Claims
- 1. A method for identifying a chemical as a potential medicament for increasing gene expression, comprising the steps of:
- a) expressing a nucleic acid encoding an I.kappa.B protein having the sequence set forth as SEQ ID NO:18;
- b) preparing a complex comprising said I.kappa.B protein and an NF-.kappa.B protein and contacting the complex with said chemical; and
- c) identifying said chemical as a potential medicament for increasing gene expression by its capacity to dissociate the complex of step b).
- 2. The method according to claim 1 wherein said nucleic acid comprises nucleotides 95-1045 of SEQ ID NO:17.
- 3. The method according to claim 2 wherein said nucleic acid comprises the sequence set forth as SEQ ID NO:17.
- 4. A method for identifying a chemical as a potential medicament for decreasing gene expression, comprising the steps of:
- a) expressing a nucleic acid encoding an I.kappa.B protein having the sequence set forth as SEQ ID NO:18;
- b) preparing a complex comprising said I.kappa.B protein and an NF-.kappa.B protein and contacting the complex with said chemical; and
- c) identifying said chemical as a potential medicament for decreasing gene expression by its capacity to prevent or retard the dissociation of said I.kappa.B protein from the complex of step b).
- 5. The method according to claim 4 wherein said nucleic acid comprises nucleotides 95-1045 of SEQ ID NO:17.
- 6. The method according to claim 5 wherein said nucleic acid comprises the sequence set forth as SEQ ID NO:17.
Parent Case Info
The present application is a division of U.S. patent application Ser. No. 08/119,891, filed Sep. 10, 1993, now abandoned, which is a continuation of U.S. patent application Ser. No. 07/702,770, filed May 17, 1991, now abandoned.
US Referenced Citations (16)
Foreign Referenced Citations (1)
Number |
Date |
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0 196 864 |
Oct 1986 |
EPX |
Divisions (1)
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Number |
Date |
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Parent |
119891 |
Sep 1993 |
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Continuations (1)
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Number |
Date |
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Parent |
702770 |
May 1991 |
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