identified with JW numbers. The numbers with “JW” represent gene numbers registered at the DNA Data Bank of Japan.

The nucleotide sequence as shown in SEQ ID NO: 1 is the nucleotide sequence of chromosomal DNA of the E. coli W3110 strain registered as of Oct. 29, 2004 on the above Internet site.

The term “chromosomal DNA that contains genes” refers to chromosomal DNA that contains a structural gene and a region containing a specific regulatory function such as a prmoter, operator or the like of a given gene.

The term “chromosomal DNA that does not contain gene” refers to chromosomal DNA that does not contain a part of or an entire structural gene of a given gene or chromosomal DNA that does not contain a region having a specific regulatory function, such as a promoter and/or operator of such gene.

The “chromosomal DNA that does not contain part of a structural gene” may be any chromosomal DNA that does not contain part of a structural gene, and a functional protein is not transcribed and expressed from the structural gene. Examples thereof include DNA that has no initiation codon due to a lack of the 5′ end region of the structural gene and chromosomal DNA that lacks most of the 3′ end region, such as at least 10%, preferably at least 20%, more preferably at least 30%, and further preferably at least 40% of the entire 3′ end region. Chromosomal DNA that does not contain two adjacent genes may be chromosomal DNA that does not contain DNA comprising a nucleotide sequence located between such genes.

The term “homologous genes” refers to genes that have homology in terms of the nucleotide sequences of such genes or genes encoding the proteins which have homology in terms of amino acid sequences among E. coli species. Having homology refers to having similarity of at least 85%, preferably at least 90%, more preferably at least 95%, farther preferably at least 98%, and particularly preferably at least 99%, in the case of nucleotide sequences. In the case of amino acid sequences, such term refers to having similarity of at least 80%, preferably at least 90%, more preferably at least 95%, farther preferably at least 98%, and particularly preferably at least 99%.

“DNA equivalent to DNA having a nucleotide sequence represented by given nucleotide numbers in the nucleotide sequence as shown in SEQ ID NO: 1” refers to DNA that is substantially the same as DNA having a nucleotide sequence represented by such given nucleotide numbers of an E. coli species in terms of the structure of the gene contained and the position on chromosomal DNA. When structures of genes contained in DNA are substantially the same as each other, genes contained in such DNA are identical or homologous to each other, and arranged in the same order. In that case, nucleotide sequences may differ among genes. When the DNA having a nucleotide sequence represented by given nucleotide numbers contains an insertion sequence, however, such insertion sequence may be absent in DNA equivalent to the aforementioned DNA. “Substantially the same positions on chromosomal DNA” refers to the following conditions. When adenine of the initiation codon of the ThrA gene on E. coli chromosomal DNA is designated as No. 1 and the nucleotide sequence of chromosomal DNA is numbered along the coding chain of the ThrA gene, differences between such nucleotide numbers and the given nucleotide numbers are 50 or smaller, preferably 30 or smaller, more preferably 10 or smaller, and further preferably 5 or smaller. When the DNA having a nucleotide sequence represented by given nucleotide numbers contains an insertion sequence, however, DNA equivalent to the aforementioned DNA does not necessarily contain such insertion sequence. Thus, such insertion sequence and nucleotide numbers may be different. An example of DNA equivalent to DNA having a nucleotide sequence represented by given nucleotide numbers of the nucleotide sequence as shown in SEQ ID NO: 1 is DNA having sequence similarity of at least 80%, preferably at least 90%, more preferably at least 95%, further preferably at least 98%, and particularly preferably at least 99% with the nucleotide sequence represented by given nucleotide numbers.

Amino acid sequence or nucleotide sequence homology can be determined with the utilization of the BLAST algorithm of Karlin and Altschul (Pro. Natl. Acad. Sci., U.S.A., 90 5873, 1993) or the FASTA algorithm (Methods Enzymol., 183, 63, 1990). Based on the BLAST algorithm, programs referred to as BLASTN or BLASTX have been developed (J. Mol. Biol., 215, 403, 1990). When analyzing nucleotide sequences by BLASTN based on BLAST, the score parameter is set to 100 and the word length parameter is set to 12, for example. When analyzing amino acid sequences via BLASTX based on BLAST, the score parameter is set to 50 and the word length parameter is set to 3, for example. When using BLAST and Gapped BLAST programs, the default parameters thereof are used. Specific procedures of such analytical methods are known (http://www.ncbi.nlm.nih.gov.).

1. E. coli Mutant Strain of the Present Invention

The E. coli mutant strain of the present invention may be any E. coli mutant strain comprising chromosomal DNA that does not comprise any gene excluding the genes [1] and

that are present on chromosomal DNA of a wild-type E. coli strain or homologous genes thereof, provided that it comprises the following genes [1] and [2] or homologous genes thereof and having chromosomal DNA that is at least 470 kbp shorter than that of a wild-type E. coli strain,

[1] thrA gene, thrB gene, thrc gene, talB gene, mog gene, htgA gene, dnaK gene, dnaJ gene, hokC gene, nhaA gene, nhaR gene, rpsT gene, ribF gene, ileS gene, lspA gene, fkpB gene, lytB gene, dapB gene, carA gene, carB gene, caiF gene, caiE gene, caiD gene, caiC gene, caiB gene, caiA gene, caiT gene, fixA gene, fixB gene, fixC gene, fixX gene, kefC gene, folA gene, apaH gene, apaG gene, ksgA gene, pdxA gene, surA gene, imp gene, djlA gene, rluA gene, hepA gene, polB gene, araD gene, araA gene, araB gene, araC gene, tbpA gene, leuD gene, leuC gene, leuB gene, leuA gene, leuL gene, leuO gene, ilvI gene, ilvH gene, fruR gene, ftsL gene, ftsI gene, murE gene, murF gene, mraY gene, murD gene, ftsW gene, murG gene, murC gene, ddlB gene, ftsQ gene, ftsA gene, ftsZ gene, lpxC gene, secA gene, mutT gene, yacE gene, guaC gene, hofC gene, hofB gene, ppdD gene, nadC gene, ampD gene, ampE gene, aroP gene, pdhR gene, aceE gene, aceF gene, lpdA gene, acnB gene, speD gene, speE gene, gcd gene, hpt gene, yadF gene, panD gene, panC gene, panB gene, htrE gene, ecpD gene, folK gene, pcnB gene, dksA gene, sfsA gene, ligT gene, hrpB gene, ponB gene, hemL gene, pfs gene, dgt gene, htrA gene, dapD gene, glnD gene, map gene, rpsB gene, tsf gene, smb gene, rrf gene, dxr gene, rth gene, cdsA gene, yaeL gene, hlpA gene, lpxD gene, fabZ gene, lpxA gene, lpxB gene, rnhB gene, dnaE gene, accA gene, ldcC gene, mesJ gene, rof gene, cutF gene, proS gene, rcsF gene, abc gene, dniR gene, gloB gene, mhA gene, dnaQ gene, up18 gene, gmhA gene, pepD gene, gpt gene, crl gene, phoE gene, proB gene, proA gene, hemB gene, proC gene, aroL gene, aroM gene, araJ gene, sbcC gene, sbcD gene, phoB gene, phoR gene, brnQ gene, proY gene, malZ gene, queA gene, tgt gene, secD gene, secF gene, tsx gene, ribD gene, ribH gene, nusB gene, thiL gene, pgpA gene, dxs gene, ispA gene, xseB gene, thiI gene, thiJ gene, apbA gene, cyoE gene, cyoD gene, cyoC gene, cyoB gene, cyoA gene, ampG gene, bolA gene, tig gene, clpP gene, clpX gene, lon gene, hupB gene, cof gene, mdlA gene, mdlB gene, glnK gene, amtB gene, tesB gene, maa gene, hha gene, acrB gene, acrA gene, acrR gene, aefA gene, priC gene, apt gene, dnaX gene, recR gene, htpG gene, adk gene, hemH gene, aes gene, gsk gene, fsr gene, ushA gene, cueR gene, gel gene, gip gene, purK gene, purE gene, ybbF gene, ppiB gene, cysS gene, folD gene, sfmA gene, sfmC gene, sfmD gene, sfmH gene, sfmF gene, fimZ gene, entD gene, fepA gene, fes gene, entF gene, fepE gene, fepC gene, fepG gene, fepD gene, fepB gene, entC gene, entE gene, entB gene, entA gene, cstA gene, dsbG gene, ahpC gene, ahpF gene, mk gene, ma gene, citT gene, citG gene, citX gene, citF gene, citE gene, citD gene, citC gene, citA gene, citB gene, dcuC gene, dcuC gene, crcA gene, cspE gene, crcB gene, lipA gene, lipB gene, dacA gene, rlpA gene, mrdB gene, mrdA gene, phpB gene, nadD gene, holA gene, rlpB gene, leuS gene, lnt gene, phoL gene, ubiF gene, asnB gene, nagD gene, nagC gene, nagA gene, nagB gene, nagE gene, glnS gene, fur gene, fldA gene, seqA gene, pgm gene, potE gene, speF gene, kdpE gene, kdpD gene, kdpC gene, kdpB gene, kdpA gene, phrB gene, end8 gene, abrB gene, gltA gene, sdhC gene, sdhD gene, sdhA gene, sdhB gene, sucA gene, sucB gene, sucC gene, sucD gene, farR gene, hrsA gene, cydA gene, cydB gene, tolQ gene, toIR gene, tolA gene, tolB gene, pal gene, nadA gene, pnuC gene, czcD gene, aroG gene, gpmA gene, galM gene, galK gene, galT gene, galE gene, modF gene, modE gene, modA gene, modB gene, modC gene, bioA gene, bioB gene, bioF gene, bioC gene, bioD gene, uvrB gene, moaA gene, moaB gene, moaC gene, moaD gene, moaE gene, rhlE gene, dps gene, ompX gene, mipB gene, moeB gene, moeA gene, mdfA gene, grxA gene, poxB gene, aqpZ gene, cspD gene, clpA gene, infA gene, aat gene, cydC gene, cydD gene, trxB gene, lrp gene, ftsK gene, lolA gene, serS gene, dmsA gene, dmsB gene, dmsC gene, pflA gene, pflB gene, focA gene, serC gene, aroA gene, cmk gene, rpsA gene, himD gene, msbA gene, lpxK gene, kdsB gene, smtA gene, mukF gene, kicA gene, mukB gene, aspC gene, ompF gene, asnS gene, pncB gene, pepN gene, ssuB gene, ssuC gene, ssuD gene, ssuA gene, ssuE gene, pyrD gene, uup gene, pqiA gene, pqiB gene, rmf gene, fabA gene, ompA gene, sulA gene, helD gene, mgsA gene, acyP gene, hyaA gene, hyaB gene, hyaC gene, hyaD gene, hyaE gene, hyaF gene, appC gene, appB gene, appA gene, csgG gene, csgF gene, csgE gene, csgD gene, csgB gene, csgA gene, csgC gene, sfaA gene, mdoG gene, mdoH gene, msyB gene, htrB gene, solA gene, dinI gene, pyrC gene, grxB gene, rimJ gene, mviM gene, mviN gene, flgN gene, flgM gene, flgA gene, flgB gene, flgC gene, flgD gene, flgE gene, flgF gene, flgG gene, flgH gene, flgI gene, flgJ gene, flgK gene, flgL gene, rne gene, rluC gene, rpmF gene, plsX gene, fabH gene, fabD gene, fabG gene, acpP gene, fabF gene, pabC gene, tmik gene, holB gene, ptsG gene, fhuE gene, nagZ gene, ndh gene, mfd gene, ycfU gene, ycfV gene, ycfW gene, cobB gene, potD gene, potC gene, potB gene, potA gene, pepT gene, phoQ gene, phoP gene, purB gene, trmU gene, icdA gene, minE gene, minD gene, minC gene, clyA gene, umuD gene, umuC gene, dsbB gene, nhaB gene, fadR gene, dadA gene, dadX gene, ldcA gene, mltE gene, tagl gene, treA gene, dhaR gene, pth gene, prsA gene, ychB gene, hemM gene, hemA gene, prfA gene, hemK gene, kdsA gene, chaA gene, chaB gene, chaC gene, narL gene, narX gene, narK gene, narG gene, narH gene, narJ gene, narI gene, tpr gene, purU gene, sprE gene, galU gene, hns gene, tdk gene, adhE gene, oppA gene, oppB gene, oppC gene, oppD gene, oppF gene, cls gene, kch gene, tonB gene, trpA gene, trpB gene, trpC gene, trpD gene, trpE gene, trpL gene, btuR gene, sohB gene, topA gene, cysB gene, acnA gene, ribA gene, pgpB gene, pyrF gene, osmB gene, rnb gene, envM gene, sapF gene, sapD gene, sapC gene, sapB gene, sapA gene, aldH gene, ordL gene, goaG gene, pspF gene, pspA gene, pspB gene, pspC gene, pspD gene, pspE gene, sucP gene, ompG gene, tyrR gene, tpx gene, mppA gene, fnr gene, ogt gene, dbpA gene, intR gene, lar gene, recT gene, recE gene, racC gene, kil gene, sieB gene, racR gene, dnaC gene, enpP gene, trkG gene, VMTH_LAMBD gene, VLOM_LAMBD gene, tnpO gene, upo3 gene, ompN gene, nifJ gene, hslJ gene, ldhA gene, feaR gene, feaB gene, tynA gene, paaZ gene, paaA gene, paaB gene, paaC gene, paaD gene, paaE gene, paaF gene, paaG gene, paaH gene, paaI gene, paaJ gene, paaK gene, paaX gene, paaY gene, acpD gene, hrpA gene, aldA gene, gapC gene, gapC gene, cybB gene, trg gene, rimL gene, tehA gene, tehB gene, prtC gene, potA gene, potC gene, ansP gene, nhoA gene, narV gene, narW gene, narY gene, narZ gene, narU gene, fdnG gene, fdnH gene, fdnI gene, adhP gene, sfcA gene, rpsV gene, osmC gene, dos gene, xasA gene, gadB gene, pqqL gene, chuR gene, hipA gene, hipB gene, tam gene, uxaB gene, marC gene, marR gene, marA gene, marB gene, dcp gene, lycV gene, dicA gene, dicB gene, rspB gene, rspA gene, speG gene, dmsB gene, dmsC gene, bioD gene, mlc gene, asr gene, ebr gene, pntB gene, pntA gene, arcD gene, rstA gene, rstB gene, tus gene, fumC gene, fumA gene, manA gene, uidC gene, uidB gene, uidA gene, uidR gene, hdhA gene, malI gene, malX gene, malY gene, add gene, blr gene, nth gene, gst gene, pdxY gene, tyrS gene, pdxH gene, slyB gene, slyA gene, sodC gene, nemA gene, gloA gene, rnt gene, lhr gene, sodB gene, araJ gene, purR gene, cfa gene, ribE gene, ag43 gene, phsC gene, nrfC gene, pykF gene, lpp gene, sufS gene, aroD gene, ppsA gene, aroH gene, nlpC gene, btuD gene, btuE gene, btuC gene, himA gene, pheT gene, pheS gene, pheM gene, rplT gene, rpmI gene, infC gene, thrS gene, arp gene, pfkB gene, cedA gene, katE gene, celF gene, celD gene, celC gene, celB gene, celA gene, osmE gene, nadE gene, spy gene, astE gene, astB gene, astD gene, astA gene, cstC gene, xthA gene, gdhA gene, topB gene, selD gene, sppA gene, ansA gene, tdh gene, gapA gene, ttuC gene, caiT gene, rnd gene, fadD gene, slp gene, pabB gene, sdaA gene, manX gene, manY gene, manZ gene, rrmA gene, cspC gene, kdgR gene, tcmA gene, htpX gene, prc gene, pqiB gene, pphA gene, holE gene, exoX gene, ptrB gene, purT gene, eda gene, edd gene, zwf gene, pykA gene, mlt gene, znuA gene, znuC gene, ruvB gene, ruvA gene, ruvC gene, ntpA gene, aspS gene, bisZ gene, torY gene, cutC gene, argS gene, pgsA gene, amn gene, cbl gene, nac gene, erfK gene, cobT gene, cobS gene, cobU gene, gyrI gene, phsE gene, sbcB gene, hisL gene, hisG gene, hisD gene, hisC gene, hisB gene, hisH gene, hisA gene, hisF gene, hisI gene, wzz gene, ugd gene, gnd gene, galF gene, wcaM gene, wcaL gene, wcaK gene, wzxC gene, wcaJ gene, cpsG gene, cpsB gene, wcal gene, wcaH gene, wcaG gene, gmd gene, wcaF gene, wcaE gene, wcaD gene, wcaC gene, wcaB gene, wcaA gene, wzb gene, wza gene, asmA gene, dcd gene, udk gene, thiD gene, thiM gene, mrp gene, metG gene, cdd gene, sanA gene, mglC gene, mglA gene, mglB gene, galS gene, folE gene, cirA gene, lysP gene, nfo gene, fruA gene, fruK gene, fruB gene, rplY gene, narP gene, ccmH gene, dsbE gene, ccmF gene, ccmE gene, ccmD gene, ccmC gene, ccmB gene, ccmA gene, napC gene, napB gene, napH gene, napG gene, napA gene, napD gene, napF gene, eco gene, alkB gene, ada gene, apbE gene, rcsB gene, rcsC gene, atoS gene, atoC gene, atoD gene, atoA gene, atoE gene, atoB gene, gyrA gene, ubiG gene, nrdA gene, nrdB gene, inaA gene, glpQ gene, glpT gene, glpA gene, glpB gene, glpC gene, cinA gene, ais gene, pmrD gene, menE gene, menC gene, menB gene, menD gene, menF gene, nuoN gene, nuoM gene, nuoL gene, nuoK gene, nuoJ gene, nuoI gene, nuoH gene, nuoG gene, nuoF gene, nuoE gene, nuoD gene, nuoB gene, nuoA gene, lrhA gene, ackA gene, pta gene, ubiX gene, purF gene, dedE gene, dedD gene, folC gene, accD gene, dedA gene, truA gene, usg1 gene, pdxB gene, div gene, fabB gene, ddpX gene, mepA gene, aroC gene, sixA gene, fadL gene, vacJ gene, dsdC gene, dsdX gene, dsdA gene, emrY gene, emrK gene, evgA gene, evgS gene, bifF gene, ddg gene, glk gene, mntH gene, nupC gene, gltX gene, xapR gene, xapB gene, xapA gene, lig gene, zipA gene, cysZ gene, cysK gene, ptsH gene, ptsI gene, gsr gene, pdxK gene, cysM gene, cysA gene, cysW gene, cysU gene, cysP gene, ucpA gene, ptbA gene, amiA gene, hemF gene, cchA gene, eutC gene, eutB gene, eutH gene, eutG gene, eutJ gene, eutE gene, cchB gene, eutI gene, talA gene, tktB gene, aegA gene, narQ gene, acrD gene, dapE gene, purC gene, nlpB gene, dapA gene, gcvR gene, bcp gene, hyfA gene, hyfB gene, hyfC gene, hyfD gene, hyffE gene, hyfF gene, hyfG gene, hyfH gene, hyfI gene, hyfJ gene, hyfR gene, focB gene, perM gene, yfgE gene, uraA gene, upp gene, purM gene, purN gene, ppk gene, ppx gene, guaA gene, guaB gene, xseA gene, hisS gene, gcpE gene, ndk gene, pbpC gene, sseA gene, sseB gene, pepB gene, fdx gene, hscA gene, hscB gene, iscU gene, iscS gene, iscR gene, suhB gene, csiE gene, dgxA gene, dgxB gene, hcaC gene, hcaB gene, hcaD gene, glyA gene, hmpA gene, glnB gene, purL gene, acpS gene, pdxJ gene, recO gene, era gene, me gene, lepB gene, lepA gene, rseC gene, rseB gene, rseA gene, rpoE gene, nadB gene, srmB gene, ung gene, trx2 gene, pssA gene, kgtP gene, clpB gene, rluD gene, pheL gene, pheA gene, tyrA gene, aroF gene, rplS gene, trmD gene, rimM gene, rpsP gene, ffh gene, grpE gene, recN gene, smpA gene, smpB gene, gabD gene, gabT gene, gabP gene, nrdF gene, emrR gene, emrA gene, emrB gene, gshA gene, csrA gene, alaS gene, oraA gene, recA gene, mltB gene, hypF gene, hydN gene, ascG gene, ascF gene, ascB gene, hycI gene, hycH gene, hycG gene, hycF gene, hycE gene, hycD gene, hycC gene, hycB gene, hycA gene, hypA gene, hypB gene, hypC gene, hypD gene, hypE gene, fhlA gene, mutS gene, prpB gene, rpoS gene, pcm gene, surE gene, ygbB gene, ygbP gene, ygbQ gene, cysC gene, cysN gene, cysD gene, iap gene, cysH gene, cysI gene, cysJ gene, ptpS gene, eno gene, pyrG gene, mazG gene, chpR gene, relA gene, barA gene, gud2 gene, gudT gene, mioC gene, syd gene, sdaB gene, exo gene, fucO gene, fucA gene, fucP gene, fucI gene, fucK gene, fucU gene, fucR gene, gcvA gene, nifS gene, mltA gene, amiC gene, argA gene, thyA gene, lgt gene, ptsP gene, mutH gene, tas gene, aas gene, galR gene, lysA gene, lysR gene, araE gene, kduD gene, kduI gene, lysS gene, prfB gene, recJ gene, dsbC gene, xerD gene, fldB gene, up14 gene, bglA gene, gcvP gene, gevH gene, gcvT gene, visC gene, ubiH gene, pepP gene, serA gene, rpiA gene, iciA gene, sbm gene, argK gene, fba gene, pgk gene, epd gene, tktA gene, speB gene, speA gene, metK gene, galP gene, sprT gene, endA gene, gshB gene, yqgF gene, ansB gene, mutY gene, mltC gene, nupG gene, speC gene, pitB gene, gsp gene, hybG gene, hybF gene, hybE gene, hybD gene, hybC gene, hybB gene, hybA gene, hoxK gene, exbD gene, exbB gene, metC gene, exsA gene, sufl gene, plsC gene, parC gene, parE gene, icc gene, tolC gene, ribB gene, glgS gene, rfaE gene, glnE gene, cca gene, bacA gene, folB gene, ttdA gene, ttdB gene, ygjD gene, rpsU gene, dnaG gene, rpoD gene, aer gene, ebgR gene, ebgA gene, ebgC gene, fadH gene, uxaA gene, uxaC gene, exuT gene, exuR gene, tdcE gene, tdcD gene, tdcC gene, tdcB gene, tdcA gene, tdcR gene, sohA gene, agaR gene, agaZ gene, agaV gene, agaW gene, agaA gene, agaS gene, agaY gene, agaB gene, agaC gene, agaD gene, agal gene, mtr gene, deaD gene, nlpI gene, pnp gene, rpsO gene, truB gene, rbfA gene, infB gene, nusA gene, argG gene, secG gene, mrsA gene, folP gene, ftsH gene, ftsJ gene, greA gene, dacB gene, yhbZ gene, rpmA gene, rplU gene, ispB gene, nlp gene, murA gene, yrbI gene, yrbK gene, rpoN gene, ptsN gene, ptsO gene, mtgA gene, arcB gene, gltB gene, nanT gene, npl gene, nanR gene, dcuD gene, sspB gene, sspA gene, rpsI gene, rpiM gene, hhoA gene, hhoB gene, mdh gene, argR gene, tldD gene, rmg gene, mreD gene, mreC gene, mreB gene, accB gene, accC gene, panF gene, prmA gene, fis gene, aroE gene, smg gene, smf gene, def gene, fmt gene, sun gene, trkA gene, mscL gene, zntR gene, rplQ gene, rpoA gene, rpsD gene, rpsK gene, rpsM gene, rpmJ gene, secY gene, rplO gene, rpmD gene, rpsE gene, rplR gene, rplF gene, rpsH gene, rpsN gene, rplE gene, rplX gene, rplN gene, rpsQ gene, rpmC gene, rplP gene, rpsC gene, rplV gene, rpsS gene, rplB gene, rplW gene, rplD gene, rplC gene, rpsJ gene, tufA gene, fusA gene, rpsG gene, rpsL gene, fkpA gene, slyX gene, slyD gene, kefB gene, prkB gene, crp gene, argD gene, pabA gene, fic gene, ppiA gene, nirB gene, nirD gene, nirC gene, cysG gene, php gene, trpS gene, gph gene, rpe gene, dam gene, damX gene, aroB gene, aroK gene, hofQ gene, mrcA gene, pckA gene, envZ gene, ompR gene, greB gene, feoA gene, feoB gene, bioH gene, gntT gene, malQ gene, malP gene, malT gene, rtcA gene, rtcB gene, rtcR gene, gipR gene, glpG gene, glpE gene, glpD gene, glgP gene, glgA gene, glgC gene, glgX gene, glgB gene, asd gene, gntU gene, gntU gene, gntK gene, gntR gene, ggt gene, ugpQ gene, ugpC gene, ugpE gene, ugpA gene, ugpB gene, livF gene, livG gene, livM gene, livH gene, livK gene, livJ gene, rpoH gene, ftsX gene, ftsE gene, ftsY gene, atzN gene, nikA gene, nikB gene, nikC gene, nikD gene, nikE gene, pitA gene, uspB gene, uspA gene, prlC gene, gor gene, arsR gene, arsB gene, arsC gene, slp gene, hdeB gene, hdeA gene, hdeD gene, gadA gene, treF gene, kdgK gene, dctA gene, dppF gene, dppD gene, dppC gene, dppB gene, dppA gene, tag gene, bisC gene, cspA gene, glyS gene, glyQ gene, xylB gene, xylA gene, xylF gene, xylG gene, xylH gene, xylR gene, bax gene, malS gene, avtA gene, lyx gene, sgbH gene, sgbU gene, sgbE gene, aldB gene, selB gene, selA gene, mtlA gene, mtlD gene, mtlR gene, lldP gene, lldR gene, lIdD gene, cysE gene, gpsA gene, secB gene, grxc gene, tdh gene, kbl gene, htrL gene, rfaD gene, rfaF gene, rfaC gene, rfaL gene, rfaK gene, rfaZ gene, rfaY gene, rfaJ gene, rfaI gene, rfaB gene, rfaS gene, rfaP gene, rfaG gene, rfaQ gene, kdtA gene, kdtB gene, mutM gene, rpmG gene, rpmB gene, radC gene, dfp gene, dut gene, ttk gene, pyrE gene, rph gene, dinD gene, gmk gene, rpoZ gene, spoT gene, spoU gene, recG gene, gitS gene, nlpA gene, uhpT gene, uhpC gene, uhpB gene, uhpA gene, ilvN gene, ilvB gene, ivbL gene, emrD gene, ibpB gene, ibpA gene, gyrB gene, recF gene, dnaN gene, dnaA gene, rpmH gene, rnpA gene, yidC gene, thdF gene, tnaL gene, tnaA gene, tnaB gene, bglB gene, bglF gene, bglG gene, phoU gene, pstB gene, pstA gene, pstC gene, pstS gene, glmS gene, glmU gene, atpC gene, uncD gene, atpG gene, atpA gene, atpH gene, atpF gene, atpE gene, atpB gene, atpI gene, gidB gene, gidA gene, mioC gene, asnC gene, pssR gene, ilvL gene, ilvG gene, ilvM gene, ilvE gene, ilvD gene, ilvA gene, ilvY gene, ilvC gene, ppiC gene, rep gene, gppA gene, rhlB gene, trxA gene, rhoL gene, rho gene, rfe gene, wzzE gene, nfrC gene, rffD gene, rffG gene, rffH gene, rffA gene, wzxE gene, rffT gene, wecG gene, aslB gene, aslA gene, hemY gene, hemX gene, hemD gene, hemC gene, cyaA gene, cyaY gene, dapF gene, xerC gene, uvrD gene, corA gene, radD gene, pldA gene, recQ gene, pldB gene, metR gene, metE gene, udp gene, ubiE gene, aarF gene, tatC gene, tatD gene, rfaH gene, ubiD gene, ubiB gene, fadA gene, fadB gene, pepQ gene, trkH gene, trkH gene, hemG gene, mobB gene, mobA gene, dsbA gene, polA gene, yihA gene, hemN gene, ntrC gene, ntrB gene, glnA gene, fdhE gene, fdoI gene, fdoH gene, fdoG gene, fdhD gene, sodA gene, kdgT gene, cpxA gene, cpxR gene, cpxP gene, pfkA gene, sbp gene, cdh gene, tpiA gene, fpr gene, glpX gene, glpK gene, glpF gene, menG gene, menA gene, hslU gene, hslV gene, ftsN gene, cytR gene, priA gene, rpmE gene, metJ gene, metB gene, metL gene, metF gene, katG gene, gldA gene, talC gene, ppc gene, argE gene, argC gene, argB gene, argH gene, oxyR gene, udhA gene, trmA gene, btuB gene, murI gene, murB gene, birA gene, coaA gene, tufB gene, secE gene, nusG gene, rplK gene, rplA gene, rplJ gene, rplL gene, rpoB gene, rpoC gene, htrC gene, thiH gene, thiG gene, thiS gene, thiF gene, thiE gene, thiC gene, rsd gene, hemE gene, nfi gene, hupA gene, hydH gene, hydG gene, purD gene, purH gene, metA gene, aceB gene, aceA gene, aceK gene, arp gene, iclR gene, metH gene, pepE gene, lysC gene, pgi gene, ubiC gene, ubiA gene, plsB gene, dgkA gene, lexA gene, dinF gene, qor gene, dnaB gene, alr gene, tyrB gene, napA gene, hobH gene, uvrA gene, ssb gene, soxS gene, soxR gene, acs gene, nrfA gene, nrfB gene, nrfC gene, nrfD gene, nrfE gene, nrfF gene, nrfG gene, gltP gene, fdhF gene, aisR gene, rpiB gene, proP gene, basS gene, basR gene, adiY gene, adi gene, melR gene, melA gene, melB gene, fumB gene, dcuB gene, dcuR gene, dcuS gene, lysU gene, cadA gene, cadB gene, cadC gene, cutA3 gene, cutA2 gene, cutA gene, dcuA gene, aspA gene, fxsA gene, groES gene, groEL gene, efp gene, ecnB gene, sugE gene, blc gene, ampC gene, frdD gene, frdC gene, frdB gene, frdA gene, genX gene, psd gene, yjeQ gene, yjeE gene, amiB gene, mutL gene, miaA gene, hfq gene, hflX gene, hflK gene, hflC gene, purA gene, vacB gene, sgaE gene, rpsF gene, priB gene, rpsR gene, rplI gene, fklB gene, cycA gene, cpdB gene, cysQ gene, msrA gene, chpS gene, chpB gene, ppa gene, fbp gene, mpl gene, pmbA gene, cybC gene, nrdG gene, nrdD gene, treC gene, treB gene, treR gene, mgtA gene, pyrI gene, pyrB gene, pyrBI gene, argl gene, valS gene, holC gene, xerB gene, idnR gene, idnT gene, idnO gene, idnD gene, idnK gene, fecE gene, fecD gene, fecC gene, fecB gene, fecA gene, fecR gene, fedI gene, dnaC gene, dnaT gene, bglJ gene, fhuF gene, rsmC gene, holD gene, rimI gene, prfC gene, osmY gene, deoC gene, deoA gene, deoB gene, deoD gene, lplA gene, smp gene, serB gene, radA gene, nadR gene, slt gene, trpR gene, gpmG gene, rob gene, creA gene, creB gene, creC gene, creD gene, arcA gene, lasT gene, thrL gene, and

acrE gene, argT gene, artI gene, artJ gene, artM gene, artP gene, artQ gene, bfr gene, chiA gene, chpA gene, dacC gene, deoR gene, dinG gene, elaA gene, elaB gene, elaC gene, elaD gene, envD gene, envR gene, fhuA gene, fhuB gene, fhuC gene, fhuD gene, folX gene, glnH gene, glnP gene, glnQ gene, gltJ gene, gltK gene, gltL gene, gspA gene, gspC gene, gspD gene, gspE gene, gspI gene, gspJ gene, gspK gene, gspL gene, hisJ gene, hisM gene, hisP gene, hisQ gene, hofD gene, hofF gene, hofG gene, hofH gene, ltaA gene, mdaA gene, mdaB gene, pinO gene, potF gene, potG gene, potH gene, potI gene, pshM gene, ptwB gene, ptwC gene, rhsB gene, rimK gene, xylU gene.

A specific example thereof is an E. coli mutant strain comprising chromosomal DNA that does not contain any gene selected from among genes excluding the above genes [1] and [2] among the genes shown in Table 1 or homologous genes thereof. {When the above genes [1] and [2] or homologous genes thereof comprise genes that wild-type E. coli strains do not originally contain (hereafter referred to as “NC genes”), however, the aforementioned E. coli mutant strain derived from the wild-type E. coli strain does not necessarily have the NC gene.} A more preferable example of the E. coli mutant strain of the present invention is an E. coli mutant strain comprising chromosomal DNA that does not contain DNA excluding the nucleotide sequences of the above genes [1] and [2] among the nucleotide sequences as shown in Table 1 and SEQ ID NO: 1 or DNA equivalent thereto. More preferred examples include the followings.

(2) The E. coli mutant strain according to (1) comprising chromosomal DNA that comprises DNA having the nucleotide sequences as shown in [1] to [69] below of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.

• [1] a nucleotide sequence of nucleotides 1 to 167064
• [2] a nucleotide sequence of nucleotides 172980 to 243964
• [3] a nucleotide sequence of nucleotides 253411 to 261963
• [4] a nucleotide sequence of nucleotides 387532 to 389138
• [5] a nucleotide sequence of nucleotides 403704 to 518029
• [6] a nucleotide sequence of nucleotides 532712 to 535504
• [7] a nucleotide sequence of nucleotides 550216 to 563940
• [8] a nucleotide sequence of nucleotides 608119 to 675103
• [9] a nucleotide sequence of nucleotides 689375 to 728820
• [10] a nucleotide sequence of nucleotides 739594 to 832551
• [11] a nucleotide sequence of nucleotides 848090 to 867637
• [12] a nucleotide sequence of nucleotides 883474 to 890997
• [13] a nucleotide sequence of nucleotides 909380 to 1048887
• [14] a nucleotide sequence of nucleotides 1097020 to 1198105
• [15] a nucleotide sequence of nucleotides 1225147 to 1630911
• [16] a nucleotide sequence of nucleotides 1641039 to 1642631
• [17] a nucleotide sequence of nucleotides 1650223 to 1964523
• [18] a nucleotide sequence of nucleotides 1993076 to 1996661
• [19] a nucleotide sequence of nucleotides 2028210 to 2033448
• [20] a nucleotide sequence of nucleotides 2044377 to 2069148
• [21] a nucleotide sequence of nucleotides 2081927 to 2104392
• [22] a nucleotide sequence of nucleotides 2116065 to 2146263
• [23] a nucleotide sequence of nucleotides 2187856 to 2200668
• [24] a nucleotide sequence of nucleotides 2229851 to 2255894
• [25] a nucleotide sequence of nucleotides 2263492 to 2270439
• [26] a nucleotide sequence of nucleotides 2286589 to 2386261
• [27] a nucleotide sequence of nucleotides 2395472 to 2423364
• [28] a nucleotide sequence of nucleotides 2434099 to 2472851
• [29] a nucleotide sequence of nucleotides 2482483 to 2505074
• [30] a nucleotide sequence of nucleotides 2514558 to 2755676
• [31] a nucleotide sequence of nucleotides 2789481 to 2795173
• [32] a nucleotide sequence of nucleotides 2800891 to 2804307
• [33] a nucleotide sequence of nucleotides 2810148 to 2825313
• [34] a nucleotide sequence of nucleotides 2830263 to 2910273
• [35] a nucleotide sequence of nucleotides 2910574 to 2985361
• [36] a nucleotide sequence of nucleotides 3033129 to 3077084
• [37] a nucleotide sequence of nucleotides 3078917 to 3110109
• [38] a nucleotide sequence of nucleotides 3134342 to 3165627
• [39] a nucleotide sequence of nucleotides 3172941 to 3360736
• [40] a nucleotide sequence of nucleotides 3370548 to 3411159
• [41] a nucleotide sequence of nucleotides 3420879 to 3453020
• [42] a nucleotide sequence of nucleotides 3469367 to 3618575
• [43] a nucleotide sequence of nucleotides 3636917 to 3854661
• [44] a nucleotide sequence of nucleotides 3865832 to 3867101
• [45] a nucleotide sequence of nucleotides 3877011 to 3926658
• [46] a nucleotide sequence of nucleotides 3938879 to 4059897
• [47] a nucleotide sequence of nucleotides 4079660 to 4086450
• [48] a nucleotide sequence of nucleotides 4099983 to 4139231
• [49] a nucleotide sequence of nucleotides 4149710 to 4235358
• [50] a nucleotide sequence of nucleotides 4251736 to 4298976
• [51] a nucleotide sequence of nucleotides 4311512 to 4313317
• [52] a nucleotide sequence of nucleotides 4329722 to 4409532
• [53] a nucleotide sequence of nucleotides 4423152 to 4496201
• [54] a nucleotide sequence of nucleotides 4509535 to 4517875
• [55] a nucleotide sequence of nucleotides 4599638 to 4641433
• [56] a nucleotide sequence of nucleotides 167065 to 172979
• [57] a nucleotide sequence of nucleotides 675104 to 689374
• [58] a nucleotide sequence of nucleotides 832552 to 848089
• [59] a nucleotide sequence of nucleotides 867638 to 883473
• [60] a nucleotide sequence of nucleotides 890998 to 909379
• [61] a nucleotide sequence of nucleotides 2386262 to 2395471
• [62] a nucleotide sequence of nucleotides 2423365 to 2434098
• [63] a nucleotide sequence of nucleotides 2472852 to 2482482
• [64] a nucleotide sequence of nucleotides 2505075 to 2514557
• [65] a nucleotide sequence of nucleotides 2910274 to 2910573
• [66] a nucleotide sequence of nucleotides 3165628 to 3172940
• [67] a nucleotide sequence of nucleotides 3411160 to 3420878
• [68] a nucleotide sequence of nucleotides 3453021 to 3469366
• [69] a nucleotide sequence of nucleotides 3618576 to 3636916
• (3) The E. coli mutant strain according to (1) or (2) comprising chromosomal DNA that does not comprise yafJ gene, yafK gene, yafQ gene, dinJ gene, yafL gene, yafM gene, fhiA gene, lafU gene, dinP gene, yafN gene, yafO gene, yafP gene, JW0225 gene, prfH gene, ykfI gene, yafW gene, yeeT gene, ykfG gene, yafX gene, ykfF gene, ykfB gene, yafY gene, ypjK gene, yafZ gene, ykfA gene, perR gene, JW0245 gene, JW0246 gene, JW0247 gene, ykfC gene, JW0249 gene, is5B gene, yi51 gene, mmuP gene, mmuM gene, afuC gene, JW0255 gene, insB gene, insA gene, JW0258 gene, yagB gene, yagA gene, yagE gene, yagF gene, yagG gene, yagH gene, yagI gene, argF gene, yfjI gene, yagJ gene, yagK gene, yagL gene, yagM gene, yagN gene, intF gene, yagP gene, yagQ gene, yagR gene, yagS gene, yagT gene, yagU gene, ykgJ gene, yagV gene, yagW gene, yagX gene, yagY gene, yagZ gene, ykgK gene, ykgL gene, ykgM gene, eaeH gene, JW0292 gene, JW0293 gene, ykgA gene, ykgB gene, ykgI gene, merA gene, ykgD gene, ykgE gene, ykgF gene, ykgG gene, ykgH gene, betA gene, betB gene, betI gene, betT gene, yahA gene, yahB gene, yahC gene, yahD gene, yahE gene, yahF gene, yahG gene, yahH gene, arcM gene, yahJ gene, yahK gene, yahL gene, yahM gene, yahN gene, yahO gene, prpR gene, bcpA gene, prpC gene, prpD gene, prpE gene, codB gene, codA gene, cynR gene, cynT gene, cynS gene, cynX gene, lacA gene, lacY gene, lacZ gene, lacd gene, mhpR gene, mhpA gene, mhpB gene, mhpC gene, mhpD gene, mhpF gene, mhpE gene, mhpT gene, yaiL gene, yaiM gene, adhC gene, yaiN gene, yaiO gene, JW0350 gene, yi2A gene, yi22 gene, yi24 gene, yi23 gene, yaiP gene, yaiS gene, tauA gene, tauB gene, tauC gene, ssiD gene, yaiT gene, JW0363 gene, JW0364 gene, yaiU gene, yaiV gene, ampH gene, sbmA gene, yaiW gene, yaiY gene, yaiZ gene, ddlA gene, yaiB gene, phoA gene, psiF gene, yaiC gene, ybbO gene, tesA gene, ybbA gene, ybbP gene, rhsD gene, ybbC gene, rhsE gene, ybbD gene, JW0490 gene, ybbB gene, ybbS gene, ybbT gene, ybbU gene, ybbQ gene, ybbV gene, allP gene, allA gene, ybbY gene, ybbZ gene, ylbA gene, alIC gene, ylbC gene, fdrA gene, ylbE gene, ylbF gene, arcC gene, intD gene, EXO_LAMBD gene, VRPP_LAMBD gene, JW0528 gene, JW0529 gene, VREN_LAMBD gene, emrE gene, ybcK gene, ybcL gene, ybcM gene, ybcN gene, ninE gene, ybcO gene, rus gene, ybcQ gene, JW0539.5 gene, nmpC gene, vlyS gene, ybcS gene, ybcT gene, vboR gene, ybcV gene, ybcW gene, nohB gene, ybcX gene, ybcY gene, JW0552 gene, appY gene, ompT gene, envY gene, ybcH gene, nfrA gene, nfrB gene, cusS gene, cusR gene, cusC gene, ylcC gene, cusB gene, cusA gene, pheP gene, ybdG gene, nfnB gene, ybdF gene, ybdJ gene, ybdK gene, hokE gene, yi81 gene, yi82 gene, ymcC gene, ymcD gene, cspH gene, cspG gene, sfa gene, yccL gene, yccM gene, torS gene, torT gene, torR gene, torC gene, torA gene, torD gene, yccD gene, cbpA gene, yccE gene, agp gene, yccJ gene, wrbA gene, yciG gene, ycdG gene, JW0992 gene, JW0993 gene, JW0994 gene, ycdK gene, JW0996 gene, JW0997 gene, ycdC gene, putA gene, yzpU gene, putP gene, JW1002 gene, ycdO gene, ycdB gene, phoH gene, ycdP gene, ycdQ gene, ycdR gene, ycdS gene, ycdT gene, JW1012 gene, JW1013 gene, JW1014 gene, ycdU gene, ymfD gene, ymfE gene, lit gene, intE gene, VXIS_BPP21 gene, ymfH gene, ymfI gene, ymfJ gene, RPC2_BPP22 gene, JW1132 gene, ymfL gene, ymfM gene, ymfN gene, ymfR gene, ymfO gene, JW1138 gene, JW1139 gene, ycfK gene, ymfS gene, ycfA gene, ycfE gene, pin gene, mcrA gene, JW1146 gene, ycgW gene, ycgX gene, ycgE gene, JW1150 gene, ycgZ gene, ymgA gene, JW1153 gene, ymgC gene, yahA gene, JW1156 gene, JW1157 gene, aidA gene, ymgD gene, JW1160 gene, JW1161 gene, JW1162 gene, ydfH gene, ydfZ gene, ydfI gene, shiA gene, JW1537 gene, tnpR gene, ydfM gene, Y314_LAMBD gene, nohA gene, ydfO gene, yccL gene, cspI gene, ydfR gene, vlyS gene, cspB gene, cspF gene, regQ_LAMBD gene, JW1552 gene, rem gene, hokD gene, relE gene, relB gene, ydfV gene, fixA gene, ydfW gene, ydfX gene, dicC gene, sdaC gene, yqeH gene, yqeI gene, yqeJ gene, yqeK gene, ygeF gene, ygeG gene, ygeH gene, ygeI gene, JW2822 gene, ygeK gene, JW2824 gene, JW2825 gene, JW2830 gene, ygeP gene, JW2832 gene, nlpD gene, yagR gene, ygeT gene, JW2836 gene, ygeV gene, ygew gene, dpaL gene, ygeY gene, ygeZ gene, arcL gene, yqeB gene, JW2844 gene, ygfJ gene, gltD gene, JW2847 gene, ygfM gene, yagR gene, ygfO gene, ygfP gene, ygfQ gene, ygfR gene, ygfS gene, ygfT gene, pbuX gene, idi gene, epsM gene, exeL gene, exeC gene, JW2938 gene, JW2939 gene, JW2940 gene, JW2941 gene, yghK gene, glcB gene, glcG gene, glcF gene, glcD gene, glcC gene, JW2948 gene, yghQ gene, yghR gene, yghS gene, yghT gene, yihL gene, yihM gene, yihN gene, yshA gene, yihO gene, yihP gene, yihQ gene, yihR gene, yihS gene, yihT gene, yihU gene, yihV gene, yihW gene, yihX gene, rbn gene, yihZ gene, yiiD gene, yiiE gene, yiiF gene, yiiG gene, frvR gene, frvX gene, frvB gene, frvA gene, yiiL gene, rhaD gene, rhaA gene, rhaB gene, rhaS gene, rhaR gene, rhaT gene, ptsA gene, JW3920 gene, frwC gene, frwB gene, pflD gene, pflC gene, frwD gene, yijO gene, yijP gene, JW3986 gene, yjbF gene, yjbG gene, yjbH gene, JW3989.5 gene, yjbA gene, xylE gene, maiG gene, malF gene, malE gene, malK gene, lamB gene, malM gene, yjbI gene, yjcP gene, yjcQ gene, yjcR gene, yjcS gene, alsK gene, alsE gene, alsC gene, alsA gene, alsB gene, phnQ gene, phnP gene, phnO gene, phnN gene, phnM gene, phnL gene, phnK gene, phnJ gene, phnI gene, phnH gene, phnG gene, phnF gene, phnE gene, phnD gene, phnC gene, phnB gene, phnA gene, yjdA gene and yjcZ gene, or homologous gene thereof.
• (4) The E. coli mutant strain according to any one of (1) to (3) comprising chromosomal DNA that does not comprise DNA having the nucleotide sequences as shown in [1] to [18] below of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.
• [1] a nucleotide sequence of nucleotides 243965 to 253410
• [2] a nucleotide sequence of nucleotides 261964 to 387531
• [3] a nucleotide sequence of nucleotides 389139 to 403703
• [4] a nucleotide sequence of nucleotides 518030 to 532711
• [5] a nucleotide sequence of nucleotides 535505 to 550215
• [6] a nucleotide sequence of nucleotides 563941 to 608118
• [7] a nucleotide sequence of nucleotides 1048888 to 1097019
• [8] a nucleotide sequence of nucleotides 1198106 to 1225146
• [9] a nucleotide sequence of nucleotides 1630912 to 1641038
• [10] a nucleotide sequence of nucleotides 1642632 to 1650222
• [11] a nucleotide sequence of nucleotides 2985362 to 3033128
• [12] a nucleotide sequence of nucleotides 3110110 to 3134341
• [13] a nucleotide sequence of nucleotides 4059898 to 4079659
• [14] a nucleotide sequence of nucleotides 4086451 to 4099982
• [15] a nucleotide sequence of nucleotides 4139232 to 4149709
• [16] a nucleotide sequence of nucleotides 4235359 to 4251735
• [17] a nucleotide sequence of nucleotides 4298977 to 4311511
• [18] a nucleotide sequence of nucleotides 4313318 to 4329721
• (5) The E. coli mutant strain according to any one of (1) to (4) comprising chromosomal DNA that does not comprise yidF gene, yidG gene, yidH gene, yidI gene, yidJ gene, yidK gene, yidL gene, JW3657 gene, glvG gene, glvB gene, glvC gene, yidP gene, yidE gene, yidQ gene, yidR gene, yidS gene, dgoT gene, dgoA gene, dgoK gene, dgoR gene, yidW gene, yidX gene, yidA gene, yidB gene, asnA gene, yieM gene, JW3724 gene, yieN gene, kup gene, rbsD gene, rbsA gene, rbsC gene, rbsB gene, rbsK gene, rbsR gene and yieO gene, or homologous gene thereof.
• (6) The E. coli mutant strain according to any one of (1) to (5) comprising chromosomal DNA that does not comprise DNA having the nucleotide sequences as shown in [1] to [3] below of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.
• [1] a nucleotide sequence of nucleotides3854662 to 3865831
• [2] a nucleotide sequence of nucleotides3 867102 to 3877010
• [3] a nucleotide sequence of nucleotides 3926659 to 3938878
• (7) The E. coli mutant strain according to any one of (1) to (6) comprising chromosomal DNA that does not comprise yecT gene, flhE gene, flhA gene, flhB gene, cheZ gene, cheY gene, cheB gene, cheR gene, tap gene, tar gene, cheW gene, cheA gene, motB gene, motA gene, fihC gene, flhD gene, JW1882 gene, JW1883 gene, yecG gene, otsA gene, otsB gene, araH gene, araG gene, araF gene, yecI gene, yecJ gene, JW1892 gene, ftn gene, yecH gene, tyrP gene, uvrC gene, uvrY gene, yecF gene, sdiA gene, yecC gene, yecS gene, yedO gene, fliY gene, fliZ gene, fliA gene, fliC gene, fliD gene, fliS gene, fliT gene, amyA gene, yedD gene, yedE gene, yedF gene, yedK gene, yedL gene, yedN gene, yedM gene, fliE gene, fliF gene, fliG gene, fliH gene, fliI gene, fliJ gene, fliK gene, fliL gene, fliM gene, fliN gene, fliO gene, fliP gene, fliQ gene, fliR gene, rcsA gene, dsrB gene, JW1937 gene, yedA gene, vsr gene, dcm gene, yedJ gene, JW1946 gene, ompC gene, JW1948 gene, yedS gene, yedU gene, yedV gene, copR gene, yedX gene, JW1954 gene, JW1955 gene, JW1956 gene, JW1974 gene, JW1979 gene, JW1980 gene, yeeP gene, flu gene, JW1983 gene, yeeS gene, yeeU gene, yeeV gene, yeeW gene, yefJ gene, JW2013 gene, yefJ gene, yefI gene, wbbJ gene, yefG gene, rfc gene, glf gene, rfbX gene, rfbC gene, rfbA gene, rfbD gene and rfbB gene, or homologous gene thereof.
• (8) The E. coli mutant strain according to any one of (1) to (7) comprising chromosomal DNA that does not comprise DNA having the nucleotide sequences as shown in [1] to [5] below of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.
• [1] a nucleotide sequence of nucleotides 964524 to 1993075
• [2] a nucleotide sequence of nucleotides 1996662 to 2028209
• [3] a nucleotide sequence of nucleotides 2033449 to 2044376
• [4] a nucleotide sequence of nucleotides 2069149t to 2081926
• [5] a nucleotide sequence of nucleotides 2104393 to 2116064
• (9) The E. coli mutant strain according to any one of (1) to (8) comprising chromosomal DNA that does not comprise intA gene, yfjH gene, alpA gene, yfjI gene, JW2606 gene, yfjJ gene, yfjK gene, yfjL gene, yfjM gene, yfjN gene, yfjO gene, yfjP gene, yfjQ gene, yfjR gene, ypjK gene, yfjS gene, yfjT gene, yfjU gene, yfjV gene, JW2622 gene, yfjW gene, JW2623.5 gene, yfjX gene, yfjY gene, yfjZ gene, ypjF gene, ypjA gene, JW2629 gene, JW2630 gene, JW2631 gene, JW2632 gene, JW2633 gene, JW2634 gene, ygaE gene, yzzM gene, yqaE gene, JW2642 gene, ygaP gene, stpA gene, JW2645 gene, ygaC gene, ygaM gene, nrdH gene, nrdI gene, nrdE gene, proV gene, proW gene, proX gene, JW2655 gene, JW2656 gene, ygaZ gene, ygaH gene, srlA gene, srlB gene, srlD gene, gutM gene, srlR gene and gutQ gene, or homologous gene thereof.
• (10) The E. coli mutant strain according to any one of (1) to (9) comprising chromosomal DNA that does not comprise DNA having the nucleotide sequences as shown in [1] to [4] below of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.
• [1] a nucleotide sequence of nucleotides 2755677 to 2789480
• [2] a nucleotide sequence of nucleotides 2795174 to 2800890
• [3] a nucleotide sequence of nucleotides 2804308 to 2810147
• [4] a nucleotide sequence of nucleotides 2825314 to 2830262
• (11) The E. coli mutant strain according to any one of (1) to (10) comprising chromosomal DNA that does not comprise gltF gene, yhcA gene, yhcD gene, yhcE gene, yhcF gene, yhcG gene, yhcH gene, nanK gene and nanE gene, or homologous gene thereof.
• (12) The E. coli mutant strain according to any one of (1) to (11) comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 3360737 to 3370547 of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.
• (13) The E. coli mutant strain according to any one of (1) to (12) comprising chromosomal DNA that does not comprise yegE gene, alka gene, yegD gene, JW2055 gene, JW2056 gene, JW2057 gene, JW2058 gene, yegM gene, yegN gene, yegO gene, yegB gene, baeS gene, baeR gene, JW2065 gene, yegQ gene, ogrK gene, JW2068 gene, JW2069 gene, yegS gene, gatR gene, JW2072 gene, JW2073 gene, gatR gene, gatD gene, gatC gene, gatB gene, gatA gene, gatZ gene, gatY gene, fbaB gene, xegT gene, yegU gene, yegV gene, yegW gene, yegX gene, molR gene, yehI gene, yehK gene, yehL gene, yehO gene, yehP gene, yehQ gene, yehR gene, yehS gene, yehT gene, yehU gene, yehV gene, yehW gene, yehX gene, yehY gene, yehZ gene, bglX gene, dld gene, pbpG gene, yohC gene, yeiQ gene, yeiR gene, yeiU gene, spr gene, rtn gene, yejA gene, yejB gene, yejE gene, yejF gene, yejG gene, bcr gene, rsuA gene, yejH gene, yeiI gene, yeiJ gene, yeiK gene, yeiL gene, yeiM gene, yeiN gene and yeiC gene, or homologous gene thereof.
• (14) The E. coli mutant strain according to any one of (1) to (13) comprising chromosomal DNA that does not comprise DNA having the nucleotide sequences as shown in [1] to [4] below of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.
• [1] a nucleotide sequence of nucleotides 2146264 to 2187855
• [2] a nucleotide sequence of nucleotides 2200669 to 2229850
• [3] a nucleotide sequence of nucleotides 2270440 to 2286588
• [4] a nucleotide sequence of nucleotides 2255895 to 2263491
• (15) The E. coli mutant strain according to any one of (1) to (14) comprising chromosomal DNA that does not comprise cmtA gene, cmtB gene, or homologous gene thereof.
• (16) The E. coli mutant strain according to any one of (1) to (15) comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 3077085 to 3078916 of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.
• (17) The E. coli mutant strain according to any one of (1) to (16) comprising chromosomal DNA that does not comprise intB gene, JW4228 gene, yjgW gene, yjgX gene, yjgY gene, yjgZ gene, yi41 gene, yi42 gene, yjhB gene, yjhC gene, yjhD gene, yjhE gene, yi91 gene, JW4244 gene, JW4245 gene, JW4246 gene, yjhU gene, yjhF gene, yjhG gene, yjhH gene, yjhI gene, sgcR gene, sgcE gene, sgcA gene, sgcQ gene, sgcC gene, JW4266.5 gene, sgcX gene, yjhP gene, yjhQ gene, JW4269.5 gene, JW4270 gene, JW4270.5 gene, yjhR gene, yjhS gene, yjhT gene, yjhA gene, fimB gene, fimE gene, fimA gene, fimI gene, fimC gene, fimD gene, fimF gene, fimG gene, fimH gene, gntP gene, uxuA gene, uxuB gene, uxuR gene, yjiC gene, yjiD gene, yjiE gene, iadA gene, yjiG gene, yjiH gene, yjiI gene, yjiJ gene, yjiK gene, JW4296.5 gene, yjiL gene, yjiM gene, yjiN gene, yjiO gene, yjiP gene, yjiQ gene, yjiR gene, yjiS gene, yjiT gene, yjiV gene, mcrD gene, mcrC gene, mcrB gene, yjiW gene, hsdS gene, hsdM gene, hsdR gene, mrr gene, yjiA gene, yjiX gene, yjiY gene, tsr gene, yjjL gene, JW4320 gene, yjjM gene, yjjN gene, mdoB gene and yjjA gene, or homologous gene thereof.
• (18) The E. coli mutant strain according to any one of (1) to (17) comprising chromosomal DNA that does not comprise DNA having the nucleotide sequences as shown in [1] and/or [2] below of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.
• [1] a nucleotide sequence of nucleotides 4496202 to 4509534
• [2] a nucleotide sequence of nucleotides 4517876 to 4599637
• (19) The E. coli mutant strain according to any one of (1) to (18) comprising chromosomal DNA that does not comprise kdpF gene, ybfA gene, rhsC gene, JW0690 gene, ybfB gene, rhsA gene, ybfC gene, ybfD gene, ybfL gene, ybfD gene and ybgA gene, or homologous gene thereof.
• (20) The E. coli mutant strain according to any one of (1) to (19) comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 728821 to 739593 of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.
• (21) The E. coli mutant strain according to any one of (1) to (20) comprising chromosomal DNA that does not comprise yjfl gene, yjfJ gene, yjfK gene, yjfL gene, yjfM gene, yjfC gene, aidB gene, yjfN gene, yjfO gene, yjfP gene, yjfQ gene, yjfR gene, sgaT gene, sgaB gene, sgaA gene, sgaH gene and sgaU gene, or homologous gene thereof.
• (22) The E. coli mutant strain according to any one of (1) to (21) comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 4409533 to 4423151 of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.

Further, the mutant of the present invention includes the followings.

• (23) An E. coli mutant strain comprising the gene (1) [1] above, spr gene, bcr gene, rsuA gene, or homologous gene thereof and comprising chromosomal DNA that is at least 933 kbp shorter than that of a wild-type E. coli strain, which may be the mutant strain comprising chromosomal DNA that does not comprise any gene excluding the above genes that are present on chromosomal DNA of a wild-type E. coli strain or homologous genes thereof, preferably the mutant strain comprising chromosomal DNA that does not contain gene other than genes contained in Δ43 region of Table 1; more preferably the mutant strain comprising chromosomal DNA that does not contain DNA other than DNA comprising nucleotide sequence corresponding to Δ43 region of Table 1 or DNA equivalent thereto. Further more preferably, the mutant of the present invention includes the followings.
• (24) The E. coli mutant strain according to (23) comprising chromosomal DNA that comprises DNA having the nucleotide sequences as shown in [1] to [55] of (2) above and DNA having a nucleotide sequence comprising nucleotides 2270440 to 2286588 of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.
• (25) The E. coli mutant strain according to (23) or (24) comprising chromosomal DNA that does not comprise yafJ gene, yafK gene, yafQ gene, dinJ gene, yafL gene, yafM gene, fhiA gene, lafU gene, dinP gene, yafN gene, yafO gene, yafP gene, JW0225 gene, prfH gene, ykfI gene, yafW gene, yeeT gene, ykfG gene, yafX gene, ykfF gene, ykfB gene, yafY gene, ypjK gene, yafZ gene, ykfA gene, perR gene, JW0245 gene, JW0246 gene, JW0247 gene, ykfC gene, JW0249 gene, is51 gene, yi51 gene, mmuP gene, mmuM gene, afuC gene, JW0255 gene, insB gene, insA gene, JW0258 gene, yagB gene, yagA gene, yagE gene, yagF gene, yagG gene, yagH gene, yagI gene, argF gene, yfjI gene, yagJ gene, yagK gene, yagL gene, yagM gene, yagN gene, intF gene, yagP gene, yagQ gene, yagR gene, yagS gene, yagT gene, yagU gene, ykgJ gene, yagV gene, yagW gene, yagX gene, yagY gene, yagZ gene, ykgK gene, ykgL gene, ykgM gene, eaeH gene, JW0292 gene, JW0293 gene, ykgA gene, ykgB gene, ykgI gene, merA gene, ykgD gene, ykgE gene, ykgF gene, ykgG gene, ykgH gene, betA gene, betB gene, betI gene, betT gene, yahA gene, yahB gene, yahC gene, yahD gene, yahE gene, yahF gene, yahG gene, yahH gene, arcM gene, yahJ gene, yahK gene, yahL gene, yahM gene, yahN gene, yahO gene, prpR gene, bcpA gene, prpC gene, prpD gene, prpE gene, codB gene, codA gene, cynR gene, cynT gene, cynS gene, cynX gene, lacA gene, lacY gene, lacZ gene, lacI gene, mhpR gene, mhpA gene, mhpB gene, mhpC gene, mhpD gene, mhpF gene, mhpE gene, mhpT gene, yaiL gene, yaiM gene, adhC gene, yaiN gene, yaiO gene, JW0350 gene, yi2A gene, yi22 gene, yi24 gene, yi23 gene, yaiP gene, yaiS gene, tauA gene, tauB gene, tauC gene, ssiD gene, yaiT gene, JW0363 gene, JW0364 gene, yaiU gene, yaiV gene, ampH gene, sbmA gene, yaiW gene, yaiY gene, yaiZ gene, ddlA gene, yaiB gene, phoA gene, psiF gene, yaiC gene, ybbO gene, tesA gene, ybbA gene, ybbP gene, rhsD gene, ybbC gene, rhsE gene, ybbD gene, JW0490 gene, ybbB gene, ybbS gene, ybbT gene, ybbU gene, ybbQ gene, ybbV gene, allP gene, allA gene, ybbY gene, ybbZ gene, ylbA gene, allC gene, ylbC gene, fdrA gene, ylbE gene, ylbE gene, ylbE gene, ylbF gene, arcC gene, intD gene, EXO_LAMBD gene, VRPP_LAMBD gene, JW0528 gene, JW0529 gene, VREN_LAMBD gene, emrE gene, ybcK gene, ybcL gene, ybcM gene, ybcN gene, ninE gene, ybcO gene, rus gene, ybcQ gene, JW0539.5 gene, nmpC gene, vlyS gene, ybcS gene, ybcT gene, vboR gene, ybcV gene, ybcW gene, nohB gene, ybcX gene, ybcY gene, JW0552 gene, appY gene, ompT gene, envY gene, ybcH gene, nfrA gene, nfrB gene, cusS gene, cusR gene, cusC gene, ylcC gene, cusB gene, cusA gene, pheP gene, ybdG gene, nfnB gene, ybdF gene, ybdJ gene, ybdK gene, hokE gene, yi81 gene, yi82 gene, ymcC gene, ymcD gene, cspH gene, cspG gene, sfa gene, yccL gene, yccM gene, torS gene, torT gene, torR gene, torC gene, torA gene, torD gene, yccD gene, cbpA gene, yccE gene, agp gene, yccJ gene, wrbA gene, yciG gene, ycdG gene, JW0992 gene, JW0993 gene, JW0994 gene, ycdK gene, JW0996 gene, JW0997 gene, ycdC gene, putA gene, yzpU gene, putP gene, JW1002 gene, ycdO gene, ycdB gene, phoH gene, ycdP gene, ycdQ gene, ycdR gene, ycdR gene, ycdS gene, ycdT gene, JW1012 gene, JW1013 gene, JW1014 gene, ycdU gene, ymfD gene, ymfE gene, lit gene, intE gene, VXIS_BPP21 gene, ymfH gene, ymfI gene, ymfJ gene, RPC2_BPP22 gene, JW1132 gene, ymfL gene, ymfM gene, ymfN gene, ymfR gene, ymfO gene, JW1138 gene, JW1139 gene, ycfK gene, ymfS gene, ycfA gene, ycfE gene, pin gene, mcrA gene, JW1146 gene, ycgW gene, ycgX gene, ycgE gene, JW1150 gene, ycgZ gene, ymgA gene, JW1153 gene, ymgC gene, yahA gene, JW1156 gene, JW1157 gene, aidA gene, ymgD gene, JW1160 gene, JW1161 gene, JW1162 gene, ydfH gene, ydfZ gene, ydfI gene, shiA gene, JW1537 gene, tnpR gene, ydfM gene, Y314_LAMBD gene, nohA gene, ydfO gene, yccL gene, cspI gene, ydfR gene, vlyS gene, cspB gene, cspF gene, regQ_LAMBD gene, JW1552 gene, rem gene, hokD gene, relE gene, relB gene, ydfV gene, flxA gene, ydfW gene, ydfX gene, dicC gene, sdaC gene, yqeH gene, yqeI gene, yqeJ gene, yqeK gene, ygeF gene, ygeG gene, ygeH gene, ygeI gene, JW2822 gene, ygeK gene, JW2824 gene, JW2825 gene, JW2830 gene, ygeP gene, JW2832 gene, nlpD gene, yagR gene, ygeT gene, JW2836 gene, ygeV gene, ygeW gene, dpaL gene, ygeY gene, ygeZ gene, areL gene, yqeB gene, JW2844 gene, ygfJ gene, gltD gene, JW2847 gene, ygfM gene, yagR gene, ygfO gene, ygfP gene, ygfQ gene, ygfR gene, ygfS gene, ygfT gene, pbuX gene, idi gene, epsM gene, exeL gene, exeC gene, JW2938 gene, JW2939 gene, JW2940 gene, JW2941 gene, yghK gene, glcB gene, glcG gene, glcF gene, glcD gene, glcC gene, JW2948 gene, yghQ gene, yghR gene, yghS gene, yghT gene, yihL gene, yihM gene, yihN gene, yshA gene, yihO gene, yihP gene, yihQ gene, yihR gene, yihS gene, yihT gene, yihU gene, yihV gene, yihW gene, yihX gene, rbn gene, yihZ gene, yiiD gene, yiiE gene, yiiF gene, yiiG gene, frvR gene, frvX gene, frvB gene, frvA gene, yiiL gene, rhaD gene, rhaA gene, rhaB gene, rhaS gene, rhaR gene, rhaT gene, ptsA gene, JW3920 gene, frwC gene, frwB gene, pflD gene, pflC gene, frwD gene, yijO gene, yijP gene, JW3986 gene, yjbF gene, yjbG gene, yjbH gene, JW3989.5 gene, yjbA gene, xylE gene, malG gene, malF gene, malE gene, malK gene, lamB gene, malM gene, yjbI gene, yjcP gene, yjcQ gene, yjcR gene, yjcS gene, alsK gene, alsE gene, alsC gene, alsA gene, alsB gene, phnQ gene, phnP gene, phnO gene, phnN gene, phnM gene, phnL gene, phnK gene, phnJ gene, phnI gene, phnH gene, phnG gene, phnF gene, phnE gene, phnD gene, phnC gene, phnB gene, phnA gene, yjdA gene, yjcZ gene, yidF gene, yidG gene, yidH gene, yidI gene, yidJ gene, yidK gene, yidL gene, JW3657 gene, glvG gene, glvB gene, glvC gene, yidP gene, yidE gene, yidQ gene, yidR gene, yidS gene, dgoT gene, dgoA gene, dgoK gene, dgoR gene, yidW gene, yidX gene, yidA gene, yidB gene, asnA gene, yieM gene, JW3724 gene, yieN gene, kup gene, rbsD gene, rbsA gene, rbsC gene, rbsB gene, rbsK gene, rbsR gene, yieO gene, yecT gene, flhE gene, flhA gene, flhB gene, cheZ gene, cheY gene, cheB gene, cheR gene, tap gene, tar gene, cheW gene, cheA gene, motB gene, motA gene, flhC gene, flhD gene, JW1882 gene, JW1883 gene, yecG gene, otsA gene, otsB gene, araH gene, araG gene, araF gene, yecI gene, yecJ gene, JW1892 gene, ftn gene, yecH gene, tyrP gene, uvrC gene, uvrY gene, yecF gene, sdiA gene, yecC gene, yecS gene, yedO gene, fliY gene, fliZ gene, fliA gene, fliC gene, fliD gene, fliS gene, fliT gene, amyA gene, yedD gene, yedE gene, yedF gene, yedK gene, yedL gene, yedN gene, yedM gene, fliE gene, fliF gene, fliG gene, fliH gene, fliI gene, fliJ gene, fliK gene, fliL gene, fliM gene, fliN gene, fliO gene, flip gene, fliQ gene, fliR gene, rcsA gene, dsrB gene, JW1937 gene, yedA gene, vsr gene, dcm gene, yedJ gene, JW1946 gene, ompC gene, JW1948 gene, yedS gene, yedU gene, yedV gene, copR gene, yedX gene, JW1954 gene, JW1955 gene, JW1956 gene, JW1974 gene, JW1979 gene, JW1980 gene, yeeP gene, flu gene, JW1983 gene, yeeS gene, yeeT gene, yeeU gene, yeeV gene, yeeW gene, yefJ gene, JW2013 gene, yefJ gene, yefI gene, wbbJ gene, yefG gene, rfc gene, glf gene, rfbX gene, rfbC gene, rfbA gene, rfbD gene, rfbB gene, intA gene, yfjH gene, alpA gene, yfjI gene, JW2606 gene, yfjJ gene, yfjK gene, yfjL gene, yfjM gene, yfjN gene, yfjO gene, yfjP gene, yfjQ gene, yfjR gene, ypjK gene, yfjS gene, yfjT gene, yfjU gene, yfjV gene, JW2622 gene, yfjW gene, JW2623.5 gene, yfjX gene, yfjY gene, yfjZ gene, ypjF gene, ypjA gene, JW2629 gene, JW2630 gene, JW2631 gene, JW2632 gene, JW2633 gene, JW2634 gene, ygaE gene, yzzM gene, yqaE gene, JW2642 gene, ygaP gene, stpA gene, JW2645 gene, ygaC gene, ygaM gene, nrdH gene, nrdI gene, nrdE gene, proV gene, proW gene, proX gene, JW2655 gene, JW2656 gene, ygaZ gene, ygaH gene, srlA gene, srlB gene, srlD gene, gutM gene, srlR gene, gutQ gene, gltF gene, yhcA gene, yhcD gene, yhcE gene, yhcE gene, yhcF gene, yhcG gene, yhcH gene, nanK gene, nanE gene, yegE gene, alkA gene, yegD gene, JW2055 gene, JW2056 gene, JW2057 gene, JW2058 gene, yegM gene, yegN gene, yegO gene, yegB gene, baeS gene, baeR gene, JW2065 gene, yegQ gene, ogrK gene, JW2068 gene, JW2069 gene, yegS gene, gatR gene, JW2072 gene, JW2073 gene, gatR gene, gatD gene, gatC gene, gatB gene, gatA gene, gatZ gene, gatY gene, fbaB gene, xegT gene, yegU gene, yegV gene, yegW gene, yegX gene, molR gene, yehI gene, yehK gene, yehL gene, yehO gene, yehP gene, yehQ gene, yehR gene, yehS gene, yehT gene, yehU gene, yehV gene, yehW gene, yehX gene, yehY gene, yehZ gene, bglX gene, dld gene, pbpG gene, yohC gene, yeiI gene, yeiJ gene, yeiK gene, yeiL gene, yeiM gene, yeiN gene, yeiC gene, cmtA gene, cmtB gene, intB gene, JW4228 gene, yjgW gene, yjgX gene, yjgY gene, yjgZ gene, yi41 gene, yi42 gene, yjhB gene, yjhC gene, yjhD gene, yjhE gene, yi91 gene, JW4244 gene, JW4245 gene, JW4246 gene, yjhU gene, yjhF gene, yjhG gene, yjhH gene, yjhI gene, sgcR gene, sgcE gene, sgcA gene, sgcQ gene, sgcC gene, JW4266.5 gene, sgcX gene, yjhP gene, yjhQ gene, JW4269.5 gene, JW4270 gene, JW4270.5 gene, yjhR gene, yjhS gene, yjhT gene, yjhA gene, fimB gene, fimE gene, fimnA gene, fimI gene, fimC gene, fimD gene, fimF gene, fimG gene, fimH gene, gntP gene, uxuA gene, uxuB gene, uxuR gene, yjiC gene, yjiD gene, yjiE gene, iadA gene, yjiG gene, yjiH gene, yjiI gene, yjiJ gene, yjiK gene, JW4296.5 gene, yjiL gene, yjiM gene, yjiN gene, yjiO gene, yjiP gene, yjiQ gene, yjiR gene, yjiS gene, yjiT gene, yjiV gene, mcrD gene, mcrC gene, mcrB gene, yjiW gene, hsdS gene, hsdM gene, hsdR gene, mrr gene, yjiA gene, yjiX gene, yjiY gene, tsr gene, yjjL gene, JW4320 gene, yjjM gene, yjjN gene, mdoB gene, yjjA gene, kdpF gene, ybfA gene, rhsC gene, JW0690 gene, ybfB gene, rhsA gene, ybfC gene, ybfL gene, ybfD gene, ybgA gene, yjfI gene, yjfJ gene, yjfK gene, yjfL gene, yjfM gene, yjfC gene, aidB gene, yjfN gene, yjfO gene, yjfP gene, yjfQ gene, yjfR gene, sgaT gene, sgaB gene, sgaA gene, sgaH gene, sgaU gene, rhsB gene, JW3448 gene, yhhH gene, yibA gene, yhhI gene, yhhJ gene, yhiH gene, yhiI gene, yhiJ gene, yhiK gene, yhiL gene, yhiM gene, yhiN gene, JW0782 gene, ybiA gene, dinG gene, ybiB gene, ybiC gene, ybiJ gene, ybiI gene, ybiX gene, ybiL gene, ybiM gene, ybiN gene, ybiO gene, glnQ gene, glnP gene, glnH gene, yliA gene, yliB gene, yliC gene, yliD gene, JW0817 gene, JW0818 gene, yliG gene, yliH gene, yliI gene, yliJ gene, dacC gene, deoR gene, ybjG gene, ybjC gene, mdaA gene, rimK gene, ybjN gene, potF gene, potG gene, potH gene, potI gene, ybjO gene, ybjF gene, artJ gene, artM gene, artQ gene, artI gene, artP gene, ybjP gene, JW0850 gene, JW0851 gene, JW0852 gene, ybjT gene and ltaA gene, or homologous gene thereof.
• (26) The E. coli mutant strain according to any one of (23) to (25) comprising chromosomal DNA that does not comprise DNA having the nucleotide sequences as shown in [1] to [43] of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.
• [1] a nucleotide sequence of nucleotides 243965 to 253410
• [2] a nucleotide sequence of nucleotides 261964 to 387531
• [3] a nucleotide sequence of nucleotides 389139 to 403703
• [4] a nucleotide sequence of nucleotides 518030 to 532711
• [5] a nucleotide sequence of nucleotides 535505 to 550215
• [6] a nucleotide sequence of nucleotides 563941 to 608118
• [7] a nucleotide sequence of nucleotides 728821 to 739593
• [8] a nucleotide sequence of nucleotides 832552 to 848089
• [9] a nucleotide sequence of nucleotides 867638 to 883473
• [10] a nucleotide sequence of nucleotides 890998 to 909379
• [11] a nucleotide sequence of nucleotides 1048888 to 1097019
• [12] a nucleotide sequence of nucleotides 1198106 to 1225146
• [13] a nucleotide sequence of nucleotides 1630912 to 1641038
• [14] a nucleotide sequence of nucleotides 1642632 to 1650222
• [15] a nucleotide sequence of nucleotides 1964524 to 1993075
• [16] a nucleotide sequence of nucleotides 1996662 to 2028209
• [17] a nucleotide sequence of nucleotides 2033449 to 2044376
• [18] a nucleotide sequence of nucleotides 2069149 to 2081926
• [19] a nucleotide sequence of nucleotides 2104393 to 2116064
• [20] a nucleotide sequence of nucleotides 2146264 to 2187855
• [21] a nucleotide sequence of nucleotides 2200669 to 2229850
• [22] a nucleotide sequence of nucleotides 2255895 to 2263491
• [23] a nucleotide sequence of nucleotides 2755677 to 2789480
• [24] a nucleotide sequence of nucleotides 2795174 to 2800890
• [25] a nucleotide sequence of nucleotides 2804308 to 2810147
• [26] a nucleotide sequence of nucleotides 2825314 to 2830262
• [27] a nucleotide sequence of nucleotides 2985362 to 3033128
• [28] a nucleotide sequence of nucleotides 3077085 to 3078916
• [29] a nucleotide sequence of nucleotides 3110110 to 3134341
• [30] a nucleotide sequence of nucleotides 3360737 to 3370547
• [31] a nucleotide sequence of nucleotides 3618576 to 3636916
• [32] a nucleotide sequence of nucleotides 3854662 to 3865831
• [33] a nucleotide sequence of nucleotides 3867102 to 3877010
• [34] a nucleotide sequence of nucleotides 3926659 to 3938878
• [35] a nucleotide sequence of nucleotides 4059898 to 4079659
• [36] a nucleotide sequence of nucleotides 4086451 to 4099982
• [37] a nucleotide sequence of nucleotides 4139232 to 4149709
• [38] a nucleotide sequence of nucleotides 4235359 to 4251735
• [39] a nucleotide sequence of nucleotides 4298977 to 4311511
• [40] a nucleotide sequence of nucleotides 4313318 to 4329721
• [41] a nucleotide sequence of nucleotides 4409533 to 4423151
• [42] a nucleotide sequence of nucleotides 4496202 to 4509534
• [43] a nucleotide sequence of nucleotides 4517876 to 4599637
• (27) The E. coli mutant strain according to any one of (23) to (26) comprising chromosomal DNA that does not comprise ygiS gene, ygiT gene, ygiU gene, JW2991 gene, ygiW gene, ygiX gene, ygiY gene, ygiZ gene, mdaB gene and ygiN gene, or homologous gene thereof.
• (28) The E. coli mutant strain according to any one of (23) to (27) comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 3165628 to 3172940 of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.
• (29) The E. coli mutant strain according to any one of (23) to (28) comprising chromosomal DNA that does not comprise ybeL gene, ybeQ gene, ybeR gene, ybeS gene, ybeT gene, ybeU gene, ybeV gene, ybeW gene, ybeK gene, gltL gene, gltK gene, gltJ gene, ybeJ gene and JW0651 gene, or homologous gene thereof.
• (30) The E. coli mutant strain according to any one of (23) to (29) comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 675104 to 689374 of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.
• (31) The E. coli mutant strain according to any one of (23) to (30) comprising chromosomal DNA that does not comprise yfcC gene, JW2296 gene, yfcE gene, yfcF gene, yfcG gene, folX gene, JW2301 gene, yfcI gene, hisP gene, hisM gene, hisQ gene, hisJ gene, argT gene, yfdB gene, JW2346 gene, yfdH gene, JW2348 gene, yfdK gene, yfdL gene, yfdM gene, xylU gene, yfdN gene, yfdO gene, JW2356 gene, JW2357 gene, JW2358 gene, JW2359 gene, JW2360 gene, JW2378 gene, yqhT gene, ptwC gene and ptwB gene, or homologous gene thereof.
• (32) The E. coli mutant strain according to any one of (23) to (31) comprising chromosomal DNA that does not comprise DNA having the nucleotide sequences as shown in [1] to [3] below of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.
• [1] a nucleotide sequence of nucleotides 2423365 to 2434098
• [2] a nucleotide sequence of nucleotides 2472852 to 2482482
• [3] a nucleotide sequence of nucleotides 2505075 to 2514557
• (33) The E. coli mutant strain according to any one of (23) to (32) comprising chromosomal DNA that does not comprise pinO gene, gspA gene, gspC gene, gspD gene, gspE gene, hofF gene, hofG gene, hofH gene, gspI gene, gspJ gene, gspK gene, gspL gene, pshM gene, hofD gene, bfr gene, yheA gene and chiA gene, or homologous gene thereof.
• (34) The E. coli mutant strain according to any one of (23) to (33) comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 3453021 to 3469366 of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.
• (35) The E. coli mutant strain according to any one of (23) to (34) comprising chromosomal DNA that does not comprise elaB gene, elaA gene, elaC gene, elaD gene, yfbK gene, yfbL gene, yfbM gene, yfbN gene, JW2269 gene and JW2270 gene, or homologous gene thereof.
• (36) The E. coli mutant strain according to any one of (23) to (35) comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 2386262 to 2395471 of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.
• (37) The E. coli mutant strain according to any one of (23) to (36) comprising chromosomal DNA that does not comprise yhdJ gene, yhdU gene, envR gene, acrE gene, envD gene, yhdV gene, yhdW gene, yhdX gene and yhdY gene, or homologous gene thereof.
• (38) The E. coli mutant strain according to any one of (23) to (37) comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 3411160 to 3420878 of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto
• (39) The E. coli mutant strain according to any one of (23) to (38) comprising chromosomal DNA that does not comprise chpA gene or homologous gene thereof.
• (40) The E. coli mutant strain according to any one of (23) to (39) comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 2910274 to 2910573 of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.
• (41) The E. coli mutant strain according to any one of (23) to (40) comprising chromosomal DNA that does not comprise fhuA gene, fhuC gene, fhuD gene fhuB gene, or homologous gene thereof.

(42) The E. coli mutant strain according to any one of (23) to (41) comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 167065 to 172979 of the nucleotide sequence as shown in SEQ ID NO: 1 or DNA equivalent thereto.

TABLE 1
		Start number in SEQ ID	Stop number in SEQ ID
		NO: 1 (stop number	NO: 1 (start number when
		when encoded by	encoded by
Gene name	Coding strand	complementary strand)	complementary strand)
JW0001(thrA)		1	2463
JW0002(thrB)		2465	3397
JW0003(thrC)		3398	4684
JW0004(101#4)		4898	5194
JW0005(yaaA)	Complementary strand	5347	6123
JW0006(yaaJ)	Complementary strand	6193	7623
JW0007(talB)		7902	8855
JW0008(mog)		8970	9557
JW0009(yaaH)	Complementary strand	9592	10158
JW0010(102#2.1)	Complementary strand	10307	11020
JW0011(htgA)		10389	10979
JW0012(yaaI)	Complementary strand	11046	11450
JW0013(dnaK)		11827	13743
JW0014(dnaJ)		13832	14962
JW0015(yi81)		15109	16221
JW0016(yi82)	Complementary strand	15533	15841
JW0017(hokC)	Complementary strand	16415	16624
JW0018(nhaA)		17153	18319
JW0019(nhaR)		18379	19284
JW0020(insB)	Complementary strand	19475	19978
JW0021(insA)	Complementary strand	19897	20172
JW0022(rpsT)	Complementary strand	20479	20742
JW0023(ribF)		21071	22012
JW0024(ileS)		22055	24871
JW0025(lspA)		24871	25365
JW0026(fkpB)		25490	25939
JW0027(lytB)		25941	26891
JW0028(yaaF)		26957	27871
JW0029(dapB)		28038	28859
JW0030(carA)		29315	30463
JW0031(carB)		30481	33702
JW0032(yaaV)	Complementary strand	33750	33929
JW0033(caiF)		33964	34359
JW0034(caiE)	Complementary strand	34445	35056
JW0035(caiD)	Complementary strand	35041	35934
JW0036(caiC)	Complementary strand	35935	37503
JW0037(caiB)	Complementary strand	37562	38779
JW0038(caiA)	Complementary strand	38908	40050
JW0039(caiT)	Complementary strand	40081	41595
JW0040(fixA)		42067	42837
JW0041(fixB)		42852	43793
JW0042(fixC)		43844	45130
JW0043(fixX)		45127	45414
JW0044(yaaU)		45471	46802
JW0045(yabF)		46910	47440
JW0046(kefC)		47433	49295
JW0047(folA)		49487	49966
JW0048(apaH)	Complementary strand	50044	50886
JW0049(apaG)	Complementary strand	50893	51270
JW0050(ksgA)	Complementary strand	51273	52094
JW0051(pdxA)	Complementary strand	52091	53080
JW0052(surA)	Complementary strand	53080	54366
JW0053(imp)	Complementary strand	54419	56773
JW0054(djlA)		57028	57843
JW0055(yabP)		58138	58788
JW0056(yabQ)		58785	58943
JW0057(rluA)	Complementary strand	59351	60010
JW0058(hepA)	Complementary strand	60022	62928
JW0059(polB)	Complementary strand	63093	65444
JW0060(araD)	Complementary strand	65519	66214
JW0061(araA)	Complementary strand	66499	68001
JW0062(araB)	Complementary strand	68012	69712
JW0063(araC)		70051	70929
JW0064(yabI)		71066	71779
JW0065(yabJ)	Complementary strand	71893	72591
JW0066(yabK)	Complementary strand	72575	74185
JW0067(tbpA)	Complementary strand	74161	75144
JW0068(yabN)	Complementary strand	75308	76963
JW0069(yabM)		77285	78463
JW0070(leuD)	Complementary strand	78512	79117
JW0071(leuC)	Complementary strand	79128	80528
JW0072(leuB)	Complementary strand	80531	81622
JW0073(leuA)	Complementary strand	81622	83193
JW0074(leuL)	Complementary strand	83286	83372
JW0075(leuO)		84032	84976
JW0076(ilvI)		85294	87018
JW0077(ilvH)		87021	87512
JW0078(fruR)		87692	88696
JW0079(yabB)		89298	89756
JW0080(yabC)		89758	90699
JW0081(ftsL)		90696	91061
JW0082(ftsI)		91077	92843
JW0083(murE)		92830	94317
JW0084(murF)		94314	95672
JW0085(mraY)		95666	96748
JW0086(murD)		96751	98067
JW0087(ftsW)		98067	99311
JW0088(murG)		99308	100375
JW0089(murC)		100429	101904
JW0090(ddlB)		101897	102817
JW0091(ftsQ)		102819	103649
JW0092(ftsA)		103646	104908
JW0093(ftsZ)		104969	106120
JW0094(lpxC)		106221	107138
JW0095(yacA)		107438	107881
JW0096(secA)		107943	110648
JW0097(mutT)		110708	111097
JW0098(yacG)	Complementary strand	111313	111465
JW0099(yacF)	Complementary strand	111520	112263
JW0100(yacE)	Complementary strand	112263	112883
JW0101(guaC)		113108	114151
JW0102(hofC)	Complementary strand	114186	115388
JW0103(hofB)	Complementary strand	115378	116763
JW0104(ppdD)	Complementary strand	116773	117213
JW0105(nadC)	Complementary strand	117416	118309
JW0106(ampD)		118397	118948
JW0107(ampE)		118945	119799
JW0108(aroP)	Complementary strand	119842	121215
JW0109(pdhR)		121756	122520
JW0110(aceE)		122681	125344
JW0111(aceF)		125359	127251
JW0112(lpdA)		127576	129000
JW0113(yacH)	Complementary strand	129071	130924
JW0114(acnB)		131279	133876
JW0115(yacL)		134052	134414
JW0116(speD)	Complementary strand	134452	135246
JW0117(speE)	Complementary strand	135262	136128
JW0118(yacC)	Complementary strand	136234	136581
JW0119(yacK)		136747	138297
JW0120(gcd)	Complementary strand	138499	140889
JW0121(hpt)		141083	141631
JW0122(yadF)	Complementary strand	141672	142334
JW0123(yadG)		142443	143369
JW0124(yadH)		143366	144136
JW0125(yadI)		144241	144681
JW0126(yadJ)		144745	145974
JW0127(panD)	Complementary strand	145978	146358
JW0128(yadD)		146683	147534
JW0129(panC)	Complementary strand	147608	148459
JW0130(panB)	Complementary strand	148471	149265
JW0131(yadC)	Complementary strand	149379	150617
JW0132(yadK)	Complementary strand	150667	151263
JW0133(yadL)	Complementary strand	151290	151895
JW0134(yadM)	Complementary strand	151907	152476
JW0135(htrE)	Complementary strand	152493	155090
JW0136(ecpD)	Complementary strand	155125	155865
JW0137(yadN)	Complementary strand	155963	156547
JW0138(folK)	Complementary strand	156917	157396
JW0139(pcnB)	Complementary strand	157393	158811
JW0140(yadB)	Complementary strand	158850	159776
JW0141(dksA)	Complementary strand	159813	160268
JW0142(sfsA)	Complementary strand	160446	161150
JW0143(ligT)	Complementary strand	161165	161704
JW0144(hrpB)		161769	164198
JW0145(ponB)		164394	166928
JW0146(fhuA)		167148	169391
JW0147(fhuC)		169442	170239
JW0148(fhuD)		170239	171129
JW0149(fhuB)		171126	173108
JW0150(hemL)	Complementary strand	173266	174546
JW0151(yadQ)		174882	176192
JW0152(yadR)		176274	176618
JW0153(yadS)	Complementary strand	176665	177288
JW0154(yadT)	Complementary strand	177326	178126
JW0155(pfs)	Complementary strand	178119	178817
JW0156(dgt)		178901	180418
JW0157(htrA)		180548	181972
JW0158(yaeG)		182109	183284
JW0159(yaeH)	Complementary strand	183373	183759
JW0160(yaeI)	Complementary strand	183921	184454
JW0161(dapD)	Complementary strand	184787	185611
JW0162(glnD)	Complementary strand	185642	188314
JW0163(map)	Complementary strand	188376	189170
JW0164(rpsB)		189538	190263
JW0164.5(120#5)	Complementary strand	190215	190466
JW0165(tsf)		190521	191372
JW0166(smb)		191519	192244
JW0167(rrf)		192536	193093
JW0168(dxr)		193185	194381
JW0169(rth)		194567	195328
JW0170(cdsA)		195449	196198
JW0171(yaeL)		196210	197562
JW0172(yzzN)		197592	200024
JW0173(hlpA)		200146	200631
JW0174(lpxD)		200635	201660
JW0175(fabZ)		201765	202220
JW0176(lpxA)		202224	203012
JW0177(lpxB)		203012	204160
JW0178(rnhB)		204157	204753
JW0179(dnaE)		204790	208272
JW0180(accA)		208285	209244
JW0181(ldcC)		209343	211484
JW0182(yaeR)		211541	211930
JW0183(mesJ)		211995	213293
JW0184(rof)	Complementary strand	213342	213596
JW0185(yaeP)	Complementary strand	213589	213789
JW0186(yaeQ)		213955	214500
JW0187(yaeJ)		214497	214919
JW0188(cutF)		214933	215643
JW0189(yaeF)	Complementary strand	215843	216721
JW0190(proS)	Complementary strand	216721	218439
JW0191(yaeB)	Complementary strand	218551	219258
JW0192(rcsF)	Complementary strand	219255	219659
JW0193(yaeC)	Complementary strand	219777	220592
JW0194(yaeE)	Complementary strand	220632	221285
JW0195(abc)	Complementary strand	221278	222309
JW0196(yaeD)		222497	223072
JW0197(yafB)		228831	229634
JW0198(yafC)	Complementary strand	229631	230545
JW0199(yafD)		230807	231586
JW0200(yafE)		231590	232213
JW0201(dniR)	Complementary strand	232261	232929
JW0202(gloB)	Complementary strand	233691	234446
JW0203(yafS)		234480	235202
JW0204(rnhA)	Complementary strand	235199	235666
JW0205(dnaQ)		235731	236462
JW0206(yafT)		236999	237784
JW0207(yafU)	Complementary strand	238410	238748
JW0208(ybfL)		238854	239042
JW0209(yafV)	Complementary strand	239083	239862
JW0210(up18)		240007	240480
JW0211(yafH)	Complementary strand	240523	243045
JW0212(gmhA)		243207	243785
JW0213(yafJ)		243991	244758
JW0214(yafk)	Complementary strand	244729	245469
JW0215(yafQ)	Complementary strand	245625	245903
JW0216(dinJ)	Complementary strand	245906	246166
JW0217(yafL)		246376	247125
JW0218(yafM)		247301	247798
JW0219(fhiA)	Complementary strand	248022	249761
JW0220(lafU)		249856	250491
JW0221(dinP)		250562	251617
JW0222(yafN)		251669	251962
JW0223(yafO)		251965	252363
JW0224(yafP)		252373	252825
JW0225(127#12)		253131	253397
JW0226(prfH)		253366	253866
JW0227(pepD)	Complementary strand	253923	255380
JW0228(gpt)		255641	256099
JW0229(yafA)		256191	257435
JW0230(crl)		257493	257894
JW0231(phoE)	Complementary strand	257933	258988
JW0232(proB)		259276	260379
JW0233(proA)		260391	261644
JW0234(ykfI)	Complementary strand	262216	262557
JW0235(yafW)	Complementary strand	262578	262895
JW0235.5(yeeT)	Complementary strand	262914	263135
JW0236(ykfG)	Complementary strand	263144	263620
JW0237(yafX)	Complementary strand	263636	264268
JW0238(ykfF)	Complementary strand	264192	264593
JW0239(ykfB)	Complementary strand	264508	264975
JW0240(yafY)	Complementary strand	264998	265855
JW0241(ypjK)	Complementary strand	265441	265617
JW0242(yafZ)	Complementary strand	266072	266893
JW0243(ykfA)	Complementary strand	266985	267848
JW0244(perR)	Complementary strand	268177	269070
JW0245(129#3.1)		269166	269534
JW0246(129#3.2)		269491	270642
JW0247(129#5)		270718	271143
JW0248(ykfC)		271750	272880
JW0249(130#0.1)		272841	272951
JW0250(is5B)	Complementary strand	272989	274005
JW0251(yi51)		273325	273693
JW0252(mmuP)		274189	275616
JW0253(mmuM)		275603	276535
JW0254(afuC)	Complementary strand	276644	277690
JW0255(130#4)	Complementary strand	277702	278064
JW0256(insB)	Complementary strand	278066	278569
JW0257(insA)	Complementary strand	278488	278763
JW0258(130#5)	Complementary strand	278912	279250
JW0259(yagB)	Complementary strand	279273	279623
JW0260(yagA)	Complementary strand	279717	280871
JW0261(yagE)		281145	282074
JW0262(yagF)		282089	284056
JW0263(yagG)		284283	285665
JW0264(yagH)		285677	287287
JW0265(yagI)	Complementary strand	287292	288050
JW0266(argF)	Complementary strand	288189	289193
JW0267(yfjI)		289293	289526
JW0268(insB)	Complementary strand	289537	290040
JW0269(insA)	Complementary strand	289959	290234
JW0270(yagJ)		290388	291119
JW0271(yagK)	Complementary strand	291210	291836
JW0272(yagL)	Complementary strand	292108	292806
JW0273(yagM)	Complementary strand	292833	293687
JW0274(yagN)	Complementary strand	294027	294467
JW0275(intF)	Complementary strand	294584	295984
JW0276(yagP)	Complementary strand	296269	296679
JW0277(yagQ)	Complementary strand	296658	297614
JW0278(yagR)	Complementary strand	297624	299822
JW0279(yagS)	Complementary strand	299819	300775
JW0280(yagT)	Complementary strand	300772	301461
JW0281(yagU)		301879	302493
JW0282(ykgJ)	Complementary strand	302741	303070
JW0283(yagV)	Complementary strand	303383	304138
JW0284(yagW)	Complementary strand	304062	305705
JW0285(yagX)	Complementary strand	305695	308220
JW0286(yagY)	Complementary strand	308246	308914
JW0287(yagZ)	Complementary strand	308972	309559
JW0288(ykgK)	Complementary strand	309634	309885
JW0289(ykgL)		310994	311227
JW0290(ykgM)	Complementary strand	311402	311668
JW0291(eaeH)		313245	314132
JW0292(134#4.1)		314179	314478
JW0293(134#4.2)		314475	315341
JW0294(ykgA)	Complementary strand	315338	316057
JW0295(ykgB)	Complementary strand	316614	317216
JW0296(ykgI)	Complementary strand	317219	317470
JW0297(merA)	Complementary strand	317564	318916
JW0298(ykgD)		319115	319969
JW0299(ykgE)		320562	321215
JW0300(ykgF)		321226	322653
JW0301(ykgG)		322652	323341
JW0302(ykgH)	Complementary strand	323584	324252
JW0303(betA)	Complementary strand	324465	326135
JW0304(betB)	Complementary strand	326149	327621
JW0305(betI)	Complementary strand	327635	328222
JW0306(betT)		328351	330384
JW0307(yahA)		331259	332347
JW0308(yahB)	Complementary strand	332389	333321
JW0309(yahC)	Complementary strand	333413	333910
JW0310(yahD)		334168	334773
JW0311(yahE)		334813	335676
JW0312(yahF)		335666	337213
JW0313(yahG)		337213	338631
JW0314(yahH)		338552	338977
JW0315(arcM)		339053	340003
JW0316(yahJ)		340013	341395
JW0317(yahK)		341772	342821
JW0318(yahL)		343064	343879
JW0319(yahM)		344262	344537
JW0320(yahN)	Complementary strand	344554	345225
JW0321(yahO)		345372	345647
JW0322(prpR)	Complementary strand	345745	347331
JW0323(bcpA)		347570	348460
JW0324(prpC)		348900	350069
JW0325(prpD)		350103	351554
JW0326(prpE)		351594	353480
JW0327(codB)		353810	355069
JW0328(codA)		355059	356342
JW0329(cynR)	Complementary strand	356679	357578
JW0330(cynT)		357687	358346
JW0331(cynS)		358377	358847
JW0332(cynX)		358880	360034
JW0333(lacA)	Complementary strand	360137	360748
JW0334(lacY)	Complementary strand	360814	362067
JW0335(lacZ)	Complementary strand	362119	365193
JW0336(lacI)	Complementary strand	365316	366398
JW0337(mhpR)	Complementary strand	366475	367422
JW0338(mhpA)		367499	369163
JW0339(mhpB)		369165	370109
JW0340(mhpC)		370112	370993
JW0341(mhpD)		371003	371812
JW0342(mhpF)		371809	372759
JW0343(mhpE)		372756	373769
JW0344(mhpT)		374302	375558
JW0345(yaiL)		375660	376199
JW0346(yaiM)	Complementary strand	376423	377256
JW0347(adhC)	Complementary strand	377350	378459
JW0348(yaiN)	Complementary strand	378494	378769
JW0349(yaiO)	Complementary strand	378957	379730
JW0350(140#9)	Complementary strand	379732	379947
JW0351(yi2A)		380194	380604
JW0352(yi22)		380562	381467
JW0353(yi24)	Complementary strand	380607	381038
JW0354(yi23)		380635	380928
JW0355(yaiP)	Complementary strand	381627	382823
JW0356(yaiS)	Complementary strand	382947	383504
JW0357(tauA)		384120	385082
JW0358(tauB)		385095	385862
JW0359(tauC)		385859	386686
JW0360(ssiD)		386683	387534
JW0361(hemB)	Complementary strand	387641	388615
JW0362(yaiT)		389139	390599
JW0363(141#3.1)	Complementary strand	390627	391493
JW0364(141#3.2)	Complementary strand	391490	391789
JW0365(yaiU)		391909	393306
JW0366(yaiV)		393349	394017
JW0367(ampH)	Complementary strand	394018	395148
JW0368(sbmA)		395527	396747
JW0369(yaiW)		396760	397854
JW0370(yaiY)	Complementary strand	397913	398221
JW0371(yaiZ)		398412	398693
JW0372(ddlA)	Complementary strand	398717	399811
JW0373(yaiB)		400274	400534
JW0374(phoA)		400635	402050
JW0375(psiF)		402151	402489
JW0376(yaiC)		402591	403706
JW0377(proC)	Complementary strand	403723	404532
JW0378(yaiI)		404652	405110
JW0379(aroL)		405293	405817
JW0380(yaiA)		405867	406058
JW0381(aroM)		406316	406993
JW0382(yaiE)		407065	407349
JW0383(ykiA)		407557	407838
JW0384(yaiD)	Complementary strand	407996	408907
JW0385(yajF)		409032	409940
JW0386(araJ)	Complementary strand	410185	411369
JW0387(sbcC)	Complementary strand	411495	414641
JW0388(sbcD)	Complementary strand	414638	415840
JW0389(phoB)		416030	416719
JW0390(phoR)		416777	418072
JW0391(brnQ)		418479	419798
JW0392(proY)		419871	421247
JW0393(malZ)		421403	423220
JW0394(yajH)	Complementary strand	423225	423806
JW0395(queA)		423899	424969
JW0396(tgt)		425025	426152
JW0397(yajC)		426175	426507
JW0398(secD)		426535	428382
JW0399(secF)		428393	429364
JW0400(yajD)		429493	429840
JW0401(tsx)	Complementary strand	430017	430901
JW0402(yajI)	Complementary strand	431200	431799
JW0403(ybaD)		431890	432339
JW0404(ribD)		432343	433446
JW0405(ribH)		433535	434005
JW0406(nusB)		434025	434444
JW0407(thiL)		434522	435499
JW0408(pgpA)		435477	435995
JW0409(yajO)	Complementary strand	436049	437023
JW0410(dxs)	Complementary strand	437203	439065
JW0411(ispA)	Complementary strand	439090	439989
JW0412(xseB)	Complementary strand	439989	440231
JW0413(thiI)		440437	441885
JW0414(thiJ)	Complementary strand	441939	442535
JW0415(apbA)	Complementary strand	442492	443403
JW0416(yajQ)		443403	444062
JW0417(yajR)	Complementary strand	444190	445560
JW0418(cyoE)	Complementary strand	445703	446593
JW0419(cyoD)	Complementary strand	446605	446934
JW0420(cyoC)	Complementary strand	446934	447548
JW0421(cyoB)	Complementary strand	447538	449529
JW0422(cyoA)	Complementary strand	449551	450498
JW0423(ampG)	Complementary strand	450958	452433
JW0424(yajG)	Complementary strand	452477	453055
JW0425(bolA)		453327	453677
JW0426(tig)		454021	455319
JW0427(clpP)		455565	456188
JW0428(clpX)		456314	457588
JW0429(lon)		457776	460130
JW0430(hupB)		460339	460611
JW0431(ybaU)		460803	462674
JW0432(ybaV)		462825	463196
JW0433(ybaW)		463290	463688
JW0434(ybaX)	Complementary strand	463740	464435
JW0435(ybaE)	Complementary strand	464500	466200
JW0436(cof)		466300	467118
JW0437(ybaO)		467271	467729
JW0438(mdlA)		467759	469531
JW0439(mdlB)		469410	471305
JW0440(glnK)		471486	471824
JW0441(amtB)		471854	473140
JW0442(tesB)	Complementary strand	473189	474049
JW0443(ybaY)		474267	474839
JW0444(ybaZ)	Complementary strand	474870	475259
JW0445(ybaA)		475560	475913
JW0446(ylaB)	Complementary strand	475955	477511
JW0447(ylaC)	Complementary strand	477669	478178
JW0448(maa)	Complementary strand	478255	478806
JW0449(hha)	Complementary strand	478978	479196
JW0450(ybaJ)	Complementary strand	479222	479596
JW0451(acrB)	Complementary strand	480142	483291
JW0452(acrA)	Complementary strand	483314	484507
JW0453(acrR)		484649	485296
JW0454(aefA)		485424	488786
JW0455(ybaM)	Complementary strand	488998	489159
JW0456(priC)	Complementary strand	489173	489700
JW0457(ybaN)		489770	490147
JW0458(apt)		490300	490851
JW0459(dnaX)		490980	492911
JW0460(ybaB)		492964	493293
JW0461(recR)		493293	493898
JW0462(htpG)		494008	495882
JW0463(adk)		496063	496707
JW0464(hemH)		496943	497905
JW0465(aes)	Complementary strand	497902	498861
JW0466(gsk)		499013	500317
JW0467(ybaL)	Complementary strand	500450	502126
JW0468(fsr)	Complementary strand	502364	503584
JW0469(ushA)		503802	505454
JW0470(ybaK)	Complementary strand	505491	505970
JW0471(ybaP)	Complementary strand	506174	506968
JW0472(ybaQ)		507106	507447
JW0473(ybaR)	Complementary strand	507763	510267
JW0474(ybaS)		510529	511461
JW0475(ybaT)		511464	512756
JW0476(cueR)		512881	513288
JW0477(ybbJ)	Complementary strand	513289	513747
JW0478(ybbK)	Complementary strand	513744	514661
JW0479(ybbL)		514807	515484
JW0480(ybbM)		515444	516250
JW0481(ybbN)	Complementary strand	516313	517203
JW0482(ybbO)	Complementary strand	517228	518037
JW0483(tesA)	Complementary strand	518027	518653
JW0484(ybbA)		518621	519307
JW0485(ybbP)		519304	521718
JW0486(rhsD)		522149	526429
JW0487(ybbC)		526469	526837
JW0488(rhsE)		526837	527547
JW0489(ybbD)		527528	527788
JW0490(156#1)	Complementary strand	528533	528904
JW0491(ybbB)	Complementary strand	529020	530114
JW0492(ybbS)	Complementary strand	530183	531109
JW0493(ybbT)		531339	531821
JW0494(ybbU)		531899	532714
JW0495(gcl)		532804	534585
JW0496(gip)		534598	535374
JW0497(ybbQ)		535474	536352
JW0498(ybbV)		536384	536662
JW0499(allP)		536521	537975
JW0500(allA)		538035	539396
JW0501(ybbY)		539453	540754
JW0502(ybbZ)		540776	541921
JW0503(ylbA)	Complementary strand	542149	542934
JW0504(allC)	Complementary strand	542945	544180
JW0505(ylbC)	Complementary strand	544202	545251
JW0506(fdrA)		545568	547235
JW0507(ylbE)		547245	547505
JW0508(ylbE)		547502	548503
JW0509(ylbF)		548514	549329
JW0510(arcC)		549326	550219
JW0511(purK)	Complementary strand	550414	551481
JW0512(purE)	Complementary strand	551478	551987
JW0513(ybbF)	Complementary strand	552105	552827
JW0514(ppiB)	Complementary strand	552830	553324
JW0515(cysS)		553498	554883
JW0516(ybcI)	Complementary strand	554919	555440
JW0517(ybcJ)	Complementary strand	555548	555814
JW0518(folD)	Complementary strand	555762	556628
JW0519(sfmA)		557099	557641
JW0520(sfmC)		557861	558553
JW0521(sfmD)		558584	561187
JW0522(sfmH)		561076	562206
JW0523(sfmF)		562091	562732
JW0524(fimZ)	Complementary strand	562735	563430
JW0525(intD)	Complementary strand	563702	564865
JW0526(EXO_LAMBD)	Complementary strand	564985	565248
JW0527(VRPP_LAMBD)		565338	565574
JW0528(159#4)		565729	566028
JW0529(159#5)		566025	566891
JW0530(VREN_LAMBD)		566949	567134
JW0531(emrE)		567202	567534
JW0532(ybcK)		567789	569315
JW0533(ybcL)		569780	570331
JW0534(ybcM)		570341	571138
JW0535(ybcN)		571353	571808
JW0536(ninE)		571808	571978
JW0537(ybcO)		571971	572261
JW0538(rus)		572258	572620
JW0539(ybcQ)		572843	573226
JW0539.5(160#10)		573368	573586
JW0540(is5B)	Complementary strand	573624	574640
JW0541(yi51)		573960	574328
JW0542(nmpC)	Complementary strand	574645	575712
JW0543(vlyS)		576285	576500
JW0544(ybcS)		576500	576997
JW0545(ybcT)		576982	577455
JW0546(vboR)	Complementary strand	577487	577780
JW0547(ybcV)	Complementary strand	578071	578604
JW0548(ybcW)		578767	578973
JW0549(nohB)		579721	580266
JW0550(ybcX)		580241	580984
JW0551(ybcY)	Complementary strand	581039	581623
JW0552(161#0.2)		581762	581947
JW0553(appY)		582568	583317
JW0554(ompT)	Complementary strand	583567	584520
JW0555(envY)	Complementary strand	585034	585795
JW0556(ybcH)	Complementary strand	585978	586868
JW0557(nfrA)	Complementary strand	586869	589841
JW0558(nfrB)	Complementary strand	589828	592065
JW0559(cusS)	Complementary strand	592215	593669
JW0560(cusR)	Complementary strand	593647	594330
JW0561(cusC)		594487	595860
JW0562(ylcC)		596018	596350
JW0563(cusB)		596366	597589
JW0564(cusA)		597601	600744
JW0565(pheP)		600846	602222
JW0566(ybdG)	Complementary strand	602303	603550
JW0567(nfnB)	Complementary strand	603658	604311
JW0568(ybdF)	Complementary strand	604405	604884
JW0569(ybdJ)	Complementary strand	604838	605086
JW0570(ybdK)	Complementary strand	605152	606270
JW0571(hokE)		606624	606875
JW0572(yi81)		606952	608064
JW0573(yi82)	Complementary strand	607376	607684
JW0574(entD)	Complementary strand	608346	609116
JW0575(fepA)	Complementary strand	609141	611441
JW0576(fes)		611702	612826
JW0577(164#9)		612829	613047
JW0578(entF)		613044	616925
JW0579(fepE)		617141	618274
JW0580(fepC)	Complementary strand	618271	619086
JW0581(fepG)	Complementary strand	619083	620075
JW0582(fepD)	Complementary strand	620072	621076
JW0583(ybdA)		621187	622437
JW0584(fepB)	Complementary strand	622441	623397
JW0584.5(165#7)	Complementary strand	623401	623607
JW0585(entC)		623772	624947
JW0586(entE)		624957	626567
JW0587(entB)		626581	627438
JW0588(entA)		627438	628184
JW0589(ybdB)		628187	628600
JW0590(cstA)		628781	630886
JW0591(ybdD)		631069	631266
JW0592(ybdH)	Complementary strand	631276	632364
JW0593(ybdL)		632473	633633
JW0594(ybdm)	Complementary strand	633634	634263
JW0595(ybdN)	Complementary strand	634236	635456
JW0596(ybdO)	Complementary strand	635603	636505
JW0597(dsbG)	Complementary strand	636714	637460
JW0598(ahpC)		637832	638395
JW0599(ahpF)		638640	640205
JW0600(ybdQ)	Complementary strand	640326	640754
JW0601(ybdR)		640975	642213
JW0602(rnk)	Complementary strand	642444	642854
JW0603(rna)	Complementary strand	643084	643890
JW0604(citT)	Complementary strand	644004	645467
JW0605(citG)	Complementary strand	645518	646396
JW0606(citX)	Complementary strand	646371	646922
JW0607(citF)	Complementary strand	646926	648500
JW0608(citE)	Complementary strand	648469	649377
JW0609(citD)	Complementary strand	649374	649670
JW0610(citC)	Complementary strand	649685	650743
JW0611(citA)		651122	652780
JW0612(citB)		652749	653429
JW0613(dcuC)	Complementary strand	653470	653946
JW0614(is5B)		653909	654925
JW0615(yi51)	Complementary strand	654221	654589
JW0616(dcuC)	Complementary strand	654963	656054
JW0617(crcA)		656643	657203
JW0618(cspE)		657378	657587
JW0619(crcB)	Complementary strand	657641	658024
JW0620(ybeH)		658117	658344
JW0621(ybeM)		658341	658904
JW0622(ybeC)		659033	659236
JW0623(lipA)	Complementary strand	659337	660302
JW0624(ybeF)	Complementary strand	660511	661464
JW0625(lipB)	Complementary strand	661723	662298
JW0626(ybeD)	Complementary strand	662465	662728
JW0627(dacA)	Complementary strand	662838	664049
JW0628(rlpA)	Complementary strand	664188	665276
JW0629(mrdB)	Complementary strand	665287	666399
JW0630(mrdA)	Complementary strand	666402	668303
JW0631(ybeA)	Complementary strand	668334	668801
JW0632(ybeB)	Complementary strand	668805	669014
JW0633(phpB)	Complementary strand	669382	669993
JW0634(nadD)	Complementary strand	670017	670658
JW0635(holA)	Complementary strand	670660	671691
JW0636(rlpB)	Complementary strand	671691	672272
JW0637(leuS)	Complementary strand	672287	674869
JW0638(ybeL)		675104	675586
JW0639(ybeQ)	Complementary strand	675656	676639
JW0640(ybeR)		676797	677504
JW0641(ybeS)		677501	678928
JW0642(ybeT)	Complementary strand	678938	679492
JW0643(ybeU)		679594	680301
JW0644(ybeV)		680298	681749
JW0645(ybeW)	Complementary strand	681809	683479
JW0646(ybeK)	Complementary strand	683563	684498
JW0647(gltL)	Complementary strand	684616	685341
JW0648(gltK)	Complementary strand	685341	686015
JW0649(gltJ)	Complementary strand	686015	686755
JW0650(ybeJ)	Complementary strand	686925	687908
JW0651(171#0.1)		687884	688045
JW0652(is5B)	Complementary strand	688083	689099
JW0653(yi51)		688419	688787
JW0654(lnt)	Complementary strand	689429	690967
JW0655(ybeX)	Complementary strand	690992	691870
JW0656(ybeY)	Complementary strand	691960	692427
JW0657(phoL)	Complementary strand	692424	693503
JW0658(yleA)	Complementary strand	693617	695041
JW0659(ubiF)		695187	696362
JW0660(asnB)	Complementary strand	697599	699263
JW0661(nagD)	Complementary strand	699660	700412
JW0662(nagC)	Complementary strand	700460	701680
JW0663(nagA)	Complementary strand	701689	702837
JW0664(nagB)	Complementary strand	702897	703697
JW0665(nagE)		704030	705976
JW0666(glnS)		706179	707843
JW0667(ybfM)		708420	709826
JW0668(ybfN)		709876	710202
JW0669(fur)	Complementary strand	710286	710732
JW0670(ybfJ)		710777	711031
JW0671(fldA)	Complementary strand	711021	711551
JW0672(ybfE)	Complementary strand	711691	712053
JW0673(ybfF)	Complementary strand	712124	712888
JW0674(seqA)		713073	713618
JW0675(pgm)		713644	715284
JW0676(ybfP)		715498	715992
JW0677(ybfG)	Complementary strand	716033	716395
JW0678(ybfH)	Complementary strand	716474	716791
JW0679(potE)	Complementary strand	717032	718351
JW0680(speF)	Complementary strand	718348	720546
JW0681(ybfK)		720669	720926
JW0682(kdpE)	Complementary strand	721142	721744
JW0683(kdpD)	Complementary strand	721816	724500
JW0684(kdpC)	Complementary strand	724493	725065
JW0685(kdpB)	Complementary strand	725074	727122
JW0686(kdpA)	Complementary strand	727145	728818
JW0687(kdpF)	Complementary strand	728818	728907
JW0688(ybfA)		729220	729426
JW0689(rhsC)		729669	733862
JW0690(174#4.1)	Complementary strand	732195	732614
JW0691(ybfB)		733862	734188
JW0692(rhsA)		734306	735739
JW0693(ybfC)		735736	736305
JW0694(ybfD)		736531	736785
JW0695(ybfL)		736986	738047
JW0696(ybfD)		738178	738939
JW0697(ybgA)		739087	739596
JW0698(phrB)		739593	741011
JW0699(ybgH)	Complementary strand	741161	742642
JW0700(ybgI)		742913	743656
JW0701(ybgJ)		743679	744335
JW0702(ybgK)		744329	745261
JW0703(ybgL)		745251	745985
JW0704(end8)		746021	746812
JW0705(abrB)	Complementary strand	746809	747900
JW0706(ybgO)	Complementary strand	748007	749095
JW0707(ybgP)	Complementary strand	749065	749793
JW0708(ybgQ)	Complementary strand	749808	752306
JW0709(ybgD)	Complementary strand	752315	752881
JW0710(gltA)	Complementary strand	753271	754554
JW0711(sdhC)		755263	755652
JW0712(sdhD)		755646	755993
JW0713(sdhA)		755993	757759
JW0714(sdhB)		757775	758491
JW0715(sucA)		758792	761593
JW0716(sucB)		761608	762825
JW0717(sucC)		763100	764266
JW0718(sucD)		764266	765135
JW0719(farR)	Complementary strand	765239	765961
JW0720(hrsA)		766070	768046
JW0721(ybgB)		768064	770697
JW0722(cydA)		771544	773112
JW0723(cydB)		773128	774267
JW0724(ybgT)		774282	774395
JW0725(ybgE)		774395	774688
JW0726(ybgC)		774838	775242
JW0727(tolQ)		775239	775931
JW0728(tolR)		775935	776363
JW0729(tolA)		776428	777693
JW0730(tolB)		777823	779118
JW0731(pal)		779153	779674
JW0732(ybgF)		779684	780475
JW0733(nadA)		782171	783214
JW0734(pnuC)		783252	783971
JW0735(czcD)	Complementary strand	783968	784909
JW0736(ybgS)	Complementary strand	785023	785403
JW0737(aroG)		785719	786771
JW0738(gpmA)	Complementary strand	786929	787681
JW0739(galM)	Complementary strand	787883	788923
JW0740(galK)	Complementary strand	788917	790065
JW0741(galT)	Complementary strand	790069	791115
JW0742(galE)	Complementary strand	791125	792141
JW0743(modF)	Complementary strand	792402	793874
JW0744(modE)	Complementary strand	793942	794730
JW0745(180#2)		794853	795008
JW0746(modA)		795175	795948
JW0747(modB)		795948	796637
JW0748(modC)		796640	797698
JW0749(ybhA)	Complementary strand	797699	798517
JW0750(ybhE)		798672	799667
JW0751(ybhD)	Complementary strand	799708	800724
JW0752(ybhH)		800845	801897
JW0753(ybhI)		801973	803406
JW0754(ybhJ)		803586	805850
JW0755(ybhC)	Complementary strand	806084	807367
JW0756(ybhB)	Complementary strand	807519	807995
JW0757(bioA)	Complementary strand	808054	809343
JW0758(bioB)		809430	810470
JW0759(bioF)		810467	811621
JW0760(bioC)		811608	812363
JW0761(bioD)		812356	813033
JW0762(uvrB)		813612	815633
JW0763(ybhK)	Complementary strand	815825	816733
JW0764(moaA)		817130	818119
JW0765(moaB)		818141	818653
JW0766(moaC)		818656	819141
JW0767(moaD)		819134	819379
JW0768(moaE)		819381	819833
JW0769(ybhL)		819970	820674
JW0770(ybhM)		820879	821592
JW0771(ybhN)	Complementary strand	821628	822584
JW0772(ybhO)	Complementary strand	822584	823825
JW0773(ybhP)	Complementary strand	823822	824583
JW0774(ybhQ)		824716	825126
JW0775(ybhR)	Complementary strand	825088	825900
JW0776(ybhR)	Complementary strand	825837	826193
JW0777(ybhS)	Complementary strand	826204	827337
JW0778(yhiG)	Complementary strand	827330	829081
JW0779(yhiI)	Complementary strand	829059	830057
JW0780(ybiH)	Complementary strand	830057	830728
JW0781(rhlE)		830957	832321
JW0782(204#3.1)		832420	832566
JW0783(ybiA)	Complementary strand	832553	833035
JW0784(dinG)		833155	835305
JW0785(ybiB)		835333	836295
JW0786(ybiC)		836436	837521
JW0787(ybiJ)	Complementary strand	837750	838010
JW0788(ybiI)	Complementary strand	838275	838541
JW0789(ybiX)	Complementary strand	838615	839328
JW0790(ybiL)	Complementary strand	839334	841616
JW0791(ybiM)	Complementary strand	841881	842285
JW0792(ybiN)		842336	843343
JW0793(ybiO)	Complementary strand	843340	845700
JW0794(glnQ)	Complementary strand	845826	846548
JW0795(glnP)	Complementary strand	846545	847204
JW0796(glnH)	Complementary strand	847343	848089
JW0797(dps)	Complementary strand	848493	848996
JW0798(ybiF)	Complementary strand	849295	850182
JW0799(ompX)		850535	851050
JW0800(ybiP)	Complementary strand	851099	852682
JW0801(206#8)		853268	853735
JW0802(ybiR)		853732	854850
JW0803(yibS)	Complementary strand	854909	855829
JW0804(ybiT)		856048	857640
JW0805(ybiU)	Complementary strand	857881	859146
JW0806(ybiV)	Complementary strand	859298	860113
JW0807(ybiW)	Complementary strand	860259	862691
JW0808(ybiY)	Complementary strand	862697	863623
JW0809(mipB)		863655	864389
JW0810(moeB)	Complementary strand	864465	865214
JW0811(moeA)	Complementary strand	865214	866449
JW0812(ybiK)		866653	867618
JW0813(yliA)		867497	869476
JW0814(yliB)		869241	871034
JW0815(yliC)		871052	871972
JW0816(yliD)		871975	872886
JW0817(209#3)		873064	875412
JW0818(209#4)		875420	876748
JW0819(yliG)	Complementary strand	876795	878120
JW0820(yliH)		878333	878716
JW0821(yliI)		878827	879942
JW0822(yliJ)	Complementary strand	879939	880565
JW0823(dacC)		880812	882014
JW0824(deoR)	Complementary strand	882061	882819
JW0825(ybjG)	Complementary strand	882877	883488
JW0826(mdfA)		883758	884990
JW0827(ybjH)	Complementary strand	885031	885315
JW0828(210#3)	Complementary strand	885401	886219
JW0829(210#4)	Complementary strand	886216	887424
JW0830(210#5)		887478	888044
JW0831(210#6)	Complementary strand	888219	889904
JW0832(ybjM)		890174	890551
JW0833(grxA)	Complementary strand	890581	890838
JW0834(ybjC)		890998	891285
JW0835(mdaA)		891269	891991
JW0836(rimK)		892052	892954
JW0837(ybjN)		893042	893518
JW0838(potF)		893869	894981
JW0839(potG)		894995	896209
JW0840(potH)		896219	897172
JW0841(potI)		897169	898014
JW0842(ybjO)		898074	898562
JW0843(ybjF)		898603	899730
JW0844(artJ)	Complementary strand	899929	900660
JW0845(artM)	Complementary strand	900951	901619
JW0846(artQ)	Complementary strand	901619	902335
JW0847(artI)	Complementary strand	902342	903073
JW0848(artP)	Complementary strand	903091	903819
JW0849(ybjP)	Complementary strand	904037	904552
JW0850(211#9)		904678	905001
JW0851(211#10)		904998	905828
JW0852(211#11)	Complementary strand	905825	906874
JW0853(ybjT)	Complementary strand	906937	908442
JW0854(ltaA)	Complementary strand	908378	909379
JW0855(poxB)	Complementary strand	909416	911134
JW0856(ybjV)	Complementary strand	911267	912241
JW0857(ybjW)	Complementary strand	912247	913899
JW0858(ybjE)	Complementary strand	914043	914942
JW0859(aqpZ)	Complementary strand	915437	916132
JW0860(ybjD)		916558	918216
JW0861(ybjX)	Complementary strand	918213	919205
JW0862(213#3)		919320	920435
JW0863(ybjZ)		920432	922378
JW0864(cspD)	Complementary strand	922451	922675
JW0865(ylaJ)		922998	923318
JW0866(clpA)		923349	925625
JW0867(infA)	Complementary strand	926310	926528
JW0868(aat)	Complementary strand	926813	927517
JW0869(cydC)	Complementary strand	927559	929280
JW0870(cydD)	Complementary strand	929281	931047
JW0871(trxB)	Complementary strand	931170	932135
JW0872(lrp)		932680	933174
JW0873(ftsK)		933309	937298
JW0874(lolA)		937454	938068
JW0875(ycaJ)		938079	939422
JW0876(serS)		939513	940805
JW0877(dmsA)		941131	943488
JW0878(dmsB)		943499	944116
JW0879(dmsC)		944118	944981
JW0880(ycaC)	Complementary strand	945016	945642
JW0881(ycaD)		945956	947104
JW0882(yjeM)		947122	948744
JW0883(ycaN)	Complementary strand	948745	949653
JW0884(ycaK)		949753	950343
JW0885(pflA)	Complementary strand	950425	951165
JW0886(pflB)	Complementary strand	951357	953639
JW0887(focA)	Complementary strand	953694	954551
JW0888(ycaO)	Complementary strand	954957	956717
JW0889(ycaP)		956847	957539
JW0890(serC)		957738	958826
JW0891(aroA)		958897	960180
JW0892(ycaL)		960349	961113
JW0893(cmk)		961286	961969
JW0894(rpsA)		962080	963753
JW0895(himD)		963913	964197
JW0896(ycaI)		964327	966669
JW0897(msbA)		966706	968454
JW0898(lpxK)		968451	969437
JW0899(ycaQ)		969474	970706
JW0900(ycaR)		970758	970940
JW0901(kdsB)		970937	971683
JW0902(218#5)		971837	972730
JW0903(ycbC)	Complementary strand	972707	973486
JW0904(smtA)		973622	974407
JW0905(mukF)		974404	975726
JW0906(kicA)		975734	976411
JW0907(mukB)		976411	980871
JW0908(ycbB)		981132	982979
JW0909(ycbK)		983160	983708
JW0910(ycbL)		983735	984382
JW0911(aspC)	Complementary strand	984604	985794
JW0912(ompF)	Complementary strand	985979	987067
JW0913(asnS)	Complementary strand	987670	989070
JW0914(pncB)	Complementary strand	989239	990441
JW0915(pepN)		990707	993319
JW0916(ssuB)	Complementary strand	993362	994129
JW0917(ssuC)	Complementary strand	994126	994962
JW0918(ssuD)	Complementary strand	994928	996073
JW0919(ssuA)	Complementary strand	996070	997029
JW0920(ssuE)	Complementary strand	997022	997597
JW0921(ycbQ)		997944	998492
JW0922(ycbR)		998575	999276
JW0923(ycbS)		999301	1001901
JW0924(ycbT)		1001892	1002962
JW0925(221#10)		1002974	1003516
JW0926(221#11)		1003476	1004039
JW0927(ycbF)		1004005	1004742
JW0928(pyrD)		1004853	1005863
JW0929(ycbW)		1006001	1006579
JW0930(221#15)	Complementary strand	1006576	1007643
JW0931(ycbY)		1007929	1010037
JW0932(uup)		1010049	1011956
JW0933(pqiA)		1012086	1013339
JW0934(pqiB)		1013344	1014984
JW0935(ymbA)		1014981	1015544
JW0936(rmf)		1015800	1015967
JW0937(fabA)	Complementary strand	1016037	1016555
JW0938(222#8)	Complementary strand	1016624	1018384
JW0939(ycbG)		1018570	1019022
JW0940(ompA)	Complementary strand	1019098	1020138
JW0941(sulA)	Complementary strand	1020495	1021004
JW0942(yccR)		1021223	1021852
JW0943(yccS)	Complementary strand	1021815	1023977
JW0944(yccF)	Complementary strand	1023987	1024433
JW0945(helD)		1024556	1026610
JW0946(mgsA)	Complementary strand	1026642	1027109
JW0947(223#9)	Complementary strand	1027196	1027858
JW0948(223#10)		1027977	1028444
JW0949(yccV)	Complementary strand	1028489	1028857
JW0950(yccW)	Complementary strand	1028864	1030054
JW0951(acyP)		1029936	1030427
JW0952(yccK)	Complementary strand	1030424	1030753
JW0953(yccA)	Complementary strand	1030844	1031503
JW0953.5(224#4)	Complementary strand	1031705	1031881
JW0954(hyaA)		1032224	1033342
JW0955(hyaB)		1033339	1035132
JW0956(hyaC)		1035151	1035858
JW0957(hyaD)		1035855	1036442
JW0958(hyaE)		1036439	1036837
JW0959(hyaF)		1036834	1037691
JW0960(appC)		1037825	1039369
JW0961(appB)		1039381	1040517
JW0962(yccB)		1040530	1040622
JW0963(appA)		1040702	1042000
JW0964(yccC)	Complementary strand	1042115	1044295
JW0965(yccY)	Complementary strand	1044315	1044773
JW0966(yccZ)	Complementary strand	1044749	1045888
JW0967(ymcA)	Complementary strand	1045934	1048030
JW0968(ymcB)	Complementary strand	1048030	1048776
JW0969(ymcC)	Complementary strand	1048773	1049417
JW0970(ymcD)	Complementary strand	1049524	1049847
JW0971(insA)		1049918	1050193
JW0972(insB)		1050112	1050615
JW0973(cspH)	Complementary strand	1051048	1051248
JW0974(cspG)		1051546	1051758
JW0975(sfa)		1051932	1052162
JW0976(yccL)		1052152	1052325
JW0977(yccM)	Complementary strand	1052374	1053447
JW0978(torS)	Complementary strand	1053519	1056233
JW0979(torT)		1056346	1057374
JW0980(torR)	Complementary strand	1057347	1058039
JW0981(torC)		1058169	1059341
JW0982(torA)		1059341	1061887
JW0983(torD)		1061884	1062483
JW0984(yccD)	Complementary strand	1062635	1062940
JW0985(cbpA)	Complementary strand	1062940	1063860
JW0986(yccE)		1064121	1065377
JW0987(agp)		1065670	1066911
JW0988(yccJ)	Complementary strand	1066949	1067176
JW0989(wrbA)	Complementary strand	1067197	1067793
JW0990(yciG)		1068136	1068339
JW0991(ycdG)	Complementary strand	1068596	1069990
JW0992(228#5)	Complementary strand	1069945	1070520
JW0993(228#6)	Complementary strand	1070450	1071040
JW0994(228#7)	Complementary strand	1071050	1071850
JW0995(ycdK)	Complementary strand	1071858	1072244
JW0996(228#9)	Complementary strand	1072256	1072951
JW0997(228#10)	Complementary strand	1072948	1074096
JW0998(ycdC)		1074327	1074965
JW0999(putA)	Complementary strand	1075005	1078967
JW1000(yzpU)	Complementary strand	1079119	1079310
JW1001(putP)		1079390	1080898
JW1002(229#1)		1081539	1082270
JW1003(ycdO)		1082328	1083455
JW1004(ycdB)		1083461	1084732
JW1005(phoH)		1085077	1086141
JW1006(ycdP)	Complementary strand	1086191	1086604
JW1007(ycdQ)	Complementary strand	1086606	1087931
JW1008(ycdR)	Complementary strand	1087924	1088235
JW1009(ycdR)	Complementary strand	1088400	1089941
JW1010(ycdS)	Complementary strand	1089950	1092373
JW1011(ycdT)		1092888	1094318
JW1012(230#3)	Complementary strand	1094359	1095225
JW1013(230#3.1)	Complementary strand	1095222	1095521
JW1014(230#4)		1095607	1095930
JW1015(ycdU)		1095927	1096913
JW1016(ycdW)		1097789	1098727
JW1017(ycdX)		1098782	1099519
JW1018(ycdY)		1099543	1100097
JW1019(ycdZ)		1100151	1100690
JW1020(csgG)	Complementary strand	1100754	1101587
JW1021(csgF)	Complementary strand	1101614	1102030
JW1022(csgE)	Complementary strand	1102055	1102444
JW1023(csgD)	Complementary strand	1102449	1103099
JW1024(csgB)		1103854	1104309
JW1025(csgA)		1104350	1104805
JW1026(csgC)		1104864	1105196
JW1027(yi22)	Complementary strand	1105295	1106200
JW1028(yi24)		1105724	1106155
JW1029(yi23)	Complementary strand	1105834	1106127
JW1030(yi2A)	Complementary strand	1106158	1106568
JW1031(sfaA)		1106653	1106964
JW1032(232#3)		1107059	1107592
JW1033(ymdC)		1107534	1109015
JW1034(ymdD)	Complementary strand	1109023	1110180
JW1035(mdoG)		1110574	1112109
JW1036(232#7)	Complementary strand	1110676	1110933
JW1037(mdoH)		1112102	1114645
JW1038(yceK)		1114842	1115045
JW1039(msyB)	Complementary strand	1115046	1115420
JW1040(yceE)	Complementary strand	1115503	1116729
JW1041(htrB)	Complementary strand	1116901	1117821
JW1042(yceA)		1118046	1119098
JW1043(yceI)	Complementary strand	1119140	1119715
JW1044(233#6)	Complementary strand	1119719	1120285
JW1045(233#6.1)	Complementary strand	1120546	1120686
JW1046(solA)	Complementary strand	1120707	1121825
JW1047(yceP)	Complementary strand	1121940	1122197
JW1048(dinI)	Complementary strand	1122481	1122726
JW1049(pyrC)	Complementary strand	1122800	1123846
JW1050(yceB)	Complementary strand	1123952	1124512
JW1051(grxB)	Complementary strand	1124646	1125293
JW1052(yqjV)	Complementary strand	1125357	1126565
JW1053(rimJ)		1126801	1127385
JW1054(yceH)		1127396	1128043
JW1055(mviM)		1128045	1128968
JW1056(mviN)		1129078	1130613
JW1057(flgN)	Complementary strand	1130653	1131069
JW1058(flgM)	Complementary strand	1131074	1131367
JW1059(flgA)	Complementary strand	1131443	1132102
JW1060(flgB)		1132257	1132673
JW1061(flgC)		1132677	1133081
JW1062(flgD)		1133093	1133788
JW1063(flgE)		1133813	1135021
JW1064(flgF)		1135041	1135796
JW1065(flgG)		1135968	1136750
JW1066(flgH)		1136794	1137501
JW1067(flgI)		1137513	1138610
JW1068(flgJ)		1138610	1139551
JW1069(flgK)		1139617	1141260
JW1070(flgL)		1141272	1142225
JW1071(rne)	Complementary strand	1142421	1145606
JW1072(rluC)		1146179	1147138
JW1073(yceF)	Complementary strand	1147250	1147873
JW1074(yceD)		1148033	1148554
JW1075(rpmF)		1148606	1148779
JW1076(plsX)		1148890	1149930
JW1077(fabH)		1149998	1150951
JW1078(fabD)		1150967	1151896
JW1079(fabG)		1151909	1152643
JW1080(acpP)		1152854	1153090
JW1081(fabF)		1153178	1154419
JW1082(pabC)		1154539	1155348
JW1083(yceG)		1155351	1156373
JW1084(tmk)		1156363	1157004
JW1085(holB)		1157001	1158005
JW1086(ycfH)		1158016	1158813
JW1087(ptsG)		1159108	1160541
JW1088(fhuE)	Complementary strand	1160601	1162790
JW1089(ycfF)		1163124	1163483
JW1090(ycfL)		1163486	1163863
JW1091(ycfM)		1163874	1164518
JW1092(ycfN)		1164499	1165323
JW1093(nagZ)		1165334	1166359
JW1094(ycfP)		1166325	1166924
JW1095(ndh)		1167324	1168628
JW1096(ycfJ)		1168838	1169377
JW1097(ycfQ)	Complementary strand	1169439	1170149
JW1098(ycfR)		1170312	1170569
JW1099(ycfS)	Complementary strand	1170651	1171613
JW1100(mfd)	Complementary strand	1171757	1175203
JW1101(ycfT)	Complementary strand	1175331	1176404
JW1102(ycfU)		1176615	1177865
JW1103(ycfV)		1177720	1178559
JW1104(ycfW)		1178559	1179803
JW1105(ycfX)		1179832	1180743
JW1106(238#9)		1180759	1181598
JW1107(cobB)	Complementary strand	1181718	1182506
JW1108(ymfA)	Complementary strand	1182503	1182973
JW1109(potD)	Complementary strand	1183022	1184068
JW1110(potC)	Complementary strand	1184065	1184859
JW1111(potB)	Complementary strand	1184856	1185683
JW1112(potA)	Complementary strand	1185697	1186833
JW1113(pepT)		1187083	1188309
JW1114(ycfD)	Complementary strand	1188358	1189479
JW1115(phoQ)	Complementary strand	1189555	1191015
JW1116(phoP)	Complementary strand	1191015	1191686
JW1117(purB)	Complementary strand	1191855	1193225
JW1118(ycfC)	Complementary strand	1193229	1193876
JW1119(trmU)	Complementary strand	1193906	1195012
JW1120(ymfB)	Complementary strand	1195066	1195527
JW1121(ymfC)	Complementary strand	1195537	1196190
JW1122(icdA)		1196362	1197612
JW1123(ymfD)	Complementary strand	1198106	1198771
JW1124(ymfE)	Complementary strand	1198772	1199485
JW1125(lit)		1199934	1200827
JW1126(intE)	Complementary strand	1200918	1202045
JW1127(VXIS_BPP21)	Complementary strand	1202026	1202271
JW1128(ymfH)	Complementary strand	1202308	1202619
JW1129(ymfI)		1202691	1203077
JW1130(ymfJ)	Complementary strand	1203015	1203323
JW1131(RPC2_BPP22)	Complementary strand	1203498	1204172
JW1132(241#2)		1203960	1204463
JW1133(ymfL)		1204495	1205064
JW1134(ymfM)		1205061	1205399
JW1135(ymfN)		1205409	1206776
JW1136(ymfR)		1206788	1206970
JW1137(ymfO)		1206970	1207443
JW1138(241#8)		1207331	1208161
JW1139(241#9)		1208152	1208736
JW1140(ycfK)		1208740	1209369
JW1141(ymfS)		1209152	1209784
JW1142(ycfA)	Complementary strand	1209756	1210358
JW1143(ycfE)	Complementary strand	1210358	1210897
JW1144(pin)		1210924	1211478
JW1145(mcrA)		1211585	1212418
JW1146(241#16)		1212652	1212816
JW1147(ycgW)	Complementary strand	1212919	1213242
JW1148(ycgX)	Complementary strand	1213942	1214346
JW1149(ycgE)	Complementary strand	1214567	1215298
JW1150(242#1)	Complementary strand	1215503	1216714
JW1151(ycgZ)		1217028	1217264
JW1152(ymgA)		1217307	1217579
JW1153(242#1.4)		1217608	1217874
JW1154(ymgC)		1217987	1218235
JW1155(yahA)		1218525	1220090
JW1156(243#0.1)		1220222	1220440
JW1157(243#1)		1220840	1222360
JW1158(aidA)		1222427	1223461
JW1159(ymgD)	Complementary strand	1223544	1223879
JW1160(243#4)	Complementary strand	1223883	1224227
JW1161(243#5)		1224737	1224925
JW1162(243#5.1)		1224934	1225146
JW1163(minE)	Complementary strand	1225518	1225784
JW1164(minD)	Complementary strand	1225788	1226600
JW1165(minC)	Complementary strand	1226624	1227319
JW1166(ycgJ)		1227839	1228207
JW1167(ycgK)	Complementary strand	1228310	1228711
JW1168(ycgL)		1228920	1229246
JW1169(243#12)		1229318	1229977
JW1170(ycgN)		1230039	1230515
JW1171(clyA)	Complementary strand	1230722	1231639
JW1172(umuD)		1232006	1232425
JW1173(umuC)		1232425	1233693
JW1174(dsbB)	Complementary strand	1233739	1234275
JW1175(nhaB)	Complementary strand	1234415	1235956
JW1176(fadR)		1236177	1236896
JW1177(ycgB)	Complementary strand	1236948	1238480
JW1178(dadA)		1238810	1240108
JW1179(dadX)		1240118	1241188
JW1180(ycgO)	Complementary strand	1241574	1243184
JW1181(ldcA)	Complementary strand	1243405	1244319
JW1182(mltE)		1244305	1245030
JW1183(ycgR)	Complementary strand	1245032	1245766
JW1184(tag1)		1245967	1246221
JW1185(ycgY)		1246399	1246839
JW1186(treA)	Complementary strand	1246918	1248615
JW1187(is5B)	Complementary strand	1248821	1249837
JW1188(yi51)		1249157	1249525
JW1189(ycgC)	Complementary strand	1250134	1251555
JW1190(ycgS)	Complementary strand	1251563	1252270
JW1191(ycgT)	Complementary strand	1252206	1253306
JW1192(dhaR)		1253495	1255423
JW1193(246#4)	Complementary strand	1255523	1258390
JW1194(ychF)	Complementary strand	1259159	1260250
JW1195(pth)	Complementary strand	1260367	1260951
JW1196(ychH)		1261229	1261507
JW1197(ychM)	Complementary strand	1261562	1262887
JW1198(prsA)	Complementary strand	1263366	1264313
JW1199(ychB)	Complementary strand	1264464	1265315
JW1200(hemM)	Complementary strand	1265315	1265938
JW1201(hemA)		1266152	1267408
JW1202(prfA)		1267450	1268532
JW1203(hemK)		1268532	1269365
JW1204(248#3)		1269362	1269754
JW1205(ychA)		1269758	1270567
JW1206(kdsA)		1270603	1271457
JW1207(chaA)	Complementary strand	1273187	1274287
JW1208(chaB)		1274557	1274787
JW1209(chaC)		1274924	1275640
JW1210(ychN)	Complementary strand	1275684	1276037
JW1211(ychO)		1276363	1277616
JW1212(narL)	Complementary strand	1277617	1278267
JW1213(narX)	Complementary strand	1278260	1280056
JW1214(narK)		1280395	1281786
JW1215(narG)		1282302	1286045
JW1216(narH)		1286042	1287580
JW1217(narJ)		1287577	1288287
JW1218(narI)		1288287	1288964
JW1219(tpr)	Complementary strand	1289525	1289626
JW1220(purU)	Complementary strand	1290220	1291062
JW1221(ychJ)	Complementary strand	1291112	1291570
JW1222(ychK)		1291644	1292588
JW1223(sprE)		1292680	1293693
JW1224(galU)		1293895	1294803
JW1225(hns)	Complementary strand	1294947	1295360
JW1226(tdk)		1295965	1296582
JW1227(ychG)	Complementary strand	1296864	1297454
JW1227.5(251#6)	Complementary strand	1297406	1297636
JW1228(adhE)	Complementary strand	1297884	1300559
JW1229(ychE)		1301036	1301683
JW1230(yi2A)		1301946	1302356
JW1231(yi22)		1302314	1303219
JW1232(yi24)	Complementary strand	1302359	1302790
JW1233(yi23)		1302387	1302680
JW1234(251#8.5)	Complementary strand	1303144	1303491
JW1235(oppA)		1303757	1305388
JW1236(oppB)		1305474	1306394
JW1237(oppC)		1306409	1307317
JW1238(oppD)		1307329	1308342
JW1239(oppF)		1308339	1309343
JW1240(yciU)	Complementary strand	1309396	1309725
JW1241(cls)	Complementary strand	1309760	1311220
JW1242(kch)	Complementary strand	1311591	1312844
JW1243(yciI)	Complementary strand	1313144	1313440
JW1244(tonB)		1313649	1314383
JW1245(yciA)	Complementary strand	1314423	1314821
JW1246(yciB)	Complementary strand	1314926	1315465
JW1247(yciC)	Complementary strand	1315495	1316238
JW1248(yciD)		1316595	1317233
JW1249(yciE)	Complementary strand	1317293	1317799
JW1250(yciF)	Complementary strand	1317845	1318345
JW1251(yciG)	Complementary strand	1318431	1318610
JW1252(trpA)	Complementary strand	1318991	1319797
JW1253(trpB)	Complementary strand	1319797	1320990
JW1254(trpC)	Complementary strand	1321002	1322360
JW1255(trpD)	Complementary strand	1322364	1323959
JW1256(trpE)	Complementary strand	1323959	1325521
JW1257(trpL)	Complementary strand	1325613	1325657
JW1258(yciV)		1325795	1326676
JW1259(yciO)		1326637	1327293
JW1260(yciQ)		1327465	1329216
JW1261(yciL)		1329427	1330302
JW1262(btuR)	Complementary strand	1330342	1330932
JW1263(yciK)	Complementary strand	1330929	1331687
JW1264(sohB)		1331907	1332956
JW1265(yciN)	Complementary strand	1332992	1333243
JW1266(topA)		1333623	1336220
JW1267(cysB)		1336430	1337404
JW1268(acnA)		1338406	1341081
JW1269(ribA)	Complementary strand	1341145	1341735
JW1270(pgpB)		1341905	1342669
JW1271(yciS)		1342818	1343126
JW1272(yciM)		1343133	1344302
JW1273(pyrF)		1344496	1345233
JW1274(yciH)		1345233	1345559
JW1275(osmB)	Complementary strand	1345685	1345903
JW1276(yciT)	Complementary strand	1346172	1346921
JW1277(256#1)	Complementary strand	1347011	1347193
JW1278(yciR)	Complementary strand	1347332	1349317
JW1279(rnb)	Complementary strand	1349553	1351487
JW1280(yciW)	Complementary strand	1351555	1352760
JW1281(envM)	Complementary strand	1352826	1353614
JW1282(ycjD)	Complementary strand	1353982	1354335
JW1283(sapF)	Complementary strand	1354403	1355209
JW1284(sapD)	Complementary strand	1355211	1356203
JW1285(sapC)	Complementary strand	1356203	1357093
JW1286(sapB)	Complementary strand	1357080	1358045
JW1287(sapA)	Complementary strand	1358042	1359685
JW1288(ymjA)	Complementary strand	1359998	1360243
JW1289(yeeF)	Complementary strand	1360377	1361762
JW1290(ycjK)	Complementary strand	1362065	1363561
JW1291(ycjL)		1363695	1364459
JW1292(ycjC)		1364486	1365043
JW1293(aldH)		1365318	1366805
JW1294(ordL)		1366807	1368087
JW1295(goaG)		1368125	1369390
JW1296(pspF)	Complementary strand	1369510	1370487
JW1297(pspA)		1370654	1371322
JW1298(pspB)		1371376	1371600
JW1299(pspC)		1371600	1371959
JW1300(pspD)		1371968	1372189
JW1301(pspE)		1372264	1372578
JW1302(sucP)		1372791	1374470
JW1303(258#8)		1374484	1375776
JW1304(ycjO)		1375797	1376678
JW1305(ycjP)		1376665	1377507
JW1306(ycjQ)		1377538	1378590
JW1307(259#3)		1378600	1379397
JW1308(ycjS)		1379407	1380462
JW1309(ycjT)		1380459	1382726
JW1310(ycjU)		1382723	1383382
JW1311(ycjV)		1383396	1384364
JW1312(ompG)		1384522	1385427
JW1313(ycjW)	Complementary strand	1385538	1386536
JW1314(ycjX)		1386692	1388089
JW1315(ycjF)		1388086	1389147
JW1316(tyrR)		1389295	1390836
JW1317(tpx)	Complementary strand	1390880	1391386
JW1318(ycjG)		1391505	1392470
JW1319(ycjI)	Complementary strand	1392445	1393233
JW1319.5(260#10)	Complementary strand	1393255	1393437
JW1320(ycjY)	Complementary strand	1393508	1394440
JW1321(ycjZ)		1394566	1395465
JW1321.5(261#1.1)		1395554	1395679
JW1322(mppA)		1395802	1397415
JW1323(ynaI)	Complementary strand	1397466	1398497
JW1324(is5B)		1398651	1399667
JW1325(yi51)	Complementary strand	1398963	1399331
JW1326(ynaJ)		1399940	1400197
JW1327(ydaA)	Complementary strand	1400247	1401197
JW1328(fnr)	Complementary strand	1401349	1402101
JW1329(ogt)	Complementary strand	1402296	1402811
JW1330(ydaH)	Complementary strand	1402822	1404354
JW1331(261#9)	Complementary strand	1404385	1405830
JW1332(ydaJ)	Complementary strand	1405830	1407155
JW1333(262#1)		1407316	1408224
JW1334(ydaL)		1408554	1409117
JW1335(262#3)	Complementary strand	1409138	1410430
JW1336(262#4)		1410625	1411608
JW1336.5(262#5)		1411883	1412056
JW1337(dbpA)		1412086	1413459
JW1338(ydaO)	Complementary strand	1413588	1414523
JW1339(intR)	Complementary strand	1414575	1415810
JW1340(ydaQ)	Complementary strand	1415812	1416051
JW1341(ydaC)	Complementary strand	1416106	1416315
JW1342(lar)	Complementary strand	1416308	1416541
JW1343(recT)	Complementary strand	1416559	1417368
JW1344(recE)	Complementary strand	1417361	1419961
JW1345(racC)	Complementary strand	1420063	1420338
JW1346(ydaE)	Complementary strand	1420413	1420583
JW1347(kil)	Complementary strand	1420583	1420804
JW1348(sieB)		1421123	1421734
JW1349(ydaF)	Complementary strand	1421731	1421886
JW1350(263#6.1)	Complementary strand	1421897	1422076
JW1351(racR)	Complementary strand	1422340	1422816
JW1352(263#6.3)		1422940	1423236
JW1353(ydaT)		1423259	1423681
JW1354(ydaU)		1423694	1424551
JW1355(dnaC)		1424558	1425304
JW1356(ydaW)		1425276	1425887
JW1357(enpP)		1425914	1426219
JW1358(trkG)		1426357	1427814
JW1359(ynaK)		1427952	1428215
JW1360(ydaY)		1428196	1428555
JW1361(VMTH_LAMBD)		1429029	1430057
JW1362(VLOM_LAMBD)		1430033	1430188
JW1363(is5B)	Complementary strand	1430321	1431301
JW1364(yi51)		1430657	1431025
JW1365(VLOM_LAMBD)		1431098	1431559
JW1366(Y401_LAMBD)		1431624	1434986
JW1367(yzzL)		1434986	1435561
JW1368(tnpO)	Complementary strand	1435659	1436249
JW1369(ynaE)	Complementary strand	1436566	1436832
JW1370(upo3)	Complementary strand	1437760	1438194
JW1371(ompN)	Complementary strand	1438335	1439468
JW1372(nifJ)	Complementary strand	1439835	1443359
JW1373(ydbd)		1443744	1443899
JW1374(hslJ)	Complementary strand	1443896	1444318
JW1375(ldhA)	Complementary strand	1444429	1445418
JW1376(ydbH)		1445626	1448265
JW1377(ynbE)		1448262	1448447
JW1378(ydbL)		1448374	1448781
JW1379(feaR)	Complementary strand	1448953	1449858
JW1380(feaB)		1450094	1451593
JW1381(tynA)	Complementary strand	1451651	1453924
JW1382(paaZ)	Complementary strand	1454172	1456217
JW1383(paaA)		1456502	1457431
JW1384(paaB)		1457443	1457730
JW1385(paaC)		1457739	1458485
JW1386(paaD)		1458494	1458997
JW1387(paaE)		1459005	1460075
JW1388(paaF)		1460072	1460839
JW1389(paaG)		1460839	1461627
JW1390(paaH)		1461629	1463056
JW1391(paaI)		1463046	1463468
JW1392(paaJ)		1463468	1464673
JW1393(paaK)		1464670	1466013
JW1394(paaX)		1466114	1467064
JW1395(paaY)		1467046	1467636
JW1396(ydbA)		1467967	1470525
JW1397(yi22)	Complementary strand	1470496	1471401
JW1398(yi24)		1470925	1471356
JW1399(yi23)	Complementary strand	1471035	1471328
JW1400(yi2A)	Complementary strand	1471359	1471769
JW1401(268#0.5)		1471933	1473084
JW1402(ydbB)		1473265	1476588
JW1403(ydbC)		1476796	1477656
JW1404(ydbD)		1477713	1480025
JW1405(269#1)		1480196	1480801
JW1406(ynbB)		1480801	1481697
JW1407(269#3)		1481713	1483470
JW1408(ynbD)		1483484	1484776
JW1409(acpD)	Complementary strand	1484830	1485435
JW1410(hrpA)		1485693	1489538
JW1411(ydcF)		1489810	1490610
JW1412(aldA)		1490807	1492246
JW1413(gapC)	Complementary strand	1492288	1492539
JW1414(gapC)	Complementary strand	1492536	1492940
JW1415(cybB)		1493441	1494007
JW1416(ydcA)		1494252	1494425
JW1416.5(271#2)		1494569	1494721
JW1417(trg)		1495045	1496685
JW1418(ydcI)	Complementary strand	1496723	1497787
JW1419(271#5)		1497863	1499206
JW1420(ydcG)		1499461	1501086
JW1421(271#7)	Complementary strand	1501007	1501198
JW1422(ydcH)		1501283	1501450
JW1423(rimL)		1501513	1502052
JW1424(271#10)	Complementary strand	1502044	1503024
JW1425(tehA)		1503148	1504140
JW1426(tehB)		1504137	1504730
JW1427(272#1)		1505032	1505700
JW1427.5(272#2)	Complementary strand	1505937	1506224
JW1428(272#3)		1506292	1507440
JW1429(272#4)	Complementary strand	1507480	1508778
JW1430(272#5)		1508747	1509283
JW1431(prtC)		1509356	1511317
JW1432(yncJ)	Complementary strand	1511409	1511639
JW1433(ydcQ)		1512062	1512499
JW1434(yjiR)		1512578	1513984
JW1435(273#4)		1514229	1515374
JW1436(potA)		1515392	1516405
JW1437(273#6)		1516406	1517347
JW1438(potC)		1517337	1518131
JW1439(ydcW)		1518153	1519577
JW1440(ydcX)		1519889	1520137
JW1441(ydcY)		1520223	1520456
JW1442(ydcZ)	Complementary strand	1520457	1520906
JW1443(274#1)	Complementary strand	1520903	1521655
JW1444(yncB)		1521578	1522639
JW1445(yncC)		1522837	1523502
JW1446(yncD)	Complementary strand	1523538	1525640
JW1447(274#5)		1525882	1526943
JW1448(ansP)	Complementary strand	1527056	1528606
JW1449(275#2)		1528822	1529439
JW1450(275#2.1)		1529602	1529727
JW1451(rhsE)		1530465	1532513
JW1452(ydcD)		1532497	1532979
JW1453(yhhI)		1533161	1533907
JW1454(276#2.1)		1534026	1534151
JW1455(ydcC)		1534391	1535527
JW1456(ydcE)		1535627	1535860
JW1457(yddH)	Complementary strand	1535857	1536426
JW1458(nhoA)		1536599	1537444
JW1459(yddE)	Complementary strand	1537540	1538433
JW1460(narV)	Complementary strand	1538512	1539192
JW1461(narW)	Complementary strand	1539189	1539884
JW1462(narY)	Complementary strand	1539884	1541428
JW1463(narZ)	Complementary strand	1541425	1545165
JW1464(narU)	Complementary strand	1545247	1546635
JW1465(277#1.1)		1545457	1545627
JW1466(yddJ)	Complementary strand	1546959	1547294
JW1467(yddK)	Complementary strand	1547333	1548289
JW1468(277#2)	Complementary strand	1548313	1548603
JW1469(yddG)	Complementary strand	1548863	1549687
JW1470(fdnG)		1549976	1553023
JW1471(fdnH)		1553036	1553920
JW1472(fdnI)		1553913	1554566
JW1473(yddM)	Complementary strand	1554973	1555335
JW1474(adhP)	Complementary strand	1555403	1556413
JW1475(sfcA)	Complementary strand	1556547	1558271
JW1475.1(rpsV)	Complementary strand	1558401	1558538
JW1476(yddX)	Complementary strand	1558640	1558918
JW1477(osmC)		1559200	1559631
JW1478(yddO)	Complementary strand	1559687	1560613
JW1479(yddP)	Complementary strand	1560606	1561592
JW1480(yddQ)	Complementary strand	1561589	1562485
JW1481(yddR)	Complementary strand	1562482	1563504
JW1482(yddS)	Complementary strand	1563506	1565194
JW1483(280#1)	Complementary strand	1565070	1565651
JW1484(dos)	Complementary strand	1565909	1568308
JW1485(280#3)	Complementary strand	1568333	1569715
JW1486(280#4)	Complementary strand	1570079	1571398
JW1487(xasA)	Complementary strand	1571529	1573064
JW1488(gadB)	Complementary strand	1573220	1574620
JW1489(pqqL)	Complementary strand	1574982	1577777
JW1490(yddB)	Complementary strand	1577822	1580194
JW1491(yddA)	Complementary strand	1580232	1580963
JW1492(chuR)	Complementary strand	1582208	1583365
JW1493(301#2)	Complementary strand	1583417	1585132
JW1494(ydeO)	Complementary strand	1585501	1586262
JW1495(301#4)	Complementary strand	1586782	1589061
JW1496(fimH)	Complementary strand	1589395	1590309
JW1497(fimG)	Complementary strand	1590368	1590871
JW1498(fimF)	Complementary strand	1590884	1591414
JW1499(fimD)	Complementary strand	1591428	1592576
JW1500(hipA)	Complementary strand	1593429	1594751
JW1501(hipB)	Complementary strand	1594751	1595017
JW1502(aidA)	Complementary strand	1595240	1596640
JW1503(ydeK)	Complementary strand	1596684	1600661
JW1504(ydeV)	Complementary strand	1601192	1602784
JW1505(ydeW)	Complementary strand	1602863	1603816
JW1506(rbsA)		1604065	1605600
JW1507(ydeY)		1605594	1606622
JW1508(ydeZ)		1606622	1607614
JW1509(304#1)		1607626	1608648
JW1510(yneB)		1608675	1609550
JW1511(yneC)		1609574	1609864
JW1512(tam)		1609921	1610679
JW1513(304#5)	Complementary strand	1610683	1611648
JW1514(uxaB)	Complementary strand	1611804	1613255
JW1515(305#1)	Complementary strand	1613482	1614381
JW1516(yneG)	Complementary strand	1614541	1614900
JW1517(yneH)	Complementary strand	1614900	1615826
JW1518(yneI)	Complementary strand	1615890	1617302
JW1519(yneJ)		1617379	1618260
JW1520(305#6)		1618338	1619453
JW1521(ydeA)		1619603	1620793
JW1522(marC)	Complementary strand	1620818	1621483
JW1523(marR)		1621752	1622129
JW1524(marA)		1622143	1622532
JW1525(marB)		1622564	1622782
JW1526(ydeD)	Complementary strand	1622813	1623613
JW1527(ydeF)		1623907	1625094
JW1528(ydeH)	Complementary strand	1625535	1626425
JW1529(ydeI)	Complementary strand	1626680	1627072
JW1530(ydeJ)		1627348	1627866
JW1531(dcp)	Complementary strand	1627910	1629955
JW1532(ydfG)		1630092	1630838
JW1533(ydfH)		1630927	1631613
JW1534(ydfZ)		1631790	1631993
JW1535(ydfI)	Complementary strand	1632028	1633488
JW1536(shiA)	Complementary strand	1633577	1634860
JW1537(307#4.1)		1635614	1635880
JW1538(tnpR)		1636197	1636787
JW1539(ydfM)	Complementary strand	1636885	1637460
JW1540(Y314_LAMBD)	Complementary strand	1637460	1638422
JW1541(nohA)	Complementary strand	1638373	1638942
JW1542(ydfO)		1639607	1640032
JW1543(yccL)	Complementary strand	1640184	1640360
JW1544(cspI)	Complementary strand	1641030	1641242
JW1545(308#2)	Complementary strand	1641605	1642102
JW1546(lycV)	Complementary strand	1642099	1642632
JW1547(ydfR)	Complementary strand	1642629	1642940
JW1548(vlyS)	Complementary strand	1642945	1643235
JW1549(cspB)	Complementary strand	1643914	1644129
JW1550(cspF)		1644430	1644642
JW1551(regQ_LAMBD)	Complementary strand	1645064	1645816
JW1552(309#4)	Complementary strand	1645830	1646879
JW1553(rem)	Complementary strand	1647226	1647477
JW1554(hokD)	Complementary strand	1647694	1647849
JW1555(relE)	Complementary strand	1647921	1648208
JW1556(relB)	Complementary strand	1648208	1648447
JW1557(ydfV)		1648472	1648777
JW1558(flxA)		1648980	1649312
JW1559(ydfW)	Complementary strand	1649749	1649898
JW1560(ydfX)	Complementary strand	1649921	1650211
JW1561(dicC)	Complementary strand	1650195	1650425
JW1562(dicA)		1650509	1650916
JW1563(ydfA)		1651083	1651238
JW1564(ydfB)		1651240	1651368
JW1565(ydfC)		1651398	1651616
JW1566(dicB)		1652184	1652372
JW1567(ydfD)		1652369	1652560
JW1568(ydfE)		1652653	1653573
JW1569(yi24)	Complementary strand	1653417	1653683
JW1570(yi22)		1653456	1654112
JW1571(309#17.2)		1654087	1655283
JW1572(rspB)	Complementary strand	1655471	1656490
JW1573(rspA)	Complementary strand	1656502	1657716
JW1574(ynfA)	Complementary strand	1657922	1658248
JW1575(ynfB)		1658383	1658724
JW1576(speG)		1658759	1659319
JW1577(ynfC)	Complementary strand	1659322	1660068
JW1578(ynfD)		1660098	1660445
JW1579(ynfE)		1660644	1663070
JW1580(ynfF)		1663128	1665554
JW1581(dmsB)		1665565	1666182
JW1582(dmsC)		1665944	1667038
JW1583(ynfI)		1667072	1667695
JW1584(yadQ)		1667854	1669146
JW1585(bioD)	Complementary strand	1669099	1669806
JW1586(mlc)	Complementary strand	1669919	1671139
JW1587(ynfL)	Complementary strand	1671274	1672167
JW1588(ynfM)		1672274	1673527
JW1589(asr)		1673924	1674259
JW1590(311#13)		1674535	1675356
JW1591(312#0.1)	Complementary strand	1675395	1675724
JW1592(ebr)	Complementary strand	1675711	1676076
JW1593(yhhT)		1676488	1677522
JW1594(pntB)	Complementary strand	1677547	1678935
JW1595(pntA)	Complementary strand	1678946	1680478
JW1596(313#2)		1681002	1681946
JW1597(arcD)		1682132	1683514
JW1598(ydgB)		1683551	1684273
JW1599(ydgC)	Complementary strand	1684270	1684605
JW1600(rstA)		1684725	1685453
JW1601(rstB)		1685457	1686758
JW1602(tus)		1686834	1687763
JW1603(fumC)	Complementary strand	1687760	1689163
JW1604(fumA)	Complementary strand	1689306	1690952
JW1605(manA)		1691151	1692326
JW1606(ydgA)		1692427	1693935
JW1607(uidC)	Complementary strand	1694161	1695426
JW1608(uidB)	Complementary strand	1695465	1696838
JW1609(uidA)	Complementary strand	1696835	1698646
JW1610(uidR)	Complementary strand	1699037	1699627
JW1611(hdhA)	Complementary strand	1699848	1700615
JW1612(malI)	Complementary strand	1700727	1701755
JW1613(malX)		1701930	1703522
JW1614(malY)		1703532	1704704
JW1615(add)		1704808	1705809
JW1616(yhhX)	Complementary strand	1705843	1706922
JW1616.1(blr)		1707126	1707251
JW1617(ydgT)		1707524	1707739
JW1618(ydgK)		1707825	1708265
JW1619(ydgQ)		1708342	1708923
JW1620(ydgM)		1708923	1709501
JW1621(316#6)		1709494	1711716
JW1622(ydgO)		1711717	1712775
JW1623(ydgP)		1712779	1713399
JW1624(ydgQ)		1713403	1714098
JW1625(nth)		1714098	1714733
JW1626(ydgR)		1715344	1716846
JW1627(gst)		1716952	1717557
JW1628(pdxY)	Complementary strand	1717601	1718464
JW1629(tyrS)	Complementary strand	1718523	1719797
JW1630(pdxH)	Complementary strand	1719926	1720582
JW1631(ydhA)	Complementary strand	1720641	1720970
JW1632(ydhH)	Complementary strand	1721068	1722177
JW1633(slyB)		1722451	1722918
JW1634(slyA)	Complementary strand	1722965	1723405
JW1635(ydhI)		1723600	1723836
JW1636(yhcQ)		1723839	1724696
JW1637(yhcP)		1724696	1726708
JW1638(sodC)	Complementary strand	1726709	1727230
JW1639(ydhF)	Complementary strand	1727311	1728207
JW1640(ydhL)	Complementary strand	1728256	1728666
JW1641(ydhM)		1728682	1729197
JW1642(nemA)		1729234	1730331
JW1643(gloA)		1730412	1730819
JW1644(rnt)		1730922	1731569
JW1645(lhr)		1731662	1736278
JW1646(ydhD)	Complementary strand	1736329	1736676
JW1647(ydhO)		1737079	1737825
JW1648(sodB)		1737953	1738534
JW1649(araJ)	Complementary strand	1738696	1739865
JW1650(purR)		1740419	1741444
JW1651(ydhB)	Complementary strand	1741441	1742373
JW1652(ydhC)		1742486	1743697
JW1653(cfa)		1743988	1745136
JW1654(ribE)	Complementary strand	1745176	1745817
JW1655(ydhE)		1746032	1747405
JW1656(ag43)	Complementary strand	1747446	1748702
JW1657(319#9)		1749275	1749580
JW1658(319#10)		1749706	1751310
JW1659(319#11)	Complementary strand	1751322	1752134
JW1660(phsC)	Complementary strand	1752138	1752923
JW1661(nrfC)	Complementary strand	1752920	1753639
JW1662(319#14)	Complementary strand	1753652	1754299
JW1663(ydhV)	Complementary strand	1754303	1756489
JW1664(319#16)	Complementary strand	1756426	1757052
JW1665(319#17)	Complementary strand	1757507	1757716
JW1666(pykF)		1758273	1759685
JW1667(lpp)		1759996	1760232
JW1668(ynhG)	Complementary strand	1760296	1761300
JW1669(ynhA)	Complementary strand	1761449	1761865
JW1670(sufS)	Complementary strand	1761878	1763098
JW1671(ynhC)	Complementary strand	1763095	1764366
JW1672(ynhD)	Complementary strand	1764341	1765087
JW1673(ynhE)	Complementary strand	1765097	1766623
JW1674(ydiC)	Complementary strand	1766593	1766961
JW1675(ydiH)	Complementary strand	1767509	1767778
JW1676(ybdB)	Complementary strand	1767797	1768207
JW1677(ydiJ)	Complementary strand	1768204	1771260
JW1678(320#10)		1771649	1772761
JW1679(ydiL)		1773190	1773546
JW1680(ydiM)		1773646	1774860
JW1681(ydiN)		1775087	1776352
JW1682(ydiB)		1776364	1777230
JW1683(aroD)		1777261	1778019
JW1684(ydiF)		1778162	1779757
JW1685(ydiO)		1779717	1780922
JW1686(321#8)	Complementary strand	1780965	1781876
JW1687(ydiQ)		1782093	1782956
JW1688(ydiR)		1782976	1783914
JW1689(ydiS)		1783970	1785259
JW1690(ydiT)		1785256	1785549
JW1691(ydiD)		1785552	1787252
JW1692(ppsA)	Complementary strand	1787309	1789687
JW1693(ydiA)		1790020	1790853
JW1694(aroH)		1791010	1792056
JW1695(ydiE)		1792188	1792379
JW1696(ydiU)	Complementary strand	1792383	1793819
JW1697(323#1)	Complementary strand	1793882	1794595
JW1698(nlpC)	Complementary strand	1794842	1795306
JW1699(btuD)	Complementary strand	1795384	1796133
JW1700(btuE)	Complementary strand	1796133	1796684
JW1701(btuC)	Complementary strand	1796747	1797727
JW1702(himA)	Complementary strand	1797828	1798127
JW1703(pheT)	Complementary strand	1798132	1800519
JW1704(pheS)	Complementary strand	1800534	1801529
JW1705(pheM)	Complementary strand	1801801	1801845
JW1706(rplT)	Complementary strand	1801968	1802324
JW1707(rpmI)	Complementary strand	1802377	1802574
JW1708(infC)	Complementary strand	1802671	1803105
JW1709(thrS)	Complementary strand	1803217	1805145
JW1710(arp)		1806153	1807568
JW1711(324#3)	Complementary strand	1807900	1808658
JW1712(pfkB)		1808942	1809874
JW1713(ydiZ)		1809975	1810265
JW1714(325#1)		1810371	1811231
JW1715(yniB)	Complementary strand	1811272	1811808
JW1716(yniC)		1811955	1812623
JW1717(325#4)		1812786	1813376
JW1718(325#5)		1813509	1814900
JW1719(ydjO)	Complementary strand	1814904	1815719
JW1720(cedA)	Complementary strand	1815996	1816259
JW1721(katE)		1816442	1818703
JW1722(ydjC)	Complementary strand	1818961	1819710
JW1723(celF)	Complementary strand	1819723	1821075
JW1724(celD)	Complementary strand	1821180	1822022
JW1725(celC)	Complementary strand	1822030	1822380
JW1726(celB)	Complementary strand	1822431	1823789
JW1727(celA)	Complementary strand	1823874	1824194
JW1728(osmE)	Complementary strand	1824493	1824831
JW1729(nadE)		1825033	1825860
JW1730(327#4)		1826090	1826977
JW1731(327#5)	Complementary strand	1826937	1827512
JW1732(spy)	Complementary strand	1827715	1828200
JW1733(astE)	Complementary strand	1828530	1829498
JW1734(astB)	Complementary strand	1829491	1830834
JW1735(astD)	Complementary strand	1830831	1832366
JW1736(astA)	Complementary strand	1832306	1833340
JW1737(cstC)	Complementary strand	1833337	1834557
JW1738(xthA)		1835003	1835809
JW1739(yqeD)		1835976	1836686
JW1740(ydjY)		1836529	1837368
JW1741(ydjZ)		1837383	1838090
JW1742(ynjA)		1838090	1838638
JW1743(328#9)		1838645	1839814
JW1744(ynjC)		1840282	1841322
JW1745(328#11)		1841235	1841975
JW1746(ynjE)		1842027	1843349
JW1747(328#13)	Complementary strand	1843358	1843978
JW1748(328#14)		1844065	1844472
JW1749(328#15)	Complementary strand	1844438	1844710
JW1750(gdhA)		1844946	1846289
JW1751(329#1)	Complementary strand	1846406	1847569
JW1752(topB)	Complementary strand	1847574	1849535
JW1753(selD)	Complementary strand	1849540	1850583
JW1754(ydjA)	Complementary strand	1850700	1851251
JW1755(sppA)		1851412	1853268
JW1756(ansA)		1853435	1854451
JW1757(ydjB)		1854462	1855103
JW1758(ydjE)	Complementary strand	1855196	1856554
JW1759(ydjF)	Complementary strand	1856671	1857429
JW1760(ydjG)	Complementary strand	1857566	1858546
JW1761(330#4)	Complementary strand	1858556	1859524
JW1762(330#5)	Complementary strand	1859508	1860344
JW1763(ydjJ)	Complementary strand	1860365	1861408
JW1764(ydjK)	Complementary strand	1861425	1862732
JW1765(tdh)	Complementary strand	1862831	1863907
JW1766(330#9)	Complementary strand	1864277	1864549
JW1767(yeaA)	Complementary strand	1864591	1865004
JW1768(gapA)		1865346	1866341
JW1769(yzzQ)		1866425	1867309
JW1770(331#2)	Complementary strand	1867357	1868211
JW1771(yeaF)	Complementary strand	1868301	1869047
JW1772(yeaG)		1869483	1871417
JW1773(yeaH)		1871530	1872813
JW1774(yeaI)		1872960	1874435
JW1775(yeaJ)		1874436	1876106
JW1776(yeaK)		1876149	1876652
JW1777(332#0.1)	Complementary strand	1876653	1876787
JW1778(yeaL)		1876927	1877373
JW1179(yeaM)	Complementary strand	1877330	1878151
JW1780(yeaN)		1878248	1879429
JW1781(yeaO)		1879484	1879831
JW1782(yoaF)	Complementary strand	1879853	1880107
JW1783(332#6)		1880161	1881315
JW1784(333#1)	Complementary strand	1881582	1881830
JW1785(yoaG)	Complementary strand	1881978	1882160
JW1786(yeaR)	Complementary strand	1882164	1882523
JW1787(yeaS)	Complementary strand	1882696	1883334
JW1788(yeaT)	Complementary strand	1883461	1884384
JW1789(ttuC)		1884487	1885572
JW1790(caiT)		1885769	1887208
JW1791(yeaW)		1887120	1888364
JW1792(yeaX)		1888420	1889385
JW1793(rnd)	Complementary strand	1889439	1890566
JW1794(fadD)	Complementary strand	1890636	1892321
JW1795(slp)	Complementary strand	1892526	1893107
JW1796(yeaZ)	Complementary strand	1893147	1893842
JW1797(yoaA)	Complementary strand	1893900	1895810
JW1798(yoaB)		1895939	1896286
JW1799(334#5.1)		1896684	1897007
JW1800(yoaH)	Complementary strand	1897127	1897306
JW1801(pabB)		1897380	1898741
JW1802(yeaB)		1898745	1899323
JW1803(sdaA)		1899507	1900871
JW1804(rtn)		1901002	1902600
JW1805(yoaE)	Complementary strand	1902604	1904160
JW1806(manX)		1904623	1905594
JW1807(manY)		1905657	1906457
JW1808(manZ)		1906461	1907321
JW1809(335#5)		1907376	1907834
JW1810(yebN)		1908209	1908829
JW1811(rrmA)	Complementary strand	1908826	1909635
JW1812(cspC)	Complementary strand	1909801	1910010
JW1813(335#9.1)	Complementary strand	1910023	1910166
JW1814(335#10)	Complementary strand	1910836	1911123
JW1815(335#10.1)	Complementary strand	1911198	1911341
JW1816(kdgR)	Complementary strand	1911883	1912674
JW1817(tcmA)		1912740	1914224
JW1818(htpX)	Complementary strand	1914270	1915151
JW1819(prc)	Complementary strand	1915343	1917391
JW1820(yebJ)	Complementary strand	1917411	1917530
JW1821(336#2)	Complementary strand	1918206	1918757
JW1822(yebS)		1918833	1920116
JW1823(pqiB)		1920085	1922718
JW1824(yebU)		1922792	1924237
JW1825(336#6)		1924340	1924591
JW1826(336#7)		1924612	1924887
JW1827(pphA)	Complementary strand	1924888	1925544
JW1828(336#9)	Complementary strand	1925940	1926281
JW1829(336#10)	Complementary strand	1926294	1927166
JW1830(336#11)	Complementary strand	1927170	1927544
JW1831(holE)		1927683	1927913
JW1832(336#13)		1928015	1928671
JW1833(exoX)		1928695	1929357
JW1834(ptrB)	Complementary strand	1929354	1931414
JW1835(yebE)	Complementary strand	1931623	1932282
JW1836(yebF)	Complementary strand	1932609	1932977
JW1837(yebG)	Complementary strand	1933032	1933322
JW1838(purT)		1933456	1934634
JW1839(eda)	Complementary strand	1934690	1935331
JW1840(edd)	Complementary strand	1935368	1937179
JW1841(zwf)	Complementary strand	1937414	1938889
JW1842(yebK)		1939227	1940096
JW1843(pykA)		1940224	1941666
JW1844(mlt)	Complementary strand	1941797	1942768
JW1845(yebA)	Complementary strand	1942888	1944147
JW1846(znuA)	Complementary strand	1944226	1945212
JW1847(znuC)		1945237	1945992
JW1848(yebI)		1945989	1946774
JW1849(ruvB)	Complementary strand	1946921	1947931
JW1850(ruvA)	Complementary strand	1947940	1948551
JW1851(yebB)		1948727	1949428
JW1852(ruvC)	Complementary strand	1949430	1949951
JW1853(yebC)	Complementary strand	1949986	1950726
JW1854(ntpA)	Complementary strand	1950755	1951207
JW1855(aspS)	Complementary strand	1951325	1953097
JW1856(yecD)		1953374	1953973
JW1857(yecE)		1953970	1954788
JW1858(yecN)		1954682	1955236
JW1859(yecO)		1955277	1956020
JW1860(yecP)		1956017	1956988
JW1861(bisZ)	Complementary strand	1957153	1959582
JW1862(torY)	Complementary strand	1959607	1960707
JW1863(cutC)	Complementary strand	1961095	1961841
JW1864(yecL)	Complementary strand	1961855	1961989
JW1865(argS)		1962637	1964370
JW1866(yecT)		1964526	1965035
JW1867(flhE)	Complementary strand	1965155	1965547
JW1868(flhA)	Complementary strand	1965547	1967625
JW1869(flhB)	Complementary strand	1967618	1968766
JW1870(cheZ)	Complementary strand	1968968	1969612
JW1871(cheY)	Complementary strand	1969623	1970012
JW1872(cheB)	Complementary strand	1970027	1971076
JW1873(cheR)	Complementary strand	1971079	1971939
JW1874(tap)	Complementary strand	1971958	1973559
JW1875(tar)	Complementary strand	1973605	1975266
JW1876(cheW)	Complementary strand	1975411	1975914
JW1877(cheA)	Complementary strand	1975935	1977899
JW1878(motB)	Complementary strand	1977904	1978830
JW1879(motA)	Complementary strand	1978827	1979714
JW1880(flhC)	Complementary strand	1979841	1980419
JW1881(flhD)	Complementary strand	1980422	1980781
JW1882(340#3.1)	Complementary strand	1981216	1982232
JW1883(340#3.2)		1981552	1981920
JW1884(yecG)		1982751	1983179
JW1885(otsA)	Complementary strand	1983186	1984616
JW1886(otsB)	Complementary strand	1984585	1985385
JW1887(araH)	Complementary strand	1985552	1986541
JW1888(araG)	Complementary strand	1986553	1988067
JW1889(araF)	Complementary strand	1988137	1989126
JW1890(yecI)		1989923	1990426
JW1891(yecJ)	Complementary strand	1990505	1990756
JW1892(341#5)		1991220	1991543
JW1893(ftn)		1991714	1992211
JW1894(yecH)	Complementary strand	1992249	1992488
JW1895(tyrP)		1992679	1993890
JW1896(yecA)	Complementary strand	1993952	1994617
JW1897(pgsA)	Complementary strand	1995267	1995815
JW1898(uvrC)	Complementary strand	1995872	1997704
JW1899(uvrY)	Complementary strand	1997701	1998357
JW1900(yecF)		1998816	1999040
JW1901(sdiA)	Complementary strand	1999108	1999830
JW1902(yecC)	Complementary strand	2000060	2000812
JW1903(yecS)	Complementary strand	2000809	2001477
JW1904(yedO)	Complementary strand	2001492	2002574
JW1905(fliY)	Complementary strand	2002583	2003383
JW1906(fliZ)	Complementary strand	2003471	2004022
JW1907(fliA)	Complementary strand	2004068	2004787
JW1908(fliC)	Complementary strand	2005108	2006604
JW1909(fliD)		2006870	2008276
JW1910(fliS)		2008301	2008711
JW1911(fliT)		2008711	2009076
JW1912(amyA)		2009154	2010641
JW1913(yedD)	Complementary strand	2010675	2011088
JW1914(yedE)		2011275	2012480
JW1915(yedF)		2012477	2012710
JW1916(yedK)		2012819	2013487
JW1917(yedL)		2013598	2014077
JW1918(yedN)	Complementary strand	2014346	2014537
JW1919(yedN)	Complementary strand	2014547	2014867
JW1920(yedM)	Complementary strand	2014999	2015349
JW1921(fliE)	Complementary strand	2015698	2016012
JW1922(fliF)		2016227	2017885
JW1923(fliG)		2017878	2018873
JW1924(fliH)		2018866	2019552
JW1925(fliI)		2019552	2020925
JW1926(fliJ)		2020944	2021387
JW1927(fliK)		2021384	2022511
JW1928(fliL)		2022616	2023080
JW1929(fliM)		2023085	2024089
JW1930(fliN)		2024086	2024499
JW1931(fliO)		2024562	2024867
JW1932(fliP)		2024867	2025604
JW1933(fliQ)		2025614	2025883
JW1934(fliR)		2025891	2026676
JW1935(rcsA)		2026966	2027589
JW1936(dsrB)	Complementary strand	2027633	2027821
JW1937(344#6)		2027981	2028211
JW1938(yedP)		2028509	2029324
JW1939(344#8)	Complementary strand	2029321	2031015
JW1940(344#9)	Complementary strand	2031186	2031368
JW1941(yedI)	Complementary strand	2031447	2032364
JW1942(yedA)		2032537	2033457
JW1943(vsr)	Complementary strand	2033446	2033916
JW1944(dcm)	Complementary strand	2033897	2035315
JW1945(yedJ)	Complementary strand	2035382	2036077
JW1946(344#15)	Complementary strand	2036117	2036482
JW1947(ompC)		2037019	2037534
JW1948(344#17)		2037544	2037753
JW1949(yedS)		2037837	2038241
JW1950(yedU)		2038833	2039684
JW1951(yedV)	Complementary strand	2039792	2041150
JW1952(copR)	Complementary strand	2041150	2041932
JW1953(yedX)		2041954	2042367
JW1954(345#1)		2042476	2043480
JW1955(345#2)		2043481	2044116
JW1956(345#3)		2044373	2045023
JW1957(yodB)		2045342	2045896
JW1958(345#5)		2046649	2047446
JW1959(eaeH)		2047909	2055012
JW1960(347#0.1)	Complementary strand	2055274	2055441
JW1961(347#1)	Complementary strand	2055622	2056326
JW1962(shiA)		2056641	2057957
JW1963(amn)		2058059	2059513
JW1964(347#4)		2059856	2060572
JW1965(yeeO)	Complementary strand	2061201	2062844
JW1966(cbl)	Complementary strand	2062962	2063912
JW1967(nac)	Complementary strand	2064014	2064931
JW1968(erfK)	Complementary strand	2065389	2066321
JW1969(cobT)	Complementary strand	2066386	2067465
JW1970(cobS)	Complementary strand	2067477	2068220
JW1971(cobU)	Complementary strand	2068217	2068762
JW1972(is5B)	Complementary strand	2069303	2070319
JW1973(yi51)		2069711	2070007
JW1974(348#3)		2071621	2072025
JW1975(yi22)	Complementary strand	2071950	2072855
JW1976(yi24)		2072379	2072810
JW1977(yi23)	Complementary strand	2072489	2072782
JW1978(yi2A)	Complementary strand	2072813	2073223
JW1979(348#3.5)		2073135	2073296
JW1980(348#4)		2073242	2073502
JW1981(yeeP)		2073499	2074209
JW1982(flu)		2074537	2077656
JW1983(349#2)		2077777	2079309
JW1984(yeeS)		2079306	2079752
JW1985(yeeT)		2079815	2080036
JW1986(yeeU)		2080110	2080478
JW1987(yeeV)		2080567	2080941
JW1988(yeeW)		2080938	2081132
JW1989(yeeX)	Complementary strand	2082030	2082425
JW1990(yeeA)	Complementary strand	2082531	2083589
JW1991(gyrI)	Complementary strand	2083787	2084260
JW1992(phsE)	Complementary strand	2084379	2085551
JW1993(sbcB)		2085754	2087181
JW1994(yeeD)	Complementary strand	2087224	2087451
JW1995(yeeE)	Complementary strand	2087465	2088523
JW1996(yeeF)	Complementary strand	2088702	2090066
JW1997(yeeY)	Complementary strand	2090327	2091277
JW1998(yeeZ)	Complementary strand	2091302	2092126
JW1999(yefM)	Complementary strand	2092460	2092711
JW2000(hisL)		2092994	2093044
JW2001(hisG)		2093190	2094089
JW2002(hisD)		2094095	2095399
JW2003(hisC)		2095396	2096466
JW2004(hisB)		2096466	2097533
JW2005(hisH)		2097533	2098123
JW2006(hisA)		2098123	2098860
JW2007(hisF)		2098842	2099618
JW2008(hisI)		2099612	2100223
JW2009(wzz)	Complementary strand	2100319	2101299
JW2010(ugd)	Complementary strand	2101445	2102611
JW2011(gnd)	Complementary strand	2102860	2104266
JW2012(yefJ)	Complementary strand	2104394	2104675
JW2013(351#10)		2104805	2104855
JW2014(is5B)	Complementary strand	2104893	2105909
JW2015(yi51)		2105229	2105597
JW2016(yefJ)	Complementary strand	2105914	2106387
JW2017(yefI)	Complementary strand	2106389	2107507
JW2018(wbbJ)	Complementary strand	2107492	2108082
JW2019(yefG)	Complementary strand	2108063	2109055
JW2020(rfc)	Complementary strand	2109058	2110224
JW2021(glf)	Complementary strand	2110224	2111327
JW2022(rfbX)	Complementary strand	2111335	2112582
JW2023(rfbC)	Complementary strand	2112579	2113136
JW2024(rfbA)	Complementary strand	2113136	2114017
JW2025(rfbD)	Complementary strand	2114075	2114974
JW2026(rfbB)	Complementary strand	2114974	2116059
JW2027(galF)	Complementary strand	2116432	2117325
JW2028(wcaM)	Complementary strand	2117500	2118894
JW2029(wcaL)	Complementary strand	2118905	2120125
JW2030(wcaK)	Complementary strand	2120122	2121402
JW2031(wzxC)	Complementary strand	2121678	2123156
JW2032(wcaJ)	Complementary strand	2123158	2124552
JW2033(cpsG)	Complementary strand	2124607	2125977
JW2034(cpsB)	Complementary strand	2126082	2127518
JW2035(wcaI)	Complementary strand	2127521	2128744
JW2036(wcaH)	Complementary strand	2128741	2129223
JW2037(wcaG)	Complementary strand	2129223	2130188
JW2038(gmd)	Complementary strand	2130191	2131312
JW2039(wcaF)	Complementary strand	2131338	2131886
JW2040(wcaE)	Complementary strand	2131902	2132648
JW2041(wcaD)	Complementary strand	2132659	2133876
JW2042(wcaC)	Complementary strand	2133851	2135068
JW2043(wcaB)	Complementary strand	2135065	2135553
JW2044(wcaA)	Complementary strand	2135556	2136395
JW2045(yccC)	Complementary strand	2136488	2138650
JW2046(wzb)	Complementary strand	2138653	2139096
JW2047(wza)	Complementary strand	2139102	2140241
JW2048(yegH)		2140861	2142483
JW2049(asmA)	Complementary strand	2142757	2144610
JW2050(dcd)	Complementary strand	2144632	2145213
JW2051(udk)	Complementary strand	2145305	2145946
JW2052(yegE)		2146264	2149581
JW2053(alkA)	Complementary strand	2149690	2150538
JW2054(yegD)		2150672	2152024
JW2055(356#6)	Complementary strand	2152037	2153983
JW2056(356#7)		2154183	2154644
JW2057(356#8)	Complementary strand	2154709	2155470
JW2058(356#9)	Complementary strand	2155467	2156126
JW2059(yegM)		2156894	2158261
JW2060(yegN)		2158261	2161383
JW2061(yegO)		2161384	2164461
JW2062(yegB)		2164462	2165877
JW2063(baeS)		2165874	2167277
JW2064(baeR)		2167274	2167996
JW2065(358#3)		2168148	2168519
JW2066(yegQ)		2168666	2170027
JW2067(ogrK)	Complementary strand	2170300	2170518
JW2068(358#6)	Complementary strand	2170600	2170746
JW2069(358#6.1)	Complementary strand	2170987	2171364
JW2070(yegS)		2171710	2172609
JW2071(gatR)	Complementary strand	2172691	2173164
JW2072(358#8.1)		2173234	2173533
JW2073(358#8.2)		2173530	2174396
JW2074(gatR)	Complementary strand	2174393	2174731
JW2075(gatD)	Complementary strand	2174831	2175871
JW2076(gatC)	Complementary strand	2175919	2177274
JW2077(gatB)	Complementary strand	2177278	2177562
JW2078(gatA)	Complementary strand	2177593	2177913
JW2079(is5B)		2177876	2178892
JW2080(yi51)	Complementary strand	2178188	2178556
JW2081(gatA)	Complementary strand	2178930	2179244
JW2082(gatZ)	Complementary strand	2179254	2180516
JW2083(gatY)	Complementary strand	2180545	2181405
JW2084(fbaB)	Complementary strand	2181707	2182831
JW2085(xegT)		2183016	2184293
JW2086(yegU)		2184290	2185294
JW2087(yegV)		2185291	2186256
JW2088(yegW)	Complementary strand	2186230	2186976
JW2089(yegX)	Complementary strand	2187028	2187855
JW2090(thiD)	Complementary strand	2187911	2188711
JW2091(thiM)	Complementary strand	2188708	2189496
JW2092(yohL)	Complementary strand	2189719	2189991
JW2093(yohM)		2190112	2190936
JW2094(360#4)		2190975	2191493
JW2095(yehA)	Complementary strand	2191575	2192609
JW2096(yehB)	Complementary strand	2192625	2195105
JW2097(yehC)	Complementary strand	2195121	2195840
JW2098(yehD)	Complementary strand	2195875	2196417
JW2099(yehE)	Complementary strand	2196710	2196991
JW2100(mrp)	Complementary strand	2197254	2198363
JW2101(metG)		2198495	2200528
JW2102(molR)		2200669	2201493
JW2103(molR)		2201950	2203542
JW2104(molR)		2204165	2204464
JW2105(yehI)		2204474	2208106
JW2106(yehK)		2208167	2208484
JW2107(yehL)		2208725	2209879
JW2108(yehO)		2209890	2212169
JW2109(yehP)		2211934	2213298
JW2110(yehQ)		2213295	2215139
JW2111(yehR)		2215408	2215881
JW2112(yehS)	Complementary strand	2215921	2216391
JW2113(yehT)	Complementary strand	2216438	2217172
JW2114(yehU)	Complementary strand	2217154	2218854
JW2115(yehV)		2219061	2219792
JW2116(yehW)	Complementary strand	2219940	2220671
JW2117(yehX)	Complementary strand	2220676	2221602
JW2118(yehY)	Complementary strand	2221595	2222752
JW2119(yehZ)	Complementary strand	2222759	2223676
JW2120(bglX)	Complementary strand	2223887	2226184
JW2121(dld)		2226380	2228095
JW2122(pbpG)	Complementary strand	2228133	2229074
JW2123(yohC)	Complementary strand	2229239	2229850
JW2124(yohD)		2229996	2230574
JW2125(yohF)	Complementary strand	2230704	2231465
JW2126(yohG)	Complementary strand	2231518	2232714
JW2127(yohH)	Complementary strand	2232744	2233034
JW2128(yohI)	Complementary strand	2233633	2234580
JW2129(yohJ)		2234819	2235217
JW2130(yohK)		2235214	2235909
JW2131(cdd)		2236039	2236923
JW2132(sanA)		2237073	2237792
JW2133(gltD)		2238228	2239466
JW2134(yeiA)		2239460	2240695
JW2135(mglC)	Complementary strand	2240938	2241948
JW2136(mglA)	Complementary strand	2241964	2243484
JW2137(mglB)	Complementary strand	2243545	2244543
JW2138(galS)	Complementary strand	2244823	2245863
JW2139(yeiB)	Complementary strand	2246005	2247162
JW2140(folE)	Complementary strand	2247179	2247847
JW2141(yeiG)		2248105	2248941
JW2142(cirA)	Complementary strand	2248973	2250964
JW2143(lysP)	Complementary strand	2251258	2252727
JW2144(yeiE)	Complementary strand	2252932	2253813
JW2145(yeiH)		2253912	2254961
JW2146(nfo)		2255035	2255892
JW2147(yeiI)		2255895	2256983
JW2148(yeiJ)	Complementary strand	2257090	2258340
JW2149(yeiK)	Complementary strand	2258440	2259381
JW2150(yeiL)		2259550	2260209
JW2151(yeiM)	Complementary strand	2260280	2261530
JW2152(yeiN)	Complementary strand	2261624	2262562
JW2153(yeiC)	Complementary strand	2262550	2263491
JW2154(fruA)	Complementary strand	2263914	2265605
JW2155(fruK)	Complementary strand	2265622	2266560
JW2156(fruB)	Complementary strand	2266560	2267690
JW2157(yeiO)		2268058	2269239
JW2158(369#3.1)	Complementary strand	2269236	2269622
JW2159(yeiP)		2269684	2270217
JW2160(yeiQ)		2270440	2271906
JW2161(yeiR)		2272024	2273010
JW2162(yeiU)		2273049	2273762
JW2163(spr)		2274174	2274740
JW2164(rtn)		2274921	2276477
JW2165(yejA)		2276559	2278373
JW2166(yejB)		2278374	2279468
JW2167(yejE)		2279468	2280493
JW2168(yejF)		2280495	2282084
JW2169(yejG)	Complementary strand	2282088	2282432
JW2170(bcr)	Complementary strand	2282765	2283898
JW2171(rsuA)	Complementary strand	2283983	2284678
JW2172(yejH)		2284827	2286587
JW2173(rplY)		2286712	2286996
JW2174(yejK)	Complementary strand	2287135	2288142
JW2175(yejL)		2288324	2288551
JW2176(yejM)		2288571	2290331
JW2177(yejO)	Complementary strand	2290585	2292807
JW2178(371#9)		2293100	2293222
JW2179(is5B)	Complementary strand	2293260	2294276
JW2180(yi51)		2293596	2293964
JW2181(narP)		2294695	2295342
JW2182(ccmH)	Complementary strand	2295553	2296605
JW2183(dsbE)	Complementary strand	2296602	2297159
JW2184(ccmF)	Complementary strand	2297156	2299099
JW2185(ccmE)	Complementary strand	2299096	2299575
JW2186(ccmD)	Complementary strand	2299572	2299781
JW2187(ccmC)	Complementary strand	2299778	2300515
JW2188(ccmB)	Complementary strand	2300557	2301219
JW2189(ccmA)	Complementary strand	2301216	2301833
JW2190(napC)	Complementary strand	2301852	2302454
JW2191(napB)	Complementary strand	2302464	2302934
JW2192(napH)	Complementary strand	2302910	2303773
JW2193(napG)	Complementary strand	2303760	2304455
JW2194(napA)	Complementary strand	2304462	2306948
JW2195(napD)	Complementary strand	2306945	2307208
JW2196(napF)	Complementary strand	2307198	2307692
JW2197(eco)		2308100	2308588
JW2198(yojH)	Complementary strand	2309303	2310949
JW2199(yojI)	Complementary strand	2311167	2312810
JW2200(alkB)	Complementary strand	2312886	2313536
JW2201(ada)	Complementary strand	2313536	2314600
JW2202(apbE)	Complementary strand	2314674	2315276
JW2203(ompC)	Complementary strand	2315841	2316944
JW2204(yojN)		2317683	2320355
JW2205(rcsB)		2320372	2321022
JW2206(rcsC)	Complementary strand	2321222	2321599
JW2207(374#2.1)		2321622	2321741
JW2208(yi22)	Complementary strand	2321666	2322571
JW2209(yi24)		2322095	2322526
JW2210(yi23)	Complementary strand	2322205	2322498
JW2211(yi2A)	Complementary strand	2322529	2322939
JW2212(rcsC)	Complementary strand	2322936	2325359
JW2213(atoS)		2325574	2327400
JW2214(atoC)		2327397	2328782
JW2215(atoD)		2328978	2329640
JW2216(atoA)		2329640	2330290
JW2217(atoE)		2330287	2331609
JW2218(atoB)		2331640	2332824
JW2219(376#1)	Complementary strand	2332898	2333674
JW2220(376#2)	Complementary strand	2333679	2335328
JW2221(376#3)	Complementary strand	2335329	2335814
JW2222(376#4)	Complementary strand	2335830	2339933
JW2223(376#5)	Complementary strand	2339867	2340490
JW2224(yfaA)	Complementary strand	2340487	2342175
JW2225(gyrA)	Complementary strand	2342324	2344951
JW2226(ubiG)		2345098	2345820
JW2227(yfaL)	Complementary strand	2345948	2349700
JW2228(nrdA)		2350396	2352681
JW2229(nrdB)		2352915	2354045
JW2230(yfaE)		2354045	2354299
JW2231(inaA)	Complementary strand	2354353	2355003
JW2232(yfaH)		2355218	2355424
JW2233(glpQ)	Complementary strand	2355466	2356542
JW2234(glpT)	Complementary strand	2356547	2357905
JW2235(glpA)		2358178	2359806
JW2236(glpB)		2359796	2361055
JW2237(glpC)		2361052	2362242
JW2238(yfaD)		2362435	2363334
JW2238.5(379#2)		2363347	2363532
JW2239(yhaF)	Complementary strand	2363573	2364376
JW2240(379#4)	Complementary strand	2364394	2365683
JW2241(379#5)	Complementary strand	2365740	2366957
JW2242(yjhI)	Complementary strand	2366960	2367742
JW2243(cinA)	Complementary strand	2367962	2369164
JW2244(379#8)	Complementary strand	2369264	2369773
JW2245(yfaO)		2370085	2370510
JW2246(ais)	Complementary strand	2370549	2371151
JW2247(ybfE)		2371426	2372598
JW2248(yfbF)		2372602	2373570
JW2249(yfbG)		2373570	2375552
JW2250(380#2)		2375549	2376439
JW2251(380#3)		2376439	2378091
JW2252(380#4)		2378088	2378423
JW2253(380#5)		2378141	2378809
JW2254(pmrD)	Complementary strand	2378803	2379069
JW2255(menE)	Complementary strand	2379179	2380534
JW2256(menC)	Complementary strand	2380531	2381493
JW2257(menB)	Complementary strand	2381493	2382350
JW2258(yfbB)	Complementary strand	2382365	2383123
JW2259(menD)	Complementary strand	2383120	2384994
JW2260(menF)	Complementary strand	2384879	2386174
JW2261(elaB)	Complementary strand	2386253	2386558
JW2262(elaA)	Complementary strand	2386613	2387074
JW2263(elaC)		2387139	2388056
JW2264(elaD)		2388247	2389455
JW2265(yfbK)	Complementary strand	2389526	2391253
JW2266(yfbL)		2391391	2392362
JW2267(yfbM)		2392465	2392968
JW2268(yfbN)	Complementary strand	2393241	2393957
JW2269(403#0.1)		2394112	2394588
JW2270(403#1)		2394644	2395495
JW2271(nuoN)	Complementary strand	2395579	2396856
JW2272(nuoM)	Complementary strand	2397043	2398572
JW2273(nuoL)	Complementary strand	2398736	2400577
JW2274(nuoK)	Complementary strand	2400574	2400876
JW2275(nuoJ)	Complementary strand	2400873	2401427
JW2276(nuoI)	Complementary strand	2401439	2401981
JW2277(nuoH)	Complementary strand	2401996	2402973
JW2278(nuoG)	Complementary strand	2402970	2405702
JW2279(nuoF)	Complementary strand	2405749	2407086
JW2280(nuoE)	Complementary strand	2407083	2407583
JW2281(nuoD)	Complementary strand	2407586	2408809
JW2282(nuoB)	Complementary strand	2409482	2410144
JW2283(nuoA)	Complementary strand	2410160	2410603
JW2284(lrhA)	Complementary strand	2411234	2412172
JW2285(insB)	Complementary strand	2412334	2412837
JW2286(insA)	Complementary strand	2412756	2413031
JW2287(yfbQ)		2413868	2415085
JW2288(404#8)		2415169	2415768
JW2289(yfbS)	Complementary strand	2415827	2417659
JW2290(yfbT)	Complementary strand	2417746	2418414
JW2291(yfbU)	Complementary strand	2418407	2418901
JW2292(405#4)	Complementary strand	2418984	2419439
JW2293(ackA)		2419777	2420979
JW2294(pta)		2421054	2423198
JW2295(yfcC)		2423388	2424908
JW2296(406#2)	Complementary strand	2424941	2425483
JW2297(yfcE)	Complementary strand	2425541	2426092
JW2298(yfcF)	Complementary strand	2426148	2426792
JW2299(yfcG)		2426928	2427575
JW2300(folX)		2427632	2427994
JW2301(406#7)		2428015	2428908
JW2302(yfcI)	Complementary strand	2428956	2429846
JW2303(hisP)	Complementary strand	2430043	2430816
JW2304(hisM)	Complementary strand	2430824	2431540
JW2305(hisQ)	Complementary strand	2431537	2432223
JW2306(hisJ)	Complementary strand	2432313	2433095
JW2307(argT)	Complementary strand	2433316	2434098
JW2308(ubiX)	Complementary strand	2434364	2434933
JW2309(purF)	Complementary strand	2435028	2436545
JW2310(dedE)	Complementary strand	2436582	2437070
JW2311(dedD)	Complementary strand	2437329	2437964
JW2312(folC)	Complementary strand	2437981	2439249
JW2313(accD)	Complementary strand	2439319	2440233
JW2314(dedA)	Complementary strand	2440389	2441048
JW2315(truA)	Complementary strand	2441131	2441943
JW2316(usg1)	Complementary strand	2441943	2442956
JW2317(pdxB)	Complementary strand	2443022	2444158
JW2318(div)		2444257	2445252
JW2319(yhhS)	Complementary strand	2445249	2446427
JW2320(fabB)	Complementary strand	2446692	2447912
JW2321(ddpX)		2448011	2450077
JW2322(408#3)	Complementary strand	2450198	2450476
JW2323(yfcM)	Complementary strand	2450510	2451244
JW2324(yfcA)	Complementary strand	2451058	2451867
JW2325(mepA)	Complementary strand	2451867	2452691
JW2326(aroC)	Complementary strand	2452695	2453780
JW2327(yfcB)	Complementary strand	2453815	2455080
JW2328(yfcN)		2454913	2455464
JW2329(409#4)	Complementary strand	2455535	2456356
JW2330(409#5)	Complementary strand	2456358	2456897
JW2331(409#6)	Complementary strand	2456894	2457382
JW2332(409#7)	Complementary strand	2457379	2457891
JW2333(yfcS)	Complementary strand	2457891	2458643
JW2334(yfcU)	Complementary strand	2458663	2459559
JW2335(yfcU)	Complementary strand	2459572	2461308
JW2336(409#10)	Complementary strand	2461390	2461953
JW2337(sixA)	Complementary strand	2462634	2463119
JW2338(yfcX)	Complementary strand	2463322	2465466
JW2339(yfcY)	Complementary strand	2465466	2466776
JW2340(yfcZ)	Complementary strand	2466957	2467241
JW2341(fadL)		2467607	2468953
JW2342(410#5.1)		2469319	2470377
JW2343(vacJ)	Complementary strand	2470559	2471314
JW2344(yfdC)		2471608	2472540
JW2345(yfdB)		2472852	2474009
JW2346(411#1)		2474162	2474524
JW2347(yfdH)		2474521	2475441
JW2348(411#2.1)	Complementary strand	2475431	2475565
JW2349(ycfA)		2477068	2477412
JW2350(yfdK)	Complementary strand	2477384	2477824
JW2351(yfdL)	Complementary strand	2477851	2478543
JW2352(yfdM)	Complementary strand	2478419	2478694
JW2353(xylU)		2478738	2478923
JW2354(yfdN)	Complementary strand	2478694	2479182
JW2355(yfdO)	Complementary strand	2479185	2479553
JW2356(411#10)		2479827	2480273
JW2357(411#11)		2480339	2481163
JW2358(411#12)		2481291	2481827
JW2359(411#13)		2481818	2482180
JW2360(411#14)		2482180	2482485
JW2361(dsdC)	Complementary strand	2483001	2483936
JW2362(dsdX)		2484154	2485491
JW2363(dsdA)		2485509	2486837
JW2364(emrY)	Complementary strand	2486945	2488483
JW2365(emrK)	Complementary strand	2488483	2489646
JW2366(evgA)		2490062	2490676
JW2367(evgS)		2490681	2494274
JW2368(bifF)	Complementary strand	2494330	2495475
JW2369(413#2.1)	Complementary strand	2495549	2496493
JW2370(yfdU)	Complementary strand	2496563	2498257
JW2371(414#1)	Complementary strand	2498311	2499561
JW2372(414#2)	Complementary strand	2500074	2500709
JW2373(ypdI)		2501005	2501280
JW2374(414#3)	Complementary strand	2501357	2501599
JW2375(ddg)		2501952	2502872
JW2376(yfdZ)	Complementary strand	2503364	2504602
JW2377(yehU)		2504996	2506675
JW2378(414#7)		2506690	2507424
JW2379(yijO)		2507437	2508294
JW2380(ptsA)	Complementary strand	2508297	2510792
JW2381(frvX)	Complementary strand	2510817	2511854
JW2382(yqhT)	Complementary strand	2511854	2512939
JW2383(ptwC)	Complementary strand	2512954	2514201
JW2384(ptwB)	Complementary strand	2514223	2514600
JW2385(glk)	Complementary strand	2514768	2515733
JW2386(416#3)		2515937	2517193
JW2387(416#4)		2517308	2517634
JW2388(mntH)	Complementary strand	2517775	2519013
JW2389(nupC)		2519349	2520551
JW2390(yi81)		2520638	2521750
JW2391(yi82)	Complementary strand	2521062	2521370
JW2392(yffA)	Complementary strand	2521950	2524199
JW2393(yfeC)		2524774	2525118
JW2394(yfeD)		2525120	2525512
JW2395(gltX)	Complementary strand	2525564	2526979
JW2396(xapR)	Complementary strand	2527900	2528784
JW2397(xapB)	Complementary strand	2529036	2530292
JW2398(xapA)	Complementary strand	2530352	2531185
JW2399(yfeN)		2531434	2532198
JW2400(yfeR)	Complementary strand	2532237	2533163
JW2401(yfeH)		2533253	2534299
JW2402(417#10)	Complementary strand	2534318	2534557
JW2403(lig)	Complementary strand	2534466	2536481
JW2404(zipA)	Complementary strand	2536552	2537538
JW2405(417#12.1)		2537070	2537312
JW2406(cysZ)		2537769	2538530
JW2407(cysK)		2538715	2539686
JW2408(ptsH)		2540070	2540327
JW2409(ptsI)		2540372	2542099
JW2410(gsr)		2542140	2542649
JW2411(pdxK)	Complementary strand	2542692	2543543
JW2412(yfeK)		2543648	2544022
JW2413(419#2)		2544055	2544789
JW2414(cysM)	Complementary strand	2544978	2545889
JW2415(cysA)	Complementary strand	2546023	2547120
JW2416(cysW)	Complementary strand	2547110	2547985
JW2417(cysU)	Complementary strand	2547985	2548818
JW2418(cysP)	Complementary strand	2548818	2549834
JW2419(ucpA)	Complementary strand	2550138	2550995
JW2420(yfeT)	Complementary strand	2551058	2551915
JW2421(yfeU)		2552079	2552975
JW2422(ptbA)		2552979	2554403
JW2423(yfeW)		2554321	2555712
JW2424(420#4)	Complementary strand	2555952	2556851
JW2425(420#5)	Complementary strand	2556947	2557522
JW2426(yfeZ)	Complementary strand	2557583	2558032
JW2427(420#7)	Complementary strand	2558019	2558444
JW2428(amiA)		2558658	2559527
JW2429(hemF)		2559531	2560430
JW2430(yfeG)	Complementary strand	2560436	2561488
JW2431(cchA)	Complementary strand	2561534	2562034
JW2432(421#4)	Complementary strand	2562047	2562706
JW2433(eutC)	Complementary strand	2562716	2563603
JW2434(eutB)	Complementary strand	2563624	2564985
JW2435(421#7)	Complementary strand	2564997	2566400
JW2436(eutH)	Complementary strand	2566397	2567623
JW2437(eutG)	Complementary strand	2567840	2569027
JW2438(eutJ)	Complementary strand	2569017	2569853
JW2439(eutE)	Complementary strand	2569864	2571267
JW2440(cchB)	Complementary strand	2571279	2571566
JW2441(cchA)	Complementary strand	2571673	2571966
JW2442(eutI)	Complementary strand	2572005	2573021
JW2443(421#15)	Complementary strand	2573018	2573821
JW2444(421#16)	Complementary strand	2573818	2574519
JW2445(421#17)	Complementary strand	2574494	2574973
JW2446(ypfE)	Complementary strand	2574986	2575321
JW2447(421#19)	Complementary strand	2575614	2577893
JW2448(talA)		2578182	2579132
JW2449(tktB)		2579152	2581155
JW2450(ypfG)	Complementary strand	2581250	2582293
JW2451(yffH)	Complementary strand	2582419	2582994
JW2452(aegA)	Complementary strand	2583062	2585041
JW2453(narQ)		2585247	2586947
JW2454(acrD)		2587111	2590224
JW2455(yffB)		2590763	2591119
JW2456(dapE)		2591123	2592250
JW2457(423#3)		2592278	2592478
JW2458(ypfH)	Complementary strand	2592588	2593310
JW2459(ypfI)	Complementary strand	2593360	2595375
JW2460(423#6)	Complementary strand	2595390	2596253
JW2461(purC)	Complementary strand	2596421	2597134
JW2462(nlpB)	Complementary strand	2597347	2598381
JW2463(dapA)	Complementary strand	2598398	2599276
JW2464(gcvR)		2599422	2599994
JW2465(bcp)		2599994	2600464
JW2466(hyfA)		2600717	2601334
JW2467(hyfB)		2601334	2603352
JW2468(hyfC)		2603363	2604310
JW2469(hyfD)		2604327	2605766
JW2470(hyfE)		2605778	2606428
JW2471(hyfF)		2606433	2608013
JW2472(hyfG)		2608003	2609670
JW2473(hyfH)		2609680	2610225
JW2474(hyfI)		2610222	2610977
JW2475(hyfJ)		2610974	2611387
JW2476(hyfR)		2611417	2613429
JW2477(focB)		2613451	2614299
JW2478(perm)	Complementary strand	2614337	2615398
JW2479(426#2.1)		2615611	2617074
JW2480(yfgD)		2617095	2617454
JW2481(yfgE)	Complementary strand	2617592	2618185
JW2482(uraA)	Complementary strand	2618388	2619677
JW2483(upp)	Complementary strand	2619763	2620389
JW2484(purM)		2620714	2621751
JW2485(purN)		2621751	2622389
JW2486(ppk)		2622561	2624627
JW2487(ppx)		2624632	2626173
JW2488(427#1)	Complementary strand	2626212	2628455
JW2489(427#2)		2629312	2629827
JW2490(427#3)		2629843	2630382
JW2491(guaA)	Complementary strand	2630475	2632052
JW2492(guaB)	Complementary strand	2632121	2633656
JW2493(xseA)		2633749	2635119
JW2494(yfgJ)	Complementary strand	2635116	2635367
JW2495(yfgK)	Complementary strand	2635401	2636912
JW2496(yfgL)	Complementary strand	2636991	2638169
JW2497(yfgM)	Complementary strand	2638180	2638800
JW2498(hisS)	Complementary strand	2638818	2640092
JW2499(gcpE)	Complementary strand	2640203	2641321
JW2500(yfgA)	Complementary strand	2641348	2642361
JW2501(yfgB)	Complementary strand	2642646	2643800
JW2502(ndk)	Complementary strand	2643950	2644381
JW2503(pbpC)	Complementary strand	2644530	2646842
JW2504(429#5)	Complementary strand	2646843	2651804
JW2505(sseA)		2652011	2652856
JW2506(sseB)	Complementary strand	2653674	2654315
JW2507(pepB)	Complementary strand	2654592	2655875
JW2508(yfhJ)	Complementary strand	2656053	2656253
JW2509(fdx)	Complementary strand	2656265	2656600
JW2510(hscA)	Complementary strand	2656602	2658452
JW2511(hscB)	Complementary strand	2658469	2658984
JW2512(yfhF)	Complementary strand	2659080	2659403
JW2513(iscU)	Complementary strand	2659420	2659806
JW2514(iscS)	Complementary strand	2659834	2661048
JW2515(iscR)	Complementary strand	2661160	2661648
JW2516(yfhQ)	Complementary strand	2662100	2662840
JW2517(suhB)		2662959	2663762
JW2518(431#2)		2663880	2664761
JW2519(csiE)		2664952	2666343
JW2520(yfhS)	Complementary strand	2666225	2667364
JW2521(yfhT)	Complementary strand	2667524	2668414
JW2522(dgxA)		2668550	2669911
JW2523(dgxB)		2669908	2670426
JW2524(hcaC)		2670426	2670746
JW2525(hcaB)		2670743	2671555
JW2526(hcaD)		2671565	2672767
JW2527(yphA)		2672792	2673286
JW2528(yphB)	Complementary strand	2673334	2674206
JW2529(yphC)	Complementary strand	2674218	2675312
JW2530(yphD)	Complementary strand	2675345	2676343
JW2531(yphE)	Complementary strand	2676368	2677879
JW2532(yphF)	Complementary strand	2677902	2678885
JW2533(yphG)	Complementary strand	2678982	2682356
JW2534(yphH)		2682375	2683574
JW2535(glyA)	Complementary strand	2683772	2685025
JW2536(hmpA)		2685353	2686543
JW2537(glnB)	Complementary strand	2686588	2686926
JW2538(yfhA)	Complementary strand	2686987	2688321
JW2539(yfhG)	Complementary strand	2688311	2689024
JW2540(yfhK)	Complementary strand	2689189	2690571
JW2541(purL)	Complementary strand	2691174	2695061
JW2542(yfhD)		2695457	2696875
JW2543(yfhC)	Complementary strand	2696872	2697408
JW2544(yfhB)	Complementary strand	2697433	2698005
JW2545(yfhH)		2698277	2699125
JW2546(yfhL)		2699181	2699441
JW2547(acpS)	Complementary strand	2700136	2700516
JW2548(pdxJ)	Complementary strand	2700516	2701247
JW2549(recO)	Complementary strand	2701259	2701987
JW2550(era)	Complementary strand	2701999	2702904
JW2551(rnc)	Complementary strand	2702901	2703581
JW2552(lepB)	Complementary strand	2703853	2704827
JW2553(lepA)	Complementary strand	2704843	2706642
JW2554(rseC)	Complementary strand	2706840	2707319
JW2555(rseB)	Complementary strand	2707316	2708272
JW2556(rseA)	Complementary strand	2708272	2708922
JW2557(rpoE)	Complementary strand	2708955	2709530
JW2558(nadB)		2709938	2711560
JW2559(yfiC)	Complementary strand	2711545	2712282
JW2560(srmB)		2712414	2713748
JW2561(yfiE)	Complementary strand	2713957	2714838
JW2562(yfiK)		2714941	2715528
JW2563(yfiD)	Complementary strand	2715584	2715967
JW2564(ung)		2716272	2716961
JW2565(yfiF)	Complementary strand	2717009	2718046
JW2566(trx2)		2718253	2718672
JW2567(yfiP)		2718717	2719439
JW2568(yfiQ)		2719471	2722131
JW2569(pssA)		2722245	2723600
JW2570(yfiM)		2723697	2723969
JW2571(kgtP)	Complementary strand	2723966	2725264
JW2572(437#4)		2725443	2725505
JW2573(clpB)	Complementary strand	2731118	2733691
JW2574(437#6.1)		2733777	2733875
JW2575(yfiH)	Complementary strand	2733821	2734552
JW2576(rluD)	Complementary strand	2734549	2735529
JW2577(yfiO)		2735664	2736401
JW2578(yfiA)		2736672	2737013
JW2579(pheL)		2737117	2737164
JW2580(pheA)		2737263	2738423
JW2581(tyrA)	Complementary strand	2738466	2739587
JW2582(aroF)	Complementary strand	2739598	2740668
JW2583(yfiL)		2740839	2741243
JW2584(yfiR)		2741393	2741911
JW2585(yfiN)		2741901	2743127
JW2586(yfiB)		2743143	2743625
JW2587(rplS)	Complementary strand	2743701	2744048
JW2588(trmD)	Complementary strand	2744090	2744857
JW2589(rimM)	Complementary strand	2744888	2745445
JW2590(rpsP)	Complementary strand	2745455	2745703
JW2591(ffh)	Complementary strand	2745952	2747058
JW2592(439#12)		2747405	2748271
JW2593(yfjD)		2748337	2749578
JW2594(grpE)	Complementary strand	2749633	2750226
JW2595(439#15)	Complementary strand	2750223	2750396
JW2596(yfjB)		2750349	2751227
JW2597(recN)		2751313	2752974
JW2598(smpA)		2753123	2753464
JW2599(yfjF)	Complementary strand	2753526	2753816
JW2600(yfjG)	Complementary strand	2753806	2754282
JW2601(smpB)		2754414	2754896
JW2602(intA)		2755677	2756918
JW2603(yfjH)	Complementary strand	2757162	2758118
JW2604(alpA)		2758162	2758374
JW2605(yfjI)		2758503	2759912
JW2606(440#5.1)	Complementary strand	2759865	2760071
JW2607(yfjJ)		2760065	2760691
JW2608(yfjK)	Complementary strand	2760869	2763058
JW2609(yfjL)	Complementary strand	2763055	2764671
JW2610(yfjM)	Complementary strand	2765031	2765294
JW2611(yfjN)		2765436	2766509
JW2612(yfjO)		2766553	2766873
JW2613(yfjP)		2767222	2768091
JW2614(yfjQ)		2768183	2769004
JW2615(yfjR)		2769221	2769922
JW2616(ypjK)		2769807	2770199
JW2617(yfjS)		2769950	2770642
JW2618(yfjT)		2770666	2771133
JW2619(yfjU)	Complementary strand	2771358	2771672
JW2620(yfjV)	Complementary strand	2771685	2772278
JW2621(yfjV)	Complementary strand	2772354	2772554
JW2622(442#2.2)	Complementary strand	2772494	2772610
JW2623(yfjW)		2772836	2774539
JW2623.5(442#4)		2775237	2775440
JW2624(yfjX)		2775437	2775895
JW2625(yfjY)		2775904	2776386
JW2626(yfjZ)		2776633	2776950
JW2627(ypjF)		2776971	2777300
JW2628(ypjA)	Complementary strand	2777664	2782373
JW2629(443#1.1)	Complementary strand	2783156	2783947
JW2630(443#1.2)	Complementary strand	2784047	2784445
JW2631(443#2)		2786266	2786952
JW2632(443#2.1)		2787343	2787756
JW2633(443#2.2)		2787895	2788167
JW2634(443#3)		2788398	2789480
JW2635(ygaF)		2789500	2790768
JW2636(gabD)		2790791	2792239
JW2637(gabT)		2792253	2793533
JW2638(gabP)		2793771	2795171
JW2639(ygaE)		2795192	2795854
JW2640(yzzM)	Complementary strand	2795855	2796304
JW2641(yqaE)	Complementary strand	2796388	2796546
JW2642(445#2)		2796729	2797028
JW2643(ygaP)		2797038	2797562
JW2644(stpA)	Complementary strand	2797609	2798013
JW2645(445#5)		2798682	2799131
JW2646(ygaC)	Complementary strand	2799168	2799512
JW2647(ygaM)		2799652	2799993
JW2648(nrdH)		2800241	2800486
JW2649(nrdI)		2800483	2800893
JW2650(nrdE)		2800866	2803010
JW2651(nrdF)		2803020	2803979
JW2652(proV)		2804333	2805535
JW2653(proW)		2805528	2806592
JW2654(proX)		2806650	2807642
JW2655(445#15)		2807834	2808100
JW2656(445#17)		2808094	2809011
JW2657(ygaZ)		2809135	2809872
JW2658(ygaH)		2809862	2810197
JW2659(emrR)		2810288	2810818
JW2660(emrA)		2810945	2812117
JW2661(emrB)		2812134	2813672
JW2662(ygaG)	Complementary strand	2813736	2814251
JW2663(gshA)	Complementary strand	2814401	2815957
JW2664(yqaA)	Complementary strand	2816030	2816458
JW2665(yqaB)	Complementary strand	2816455	2817021
JW2666(csrA)	Complementary strand	2818479	2818664
JW2667(alaS)	Complementary strand	2818899	2821529
JW2668(oraA)	Complementary strand	2821657	2822157
JW2669(recA)	Complementary strand	2822226	2823287
JW2670(ygaD)	Complementary strand	2823367	2823864
JW2671(mltB)	Complementary strand	2824009	2825094
JW2672(srlA)		2825350	2826870
JW2673(srlB)		2826881	2827252
JW2674(srlD)		2827256	2828035
JW2675(gutM)		2828141	2828500
JW2676(srlR)		2828567	2829340
JW2677(gutQ)		2829372	2830298
JW2678(ygaA)	Complementary strand	2830295	2830807
JW2679(ygaA)	Complementary strand	2830827	2831885
JW2680(448#2)		2831997	2833436
JW2681(ygbD)		2833433	2834566
JW2682(hypF)	Complementary strand	2834694	2836946
JW2683(hydN)	Complementary strand	2837099	2837626
JW2684(ascG)	Complementary strand	2837775	2838785
JW2685(ascF)		2839045	2840502
JW2686(ascB)		2840511	2841935
JW2687(hycI)	Complementary strand	2842094	2842564
JW2688(hycH)	Complementary strand	2842557	2842967
JW2689(hycG)	Complementary strand	2842964	2843731
JW2690(hycF)	Complementary strand	2843731	2844273
JW2691(hycE)	Complementary strand	2844283	2845992
JW2692(hycD)	Complementary strand	2846010	2846933
JW2693(hycC)	Complementary strand	2846936	2848762
JW2694(hycB)	Complementary strand	2848759	2849370
JW2695(hycA)	Complementary strand	2849495	2849956
JW2696(hypA)		2850168	2850518
JW2697(hypB)		2850522	2851394
JW2698(hypC)		2851385	2851657
JW2699(hypD)		2851657	2852778
JW2700(hypE)		2852817	2853785
JW2701(fhlA)		2853859	2855937
JW2702(ygbA)	Complementary strand	2855974	2856327
JW2703(mutS)		2856614	2859175
JW2704(prpB)		2859281	2859937
JW2705(ygbI)	Complementary strand	2859988	2860785
JW2706(ygbJ)		2860952	2861860
JW2707(ygbK)		2861857	2863023
JW2708(ygbL)		2863115	2863753
JW2709(ygbM)		2863758	2864534
JW2710(ygbN)		2864623	2865987
JW2711(rpoS)	Complementary strand	2866081	2867073
JW2712(nlpD)	Complementary strand	2867136	2868275
JW2713(pcm)	Complementary strand	2868415	2869041
JW2714(surE)	Complementary strand	2869035	2869796
JW2715(ygbO)	Complementary strand	2869777	2870826
JW2716(ygbB)	Complementary strand	2870823	2871302
JW2717(ygbP)	Complementary strand	2871302	2872012
JW2718(ygbQ)	Complementary strand	2872031	2872342
JW2719(ygbE)	Complementary strand	2872534	2872857
JW2720(cysC)	Complementary strand	2872907	2873512
JW2721(cysN)	Complementary strand	2873512	2874939
JW2722(cysD)	Complementary strand	2874941	2875849
JW2723(iap)		2876101	2877138
JW2724(ygbF)	Complementary strand	2878089	2878439
JW2725(452#12)	Complementary strand	2878375	2879292
JW2726(ygcH)	Complementary strand	2879308	2879907
JW2727(452#14)	Complementary strand	2879894	2880568
JW2728(ygcJ)	Complementary strand	2880571	2881662
JW2729(ygcK)	Complementary strand	2881675	2882157
JW2730(453#2)	Complementary strand	2882150	2883658
JW2731(ygcB)	Complementary strand	2884073	2886739
JW2732(cysH)	Complementary strand	2887098	2887832
JW2733(cysI)	Complementary strand	2887907	2889619
JW2734(cysJ)	Complementary strand	2889619	2891418
JW2735(ptpS)		2891734	2892099
JW2736(ygcN)		2892177	2893448
JW2737(ygcO)		2893439	2893699
JW2738(ygcP)		2893716	2894291
JW2739(ygcQ)	Complementary strand	2894439	2895332
JW2740(ygcR)	Complementary strand	2895366	2896076
JW2741(ygcS)	Complementary strand	2896054	2897463
JW2742(454#9)	Complementary strand	2897485	2898279
JW2743(ygcU)	Complementary strand	2898282	2898647
JW2744(ygcU)	Complementary strand	2898656	2898937
JW2745(ygcW)	Complementary strand	2899007	2899867
JW2746(yqcE)		2900111	2901388
JW2747(ygcE)		2901769	2902893
JW2748(ygcF)	Complementary strand	2904267	2904938
JW2749(ygcG)		2905162	2906103
JW2750(eno)	Complementary strand	2906163	2907461
JW2751(pyrG)	Complementary strand	2907549	2909186
JW2752(mazG)	Complementary strand	2909414	2910205
JW2753(chpA)	Complementary strand	2910276	2910611
JW2754(chpR)	Complementary strand	2910611	2910859
JW2755(relA)	Complementary strand	2910937	2913171
JW2756(ygcA)	Complementary strand	2913219	2914520
JW2757(barA)		2914577	2917333
JW2758(ygcX)	Complementary strand	2917565	2918905
JW2759(gud2)	Complementary strand	2918926	2920266
JW2760(gudT)	Complementary strand	2920268	2921620
JW2761(mioC)	Complementary strand	2922055	2922504
JW2762(yqcB)	Complementary strand	2922522	2923304
JW2763(yqcC)	Complementary strand	2923304	2923633
JW2764(syd)	Complementary strand	2924255	2924800
JW2765(yqcD)		2924868	2925716
JW2766(ygdH)		2925828	2927192
JW2767(sdaC)		2927749	2929038
JW2768(sdaB)		2929096	2930463
JW2769(exo)		2930575	2931330
JW2770(fucO)	Complementary strand	2931385	2932536
JW2771(fucA)	Complementary strand	2932561	2933208
JW2772(fucP)		2933755	2935071
JW2773(fucI)		2935104	2936879
JW2774(fucK)		2936958	2938406
JW2775(fucU)		2938408	2938830
JW2776(fucR)		2938888	2939619
JW2777(ygdE)	Complementary strand	2939663	2940763
JW2778(ygdD)	Complementary strand	2940756	2941151
JW2779(gcvA)	Complementary strand	2941170	2942087
JW2780(ygdI)	Complementary strand	2942438	2942668
JW2781(nifS)		2942857	2944062
JW2782(ygdK)		2944062	2944505
JW2783(ygdL)	Complementary strand	2944556	2945362
JW2784(mltA)	Complementary strand	2945601	2946698
JW2785(amiC)	Complementary strand	2947277	2948620
JW2786(argA)		2948762	2950093
JW2787(recD)	Complementary strand	2950155	2951981
JW2788(recB)	Complementary strand	2951981	2955523
JW2789(ptr)	Complementary strand	2955516	2958404
JW2790(recC)	Complementary strand	2958580	2961948
JW2791(ppdC)	Complementary strand	2961961	2962284
JW2792(ygdB)	Complementary strand	2962269	2962634
JW2793(ppdB)	Complementary strand	2962673	2963476
JW2794(ppdA)	Complementary strand	2963227	2963697
JW2795(thyA)	Complementary strand	2963881	2964675
JW2796(lgt)	Complementary strand	2964682	2965557
JW2797(ptsP)	Complementary strand	2965708	2967954
JW2798(ygdP)	Complementary strand	2967967	2968497
JW2799(mutH)		2969182	2969871
JW2800(ygdQ)		2969940	2970653
JW2801(ygdR)		2970791	2971009
JW2802(tas)		2971117	2972157
JW2803(ygeD)	Complementary strand	2972189	2973382
JW2804(aas)	Complementary strand	2973375	2975534
JW2805(galR)		2976119	2977150
JW2806(lysA)	Complementary strand	2977157	2978419
JW2807(lysR)		2978541	2979476
JW2808(ygeA)	Complementary strand	2979463	2980155
JW2809(araE)	Complementary strand	2980284	2981702
JW2810(kduD)	Complementary strand	2982017	2982778
JW2811(kduI)	Complementary strand	2982808	2983644
JW2812(yqeF)	Complementary strand	2983931	2985115
JW2813(sdaC)		2985367	2986596
JW2814(yqeH)		2987023	2987688
JW2815(yqeI)		2988022	2988831
JW2816(yqeJ)		2988992	2989306
JW2817(yqeK)	Complementary strand	2989455	2989880
JW2818(ygeF)		2990074	2990520
JW2819(ygeG)		2990788	2991279
JW2820(ygeH)		2991614	2992990
JW2821(ygeI)		2993128	2993376
JW2822(x)		2993520	2993936
JW2823(ygeK)	Complementary strand	2993981	2994427
JW2824(464#6)	Complementary strand	2994458	2994613
JW2825(464#7)	Complementary strand	2995269	2995523
JW2826(yi22)	Complementary strand	2995893	2996798
JW2827(yi24)		2996322	2996753
JW2828(yi23)	Complementary strand	2996432	2996725
JW2829(yi2A)	Complementary strand	2996756	2997166
JW2830(465#0.1)		2997078	2997245
JW2831(ygeP)	Complementary strand	2997210	2997509
JW2832(465#1)	Complementary strand	2997555	2998391
JW2833(nlpD)	Complementary strand	2998657	2999412
JW2834(yagR)		2999827	3002124
JW2835(ygeT)		3002135	3003013
JW2836(465#5)		3003010	3003489
JW2837(ygeV)	Complementary strand	3003529	3005307
JW2838(ygeW)		3005854	3006945
JW2839(dpaL)		3007029	3008225
JW2840(ygeY)		3008283	3009494
JW2841(ygeZ)		3009547	3010932
JW2842(arcL)		3010980	3011912
JW2843(yqeB)	Complementary strand	3012133	3013758
JW2844(466#6)	Complementary strand	3013806	3014477
JW2845(ygfJ)		3014679	3015257
JW2846(gltD)		3015579	3018677
JW2847(467#1)		3018680	3020008
JW2848(ygfM)		3020059	3020838
JW2849(yagR)		3020835	3023705
JW2850(ygfO)		3023870	3025270
JW2851(ygfP)		3025165	3026604
JW2852(ygfQ)		3026640	3027209
JW2853(ygfR)		3027176	3028006
JW2854(ygfS)	Complementary strand	3028042	3028533
JW2855(ygfT)	Complementary strand	3028530	3030464
JW2856(pbuX)		3030816	3032333
JW2857(idi)		3032583	3033131
JW2858(lysS)	Complementary strand	3033175	3034692
JW2859(prfB)	Complementary strand	3034702	3035367
JW2860(recJ)	Complementary strand	3035891	3037624
JW2861(dsbC)	Complementary strand	3037630	3038340
JW2862(xerD)	Complementary strand	3038365	3039261
JW2863(fldB)		3039373	3039894
JW2864(ygfX)	Complementary strand	3039934	3040341
JW2865(ygfY)	Complementary strand	3040322	3040588
JW2866(up14)		3040831	3041811
JW2867(yqfA)	Complementary strand	3042007	3042666
JW2868(yqfB)	Complementary strand	3042830	3043141
JW2869(bglA)		3043180	3044619
JW2870(ygfF)	Complementary strand	3044676	3045419
JW2871(gcvP)	Complementary strand	3045686	3048559
JW2872(gcvH)	Complementary strand	3048678	3049067
JW2873(gcvT)	Complementary strand	3049091	3050185
JW2874(visC)	Complementary strand	3050633	3051835
JW2875(ubiH)	Complementary strand	3051858	3053036
JW2876(pepP)	Complementary strand	3053033	3054358
JW2877(ygfB)	Complementary strand	3054384	3054968
JW2878(ygfE)		3055130	3055459
JW2879(ygfA)		3055759	3056307
JW2880(serA)	Complementary strand	3056696	3057928
JW2881(rpiA)	Complementary strand	3058184	3058879
JW2882(yqfE)	Complementary strand	3058899	3059129
JW2883(iciA)		3059271	3060164
JW2884(sbm)		3060368	3062512
JW2885(argK)		3062505	3063500
JW2886(ygfG)		3063511	3064296
JW2887(ygfH)		3064320	3065798
JW2888(ygfI)	Complementary strand	3065795	3066706
JW2889(yggE)	Complementary strand	3066858	3067598
JW2890(yggA)	Complementary strand	3067691	3068326
JW2891(yggB)	Complementary strand	3068465	3069325
JW2892(fba)	Complementary strand	3069683	3070762
JW2893(pgk)	Complementary strand	3070977	3072140
JW2894(epd)	Complementary strand	3072190	3073209
JW2895(yggC)	Complementary strand	3073494	3074207
JW2896(yggD)	Complementary strand	3074204	3074713
JW2897(yggF)	Complementary strand	3074735	3075700
JW2898(yggP)	Complementary strand	3075697	3076686
JW2899(yggP)	Complementary strand	3076617	3076973
JW2900(cmtA)	Complementary strand	3076988	3078376
JW2901(cmtB)	Complementary strand	3078404	3078847
JW2902(tktA)	Complementary strand	3079161	3081155
JW2903(yggG)		3081435	3082193
JW2904(speB)	Complementary strand	3082399	3083319
JW2905(speA)	Complementary strand	3083457	3085433
JW2906(yqgB)	Complementary strand	3085442	3085588
JW2907(yqgC)		3085709	3085924
JW2908(yqgD)	Complementary strand	3085921	3086172
JW2909(metK)		3086228	3087382
JW2910(galP)		3087805	3089199
JW2911(sprT)		3089276	3089773
JW2912(endA)		3089868	3090575
JW2913(yggJ)		3090655	3091386
JW2914(gshB)		3091399	3092349
JW2915(yqgE)		3092458	3093021
JW2916(yqgF)		3093021	3093437
JW2917(yggR)	Complementary strand	3093621	3094601
JW2918(yggS)		3094619	3095323
JW2919(yggT)		3095341	3095907
JW2920(yggU)		3095892	3096194
JW2921(yggV)		3096202	3096795
JW2922(yggW)		3096788	3097924
JW2923(yggM)	Complementary strand	3098079	3099086
JW2924(ansB)	Complementary strand	3099203	3100249
JW2925(yggN)	Complementary strand	3100425	3101144
JW2926(yggL)	Complementary strand	3101327	3101653
JW2927(yggH)	Complementary strand	3101653	3102372
JW2928(mutY)		3102533	3103585
JW2929(yggX)		3103613	3103888
JW2930(mltC)		3103950	3104489
JW2931(mltC)		3104536	3105033
JW2932(nupG)		3105235	3106491
JW2933(speC)	Complementary strand	3106541	3108736
JW2934(yqgA)		3109073	3109780
JW2935(epsM)	Complementary strand	3110110	3110646
JW2936(exeL)	Complementary strand	3110648	3111490
JW2937(exeC)	Complementary strand	3111574	3112569
JW2938(477#5)	Complementary strand	3112587	3112997
JW2939(477#6)	Complementary strand	3113063	3113872
JW2940(477#7)	Complementary strand	3114070	3116616
JW2941(478#1)	Complementary strand	3116604	3118631
JW2942(yghK)	Complementary strand	3119116	3120798
JW2943(glcB)	Complementary strand	3121153	3123324
JW2944(glcG)	Complementary strand	3123346	3123750
JW2945(glcF)	Complementary strand	3123755	3126040
JW2946(glcD)	Complementary strand	3126040	3127539
JW2947(glcC)		3127790	3128554
JW2948(502#6)	Complementary strand	3128561	3129733
JW2949(is5B)		3129696	3130712
JW2950(yi51)	Complementary strand	3130008	3130376
JW2951(yghQ)	Complementary strand	3130859	3131788
JW2952(yghR)	Complementary strand	3131972	3132730
JW2953(yghS)	Complementary strand	3132762	3133460
JW2954(yghT)		3133649	3134341
JW2955(pitB)	Complementary strand	3134390	3135889
JW2956(gsp)	Complementary strand	3136181	3138040
JW2957(yghU)		3138197	3139111
JW2958(hybG)	Complementary strand	3139234	3139482
JW2959(hybF)	Complementary strand	3139519	3139836
JW2960(hybE)	Complementary strand	3139829	3140317
JW2961(hybD)	Complementary strand	3140310	3140804
JW2962(hybC)	Complementary strand	3140804	3142507
JW2963(hybB)	Complementary strand	3142504	3143679
JW2964(hybA)	Complementary strand	3143669	3144655
JW2965(hoxK)	Complementary strand	3144658	3145776
JW2966(yghW)	Complementary strand	3145965	3146252
JW2967(504#11)	Complementary strand	3146371	3146781
JW2968(504#12)	Complementary strand	3146781	3147206
JW2969(504#13)	Complementary strand	3147112	3147300
JW2970(yajO)		3147412	3148452
JW2971(yqhA)	Complementary strand	3148492	3148986
JW2972(yghA)		3149177	3150061
JW2973(exbD)	Complementary strand	3150333	3150758
JW2974(exbB)	Complementary strand	3150765	3151499
JW2975(metC)		3151751	3152938
JW2976(yghB)		3153078	3153737
JW2977(exsA)	Complementary strand	3153776	3154903
JW2978(yqhD)		3154869	3156032
JW2979(yghE)		3156370	3156963
JW2980(yqhG)		3157163	3157456
JW2981(yqhG)		3157414	3158091
JW2982(yqhH)		3158142	3158399
JW2983(ygiQ)	Complementary strand	3158442	3159683
JW2984(ygiR)	Complementary strand	3159683	3160660
JW2985(sufI)	Complementary strand	3160771	3162183
JW2986(plsC)	Complementary strand	3162258	3162995
JW2987(parC)	Complementary strand	3163229	3165487
JW2988(ygiS)	Complementary strand	3165625	3167232
JW2989(ygiT)	Complementary strand	3167365	3167760
JW2990(ygiU)	Complementary strand	3167762	3168058
JW2991(506#5)	Complementary strand	3168263	3168745
JW2992(ygiW)	Complementary strand	3168798	3169190
JW2993(ygiX)		3169342	3170001
JW2994(ygiY)		3169998	3171347
JW2995(ygiZ)	Complementary strand	3171393	3171725
JW2996(mdaB)		3172045	3172626
JW2997(ygiN)		3172657	3172971
JW2998(parE)	Complementary strand	3173019	3174911
JW2999(yqiA)	Complementary strand	3174940	3175521
JW3000(icc)	Complementary strand	3175521	3176348
JW3001(yqiB)	Complementary strand	3176373	3176795
JW3002(yqiE)	Complementary strand	3176796	3177425
JW3003(tolC)		3177624	3179111
JW3004(507#7)		3179111	3179371
JW3005(ygiB)		3179226	3179930
JW3006(ygiC)		3179936	3181096
JW3007(ygiD)	Complementary strand	3181134	3181949
JW3008(ygiE)		3182065	3182838
JW3009(ribB)	Complementary strand	3183328	3183981
JW3010(yqiC)		3184295	3184645
JW3011(ygiL)		3184929	3185480
JW3012(yi2A)		3185657	3186067
JW3013(yi22)		3186025	3186930
JW3014(yi24)	Complementary strand	3186070	3186501
JW3015(yi23)		3186098	3186391
JW3016(yqiG)		3186915	3187859
JW3017(yqiG)		3187684	3189381
JW3018(yqiH)		3189388	3190146
JW3019(yqiI)		3190148	3190855
JW3020(yqiI)		3190791	3191213
JW3021(glgS)	Complementary strand	3191256	3191456
JW3022(508#6)		3191725	3192354
JW3023(508#7)		3192381	3194042
JW3024(rfaE)	Complementary strand	3194837	3196270
JW3025(glnE)	Complementary strand	3196318	3199158
JW3026(ygiF)	Complementary strand	3199181	3200482
JW3027(ygiM)		3200724	3201344
JW3028(cca)		3201408	3202646
JW3029(bacA)	Complementary strand	3202827	3203648
JW3030(folB)	Complementary strand	3203738	3204106
JW3031(ygiH)		3204211	3204828
JW3032(ygiP)	Complementary strand	3204841	3205773
JW3033(ttdA)		3205980	3206891
JW3034(ttdB)		3206888	3207493
JW3035(ygjE)		3207541	3209004
JW3036(ygjD)	Complementary strand	3209047	3210060
JW3037(rpsU)		3210298	3210513
JW3038(dnaG)		3210624	3212369
JW3039(rpoD)		3212564	3214405
JW3040(ygjF)	Complementary strand	3214484	3214990
JW3041(yqjH)	Complementary strand	3215244	3216008
JW3042(yqjI)		3216296	3216919
JW3043(aer)	Complementary strand	3217073	3218593
JW3044(ygjG)		3219101	3220390
JW3045(ygjH)	Complementary strand	3220432	3220764
JW3046(ebgR)		3220983	3221966
JW3047(ebgA)		3222150	3225242
JW3048(ebgC)		3225239	3225688
JW3049(ygjI)		3225751	3227184
JW3050(ygjJ)		3227318	3228388
JW3051(ygjK)		3228405	3230756
JW3052(fadH)		3231182	3233200
JW3053(ygiM)	Complementary strand	3233245	3233661
JW3054(ygjN)	Complementary strand	3233658	3233972
JW3055(ygjO)	Complementary strand	3234256	3235422
JW3056(ygjP)		3235441	3235980
JW3057(ygjQ)		3236057	3236749
JW3058(ygjR)		3236810	3237814
JW3059(ygjT)		3238098	3239063
JW3060(ygjU)		3239460	3240704
JW3061(ygjV)	Complementary strand	3240709	3241260
JW3062(uxaA)	Complementary strand	3241343	3242830
JW3063(uxaC)	Complementary strand	3242845	3244257
JW3064(exuT)		3244620	3246038
JW3065(exuR)		3246168	3246944
JW3066(yqjA)		3247289	3247951
JW3067(yqjB)		3247955	3248338
JW3068(yqjC)		3248470	3248853
JW3069(yqjD)		3248891	3249196
JW3070(yqjE)		3249199	3249603
JW3071(yqjK)		3249593	3249892
JW3072(yqjF)		3249988	3250470
JW3073(yqjG)		3250540	3251526
JW3074(yhaH)		3251820	3252185
JW3075(yhaI)		3252427	3252783
JW3076(yhaJ)	Complementary strand	3252834	3253730
JW3077(yhaK)		3253835	3254536
JW3078(yhaL)		3254553	3254723
JW3079(yhaM)	Complementary strand	3254857	3255423
JW3080(yhaN)	Complementary strand	3255605	3256168
JW3081(yhaO)	Complementary strand	3256196	3257473
JW3082(yhaP)	Complementary strand	3257804	3258631
JW3083(yhaQ)	Complementary strand	3258751	3259173
JW3084(yhaR)	Complementary strand	3259239	3259691
JW3085(tdcE)	Complementary strand	3259695	3261935
JW3086(tdcD)	Complementary strand	3261969	3263177
JW3087(tdcC)	Complementary strand	3263203	3264534
JW3088(tdcB)	Complementary strand	3264556	3265545
JW3089(tdcA)	Complementary strand	3265644	3266582
JW3090(tdcR)		3266816	3267115
JW3091(yhaB)		3267371	3267910
JW3092(yhaC)		3267932	3269119
JW3093(yhaD)	Complementary strand	3270142	3271368
JW3094(yhaE)	Complementary strand	3271384	3272283
JW3095(yhaF)	Complementary strand	3272304	3273074
JW3096(yhaU)	Complementary strand	3273090	3274424
JW3097(yhaG)		3274799	3276310
JW3098(sohA)		3276519	3276854
JW3099(yhaV)		3276854	3277318
JW3100(agaR)	Complementary strand	3277373	3278182
JW3101(agaZ)		3278431	3279711
JW3102(agaV)		3279734	3280207
JW3103(agaW)		3280218	3280619
JW3104(agaA)		3280639	3281142
JW3105(agaS)		3281493	3282647
JW3106(agaY)		3282660	3283520
JW3107(agaB)		3283687	3284163
JW3108(agaC)		3284202	3285005
JW3109(agaD)		3284995	3285786
JW3110(agaI)		3285787	3286542
JW3111(yraH)		3286943	3287527
JW3112(yraI)		3287607	3288302
JW3113(yraJ)		3288331	3290847
JW3114(yraK)		3290858	3291949
JW3115(yraL)	Complementary strand	3291992	3292852
JW3116(yraM)		3292917	3294953
JW3117(yraN)		3294911	3295306
JW3118(yraO)		3295326	3295916
JW3119(yraP)		3295926	3296501
JW3120(yraQ)	Complementary strand	3296615	3297655
JW3121(yraR)	Complementary strand	3297728	3298408
JW3122(yhbO)		3298449	3299009
JW3123(yhbP)	Complementary strand	3298989	3299432
JW3124(yhbQ)		3299483	3299785
JW3125(yhbS)	Complementary strand	3299772	3300275
JW3126(yhbT)	Complementary strand	3300269	3300793
JW3127(yhbU)		3301002	3301997
JW3128(yhbV)		3301988	3302884
JW3129(yhbW)		3302965	3303972
JW3130(mtr)	Complementary strand	3304090	3305334
JW3131(deaD)	Complementary strand	3305488	3307428
JW3132(nlpI)	Complementary strand	3307557	3308441
JW3133(pnp)	Complementary strand	3308550	3310685
JW3134(rpsO)	Complementary strand	3310932	3311201
JW3135(truB)	Complementary strand	3311350	3312294
JW3136(rbfA)	Complementary strand	3312294	3312695
JW3137(infB)	Complementary strand	3312859	3315531
JW3138(nusA)	Complementary strand	3315556	3317043
JW3139(yhbC)	Complementary strand	3317071	3317493
JW3140(argG)		3318154	3319497
JW3141(yhbX)	Complementary strand	3319505	3321148
JW3142(secG)	Complementary strand	3321690	3322022
JW3143(mrsA)	Complementary strand	3322250	3323587
JW3144(folP)	Complementary strand	3323580	3324428
JW3145(ftsH)	Complementary strand	3324518	3326452
JW3146(ftsJ)	Complementary strand	3326552	3327181
JW3147(yhbY)		3327307	3327600
JW3148(greA)	Complementary strand	3327756	3328232
JW3149(dacB)		3328480	3329913
JW3150(yhbZ)	Complementary strand	3330099	3331271
JW3151(yhbE)	Complementary strand	3331287	3332252
JW3152(rpmA)	Complementary strand	3332379	3332636
JW3153(rplU)	Complementary strand	3332657	3332968
JW3154(ispB)		3333227	3334198
JW3155(nlp)		3334426	3334704
JW3156(murA)	Complementary strand	3334752	3336011
JW3157(yrbA)	Complementary strand	3336066	3336320
JW3158(yrbB)	Complementary strand	3336480	3336869
JW3159(yrbC)	Complementary strand	3336773	3337408
JW3160(yrbD)	Complementary strand	3337427	3337978
JW3161(yrbE)	Complementary strand	3337983	3338765
JW3162(yrbF)	Complementary strand	3338773	3339582
JW3163(yrbG)		3339792	3340769
JW3164(yrbH)		3340783	3341769
JW3165(yrbI)		3341790	3342356
JW3166(yrbK)		3342353	3342928
JW3167(yhbN)		3342897	3343454
JW3168(yhbG)		3343461	3344186
JW3169(rpoN)		3344234	3345667
JW3170(yhbH)		3345690	3345977
JW3171(ptsN)		3346095	3346586
JW3172(yhbJ)		3346632	3347486
JW3173(ptsO)		3347483	3347755
JW3174(yrbL)		3347969	3348601
JW3175(mtgA)	Complementary strand	3348598	3349326
JW3176(yhbL)	Complementary strand	3349323	3349976
JW3177(arcB)	Complementary strand	3350206	3352536
JW3178(yhcC)	Complementary strand	3352632	3353561
JW3179(gltB)		3354143	3358696
JW3180(gltD)		3358709	3360127
JW3181(gltF)		3360687	3361451
JW3182(yhcA)		3361623	3362297
JW3183(yhcD)		3362318	3364699
JW3184(yhcE)		3364696	3365175
JW3185(is5B)	Complementary strand	3365213	3366229
JW3186(yi51)		3365549	3365917
JW3187(yhcE)		3366192	3366440
JW3188(yhcF)		3366437	3367153
JW3189(yhcG)		3367338	3368465
JW3190(yhcH)	Complementary strand	3368525	3368989
JW3191(nanK)	Complementary strand	3368986	3369894
JW3192(nanE)	Complementary strand	3369858	3370547
JW3193(nanT)	Complementary strand	3370595	3372085
JW3194(npl)	Complementary strand	3372194	3373087
JW3195(nanR)	Complementary strand	3373209	3374000
JW3196(dcuD)		3374380	3375747
JW3197(sspB)	Complementary strand	3375790	3376287
JW3198(sspA)	Complementary strand	3376293	3376931
JW3199(rpsI)	Complementary strand	3377326	3377718
JW3200(rplM)	Complementary strand	3377734	3378162
JW3201(yhcM)	Complementary strand	3378381	3379508
JW3202(yhcB)		3379696	3380100
JW3203(hhoA)		3380254	3381621
JW3204(hhoB)		3381711	3382778
JW3205(mdh)	Complementary strand	3382841	3383779
JW3206(argR)		3384214	3384684
JW3207(yhcN)		3384998	3385312
JW3208(yhcO)	Complementary strand	3385368	3385640
JW3209(yhcP)	Complementary strand	3385732	3387699
JW3210(yhcQ)	Complementary strand	3387705	3388637
JW3211(yhcR)	Complementary strand	3388645	3388917
JW3212(yhcS)		3389031	3389960
JW3213(tldD)	Complementary strand	3390094	3391539
JW3214(yhdP)	Complementary strand	3391970	3394930
JW3215(yhdR)	Complementary strand	3394923	3395771
JW3216(rng)	Complementary strand	3395839	3397308
JW3217(yhdE)	Complementary strand	3397298	3397891
JW3218(mreD)	Complementary strand	3397900	3398388
JW3219(mreC)	Complementary strand	3398388	3399491
JW3220(mreB)	Complementary strand	3399557	3400600
JW3221(yhdA)	Complementary strand	3400905	3402845
JW3222(yhdH)		3402997	3403971
JW3222.5(529#3)		3404788	3404976
JW3223(accB)		3404949	3405419
JW3224(accC)		3405430	3406779
JW3225(yhdT)		3406888	3407130
JW3226(panF)		3407120	3408571
JW3227(prmA)		3408583	3409464
JW3228(yhdG)		3409793	3410758
JW3229(fis)		3410784	3411080
JW3230(yhdJ)		3411160	3412050
JW3231(yhdU)		3412134	3412313
JW3232(envR)	Complementary strand	3412316	3412978
JW3233(acrE)		3413377	3414534
JW3234(envD)		3414546	3417650
JW3235(yhdV)		3417903	3418124
JW3236(yhdW)		3418662	3419579
JW3237(yhdX)		3419629	3420735
JW3238(yhdY)		3420834	3421940
JW3239(yhdZ)		3421948	3422706
JW3240(yrdA)		3428533	3429303
JW3241(yrdB)	Complementary strand	3429279	3429536
JW3242(aroE)	Complementary strand	3429533	3430351
JW3243(yrdC)	Complementary strand	3430356	3430928
JW3244(yrdD)	Complementary strand	3430933	3431442
JW3245(smg)	Complementary strand	3431505	3431978
JW3246(smf)	Complementary strand	3431950	3432327
JW3247(smf)	Complementary strand	3432312	3433073
JW3248(def)		3433203	3433712
JW3249(fmt)		3433727	3434674
JW3250(sun)		3434720	3436009
JW3251(trkA)		3436031	3437407
JW3252(mscL)		3437537	3437947
JW3253(yhdL)	Complementary strand	3437944	3438162
JW3254(zntR)	Complementary strand	3438218	3438643
JW3255(yhdN)	Complementary strand	3438654	3439022
JW3256(rplQ)	Complementary strand	3439129	3439512
JW3257(rpoA)	Complementary strand	3439553	3440542
JW3258(rpsD)	Complementary strand	3440568	3441188
JW3259(rpsK)	Complementary strand	3441222	3441611
JW3260(rpsM)	Complementary strand	3441628	3441984
JW3261(rpmJ)	Complementary strand	3442131	3442247
JW3262(secY)	Complementary strand	3442279	3443610
JW3263(rplO)	Complementary strand	3443618	3444052
JW3264(rpmD)	Complementary strand	3444056	3444235
JW3265(rpsE)	Complementary strand	3444239	3444742
JW3266(rplR)	Complementary strand	3444757	3445110
JW3267(rplF)	Complementary strand	3445120	3445653
JW3268(rpsH)	Complementary strand	3445666	3446058
JW3269(rpsN)	Complementary strand	3446092	3446397
JW3270(rplE)	Complementary strand	3446412	3446951
JW3271(rplX)	Complementary strand	3446966	3447280
JW3272(rplN)	Complementary strand	3447291	3447662
JW3273(rpsQ)	Complementary strand	3447827	3448081
JW3274(rpmC)	Complementary strand	3448081	3448272
JW3275(rplP)	Complementary strand	3448272	3448682
JW3276(rpsC)	Complementary strand	3448695	3449396
JW3277(rplV)	Complementary strand	3449414	3449746
JW3278(rpsS)	Complementary strand	3449761	3450039
JW3279(rplB)	Complementary strand	3450056	3450877
JW3280(rplW)	Complementary strand	3450895	3451197
JW3281(rplD)	Complementary strand	3451194	3451799
JW3282(rplC)	Complementary strand	3451810	3452439
JW3283(rpsJ)	Complementary strand	3452472	3452783
JW3284(pinO)	Complementary strand	3453021	3453440
JW3285(gspA)	Complementary strand	3453442	3454911
JW3286(gspC)		3455091	3455906
JW3287(gspD)		3455878	3457842
JW3288(gspE)		3457852	3459333
JW3289(hofF)		3459330	3460526
JW3290(hofG)		3460536	3460973
JW3291(hofH)		3460981	3461490
JW3292(gspI)		3461448	3461864
JW3293(gspJ)		3461857	3462444
JW3294(gspK)		3462437	3463420
JW3295(gspL)		3463432	3464598
JW3296(pshM)		3464571	3465056
JW3297(hofD)		3465056	3465733
JW3298(bfr)	Complementary strand	3465762	3466238
JW3299(yheA)	Complementary strand	3466310	3466504
JW3300(chiA)	Complementary strand	3466673	3469366
JW3301(tufA)	Complementary strand	3469658	3470842
JW3302(fusA)	Complementary strand	3470913	3473027
JW3303(rpsG)	Complementary strand	3473055	3473594
JW3304(rpsL)	Complementary strand	3473691	3474065
JW3305(yheL)	Complementary strand	3474191	3474478
JW3306(yheM)	Complementary strand	3474486	3474845
JW3307(yheN)	Complementary strand	3474845	3475231
JW3308(yheO)	Complementary strand	3475231	3475965
JW3309(fkpA)	Complementary strand	3476120	3476932
JW3310(slyX)		3477153	3477371
JW3311(slyD)	Complementary strand	3477420	3478010
JW3312(625#3)	Complementary strand	3478105	3478305
JW3313(kefB)	Complementary strand	3478315	3480120
JW3314(yheR)	Complementary strand	3480120	3480674
JW3315(yheS)		3480802	3482715
JW3316(yheT)		3482715	3483737
JW3317(yheU)		3483731	3483949
JW3318(prkB)		3484003	3484872
JW3319(yhfA)	Complementary strand	3484927	3485331
JW3320(crp)		3485633	3486265
JW3321(yhfK)		3486316	3488406
JW3322(argD)	Complementary strand	3488473	3489693
JW3323(pabA)	Complementary strand	3489779	3490342
JW3324(fic)	Complementary strand	3490374	3490976
JW3325(yhfG)	Complementary strand	3490966	3491133
JW3326(ppiA)	Complementary strand	3491238	3491810
JW3327(yhfC)		3492081	3493262
JW3328(nirB)		3493524	3496067
JW3329(nirD)		3496064	3496390
JW3330(nirC)		3496516	3497322
JW3331(cysG)		3497341	3498714
JW3332(yhfL)		3498961	3499128
JW3333(yhfM)		3499423	3500760
JW3334(yhfN)		3500760	3501803
JW3335(yhfO)		3501766	3502215
JW3336(yhfP)		3502280	3502684
JW3337(yhfQ)		3502681	3503466
JW3338(yhfR)		3503500	3504297
JW3339(yhfS)	Complementary strand	3504449	3505534
JW3340(yhfT)	Complementary strand	3505546	3506850
JW3341(yhfU)	Complementary strand	3506862	3507254
JW3342(php)	Complementary strand	3507226	3508104
JW3343(yhfW)	Complementary strand	3508101	3509327
JW3344(yhfX)	Complementary strand	3509327	3510490
JW3345(yhfY)	Complementary strand	3510574	3510978
JW3346(yhfZ)	Complementary strand	3510953	3511639
JW3347(trpS)	Complementary strand	3512148	3513152
JW3348(gph)	Complementary strand	3513145	3513903
JW3349(rpe)	Complementary strand	3513896	3514573
JW3350(dam)	Complementary strand	3514591	3515427
JW3351(damX)	Complementary strand	3515534	3516820
JW3352(aroB)	Complementary strand	3516912	3518000
JW3353(aroK)	Complementary strand	3518057	3518578
JW3354(hofQ)	Complementary strand	3518979	3520217
JW3355(yrfA)	Complementary strand	3520129	3520572
JW3356(yrfB)	Complementary strand	3520523	3520963
JW3357(yrfC)	Complementary strand	3520947	3521486
JW3358(yrfD)	Complementary strand	3521486	3522292
JW3359(mrcA)		3522385	3524937
JW3360(yrfE)	Complementary strand	3525103	3525663
JW3361(yrfF)		3525983	3528118
JW3362(yrfG)		3528138	3528851
JW3363(yrfH)		3528862	3529263
JW3364(yrfI)		3529282	3530166
JW3365(yhgE)	Complementary strand	3530229	3531953
JW3366(pckA)		3532332	3533954
JW3367(envZ)	Complementary strand	3534030	3535382
JW3368(ompR)	Complementary strand	3535379	3536098
JW3369(greB)		3536326	3536802
JW3370(yhgF)		3536899	3539220
JW3371(feoA)		3539675	3539902
JW3372(feoB)		3539919	3542240
JW3373(yhgG)		3542240	3542476
JW3374(yhgA)		3542679	3543557
JW3375(bioH)	Complementary strand	3543586	3544356
JW3376(yhgH)		3544346	3545077
JW3377(yhgI)		3545136	3545711
JW3378(gntT)		3546071	3547384
JW3379(malQ)	Complementary strand	3547495	3549579
JW3380(malP)	Complementary strand	3549589	3551982
JW3381(malT)		3552594	3555299
JW3382(rtcA)	Complementary strand	3555342	3555947
JW3383(rtcA)	Complementary strand	3555920	3556360
JW3384(rtcB)	Complementary strand	3556361	3557587
JW3385(rtcR)		3557776	3559374
JW3386(glpR)	Complementary strand	3559356	3560114
JW3387(glpG)	Complementary strand	3560131	3560961
JW3388(glpE)	Complementary strand	3561006	3561332
JW3389(glpD)		3561522	3563027
JW3390(yzgL)	Complementary strand	3563233	3563514
JW3391(glgP)	Complementary strand	3563643	3566090
JW3392(glgA)	Complementary strand	3566109	3567542
JW3393(glgC)	Complementary strand	3567542	3568837
JW3394(glgX)	Complementary strand	3568855	3570828
JW3395(glgB)	Complementary strand	3570825	3573011
JW3396(asd)	Complementary strand	3573284	3574387
JW3397(yhgN)		3574580	3575173
JW3398(gntU)	Complementary strand	3575230	3575565
JW3399(gntU)	Complementary strand	3575571	3576569
JW3400(gntK)	Complementary strand	3576573	3577100
JW3401(gntR)	Complementary strand	3577292	3578233
JW3402(yhhW)	Complementary strand	3578457	3579152
JW3403(yhhX)	Complementary strand	3579275	3580312
JW3404(615#7.1)		3580259	3580411
JW3405(yhhY)		3580645	3581133
JW3406(yhhZ)		3581370	3582548
JW3407(yrhA)		3582545	3582961
JW3408(insA)		3582990	3583265
JW3409(insB)		3583184	3583687
JW3410(615#10.1)		3583950	3584066
JW3411(yrhB)		3584266	3584550
JW3412(ggt)	Complementary strand	3584588	3586330
JW3413(yhhA)		3586450	3586890
JW3414(ugpQ)	Complementary strand	3586877	3587620
JW3415(ugpC)	Complementary strand	3587617	3588687
JW3416(ugpE)	Complementary strand	3588689	3589534
JW3417(ugpA)	Complementary strand	3589531	3590418
JW3418(ugpB)	Complementary strand	3590516	3591832
JW3418.5(613#2)	Complementary strand	3592067	3592219
JW3419(livF)	Complementary strand	3592231	3592944
JW3420(livG)	Complementary strand	3592946	3593713
JW3421(livM)	Complementary strand	3593710	3594987
JW3422(livH)	Complementary strand	3594984	3595910
JW3423(livK)	Complementary strand	3595958	3597067
JW3424(yhhK)		3597491	3597874
JW3425(livJ)	Complementary strand	3598062	3599165
JW3426(rpoH)	Complementary strand	3599436	3600290
JW3427(ftsX)	Complementary strand	3600535	3601593
JW3428(ftsE)	Complementary strand	3601586	3602254
JW3429(ftsY)	Complementary strand	3602257	3603750
JW3430(yhhF)		3603900	3604496
JW3431(yhhL)		3604363	3604755
JW3432(yhhM)	Complementary strand	3604758	3605117
JW3433(yhhN)		3605258	3605884
JW3434(atzN)		3605958	3608156
JW3435(yhhP)	Complementary strand	3608258	3608503
JW3436(yhhQ)		3608724	3609389
JW3437(yhhR)		3609408	3610019
JW3438(yhhS)	Complementary strand	3610023	3611282
JW3439(yhhT)		3611291	3612421
JW3440(yhhU)		3612476	3613063
JW3441(nikA)		3613174	3614748
JW3442(nikB)		3614748	3615692
JW3443(nikC)		3615689	3616522
JW3444(nikD)		3616522	3617286
JW3445(nikE)		3617283	3618089
JW3446(yhhG)		3618095	3618496
JW3447(rhsB)		3618699	3622934
JW3448(610#6.1)	Complementary strand	3621225	3621365
JW3449(yhhH)		3622906	3623289
JW3450(yibA)		3623379	3623639
JW3451(yhhI)		3623885	3625021
JW3452(yhhJ)	Complementary strand	3625186	3626313
JW3453(yhiH)	Complementary strand	3626310	3628994
JW3454(yhiI)	Complementary strand	3629041	3630108
JW3455(yhiJ)	Complementary strand	3630474	3632096
JW3456(yhiK)	Complementary strand	3632358	3632729
JW3457(yhiL)	Complementary strand	3632726	3633964
JW3458(yhiM)		3634248	3635399
JW3459(yhiN)	Complementary strand	3635714	3636916
JW3460(pitA)		3637148	3638647
JW3461(uspB)	Complementary strand	3638891	3639226
JW3462(uspA)		3639617	3640051
JW3463(yhiP)		3640368	3641837
JW3464(yhiQ)	Complementary strand	3641886	3642743
JW3465(prlC)	Complementary strand	3642646	3644688
JW3466(yhiR)		3644891	3645733
JW3467(gor)		3645805	3647157
JW3468(arsR)		3648034	3648387
JW3469(arsB)		3648441	3649730
JW3470(arsC)		3649743	3650168
JW3471(yhiS)		3650797	3651579
JW3472(is5B)	Complementary strand	3651688	3652704
JW3473(yi51)		3652024	3652392
JW3474(slp)		3653467	3654033
JW3475(yhiF)		3654189	3654719
JW3476(yhiD)	Complementary strand	3654761	3655408
JW3477(hdeB)	Complementary strand	3655472	3655810
JW3478(hdeA)	Complementary strand	3655914	3656246
JW3479(hdeD)		3656501	3657073
JW3480(yhiE)		3657872	3658399
JW3481(yhiU)		3658738	3659895
JW3482(yhiV)		3659920	3663033
JW3483(yhiW)	Complementary strand	3663396	3664124
JW3484(yhiX)	Complementary strand	3664492	3665316
JW3485(gadA)	Complementary strand	3665686	3667086
JW3486(yhjA)	Complementary strand	3667297	3668694
JW3487(treF)		3669098	3670747
JW3488(yhjB)	Complementary strand	3670798	3671400
JW3489(yhjC)		3671848	3672819
JW3490(yhjD)		3672868	3673881
JW3491(yhjE)		3674292	3675614
JW3492(yhjG)	Complementary strand	3675796	3677871
JW3493(yhjH)	Complementary strand	3677926	3678693
JW3494(kdgK)		3678706	3679854
JW3495(yhjJ)	Complementary strand	3679950	3681446
JW3496(dctA)	Complementary strand	3681667	3682953
JW3497(yhjK)	Complementary strand	3683136	3685091
JW3498(yhjL)	Complementary strand	3685206	3688628
JW3499(yhjM)	Complementary strand	3688660	3689766
JW3500(yhjN)	Complementary strand	3689773	3692112
JW3501(yhjO)	Complementary strand	3692123	3694789
JW3502(yhjQ)	Complementary strand	3694738	3695466
JW3503(yhjR)	Complementary strand	3695502	3695690
JW3504(yhjS)		3695963	3697534
JW3505(yhjT)		3697534	3697722
JW3506(yhjU)		3697719	3699398
JW3507(603#8)	Complementary strand	3699485	3699760
JW3508(yhjV)		3700068	3701339
JW3509(dppF)	Complementary strand	3701369	3702373
JW3510(dppD)	Complementary strand	3702370	3703353
JW3511(dppC)	Complementary strand	3703364	3704266
JW3512(dppB)	Complementary strand	3704276	3705295
JW3513(dppA)	Complementary strand	3705603	3707210
JW3514(yzpK)	Complementary strand	3707725	3708072
JW3515(yhjW)	Complementary strand	3708289	3710013
JW3516(yhjX)	Complementary strand	3710304	3711512
JW3517(yhjY)	Complementary strand	3711741	3712445
JW3518(tag)		3712597	3713160
JW3519(yiaC)		3713157	3713597
JW3520(bisC)	Complementary strand	3713566	3715905
JW3521(yiaD)		3716052	3716711
JW3522(yiaE)		3716803	3717789
JW3523(yiaF)	Complementary strand	3717839	3718669
JW3524(yiaG)		3718983	3719273
JW3525(cspA)		3719554	3719766
JW3526(yiaZ)	Complementary strand	3719953	3720105
JW3527(yi5A)		3720185	3720706
JW3528(yi5B)		3720703	3721554
JW3529(yi5C)	Complementary strand	3720961	3721317
JW3530(glyS)	Complementary strand	3721833	3723902
JW3531(glyQ)	Complementary strand	3723912	3724823
JW3532(601#3)	Complementary strand	3724918	3725217
JW3533(yiaH)		3725392	3726387
JW3534(yiaA)	Complementary strand	3726429	3726866
JW3535(yiaB)	Complementary strand	3726912	3727265
JW3536(xylB)	Complementary strand	3727422	3728876
JW3537(xylA)	Complementary strand	3728948	3730270
JW3538(xylF)		3730636	3731628
JW3539(xylG)		3731706	3733247
JW3540(xylH)		3733225	3734406
JW3541(xylR)		3734484	3735662
JW3542(bax)	Complementary strand	3735858	3736682
JW3543(malS)		3737002	3739032
JW3544(avtA)		3739210	3740463
JW3545(ysaA)	Complementary strand	3740614	3741087
JW3546(yiaJ)	Complementary strand	3741189	3742037
JW3547(yiaK)		3742238	3743236
JW3548(yiaL)		3743248	3743715
JW3549(yiaM)		3743833	3744306
JW3550(yiaN)		3744309	3745583
JW3551(yiaO)		3745600	3746586
JW3552(lyx)		3746590	3748086
JW3553(sgbH)		3748083	3748745
JW3554(sgbU)		3748705	3749598
JW3555(sgbE)		3749592	3750287
JW3556(yiaT)	Complementary strand	3750634	3751374
JW3557(yiaU)		3751498	3752472
JW3558(yiaV)	Complementary strand	3752469	3753605
JW3559(yiaW)	Complementary strand	3753611	3753934
JW3560(577#1.1)		3754048	3754260
JW3561(aldB)	Complementary strand	3754479	3756017
JW3562(yiaY)	Complementary strand	3756182	3757330
JW3563(selB)	Complementary strand	3757520	3759364
JW3564(selA)	Complementary strand	3759361	3760752
JW3565(yibF)	Complementary strand	3760850	3761458
JW3566(rhsA)		3761686	3765819
JW3567(576#2.1)	Complementary strand	3764212	3764631
JW3568(yibA)		3765840	3766682
JW3569(yibJ)		3766724	3767425
JW3570(yibG)		3767680	3768141
JW3570.5(576#7)		3769491	3769649
JW3571(yibH)	Complementary strand	3769746	3770882
JW3572(yibI)	Complementary strand	3770885	3771247
JW3573(mtlA)		3771784	3773697
JW3574(mtlD)		3773927	3775075
JW3575(mtlR)		3775075	3775662
JW3576(576#14)	Complementary strand	3775674	3775883
JW3577(yibL)		3776168	3776530
JW3578(lldP)		3776902	3778557
JW3579(lldR)		3778557	3779333
JW3580(lldD)		3779330	3780520
JW3581(yibK)		3780718	3781191
JW3582(cysE)	Complementary strand	3781244	3782065
JW3583(gpsA)	Complementary strand	3782145	3783164
JW3584(secB)	Complementary strand	3783164	3783631
JW3585(grxC)	Complementary strand	3783694	3783945
JW3586(yibN)	Complementary strand	3784087	3784518
JW3587(yibO)		3784763	3786307
JW3588(yibP)		3786317	3787600
JW3589(yibQ)		3787730	3788563
JW3590(yibD)	Complementary strand	3788550	3789584
JW3591(tdh)	Complementary strand	3789823	3790848
JW3592(kbl)	Complementary strand	3790858	3792054
JW3593(htrL)	Complementary strand	3792329	3793201
JW3594(rfaD)		3793490	3794422
JW3595(rfaF)		3794432	3795478
JW3596(rfaC)		3795482	3796441
JW3597(rfaL)		3796451	3797710
JW3598(rfaK)	Complementary strand	3797742	3798815
JW3599(rfaZ)	Complementary strand	3798848	3799699
JW3600(rfaY)	Complementary strand	3799770	3800468
JW3601(rfaJ)	Complementary strand	3800486	3801502
JW3602(rfaI)	Complementary strand	3801542	3802561
JW3603(rfaB)	Complementary strand	3802561	3803670
JW3604(rfaS)	Complementary strand	3803684	3804619
JW3605(rfaP)	Complementary strand	3804656	3805453
JW3606(rfaG)	Complementary strand	3805446	3806570
JW3607(rfaQ)	Complementary strand	3806567	3807601
JW3608(kdtA)		3808043	3809320
JW3609(kdtB)		3809328	3809807
JW3610(mutM)	Complementary strand	3809846	3810655
JW3611(rpmG)	Complementary strand	3810753	3810920
JW3612(rpmB)	Complementary strand	3810941	3811177
JW3613(radC)	Complementary strand	3811394	3812068
JW3614(dfp)		3812162	3813454
JW3615(dut)		3813435	3813890
JW3616(ttk)		3813955	3814593
JW3617(pyrE)	Complementary strand	3814630	3815271
JW3618(rph)	Complementary strand	3815366	3816052
JW3619(yicC)		3816179	3817042
JW3620(dinD)		3817263	3818087
JW3621(yicG)		3818377	3818994
JW3622(yicF)	Complementary strand	3818991	3820673
JW3623(gmk)		3820931	3821554
JW3624(rpoZ)		3821609	3821884
JW3625(spoT)		3821903	3824011
JW3626(spoU)		3824018	3824707
JW3627(recG)		3824713	3826794
JW3628(gltS)	Complementary strand	3826963	3828168
JW3629(yicE)		3828448	3829839
JW3630(yicH)		3829960	3831669
JW3631(yicI)	Complementary strand	3831722	3834040
JW3632(yicJ)	Complementary strand	3834050	3835432
JW3633(yicK)		3836456	3837640
JW3634(yicL)		3837751	3838674
JW3635(nlpA)	Complementary strand	3838678	3839496
JW3636.1(569#3.2)		3839718	3840011
JW3637(yicM)	Complementary strand	3840052	3841290
JW3638(yicN)	Complementary strand	3841453	3841932
JW3639(yicO)	Complementary strand	3841958	3843370
JW3640(yicP)		3843467	3845233
JW3641(uhpT)	Complementary strand	3845279	3846670
JW3642(uhpC)	Complementary strand	3846808	3848127
JW3643(uhpB)	Complementary strand	3848137	3849639
JW3644(uhpA)	Complementary strand	3849639	3850229
JW3645(ilvN)	Complementary strand	3850305	3850595
JW3646(ilvB)	Complementary strand	3850599	3852287
JW3647(ivbL)	Complementary strand	3852393	3852491
JW3648(567#1)		3853056	3853145
JW3649(emrD)		3853419	3854609
JW3650(yidF)	Complementary strand	3854617	3855114
JW3651(yidG)	Complementary strand	3855111	3855473
JW3652(yidH)	Complementary strand	3855463	3855810
JW3653(yidI)		3855918	3856367
JW3654(yidJ)	Complementary strand	3856414	3857907
JW3655(yidK)	Complementary strand	3857904	3859619
JW3656(yidL)		3859756	3860679
JW3657(567#9.1)	Complementary strand	3860676	3860888
JW3658(glvG)	Complementary strand	3860852	3861490
JW3659(glvB)	Complementary strand	3861490	3861975
JW3660(glvC)	Complementary strand	3862000	3863106
JW3661(yidP)		3863402	3864118
JW3662(yidE)	Complementary strand	3864115	3865776
JW3663(ibpB)	Complementary strand	3865972	3866400
JW3664(ibpA)	Complementary strand	3866512	3866925
JW3665(yidQ)		3867156	3867563
JW3666(yidR)	Complementary strand	3867565	3868815
JW3667(yidS)		3868859	3869944
JW3668(dgoT)	Complementary strand	3869941	3871278
JW3669(dgoA)	Complementary strand	3871353	3873116
JW3670(dgoK)	Complementary strand	3873100	3873978
JW3671(dgoR)	Complementary strand	3873975	3874271
JW3672(yidW)	Complementary strand	3874277	3874663
JW3673(yidX)		3874998	3875669
JW3674(yidA)	Complementary strand	3875644	3876456
JW3675(yidB)	Complementary strand	3876571	3876969
JW3676(gyrB)	Complementary strand	3877209	3879623
JW3677(recF)	Complementary strand	3879652	3880725
JW3678(dnaN)	Complementary strand	3880725	3881825
JW3679(dnaA)	Complementary strand	3881830	3883233
JW3680(rpmH)		3883841	3883981
JW3681(rnpA)		3883998	3884357
JW3682(yidD)		3884321	3884578
JW3683(yidC)		3884581	3886227
JW3684(thdF)		3886333	3887697
JW3685(tnaL)		3887940	3888014
JW3686(tnaA)		3888235	3889650
JW3687(tnaB)		3889741	3890988
JW3688(yidY)		3891120	3892295
JW3689(yidZ)		3892270	3893229
JW3690(yieE)		3893374	3894135
JW3691(yieF)		3894157	3894723
JW3692(yieG)	Complementary strand	3894777	3896114
JW3693(yieH)		3896279	3896944
JW3694(yieI)		3897011	3897478
JW3695(yieJ)		3897527	3898114
JW3696(yieK)	Complementary strand	3898187	3898828
JW3697(yieL)	Complementary strand	3898912	3900114
JW3698(yieC)	Complementary strand	3900108	3901724
JW3699(bglB)	Complementary strand	3901793	3903205
JW3700(bglF)	Complementary strand	3903224	3905101
JW3701(bglG)	Complementary strand	3905235	3906071
JW3702(phoU)	Complementary strand	3906357	3907082
JW3703(pstB)	Complementary strand	3907097	3907870
JW3704(pstA)	Complementary strand	3908053	3908943
JW3705(pstC)	Complementary strand	3908943	3909902
JW3706(pstS)	Complementary strand	3909989	3911029
JW3707(glmS)	Complementary strand	3911343	3913172
JW3708(glmU)	Complementary strand	3913334	3914704
JW3709(atpC)	Complementary strand	3915057	3915476
JW3710(uncD)	Complementary strand	3915497	3916879
JW3711(atpG)	Complementary strand	3916906	3917769
JW3712(atpA)	Complementary strand	3917820	3919361
JW3713(atpH)	Complementary strand	3919374	3919907
JW3714(atpF)	Complementary strand	3919922	3920392
JW3715(atpE)	Complementary strand	3920454	3920693
JW3716(atpB)	Complementary strand	3920740	3921555
JW3717(atpI)	Complementary strand	3921564	3921956
JW3718(gidB)	Complementary strand	3922561	3923184
JW3719(gidA)	Complementary strand	3923248	3925137
JW3720(mioC)	Complementary strand	3925516	3925959
JW3721(asnC)	Complementary strand	3926049	3926507
JW3722(asnA)		3926659	3927651
JW3723(yieM)	Complementary strand	3927656	3928939
JW3724(559#4)	Complementary strand	3928924	3929106
JW3725(yieN)	Complementary strand	3929100	3930596
JW3726(kup)		3930819	3932378
JW3727(rbsD)		3932817	3933272
JW3728(rbsA)		3933280	3934785
JW3729(rbsC)		3934790	3935755
JW3730(rbsB)		3935780	3936670
JW3731(rbsK)		3936796	3937725
JW3732(rbsR)		3937729	3938721
JW3733(yieO)	Complementary strand	3938687	3940114
JW3734(yieP)	Complementary strand	3940137	3940682
JW3735(yifA)	Complementary strand	3946628	3947224
JW3736(pssR)	Complementary strand	3947065	3947466
JW3737(yifE)		3947585	3947923
JW3738(yifB)	Complementary strand	3947948	3949498
JW3739(ilvL)		3949821	3949919
JW3740(ilvG)		3950059	3951042
JW3741(ilvG)		3951122	3951703
JW3742(ilvM)		3951700	3951963
JW3743(ilvE)		3951983	3952912
JW3744(ilvD)		3953008	3954825
JW3745(ilvA)		3954828	3956372
JW3746(ilvY)	Complementary strand	3956424	3957317
JW3747(ilvC)		3957467	3958942
JW3748(ppiC)	Complementary strand	3959026	3959307
JW3749(yifN)	Complementary strand	3959503	3959778
JW3750(yifN)	Complementary strand	3959733	3959951
JW3751(rep)		3960168	3962189
JW3752(gppA)	Complementary strand	3962236	3963720
JW3753(rhlB)	Complementary strand	3963856	3965121
JW3754(trxA)		3965252	3965581
JW3755(rhoL)		3965722	3965823
JW3756(rho)		3965908	3967167
JW3757(555#2.1)		3967177	3967395
JW3758(rfe)		3967407	3968510
JW3759(wzzE)		3968519	3969568
JW3760(nfrC)		3969624	3970751
JW3761(rffD)		3970748	3972010
JW3762(rffG)		3972010	3973077
JW3763(rffH)		3973096	3973977
JW3764(yifH)		3974084	3974629
JW3765(rffA)		3974634	3975764
JW3766(wzxE)		3975766	3977016
JW3767(554#5.1)		3977013	3977237
JW3768(rffT)		3977152	3978093
JW3769(rffT)		3978090	3979442
JW3770(wecG)		3979445	3980185
JW3771(yifK)		3980376	3981761
JW3772(aslB)		3982447	3983682
JW3773(aslA)	Complementary strand	3983841	3985496
JW3774(hemY)	Complementary strand	3986175	3987371
JW3775(hemX)	Complementary strand	3987374	3988555
JW3776(hemD)	Complementary strand	3988577	3989317
JW3777(hemC)	Complementary strand	3989314	3990255
JW3778(cyaA)		3990642	3993188
JW3779(cyaY)	Complementary strand	3993228	3993548
JW3780(yzcX)		3993339	3993824
JW3781(yifL)		3994011	3994214
JW3782(dapF)		3994248	3995075
JW3783(yigA)		3995072	3995779
JW3784(xerC)		3995776	3996672
JW3785(yigB)		3996672	3997388
JW3786(uvrD)		3997472	3999634
JW3787(yigE)	Complementary strand	3999781	4000158
JW3788(yigE)	Complementary strand	4000059	4000544
JW3789(corA)		4000914	4001864
JW3790(yigF)	Complementary strand	4001907	4002287
JW3791(yigG)	Complementary strand	4002301	4002717
JW3792(radD)	Complementary strand	4002776	4003678
JW3793(yigH)	Complementary strand	4003717	4004202
JW3794(pldA)		4004349	4005218
JW3795(recQ)		4005345	4007177
JW3796(yigJ)		4007355	4007522
JW3797(yigJ)		4007393	4007860
JW3798(yigK)	Complementary strand	4007922	4008338
JW3799(551#4)	Complementary strand	4008335	4008541
JW3800(pldB)		4008652	4009674
JW3801(yigL)		4009556	4009822
JW3802(yigL)		4009794	4010309
JW3803(yigM)		4010542	4011441
JW3804(metR)	Complementary strand	4011329	4012282
JW3805(metE)		4012519	4014780
JW3806(ysgA)	Complementary strand	4014609	4015433
JW3807(551#11.1)	Complementary strand	4014820	4014993
JW3808(udp)		4015894	4016655
JW3809(yigN)		4016796	4018223
JW3810(ubiE)		4018318	4019073
JW3811(yigP)		4019087	4019692
JW3812(aarF)		4019689	4021329
JW3813(yigT)		4021408	4021677
JW3814(yigT)		4021761	4022198
JW3815(tatC)		4022201	4022977
JW3816(tatD)		4023007	4023627
JW3817(yigW)		4023554	4023802
JW3818(rfaH)	Complementary strand	4023799	4024287
JW3819(ubiD)		4024454	4025947
JW3820(ubiB)		4025993	4026694
JW3821(fadA)	Complementary strand	4027075	4028238
JW3822(fadB)	Complementary strand	4028248	4030437
JW3823(pepQ)		4030627	4031958
JW3824(yigZ)		4031955	4032572
JW3825(trkH)		4032611	4033909
JW3826(trkH)		4033867	4034061
JW3827(hemG)		4034073	4034618
JW3828(mobB)	Complementary strand	4040362	4040889
JW3829(mobA)	Complementary strand	4040871	4041455
JW3830(yihD)		4041525	4041794
JW3831(yihE)		4041872	4042858
JW3832(dsbA)		4042875	4043501
JW3833(yihF)		4043613	4045085
JW3834(yihG)	Complementary strand	4045126	4046058
JW3835(polA)		4046422	4049208
JW3836(yihA)	Complementary strand	4049589	4050188
JW3837(yihI)		4050803	4051312
JW3838(hemN)		4051501	4052874
JW3839(ntrC)	Complementary strand	4053325	4054734
JW3840(ntrB)	Complementary strand	4054746	4055795
JW3841(glnA)	Complementary strand	4056081	4057490
JW3842(yihK)		4057863	4059638
JW3843(yihL)		4059902	4060612
JW3844(yihM)		4060620	4061600
JW3845(yihN)		4061702	4062967
JW3846(yshA)	Complementary strand	4063058	4063750
JW3847(yihO)	Complementary strand	4063765	4065228
JW3848(yihP)	Complementary strand	4065265	4066671
JW3849(yihQ)	Complementary strand	4066696	4068732
JW3850(yihR)	Complementary strand	4068931	4069857
JW3851(yihS)	Complementary strand	4069971	4071227
JW3852(yihT)	Complementary strand	4071229	4072107
JW3853(yihU)	Complementary strand	4072131	4073027
JW3854(yihV)		4073189	4074091
JW3855(yihW)		4074101	4074910
JW3856(yihX)		4074988	4075608
JW3857(rbn)		4075602	4076474
JW3858(yihZ)		4076471	4076908
JW3859(yiiD)		4076905	4077894
JW3860(yiiE)		4078720	4078965
JW3861(yiiF)		4079183	4079425
JW3862(fdhE)	Complementary strand	4079755	4080684
JW3863(fdoI)	Complementary strand	4080681	4081316
JW3864(fdoH)	Complementary strand	4081313	4082215
JW3865(fdoG)	Complementary strand	4082228	4085278
JW3866(fdhD)		4085472	4086305
JW3867(yiiG)		4086458	4087513
JW3868(frvR)	Complementary strand	4087564	4089312
JW3869(frvX)	Complementary strand	4089312	4090382
JW3870(frvB)	Complementary strand	4090372	4091829
JW3871(frvA)	Complementary strand	4091834	4092280
JW3872(yiiL)	Complementary strand	4092581	4092895
JW3873(rhaD)	Complementary strand	4092905	4093729
JW3874(rhaA)	Complementary strand	4094180	4095439
JW3875(rhaB)	Complementary strand	4095436	4096905
JW3876(rhaS)		4097193	4098029
JW3877(rhaR)		4098013	4098951
JW3878(rhaT)	Complementary strand	4098948	4099982
JW3879(sodA)		4100267	4100887
JW3880(kdgT)		4101138	4102130
JW3881(yiiM)		4102249	4102953
JW3882(cpxA)	Complementary strand	4103059	4104432
JW3883(cpxR)	Complementary strand	4104429	4105127
JW3884(540#3.1)		4105274	4105423
JW3885(cpxP)		4105408	4105776
JW3886(yiiP)		4105925	4106827
JW3887(pfkA)		4107008	4107970
JW3888(sbp)		4108290	4109279
JW3889(cdh)		4109386	4110141
JW3890(tpiA)	Complementary strand	4110196	4110963
JW3891(yiiQ)	Complementary strand	4111071	4111670
JW3892(yiiR)		4111771	4112211
JW3893(yiiS)		4112423	4112722
JW3894(yiiT)		4112749	4113177
JW3895(fpr)	Complementary strand	4113182	4113928
JW3896(glpX)	Complementary strand	4114025	4115035
JW3897(glpK)	Complementary strand	4115170	4116678
JW3898(glpF)	Complementary strand	4116701	4117546
JW3899(yiiU)		4117971	4118216
JW3900(menG)	Complementary strand	4118301	4118786
JW3901(menA)	Complementary strand	4118879	4119805
JW3902(hslU)	Complementary strand	4119872	4121203
JW3903(hslV)	Complementary strand	4121213	4121743
JW3904(ftsN)	Complementary strand	4121836	4122795
JW3905(cytR)	Complementary strand	4122887	4123912
JW3906(priA)	Complementary strand	4124068	4126266
JW3907(rpmE)		4126469	4126681
JW3908(yiiX)	Complementary strand	4126742	4127350
JW3909(metJ)	Complementary strand	4127534	4127851
JW3910(metB)		4128128	4129288
JW3911(metL)		4129291	4131723
JW3912(537#1.1)	Complementary strand	4131862	4132410
JW3913(metF)		4132072	4132962
JW3914(katG)		4133291	4135471
JW3915(yijE)		4135531	4136469
JW3916(yijF)	Complementary strand	4136496	4137113
JW3917(gldA)	Complementary strand	4137388	4138530
JW3918(talC)	Complementary strand	4138502	4139164
JW3919(ptsA)	Complementary strand	4139176	4141311
JW3920(536#1)		4141169	4141642
JW3921(frwC)		4141985	4143064
JW3922(frwB)		4143079	4143399
JW3923(pflD)		4143450	4145747
JW3924(pflC)		4145713	4146591
JW3925(frwD)		4146593	4146934
JW3926(yijO)	Complementary strand	4146921	4147772
JW3927(yijP)	Complementary strand	4147987	4149720
JW3928(ppc)	Complementary strand	4149902	4152553
JW3929(argE)	Complementary strand	4153151	4154302
JW3930(argC)		4154456	4155460
JW3931(argB)		4155468	4156244
JW3932(argH)		4156305	4157678
JW3933(oxyR)		4157945	4158862
JW3934(udhA)	Complementary strand	4158845	4160179
JW3935(yijC)		4160522	4161226
JW3936(yijD)		4161226	4161585
JW3937(trmA)	Complementary strand	4161625	4162725
JW3938(btuB)		4163094	4164938
JW3939(murI)		4164871	4165740
JW3940(murB)		4171512	4172540
JW3941(birA)		4172537	4173502
JW3942(coaA)	Complementary strand	4173531	4174481
JW3943(tufB)		4175399	4176583
JW3944(secE)		4176813	4177196
JW3945(nusG)		4177198	4177743
JW3946(rplK)		4177901	4178329
JW3947(rplA)		4178333	4179037
JW3948(rplJ)		4179450	4179947
JW3949(rplL)		4180014	4180379
JW3950(rpoB)		4180699	4184727
JW3951(rpoC)		4184804	4189027
JW3952(htrC)		4189240	4189779
JW3953(thiH)	Complementary strand	4190189	4191322
JW3954(thiG)	Complementary strand	4191319	4192164
JW3955(thiS)	Complementary strand	4192091	4192291
JW3956(thiF)	Complementary strand	4192275	4193030
JW3957(thiE)	Complementary strand	4193023	4193658
JW3958(thiC)	Complementary strand	4193658	4195553
JW3959(rsd)	Complementary strand	4195786	4196262
JW3960(yjaD)		4196357	4197130
JW3961(hemE)		4197170	4198234
JW3962(nfi)		4198238	4198915
JW3963(yjaG)		4198958	4199548
JW3964(hupA)		4199735	4200007
JW3965(yjaH)		4200020	4200715
JW3966(yjaI)	Complementary strand	4200717	4201283
JW3967(hydH)		4201380	4202777
JW3968(hydG)		4202774	4204099
JW3969(purD)	Complementary strand	4204096	4205385
JW3970(purH)	Complementary strand	4205397	4206986
JW3971(yjaA)		4212689	4213072
JW3972(yjaB)	Complementary strand	4213135	4213578
JW3973(metA)		4213735	4214664
JW3974(aceB)		4214933	4216534
JW3975(aceA)		4216564	4217868
JW3976(aceK)		4218051	4219787
JW3977(arp)	Complementary strand	4219756	4221942
JW3978(iclR)	Complementary strand	4222259	4223083
JW3979(metH)		4223283	4226966
JW3980(yjbB)		4227186	4228817
JW3981(pepE)	Complementary strand	4228908	4229597
JW3982(yjbC)		4229809	4230681
JW3983(yjbD)	Complementary strand	4230814	4231086
JW3984(lysC)	Complementary strand	4231339	4232688
JW3985(pgi)		4233213	4234862
JW3986(633#6)		4235361	4235603
JW3987(yjbF)		4235687	4236355
JW3988(yjbG)		4236352	4237089
JW3989(yjbH)		4237089	4239185
JW3989.5(633#10)	Complementary strand	4239232	4239510
JW3990(yjbA)		4239780	4240190
JW3991(xylE)	Complementary strand	4240234	4241709
JW3992(malG)	Complementary strand	4242081	4242971
JW3993(malF)	Complementary strand	4242986	4244530
JW3994(malE)	Complementary strand	4244684	4245874
JW3995(malK)		4246239	4247354
JW3996(lamB)		4247426	4248766
JW3997(malM)		4249009	4249929
JW3998(yjbI)		4250410	4251738
JW3999(ubiC)		4251850	4252458
JW4000(ubiA)		4252471	4253343
JW4001(plsB)	Complementary strand	4253498	4255921
JW4002(dgkA)		4256092	4256460
JW4003(lexA)		4256570	4257178
JW4004(dinF)		4257197	4258576
JW4005(yjbJ)		4258692	4258901
JW4006(yjbK)	Complementary strand	4258943	4259518
JW4007(yjbL)		4259776	4260030
JW4008(yjbM)		4260054	4260761
JW4009(yjbN)		4261124	4262161
JW4010(yjbO)		4262085	4262537
JW4011(qor)	Complementary strand	4262703	4263686
JW4012(dnaB)		4263769	4265184
JW4013(alr)		4265237	4266316
JW4014(tyrB)		4266569	4267762
JW4015(napA)		4268869	4269582
JW4016(hobH)	Complementary strand	4269310	4269537
JW4017(yjbQ)		4269693	4270109
JW4018(yjbR)		4270113	4270469
JW4019(uvrA)	Complementary strand	4270504	4273326
JW4020(ssb)		4273580	4274116
JW4021(yjcB)	Complementary strand	4274215	4274565
JW4022(yjcC)		4274926	4276512
JW4023(soxS)	Complementary strand	4276515	4276838
JW4024(soxR)		4276924	4277388
JW4025(yjcD)		4277934	4279283
JW4026(yjcE)		4279435	4281084
JW4027(yjcF)	Complementary strand	4281238	4282530
JW4028(yjcG)	Complementary strand	4282708	4284357
JW4029(yjcH)	Complementary strand	4284354	4284668
JW4030(acs)	Complementary strand	4284868	4286826
JW4031(nrfA)		4287219	4288655
JW4032(nrfB)		4288700	4289266
JW4033(nrfC)		4289263	4289934
JW4034(nrfD)		4289931	4290887
JW4035(nrfE)		4290967	4292625
JW4036(nrfF)		4292618	4293001
JW4037(nrfG)		4292998	4293594
JW4038(gltP)		4293936	4295249
JW4039(yjcO)	Complementary strand	4295891	4296580
JW4040(fdhF)	Complementary strand	4296674	4298821
JW4041(yjcP)	Complementary strand	4299019	4300485
JW4042(yjcQ)	Complementary strand	4300482	4302533
JW4043(yjcR)	Complementary strand	4302533	4303564
JW4044(yjcS)	Complementary strand	4304067	4306064
JW4045(alsK)	Complementary strand	4306325	4307254
JW4046(alsE)	Complementary strand	4307238	4307933
JW4047(alsC)	Complementary strand	4307944	4308924
JW4048(alsA)	Complementary strand	4308903	4310435
JW4049(alsB)	Complementary strand	4310562	4311497
JW4050(alsR)	Complementary strand	4311556	4312446
JW4051(rpiB)		4312805	4313254
JW4052(phnQ)	Complementary strand	4313265	4313672
JW4053(phnP)	Complementary strand	4313798	4314556
JW4054(phnO)	Complementary strand	4314558	4314992
JW4055(phnN)	Complementary strand	4314979	4315536
JW4056(phnM)	Complementary strand	4315536	4316672
JW4057(phnL)	Complementary strand	4316669	4317349
JW4058(phnK)	Complementary strand	4317460	4318218
JW4059(phnJ)	Complementary strand	4318215	4319060
JW4060(phnI)	Complementary strand	4319053	4320117
JW4061(phnH)	Complementary strand	4320117	4320701
JW4062(phnG)	Complementary strand	4320698	4321150
JW4063(phnF)	Complementary strand	4321151	4321876
JW4064(phnE)	Complementary strand	4321897	4322265
JW4065(phnE)	Complementary strand	4322115	4322735
JW4066(phnD)	Complementary strand	4322790	4323806
JW4067(phnC)	Complementary strand	4323831	4324619
JW4068(phnB)	Complementary strand	4324752	4325195
JW4069(phnA)	Complementary strand	4325853	4326188
JW4070(yjdA)		4326589	4328817
JW4071(yjcZ)		4328847	4329692
JW4072(proP)		4329956	4331458
JW4073(basS)	Complementary strand	4331635	4332726
JW4074(basR)	Complementary strand	4332736	4333404
JW4075(yjdB)	Complementary strand	4333401	4335074
JW4076(yjdE)	Complementary strand	4335148	4336485
JW4077(adiY)	Complementary strand	4336622	4337383
JW4078(adi)	Complementary strand	4337708	4339975
JW4079(melR)	Complementary strand	4340174	4341082
JW4080(melA)		4341365	4342720
JW4081(melB)		4342835	4344244
JW4082(yjdF)	Complementary strand	4344383	4345012
JW4083(fumB)	Complementary strand	4345134	4346780
JW4084(dcuB)	Complementary strand	4346858	4348198
JW4085(dcuR)	Complementary strand	4348769	4349488
JW4086(dcuS)	Complementary strand	4349485	4351116
JW4087(yjdI)		4351297	4351527
JW4088(yjdJ)		4351539	4351811
JW4089(yjdK)		4352038	4352334
JW4090(lysU)	Complementary strand	4352654	4354171
JW4091(yjdL)	Complementary strand	4354408	4355865
JW4092(cadA)	Complementary strand	4355924	4358071
JW4093(cadB)	Complementary strand	4358151	4359485
JW4094(cadC)	Complementary strand	4359850	4361388
JW4095(cutA3)	Complementary strand	4362187	4362786
JW4096(cutA2)	Complementary strand	4362799	4364268
JW4097(cutA)	Complementary strand	4364472	4364810
JW4098(dcuA)	Complementary strand	4364926	4366227
JW4099(aspA)	Complementary strand	4366345	4367781
JW4100(fxsA)		4368118	4368594
JW4101(yjeH)	Complementary strand	4368610	4369866
JW4102(groES)		4370142	4370435
JW4103(groEL)		4370479	4372125
JW4104(yjeI)		4372230	4372616
JW4105(yjeJ)	Complementary strand	4372819	4373688
JW4106(yjeK)	Complementary strand	4374083	4375111
JW4107(efp)		4375153	4375719
JW4108(ecnB)		4376007	4376153
JW4109(sugE)		4376179	4376646
JW4110(blc)	Complementary strand	4376643	4377176
JW4111(ampC)	Complementary strand	4377265	4378398
JW4112(frdD)	Complementary strand	4378461	4378820
JW4113(frdC)	Complementary strand	4378831	4379226
JW4114(frdB)	Complementary strand	4379237	4379971
JW4115(frdA)	Complementary strand	4379964	4381772
JW4116(genX)		4382097	4383074
JW4117(yjeM)		4383251	4384795
JW4118(yjeN)		4384847	4385161
JW4119(yjeO)		4385158	4385472
JW4120(yjeP)	Complementary strand	4385501	4388824
JW4121(psd)	Complementary strand	4388846	4389814
JW4122(yjeQ)	Complementary strand	4389911	4390963
JW4123(yjeR)		4390989	4391603
JW4124(yjeS)	Complementary strand	4392382	4393521
JW4125(yjeF)		4393520	4395067
JW4126(yjeE)		4395039	4395500
JW4127(amiB)		4395519	4396856
JW4128(mutL)		4396866	4398713
JW4129(miaA)		4398706	4399656
JW4130(hfq)		4399742	4400050
JW4131(hflX)		4400126	4401406
JW4132(hflK)		4401492	4402751
JW4133(hflC)		4402754	4403758
JW4134(yjeT)		4403840	4404037
JW4135(purA)		4404141	4405439
JW4136(yjeB)		4405644	4406069
JW4137(vacB)		4406066	4408549
JW4138(yjfH)		4408729	4409460
JW4139(yjfI)		4409587	4409988
JW4140(yjfJ)		4410007	4410705
JW4141(yjfK)		4410756	4411415
JW4142(yjfL)		4411433	4411831
JW4143(yjfM)		4411841	4412479
JW4144(yjfC)		4412482	4413645
JW4145(aidB)		4413714	4415354
JW4146(yjfN)	Complementary strand	4415471	4415773
JW4147(yjfO)	Complementary strand	4415895	4416323
JW4148(yjfP)		4416406	4417155
JW4149(yjfQ)	Complementary strand	4417152	4417907
JW4150(yjfR)	Complementary strand	4418015	4419085
JW4151(sgaT)		4419377	4420831
JW4152(sgaB)		4420847	4421152
JW4153(sgaA)		4421162	4421626
JW4154(sgaH)		4421640	4422290
JW4155(sgaU)		4422300	4423154
JW4156(sgaE)		4423154	4423840
JW4157(yjfY)	Complementary strand	4423970	4424245
JW4158(rpsF)		4424572	4424967
JW4159(priB)		4424974	4425288
JW4160(rpsR)		4425293	4425520
JW4161(rplI)		4425562	4426011
JW4162(yjfZ)	Complementary strand	4426082	4426876
JW4163(ytfA)		4427223	4427549
JW4164(ytfB)	Complementary strand	4427533	4428207
JW4165(fklB)		4428230	4429009
JW4166(cycA)		4429318	4430730
JW4167(ytfE)	Complementary strand	4430775	4431437
JW4168(ytfF)	Complementary strand	4431545	4432519
JW4169(ytfG)	Complementary strand	4432618	4433478
JW4170(ytfH)		4433477	4433947
JW4171(cpdB)	Complementary strand	4434076	4436019
JW4172(cysQ)		4436209	4436949
JW4173(ytfI)		4437161	4437661
JW4174.1(657#1.2)		4437652	4438098
JW4175(ytfJ)	Complementary strand	4438161	4438715
JW4176(ytfK)		4439001	4439246
JW4177(ytfL)	Complementary strand	4439325	4440668
JW4178(msrA)	Complementary strand	4440991	4441629
JW4179(ytfM)		4441835	4443568
JW4180(ytfN)		4443565	4447344
JW4181(ytfP)		4447347	4447688
JW4182(yzfA)	Complementary strand	4447429	4447698
JW4183(chpS)		4447894	4448151
JW4184(chpB)		4448145	4448495
JW4185(ppa)	Complementary strand	4448575	4449105
JW4186(ytfQ)		4449415	4450371
JW4187(ytfR)		4450509	4451762
JW4188(ytfS)		4451738	4452010
JW4189(ytfT)		4452021	4453046
JW4190(yjfF)		4453057	4454028
JW4191(fbp)	Complementary strand	4454061	4455059
JW4192(mpl)		4455235	4456608
JW4193(yjgA)	Complementary strand	4456764	4457315
JW4194(pmbA)		4457409	4458761
JW4195(cybC)		4459003	4459305
JW4196(nrdG)	Complementary strand	4459350	4459814
JW4197(nrdD)	Complementary strand	4459972	4462110
JW4198(treC)	Complementary strand	4462504	4464159
JW4199(treB)	Complementary strand	4464209	4465630
JW4200(treR)	Complementary strand	4465749	4466696
JW4201(mgtA)		4467075	4469771
JW4202(yjgF)	Complementary strand	4469977	4470402
JW4203(pyrI)	Complementary strand	4470436	4470897
JW4204(pyrB)	Complementary strand	4470910	4471845
JW4205(pyrBI)	Complementary strand	4471849	4471983
JW4206(yjgH)	Complementary strand	4472264	4472659
JW4207(yjgI)	Complementary strand	4472790	4473503
JW4208(yjgJ)		4473574	4474167
JW4209(yjgK)		4474303	4474764
JW4210(yjgL)		4474851	4476701
JW4211(argI)	Complementary strand	4476746	4477750
JW4212(yjgD)		4477912	4478328
JW4213(yjgM)	Complementary strand	4478473	4478766
JW4214(yjgN)		4479186	4480367
JW4215(valS)	Complementary strand	4480426	4483281
JW4216(holC)	Complementary strand	4483281	4483724
JW4217(xerB)	Complementary strand	4483884	4485395
JW4218(yjgP)		4485662	4486762
JW4219(yjgQ)		4486759	4487844
JW4220(yjgR)	Complementary strand	4488005	4489507
JW4221(idnR)	Complementary strand	4489585	4490583
JW4222(idnT)	Complementary strand	4490650	4491969
JW4223(idnO)	Complementary strand	4492031	4492795
JW4224(idnD)	Complementary strand	4492819	4493850
JW4225(idnK)		4494067	4494630
JW4226(yjgB)	Complementary strand	4494634	4495695
JW4227(intB)		4496194	4497384
JW4228(660#10.1)	Complementary strand	4497565	4497705
JW4229(yi2A)		4497671	4498081
JW4230(yi22)		4498039	4498944
JW4231(yi24)	Complementary strand	4498084	4498515
JW4232(yi23)		4498112	4498405
JW4233(yjgW)		4499043	4499378
JW4234(yjgX)	Complementary strand	4499487	4499933
JW4235(yjgY)	Complementary strand	4499876	4500325
JW4236(yjgZ)		4500704	4501033
JW4237(yi41)	Complementary strand	4501547	4502893
JW4238(yi42)		4502279	4502746
JW4239(yjhB)		4503442	4504719
JW4240(yjhC)		4504716	4505849
JW4241(yjhD)	Complementary strand	4506070	4506444
JW4242(yjhE)		4506305	4506553
JW4243(yi91)		4506605	4506907
JW4244(662#3.2)	Complementary strand	4506910	4508061
JW4245(662#4)		4508402	4508998
JW4246(662#5)		4509164	4509577
JW4247(fecE)	Complementary strand	4510134	4510901
JW4248(fecD)	Complementary strand	4510902	4511858
JW4249(fecC)	Complementary strand	4511855	4512853
JW4250(fecB)	Complementary strand	4512850	4513752
JW4251(fecA)	Complementary strand	4513797	4516121
JW4252(fecR)	Complementary strand	4516208	4517161
JW4253(fecI)	Complementary strand	4517158	4517679
JW4254(insA)		4517971	4518246
JW4255(insB)		4518165	4518458
JW4256(insB)		4518459	4518668
JW4257(yjhU)	Complementary strand	4518782	4519582
JW4258(yjhF)	Complementary strand	4520115	4521464
JW4259(yjhG)	Complementary strand	4521571	4523538
JW4260(yjhH)	Complementary strand	4523549	4524508
JW4261(yjhI)	Complementary strand	4524459	4525247
JW4262(sgcR)	Complementary strand	4525550	4526332
JW4263(sgcE)	Complementary strand	4526349	4526981
JW4264(sgcA)	Complementary strand	4526993	4527424
JW4265(sgcQ)	Complementary strand	4527555	4528361
JW4266(sgcC)	Complementary strand	4528374	4529687
JW4266.5(664#6)	Complementary strand	4529699	4529977
JW4267(sgcX)	Complementary strand	4529974	4531125
JW4268(yjhP)	Complementary strand	4531881	4532627
JW4269(yjhQ)	Complementary strand	4532683	4533228
JW4269.5(664#11)	Complementary strand	4533240	4533497
JW4270(664#12)	Complementary strand	4533988	4534119
JW4270.5(664#13)		4534283	4534492
JW4271(yjhR)		4534459	4535475
JW4272(yjhS)	Complementary strand	4536058	4537038
JW4273(yjhT)	Complementary strand	4537103	4538317
JW4274(yjhA)	Complementary strand	4538229	4538954
JW4275(fimB)		4540401	4541003
JW4276(fimE)		4541481	4542077
JW4277(fimA)		4542559	4543107
JW4278(fimI)		4543064	4543711
JW4279(fimC)		4543748	4544473
JW4280(fimD)		4544541	4547177
JW4281(fimF)		4547187	4547717
JW4282(fimG)		4547730	4548233
JW4283(fimH)		4548253	4549155
JW4284(gntP)	Complementary strand	4549398	4550741
JW4285(uxuA)		4551081	4552265
JW4286(uxuB)		4552346	4553806
JW4287(uxuR)		4554021	4554794
JW4288(yjiC)	Complementary strand	4554935	4555765
JW4289(yjiD)		4556429	4556830
JW4290(yjiE)	Complementary strand	4556823	4557734
JW4291(iadA)	Complementary strand	4557799	4558971
JW4292(yjiG)	Complementary strand	4558984	4559445
JW4293(yjiH)	Complementary strand	4559442	4560137
JW4294(yjiI)		4560273	4560929
JW4295(yjiJ)	Complementary strand	4560942	4562120
JW4296(yjiK)	Complementary strand	4562188	4563159
JW4296.5(668#5)	Complementary strand	4563113	4563370
JW4297(yjiL)	Complementary strand	4563367	4564140
JW4298(yjiM)	Complementary strand	4564144	4565316
JW4299(yjiN)	Complementary strand	4565411	4566691
JW4300(yjiO)	Complementary strand	4566732	4567964
JW4301(yjiP)		4568443	4568754
JW4302(yjiQ)		4568803	4569363
JW4303(yjiR)	Complementary strand	4569607	4571019
JW4304(yjiS)		4571196	4571360
JW4305(yjiT)		4571811	4573376
JW4306(yjiV)		4573580	4575121
JW4307(mcrD)		4575072	4576301
JW4308(mcrC)	Complementary strand	4576358	4577434
JW4309(mcrB)	Complementary strand	4577404	4578801
JW4310(yjiW)	Complementary strand	4578945	4579343
JW4311(hsdS)	Complementary strand	4579514	4580908
JW4312(hsdM)	Complementary strand	4580905	4582494
JW4313(hsdR)	Complementary strand	4582695	4586261
JW4314(mrr)		4586395	4587309
JW4315(yjiA)	Complementary strand	4587355	4588209
JW4316(yjiX)	Complementary strand	4588322	4588525
JW4317(yjiY)	Complementary strand	4588575	4590740
JW4318(tsr)		4591103	4592758
JW4319(yjjL)	Complementary strand	4592807	4594168
JW4320(671#8)		4592916	4593062
JW4321(yjjM)	Complementary strand	4594383	4595189
JW4322(yjjN)		4595420	4596457
JW4323(mdoB)	Complementary strand	4596595	4598847
JW4324(yjjA)	Complementary strand	4599140	4599637
JW4325(dnaC)	Complementary strand	4599683	4600420
JW4326(dnaT)	Complementary strand	4600423	4600962
JW4327(yjjB)	Complementary strand	4601069	4601395
JW4328(yjjP)	Complementary strand	4601533	4602366
JW4329(yjjQ)		4602922	4603647
JW4330(bglJ)		4603605	4604282
JW4331(fhuF)	Complementary strand	4604320	4605108
JW4332(yjjZ)		4605210	4605485
JW4333(rsmC)	Complementary strand	4606114	4607145
JW4334(holD)		4607248	4607661
JW4335(rimI)		4607630	4608076
JW4336(yjjG)		4608091	4608768
JW4337(prfC)		4608859	4610448
JW4338(osmY)		4610841	4611446
JW4339(674#2)		4611555	4611734
JW4340(yjjU)		4611856	4612929
JW4341(yjjV)		4612926	4613705
JW4342(yjjW)	Complementary strand	4614125	4614988
JW4343(yjjI)	Complementary strand	4614960	4616510
JW4344(deoC)		4616768	4617547
JW4345(deoA)		4617674	4618996
JW4346(deoB)		4619048	4620271
JW4347(deoD)		4620328	4621047
JW4348(yjjJ)		4621214	4622545
JW4349(lplA)	Complementary strand	4622546	4623562
JW4350(smp)	Complementary strand	4623590	4624234
JW4351(serB)		4624340	4625308
JW4352(radA)		4625357	4626739
JW4353(nadR)		4626739	4627992
JW4354(yjjK)	Complementary strand	4628300	4629967
JW4355(slt)		4630178	4632115
JW4356(trpR)		4632205	4632531
JW4357(yjjX)	Complementary strand	4632678	4633199
JW4358(gpmG)		4633242	4633889
JW4359(rob)	Complementary strand	4633886	4634755
JW4360(creA)		4634966	4635439
JW4361(creB)		4635452	4636141
JW4362(creC)		4636141	4637565
JW4363(creD)		4637623	4638975
JW4364(arcA)	Complementary strand	4639035	4639751
JW4365(yjjY)		4639847	4639987
JW4366(lasT)		4640387	4641073
JW4367(thrL)		4641287	4641352

Further, the E. coli mutant strain of the present invention includes; (43) The E. coli mutant strain according to any one of (1) to (42) obtained via modification by a method selected from among the following [1] to [4]:

• [1] a method for increasing the expression level of at least one enzyme associated with biosynthesis of the useful substance;
• [2] a method for increasing the copy number of at least one enzyme gene associated with biosynthesis of the useful substance;
• [3] a method for attenuating or blocking at least one metabolic pathway which branches from the biosynthetic pathway of the useful substance into metabolites other than the useful substance; or
• [4] a method for selecting a strain that exhibits higher resistance to an analogue of a useful substance than a wild-type E. coli strain.

The E. coli mutant strains described in (1) to (42) above appear to lack parts of or entire genes that encode proteins that are not directly involved in biosynthesis of useful substances but indirectly suppress generation of such useful substances. Such mutant strains and the mutant strain (43) are useful for producing useful substances.

The E. coli mutant strain of the present invention may be any E. coli mutant strain, provided that it belongs to Escherichia coli. It is preferably a mutant strain of the E. coli K-12 strain, the E. coli B strain, or the E. coli W strain, more preferably a mutant strain of the E. coli K-12 strain, further preferably a mutant strain of the E. coli W3110 strain (ATCC27325) or the E. coli MG1655 strain (ATCC47076), and particularly preferably a mutant strain of the E. coli W3110 strain (ATCC27325), for example.

2. Method for Producing the E. coli Mutant Strain of the Present Invention

The E. coli mutant strain of the present invention can be obtained by any method, provided that it allows such mutant strain to be obtained. For example, the following method wherein a given gene or DNA on E. coli chromosomal DNA is deleted may be employed.

A gene or DNA of E. coli chromosomal DNA can be deleted via a method utilizing homologous recombination, for example. An example of a method utilizing general homologous recombination is a method involving the use of plasmids for homologous recombination that can be prepared by ligating DNA fragments located at both outer sides of the gene or DNA that is intended to be deleted on E. coli chromosomal DNA to plasmid DNA having a drug-resistant gene that cannot be autonomously replicated in such E. coli.

After the plasmids for homologous recombination are introduced into a host cell by a conventional method, transformants into which the plasmids for homologous recombination have been integrated to chromosomal DNA by homologous recombination are selected using the drug resistance as an indicator. The obtained transformants are cultured in a medium that does not contain the aforementioned drug for several hours to 1 day, the cultures are applied to an agar medium that contains the drug and to an agar medium that does not contain the drug, and strains that do not grow in the former medium but grow in the latter medium are selected. Thus, strains in which the second homologous recombination has taken place on the chromosomal DNA can be obtained. By determining the nucleotide sequence of a region of the chromosomal DNA in which the gene or DNA to be deleted was present, the introduction of deletion of the target gene or DNA on chromosomal DNA can be confirmed.

An example of a method utilizing homologous recombination, and thereby effectively introducing deletion, substitution or addition of nucleotide(s) into multiple genes, is a method involving the use of a linear DNA.

Specifically, a linear DNA that contains DNA fragments existing at both outside regions of a gene or DNA to be deleted on chromosomal DNA is corporated into a cell to cause homologous recombination between the chromosomal DNA and the introduced linear DNA. This method can be applied to any microorganisms, provided that such Escherichia coli effectively incorporate a linear DNA. Such microorganisms are preferably Escherichia coli that expresses λ phage-derived recombinant proteins (Red recombination system).

An example of Escherichia coli that expresses the λRed recombination system is Escherichia coli JM101 comprising pKD46, which is plasmid DNA having the gene of the λ Red recombination system (available from the E. coli Genetic Stock Center, Yale University, U.S.A.).

Examples of DNAs that can be used for homologous recombination include:

• (a) a linear DNA comprising, at both ends of the drug-resistant gene, DNA existing at both outside regions of a chromosomal DNA into which the introduction of deletion, substitution or addition of nucleotide(s) is intended, or DNA homologous thereto;
• (b) a linear DNA to which DNA existing at both outside regions of a chromosomal DNA into which the introduction of deletion, substitution or addition of nucleotide(s) is intended, or DNA homologous thereto is directly ligated;
• (c) a linear DNA comprising, at both ends of DNA to which a drug-resistant gene and a gene that can be used for negative selection are ligated, DNA existing at both outside regions of chromosomal DNA into which the introduction of deletion, substitution or addition of nucleotide(s) is intended, or DNA homologous thereto; and
• (d) a linear DNA according to (a), which further comprises a nucleotide sequence which is recognized by yeast-derived Flp recombinase [Proc. Natl. Acad. Sci., U.S.A., 82, 5875, 1985] between a drug-resistant gene and DNA existing at both outside regions of a chromosomal DNA or DNA homologous thereto.

Any drug-resistant genes can be used, provided that such genes impart drug resistance to a drug to which E. coli shows sensitivity. For example, kanamycin-resistant genes, chloramphenicol-resistant genes, gentamicin-resistant genes, spectinomycin-resistant genes, tetracycline-resistant genes, or ampicillin-resistant genes can be used as drug-resistant genes.

The genes that can be used for negative selection are genes that are lethal to E. coli under given culture conditions, when such genes are expressed in E. coli. Examples of such genes include sacB genes derived from the microorganism which belongs to the genus Bacillus [Appl. Environ. Microbiol., 59, 1361-1366, 1993] and rpsL genes derived from the microorganism which belongs to the genus Escherichia [Genomics, 72, 99-104, 2001].

DNA that exists at both ends of the aforementioned linear DNA which is located at both outside regions of a chromosomal DNA into which introduction of substitution or deletion of nucleotide(s) is intended, or DNA homologous thereto, is oriented on the linear DNA in the same direction as the direction thereof on the chromosomal DNA. The length thereof is preferably about 10 bp to 100 bp, more preferably about 20 bp to 50 bp, and further preferably about 30 bp to 40 bp.

The nucleotide sequence that is recognized by yeast-derived Flp recombinase may be a nucleotide sequence that is recognized by yeast-derived Flp recombinase and has a nucleotide sequence which catalyze homologous recombination, provided that the aforementioned protein recognizes such nucleotide sequence and catalyzes homologous recombination of such nucleotide sequence.

“A DNA homologous thereto” refers to a DNA which has identity to the extent that homologous recombination takes place between the aforementioned linear DNA and a target region on a chromosomal DNA. Specifically, it refers to 80% or more, preferably 90% or more, more preferably 95% or more, and further preferably 100% homology.

Homology of the nucleotide sequences can be determined using the aforementioned programs such as BLAST or FASTA.

The aforementioned linear DNA can be prepared by PCR. Also, DNA containing the linear DNA can be constructed on the plasmid and the linear DNA of interest can then be obtained by restriction enzyme treatment.

Deletion, substitution, or addition of nucleotide(s) can be introduced into chromosomal DNAs of E. coli by any of the following methods 1 to 4:

• Method 1: a method wherein the linear DNA (a) or (d) is introduced into a host microorganism, and a transformant, into which such linear DNA has been integrated into a chromosomal DNA by homologous recombination, is selected using drug resistance as an indicator;

Method 2: a method wherein a DNA to which DNA existing at both outside regions of a chromosomal DNA into which the introduction of deletion, substitution or addition of nucleotide(s) is intended, or DNA homologous thereto is directly ligated, is introduced into the transformant obtained by the above method 1, and the drug-resistant gene integrated into chromosomal DNA by this method is deleted so as to substitute or delete a region on a chromosomal DNA of a microorganism;

Method 3:

• [1] introducing the linear DNA (c) into a host microorganism and selecting a transformant into which the linear DNA has been integrated into chromosomal DNA by homologous recombination using drug resistance as an indicator,
• [2] synthesizing a DNA obtained by ligating DNAs homologous to DNAs located at both outside regions of a target region of substitution or deletion on a chromosomal DNA in the same direction on the chromosomal DNA and introducing the synthesized product into the transformant obtained in [1] above, and
• [3] culturing transformants subjected to the procedure [2] under conditions where the genes that can be used for negative selection are expressed, and selecting strains that can grow in the culture condition as strains in which the drug-resistant genes and genes that can be used for negative selection have been deleted from the chromosomal DNA; and Method 4:
• [1] introducing the linear DNA (d) into a host microorganism and selecting a transformant into which the linear DNA has been integrated into chromosomal DNA by homologous recombination using drug resistance as an indicator, and
• [2] introducing Flp recombinase gene-expressing plasmid into the transformant obtained in the above [1] to express the Flp recombinase gene, and obtaining strains sensitive to the drug used in the above[1].

A method of introducing a linear DNA into a host microorganism that is employed in the above methods can be any method as long as the DNA can be introduced into the microorganism. Examples thereof include a method involving the use of calcium ions [Proc. Natl. Acad. Sci., U.S.A., 69, 2110, 1972], the protoplast method [Japanese Published Unexamined Patent Application No. 2483942/88(1988)], and electroporation [Nucleic Acids Res., 16, 6127, 1988].

By using the DNA comprising at a site around the center thereof any gene to be inserted into a chromosomal DNA as the DNA that is used in method 2 or method 3 [2], drug-resistant genes or the like can be deleted, and any genes can be simultaneously introduced into the chromosomal DNA.

According to the methods 2 to 4, foreign genes, such as drug-resistant genes and genes that can be used for negative selection, do not remain in the chromosomal DNA of the transformant which is finally obtained. With the utilization of such methods, accordingly, the same drug-resistant genes and genes that can be used for negative selection may be used, and the procedures of such methods are repeated. This enables the easy production of E. coli having deletion of gene or DNA at two or more different regions on a chromosomal DNA.

The amounts of the E. coli mutant strains obtained by such method are greater than those of wild-type E. coli strains after a given period of culture. This can be confirmed by, for example, culturing the wild-type parental E. coli strains and the mutant strains using M9 minimal medium that contains 1% glucose, 0.6% disodium phosphate, 0.3% potassium dihydrogenphosphate, 0.05% sodium chloride, 0.1% ammonium chloride, 2 mmol/l of magnesium sulfate heptahydrate, 10 mg/l of iron sulfate and 100 μmol/l of calcium chloride, or LB liquid medium containing 3% glucose (10 g/l of Bacto tryptone (Difco), 5 g/l of Bacto yeast extract (Difco), 5 g/l of sodium chloride, and 1 ml/l of 1 mol/l sodium hydroxide), and assaying absorption of culture products at 660 nm or 590 nm at a given frequency, or assaying an ATP content per unit of medium via a conventional technique.

Also, the E. coli mutant strain of the present invention can be obtained by a method wherein a gene or DNA of chromosomal DNA of E. coli capable of producing a useful substance is deleted or a method wherein the capacity for producing a useful substance is imparted to E. coli from which a gene or DNA of chromosomal DNA has been deleted.

E. coli capable of producing a useful substance may be any E. coli, provided that it is capable of producing one or more types of useful substances. Examples of such E. coli include a strain isolated from nature that is capable of producing a useful substance, and E. coli to which the ability to produce a desirable useful substance has been artificially imparted by conventional methods.

Examples of such conventional methods include:

• (a) a method for reducing or removing at least one mechanism for regulating biosynthesis of a useful substance;
• (b) a method for increasing the expression level of at least one enzyme associated with biosynthesis of a useful substance;
• (c) a method for increasing the copy number of at least one enzyme gene associated with biosynthesis of a useful substance;
• (d) a method for attenuating or blocking at least one metabolic pathway which branches from a biosynthetic pathway of a useful substance into metabolites other than the useful substance; and
• (e) a method for selecting a strain that exhibits higher resistance to an analogue of a useful substance than a wild-type strain.
• Such conventional methods can be carried out alone or in combinations of two or more.

When useful substances are amino acids, for example, method (a) is described in Agric. Biol. Chem., 43, 105-111, 1979, J. Bacteriol., 110, 761-763, 1972, and Appl. Microbiol. Biotechnol., 39, 318-323, 1993; method (b) is described in Agric. Biol. Chem., 43, 105-111, 1979 and J. Bacteriol., 110, 761-763, 1972; method (c) is described in Appl. Microbiol. Biotechnol., 39, 318-323, 1993 and Agric. Biol. Chem., 39, 371-377, 1987; method (d) is described in Appl. Environ. Microbiol., 38, 181-190, 1979 and Agric. Biol. Chem., 42, 1773-1778, 1978; and method (e) is described in Agric. Biol. Chem., 36, 1675-1684, 1972, Agric. Biol. Chem., 41, 109-116, 1977, Agric. Biol. Chem., 37, 2013-2023, 1973, and Agric. Biol. Chem., 51, 2089-2094, 1987. With reference to these literatures, microorganisms capable of forming and accumulating various amino acids can be produced.

Also, many examples of a method for producing microorganisms capable of forming and accumulating amino acids by any of or two or more of methods (a) to (e) above are described in Biotechnology 2nd ed., vol. 6, Products of Primary Metabolism (VCH Verlagsgesellschaft mbH, Weinheim, 1996), section 14a or 14b, Advances in Biochemical Engineering/Biotechnology 79, 1-35, 2003, or Amino san Hakko (Amino acid fermentation), Japan Scientific Societies Press, Hiroshi Aida et al. (1986). In addition, many reports have been made concerning a specific method for producing microorganisms capable of forming and accumulating amino acids. Examples thereof include Japanese Published Unexamined Patent Application No. 2003-164297, Agric. Biol. Chem., 39, 153-160, 1975, Agric. Biol. Chem., 39, 1149-1153, 1975, Japanese Published Unexamined Patent Application No. 13599/83(1983), J. Gen. Appl. Microbiol., 4, 272-283, 1958, Japanese Published Unexamined Patent Application No. 94985/88(1988), Agric. Biol. Chem., 37, 2013-2023, 1973, WO 97/15673, Japanese Published Unexamined Patent Application No. 18596/81(1981), Japanese Published Unexamined Patent Application No. 144092/81(1981), and Japanese Published Unexamined Patent Application No. 2003-511086. With reference to these literatures, microorganisms capable of producing one or more kinds of amino acids can be produced. Also, E. coli capable of producing other useful substance can be produced by increase of biosynthetase of said useful substance, and improvement of biosynthesis reguratory mechanism, elimination mechanism and the like in the same way as in the production of E. coli mutant strain capable of producing an amino acid.

3. Method for Producing Useful Substances of the Present Invention

The present invention relates to a method for producing useful substances comprising culturing the E. coli mutant strain of the present invention in a medium so as to generate and accumulate such useful substances in the culture and recovering the useful substances from the culture.

Useful substances are not particularly limited, provided that such substances can be produced with the use of the E. coli mutant strain of the present invention. Examples thereof include proteins, peptides, amino acids, nucleic acids, vitamins, saccharides, organic acids, and lipids. Examples of proteins include inosine kinase, glutamate 5-kinase (EC 2.7.2.11), glutamate-5-semialdehyde dehydrogenase (EC 1.2.1.41), pyrroline-5-carboxylate reductase (EC 1.5.1.2), and human granulocyte-colony-stimulating factors. An example of peptide is glutathione. Examples of amino acids include L-alanine, glycine, L-glutamine, L-glutamic acid, L-asparagine, L-aspartic acid, L-lysine, L-methionine, L-threonine, L-leucine, L-valine, L-isoleucine, L-proline, L-histidine, L-arginine, L-tyrosine, L-tryptophane, L-phenylalanine, L-serine, L-cysteine, L-3-hydroxyproline, and L-4-hydroxyproline. Examples of nucleic acids include inosine, guanosine, inosinic acid, and guanylic acid. Examples of vitamins include riboflavin, thiamine, and ascorbic acid. Examples of saccharides include xylose and xylitol. Examples of lipids include eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA).

The E. coli mutant strain that is used for the production method of the present invention can be cultured in accordance with a conventional technique as described below.

A medium for culturing E. coli mutant strain which is used for the production method of the present invention may be natural or synthetic medium as long as it contains carbon sources, nitrogen sources, inorganic salts, etc. assimilable by the microorganisms and the microorganisms can be efficiently cultured therein.

Any carbon sources assimilable by the microorganisms can be used. Examples thereof include: carbohydrates such as glucose, fructose, sucrose, molasses containing any of such substances, starch, and starch hydrolysate; organic acids such as acetic acid and propionic acid; and alcohols such as ethanol and propanol.

Examples of nitrogen sources include: ammonia; ammonium salts of inorganic or organic acids such as ammonium chloride, ammonium sulfate, ammonium acetate, and ammonium phosphate; peptone; meat extract; yeast extract; corn steep liquor; casein hydrolysate; soybean cake and hydrolysate of soybean cake; and various fermentation microorganisms and digests thereof.

Examples of inorganic salts include: monopotassium phosphate, dipotassium phosphate, magnesium phosphate, magnesium sulfate, sodium chloride, iron(I) sulfate, manganese sulfate, copper sulfate, and calcium carbonate.

Culturing is carried out under aerobic conditions such as shaking culture or submerged aeration agitation culture. Culturing temperature is preferably 15 to 40° C., and culture time is generally 16 hours to 7 days. During culturing, the pH is preferably maintained at 3.0 to 9.0. The pH is adjusted with an inorganic or organic acid, an alkali solution, urea, calcium carbonate, ammonia, or the like.

When useful substances are generated and accumulated extracellularly, precipitates such as cells are removed from the culture after the completion of culturing, and target useful substances can be isolated and purified from the culture via ion-exchange treatment, concentration, salting out, or other means. Such techniques can be employed in combination.

When useful substances are generated and accumulated intracellularly, cells are recovered from the culture after the completion of culturing and then disrupted via adequate means such as mechanical or chemical means. Target useful substances can be isolated and purified from the solution of disrupted cells via ion-exchange treatment, concentration, salting out, or other means, and such techniques can be employed in combination.

EXAMPLE 1

Construction of Mutant Library of E. coli that Partially lacks Chromosomal DNA

(1) Preparation of E. coli Capable of Homologous Recombination with Linear DNA on Chromosomal DNA

The P1 phages were prepared from E. coli KM22 strains (Gene, 246, 321-330, 2000) capable of homologous recombination with linear DNA on chromosomal DNA in the following manner.

The E. coli KM 22 strains were cultured in LB liquid medium containing 20 mg/l of kanamycin (10 g/l of Bacto tryptone (Difco), 5 g/l of Bacto yeast extract (Difco), 5 g/l of sodium chloride, and 1 ml/l of 1 mol/l sodium hydroxide) at 30° C. overnight. Thereafter, 0.5 ml of culture solution, 1.4 ml of fresh LB liquid medium, and 100 μl of 0.1 mol/l calcium chloride solution were mixed.

The resulting mixture was subjected to shaking culture at 30° C. for 5 to 6 hours, and 400 μl of the mixture was transferred to a sterilized tube. A P1 phage stock solution (2 μl) was added thereto, the mixture was incubated at 37° C. for 10 minutes, 3 ml of a soft agar solution maintained at 50° C. (10 g/l of Bacto tryptone, 5 g/l of Bacto yeast extract, 5 mmol/l of calcium chloride, 1 ml/l of 1 mol/l sodium hydroxide, and 3 g/l of agar) was added thereto, the resultant was thoroughly agitated, and the resultant was then applied onto a Ca-LB agar medium plate (10 g/l of Bacto tryptone, 5 g/l of Bacto yeast extract, 5 mmol/l of calcium chloride, 1 ml/l of 1 mol/l sodium hydroxide, and 15 g/l of agar; plate diameter: 15 cm).

The plate was subjected to culture at 37° C. for 7 hours, the soft agar portion was finely ground using a spreader and recovered, and the resultant was centrifuged at 1,500 g at 4° C. for 10 minutes. Chloroform (100 μl) was added to 1.5 ml of the supernatant, and the mixture was stored at 4° C. overnight. On the following day, the solution was centrifuged at 15,000 g for 2 minutes, and the supernatant was stored at 4° C. as a phage stock.

Subsequently, the obtained phage was used to transform the E. coli W3110 strain. The culture solution (200 μl) of the E. coli W3 110 strains that had been cultured in Ca-LB liquid medium at 30° C. for 4 hours was fractionated, and 1 μl of phage stock was added. The mixture was incubated at 37° C. for 10 minutes, 5 ml of LB liquid medium and 200 μl of 1 mol/l sodium citrate solution were added, and the mixture was agitated. The resultant was centrifuged at 1,500 g at 25° C. for 10 minutes, the supernatant was discarded, 1 ml of LB liquid medium and 10 μl of 1 mol/l sodium citrate solution were added to the precipitated cells, and the resultant was incubated at 30° C. for 2 hours. The solution (100 μl) was applied to an agar plate medium, Antibiotic Medium 3, containing 20 mg/l of kanamycin, and the plate was subjected to culture at 30° C. overnight. Several colonies that had appeared were applied to an agar plate medium, Antibiotic Medium 3, containing 20 mg/l kanamycin (0.15% meat extract, 0.15% yeast extract, 0.5% peptone, 0.1% glucose, 0.35% sodium chloride, 0.132% dibasic potassium phosphate, and 1.5% agar; pH 7), the drug sensitivity thereof was inspected, and kanamycin-resistant strains were selected. Thus, E. coli W3110red strains into which a chromosomal DNA region (Δ(recC ptr recB recD)::Plac-red kan) capable of homologous recombination with linear DNA on chromosomal DNA had been transduced were obtained.

(2) Construction of Mutant Library of E. coli that Lacks Part of Chromosomal DNA

(i) Preparation of Competent Cells

The E. coli W3110red strains obtained in (1) above were cultured at 30° C. until the OD at 660 nm reached 0.1 using 100 ml of LB liquid medium comprising 1 mmol/l of isopropyl-thio-β-D-galactopyranoside (IPTG). The following procedure was carried out at 4° C. or with the use of an ice-cooled solution.

The obtained culture was centrifuged, and the precipitated cells were suspended in 50 ml of a 20% glycerol solution. The glycerol solution was centrifuged, the precipitated cells were resuspended in a 20% glycerol solution, such procedure was repeated 3 or more times to wash the cells, and the cells were then suspended in 50 μl of a 10% glycerol solution. Thus, a solution of competent cells for electroporation was obtained.

(ii) Preparation of DNA Fragment used for Introducing Deletions into Chromosomal DNA

A DNA fragment used for introducing deletions of about 46 kbp DNA having a nucleotide sequence comprising nucleotides 4489585 to 4535472 of the nucleotide sequence as shown in SEQ ID NO: 1 into chromosomal DNA was produced in the following manner.

DNA having the nucleotide sequence as shown in SEQ ID NO: 2 (DNA comprising a nucleotide sequence comprising nucleotides 4487585 to 4487605 of the nucleotide sequence as shown in SEQ ID NO: 1; hereafter referred to as “primer A”), DNA having the nucleotide sequence as shown in SEQ ID NO: 3 (DNA comprising a nucleotide sequence comprising at the 3′ side of a 25-mer linker sequence a nucleotide sequence comprising nucleotides 4489584 to 4489561 of the nucleotide sequence as shown in SEQ ID NO:1; hereafter referred to as “primer B”), DNA having the nucleotide sequence as shown in SEQ ID NO: 4 (DNA comprising a nucleotide sequence comprising nucleotides 4537475 to 4537451 of the nucleotide sequence as shown in SEQ ID NO: 1; hereafter referred to as “primer C”), and DNA having the nucleotide sequence as shown in SEQ ID NO: 5 (DNA comprising a nucleotide sequence comprising at the 3′ side of a 22-mer linker sequence a nucleotide sequence comprising nucleotides 4535473 to 4535496 of the nucleotide sequence as shown in SEQ ID NO: 1; hereafter referred to as “primer D”) were synthesized.

PCR was carried out using, as a template, chromosomal DNA of the E. coli W3110red strain and, as primer sets, a pair of primer A and primer B and a pair of primer C and primer D. PCR was carried out using LA-Taq (Takara Bio Inc.), and a reaction solution was prepared in accordance with the instructions included therein. Purified chromosomal DNA (100 ng) or small amounts of E. coli W3110red strains recovered with the use of a tip of a pick and 20 pmol of each primer DNA were added to the reaction solution, and PCR was then carried out. PCR was carried out by incubating at 94° C. for 3 minutes, repeating a cycle of 94° C. for 15 seconds, 55° C. for 20 seconds, and 68° C. for 1 minute and 30 seconds 30 times, and then incubating at 72° C. for 10 minutes.

The PCR-amplified DNA fragments were purified using a PCR Purification Kit (Qiagen) and dissolved in 50 μl of water. The DNA fragments were confirmed to be 1-kb DNA fragments homologous to nucleotide sequences at both ends of the target regions for deletion on chromosomal DNA. Hereafter, the DNA fragment amplified with the use of the pair of primer A and primer B is referred to as an upstream arm, and the DNA fragment amplified with the use of the pair of primer C and primer D is referred to as a downstream arm.

Subsequently, PCR was carried out using, as a template, chromosomal DNA of the Bacillus subtilis 168 strain (ATCC23857) and, as a primer set, DNA having the nucleotide sequence as shown in SEQ ID NO: 6 and DNA having the nucleotide sequence as shown in SEQ ID NO: 7. PCR was carried out with the use of a reaction solution of the same composition as above under the same reaction conditions, except for the use of template DNA and primer DNA of different types.

The PCR-amplified DNA fragments were purified in the same manner as described above and then dissolved in 50 Al of water. Such DNA fragments were confirmed to be sacB genes (Genbank accession no. X02730) derived from Bacillus subtilis that can be used for negative selection.

Subsequently, PCR was carried out using, as a template, a pHSG399 plasmid DNA (Takara Bio Inc.) and, as a primer set, DNA having the nucleotide sequence as shown in SEQ ID NO: 8 and DNA having the nucleotide sequence as shown in SEQ ID NO: 9. PCR was carried out with the use of a reaction solution of the same composition as above under the same reaction conditions, except for the use of template DNA and primer DNA of different types.

The PCR-amplified DNA fragments were separated by agarose gel electrophoresis, purified using the QIA quick Gel Extraction Kit (Qiagen), and dissolved in 50 μl of water. The DNA fragments were confirmed to comprise chloramphenicol-resistant genes (cat genes).

Subsequently, PCR was carried out using as templates the DNA fragment comprising the sacB gene and the DNA fragment comprising the cat gene obtained above and, as a primer set, DNA having the nucleotide sequence as shown in SEQ ID NO: 10 and DNA having the nucleotide sequence as shown in SEQ ID NO: 11. PCR was carried out with the use of a reaction solution of the same composition as above under the same reaction conditions, except for the use of template DNA and primer DNA of different types.

The PCR-amplified DNA fragments were separated by agarose gel electrophoresis, purified using the QIA quick Gel Extraction Kit, and dissolved in 50 μl of water. The DNA fragments were confirmed to comprise the sacB gene and the cat gene ligated in the same direction.

Subsequently, PCR was carried out using, as template DNA, a mixture of 3 DNA fragments, i.e., the upstream arm, the downstream arm, and the DNA fragment comprising the sacB gene and the cat gene ligated thereto obtained above and, as a primer set, DNA having the nucleotide sequence as shown in SEQ ID NO: 12 (DNA comprising a nucleotide sequence comprising nucleotides 4488585 to 4488610 of the nucleotide sequence as shown in SEQ ID NO: 1) and DNA having the nucleotide sequence as shown in SEQ ID NO: 13 (DNA comprising a nucleotide sequence comprising nucleotides 4536475 to 4536451 of the nucleotide sequence as shown in SEQ ID NO: 1). PCR was carried out with the use of a reaction solution of the same composition as above under the same reaction conditions, except for the use of, as template DNA, 1 μl of a solution of upstream arm DNA, 1 μl of a solution of downstream arm DNA, and 1.5 μl of a DNA fragment comprising the sacB gene and the cat gene ligated thereto and, as a primer set, DNA having the nucleotide sequence as shown in SEQ ID NO: 12 and DNA having the nucleotide sequence as shown in SEQ ID NO: 13.

The PCR-amplified DNA fragments were separated by agarose gel electrophoresis, purified using the QIA quick Gel Extraction Kit, and dissolved in 50 μl of water. The DNA fragments were confirmed to comprise DNA fragments homologous to both ends of the target region of deletion added to both ends of DNA to which the sacB gene and the cat gene have been ligated (hereafter referred to as a “DNA fragment for regional deletion”).

(iii) Preparation of Mutant Strain of E. coli that Lacks Part of Chromosomal DNA

The DNA fragment for regional deletion obtained above (0.1 μg) was added to 50 μl of a solution of E. coli 3110red competent cells obtained above, and electroporation was carried out using 0.1 cm cuvette (BioRad) at 1.8 kV and 25 μF. The transformants wherein the DNA fragment for regional deletion incorporated into the chromosomes of the E. coli W3110red strain were selected on an LBcm plate (10 g/l of Bacto tryptone (Difco), 5 g/l of Bacto yeast extract (Difco), 20 mg/l of kanamycin, 15 mg/l of chloramphenicol, and 1 ml/l of 1 mol/l NaOH). The colonies of the selected transformants were replicated on an suLB agar medium plate (10 g/l of Bacto tryptone (Difco), 5 g/l of Bacto yeast extract (Difco), 10% of sucrose, 20 mg/l of kanamycin, 15 mg/l of chloramphenicol, and 1 ml/l of 1 moill NaOH) to select strains exhibiting sucrose sensitivity.

A nucleotide sequence comprising nucleotides 4489585 to 4535472 of the nucleotide sequence as shown in SEQ ID NO: 1, which is a target region for deletion on chromosomal DNA of the selected strain, had been substituted with DNA comprising the sacB gene and the cat gene ligated thereto. This substitution was confirmed in the following manner.

PCR was carried out using, as a primer set, DNA comprising the nucleotide sequence as shown in SEQ ID NO: 2 (primer A), which has homologous nucleotide sequences in regions 1 kb upstream and downstream of the ends of the arm sequence of linear DNA used for transformation and DNA comprising the nucleotide sequence as shown in SEQ ID NO: 4 (primer C) and, as a template, chromosomal DNA of the strain selected above. PCR was carried out with the use of a reaction solution of the same composition as above under the same reaction conditions, except for the use of, as a template, small amounts of cell strains selected above (collected from the colonies with the tip of a pick) and, as a primer set, DNA having the nucleotide sequence as shown in SEQ ID NO: 3 and DNA having the nucleotide sequence as shown in SEQ ID NO: 5.

Through the above PCR procedure, a DNA fragment of about 6.7 kb was amplified. A DNA fragment prepared by adding DNA of about 2 kb to both ends of a DNA fragment (2.7 kb) comprising the sacB gene and the cat gene ligated thereto is of about 6.7 kb. Accordingly, a transformant, which was an amplified fragment of about 6.7 kb, obtained via PCR involving the use of a primer set comprising primer A and primer C, was the transformant of interest. Thus, such transformant was designated as the ΔE_suc strain (a deleted region of 4489585 to 4535472 bp).

(iv) Preparation of Mutant Strain of E. coli that Lacks a Partial Region of Chromosomal DNA

Mutants that lack various regions of chromosomal DNAs of E. coli W3110red strains were prepared in the same manner used in the case of preparing the ΔE_suc strains.

A DNA fragment comprising the cat gene and the sucB gene ligated thereto was eliminated from the ΔE_suc strain to prepare an E. coli mutant strain in the following manner. DNA comprising the nucleotide sequence as shown in SEQ ID NO: 14 (DNA comprising a nucleotide sequence to which nucleotides 4535496 to 4535476 and nucleotides 4489584 to 4489561 of the nucleotide sequence as shown in SEQ ID NO: 1 have been ligated; hereafter referred to as “primer E”) and DNA comprising the nucleotide sequence as shown in SEQ ID NO: 15 (DNA comprising a nucleotide sequence to which nucleotides 4489561 to 4489584 and nucleotides 4535476 to 4535496 of the nucleotide sequence as shown in SEQ ID NO: 1 have been ligated; hereafter referred to as “primer F”) were synthesized. PCR was carried out using, as a template, chromosomal DNA of the E. coli W3110 strain and, as primer sets, a pair of primer A and primer E and a pair of primer C and primer F. PCR was carried out with the use of a reaction solution of the same composition as above under the same reaction conditions, except for the use of a template and primer sets of different types.

The PCR-amplified DNA fragments were purified using a PCR Purification Kit and dissolved in 50 μl of water. The DNA fragments were confirmed to comprise 2-kb nucleotide sequences homologous to the nucleotide sequences of chromosomal DNA at both ends of the target region for deletion. Hereafter, the DNA fragment amplified with the use of the pair of primer A and primer E is referred to as an upstream arm and the DNA fragment amplified with the use of the pair of primer C and primer F is referred to as a downstream arm.

Subsequently, PCR was carried out using, as a template, a mixture of the upstream arm and the downstream arm obtained above and, as a primer set, DNA comprising the nucleotide sequence as shown in SEQ ID NO: 12 and DNA comprising the nucleotide sequence as shown in SEQ ID NO: 13. PCR was carried out with the use of a reaction solution of the same composition as above under the same reaction conditions, except for the use of, as templates, 1 μl of a solution of the upstream arm DNA and 1 μl of a solution of the downstream arm DNA obtained above and, as a primer set, DNA comprising the nucleotide sequence as shown in SEQ ID NO: 12 and DNA comprising the nucleotide sequence as shown in SEQ ID NO: 13.

The PCR-amplified DNA fragments were separated by agarose gel electrophoresis, purified using the QIA quick Gel Extraction Kit, and dissolved in 50 μl of water. It was confirmed that a DNA fragment that is an amplification product of a 2-kb fragment comprising a 1-kb fragment in the deleted region of the both arms was obtained (hereafter referred to as a “DNA fragment for deleting the sacB-cat gene”).

In accordance with the method of (2) (i) above, a solution of ΔE_suc competent cells was prepared, the DNA fragment for gene deletion was added thereto, and such DNA fragment was introduced into the ΔE_suc cell via electroporation. SuLB agar medium was used as a selection medium. The colonies of the selected transformants were replicated on an LBcm agar plate to select strains exhibiting chloramphenicol sensitivity.

Subsequently, PCR was carried out using, as a template, chromosomal DNA of the sucrose-resistant and chloramphenicol-sensitive strain selected above and, as primer set, the pair of primer A and primer C. PCR was carried out with the use of a reaction solution of the same composition as above under the same reaction conditions, except for the use of template DNA and primer DNA of different types.

Through the above PCR procedure, an amplified fragment of about 4.0 kb was obtained. An amplified DNA fragment is obtained via PCR using, as a template, chromosomal DNA of the sucrose-resistant and chloramphenicol-sensitive strain into which substitution of interest has been introduced and from which the suc gene and the cat gene have been deleted. Since a region comprising an upstream 2-kbp region and a downstream 2-kbp region adjacent to the deleted region is amplified from such DNA fragment, a band of about 4 kb is amplified. Accordingly, it was confirmed that a partially deleted strain (the ΔE strain) of chromosomal DNA that lacks a specific region on chromosomal DNA so as not to leave the marker gene behind was prepared by the above method.

(v) Preparation of Mutant Library of E. coli that Lacks Part of Chromosomal DNA

DNA that can be designed based on nucleotide sequences located at both ends of a target region of deletion on chromosomal DNA was used instead of the primers A to D above to prepare a mutant strain of E. coli that lacks an arbitrary region on chromosomal DNA of the E. coli W3110red strain in the same manner as described above.

EXAMPLE 2

Growth Test using Minimal Medium

Various mutant strains obtained in Example 1 were applied to an LB agar medium (which is a plate prepared with the addition of 15 g/l of agar to LB medium) and culture was conducted at 37° C. overnight to obtain cell strains. Small amounts of such strains were introduced into 5 ml of LB medium containing 50 mg/l of kanamycin and culturing was carried out overnight to obtain culture products. The culture products (50 μl) were inoculated into 5 ml of M9 minimal medium that contained 1% glucose and 10 mg/l of iron sulfate, shaking culture was conducted at 37° C., and turbidity 0, 6, 10, 13, 16, 19, and 32 hours after the initiation of culture was measured based on absorption at 660 nm. Growth of the above mutants and that of their parental strains, E. coli W3110red strains, were compared, and mutants exhibiting growth curves substantially the same as those of the parental strains were selected.

The deleted regions of chromosomal DNA of the selected mutants and genes included in such regions are shown in Table 2.

TABLE 2
Name of
deleted
region Genes contained in deleted region
Δ101B  JW0020(insB), JW0021(insA)
Δ102   JW0065(yabJ), JW0066(yabK), JW0067(tbpA), JW0068(yabN)
Δ103   JW0146(fhuA), JW0147(fhuC), JW0148(fhuD), JW0149(fhuB)
Δ001   JW0213(yafJ), JW0214(yafK), JW0215(yafQ), JW0216(dinJ), JW0217(yafL),
       JW0218(yafM), JW0219(fhiA), JW0220(lafU), JW0221(dinP), JW0222(yafN),
       JW0223(yafO), JW0224(yafP), JW0225(127#12), JW0226(prfH)
Δ002   JW0234(ykfI), JW0235(yafW), JW0235.5(yeeT), JW0236(ykfG), JW0237(yafX),
       JW0238(ykfF), JW0239(ykfB), JW0240(yafY), JW0241(ypjK), JW0242(yafZ),
       JW0243(ykfA), JW0244(perR), JW0245(129#3.1), JW0246(129#3.2), JW0247(129#5),
       JW0248(ykfC), JW0249(130#0.1), JW0250(is5B), JW0251(yi51), JW0252(mmuP),
       JW0253(mmuM), JW0254(afuC), JW0255(130#4), JW0256(insB), JW0257(insA),
       JW0258(130#5), JW0259(yagB), JW0260(yagA), JW0261(yagE), JW0262(yagF),
       JW0263(yagG), JW0264(yagH), JW0265(yagI), JW0266(argF), JW0267(yfjI),
       JW0268(insB), JW0269(insA), JW0270(yagJ), JW0271(yagK), JW0272(yagL),
       JW0273(yagM), JW0274(yagN), JW0275(intF), JW0276(yagP), JW0277(yagQ),
       JW0278(yagR), JW0279(yagS), JW0280(yagT), JW0281(yagU), JW0282(ykgJ),
       JW0283(yagV), JW0284(yagW), JW0285(yagX), JW0286(yagY), JW0287(yagZ),
       JW0288(ykgK), JW0289(ykgL), JW0290(ykgM), JW0291(eaeH), JW0292(134#4.1),
       JW0293(134#4.2), JW0294(ykgA), JW0295(ykgB), JW0296(ykgI), JW0297(merA),
       JW0298(ykgD), JW0299(ykgE), JW0300(ykgF), JW0301(ykgG), JW0302(ykgH),
       JW0303(betA), JW0304(betB), JW0305(betI), JW0306(betT), JW0307(yahA),
       JW0308(yahB), JW0309(yahC), JW0310(yahD), JW0311(yahE), JW0312(yahF),
       JW0313(yahG), JW0314(yahH), JW0315(arcM), JW0316(yahJ), JW0317(yahK),
       JW0318(yahL), JW0319(yahM), JW0320(yahN), JW0321(yahO), JW0322(prpR),
       JW0323(bcpA), JW0324(prpC), JW0325(prpD), JW0326(prpE), JW0327(codB),
       JW0328(codA), JW0329(cynR), JW0330(cynT), JW0331(cynS), JW0332(cynX),
       JW0333(lacA), JW0334(lacY), JW0335(lacZ), JW0336(lacI), JW0337(mhpR),
       JW0338(mhpA), JW0339(mhpB), JW0340(mhpC), JW0341(mhpD), JW0342(mhpF),
       JW0343(mhpE), JW0344(mhpT), JW0345(yaiL), JW0346(yaiM), JW0347(adhC),
       JW0348(yaiN), JW0349(yaiO), JW0350(140#9), JW0351(yi2A), JW0352(yi22),
       JW0353(yi24), JW0354(yi23), JW0355(yaiP), JW0356(yaiS), JW0357(tauA),
       JW0358(tauB), JW0359(tauC), JW0360(ssiD)
Δ007   JW0362(yaiT), JW0363(141#3.1), JW0364(141#3.2), JW0365(yaiU), JW0366(yaiV),
       JW0367(ampH), JW0368(sbmA), JW0369(yaiW), JW0370(yaiY), JW0371(yaiZ),
       JW0372(ddlA), JW0373(yaiB), JW0374(phoA), JW0375(psiF), JW0376(yaiC)
Δ009B  JW0482(ybbO), JW0483(tesA), JW0484(ybbA), JW0485(ybbP), JW0486(rhsD),
       JW0487(ybbC), JW0488(rhsE), JW0489(ybbD), JW0490(156#1), JW0491(ybbB),
       JW0492(ybbS), JW0493(ybbT), JW0494(ybbU)
Δ010   JW0497(ybbQ), JW0498(ybbV), JW0499(allP), JW0500(allA), JW0501(ybbY),
       JW0502(ybbZ), JW0503(ylbA), JW0504(allC), JW0505(ylbC), JW0506(fdrA),
       JW0507(ylbE), JW0508(ylbE), JW0509(ylbF), JW0510(arcC),
Δ011   JW0525(intD), JW0526(EXO_LAMBD), JW0527(VRPP_LAMBD), JW0528(159#4),
       JW0529(159#5), JW0530(VREN_LAMBD), JW0531(emrE), JW0532(ybcK),
       JW0533(ybcL), JW0534(ybcM), JW0535(ybcN), JW0536(ninE), JW0537(ybcO),
       JW0538(rus), JW0539(ybcQ), JW0539.5(160#10), JW0540(is5B), JW0541(yi51),
       JW0542(nmpC), JW0543(vlyS), JW0544(ybcS), JW0545(ybcT), JW0546(vboR),
       JW0547(ybcV), JW0548(ybcW), JW0549(nohB), JW0550(ybcX), JW0551(ybcY),
       JW0552(161#0.2), JW0553(appY), JW0554(ompT), JW0555(envY), JW0556(ybcH),
       JW0557(nfrA), JW0558(nfrB), JW0559(cusS), JW0560(cusR), JW0561(cusC),
       JW0562(ylcC), JW0563(cusB), JW0564(cusA), JW0565(pheP), JW0566(ybdG),
       JW0567(nfnB), JW0568(ybdF), JW0569(ybdJ), JW0570(ybdK), JW0571(hokE),
       JW0572(yi81), JW0573(yi82)
Δ012   JW0638(ybeL), JW0639(ybeQ), JW0640(ybeR), JW0641(ybeS), JW0642(ybeT),
       JW0643(ybeU), JW0644(ybeV), JW0645(ybeW), JW0646(ybeK), JW0647(gltL),
       JW0648(gltK), JW0649(gltJ), JW0650(ybeJ), JW0651(171#0.1), JW0652(is5B),
       JW0653(yi51)
Δ013B  JW0681(ybfK), JW0682(kdpE), JW0683(kdpD), JW0684(kdpC), JW0685(kdpB),
       JW0686(kdpA), JW0687(kdpF), JW0688(ybfA), JW0689(rhsC), JW0690(174#4.1),
       JW0691(ybfB), JW0692(rhsA), JW0693(ybfC), JW0694(ybfD), JW0695(ybfL),
       JW0696(ybfD), JW0697(ybgA)
Δ013   JW0687(kdpF), JW0688(ybfA), JW0689(rhsC), JW0690(174#4.1), JW0691(ybfB),
       JW0692(rhsA), JW0693(ybfC), JW0694(ybfD), JW0695(ybfL), JW0696(ybfD),
       JW0697(ybgA)
Δ104   JW0743(modF), JW0744(modE), JW0745(180#2), JW0746(modA), JW0747(modB),
       JW0748(modC)
Δ014   JW0782(204#3.1), JW0783(ybiA), JW0784(dinG), JW0785(ybiB), JW0786(ybiC),
       JW0787(ybiJ), JW0788(ybiI), JW0789(ybiX), JW0790(ybiL), JW0791(ybiM),
       JW0792(ybiN), JW0793(ybiO), JW0794(glnQ), JW0795(glnP), JW0796(glnH)
Δ015   JW0813(yliA), JW0814(yliB), JW0815(yliC), JW0816(yliD), JW0817(209#3),
       JW0818(209#4), JW0819(yliG), JW0820(yliH), JW0821(yliI), JW0822(yliJ),
       JW0823(dacC), JW0824(deoR), JW0825(ybjG)
Δ016   JW0834(ybjC), JW0835(mdaA), JW0836(rimK), JW0837(ybjN), JW0838(potF),
       JW0839(potG), JW0840(potH), JW0841(potI), JW0842(ybjO), JW0843(ybjF),
       JW0844(artJ), JW0845(artM), JW0846(artQ), JW0847(artI), JW0848(artP),
       JW0849(ybjP), JW0850(211#9), JW0851(211#10), JW0852(211#11), JW0853(ybjT),
       JW0854(ltaA)
Δ017C  JW0916(ssuB), JW0917(ssuC), JW0918(ssuD), JW0919(ssuA)
Δ018   JW0969(ymcC), JW0970(ymcD), JW0971(insA), JW0972(insB), JW0973(cspH),
       JW0974(cspG), JW0975(sfa), JW0976(yccL), JW0977(yccM), JW0978(torS),
       JW0979(torT), JW0980(torR), JW0981(torC), JW0982(torA), JW0983(torD),
       JW0984(yccD), JW0985(cbpA), JW0986(yccE), JW0987(agp), JW0988(yccJ),
       JW0989(wrbA), JW0990(yciG), JW0991(ycdG), JW0992(228#5), JW0993(228#6),
       JW0994(228#7), JW0995(ycdK), JW0996(228#9), JW0997(228#10), JW0998(ycdC),
       JW0999(putA), JW1000(yzpU), JW1001(putP), JW1002(229#1), JW1003(ycdO),
       JW1004(ycdB), JW1005(phoH), JW1006(ycdP), JW1007(ycdQ), JW1008(ycdR),
       JW1009(ycdR), JW1010(ycdS), JW1011(ycdT), JW1012(230#3), JW1013(230#3.1),
       JW1014(230#4), JW1015(ycdU)
Δ090   JW1105(ycfX), JW1106(238#9), JW1107(cobB), JW1108(ymfA), JW1109(potD),
       JW1110(potC), JW1111(potB), JW1112(potA), JW1113(pepT), JW1114(ycfD),
       JW1115(phoQ), JW1116(phoP)
Δ019   JW1123(ymfD), JW1124(ymfE), JW1125(lit), JW1126(intE), JW1127(VXIS_BPP21),
       JW1128(ymfH), JW1129(ymfI), JW1130(ymfJ), JW1131(RPC2_BPP22),
       JW1132(241#2), JW1133(ymfL), JW1134(ymfM), JW1135(ymfN), JW1136(ymfR),
       JW1137(ymfO), JW1138(241#8), JW1139(241#9), JW1140(ycfK), JW1141(ymfS),
       JW1142(ycfA), JW1143(ycfE), JW1144(pin), JW1145(mcrA), JW1146(241#16),
       JW1147(ycgW), JW1148(ycgX), JW1149(ycgE), JW1150(242#1), JW1151(ycgZ),
       JW1152(ymgA), JW1153(242#1.4), JW1154(ymgC), JW1155(yahA), JW1156(243#0.1),
       JW1157(243#1), JW1158(aidA), JW1159(ymgD), JW1160(243#4), JW1161(243#5),
       JW1162(243#5.1)
Δ020C  JW1166(ycgJ), JW1167(ycgK), JW1168(ycgL), JW1169(243#12), JW1170(ycgN),
       JW1171(clyA)
Δ091   JW1227(ychG), JW1227.5(251#6), JW1228(adhE), JW1229(ychE), JW1230(yi2A),
       JW1231(yi22), JW1232(yi24), JW1233(yi23), JW1234(251#8.5), JW1235(oppA),
       JW1236(oppB), JW1237(oppC), JW1238(oppD), JW1239(oppF), JW1240(yciU)
Δ022   JW1302(sucP), JW1303(258#8), JW1304(ycjO), JW1305(ycjP), JW1306(ycjQ),
       JW1307(259#3), JW1308(ycjS), JW1309(ycjT), JW1310(ycjU), JW1311(ycjV),
       JW1312(ompG), JW1313(ycjW), JW1314(ycjX), JW1315(ycjF), JW1316(tyrR),
       JW1317(tpx), JW1318(ycjG), JW1319(ycjI), JW1319.5(260#10), JW1320(ycjY),
       JW1321(ycjZ), JW1321.5(261#1.1), JW1322(mppA), JW1323(ynaI), JW1324(is5B),
       JW1325(yi51), JW1326(ynaJ), JW1327(ydaA), JW1328(fnr), JW1329(ogt),
       JW1330(ydaH), JW1331(261#9), JW1332(ydaJ), JW1333(262#1), JW1334(ydaL),
       JW1335(262#3), JW1336(262#4), JW1336.5(262#5), JW1337(dbpA), JW1338(ydaO),
       JW1339(intR), JW1340(ydaQ), JW1341(ydaC), JW1342(lar), JW1343(recT),
       JW1344(recE), JW1345(racC), JW1346(ydaE), JW1347(kil), JW1348(sieB),
       JW1349(ydaF), JW1350(263#6.1), JW1351(racR), JW1352(263#6.3), JW1353(ydaT),
       JW1354(ydaU), JW1355(dnaC), JW1356(ydaW), JW1357(enpP), JW1358(trkG),
       JW1359(ynaK), JW1360(ydaY), JW1361(VMTH_LAMBD), JW1362(VLOM_LAMBD),
       JW1363(is5B), JW1364(yi51), JW1365(VLOM_LAMBD), JW1366(Y401_LAMBD),
       JW1367(yzzL), JW1368(tnpO), JW1369(ynaE), JW1370(upo3),
Δ023   JW1376(ydbH), JW1377(ynbE), JW1378(ydbL), JW1379(feaR), JW1380(feaB),
       JW1381(tynA), JW1382(paaZ), JW1383(paaA), JW1384(paaB), JW1385(paaC),
       JW1386(paaD), JW1387(paaE)
Δ024   JW1393(paaK), JW1394(paaX), JW1395(paaY), JW1396(ydbA), JW1397(yi22),
       JW1398(yi24), JW1399(yi23), JW1400(yi2A), JW1401(268#0.5), JW1402(ydbB),
       JW1403(ydbC), JW1404(ydbD), JW1405(269#1), JW1406(ynbB), JW1407(269#3),
       JW1408(ynbD)
Δ025   JW1416(ydcA), JW1416.5(271#2), JW1417(trg), JW1418(ydcI), JW1419(271#5),
       JW1420(ydcG), JW1421(271#7), JW1422(ydcH), JW1423(rimL)
Δ026   JW1427(272#1), JW1427.5(272#2), JW1428(272#3), JW1429(272#4), JW1430(272#5),
       JW1431(prtC), JW1432(yncJ), JW1433(ydcQ), JW1434(yjiR), JW1435(273#4),
       JW1436(potA), JW1437(273#6), JW1438(potC), JW1439(ydcW), JW1440(ydcX),
       JW1441(ydcY), JW1442(ydcZ/ydcZ/ydcZ/ydcZ), JW1443(274#1), JW1444(yncB),
       JW1445(yncC), JW1446(yncD), JW1447(274#5), JW1448(ansP), JW1449(275#2),
       JW1450(275#2.1), JW1451(rhsE), JW1452(ydcD), JW1453(yhhI), JW1454(276#2.1),
       JW1455(ydcC), JW1456(ydcE), JW1457(yddH), JW1458(nhoA), JW1459(yddE)
Δ027   JW1478(yddO), JW1479(yddP), JW1480(yddQ), JW1481(yddR), JW1482(yddS),
       JW1483(280#1), JW1484(dos), JW1485(280#3), JW1486(280#4), JW1487(xasA)
Δ028B  JW1505(ydeW), JW1506(rbsA), JW1507(ydeY), JW1508(ydeZ), JW1509(304#1)
Δ029B  JW1533(ydfH), JW1534(ydfZ), JW1535(ydfI), JW1536(shiA), JW1537(307#4.1),
       JW1538(tnpR), JW1539(ydfM), JW1540(Y314_LAMBD), JW1541(nohA), JW1542(ydfO),
       JW1543(yccL), JW1544(cspI)
Δ030   JW1547(ydfR), JW1548(vlyS), JW1549(cspB), JW1550(cspF), JW1551(regQ_LAMBD),
       JW1552(309#4), JW1553(rem), JW1554(hokD), JW1555(relE), JW1556(relB),
       JW1557(ydfV), JW1558(flxA), JW1559(ydfW), JW1560(ydfX), JW1561(dicC)
Δ031C  JW1634(slyA), JW1635(ydhI), JW1636(yhcQ), JW1637(yhcP), JW1638(sodC),
       JW1639(ydhF), JW1640(ydhL), JW1641(ydhM), JW1642(nemA), JW1643(gloA)
Δ105A  JW1699(btuD), JW1700(btuE), JW1701(btuC)
Δ032C  JW1722(ydjC), JW1723(celF), JW1724(celD), JW1725(celC), JW1726(celB),
       JW1727(celA), JW1728(osmE)
Δ033C  JW1742(ynjA), JW1743(328#9), JW1744(ynjC), JW1745(328#11)
Δ034   JW1771(yeaF), JW1772(yeaG), JW1773(yeaH), JW1774(yeaI), JW1775(yeaJ),
       JW1776(yeaK), JW1777(332#0.1), JW1778(yeaL), JW1779(yeaM), JW1780(yeaN),
       JW1781(yeaO), JW1782(yoaF), JW1783(332#6), JW1784(333#1), JW1785(yoaG),
       JW1786(yeaR), JW1787(yeaS), JW1788(yeaT), JW1789(ttuC), JW1790(caiT),
       JW1791(yeaW), JW1792(yeaX)
Δ092   JW1806(manX), JW1807(manY), JW1808(manZ), JW1809(335#5), JW1810(yebN)
Δ036   JW1866(yecT), JW1867(flhE), JW1868(flhA), JW1869(flhB), JW1870(cheZ),
       JW1871(cheY), JW1872(cheB), JW1873(cheR), JW1874(tap), JW1875(tar),
       JW1876(cheW), JW1877(cheA), JW1878(motB), JW1879(motA), JW1880(flhC),
       JW1881(flhD), JW1882(340#3.1), JW1883(340#3.2), JW1884(yecG), JW1885(otsA),
       JW1886(otsB), JW1887(araH), JW1888(araG), JW1889(araF), JW1890(yecI),
       JW1891(yecJ), JW1892(341#5), JW1893(ftn), JW1894(yecH), JW1895(tyrP)
Δ037   JW1898(uvrC), JW1899(uvrY), JW1900(yecF), JW1901(sdiA), JW1902(yecC),
       JW1903(yecS), JW1904(yedO), JW1905(fliY), JW1906(fliZ), JW1907(fliA),
       JW1908(fliC), JW1909(fliD), JW1910(fliS), JW1911(fliT), JW1912(amyA),
       JW1913(yedD), JW1914(yedE), JW1915(yedF), JW1916(yedK), JW1917(yedL),
       JW1918(yedN), JW1919(yedN), JW1920(yedM), JW1921(fliE), JW1922(fliF),
       JW1923(fliG), JW1924(fliH), JW1925(fliI), JW1926(fliJ), JW1927(fliK), JW1928(fliL),
       JW1929(fliM), JW1930(fliN), JW1931(fliO), JW1932(fliP), JW1933(fliQ), JW1934(fliR),
       JW1935(rcsA), JW1936(dsrB), JW1937(344#6)
Δ038   JW1942(yedA), JW1943(vsr), JW1944(dcm), JW1945(yedJ), JW1946(344#15),
       JW1947(ompC), JW1948(344#17), JW1949(yedS), JW1950(yedU), JW1951(yedV),
       JW1952(copR), JW1953(yedX), JW1954(345#1), JW1955(345#2), JW1956(345#3)
Δ039   JW1972(is5B), JW1973(yi51), JW1974(348#3), JW1975(yi22), JW1976(yi24),
       JW1977(yi23), JW1978(yi2A), JW1979(348#3.5), JW1980(348#4), JW1981(yeeP),
       JW1982(flu), JW1983(349#2), JW1984(yeeS), JW1985(yeeT), JW1986(yeeU),
       JW1987(yeeV), JW1988(yeeW)
Δ040   JW2012(yefJ), JW2013(351#10), JW2014(is5B), JW2015(yi51), JW2016(yefJ),
       JW2017(yefI), JW2018(wbbJ), JW2019(yefG), JW2020(rfc), JW2021(glf),
       JW2022(rfbX), JW2023(rfbC), JW2024(rfbA), JW2025(rfbD), JW2026(rfbB)
Δ041   JW2052(yegE), JW2053(alkA), JW2054(yegD), JW2055(356#6), JW2056(356#7),
       JW2057(356#8), JW2058(356#9), JW2059(yegM), JW2060(yegN), JW2061(yegO),
       JW2062(yegB), JW2063(baeS), JW2064(baeR), JW2065(358#3), JW2066(yegQ),
       JW2067(ogrK), JW2068(358#6), JW2069(358#6.1), JW2070(yegS), JW2071(gatR),
       JW2072(358#8.1), JW2073(358#8.2), JW2074(gatR), JW2075(gatD), JW2076(gatC),
       JW2077(gatB), JW2078(gatA), JW2079(is5B), JW2080(yi51), JW2081(gatA),
       JW2082(gatZ), JW2083(gatY), JW2084(fbaB), JW2085(xegT), JW2086(yegU),
       JW2087(yegV), JW2088(yegW), JW2089(yegX)
Δ042   JW2102(molR), JW2103(molR), JW2104(molR), JW2105(yehI), JW2106(yehK),
       JW2107(yehL), JW2108(yehO), JW2109(yehP), JW2110(yehQ), JW2111(yehR),
       JW2112(yehS), JW2113(yehT), JW2114(yehU), JW2115(yehV), JW2116(yehW),
       JW2117(yehX), JW2118(yehY), JW2119(yehZ), JW2120(bglX), JW2121(dld),
       JW2122(pbpG), JW2123(yohC)
Δ093   JW2135(mglC), JW2136(mglA), JW2137(mglB), JW2138(galS)
Δ088   JW2147(yeiI), JW2148(yeiJ), JW2149(yeiK), JW2150(yeiL), JW2151(yeiM),
       JW2152(yeiN), JW2153(yeiC)
Δ043   JW2160(yeiQ), JW2161(yeiR), JW2162(yeiU), JW2163(spr), JW2164(rtn),
       JW2165(yejA), JW2166(yejB), JW2167(yejE), JW2168(yejF), JW2169(yejG),
       JW2170(bcr), JW2171(rsuA), JW2172(yejH)
Δ106A  JW2185(ccmE), JW2186(ccmD), JW2187(ccmC), JW2188(ccmB), JW2189(ccmA)
ΔK     JW2213(atoS), JW2214(atoC), JW2215(atoD), JW2216(atoA), JW2217(atoE),
       JW2218(atoB), JW2219(376#1), JW2220(376#2), JW2221(376#3), JW2222(376#4),
       JW2223(376#5)
Δ045   JW2261(elaB), JW2262(elaA), JW2263(elaC), JW2264(elaD), JW2265(yfbK),
       JW2266(yfbL), JW2267(yfbM), JW2268(yfbN), JW2269(403#0.1), JW2270(403#1)
Δ046   JW2295(yfcC), JW2296(406#2), JW2297(yfcE), JW2298(yfcF), JW2299(yfcG),
       JW2300(folX), JW2301(406#7), JW2302(yfcI), JW2303(hisP), JW2304(hisM),
       JW2305(hisQ), JW2306(hisJ), JW2307(argT)
Δ047   JW2345(yfdB), JW2346(411#1), JW2347(yfdH), JW2348(411#2.1), JW2349(ycfA),
       JW2350(yfdK), JW2351(yfdL), JW2352(yfdM), JW2353(xylU), JW2354(yfdN),
       JW2355(yfdO), JW2356(411#10), JW2357(411#11), JW2358(411#12),
       JW2359(411#13), JW2360(411#14)
Δ094   JW2377(yehU), JW2378(414#7), JW2379(yijO), JW2380(ptsA), JW2381(frvX),
       JW2382(yqhT), JW2383(ptwC), JW2384(ptwB)
Δ095   JW2527(yphA), JW2528(yphB), JW2529(yphC), JW2530(yphD), JW2531(yphE),
       JW2532(yphF), JW2533(yphG), JW2534(yphH)
Δ050   JW2602(intA), JW2603(yfjH), JW2604(alpA), JW2605(yfjI), JW2606(440#5.1),
       JW2607(yfjJ), JW2608(yfjK), JW2609(yfjL), JW2610(yfjM), JW2611(yfjN),
       JW2612(yfjO), JW2613(yfjP), JW2614(yfjQ), JW2615(yfjR), JW2616(ypjK),
       JW2617(yfjS), JW2618(yfjT), JW2619(yfjU), JW2620(yfjV), JW2621(yfjV),
       JW2622(442#2.2), JW2623(yfjW), JW2623.5(442#4), JW2624(yfjX), JW2625(yfjY),
       JW2626(yfjZ), JW2627(ypjF), JW2628(ypjA), JW2629(443#1.1), JW2630(443#1.2),
       JW2631(443#2), JW2632(443#2.1), JW2633(443#2.2), JW2634(443#3)
Δ051   JW2639(ygaE), JW2640(yzzM), JW2641(yqaE), JW2642(445#2), JW2643(ygaP),
       JW2644(stpA), JW2645(445#5), JW2646(ygaC), JW2647(ygaM), JW2648(nrdH),
       JW2649(nrdI), JW2650(nrdE)
Δ096   JW2652(proV), JW2653(proW), JW2654(proX), JW2655(445#15), JW2656(445#17),
       JW2657(ygaZ), JW2658(ygaH)
Δ097   JW2672(srlA), JW2673(srlB), JW2674(srlD), JW2675(gutM), JW2676(srlR),
       JW2677(gutQ)
Δ052   JW2723(iap), JW2724(ygbF), JW2725(452#12), JW2726(ygcH), JW2727(452#14),
       JW2728(ygcJ), JW2729(ygcK), JW2730(453#2), JW2731(ygcB)
Δ053   JW2735(ptpS), JW2736(ygcN), JW2737(ygcO), JW2738(ygcP), JW2739(ygcQ),
       JW2740(ygcR), JW2741(ygcS), JW2742(454#9), JW2743(ygcU), JW2744(ygcU),
       JW2745(ygcW), JW2746(yqcE), JW2747(ygcE), JW2748(ygcF), JW2749(ygcG)
Δ100   JW2753(chpA)
Δ054   JW2813(sdaC), JW2814(yqeH), JW2815(yqeI), JW2816(yqeJ), JW2817(yqeK),
       JW2818(ygeF), JW2819(ygeG), JW2820(ygeH), JW2821(ygeI), JW2822(x),
       JW2823(ygeK), JW2824(464#6), JW2825(464#7), JW2826(yi22), JW2827(yi24),
       JW2828(yi23), JW2829(yi2A), JW2830(465#0.1), JW2831(ygeP), JW2832(465#1),
       JW2833(nlpD), JW2834(yagR), JW2835(ygeT), JW2836(465#5), JW2837(ygeV),
       JW2838(ygeW), JW2839(dpaL), JW2840(ygeY), JW2841(ygeZ), JW2842(arcL),
       JW2843(yqeB), JW2844(466#6), JW2845(ygfJ), JW2846(gltD), JW2847(467#1),
       JW2848(ygfM), JW2849(yagR), JW2850(ygfO), JW2851(ygfP), JW2852(ygfQ),
       JW2853(ygfR), JW2854(ygfS), JW2855(ygfT), JW2856(pbuX), JW2857(idi)
Δ098   JW2900(cmtA), JW2901(cmtB)
Δ055   JW2935(epsM), JW2936(exeL), JW2937(exeC), JW2938(477#5), JW2939(477#6),
       JW2940(477#7), JW2941(478#1), JW2942(yghK), JW2943(glcB), JW2944(glcG),
       JW2945(glcF), JW2946(glcD), JW2947(glcC), JW2948(502#6), JW2949(is5B),
       JW2950(yi51), JW2951(yghQ), JW2952(yghR), JW2953(yghS), JW2954(yghT)
Δ056B  JW2988(ygiS), JW2989(ygiT), JW2990(ygiU), JW2991(506#5), JW2992(ygiW),
       JW2993(ygiX), JW2994(ygiY), JW2995(ygiZ), JW2996(mdaB), JW2997(ygiN)
Δ059   JW3046(ebgR), JW3047(ebgA), JW3048(ebgC), JW3049(ygjI), JW3050(ygjJ),
       JW3051(ygjK), JW3052(fadH), JW3053(ygjM), JW3054(ygjN), JW3055(ygjO),
       JW3056(ygjP), JW3057(ygjQ), JW3058(ygjR), JW3059(ygjT), JW3060(ygjU),
       JW3061(ygjV), JW3062(uxaA), JW3063(uxaC), JW3064(exuT), JW3065(exuR),
       JW3066(yqjA), JW3067(yqjB), JW3068(yqjC), JW3069(yqjD), JW3070(yqjE),
       JW3071(yqjK), JW3072(yqjF), JW3073(yqjG), JW3074(yhaH), JW3075(yhaI),
       JW3076(yhaJ), JW3077(yhaK), JW3078(yhaL), JW3079(yhaM), JW3080(yhaN),
       JW3081(yhaO), JW3082(yhaP), JW3083(yhaQ)
Δ060B  JW3093(yhaD), JW3094(yhaE), JW3095(yhaF), JW3096(yhaU), JW3097(yhaG),
       JW3098(sohA), JW3099(yhaV), JW3100(agaR), JW3101(agaZ), JW3102(agaV),
       JW3103(agaW), JW3104(agaA), JW3105(agaS), JW3106(agaY), JW3107(agaB),
       JW3108(agaC), JW3109(agaD), JW3110(agaI), JW3111(yraH), JW3112(yraI),
       JW3113(yraJ), JW3114(yraK), JW3115(yraL), JW3116(yraM), JW3117(yraN),
       JW3118(yraO), JW3119(yraP), JW3120(yraQ)
Δ061C  JW3159(yrbC), JW3160(yrbD), JW3161(yrbE), JW3162(yrbF)
Δ062   JW3181(gltF), JW3182(yhcA), JW3183(yhcD), JW3184(yhcE), JW3185(is5B),
       JW3186(yi51), JW3187(yhcE), JW3188(yhcF), JW3189(yhcG), JW3190(yhcH),
       JW3191(nanK), JW3192(nanE)
Δ064   JW3230(yhdJ), JW3231(yhdU), JW3232(envR), JW3233(acrE), JW3234(envD),
       JW3235(yhdV), JW3236(yhdW), JW3237(yhdX), JW3238(yhdY)
Δ065   JW3284(pinO), JW3285(gspA), JW3286(gspC), JW3287(gspD), JW3288(gspE),
       JW3289(hofF), JW3290(hofG), JW3291(hofH), JW3292(gspI), JW3293(gspJ),
       JW3294(gspK), JW3295(gspL), JW3296(pshM), JW3297(hofD), JW3298(bfr),
       JW3299(yheA), JW3300(chiA)
Δ068C  JW3401(gntR), JW3402(yhhW), JW3403(yhhX), JW3404(615#7.1), JW3405(yhhY),
       JW3406(yhhZ), JW3407(yrhA), JW3408(insA), JW3409(insB), JW3410(615#10.1),
       JW3411(yrhB), JW3412(ggt), JW3413(yhhA)
Δ068G  JW3419(livF), JW3420(livG), JW3421(livM), JW3422(livH), JW3423(livK)
Δ069   JW3447(rhsB), JW3448(610#6.1), JW3449(yhhH), JW3450(yibA), JW3451(yhhI),
       JW3452(yhhJ), JW3453(yhiH), JW3454(yhiI), JW3455(yhiJ), JW3456(yhiK),
       JW3457(yhiL), JW3458(yhiM), JW3459(yhiN)
Δ070F  JW3509(dppF), JW3510(dppD), JW3511(dppC), JW3512(dppB), JW3513(dppA)
Δ071B  JW3536(xylB), JW3537(xylA), JW3538(xylF), JW3539(xylG), JW3540(xylH)
Δ072   JW3548(yiaL), JW3549(yiaM), JW3550(yiaN), JW3551(yiaO), JW3552(lyx),
       JW3553(sgbH), JW3554(sgbU), JW3555(sgbE), JW3556(yiaT), JW3557(yiaU),
       JW3558(yiaV), JW3559(yiaW), JW3560(577#1.1), JW3561(aldB), JW3562(yiaY),
       JW3563(selB), JW3564(selA), JW3565(yibF), JW3566(rhsA), JW3567(576#2.1),
       JW3568(yibA), JW3569(yibJ), JW3570(yibG), JW3570.5(576#7), JW3571(yibH),
       JW3572(yibI)
Δ074   JW3650(yidF), JW3651(yidG), JW3652(yidH), JW3653(yidI), JW3654(yidJ),
       JW3655(yidK), JW3656(yidL), JW3657(567#9.1), JW3658(glvG), JW3659(glvB),
       JW3660(glvC), JW3661(yidP), JW3662(yidE)
Δ075   JW3665(yidQ), JW3666(yidR), JW3667(yidS), JW3668(dgoT), JW3669(dgoA),
       JW3670(dgoK), JW3671(dgoR), JW3672(yidW), JW3673(yidX), JW3674(yidA),
       JW3675(yidB)
Δ076   JW3722(asnA), JW3723(yieM), JW3724(559#4), JW3725(yieN), JW3726(kup),
       JW3727(rbsD), JW3728(rbsA), JW3729(rbsC), JW3730(rbsB), JW3731(rbsK),
       JW3732(rbsR), JW3733(yieO)
Δ078   JW3843(yihL), JW3844(yihM), JW3845(yihN), JW3846(yshA), JW3847(yihO),
       JW3848(yihP), JW3849(yihQ), JW3850(yihR), JW3851(yihS), JW3852(yihT),
       JW3853(yihU), JW3854(yihV), JW3855(yihW), JW3856(yihX), JW3857(rbn),
       JW3858(yihZ), JW3859(yiiD), JW3860(yiiE), JW3861(yiiF)
Δ079   JW3867(yiiG), JW3868(frvR), JW3869(frvX), JW3870(frvB), JW3871(frvA),
       JW3872(yiiL), JW3873(rhaD), JW3874(rhaA), JW3875(rhaB), JW3876(rhaS),
       JW3877(rhaR), JW3878(rhaT)
Δ080   JW3919(ptsA), JW3920(536#1), JW3921(frwC), JW3922(frwB), JW3923(pflD),
       JW3924(pflC), JW3925(frwD), JW3926(yijO), JW3927(yijP)
Δ081   JW3986(633#6), JW3987(yjbF), JW3988(yjbG), JW3989(yjbH), JW3989.5(633#10),
       JW3990(yjbA), JW3991(xylE), JW3992(malG), JW3993(malF), JW3994(malE),
       JW3995(malK), JW3996(lamB), JW3997(malM), JW3998(yjbI)
Δ082   JW4041(yjcP), JW4042(yjcQ), JW4043(yjcR), JW4044(yjcS), JW4045(alsK),
       JW4046(alsE), JW4047(alsC), JW4048(alsA), JW4049(alsB)
Δ083   JW4052(phnQ), JW4053(phnP), JW4054(phnO), JW4055(phnN), JW4056(phnM),
       JW4057(phnL), JW4058(phnK), JW4059(phnJ), JW4060(phnI), JW4061(phnH),
       JW4062(phnG), JW4063(phnF), JW4064(phnE), JW4065(phnE), JW4066(phnD),
       JW4067(phnC), JW4068(phnB), JW4069(phnA), JW4070(yjdA), JW4071(yjcZ)
Δ084   JW4139(yjfI), JW4140(yjfJ), JW4141(yjfK), JW4142(yjfL), JW4143(yjfM), JW4144(yjfC),
       JW4145(aidB), JW4146(yjfN), JW4147(yjfO), JW4148(yjfP), JW4149(yjfQ),
       JW4150(yjfR), JW4151(sgaT), JW4152(sgaB), JW4153(sgaA), JW4154(sgaH),
       JW4155(sgaU)
Δ108A  JW4186(ytfQ), JW4187(ytfR), JW4188(ytfS), JW4189(ytfT), JW4190(yjfF)
Δ099   JW4198(treC), JW4199(treB), JW4200(treR)
Δ085   JW4227(intB), JW4228(660#10.1), JW4229(yi2A), JW4230(yi22), JW4231(yi24),
       JW4232(yi23), JW4233(yjgW), JW4234(yjgX), JW4235(yjgY), JW4236(yjgZ),
       JW4237(yi41), JW4238(yi42), JW4239(yjhB), JW4240(yjhC), JW4241(yjhD),
       JW4242(yjhE), JW4243(yi91), JW4244(662#3.2), JW4245(662#4), JW4246(662#5)
Δ086   JW4254(insA), JW4255(insB), JW4256(insB), JW4257(yjhU), JW4258(yjhF),
       JW4259(yjhG), JW4260(yjhH), JW4261(yjhI), JW4262(sgcR), JW4263(sgcE),
       JW4264(sgcA), JW4265(sgcQ), JW4266(sgcC), JW4266.5(664#6), JW4267(sgcX),
       JW4268(yjhP), JW4269(yjhQ), JW4269.5(664#11), JW4270(664#12),
       JW4270.5(664#13), JW4271(yjhR), JW4272(yjhS), JW4273(yjhT), JW4274(yjhA),
       JW4275(fimB), JW4276(fimE), JW4277(fimA), JW4278(fimI), JW4279(fimC),
       JW4280(fimD), JW4281(fimF), JW4282(fimG), JW4283(fimH), JW4284(gntP),
       JW4285(uxuA), JW4286(uxuB), JW4287(uxuR), JW4288(yjiC), JW4289(yjiD),
       JW4290(yjiE), JW4291(iadA), JW4292(yjiG), JW4293(yjiH), JW4294(yjiI), JW4295(yjiJ),
       JW4296(yjiK), JW4296.5(668#5), JW4297(yjiL), JW4298(yjiM), JW4299(yjiN),
       JW4300(yjiO), JW4301(yjiP), JW4302(yjiQ), JW4303(yjiR), JW4304(yjiS), JW4305(yjiT),
       JW4306(yjiV), JW4307(mcrD), JW4308(mcrC), JW4309(mcrB), JW4310(yjiW),
       JW4311(hsdS), JW4312(hsdM), JW4313(hsdR), JW4314(mrr), JW4315(yjiA),
       JW4316(yjiX), JW4317(yjiY), JW4318(tsr), JW4319(yjjL), JW4320(671#8),
       JW4321(yjjM), JW4322(yjjN), JW4323(mdoB), JW4324(yjjA)

EXAMPLE 3

Preparation of Mutant Strain of E. coli having Chromosomal DNA with Multiple Partial Deletions

Mutants of E. coli having chromosomal DNA with multiple partial deletions were prepared using E. coli W3 11 Ored strains and accumulating the deleted regions that would not affect growth, which had been identified in Example 2, in E. coli W3110red strains by repeating the procedure of Example 1. Table 3 shows deleted regions of mutants of E. coli having chromosomal DNA with multiple partial deletions.

EXAMPLE 4

Evaluation of Mutant Strain of E. coli having Chromosomal DNA with Multiple Partial Deletions by Culturing (1)

Various mutants of E. coli having chromosomal DNA with multiple partial deletions obtained in Example 3 were applied to LB agar medium and cultured at 30° C. overnight to obtain cell strains. Small amounts of such strains were inoculated into 1 ml of LB medium that contained 3% glucose, and culturing was carried out overnight to obtain culture products. The culture products (50 μl) were inoculated into 5 ml of LB medium that contained 3% glucose, and culturing was carried out at 30° C. for 24 hours. After the completion of culturing, turbidity was measured based on absorption at 590 nm. The results are shown in Table 3.

3
			Finally
		Total	reached
		deleted	turbid-
Strain		length	ity
name	Deleted region	(kbp)	(%)
W3110red	None	0.0	100
Step1	Δ018	48.1	102
Step2	Deleted region possessed by Step1, Δ055	72.4	106
Step3	Deleted region possessed by Step2, Δ081	117.7	94
	Δ082 Δ083
Step4	Deleted region possessed by Step3, Δ019	144.7	98
Step5	Deleted region possessed by Step4,	162.4	99
	Δ029B Δ030
Step6	Deleted region possessed by Step5,	236.0	96
	Δ009B Δ010, Δ011
Step7	Deleted region possessed by Step6, Δ078	279.8	106
	Δ079 Δ080
Step8	Deleted region possessed by Step7, Δ054	327.5	104
Step9	Deleted region possessed by Step8, Δ001	477.1	112
	Δ002 Δ007
Step10	Deleted region possessed by Step9, Δ074	510.4	123
	Δ075 Δ077
Step11	Deleted region possessed by Step10,	605.9	123
	Δ036 Δ037 Δ038 Δ039 Δ040
Step12	Deleted region possessed by Step11,	656.2	153
	Δ050 Δ051 Δ096 Δ097
Step13	Deleted region possessed by Step12, Δ062	666.0	155
Step14	Deleted region possessed by Step13,	744.4	189
	Δ041 Δ042 Δ043 Δ088
Step15	Deleted region possessed by Step14, Δ098	746.2	178
Step16	Deleted region possessed by Step15,	841.3	190
	Δ085 Δ086
Step17	Deleted region possessed by Step16, Δ013	852.1	180
Step18	Deleted region possessed by Step17, Δ084	865.7	195

As shown in Table 3, mutants of E. coli having chromosomal DNA with multiple partial deletions, the total extent of such deletion being 470 kbp or more, exhibited substantially increased turbidity 24 hours after culturing in relation to the total extent of deletion. Cell morphologies of E. coli mutants were the same as those of wild-type E. coli strains, and mutants of E. coli having chromosomal DNA with multiple partial deletions, and the total extent of such deletion being 470 kbp or more, were found to yield more cells via culturing than wild-type E. coli strains.

EXAMPLE 5

Evaluation of Mutant Strain of E. coli having Chromosomal DNA with Multiple Partial Deletions by Culturing (2)

In order to confirm that increase of cell mass confirmed in Example 4 does not solely result from the regions that had been newly deleted from various mutants, mutants resulting from deletions from the mutants shown in Table 3 were prepared in accordance with the methods described in Example 1 and Example 3, culturing was carried out in the same manner as in Example 4, and turbidity 24 hours after the initiation of culturing was measured. The results are shown in Table 4.

TABLE 4
			Finally
Strain		Total deleted	reached
name	Deleted region	length (kbp)	turbidity (%)
W3110red	None	0.0	100
Unit1	Δ018	48.1	102
Unit2	Δ055	24.2	107
Unit3	Δ081 Δ082 Δ083	45.3	93
Unit4	Δ019	27.0	108
Unit5	Δ029B Δ030	17.7	99
Unit6	Δ009B Δ010 Δ011	73.6	100
Unit7	Δ078 Δ079 Δ080	43.8	101
Unit8	Δ054	47.8	108
Unit9	Δ001 Δ002 Δ007	149.6	101
Unit10	Δ074 Δ075 Δ077	33.3	94
Unit11	Δ036 Δ037 Δ038 Δ039 Δ040	95.5	110
Unit12	Δ050 Δ051 Δ096 Δ097	50.3	96
Unit13	Δ062	9.8	100
Unit14	Δ041 Δ042 Δ043 Δ088	78.4	109
Unit15	Δ098	1.8	98
Unit16	Δ085 Δ086	95.1	94
Unit17	Δ013	10.8	102
Unit18	Δ084	13.6	93

As shown in Table 4, new deletions would not result in increased turbidity. Accordingly, the increased cell mass observed in Example 4 was not attained by accumulation of deletions that would increase the amount of cells. However, it was demonstrated that such increase resulted from multiple and simultaneous occurrence of deletions of chromosomal DNA that would not solely affect growth.

EXAMPLE 6

Preparation of Mutant Strain of E. coli having Chromosomal DNA with Multiple Partial Deletions (2)

The Step 18 strain shown in Table 3 was designated as a parent strain, and a strain to which deletion of the Δ043 region shown in Table 2 had been returned was prepared in the following manner.

In accordance with the method of Example 3, a strain that lacks Δ041 and Δ042 regions from the E. coli W3110red strain was obtained. Subsequently, a cassette comprising the sacB gene and the cat gene ligated thereto (hereafter referred to as “sacB_cat;” see FIG. 1) was introduced into the Δ088 region adjacent to the Δ043 region to obtain strains (hereafter referred to as the “E. coli W3110red Δ041, Δ042, and Δ088::sacB_cat strains”).

P1-transducing phages were prepared from the E. coli W3110red Δ041, Δ042, and Δ088::sacB_cat strains in accordance with the method described in Example 1 (1), the Stepl8 strains were transformed with the P1 phages in accordance with the method described in Example 1 (1), and chloramphenicol-resistant strains were obtained. Thus, strains comprising the sacB/cat cassettes inserted into the Δ088 regions were selected.

Among the selected Step 18 transformants, strains to which the Δ043 regions have been returned, were selected in accordance with the method for detecting deletions described in Example 1 (2) (iii). sacB_cat on chromosomal DNA of the selected strain was eliminated by the method described in Example 1 (2) (iv) to obtain a Step 18γ strain having chromosomal DNA that lacks regions shown in Table 5.

Subsequently, chromosomal DNA of the Step 18γ strain was further subjected to deletion to prepare E. coli strains by the method described in Example 1 and Example 3. Table 5 shows mutants of E. coli having chromosomal DNA with multiple partial deletions.

	TABLE 5
		Turbidity
		of culture
		product
	Total	(absolute value)
		deletion	After	After
		length	19	36
Strain name	Deleted region	(kbp)	hours	hours
Step18γ	Δ001 Δ002 Δ007 Δ009B	865.7	5.54	6.64
	Δ010 Δ011 Δ013 Δ018
	Δ019 Δ029B Δ030 Δ036
	Δ037 Δ038 Δ039 Δ040
	Δ041 Δ042 Δ088 Δ050
	Δ051 Δ096 Δ097 Δ054
	Δ098 Δ055 Δ062 Δ074
	Δ075 Δ077 Δ078 Δ079
	Δ080 Δ081 Δ082 Δ083
	Δ084 Δ085 Δ086
Step19	Deleted region possessed by	884.1	5.49	6.69
	Step18γ, Δ069
Step20	Deleted region possessed by	933.8	5.27	7.52
	Step19, Δ014 Δ015 Δ016
Step21	Deleted region possessed by	941.1	5.25	7.85
	Step20, Δ056B
Step22	Deleted region possessed by	955.4	5.50	7.57
	Step21, Δ012
Step23	Deleted region possessed by	985.2	5.35	7.61
	Step22, Δ046 Δ047 Δ094
Step24	Deleted region possessed by	1001.6	5.61	8.01
	Step23, Δ065
Step25	Deleted region possessed by	1010.8	5.43	7.69
	Step24, Δ045
Step26	Deleted region possessed by	1020.5	5.62	7.87
	Step25, Δ064
Step27	Deleted region possessed by	1020.8	5.52	7.90
	Step26, Δ100
Step28	Deleted region possessed by	1026.7	5.36	7.61
	Step27, Δ103

EXAMPLE 7

Evaluation of Culture of Mutant Obtained in Example 6 by Culturing

Various mutants obtained in Example 6 were applied to LB agar medium and cultured at 37° C. overnight to obtain cell strains. Small amounts of such strains were inoculated into 5 ml of LB medium containing 50 mg/l of kanamycin and culturing was carried out overnight to obtain culture products. The culture products (50 μl) were inoculated into 5 ml of M9 medium that contained 1% glucose, 5 mg/l of iron sulfate and 20 g/l of calcium carbonate, shaking culture was conducted at 37° C., and 0, 6, 10, 13, 16, 19, and 32 hours after the initiation of culturing, turbidity was measured based on absorption at 660 nm.

Table 5 shows turbidity 19 and 32 hours after the initiation of culturing. Mutants with total deleted regions exceeding 933 kbp continuously exhibited increases in turbidity 19 hours after the initiation of culture and thereafter, at which point increase in turbidity of the parent strain appeared to have stopped and entered into a stationary phase. That is, mutants of E. coli strains having chromosomal DNA with total deleted regions of 933 kbp or more and with multiple partial deletions were found to yield a greater number of cells via culturing than wild-type E. coli strains.

EXAMPLE 8

Preparation of Substance using Mutant Strain of E. coli having Chromosomal DNA with Multiple Partial Deletions 1

(1) Preparation of Threonine Auxotrophic Mutant

In accordance with the method of Example 1, threonine auxotrophic mutants that lack the threonine biosynthesis operon on chromosomal DNA of the E. coli W3110red strains (the thrABC gene comprising a nucleotide sequence comprising nucleotides 36 to 5175 of the nucleotide sequence as shown in SEQ ID NO: 1) were produced and designated as the E. coli W3110redΔT strains.

(2) Preparation of Mutant that Lacks Meta Gene and Comprises Threonine Biosynthesis Gene Introduced Therein

(i) Preparation of DNA Fragment for MetA Gene Deletion and thrABC Gene Introduction

PCR was carried out using, as a template, chromosomal DNA of the E. coli W3110red strain and, as a primer set, DNA comprising the nucleotide sequence as shown in SEQ ID NO: 16 and DNA comprising the nucleotide sequence as shown in SEQ ID NO: 17. Thus, a DNA fragment having the nucleotide sequence in the 5′ upstream region of the metA gene was amplified.

PCR was carried out using LA-Taq, and a reaction solution was prepared in accordance with the instructions included therein. Purified chromosomal DNA (10 ng) and 20 pmol of each primer DNA were added to the reaction solution, and PCR was carried out. PCR was carried out by incubating at 94° C. for 2 minutes, repeating a cycle of 94° C. for 15 seconds, 55° C. for 20 seconds, and 68° C. for 90 seconds 25 times, and then incubating at 72° C. for 10 minutes.

The above PCR procedure verified that a target DNA fragment (fragment 1) had been obtained. By the similar method, a DNA fragment (fragment 2) comprising a nucleotide sequence of the 3′ downstream region of the metA gene was obtained using DNA comprising the nucleotide sequence as shown in SEQ ID NO: 18 and DNA comprising the nucleotide sequence as shown in SEQ ID NO: 19 as primer DNAs.

Subsequently, PCR was carried out using, as a template, chromosomal DNA of the E. coli W3110red strain and, as a primer set, DNA comprising the nucleotide sequence as shown in SEQ ID NO: 20 and DNA comprising the nucleotide sequence as shown in SEQ ID NO: 21 to amplify a DNA fragment containing threonine operon (thrABC gene).

PCR was carried out using LA-Taq, and a reaction solution was prepared in accordance with the instructions included therein. Purified chromosomal DNA (10 ng) and 20 pmol of each primer DNA were added to the reaction solution, and PCR was carried out.

PCR was carried out by incubating at 94° C. for 2 minutes, repeating a cycle of 94° C. for 15 seconds, 55° C. for 20 seconds, and 68° C. for 5 minutes 25 times, and then incubating at 72° C. for 10 minutes.

The above PCR procedure verified that a DNA fragment containing the thrABC gene (fragment 3) had been obtained.

Also, PCR was carried out using, as a template, pHSG399 plasmid DNA (Takara Bio Inc.) and, as a primer set, DNA having the nucleotide sequence as shown in SEQ ID NO: 22 and DNA having the nucleotide sequence as shown in SEQ ID NO: 23 to amplify a DNA fragment containing a chloramphenicol-resistant gene (cat gene).

PCR was carried out using LA-Taq, and a reaction solution was prepared in accordance with the instructions included therein. Plasmid DNA (40 ng) and 20 pmol of each primer DNA were added to the reaction solution, and PCR was carried out.

PCR was carried out by incubating at 94° C. for 2 minutes, repeating a cycle of 94° C. for 15 seconds, 55° C. for 20 seconds, and 68° C. for 1 minute 25 times, and then heating at 72° C. for 10 minutes.

The above PCR procedure verified that a DNA fragment containing the chloramphenicol-resistant gene (fragment 4) had been obtained.

The fragments 1 to 4 obtained by PCR were purified using the QIA quick Gel Extraction Kit (Qiagen) and dissolved in 50 μl of water to obtain DNA solutions. Primer sets of DNA as shown in SEQ ID NO: 17 and DNA as shown in SEQ ID NO: 22, DNA as shown in SEQ ID NO: 19 and DNA as shown in SEQ ID NO: 21, and DNA as shown in SEQ ID NO: 20 and DNA as shown in SEQ ID NO: 23 used in PCR each have an annelining sequence, i.e., a complementary sequence.

PCR was carried out using as templates the fragments 1 to 4 obtained above and, as a primer set, DNA comprising the nucleotide sequence as shown in SEQ ID NO: 24 and DNA comprising the nucleotide sequence as shown in SEQ ID NO: 25 to amplify a DNA fragment comprising the threonine operon gene and the chloramphenicol-resistant gene ligated in the same transcription direction and comprising DNA having nucleotide sequences of the 5′ upstream region and the 3′ downstream region of the metA gene further ligated to both ends thereof.

PCR was carried out using LA-Taq, and a reaction solution was prepared in accordance with the instructions included therein. The DNA solutions (1 μl each) and 20 pmol of each primer DNA were added to the reaction solution, and PCR was carried out.

PCR was carried out by incubating at 94° C. for 2 minutes, repeating a cycle of 94° C. for 15 seconds, 55° C. for 20 seconds, and 68° C. for 7 minutes 25 times, and then incubating at 72° C. for 10 minutes.

The above PCR procedure verified that a target DNA fragment had been obtained (hereafter referred to as a “DNA fragment for threonine operon gene introduction”). The DNA fragment was purified using the QIA quick Gel Extraction Kit and dissolved in 50 μl of water to obtain a DNA solution.

(ii) Transformation of E. coli w3110redA Strains

The competent cells of the E. coli w3110redA strains obtained in (1) above were prepared in accordance with the method described in Example 1 (2) (i).

The DNA fragment (0.1 μg) used for threonine operon gene introduction obtained in (2) (i) above was added to 50 ,l of a solution of E. coli W3110redA competent cells, and electroporation was carried out using a 0.1 −cm cuvette at 1.8 kV and 25 μF.

Strains in which the metA gene region had been substituted with a DNA fragment for threonine operon gene introduction on chromosomal DNA were selected on an LBcm plate. From among the DNA fragments for threonine operon gene introduction obtained in (2)(i) above, strains into which mutation had been introduced at the time of PCR and into which threonine operon genes encoding inactive threonine biosynthesis proteins had been introduced were to be selected. To this end, strains that would grow on LB agar medium but would not grow on M9 met agar minimal medium (1% glucose, 0.6% sodium phosphate, 0.3% potassium dihydrogenphosphate, 0.05% sodium chloride, 0.1% ammonium chloride, 2 mmol/l of magnesium sulfate heptahydrate, 100 μmol/l of calcium chloride, 10 mg/l of ferric sulfate, and 100 mg/l of methionine), i.e., threonine auxotrophic mutants, were selected from among transformants selected on the LBcm plate. It was confirmed that such strains comprised inactive threonine biosynthesis genes inserted into the metA genes on chromosomal DNA and designated as the E. coli Thr1 strains (W3110redΔT, ΔmetA::thrABC).

(iii) Preparation of Threonine Operon having Desensitizing Mutation and Attenuator Mutation

PCR was carried out using, as a template, chromosomal DNA of the E. coli W3110red strain and, as a primer set, DNA comprising the nucleotide sequence as shown in SEQ ID NO: 26 and DNA comprising the nucleotide sequence as shown in SEQ ID NO: 27 to amplify a DNA fragment comprising part of the desensitizing thrA gene and a nucleotide sequence of the upstream region of the threonine operon. Specifically, DNA as shown in SEQ ID NO: 27 has the desensitizing mutation described in J. Bacteriol., 185, 5442-5461, 2003.

PCR was carried out using LA-Taq and a reaction solution was prepared in accordance with the instructions included therein. Chromosomal DNA (10 ng) and 20 pmol of each primer DNA were added to the reaction solution and PCR was then carried out.

PCR was carried out by incubating at 94° C. for 2 minutes, repeating a cycle of 94° C. for 15 seconds, 55° C. for 20 seconds, and 68° C. for 2 minutes and 30 seconds 25 times, and then incubating at 72° C. for 10 minutes.

The above PCR procedure verified that a target DNA fragment had been obtained. The DNA fragment was purified using the QIA quick Gel Extraction Kit and dissolved in 50 II of water to obtain a DNA solution.

Subsequently, PCR was carried out using, as a template, chromosomal DNA of the E. coli W3110red strain and, as a primer set, DNA comprising the nucleotide sequence as shown in SEQ ID NO: 20 and DNA comprising the nucleotide sequence as shown in SEQ ID NO: 28 to amplify a DNA fragment comprising part of the desensitising thrA gene and a nucleotide sequence downstream of the threonine operon. That is, DNA as shown in SEQ ID NO: 28 has a desensitizing mutation described in J. Bacteriol., 185, 5442-5461, 2003.

PCR was carried out using LA-Taq and a reaction solution was prepared in accordance with the instructions included therein. Chromosomal DNA (10 ng) and 20 pmol of each primer DNA were added to the reaction solution and PCR was then carried out.

PCR was carried out by incubating at 94° C. for 2 minutes, repeating a cycle of 94° C. for 15 seconds, 55° C. for 20 seconds, and 68° C. for 3 minutes and 30 seconds 25 times, and then incubating at 72° C. for 10 minutes.

The above PCR procedure verified that a target DNA fragment had been obtained. The DNA fragment was purified using the QIA quick Gel Extraction Kit and dissolved in 50 ml of water to obtain a DNA solution.

PCR was carried out using, as a template, a DNA fragment comprising upstream and downstream nucleotide sequences of the threonine operon containing part of the desensitising thrA gene obtained via PCR above and, as a primer set, DNA comprising the nucleotide sequence as shown in SEQ ID NO: 20 and DNA comprising the nucleotide sequence as shown in SEQ ID NO: 29 to amplify a threonine operon DNA fragment containing the desensitising thrA gene.

PCR was carried out using LA-Taq and a reaction solution was prepared in accordance with the instructions included therein. The DNA solution (1 μl each) and 20 pmol of each primer DNA were added to the reaction solution, and PCR was carried out.

PCR was carried out by incubating at 94° C. for 2 minutes, repeating a cycle of 94° C. for 15 seconds, 55° C. for 20 seconds, and 68° C. for 5 minutes 25 times, and then incubating at 72° C. for 10 minutes.

The above PCR procedure verified that a target DNA fragment had been obtained. The DNA fragment was purified using the QIA quick Gel Extraction Kit and dissolved in 50 μl of water to obtain a DNA solution.

Subsequently, PCR was carried out using, as a template, a threonine operon DNA fragment containing the desensitising thrA gene obtained above and, as a primer set, DNA comprising the nucleotide sequence as shown in SEQ ID NO: 29 and DNA comprising the nucleotide sequence as shown in SEQ ID NO: 30 to amplify a DNA fragment having a nucleotide sequence upstream of a threonine operon comprising an attenuator mutation. Specifically, DNA comprising the nucleotide sequence as shown in SEQ ID NO: 30 comprises an attenuator mutation of a threonine operon described in J. Mol. Biol., 183, 529-541, 1985.

PCR was carried out using LA-Taq and a reaction solution was prepared in accordance with the instructions included therein. A DNA solution of a template DNA fragment (1 μl) and 20 pmol of each primer DNA were added to the reaction solution, and PCR was carried out.

PCR was carried out by heating at 94° C. for 2 minutes, repeating a cycle of 94° C. for 15 seconds, 55° C. for 20 seconds, and 68° C. for 5 minutes 25 times, and then heating at 72° C. for 10 minutes.

The above PCR procedure verified that a target DNA fragment (fragment 5) had been obtained.

Subsequently, PCR was carried out using, as a template, a threonine operon DNA fragment containing the desensitising thrA gene obtained above and, as a primer set, DNA comprising the nucleotide sequence as shown in SEQ ID NO: 31 and DNA comprising the nucleotide sequence as shown in SEQ ID NO: 20 to amplify a DNA fragment having a nucleotide sequence downstream of a threonine operon comprising an attenuator mutation. That is, DNA comprising the nucleotide sequence as shown in SEQ ID NO: 31 comprises an attenuator mutation of threonine operon described in J. Mol. Biol., 183, 529-541, 1985.

PCR was carried out using LA-Taq and a reaction solution was prepared in accordance with the instructions included therein. A DNA solution of the template DNA fragment (1 μl) and 20 pmol of each primer DNA were added to the reaction solution, and PCR was carried out.

PCR was carried out by incubating at 94° C. for 2 minutes, repeating a cycle of 94° C. for 15 seconds, 55° C. for 20 seconds, and 68° C. for 4 minutes and 30 seconds 25 times, and then incubating at 72° C. for 10 minutes.

The above PCR procedure verified that a target DNA fragment (fragment 6) had been obtained.

Fragment 5 and fragment 6 obtained by the above PCR were purified using the QIA quick Gel Extraction Kit and dissolved in 50 μl of water to obtain DNA solutions.

PCR was carried out using as templates fragment 5 and fragment 6 obtained above and, as a primer set, DNA comprising the nucleotide sequence as shown in SEQ ID NO: 20 and DNA comprising the nucleotide sequence as shown in SEQ ID NO: 21 to amplify a DNA fragment having a highly active threonine operon comprising the desensitising thrA gene and an attenuator mutation for increasing the expression level.

PCR was carried out using LA-Taq and a reaction solution was prepared in accordance with the instructions included therein. The DNA solution containing fragment 5 and fragment 6 (1 μl each) and 20 pmol of each primer DNA were added to the reaction solution, and PCR was carried out.

PCR was carried out by incubating at 94° C. for 2 minutes, repeating a cycle of 94° C. for 15 seconds, 55° C. for 20 seconds, and 68° C. for 5 minutes 25 times, and then incubating at 72° C. for 10 minutes.

The above PCR procedure verified that a target DNA fragment had been obtained. The DNA fragment was purified using the QIA quick Gel Extraction Kit and dissolved in 50 μl of water to obtain a DNA solution.

(iv) Preparation of E. coli Strain Possessing Highly Active Threonine Operon Comprising Desensitising thrA Gene and Attenuator Mutation

Competent cells of the E. coli Thr1 strains prepared in (ii) above were prepared in accordance with the method described in Example 1 (2) (i).

A DNA fragment (0.1 μg) having the highly active threonine operon comprising the desensitising thrA gene and attenuator mutation obtained in (iii) above was added to 50 μl of a solution of competent cells of the E. coli Thr1 strain, and electroporation was carried out using a 0.1 cm cuvette at 1.8 kV and 25 μF.

A transformant in which an inactive threonine operon has been substituted with a highly active threonine operon comprising the desensitising thrA gene and an attenuator mutation on chromosomal DNA was selected as a strain that would grow without exhibiting threonine auxotrophy using M9 met agar minimal medium. Such strain was confirmed to comprise the highly active threonine operon comprising the desensitising thrA gene and attenuator mutation inserted into the meta gene on chromosomal DNA and designated as the E. coli Thr2 strain.

(3) Fermentative Production of Threonine

In accordance with the method described in Example 1 (1), P1 transducing phages were prepared from the E. coli Thr2 strains. With the use of such phages, the E. coli W3110red strains and E. coli mutant strains comprising chromosomal DNAs with multiple partial deletions, which had been prepared in Example 3 and Example 6 (Step 2, Step4, Step6, Step8, Step10, Step12, Step14, Step16, Step18γ, Step20, Step22, Step24, Step26, and Step28), were subjected to P1 phage-mediated transduction in accordance with the method described in Example 1 (1). Strains in which a highly active threonine operon comprising the desensitising thrA gene and attenuator mutation derived from the E. coli Thr2 strain have been inserted into the meta gene of the mutant strains were selected as chloramphenicol-resistant strains and designated as W3110redT, Step2T, Step4T, Step6T, Step8T, Step10T, Step12T, Step14T, Step16T, Step18γT, Step20T, Step22T, Step24T, Step26T, and Step28T, respectively.

The transformants were applied to the LBcm plate described in Example 1 and cultured at 37° C. overnight. Small amounts of cells were scraped from the plate and inoculated into a 300-ml conical flask with baffles containing 25 ml of threonine-producing medium (50 g/l of glucose, 20 g/l of ammonium sulfate, 1 g/l of monopotassium phosphate, 2 g/l of Bacto yeast extract, 0.5 g/l of magnesium sulfate heptahydrate, 5 mg/l of iron sulfate, 5 mg/l of manganese sulfate, 30 g/l of calcium carbonate, and 120 mg/l of methionine), followed by culturing in rotary shaker at 30° C. and 250 rpm for 48 hours. During culturing, glucose was added thereto to a concentration of 20 g/l therein 24 hours after the initiation of culturing, and glucose was also added to culture solutions of Step10T, Step12T, Step14T, Step16T, Step18γT, Step20T, Step22T, Step24T, Step26T, and Step28T to a concentration of 20 g/l therein, 36 hours after the initiation of culturing.

After the completion of culturing, threonine that had been accumulated in the culture solution was quantified using an amino acid analyzer (Dionex). Glucose concentration in the culture solution was quantified using a reagent for quantifying glucose (Kyowa Medex Co., Ltd.). The results are shown in Table 6.

TABLE 6
	Amount of strain at the
	completion of culture	Amount of accumulated
Strain name	(0D660 nm)	threonin (g/l)
W3110redT	19.3	4.4
Step2T	19.5	2.2
Step4T	19.6	4.8
Step6T	20.2	5.7
Step8T	20.3	3.2
Step10T	22.3	7.7
Step12T	22.3	10.5
Step14T	24.3	9.5
Step16T	24.2	10.2
Step18γT	25.0	8.8
Step20T	24.1	6.1
Step22T	24.7	11.4
Step24T	23.5	11.2
Step26T	23.6	10.1
Step28T	23.3	10.6

As is apparent from Table 6, the Step10 strain and the strains thereafter with the total length of chromosomal DNA deletion which exceeds 470 kb, in which the threonine biosynthetic gene has been reinforced, exhibits significant increase in the amount of threonine accumulated, as compared with a strain (W3110redT) in which the threonine biosynthetic gene of a strain comprising chromosomal DNA without deletions has been reinforced. Also, increase in the amount of threonine accumulated is equivalent to or higher than increase in cell mass increased. Accordingly, a mutant strain of E. coli comprising chromosomal DNA that is at least 470 kb shorter than that of a wild-type E. coli strain is useful as a host cell for substance production.

EXAMPLE 9

Production of Substances using Mutant Strain of E. coli Comprising Chromosomal DNA with Multiple Partial Deletions 2

Glutathione is a peptidic substance comprising 3 types of amino acids, i.e., glutamic acid, cysteine, and glycine. The 3 aforementioned amino acids are condensed by the actions of γ-glutamyl cysteine synthetase and glutathione synthetase and glutathione is biosynthesized. ATP is a coenzyme that is indispensable for this condensation. Hereafter, the E. coli mutant strain of the present invention is used as an ATP source to produce glutathione.

• (1) Obtaining of Glutathione Synthetase Gene

Plasmid DNA comprising the glutathione synthetase gene used in the experiment below was extracted from FERM BP-337 (Japanese Published Unexamined Patent Application No. 31690/90 (1990)).

Plasmid DNA comprising the glutathione synthetase gene can also be obtained in the following manner.

Specifically, in accordance with the methods described in Japanese Published Unexamined Patent Application No. 20196/83(1983) and J. Gen. Microbiol., 128, 1047-1052, 1982, the E. coli B strains were subjected to mutation by N-methyl-N′-nitrosoguanidine, mutants with attenuated glutathione synthesis were obtained, C912 was subjected to the same mutation, mutants with restored glutathione synthesis were obtained, and mutants in which γ-glutamyl cysteine synthetase inhibition by glutathione had been released are selected among therefrom (hereafter referred to as “inhibition-released strains”).

Subsequently, in accordance with the methods described in Japanese Published Unexamined Patent Application No. 31690/90 (1990) and Appl. Environ. Microbiol., 44, 1444-1448, 1982, the γ-glutamyl cysteine synthetase genes on the chromosome of the inhibition-released strain obtained by the above method were cloned into plasmid pBR322. Further, in accordance with the methods described in Japanese Published Unexamined Patent Application No. 31690/90(1990) and Agric. Biol. Chem., 47, 1381-1383, 1983, the glutathione synthetase genes on the chromosome of the inhibition-released strains were cloned into plasmid pBR322.

Subsequently, in accordance with the methods described in Japanese Published Unexamined Patent Publication No. 31690/90 (1990) and Bioprocess Technol., 19, 159-183, 1994, the 2 above cloned fragments were accumulated in the vector plasmid pBR325. Thus, pBR325-gshI.II disclosed in Japanese Published Unexamined Patent Application No. 31690/90(1990) or plasmid DNA having a glutathione synthetase gene equivalent to that of the former plasmid DNA can be constructed.

The pBR325-gshI.II obtained by the above method (hereafter referred to as “pGS600”) was used to transform the E. coli C600 strain by the calcium chloride method. The transformant was selected using an LBcm plate containing 20 μg/ml of chloramphenicol and designated as E. coli C600/pGS600.

The E. coli C600/pGS600 strain was subjected to shaking culture using 5 ml of LB liquid medium containing 3% glucose (LBG medium) at 30° C. for 24 hours. Subsequently, 4 ml of the resulting culture product was introduced into a conical flask containing 400 ml of fresh LBG medium and the culture product was subjected to shaking culture at 30° C. for 24 hours. The resulting culture product was centrifuged to precipitate cells, and the cells were washed twice with 100 ml of 100 mmol/l Tris-HCl buffer (0° C., pH 7.4).

The washed cells were stored at −80° C., the cells were dissolved with the addition of 10 ml of 100 mmol/l Tris-HCl buffer (pH 7.4) immediately before use, and the resultant was used as an enzyme solution.

• (2) Obtaining of E. coli W3110red Cell Strain and Step28 Cell Strain

The W3110red strains and the Step28 strains were each independently subjected to shaking culture using 2.5 ml of LB liquid medium containing 3% glucose (LBG medium) at 30° C. for 24 hours, and 200 μl of the resulting culture product was introduced into a conical flask containing 20 ml of fresh LBG medium, followed by shaking culture at 30° C. for 24 hours. Table 7 shows turbidity (OD 660 um) measured upon completion of culture.

The resulting culture products were centrifuged to precipitate cells, and the cells were washed twice with 5 ml of 100 mmol/l Tris-HCl buffer (0° C., pH 7.4).

To the resulting washed cells, 200 μl of a GT solution (20% glucose and 0.4% Triton X100) was added, and the resulting solution of cells was used as an ATP source.

• (3) Production of Glutathione

To 900 μl of a reaction solution (2% glucose, 20 mmol/l of magnesium sulfate, 20 mmol/l of dipotassium sulfate, 0.22 mmol/l of oxidized nicotinamide adenine dinucleotide, 0.14 mmol/l of flavin mononucleotide, 5 mmol/l of glutamic acid, 5 mmol/l of cysteine, 5 mmol/l of glycine, 0.4% Triton X-100, 15 mmol/l of monopotassium phosphate, and 200 mmol/l of MOPS buffer; pH 7.8), 100 tl of the enzyme solution obtained in (1) above and 100 μl of the solution of the E. coli W3110red strains or Step28 strains obtained in (2) above were added, and the reaction was allowed to proceed while shaking culture was performed at 30° C. for 90 minutes.

Glutathione that had been accumulated in the reaction solution was quantified using a glutathione quantification kit (Dojindo Laboratories). The results are shown in Table 7.

TABLE 7
	Amount of strain at the
	completion of culture	Amount of accumulated
Strain name	(turbidity 0D60 nm)	glutathione (mmol/l)
W3110red	2.2	0.18
Step28	3.6	1.19

As shown in Table 7, the cell mass of the Step28 strains, which were subjected to the reaction as ATP sources and were mutants of E. coli strains having chromosomal DNA with multiple partial deletions, were 1.6 times (i.e., 3.6/2.2) those of the E. coli W3110red strains having chromosomal DNA without deletions; however, the figure for the amount of glutathione accumulated was 6.6 times (i.e., 1. 19/0.18).

The E. coli mutant strain according to the present invention exhibits a higher activity for producing ATP per cell, i.e., a higher activity for reproducing ATP, than its parental strain, the E. coli W3110red strain without chromosomal DNA deletions. Therefore, the E. coli mutant strain according to the present invention was found to be useful for producing useful substances that require ATP in their biosynthetic pathways.

Industrial Applicability

Use of the E. coli mutant strain of the present invention as a host cell enables effective production of useful substances.

Free Text of the Sequence Listing

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Claims

1. An E. coli mutant strain comprising the following genes [1] and [2] or homologous genes thereof and comprising chromosomal DNA that is at least 470 kbp shorter than that of a wild-type E. coli strain, provided that when the following genes [1] and [2] or homologous genes thereof are not found in wild-type E. coli strains (“NC genes”), the E. coli mutant strain does not contain the NC gene. [1] thrA gene, thrB gene, thrc gene, talB gene, mog gene, htgA gene, dnaK gene, dnaJ gene, hokC gene, nhaA gene, nhaR gene, rpsT gene, ribF gene, ileS gene, lspA gene, fkpB gene, lytB gene, dapB gene, carA gene, carB gene, caiF gene, caie gene, caiD gene, caiC gene, caiB gene, caiA gene, caiT gene, fixA gene, fixB gene, fixC gene, fixX gene, kefC gene, folA gene, apaH gene, apaG gene, ksgA gene, pdxA gene, surA gene, imp gene, djlA gene, rluA gene, hepA gene, polB gene, araD gene, araA gene, araB gene, araC gene, tbpA gene, leuD gene, leuC gene, leuB gene, leuA gene, leuL gene, leuO gene, ilvI gene, ilvH gene, fruR gene, ftsL gene, ftsl gene, murE gene, murF gene, mraY gene, murD gene, ftsW gene, murG gene, murC gene, ddlB gene, ftsQ gene, ftsA gene, ftsZ gene, lpxC gene, secA gene, mutT gene, yacE gene, guaC gene, hofC gene, hofB gene, ppdD gene, nadC gene, ampD gene, ampE gene, aroP gene, pdhR gene, aceE gene, aceF gene, lpdA gene, acnB gene, speD gene, speE gene, gcd gene, hpt gene, yadF gene, panD gene, panC gene, panB gene, htrE gene, ecpD gene, folK gene, pcnB gene, dksA gene, sfsA gene, ligT gene, hrpB gene, ponB gene, hemL gene, pfs gene, dgt gene, htrA gene, dapD gene, glnD gene, map gene, rpsB gene, tsf gene, smb gene, rrf gene, dxr gene, rth gene, cdsA gene, yaeL gene, hlpA gene, lpxD gene, fabZ gene, lpxA gene, lpxB gene, rnhB gene, dnaE gene, accA gene, ldcC gene, mesJ gene, rof gene, cutF gene, proS gene, rcsF gene, abc gene, dniR gene, gloB gene, mhA gene, dnaQ gene, up18 gene, gmhA gene, pepD gene, gpt gene, crl gene, phoE gene, proB gene, proA gene, hemB gene, proC gene, aroL gene, aroM gene, araJ gene, sbcC gene, sbcD gene, phoB gene, phoR gene, brnQ gene, proY gene, malZ gene, queA gene, tgt gene, secD gene, secF gene, tsx gene, ribD gene, ribH gene, nusB gene, thiL gene, pgpA gene, dxs gene, ispA gene, xseB gene, thiI gene, thiJ gene, apbA gene, cyoE gene, cyoD gene, cyoC gene, cyoB gene, cyoA gene, ampG gene, bolA gene, tig gene, clpP gene, clpX gene, lon gene, hupB gene, cof gene, mdlA gene, mdlB gene, glnK gene, amtB gene, tesB gene, maa gene, hha gene, acrB gene, acrA gene, acrR gene, aefA gene, priC gene, apt gene, dnaX gene, recR gene, htpG gene, adk gene, hemH gene, aes gene, gsk gene, fsr gene, ushA gene, cueR gene, gcl gene, gip gene, purK gene, purE gene, ybbF gene, ppiB gene, cysS gene, folD gene, sfmA gene, sfmC gene, sfmD gene, sfmH gene, sfmF gene, fimZ gene, entD gene, fepA gene, fes gene, entF gene, fepE gene, fepC gene, fepG gene, fepD gene, fepB gene, entC gene, entE gene, entB gene, entA gene, cstA gene, dsbG gene, ahpC gene, ahpF gene, rnk gene, ma gene, citT gene, citG gene, citX gene, citF gene, citE gene, citD gene, citC gene, citA gene, citB gene, dcuC gene, dcuC gene, crcA gene, cspE gene, crcB gene, lipA gene, lipB gene, dacA gene, ripA gene, mrdB gene, mrdA gene, phpB gene, nadD gene, holA gene, rlpB gene, leuS gene, lnt gene, phoL gene, ubiF gene, asnB gene, nagD gene, nagC gene, nagA gene, nagB gene, nagE gene, glnS gene, fur gene, fldA gene, seqA gene, pgm gene, potE gene, speF gene, kdpE gene, kdpD gene, kdpC gene, kdpB gene, kdpA gene, phrB gene, end8 gene, abrB gene, gltA gene, sdhC gene, sdhD gene, sdhA gene, sdhB gene, sucA gene, sucB gene, sucC gene, sucD gene, farR gene, hrsA gene, cydA gene, cydB gene, tolQ gene, tolR gene, tolA gene, tolB gene, pal gene, nadA gene, pnuC gene, czcD gene, aroG gene, gpmA gene, galM gene, galK gene, galT gene, galE gene, modF gene, modE gene, modA gene, modB gene, modC gene, bioA gene, bioB gene, bioF gene, bioC gene, bioD gene, uvrB gene, moaA gene, moaB gene, moaC gene, moaD gene, moaE gene, rhlE gene, dps gene, ompX gene, mipB gene, moeB gene, moeA gene, mdfA gene, grxA gene, poxB gene, aqpZ gene, cspD gene, clpA gene, infA gene, aat gene, cydC gene, cydD gene, trxB gene, lrp gene, ftsK gene, lolA gene, serS gene, dmsA gene, dmsB gene, dmsC gene, pflA gene, pflB gene, focA gene, serC gene, aroA gene, cmk gene, rpsA gene, himD gene, msbA gene, lpxK gene, kdsB gene, smtA gene, mukF gene, kicA gene, mukB gene, aspC gene, ompF gene, asnS gene, pncB gene, pepN gene, ssuB gene, ssuC gene, ssuD gene, ssuA gene, ssuE gene, pyrD gene, uup gene, pqiA gene, pqiB gene, rmf gene, fabA gene, ompA gene, sulA gene, helD gene, mgsA gene, acyP gene, hyaA gene, hyaB gene, hyaC gene, hyaD gene, hyaE gene, hyaF gene, appC gene, appB gene, appA gene, csgG gene, csgF gene, csgE gene, csgD gene, csgB gene, csgA gene, csgC gene, sfaA gene, mdoG gene, mdoH gene, msyB gene, htrB gene, solA gene, dinI gene, pyrC gene, grxB gene, rimJ gene, mviM gene, mviN gene, flgN gene, flgM gene, flgA gene, flgB gene, flgC gene, flgD gene, flgE gene, flgF gene, flgG gene, flgH gene, flgI gene, flgJ gene, flgK gene, flgL gene, me gene, riuC gene, rpmF gene, plsX gene, fabH gene, fabD gene, fabG gene, acpP gene, fabF gene, pabC gene, tmk gene, holB gene, ptsG gene, fhuE gene, nagZ gene, ndh gene, mfd gene, ycfU gene, ycfV gene, ycfW gene, cobB gene, potD gene, potC gene, potB gene, potA gene, pepT gene, phoQ gene, phoP gene, purB gene, trmU gene, icdA gene, minE gene, minD gene, minC gene, clyA gene, umuD gene, umuC gene, dsbB gene, nhaB gene, fadR gene, dadA gene, dadX gene, ldcA gene, mltE gene, tagl gene, treA gene, dhaR gene, pth gene, prsA gene, ychB gene, hemM gene, hemA gene, prfA gene, hemK gene, kdsA gene, chaA gene, chaB gene, chaC gene, narL gene, narX gene, narK gene, narG gene, narH gene, narJ gene, narI gene, tpr gene, purU gene, sprE gene, galU gene, hns gene, tdk gene, adhE gene, oppA gene, oppB gene, oppC gene, oppD gene, oppF gene, cls gene, kch gene, tonB gene, trpA gene, trpB gene, trpC gene, trpD gene, trpE gene, trpL gene, btuR gene, sohB gene, topA gene, cysB gene, acnA gene, ribA gene, pgpB gene, pyrF gene, osmB gene, mb gene, envM gene, sapF gene, sapD gene, sapC gene, sapB gene, sapA gene, aldH gene, ordL gene, goaG gene, pspF gene, pspA gene, pspB gene, pspC gene, pspD gene, pspE gene, sucP gene, ompG gene, tyrR gene, tpx gene, mppA gene, fnr gene, ogt gene, dbpA gene, intR gene, lar gene, recT gene, recE gene, racC gene, kil gene, sieB gene, racR gene, dnaC gene, enpP gene, trkG gene, VMTH_LAMBD gene, VLOM_LAMBD gene, tnpO gene, upo3 gene, ompN gene, nifJ gene, hslJ gene, ldhA gene, feaR gene, feaB gene, tynA gene, paaZ gene, paaA gene, paaB gene, paaC gene, paaD gene, paaE gene, paaF gene, paaG gene, paaH gene, paaI gene, paaJ gene, paaK gene, paaX gene, paaY gene, acpD gene, hrpA gene, aldA gene, gapC gene, gapC gene, cybB gene, trg gene, rimL gene, tehA gene, tehB gene, prtC gene, potA gene, potC gene, ansP gene, nhoA gene, narV gene, narW gene, narY gene, narZ gene, narU gene, fdnG gene, fdnH gene, fdnI gene, adhP gene, sfcA gene, rpsV gene, osmC gene, dos gene, xasA gene, gadB gene, pqqL gene, chuR gene, hipA gene, hipB gene, tam gene, uxaB gene, marC gene, marR gene, marA gene, marB gene, dcp gene, lycV gene, dicA gene, dicB gene, rspB gene, rspA gene, speG gene, dmsB gene, dmsC gene, bioD gene, mlc gene, asr gene, ebr gene, pntB gene, pntA gene, arcD gene, rstA gene, rstB gene, tus gene, fumC gene, fumA gene, manA gene, uidC gene, uidB gene, uidA gene, uidR gene, hdhA gene, malI gene, malX gene, malY gene, add gene, blr gene, nth gene, gst gene, pdxY gene, tyrS gene, pdxH gene, slyB gene, slyA gene, sodC gene, nemA gene, gloA gene, mt gene, lhr gene, sodB gene, araJ gene, purR gene, cfa gene, ribE gene, ag43 gene, phsC gene, nrfC gene, pykF gene, lpp gene, sufS gene, aroD gene, ppsA gene, aroH gene, nlpC gene, btuD gene, btuE gene, btuC gene, himA gene, pheT gene, pheS gene, pheM gene, rplT gene, rpmI gene, infC gene, thrS gene, arp gene, pfkB gene, cedA gene, katE gene, celF gene, celD gene, celC gene, celB gene, celA gene, osmE gene, nadE gene, spy gene, astE gene, astB gene, astD gene, astA gene, cstC gene, xthA gene, gdhA gene, topB gene, selD gene, sppA gene, ansA gene, tdh gene, gapA gene, ttuC gene, caiT gene, rnd gene, fadD gene, slp gene, pabB gene, sdaA gene, manX gene, manY gene, manZ gene, rrmA gene, cspC gene, kdgR gene, tcmA gene, htpX gene, prc gene, pqiB gene, pphA gene, holE gene, exoX gene, ptrB gene, purT gene, eda gene, edd gene, zwf gene, pykA gene, mlt gene, znuA gene, znuC gene, ruvB gene, ruvA gene, ruvC gene, ntpA gene, aspS gene, bisZ gene, torY gene, cutC gene, argS gene, pgsA gene, amn gene, cbl gene, nac gene, erfK gene, cobT gene, cobS gene, cobU gene, gyrI gene, phsE gene, sbcB gene, hisL gene, hisG gene, hisD gene, hisC gene, hisB gene, hisH gene, hisA gene, hisF gene, hisI gene, wzz gene, ugd gene, gnd gene, galF gene, wcaM gene, wcaL gene, wcaK gene, wzxC gene, wcaJ gene, cpsG gene, cpsB gene, wcaI gene, wcaH gene, wcaG gene, gmd gene, wcaF gene, wcaE gene, wcaD gene, wcaC gene, wcaB gene, wcaA gene, wzb gene, wza gene, asmA gene, dcd gene, udk gene, thiD gene, thiM gene, mrp gene, metG gene, cdd gene, sanA gene, mglC gene, mglA gene, mglB gene, galS gene, folE gene, cirA gene, lysP gene, nfo gene, fruA gene, fruK gene, fruB gene, rplY gene, narP gene, ccmH gene, dsbE gene, ccmF gene, ccmE gene, ccmD gene, ccmC gene, ccmB gene, ccmA gene, napC gene, napB gene, napH gene, napG gene, napA gene, napD gene, napF gene, eco gene, alkB gene, ada gene, apbE gene, rcsB gene, rcsC gene, atoS gene, atoC gene, atoD gene, atoA gene, atoE gene, atoB gene, gyrA gene, ubiG gene, nrdA gene, nrdB gene, inaA gene, glpQ gene, glpT gene, glpA gene, glpB gene, glpC gene, cinA gene, ais gene, pmrD gene, menE gene, menC gene, menB gene, menD gene, menF gene, nuoN gene, nuoM gene, nuoL gene, nuoK gene, nuoJ gene, nuoI gene, nuoH gene, nuoG gene, nuoF gene, nuoE gene, nuoD gene, nuoB gene, nuoA gene, lrhA gene, ackA gene, pta gene, ubiX gene, purF gene, dedE gene, dedD gene, folC gene, accD gene, dedA gene, truA gene, usg1 gene, pdxB gene, div gene, fabB gene, ddpX gene, mepA gene, aroC gene, sixA gene, fadL gene, vacJ gene, dsdC gene, dsdX gene, dsdA gene, emrY gene, emrK gene, evgA gene, evgS gene, bifF gene, ddg gene, glk gene, mntH gene, nupC gene, gltX gene, xapR gene, xapB gene, xapA gene, lig gene, zipA gene, cysZ gene, cysK gene, ptsH gene, ptsI gene, gsr gene, pdxK gene, cysM gene, cysA gene, cysW gene, cysU gene, cysP gene, ucpA gene, ptbA gene, amiA gene, hemF gene, cchA gene, eutC gene, eutB gene, eutH gene, eutG gene, eutJ gene, eutE gene, cchB gene, eutI gene, talA gene, tktB gene, aegA gene, narQ gene, acrD gene, dapE gene, purC gene, nlpB gene, dapA gene, gcvR gene, bcp gene, hyfA gene, hyfB gene, hyfC gene, hyfD gene, hyfE gene, hyfF gene, hyfG gene, hyfH gene, hyfI gene, hyfJ gene, hyfR gene, focB gene, perM gene, yfgE gene, uraA gene, upp gene, purM gene, purN gene, ppk gene, ppx gene, guaA gene, guaB gene, xseA gene, hisS gene, gcpE gene, ndk gene, pbpC gene, sseA gene, sseB gene, pepB gene, fdx gene, hscA gene, hscB gene, iscU gene, iscS gene, iscR gene, suhB gene, csiE gene, dgxA gene, dgxB gene, hcaC gene, hcaB gene, hcaD gene, glyA gene, hmpA gene, glnB gene, purL gene, acpS gene, pdxJ gene, recO gene, era gene, rnc gene, lepB gene, lepA gene, rseC gene, rseB gene, rseA gene, rpoE gene, nadB gene, srmB gene, ung gene, trx2 gene, pssA gene, kgtP gene, clpB gene, rluD gene, pheL gene, pheA gene, tyrA gene, aroF gene, rplS gene, trmD gene, rimM gene, rpsP gene, ffh gene, grpE gene, recN gene, smpA gene, smpB gene, gabD gene, gabT gene, gabP gene, nrdF gene, emrR gene, emrA gene, emrB gene, gshA gene, csrA gene, alaS gene, oraA gene, recA gene, mltB gene, hypF gene, hydN gene, ascG gene, ascF gene, ascB gene, hyc gene, hycH gene, hycG gene, hycF gene, hycE gene, hycD gene, hycC gene, hycB gene, hycA gene, hypA gene, hypB gene, hypC gene, hypD gene, hypE gene, fhlA gene, mutS gene, prpB gene, rpoS gene, pcm gene, surE gene, ygbB gene, ygbP gene, ygbQ gene, cysC gene, cysN gene, cysD gene, iap gene, cysH gene, cysI gene, cysJ gene, ptpS gene, eno gene, pyrG gene, mazG gene, chpR gene, relA gene, barA gene, gud2 gene, gudT gene, mioC gene, syd gene, sdaB gene, exo gene, fucO gene, fucA gene, fucP gene, fuc gene, fucK gene, fucU gene, fucR gene, gcvA gene, nifS gene, mltA gene, amiC gene, argA gene, thyA gene, lgt gene, ptsP gene, mutH gene, tas gene, aas gene, galR gene, lysA gene, lysR gene, araE gene, kduD gene, kduI gene, lysS gene, prfB gene, recJ gene, dsbC gene, xerD gene, fldB gene, upl4 gene, bglA gene, gcvP gene, gcvH gene, gcvT gene, visC gene, ubiH gene, pepP gene, serA gene, rpiA gene, iciA gene, sbm gene, argK gene, fba gene, pgk gene, epd gene, tktA gene, speB gene, speA gene, metK gene, galP gene, sprT gene, endA gene, gshB gene, yqgF gene, ansB gene, mutY gene, mltC gene, nupG gene, speC gene, pitB gene, gsp gene, hybG gene, hybF gene, hybE gene, hybD gene, hybC gene, hybB gene, hybA gene, hoxK gene, exbD gene, exbB gene, metC gene, exsA gene, sufI gene, plsC gene, parC gene, parE gene, icc gene, tolC gene, ribB gene, glgS gene, rfaE gene, glnE gene, cca gene, bacA gene, folB gene, ttdA gene, ttdB gene, ygjD gene, rpsU gene, dnaG gene, rpoD gene, aer gene, ebgR gene, ebgA gene, ebgC gene, fadH gene, uxaA gene, uxaC gene, exuT gene, exuR gene, tdcE gene, tdcD gene, tdcC gene, tdcB gene, tdcA gene, tdcR gene, sohA gene, agaR gene, agaZ gene, agaV gene, agaW gene, agaA gene, agaS gene, agaY gene, agaB gene, agaC gene, agaD gene, agaI gene, mtr gene, deaD gene, nlpI gene, pnp gene, rpsO gene, truB gene, rbfA gene, infB gene, nusA gene, argG gene, secG gene, mrsA gene, folP gene, ftsH gene, ftsJ gene, greA gene, dacB gene, yhbZ gene, rpmA gene, rplU gene, ispB gene, nlp gene, murA gene, yrbI gene, yrbK gene, rpoN gene, ptsN gene, ptsO gene, mtgA gene, arcB gene, gltB gene, nanT gene, npl gene, nanR gene, dcuD gene, sspB gene, sspA gene, rpsI gene, rplM gene, hhoA gene, hhoB gene, mdh gene, argR gene, tldD gene, mg gene, mreD gene, mreC gene, mreB gene, accB gene, accC gene, panF gene, prmA gene, fis gene, aroE gene, smg gene, smf gene, def gene, fmt gene, sun gene, trkA gene, mscL gene, zntR gene, rplQ gene, rpoA gene, rpsD gene, rpsK gene, rpsM gene, rpmJ gene, secY gene, rplO gene, rpmD gene, rpsE gene, rplR gene, rplF gene, rpsH gene, rpsN gene, rplE gene, rplX gene, rplN gene, rpsQ gene, rpmC gene, rplP gene, rpsC gene, rplV gene, rpsS gene, rplB gene, rplW gene, rplD gene, rplC gene, rpsJ gene, tufA gene, fusA gene, rpsG gene, rpsL gene, fkpA gene, slyX gene, slyD gene, kefB gene, prkB gene, crp gene, argD gene, pabA gene, fic gene, ppiA gene, nirB gene, nirD gene, nirC gene, cysG gene, php gene, trpS gene, gph gene, rpe gene, dam gene, damX gene, aroB gene, aroK gene, hofQ gene, mrcA gene, pckA gene, envZ gene, ompR gene, greB gene, feoA gene, feoB gene, bioH gene, gntT gene, malQ gene, malP gene, malT gene, rtcA gene, rtcB gene, rtcR gene, glpR gene, glpG gene, glpE gene, glpD gene, glgP gene, glgA gene, glgC gene, glgX gene, glgB gene, asd gene, gntU gene, gntU gene, gntK gene, gntR gene, ggt gene, ugpQ gene, ugpC gene, ugpE gene, ugpA gene, ugpB gene, livF gene, livG gene, livM gene, livH gene, livK gene, livJ gene, rpoH gene, ftsX gene, ftsE gene, ftsY gene, atzN gene, nikA gene, nikB gene, nikC gene, nikD gene, nikE gene, pitA gene, uspB gene, uspA gene, prlC gene, gor gene, arsR gene, arsB gene, arsC gene, slp gene, hdeB gene, hdeA gene, hdeD gene, gadA gene, treF gene, kdgK gene, dctA gene, dppF gene, dppD gene, dppC gene, dppB gene, dppA gene, tag gene, bisC gene, cspA gene, glyS gene, glyQ gene, xylB gene, xylA gene, xylF gene, xylG gene, xylH gene, xylR gene, bax gene, malS gene, avtA gene, lyx gene, sgbH gene, sgbU gene, sgbE gene, aldB gene, selB gene, selA gene, mtlA gene, mtlD gene, mtlR gene, lldP gene, lldR gene, lldD gene, cysE gene, gpsA gene, secB gene, grxC gene, tdh gene, kbl gene, htrL gene, rfaD gene, rfaF gene, rfaC gene, rfaL gene, rfaK gene, rfaZ gene, rfaY gene, rfaJ gene, rfaI gene, rfaB gene, rfaS gene, rfaP gene, rfaG gene, rfaQ gene, kdtA gene, kdtB gene, mutM gene, rpmG gene, rpmB gene, radC gene, dfp gene, dut gene, ttk gene, pyrE gene, rph gene, dinD gene, gmk gene, rpoZ gene, spoT gene, spoU gene, recG gene, gltS gene, nlpA gene, uhpT gene, uhpC gene, uhpB gene, uhpA gene, ilvN gene, ilvB gene, ivbL gene, emrD gene, ibpB gene, ibpA gene, gyrB gene, recF gene, dnaN gene, dnaA gene, rpmH gene, rnpA gene, yidC gene, thdF gene, tnaL gene, tnaA gene, tnaB gene, bglB gene, bglF gene, bglG gene, phoU gene, pstB gene, pstA gene, pstC gene, pstS gene, glmS gene, glmU gene, atpC gene, uncD gene, atpG gene, atpA gene, atpH gene, atpF gene, atpE gene, atpB gene, atpI gene, gidB gene, gidA gene, mioC gene, asnC gene, pssR gene, ilvL gene, ilvG gene, ilvM gene, ilvE gene, ilvD gene, ilvA gene, ilvY gene, ilvC gene, ppiC gene, rep gene, gppA gene, rhlB gene, trxA gene, rhoL gene, rho gene, rfe gene, wzzE gene, nfrC gene, rffD gene, rffG gene, rffH gene, rffA gene, wzxE gene, rffT gene, wecG gene, aslB gene, aslA gene, hemY gene, hemX gene, hemD gene, hemC gene, cyaA gene, cyaY gene, dapF gene, xerC gene, uvrD gene, corA gene, radD gene, pldA gene, recQ gene, pldB gene, metR gene, metE gene, udp gene, ubiE gene, aarF gene, tatC gene, tatD gene, rfaH gene, ubiD gene, ubiB gene, fadA gene, fadB gene, pepQ gene, trkH gene, trkH gene, hemG gene, mobB gene, mobA gene, dsbA gene, polA gene, yihA gene, hemN gene, ntrC gene, ntrB gene, glnA gene, fdhE gene, fdoI gene, fdoH gene, fdoG gene, fdhD gene, sodA gene, kdgT gene, cpxA gene, cpxR gene, cpxP gene, pfkA gene, sbp gene, cdh gene, tpiA gene, fpr gene, glpX gene, glpK gene, glpF gene, menG gene, menA gene, hslU gene, hslV gene, ftsN gene, cytR gene, priA gene, rpmE gene, metJ gene, metB gene, metL gene, metF gene, katG gene, gldA gene, talC gene, ppc gene, argE gene, argC gene, argB gene, argH gene, oxyR gene, udhA gene, trmA gene, btuB gene, murI gene, murB gene, birA gene, coaA gene, tufB gene, secE gene, nusG gene, rplK gene, rplA gene, rplJ gene, rplL gene, rpoB gene, rpoC gene, htrC gene, thiH gene, thiG gene, thiS gene, thiF gene, thiE gene, thiC gene, rsd gene, hemE gene, nfi gene, hupA gene, hydH gene, hydG gene, purD gene, purH gene, metA gene, aceB gene, aceA gene, aceK gene, arp gene, iclR gene, metH gene, pepE gene, lysC gene, pgi gene, ubiC gene, ubiA gene, plsB gene, dgkA gene, lexA gene, dinF gene, qor gene, dnaB gene, alr gene, tyrB gene, napA gene, hobH gene, uvrA gene, ssb gene, soxS gene, soxR gene, acs gene, nrfA gene, nrfB gene, nrfC gene, nrflD gene, nrfE gene, nrfF gene, nrfG gene, gltP gene, fdhF gene, alsR gene, rpiB gene, proP gene, basS gene, basR gene, adiY gene, adi gene, melR gene, melA gene, melB gene, fumB gene, dcuB gene, dcuR gene, dcuS gene, lysU gene, cadA gene, cadB gene, cadC gene, cutA3 gene, cutA2 gene, cutA gene, dcuA gene, aspA gene, fxsA gene, groES gene, groEL gene, efp gene, ecnB gene, sugE gene, blc gene, ampC gene, frdD gene, frdC gene, frdB gene, frdA gene, genX gene, psd gene, yjeQ gene, yjeE gene, amiB gene, mutL gene, miaA gene, hfq gene, hflX gene, hflK gene, hflC gene, purA gene, vacB gene, sgaE gene, rpsF gene, priB gene, rpsR gene, rplI gene, fklB gene, cycA gene, cpdB gene, cysQ gene, msrA gene, chpS gene, chpB gene, ppa gene, fbp gene, mpl gene, pmbA gene, cybC gene, nrdG gene, nrdD gene, treC gene, treB gene, treR gene, mgtA gene, pyrI gene, pyrB gene, pyrBI gene, argI gene, valS gene, holC gene, xerB gene, idnR gene, idnT gene, idnO gene, idnD gene, idnK gene, fecE gene, fecD gene, fecC gene, fecB gene, fecA gene, fecR gene, fecI gene, dnaC gene, dnaT gene, bglJ gene, fluF gene, rsmC gene, holD gene, rimI gene, prfC gene, osmY gene, deoC gene, deoA gene, deoB gene, deoD gene, lplA gene, smp gene, serB gene, radA gene, nadR gene, slt gene, trpR gene, gpmG gene, rob gene, creA gene, creB gene, creC gene, creD gene, arcA gene, lasT gene, thrL gene; [2] acrE gene, argT gene, artI gene, artJ gene, artM gene, artP gene, artQ gene, bfr gene, chiA gene, chpA gene, dacC gene, deoR gene, dinG gene, elaA gene, elaB gene, elaC gene, elaD gene, envD gene, envR gene, fhuA gene, fhuB gene, fhuC gene, fhuD gene, folX gene, glnH gene, glnP gene, glnQ gene, gltJ gene, gltK gene, gltL gene, gspA gene, gspC gene, gspD gene, gspE gene, gspI gene, gspJ gene, gspK gene, gspL gene, hisJ gene, hisM gene, hisP gene, hisQ gene, hofD gene, hofF gene, hofG gene, hofH gene, ltaA gene, mdaA gene, mdaB gene, pinO gene, potF gene, potG gene, potH gene, potI gene, pshM gene, ptwB gene, ptwC gene, rhsB gene, rimK gene, xylU gene.

2. The E. coli mutant strain according to claim 1 comprising chromosomal DNA that comprises DNA having the nucleotide sequences as shown in [1] to [69] below of SEQ ID NO: 1 or DNA equivalent thereto. [1] a nucleotide sequence of nucleotides 1 to 167064 [2] a nucleotide sequence of nucleotides 172980 to 243964 [3] a nucleotide sequence of nucleotides 253411 to 261963 [4] a nucleotide sequence of nucleotides 387532 to 389138 [5] a nucleotide sequence of nucleotides 403704 to 518029 [6] a nucleotide sequence of nucleotides 532712 to 535504 [7] a nucleotide sequence of nucleotides 550216 to 563940 [8] a nucleotide sequence of nucleotides 608119 to 675103 [9] a nucleotide sequence of nucleotides 689375 to 728820 [10] a nucleotide sequence of nucleotides 739594 to 832551 [11] a nucleotide sequence of nucleotides 848090 to 867637 [12] a nucleotide sequence of nucleotides 883474 to 890997 [13] a nucleotide sequence of nucleotides 909380 to 1048887 [14] a nucleotide sequence of nucleotides 1097020 to 1198105 [15] a nucleotide sequence of nucleotides 1225147 to 1630911 [16] a nucleotide sequence of nucleotides 1641039 to 1642631 [17] a nucleotide sequence of nucleotides 1650223 to 1964523 [18] a nucleotide sequence of nucleotides 1993076 to 1996661 [19] a nucleotide sequence of nucleotides 2028210 to 2033448 [20] a nucleotide sequence of nucleotides 2044377 to 2069148 [21] a nucleotide sequence of nucleotides 2081927 to 2104392 [22] a nucleotide sequence of nucleotides 2116065 to 2146263 [23] a nucleotide sequence of nucleotides 2187856 to 2200668 [24] a nucleotide sequence of nucleotides 2229851 to 2255894 [25] a nucleotide sequence of nucleotides 2263492 to 2270439 [26] a nucleotide sequence of nucleotides 2286589 to 2386261 [27] a nucleotide sequence of nucleotides 2395472 to 2423364 [28] a nucleotide sequence of nucleotides 2434099 to 2472851 [29] a nucleotide sequence of nucleotides 2482483 to 2505074 [30] a nucleotide sequence of nucleotides 2514558 to 2755676 [31] a nucleotide sequence of nucleotides 2789481 to 2795173 [32] a nucleotide sequence of nucleotides 2800891 to 2804307 [33] a nucleotide sequence of nucleotides 2810148 to 2825313 [34] a nucleotide sequence of nucleotides 2830263 to 2910273 [35] a nucleotide sequence of nucleotides 2910574 to 2985361 [36] a nucleotide sequence of nucleotides 3033129 to 3077084 [37] a nucleotide sequence of nucleotides 3078917 to 3110109 [38] a nucleotide sequence of nucleotides 3134342 to 3165627 [39] a nucleotide sequence of nucleotides 3172941 to 3360736 [40] a nucleotide sequence of nucleotides 3370548 to 3411159 [41] a nucleotide sequence of nucleotides 3420879 to 3453020 [42] a nucleotide sequence of nucleotides 3469367 to 3618575 [43] a nucleotide sequence of nucleotides 3636917 to 3854661 [44] a nucleotide sequence of nucleotides 3865832 to 3867101 [45] a nucleotide sequence of nucleotides 3877011 to 3926658 [46] a nucleotide sequence of nucleotides 3938879 to 4059897 [47] a nucleotide sequence of nucleotides 4079660 to 4086450 [48] a nucleotide sequence of nucleotides 4099983 to 4139231 [49] a nucleotide sequence of nucleotides 4149710 to 4235358 [50] a nucleotide sequence of nucleotides 4251736 to 4298976 [51] a nucleotide sequence of nucleotides 4311512 to 4313317 [52] a nucleotide sequence of nucleotides 4329722 to 4409532 [53] a nucleotide sequence of nucleotides 4423152 to 4496201 [54] a nucleotide sequence of nucleotides 4509535 to 4517875 [55] a nucleotide sequence of nucleotides 4599638 to 4641433 [56] a nucleotide sequence of nucleotides 167065 to 172979 [57] a nucleotide sequence of nucleotides 675104 to 689374 [58] a nucleotide sequence of nucleotides 832552 to 848089 [59] a nucleotide sequence of nucleotides 867638 to 883473 [60] a nucleotide sequence of nucleotides 890998 to 909379 [61] a nucleotide sequence of nucleotides 2386262 to 2395471 [62] a nucleotide sequence of nucleotides 2423365 to 2434098 [63] a nucleotide sequence of nucleotides 2472852 to 2482482 [64] a nucleotide sequence of nucleotides 2505075 to 2514557 [65] a nucleotide sequence of nucleotides 2910274 to 2910573 [66] a nucleotide sequence of nucleotides 3165628 to 3172940 [67] a nucleotide sequence of nucleotides 3411160 to 3420878 [68] a nucleotide sequence of nucleotides 3453021 to 3469366 [69] a nucleotide sequence of nucleotides 3618576 to 3636916

3. The E. coli mutant strain according to claim 1 or 2 comprising chromosomal DNA that does not comprise yafJ gene, yafK gene, yafQ gene, dinJ gene, yafL gene, yafM gene, fhiA gene, lafU gene, dinP gene, yafN gene, yafO gene, yafP gene, JW0225 gene, prfH gene, ykfI gene, yafW gene, yeeT gene, ykfG gene, yafX gene, ykfF gene, ykfB gene, yafY gene, ypjK gene, yafZ gene, ykfA gene, perR gene, JW0245 gene, JW0246 gene, JW0247 gene, ykfC gene, JW0249 gene, is5B gene, yi51 gene, mmuP gene, mmuM gene, afuC gene, JW0255 gene, insB gene, insA gene, JW0258 gene, yagB gene, yagA gene, yagE gene, yagF gene, yagG gene, yagH gene, yagI gene, argF gene, yfjI gene, yagJ gene, yagK gene, yagL gene, yagM gene, yagN gene, intF gene, yagP gene, yagQ gene, yagR gene, yagS gene, yagT gene, yagU gene, ykgJ gene, yagV gene, yagw gene, yagX gene, yagY gene, yagZ gene, ykgK gene, ykgL gene, ykgM gene, eaeH gene, JW0292 gene, JW0293 gene, ykgA gene, ykgB gene, ykgI gene, merA gene, ykgD gene, ykgE gene, ykgF gene, ykgG gene, ykgH gene, betA gene, betB gene, betI gene, betT gene, yahA gene, yahB gene, yahC gene, yahD gene, yahE gene, yahF gene, yahG gene, yahH gene, arcM gene, yahJ gene, yahK gene, yahL gene, yahM gene, yahN gene, yahO gene, prpR gene, bcpA gene, prpC gene, prpD gene, prpE gene, codB gene, codA gene, cynR gene, cynT gene, cynS gene, cynX gene, lacA gene, lacY gene, lacZ gene, lacI gene, mhpR gene, mhpA gene, mhpB gene, mhpC gene, mhpD gene, mhpF gene, mhpE gene, mhpT gene, yaiL gene, yaiM gene, adhC gene, yaiN gene, yaiO gene, JW0350 gene, yi2A gene, yi22 gene, yi24 gene, yi23 gene, yaiP gene, yaiS gene, tauA gene, tauB gene, tauC gene, ssiD gene, yaiT gene, JW0363 gene, JW0364 gene, yaiU gene, yaiV gene, ampH gene, sbmA gene, yaiW gene, yaiY gene, yaiZ gene, ddlA gene, yaiB gene, phoA gene, psiF gene, yaiC gene, ybbO gene, tesA gene, ybbA gene, ybbP gene, rhsD gene, ybbC gene, rhsE gene, ybbD gene, JW0490 gene, ybbB gene, ybbS gene, ybbT gene, ybbU gene, ybbQ gene, ybbV gene, allP gene, allA gene, ybbY gene, ybbZ gene, ylbA gene, allC gene, ylbC gene, fdrA gene, ylbE gene, ylbF gene, arcC gene, intD gene, EXO_LAMBD gene, VRPP_LAMBD gene, JW0528 gene, JW0529 gene, VREN_LAMBD gene, emrE gene, ybcK gene, ybcL gene, ybcM gene, ybcN gene, ninE gene, ybcO gene, rus gene, ybcQ gene, JW0539.5 gene, nmpC gene, vlyS gene, ybcS gene, ybcT gene, vboR gene, ybcV gene, ybcW gene, nohB gene, ybcX gene, ybcY gene, JW0552 gene, appY gene, ompT gene, envY gene, ybcH gene, nfrA gene, nfrB gene, cusS gene, cusR gene, cusC gene, ylcC gene, cusB gene, cusA gene, pheP gene, ybdG gene, nfnB gene, ybdF gene, ybdJ gene, ybdK gene, hokE gene, yi81 gene, yi82 gene, ymcC gene, ymcD gene, cspH gene, cspG gene, sfa gene, yccL gene, yccM gene, torS gene, torT gene, torR gene, torC gene, torA gene, torD gene, yccD gene, cbpA gene, yccE gene, agp gene, yccJ gene, wrbA gene, yciG gene, ycdG gene, JW0992 gene, JW0993 gene, JW0994 gene, ycdK gene, JW0996 gene, JW0997 gene, ycdC gene, putA gene, yzpU gene, putP gene, JW1002 gene, ycdO gene, ycdB gene, phoH gene, ycdP gene, ycdQ gene, ycdR gene, ycdS gene, ycdT gene, JW1012 gene, JW1013 gene, JW1014 gene, ycdU gene, ymfD gene, ymfE gene, lit gene, intE gene, VXIS_BPP21 gene, ymfH gene, ymfI gene, ymfJ gene, RPC2_BPP22 gene, JW1132 gene, ymfL gene, ymfM gene, ymfN gene, ymfR gene, ymfO gene, JW1138 gene, JW1139 gene, ycfK gene, ymfS gene, ycfA gene, ycfE gene, pin gene, mcrA gene, JW1146 gene, ycgW gene, ycgX gene, ycgE gene, JW1150 gene, ycgZ gene, ymgA gene, JW1153 gene, ymgC gene, yahA gene, JW1156 gene, JW1157 gene, aidA gene, ymgD gene, JW1160 gene, JW1161 gene, JW1162 gene, ydfH gene, ydfZ gene, ydfI gene, shiA gene, JW1537 gene, tnpR gene, ydfM gene, Y314_LAMBD gene, nohA gene, ydfO gene, yccL gene, cspI gene, ydfR gene, vlyS gene, cspB gene, cspF gene, regQ_LAMBD gene, JW1552 gene, rem gene, hokD gene, relE gene, relB gene, ydfV gene, flxA gene, ydfW gene, ydfX gene, dicC gene, sdaC gene, yqeH gene, yqeI gene, yqeJ gene, yqeK gene, ygeF gene, ygeG gene, ygeH gene, ygeI gene, JW2822 gene, ygeK gene, JW2824 gene, JW2825 gene, JW2830 gene, ygeP gene, JW2832 gene, nlpD gene, yagR gene, ygeT gene, JW2836 gene, ygeV gene, ygeW gene, dpaL gene, ygeY gene, ygeZ gene, arcL gene, yqeB gene, JW2844 gene, ygfJ gene, gltD gene, JW2847 gene, ygfM gene, yagR gene, ygfO gene, ygfP gene, ygfQ gene, ygfR gene, ygfS gene, ygfT gene, pbuX gene, idi gene, epsM gene, exeL gene, exeC gene, JW2938 gene, JW2939 gene, JW2940 gene, JW2941 gene, yghK gene, glcB gene, glcG gene, glcF gene, glcD gene, glcC gene, JW2948 gene, yghQ gene, yghR gene, yghS gene, yghT gene, yihL gene, yihM gene, yihN gene, yshA gene, yihO gene, yihP gene, yihQ gene, yihR gene, yihS gene, yihT gene, yihU gene, yihV gene, yihW gene, yihX gene, rbn gene, yihZ gene, yiiD gene, yiiE gene, yiiF gene, yiiG gene, frvR gene, frvX gene, frvB gene, frvA gene, yiiL gene, rhaD gene, rhaA gene, rhaB gene, rhaS gene, rhaR gene, rhaT gene, ptsA gene, JW3920 gene, frwC gene, frwB gene, pflD gene, pflC gene, frwD gene, yijO gene, yijP gene, JW3986 gene, yjbF gene, yjbG gene, yjbH gene, JW3989.5 gene, yjbA gene, xylE gene, malG gene, malF gene, malE gene, malK gene, lamB gene, malM gene, yjbI gene, yjcP gene, yjcQ gene, yjcR gene, yjcS gene, alsK gene, alsE gene, alsC gene, alsA gene, alsB gene, phnQ gene, phnP gene, phnO gene, phnN gene, phnM gene, phnL gene, phnK gene, phnJ gene, phnI gene, phnH gene, phnG gene, phnF gene, phnE gene, phnD gene, phnC gene, phnB gene, phnA gene, yjdA gene and yjcZ gene, or homologous gene thereof.

4. The E. coli mutant strain according to claim 3 comprising chromosomal DNA that does not comprise DNA having the nucleotide sequences as shown in [1] to [18] below of SEQ ID NO: 1 or DNA equivalent thereto. [1] a nucleotide sequence of nucleotides 243965 to 253410 [2] a nucleotide sequence of nucleotides 261964 to 387531 [3] a nucleotide sequence of nucleotides 389139 to 403703 [4] a nucleotide sequence of nucleotides 518030 to 532711 [5] a nucleotide sequence of nucleotides 535505 to 550215 [6] a nucleotide sequence of nucleotides 563941 to 608118 [7] a nucleotide sequence of nucleotides 1048888 to 1097019 [8] a nucleotide sequence of nucleotides 1198106 to 1225146 [9] a nucleotide sequence of nucleotides 1630912 to 1641038 [10] a nucleotide sequence of nucleotides 1642632 to 1650222 [11] a nucleotide sequence of nucleotides 2985362 to 3033128 [12] a nucleotide sequence of nucleotides 3110110 to 3134341 [13] a nucleotide sequence of nucleotides 4059898 to 4079659 [14] a nucleotide sequence of nucleotides 4086451 to 4099982 [15] a nucleotide sequence of nucleotides 4139232 to 4149709 [16] a nucleotide sequence of nucleotides 4235359 to 4251735 [17] a nucleotide sequence of nucleotides 4298977 to 4311511 [18] a nucleotide sequence of nucleotides 4313318 to 4329721

5. The E. coli mutant strain according to claim 4 comprising chromosomal DNA that does not comprise yidF gene, yidG gene, yidH gene, yidI gene, yidJ gene, yidK gene, yidL gene, JW3657 gene, glvG gene, glvB gene, glvC gene, yidP gene, yidE gene, yidQ gene, yidR gene, yidS gene, dgoT gene, dgoA gene, dgoK gene, dgoR gene, yidW gene, yidX gene, yidA gene, yidB gene, asnA gene, yieM gene, JW3724 gene, yieN gene, kup gene, rbsD gene, rbsA gene, rbsC gene, rbsB gene, rbsK gene, rbsR gene and yieO gene, or homologous gene thereof.

6. The E. coli mutant strain according to claim 5 comprising chromosomal DNA that does not comprise DNA having the nucleotide sequences as shown in [1] to [3] below of SEQ ID NO: 1 or DNA equivalent thereto. [1] a nucleotide sequence of nucleotides3854662 to 3865831 [2] a nucleotide sequence of nucleotides3867102 to 3877010 [3] a nucleotide sequence of nucleotides 3926659 to 3938878

7. The E. coli mutant strain according to claim 6 comprising chromosomal DNA that does not comprise yecT gene, flhE gene, flhA gene, flhB gene, cheZ gene, cheY gene, cheB gene, cheR gene, tap gene, tar gene, cheW gene, cheA gene, motB gene, motA gene, flhC gene, flhD gene, JW1882 gene, JW1883 gene, yecG gene, otsA gene, otsB gene, araH gene, araG gene, araF gene, yecI gene, yecJ gene, JW1892 gene, ftn gene, yecH gene, tyrP gene, uvrC gene, uvrY gene, yecF gene, sdiA gene, yecC gene, yecS gene, yedO gene, fliY gene, fliZ gene, fliA gene, fliC gene, fliD gene, fliS gene, fliT gene, amyA gene, yedD gene, yedE gene, yedF gene, yedK gene, yedL gene, yedN gene, yedM gene, fliE gene, fliF gene, fliG gene, fliH gene, fliI gene, fliJ gene, fliK gene, fliL gene, fliM gene, fliN gene, fliO gene, fliP gene, fliQ gene, fliR gene, rcsA gene, dsrB gene, JW1937 gene, yedA gene, vsr gene, dcm gene, yedJ gene, JW1946 gene, ompC gene, JW1948 gene, yedS gene, yedU gene, yedV gene, copR gene, yedX gene, JW1954 gene, JW1955 gene, JW1956 gene, JW1974 gene, JW1979 gene, JW1980 gene, yeeP gene, flu gene, JW1983 gene, yeeS gene, yeeU gene, yeeV gene, yeeW gene, yefJ gene, JW2013 gene, yefJ gene, yefI gene, wbbJ gene, yefG gene, rfc gene, glf gene, rfbX gene, rfbC gene, rfbA gene, rfbD gene and rfbB gene, or homologous gene thereof.

8. The E. coli mutant strain according to claim 7 comprising chromosomal DNA that does not comprise DNA having the nucleotide sequences as shown in [1] to [5] below of SEQ ID NO: 1 or DNA equivalent thereto. [1] a nucleotide sequence of nucleotides 1964524 to 1993075 [2] a nucleotide sequence of nucleotides 1996662 to 2028209 [3] a nucleotide sequence of nucleotides 2033449 to 2044376 [4] a nucleotide sequence of nucleotides 2069149t to 2081926 [5] a nucleotide sequence of nucleotides 2104393 to 2116064

9. The E. coli mutant strain according to claim 8 comprising chromosomal DNA that does not comprise intA gene, yfjH gene, alpA gene, yfjI gene, JW2606 gene, yfjJ gene, yfjK gene, yfjL gene, yfjM gene, yfjN gene, yfjO gene, yfjP gene, yfjQ gene, yfjR gene, ypjK gene, yfjS gene, yfjT gene, yfjU gene, yfjV gene, JW2622 gene, yfjW gene, JW2623.5 gene, yfjX gene, yfjY gene, yfjZ gene, ypjF gene, ypjA gene, JW2629 gene, JW2630 gene, JW2631 gene, JW2632 gene, JW2633 gene, JW2634 gene, ygaE gene, yzzM gene, yqaE gene, JW2642 gene, ygaP gene, stpA gene, JW2645 gene, ygaC gene, ygaM gene, nrdH gene, nrdI gene, nrdE gene, proV gene, proW gene, proX gene, JW2655 gene, JW2656 gene, ygaZ gene, ygaH gene, srlA gene, srlB gene, srlD gene, gutM gene, srlR gene and gutQ gene, or homologous gene thereof.

10. The E. coli mutant strain according to claim 9 comprising chromosomal DNA that does not comprise DNA having the nucleotide sequences as shown in [1] to [4] below of SEQ ID NO: 1 or DNA equivalent thereto. [1] a nucleotide sequence of nucleotides 2755677 to 2789480 [2] a nucleotide sequence of nucleotides 2795174 to 2800890 [3] a nucleotide sequence of nucleotides 2804308 to 2810147 [4] a nucleotide sequence of nucleotides 2825314 to 2830262

11. The E. coli mutant strain according to claim 10 comprising chromosomal DNA that does not comprise gltF gene, yhcA gene, yhcD gene, yhcE gene, yhcF gene, yhcG gene, yhcH gene, nanK gene and nanE gene, or homologous gene thereof.

12. The E. coli mutant strain according to claim 11 comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 3360737 to 3370547 of SEQ ID NO: 1 or DNA equivalent thereto.

13. The E. coli mutant strain according to claim 12 comprising chromosomal DNA that does not comprise yegE gene, alkA gene, yegD gene, JW2055 gene, JW2056 gene, JW2057 gene, JW2058 gene, yegM gene, yegN gene, yegO gene, yegB gene, baeS gene, baeR gene, JW2065 gene, yegQ gene, ogrK gene, JW2068 gene, JW2069 gene, yegS gene, gatR gene, JW2072 gene, JW2073 gene, gatR gene, gatD gene, gatC gene, gatB gene, gatA gene, gatZ gene, gatY gene, fbaB gene, xegT gene, yegU gene, yegV gene, yegW gene, yegX gene, molR gene, yehI gene, yehK gene, yehL gene, yehO gene, yehP gene, yehQ gene, yehR gene, yehS gene, yehT gene, yehU gene, yehV gene, yehW gene, yehX gene, yehY gene, yehZ gene, bglX gene, dld gene, pbpG gene, yohC gene, yeiQ gene, yeiR gene, yeiU gene, spr gene, rtn gene, yejA gene, yejB gene, yejE gene, yejF gene, yejG gene, bcr gene, rsuA gene, yejH gene, yeiI gene, yeiJ gene, yeiK gene, yeiL gene, yeiM gene, yeiN gene and yeiC gene, or homologous gene thereof.

14. The E. coli mutant strain according to claim 13 comprising chromosomal DNA that does not comprise DNA having any of the nucleotide sequences as shown in [1] to [4] below of SEQ ID NO: 1 or DNA equivalent thereto. [1] a nucleotide sequence of nucleotides2l46264 to 2187855 [2] a nucleotide sequence of nucleotides 2200669 to 2229850 [3] a nucleotide sequence of nucleotides 2270440 to 2286588 [4] a nucleotide sequence of nucleotides 2255895 to 2263491

15. The E. coli mutant strain according to claim 14 comprising chromosomal DNA that does not copmrise cmtA gene, cmtB gene, or homologous gene thereof.

16. The E. coli mutant strain according to claim 15 comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 3077085 to 3078916 of SEQ ID NO: 1 or DNA equivalent thereto.

17. The E. coli mutant strain according to claim 16 comprising chromosomal DNA that does not comprise intB gene, JW4228 gene, yjgW gene, yjgX gene, yjgY gene, yjgZ gene, yi41 gene, yi42 gene, yjhB gene, yjhC gene, yjhD gene, yjhE gene, yi91 gene, JW4244 gene, JW4245 gene, JW4246 gene, yjhU gene, yjhF gene, yjhG gene, yjhH gene, yjhI gene, sgcR gene, sgcE gene, sgcA gene, sgcQ gene, sgcC gene, JW4266.5 gene, sgcx gene, yjhP gene, yjhQ gene, JW4269.5 gene, JW4270 gene, JW4270.5 gene, yjhR gene, yjhS gene, yjhT gene, yjhA gene, fimB gene, fimE gene, fimA gene, fimI gene, fimC gene, fimD gene, fimF gene, fimG gene, fimH gene, gntP gene, uxuA gene, uxuB gene, uxuR gene, yjiC gene, yjiD gene, yjiE gene, iadA gene, yjiG gene, yjiH gene, yjiI gene, yjiJ gene, yjiK gene, JW4296.5 gene, yjiL gene, yjiM gene, yjiN gene, yjiO gene, yjiP gene, yjiQ gene, yjiR gene, yjiS gene, yjiT gene, yjiV gene, mcrD gene, mcrC gene, mcrB gene, yjiW gene, hsdS gene, hsdM gene, hsdR gene, mrr gene, yjiA gene, yjiX gene, yjiY gene, tsr gene, yjjL gene, JW4320 gene, yjjM gene, yjjN gene, mdoB gene and yjjA gene, or homologous gene thereof.

18. The E. coli mutant strain according to claim 17 comprising chromosomal DNA that does not comprise DNA having the nucleotide sequences as shown in [1] and/or [2] below of SEQ ID NO: 1 or DNA equivalent thereto. [1] a nucleotide sequence of nucleotides 4496202 to 4509534 [2] a nucleotide sequence of nucleotides 4517876 to 4599637

19. The E. coli mutant strain according to claim 18 comprising chromosomal DNA that does not comprise kdpF gene, ybfA gene, rhsC gene, JW0690 gene, ybfB gene, rhsA gene, ybfC gene, ybfD gene, ybfL gene, ybfD gene and ybgA gene, or homologous gene thereof.

20. The E. coli mutant strain according to claim 19 comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 728821 to 739593 of SEQ ID NO: 1 or DNA equivalent thereto.

21. The E. coli mutant strain according to claim 20 comprising chromosomal DNA that does not comprise yjfI gene, yjfJ gene, yjfK gene, yjfL gene, yjfM gene, yjfC gene, aidB gene, yjfN gene, yjfO gene, yjfP gene, yjfQ gene, yjfR gene, sgaT gene, sgaB gene, sgaA gene, sgaH gene and sgaU gene, or homologous gene thereof.

22. The E. coli mutant strain according to claim 21 comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 4409533 to 4423151 of SEQ ID NO: 1 or DNA equivalent thereto.

23. An E. coli mutant strain comprising thrA gene, thrB gene, thrC gene, talB gene, mog gene, htgA gene, dnaK gene, dnaJ gene, hokC gene, nhaA gene, nhaR gene, rpsT gene, ribF gene, ileS gene, lspA gene, fkpB gene, lytB gene, dapB gene, carA gene, carB gene, caiF gene, caiE gene, caiD gene, caiC gene, caiB gene, caiA gene, caiT gene, fixA gene, fixB gene, fixC gene, fixX gene, kefC gene, folA gene, apaH gene, apaG gene, ksgA gene, pdxA gene, surA gene, imp gene, djlA gene, rluA gene, hepA gene, polB gene, araD gene, araA gene, araB gene, araC gene, tbpA gene, leuD gene, leuC gene, leuB gene, leuA gene, leuL gene, leuO gene, ilvI gene, ilvH gene, fruR gene, ftsL gene, ftsI gene, murE gene, murF gene, mraY gene, murD gene, ftsW gene, murG gene, murC gene, ddlB gene, ftsQ gene, ftsA gene, ftsZ gene, lpxC gene, secA gene, mutT gene, yacE gene, guaC gene, hofC gene, hofB gene, ppdD gene, nadC gene, ampD gene, ampE gene, aroP gene, pdhR gene, aceE gene, aceF gene, lpdA gene, acnB gene, speD gene, speE gene, gcd gene, hpt gene, yadF gene, panD gene, panC gene, panB gene, htrE gene, ecpD gene, folK gene, pcnB gene, dksA gene, sfsA gene, ligT gene, hrpB gene, ponB gene, hemL gene, pfs gene, dgt gene, htrA gene, dapD gene, glnD gene, map gene, rpsB gene, tsf gene, smb gene, rrf gene, dxr gene, rth gene, cdsA gene, yaeL gene, hlpA gene, lpxD gene, fabZ gene, lpxA gene, lpxB gene, rnhB gene, dnaE gene, accA gene, ldcC gene, mesJ gene, rof gene, cutF gene, proS gene, rcsF gene, abc gene, dniR gene, gloB gene, mhA gene, dnaQ gene, up18 gene, gmhA gene, pepD gene, gpt gene, crl gene, phoE gene, proB gene, proA gene, hemB gene, proC gene, aroL gene, aroM gene, araJ gene, sbcC gene, sbcD gene, phoB gene, phoR gene, brnQ gene, proY gene, malZ gene, queA gene, tgt gene, secD gene, secF gene, tsx gene, ribD gene, ribH gene, nusB gene, thiL gene, pgpA gene, dxs gene, ispA gene, xseB gene, thiI gene, thiJ gene, apbA gene, cyoE gene, cyoD gene, cyoC gene, cyoB gene, cyoA gene, ampG gene, bolA gene, tig gene, clpP gene, clpX gene, lon gene, hupB gene, cof gene, mdlA gene, mdlB gene, glnK gene, amtB gene, tesB gene, maa gene, hha gene, acrB gene, acrA gene, acrR gene, aefA gene, priC gene, apt gene, dnaX gene, recR gene, htpG gene, adk gene, hemH gene, aes gene, gsk gene, fsr gene, ushA gene, cueR gene, gcl gene, gip gene, purK gene, purE gene, ybbF gene, ppiB gene, cysS gene, folD gene, sfmA gene, sfmC gene, sfmD gene, sfmH gene, sfmF gene, fimZ gene, entD gene, fepA gene, fes gene, entF gene, fepE gene, fepC gene, fepG gene, fepD gene, fepB gene, entC gene, entE gene, entB gene, entA gene, cstA gene, dsbG gene, ahpC gene, ahpF gene, mk gene, ma gene, citT gene, citG gene, citX gene, citF gene, citE gene, citD gene, citC gene, citA gene, citB gene, dcuC gene, dcuC gene, crcA gene, cspE gene, crcB gene, lipA gene, lipB gene, dacA gene, ripA gene, mrdB gene, mrdA gene, phpB gene, nadD gene, holA gene, rlpB gene, leuS gene, lnt gene, phoL gene, ubiF gene, asnB gene, nagD gene, nagC gene, nagA gene, nagB gene, nagE gene, glnS gene, fur gene, fldA gene, seqA gene, pgm gene, potE gene, speF gene, kdpE gene, kdpD gene, kdpC gene, kdpB gene, kdpA gene, phrB gene, end8 gene, abrB gene, gltA gene, sdhC gene, sdhD gene, sdhA gene, sdhB gene, sucA gene, sucB gene, sucC gene, sucD gene, farR gene, hrsA gene, cydA gene, cydB gene, tolQ gene, tolR gene, tolA gene, tolB gene, pal gene, nadA gene, pnuC gene, czcD gene, aroG gene, gpmA gene, galM gene, galK gene, galT gene, galE gene, modF gene, modE gene, modA gene, modB gene, modC gene, bioA gene, bioB gene, bioF gene, bioC gene, bioD gene, uvrB gene, moaA gene, moaB gene, moaC gene, moaD gene, moaE gene, rhlE gene, dps gene, ompX gene, mipB gene, moeB gene, moeA gene, mdfA gene, grxA gene, poxB gene, aqpZ gene, cspD gene, clpA gene, infA gene, aat gene, cydC gene, cydD gene, trxB gene, lrp gene, ftsK gene, lolA gene, serS gene, dmsA gene, dmsB gene, dmsC gene, pflA gene, pflB gene, focA gene, serC gene, aroA gene, cmk gene, rpsA gene, himD gene, msbA gene, lpxK gene, kdsB gene, smtA gene, mukF gene, kicA gene, mukB gene, aspC gene, ompF gene, asnS gene, pncB gene, pepN gene, ssuB gene, ssuC gene, ssuD gene, ssuA gene, ssuE gene, pyrD gene, uup gene, pqiA gene, pqiB gene, rmf gene, fabA gene, ompA gene, sulA gene, helD gene, mgsA gene, acyP gene, hyaA gene, hyaB gene, hyaC gene, hyaD gene, hyaE gene, hyaF gene, appC gene, appB gene, appA gene, csgG gene, csgF gene, csgE gene, csgD gene, csgB gene, csgA gene, csgC gene, sfaA gene, mdoG gene, mdoH gene, msyB gene, htrB gene, solA gene, dinI gene, pyrC gene, grxB gene, rimJ gene, mviM gene, mviN gene, flgN gene, flgM gene, flgA gene, flgB gene, flgC gene, flgD gene, flgE gene, flgF gene, flgG gene, flgH gene, flgI gene, flgJ gene, flgK gene, flgL gene, me gene, rluC gene, rpmF gene, plsX gene, fabH gene, fabD gene, fabG gene, acpP gene, fabF gene, pabC gene, tmk gene, holB gene, ptsG gene, fhuE gene, nagZ gene, ndh gene, mfd gene, ycfU gene, ycfV gene, ycfW gene, cobB gene, potD gene, potC gene, potB gene, potA gene, pepT gene, phoQ gene, phoP gene, purB gene, trmU gene, icdA gene, minE gene, minD gene, minC gene, clyA gene, umuD gene, umuC gene, dsbB gene, nhaB gene, fadR gene, dadA gene, dadX gene, ldcA gene, mltE gene, tagl gene, treA gene, dhaR gene, pth gene, prsA gene, ychB gene, hemM gene, hemA gene, prfA gene, hemK gene, kdsA gene, chaA gene, chaB gene, chaC gene, narL gene, narX gene, narK gene, narG gene, narH gene, narJ gene, narI gene, tpr gene, purU gene, sprE gene, galU gene, hns gene, tdk gene, adhE gene, oppA gene, oppB gene, oppC gene, oppD gene, oppF gene, cls gene, kch gene, tonB gene, trpA gene, trpB gene, trpC gene, trpD gene, trpE gene, trpL gene, btuR gene, sohB gene, topA gene, cysB gene, acnA gene, ribA gene, pgpB gene, pyrF gene, osmB gene, mb gene, envM gene, sapF gene, sapD gene, sapC gene, sapB gene, sapA gene, aldH gene, ordL gene, goaG gene, pspF gene, pspA gene, pspB gene, pspC gene, pspD gene, pspE gene, sucP gene, ompG gene, tyrR gene, tpx gene, mppA gene, fnr gene, ogt gene, dbpA gene, intR gene, lar gene, recT gene, recE gene, racC gene, kil gene, sieB gene, racR gene, dnaC gene, enpP gene, trkG gene, VMTH_LAMBD gene, VLOM_LAMBD gene, tnpO gene, upo3 gene, ompN gene, nifJ gene, hslJ gene, ldhA gene, feaR gene, feaB gene, tynA gene, paaZ gene, paaA gene, paaB gene, paaC gene, paaD gene, paaE gene, paaF gene, paaG gene, paaH gene, paaI gene, paaJ gene, paaK gene, paaX gene, paaY gene, acpD gene, hrpA gene, aldA gene, gapC gene, gapC gene, cybB gene, trg gene, rimL gene, tehA gene, tehB gene, prtC gene, potA gene, potC gene, ansP gene, nhoA gene, narV gene, narW gene, narY gene, narZ gene, narU gene, fdnG gene, fdnH gene, fdnI gene, adhP gene, sfcA gene, rpsV gene, osmC gene, dos gene, xasA gene, gadB gene, pqqL gene, chuR gene, hipA gene, hipB gene, tam gene, uxaB gene, marC gene, marR gene, marA gene, marB gene, dcp gene, lycV gene, dicA gene, dicB gene, rspB gene, rspA gene, speG gene, dmsB gene, dmsC gene, bioD gene, mlc gene, asr gene, ebr gene, pntB gene, pntA gene, arcD gene, rstA gene, rstB gene, tus gene, fumC gene, fumA gene, manA gene, uidC gene, uidB gene, uidA gene, uidR gene, hdhA gene, malI gene, malX gene, malY gene, add gene, blr gene, nth gene, gst gene, pdxY gene, tyrS gene, pdxH gene, slyB gene, slyA gene, sodC gene, nemA gene, gloA gene, mt gene, lhr gene, sodB gene, araJ gene, purR gene, cfa gene, ribE gene, ag43 gene, phsC gene, nrfC gene, pykF gene, lpp gene, sufS gene, aroD gene, ppsA gene, aroH gene, nlpC gene, btuD gene, btuE gene, btuC gene, himA gene, pheT gene, pheS gene, pheM gene, rplT gene, rpmI gene, infC gene, thrS gene, arp gene, pfkB gene, cedA gene, katE gene, celF gene, celD gene, ceIC gene, celB gene, celA gene, osmE gene, nadE gene, spy gene, astE gene, astB gene, astD gene, astA gene, cstC gene, xthA gene, gdhA gene, topB gene, selD gene, sppA gene, ansA gene, tdh gene, gapA gene, ttuC gene, caiT gene, md gene, fadD gene, slp gene, pabB gene, sdaA gene, manX gene, manY gene, manZ gene, rrmA gene, cspC gene, kdgR gene, tcmA gene, htpX gene, prc gene, pqiB gene, pphA gene, holE gene, exoX gene, ptrB gene, purT gene, eda gene, edd gene, zwf gene, pykA gene, mlt gene, znuA gene, znuC gene, ruvB gene, ruvA gene, ruvC gene, ntpA gene, aspS gene, bisZ gene, torY gene, cutC gene, argS gene, pgsA gene, amn gene, cbl gene, nac gene, erfK gene, cobT gene, cobS gene, cobU gene, gyrI gene, phsE gene, sbcB gene, hisL gene, hisG gene, hisD gene, hisC gene, hisB gene, hisH gene, hisA gene, hisF gene, hisI gene, wzz gene, ugd gene, gnd gene, galF gene, wcaM gene, wcaL gene, wcaK gene, wzxC gene, wcaJ gene, cpsG gene, cpsB gene, wcaI gene, wcaH gene, wcaG gene, gmd gene, wcaF gene, wcaE gene, wcaD gene, wcaC gene, wcaB gene, wcaA gene, wzb gene, wza gene, asmA gene, dcd gene, udk gene, thiD gene, thiM gene, mrp gene, metG gene, cdd gene, sanA gene, mglC gene, mglA gene, mglB gene, galS gene, folE gene, cirA gene, lysP gene, nfo gene, fruA gene, fruK gene, fruB gene, rplY gene, narP gene, ccmH gene, dsbE gene, ccmF gene, ccmE gene, ccmD gene, ccmC gene, ccmB gene, ccmA gene, napC gene, napB gene, napH gene, napG gene, napA gene, napD gene, napF gene, eco gene, alkB gene, ada gene, apbE gene, rcsB gene, rcsC gene, atoS gene, atoC gene, atoD gene, atoA gene, atoE gene, atoB gene, gyrA gene, ubiG gene, nrdA gene, nrdB gene, inaA gene, glpQ gene, glpT gene, glpA gene, glpB gene, glpC gene, cinA gene, ais gene, pmrD gene, menE gene, menC gene, menB gene, menD gene, menF gene, nuoN gene, nuoM gene, nuoL gene, nuoK gene, nuoJ gene, nuoI gene, nuoH gene, nuoG gene, nuoF gene, nuoE gene, nuoD gene, nuoB gene, nuoA gene, lrhA gene, ackA gene, pta gene, ubiX gene, purF gene, dedE gene, dedD gene, folC gene, accD gene, dedA gene, truA gene, usg1 gene, pdxB gene, div gene, fabB gene, ddpX gene, mepA gene, aroC gene, sixA gene, fadL gene, vacJ gene, dsdC gene, dsdX gene, dsdA gene, emrY gene, emrK gene, evgA gene, evgS gene, bifF gene, ddg gene, glk gene, mntH gene, nupC gene, gltX gene, xapR gene, xapB gene, xapA gene, lig gene, zipA gene, cysZ gene, cysK gene, ptsH gene, ptsI gene, gsr gene, pdxK gene, cysM gene, cysA gene, cysW gene, cysU gene, cysP gene, ucpA gene, ptbA gene, amiA gene, hemF gene, cchA gene, eutC gene, eutB gene, eutH gene, eutG gene, eutJ gene, eutE gene, cchB gene, eutI gene, talA gene, tktB gene, aegA gene, narQ gene, acrD gene, dapE gene, purC gene, nlpB gene, dapA gene, gcvR gene, bcp gene, hyfA gene, hyfB gene, hyfC gene, hyfD gene, hyfE gene, hyfF gene, hyfG gene, hyfH gene, hyfI gene, hyfJ gene, hyfR gene, focB gene, perM gene, yfgE gene, uraA gene, upp gene, purM gene, purN gene, ppk gene, ppx gene, guaA gene, guaB gene, xseA gene, hisS gene, gcpE gene, ndk gene, pbpC gene, sseA gene, sseB gene, pepB gene, fdx gene, hscA gene, hscB gene, iscU gene, iscS gene, iscR gene, suhB gene, csiE gene, dgxA gene, dgxB gene, hcaC gene, hcaB gene, hcaD gene, glyA gene, hmpA gene, glnB gene, purL gene, acpS gene, pdxJ gene, recO gene, era gene, mc gene, lepB gene, lepA gene, rseC gene, rseB gene, rseA gene, rpoE gene, nadB gene, srmB gene, ung gene, trx2 gene, pssA gene, kgtP gene, clpB gene, riuD gene, pheL gene, pheA gene, tyrA gene, aroF gene, rplS gene, trmD gene, rimM gene, rpsP gene, ffh gene, grpE gene, recN gene, smpA gene, smpB gene, gabD gene, gabT gene, gabP gene, nrdF gene, emrR gene, emrA gene, emrB gene, gshA gene, csrA gene, alaS gene, oraA gene, recA gene, mltB gene, hypF gene, hydN gene, ascG gene, ascF gene, ascB gene, hyc gene, hycH gene, hycG gene, hycF gene, hycE gene, hycD gene, hycC gene, hycB gene, hycA gene, hypA gene, hypB gene, hypC gene, hypD gene, hypE gene, fhlA gene, mutS gene, prpB gene, rpoS gene, pcm gene, surE gene, ygbB gene, ygbP gene, ygbQ gene, cysC gene, cysN gene, cysD gene, iap gene, cysH gene, cysI gene, cysJ gene, ptpS gene, eno gene, pyrG gene, mazG gene, chpR gene, relA gene, barA gene, gud2 gene, gudT gene, mioC gene, syd gene, sdaB gene, exo gene, fucO gene, fucA gene, fucP gene, fuc gene, fucK gene, fucU gene, fucR gene, gcvA gene, nifS gene, mltA gene, amiC gene, argA gene, thyA gene, lgt gene, ptsP gene, mutH gene, tas gene, aas gene, galR gene, lysA gene, lysR gene, araE gene, kduD gene, kduI gene, lysS gene, prfB gene, recJ gene, dsbC gene, xerD gene, fldB gene, up14 gene, bglA gene, gcvP gene, gcvH gene, gcvT gene, visC gene, ubiH gene, pepP gene, serA gene, rpiA gene, iciA gene, sbm gene, argK gene, fba gene, pgk gene, epd gene, tktA gene, speB gene, speA gene, metK gene, galP gene, sprT gene, endA gene, gshB gene, yqgF gene, ansB gene, mutY gene, mltC gene, nupG gene, speC gene, pitB gene, gsp gene, hybG gene, hybF gene, hybE gene, hybD gene, hybC gene, hybB gene, hybA gene, hoxK gene, exbD gene, exbB gene, metC gene, exsA gene, sufI gene, plsC gene, parC gene, parE gene, icc gene, tolC gene, ribB gene, glgS gene, rfaE gene, glnE gene, cca gene, bacA gene, folB gene, ttdA gene, ttdB gene, ygjD gene, rpsU gene, dnaG gene, rpoD gene, aer gene, ebgR gene, ebgA gene, ebgC gene, fadH gene, uxaA gene, uxaC gene, exuT gene, exuR gene, tdcE gene, tdcD gene, tdcC gene, tdcB gene, tdcA gene, tdcR gene, sohA gene, agaR gene, agaZ gene, agaV gene, agaw gene, agaA gene, agaS gene, agaY gene, agaB gene, agaC gene, agaD gene, agaI gene, mtr gene, deaD gene, nlpI gene, pnp gene, rpsO gene, truB gene, rbfA gene, infB gene, nusA gene, argG gene, secG gene, mrsA gene, folP gene, ftsH gene, ftsJ gene, greA gene, dacB gene, yhbZ gene, rpmA gene, rplU gene, ispB gene, nlp gene, murA gene, yrbI gene, yrbK gene, rpoN gene, ptsN gene, ptsO gene, mtgA gene, arcB gene, gltB gene, nanT gene, npl gene, nanR gene, dcuD gene, sspB gene, sspA gene, rpsI gene, rpIM gene, hhoA gene, hhoB gene, mdh gene, argR gene, tldD gene, rng gene, mreD gene, mreC gene, mreB gene, accB gene, accC gene, panF gene, prmA gene, fis gene, aroE gene, smg gene, smf gene, def gene, fmt gene, sun gene, trkA gene, mscL gene, zntR gene, rplQ gene, rpoA gene, rpsD gene, rpsK gene, rpsM gene, rpmJ gene, secY gene, rplO gene, rpmD gene, rpsE gene, rplR gene, rplF gene, rpsH gene, rpsN gene, rplE gene, rplX gene, rplN gene, rpsQ gene, rpmC gene, rpiP gene, rpsC gene, rplV gene, rpsS gene, rplB gene, rplW gene, rpiD gene, rpiC gene, rpsJ gene, tufA gene, fusA gene, rpsG gene, rpsL gene, fkpA gene, slyX gene, slyD gene, kefB gene, prkB gene, crp gene, argD gene, pabA gene, fic gene, ppiA gene, nirB gene, nirD gene, nirC gene, cysG gene, php gene, trpS gene, gph gene, rpe gene, dam gene, damX gene, aroB gene, aroK gene, hofQ gene, mrcA gene, pckA gene, envZ gene, ompR gene, greB gene, feoA gene, feoB gene, bioH gene, gntT gene, malQ gene, malP gene, malT gene, rtcA gene, rtcB gene, rtcR gene, glpR gene, glpG gene, glpE gene, glpD gene, glgP gene, glgA gene, glgC gene, glgX gene, glgB gene, asd gene, gntU gene, gntU gene, gntK gene, gntR gene, ggt gene, ugpQ gene, ugpC gene, ugpE gene, ugpA gene, ugpB gene, livF gene, livG gene, livM gene, livH gene, livK gene, livJ gene, rpoH gene, ftsX gene, ftsE gene, ftsY gene, atzN gene, nikA gene, nikB gene, nikC gene, nikD gene, nikE gene, pitA gene, uspB gene, uspA gene, priC gene, gor gene, arsR gene, arsB gene, arsC gene, slp gene, hdeB gene, hdeA gene, hdeD gene, gadA gene, treF gene, kdgK gene, dctA gene, dppF gene, dppD gene, dppC gene, dppB gene, dppA gene, tag gene, bisC gene, cspA gene, glyS gene, glyQ gene, xylB gene, xylA gene, xylF gene, xylG gene, xylH gene, xylR gene, bax gene, malS gene, avtA gene, lyx gene, sgbH gene, sgbU gene, sgbE gene, aldB gene, selB gene, selA gene, mtlA gene, mtlD gene, mtlR gene, lldP gene, lldR gene, lldD gene, cysE gene, gpsA gene, secB gene, grxC gene, tdh gene, kbl gene, htrL gene, rfaD gene, rfaF gene, rfaC gene, rfaL gene, rfaK gene, rfaZ gene, rfaY gene, rfaJ gene, rfaI gene, rfaB gene, rfaS gene, rfaP gene, rfaG gene, rfaQ gene, kdtA gene, kdtB gene, mutM gene, rpmG gene, rpmB gene, radC gene, dfp gene, dut gene, ttk gene, pyrE gene, rph gene, dinD gene, gmk gene, rpoZ gene, spoT gene, spoU gene, recG gene, gltS gene, nlpA gene, uhpT gene, uhpC gene, uhpB gene, uhpA gene, ilvN gene, ilvB gene, ivbL gene, emrD gene, ibpB gene, ibpA gene, gyrB gene, recF gene, dnaN gene, dnaA gene, rpmH gene, rnpA gene, yidC gene, thdF gene, tnaL gene, tnaA gene, tnaB gene, bglB gene, bglF gene, bglG gene, phoU gene, pstB gene, pstA gene, pstC gene, pstS gene, glmS gene, glmU gene, atpC gene, uncD gene, atpG gene, atpA gene, atpH gene, atpF gene, atpE gene, atpB gene, atpI gene, gidB gene, gidA gene, mioC gene, asnC gene, pssR gene, ilvL gene, ilvG gene, ilvM gene, ilvE gene, ilvD gene, ilvA gene, ilvY gene, ilvC gene, ppiC gene, rep gene, gppA gene, rhIB gene, trxA gene, rhoL gene, rho gene, rfe gene, wzzE gene, nfrC gene, rffD gene, rffG gene, rffH gene, rffA gene, wzxE gene, rffT gene, wecG gene, aslB gene, aslA gene, hemY gene, hemX gene, hemD gene, hemC gene, cyaA gene, cyaY gene, dapF gene, xerC gene, uvrD gene, corA gene, radD gene, pldA gene, recQ gene, pldB gene, metR gene, metE gene, udp gene, ubiE gene, aarF gene, tatC gene, tatD gene, rfaH gene, ubiD gene, ubiB gene, fadA gene, fadB gene, pepQ gene, trkH gene, trkH gene, hemG gene, mobB gene, mobA gene, dsbA gene, polA gene, yihA gene, hemN gene, ntrC gene, ntrB gene, glnA gene, fdhE gene, fdol gene, fdoH gene, fdoG gene, fdhD gene, sodA gene, kdgT gene, cpxA gene, cpxR gene, cpxP gene, pfkA gene, sbp gene, cdh gene, tpiA gene, fpr gene, glpX gene, glpK gene, glpF gene, menG gene, menA gene, hslU gene, hslV gene, ftsN gene, cytR gene, priA gene, rpmE gene, metJ gene, metB gene, metL gene, metF gene, katG gene, gldA gene, talC gene, ppc gene, argE gene, argC gene, argB gene, argH gene, oxyR gene, udhA gene, trmA gene, btuB gene, murI gene, murB gene, birA gene, coaA gene, tufB gene, secE gene, nusG gene, rplK gene, rplA gene, rplJ gene, rplL gene, rpoB gene, rpoC gene, htrC gene, thiH gene, thiG gene, thiS gene, thiF gene, thiE gene, thiC gene, rsd gene, hemE gene, nfi gene, hupA gene, hydH gene, hydG gene, purD gene, purH gene, metA gene, aceB gene, aceA gene, aceK gene, arp gene, icIR gene, metH gene, pepE gene, lysC gene, pgi gene, ubiC gene, ubiA gene, plsB gene, dgkA gene, lexA gene, dinF gene, qor gene, dnaB gene, alr gene, tyrB gene, napA gene, hobH gene, uvrA gene, ssb gene, soxS gene, soxR gene, acs gene, nrfA gene, nrfB gene, nrfC gene, nrfD gene, nrfE gene, nrfF gene, nrfG gene, gltP gene, fdhF gene, alsR gene, rpiB gene, proP gene, basS gene, basR gene, adiY gene, adi gene, melR gene, melA gene, melB gene, fumB gene, dcuB gene, dcuR gene, dcuS gene, lysU gene, cadA gene, cadB gene, cadC gene, cutA3 gene, cutA2 gene, cutA gene, dcuA gene, aspA gene, fxsA gene, groES gene, groEL gene, efp gene, ecnB gene, sugE gene, blc gene, ampC gene, frdD gene, frdC gene, frdB gene, frdA gene, genX gene, psd gene, yjeQ gene, yjeE gene, amiB gene, mutL gene, miaA gene, hfq gene, hflX gene, hflK gene, hflC gene, purA gene, vacB gene, sgaE gene, rpsF gene, priB gene, rpsR gene, rplI gene, fklB gene, cycA gene, cpdB gene, cysQ gene, msrA gene, chpS gene, chpB gene, ppa gene, fbp gene, mpl gene, pmbA gene, cybC gene, nrdG gene, nrdD gene, treC gene, treB gene, treR gene, mgtA gene, pyrI gene, pyrB gene, pyrBI gene, argl gene, valS gene, holC gene, xerB gene, idnR gene, idnT gene, idnO gene, idnD gene, idnK gene, fecE gene, fecD gene, fecC gene, fecB gene, fecA gene, fecR gene, fecI gene, dnaC gene, dnaT gene, bglJ gene, fhuF gene, rsmC gene, holD gene, rimI gene, prfC gene, osmY gene, deoC gene, deoA gene, deoB gene, deoD gene, lplA gene, smp gene, serB gene, radA gene, nadR gene, slt gene, trpR gene, gpmG gene, rob gene, creA gene, creB gene, creC gene, creD gene, arcA gene, lasT gene, thrL gene, spr gene, bcr gene, rsuA gene, or homologous gene thereof and comprising chromosomal DNA that is at least 933 kbp shorter than that of a wild-type E. coli strain.

24. The E. coli mutant strain according to claim 23 comprising chromosomal DNA that comprises DNA having the nucleotide sequences as shown in [1] to [55] below of SEQ ID NO: 1 or DNA equivalent thereto. [1] a nucleotide sequence of nucleotides 1 to 167064 [2] a nucleotide sequence of nucleotides 172980 to 243964 [3] a nucleotide sequence of nucleotides 253411 to 261963 [4] a nucleotide sequence of nucleotides 387532 to 389138 [5] a nucleotide sequence of nucleotides 403704 to 518029 [6] a nucleotide sequence of nucleotides 532712 to 535504 [7] a nucleotide sequence of nucleotides 550216 to 563940 [8] a nucleotide sequence of nucleotides 608119 to 675103 [9] a nucleotide sequence of nucleotides 689375 to 728820 [10] a nucleotide sequence of nucleotides 739594 to 832551 [11] a nucleotide sequence of nucleotides 848090 to 867637 [12] a nucleotide sequence of nucleotides 883474 to 890997 [13] a nucleotide sequence of nucleotides 909380 to 1048887 [14] a nucleotide sequence of nucleotides 1097020 to 1198105 [15] a nucleotide sequence of nucleotides 1225147 to 1630911 [16] a nucleotide sequence of nucleotides 1641039 to 1642631 [17] a nucleotide sequence of nucleotides 1650223 to 1964523 [18] a nucleotide sequence of nucleotides 1993076 to 1996661 [19] a nucleotide sequence of nucleotides 2028210 to 2033448 [20] a nucleotide sequence of nucleotides 2044377 to 2069148 [21] a nucleotide sequence of nucleotides 2081927 to 2104392 [22] a nucleotide sequence of nucleotides 2116065 to 2146263 [23] a nucleotide sequence of nucleotides 2187856 to 2200668 [24] a nucleotide sequence of nucleotides 2229851 to 2255894 [25] a nucleotide sequence of nucleotides 2263492 to 2270439 [26] a nucleotide sequence of nucleotides 2286589 to 2386261 [27] a nucleotide sequence of nucleotides 2395472 to 2423364 [28] a nucleotide sequence of nucleotides 2434099 to 2472851 [29] a nucleotide sequence of nucleotides 2482483 to 2505074 [30] a nucleotide sequence of nucleotides 2514558 to 2755676 [31] a nucleotide sequence of nucleotides 2789481 to 2795173 [32] a nucleotide sequence of nucleotides 2800891 to 2804307 [33] a nucleotide sequence of nucleotides 2810148 to 2825313 [34] a nucleotide sequence of nucleotides 2830263 to 2910273 [35] a nucleotide sequence of nucleotides 2910574 to 2985361 [36] a nucleotide sequence of nucleotides 3033129 to 3077084 [37] a nucleotide sequence of nucleotides 3078917 to 3110109 [38] a nucleotide sequence of nucleotides 3134342 to 3165627 [39] a nucleotide sequence of nucleotides 3172941 to 3360736 [40] a nucleotide sequence of nucleotides 3370548 to 3411159 [41] a nucleotide sequence of nucleotides 3420879 to 3453020 [42] a nucleotide sequence of nucleotides 3469367 to 3618575 [43] a nucleotide sequence of nucleotides 3636917 to 3854661 [44] a nucleotide sequence of nucleotides 3865832 to 3867101 [45] a nucleotide sequence of nucleotides 3877011 to 3926658 [46] a nucleotide sequence of nucleotides 3938879 to 4059897 [47] a nucleotide sequence of nucleotides 4079660 to 4086450 [48] a nucleotide sequence of nucleotides 4099983 to 4139231 [49] a nucleotide sequence of nucleotides 4149710 to 4235358 [50] a nucleotide sequence of nucleotides 4251736 to 4298976 [51] a nucleotide sequence of nucleotides 4311512 to 4313317 [52] a nucleotide sequence of nucleotides 4329722 to 4409532 [53] a nucleotide sequence of nucleotides 4423152 to 4496201 [54] a nucleotide sequence of nucleotides 4509535 to 4517875 [55] a nucleotide sequence of nucleotides 4599638 to 4641433 and DNA having a nucleotide sequence comprising nucleotides 2270440 to 2286588 of SEQ ID NO: 1 or DNA equivalent thereto.

25. The E. coli mutant strain according to claim 23 or 24 comprising chromosomal DNA that does not comprise yafJ gene, yafK gene, yafQ gene, dinJ gene, yafL gene, yafM gene, fhiA gene, lafU gene, dinP gene, yafN gene, yafO gene, yafP gene, JW0225 gene, prfH gene, ykfI gene, yafW gene, yeeT gene, ykfG gene, yafX gene, ykfF gene, ykfB gene, yafY gene, ypjK gene, yafZ gene, ykfA gene, perR gene, JW0245 gene, JW0246 gene, JW0247 gene, ykfC gene, JW0249 gene, is5B gene, yi51 gene, mmuP gene, mmuM gene, afuC gene, JW0255 gene, insB gene, insA gene, JW0258 gene, yagB gene, yagA gene, yagE gene, yagF gene, yagG gene, yagH gene, yagI gene, argF gene, yfjI gene, yagJ gene, yagK gene, yagL gene, yagM gene, yagN gene, intF gene, yagP gene, yagQ gene, yagR gene, yagS gene, yagT gene, yagU gene, ykgJ gene, yagV gene, yagW gene, yagX gene, yagY gene, yagZ gene, ykgK gene, ykgL gene, ykgM gene, eaeH gene, JW0292 gene, JW0293 gene, ykgA gene, ykgB gene, ykgI gene, merA gene, ykgD gene, ykgE gene, ykgF gene, ykgG gene, ykgH gene, betA gene, betB gene, betI gene, betT gene, yahA gene, yahB gene, yahC gene, yahD gene, yahE gene, yahF gene, yahG gene, yahH gene, arcM gene, yahJ gene, yahK gene, yahL gene, yahM gene, yahN gene, yahO gene, prpR gene, bcpA gene, prpC gene, prpD gene, prpE gene, codB gene, codA gene, cynR gene, cynT gene, cynS gene, cynX gene, lacA gene, lacY gene, lacZ gene, lacI gene, mhpR gene, mhpA gene, mhpB gene, mhpC gene, mhpD gene, mhpF gene, mhpE gene, mhpT gene, yaiL gene, yaiM gene, adhC gene, yaiN gene, yaiO gene, JW0350 gene, yi2A gene, yi22 gene, yi24 gene, yi23 gene, yaiP gene, yaiS gene, tauA gene, tauB gene, tauC gene, ssiD gene, yaiT gene, JW0363 gene, JW0364 gene, yaiU gene, yaiV gene, ampH gene, sbmA gene, yaiW gene, yaiY gene, yaiZ gene, ddlA gene, yaiB gene, phoA gene, psiF gene, yaiC gene, ybbO gene, tesA gene, ybbA gene, ybbP gene, rhsD gene, ybbC gene, rhsE gene, ybbD gene, JW0490 gene, ybbB gene, ybbS gene, ybbT gene, ybbU gene, ybbQ gene, ybbV gene, allP gene, allA gene, ybbY gene, ybbZ gene, ylbA gene, allC gene, ylbC gene, fdrA gene, ylbE gene, ylbE gene, ylbE gene, ylbF gene, arcC gene, intD gene, EXO_LAMBD gene, VRPP_LAMBD gene, JW0528 gene, JW0529 gene, VREN_LAMBD gene, emrE gene, ybcK gene, ybcL gene, ybcM gene, ybcN gene, ninE gene, ybcO gene, rus gene, ybcQ gene, JW0539.5 gene, nmpC gene, vlyS gene, ybcS gene, ybcT gene, vboR gene, ybcV gene, ybcW gene, nohB gene, ybcX gene, ybcY gene, JW0552 gene, appY gene, ompT gene, envY gene, ybcH gene, nfrA gene, nfrB gene, cusS gene, cusR gene, cusC gene, ylcC gene, cusB gene, cusA gene, pheP gene, ybdG gene, nfnB gene, ybdF gene, ybdJ gene, ybdK gene, hokE gene, yi81 gene, yi82 gene, ymcC gene, ymcD gene, cspH gene, cspG gene, sfa gene, yccL gene, yccM gene, torS gene, torT gene, torR gene, torC gene, torA gene, torD gene, yccD gene, cbpA gene, yccE gene, agp gene, yccJ gene, wrbA gene, yciG gene, ycdG gene, JW0992 gene, JW0993 gene, JW0994 gene, ycdK gene, JW0996 gene, JW0997 gene, ycdC gene, putA gene, yzpU gene, putP gene, JW1002 gene, ycdO gene, ycdB gene, phoH gene, ycdP gene, ycdQ gene, ycdR gene, ycdR gene, ycdS gene, ycdT gene, JW1012 gene, JW1013 gene, JW1014 gene, ycdU gene, ymfD gene, ymfE gene, lit gene, intE gene, VXIS_BPP21 gene, ymfH gene, ymfI gene, ymfJ gene, RPC2_BPP22 gene, JW1132 gene, ymfL gene, ymfM gene, ymfN gene, ymfR gene, ymfO gene, JW1138 gene, JW1139 gene, ycfK gene, ymfS gene, ycfA gene, ycfE gene, pin gene, mcrA gene, JW1146 gene, ycgW gene, ycgX gene, ycgE gene, JW1150 gene, ycgZ gene, ymgA gene, JW1153 gene, ymgC gene, yahA gene, JW1156 gene, JW1157 gene, aidA gene, ymgD gene, JW1160 gene, JW1161 gene, JW1162 gene, ydfHl gene, ydfZ gene, ydfI gene, shiA gene, JW1537 gene, tnpR gene, ydfM gene, Y314_LAMBD gene, nohA gene, ydfO gene, yccL gene, cspI gene, ydfR gene, vlyS gene, cspB gene, cspF gene, regQ_LAMBD gene, JW1552 gene, rem gene, hokD gene, relE gene, relB gene, ydfV gene, flxA gene, ydfW gene, ydfX gene, dicC gene, sdaC gene, yqeH gene, yqeI gene, yqeJ gene, yqeK gene, ygeF gene, ygeG gene, ygeH gene, ygeI gene, JW2822 gene, ygeK gene, JW2824 gene, JW2825 gene, JW2830 gene, ygeP gene, JW2832 gene, nlpD gene, yagR gene, ygeT gene, JW2836 gene, ygeV gene, ygeW gene, dpaL gene, ygeY gene, ygeZ gene, arcL gene, yqeB gene, JW2844 gene, ygfJ gene, gltD gene, JW2847 gene, ygfM gene, yagR gene, ygfO gene, ygfP gene, ygfQ gene, ygfR gene, ygfS gene, ygfT gene, pbuX gene, idi gene, epsM gene, exeL gene, exeC gene, JW2938 gene, JW2939 gene, JW2940 gene, JW2941 gene, yghK gene, glcB gene, glcG gene, glcF gene, glcD gene, glcC gene, JW2948 gene, yghQ gene, yghR gene, yghS gene, yghT gene, yihL gene, yihM gene, yihN gene, yshA gene, yihO gene, yihP gene, yihQ gene, yihR gene, yihS gene, yihT gene, yihU gene, yihV gene, yihW gene, yihX gene, rbn gene, yihZ gene, yiiD gene, yiiE gene, yiiF gene, yiiG gene, frvR gene, frvX gene, frvB gene, frvA gene, yiiL gene, rhaD gene, rhaA gene, rhaB gene, rhaS gene, rhaR gene, rhaT gene, ptsA gene, JW3920 gene, frwC gene, frwB gene, pflD gene, pflC gene, frwD gene, yijO gene, yijP gene, JW3986 gene, yjbF gene, yjbG gene, yjbH gene, JW3989.5 gene, yjbA gene, xylE gene, malG gene, malF gene, malE gene, malK gene, lamB gene, malM gene, yjbI gene, yjcP gene, yjcQ gene, yjcR gene, yjcS gene, alsK gene, alsE gene, alsC gene, alsA gene, alsB gene, phnQ gene, phnP gene, phnO gene, phnN gene, phnM gene, phnL gene, phnK gene, phnJ gene, phnI gene, phnH gene, phnG gene, phnF gene, phnE gene, phnD gene, phnC gene, phnB gene, phnA gene, yjdA gene, yjcZ gene, yidF gene, yidG gene, yidH gene, yidI gene, yidJ gene, yidK gene, yidL gene, JW3657 gene, glvG gene, glvB gene, glvC gene, yidP gene, yidE gene, yidQ gene, yidR gene, yidS gene, dgoT gene, dgoA gene, dgoK gene, dgoR gene, yidW gene, yidX gene, yidA gene, yidB gene, asnA gene, yieM gene, JW3724 gene, yieN gene, kup gene, rbsD gene, rbsA gene, rbsC gene, rbsB gene, rbsK gene, rbsR gene, yieO gene, yecT gene, flhE gene, flhA gene, flhB gene, cheZ gene, cheY gene, cheB gene, cheR gene, tap gene, tar gene, cheW gene, cheA gene, motB gene, motA gene, flhC gene, flhD gene, JW1882 gene, JW1883 gene, yecG gene, otsA gene, otsB gene, araH gene, araG gene, araF gene, yecI gene, yecJ gene, JW1892 gene, ftn gene, yecH gene, tyrP gene, uvrC gene, uvrY gene, yecF gene, sdiA gene, yecC gene, yecS gene, yedO gene, fliY gene, fliZ gene, fliA gene, fliC gene, fliD gene, fliS gene, fliT gene, amyA gene, yedD gene, yedE gene, yedF gene, yedK gene, yedL gene, yedN gene, yedM gene, fliE gene, fliF gene, fliG gene, fliH gene, fliI gene, fliJ gene, fliK gene, fliL gene, fliM gene, fliN gene, fliO gene, fliP gene, fliQ gene, fliR gene, rcsA gene, dsrB gene, JW1937 gene, yedA gene, vsr gene, dcm gene, yedJ gene, JW1946 gene, ompC gene, JW1948 gene, yedS gene, yedU gene, yedV gene, copR gene, yedX gene, JW1954 gene, JW1955 gene, JW1956 gene, JW1974 gene, JW1979 gene, JW1980 gene, yeeP gene, flu gene, JW1983 gene, yeeS gene, yeeT gene, yeeU gene, yeeV gene, yeeW gene, yefJ gene, JW2013 gene, yefJ gene, yefI gene, wbbJ gene, yefG gene, rfc gene, glf gene, rfbX gene, rfbC gene, rfbA gene, rfbD gene, rfbB gene, intA gene, yfjH gene, alpA gene, yfjI gene, JW2606 gene, yfjJ gene, yfjK gene, yfjL gene, yfjM gene, yfjN gene, yfjO gene, yfjP gene, yfjQ gene, yfjR gene, ypjK gene, yfjS gene, yfjT gene, yfjU gene, yfjV gene, JW2622 gene, yfjW gene, JW2623.5 gene, yfjX gene, yfjY gene, yfjZ gene, ypjF gene, ypjA gene, JW2629 gene, JW2630 gene, JW2631 gene, JW2632 gene, JW2633 gene, JW2634 gene, ygaE gene, yzzM gene, yqaE gene, JW2642 gene, ygaP gene, stpA gene, JW2645 gene, ygaC gene, ygaM gene, nrdH gene, nrdI gene, nrdE gene, proV gene, proW gene, proX gene, JW2655 gene, JW2656 gene, ygaZ gene, ygaH gene, srlA gene, srlB gene, srlD gene, gutM gene, srlR gene, gutQ gene, gltF gene, yhcA gene, yhcD gene, yhcE gene, yhcE gene, yhcF gene, yhcG gene, yhcH gene, nanK gene, nanE gene, yegE gene, alkA gene, yegD gene, JW2055 gene, JW2056 gene, JW2057 gene, JW2058 gene, yegM gene, yegN gene, yegO gene, yegB gene, baeS gene, baeR gene, JW2065 gene, yegQ gene, ogrK gene, JW2068 gene, JW2069 gene, yegS gene, gatR gene, JW2072 gene, JW2073 gene, gatR gene, gatD gene, gatC gene, gatB gene, gatA gene, gatZ gene, gatY gene, fbaB gene, xegT gene, yegU gene, yegV gene, yegW gene, yegX gene, molR gene, yehI gene, yehK gene, yehL gene, yehO gene, yehP gene, yehQ gene, yehR gene, yehS gene, yehT gene, yehU gene, yehV gene, yehW gene, yehX gene, yehY gene, yehZ gene, bglX gene, dld gene, pbpG gene, yohC gene, yeiI gene, yeiJ gene, yeiK gene, yeiL gene, yeiM gene, yeiN gene, yeiC gene, cmtA gene, cmtB gene, intB gene, JW4228 gene, yjgW gene, yjgX gene, yjgY gene, yjgZ gene, yi41 gene, yi42 gene, yjhB gene, yjhC gene, yjhD gene, yjhE gene, yi91 gene, JW4244 gene, JW4245 gene, JW4246 gene, yjhU gene, yjhF gene, yjhG gene, yjhH gene, yjhI gene, sgcR gene, sgcE gene, sgcA gene, sgcQ gene, sgcC gene, JW4266.5 gene, sgcX gene, yjhP gene, yjhQ gene, JW4269.5 gene, JW4270 gene, JW4270.5 gene, yjhR gene, yjhS gene, yjhT gene, yjhA gene, fimB gene, fimE gene, fimA gene, fimI gene, fimC gene, fimD gene, fimF gene, fimG gene, fimH gene, gntP gene, uxuA gene, uxuB gene, uxuR gene, yjiC gene, yjiD gene, yjiE gene, iadA gene, yjiG gene, yjiH gene, yjiI gene, yjiJ gene, yjiK gene, JW4296.5 gene, yjiL gene, yjiM gene, yjiN gene, yjiO gene, yjiP gene, yjiQ gene, yjiR gene, yjiS gene, yjiT gene, yjiV gene, mcrD gene, mcrC gene, mcrB gene, yjiW gene, hsdS gene, hsdM gene, hsdR gene, mrr gene, yjiA gene, yjiX gene, yjiY gene, tsr gene, yjjL gene, JW4320 gene, yjjM gene, yjjN gene, mdoB gene, yjjA gene, kdpF gene, ybfA gene, rhsC gene, JW0690 gene, ybfb gene, rhsA gene, ybfC gene, ybfL gene, ybfD gene, ybgA gene, yjfI gene, yjfJ gene, yjfK gene, yjfL gene, yjfM gene, yjfC gene, aidB gene, yjfN gene, yjfO gene, yjfP gene, yjfQ gene, yjfR gene, sgaT gene, sgaB gene, sgaA gene, sgaH gene, sgaU gene, rhsB gene, JW3448 gene, yhhH gene, yibA gene, yhhI gene, yhhJ gene, yhiH gene, yhiI gene, yhiJ gene, yhiK gene, yhiL gene, yhiM gene, yhiN gene, JW0782 gene, ybiA gene, dinG gene, ybiB gene, ybiC gene, ybiJ gene, ybiI gene, ybiX gene, ybiL gene, ybiM gene, ybiN gene, ybiO gene, glnQ gene, glnP gene, glnH gene, yliA gene, yliB gene, yliC gene, yliD gene, JWO817 gene, JWO818 gene, yliG gene, yliH gene, yliI gene, yliJ gene, dacC gene, deoR gene, ybjG gene, ybjC gene, mdaA gene, rimK gene, ybjN gene, potF gene, potG gene, potH gene, potI gene, ybjO gene, ybjF gene, artJ gene, artM gene, artQ gene, artI gene, artP gene, ybjP gene, JW0850 gene, JW0851 gene, JW0852 gene, ybjT gene and ltaA gene, or homologous gene thereof.

26. The E. coli mutant strain according to claim 25 comprising chromosomal DNA that does not comprise DNA having the nucleotide sequences as shown in [1] to [43] of SEQ ID NO: 1 or DNA equivalent thereto. [1] a nucleotide sequence of nucleotides 243965 to 253410 [2] a nucleotide sequence of nucleotides 261964 to 387531 [3] a nucleotide sequence of nucleotides 389139 to 403703 [4] a nucleotide sequence of nucleotides 518030 to 532711 [5] a nucleotide sequence of nucleotides 535505 to 550215 [6] a nucleotide sequence of nucleotides 563941 to 608118 [7] a nucleotide sequence of nucleotides 728821 to 739593 [8] a nucleotide sequence of nucleotides 832552 to 848089 [9] a nucleotide sequence of nucleotides 867638 to 883473 [10] a nucleotide sequence of nucleotides 890998 to 909379 [11] a nucleotide sequence of nucleotides 1048888 to 1097019 [12] a nucleotide sequence of nucleotides 1198106 to 1225146 [13] a nucleotide sequence of nucleotides 1630912 to 1641038 [14] a nucleotide sequence of nucleotides 1642632 to 1650222 [15] a nucleotide sequence of nucleotides 1964524 to 1993075 [16] a nucleotide sequence of nucleotides 1996662 to 2028209 [17] a nucleotide sequence of nucleotides 2033449 to 2044376 [18] a nucleotide sequence of nucleotides 2069149 to 2081926 [19] a nucleotide sequence of nucleotides 2104393 to 2116064 [20] a nucleotide sequence of nucleotides 2146264 to 2187855 [21] a nucleotide sequence of nucleotides 2200669 to 2229850 [22] a nucleotide sequence of nucleotides 2255895 to 2263491 [23] a nucleotide sequence of nucleotides 2755677 to 2789480 [24] a nucleotide sequence of nucleotides 2795174 to 2800890 [25] a nucleotide sequence of nucleotides 2804308 to 2810147 [26] a nucleotide sequence of nucleotides 2825314 to 2830262 [27] a nucleotide sequence of nucleotides 2985362 to 3033128 [28] a nucleotide sequence of nucleotides 3077085 to 3078916 [29] a nucleotide sequence of nucleotides 3110110 to 3134341 [30] a nucleotide sequence of nucleotides 3360737 to 3370547 [31] a nucleotide sequence of nucleotides 3618576 to 3636916 [32] a nucleotide sequence of nucleotides 3854662 to 3865831 [33] a nucleotide sequence of nucleotides 3867102 to 3877010 [34] a nucleotide sequence of nucleotides 3926659 to 3938878 [35] a nucleotide sequence of nucleotides 4059898 to 4079659 [36] a nucleotide sequence of nucleotides 4086451 to 4099982 [37] a nucleotide sequence of nucleotides 4139232 to 4149709 [38] a nucleotide sequence of nucleotides 4235359 to 4251735 [39] a nucleotide sequence of nucleotides 4298977 to 4311511 [40] a nucleotide sequence of nucleotides 4313318 to 4329721 [41] a nucleotide sequence of nucleotides 4409533 to 4423151 [42] a nucleotide sequence of nucleotides 4496202 to 4509534 [43] a nucleotide sequence of nucleotides 4517876 to 4599637

27. The E. coli mutant strain according to claim 26 comprising chromosomal DNA that does not comprise ygiS gene, ygiT gene, ygiU gene, JW2991 gene, ygiW gene, ygiX gene, ygiY gene, ygiZ gene, mdaB gene and ygiN gene, or homologous gene thereof.

28. The E. coli mutant strain according to claim 27 comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 3165628 to 3172940 of SEQ ID NO: 1 or DNA equivalent thereto.

29. The E. coli mutant strain according to claim 28 comprising chromosomal DNA that does not comprise ybeL gene, ybeQ gene, ybeR gene, ybeS gene, ybeT gene, ybeU gene, ybeV gene, ybew gene, ybeK gene, gltL gene, gltK gene, gltJ gene, ybeJ gene and JW0651 gene, or homologous gene thereof.

30. The E. coli mutant strain according to claim 29 comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 675104 to 689374 of SEQ ID NO: 1 or DNA equivalent thereto.

31. The E. coli mutant strain according to claim 30 comprising chromosomal DNA that does not comprise yfcC gene, JW2296 gene, yfcE gene, yfcF gene, yfcG gene, folX gene, JW2301 gene, yfcI gene, hisP gene, hisM gene, hisQ gene, hisJ gene, argT gene, yfdB gene, JW2346 gene, yfdH gene, JW2348 gene, yfdK gene, yfdL gene, yfdM gene, xylU gene, yfdN gene, yfdO gene, JW2356 gene, JW2357 gene, JW2358 gene, JW2359 gene, JW2360 gene, JW2378 gene, yqhT gene, ptwC gene and ptwB gene, or homologous gene thereof.

32. The E. coli mutant strain according to claim 31 comprising chromosomal DNA that does not comprise DNA having the nucleotide sequences as shown in [1] to [3] below of SEQ ID NO: 1 or DNA equivalent thereto. [1] a nucleotide sequence of nucleotides 2423365 to 2434098 [2] a nucleotide sequence of nucleotides 2472852 to 2482482 [3] a nucleotide sequence of nucleotides 2505075 to 2514557

33. The E. coli mutant strain according to claim 32 comprising chromosomal DNA that does not comprise pinO gene, gspA gene, gspC gene, gspD gene, gspE gene, hofF gene, hofG gene, hofH gene, gspI gene, gspJ gene, gspK gene, gspL gene, pshM gene, hofD gene, bfr gene, yheA gene and chiA gene, or homologous gene thereof.

34. The E. coli mutant strain according to claim 33 comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 3453021 to 3469366 of SEQ ID NO: 1 or DNA equivalent thereto.

35. The E. coli mutant strain according to claim 34 comprising chromosomal DNA that does not comprise elaB gene, elaA gene, elaC gene, elaD gene, yfbK gene, yfbL gene, yfbM gene, yfbN gene, JW2269 gene and JW2270 gene, or homologous gene thereof.

36. The E. coli mutant strain according to claim 35 comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 2386262 to 2395471 of SEQ ID NO: 1 or DNA equivalent thereto.

37. The E. coli mutant strain according to claim 36 comprising chromosomal DNA that does not comprise yhdJ gene, yhdU gene, envR gene, acrE gene, envD gene, yhdV gene, yhdW gene, yhdX gene and yhdY gene, or homologous gene thereof.

38. The E. coli mutant strain according to claim 37 comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 3411160 to 3420878 of SEQ ID NO: 1 or DNA equivalent thereto.

39. The E. coli mutant strain according to claim 38 comprising chromosomal DNA that does not comprise chpA gene or homologous gene thereof.

40. The E. coli mutant strain according to claim 39 comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 2910274 to 2910573 of SEQ ID NO: 1 or DNA equivalent thereto.

41. The E. coli mutant strain according to claim 40 comprising chromosomal DNA that does not comprise fhuA gene, fhuC gene, fhuD gene fhuB gene, or homologous gene thereof.

42. The E. coli mutant strain according to claim 41 comprising chromosomal DNA that does not comprise DNA having a nucleotide sequence comprising nucleotides 167065 to 172979 of SEQ ID NO: 1 or DNA equivalent thereto.

43. The E. coli mutant strain according to claim 42 obtained via modification by a method selected from among the following [1] to [5]: [1] a method for reducing or removing at least one mechanism for regulating biosynthesis of the useful substance; [2] a method for increasing the expression level of at least one enzyme associated with biosynthesis of the useful substance; [3] a method for increasing the copy number of at least one enzyme gene associated with biosynthesis of the useful substance; [4] a method for attenuating or blocking at least one metabolic pathway which branches from the biosynthetic pathway of the useful substance into metabolites other than the useful substance; or [5] a method for selecting a strain that exhibits higher resistance to an analogue of a useful substance than a wild-type E. coli strain.

44. The E. coli mutant strain according to claim 43, wherein the E. coli strain is the E. coli K-12, B, or W strain.

45. The E. coli mutant strain according to claim 44, wherein the E. coli K-12 strain is the E. coli W3110 strain (ATCC27325) or the E. coli MG1655 strain (ATCC47076).

46. A method for producing a useful substance comprising culturing an E. coli mutant strain according to claim 45 in a medium so as to generate and accumulate such useful substance in the culture and recovering the useful substance from the culture.

47. The method according to claim 46, wherein the useful substance is selected from the group consisting of proteins, peptides, amino acids, nucleic acids, vitamins, saccharides, organic acids, and lipids.