Patent Application
20170258992
The present invention relates to a method of controlling an injection pump, and further relates to a related control unit and an injection pump.
Diabetes is a chronic disease that heavily endangers human health in the modern society. In practice, it is most effective to use insulin to control blood glucose and prevent and control various serious complications. Since insulin cannot be taken orally, it is conventional to use an injector to inject insulin. Currently, there are also increasingly more examples of injection using injection pumps.
An injection pump is used to accurately inject insulin at a fixed frequency in a fixed quantity so as to achieve the purposes of better controlling the blood glucose concentration in diabetic patients, reducing the fluctuation of the blood glucose, and reducing the occurrence of hypoglycemia, thereby improving the patients' quality of life. The presence of an injection pump was once considered as good news for diabetic patients. However, popularity of injection pumps is at a low level at present. Apart from factors such as high costs, the trouble caused to the medical workers and patients by tedious process of setting an injection dosage of a pump also restricts the application and promotion of the injection pumps.
Currently, the dosage of an injection pump is set according to a scheme of “total dosage—basic dosage—additional dosage”, i.e., the total amount of insulin is calculated according to the weight and conditions of a patient, or the past usage and the therapeutic effect of the insulin; the total dosage is multiplied by a distribution coefficient to respectively obtain a basic dosage and a before meal (during meal) additional dosage. However, the method cannot enable a patient's blood glucose concentration to rapidly reach an expected range. Usually, four times of monitoring of the blood glucose are needed every day, and the amount of insulin is constantly adjusted according to the blood glucose concentration. The operation of initially setting the dosage of an injection pump is usually accomplished by medical staff In order to find out a comparatively satisfactory amount of insulin, the diabetic patients are often required to stay in hospital for a week or so; if the patients choose not to stay in hospital, then it will take longer time to accomplish the setting of the dosage of the injection pump. Additionally, since the kinds and times of meal for a patient might change every day, it then requires the user of the pump to acquire the calculation method of the before meal/during meal additional amount. In this regard, it sets out relatively higher requirements for users' learning ability. However, many patients abandon pump treatment because of disability to accommodate and acquire the treatment. On the other hand, there are various insulin preparations that can be chosen by clinical doctors during conventional treatment, including rapid-acting, short-acting, intermediate-acting and long-acting insulin. Each preparation has its pharmacokinetics/pharmacodynamics characteristics and indications, but the injection pumps only employ rapid-acting or short-acting insulin. Such scheme of setting differs significantly from daily therapeutic scheme, and the medical staff need to be trained to acquire the setting method. Nevertheless, most medical staff do not understand the scheme of setting such kind of injection pumps, which also becomes a restrictive factor of the application of injection pumps.
With respect to the abovementioned problems, the present invention proposes a solution. According to the present invention, the use of rapid-acting or short-acting preparation in combination with an injection pump to simulate the pharmacokinetics/pharmacodynamics characteristics of long-acting or intermediate-acting preparations extremely facilitates the medical staff and the patients to use the injection pumps, thereby promoting popularization of the injection pumps and benefiting the majority of patients. Apart from the method of controlling an injection pump, the present invention further provides a control unit for the injection pump and an injection pump comprising the control unit correspondingly.
According to an aspect of the present invention, a method of controlling an injection pump for injecting preparations is provided. The method comprises: (1) acquiring a current first location preparation concentration value of an individual to be injected with a preparation at a current moment; (2) acquiring an expected concentration value in plasma at a predetermined next moment based on a first injection scheme; (3) acquiring a supposed injection value at a current moment based on a second injection scheme, an expected concentration value in plasma at the next moment, and a current first location preparation concentration value; (4) injecting a preparation into an individual using the pump according to the supposed injection value; and (5) repeating steps (1) to (4) until the time experienced from a first injection reaches a lasting time length of the first injection scheme.
Preferably, when there is no preparation for injection, a first location preparation concentration value is 0.
Preferably, in step (1), a current second location preparation concentration value at a current moment is further obtained, and in step (3) a supposed injection value at a current moment is also obtained based on the current second location preparation concentration value.
Preferably, when there is no preparation for injection, a second location preparation concentration value is 0.
Preferably, the first injection scheme is a long-acting or intermediate-acting injection scheme.
Preferably, the second injection scheme is a short-acting or rapid-acting injection scheme.
Preferably, the first location is a subcutaneous location.
Preferably, the second location is interstice.
Preferably, the preparation is insulin.
Preferably, the displacement distance of a pump is monitored in real time when a pump is used to inject a preparation into an individual. In this way, an injection amount of a pump can be controlled accurately.
According to another aspect of the present invention, a control unit for controlling an injection pump to inject a preparation is provided, which comprises: a current concentration value acquiring module, which is configured to obtain a current first location preparation concentration value of an individual to be injected with a preparation at a current moment; an expected concentration value acquiring module which is configured to obtain an expected concentration value at a predetermined next moment based on a first injection scheme; a supposed injection value acquiring module which is configured to obtain a supposed injection value at a current moment based on a second injection scheme, an expected concentration value at the next moment, and the current first location preparation concentration value; an injection instruction sending module which is configured to send instructions to inject a preparation into an individual using the pump according to the supposed injection value; and a time counting module which is configured to send out a signal to stop injection when the time experienced from the first injection reaches the lasting time length of the first injection scheme.
Preferably, the expected concentration value acquiring module further acquires a current second location preparation concentration value at the current moment, and the supposed injection value acquiring module further acquires a supposed injection value at the current moment based on a current second location preparation concentration value.
Preferably, a time counting module is achieved through a counter, wherein when the times of predetermined injection are achieved, the time counting module sends out a signal to stop injection.
Preferably, a control unit further comprises a displacement transducer for monitoring a displacement distance of a pump in real time.
According to a further aspect of the present invention, an injection pump for injecting a preparation is provided, the injection pump comprising a pump body and a control unit as stated in the previous paragraph, wherein the control unit is arranged in the pump body.
According to an aspect of the present invention, a method of controlling an insulin injection pump is provided. The method comprises: (1) obtaining a current subcutaneous insulin value and a current interstitial insulin value of an individual to be injected with insulin at a current moment; (2) obtaining an expected plasma insulin concentration value at a predetermined next moment based on a first insulin injection scheme; (3) calculating a supposed injection value at a current moment based on a second insulin injection scheme, a current subcutaneous insulin value, a current interstitial insulin value, and an expected plasma insulin concentration value at a next moment; (4) injecting insulin into an individual using the pump according to the supposed injection value; and (5) repeating steps (1) to (4) until the time experienced from the first injection reaches a lasting time length of the first insulin injection scheme.
Preferably, when there is no insulin injection, the subcutaneous insulin value and the interstitial insulin value are 0.
Preferably, a current subcutaneous insulin value at a current moment is obtained first, and then a current interstitial insulin value is obtained based on the current subcutaneous insulin value.
Preferably, an insulin value refers to a monomeric/dimeric insulin value.
Preferably, a first injection scheme is a long-acting or intermediate-acting injection scheme.
Preferably, a second injection scheme is a short-acting or rapid-acting injection scheme.
Preferably, when an individual is injected with insulin using the pump, the displacement distance of the pump is monitored in real time. In this way, the injection amount of a pump can be accurately controlled.
According to another aspect of the present invention, a control unit for controlling an insulin injection pump is provided, the control unit comprising: a current concentration value acquiring module, which is configured to obtain with a current interstitial insulin value and a current subcutaneous insulin value at a current moment; an expected concentration value acquiring module, which is configured to obtain an expected plasma insulin concentration value at a predetermined next moment based on a first insulin injection scheme; a supposed injection value acquiring module, which is configured to obtain a supposed injection value at a current moment based on a second insulin injection scheme, an expected plasma insulin concentration value at the next moment, a current subcutaneous insulin value and a current interstitial insulin value; an injection instruction sending module, which is configured to send out an instruction to inject insulin into the individual using the pump according to the supposed injection value; and a time counting module, which is configured to send out a signal to stop injection when the time experienced from the first injection reaches the lasting time length of the first injection scheme.
Preferably, the time counting module is realized through a counter, wherein when a predetermined injection times are achieved, the time counting module sends out a signal to stop injection.
Preferably, a control unit further comprises a displacement transducer for monitoring a displacement distance of a pump in real time.
According to a further aspect of the present invention, an injection pump for injecting insulin is provided, the injection pump comprising a pump body and the control unit as stated in the previous paragraph, wherein the control unit is arranged in the pump body.
According to an aspect of the present invention, a method of controlling an insulin injection pump is provided. The method comprises: (1) obtaining a first expected plasma insulin concentration value of an individual to be injected with insulin at a predetermined first moment based on a first insulin injection scheme; (2) calculating an initial supposed injection value at an initial moment based on a second insulin injection scheme and the first expected plasma insulin concentration value; (3) injecting insulin into an individual using the pump according to the initial supposed injection value; (4) obtaining a first moment interstitial insulin value and a first moment subcutaneous monomeric/dimeric insulin value at the first moment based on a second insulin injection scheme; (5) obtaining a second expected plasma insulin concentration value at a predetermined second moment based on the first insulin injection scheme; (6) calculating a first supposed injection value at a first moment based on the second insulin injection scheme, the second expected plasma insulin concentration value and the first moment subcutaneous monomeric/dimeric insulin value and the first moment interstitial insulin value; (7) injecting insulin into an individual using the pump according to the first supposed injection value; and (8) obtaining a supposed injection value at each moment by iteration based on steps (4) to (7) and injecting insulin into the individual using the pump according to the supposed injection value until the time experienced from a first injection reaches a lasting time length of the first insulin injection scheme.
Preferably, an interval between a first moment and an initial moment is equal to an interval between a second moment and the first moment.
Preferably, the time interval between every two injections is fixed.
Preferably, the first injection scheme is a long-acting or intermediate-acting injection scheme.
Preferably, the second injection scheme is a short-acting or rapid-acting injection scheme.
According to another major aspect of the present invention, a control unit for controlling an insulin injection pump is provided. The control unit comprises: an expected plasma insulin concentration value acquiring module, which is configured to obtain a first expected plasma insulin concentration value at a predetermined first moment and a second expected plasma insulin concentration value at a predetermined second moment of an individual to be injected with insulin based on the first insulin injection scheme, until an N-th expected plasma insulin concentration value at a predeteiuiined N-th moment is reached; wherein N refers to the number of times of predetermined injections; a current subcutaneous and interstitial insulin concentration value acquiring module, which is configured to obtain with a current subcutaneous insulin value and a current interstitial insulin value at a current moment; a supposed injection value acquiring module, which is configured to obtain a supposed injection value at a current moment based on a second insulin injection scheme, an expected plasma insulin concentration value at a next moment, the current subcutaneous insulin value and the current interstitial insulin value; an injection instruction sending module, which is configured to send out an instruction to inject insulin into an individual using the pump according to the supposed injection value; and a counting module, which is configured to send out a signal to stop injection when the time experienced from the first injection reaches the lasting time length of the first injection scheme.
Preferably, a time counting module is achieved through a counter, wherein the time counting module sends out a signal to stop injection when predetermined injection times are accomplished.
Preferably, a control unit further comprises a displacement transducer for monitoring a displacement distance of a pump in real time.
According to a further aspect of the present invention, an injection pump for injecting inulin is provided, the injection pump comprising a pump body and the control unit as stated in the previous paragraph, wherein the control unit is arranged in the pump body.
It should be understood that the technical solution of the present invention is not only limited to the abovementioned as listed. The features of all the abovementioned major technical solutions and all the preferable schemes can be combined freely as long as the combined features are not conflicting with each other therebetween.
The specific embodiments of the present invention are set forth hereinafter in combination with the drawings. It should be understood that these embodiment modes are illustrative rather than restrictive.
The present invention is described using some examples in the description, wherein some preferable embodiment modes are included. It should be understood that the scope of protection of the present invention further comprises other examples.
For example, a system and method may comprise a data signal transmitted via a network (e.g., a local area network, a wide area network, an Internet, and their combination, etc.), an optical fiber medium, a carrier, a wireless network or the like for communicating with one or more data processing units. The data signal can carry any or all data disclosed in the text. In addition, the method and system described herein can also be realized on different types of processing units by executing program codes comprising instructions on processing subsystems of these units. Software program instructions may comprise source codes, target codes, machine codes or other stored data, which can be operable to enable the processing system to execute the method and operation described herein. Other embodiment modes are also feasible, e.g., firmware or even suitable hardware that can execute the method and system as described in this text is arranged.
Data (e.g., relevance, mapping, data input, data output, intermediate data results, final data results and the like) of the system and method can be stored and executed on one or more different types of non-volatile computer readable storage media, wherein the media can be in a certain location or distributed in different locations. The media may comprise data storage units that are implemented by computers, e.g., different types of storage units and programming structures (e.g., RAM, ROM, flash memory, flat files, database, programming data structures, programming variables, IF-THEN (or similar) declaration structures, etc.). It should be understood that data structure describes format for organizing and storing data, database, programs, memory or other computer readable media that can be used by computer programs.
The system and method can be provided on many different types of computer readable media, including computer storage mechanical structures (e.g., CD-ROM, disks, RAM, flash memory, computer hard disk and the like) which comprise instructions (e.g., software) to be executed by processors so as to complete the operation of the method described herein and realize the system.
The computer components, software modules, functions, data storage and data structures described herein can be directly or indirectly connected to one another to allow data flow required in the work. It should be further understood that the module or processor not only comprises such code units that execute software operations, but also can be realized as a subprogram unit, as a software functional unit of codes, as an object (in an object-oriented normal form), as an applet, in a computer script language, or as another type of computer codes. The software components and/or functions can be located on a single computer or distributed on a plurality of computers according to different conditions.
It should be understood that the components used in the present description and mentioned in the claims can be in a single form or in a plural font'. Similarly, “in . . . ” as used in the present description and mentioned in the claims comprises two meanings, i.e., “in . . . ” and “on . . . ” unless specifically indicated in the context. Finally, “and” and “or” as used in the present description and mentioned in the claims comprise two meanings, i.e., connective and separated unless specifically indicated in the context. They can be used interchangeably. The term “exclusive or” can be used to indicate the condition of using the meaning of separation only.
It should be understood that in other embodiments of the present invention, it is possible to only obtain a preparation concentration value at a first location or at a second location, rather than obtain both preparation concentration values at a first location and a second location.
In a preferred embodiment mode, the first location can be a subcutaneous location, and the second location can be interstice. The first injection scheme is a long-acting injection scheme, and the second injection scheme is a rapid-acting injection scheme, with insulin as the preparation.
In particular, with respect to insulin injection, the present invention can be transformed into rapid-acting injection scheme that is suitable for injection using an injection pump based on pharmacokinetic principles according to conventional prescriptions of insulin, in which clinical prescription schemes and clinical experience of insulin acquired by doctors are fully utilized to increase accuracy and reliability of the prescriptions. The method of using the pump is simplified, the time of setting and adjusting dosage is reduced, and the time spent on using insulin for doctors and patients is saved. Therefore, insulin is taken as an example hereinafter to describe a particularly specific embodiment mode of the present invention.
At present, there are various types of insulin that are used clinically. The usual mode of administration is subcutaneous injection, but the effect features during action differ significantly. The short-acting insulin (RI) takes effect from 15 to 60 minutes after injection, the rapid-acting insulin from 10 to 15 minutes, the intermediate-acting (NPH, lent) insulin from 2.5 to 3 hours, and the long-acting (ultralente, glargine) insulin from 2 to 3 hours, wherein the effect can last for 20 to 30 hours. The major factor that causes differing time of action lies in the difference in the process of subcutaneous absorption of insulin, which process generally comprises the following stages: in subcutaneous tissues, crystalline insulin is dissolved into hexamer which can be depolymerized into dimer/monomer. The latter can be absorbed into blood after entering into interstice subcutaneously. Each stage of absorption follows a first-order kinetic mode.
In this example, it is set that a conventional therapeutic solution is 10IU NPH/day.
Moreover, the patient weighs 70 kg, and rapid-acting insulin, e.g., NovoRapid from Novo Nordisk, is used in an insulin pump.
First of all, the pharmacokinetic features of intermediate-acting insulin NPH (e.g., Novolin from Novo Nordisk) are calculated.
The process of subcutaneous absorption of NPH is described using differential equations:
Analytical solutions of plasma NPH concentration IN with respect to time t can be obtained by solving the abovementioned equation set:
I
n=exp(−(4*t)/25)*((1022699*exp((24*t)/200))/163961000+(50681673*exp((793*t)/5000))/4936028500−(2182714053*exp((953*t)/10000))/494945696200−(4449021*exp((1511*t)/10000))/327887000+1682730209/1141906519000) (8)
The insulin concentration in plasma at any moment after one shot of injection of the intermediate-acting insulin can be obtained according to the equation above.
Next, the pharmacokinetic features of rapid-acting insulin are calculated.
The process of subcutaneous absorption of rapid-acting insulin is described using differential equations:
With respect to other parameters, reference can be made to the previous text.
The following equations can be obtained by solving the abovementioned equation set:
x
dmt
=x
dm0*exp(−(53*t)/5000) (10)
x
it=(20*exp(−(321*t)/5000)*((613*xi0)/20−(32489*xdm0)/5360+(32489*xdm0*exp((67*t)/1250))/5360))/613 (11)
I
mtexp(−(4*t)/25)*((32489*xdm0)/7156260−(613*xi0)/9580+Im0−exp((479*t)/5000)*((32489*xdm0)/2567440−(613*xi0)/9580)+(32489*xdm0*exp((747*t)/5000))/4003920) (12)
wherein,
The amount of subcutaneous and interstitial insulin and the insulin concentration in plasma at any moment after one shot of injection of the rapid-acting insulin can be respectively calculated through the abovementioned equations.
Next, an injection amount of an insulin pump at each time point is calculated.
The amount of injecting rapid-acting insulin with a pump can be calculated by a cyclic iterative method to analyze the equation set composed of equations (10) to (12) according to the concentration values of the intermediate-acting insulin NPH at each time point. Supposing that the pump injects once every three minutes, the specific calculation method is as follow:
(a) it is calculated that the plasma concentration of intermediate-acting insulin within three minutes is IN3=0.07987 mU/L according to equation (8);
(b) the amount of rapid-acting insulin in each location at the moment of 0 is obtained; supposing that the rapid-acting insulin U0 is injected at the moment of 0, then the amount of the instantaneously subcutaneous rapid-acting insulin when insulin U0 is injected at the moment of 0 is xdm0+=U0; at this moment, the rapid-acting insulin has not yet arrived in the interstice and the plasma, so xi0=0 in the interstice, and Im0=0 in plasma;
(c) if it is desired that the rapid-acting insulin concentration in the 3rd minute reaches the intermediate-acting insulin level at this moment, then Im3−IN30.07987 mU/L. If xdm0+=U0, xi0=0, Im0=0, Im3=0.07987 mU/L are substituted into equation (12), and a value of 3min is assigned to t, a following equation can be obtained:
0.07987 mU/L=exp(−(4*3)/25)*((32489*xdm0+)/7156260−(613*0)/9580+0−exp((479*3)/5000)*((32489*xdm0+)/2567440−(613*0)/9580)+(32489*xdm0+*exp((747*3)/5000))/4003920)
In this equation, only xdm0+ is unknown, the equation is solved to obtain xdm0+=343.994 mU, U0=xdm0+=343.994 mU, i.e., the injection amount of insulin at moment 0 is 343.994 mU;
(d) the amount of rapid-acting insulin at each location in the 3rd minute is sought; it is recorded that the subcutaneous rapid-acting insulin before injection for the second time using the pump in the 3rd minute is in an amount of it can be obtained that xdm3−=333.227 mU according to equation (10); it is recorded that the amount of interstitial rapid-acting insulin in the 3rd minute is xi3, and it is obtained that xi3=9.789 mU according to equation (11); and it can be derived from step (c) that Im3=0.079874 mU/L;
(e) according to equation (8), it is calculated that the plasma concentration of the intermediate-acting insulin in the 6th minute is IN6=0.2605 mU/L;
(f) let the amount of injecting rapid-acting insulin in the 3rd minute be U3, then xdm3+=xdm3−+U3=333.227 mU±U3; it can be derived from step (d) that xi3=9.789 mU, Im3=0.079874 mU/L; it is calculated from step (e) that the plasma concentration of rapid-acting insulin after 3 minutes (i.e., in the 6th minute) should be Im6=IN6=0.2605 mU/L. The plasma insulin concentration in the 6th minute is calculated on the basis of the amount of rapid-acting insulin at each location in the 3rd minute, and substituted into equation (12) to obtain the equation
0.2605 mU/L=exp(−(4*3)/25)*((32489*(333.227 mU+U3))/7156260−(613*9.789 mU)/9580+0.079874 mU/L−exp((479*3)/5000)*((32489*(333.227 mU+U3))/2567440−(613*9.789 mU)/9580)+(32489*(333.227 mU+U3)+*exp((747*3)/5000))/4003920)
in which only U3 is unknown and the equation is solved to obtain U3=20.2026 mU, i.e., 20.2026mu should be injected in the 3rd minute, and xdm3+=xdm3−+U3=353.4296 mU after injection;
(g) steps (d) to (f) are repeated to calculate the injection amount of the insulin pump every three minutes. Reference can be made to Table 1, wherein the injection amount of the rapid-acting insulin of the insulin pump at each time point is displayed.
In order to verify the coincidence of plasma concentrations of one shot of injecting both rapid-acting insulin and intermediate-acting insulin using the pump, the plasma concentrations of one shot of injecting both the rapid-acting insulin and the intermediate-acting insulin using the pump are respectively calculated every six seconds. Results show that the difference value therebetween is less than 1% after one minute, and is less than 0.1% after three minutes.
According to the examples above, the injection amounts of the pump at respective time points can be calculated so as to realize transformation of a scheme of injecting intermediate-acting or long-acting insulin into a scheme of injecting rapid-acting insulin. In other embodiment modes, it can be realized that an injection scheme of intermediate-acting or long-acting insulin is transformed into an injection scheme of short-acting insulin, or that transformation of other injection schemes can be realized. Table 2 shows kinetic parameters of various insulin that are used for simulation. Table 2 shows kinetic parameters of various types of insulin products, wherein the values thereof are medians and can be used for reference.
In the abovementioned examples, the amounts of rapid-acting insulin in various locations can also be obtained without equations, but measured through proper detection methods (e.g., ACS 180PLUS); the injection interval can also be set as other suitable values, e.g., two minutes, five minutes or ten minutes, rather than 3 minutes.
In a further example, the displacement distance of a pump can be further monitored in real time using, for example, a displacement transducer, thereby monitoring an injection amount to achieve the purpose of accurate injection.
In some other embodiment modes of the present invention, the injection schemes of other preparations can also be transformed to obtain injections schemes that are more beneficial for use in the injection pumps, thereby providing great convenience to patients.
The time counting module 205 can be realized through, e.g., a system clock of the control unit. It can also be realized through, for example, a counter; then in such circumstance, when the number of times of predetermined injections is reached, the counter sends out a signal to stop injection. In some embodiment modes, let the time interval between each injection be unchanged, then the number of times of predeteimined injections can be obtained using a division method simply based on the duration of the first injection scheme.
It should be understood that
The present invention is applied to an SD rat used for experiment. The injection accuracy of the injection pump in an animal experiment is studied in the present invention, feasibility of the present invention is further discussed by comparing the pharmacokinetic curve between the injection accuracy and subcutaneous injection of insulin.
Method: SD rats are used and STZ is used for once large dose injection into an abdominal cavity. After one week, a blood glucose level is detected. If it is greater than 11.6 mmol/L, then it is prompted that a T1DM rat model is successfully established. The T1DM rats are randomly divided into an NPH single subcutaneous injection group, an RI injection pump continuous subcutaneous injection group, and a blank control group. During the 4-hour experiment, sampling is conducted at each blood collection point and insulin concentration thereof is detected, respectively. The injection amount of the injection pump is also measured every 15 minutes according to the scheme and compared with the theoretical expected value.
1.1 The strain comes from T1DM diabetic SD rats purchased from Nanjing BetterBiotechnology Co., Ltd.
1.2 Gender and Week Age: male, 7-8 weeks.
1.3 The feeding environment is at the temperature between 20 and 25° C., in a humidity between 40% and 70% or so. The rats eat and drink freely, and the rat cage is cleaned regularly.
There are totally 8 injection pumps used in the experiment, which are encoded with P001 to P008 and produced by Shanghai Zensun Science and Technology Development Co., Ltd.
Novolin R Cartridge 100 IU/ml, Novo Nordisk (China) Pharmaceutical Co., Ltd; Novolin N Cartridge 100 IU/ml, Novo Nordisk (China) Phamiaceutical Co., Ltd.
After ambrosia for 24 hours of SD rats that weigh 200 g, the dosage of 70 mg/kg is respectively administered to each group through once abdominal injection.
Model Forming Standard: the rat tail venous blood glucose value is measured using a glucometer after 72 hours from administration, a random glucose value equal to or greater than 11.6 mmol/L serves as a model forming standard for diabetic rats. The random glucose value that is actually measured after molding is equal to or greater than 20mmo1/L.
After the model is successfully established, T1DM rats are randomly divided into NPH single subcutaneous injection group, an injection pump using group, and a blank control group. As for the NPH single subcutaneous injection group, Novolin N is used for single subcutaneous injection, and administered in a dose of 5 IU/kg; in the RI injection pump continuously subcutaneous injection group, an NPH sample of Novolin R in the kinetic progress is administered for subcutaneously continuous pumping; the black control group is administered and injected with normal saline using the pump for subcutaneously continuous pumping.
Blood samples are collected and the insulin amounts are detected 30 minutes, 45 minutes, 60 minutes, 75 minutes, 90 minutes, 105 minutes, 120 minutes, 135 minutes, 150 minutes, 180 minutes and 240 minutes after treatment by administration. A Roche electrochemical luminescence instrument cobas e601 is used for insulin detection, which is produced by a Roche company of diagnosis products and gauged by Guangzhou Kingmed Medical Detection Center.
The injection amounts of the injection pump every 15 minutes in the experiment are substantially consistent with theoretical expectations, see
After using the injection pump of the present patent, the curves of insulin in vivo concentration can be basically controlled as time changes.
The present invention is set forth above through specific embodiment modes. It should be understood that the abovementioned descriptions are descriptive rather than restrictive. For example, the features of the abovementioned contents can be used solely, or can be used in any combination as long as they do not go beyond the scope of the present invention. Moreover, without departing from the spirit of the present invention, amendments can be made to the embodiment modes to adapt to specific circumstances. Although the specific elements and process in the text define various embodiment modes, they are not restrictive but demonstrative. By reading the abovementioned description, many other examples would be obvious to persons skilled in the art. Thus, the scope of the present invention should be determined by considering the claims as well as the entirely equivalent scope covered by such kind of claims.
| Number | Date | Country | Kind |
|---|---|---|---|
| 201410709653.7 | Nov 2014 | CN | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/CN2015/094538 | 11/13/2015 | WO | 00 |
