INHIBITORS OF GLUCOCORTICOID RECEPTOR

BACKGROUND

A need exists in the art for an effective treatment of cancer, neoplastic disease, and hypercortisolism.

BRIEF SUMMARY OF THE INVENTION

Provided herein are substituted steroidal derivative compounds and pharmaceutical compositions comprising said compounds. The subject compounds and compositions are useful as inhibitors of glucocorticoid receptors (GR). Futhermore, the subject compounds and compositions are useful for the treatment of cancer, such as prostate cancer, breast cancer, lung cancer, and ovarian cancer, and hypercortisolism.

Some embodiments provided herein describe compounds having the structure of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof:

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wherein

ring A is a heteroaryl, aryl, cycloalkyl, or heterocyclyl;
R¹is —NR^4aR^5a;
each R²is independently —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, halo, —OR⁶, —OH, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted hydroxyalkyl, —C(O)R⁶, —C(O)OR⁶, —C(O)NR⁴R⁵, —OC(O)OR⁶, —OC(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —S(O)R⁷, —SR⁷, —NR⁴S(O)₂NR⁴R⁵, —CN, —CO₂H, or —NO₂;
or R¹and R²on adjacent atoms are taken together with the atoms to which they are attached to form an optionally substituted heterocycle;
R³is optionally substituted C_2-8alkyl, halo, haloalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted heteroaryl, —Si(R⁶)₃, —OR⁶, or —S(O)₂R⁷;
R^4ais C_2-8alkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —S(O)₂R⁷, —C(O)N(R¹³)₂, —C(O)R⁶, or —C(O)OR⁶;
R^5ais —H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —S(O)₂R⁷, —C(O)N(R¹³)₂, —C(O)R⁶, or —C(O)OR⁶;
or R^4aand R^5aare taken together with the N atom to which they are attached to form an optionally substituted heterocycle;
R⁴and R⁵are each independently H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —S(O)₂R⁷, —C(O)N(R¹³)₂, —C(O)R⁶, or —C(O)OR⁶;
or R⁴and R⁵are taken together with the N atom to which they are attached to form a substituted or unsubstituted heterocycle;
each R⁶is independently optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, or optionally substituted heteroaryl;
R⁷is optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted aralkyl, optionally substituted heterocyclyl, or optionally substituted heteroaryl;
R⁸and R⁹are each independently —H, optionally substituted alkyl, haloalkyl, halo, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, —OH, —S(O)₂R⁷, —C(O)₂H, —C(O)R⁶, or —C(O)OR⁶;
or R⁸and R⁹are taken together with the atom to which they are attached to form a substituted or unsubstituted ring containing 0-2 heteroatoms selected from the group consisting of —O—, —NH—, —NR⁶—, —S—, and —S(O)₂—;
R¹⁰and R¹¹are each independently —H, optionally substituted alkyl, halo, haloalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, —OH, —S(O)₂R⁷, —C(O)₂H, —C(O)R⁶, or —C(O)OR⁶;
or R¹⁰and R¹¹are taken together with the atom to which they are attached to form a substituted or unsubstituted ring containing 0-2 heteroatoms selected from the group consisting of —O—, —NH—, —NR⁶—, —S—, and —S(O)₂—;
R¹²is hydrogen, optionally substituted alkyl, haloalkyl, hydroxy, halo, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, or optionally substituted heteroalkyl;
each R¹³is independently H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted aralkyl, optionally substituted heterocyclyl, or optionally substituted heteroaryl; and
n is 0, 1, 2, 3, or 4.

In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R¹²is hydrogen, alkyl, haloalkyl, hydroxy, halo, carbocyclyl, or heteroalkyl. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R¹²is C_1-6alkyl or hydrogen. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R¹²is methyl. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R¹²is H. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, ring A is monocyclic heteroaryl or monocyclic aryl. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, ring A is phenyl, pyridinyl, pyrimidinyl, pyrazinyl, or pyridazinyl. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, ring A is phenyl. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R^4ais C_2-8alkyl. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R^4ais C_3-6alkyl. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R^4ais C_2-4alkyl. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R^4ais ethyl, i-propyl, or t-butyl. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R^5ais —H, optionally substituted alkyl, or haloalkyl. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R^5ais —H or alkyl. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R^5ais C_1-6alkyl. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R^4aand R^5aare taken together with the N atom to which they are attached to form an optionally substituted heterocycle. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R^4aand R^5aare taken together with the N atom to which they are attached to form an optionally substituted pyrrolidinyl, an optionally substituted morpholinyl, an optionally substituted thiomorpholinyl, an optionally substituted piperidinyl, or an optionally substituted piperazinyl. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, n is 0, 1, or 2. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, n is 0. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, n is 1.

In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, each R²is independently —NR⁴R⁵, halo, —OR⁶, alkyl, fluoroalkyl, carbocyclyl, heteroalkyl, heterocyclyl, —S(O)₂NR⁴R⁵, or —S(O)₂R⁷. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R³is optionally substituted C_2-8alkyl, haloalkyl, or optionally substituted carbocyclyl. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R³is C_4-8alkyl, haloalkyl, or optionally substituted carbocyclyl. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R³is CF₃, t-butyl, or cyclopropyl. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R³is C_4-8alkyl. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R³is t-butyl. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R³is haloalkyl. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R³is CF₃. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R³is optionally substituted carbocyclyl. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R³is cyclopropyl. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R⁸and R⁹are each independently —H, alkyl, or carbocyclyl. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R⁸and R⁹are —H. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R¹⁰and R¹¹are each independently —H, C_1-6alkyl, halo, C_1-6alkoxy, or —OH. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R¹⁰and R¹¹are each —H. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R¹⁰and R¹¹are taken together with the atom to which they are attached to form a 3-, 4-, 5-, or 6-membered ring. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R⁴and R⁵are each independently —H, C_1-6alkyl, or —S(O)₂R⁷. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R⁴and R⁵attached to the same N atom are taken together with the N atom to which they are attached to form a substituted or unsubstituted 4-, 5-, or 6-membered ring heterocycle additionally containing 0-3 heteroatoms selected from the group consisting of —O—, —NH—, —NR⁶—, —S—, and —S(O)₂—. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R⁶is alkyl, carbocyclyl, or fluoroalkyl. In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, R⁷is alkyl, carbocyclyl, optionally substituted aryl, optionally substituted aralkyl, or optionally substituted heterocyclyl.

In some embodiments of a compound of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof, the compound has the structure of Formula (IIIa):

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Some embodiments provided herein describe a pharmaceutical composition comprising a pharmaceutically acceptable excipient and a compound of Formula (III) or (IIIa), or a pharmaceutically acceptable salt, solvate, or prodrug thereof.

Also provided herein are methods for treating or preventing cancer in a subject, the method comprising administering to the subject a therapeutically effective amount of a compound having the structure of Formula (III) or (IIIa), or a pharmaceutically acceptable salt, solvate, or prodrug thereof. Also provided herein are methods for reducing incidences of cancer recurrence, the method comprising administering to a subject in cancer remission a therapeutically effective amount of a compound having the structure of Formula (III) or (IIIa), or a pharmaceutically acceptable salt, solvate, or prodrug thereof. Some embodiments provided herein describe methods for treating a chemo-resistant cancer in a subject, the method comprising administering to the subject a therapeutically effective amount of a compound having the structure of Formula (III) or (IIIa), or a pharmaceutically acceptable salt, solvate, or prodrug thereof.

In some embodiments, the cancer is triple negative breast cancer, high grade serous ovarian cancer, castration resistant prostate cancer, or doubly resistant prostate cancer. In some embodiments, the cancer is non-small cell lung cancer. In some embodiments, the methods further comprise administering a second therapeutic agent to the subject. In some embodiments, the methods further comprise administering one or more additional therapeutic agents. In some embodiments, the second or additional therapeutic agent is an androgen receptor signaling inhibitor. In specific embodiments, the androgen receptor signaling inhibitor is 3,3′-diindolylmethane (DIM), abiraterone acetate, ARN-509, bexlosteride, bicalutamide, dutasteride, epristeride, enzalutamide, finasteride, flutamide, izonsteride, ketoconazole, N-butylbenzene-sulfonamide, nilutamide, megestrol, steroidal antiandrogens, turosteride, or any combinations thereof. In some embodiments, the second or additional therapeutic agent is a chemotherapeutic agent. In other embodiments, the second or additional therapeutic agent is cisplatin, carboplatin, paclitaxel, gemcitabine, doxorubicin, camptothecin, topotecan, or any combinations thereof. In some embodiments, the second or additional therapeutic agent is an immunotherapy agent (e.g., an anti-PD-L1 agent or an anti-PD1 agent). In certain embodiments, the second or additional therapeutic agent is an anti-PD-L1 agent. In certain embodiments, the second or additional therapeutic agent is an anti-PD1 agent.

Other embodiments provided herein describe methods for treating a hypercortisolism disease or disorder in a subject, the method comprising administering to the subject in need thereof a therapeutically effective amount of a compound having the structure of Formula (III) or (IIIa), or a pharmaceutically acceptable salt, solvate, or prodrug thereof. In certain embodiments, the hypercortisolism disease or disorder is Cushing's syndrome. In certain embodiments, the hypercortisolism disease or disorder is refractory Cushing's syndrome.

INCORPORATION BY REFERENCE

All publications, patents, and patent applications mentioned in this specification are herein incorporated by reference for the specific purposes identified herein.

DETAILED DESCRIPTION OF THE INVENTION

As used herein and in the appended claims, the singular forms “a,” “and,” and “the” include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to “an agent” includes a plurality of such agents, and reference to “the cell” includes reference to one or more cells (or to a plurality of cells) and equivalents thereof known to those skilled in the art, and so forth. When ranges are used herein for physical properties, such as molecular weight, or chemical properties, such as chemical formulae, all combinations and subcombinations of ranges and specific embodiments therein are intended to be included. The term “about” when referring to a number or a numerical range means that the number or numerical range referred to is an approximation within experimental variability (or within statistical experimental error), and thus the number or numerical range, in some instances, will vary between 1% and 15% of the stated number or numerical range. The term “comprising” (and related terms such as “comprise” or “comprises” or “having” or “including”) is not intended to exclude that in other certain embodiments, for example, an embodiment of any composition of matter, composition, method, or process, or the like, described herein, “consist of” or “consist essentially of” the described features. Definitions

As used in the specification and appended claims, unless specified to the contrary, the following terms have the meaning indicated below.

“Amino” refers to the —NH₂radical.

“Cyano” refers to the —CN radical.

“Nitro” refers to the —NO₂radical.

“Oxa” refers to the —O— radical.

“Oxo” refers to the ═O radical.

“Thioxo” refers to the ═S radical.

“Imino” refers to the ═N—H radical.

“Oximo” refers to the ═N—OH radical.

“Hydrazino” refers to the ═N—NH₂radical.

“Alkyl” refers to a straight or branched hydrocarbon chain radical consisting solely of carbon and hydrogen atoms, containing no unsaturation, having from one to fifteen carbon atoms (e.g., C₁-C₁₅alkyl). In certain embodiments, an alkyl comprises one to thirteen carbon atoms (e.g., C₁-C₁₃alkyl). In certain embodiments, an alkyl comprises one to eight carbon atoms (e.g., C₁-C₈alkyl). In other embodiments, an alkyl comprises one to five carbon atoms (e.g., C₁-C₅alkyl). In other embodiments, an alkyl comprises one to four carbon atoms (e.g., C₁-C₄alkyl). In other embodiments, an alkyl comprises one to three carbon atoms (e.g., C₁-C₃alkyl). In other embodiments, an alkyl comprises one to two carbon atoms (e.g., C₁-C₂alkyl). In other embodiments, an alkyl comprises one carbon atom (e.g., C₁alkyl). In other embodiments, an alkyl comprises five to fifteen carbon atoms (e.g., C₅-C₁₅alkyl). In other embodiments, an alkyl comprises five to eight carbon atoms (e.g., C₅-C₈alkyl). In other embodiments, an alkyl comprises two to five carbon atoms (e.g., C₂-C₅alkyl). In other embodiments, an alkyl comprises three to five carbon atoms (e.g., C₃-C₅alkyl). In other embodiments, the alkyl group is selected from methyl, ethyl, 1-propyl (n-propyl), 1-methylethyl (iso-propyl), 1-butyl (n-butyl), 1-methylpropyl (sec-butyl), 2-methylpropyl (iso-butyl), 1,1-dimethylethyl (tert-butyl), 1-pentyl (n-pentyl). The alkyl is attached to the rest of the molecule by a single bond. Unless stated otherwise specifically in the specification, an alkyl group is optionally substituted by one or more of the following substituents: halo, cyano, nitro, oxo, thioxo, imino, oximo, trimethylsilanyl, —OR^a, —SR^a, —OC(O)—R^a, —N(R^a)₂, —C(O)R^a, —C(O)OR^a, —C(O)N(R^a)₂, —N(R^a)C(O)OR^a, —OC(O)—N(R^a)₂, —N(R^a)C(O)R^a, —N(R^a)S(O)_tR^a(where t is 1 or 2), —S(O)_tOR^a(where t is 1 or 2), —S(O)_tR^a(where t is 1 or 2) and —S(O)_tN(R^a)₂(where t is 1 or 2) where each R^ais independently hydrogen, alkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), fluoroalkyl, carbocyclyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), carbocyclylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), aryl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), aralkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heterocyclyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heterocyclylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heteroaryl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), or heteroarylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl).

“Alkoxy” refers to a radical bonded through an oxygen atom of the formula —O-alkyl, where alkyl is an alkyl chain as defined above.

“Alkenyl” refers to a straight or branched hydrocarbon chain radical group consisting solely of carbon and hydrogen atoms, containing at least one carbon-carbon double bond, and having from two to twelve carbon atoms. In certain embodiments, an alkenyl comprises two to eight carbon atoms. In other embodiments, an alkenyl comprises two to four carbon atoms. The alkenyl is attached to the rest of the molecule by a single bond, for example, ethenyl (i.e., vinyl), prop-1-enyl (i.e., allyl), but-1-enyl, pent-1-enyl, penta-1,4-dienyl, and the like. Unless stated otherwise specifically in the specification, an alkenyl group is optionally substituted by one or more of the following substituents: halo, cyano, nitro, oxo, thioxo, imino, oximo, trimethylsilanyl, —OR^a, —SR^a, —OC(O)—R^a, —N(R^a)₂, —C(O)R^a, —C(O)OR^a, —C(O)N(R^a)₂, —N(R^a)C(O)OR^a, —OC(O)—N(R^a)₂, —N(R^a)C(O)R^a, —N(R^a)S(O)_tR^a(where t is 1 or 2), —S(O)_tOR^a(where t is 1 or 2), —S(O)_tR^a(where t is 1 or 2) and —S(O)_tN(R^a)₂(where t is 1 or 2) where each R^ais independently hydrogen, alkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), fluoroalkyl, carbocyclyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), carbocyclylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), aryl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), aralkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heterocyclyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heterocyclylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heteroaryl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), or heteroarylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl).

“Alkynyl” refers to a straight or branched hydrocarbon chain radical group consisting solely of carbon and hydrogen atoms, containing at least one carbon-carbon triple bond, having from two to twelve carbon atoms. In certain embodiments, an alkynyl comprises two to eight carbon atoms. In other embodiments, an alkynyl comprises two to six carbon atoms. In other embodiments, an alkynyl comprises two to four carbon atoms. The alkynyl is attached to the rest of the molecule by a single bond, for example, ethynyl, propynyl, butynyl, pentynyl, hexynyl, and the like. Unless stated otherwise specifically in the specification, an alkynyl group is optionally substituted by one or more of the following substituents: halo, cyano, nitro, oxo, thioxo, imino, oximo, trimethylsilanyl, —OR^a, —SR^a, —OC(O)—R^a, —N(R^a)₂, —C(O)R^a, —C(O)OR^a, —C(O)N(R^a)₂, —N(R^a)C(O)OR^a, —OC(O)—N(R^a)₂, —N(R^a)C(O)R^a, —N(R^a)S(O)_tR^a(where t is 1 or 2), —S(O)_tOR^a(where t is 1 or 2), —S(O)_tR^a(where t is 1 or 2) and —S(O)_tN(R^a)₂(where t is 1 or 2) where each R^ais independently hydrogen, alkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), fluoroalkyl, carbocyclyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), carbocyclylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), aryl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), aralkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heterocyclyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heterocyclylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heteroaryl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), or heteroarylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl).

“Alkylene” or “alkylene chain” refers to a straight or branched divalent hydrocarbon chain linking the rest of the molecule to a radical group, consisting solely of carbon and hydrogen, containing no unsaturation and having from one to twelve carbon atoms, for example, methylene, ethylene, propylene, n-butylene, and the like. The alkylene chain is attached to the rest of the molecule through a single bond and to the radical group through a single bond. The points of attachment of the alkylene chain to the rest of the molecule and to the radical group is through one carbon in the alkylene chain or through any two carbons within the chain In certain embodiments, an alkylene comprises one to eight carbon atoms (e.g., C₁-C₈alkylene). In other embodiments, an alkylene comprises one to five carbon atoms (e.g., C₁-C₅alkylene). In other embodiments, an alkylene comprises one to four carbon atoms (e.g., C₁-C₄alkylene). In other embodiments, an alkylene comprises one to three carbon atoms (e.g., C₁-C₃alkylene). In other embodiments, an alkylene comprises one to two carbon atoms (e.g., C₁-C₂alkylene). In other embodiments, an alkylene comprises one carbon atom (e.g., C₁alkylene). In other embodiments, an alkylene comprises five to eight carbon atoms (e.g., C₅-C₈alkylene). In other embodiments, an alkylene comprises two to five carbon atoms (e.g., C₂-C₅alkylene). In other embodiments, an alkylene comprises three to five carbon atoms (e.g., C₃-C₅alkylene). Unless stated otherwise specifically in the specification, an alkylene chain is optionally substituted by one or more of the following substituents: halo, cyano, nitro, oxo, thioxo, imino, oximo, trimethylsilanyl, —OR^a, —SR^a, —OC(O)—R^a, —N(R^a)₂, —C(O)R^a, —C(O)OR^a, —C(O)N(R^a)₂, —N(R^a)C(O)OR^a, —OC(O)—N(R^a)₂, —N(R^a)C(O)R^a, —N(R^a)S(O)_tR^a(where t is 1 or 2), —S(O)_tOR^a(where t is 1 or 2), —S(O)_tR^a(where t is 1 or 2) and —S(O)_tN(R^a)₂(where t is 1 or 2) where each R^ais independently hydrogen, alkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), fluoroalkyl, carbocyclyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), carbocyclylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), aryl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), aralkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heterocyclyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heterocyclylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heteroaryl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), or heteroarylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl).

“Alkynylene” or “alkynylene chain” refers to a straight or branched divalent hydrocarbon chain linking the rest of the molecule to a radical group, consisting solely of carbon and hydrogen, containing at least one carbon-carbon triple bond, and having from two to twelve carbon atoms. The alkynylene chain is attached to the rest of the molecule through a single bond and to the radical group through a single bond. In certain embodiments, an alkynylene comprises two to eight carbon atoms (e.g., C₂-C₈alkynylene). In other embodiments, an alkynylene comprises two to five carbon atoms (e.g., C₂-C₅alkynylene). In other embodiments, an alkynylene comprises two to four carbon atoms (e.g., C₂-C₄alkynylene). In other embodiments, an alkynylene comprises two to three carbon atoms (e.g., C₂-C₃alkynylene). In other embodiments, an alkynylene comprises two carbon atom (e.g., C₂alkylene). In other embodiments, an alkynylene comprises five to eight carbon atoms (e.g., C₅-C₈alkynylene). In other embodiments, an alkynylene comprises three to five carbon atoms (e.g., C₃-C₅alkynylene). Unless stated otherwise specifically in the specification, an alkynylene chain is optionally substituted by one or more of the following substituents: halo, cyano, nitro, oxo, thioxo, imino, oximo, trimethylsilanyl, —OR^a, —SR^a, —OC(O)—R^a, —N(R^a)₂, —C(O)R^a, —C(O)OR^a, —C(O)N(R^a)₂, —N(R^a)C(O)OR^a, —OC(O)—N(R^a)₂, —N(R^a)C(O)R^a, —N(R^a)S(O)_tR^a(where t is 1 or 2), —S(O)_tOR^a(where t is 1 or 2), —S(O)_tR^a(where t is 1 or 2) and —S(O)_tN(R^a)₂(where t is 1 or 2) where each R^ais independently hydrogen, alkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), fluoroalkyl, carbocyclyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), carbocyclylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), aryl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), aralkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heterocyclyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heterocyclylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heteroaryl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), or heteroarylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl).

“Aryl” refers to a radical derived from an aromatic monocyclic or multicyclic hydrocarbon ring system by removing a hydrogen atom from a ring carbon atom. The aromatic monocyclic or multicyclic hydrocarbon ring system contains only hydrogen and carbon from five to eighteen carbon atoms, where at least one of the rings in the ring system is fully unsaturated, i.e., it contains a cyclic, delocalized (4n+2) π-relectron system in accordance with the HUckel theory. The ring system from which aryl groups are derived include, but are not limited to, groups such as benzene, fluorene, indane, indene, tetralin and naphthalene. Unless stated otherwise specifically in the specification, the term “aryl” or the prefix “ar-” (such as in “aralkyl”) is meant to include aryl radicals optionally substituted by one or more substituents independently selected from alkyl, alkenyl, alkynyl, halo, fluoroalkyl, cyano, nitro, optionally substituted aryl, optionally substituted aralkyl, optionally substituted aralkenyl, optionally substituted aralkynyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted heteroaryl, optionally substituted heteroarylalkyl, —R^b—OR^a, —R^b—OC(O)—R^a, —R^b—OC(O)—OR^a, —R^b—OC(O)—N(R^a)₂, —R^b—N(R^a)₂, —R^b—C(O) R^a, —R^b—C(O)OR^a, —R^b—C(O)N(R^a)₂, —R^b—O—R^c—C(O)N(R^a)₂, —R^b—N(R^a)C(O)OR^a, —R^b—N(R^a)C(O)R^a, —R^b—N(R^a)S(O)_tR^a(where t is 1 or 2), —R^b—S(O)_tR^a(where t is 1 or 2), —R^b—S(O)_tOR^a(where t is 1 or 2) and —R^b—S(O)_tN(R^a)₂(where t is 1 or 2), where each R^ais independently hydrogen, alkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), fluoroalkyl, cycloalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), cycloalkylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), aryl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), aralkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heterocyclyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heterocyclylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heteroaryl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), or heteroarylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), each R^bis independently a direct bond or a straight or branched alkylene or alkenylene chain, and R^cis a straight or branched alkylene or alkenylene chain, and where each of the above substituents is unsubstituted unless otherwise indicated.

“Aralkyl” refers to a radical of the formula —R^c-aryl where R^cis an alkylene chain as defined above, for example, methylene, ethylene, and the like. The alkylene chain part of the aralkyl radical is optionally substituted as described above for an alkylene chain The aryl part of the aralkyl radical is optionally substituted as described above for an aryl group.

“Aralkenyl” refers to a radical of the formula —R^d-aryl where R^dis an alkenylene chain as defined above. The aryl part of the aralkenyl radical is optionally substituted as described above for an aryl group. The alkenylene chain part of the aralkenyl radical is optionally substituted as defined above for an alkenylene group.

“Aralkynyl” refers to a radical of the formula —R^e-aryl, where R^eis an alkynylene chain as defined above. The aryl part of the aralkynyl radical is optionally substituted as described above for an aryl group. The alkynylene chain part of the aralkynyl radical is optionally substituted as defined above for an alkynylene chain

“Aralkoxy” refers to a radical bonded through an oxygen atom of the formula —O—R^c-aryl where R^cis an alkylene chain as defined above, for example, methylene, ethylene, and the like. The alkylene chain part of the aralkyl radical is optionally substituted as described above for an alkylene chain. The aryl part of the aralkyl radical is optionally substituted as described above for an aryl group.

“Carbocyclyl” refers to a stable non-aromatic monocyclic or polycyclic hydrocarbon radical consisting solely of carbon and hydrogen atoms, which includes fused or bridged ring systems, having from three to fifteen carbon atoms. In certain embodiments, a carbocyclyl comprises three to ten carbon atoms. In other embodiments, a carbocyclyl comprises five to seven carbon atoms. The carbocyclyl is attached to the rest of the molecule by a single bond. Carbocyclyl is saturated (i.e., containing single C—C bonds only) or unsaturated (i.e., containing one or more double bonds or triple bonds). A fully saturated carbocyclyl radical is also referred to as “cycloalkyl.” Examples of monocyclic cycloalkyls include, e.g., cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and cyclooctyl. An unsaturated carbocyclyl is also referred to as “cycloalkenyl.” Examples of monocyclic cycloalkenyls include, e.g., cyclopentenyl, cyclohexenyl, cycloheptenyl, and cyclooctenyl. Polycyclic carbocyclyl radicals include, for example, adamantyl, norbornyl (i.e., bicyclo[2.2.1]heptanyl), norbornenyl, decalinyl, 7,7-dimethyl-bicyclo[2.2.1]heptanyl, and the like. Unless otherwise stated specifically in the specification, the term “carbocyclyl” is meant to include carbocyclyl radicals that are optionally substituted by one or more substituents independently selected from alkyl, alkenyl, alkynyl, halo, fluoroalkyl, oxo, thioxo, cyano, nitro, optionally substituted aryl, optionally substituted aralkyl, optionally substituted aralkenyl, optionally substituted aralkynyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted heteroaryl, optionally substituted heteroarylalkyl, —R^b—OR^a, —R^b—OC(O)—R^a, —R^b—OC(O)—OR^a, —R^b—OC(O)—N(R^a)₂, —R^b—N(R^a)₂, —R^b—C(O) R^a, —R^b—C(O)OR^a, —R^b—C(O)N(R^a)₂, —R^b—O—R^c—C(O)N(R^a)₂, —R^b—N(R^a)C(O)OR^a, —R^b—N(R^a)C(O)R^a, —R^b—N(R^a)S(O)_tR^a(where t is 1 or 2), —R^b—S(O)_tR^a(where t is 1 or 2), —R^b—S(O)_tOR^a(where t is 1 or 2) and —R^b—S(O)_tN(R^a)₂(where t is 1 or 2), where each R^ais independently hydrogen, alkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), fluoroalkyl, cycloalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), cycloalkylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), aryl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), aralkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heterocyclyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heterocyclylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heteroaryl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), or heteroarylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), each R^bis independently a direct bond or a straight or branched alkylene or alkenylene chain, and R^cis a straight or branched alkylene or alkenylene chain, and where each of the above substituents is unsubstituted unless otherwise indicated.

“Carbocyclylalkyl” refers to a radical of the formula —R^c-carbocyclyl where R^cis an alkylene chain as defined above. The alkylene chain and the carbocyclyl radical is optionally substituted as defined above.

“Carbocyclylalkynyl” refers to a radical of the formula —R^c-carbocyclyl where R^cis an alkynylene chain as defined above. The alkynylene chain and the carbocyclyl radical is optionally substituted as defined above.

“Carbocyclylalkoxy” refers to a radical bonded through an oxygen atom of the formula —O—R^c-carbocyclyl where R^cis an alkylene chain as defined above. The alkylene chain and the carbocyclyl radical is optionally substituted as defined above.

As used herein, “carboxylic acid bioisostere” refers to a functional group or moiety that exhibits similar physical, biological and/or chemical properties as a carboxylic acid moiety. Examples of carboxylic acid bioisosteres include, but are not limited to,

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and the like.

“Deuteroalkyl” refers to an alkyl group where 1 or more hydrogen atoms of an alkyl are replaced with deuterium.

“Halo” or “halogen” refers to bromo, chloro, fluoro or iodo substituents.

“Haloalkyl” refers to an alkyl radical, as defined above, that is substituted by one or more halo radicals, as defined above, for example, trifluoromethyl, difluoromethyl, fluoromethyl, 2,2,2-trifluoroethyl, 1-fluoromethyl-2-fluoroethyl, trichloromethyl, dichloromethyl, chloromethyl, 2,2,2-trichloroethyl, 1-chloromethyl-2-chloroethyl, tribromomethyl, dibromomethyl, bromomethyl, 2,2,2-tribromoethyl, 1-bromomethyl-2-bromoethyl, and the like. In some embodiments, the alkyl part of the haloalkyl radical is optionally substituted as defined above for an alkyl group.

“Fluoroalkyl” refers to an alkyl radical, as defined above, that is substituted by one or more fluoro radicals, as defined above, for example, trifluoromethyl, difluoromethyl, fluoromethyl, 2,2,2-trifluoroethyl, 1-fluoromethyl-2-fluoroethyl, and the like. In some embodiments, the alkyl part of the fluoroalkyl radical is optionally substituted as defined above for an alkyl group.

“Heteroalkyl” refers to an alkyl group in which one or more skeletal atoms of the alkyl are selected from an atom other than carbon, e.g., oxygen, nitrogen (e.g. —NH—, —N(alkyl)-), sulfur, or combinations thereof. A heteroalkyl is attached to the rest of the molecule at a carbon atom of the heteroalkyl. In one aspect, a heteroalkyl is a C₁-C₆heteroalkyl. Unless stated otherwise specifically in the specification, an heteroalkyl chain is optionally substituted by one or more of the following substituents: halo, cyano, nitro, oxo, thioxo, imino, oximo, trimethylsilanyl, —OR^a, —SR^a, —OC(O)—R^a, —N(R^a)₂, —C(O)R^a, —C(O)OR^a, —C(O)N(R^a)₂, —N(R^a)C(O)OR^a, —OC(O)—N(R^a)₂, —N(R^a)C(O)R^a, —N(R^a)S(O)_tR^a(where t is 1 or 2), —S(O)_tOR^a(where t is 1 or 2), —S(O)_tR^a(where t is 1 or 2) and —S(O)_tN(R^a)₂(where t is 1 or 2) where each R^ais independently hydrogen, alkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), fluoroalkyl, carbocyclyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), carbocyclylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), aryl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), aralkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heterocyclyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heterocyclylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heteroaryl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), or heteroarylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl).

“Heterocyclyl” refers to a stable 3- to 18-membered non-aromatic ring radical that comprises two to twelve carbon atoms and from one to six heteroatoms selected from nitrogen, oxygen and sulfur. Unless stated otherwise specifically in the specification, the heterocyclyl radical is a monocyclic, bicyclic, tricyclic or tetracyclic ring system, which optionally includes fused or bridged ring systems. The heteroatoms in the heterocyclyl radical are optionally oxidized. One or more nitrogen atoms, if present, are optionally quaternized. The heterocyclyl radical is partially or fully saturated. The heterocyclyl is attached to the rest of the molecule through any atom of the ring(s). Examples of such heterocyclyl radicals include, but are not limited to, dioxolanyl, thienyl[1,3]dithianyl, decahydroisoquinolyl, imidazolinyl, imidazolidinyl, isothiazolidinyl, isoxazolidinyl, morpholinyl, octahydroindolyl, octahydroisoindolyl, 2-oxopiperazinyl, 2-oxopiperidinyl, 2-oxopyrrolidinyl, oxazolidinyl, piperidinyl, piperazinyl, 4-piperidonyl, pyrrolidinyl, pyrazolidinyl, quinuclidinyl, thiazolidinyl, tetrahydrofuryl, trithianyl, tetrahydropyranyl, thiomorpholinyl, thiamorpholinyl, 1-oxo-thiomorpholinyl, and 1,1-dioxo-thiomorpholinyl. Unless stated otherwise specifically in the specification, the term “heterocyclyl” is meant to include heterocyclyl radicals as defined above that are optionally substituted by one or more substituents selected from alkyl, alkenyl, alkynyl, halo, fluoroalkyl, oxo, thioxo, cyano, nitro, optionally substituted aryl, optionally substituted aralkyl, optionally substituted aralkenyl, optionally substituted aralkynyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted heteroaryl, optionally substituted heteroarylalkyl, —R^b—OR^a, —R^b—OC(O)—R^a, —R^b—OC(O)—OR^a, —R^b—OC(O)—N(R^a)₂, —R^b—N(R^a)₂, —R^b—C(O) R^a, —R^b—C(O)OR^a, —R^b—C(O)N(R^a)₂, —R^b—O—R^c—C(O)N(R^a)₂, —R^b—N(R^a)C(O)OR^a, —R^b—N(R^a)C(O)R^a, —R^b—N(R^a)S(O)_tR^a(where t is 1 or 2), —R^b—S(O)_tR^a(where t is 1 or 2), —R^b—S(O)_tOR^a(where t is 1 or 2) and —R^b—S(O)_tN(R^a)₂(where t is 1 or 2), where each R^ais independently hydrogen, alkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), fluoroalkyl, cycloalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), cycloalkylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), aryl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), aralkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heterocyclyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heterocyclylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heteroaryl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), or heteroarylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), each R^bis independently a direct bond or a straight or branched alkylene or alkenylene chain, and R^cis a straight or branched alkylene or alkenylene chain, and where each of the above substituents is unsubstituted unless otherwise indicated.

“N-heterocyclyl” or “N-attached heterocyclyl” refers to a heterocyclyl radical as defined above containing at least one nitrogen and where the point of attachment of the heterocyclyl radical to the rest of the molecule is through a nitrogen atom in the heterocyclyl radical. An N-heterocyclyl radical is optionally substituted as described above for heterocyclyl radicals. Examples of such N-heterocyclyl radicals include, but are not limited to, 1-morpholinyl, 1-piperidinyl, 1-piperazinyl, 1-pyrrolidinyl, pyrazolidinyl, imidazolinyl, and imidazolidinyl.

“C-heterocyclyl” or “C-attached heterocyclyl” refers to a heterocyclyl radical as defined above containing at least one heteroatom and where the point of attachment of the heterocyclyl radical to the rest of the molecule is through a carbon atom in the heterocyclyl radical. A C-heterocyclyl radical is optionally substituted as described above for heterocyclyl radicals. Examples of such C-heterocyclyl radicals include, but are not limited to, 2-morpholinyl, 2- or 3- or 4-piperidinyl, 2-piperazinyl, 2- or 3-pyrrolidinyl, and the like.

“Heterocyclylalkyl” refers to a radical of the formula —R^c-heterocyclyl where R^cis an alkylene chain as defined above. If the heterocyclyl is a nitrogen-containing heterocyclyl, the heterocyclyl is optionally attached to the alkyl radical at the nitrogen atom. The alkylene chain of the heterocyclylalkyl radical is optionally substituted as defined above for an alkylene chain The heterocyclyl part of the heterocyclylalkyl radical is optionally substituted as defined above for a heterocyclyl group.

“Heterocyclylalkoxy” refers to a radical bonded through an oxygen atom of the formula —O—R^c-heterocyclyl where R^cis an alkylene chain as defined above. If the heterocyclyl is a nitrogen-containing heterocyclyl, the heterocyclyl is optionally attached to the alkyl radical at the nitrogen atom. The alkylene chain of the heterocyclylalkoxy radical is optionally substituted as defined above for an alkylene chain The heterocyclyl part of the heterocyclylalkoxy radical is optionally substituted as defined above for a heterocyclyl group.

“Heteroaryl” refers to a radical derived from a 3- to 18-membered aromatic ring radical that comprises two to seventeen carbon atoms and from one to six heteroatoms selected from nitrogen, oxygen and sulfur. As used herein, the heteroaryl radical is a monocyclic, bicyclic, tricyclic or tetracyclic ring system, wherein at least one of the rings in the ring system is fully unsaturated, i.e., it contains a cyclic, delocalized (4n+2) π-relectron system in accordance with the Hückel theory. Heteroaryl includes fused or bridged ring systems. The heteroatom(s) in the heteroaryl radical is optionally oxidized. One or more nitrogen atoms, if present, are optionally quaternized. The heteroaryl is attached to the rest of the molecule through any atom of the ring(s). Examples of heteroaryls include, but are not limited to, azepinyl, acridinyl, benzimidazolyl, benzindolyl, 1,3-benzodioxolyl, benzofuranyl, benzooxazolyl, benzo[d]thiazolyl, benzothiadiazolyl, benzo[b][1,4]dioxepinyl, benzo[b][1,4]oxazinyl, 1,4-benzodioxanyl, benzonaphthofuranyl, benzoxazolyl, benzodioxolyl, benzodioxinyl, benzopyranyl, benzopyranonyl, benzofuranyl, benzofuranonyl, benzothienyl (benzothiophenyl), benzothieno[3,2-d]pyrimidinyl, benzotriazolyl, benzo[4,6]imidazo[1,2-a]pyridinyl, carbazolyl, cinnolinyl, cyclopenta[d]pyrimidinyl, 6,7-dihydro-5H-cyclopenta[4,5]thieno[2,3-d]pyrimidinyl, 5,6-dihydrobenzo[h]quinazolinyl, 5,6-dihydrobenzo[h]cinnolinyl, 6,7-dihydro-5H-benzo[6,7]cyclohepta[1,2-c]pyridazinyl, dibenzofuranyl, dibenzothiophenyl, furanyl, furanonyl, furo[3,2-c]pyridinyl, 5,6,7,8,9,10-hexahydrocycloocta[d]pyrimidinyl, 5,6,7,8,9,10-hexahydrocycloocta[d]pyridazinyl, 5,6,7,8,9,10-hexahydrocycloocta[d]pyridinyl, isothiazolyl, imidazolyl, indazolyl, indolyl, indazolyl, isoindolyl, indolinyl, isoindolinyl, isoquinolyl, indolizinyl, isoxazolyl, 5,8-methano-5,6,7,8-tetrahydroquinazolinyl, naphthyridinyl, 1,6-naphthyridinonyl, oxadiazolyl, 2-oxoazepinyl, oxazolyl, oxiranyl, 5,6,6a,7,8,9,10,10a-octahydrobenzo[h]quinazolinyl, 1-phenyl-1H-pyrrolyl, phenazinyl, phenothiazinyl, phenoxazinyl, phthalazinyl, pteridinyl, purinyl, pyrrolyl, pyrazolyl, pyrazolo[3,4-d]pyrimidinyl, pyridinyl, pyrido[3,2-d]pyrimidinyl, pyrido[3,4-d]pyrimidinyl, pyrazinyl, pyrimidinyl, pyridazinyl, pyrrolyl, quinazolinyl, quinoxalinyl, quinolinyl, isoquinolinyl, tetrahydroquinolinyl, 5,6,7,8-tetrahydroquinazolinyl, 5,6,7,8-tetrahydrobenzo[4,5]thieno[2,3-d]pyrimidinyl, 6,7,8,9-tetrahydro-5H-cyclohepta[4,5]thieno[2,3-d]pyrimidinyl, 5,6,7,8-tetrahydropyrido[4,5-c]pyridazinyl, thiazolyl, thiadiazolyl, triazolyl, tetrazolyl, triazinyl, thieno[2,3-d]pyrimidinyl, thieno[3,2-d]pyrimidinyl, thieno[2,3-c]pyridinyl, and thiophenyl (i.e. thienyl). Unless stated otherwise specifically in the specification, the term “heteroaryl” is meant to include heteroaryl radicals as defined above which are optionally substituted by one or more substituents selected from alkyl, alkenyl, alkynyl, halo, fluoroalkyl, haloalkenyl, haloalkynyl, oxo, thioxo, cyano, nitro, optionally substituted aryl, optionally substituted aralkyl, optionally substituted aralkenyl, optionally substituted aralkynyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted heteroaryl, optionally substituted heteroarylalkyl, —R^b—OR^a, —R^b—OC(O)—R^a, —R^b—OC(O)—OR^a, —R^b—OC(O)—N(R^a)₂, —R^b—N(R^a)₂, —R^b—C(O) R^a, —R^b—C(O)OR^a, —R^b—C(O)N(R^a)₂, —R^b—O—R^c—C(O)N(R^a)₂, —R^b—N(R^a)C(O)OR^a, —R^b—N(R^a)C(O)R^a, —R^b—N(R^a)S(O)_tR^a(where t is 1 or 2), —R^b—S(O)_tR^a(where t is 1 or 2), —R^b—S(O)_tOR^a(where t is 1 or 2) and —R^b—S(O)_tN(R^a)₂(where t is 1 or 2), where each R^ais independently hydrogen, alkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), fluoroalkyl, cycloalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), cycloalkylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), aryl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), aralkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heterocyclyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heterocyclylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), heteroaryl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), or heteroarylalkyl (optionally substituted with halogen, hydroxy, methoxy, or trifluoromethyl), each R^bis independently a direct bond or a straight or branched alkylene or alkenylene chain, and R^cis a straight or branched alkylene or alkenylene chain, and where each of the above substituents is unsubstituted unless otherwise indicated.

“N-heteroaryl” refers to a heteroaryl radical as defined above containing at least one nitrogen and where the point of attachment of the heteroaryl radical to the rest of the molecule is through a nitrogen atom in the heteroaryl radical. An N-heteroaryl radical is optionally substituted as described above for heteroaryl radicals.

“C-heteroaryl” refers to a heteroaryl radical as defined above and where the point of attachment of the heteroaryl radical to the rest of the molecule is through a carbon atom in the heteroaryl radical. A C-heteroaryl radical is optionally substituted as described above for heteroaryl radicals.

“Heteroarylalkyl” refers to a radical of the formula —R^c-heteroaryl, where R^cis an alkylene chain as defined above. If the heteroaryl is a nitrogen-containing heteroaryl, the heteroaryl is optionally attached to the alkyl radical at the nitrogen atom. The alkylene chain of the heteroarylalkyl radical is optionally substituted as defined above for an alkylene chain. The heteroaryl part of the heteroarylalkyl radical is optionally substituted as defined above for a heteroaryl group.

“Heteroarylalkoxy” refers to a radical bonded through an oxygen atom of the formula —O—R^c-heteroaryl, where R^cis an alkylene chain as defined above. If the heteroaryl is a nitrogen-containing heteroaryl, the heteroaryl is optionally attached to the alkyl radical at the nitrogen atom. The alkylene chain of the heteroarylalkoxy radical is optionally substituted as defined above for an alkylene chain The heteroaryl part of the heteroarylalkoxy radical is optionally substituted as defined above for a heteroaryl group.

The compounds disclosed herein, in some embodiments, contain one or more asymmetric centers and thus give rise to enantiomers, diastereomers, and other stereoisomeric forms that are defined, in terms of absolute stereochemistry, as (R)— or (S)—. Unless stated otherwise, it is intended that all stereoisomeric forms of the compounds disclosed herein are contemplated by this disclosure. When the compounds described herein contain alkene double bonds, and unless specified otherwise, it is intended that this disclosure includes both E and Z geometric isomers (e.g., cis or trans.) Likewise, all possible isomers, as well as their racemic and optically pure forms, and all tautomeric forms are also intended to be included. The term “geometric isomer” refers to E or Z geometric isomers (e.g., cis or trans) of an alkene double bond. The term “positional isomer” refers to structural isomers around a central ring, such as ortho-, meta-, and para-isomers around a benzene ring.

The compounds described herein may exhibit their natural isotopic abundance, or one or more of the atoms may be artificially enriched in a particular isotope having the same atomic number, but an atomic mass or mass number different from the atomic mass or mass number predominantly found in nature. All isotopic variations of the compounds of the present invention, whether radioactive or not, are encompassed within the scope of the present invention. For example, hydrogen has three naturally occurring isotopes, denoted ¹H (protium), ²H (deuterium), and ³H (tritium). Protium is the most abundant isotope of hydrogen in nature. Enriching for deuterium may afford certain therapeutic advantages, such as increased in vivo half-life and/or exposure, or may provide a compound useful for investigating in vivo routes of drug elimination and metabolism. Isotopically-enriched compounds may be prepared by conventional techniques well known to those skilled in the art or by processes analogous to those described in the Schemes and Examples herein using appropriate isotopically-enriched reagents and/or intermediates. In some embodiments, the compounds described herein contain one or more isotopic variants (e.g., deuterium, tritium, ¹³C, and/or ¹⁴C).

A “tautomer” refers to a molecule wherein a proton shift from one atom of a molecule to another atom of the same molecule is possible. The compounds presented herein, in certain embodiments, exist as tautomers. In circumstances where tautomerization is possible, a chemical equilibrium of the tautomers will exist. The exact ratio of the tautomers depends on several factors, including physical state, temperature, solvent, and pH. Some examples of tautomeric equilibrium include:

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“Pharmaceutically acceptable salt” includes both acid and base addition salts. A pharmaceutically acceptable salt of any one of the substituted steroidal derivative compounds described herein is intended to encompass any and all pharmaceutically suitable salt forms. Preferred pharmaceutically acceptable salts of the compounds described herein are pharmaceutically acceptable acid addition salts and pharmaceutically acceptable base addition salts.

“Pharmaceutically acceptable acid addition salt” refers to those salts which retain the biological effectiveness and properties of the free bases, which are not biologically or otherwise undesirable, and which are formed with inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, hydroiodic acid, hydrofluoric acid, phosphorous acid, and the like. Also included are salts that are formed with organic acids such as aliphatic mono- and dicarboxylic acids, phenyl-substituted alkanoic acids, hydroxy alkanoic acids, alkanedioic acids, aromatic acids, aliphatic and. aromatic sulfonic acids, etc. and include, for example, acetic acid, trifluoroacetic acid, propionic acid, glycolic acid, pyruvic acid, oxalic acid, maleic acid, malonic acid, succinic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid, salicylic acid, and the like. Exemplary salts thus include sulfates, pyrosulfates, bisulfates, sulfites, bisulfites, nitrates, phosphates, monohydrogenphosphates, dihydrogenphosphates, metaphosphates, pyrophosphates, chlorides, bromides, iodides, acetates, trifluoroacetates, propionates, caprylates, isobutyrates, oxalates, malonates, succinate suberates, sebacates, fumarates, maleates, mandelates, benzoates, chlorobenzoates, methylbenzoates, dinitrobenzoates, phthalates, benzenesulfonates, toluenesulfonates, phenylacetates, citrates, lactates, malates, tartrates, methanesulfonates, and the like. Also contemplated are salts of amino acids, such as arginates, gluconates, and galacturonates (see, for example, Berge S.M. et al., “Pharmaceutical Salts,” Journal of Pharmaceutical Science, 66:1-19 (1997)). Acid addition salts of basic compounds are, in some embodiments, prepared by contacting the free base forms with a sufficient amount of the desired acid to produce the salt according to methods and techniques with which a skilled artisan is familiar.

“Pharmaceutically acceptable base addition salt” refers to those salts that retain the biological effectiveness and properties of the free acids, which are not biologically or otherwise undesirable. These salts are prepared from addition of an inorganic base or an organic base to the free acid Pharmaceutically acceptable base addition salts are, in some embodiments, formed with metals or amines, such as alkali and alkaline earth metals or organic amines Salts derived from inorganic bases include, but are not limited to, sodium, potassium, lithium, ammonium, calcium, magnesium, iron, zinc, copper, manganese, aluminum salts and the like. Salts derived from organic bases include, but are not limited to, salts of primary, secondary, and tertiary amines, substituted amines including naturally occurring substituted amines, cyclic amines and basic ion exchange resins, for example, isopropylamine, trimethylamine, diethylamine, triethylamine, tripropylamine, ethanolamine, diethanolamine, 2-dimethylaminoethanol, 2-diethylaminoethanol, dicyclohexylamine, lysine, arginine, histidine, caffeine, procaine, N,N-dibenzylethylenediamine, chloroprocaine, hydrabamine, choline, betaine, ethylenediamine, ethylenedianiline, N-methylglucamine, glucosamine, methylglucamine, theobromine, purines, piperazine, piperidine, N-ethylpiperidine, polyamine resins and the like. See Berge et al., supra.

As used herein, “treatment” or “treating,” or “palliating” or “ameliorating” are used interchangeably. These terms refer to an approach for obtaining beneficial or desired results including but not limited to therapeutic benefit and/or a prophylactic benefit. By “therapeutic benefit” is meant eradication or amelioration of the underlying disorder being treated. Also, a therapeutic benefit is achieved with the eradication or amelioration of one or more of the physiological symptoms associated with the underlying disorder such that an improvement is observed in the patient, notwithstanding that the patient is still afflicted with the underlying disorder. For prophylactic benefit, the compositions are, in some embodiments, administered to a patient at risk of developing a particular disease, or to a patient reporting one or more of the physiological symptoms of a disease, even though a diagnosis of this disease has not been made.

“Prodrug” is meant to indicate a compound that is, in some embodiments, converted under physiological conditions or by solvolysis to a biologically active compound described herein. Thus, the term “prodrug” refers to a precursor of a biologically active compound that is pharmaceutically acceptable. A prodrug is typically inactive when administered to a subject, but is converted in vivo to an active compound, for example, by hydrolysis. The prodrug compound often offers advantages of solubility, tissue compatibility or delayed release in a mammalian organism (see, e.g., Bundgard, H., Design of Prodrugs (1985), pp. 7-9, 21-24 (Elsevier, Amsterdam).

Abbreviations used herein have their conventional meaning within the chemical and biological arts. The following abbreviations have the indicated meaning throughout: Na₂HPO₄=disodium phosphate, AcOH=acetic acid, aq.=aqueous, NH4Cl=ammonium chloride, DCM=dichloromethane, DMPU=1,3-dimethyl-3,4,5,6-tetrahydro-2(1H)-pyrimidinone, ESI=electrospray ionization, EtOAc=ethyl acetate, g=gram, h=hour, LCMS=liquid chromatography mass spectrometry, LDA=lithium diisopropylamide, MgSO₄=magnesium sulfate, m/s=mass-to-charge ratio, mg=milligram, MeOH=methanol, min=minute, NMR =nuclear magnetic resonance, RT or rt=room temperature, sat.=saturated, NaHCO₃=sodium bicarbonate, NaBH₄=sodium borohydride, Na₂CO₃=sodium carbonate, NaCl=sodium chloride, Na₂SO₄=sodium sulfate, Na₂S₂O₃=sodium thiosulfate, TFA=trifluoroacetic acid, and THF=tetrahydrofuran.

Substituted Steroidal Derivative Compounds

Substituted steroidal derivative compounds are described herein that are GR inhibitors. These compounds, and compositions comprising these compounds, are useful for the treatment of cancer, neoplastic disease, and hypercortisolism diseases and disorders.

Some embodiments provided herein describe a compound having the structure of Formula (I), or a pharmaceutically acceptable salt, solvate, or prodrug thereof:

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wherein

ring A is a heteroaryl, aryl, cycloalkyl, or heterocyclyl;
R¹is —H, —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, halo, —OR⁶, —OH, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted hydroxyalkyl, optionally substituted aryl, optionally substituted heteroaryl, —C(O)OR⁶, —C(O)NR⁴R⁵, —OC(O)OR⁶, —OC(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —S(O)R⁷, —SR⁷, —NR⁴S(O)₂NR⁴R⁵, —P(O)(OR⁶)₂, —P(O)(R⁶)₂, —CN, —CO₂H, or —NO₂;
each R²is independently —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, halo, —OR⁶, —OH, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted hydroxyalkyl, —C(O)R⁶, —C(O)OR⁶, —C(O)NR⁴R⁵, —OC(O)OR⁶, —OC(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —S(O)R⁷, —SR⁷, —NR⁴S(O)₂NR⁴R⁵, —CN, —CO₂H, or —NO₂;
R³is optionally substituted alkyl, halo, haloalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted heteroaryl, —Si(R⁶)₃, —OR⁶, or —S(O)₂R⁷;
R⁴and R⁵are each independently —H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —S(O)₂R⁷, —C(O)N(R¹³)₂, —C(O)R⁶, or —C(O)OR⁶;
- or R⁴and R⁵attached to the same N atom are taken together with the N atom to which they are attached to form a substituted or unsubstituted heterocycle;
each R⁶is independently optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, or optionally substituted heteroaryl;
R⁷is optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted aralkyl, optionally substituted heterocyclyl, or optionally substituted heteroaryl;
R⁸and R⁹are each independently H, optionally substituted alkyl, haloalkyl, halo, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, —OH, —OR⁶, —NR⁴R⁵, —C(O)NR⁴R⁵, —CN, —S(O)₂R⁷, —C(O)₂H, —C(O)R⁶, or —C(O)OR⁶;
- or R⁸and R⁹are taken together with the atom to which they are attached to form a substituted or unsubstituted ring containing 0-2 heteroatoms selected from the group consisting of —O—, —NH—, —NR⁶—, —S—, and —S(O)₂—;
R¹⁰and R¹¹are each independently —H, optionally substituted alkyl, halo, haloalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, —OH, —S(O)₂R⁷, —C(O)₂H, —C(O)R⁶, or —C(O)OR⁶;
- or R¹⁰and R¹¹are taken together with the atom to which they are attached to form a substituted or unsubstituted ring containing 0-2 heteroatoms selected from the group consisting of —O—, —NH—, —NR⁶—, —S—, and —S(O)₂—;
R¹²is hydrogen, optionally substituted alkyl, haloalkyl, deuteroalkyl, hydroxy, halo, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, or optionally substituted heteroalkyl;
each R¹³is independently H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted aralkyl, optionally substituted heterocyclyl, or optionally substituted heteroaryl;
and n is 0, 1, 2, 3, or 4.

In some embodiments,

ring A is a monocyclic or bicyclic heteroaryl, monocyclic or bicyclic aryl, monocyclic cycloalkyl, or monocyclic heterocyclyl;
R¹is —H, —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, halo, —OR⁶, —OH, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted hydroxyalkyl, —C(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —NR⁴S(O)₂NR⁴R⁵, —P(O)(OR⁶)₂, —CN, or —CO₂H;
each R²is independently —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, halo, —OR⁶, —OH, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted hydroxyalkyl, —C(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —NR⁴S(O)₂NR⁴R⁵, —CN, or —CO₂H;
R³is optionally substituted alkyl, halo, haloalkyl, deuteroalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted heteroaryl, —Si(R⁶)₃, —OR⁶, or —S(O)₂R⁷;
R⁴and R⁵are each independently —H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —S(O)₂R⁷, —C(O)N(R¹³)₂, —C(O)R⁶, or —C(O)OR⁶;
- or R⁴and R⁵attached to the same N atom are taken together with the N atom to which they are attached to form a substituted or unsubstituted 4-, 5-, or 6-membered heterocycle;
R⁶is optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, or optionally substituted heteroaryl;
R⁷is optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted aralkyl, optionally substituted heterocyclyl, or optionally substituted heteroaryl;
R⁸and R⁹are each independently —H, optionally substituted alkyl, haloalkyl, halo, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, —OH, —S(O)₂R⁷, —C(O)₂H, —C(O)R⁶, or —C(O)OR⁶;
- or R⁸and R⁹are taken together with the atom to which they are attached to form a substituted or unsubstituted 3-, 4-, 5-, or 6-membered ring containing 0 or 1 heteroatom selected from the group consisting of —O—, —NH—, —NR⁶—, —S—, and —S(O)₂—;
R¹⁰and R¹¹are each independently —H, optionally substituted alkyl, halo, haloalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, or —OH;
- or R¹⁰and R¹¹are taken together with the atom to which they are attached to form a substituted or unsubstituted 3-, 4-, 5-, or 6-membered ring containing 0 or 1 heteroatom selected from the group consisting of —O—, —NH—, —NR⁶—, —S—, and —S(O)₂—;
R¹²is hydrogen, optionally substituted alkyl, haloalkyl, hydroxy, halo, optionally substituted carbocyclyl, or optionally substituted heteroalkyl;
each R¹³is independently H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted aralkyl, optionally substituted heterocyclyl, or optionally substituted heteroaryl;
and n is 0, 1, 2, 3, or 4.

In some embodiments,

ring A is aryl or bicyclic heteroaryl;
R¹is —NR⁴R⁵, halo, —OR⁶, C_1-6alkyl, C_1-6fluoroalkyl, C_3-6carbocyclyl, C_3-6heterocyclyl, aryl, heteroaryl, —C(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —NR⁴S(O)₂NR⁴R⁵, —P(O)(R⁶)₂, —P(O)(OR⁶)₂, or —CN;
each R²is independently —NR⁴R⁵, halo, —OR⁶, C_1-6alkyl, C_1-6fluoroalkyl, C_3-6carbocyclyl, C_1-6heteroalkyl, C_3-6heterocyclyl, or —CN;
R³is C_1-6alkyl, C_1-6fluoroalkyl, carbocyclyl, or heterocyclylalkyl;
R⁴and R⁵are each independently —H, C_1-6alkyl, C_1-6fluoroalkyl, C_3-6carbocyclyl, C_1-6carbocyclylalkyl, C_2-6heterocyclyl, C_1-6alkylC_3-6heterocyclyl, —S(O)₂R⁷, or —C(O)N(R¹³);
- or R⁴and R⁵attached to the same N atom are taken together with the N atom to which they are attached to form a substituted or unsubstituted 4-, 5-, or 6-membered heterocycle;
R⁶is alkyl;
R⁷is C_1-6alkyl, C_3-6carbocyclyl, or phenyl optionally substituted with halo or alkyl;
R⁸and R⁹are each independently —H, C_1-6alkyl, C_1-6fluoroalkyl, halo, or C_3-6carbocyclyl;
R¹⁰and R¹¹are each independently —H or C_1-6alkyl;
R¹²is hydrogen or C_1-6alkyl;
each R¹³is independently hydrogen or C_1-6alkyl;
and n is 0, 1, 2, 3, or 4.

In some embodiments,

ring A is phenyl, 3-benzodioxyxlyl, or 1,4-benzodioxanyl;
R¹is —NR⁴R⁵, halo, —OR⁶, alkyl, or fluoroalkyl;
each R²is independently —NR⁴R⁵, halo, alkyl, carbocyclyl, alkoxy, or —CN;
R³is C_1-6alkyl, C_1-6fluoroalkyl, carbocyclyl, deuteroalkyl, or heteroalkyl;
R⁴and R⁵are each independently —H, C_1-6alkyl, heteroalkyl, or —S(O)₂R⁷;
- or R⁴and R⁵attached to the same N atom are taken together with the N atom to which they are attached to form a substituted or unsubstituted 4-, 5-, or 6-membered heterocycle;
R⁶is alkyl;
R⁷is C_1-6alkyl, C_3-6carbocyclyl, or phenyl optionally substituted with halo or alkyl;
R⁸and R⁹are each independently —H or C_1-6alkyl;
R¹⁰and R¹¹are each independently —H;
R¹²is hydrogen;
each R¹³is independently hydrogen or C_1-6alkyl;
and n is 0, 1, or 2.

In some embodiments,

ring A is phenyl;
R¹is —NR⁴R⁵, —OR⁶, alkyl, or fluoroalkyl;
each R²is independently —NR⁴R⁵, halo, alkyl;
R³is C_1-6alkyl, C_1-6fluoroalkyl, carbocyclyl, deuteroalkyl, or heteroalkyl;
R⁴and R⁵are each independently —H, C_1-6alkyl, or heteroalkyl;
- or R⁴and R⁵attached to the same N atom are taken together with the N atom to which they are attached to form a substituted or unsubstituted 4-, 5-, or 6-membered heterocycle;
R⁶is alkyl;
R⁷is C_1-6alkyl, C_3-6carbocyclyl, or phenyl optionally substituted with halo or alkyl;
R⁸and R⁹are each independently —H or C_1-6alkyl;
R¹⁰and R¹¹are each independently —H;
R¹²is hydrogen;
each R¹¹is independently hydrogen or C_1-6alkyl;
and n is 0, 1, or 2.

In some embodiments,

ring A is 3-benzodioxyxlyl, or 1,4-benzodioxanyl;
R¹is —H;
R³is C_1-6alkyl;
R⁸and R⁹are each independently —H or C_1-6alkyl;
R¹⁰and R¹¹are each independently —H;
R¹²is hydrogen;
each R¹³is independently hydrogen or C_1-6alkyl;
and n is 0.

For any and all of the embodiments of Formula (I), substituents are selected from among a subset of the listed alternatives.

In some embodiments, ring A is monocyclic aryl, bicyclic aryl, monocyclic heteroaryl, or bicyclic heteroaryl. In some embodiments, ring A is monocyclic heteroaryl or monocyclic aryl. In some embodiments, ring A is bicyclic heteroaryl or bicyclic aryl. In some embodiments, ring A is N-bound heteroaryl. In some embodiments, ring A is C-bound heteroaryl. In some embodiments, ring A is aryl.

In certain embodiments, ring A is phenyl, naphthyl, furanyl, pyrrolyl, oxazolyl, thiazolyl, imidazolyl, pyrazolyl, triazolyl, tetrazolyl, isoxazolyl, isothiazolyl, oxadiazolyl, thiadiazolyl, pyridinyl, pyrimidinyl, pyrazinyl, pyridazinyl, triazinyl, quinolinyl, isoquinolinyl, quinazolinyl, quinoxalinyl, naphthyridinyl, indolyl, indazolyl, benzoxazolyl, benzisoxazolyl, benzofuranyl, benzothienyl, benzothiazolyl, benzimidazolyl, purinyl, cinnolinyl, phthalazinyl, pteridinyl, pyridopyrimidinyl, pyrazolopyrimidinyl, or azaindolyl. In some embodiments, R¹is furanyl, pyrrolyl, oxazolyl, thiazolyl, imidazolyl, pyrazolyl, triazolyl, tetrazolyl, isoxazolyl, isothiazolyl, oxadiazolyl, thiadiazolyl, pyridinyl, pyrimidinyl, pyrazinyl, pyridazinyl, or triazinyl. In some embodiments, ring A is pyrimidinyl, pyridinyl, pyrazinyl, triazinyl, or thiazolyl. In certain embodiments, ring A is C-bound imidazolyl. In certain embodiments, ring A is N-bound imidazolyl. In some embodiments, ring A is pyrimidinyl. In some embodiments, ring A is pyrimidinyl optionally substituted with alkyl or alkoxy. In some embodiments, ring A is pyrazinyl. In some embodiments, ring A is triazinyl. In some embodiments, ring A is thiazolyl.

In some embodiments, ring A is phenyl, pyridinyl, pyrimidinyl, pyrazinyl, or pyridazinyl. In some embodiments, ring A is phenyl.

In some embodiments, ring A is a bicyclic heteroaryl. In certain embodiments, ring A is an optionally substituted 3-benzodioxyxlyl or 1,4-benzodioxanyl. In some embodiments, ring A is an unsubstituted 3-benzodioxyxlyl or 1,4-benzodioxanyl. In some embodiments, ring A is phenyl, 3-benzodioxyxlyl, or 1,4-benzodioxanyl

In some embodiments, R¹is —H, —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, halo, optionally substituted alkyl, fluoroalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted hydroxyalkyl, optionally substituted aryl, optionally substituted heteroaryl, —C(O)NR⁴R⁵, —OC(O)OR⁶, —OC(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —S(O)R⁷, —SR⁷, —NR⁴S(O)₂NR⁴R⁵, —P(O)(OR⁶)₂, —P(O)(R⁶)₂, —CN, or —NO₂. In some embodiments, R¹is —H, —NR⁴R⁵, halo, —OR⁶, optionally substituted alkyl, fluoroalkyl, optionally substituted carbocyclyl, optionally substituted heterocyclyl, optionally substituted aryl, optionally substituted heteroaryl, —C(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —NR⁴S(O)₂NR⁴R⁵, —P(O)(R⁶)₂, —P(O)(OR⁶)₂, or —CN. In some embodiments, R¹is —H, —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, halo, —OR⁶, optionally substituted alkyl, fluoroalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, —C(O)R⁶, —C(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —NR⁴S(O)₂NR⁴R⁵, —CN, or —CO₂H. In some embodiments, R¹is —NR⁴R⁵, halo, —OR⁶, alkyl, fluoroalkyl, carbocyclyl, heteroalkyl, heterocyclyl, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —NR⁴S(O)₂NR⁴R⁵, or —CN. In some embodiments, R¹is —NR⁴R⁵, halo, —OR⁶, C_1-6alkyl, C_1-6fluoroalkyl, C_3-6carbocyclyl, C_3-6heterocyclyl, aryl, heteroaryl, —C(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —NR⁴S(O)₂NR⁴R⁵, —P(O)(R⁶)₂, —P(O)(OR⁶)₂, or —CN. In some embodiments, R¹is —NR⁴R⁵, halo, —OR⁶, —S(O)₂NR⁴R⁵, —P(O)(R⁶)₂, or —P(O)(OR⁶)₂. In certain embodiments, R¹is —NR⁴R⁵, halo, —OR⁶, alkyl, or fluoroalkyl. In certain embodiments, R¹is halo, —OR⁶, or alkyl. In other embodiments, R¹is —NMe₂, —NHMe, —NH₂, —NEt₂, —NHEt, —NPr₂, —NHPr, —N(i-Pr₂), —NH(i-Pr), —NBu₂, —NHBu, —NEt₂, —NHEt, —N(i-Bu₂), —NH(i-Bu), —NMeEt, —NMePr, —N(Me)(i-Pr), —NMePr, —N(Me)(Bu), —NMeBu, —NEtPr, —N(Et)(i-Pr), —N(Et)(Bu), —NEtBu, methyl, ethyl, propyl, iso-propyl, butyl, iso-butyl, sec-butyl, tert-butyl, methoxy, ethoxy, propoxy, iso-propoxy, morpholino, or pyrrolidino. In certain embodiments, R¹is C_1-6alkyl, fluoroalkyl, C_3-6carbocyclyl, or C_3-6heterocyclyl. In some embodiments, R¹is —H. In some embodiments, R¹is —NR⁴R⁵. In some embodiments, R¹is —NMe₂. In some embodiments, R¹is —NHMe. In some embodiments, R¹is —NH₂. In some embodiments, R¹is —NMeEt. In some embodiments, R¹is —NEt₂. In some embodiments, R¹is halo. In some embodiments, R¹is fluoro. In some embodiments, R¹is chloro. In some embodiments, R¹is bromo. In some embodiments, R¹is C_1-6alkyl. In some embodiments, R¹is methyl. In some embodiments, R¹is ethyl. In some embodiments, R¹is propyl. In some embodiments, R¹is iso-propyl. In some embodiments, R¹is carbocyclyl. In some embodiments, R¹is cyclopropyl. In some embodiments, R¹is cyclobutyl. In some embodiments, R¹is cyclopentyl. In some embodiments, R¹is cyclohexyl. In some embodiments, R¹is —OR⁶. In some embodiments, R¹is methoxy. In some embodiments, R¹is ethoxy. In some embodiments, R¹is propoxy. In some embodiments, R¹is iso-propoxy. In some embodiments, R¹is heterocyclyl. In some embodiments, R¹is azetadino, morpholino, thiomorpholino, piperidino, piperazino, or pyrrolidino. In some embodiments, R¹is morpholino. In some embodiments, R¹is pyrrolidino. In some embodiments, R¹is azetadino.

In other embodiments, R¹is optionally substituted monocyclic aryl, optionally substituted bicyclic aryl, optionally substituted monocyclic heteroaryl, or optionally substituted bicyclic heteroaryl. In certain embodiments, R¹is phenyl, naphthyl, furanyl, pyrrolyl, oxazolyl, thiazolyl, imidazolyl, pyrazolyl, triazolyl, tetrazolyl, isoxazolyl, isothiazolyl, oxadiazolyl, thiadiazolyl, pyridinyl, pyrimidinyl, pyrazinyl, pyridazinyl, triazinyl, quinolinyl, isoquinolinyl, quinazolinyl, quinoxalinyl, naphthyridinyl, indolyl, indazolyl, benzoxazolyl, benzisoxazolyl, benzofuranyl, benzothienyl, benzothiazolyl, benzimidazolyl, purinyl, cinnolinyl, phthalazinyl, pteridinyl, pyridopyrimidinyl, pyrazolopyrimidinyl, or azaindolyl. In some embodiments, R¹is furanyl, pyrrolyl, oxazolyl, thiazolyl, imidazolyl, pyrazolyl, triazolyl, tetrazolyl, isoxazolyl, isothiazolyl, oxadiazolyl, thiadiazolyl, pyridinyl, pyrimidinyl, pyrazinyl, pyridazinyl, or triazinyl. In some embodiments, R¹is pyrimidinyl, pyridinyl, pyrazinyl, triazinyl, or thiazolyl. In certain embodiments, R¹is C-bound imidazolyl. In certain embodiments, R¹is N-bound imidazolyl. In some embodiments, R¹is pyrimidinyl. In some embodiments, R¹is pyrimidinyl optionally substituted with alkyl or alkoxy. In some embodiments, R¹is pyrazinyl. In some embodiments, R¹is triazinyl. In some embodiments, R¹is thiazolyl.

In certain embodiments, R¹is optionally substituted with halo, alkyl, hydroxy, alkoxy, or fluoroalkyl. In certain embodiments, R¹is optionally substituted with halo, alkyl, or alkoxy. In some embodiments, R¹is optionally substituted with fluoro or C_1-6alkyl. In some embodiments, R¹is optionally substituted with fluoro or methyl. In some embodiments, R¹is optionally substituted with methyl or methoxy.

In some embodiments, each R²is independently —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, halo, —OR⁶, OH, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted hydroxyalkyl, —C(O)R⁶, —C(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —NR⁴S(O)₂NR⁴R⁵, or —CN. In some embodiments, each R²is independently —NR⁴R⁵, alkylNR⁴R⁵, halo, —OR⁶, —OH, alkyl, haloalkyl, carbocyclyl, carbocyclylalkyl, heteroalkyl, heterocyclyl, heterocyclylalkyl, hydroxyalkyl, —C(O)R⁶, —C(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —NR⁴S(O)₂NR⁴R⁵, or —CN. In some embodiments, —NR⁴R⁵e, halo, —OR⁶, optionally substituted alkyl, fluoroalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, —C(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —NR⁴S(O)₂NR⁴R⁵, —CN, or —CO₂H. In certain embodiments, each R²is independently —NR⁴R⁵, halo, —OR⁶, —OH, optionally substituted alkyl, fluoroalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted hydroxyalkyl, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —NR⁴S(O)₂NR⁴R⁵, or —CN. In some embodiments, each R²is independently —NR⁴R⁵, halo, —OR⁶, alkyl, fluoroalkyl, carbocyclyl, heteroalkyl, heterocyclyl, —S(O)₂NR⁴R⁵, —NR⁴S(O)₂NR⁴R⁵, or —S(O)₂R⁷. In some embodiments, each R²is independently —NR⁴R⁵, C_1-6alkylNR⁴R⁵, halo, —OR⁶, —OH, C_1-6alkyl, C_1-6fluoroalkyl, C_3-6carbocyclyl, C_1-6alkylC_3-6carbocyclyl, C_1-6heteroalkyl, C_3-6heterocyclyl, C_1-6alkylC_3-6heterocyclyl, C_1-6hydroxyalkyl, or —CN. In some embodiments, each R²is independently —C(O)R⁶, —C(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, or —CN. In some embodiments, each R²is independently —NR⁴R⁵, halo, —OR⁶, C_1-6alkyl, C_1-6fluoroalkyl, C_3-6carbocyclyl, C_1-6heteroalkyl, C_3-6heterocyclyl, or —CN. In some embodiments, each R²is independently —NR⁴R⁵, halo, alkyl, carbocyclyl, alkoxy, or —CN. In some embodiments, each R²is independently —NR⁴R⁵, halo, alkyl, or alkoxy. In some embodiments, each R²is independently methyl, methoxy, ethyl, propyl, iso-propyl, cyclopropyl, fluoro, chloro, or —NMe₂. In some embodiments, each R²is —NMe₂, —NHMe, —NH₂, —NEt₂, —NHEt, —NPr₂, —NHPr, —N(i-Pr₂), —NH(i-Pr), —NBu₂, —NHBu, —NEt₂, —NHEt, —N(i-Bu₂), —NH(i-Bu), —NMeEt, —NMePr, —N(Me)(i-Pr), —NMePr, —N(Me)(Bu), —NMeBu, —NEtPr, —N(Et)(i-Pr), —N(Et)(Bu), —NEtBu, methyl, ethyl, propyl, iso-propyl, butyl, iso-butyl, sec-butyl, tert-butyl, methoxy, ethoxy, propoxy, iso-propoxy, morpholino, or pyrrolidino. In some embodiments, each R²is independently methyl, methoxy, iso-propyl, cyclopropyl, fluoro, chloro, or —NMe₂. In some embodiments, R²is methoxy. In some embodiments, R²is methyl. In some embodiments R²is ethyl. In some embodiments, R²is iso-propyl. In some embodiments, R²is propyl. In some embodiments, R²is cyclopropyl. In some embodiments, R²is cyclobutyl. In some embodiments, R²is fluoro. In some embodiments, R²is chloro. In some embodiments, R²is —NMe₂. In some embodiments, R²is —NH₂. In some embodiments, R²is —NHMe. In some embodiments, R²is —NMeEt.

In certain embodiments, each R²is independently optionally substituted with halo, alkyl, hydroxy, alkoxy, or fluoroalkyl. In some embodiments, each R²is independently optionally substituted with fluoro or C_1-6alkyl. In some embodiments, each R²is independently optionally substituted with fluoro or methyl.

In some embodiments, R³is alkyl, halo, haloalkyl, deuteroalkyl, carbocyclyl, carbocyclylalkyl, heterocyclyl, heterocyclylalkyl, aryl, heteroaryl, —Si(R⁶)₃, —OR⁶, or —S(O)₂R⁷. In some embodiments, R³is alkyl, halo, haloalkyl, carbocyclyl, carbocyclylalkyl, heterocyclyl, heterocyclylalkyl, or heteroalkyl. In some embodiments, R³is optionally substituted C_1-10alkyl, halo, haloalkyl, deuteroalkyl, carbocyclyl, optionally substituted aryl, or optionally substituted heteroaryl, wherein R³is optionally substituted with halo, alkyl, alkoxy, hydroxy, —NR⁴R⁵, or —S(O)₂R⁷. In some embodiments, R³is C_1-6alkyl, C_1-6haloalkyl, C_1-6deuteroalkyl, C_3-6carbocyclyl, C_3-6carbocyclylC_1-6alkyl, C_3-6heterocyclyl, C_3-6heterocyclylC_1-6alkyl. In some embodiments, R³is alkyl, haloalkyl, carbocyclyl, carbocyclylalkyl, heterocyclyl, heterocyclylalkyl, or heteroalkyl. In some embodiments, R³is C_1-6alkyl, C_1-6fluoroalkyl, carbocyclyl, or heterocyclylalkyl. In certain embodiments, R³is C_3-8alkyl or C_1-6fluoroalkyl. In some embodiments, R³is C_1-6alkyl. In some embodiments, R³is C_1-8alkyl. In some embodiments, R³is C_2-8alkyl. In some embodiments, R³is C_3-6alkyl. In some embodiments, R³is methyl. In some embodiments, R³is ethyl. In some embodiments, R³is propyl. In some embodiments, R³is i-propyl. In some embodiments, R³is t-butyl. In some embodiments, R³is butyl. In some embodiments, R³is isobutyl. In some embodiments, R³is sec-butyl. In some embodiments, R³is carbocyclyl. In some embodiments, R³is cyclopropyl. In some embodiments, R³is cyclobutyl. In some embodiments, R³is cyclopentyl. In some embodiments, R³is cyclohexyl. In some embodiments, R³is halo. In some embodiments, R³is fluoro. In some embodiments, R³is chloro. In some embodiments, R³is bromo. In some embodiments, R³is trifluoromethyl. In some embodiments, R³is hydroxyalkyl. In some embodiments, R³is deuteromethyl. In some embodiments, R³is —Si(R⁶)₃. In some embodiments, R³is —Si(Me)₃. In some embodiments, R³is —Si(Ph)₃. In some embodiments, R³is —OR⁶. In some embodiments, R³is —S(O)₂R⁷.

In other embodiments, R³is optionally substituted aryl or optionally substituted heteroaryl. In certain specific embodiments, R³is phenyl, furanyl, pyrrolyl, oxazolyl, thiazolyl, imidazolyl, pyrazolyl, triazolyl, tetrazolyl, isoxazolyl, isothiazolyl, oxadiazolyl, thiadiazolyl, pyridinyl, pyrimidinyl, pyrazinyl, pyridazinyl, or triazinyl. In some embodiments, R³is phenyl optionally substituted with halo, alkyl, alkoxy, hydroxy, —NR⁴R⁵, or —S(O)₂R⁷. In some embodiments, R³is pyridinyl optionally substituted with halo, alkyl, alkoxy, hydroxy, —NR⁴R⁵, or —S(O)₂R⁷. In some embodiments, R³is phenyl. In some embodiments, R³is pyridinyl.

In certain embodiments, R³is optionally substituted with halo, alkyl, hydroxy, alkoxy, —NR⁴R⁵, —S(O)₂R⁷or fluoroalkyl. In some embodiments, R³is optionally substituted with chloro, fluoro, methyl, ethyl, propyl, iso-propyl, butyl, iso-butyl, sec-butyl, tert-butyl, hydroxy, methoxy, ethoxy, propoxy, iso-propoxy, dimethylamino, diethylamino, methylamino, amino, —S(O)₂Me, or trifluoromethyl. In certain embodiments, R³is optionally substituted with chloro, fluoro, methyl, hydroxy, methoxy, dimethylamino, —S(O)₂Me, or trifluoromethyl.

In some embodiments, R⁴and R⁵are each independently —H, optionally substituted alkyl, fluoroalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, —S(O)₂R⁷, or —C(O)N(R¹³)₂. In some embodiments, R⁴and R⁵are each independently —H, C_1-6alkyl, C_1-6fluoroalkyl, C_3-6carbocyclyl, C_1-6carbocyclylalkyl, C_2-6heterocyclyl, C_1-6alkylC_3-6heterocyclyl, —S(O)₂R⁷, or —C(O)N(R¹³)₂. In some embodiments, R⁴and R⁵are each independently —H, C_1-6alkyl, C_1-6fluoroalkyl, C_3-6carbocyclyl, C_3-5heterocyclyl, or —S(O)₂R⁷. In some embodiments, R⁴and R⁵are each independently —H, C_1-6alkyl, or —S(O)₂R⁷. In some embodiments, R⁴and R⁵are each independently —H or alkyl. In some embodiments, R⁴and R⁵are each independently —H, methyl, ethyl, propyl, iso-propyl, butyl, sec-butyl, iso-butyl, tert-butyl, or —S(O)₂R⁷. In some embodiments, R⁴and R⁵are each independently —H, methyl, ethyl, propyl, or iso-propyl. In some embodiments, R⁴and R⁵are —H. In some embodiments, R⁴and R⁵are methyl. In some embodiments, R⁴is methyl and R⁵is ethyl. In some embodiments, R⁴is methyl and R⁵is propyl. In some embodiments, R⁴is methyl and R⁵is iso-propyl. In some embodiments, R⁴is H and R⁵is iso-propyl. In some embodiments, R⁴and R⁵are each independently —H or —C(O)N(R¹³)₂.

In other embodiments, R⁴and R⁵attached to the same N atom are taken together with the N atom to which they are attached to form a substituted or unsubstituted 4-, 5-, or 6-membered ring heterocycle additionally containing 0-3 heteroatoms selected from —O—, —NH—, —NR⁶—, —S—, and —S(O)₂—. In some embodiments, R⁴and R⁵attached to the same N atom are taken together with the N atom to which they are attached to form a 4-, 5- or 6-membered heterocycle additionally containing 0 or 1 oxygen heteroatom. In other embodiments, R⁴and R⁵attached to the same N atom are taken together with the N atom to which they are attached to form a substituted or unsubstituted 4-, 5-, or 6-membered ring heterocycle additionally containing 1 heteroatoms selected from —O—, —NH—, —NR⁶—, —S—, and —S(O)₂—. In other embodiments, R⁴and R⁵attached to the same N atom are taken together with the N atom to which they are attached to form a 4-, 5-, or 6-membered ring heterocycle additionally containing 1 oxygen heteroatom. In other embodiments, R⁴and R⁵attached to the same N atom are taken together with the N atom to which they are attached to form a 4-, 5-, or 6-membered ring. In some embodiments, the 4-, 5-, or 6-membered ring is

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In certain embodiments, R⁴and R⁵are each independently optionally substituted with halo, alkyl, hydroxy, alkoxy, or fluoroalkyl. In some embodiments, R⁴and R⁵are each independently optionally substituted with fluoro or C_1-6alkyl. In some embodiments, R⁴and R⁵are each independently optionally substituted with fluoro or methyl.

In some embodiments, R⁶is optionally substituted alkyl, fluoroalkyl, optionally substituted aryl, optionally substituted carbocyclyl, or optionally substituted heterocyclyl. In some embodiments, R⁶is optionally substituted alkyl, fluoroalkyl, optionally substituted carbocyclyl, or optionally substituted heterocyclyl. In some embodiments, R⁶is alkyl, aryl, carbocyclyl, or heterocyclyl. In some embodiments, R⁶is alkyl, carbocyclyl, or heterocyclyl. In some embodiments, R⁶is alkyl, carbocyclyl, or fluoroalkyl. In some embodiments, R⁶is C_1-6alkyl, C_1-6fluoroalkyl, C_3-6carbocyclyl, or C_3-6heterocyclyl. In certain embodiments, R⁶is C_1-6alkyl. In certain embodiments, R⁶is methyl, ethyl, propyl, iso-propyl, butyl, sec-butyl, iso-butyl, or tert-butyl. In some embodiments, R⁶is optionally substituted phenyl.

In some embodiments, R⁷is alkyl, carbocyclyl, optionally substituted aryl, optionally substituted aralkyl, or optionally substituted heterocyclyl. In some embodiments, R⁷is C_1-6alkyl, C_3-6carbocyclyl, or aryl optionally substituted with halo or alkyl. In some embodiments, le is C_1-6alkyl, C_3-6carbocyclyl, or phenyl optionally substituted with halo or alkyl. In some embodiments, R⁷is optionally substituted benzyl. In some embodiments, R⁷is C_3-6carbocyclyl. In some embodiments, R⁷is phenyl. In some embodiments, R⁷is phenyl substituted with 1-4 C_1-6alkyl or fluoro substituents. In some embodiments, R⁷is phenyl substituted with a C_1-6alkyl or fluoro substituent. In some embodiments, R⁷is alkyl, carbocyclyl, aralkyl, or heterocyclyl.

In certain embodiments, R⁷is optionally substituted with halo, alkyl, hydroxy, alkoxy, or fluoroalkyl. In some embodiments, R⁷is optionally substituted with fluoro or C_1-6alkyl. In some embodiments, R⁷is optionally substituted with fluoro or methyl.

In some embodiments, R⁸and R⁹are each independently —H, C_1-6alkyl, C_1-6haloalkyl, halo, C_3-6carbocyclyl, C_1-6alkylC_3-6carbocyclyl, C_1-6heteroalkyl, C_3-6heterocyclyl, C_1-6alkylC_3-6heterocyclyl, —OH, —OR⁶, —NR⁴R⁵, —C(O)NR⁴R⁵, —CN, —S(O)₂R⁷, —C(O)₂H, —C(O)R⁶, or —C(O)OR⁶or R⁸and R⁹are taken together with the atom to which they are attached to form a ring containing 0-2 heteroatoms selected from the group consisting of —O—, —NH—, —NR⁶—, —S—, and —S(O)₂—. In some embodiments, R⁸and R⁹are each independently —H, C_1-6alkyl, halo, C_3-6carbocyclyl, methoxy, ethoxy, propoxy, iso-propoxy, —NH₂, —NMe₂, —NHMe, —NEt₂, —C(O)NH₂, —C(O)NMe₂, —C(O)NHMe, or —CN. In some embodiments, R⁸and R⁹are each independently —H, C_1-6alkyl, C_1-6fluoroalkyl, halo, or C_3-6carbocyclyl. In some embodiments, R⁸and R⁹are each independently —H, C_1-6alkyl, or C_3-6carbocyclyl. In some embodiments, R⁸and R⁹are each independently —H or C_1-6alkyl. In some embodiments, R⁸is —H and R⁹is C_1-6alkyl, C_1-6fluoroalkyl, or C_3-6carbocyclyl. In some embodiments, R⁸is —H and R⁹is alkyl. In some embodiments, R⁸is C_1-6alkyl, C_1-6fluoroalkyl, or C_3-6carbocyclyl and R⁹is —H. In some embodiments, R⁸is methyl and R⁹is —H. In some embodiments, R⁸and R⁹are —H. In some embodiments, R⁸and R⁹are C_1-6alkyl. In some embodiments, R⁸and R⁹are methyl.

In other embodiments, R⁸and R⁹are taken together with the atom to which they are attached to form a 3-, 4-, 5-, or 6-membered ring containing 0-2 heteroatoms selected from the group consisting of —O—, —NH—, —NR⁶—, —S—, and —S(O)₂—. In some embodiments, R⁸and R⁹are taken together with the atom to which they are attached to form a 3-, 4-, 5-, or 6-membered carbocyclic ring.

In some embodiments, R¹⁰and R¹¹are each independently —H, alkyl, halo, haloalkyl, carbocyclyl, heteroalkyl, or —OH. In other embodiments, R¹⁰and R¹¹are each independently —H, C_1-6alkyl, halo, C_1-6alkoxy, or —OH. In certain embodiments, R¹⁰and R¹¹are each independently —H or C_1-6alkyl. In certain embodiments, R¹⁰and R¹¹are each independently —H, fluoro, chloro, methyl, ethyl, propyl, iso-propyl, butyl, iso-butyl, sec-butyl, or tert-butyl. In certain embodiments, R¹⁰and R¹¹are each independently —H, fluoro, or methyl. In certain embodiments, R¹⁰and R¹¹are each independently —H or methyl. In certain embodiments, R¹⁰and R¹¹are each independently —H or fluoro. In certain embodiments, R¹⁰and R¹¹are both —H. In certain embodiments, R¹⁰and R¹¹are each independently —H or methoxy. In certain embodiments, R¹⁰and R¹¹are each independently —H or —OH. In certain embodiments, R¹⁰and R¹¹are each independently —H or fluoro. In certain embodiments, R¹⁰and R¹¹are each independently —H or chloro.

In some embodiments, R¹⁰and R¹¹are each independently —H, alkyl, halo, haloalkyl, carbocyclyl, heteroalkyl, or —OH, wherein at least one of R¹⁰or R¹¹is not H. In other embodiments, R¹⁰and R¹¹are each independently —H, C_1-6alkyl, halo, C_1-6alkoxy, or —OH, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently —H or C_1-6alkyl, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently —H, fluoro, chloro, methyl, ethyl, propyl, iso-propyl, butyl, iso-butyl, sec-butyl, or tert-butyl, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently —H, fluoro, or methyl, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently H or methyl, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently —H or fluoro, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently H or methoxy, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently —H or —OH, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently —H or fluoro, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently —H or chloro, wherein at least one of R¹⁰or R¹¹is not H.

In some embodiments, R¹⁰and R¹¹are methyl. In some embodiments, R¹⁰and R¹¹are ethyl. In some embodiments, R¹⁰and R¹¹are propyl. In some embodiments, R¹⁰and R¹¹are fluoro. In some embodiments, R¹⁰and R¹¹are chloro. In some embodiments, R¹⁰and R¹¹are bromo. In some embodiments, R¹⁰and R¹¹are methoxy. In some embodiments, R¹⁰and R¹¹are ethoxy. In some embodiments, R¹⁰and R¹¹are —OH.

In some embodiments, R¹⁰and R¹¹are taken together with the atom to which they are attached to form a 3-, 4-, 5-, or 6-membered ring. In some embodiments, R¹⁰and R¹¹are taken together with the atom to which they are attached to form a cyclopropyl, cyclobutyl, cyclopentyl, or cyclohexyl. In some embodiments, R¹⁰and R¹¹are taken together with the atom to which they are attached to form a cyclopropyl.

In certain embodiments, R¹⁰and R¹¹are optionally substituted with halo, alkyl, hydroxy, alkoxy, or fluoroalkyl. In some embodiments, R¹⁰and R¹¹are optionally substituted with fluoro or C_1-6alkyl. In some embodiments, R¹⁰and R¹¹are optionally substituted with fluoro or methyl.

In some embodiments, R¹²is hydrogen, C_1-6alkyl, C_1-6haloalkyl, C_1-6deuteroalkyl, C_3-6carbocyclyl, C_3-6carbocyclylC_1-6alkyl, C_3-6heterocyclyl, C_3-6heterocyclylC_1-6alkyl. In some embodiments, R¹²is hydrogen, alkyl, haloalkyl, hydroxy, halo, carbocyclyl, or heteroalkyl. In some embodiments, R¹²is hydrogen, C_1-6alkyl, C₁fluoroalkyl, carbocyclyl, or heterocyclylalkyl. In certain embodiments, R¹²is C_1-6alkyl or C_1-6fluoroalkyl. In some embodiments, R¹²is C_1-6alkyl, C_1-6haloalkyl, C_1-6deuteroalkyl, C_3-6carbocyclyl, C_3-6carbocyclylC_1-6alkyl, C_3-6heterocyclyl, C_3-6heterocyclylC_1-6alkyl. In some embodiments, R¹²is alkyl, haloalkyl, hydroxy, halo, carbocyclyl, or heteroalkyl. In some embodiments, R¹²is C_1-6alkyl, C_1-6fluoroalkyl, carbocyclyl, or heterocyclylalkyl. In certain embodiments, R¹²is C_1-6alkyl or C_1-6fluoroalkyl. In certain embodiments, R¹²is hydrogen. In some embodiments, R¹²is C_1-6alkyl. In some embodiments, R¹²is C_1-3alkyl. In some embodiments, R¹²is C_2-8alkyl. In some embodiments, R¹²is C_3-6alkyl. In some embodiments, R¹²is methyl. In some embodiments, R¹²is ethyl. In some embodiments, R¹²is propyl. In some embodiments, R¹²is i-propyl. In some embodiments, R¹²is t-butyl. In some embodiments, R¹²is butyl. In some embodiments, R¹²is isobutyl. In some embodiments, R¹²is sec-butyl. In some embodiments, R¹²is carbocyclyl. In some embodiments, R¹²is cyclopropyl. In some embodiments, R¹²is cyclobutyl. In some embodiments, R¹²is cyclopentyl. In some embodiments, R¹²is cyclohexyl. In some embodiments, R¹²is trifluoromethyl. In some embodiments, R¹²is hydroxyalkyl. In some embodiments, R¹²is deuteromethyl.

In certain embodiments, R¹²is optionally substituted with halo, alkyl, hydroxy, alkoxy, or fluoroalkyl. In some embodiments, R¹²is optionally substituted with fluoro or C_1-6alkyl. In some embodiments, R¹²is optionally substituted with fluoro or methyl.

In some embodiments, each R¹³is independently hydrogen, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted aralkyl, optionally substituted heterocyclyl, or optionally substituted heteroaryl. In some embodiments, each R¹³is independently hydrogen, optionally substituted alkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, or optionally substituted aryl. In some embodiments, each R¹³is independently optionally substituted alkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted aryl, or heteroaryl. In some embodiments, each le is independently alkyl or aryl. In some embodiments, each R¹³is independently hydrogen or C_1-6alkyl. In some embodiments, each le is independently hydrogen, methyl, ethyl, propyl, iso-propyl, butyl, iso-butyl, sec-butyl, tert-butyl, pentyl or hexyl. In some embodiments, each R¹³is independently hydrogen, methyl, ethyl, propyl, iso-propyl, butyl, or tert-butyl. In some embodiments, each le is independently hydrogen or methyl. In some embodiments, R¹³is H. In other embodiments, R¹³is alkyl. In other embodiments, R¹³is aryl.

In some embodiments, n is 0, 1, or 2. In some embodiments, n is 0 or 1. In some embodiments, n is 1 or 2. In some embodiments, n is 3 or 4. In some embodiments, n is 0. In some embodiments, n is 1. In some embodiments, n is 2. In some embodiments, n is 3.

Any combination of the groups described above for the various variables is contemplated herein. Throughout the specification, groups and substituents thereof are chosen by one skilled in the field to provide stable moieties and compounds.

In some embodiments, compounds described herein have the following structure of Formula (Ia):

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In some embodiments, compounds described herein have the following structure of Formula (Ib):

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In some embodiments, compounds described herein have the following structure of Formula (Ic):

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In some embodiments, compounds described herein have the following structure of Formula (Id):

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In some embodiments, compounds described herein have the following structure of Formula (Ie):

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In some embodiments, compounds described herein have the following structure of Formula (If):

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In some embodiments, compounds described herein have the following structure of Formula (Ig):

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In some embodiments, compounds described herein have the following structure of Formula (Ih):

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In some embodiments, compounds described herein have the following structure of Formula (Ii):

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In some embodiments, compounds described herein have the following structure of Formula (Ij):

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In some embodiments, compounds described herein have the following structure of Formula (Ik):

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In some embodiments, compounds described herein have the following structure of Formula (Im):

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In some embodiments, compounds described herein have the following structure of Formula (In):

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In some embodiments, compounds described herein have the following structure of Formula (Io):

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In some embodiments, compounds described herein have the following structure of Formula (Ip):

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In some embodiments, compounds described herein have the following structure of Formula (Iq):

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In some embodiments, compounds described herein have the following structure of Formula (Ir) or Formula (Is):

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- wherein
- ring B is an optionally substituted fused carbocyclyl, optionally substituted fused heterocyclyl, optionally substituted fused aryl, or optionally substituted fused heteroaryl;
- and n is 0, 1, 2, or 3.

Some embodiments provided herein describe a compound having the structure of Formula (II), or a pharmaceutically acceptable salt, solvate, or prodrug thereof:

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wherein

ring A is a heteroaryl, aryl, cycloalkyl, or heterocyclyl;
R¹is —H, —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, halo, —OR⁶, —OH, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted hydroxyalkyl, optionally substituted aryl, optionally substituted heteroaryl, —C(O)OR⁶, —C(O)NR⁴R⁵, —OC(O)OR⁶, —OC(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —S(O)R⁷, —SR⁷, —NR⁴S(O)₂NR⁴R⁵, —P(O)(OR)₂, —P(O)(R⁶)₂, —CN, —CO₂H, or —NO₂;
each R²is independently —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, halo, —OR⁶, —OH, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted hydroxyalkyl, —C(O)R⁶, —C(O)OR⁶, —C(O)NR⁴R⁵, —OC(O)OR⁶, —OC(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —S(O)R⁷, —SR⁷, —NR⁴S(O)₂NR⁴R⁵, —CN, —CO₂H, or —NO₂;
R³is optionally substituted alkyl, halo, haloalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted heteroaryl, —Si(R⁶)₃, —OR⁶, or —S(O)₂R⁷;
R⁴and R⁵are each independently —H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —S(O)₂R⁷, —C(O)N(R¹³)₂, —C(O)R⁶, or —C(O)OR⁶;
- or R⁴and R⁵attached to the same N atom are taken together with the N atom to which they are attached to form a substituted or unsubstituted heterocycle;
each R⁶is independently optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, or optionally substituted heteroaryl;
R⁷is optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted aralkyl, optionally substituted heterocyclyl, or optionally substituted heteroaryl;
each R¹³is independently H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted aralkyl, optionally substituted heterocyclyl, or optionally substituted heteroaryl;
R¹⁴is hydrogen or methyl;
and n is 0, 1, 2, 3, or 4.

In some embodiments,

ring A is aryl or bicyclic heteroaryl;
R¹is —NR⁴R⁵, halo, —OR⁶, C_1-6alkyl, C_1-6fluoroalkyl, C_3-6carbocyclyl, C_3-6heterocyclyl, aryl, heteroaryl, —C(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —NR⁴S(O)₂NR⁴R⁵, —P(O)(R⁶)₂, —P(O)(OR⁶)₂, or —CN;
each R²is independently —NR⁴R⁵, halo, —OR⁶, C_1-6alkyl, C_1-6fluoroalkyl, C_3-6carbocyclyl, C_1-6heteroalkyl, C_3-6heterocyclyl, or —CN;
R³is C_1-6alkyl, C_1-6fluoroalkyl, carbocyclyl, or heterocyclylalkyl;
R⁴and R⁵are each independently —H, C_1-6alkyl, C_1-6fluoroalkyl, C_3-6carbocyclyl, C_1-6carbocyclylalkyl, C_2-6heterocyclyl, C_1-6alkylC_3-6heterocyclyl, —S(O)₂R⁷, or —C(O)N(R¹³);
- or R⁴and R⁵attached to the same N atom are taken together with the N atom to which they are attached to form a substituted or unsubstituted 4-, 5-, or 6-membered heterocycle;
R⁶is alkyl;
R⁷is C_1-6alkyl, C_3-6carbocyclyl, or phenyl optionally substituted with halo or alkyl;
each R¹³is independently hydrogen or C_1-6alkyl;
R¹⁴is hydrogen or methyl;
and n is 0, 1, 2, 3, or 4.

In some embodiments,

ring A is phenyl, 3-benzodioxyxlyl, or 1,4-benzodioxanyl;
R¹is —NR⁴R⁵, halo, —OR⁶, alkyl, or fluoroalkyl;
each R²is independently —NR⁴R⁵, halo, alkyl, carbocyclyl, alkoxy, or —CN;
R³is C_1-6alkyl, C_1-6fluoroalkyl, carbocyclyl, deuteroalkyl, or heteroalkyl;
R⁴and R⁵are each independently —H, C_1-6alkyl, heteroalkyl, or —S(O)₂R⁷;
- or R⁴and R⁵attached to the same N atom are taken together with the N atom to which they are attached to form a substituted or unsubstituted 4-, 5-, or 6-membered heterocycle;
R⁶is alkyl;
R⁷is C_1-6alkyl, C_3-6carbocyclyl, or phenyl optionally substituted with halo or alkyl;
each R¹³is independently hydrogen or C_1-6alkyl;
R¹⁴is hydrogen;
and n is 0, 1, or 2.

In some embodiments,

ring A is phenyl;
R¹is —NR⁴R⁵, —OR⁶, alkyl, or fluoroalkyl;
each R²is independently —NR⁴R⁵, halo, alkyl;
R³is C_1-6alkyl, C_1-6fluoroalkyl, carbocyclyl, deuteroalkyl, or heteroalkyl;
R⁴and R⁵are each independently H, C_1-6alkyl, or heteroalkyl;
- or R⁴and R⁵attached to the same N atom are taken together with the N atom to which they are attached to form a substituted or unsubstituted 4-, 5-, or 6-membered heterocycle;
R⁶is alkyl;
R⁷is C_1-6alkyl, C_3-6carbocyclyl, or phenyl optionally substituted with halo or alkyl;
each R¹³is independently hydrogen or C_1-6alkyl;
R¹⁴is hydrogen;
and n is 0, 1, or 2.

In some embodiments,

ring A is 3-benzodioxyxlyl, or 1,4-benzodioxanyl;
R¹is H;
R³is C_1-6alkyl;
each R¹³is independently hydrogen or C_1-6alkyl;
R¹⁴is hydrogen;
and n is 0.

For any and all of the embodiments of Formula (II), substituents are selected from among a subset of the listed alternatives.

In some embodiments, ring A is phenyl, pyridinyl, pyrimidinyl, pyrazinyl, or pyridazinyl. In some embodiments, ring A is phenyl.

In some embodiments, R¹is —H, —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, halo, optionally substituted alkyl, fluoroalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted hydroxyalkyl, optionally substituted aryl, optionally substituted heteroaryl, —C(O)NR⁴R⁵, —OC(O)OR⁶, —OC(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —S(O)R⁷, —SR⁷, —NR⁴S(O)₂NR⁴R⁵, —P(O)(OR⁶)₂, —P(O)(R⁶)₂, —CN, or —NO₂. In some embodiments, R¹is —H, —NR⁴R⁵, halo, —OR⁶, optionally substituted alkyl, fluoroalkyl, optionally substituted carbocyclyl, optionally substituted heterocyclyl, optionally substituted aryl, optionally substituted heteroaryl, —C(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —NR⁴S(O)₂NR⁴R⁵, —P(O)(R⁶)₂, —P(O)(OR⁶)₂, or —CN. In some embodiments, R¹is —H, —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, halo, —OR⁶, optionally substituted alkyl, fluoroalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, —C(O)R⁶, —C(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —NR⁴S(O)₂NR⁴R⁵, —CN, or —CO₂H. In some embodiments, R¹is —NR⁴R⁵, halo, —OR⁶, alkyl, fluoroalkyl, carbocyclyl, heteroalkyl, heterocyclyl, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —NR⁴S(O)₂NR⁴R⁵, or —CN. In some embodiments, R¹is —NR⁴R⁵, halo, —OR⁶, C_1-6alkyl, C_1-6fluoroalkyl, C_3-6carbocyclyl, C_3-6heterocyclyl, aryl, heteroaryl, —C(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —NR⁴S(O)₂NR⁴R⁵, —P(O)(R⁶)₂, —P(O)(OR⁶)₂, or —CN. In some embodiments, R¹is —NR⁴R⁵, halo, —OR⁶, —S(O)₂NR⁴R⁵, —P(O)(R⁶)₂, or —P(O)(OR⁶)₂. In certain embodiments, R¹is —NR⁴R⁵, halo, —OR⁶, alkyl, or fluoroalkyl. In certain embodiments, R¹is halo, —OR⁶, or alkyl. In other embodiments, R¹is —NMe₂, —NHMe, —NH₂, methyl, ethyl, propyl, iso-propyl, butyl, iso-butyl, sec-butyl, tert-butyl, methoxy, ethoxy, propoxy, iso-propoxy, morpholino, or pyrrolidino. In certain embodiments, R¹is C_1-6alkyl, fluoroalkyl, C_3-6carbocyclyl, or C_3-6heterocyclyl. In some embodiments, R¹is —H. In some embodiments, R¹is —NR⁴R⁵. In some embodiments, R¹is —NMe₂. In some embodiments, R¹is —NHMe. In some embodiments, R¹is —NH₂. In some embodiments, R¹is —NMeEt. In some embodiments, R¹is —NEt₂. In some embodiments, R¹is halo. In some embodiments, R¹is fluoro. In some embodiments, R¹is chloro. In some embodiments, R¹is bromo. In some embodiments, R¹is C_1-6alkyl. In some embodiments, R¹is methyl. In some embodiments, R¹is ethyl. In some embodiments, R¹is propyl. In some embodiments, R¹is iso-propyl. In some embodiments, R¹is carbocyclyl. In some embodiments, R¹is cyclopropyl. In some embodiments, R¹is cyclobutyl. In some embodiments, R¹is cyclopentyl. In some embodiments, R¹is cyclohexyl. In some embodiments, R¹is —OR⁶. In some embodiments, R¹is methoxy. In some embodiments, R¹is ethoxy. In some embodiments, R¹is propoxy. In some embodiments, R¹is iso-propoxy. In some embodiments, R¹is heterocyclyl. In some embodiments, R¹is azetadino, morpholino, thiomorpholino, piperidino, piperazino, or pyrrolidino. In some embodiments, R¹is morpholino. In some embodiments, R¹is pyrrolidino. In some embodiments, R¹is azetadino.

In some embodiments, each R²is independently —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, halo, —OR⁶, —OH, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted hydroxyalkyl, —C(O)R⁶, —C(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —NR⁴S(O)₂NR⁴R⁵, or —CN. In some embodiments, each R²is independently —NR⁴R⁵, alkylNR⁴R⁵, halo, —OR⁶, —OH, alkyl, haloalkyl, carbocyclyl, carbocyclylalkyl, heteroalkyl, heterocyclyl, heterocyclylalkyl, hydroxyalkyl, —C(O)R⁶, —C(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —NR⁴S(O)₂NR⁴R⁵, or —CN. In some embodiments, —NR⁴R⁵, halo, —OR⁶, optionally substituted alkyl, fluoroalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, —C(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —NR⁴S(O)₂NR⁴R⁵, —CN, or —CO₂H. In certain embodiments, each R²is independently —NR⁴R⁵, halo, —OR⁶, —OH, optionally substituted alkyl, fluoroalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted hydroxyalkyl, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —NR⁴S(O)₂NR⁴R⁵, or —CN. In some embodiments, each R²is independently —NR⁴R⁵, halo, —OR⁶, alkyl, fluoroalkyl, carbocyclyl, heteroalkyl, heterocyclyl, —S(O)₂NR⁴R⁵, —NR⁴S(O)₂NR⁴R⁵, or —S(O)₂R⁷. In some embodiments, each R²is independently —NR⁴R⁵, C_1-6alkylNR⁴R⁵, halo, —OR⁶, —OH, C_1-6alkyl, C_1-6fluoroalkyl, C_3-6carbocyclyl, C_1-6alkylC_3-6carbocyclyl, C_1-6heteroalkyl, C_3-6heterocyclyl, C_1-6alkylC_3-6heterocyclyl, C_1-6hydroxyalkyl, or —CN. In some embodiments, each R²is independently —C(O)R⁶, —C(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, or —CN. In some embodiments, each R²is independently —NR⁴R⁵, halo, —OR⁶, C_1-6alkyl, C_1-6fluoroalkyl, C_3-6carbocyclyl, C_1-6heteroalkyl, C_3-6heterocyclyl, or —CN. In some embodiments, each R²is independently —NR⁴R⁵, halo, alkyl, carbocyclyl, alkoxy, or —CN. In some embodiments, each R²is independently —NR⁴R⁵, halo, alkyl, or alkoxy. In some embodiments, each R²is independently methyl, methoxy, ethyl, propyl, iso-propyl, cyclopropyl, fluoro, chloro, or —NMe₂. In some embodiments, each R²is independently methyl, methoxy, iso-propyl, cyclopropyl, fluoro, chloro, or —NMe₂. In some embodiments, R²is methoxy. In some embodiments, R²is methyl. In some embodiments R²is ethyl. In some embodiments, R²is iso-propyl. In some embodiments, R²is propyl. In some embodiments, R²is cyclopropyl. In some embodiments, R²is cyclobutyl. In some embodiments, R²is fluoro. In some embodiments, R²is chloro. In some embodiments, R²is —NMe₂. In some embodiments, R²is —NH₂. In some embodiments, R²is —NHMe. In some embodiments, R²is —NMeEt.

In some embodiments, R⁴and R⁵are each independently H, optionally substituted alkyl, fluoroalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, —S(O)₂R⁷, or —C(O)N(R¹³)₂. In some embodiments, R⁴and R⁵are each independently —H, C_1-6alkyl, C_1-6fluoroalkyl, C_3-6carbocyclyl, C_1-6carbocyclylalkyl, C_2-6heterocyclyl, C_1-6alkylC_3-6heterocyclyl, —S(O)₂R⁷, or —C(O)N(R¹³)₂. In some embodiments, R⁴and R⁵are each independently —H, C_1-6alkyl, C_1-6fluoroalkyl, C_3-6carbocyclyl, C_3-5heterocyclyl, or —S(O)₂R⁷. In some embodiments, R⁴and R⁵are each independently —H, C_1-6alkyl, or —S(O)₂R⁷. In some embodiments, R⁴and R⁵are each independently —H, methyl, ethyl, propyl, iso-propyl, butyl, sec-butyl, iso-butyl, tert-butyl, or —S(O)₂R′. In some embodiments, R⁴and R⁵are each independently —H, methyl, ethyl, propyl, or iso-propyl. In some embodiments, R⁴and R⁵are —H. In some embodiments, R⁴and R⁵are methyl. In some embodiments, R⁴is methyl and R⁵is ethyl. In some embodiments, R⁴is methyl and R⁵is propyl. In some embodiments, R⁴is methyl and R⁵is iso-propyl. In some embodiments, R⁴is H and R⁵is iso-propyl. In some embodiments, R⁴and R⁵are each independently —H or —C(O)N(R¹³)₂.

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In some embodiments, R⁷is alkyl, carbocyclyl, optionally substituted aryl, optionally substituted aralkyl, or optionally substituted heterocyclyl. In some embodiments, R⁷is C_1-6alkyl, C_3-6carbocyclyl, or aryl optionally substituted with halo or alkyl. In some embodiments, R⁷is C_1-6alkyl, C_3-6carbocyclyl, or phenyl optionally substituted with halo or alkyl. In some embodiments, le is optionally substituted benzyl. In some embodiments, R⁷is C_3-6carbocyclyl. In some embodiments, R⁷is phenyl. In some embodiments, R⁷is phenyl substituted with 1-4 C_1-6alkyl or fluoro substituents. In some embodiments, R⁷is phenyl substituted with a C_1-6alkyl or fluoro substituent. In some embodiments, R⁷is alkyl, carbocyclyl, aralkyl, or heterocyclyl.

In certain embodiments, R⁷is optionally substituted with halo, alkyl, hydroxy, alkoxy, or fluoroalkyl. In some embodiments, R⁷is optionally substituted with fluoro or C_1-6alkyl. In some embodiments, le is optionally substituted with fluoro or methyl.

In some embodiments, each R¹³is independently hydrogen, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted aralkyl, optionally substituted heterocyclyl, or optionally substituted heteroaryl. In some embodiments, each R¹³is independently hydrogen, optionally substituted alkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, or optionally substituted aryl. In some embodiments, each R¹³is independently optionally substituted alkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted aryl, or heteroaryl. In some embodiments, each R¹³is independently alkyl or aryl. In some embodiments, each R¹³is independently hydrogen or C_1-6alkyl. In some embodiments, each R¹³is independently hydrogen, methyl, ethyl, propyl, iso-propyl, butyl, iso-butyl, sec-butyl, tert-butyl, pentyl or hexyl. In some embodiments, each R¹³is independently hydrogen, methyl, ethyl, propyl, iso-propyl, butyl, or tert-butyl. In some embodiments, each R¹³is independently hydrogen or methyl. In some embodiments, R¹³is H. In other embodiments, R¹³is alkyl. In other embodiments, R¹³is aryl.

In some embodiments, R¹⁴is hydrogen or methyl.

In some embodiments,

ring A is a monocyclic or bicyclic heteroaryl, monocyclic or bicyclic aryl, monocyclic cycloalkyl, or monocyclic heterocyclyl;
R¹is —H, —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, halo, —OR⁶, —OH, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted hydroxyalkyl, —C(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —NR⁴S(O)₂NR⁴R⁵, —P(O)(OR⁶)₂, —CN, or —CO₂H;
each R²is independently —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, halo, —OR⁶, —OH, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted hydroxyalkyl, —C(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —NR⁴S(O)₂NR⁴R⁵, —CN, or —CO₂H;
R³is optionally substituted alkyl, halo, haloalkyl, deuteroalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted heteroaryl, —Si(R⁶)₃, —OR⁶, or —S(O)₂R⁷;
R⁴and R⁵are each independently —H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —S(O)₂R⁷, —C(O)N(R¹³)₂, —C(O)R⁶, or —C(O)OR⁶;
- or R⁴and R⁵attached to the same N atom are taken together with the N atom to which they are attached to form a substituted or unsubstituted 4-, 5-, or 6-membered heterocycle;
R⁶is optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, or optionally substituted heteroaryl;
R⁷is optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted aralkyl, optionally substituted heterocyclyl, or optionally substituted heteroaryl;
each R¹³is independently H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted aralkyl, optionally substituted heterocyclyl, or optionally substituted heteroaryl;
R¹⁴is hydrogen or methyl;
and n is 0, 1, 2, 3, or 4.

In some embodiments, ring A is a monocyclic or bicyclic aryl; R¹is —H, —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, halo, —OR⁶, —OH, optionally substituted alkyl, haloalkyl, or optionally substituted carbocyclyl; each R²is independently —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, halo, —OR⁶, —OH, or optionally substituted alkyl; R³is optionally substituted alkyl, halo, haloalkyl, deuteroalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heterocyclyl, or —S(O)₂R⁷; R⁴and R⁵are each independently —H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, or —C(O)OR⁶; R⁶is optionally substituted alkyl; le is optionally substituted alkyl; each R¹³is independently H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, or optionally substituted heteroalkyl; R¹⁴is hydrogen or methyl; and n is 0, 1, 2, 3, or 4.

In some embodiments, ring A is a monocyclic or bicyclic heteroaryl, monocyclic or bicyclic aryl; and R¹is —H, —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, halo, —OR⁶, —OH, optionally substituted alkyl, haloalkyl, optionally substituted hydroxyalkyl, —C(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —NR⁴S(O)₂NR⁴R⁵, —P(O)(OR⁶)₂, —CN, or —CO₂H. In some embodiments, ring A is a monocyclic or bicyclic heteroaryl, monocyclic or bicyclic aryl; and R¹is —H, —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, halo, —OR⁶, —OH, optionally substituted alkyl, or —CO₂H. In some embodiments, ring A is a monocyclic or bicyclic aryl; and R¹is —H, —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, halo, —OR⁶, —OH, optionally substituted alkyl, or —CO₂H. In some embodiments, ring A is a monocyclic or bicyclic aryl; and R¹is —H, —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, halo, —OH, or —CO₂H.

In some embodiments, ring A is a monocyclic or bicyclic heteroaryl, monocyclic or bicyclic aryl, monocyclic cycloalkyl, or monocyclic heterocyclyl; R¹is —H, —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, halo, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted hydroxyalkyl, —C(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —NR⁴S(O)₂NR⁴R⁵, —P(O)(OR⁶)₂, —CN, or —CO₂H; and R³is optionally substituted alkyl, halo, haloalkyl, deuteroalkyl, optionally substituted aryl, optionally substituted heteroaryl, —Si(R⁶)₃, —OR⁶, or —S(O)₂R⁷. In some embodiments, ring A is a monocyclic or bicyclic aryl; R¹is —H, —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, —C(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —NR⁴S(O)₂NR⁴R⁵, —P(O)(OR⁶)₂, —CN, or —CO₂H; R³is optionally substituted alkyl, halo, optionally substituted heteroaryl, —Si(R⁶)₃, —OR⁶, or —S(O)₂R⁷; and R⁴and R⁵are each independently —H, optionally substituted alkyl, or —C(O)OR⁶.

In some embodiments, ring A is a monocyclic aryl; R¹is —H, —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, —C(O)NR⁴R⁵, or —CO₂H; R³is optionally substituted alkyl, halo, or —S(O)₂R⁷; and R⁴and R⁵are each independently —H, optionally substituted alkyl, or —C(O)OR⁶. In some embodiments, ring A is a monocyclic aryl; R¹is —H, or —NR⁴R⁵; R³is optionally substituted alkyl, halo, or —S(O)₂R⁷. In some embodiments, ring A is phenyl; R¹is —H, or —NR⁴R⁵; R³is optionally substituted alkyl, or halo, and R⁴and R⁵are each independently H, optionally substituted alkyl. In some embodiments, ring A is phenyl; R¹is —H, or —NR⁴R⁵; R³is alkyl; and R⁴and R⁵are each independently alkyl. In some embodiments, ring A is phenyl; R¹is —NR⁴R⁵and R³is alkyl; and R⁴and R⁵are each independently alkyl. In some embodiments, ring A is phenyl; R¹is —NR⁴R⁵and R³is C_1-6alkyl; and R⁴and R⁵are each independently alkyl. In some embodiments, ring A is pyridyl; R¹is —H, or —NR⁴R⁵; R³is alkyl, or halo; and R⁴and R⁵are each independently alkyl. In some embodiments, ring A is phenyl; R¹is —NR⁴R⁵; R³is alkyl; and R⁴and R⁵are each independently alkyl. In some embodiments, ring A is pyridyl; R¹is —H, or —NR⁴R⁵; R³is alkyl; and R⁴and R⁵are each independently alkyl. In some embodiments, ring A is pyridyl; R¹is —NR⁴R⁵and R³is alkyl; and R⁴and R⁵are each independently alkyl. In some embodiments, ring A is pyridyl; R¹is —NR⁴R⁵and R³is C_1-6alkyl; and R⁴and R⁵are each independently alkyl.

In some embodiments, ring A is a bicyclic aryl; R¹is —H, —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, —C(O)NR⁴R⁵, or —CO₂H; R³is optionally substituted alkyl, halo, or —S(O)₂R⁷; and R⁴and R⁵are each independently —H, optionally substituted alkyl, or —C(O)OR⁶. In some embodiments, ring A is a bicyclic aryl; R¹is —H, or —NR⁴R⁵; R³is optionally substituted alkyl, halo, or —S(O)₂R⁷.

In some embodiments, ring A is benzo-1,4-dioxane; R¹is —H, or —NR⁴R⁵; R³is optionally substituted alkyl, or halo. In some embodiments, ring A is benzo-1,4-dioxane; R¹is —H, or —NR⁴R⁵; R³is optionally substituted alkyl. In some embodiments, ring A is benzo-1,4-dioxane; R¹is —NR⁴R⁵and R³is alkyl. In some embodiments, ring A is benzo-1,4-dioxane; R¹is —NR⁴R⁵and R³is C_1-6alkyl.

In some embodiments, R¹⁴is hydrogen; each R¹³is independently H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, or optionally substituted heteroaryl; and n is 0, 1, 2, 3, or 4. In some embodiments, R¹⁴is hydrogen; each R¹³is independently H, optionally substituted alkyl, haloalkyl, or optionally substituted heteroaryl; and n is 0, 1, or 2. In some embodiments, R¹⁴is methyl; each R¹³is independently H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, or optionally substituted heteroaryl; and n is 0, 1, 2, 3, or 4. In some embodiments, R¹⁴is methyl; each R¹³is independently H, optionally substituted alkyl, haloalkyl, or optionally substituted heteroaryl; and n is 0, 1, or 2.

In some embodiments, compounds described herein have the following structure of Formula (IIa):

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In some embodiments, compounds described herein have the following structure of Formula (IIb):

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In some embodiments, compounds described herein have the following structure of Formula (IIc):

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In some embodiments, compounds described herein have the following structure of Formula (IId):

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In some embodiments, compounds described herein have the following structure of Formula (IIe):

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In some embodiments, compounds described herein have the following structure of Formula (IIf):

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In some embodiments, compounds described herein have the following structure of Formula (IIg):

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In some embodiments, compounds described herein have the following structure of Formula (IIh):

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Some embodiments provided herein describe a compound having the structure of Formula (III), or a pharmaceutically acceptable salt, solvate, or prodrug thereof:

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wherein

ring A is a heteroaryl, aryl, cycloalkyl, or heterocyclyl;
R¹is —NR^4aR^5a;
each R²is independently —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, halo, —OR⁶, —OH, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted hydroxyalkyl, —C(O)R⁶, —C(O)OR⁶, —C(O)NR⁴R⁵, —OC(O)OR⁶, —OC(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —S(O)R⁷, —SR⁷, —NR⁴S(O)₂NR⁴R⁵, —CN, —CO₂H, or —NO₂;
or R¹and R²on adjacent atoms are taken together with the atoms to which they are attached to form an optionally substituted heterocycle;
R³is optionally substituted C_2-8alkyl, halo, haloalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted heteroaryl, —Si(R⁶)₃, —OR⁶, or —S(O)₂R⁷;
R^4ais C_2-8alkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —S(O)₂R⁷, —C(O)N(R¹³)₂, —C(O)R⁶, or —C(O)OR⁶;
R^5ais —H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —S(O)₂R⁷, —C(O)N(R¹³)₂, —C(O)R⁶, or —C(O)OR⁶;
or R^4aand R^5aare taken together with the N atom to which they are attached to form an optionally substituted heterocycle;
R⁴and R⁵are each independently —H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —S(O)₂R⁷, —C(O)N(R¹³)₂, —C(O)R⁶, or —C(O)OR⁶;
or R⁴and R⁵are taken together with the N atom to which they are attached to form an optionally substituted heterocycle;
each R⁶is independently optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, or optionally substituted heteroaryl;
R⁷is optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted aralkyl, optionally substituted heterocyclyl, or optionally substituted heteroaryl;
R⁸and R⁹are each independently —H, optionally substituted alkyl, haloalkyl, halo, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, —OH, —S(O)₂R⁷, —C(O)₂H, —C(O)R⁶, or —C(O)OR⁶;
or R⁸and R⁹are taken together with the atom to which they are attached to form an optionally substituted ring containing 0-2 heteroatoms selected from the group consisting of —O—, —NH—, —NR⁶—, —S—, and —S(O)₂—;
R¹⁰and R¹¹are each independently —H, optionally substituted alkyl, halo, haloalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, —OH, —S(O)₂R⁷, —C(O)₂H, —C(O)R⁶, or —C(O)OR⁶;
or R¹⁰and R¹¹are taken together with the atom to which they are attached to form an optionally substituted ring containing 0-2 heteroatoms selected from the group consisting of —O—, —NH—, —NR⁶—, —S—, and —S(O)₂—;
R¹²is hydrogen, optionally substituted alkyl, haloalkyl, hydroxy, halo, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, or optionally substituted heteroalkyl;
each R¹³is independently H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted aralkyl, optionally substituted heterocyclyl, or optionally substituted heteroaryl; and
n is 0, 1, 2, 3, or 4.

In some embodiments of a compound of Formula (III):

ring A is aryl;
R¹is —NR^4aR^5a;
each R²is independently —NR⁴R⁵, halo, or optionally substituted alkyl;
or R¹and R²on adjacent atoms are taken together with the atoms to which they are attached to form an optionally substituted heterocycle;
R³is optionally substituted C_2-8alkyl, haloalkyl, or optionally substituted carbocyclyl;
R^4ais C_2-8alkyl;
R^5ais —H or optionally substituted alkyl;
or R^4aand R^5aare taken together with the N atom to which they are attached to form an optionally substituted heterocycle;
R⁴and R⁵are each independently —H or optionally substituted alkyl;
or R⁴and R⁵are taken together with the N atom to which they are attached to form an optionally substituted heterocycle;
R⁸and R⁹are each independently —H or optionally substituted alkyl;
or R⁸and R⁹are taken together with the atom to which they are attached to form an optionally substituted ring containing 0-2 heteroatoms selected from the group consisting of —O—, —NH—, —S—, and —S(O)₂—;
R¹⁰and R¹¹are each independently —H or optionally substituted alkyl;
or R¹⁰and R¹¹are taken together with the atom to which they are attached to form an optionally substituted ring containing 0-2 heteroatoms selected from the group consisting of —O—, —NH—, —S—, and —S(O)₂—;
R¹²is hydrogen or optionally substituted alkyl; and
n is 0 or 1.

In some embodiments of a compound of Formula (III):

ring A is aryl;
R¹is —NR^4aR^5a;
each R²is independently halo or alkyl;
or R¹and R²on adjacent atoms are taken together with the atoms to which they are attached to form an optionally substituted heterocycle;
R³is C_2-8alkyl, haloalkyl, or carbocyclyl;
R^4ais C_3-6alkyl;
R^5ais —H or C_1-6alkyl;
or R^4aand R^5aare taken together with the N atom to which they are attached to form an optionally substituted heterocycle;
R⁸and R⁹are each independently H or methyl;
R¹⁰and R¹¹are each —H;
R¹²is hydrogen or methyl; and
n is 0 or 1.

For any and all of the embodiments of Formula (III), substituents are selected from among a subset of the listed alternatives.

In some embodiments, ring A is phenyl, pyridinyl, pyrimidinyl, pyrazinyl, or pyridazinyl. In some embodiments, ring A is phenyl or pyridinyl. In some embodiments, ring A is phenyl.

In some embodiments, R¹is —NR^4aR^5a, R^4ais C_2-8alkyl, and R^5ais —H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —S(O)₂R⁷, —C(O)N(R¹³)₂, —C(O)R⁶, or —C(O)OR⁶. In some embodiments, R¹is —NR^4aR^5a, R^4ais C_2-8alkyl, and R^5ais —H or optionally substituted alkyl. In some embodiments, R¹is —NR^4aR^5a, R^4ais C_2-8alkyl, and R^5ais —H or alkyl. In some embodiments, R¹is —NR^4aR^5a, R^4ais C_2-8alkyl, and R^5ais —H or C_1-6alkyl. In some embodiments, R¹is —NR^4aR^5a, R^4ais ethyl, i-propyl, or t-butyl, and R^5ais —H or C_1-6alkyl. In some embodiments, R¹is —NR^4aR^5a, R^4ais ethyl, i-propyl, or t-butyl, and R^5ais C_1-6alkyl.

In some embodiments, R¹is —NR^4aR^5a, R^4ais C_3-6alkyl, and R^5ais H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —S(O)₂R⁷, —C(O)N(R¹³)₂, —C(O)R⁶, or —C(O)OR⁶. In some embodiments, R¹is —NR^4aR^5a, R^4ais C_3-6alkyl, and R^5ais —H or optionally substituted alkyl. In some embodiments, R¹is —NR^4aR^5a, R^4ais C_3-6alkyl, and R^5ais H or alkyl. In some embodiments, R¹is —NR^4aR^5a, R^4ais C_3-6alkyl, and R^5ais —H or C_1-6alkyl. In some embodiments, R¹is —NR^4aR^5a, R^4ais i-propyl or t-butyl, and R^5ais —H or C_1-6alkyl. In some embodiments, R¹is —NR^4aR^5a, R^4ais i-propyl or t-butyl, and R^5ais C_1-6alkyl.

In some embodiments, R¹is —NR^4aR^5a, R^4ais C_2-4alkyl, and R^5ais —H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —S(O)₂R⁷, —C(O)N(R¹³)₂, —C(O)R⁶, or —C(O)OR⁶. In some embodiments, R¹is —NR^4aR^5a, R^4ais C_2-4alkyl, and R^5ais H or optionally substituted alkyl. In some embodiments, R¹is —NR^4aR^5a, R^4ais C_2-4alkyl, and R^5ais H or alkyl. In some embodiments, R¹is —NR^4aR^5a, R^4ais C_2-4alkyl, and R^5ais —H or C_1-6alkyl. In some embodiments, R¹is —NR^4aR^5a, R^4ais ethyl, i-propyl, or t-butyl, and R^5ais H or C_1-6alkyl. In some embodiments, R¹is —NR^4aR^5a, R^4ais ethyl, i-propyl, or t-butyl, and R^5ais C_1-6alkyl.

In some embodiments, R¹is —NMeEt. In some embodiments, R¹is —NEt₂. In some embodiments, R¹is —NMeiPr. In some embodiments, R¹is —NHEt. In some embodiments, R¹is —NHiPr.

In some embodiments, R¹is —NR^4aR^5aand R^4aand R^5aare taken together with the N atom to which they are attached to form an optionally substituted heterocycle. In other embodiments, R¹is —NR^4aR^5aand R^4aand R^5aare taken together with the N atom to which they are attached to form an optionally substituted 4-, 5-, or 6-membered ring heterocycle additionally containing 0-3 heteroatoms selected from —O—, —NH—, —NR⁶—, —S—, and —S(O)₂—. In some embodiments, R¹is —NR^4aR^5aand R^4aand R^5aare taken together with the N atom to which they are attached to form an optionally substituted 4-, 5- or 6-membered heterocycle additionally containing 0 or 1 oxygen heteroatom. In other embodiments, R¹is —NR^4aR^5aand R^4aand R^5aare taken together with the N atom to which they are attached to form an optionally substituted 4-, 5-, or 6-membered ring heterocycle additionally containing 1 heteroatom selected from —O—, —NH—, —NR⁶—, —S—, and —S(O)₂—. In other embodiments, R¹is —NR^4aR^5aand R^4aand R^5aare taken together with the N atom to which they are attached to form a 4-, 5-, or 6-membered ring. In other embodiments, R¹is —NR^4aR^5aand R^4aand R^5aare taken together with the N atom to which they are attached to form

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In other embodiments, R¹is —NR^4aR^5aand R^4aand R^5aare taken together with the N atom to which they are attached to form an optionally substituted pyrrolidinyl, an optionally substituted morpholinyl, an optionally substituted thiomorpholinyl an optionally substituted piperidinyl, or an optionally substituted piperazinyl.

In some embodiments, R¹and R²on adjacent atoms are taken together with the atoms to which they are attached to form an optionally substituted heterocycle. In some embodiments, R¹and R²on adjacent atoms are taken together with the atoms to which they are attached to form a heterocycle. In some embodiments, R¹and R²on adjacent atoms are taken together with the atoms to which they are attached to form a 1,4-dioxane or a 1,3-dioxolane.

In some embodiments, R³is optionally substituted C_2-8alkyl, halo, haloalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted heteroaryl, —Si(R⁶)₃, —OR⁶, or —S(O)₂R⁷. In some embodiments, R³is C_2-8alkyl, halo, haloalkyl, carbocyclyl, carbocyclylalkyl, heterocyclyl, heterocyclylalkyl, heteroalkyl, aryl, optionally substituted heteroaryl, —Si(R⁶)₃, —OR⁶, or —S(O)₂R⁷. In some embodiments, R³is C_2-8alkyl, halo, haloalkyl, carbocyclyl, carbocyclylalkyl, heterocyclyl, heterocyclylalkyl, or heteroalkyl. In some embodiments, R³is C_2-8alkyl, halo, haloalkyl, deuteroalkyl,or carbocyclyl, wherein R³is optionally substituted with halo, alkyl, alkoxy, hydroxy, —NR⁴R⁵, or —S(O)₂R⁷. In some embodiments, R³is C_2-8alkyl, C_1-6haloalkyl, or C_3-6carbocyclyl.

In some embodiments, R³is CF₃, t-butyl, or cyclopropyl. In some embodiments, R³is C_4-8alkyl. In some embodiments, R³is t-butyl. In some embodiments, R³is haloalkyl. In some embodiments, R³is CF₃. In some embodiments, R³is optionally substituted carbocyclyl. In some embodiments, R³is cyclopropyl.

In some embodiments, R¹⁰and R¹¹are each independently —H, alkyl, halo, haloalkyl, carbocyclyl, heteroalkyl, or —OH, wherein at least one of R¹⁰or R¹¹is not H. In other embodiments, R¹⁰and R¹¹are each independently —H, C_1-6alkyl, halo, C_1-6alkoxy, or —OH, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently —H or C_1-6alkyl, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently —H, fluoro, chloro, methyl, ethyl, propyl, iso-propyl, butyl, iso-butyl, sec-butyl, or tert-butyl, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently —H, fluoro, or methyl, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently —H or methyl, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently —H or fluoro, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently —H or methoxy, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently —H or —OH, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently —H or fluoro, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently —H or chloro, wherein at least one of R¹⁰or R¹¹is not H.

In some embodiments, R¹²is hydrogen, C_1-6alkyl, C_1-6haloalkyl, C_1-6deuteroalkyl, C_3-6carbocyclyl, C_3-6carbocyclylC_1-6alkyl, C_3-6heterocyclyl, C_3-6heterocyclylC_1-6alkyl. In some embodiments, R¹²is hydrogen, alkyl, haloalkyl, hydroxy, halo, carbocyclyl, or heteroalkyl. In some embodiments, R¹²is hydrogen, C_1-6alkyl, C_1-6fluoroalkyl, carbocyclyl, or heterocyclylalkyl. In certain embodiments, R¹²is C_1-6alkyl or C_1-6fluoroalkyl. In some embodiments, R¹²is C_1-6alkyl, C_1-6haloalkyl, C_1-6deuteroalkyl, C_3-6carbocyclyl, C_3-6carbocyclylC_1-6alkyl, C_3-6heterocyclyl, C_3-6heterocyclylC_1-6alkyl. In some embodiments, R¹²is alkyl, haloalkyl, hydroxy, halo, carbocyclyl, or heteroalkyl. In some embodiments, R¹²is C_1-6alkyl, C_1-6fluoroalkyl, carbocyclyl, or heterocyclylalkyl. In certain embodiments, R¹²is C_1-6alkyl or C_1-6fluoroalkyl. In certain embodiments, R¹²is hydrogen. In some embodiments, R¹²is C_1-6alkyl. In some embodiments, R¹²is C_1-3alkyl. In some embodiments, R¹²is C_2-8alkyl. In some embodiments, R¹²is C_3-6alkyl. In some embodiments, R¹²is methyl. In some embodiments, R¹²is ethyl. In some embodiments, R¹²is propyl. In some embodiments, R¹²is i-propyl. In some embodiments, R¹²is t-butyl. In some embodiments, R¹²is butyl. In some embodiments, R¹²is isobutyl. In some embodiments, R¹²is sec-butyl. In some embodiments, R¹²is carbocyclyl. In some embodiments, R¹²is cyclopropyl. In some embodiments, R¹²is cyclobutyl. In some embodiments, R¹²is cyclopentyl. In some embodiments, R¹²is cyclohexyl. In some embodiments, R¹²is trifluoromethyl. In some embodiments, R¹²is hydroxyalkyl. In some embodiments, R¹²is deuteromethyl.

In some embodiments, R⁴and R⁵are each independently —H, optionally substituted alkyl, fluoroalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, —S(O)₂R⁷, or —C(O)N(R¹³)₂. In some embodiments, R⁴and R⁵are each independently —H, C_1-6alkyl, C_1-6fluoroalkyl, C_3-6carbocyclyl, C_1-6carbocyclylalkyl, C_2-6heterocyclyl, C_1-6alkylC_3-6heterocyclyl, —S(O)₂R⁷, or —C(O)N(R¹³)₂. In some embodiments, R⁴and R⁵are each independently —H, C_1-6alkyl, C_1-6fluoroalkyl, C_3-6carbocyclyl, C_3-5heterocyclyl, or —S(O)₂R⁷. In some embodiments, R⁴and R⁵are each independently —H, C_1-6alkyl, or —S(O)₂R⁷. In some embodiments, R⁴and R⁵are each independently —H, methyl, ethyl, propyl, iso-propyl, butyl, sec-butyl, iso-butyl, tert-butyl, or —S(O)₂R⁷. In some embodiments, R⁴and R⁵are each independently —H, methyl, ethyl, propyl, or iso-propyl. In some embodiments, R⁴and R⁵are —H. In some embodiments, R⁴and R⁵are methyl. In some embodiments, R⁴is methyl and R⁵is ethyl. In some embodiments, R⁴is methyl and R⁵is propyl. In some embodiments, R⁴is methyl and R⁵is iso-propyl. In some embodiments, R⁴is H and R⁵is iso-propyl. In some embodiments, R⁴and R⁵are each independently —H or —C(O)N(R¹³)₂.

In other embodiments, R⁴and R⁵attached to the same N atom are taken together with the N atom to which they are attached to form an optionally substituted 4-, 5-, or 6-membered ring heterocycle additionally containing 0-3 heteroatoms selected from —O—, —NH—, —NR⁶—, —S—, and —S(O)₂. In some embodiments, R⁴and R⁵attached to the same N atom are taken together with the N atom to which they are attached to form a 4-, 5- or 6-membered heterocycle additionally containing 0 or 1 oxygen heteroatom. In other embodiments, R⁴and R⁵attached to the same N atom are taken together with the N atom to which they are attached to form an optionally substituted 4-, 5-, or 6-membered ring heterocycle additionally containing 1 heteroatoms selected from —O—, —NH—, —NR⁶—, —S—, and —S(O)₂—. In other embodiments, R⁴and R⁵attached to the same N atom are taken together with the N atom to which they are attached to form a 4-, 5-, or 6-membered ring heterocycle additionally containing 1 oxygen heteroatom. In other embodiments, R⁴and R⁵attached to the same N atom are taken together with the N atom to which they are attached to form a 4-, 5-, or 6-membered ring.

In certain embodiments, R⁷is optionally substituted with halo, alkyl, hydroxy, alkoxy, or fluoroalkyl. In some embodiments, le is optionally substituted with fluoro or C_1-6alkyl. In some embodiments, R⁷is optionally substituted with fluoro or methyl.

In some embodiments, each R¹³is independently hydrogen, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted aralkyl, optionally substituted heterocyclyl, or optionally substituted heteroaryl. In some embodiments, each R¹³is independently hydrogen, optionally substituted alkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, or optionally substituted aryl. In some embodiments, each R¹³is independently optionally substituted alkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted aryl, or heteroaryl. In some embodiments, each R¹³is independently alkyl or aryl. In some embodiments, each R¹³is independently hydrogen or C_1-6alkyl. In some embodiments, each R¹³is independently hydrogen, methyl, ethyl, propyl, iso-propyl, butyl, iso-butyl, sec-butyl, tert-butyl, pentyl or hexyl. In some embodiments, each R¹³is independently hydrogen, methyl, ethyl, propyl, iso-propyl, butyl, or tert-butyl. In some embodiments, each R¹³is independently hydrogen or methyl. In some embodiments, R¹³is H. In other embodiments, R¹³is alkyl. In other embodiments, R¹³is aryl.

In some embodiments, compounds described herein have the following structure of Formula (IIIa):

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In some embodiments, compounds described herein have the following structure of Formula (IIIb):

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In some embodiments, compounds described herein have the following structure of Formula (IIIc):

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In some embodiments, compounds described herein have the following structure of Formula (IIId):

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In some embodiments, compounds described herein have the following structure of Formula (IIIe):

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In some embodiments, compounds described herein have the following structure of Formula (IIIf):

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In some embodiments, compounds described herein have the following structure of Formula (IIIg):

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In some embodiments, compounds described herein have the following structure of Formula (IIIh):

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In some embodiments of a compound of Formula (IIIa), (IIIb), (IIIc), (IIId), (IIIe), (IIIf), (IIIg), or (IIIh):

ring A is aryl;
R¹is —NR^4aR^5a;
R²is independently —NR⁴R⁵, halo, or optionally substituted alkyl;
or R¹and R²on adjacent atoms are taken together with the atoms to which they are attached to form an optionally substituted heterocycle;
R³is optionally substituted C_2-8alkyl, haloalkyl, or optionally substituted carbocyclyl;
R^4ais C_2-8alkyl;
R^5ais —H or optionally substituted alkyl;
or R^4aand R^5aare taken together with the N atom to which they are attached to form an optionally substituted heterocycle;
R⁴and R⁵are each independently —H or optionally substituted alkyl;
or R⁴and R⁵are taken together with the N atom to which they are attached to form an optionally substituted heterocycle;
R⁸and R⁹are each independently —H or optionally substituted alkyl;
or R⁸and R⁹are taken together with the atom to which they are attached to form an optionally substituted ring containing 0-2 heteroatoms selected from the group consisting of —O—, —NH—, —S—, and —S(O)₂—;
R¹⁰and R¹¹are each independently —H or optionally substituted alkyl;
or R¹⁰and R¹¹are taken together with the atom to which they are attached to form an optionally substituted ring containing 0-2 heteroatoms selected from the group consisting of —O—, —NH—, —S—, and —S(O)₂—; and
R¹²is hydrogen or optionally substituted alkyl.

Some embodiments provided herein describe a compound having the structure of Formula (IV), or a pharmaceutically acceptable salt, solvate, or prodrug thereof:

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wherein

ring A is a heteroaryl, aryl, cycloalkyl, or heterocyclyl;
R¹is —NR^4aR^5a;
each R²is independently —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, halo, —OR⁶, —OH, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted hydroxyalkyl, —C(O)R⁶, —C(O)OR⁶, —C(O)NR⁴R⁵, —OC(O)OR⁶, —OC(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —S(O)R⁷, —SR⁷, —NR⁴S(O)₂NR⁴R⁵, —CN, —CO₂H, or —NO₂;
or R¹and R²on adjacent atoms are taken together with the atoms to which they are attached to form an optionally substituted heterocycle;
R³is optionally substituted alkyl;
R^4ais C_2-8alkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —S(O)₂R⁷, —C(O)N(R¹³)₂, —C(O)R⁶, or —C(O)OR⁶;
R^5ais —H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —S(O)₂R⁷, —C(O)N(R¹³)₂, —C(O)R⁶, or —C(O)OR⁶;
R⁴and R⁵are each independently H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —S(O)₂R⁷, —C(O)N(R¹³)₂, —C(O)R⁶, or —C(O)OR⁶;
or R⁴and R⁵are taken together with the N atom to which they are attached to form an optionally substituted heterocycle;
each R⁶is independently optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, or optionally substituted heteroaryl;
R⁷is optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted aralkyl, optionally substituted heterocyclyl, or optionally substituted heteroaryl;
R⁸and R⁹are each independently —H, optionally substituted alkyl, haloalkyl, halo, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, —OH, —S(O)₂R⁷, —C(O)₂H, —C(O)R⁶, or —C(O)OR⁶;
or R⁸and R⁹are taken together with the atom to which they are attached to form an optionally substituted ring containing 0-2 heteroatoms selected from the group consisting of —O—, —NH—, —NR⁶—, —S—, and —S(O)₂—;
R¹⁰and R¹¹are each independently H, optionally substituted alkyl, halo, haloalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, —OH, —S(O)₂R′, —C(O)₂H, —C(O)R⁶, or —C(O)OR⁶;
or R¹⁰and R¹¹are taken together with the atom to which they are attached to form an optionally substituted ring containing 0-2 heteroatoms selected from the group consisting of —O—, —NH—, —NR⁶—, —S—, and —S(O)₂—;
R¹²is hydrogen, optionally substituted alkyl, haloalkyl, hydroxy, halo, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, or optionally substituted heteroalkyl;
each R¹³is independently H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted aralkyl, optionally substituted heterocyclyl, or optionally substituted heteroaryl; and
n is 0, 1, 2, 3, or 4.

In some embodiments of a compound of Formula (IV):

ring A is aryl;
R¹is —NR^4aR^5a;
R²is independently —NR⁴R⁵, halo, or optionally substituted alkyl;
or R¹and R²on adjacent atoms are taken together with the atoms to which they are attached to form an optionally substituted heterocycle;
R³is optionally substituted alkyl;
R^4ais C_2-8alkyl;
R^5ais —H or optionally substituted alkyl;
R⁴and R⁵are each independently —H or optionally substituted alkyl;
or R⁴and R⁵are taken together with the N atom to which they are attached to form an optionally substituted heterocycle;
R⁸and R⁹are each independently —H or optionally substituted alkyl;
or R⁸and R⁹are taken together with the atom to which they are attached to form an optionally substituted ring containing 0-2 heteroatoms selected from the group consisting of —O—, —NH—, —S—, and —S(O)₂—;
R¹⁰and R¹¹are each independently —H or optionally substituted alkyl;
or R¹⁰and R¹¹are taken together with the atom to which they are attached to form an optionally substituted ring containing 0-2 heteroatoms selected from the group consisting of —O—, —NH—, —S—, and —S(O)₂—; and
R¹²is hydrogen or optionally substituted alkyl.

Some embodiments provided herein describe a compound having the structure of Formula (V), or a pharmaceutically acceptable salt, solvate, or prodrug thereof:

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wherein

ring A is a heteroaryl, aryl, cycloalkyl, or heterocyclyl;
R¹is —NR^4aR^5a, optionally substituted alkylNR⁴R⁵, halo, —OR⁶, —OH, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted hydroxyalkyl, —C(O)R⁶, —C(O)OR⁶, —C(O)NR⁴R⁵, —OC(O)OR⁶, —OC(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —S(O)R⁷, —SR⁷, —NR⁴S(O)₂NR⁴R⁵, —CN, —CO₂H, or —NO₂;
each R²is independently —NR⁴R⁵, optionally substituted alkylNR⁴R⁵, halo, —OR⁶, —OH, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, optionally substituted hydroxyalkyl, —C(O)R⁶, —C(O)OR⁶, —C(O)NR⁴R⁵, —OC(O)OR⁶, —OC(O)NR⁴R⁵, —S(O)₂NR⁴R⁵, —S(O)₂R⁷, —S(O)R⁷, —SR⁷, —NR⁴S(O)₂NR⁴R⁵, —CN, —CO₂H, or —NO₂;
or R¹and R²on adjacent atoms are taken together with the atoms to which they are attached to form an optionally substituted heterocycle;
R³is optionally substituted carbocyclyl;
R^4ais optionally substituted alkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —S(O)₂R⁷, 13 C(O)N(R¹³)₂, —C(O)R⁶, or —C(O)OR⁶;
R^5ais —H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —S(O)₂R⁷, —C(O)N(R¹³)₂, —C(O)R⁶, or —C(O)OR⁶;
or R^4aand R^5aare taken together with the N atom to which they are attached to form an optionally substituted heterocycle;
R⁴and R⁵are each independently —H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —S(O)₂R⁷, —C(O)N(R¹³)₂, —C(O)R⁶, or —C(O)OR⁶;
or R⁴and R⁵are taken together with the N atom to which they are attached to form an optionally substituted heterocycle;
each R⁶is independently optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, or optionally substituted heteroaryl;
R⁷is optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted aralkyl, optionally substituted heterocyclyl, or optionally substituted heteroaryl;
R⁸and R⁹are each independently H, optionally substituted alkyl, haloalkyl, halo, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, —OH, —S(O)₂R⁷, —C(O)₂H, —C(O)R⁶, or —C(O)OR⁶;
or R⁸and R⁹are taken together with the atom to which they are attached to form an optionally substituted ring containing 0-2 heteroatoms selected from the group consisting of —O—, —NH—, —NR⁶—, —S—, and —S(O)₂—;
R¹⁰and R¹¹are each independently —H, optionally substituted alkyl, halo, haloalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heteroalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, —OH, —S(O)₂R⁷, —C(O)₂H, —C(O)R⁶, or —C(O)OR⁶;
or R¹⁰and R¹¹are taken together with the atom to which they are attached to form an optionally substituted ring containing 0-2 heteroatoms selected from the group consisting of —O—, —NH—, —NR⁶—, —S—, and —S(O)₂—;
R¹²is hydrogen;
each R¹³is independently H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted aralkyl, optionally substituted heterocyclyl, or optionally substituted heteroaryl; and
n is 0, 1, 2, 3, or 4.

In some embodiments of a compound of Formula (V):

ring A is aryl;
R¹is —NR^4aR^5a;
each R²is independently —NR⁴R⁵, halo, or optionally substituted alkyl;
or R¹and R²on adjacent atoms are taken together with the atoms to which they are attached to form an optionally substituted heterocycle;
R³is optionally substituted carbocyclyl;
R^4ais optionally substituted alkyl;
R^5ais —H or optionally substituted alkyl;
or R^4aand R^5aare taken together with the N atom to which they are attached to form an optionally substituted heterocycle;
R⁴and R⁵are each independently —H or optionally substituted alkyl;
or R⁴and R⁵are taken together with the N atom to which they are attached to form an optionally substituted heterocycle;
R⁸and R⁹are each independently H or optionally substituted alkyl;
or R⁸and R⁹are taken together with the atom to which they are attached to form an optionally substituted ring containing 0-2 heteroatoms selected from the group consisting of —O—, —NH—, —S—, and —S(O)₂—;
R¹⁰and R¹¹are each independently —H or optionally substituted alkyl;
or R¹⁰and R¹¹are taken together with the atom to which they are attached to form an optionally substituted ring containing 0-2 heteroatoms selected from the group consisting of —O—, —NH—, —S—, and —S(O)₂—;
R¹²is hydrogen; and
n is 0 or 1.

For any and all of the embodiments of Formulas (IV) and (V), substituents are selected from among a subset of the listed alternatives.

In some embodiments, ring A is phenyl, pyridinyl, pyrimidinyl, pyrazinyl, or pyridazinyl. In some embodiments, ring A is phenyl or pyridinyl. In some embodiments, ring A is phenyl.

In some embodiments, R¹is Nlealea, R^4ais C_2-8alkyl, and R^5ais —H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —S(O)₂R⁷, —C(O)N(R¹³)₂, —C(O)R⁶, or —C(O)OR⁶. In some embodiments, R¹is —NR^4aR^5a, R^4ais C_2-8alkyl, and R^5ais —H or optionally substituted alkyl. In some embodiments, R¹is —NR^4aR^5a, R^4ais C_2-8alkyl, and R^5ais —H or alkyl. In some embodiments, R¹is —NR^4aR^5a, R^4ais C_2-8alkyl, and R^5ais —H or C_1-6alkyl. In some embodiments, R¹is —NR^4aR^5a, R^4ais ethyl, i-propyl, or t-butyl, and R^5ais —H or C_1-6alkyl. In some embodiments, R¹is —NR^4aR^5a, R^4ais ethyl, i-propyl, or t-butyl, and R^5ais C_1-6alkyl.

In some embodiments, R¹is —NR^4aR^5a, R^4ais C_3-6alkyl, and R^5ais —H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —S(O)₂R⁷, —C(O)N(R¹³)₂, —C(O)R⁶, or —C(O)OR⁶. In some embodiments, R¹is —NR^4aR^5a, R^4ais C_3-6alkyl, and R^5ais —H or optionally substituted alkyl. In some embodiments, R¹is —NR^4aR^5a, R^4ais C_3-6alkyl, and R^5ais —H or alkyl. In some embodiments, R¹is —NR^4aR^5a, R^4ais C_3-6alkyl, and R^5ais —H or C_1-6alkyl. In some embodiments, R¹is —NR^4aR^5a, R^4ais i-propyl or t-butyl, and R^5ais —H or C_1-6alkyl. In some embodiments, R¹is —NR^4aR^5a, R^4ais i-propyl or t-butyl, and R^5ais C_1-6alkyl.

In some embodiments, R¹is —NR^4aR^5a, R^4ais C_2-4alkyl, and R^5ais —H, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —S(O)₂R⁷, —C(O)N(R¹³)₂, —C(O)R⁶, or —C(O)OR⁶. In some embodiments, R¹is —NR^4aR^5a, R^4ais C_2-4alkyl, and R^5ais —H or optionally substituted alkyl. In some embodiments, R¹is —NR^4aR^5a, R^4ais C_2-4alkyl, and R^5ais —H or alkyl. In some embodiments, R¹is —NR^4aR^5a, R^4ais C_2-4alkyl, and R^5ais —H or C_1-6alkyl. In some embodiments, R¹is —NR^4aR^5a, R^4ais ethyl, i-propyl, or t-butyl, and R^5ais —H or C_1-6alkyl. In some embodiments, R¹is —NR^4aR^5a, R^4ais ethyl, i-propyl, or t-butyl, and R^5ais C_1-6alkyl.

In some embodiments, R¹is —NMeEt. In some embodiments, R¹is —NEt₂. In some embodiments, R¹is —NMeiPr. In some embodiments, R¹is —NHEt. In some embodiments, R¹is —NHiPr.

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In other embodiments, R¹is —NR^4aR^5aand R^4aand R^5aare taken together with the N atom to which they are attached to form an optionally substituted pyrrolidinyl, an optionally substituted morpholinyl, an optionally substituted thiomorpholinyl, an optionally substituted piperidinyl, or an optionally substituted piperazinyl.

In some embodiments, R¹⁰and R¹¹are each independently —H, alkyl, halo, haloalkyl, carbocyclyl, heteroalkyl, or —OH, wherein at least one of R¹⁰or R¹¹is not H. In other embodiments, R¹⁰and R¹¹are each independently —H, C_1-6alkyl, halo, C_1-6alkoxy, or —OH, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently —H or C_1-6alkyl, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently —H, fluoro, chloro, methyl, ethyl, propyl, iso-propyl, butyl, iso-butyl, sec-butyl, or tert-butyl, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently —H, fluoro, or methyl, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently —H or methyl, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently —H or fluoro, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently —H or methoxy, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently —H or —OH, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently —H or fluoro, wherein at least one of R¹⁰or R¹¹is not H. In certain embodiments, R¹⁰and R¹¹are each independently H or chloro, wherein at least one of R¹⁰or R¹¹is not H.

In some embodiments, R¹²is hydrogen, C_1-6alkyl, C_1-6haloalkyl, C_1-6deuteroalkyl, C_3-6carbocyclyl, C_3-6carbocyclylC_1-6alkyl, C_3-6heterocyclyl, C_3-6heterocyclylC_1-6alkyl. In some embodiments, R¹²is hydrogen, alkyl, haloalkyl, hydroxy, halo, carbocyclyl, or heteroalkyl. In some embodiments, R¹²is hydrogen, C_1-6alkyl, C_1-6fluoroalkyl, carbocyclyl, or heterocyclylalkyl. In certain embodiments, R¹²is C_1-6alkyl or C_1-6fluoroalkyl. In some embodiments, R¹²is C_1-6alkyl, C_1-6haloalkyl, C_1-6deuteroalkyl, C_3-6carbocyclyl, C_3-6carbocyclylC_1-6alkyl, C_3-6heterocyclyl, C_3-6heterocyclylC_1-6alkyl. In some embodiments, R¹²is alkyl, haloalkyl, hydroxy, halo, carbocyclyl, or heteroalkyl. In some embodiments, R¹²is C_1-6alkyl, C_1-6fluoroalkyl, carbocyclyl, or heterocyclylalkyl. In certain embodiments, R¹²is C_1-6alkyl or C_1-6fluoroalkyl. In certain embodiments, R¹²is hydrogen. In some embodiments, R¹²is C_1-6alkyl. In some embodiments, R¹²is C_1-3alkyl. In some embodiments, R¹²is C_2-8alkyl. In some embodiments, R¹²is C_3-6alkyl. In some embodiments, R¹²is methyl. In some embodiments, R¹²is ethyl. In some embodiments, R¹²is propyl. In some embodiments, R¹²is i-propyl. In some embodiments, R¹²is t-butyl. In some embodiments, R¹²is butyl. In some embodiments, R¹²is isobutyl. In some embodiments, R¹²is sec-butyl. In some embodiments, R¹²is carbocyclyl. In some embodiments, R¹²is cyclopropyl. In some embodiments, R¹²is cyclobutyl. In some embodiments, R¹²is cyclopentyl. In some embodiments, R¹²is cyclohexyl. In some embodiments, R¹²is trifluoromethyl. In some embodiments, R¹²is hydroxyalkyl. In some embodiments, R¹²is deuteromethyl.

In some embodiments, R⁴and R⁵are each independently —H, optionally substituted alkyl, fluoroalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted heterocyclyl, optionally substituted heterocyclylalkyl, —S(O)₂R⁷, or —C(O)N(R¹³)₂. In some embodiments, R⁴and R⁵are each independently —H, C_1-6alkyl, C_1-6fluoroalkyl, C_3-6carbocyclyl, C_1-6carbocyclylalkyl, C_2-6heterocyclyl, C_1-6alkylC_3-6heterocyclyl, —S(O)₂R⁷, or —C(O)N(R¹³)₂. In some embodiments, R⁴and R⁵are each independently —H, C_1-6alkyl, C_1-6fluoroalkyl, C_3-6carbocyclyl, C_3-5heterocyclyl, or —S(O)₂R⁷. In some embodiments, R⁴and R⁵are each independently —H, C_1-6alkyl, or —S(O)₂R⁷. In some embodiments, R⁴and R⁵are each independently —H, methyl, ethyl, propyl, iso-propyl, butyl, sec-butyl, iso-butyl, tert-butyl, or —S(O)₂R⁷. In some embodiments, R⁴and R⁵are each independently —H, methyl, ethyl, propyl, or iso-propyl. In some embodiments, R⁴and R⁵are —H. In some embodiments, R⁴and R⁵are methyl. In some embodiments, R⁴is methyl and R⁵is ethyl. In some embodiments, R⁴is methyl and R⁵is propyl. In some embodiments, R⁴is methyl and R⁵is iso-propyl. In some embodiments, R⁴is H and R⁵is iso-propyl. In some embodiments, R⁴and R⁵are each independently —H or —C(O)N(R¹³)₂.

In other embodiments, R⁴and R⁵attached to the same N atom are taken together with the N atom to which they are attached to form an optionally substituted 4-, 5-, or 6-membered ring heterocycle additionally containing 0-3 heteroatoms selected from —O—, —NH—, —NR⁶—, —S—, and —S(O)₂—. In some embodiments, R⁴and R⁵attached to the same N atom are taken together with the N atom to which they are attached to form a 4-, 5- or 6-membered heterocycle additionally containing 0 or 1 oxygen heteroatom. In other embodiments, R⁴and R⁵attached to the same N atom are taken together with the N atom to which they are attached to form an optionally substituted 4-, 5-, or 6-membered ring heterocycle additionally containing 1 heteroatoms selected from —O—, —NH—, —NR⁶—, —S—, and —S(O)₂—. In other embodiments, R⁴and R⁵attached to the same N atom are taken together with the N atom to which they are attached to form a 4-, 5-, or 6-membered ring heterocycle additionally containing 1 oxygen heteroatom. In other embodiments, R⁴and R⁵attached to the same N atom are taken together with the N atom to which they are attached to form a 4-, 5-, or 6-membered ring.

In some embodiments, le is alkyl, carbocyclyl, optionally substituted aryl, optionally substituted aralkyl, or optionally substituted heterocyclyl. In some embodiments, R⁷is C_1-6alkyl, C_3-6carbocyclyl, or aryl optionally substituted with halo or alkyl. In some embodiments, R⁷is C_1-6alkyl, C_3-6carbocyclyl, or phenyl optionally substituted with halo or alkyl. In some embodiments, le is optionally substituted benzyl. In some embodiments, R⁷is C_3-6carbocyclyl. In some embodiments, R⁷is phenyl. In some embodiments, R⁷is phenyl substituted with 1-4 C_1-6alkyl or fluoro substituents. In some embodiments, R⁷is phenyl substituted with a C_1-6alkyl or fluoro substituent. In some embodiments, R⁷is alkyl, carbocyclyl, aralkyl, or heterocyclyl.

In some embodiments, each R¹³is independently hydrogen, optionally substituted alkyl, haloalkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted aryl, optionally substituted aralkyl, optionally substituted heterocyclyl, or optionally substituted heteroaryl. In some embodiments, each R¹³is independently hydrogen, optionally substituted alkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, or optionally substituted aryl. In some embodiments, each R¹³is independently optionally substituted alkyl, optionally substituted carbocyclyl, optionally substituted heteroalkyl, optionally substituted aryl, or heteroaryl. In some embodiments, each R¹³is independently alkyl or aryl. In some embodiments, each R¹³is independently hydrogen or C_1-6alkyl. In some embodiments, each R¹³is independently hydrogen, methyl, ethyl, propyl, iso-propyl, butyl, iso-butyl, sec-butyl, tert-butyl, pentyl or hexyl. In some embodiments, each R¹³is independently hydrogen, methyl, ethyl, propyl, iso-propyl, butyl, or tert-butyl. In some embodiments, each R¹³is independently hydrogen or methyl. In some embodiments, R¹³is H. In other embodiments, R¹³is alkyl. In other embodiments, R¹³is aryl.

In some embodiments, the substituted steroidal derivative compound described in Formulas provided herein has a structure provided in Table 1.

TABLE 1

Mass,

#
Structure
[M + H]⁺

1

embedded image

500.5

2

embedded image

502.4

3

embedded image

489.4

4

embedded image

518.5

5

embedded image

475.4

6

embedded image

488.5

7

embedded image

516.3

8

embedded image

516.5

9

embedded image

502.4

10

embedded image

488.4

11

embedded image

516.5

12

embedded image

—

13

embedded image

446.3

14

embedded image

506.5

15

embedded image

520.4

16

embedded image

516.5

17

embedded image

476.4

18

embedded image

486.4

19

embedded image

474.4

20

embedded image

529.4

21

embedded image

500.5

22

embedded image

564.3

23

embedded image

432.4

24

embedded image

557.5

25

embedded image

615.3

26

embedded image

418.3

27

embedded image

593.4

28

embedded image

498.4

29

embedded image

526.3

30

embedded image

460.4

31

embedded image

468.4

32

embedded image

—

In some embodiments, the substituted steroidal derivative compound described in Formulas provided herein has a structure provided in Table 2.

TABLE 2

No.
Structure

1a

embedded image

10a

11a

12a

13a

14a

Preparation of the Substituted Steroidal Derivative Compounds

The compounds used in the reactions described herein are made according to organic synthesis techniques known to those skilled in this art, starting from commercially available chemicals and/or from compounds described in the chemical literature. “Commercially available chemicals” are obtained from standard commercial sources including Acros Organics (Pittsburgh, Pa.), Aldrich Chemical (Milwaukee, Wis., including Sigma Chemical and Fluka), Apin Chemicals Ltd. (Milton Park, UK), Avocado Research (Lancashire, U.K.), BDH Inc. (Toronto, Canada), Bionet (Cornwall, U.K.), Chemservice Inc. (West Chester, Pa.), Crescent Chemical Co. (Hauppauge, N.Y.), Eastman Organic Chemicals, Eastman Kodak Company (Rochester, N.Y.), Fisher Scientific Co. (Pittsburgh, Pa.), Fisons Chemicals (Leicestershire, UK), Frontier Scientific (Logan, Utah), ICN Biomedicals, Inc. (Costa Mesa, Ca.), Key Organics (Cornwall, U.K.), Lancaster Synthesis (Windham, N.H.), Maybridge Chemical Co. Ltd. (Cornwall, U.K.), Parish Chemical Co. (Orem, Utah), Pfaltz & Bauer, Inc. (Waterbury, Conn.), Polyorganix (Houston, Tex.), Pierce Chemical Co. (Rockford, Ill.), Riedel de Haen AG (Hanover, Germany), Spectrum Quality Product, Inc. (New Brunswick, N.J.), TCI America (Portland, Oreg., Trans World Chemicals, Inc. (Rockville, Md.), and Wako Chemicals USA, Inc. (Richmond, Va.).

Suitable reference books and treatise that detail the synthesis of reactants useful in the preparation of compounds described herein, or provide references to articles that describe the preparation, include for example, “Synthetic Organic Chemistry”, John Wiley & Sons, Inc., New York; S. R. Sandler et al., “Organic Functional Group Preparations,” 2nd Ed., Academic Press, New York, 1983; H. O. House, “Modern Synthetic Reactions”, 2nd Ed., W. A. Benjamin, Inc. Menlo Park, Calif. 1972; T. L. Gilchrist, “Heterocyclic Chemistry”, 2nd Ed., John Wiley & Sons, New York, 1992; J. March, “Advanced Organic Chemistry: Reactions, Mechanisms and Structure”, 4th Ed., Wiley-Interscience, New York, 1992. Additional suitable reference books and treatise that detail the synthesis of reactants useful in the preparation of compounds described herein, or provide references to articles that describe the preparation, include for example, Fuhrhop, J. and Penzlin G “Organic Synthesis: Concepts, Methods, Starting Materials”, Second, Revised and Enlarged Edition (1994) John Wiley & Sons ISBN: 3-527-29074-5; Hoffman, R.V. “Organic Chemistry, An Intermediate Text” (1996) Oxford University Press, ISBN 0-19-509618-5; Larock, R. C. “Comprehensive Organic Transformations: A Guide to Functional Group Preparations” 2nd Edition (1999) Wiley-VCH, ISBN: 0-471-19031-4; March, J. “Advanced Organic Chemistry: Reactions, Mechanisms, and Structure” 4th Edition (1992) John Wiley & Sons, ISBN: 0-471-60180-2; Otera, J. (editor) “Modern Carbonyl Chemistry” (2000) Wiley-VCH, ISBN: 3-527-29871-1; Patai, S. “Patai's 1992 Guide to the Chemistry of Functional Groups” (1992) Interscience ISBN: 0-471-93022-9; Solomons, T. W. G. “Organic Chemistry” 7th Edition (2000) John Wiley & Sons, ISBN: 0-471-19095-0; Stowell, J. C., “Intermediate Organic Chemistry” 2nd Edition (1993) Wiley-Interscience, ISBN: 0-471-57456-2; “Industrial Organic Chemicals: Starting Materials and Intermediates: An Ullmann's Encyclopedia” (1999) John Wiley & Sons, ISBN: 3-527-29645-X, in 8 volumes; “Organic Reactions” (1942-2000) John Wiley & Sons, in over 55 volumes; and “Chemistry of Functional Groups” John Wiley & Sons, in 73 volumes.

Specific and analogous reactants are optionally identified through the indices of known chemicals prepared by the Chemical Abstract Service of the American Chemical Society, which are available in most public and university libraries, as well as through on-line databases (contact the American Chemical Society, Washington, D.C. for more details). Chemicals that are known but not commercially available in catalogs are optionally prepared by custom chemical synthesis houses, where many of the standard chemical supply houses (e.g., those listed above) provide custom synthesis services. A reference for the preparation and selection of pharmaceutical salts of the substituted steroidal derivative compounds described herein is P. H. Stahl & C. G. Wermuth “Handbook of Pharmaceutical Salts”, Verlag Helvetica Chimica Acta, Zurich, 2002.

Pharmaceutical Compositions of the Substituted Steroidal Derivative Compounds

In certain embodiments, the substituted steroidal derivative compound as described herein is administered as a pure chemical. In other embodiments, the substituted steroidal derivative compound described herein is combined with a pharmaceutically suitable or acceptable carrier (also referred to herein as a pharmaceutically suitable (or acceptable) excipient, physiologically suitable (or acceptable) excipient, or physiologically suitable (or acceptable) carrier) selected on the basis of a chosen route of administration and standard pharmaceutical practice as described, for example, in Remington: The Science and Practice of Pharmacy (Gennaro, 21^stEd. Mack Pub. Co., Easton, Pa. (2005)).

Provided herein is a pharmaceutical composition comprising at least one substituted steroidal derivative compound, or a stereoisomer, pharmaceutically acceptable salt, hydrate, solvate, or N-oxide thereof, together with one or more pharmaceutically acceptable carriers. The carrier(s) (or excipient(s)) is acceptable or suitable if the carrier is compatible with the other ingredients of the composition and not deleterious to the recipient (i.e., the subject) of the composition.

One embodiment provides a pharmaceutical composition comprising a compound of any of the Formulas provided herein, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient.

In certain embodiments, the substituted steroidal derivative compound as described by any of the Formulas provided herein is substantially pure, in that it contains less than about 5%, or less than about 1%, or less than about 0.1%, of other organic small molecules, such as unreacted intermediates or synthesis by-products that are created, for example, in one or more of the steps of a synthesis method.

Suitable oral dosage forms include, for example, tablets, pills, sachets, or capsules of hard or soft gelatin, methylcellulose or of another suitable material easily dissolved in the digestive tract. In some embodiments, suitable nontoxic solid carriers are used which include, for example, pharmaceutical grades of mannitol, lactose, starch, magnesium stearate, sodium saccharin, talcum, cellulose, glucose, sucrose, magnesium carbonate, and the like. (See, e.g., Remington: The Science and Practice of Pharmacy (Gennaro, 21^stEd. Mack Pub. Co., Easton, Pa. (2005)).

The dose of the composition comprising at least one substituted steroidal derivative compound as described herein differ, depending upon the patient's (e.g., human) condition, that is, stage of the disease, general health status, age, and other factors.

Pharmaceutical compositions are administered in a manner appropriate to the disease to be treated (or prevented). An appropriate dose and a suitable duration and frequency of administration will be determined by such factors as the condition of the patient, the type and severity of the patient's disease, the particular form of the active ingredient, and the method of administration. In general, an appropriate dose and treatment regimen provides the composition(s) in an amount sufficient to provide therapeutic and/or prophylactic benefit (e.g., an improved clinical outcome, such as more frequent complete or partial remissions, or longer disease-free and/or overall survival, or a lessening of symptom severity. Optimal doses are generally determined using experimental models and/or clinical trials. The optimal dose depends upon the body mass, weight, or blood volume of the patient.

Oral doses typically range from about 1.0 mg to about 1000 mg, one to four times, or more, per day.

Use of the Substituted Steroidal Derivative Compounds
Glucocorticoid Receptor Modulators

Mifepristone is a non-selective modulator of several nuclear receptors. Mifepristone has been referred to as a GR antagonist, a progesterone receptor (PR) antagonist, a GR partial agonist, an androgen receptor (AR) antagonist and an AR partial agonist in the scientific literature. The activity observed at multiple hormone receptors leads to various undesirable side effects and in some instances, the promotion of cancer. Thus, AR agonism is an undesirable feature for GR antagonists used in the treatment of cancer (e.g., AR positive or AR dependent cancers including “castration resistant” prostate cancer (CRPC), breast cancer, or ovarian cancer). Antagonists of GR that have minimized binding to other hormone receptors, such as the androgen receptor (AR), are needed to effectively treat the diseases described herein with reduced side effects.

Some embodiments provided herein describe compounds that are modulators of glucocorticoid receptors (GR). In some embodiments, the compounds alter the level and/or activity of GR. In some embodiments, the compounds described herein are GR inhibitors. In some embodiments, the GR inhibitors are GR antagonists. In some instances, glucocorticoid receptor antagonists bind to the receptor and prevent glucocorticoid receptor agonists from binding and eliciting GR mediated events, including transcription. Thus, in some embodiments, the compounds described herein inhibit GR transcriptional activation activity. In some embodiments, the compounds described herein are selective GR antagonists. In some embodiments, the compounds described herein are not GR agonists. In some embodiments, the compounds described herein are not GR partial agonists. In some embodiments, the GR inhibitors lessen cortisol activity in cells and make secondary therapeutic agents more effective.

GR antagonists are useful for treating or preventing weight gain (e.g., Olanzapine induced weight gain), uterine fibrosis, alcoholism, alcohol abuse disorders, cocaine dependence, bipolar depression, adrenal hypercortisolism, post-traumatic stress disorder, anxiety disorders, mood disorders, hyperglycemia, and to induce abortion.

In some embodiments, the GR inhibitors described herein are also androgen receptor (AR) signaling inhibitors. In certain embodiments, the AR signaling inhibitors are AR antagonists. In some instances, AR antagonists bind to AR and prevent AR agonists from binding and eliciting AR mediated events, including transcription. In other embodiments, the GR inhibitors are not androgen receptor (AR) signaling inhibitors. In these instances, the GR inhibitors do not significantly activate AR levels and/or activity. In some embodiments, the GR inhibitors are not AR agonists.

In some embodiments, the GR inhibitors described herein have minimized binding to the androgen receptor (AR). In some embodiments, the compounds described herein are not AR agonists. In some embodiments, the compounds described herein are not partial AR agonists. In some embodiments, the compounds described herein have minimized partial AR agonism compared to mifepristone.

In some embodiments, the GR inhibitors described herein are not partial AR agonists or partial GR agonists.

In some embodiments, the GR inhibitors described herein do not modulate progesterone receptors. In some embodiments, the GR inhibitors described herein are not progesterone receptor (PR) inhibitors. In these instances, the GR inhibitors do not significantly activate PR levels and/or activity. In some embodiments, the GR inhibitors are not PR agonists. In some embodiments, the GR inhibitors are not PR partial agonists. In some embodiments, the GR inhibitors are not PR antagonists.

In some embodiments, the GR inhibitors (e.g., GR antagonists) are selective inhibitors. In some embodiments, use of the GR inhibitors in a patient does not cause or result in vaginal bleeding, cramping, nausea, vomiting, diarrhea, dizziness, back pain, weakness, tiredness, or combinations thereof. In certain embodiments, use of the GR inhibitors in a patient does not cause or result in vaginal bleeding. In certain embodiments, use of the GR inhibitors in a patient does not cause or result in cramping. In some embodiments, use of the GR inhibitors in a patient does not cause or result in allergic reactions, low blood pressure, loss of consciousness, shortness of breath, rapid heartbeat, or combinations thereof.

Methods of Treatment
Cancer

One embodiment provides a method of treating cancer in a subject in need thereof, comprising administering to the subject a compound of any of the Formulas provided herein, or a pharmaceutically acceptable salt thereof. In some embodiments, a GR inhibitor described herein is used in combination with a second therapeutic agent (e.g., an anti-cancer agent) for treating cancer. In some embodiments, the combination of the GR inhibitor with the second therapeutic agent (e.g., an anti-cancer agent) provides a more effective initial therapy for treating cancer compared to the second therapeutic agent (e.g., an anti-cancer agent) administered alone. In some embodiments, a GR inhibitor described herein is used in combination with one or more additional therapeutic agents (e.g., anti-cancer agents) for treating cancer. In some embodiments, the combination of the GR inhibitor with the one or more additional therapeutic agents (e.g., an anti-cancer agents) provides a more effective initial therapy for treating cancer compared to the one or more therapeutic agents (e.g., an anti-cancer agents) administered alone.

In some embodiments, the cancer is chemoresistant cancer, radio resistant cancer, or refractory cancer. In some embodiments, the cancer is relapsed cancer, persistent cancer, or recurrent cancer. Another embodiment provided herein describes a method of reducing incidences of cancer recurrence. Also provided here in some embodiments, is a method for treating a chemo-resistant cancer.

Prostate Cancer

Prostate cancer is the second most common cause of cancer death in men in the United States, and approximately one in every six American men will be diagnosed with the disease during his lifetime. Treatment aimed at eradicating the tumor is unsuccessful in 30% of men.

One embodiment provides a method of treating prostate cancer in a subject in need thereof, comprising administering to the subject a compound of any of the Formulas provided herein, or a pharmaceutically acceptable salt thereof. In some embodiments, a GR inhibitor described herein is used in combination with a second therapeutic agent (e.g., an anti-cancer agent) for treating prostate cancer. In some embodiments, the combination of the GR inhibitor with the second therapeutic agent (e.g., an anti-cancer agent) provides a more effective initial therapy for treating prostate cancer compared to the second therapeutic agent (e.g., an anti-cancer agent) administered alone. In some embodiments, a GR inhibitor described herein is used in combination with one or more additional therapeutic agents (e.g., anti-cancer agents) for treating prostate cancer. In some embodiments, the combination of the GR inhibitor with the one or more additional therapeutic agents (e.g., an anti-cancer agents) provides a more effective initial therapy for treating prostate cancer compared to the one or more therapeutic agents (e.g., an anti-cancer agents) administered alone.

In some embodiments, the prostate cancer is chemoresistant cancer, radio resistant cancer, antiandrogen resistant, or refractory cancer. In some embodiments, the prostate cancer is relapsed cancer, persistent cancer, or recurrent cancer.

In some embodiments, the prostate cancer is acinar adenocarcinoma, atrophic carcinoma, foamy carcinoma, colloid carcinoma, or signet ring carcinoma. In some embodiments, the prostate cancer is ductal adenomcarcinoma, transitional cell cancer, urothelial cancer, squamous cell cancer, carcinoid cancer, small cell cancer, sarcoma cancer, or sarcomatoid cancer. In some embodiments, the prostate cancer is metastatic castration-resistant prostate cancer, doubly-resistant prostate cancer, castration-resistant prostate cancer, hormone-resistant prostate cancer, androgen-independent, or androgen-refractory cancer.

In some instances, antiandrogens are useful for the treatment of prostate cancer during its early stages. In some instances, prostate cancer cells depend on androgen receptor (AR) for their proliferation and survival. Some prostate cancer patients are physically castrated or chemically castrated by treatment with agents that block production of testosterone (e.g. GnRH agonists), alone or in combination with antiandrogens, which antagonize effects of any residual testosterone.

In some instances, prostate cancer advances to a hormone-refractory state in which the disease progresses despite continued androgen ablation or antiandrogen therapy. The hormone-refractory state to which most patients eventually progresses in the presence of continued androgen ablation or anti-androgen therapy is known as “castration resistant” prostate cancer (CRPC). CRPC is associated with an overexpression of AR. AR is expressed in most prostate cancer cells and overexpression of AR is necessary and sufficient for androgen-independent growth of prostate cancer cells. Failure in hormonal therapy, resulting from development of androgen-independent growth, is an obstacle for successful management of advanced prostate cancer.

While a small minority of CRPC does bypass the requirement for AR signaling, the vast majority of CRPC, though frequently termed “androgen independent prostate cancer” or “hormone refractory prostate cancer,” retains its lineage dependence on AR signaling

Recently approved therapies that target androgen receptor (AR) signaling such as abiraterone and enzalutamide have been utilized for treating CRPC. Despite these successes, sustained response with these agents is limited by acquired resistance which typically develops within 6-12 months. Doubly resistant prostate cancer is characterized in that tumor cells have become castration resistant and overexpress AR, a hallmark of CRPC. However, cells remain resistant when treated with second generation antiandrogens. Doubly resistant prostate cancer cells are characterized by a lack of effectiveness of second generation antiandrogens in inhibiting tumor growth.

As discussed above, resistant prostate cancer (e.g., doubly resistant and castration resistant prostate cancers) occurs when cancer cells overexpress androgen receptors (AR). AR target gene expression is inhibited when the cells are treated with a second generation antiandrogen. In some instances, increased signaling through the glucocorticoid receptor (GR) compensates for inhibition of androgen receptor signaling in resistant prostate cancer. Double resistant prostate cancer develops when expression of a subset of those AR target genes is restored. In some instances, GR activation is responsible for this target gene activation. In some embodiments, GR transcription is activated in patients susceptible to or suffering from resistant prostate cancer (e.g., doubly resistant and castration resistant prostate cancers). In some instances, GR upregulation in cancer cells confers resistance to antiandrogens.

Some embodiments provided herein describe the use of the GR inhibitors for treating prostate cancer in a subject in need thereof, including doubly resistant prostate cancer and castration resistant prostate cancer. In some embodiments, the subject in need has elevated tumor GR expression. In some embodiments, the GR inhibitor is also an AR signaling inhibitor or antiandrogen.

In some embodiments, the GR inhibitor is used in combination with a second therapeutic agent. In some embodiments, the GR inhibitor is used in combination with one or more additional therapeutic agents. In some embodiments, the second or additional agent is an anti-cancer agent. In certain embodiments, the anti-cancer agent is useful for AR positive or AR negative prostate cancer.

In some embodiments, the second or additional agent is an AR signaling inhibitor or antiandrogen. In certain embodiments, the AR signaling inhibitor is an AR antagonist. In some embodiments, the second or additional therapeutic agent is selected from finasteride, dutasteride, alfatradiol, cyproterone acetate, spironolactone, danazol, gestrinone, ketoconazole, abiraterone acetate, enzalutamide, ARN-509, danazol, gestrinone, danazol, simvastatin, aminoglutethimide, atorvastatin, simvastatin, progesterone, cyproterone acetate, medroxyprogesterone acetate, megestrol acetate, chlormadinone acetate, spironolactone, drospirenone, estradiol, ethinyl estradiol, diethylstilbestrol, conjugated equine estrogens, buserelin, deslorelin, gonadorelin, goserelin, histrelin, leuprorelin, nafarelin, triptorelin, abarelix, cetrorelix, degarelix, ganirelix, or any combinations or any salts thereof. In some embodiments, the second or additional therapeutic agent is selected from flutamide, nilutamide, bicalutamide, enzalutamide, apalutamide, cyproterone acetate, megestrol acetate, chlormadinone acetate, spironolactone, canrenone, drospirenone, ketoconazole, topilutamide, cimetidine, or any combinations or any salts thereof. In some embodiments, the AR signaling inhibitor is 3,3′-diindolylmethane (DIM), abiraterone acetate, ARN-509, bexlosteride, bicalutamide, dutasteride, epristeride, enzalutamide, finasteride, flutamide, izonsteride, ketoconazole, N-butylbenzene-sulfonamide, nilutamide, megestrol, steroidal antiandrogens, turosteride, or any combinations thereof. In some embodiments, the AR signaling inhibitor is flutamide, nilutamide, bicalutamide, or megestrol. In some embodiments, the AR signaling inhibitor is ARN-509. In other embodiments, the AR signaling inhibitor is enzalutamide.

In some embodiments, the anti-cancer agent is mitoxantrone, estramustine, etoposide, vinblastine, carboplatin, vinorelbine, paclitaxel, daunomycin, darubicin, epirubicin, docetaxel, cabazitaxel, or doxorubicin. In some embodiments, the anti-cancer agent is paclitaxel, daunomycin, darubicin, epirubicin, docetaxel, cabazitaxel, or doxorubicin. In certain embodiments, the anti-cancer agent is docetaxel.

Breast Cancer

Breast cancer is the second leading cause of cancer among women in the United States. Triple-negative breast cancers are among the most aggressive and difficult to treat of all the breast cancer types. Triple-negative breast cancer is a form of the disease in which the three receptors that fuel most breast cancer growth estrogen, progesterone and the HER-2—are not present. Because the tumor cells lack these receptors, treatments that target estrogen, progesterone and HER-2 are ineffective. Approximately 40,000 women are diagnosed with triple-negative breast cancer each year. It is estimated that more than half of these women's tumor cells express significant amounts of GR.

In some instances, GR expression is associated with a poor prognosis in estrogen receptor (ER)-negative early stage breast cancer. In some instances, GR activation in triple-negative breast cancer cells initiates an anti-apoptotic gene expression profile that is associated with inhibiting chemotherapy-induced tumor cell death. GR activity in these cancer cells correlate with chemotherapy resistance and increased recurrence of cancer.

Provided herein in some embodiments are methods of treating breast cancer, the method comprising administering to a subject in need thereof a compound of any of the Formulas provided herein, or a pharmaceutically acceptable salt thereof. In some embodiments, a GR inhibitor described herein is used in combination with a second therapeutic agent (e.g., a chemotherapeutic agent) for treating breast cancer. In some embodiments, the combination of the GR inhibitor with the second therapeutic agent (e.g., a chemotherapeutic agent) provides a more effective initial therapy for treating breast cancer compared to the second therapeutic agent (e.g., a chemotherapeutic agent) administered alone. In some embodiments, a GR inhibitor described herein is used in combination with one or more additional therapeutic agents (e.g., anti-cancer agents) for treating breast cancer. In some embodiments, the combination of the GR inhibitor with the one or more additional therapeutic agents (e.g., an anti-cancer agents) provides a more effective initial therapy for treating breast cancer compared to the one or more therapeutic agents (e.g., an anti-cancer agents) administered alone.

In some embodiments, the breast cancer is chemoresistant cancer, radio resistant cancer, or refractory cancer. In some embodiments, the breast cancer is relapsed cancer, persistent cancer, or recurrent cancer. Breast cancers may include, but are not limited to, ductal carcinoma, invasive ductal carcinoma, tubular carcinoma of the breast, medullary carcinoma of the breast, mecinous carcinoma of the breast, papillary carcinoma of the breast, cribriform carcinoma of the breast, invasive lobular carcinoma, inflammatory breast cancer, lobular carcinoma in situ, male breast cancer, Paget disease of the nipple, phyllodes tumor of the breast, recurrent and metastatic breast cancer, triple-negative breast cancer, or combinations thereof.

In some embodiments, the breast cancer is recurrent and metastatic breast cancer, triple-negative breast cancer, or combinations thereof. In some embodiments, the breast cancer is chemoresistant triple-negative breast cancer or estrogen receptor (ER) negative breast cancer. In some embodiments, the breast cancer is chemoresistant triple-negative breast cancer. In some embodiments, the breast cancer is estrogen receptor (ER) negative breast cancer. In some embodiments, the breast cancer is GR+ triple-negative breast cancer. In some embodiments, the breast cancer is GR+ estrogen receptor (ER) negative breast cancer.

Some embodiments provided herein describe the use of GR inhibitors for treating breast cancer in a patient, including triple negative breast cancer or ER negative breast cancer. In some embodiments, GR inhibitors inhibit the anti-apoptotic signaling pathways of GR and increase the cytotoxic efficiency of secondary chemotherapeutic agents. In some embodiments, the GR inhibitors described herein enhance the efficacy of chemotherapy in breast cancer patients, such as triple negative breast cancer patients. In some embodiments, the breast cancer patient has elevated tumor GR expression.

In some embodiments, a GR inhibitor described herein is used in combination with a second therapeutic agent, such as chemotherapy or immunotherapy. In some embodiments, a GR inhibitor described herein is used in combination with one or more additional therapeutic agents. In some embodiments, the second or additional chemotherapeutic agent is cisplatin, carboplatin, cyclophosphamide, capecitabine, gemcitabine, paclitaxel, nab-paclitaxel, altretamine, docetaxel, epirubicin, melphalan, methotrexate, mitoxantrone, ixabepilone, ifosfamide, irinotecan, eribulin, etoposide, doxorubicin, liposomal doxorubicin, camptothecin, pemetrexed, topotecan, vinorelbine, daunorubicin, fluorouracil, mitomycin, thiotepa, vincristine, everolimus, veliparib, glembatumumab vedotin, pertuzumab, trastuzumab, or any combinations or any salts thereof. In some embodiments, the second or additional therapeutic agent is an anti-PD-L1 agent. In certain embodiments, the anti-PD-L1 agent is MPDL3280A or avelumab. In some embodiments, the second or additional therapeutic agent is an anti-PD1 agent. In certain embodiments, the anti-PD1 agent is nivolumab or permbrolizumab.

Some embodiments provided herein describe methods of treating estrogen positive breast cancer. In some instances, estrogen positive breast cancer patients become resistant to estrogen receptor modulators. In some embodiments, the GR inhibitors described herein enhance the efficacy of estrogen receptor modulators in estrogen positive breast cancer patients. In some embodiments, the breast cancer patient has elevated tumor GR expression. In some embodiments, a GR inhibitor described herein is used in combination with an estrogen receptor modulator. In some embodiments, the estrogen receptor modulator is tamoxifen, raloxifene, toremifene, tibolone, fulvestrant, lasofoxifene, clomifene, ormeloxifene, or ospemifene. In some embodiments, the estrogen receptor modulator is tamoxifen, raloxifene, toremifene, tibolone, or fulvestrant. In some embodiments, the estrogen receptor modulator is tamoxifen, raloxifene, or toremifene. In certain embodiments, the estrogen receptor modulator is tamoxifen.

Ovarian Cancer

Ovarian cancer is the leading cause of death from gynecologic malignancies. Some ovarian cancers (e.g., high grade serous ovarian cancer) are initially sensitive to platinum-based therapy, but relapse rates remain high.

One embodiment provides a method of treating ovarian cancer in a patient in need thereof, comprising administering to the patient a compound of any of the Formulas provided herein, or a pharmaceutically acceptable salt thereof. In some embodiments, the patient has elevated tumor GR expression. In some embodiments, a GR inhibitor described herein is used in combination with a second therapeutic agent (e.g., a chemotherapeutic agent) for treating ovarian cancer. In some embodiments, the combination of the GR inhibitor with the second therapeutic agent (e.g., a chemotherapeutic agent) provides a more effective initial therapy for treating ovarian cancer compared to the second therapeutic agent (e.g., a chemotherapeutic agent) administered alone. In some embodiments, a GR inhibitor described herein is used in combination with one or more additional therapeutic agents (e.g., anti-cancer agents) for treating ovarian cancer. In some embodiments, the combination of the GR inhibitor with the one or more additional therapeutic agents (e.g., an anti-cancer agents) provides a more effective initial therapy for treating ovarian cancer compared to the one or more therapeutic agents (e.g., an anti-cancer agents) administered alone.

In some instances, GR activation increases resistance to chemotherapy in ovarian cancer (e.g., high-grade serous ovarian cancer). In some instances, GR activation significantly inhibits chemotherapy induced apoptosis in ovarian cancer cells. Provided herein in some embodiments are methods of treating ovarian cancer in a subject, the method comprising treating the subject with a GR inhibitor (e.g., GR antagonist) to improve sensitivity to chemotherapy. In some embodiments, the ovarian cancer has become resistant to chemotherapy. In some embodiments, the ovarian cancer cells are resistant to cisplatin, paclitaxel, carboplatin, gemcitabine, alone or in combination. In some embodiments, the GR inhibitor or antagonist reverses the cell survival effect.

Ovarian cancers may include, but are not limited to, epithelial ovarian cancers, such as serous epithelial ovarian cancer, endometrioid epithelial ovarian cancer, clear cell epithelial ovarian cancer, mucinous epithelial ovarian cancer, undifferentiated or unclassifiable epithelial ovarian cancer, refractory ovarian cancer, sex cord-stromal tumors, Sertoli and Sertoli-Leydig cell tumors, germ cell tumors, such as dysgerminoma and nondysgerminomatous tumors, Brenner tumors, primary peritoneal carcinoma, fallopian tube cancer, or combinations thereof.

In some embodiments, the GR inhibitor is used in combination with at least a second therapeutic agent, such as chemotherapy or immunotherapy. In some embodiments, the GR inhibitor is used in combination with one or more additional therapeutic agents. In some embodiments, the second or additional chemotherapeutic agent is cisplatin, carboplatin, cyclophosphamide, capecitabine, gemcitabine, paclitaxel, nab-paclitaxel, altretamine, docetaxel, epirubicin, melphalan, methotrexate, mitoxantrone, ixabepilone, ifosfamide, irinotecan, eribulin, etoposide, doxorubicin, liposomal doxorubicin, camptothecin, pemetrexed, topotecan, vinorelbine, daunorubicin, fluorouracil, mitomycin, thiotepa, vincristine, everolimus, veliparib, glembatumumab vedotin, pertuzumab, trastuzumab, or any combinations or any salts thereof. In some embodiments, the second or additional chemotherapeutic agent is gemcitabine. In some embodiments, the second or additional chemotherapeutic agent is carboplatin. In some embodiments, the second or additional chemotherapeutic agent is cisplatin. In some embodiments, the second or additional agent is paclitaxel. In some embodiments, the GR inhibitor is used in combination with gemcitabine and carboplatin. In some embodiments, the GR inhibitor is used in combination with carboplatin and cisplatin. In some embodiments, the second or additional therapeutic agent is an anti-PD-L1 agent. In certain embodiments, the anti-PD-L1 agent is MPDL3280A or avelumab. In some embodiments, the second or additional therapeutic agent is an anti-PD1 agent. In certain embodiments, the anti-PD1 agent is nivolumab or permbrolizumab.

Non-Small Cell Lung Cancer

One embodiment provides a method of treating non-small cell lung cancer (NSCLC) in a patient in need thereof, comprising administering to the patient a compound of any of the Formulas provided herein, or a pharmaceutically acceptable salt thereof. In some embodiments, the patient has elevated tumor GR expression. In some embodiments, a GR inhibitor described herein is used in combination with a second therapeutic agent (e.g., a chemotherapeutic agent) for treating NSCLC. In some embodiments, the combination of the GR inhibitor with the second therapeutic agent (e.g., a chemotherapeutic agent) provides a more effective initial therapy for treating NSCLC compared to the second therapeutic agent (e.g., a chemotherapeutic agent) administered alone. In some embodiments, a GR inhibitor described herein is used in combination with one or more additional therapeutic agents (e.g., anti-cancer agents) for treating NSCLC. In some embodiments, the combination of the GR inhibitor with the one or more additional therapeutic agents (e.g., an anti-cancer agents) provides a more effective initial therapy for treating NSCLC compared to the one or more therapeutic agents (e.g., an anti-cancer agents) administered alone.

In some embodiments, the GR inhibitor is used in combination with at least a second therapeutic agent, such as a chemotherapeutic agent or immunotherapy. In some embodiments, the GR inhibitor is used in combination with one or more additional therapeutic agents. In some embodiments, the second or additional chemotherapeutic agent is cisplatin, carboplatin, cyclophosphamide, capecitabine, gemcitabine, paclitaxel, nab-paclitaxel, altretamine, docetaxel, epirubicin, melphalan, methotrexate, mitoxantrone, ixabepilone, ifosfamide, irinotecan, eribulin, etoposide, doxorubicin, liposomal doxorubicin, camptothecin, pemetrexed, topotecan, vinorelbine, vinblastine, daunorubicin, fluorouracil, mitomycin, thiotepa, vincristine, everolimus, veliparib, glembatumumab vedotin, pertuzumab, trastuzumab, or any combinations or any salts thereof. In some embodiments, the second or additional chemotherapeutic agent is gemcitabine. In some embodiments, the second or additional chemotherapeutic agent is carboplatin. In some embodiments, the second or additional chemotherapeutic agent is cisplatin. In some embodiments, the second or additional agent is paclitaxel. In some embodiments, the GR inhibitor is used in combination with gemcitabine and carboplatin. In some embodiments, the GR inhibitor is used in combination with carboplatin and cisplatin. In some embodiments, the second or additional therapeutic agent is an anti-PD-L1 agent. In certain embodiments, the anti-PD-L1 agent is MPDL3280A or avelumab. In some embodiments, the second or additional therapeutic agent is an anti-PD1 agent. In certain embodiments, the anti-PD1 agent is nivolumab or permbrolizumab.

Hypercortisolism/Cushing's Disease

One embodiment provides a method of treating hypercortisolism or Cushing's disease in a patient in need thereof, comprising administering to the patient a compound of any of the Formulas provided herein, or a pharmaceutically acceptable salt thereof.

Types of Cushing's disease include, but are not limited to, recurrent Cushing's disease, refractory Cushing's disease, persistent Cushing's disease, endogenous Cushing's disease, spontaneous hypercortisolism, Adrenocorticotropic hormone dependent, Adrenocorticotropic hormone independent, or combinations thereof.

Causes of hypercortisolism may include, but are not limited to, prolonged exposure to cortisol, a tumor that produces excessive cortisol, a tumor that results in the excess production of cortisol, or combinations thereof.

Other embodiments and uses will be apparent to one skilled in the art in light of the present disclosures. The following examples are provided merely as illustrative of various embodiments and shall not be construed to limit the invention in any way.

EXAMPLES
I. Chemical Synthesis

Unless otherwise noted, reagents and solvents were used as received from commercial suppliers. Anhydrous solvents and oven-dried glassware were used for synthetic transformations sensitive to moisture and/or oxygen. Yields were not optimized. Reaction times are approximate and were not optimized. Column chromatography and thin layer chromatography (TLC) were performed on silica gel unless otherwise noted.

Example 1
Compound 17. (8S,9R,10R,11S,13S,14S,17S)-11-(4-(Dimethylamino)phenyl)-17-hydroxy-17-(3-methoxyprop-1-yn-1-yl)-10,13-dimethyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one

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STEP A. (8′S,9′S,10′R,13′S,14′S)-10′,13′-Dimethyl-1′,2′,4′,7′,8′,9′,10′,12′,13′,14′,15′,16′-dodecahydro-11′H-dispiro[[1,3]dioxolane-2,3′-cyclopenta[a]phenanthrene-17′,2″-[1,3]dioxolan]-11′-one

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Adrenosterone (50.2 g, 1 eq) was dissolved in 390 ml DCM with 91 g of trimethylorthoformate (5.2 eq) and 119 g of ethylene glycol (11.5 eq). Toluenesulfonic acid (1.9 g, 0.06 eq) was added. The reaction was heated to 40° C. for 18 h. The dark colored solution was quenched with 4 ml pyridine. The reaction was concentrated to remove solvents. The residue was taken up in DCM and washed with water. The organic layer was dried with MgSO₄, filtered and concentrated to give 74.3 g of a mushy solid. The solid was purified to give 42.2 g (65%) of the title compound. m/z (ESI, +ve ion) 389.3 (M+H)⁺.

STEP B. (8′S,9′S,10′R,13′R,14′S)-10′,13′-dimethyl-1′,4′,7′,8′,9′,10′,13′,14′,15′,16′-decahydro-2′H-dispiro[[1,3]dioxolane-2,3′-cyclopenta[a]phenanthrene-17′,2″-[1,3]dioxolan]-11′-yl 1,1,2,2,3,3,4,4,4-nonafluorobutane-1-sulfonate

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Lithium diisopropylamide solution was prepared as follows. In an oven-dried 500 mL flask a solution of 125 mL THF and anhydrous diisopropylamine (5.77 mL, 4.1 eq) was cooled in a dry ice-acetone bath. N-butyl lithium (25.1 mL, 4 eq, 1.6 M in hexanes) was added slowly and the reaction was stirred for 25 min.

A separate flask was charged with the starting bis-ketal (Step A) (3.9 g, 1 eq), azeotroped with toluene, dried under vacuum, flushed with Ar and kept under an Ar balloon. 40 mL of THF was added to dissolve the bis-ketal. This solution was added slowly to the freshly prepared LDA solution maintained at −78° C. The reaction was stirred for 30 min, then perfluorobutanesulfonyl fluoride (5.4 mL, 3 eq) was added. The reaction was maintained in the cold bath for another hour. The bath was removed and the mixture allowed to stir at rt overnight. After 24 h, additional 2.5 mL perfluorobutanesulfonyl fluoride was added and the reaction was stirred overnight again. Saturated aq. NH₄Cl was added to the reaction, followed by extraction with EtOAc. The organic layer was washed with brine and dried with MgSO₄. After concentration under vacuum, the residue was purified by silica gel chromatography to give the desired product as a light yellow solid (3.7 g, 55%). m/z (ESI, +ve ion) 671.2 (M+H)⁺.

Step C. tert-Butyl (4-((8′S,9′S,10′R,13′R,14′S)-10′,13′-dimethyl-1′,4′,7′,8′,9′,10′,13′,14′,15′,16′-decahydro-2′H-dispiro[[1,3]dioxolane-2,3′-cyclopenta[a]phenanthrene-17′,2″-[1,3]dioxolan]-11′-yl)phenyl)(methyl)carbamate

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A flask was charged with (8′S,9′S,10′R,13′R,14′S)-10′,13′-dimethyl-1′,4′,7′,8′,9′,10′,13′,14′,15′,16′-decahydro-2′H-dispiro[[1,3]dioxolane-2,3′-cyclopenta[a]phenanthrene-17′,2″-[1,3]dioxolan]-11′-yl 1,1,2,2,3,3,4,4,4-nonafluorobutane-1-sulfonate (Step B) (1.4 g, 1 eq), (4-((tert-butoxycarbonyl)(methyl)amino)phenyl)boronic acid (5.2 g, 10 eq), lithium chloride (177 mg, 2 eq), and Pd(PPh₃)₄(193 mg, 0.08 eq). Then toluene (36 mL), ethanol (18 mL), and Na₂CO₃(2M, 7.8 mL, 7.5 eq) were added successively and the reaction mixture was degassed with argon. After the reaction was refluxed for 42 h, it was cooled to rt, quenched with aq. NaHCO₃solution, and extracted with EtOAc. The organic layer was separated, washed with saturated brine solution, dried with MgSO₄, filtered and concentrated to dryness in vacuo. Purification by flash silica gel column chromatography provided the title compound (1.1 g, 92%) as a white solid.

Step D. tert-Butyl (4-((4a′R,5a′S,6a′S,6b′S,9a′R,11a′R,11b′R)-9a′,11b′-dimethyl-1′,5a′,6′,6a′,6b′,7′,8′,9a′,11a′,11b′-decahydro-2′H,4′H-dispiro[[1,3]dioxolane-2,3′-cyclopenta[1,2]phenanthro[8a,9-b]oxirene-9′,2″-[1,3]dioxolan]-11′-yl)phenyl)(methyl)carbamate

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To a solution of tert-butyl (4-((8′S,9′S,10′R,13′R,14′S)-10′,13′-dimethyl-1′,4′,7′,8′,9′,10′,13′,14′,15′,16′-decahydro-2′H-dispiro[[1,3]dioxolane-2,3′-cyclopenta[a]phenanthrene-17′,2″-[1,3]dioxolan]-11′-yl)phenyl)(methyl)carbamate (Step C) (520 mg, 0.9 mmol) in DCM (10 mL) at 0° C. was added 1,1,1,3,3,3-hexafluoropropan-2-one (0.14 mL, 0.99 mmol), followed by 30% hydrogen peroxide aqueous solution (0.37 mL, 4.5 mmol) and disodium phosphate (383.3 mg, 2.7 mmol). The reaction mixture was stirred at 0° C. for 10 min and rt overnight. Additional aliquots of reagents (380 mg of disodium phosphate, 0.13 mL of CF₃COCF₃, 0.35 mL of 30% H₂O₂) were added at 24 h and 48 h time points. The reaction was quenched with 10% Na₂S₂O₃solution and extracted with EtOAc. After washing with brine, the organic layer was separated, dried with MgSO₄and concentrated. The residue was purified by silica gel chromatography to provide 470 mg (88%) of desired epoxide as a white foamy solid.

Step E. tert-Butyl (4-((5′R,8′S,9′R,10′R,13′R,14′S)-5′-hydroxy-10′,13′-dimethyl-1′,4′,5′,6′,7′,8′,9′,10′,13′,14′,15′,16′-dodecahydro-2′H-dispiro[[1,3]dioxolane-2,3′-cyclopenta[a]phenanthrene-17′,2″-[1,3]dioxolan]-11′-yl)phenyl)(methyl)carbamate

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A flask was charged with (tert-butyl (4-((4a′R,5a′S,6a′S,6b′S,9a′R,11a′R,11b′R)-9a′,11b′-dimethyl-1′,5a′,6′,6a′,6b′,7′,8′,9a′,11a′,11b′-decahydro-2′H,4′H-dispiro[[1,3]dioxolane-2,3′-cyclopenta[1,2]phenanthro[8a,9-b]oxirene-9′,2″-[1,3]dioxolan]-11′-yl)phenyl)(methyl)carbamate (Step D) (2.98 g, 6.02 mmol) and azeotroped with toluene. Under argon, THF (30 mL) was added and the solution was cooled in an ice-bath followed by dropwise addition of lithium aluminum hydride (1 M solution in THF, 6.02 mL). After 5 min, the ice bath was removed and the reaction was stirred at rt for 1 h. The reaction was quenched with a few drops of methanol, followed by addition of sat. Rochelle's salt solution and EtOAc. The mixture was stirred for 15 min and the organic layer was separated, washed with brine, dried with MgSO4 and concentrated. The residue was purified by silica gel chromatography using a gradient of 0-50% EtOAc in hexanes to provide the title compound (2.63 g, 88%) as a white solid.

STEP F: tert-Butyl (4-((3a′S,3b′S,5a′R,9a′R,9b′S,9c′R,10a′R,10b′R)-5a′-hydroxy-9a′,10b′-dimethyltetradecahydro-9c′H-dispiro[[1,3]dioxolane-2,1′-cyclopenta[1,2]phenanthro[3,4-b]oxirene-7′,2″-[1,3]dioxolane]-9c′-yl)phenyl)(methyl)carbamate

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3-Chlorobenzenecarboperoxoic acid (75%, 3.57 g, 15.5 mmol) was added to a solution of tert-butyl (4-((5′R,8′S,9′R,10′R,13′R,14′S)-5′-hydroxy-10′,13′-dimethyl-1′,4′,5′,6′,7′,8′,9′,10′,13′,14′,15′,16′-dodecahydro-2′H-dispiro[[1,3]dioxolane-2,3′-cyclopenta[a]phenanthrene-17′,2″-[1,3]dioxolan]-11′-yl)phenyl)(methyl)carbamate (Step E) (1.93 g, 3.88 mmol) in DCM (60mL). The reaction was stirred at rt for 20 h and treated with sat. NaHCO₃, and extracted with EtOAc. The organic layer was separated, washed with brine, dried over MgSO₄and concentrated in vacuo. The residue was purified by silica gel chromatography using a gradient of 0-45% EtOAc in hexanes to provide the more polar isomer (530 mg, 27%) as the desired product. The less polar isomer was also isolated (910 mg, 46%).

STEP G: (5′R,8′S,9′R,10′R,11′S,12′S,13′R,14′S)-10′,13′-Dimethyl-11′-(4-(methylamino)phenyl)dodecahydro-2′H-dispiro[[1,3]dioxolane-2,3′-cyclopenta[a]phenanthrene-17′,2″-[1,3]dioxolane]-5′,12′(4′H)-diol

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An oven-dried 3-necked 250 mL flask was fitted with a cold finger condenser and an argon balloon. Both the 3-necked flask and the cold finger were cooled with dry ice in acetone. Liquid ammonia from a supply tank was condensed into the flask until the desired volume of 25 mL was reached. Lithium metal (109 mg, 13.7 mmol) was added and the solution changed to a dark blue color. The dry ice bath was removed briefly for 2 min to speed up the dissolving process of lithium, then the flask was returned to the cooling bath. After about 4 min, a solution of tert-butyl (4-((3a′S,3b′S,5a′R,9a′R,9b′S,9c′R,10a′R,10b′R)-5a′-hydroxy-9a′,10b′-dimethyltetradecahydro-9c′H-dispiro[[1,3]dioxolane-2,1′-cyclopenta[1,2]phenanthro[3,4-b]oxirene-7′,2″-[1,3]dioxolane]-9c′-yl)phenyl)(methyl)carbamate (1.05 g, 1.72 mmol) (Step F) in THF (20 ml) was added dropwise over about 5 min. The reaction was continued for 50 min and its color remained dark blue. At this point, 0.5 mL of ethanol was added, stirred for 1 min, then the cooling bath was removed and water was added slowly to quench the reaction. EtOAc was added and ammonia was allowed to evaporate. The reaction mixture was extracted with EtOAc and washed with brine. The organic layer was dried, concentrated and the residue was purified by silica gel chromatography to give 715 mg of desired product (81%). m/z (ESI, +ve ion) 514.4 (M+H)⁺.

STEP H. (5′R,8′S,9′R,10′R,11′S,12′S,13′R,14′S)-11′-(4-(Dimethylamino)phenyl)-10′,13′-dimethyldodecahydro-2′H-dispiro[[1,3]dioxolane-2,3′-cyclopenta[a]phenanthrene-17′,2″-[1,3]dioxolane]-5′,12′(4′H)-diol

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A flask was charged with (5′R,8′S,9′R,10′R,11′S,12′S,13′R,14′S)-10′,13′-dimethyl-11′-(4-(methylamino)phenyl)dodecahydro-2′H-dispiro[[1,3]dioxolane-2,3′-cyclopenta[a]phenanthrene-17′,2″-[1,3]dioxolane]-5′,12′(4′H)-diol (609 mg, 1.19 mmol) (Step G) and DCM (12 mL) was added, followed by acetic acid (0.68 mL, 11.9 mmol). Then formaldehyde (0.45 mL, 5.93 mmol) was added and the mixture was stirred for 6 min. Sodium triacetoxyborohydride (276 mg, 1.30 mmol) was added. The reaction was stirred for 1 h and quenched with saturated NaHCO₃and extracted with EtOAc. The organic layer was dried, concentrated and purified by silica gel chromatography to give 575 mg of the title compound (92%). m/z (ESI, +ve ion) 528.3 (M+H)⁺.

STEP I. (8S,9R,10R,11S,12S,13R,14S)-11-(4-(Dimethylamino)phenyl)-12-hydroxy-10,13-dimethyl-1,6,7,8,9,10,11,12,13,14,15,16-dodecahydro-3H-cyclopenta[a]phenanthrene-3,17(2H)-dione

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A flask was charged with (5′R,8′S,9′R,10′R,11′S,12′S,13′R,14′S)-11′-(4-(dimethylamino)phenyl)-10′,13′-dimethy ldodecahy dro-2′H-dispiro[[1,3]dioxolane-2,3′-cyclopenta[a]phenanthrene-17′,2″-[1,3]dioxolane]-5′,12′(4′H)-diol (Step H) (940 mg, 1.78 mmol) and acetone (26 mL) was added, followed by 4 N hydrogen chloride (1.78 mL, 7.13 mmol). The resulting clear mixture was stirred at rt for 2 h. The reaction was quenched with sat. NaHCO₃and extracted with EtOAc. The EtOAc layer was washed with brine, dried over MgSO₄and concentrated in vacuo. The residue was purified by silica gel chromatography to give 411 mg (55%) of desired product. m/z (ESI, +ve ion) 422.3 (M+H)⁺.

STEP J. O-((8S,9R,10R,12S,13R,14S)-11-(4-(Dimethylamino)phenyl)-10,13-dimethyl-3,17-dioxo-2,3,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-12-yl) 1H-imidazole-1-carbothioate

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A flask was charged with (8S,9R,10R,11S,12S,13R,14S)-11-(4-(dimethylamino)phenyl)-12-hydroxy-10,13-dimethyl-1,6,7,8,9,10,11,12,13,14,15,16-dodecahydro-3H-cyclopenta[a]phenanthrene-3,17(2H)-dione (Step I) (411 mg, 0.97 mmol) and azeotroped with toluene. DCM (24 mL) was added, followed by triethylamine (0.27 mL, 1.95 mmol) and di(imidazol-1-yl)methanethione (2.8 g, 15.6 mmol). After the reaction was stirred under argon at rt for 4 days, it was quenched with diluted HCl and the crude was extracted with EtOAc. The organic layer was washed with brine twice, dried, and concentrated. The residue was purified by silica gel chromatography to give the title compound (427 mg, 82%).

STEP K. (8S,9R,10R,11S,13S,14S)-11-(4-(Dimethylamino)phenyl)-10,13-dimethyl-1,6,7,8,9,10,11,12,13,14,15,16-dodecahydro-3H-cyclopenta[a]phenanthrene-3,17(2H)-dione

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A flask was charged O-((8S,9R,10R,12S,13R,14S)-11-(4-(dimethylamino)phenyl)-10,13-dimethyl-3,17-dioxo-2,3,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-12-yl) 1H-imidazole-1-carbothioate (Step J) (420 mg, 0.79 mmol) and flushed with argon. Toluene (19mL) was added, followed by tributyltin hydride (0.42mL, 1.58 mmol). After the reaction was refluxed for 3 h, it was cooled to rt and concentrated. The residue was purified by silica gel chromatography to give the title compound (168 mg, 84%) as a colorless oil. m/z (ESI, +ve ion) 406.4 (M+H)⁺.

STEP L. (8S,9R,10R,11S,13S,14S)-11-(4-(Dimethylamino)phenyl)-3-ethoxy-10,13-dimethyl-1,2,7,8,9,10,11,12,13,14,15,16-dodecahydro-17H-cyclopenta[a]phenanthren-17-one

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A flask was charged with (8S,9R,10R,11S,13S,14S)-11-(4-(dimethylamino)phenyl)-10,13-dimethyl-1,6,7,8,9,10,11,12,13,14,15,16-dodecahydro-3H-cyclopenta[a]phenanthrene-3,17(2H)-dione (Step K) (210.mg, 0.52 mmol) and azeotroped with toluene. p-Toluenesulfonic acid monohydrate (118.2 mg, 0.62 mmol) was added to the flask and the flask was flushed with Argon. Ethanol (8 mL) was added, and the reaction was cooled in an ice-bath. Triethyl orthoformate (0.26 mL, 1.55 mmol) was added. The reaction was stirred in the ice-bath for 1 h. Triethylamine (0.72 mL) was added to neutralize the acid. The reaction was concentrated and then directly purified by silica gel chromatography to give the desired mono-ketone (66 mg, 29%). The remaining over-protected and alternate mono-protected products were recycled through hydrolysis with HCl as in Step I. m/z (ESI, +ve ion) 434.4 (M+H)

STEP M. (8S,9R,10R,11S,13S,14S,17S)-11-(4-(Dimethylamino)phenyl)-17-hydroxy-17-(3-methoxyprop-1-yn-1-yl)-10,13-dimethyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one

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A flask was charged with 3-methoxyprop-1-yne (0.1 mL, 1.2 mmol) and THF (2 mL). The reaction mixture was cooled at −78° C. and n-butyllithium (0.71 mL, 1.14 mmol) was added dropwise. The resulting mixture was stirred at −78° C. for 10 min and then added to a solution of (8S,10R,11S,13S,14S)-11-[4-(dimethylamino)phenyl]-3-ethoxy-10,13-dimethyl-1,2,7,8,9,11,12,14,15,16-decahydrocyclopenta[a]phenanthren-17-one (Step L) (29 mg, 0.07 mmol) in THF (2 mL) at −78° C. After the reaction was stirred at −78° C. for 60 min, it was quenched with saturated NH₄Cl and extracted with EtOAc. The organic layer was dried, filtered and concentrated. The residue was dissolved in THF (1.5 mL), 1.2 mL water (1.2 mL) and 4N HCl (0.75 mL) and the solution was stirred at rt for 1 h. The solution was quenched with saturated NaHCO₃solution and extracted with EtOAc. The organic layer was concentrated and the residue was purified by the preparative reverse phase HPLC to give the title compound as the TFA salt (23 mg, 72%). ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 7.05-7.25 (2H, m), 6.48-6.75 (2H, m), 5.68 (1 H, d, J=1.17 Hz), 4.09-4.22 (2H, m), 3.41-3.45 (1H, m), 3.40 (3H, s), 2.94 (6H, s), 2.45 (1H, m), 2.20-2.35 (4H, m), 2.10-2.15 (1H, m), 1.85-2.00 (3H, m), (3H, br d, J=4.53 Hz), 1.68-1.85 (2H, m), 1.36-1.53 (4H, m), 1.02 (3H, s), 0.90 (3H, s); m/z (ESI, +ve ion)=476.4 [M+H]⁺.

Example 2
Compound 9. (8R,9S,10R,11S,13S,14S,17S)-11-(4-(Diethylamino)phenyl)-17-(3,3-dimethylbut-1-yn-1-yl)-17-hydroxy-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one

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STEP A. (8S,13S,14S)-13-Methyl-1,2,4,6,7,8,12,13,14,15,16,17-dodecahydrospiro[cyclopenta[a]phenanthrene-3,2′-[1,3]dioxolan]-17-ol

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To a solution of (8S,13S,14S)-13-methyl-1,4,6,7,8,12,13,14,15,16-decahydrospiro[cyclopenta[a]phenanthrene-3,2′-[1,3]dioxolan]-17(2H)-one (100 g, 318 mmol) in THF (500 mL) and MeOH (50 mL) was added sodium borohydride (6.14 g, 159 mmol) in portions at 0° C. The mixture was stirred at 0° C. for 30 min and then rt for 1 h. After the TLC showed that the starting material disappeared, the reaction was quenched with 2 mL of sat. NaHCO₃solution and concentrated to remove MeOH. The residue was dissolved in EtOAc, the organic phase was washed with sat. NaHCO₃solution, brine, dried over anhydrous Na₂SO₄, and concentrated under reduced pressure to give the title compound (99.5 g, 100% yield) as a colorless oil. The crude material was used for next reaction without further purification. m/z (ESI, +ve ion)=317.3 [M+H]⁺.

STEP B. (5′R,8′S,10′R,13′S,14′S)-13′-Methyl-1′,2′,7′,8′,12′,13′,14′,15′,16′,17′-decahydro-4′H,6′H-spiro[[1,3]dioxolane-2,3′-[5,10]epoxycyclopenta[a]phenanthren]-17′-ol

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To a solution of (8S,13S,14S)-13-methyl-1,2,4,6,7,8,12,13,14,15,16,17-dodecahydrospiro[cyclopenta[a]phenanthrene-3,2′-[1,3]dioxolan]-17-ol (43.0 g, 136 mmol, Step A) in DCM (320 mL) at 0° C. was added Na₂HPO₄(9.65 g, 68.0 mmol), followed by 1,1,1,3,3,3-hexafluoropropan-2-one trihydrate (11.2 mL, 81.5 mmol) and H₂O₂(35% aqueous solution, 34.2 mL, 408 mmol). The mixture was stirred at 0° C. for 10 min and then at rt for 3 h. 10% Sodium thiosulfate solution (200 mL) was slowly added at 0° C. and the mixture was stirred at rt for 30 min and extracted with DCM (300 mL). The organic phase was washed with brine, dried over anhydrous Na₂SO₄, and concentrated to provide the title compound (43.0 g) as a 4:1 mixture of epoxides as a white solid. The crude material was used for next step without further purification.

STEP C. (8S,11R,13S,14S)-11-(4-(Diethylamino)phenyl)-17-hydroxy-13-methyl-1,2,6,7,8,11,12,13,14,15,16,17-dodecahydro-3H-cyclopenta[a]phenanthren-3-one

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A dried flask was charged with magnesium chips (2.04 g, 83 9 mmol) and a crystal of iodine. The flask was flushed with Ar and heated briefly with a heat gun and then put into a 60° C. of oil bath. A small portion of a solution of 4-bromo-N,N-diethylaniline (18.5 g, 81.2 mmol, azeotroped with toluene beforehand) in THF (80 mL) was added (about 5 mL). After the iodine color disappeared, the remaining solution was added dropwise for 20 min and the mixture was continued to stir at 60° C. for 2 h.

The Grignard reagent prepared above was cooled down to rt and added to a solution of (5′R,8′S,10′R,13′S,14′S)-13′-methyl-1′,2′,7′,8′,12′,13′,14′,15′,16′,17′-decahydro-4′H,6′H-spiro[[1,3]dioxolane-2,3′-[5,10]epoxycyclopenta[a]phenanthren]-17′-ol (9.00 g, 27.1 mmol, azeotroped with toluene, Step B) and copper(I) iodide (2.60 g, 13.5 mmol) in THF (100 mL) at 0° C. The resulting mixture was allowed to warm up to rt and stir for 1 h. The reaction was quenched (sat. aq. NH₄Cl), extracted (2×EtOAc), and washed (brine). The combined organic layer was dried (Na₂SO₄) and concentrated under reduced pressure. Purification of the residue by combi-flash (220 g SiO₂, 20% to 80% EtOAc/hexanes) provided (5R,8S,11R,13S,14S)-11-(4-(diethylamino)phenyl)-13-methyl-1,2,6,7,8,11,12,13,14,15,16,17-dodecahydrospiro[cyclopenta[a]phenanthrene-3,2′-[1,3]dioxolane]-5,17(4H)-diol (6.50 g, 13.5 mmol, 50% yield) as an off-white foam.

A solution of (5R,8S,11R,13S,14S)-11-(4-(diethylamino)phenyl)-13-methyl-1,2,6,7,8,11,12,13,14,15,16,17-dodecahydrospiro[cyclopenta[a]phenanthrene-3,2′-[1,3]dioxolane]-5,17(4H)-diol (6.50 g, 13 5 mmol) in 70% Acetic acid in water (109 mL) was stirred at 55° C. for 1 h. The reaction was concentrated under reduced pressure to remove most of AcOH. Then the residue was diluted (EtOAc), basified (sat. aq. NaHCO₃), extracted (2×EtOAc), and washed (brine). The combined organic layer was dried (Na₂SO₄), and concentrated under reduced pressure. Purification of the residue by combi-flash (220 g SiO₂, 20% to 60% EtOAc/hexanes) gave the title compound (6.80 g, 16.2 mmol, 80% yield) as a white solid. ¹H NMR (400 MHz, CHLOROFORM-d) δ 6.98 (d, J=8.33 Hz, 2H), 6.54-6.66 (m, 2H), 5.75 (s, 1H), 4.28 (d, J=6.72 Hz, 1H), 3.66 (br t, J=8.04 Hz, 1H), 3.25-3.38 (m, 4H), 2.68-2.83 (m, 1H), 2.32-2.61 (m, 7H), 1.98-2.16 (m, 2H), 1.60-1.74 (m, 3H), 1.30-1.49 (m, 3H), 1.14 (t, J=7.09 Hz, 6H), 0.48 (s, 3H).

STEP D. (8S,9R,11S,13S,14S)-11-(4-(Diethylamino)phenyl)-17-hydroxy -13-methyl-1,2,4,6,7,8,9,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one

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Under Ar, lithium (283 g, 40.8 mmol) in pieces was added to liquid ammonia (157 mL) at −78° C. When the color changed to dark blue, a solution (8S,11R,13S,14S)-11-(4-(diethylamino)phenyl)-17-hydroxy-13-methyl-1,2,6,7,8,11,12,13,14,15,16,17-dodecahydro-3H-cyclopenta[a]phenanthren-3-one (4.50 g, 10.7 mmol, Step C) in THF (100 mL) was added over 15 min (kept the internal temperature below −60° C.), followed by addition of a solution of tert-butanol (2.50 mL, 25.7 mmol) in THF (50 mL) over 1 min (dark purple color remained after adding). The reaction was quenched by solid ammonium chloride (12.0 g, 225 mmol). Both dry ice bath and cold finger condenser were removed and most ammonia was allowed to evaporate by stirring the slurry at rt for 1 h. The reaction was diluted (saturated aq. NH₄Cl), and extracted (2×EtOAc). The combined organic layer was washed (saturated aq. NH₄Cl), dried (Na₂SO₄), and concentrated under reduced pressure. Purification of the residue by combi-flash (220 g SiO₂, 20% to 60% EtOAc/hexanes) provided the title compound (2.60 g, 6.17 mmol, 58% yield). m/z (ESI, +ve ion)=422.3 [M+H]⁺.

STEP E. (8R,9S,10R,11S,13S,14S)-11-(4-(Diethylamino)phenyl)-17-hydroxy-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one

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To a solution of (8S,9R,11S,13S,14S)-11-[4-(diethylamino)phenyl]-17-hydroxy-13-methyl-2,4,6,7,8,9,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-3-one (2.60 g, 6.17 mmol, Step D) in toluene (50 mL) was added 4-methylbenzenesulfonic acid hydrate (3.52 g, 18.5 mmol) under Ar and the resulting solution was heated at 80° C. for 1 h. The reaction was quenched with TEA (3 mL), cooled, diluted (EtOAc and sat. aq. NaHCO₃), and extracted (2×EtOAc). The combined organic layer was washed (brine), dried (Na₂SO₄), and concentrated under reduced pressure. Purification of the residue by combi flash (120 g, 40% to 60% EtOAc/hexanes) provided the title compound (1.50 g, 3.56 mmol, 58% yield) as a white foam. m/z (ESI, +ve ion)=422.3 [M+H]⁺.

STEP F. (8R,9S,10R,11S,13S,14S)-11-(4-(Diethylamino)phenyl)-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydrospiro[cyclopenta[a]phenanthrene-3,2′-[1,3]dithiolan]-17-ol

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To a solution of (8R,9S,10R,11S,13S,14S)-11-(4-(diethylamino)phenyl)-17-hydroxy-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one (1.50 g, 3.56 mmol, Step E) and p-toluenesulfonic acid monohydrate (1.35 g, 7.11 mmol) in acetic acid (15 mL) was added ethane-1,2-dithiol (3.00 mL, 35.6 mmol). After the solution was stirred at rt for 1 h under Ar, the reaction was quenched (ice-cold 2.5 N aq. NaOH) and extracted (2×EtOAc). The combined organic layer was washed (brine), dried (MgSO4), and concentrated under reduced pressure. Purification of the residue by combi-flash (120 g SiO₂, 20% to 40% EtOAc/hexanes) provided the title compound (740 mg, 1.49 mmol, 42% yield) as a white solid. ¹H NMR (400 MHz, CHLOROFORM-d) δ 7.21 (d, J=8.62 Hz, 2H), 6.58 (d, J=8.92 Hz, 2H), 5.61 (s, 1H), 3.56 (br t, J=7.97 Hz, 1H), 3.25-3.41 (m, 7H), 3.16-3.25 (m, 2H), 2.42-2.57 (m, 1H), 2.27 (td, J=3.27, 13.63 Hz, 1H), 1.93-2.15 (m, 6H), 1.81-1.92 (m, 2H), 1.50-1.73 (m, 2H), 1.21-1.38 (m, 2H), 1.08-1.18 (m, 7H), 0.79-1.05 (m, 3H), 0.54 (s, 3H); m/z (ESI, +ve ion)=498.4 [M+H]⁺.

STEP G. (8R,9S,10R,11S,13S,14S)-11-(4-(Diethylamino)phenyl)-13-methyl-1,6,7,8,9,10,11,12,13,14,15,16-dodecahydrospiro[cyclopenta[a]phenanthrene-3,2′-[1,3]dithiolan]-17(2H)-one

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To a solution of (8R,9 S,10R,11S,13S,14S)-11-(4-(diethylamino)phenyl)-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydrospiro[cyclopenta[a]phenanthrene-3,2′-[1,3]dithiolan]-17-ol (740 mg, 1.49 mmol, Step F) in toluene (15 mL) was added cyclohexanone (0.44 mL, 4.28 mmol) and aluminum propan-2-olate (410 mg, 2.00 mmol) successively. After the reaction was heated to 105° C. for 4 h, it was cooled to rt and quenched with saturated aqueous Rochelle's salt solution. The mixture was stirred for 10 min and extracted (2 x EtOAc). The combined organic layer was washed (brine), dried (MgSO₄), and concentrated under reduced pressure. Purification of the residue by combi-flash (40 g SiO₂, 10% to 50% EtOAc/hexanes) provided the title compound (430 mg, 0.87 mmol, 58% yield) as a white solid. ¹H NMR (400 MHz, CHLOROFORM-d) δ 7.20 (d, J=7.63 Hz, 2H), 6.57 (d, J=8.92 Hz, 2H), 5.60-5.70 (s, 1H), 3.24-3.41 (m, 8H), 3.16-3.24 (m, 1H), 2.37-2.58 (m, 2H), 2.33 (m, 1H), 1.93-2.20 (m, 6H), 1.83 (dt, J=2.78, 13.08 Hz, 1H), 1.66-1.76 (m, 2H), 1.50-1.66 (m, 2H), 1.25-1.37 (m, 3H), 1.02-1.21 (m, 7H), 0.67 (s, 3H); m/z (ESI, +ve ion)=496.4 [M+H]⁺.

STEP H. (8R,9S,10R,11S,13S,14S,17S)-11-(4-(Diethylamino)phenyl)-17-(3,3-dimethylbut-1-yn-1-yl)-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydrospiro[cyclopenta[a]phenanthrene-3,2′-[1,3]dithiolan]-17-ol

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To a solution of isopropylmagnesium chloride (2 M solution in THF, 0.76 mL, 1.51 mmol) in THF (1 mL) was added 3,3-dimethylbut-1-yne (0.19 mL, 0.61 mmol) at 0° C. dropwise under Ar. After the solution was stirred at rt for 10 min, the mixture was stirred at 50° C. for 1 h (with a condenser). The Grignard reagent was cooled to 0° C. and a solution of (8R,9S,10R,11S,13S,14S)-11-[4-(diethylamino)phenyl]-13-methyl-spiro[1,2,6,7,8,9,10,11,12,14,15,16-dodecahydrocyclopenta[a]phenanthrene-3,2′-1,3-dithiolane]-17-one (100 mg, 0.20 mmol, Step G) in THF (2 mL) was added dropwise. After the reaction was stirred at 0° C. for 30 min, it was allowed to warm to rt and stirred overnight. The reaction was quenched (sat. aq. NH₄Cl) and extracted (2×EtOAc). The combined organic layers were washed (brine), dried (MgSO₄), and concentrated under reduced pressure. Purification of the residue by combi-flash (12 g SiO₂, 15% to 35% EtOAc/hexanes) provided the title compound (110 mg, 0.19 mmol, 94% yield) as a white solid; m/z (ESI, +ve ion)=578.5 [M+H]⁺.

STEP I. (8R,9S,10R,11S,13S,14S,17S)-11-(4-(Diethylamino)phenyl)-17-(3,3-dimethylbut-1-yn-1-yl)-17-hydroxy-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one

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To a solution of (8R,9S,10R,11S,13S,14S,17S)-11-(4-(diethylamino)phenyl)-17-(3,3-dimethylbut-1-yn-1-yl)-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydrospiro[cyclopenta[a]phenanthrene-3,2′-[1,3]dithiolan]-17-ol (110 mg, 0.19 mmol, Step H) in acetic acid (4 mL) was added glyoxylic acid monohydrate (876 mg, 9.52 mmol) at rt and the mixture was stirred at rt for 10 min. Then 4 N HCl in water (0.86 mL, 3.43 mmol) was added and the resulting solution was stirred at rt for 20 h. The reaction was quenched (sat. aq. NaHCO₃), extracted (2×EtOAc), and washed (brine). The combined organic layers were dried (Na₂SO₄) and concentrated under reduced pressure. Purification of the residue with combi-flash (12 g SiO₂, 20% to 40% EtOAc/hexanes) provided the title compound (33 mg, 0.066 mmol, 38% yield) as a white foam. ¹H NMR (400 MHz, CHLOROFORM-d) δ 7.23 (br d, J=8.33 Hz, 2H), 6.60 (br d, J=8.77 Hz, 2H), 5.86 (br s, 1H), 3.33 (br d, J=6.87 Hz, 5H), 2.86 (br s, 1H), 2.53 (br d, J=15.05 Hz, 1H), 2.38 (m, 1H), 2.01-2.30 (m, 5H), 1.92 (m, 3H), 1.71 (br s, 1H), 1.46-1.64 (m, 5H), 1.21-1.28 (s, 9H), 1.17 (m, 7H), 0.67 (s, 3H); m/z (ESI, +ve ion)=502.4 [M+H]⁺.

Examples 3-10 were prepared by procedures similar to those described in Example 2, substituting 4-bromo-N,N-diethylaniline with the appropriate bromide in Step C and 3,3-dimethylbut-1-yne with the appropriate alkyne in Step H.

Example 3
Compound 1. (8R,9S,10R,11S,13S,14S,17S)-17-(3,3-Dimethylbut-1-yn-1-yl)-17-hydroxy-13-methyl-11-(4-(pyrrolidin-1-yl)phenyl)-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one

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¹H NMR (400 MHz, CHLOROFORM-d) δ 7.24-7.29 (m, 2H), 6.50 (d, J=8.77 Hz, 2H), 5.86 (s, 1H), 3.22-3.41 (m, 4H), 2.86 (m, 1H), 2.53 (m, 1H), 2.37 (m, 2H), 2.21-2.29 (m, 1H), 1.99-2.20 (m, 7H), 1.89-1.96 (m, 2H), 1.61-1.81 (m, 6H), 1.23-1.43 (m, 11H), 1.01-1.21 (m, 1H), 0.68 (s, 3H); m/z (ESI, +ve ion)=500.5 [M+H]⁺.

Example 4
Compound 3. (8R,9S,10R,11S,13S,14S,17S)-11-(2,3-Dihydrobenzo[b][1,4]dioxin-6-yl)-17-(3,3-dimethylbut-1-yn-1-yl)-17-hydroxy-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one

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¹H NMR (400 MHz, CHLOROFORM-d) δ 6.93 (d, J=1.90 Hz, 1H), 6.83-6.91 (m, 1H), 6.78 (d, J=8.33 Hz, 1H), 5.87 (s, 1H), 4.23-4.32 (m, 4H), 3.33 (br t, J=5.85 Hz, 1H), 2.86 (br s, 1H), 2.53 (br d, J=14.18 Hz, 1H), 2.36-2.42 (m, 1H), 2.11-2.29 (m, 4H), 2.02-2.09 (m, 2H), 1.84-1.99 (m, 3H), 1.69-1.81 (m, 1H), 1.44-1.55 (m, 2H), 1.23-1.41 (m, 11H), 1.01-1.15 (m, 1H), 0.67 (s, 3H); m/z (ESI, +ve ion)=489.4 [M+H]⁺.

Example 5
Compound 5. (8R,9S,10R,11S,13S,14S,17S)-11-(Benzo[d][1,3]dioxol-5-yl)-17-(3,3-dimethylbut-1-yn-1-yl)-17-hydroxy-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one

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¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 6.86-6.94 (2H, m), 6.74 (1H, d, J=8.0 Hz), 5.94-6.00 (2H, m), 5.87 (1H, s), 3.35 (1H, br t, J=6.0 Hz), 2.80-2.90 (1H, m), 2.50-2.57 (1H, m), 2.36-2.42 (1H, m), 2.24-2.35 (2H, m), 1.96-2.23 (5 H, m), 1.87-1.95 (3H, m), 1.69-1.80 (1H, m), 1.44-1.55 (2H, m), 1.38 (1H, td, J=12.0, 5.7 Hz), 1.26-1.33 (1H, m), 1.24 (9H, s), 1.11 (1H, br dd, J=12.1, 3.7 Hz), 0.68 (3H, s); m/z (ESI, +ve ion)=475.4 [M+H]⁺.

Example 6
Compound 4. (8R,9S,10R,11S,13S,14S,17S)-17-Hydroxy-11-(4-(isopropyl(methyl)amino)phenyl)-17-(3-methoxy-3-methylbut-1-yn-1-yl)-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one

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¹H NMR (400 MHz, CHLOROFORM-d) δ 7.25 (br d, J=8.6 Hz, 2H), 6.71 (br d, J=8.77 Hz, 2H), 5.86 (s, 1H), 4.08 (td, J=6.56, 13.34 Hz, 1H), 3.37 (s, 3H), 3.33 (br t, J=5.41 Hz, 1H), 2.85 (br m, 1H), 2.73 (s, 3H), 2.53 (br d, J=14.32 Hz, 1H), 2.18-2.43 (m, 4H), 1.89-2.16 (m, 6H), 1.67-1.80 (m, 2H), 1.37-1.55 (m, 8H), 1.26 (dt, J=5.55, 11.40 Hz, 1H), 1.05-1.21 (m, 7H), 0.69 (s, 3H); m/z (ESI, +ve ion)=518.5 [M+H]⁺.

Example 7
Compound 7. (8R,9S,10R,11S,13S,14S,17S)-17-(3,3-Dimethylbut-1-yn-1-yl)-17-hydroxy-13-methyl-11-(4-morpholinophenyl)-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one

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¹H NMR (400 MHz, CHLOROFORM-d) δ 7.33 (br d, J=8.33 Hz, 2H), 6.84 (br d, J=7.45 Hz, 2H), 5.87 (s, 1H), 3.88 (br s, 4H), 3.26-3.48 (m, 1H), 3.17 (br s, 4H), 2.82 (br s, 1H), 2.54 (br d, J=14.03 Hz, 1H), 2.33-2.46 (m, 1H), 2.03-2.31 (m, 5H), 1.82-1.99 (m, 3H), 1.67-1.80 (m, 1H), 1.45-1.65 (m, 3H), 1.21-1.42 (m, 11H), 1.07-1.18 (m, 1H), 0.64 (s, 3H); m/z (ESI, +ve ion)=516.3 [M+H]⁺.

Example 8
Compound 6. (8R,9S,10R,11S,13S,14S,17S)-11-(4-(Dimethylamino)phenyl)-17-(3,3-dimethylpent-1-yn-1-yl)-17-hydroxy-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one

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¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 7.27 (2H, s), 6.67 (2H, br d, J=6.4 Hz), 5.86 (1H, s), 3.34 (1H, br s), 2.95 (3H, br), 2.95 (3H, br), 2.83 (1H, br d, J=10.8 Hz), 2.49-2.57 (1H, m), 2.37 (1H, td, J=14.1, 4.3 Hz), 2.27 (2H, dt, J=16.3, 3.9 Hz), 2.19 (1H, ddd, J=13.8, 9.6, 5.7 Hz), 2.02-2.14 (3H, m), 1.87-1.99 (3H, m), 1.69-1.78 (1 H, m), 1.63 (1H, s), 1.48-1.54 (2H, m), 1.44(2H, q, J=7.6 Hz), 1.33-1.41 (1H, m), 1.22-1.31 (1 H, m), 1.19(3H, s), 1.19 (3H, s), 1.10 (1H, br d, J=12.0 Hz), 0.99 (3H, t, J=7.6 Hz), 0.66 (3H, s); m/z (ESI, +ve ion)=488.5 [M+H]⁺.

Example 9
Compound 11. (8R,9S,10R,11S,13S,14S,17S)-11-(4-(tert-Butyl(methypamino)phenyl)-17-(3,3-dimethylbut-1-yn-1-yl)-17-hydroxy-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one

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¹H NMR (400 MHz, CHLOROFORM-d) δ 7.25-7.30 (m, 2H), 7.01-7.05 (m, 2H), 5.85 (s, 1H), 3.37 (s, 1H), 2.81-2.93 (m, 1H), 2.74 (s, 3H), 2.50 (m, 1H), 2.31-2.41 (m, 1H), 1.98-2.31 (m, 5H), 1.86-1.96 (m, 3H), 1.64-1.74 (m, 1H), 1.43-1.53 (m, 2H), 1.16-1.38 (m, 12H), 1.04-1.14 (m, 10H), 0.56 (s, 3H); MS (ESI)=516.5 [M+H]⁺.

Example 10
Compound 2. (8R,9S,10R,11S,13S,14S,17S)-17-(3,3-Dimethylbut-1-yn-1-yl)-17-hydroxy-11-(4-(isopropyl(methyl)amino)phenyl)-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one

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¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 7.23 (2H, br d, J=8.3 Hz), 6.71 (2H, br d, J=8.3 Hz), 5.86 (1H, s), 4.04-4.11 (1H, m), 3.33 (1H, br s), 2.86 (1H, br s), 2.72 (3H, s), 2.53 (1H, br d, J=14.3 Hz), 2.33-2.43 (1H, m), 2.07-2.31 (5H, m), 2.03 (1H, br s), 1.88-1.97 (3H, m), 1.68-1.78 (1H, m), 1.63 (1H, s), 1.46-1.55 (2H, m), 1.34-1.42 (1H, m), 1.26-1.30 (1H, m), 1.24 (9H, s), 1.17 (3H, d, J=5.6 Hz), 1.15 (3H, d, J=5.6 Hz), 1.06-1.12 (1H, m), 0.67 (3H, s); m/z (ESI, +ve ion)=502.4 [M+H]⁺.

Example 11
Compound 10. (8R,9S,10R,11S,13S,14S,17S)-17-(3,3-Dimethylbut-1-yn-1-yl)-17-hydroxy-11-(4-(isopropylamino)phenyl)-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one

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Step A. (8S,11R,13S,14S)-11-(4-Aminophenyl)-17-hydroxy-13-methyl-1,2,6,7,8,11,12,13,14,15,16,17-dodecahydro-3H-cyclopenta[a]phenanthren-3-one

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The title compound was prepared from (5′R,8′S,10′R,13′S,14′S)-13′-methyl-1′,2′,7′,8′,12′,13′,14′,15′,16′,17′-decahydro-4′H,6′H-spiro[[1,3]dioxolane-2,3′-[5,10]epoxycyclopenta[a]phenanthren]-17′-ol (Example 2, Step B) by a procedure similar to that described in Example 2, Step C, substituting (4-(bis(trimethylsilyl)amino)phenyl)magnesium bromide for (4-(diethylamino)phenyl)magnesium bromide.

Step B. tert-Butyl (4-((8S,11R,13S,14S)-17-hydroxy-13 -methyl-3-oxo-2,3,6,7,8,11,12,13,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-11-yl)phenyl)carbamate

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To a flask charged with (8S,11R,13S,14S)-11-(4-aminophenyl)-17-hydroxy-13-methyl-1,2,6,7,8,11,12,13,14,15,16,17-dodecahydro-3H-cyclopenta[a]phenanthren-3-one (4.00 g, 11.0 mmol, azeotroped with toluene, Step A) were added di-tert-butyl dicarbonate (12.0 g, 55.0 mmol) and MeCN (72 mL). The reaction was stirred overnight. Then the solid was filtered and rinsed (v/v=5/1, hep/EtOAc). The filtrate was purified by combi-flash (80 g SiO₂, 25% to 50% EtOAc/hexanes) to provide the title compound (4.90 g, 10.6 mmol, 96% yield) as a yellow solid.

Step C. (8R,9S,10R,11S,13S,14S)-11-(4-Aminophenyl)-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydrospiro[cyclopenta[a]phenanthrene-3,2′-[1,3]dithiolan]-17-ol

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The title compound was prepared from tert-butyl (4-((8S,11R,13S,14S)-17-hydroxy-13-methyl-3-oxo-2,3,6,7,8,11,12,13,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-11-yl)phenyl)carbamate (Step B) by procedures similar to those described in Example 2, Steps D, E, and F.

Step D. (8R,9S,10R,11S,13S,14S)-11-(4-(Isopropylamino)phenyl)-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydrospiro[cyclopenta[a]phenanthrene-3,2′-[1,3]dithiolan]-17-ol

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To a solution of acetone (86.4 uL, 1.18 mmol) and (8′R,11′S,13′S,14′S)-11′-(4-aminophenyl)-13′-methyl-spiro[1,3-dithiolane-2,3′-2,6,7,8,9,10,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthrene]-17′-ol (19.2 mL, 0.12 mmol, Step C) in DCE (1.1 mL) was added sodium triacetoxyborohydride (62.4 mg, 0.29 mmol) at rt. After the reaction was stirred at rt, it was basified (sat. aq. NaHCO₃), extracted (2×DCM), and washed (water). The combined organic layer was dried (Na₂SO₄) and concentrated under reduced pressure. Purification of the residue by combi-flash (12 g SiO₂, 30% to 50% EtOAc/hexanes) provided the title compound (51.0 mg, 0.105 mmol, 90% yield) as an off white solid. m/z (ESI, +ve ion)=486.4 [M+H]⁺.

Step E. (8R,9S,10R,11S,13S,14S,17S)-17-(3,3-Dimethylbut-1-yn-1-yl)-17-hydroxy-11-(4-(isopropylamino)phenyl)-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one

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The title compound was prepared from (8R,9S,10R,11S,13S,14S)-11-(4-(isopropylamino)phenyl)-13 -methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydrospiro[cyclopenta[a]phenanthrene-3,2′-[1,3]dithiolan]-17-ol (Step D) by procedures similar to those described in Example 2, Steps G, H, and I.

¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 7.19 (d, J=7.89 Hz, 2H), 6.52 (d, J=7.75 Hz, 2H), 5.85 (s, 1H), 3.57-3.64 (m, 1H), 3.28-3.32 (m, 1H), 2.79-2.86 (m, 1H), 2.49-2.55 (m, 1H), 2.30-2.40 (m, 1H), 2.01-2.29 (m, 6H), 1.86-1.97 (m, 3H), 1.69-1.74 (m, 1H), 1.63 (s, 1H), 1.37-1.55 (m, 3H), 1.30-1.40 (m, 1H), 1.20-1.28 (m, 15H), 0.66 (s, 3H); m/z (ESI, +ve ion)=488.4 [M+H]⁺.

Example 12
Compound 13. (8R,9S,10R,11S,13S,14S,17S)-11-(4-Aminophenyl)-17-(3,3-dimethylbut-1-yn-1-yl)-17-hydroxy-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one

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Step A. tert-Butyl (4-((8R,9S,10R,11S,13S,14S)-17-hydroxy-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydrospiro[cyclopenta[a]phenanthrene-3,2′-[1,3]dithiolan]-11-yl)phenyl)carbamate

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A flask was charged with (8R,9S,10R,11S,13S,14S)-11-(4-Aminophenyl)-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydrospiro[cyclopenta[a]phenanthrene-3,2′-[1,3]dithiolan]-17-ol (358 mg, 0.81 mmol, azeotroped with toluene, Example 11, Step C), di-tert-butyl dicarbonate (885 mg, 4.05 mmol) and MeCN (6mL) was added. After the reaction was stirred overnight, the solid was filtered and rinsed with 5:1 heptane:EtOAc. The filtrate was purified by combi-flash (40 g SiO₂, 25% to 50% EtOAc/hexanes) to provide the title compound (420 mg, 0.780 mmol, 95% yield).

Step B. tert-Butyl (4-((8R,9S,10R,11S,13S,14S,17S)-17-(3,3 -dimethylbut-1-yn-1-yl)-17-hydroxy-13-methyl-3-oxo-2,3,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-11-yl)phenyl)carbamate

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The title compound was prepared from tert-butyl (4-((8R,9S,10R,11S,13S,14S)-17-hydroxy-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydrospiro[cyclopenta[a]phenanthrene-3,2′-[1,3]dithiolan]-11-yl)phenyl)carbamate (Step A) by procedures similar to those described in Example 2, Steps G, H, and I.

Step C. (8R,9 S,10R,11S,13S,14S,17S)-11-(4-Aminophenyl)-17-(3,3-dimethylbut-1-yn-1-yl)-17-hydroxy-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one

A flask was charged with tert-butyl (4-((8R,9S,10R,11S,13S,14S,17S)-17-(3,3-dimethylbut-1-yn-1-yl)-17-hydroxy-13-methyl-3-oxo-2,3,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-11-yl)phenyl)carbamate (39 mg, 0.07 mmol, Step B) in acetone (1 mL), followed by addition of 4 N HCl in dioxane (0.09 mL, 0.36 mmol). After the resulting clear mixture was stirred at rt for 5 h, it was quenched with sat NaHCO₃solution and then extracted with EtOAc. The organic layer was washed with brine, dried over anhydrous sodium sulfate, and concentrated. The crude product was purified by combi-flash (4 g SiO₂, 0 to 70% EtOAc/hexanes) to provide the title compound (8.0 mg, 0.018 mmol, 25%) as an off-white solid. ¹H NMR (400 MHz, DICHLOROMETHANE-d2) δ ppm 7.12 (d, J=8.33 Hz, 2H), 6.49-6.55 (d, 2H), 5.71 (s, 1H), 3.22 (br t, J=5.92 Hz, 1H), 2.71-2.77 (m, 1H), 2.40-2.45 (m, 1H), 2.23-2.33 (m, 1H), 1.91-2.19 (m, 6H), 1.70-1.85 (m, 3H), 1.54-1.69 (m, 2H), 1.34-1.48 (m, 3H), 1.22-1.34 (m, 1H), 1.14 (s, 9H), 0.54 (s, 3H); m/z (ESI, +ve ion)=446.3 [M+H]⁺.

Example 13
Compounds 14 and 15. (8R,9S,10R,11S,13S,14S,17S)-17-(3,3-dimethylbut-1-yn-1-yl)-11-(3-fluoro-4-(isopropylamino)phenyl)-17-hydroxy-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one (14) and (8R,9S,10R,11S,13S,14S,17S)-17-(3,3-Dimethylbut-1-yn-1-yl)-11-(3-fluoro-4-(isopropyl(methyl)amino)phenyl)-17-hydroxy-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one (15)

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A dry flask was charged with (8R,9S,11S,13S,14S,17S)-17-(3,3-dimethylbut-1-ynyl)-17-hydroxy-11-[4-[isopropyl(methyl)amino]phenyl]-13-methyl-1,2,6,7,8,9,10,11,12,14,15,16-dodecahydrocyclopenta[a]phenanthren-3-one (300 mg, 0.60 mmol, Example 10) and DCM (8 mL). The flask was cooled to 0° C. and 1-fluoro-2,4,6-trimethylpyridinium trifluoromethanesulfonate (294 mg, 1.02 mmol) was added. After the reaction was stirred at 0° C. for 60 min, it was warmed up to rt and stirred for another 30 min. The reaction mixture was quenched with NaHCO₃solution (6 ml) and extracted with EtOAc (200 ml). The organic layer was washed with brine, dried over anhydrous sodium sulfate, and concentrated. The residue was purified by combi-flash eluting with 1:9 EtOAc/hexanes to provide a mixture of 14 and 15. The mixture was purified by preparative reverse-phase HPLC (gradient elution of 10% to 90% MeCN in water, which both solvents contain 0.1% formic acid, 30 min method) to give (8R,9S,10R,11S,13S,14S,17S)-17-(3,3-dimethylbut-1-yn-1-yl)-11-(3-fluoro-4-(isopropyl(methyl)amino)phenyl)-17-hydroxy-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one (15) as the first eluting isomer and (8R,9S,10R,11S,13S,14S,17S)-17-(3,3 -dimethylbut-1-yn-1-yl)-11-(3-fluoro-4-(isopropylamino)phenyl)-17-hydroxy-13-methyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one (14) as the second eluting isomer.

Compound 14: ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 6.99-7.22 (m, 2H), 6.62 (t, J=8.62 Hz, 1H), 5.87 (s, 1H), 3.47-3.75 (m, 1H), 3.22-3.46 (m, 1H), 2.76-2.86 (br dd, J=3.00, 1.68 Hz, 1H), 2.530-2.58 (br d, J=14.32 Hz, 1H), 2.34-2.43 (m, 1H), 2.21-2.35 (m, 2H), 2.14-2.211 (m, 1H), 2.05-2,14 (m, 1H), 2.02-2.05 (m, 1H), 1.83-2.00 (m, 2H), 1.68-1.78 (m, 1H), 1.64-1.68 (m, 1H), 1.45-1.55 (m, 3H), 1.32-1.42 (m, 1H), 1.24-1.27 (d, J=4 Hz, 3H), 1.20-1.24 (m, 12H), 1.05-1.15 (m, 2H), 0.57-0.68 (m, 3H); m/z (ESI, +ve ion) 506.5 (M+H)⁺.

Compound 15: ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 6.98-7.17 (m, 2H), 6.84 (t, J=8.84 Hz, 1H), 5.87 (s, 1H), 3.66-3.81 (m, 1H), 3.35 (br t, J=5.12 Hz, 1H,), 2.84 (br dd, J=3.29, 1.53 Hz, 1H), 2.69 (s, 3H), 2.50-2.59 (m, 1H), 2.38-2.43 (m, 1H), 2.22-2.36 (m, 2H), (dt, J=1.97, 1.06 Hz, 1H), 2.10-2.22 (m, 2H), 2.01-2.12 (m, 2H), 1.84-2.00 (m, 2H), 1.69-1.81 (m, 1H), 1.66 (s, 1H), 1.43-1.56 (m, 2H), 1.34-1.44 (m, 1H), 1.25-1.33 (m, 3H), 1.24 (9 s, H), 1.07-1.18 (m, 6H), 0.63 (s, 3H); m/z (ESI, +ve ion) 520.4 (M+H)⁺.

Example 14
Compound 8. (8R,9S,10R,11S,13S,14S,16R,17S)-17-(3,3-Dimethylbut-1-yn-1-yl)-17-hydroxy-11-(4-(isopropyl(methyl)amino)phenyl)-13,16-dimethyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one or (8R,9S,10R,11S,13S,14S,16S,17S)-17-(3,3-dimethylbut-1-yn-1-yl)-17-hydroxy-11-(4-(isopropyl(methyl)amino)phenyl)-13,16-dimethyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one

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Step A. (8R,9S,10R,11S,13S,14S,16R)-11-(4-(Isopropyl(methyl)amino)phenyl)-13,16-dimethyl-1,6,7,8,9,10,11,12,13,14,15,16-dodecahydrospiro[cyclopenta[a]phenanthrene-3,2′-[1,3]dithiolan]-17(2H)-one or (8R,9S,10R,11S,13S,14S,16S)-11-(4-(isopropyl(methyl)amino)phenyl)-13,16-dimethyl-1,6,7,8,9,10,11,12,13,14,15,16-dodecahydrospiro[cyclopenta[a]phenanthrene-3,2′-[1,3]dithiolan]-17(2H)-one

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To a solution of (8S,13S,14S)-13-methyl-1,4,6,7,8,12,13,14,15,16-decahydrospiro[cyclopenta[a]phenanthrene-3,2′-[1,3]dioxolan]-17(2H)-one (8.00 g, 25.4 mmol, an intermediate of Example 10 in Step G) and 3 ml of DMPU in THF (50 mL) at −45° C. was added [bis(trimethylsilyl)amino]lithium (50.9 mL, 50.9 mmol). After 30 min stirring, iodomethane (7.92 mL, 127 mmol) was added. The reaction was stirred at −20° C. for 1 h and was quenched with sat.NH₄Cl, extracted with EtOAc, washed with brine, dried over Na₂SO₄, and concentrated under reduced pressure. The residue was purified by a combi-flash (10-20% EtOAc/hexanes) to give the title compound (6.70 g, 20.4 mmol, 80% yield).

Step B. (8R,9S,10R,11S,13S,14S,16R,17S)-17-(3,3-Dimethylbut-1-yn-1-yl)-17-hydroxy-11-(4-(isopropyl(methyl)amino)phenyl)-13,16-dimethyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one or (8R,9S,10R,11S,13S,14S,16S,17S)-17-(3,3-dimethylbut-1-yn-1-yl)-17-hydroxy-11-(4-(isopropyl(methyl)amino)phenyl)-13,16-dimethyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one

The title compound was prepared from (8R,9S,10R,11S,13S,14S,16R)-11-(4-(isopropyl(methyl)amino)phenyl)-13,16-dimethyl-1,6,7,8,9,10,11,12,13,14,15,16-dodecahydrospiro[cyclopenta[a]phenanthrene-3,2′-[1,3]dithiolan]-17(2H)-one or (8R,9S,10R,11S,13S,14S,16S)-11-(4-(isopropyl(methyl)amino)phenyl)-13,16-dimethyl-1,6,7,8,9,10,11,12,13,14,15,16-dodecahydrospiro[cyclopenta[a]phenanthrene-3,2′-[1,3]dithiolan]-17(2H)-one (Step A) by procedures similar to those described in Example 2, Steps H and I.

¹H NMR (400 MHz, CHLOROFORM-d) δ 7.24 (d, J=8.77 Hz, 2H), 6.71 (d, J=8.92 Hz, 2H), 5.85 (s, 1H), 4.02-4.13 (m, 1H), 3.27-3.37 (m, 1H), 2.77-2.92 (m, 1H), 2.67-2.73 (m, 3H), 2.47-2.58 (m, 1H), 2.36 (m, 1H), 1.85-2.31 (m, 8H), 1.57-1.75 (m, 1H), 1.44-1.57 (m, 2H), 1.19-1.30 (m, 11H), 1.16 (dd, J=5.12, 6.58 Hz, 6H), 1.11 (m, 4H), 0.73 (s, 3H); m/z (ESI, +ve ion)=516.5 [M+H]⁺.

Example 15
Compound 21. (8S,9R,10R,11S,13S,14S,17S)-17-(Cyclopropylethynyl)-17-hydroxy-11-(4-(isopropyl(methypamino)phenyl)-10,13-dimethyl-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one

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Example 15 was prepared from (8′S,9′S,10′R,13′R,14′S)-10′,13′-dimethyl-1′,4′,7′,8′,9′,10′,13′,14′,15′,16′-decahydro-2′H-dispiro[[1,3]dioxolane-2,3′-cyclopenta[a]phenanthrene-17′,2″-[1,3]dioxolan]-11′-yl 1,1,2,2,3,3,4,4,4-nonafluorobutane-1-sulfonate (Example 1, Step B) by procedures similar to those described in Example 1, Steps C-M, substituting formaldehyde with acetone in Step H and substituting 3-methoxyprop-1-yne with ethynylcyclopropane in Step M. ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 7.20-7.40 (m, 2H), 6.64 (d, J=8.77 Hz, 2H), 5.68 (d, J=1.32 Hz, 1H), 4.05 (m, 1H), 3.50 (s, 1H), 3.40 (m, 1H), 2.71 (s, 3H), 2.50 (m, 1H), 2.20-2.40 (m, 5H), 2.10-2.20 (m, 2H), 1.95 (m, 3H), 1.73 (m, 2H), 1.43 (m, 3H), 1.30 (m, 2H), 1.16 (dd, J=6.58, 1.17 Hz, 6H), 1.02 (s, 3H), 0.87 (s, 3H), 0.74-0.84 (m, 2H), 0.60-0.73 (m, 2H); m/z (ESI, +ve ion)=486.4 [M+H]⁺.

Example 16
Compound 26. (8S,9R,10R,11S,13S,14S,17S)-11-(4-Aminophenyl)-17-hydroxy-10,13-dimethyl-17-(prop-1-yn-1-yl)-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one

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Example 16 was prepared from (8′S,9′S,10′R,13′R,14′S)-10′,13′-dimethyl-1′,4′,7′,8′,9′,10′,13′,14′,15′,16′-decahydro-2′H-dispiro[[1,3]dioxolane-2,3′-cyclopenta[a]phenanthrene-17′,2″-[1,3]dioxolan]-11′-yl 1,1,2,2,3,3,4,4,4-nonafluorobutane-1-sulfonate (Example 1, Step B) by procedures similar to those described in Example 1, Steps C-G, substituting (4-((tert-butoxycarbonyl)(methyl)amino)phenyl)boronic acid with (4-((tert-butoxycarbonyl)aminophenyl)boronic acid in Step C, and followed by procedures similar to those described in Example 1, Steps I-M, substituting 3-methoxyprop-1-yne with prop-1-yne in Step M. ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 7.26-7.05 (br s, 2H), 6.54-6.62 (m, 2H), 5.69 (d, J=1.32 Hz, 1H), 3.59 (br s, 2H), 3.41 (t, J=5.55 Hz, 1H), 2.46-2.59 (m, 1H), 2.11-2.33 (m, 7H), 1.86-1.99 (m, 3H), 1.89 (s, 3H), 1.64-1.81 (m, 3H), 1.36-1.56 (m, 3H), 1.10-1.23 (m, 1H), 1.03 (s, 3H), 0.87 (s, 3H); m/z (ESI, +ve ion)=418.3 [M+H]⁺.

Example 17
Compound 30. (8S,9R,10R,11S,13S,14S,17S)-17-Hydroxy-11-(4-(isopropylamino)phenyl)-10,13-dimethyl-17-(prop-1-yn-1-yl)-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one

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Example 17 was prepared from 8S,9R,10R,11S,13S,14S,17S)-11-(4-aminophenyl)-17-hydroxy-10,13-dimethyl-17-(prop-1-yn-1-yl)-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one (Example 16) by procedure similar to that described in Example 1, Step H, substituting formaldehyde with acetone. ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 7.25-7.05 (br s, 2H), 6.46 (d, J=8.77 Hz, 2H), 5.68 (d, J=1.17 Hz, 1H), 3.61 (quin, J=6.29 Hz, 1H), 3.33-3.43 (m, 2H), 2.47-2.56 (m, 1H), 2.10-2.34 (m, 7H), 1.86-1.99 (m, 3H), 1.89 (s, 3H), 1.70-1.84 (m, 3H), 1.36-1.51 (m, 3H), 1.22 (d, J=4.0 Hz, 1H), 1.21 (d, J=4.0 Hz, 1H), 1.09-1.18 (m, 1H), 1.03 (s, 3H) 0.88 (s, 3H); m/z (ESI, +ve ion)=460.4 [M+H]⁺.

Example 18
Compound 31. (8S,9R,10R,11S,13S,14S,17S)-11-(4-(1H-Pyrrol-1-yl)phenyl)-17-hydroxy-10,13-dimethyl-17-(prop-1-yn-1-yl)-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one

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Example 18 was prepared from 8S,9R,10R,11S,13S,14S,17S)-11-(4-aminophenyl)-17-hydroxy-10,13-dimethyl-17-(prop-1-yn-1-yl)-1,2,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-3H-cyclopenta[a]phenanthren-3-one (Example 16) by procedure similar to that described in Example 1, Step H, substituting formaldehyde with 2,5-dimethoxytetrahydrofuran. ¹H NMR (400 MHz, CHLOROFORM-d) δ ppm 7.40-7.65 (br s, 2H), 7.23-7.27 (m, 2H), 7.10 (t, J=2.19 Hz, 2H), 6.35 (t, J=2.19 Hz, 2H), 5.71 (d, J=1.02 Hz, 1H), 3.55 (br t, J=5.85 Hz, 1H), 2.54 (td, J=14.47, 4.53 Hz, 1H), 2.15-2.39 (m, 7H), 1.94-2.01 (m, 3H), 1.91 (s, 3H), 1.70-1.85 (m, 3H), 1.41-1.58 (m, 3H), 1.12-1.23 (m, 1H), 1.07 (s, 3H), 0.86 (s, 1H); m/z (ESI, +ve ion) =468.4 [M+H]⁺.

II. Biological Evaluation
Example A
In Vivo GR Luciferase Assay

Cell Line: CHO/GR/MMTV Luciferase

Culture Media: DMEM (with phenol red)+10% FBS

Assay Media: DMEM (without phenol red)+10% CSS

A 1×10 cm plate of cells was split into 2×15 cm plates in Assay Media (CSS). After 4 days, the cells in the Assay Media were collected and counted.

Antagonist Assay: 1.5×10⁴cells per well were plated in a 384-well white walled TC plate in 20 ul of Assay Media containing 12.5 nM Dexamethasone (final concentration=10 nM).

The plates were covered with film to avoid evaporation and placed in a TC incubator. Media/drug dilutions were prepared in Assay Media in 96-well sterile plates.

100 ul of Assay Media was distributed in aliquots into 96-well plates with an 8-Channel pipette (20-200 ul). 0.5 ul of 1000× concentration of compounds were added to the plates with 8-Channel pipette (0.5-10 ul). The plates were shaken for 15 seconds to mix. The film was removed from the 384-well plates with assay cells. 5 ul of media/drug dilutions were added to appropriate wells. The plates were covered with film to avoid evaporation and placed in a TC incubator overnight. After 16-18 h in the incubator, the plates were removed and sat at room temperature for 10 minutes to equilibrate. The Reagent Dispenser was setup to distribute 25 ul aliquots of OneGlo Luciferase Reagent (at room temperature) to each well of the 384-well plate using standard cassette. After OneGlo was added to the plates, the plates were shaken briefly, then incubated in the dark at room temperature for about 3 minutes. The plates were read using a Tecan or Promega luminometer.

The ability of the compounds disclosed herein to inhibit GR activity was quantified and the respective IC₅₀value was determined. Table 3 provides the cellular IC₅₀values of various substituted steroidal compounds disclosed herein.

TABLE 3

No.
GR IC₅₀(nM)

1
A

2
A

3
A

4
A

5
A

6
A

7
A

8
A

9
A

10
A

11
A

12
B

13
A

14
B

15
A

16
B

17
B

18
A

19
B

20
A

21
A

22
A

23
B

24
A

25
A

26
B

27
A

28
B

29
A

30
B

31
A

32
B

Note:

Cellular assay IC₅₀data are designated within the following ranges:

A: ≤100 nM

B: >100 nM

Example B
In Vitro AR Agonism Assay

The AR agonism assay was done in the LNAR reporter cell line which has overexpressed AR and 4XARE-Luc genes. This cell line is sensitive to even minor partial AR agonism activity in hormone-deprived media (CSS). The assays were done in RPMI (without phenol red)+10% CSS using 6000 LNAR cells/well in 384-well plates and compounds were incubated with cells in 370 C incubator for 18-24 hrs. OneGlo reagent (25 uL/well) was added and plates were read with luminometer within 3 minutes.

Mifepristone shows strong partial AR agonism in concentrations as low as 10 nM, and is known to significantly promote CRPC growth both in vivo and in vitro. To gauge the AR partial agonism activity, Mifepristone is included as a standard in the assay. AR agonism assay results are quantified by determining the ratio of the maximum response of individual compounds to the maximum response of Mifepristone (arbitrarily set to 1). Values of Table 4 are reported as Relative Maximum Response to Mifepristone. This general trend of AR agonism was shown for the class of compounds described herein.

TABLE 4

Relative Maximum Response to

No.
Mifepristone

1
A

2
A

4
A

6
A

7
A

8
A

10
A

11
A

14
A

17
A

18
A

19
A

20
A

21
A

22
A

25
A

32
A

Note:

AR agonism response assay data relative maximum response to Mifepristone is designated within the following ranges:

A: ≤0.4

B: >0.4

	Number	Date	Country
	62405801	Oct 2016	US
	62526331	Jun 2017	US

	Number	Date	Country
Parent	16365422	Mar 2019	US
Child	16562204		US

	Number	Date	Country
Parent	PCT/US2017/055660	Oct 2017	US
Child	16365422		US

INHIBITORS OF GLUCOCORTICOID RECEPTOR

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