Patent Grant
11425912
The instant application contains a Sequence Listing with 10 sequences which has been submitted via EFS-Web and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Apr. 12, 2017 named 35212_US_CRF_sequencelisting.txt, and is 17,111 bytes in size.
This application is the National Stage of International Application No. PCT/AU2015/000360, filed on Jun. 19, 2015, published in English under PCT Article 21(2), which claims the benefit of and priority to Australian Patent Application Serial No. 2014902374, filed on Jun. 20, 2014, the contents of which are incorporated by reference in their entirety.
The present invention relates to methods for enhancing at least one growth parameter of a leguminous plant via co-inoculation of a leguminous plant with at least one rhizobial microorganism together with at least one actinobacterial microorganism. In further aspects, the present invention also relates to leguminous plants co-inoculated with at least one rhizobial microorganism together with at least one actinobacterial microorganism, as well as specific actinobacterial strains and inoculant compositions which are useful in accordance with the present invention.
There are around 44 to 66 million tonnes of nitrogen fixed from atmospheric nitrogen by symbiosis of rhizobial microorganisms and legumes every year, which is nearly half of the nitrogen used in agriculture around the world.
The interaction of legume and rhizobia is highly specific and each rhizobial species has a distinct range of leguminous plant hosts for forming nodules and fixing nitrogen. Different steps of the nodulation process requires the exchange of various signals between host legumes and rhizobia.
Actinobacteria are a large group that includes different genera of Gram-positive bacteria with a high G-C content in their DNA. Actinobacteria are widely distributed in terrestrial environments and some, like the nitrogen-fixing symbionts Frankia, are known to form associations with plants through symbiotic relationships. Recent studies have also found that endophytic actinobacteria produced plant growth-promoting compounds such as indole-3-acetic acid (IAA) and siderophores.
Effects of actinobacteria on rhizobia and symbiosis with legumes have been noticed but not many studies have investigated this complex combination, although several studies have suggested antagonism occurring between actinobacteria and rhizobia.
For example, Antoun et al. (Canadian Journal of Microbiology 24: 558-562, 1978) disclosed antagonism tests between actinobacteria isolated from different soils and effective strains of rhizobia. These data demonstrated that some actinobacteria inhibit the growth of rhizobia in vitro and in planta. Specifically, thirty one percent of the 481 actinobacteria investigated inhibited two efficient rhizobia strains, Rhizobium meliloti A2 and S14.
In a further study, Damirgi and Johnson (Agronomy Journal 58: 223-224, 1966) disclosed that the number of nodules on soybeans inoculated with Rhizobium japonicum strains 122 and 123 in autoclaved soil were reduced by up to 35% and 53%, respectively, by treatment with the actinobacterium E8. They also isolated about 60 actinobacteria from one soil sample where there had been poor nodulation of clovers. However, 20 of 24 actinobacteria isolated from an experimental soybean field did not inhibit eight sensitive R. japonicum strains in in vitro tests.
Antagonism was also examined between actinobacteria and 12 strains of rhizobia from five soil samples by Patel (Plant and Soil 41: 395-402, 1974). It was reported that about 23-70% of the actinobacteria inhibited the rhizobia strains.
In light of the above, identification of actinobacterial inoculants that are compatible with rhizobial microorganisms and that can enhance the growth and development of leguminous plants would be desirable.
Nucleotide and amino acid sequences are referred to herein by a sequence identifier number (SEQ ID NO:). A summary of the sequence identifiers is provided below:
A sequence listing is also provided at the end of the specification.
In a first aspect, the present invention provides a method for enhancing at least one growth parameter of a leguminous plant, the method comprising co-inoculating the leguminous plant with:
wherein the co-inoculated leguminous plant has at least one enhanced growth parameter relative to a leguminous plant of the same taxon that has not been co-inoculated.
An “actinobacterial microorganism” as referred to herein should be understood to include any microorganism of the phylum Actinobacteria.
In some embodiments the actinobacterial microorganism is an endophytic actinobacterial microorganism. An “endophytic” actinobacterial organism should be understood to include any actinobacterial organism that is able to live within a plant for at least a part of its lifecycle without causing apparent disease. In some embodiments, an endophyte may also be able to complete its lifecycle in the absence of a plant host, and thus only be an opportunistic endophyte. In some embodiments, an endophytic actinobacterial microorganism refers to an actinobacterial microorganism which may be isolated from surface-sterilised healthy plant tissue. For reference, an example of isolation of actinobacterial endophytes from surface sterilised plant tissue is set out in Coombs & Franco (Appl. Environ. Micro. 69(9): 5603-5608, 2003).
In some embodiments, the actinobacterial microorganism is from a genus selected from Streptomyces, Microbispora or Micromonospora.
In some embodiments, the actinobacterial microorganism is from the genus Streptomyces.
In some embodiments, the actinobacterial microorganism is from a species selected from:
In some embodiments, the actinobacterial microorganism is from a species selected from Streptomyces ciscaucasicus, Streptomyces canus or Streptomyces rishiriensis.
In some embodiments, the actinobacterial microorganism comprises a 16S rRNA gene nucleotide sequence which is at least 90% identical to one or more of: SEQ ID NO: 3; SEQ ID NO: 4; SEQ ID NO: 5; SEQ ID NO: 6; SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9 and/or SEQ ID NO: 10.
In some embodiments, the actinobacterial microorganism comprises a 16S rRNA gene nucleotide sequence which is at least 90% identical to one or more of SEQ ID NO: 5 and/or SEQ ID NO: 6.
In some embodiments the actinobacterial microorganism comprises a 16S rRNA gene nucleotide sequence which is at least 90%, at least 91%, at least 91.5%, at least 92%, at least 92.5%, at least 93%, at least 93.5%, at least 94%, at least 94.5%, at least 95%, at least 95.5%, at least 96%, at least 96.5%, at least 97%, at least 97.5%, at least 98%, at least 98.1%, at least 98.2% at least 98.3%, at least 98.4%, at least 98.5%, at least 98.6%, at least 98.7%, at least 98.8%, at least 98.9%, at least 99%, at least 99.1%, at least 99.2% at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% at least 99.9% or 100% sequence identity to a comparison window of one or more of: SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9 and/or SEQ ID NO: 10.
When comparing nucleic acid sequences to calculate a percentage identity, the compared nucleic acid sequences should be compared over a comparison window of, for example, at least 100 nucleotide residues, at least 300 nucleotide residues, at least 600 nucleotide residues, at least 1000 nucleotide residues, at least 1100 nucleotide residues, at least 1200 nucleotide residues, at least 1300 nucleotide residues or at least 1400 nucleotide residues. In some embodiments, the comparison window may comprise the region in each of the compared nucleotide sequences between and including the binding sites of the 27f primer (SEQ ID NO: 1) and the 1465r primer (SEQ ID NO: 2) on the compared nucleotide sequences.
The comparison window may comprise additions or deletions (ie. gaps) of about 20% or less as compared to the reference sequence (which does not comprise additions or deletions) for optimal alignment of the two sequences. Optimal alignment of sequences for aligning a comparison window may be conducted by computerized implementations of algorithms such as the BLAST family of programs as, for example, disclosed by Altschul et al. (Nucl. Acids Res. 25: 3389-3402, 1997). A detailed discussion of sequence analysis can be found in Unit 19. 3 of Ausubel et al. (Current Protocols in Molecular Biology, John Wiley & Sons Inc, 1994-1998, Chapter 15, 1998).
A number of particularly useful actinobacterial microorganisms of the present invention have been deposited in accordance with the provisions of the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purposes of Patent Procedure.
The first deposited microorganism, referred to herein as Streptomyces sp. LuP30, has been deposited at the National Measurement Institute (NMI), Australia on 12 Dec. 2013 under accession number V13/030101.
Accordingly, some embodiments, the actinobacterial microorganism is Streptomyces sp. LuP30 as deposited with the National Measurement Institute, Australia under accession number V13/030101; or a mutant or derivative of said microorganism that retains the ability to enhance at least one growth parameter of a leguminous plant when the actinobacterial microorganism co-inoculated onto a leguminous plant with with a rhizobial microorganism.
The second deposited microorganism, referred to herein as Streptomyces sp. LuP47B, has been deposited at the National Measurement Institute (NMI), Australia on 12 Dec. 2013 under accession number V13/030100.
Accordingly, in some embodiments, the actinobacterial microorganism is Streptomyces sp. LuP47B as deposited with the National Measurement Institute, Australia under accession number V13/030100; or a mutant or derivative of said microorganism that retains the ability to enhance at least one growth parameter of a leguminous plant when the actinobacterial microorganism is co-inoculated onto a leguminous plant with a rhizobial microorganism.
In addition, in accordance with the present invention, two previously known organisms were also surprisingly identified to be particularly useful in accordance with the method of the present invention:
In some embodiments the actinobacterial microorganism is Streptomyces sp. EN23 as described in PCT publication WO/2005/003328 and deposited as AGAL Deposit No. NM03/35605, or a mutant or derivative of said microorganism that retains the ability to enhance at least one growth parameter of a leguminous plant when the actinobacterial microorganism is co-inoculated onto a leguminous plant with a rhizobial microorganism.
In some embodiments the actinobacterial microorganism is Streptomyces sp. EN27 as described in PCT publication WO/2005/003328 and deposited as AGAL Deposit No. NM03/35606, or a mutant or derivative of said microorganism that retains the ability to enhance at least one growth parameter of a leguminous plant when the actinobacterial microorganism is co-inoculated onto a leguminous plant with a rhizobial microorganism.
A “mutant or derivative” of the subject actinobacterial microorganisms should be understood to encompass, for example, any spontaneous or induced mutant, conjugation progeny or genetically modified form of the deposited strains which retains the ability to enhance at least one growth parameter of a leguminous plant when the actinobacterial microorganism is co-inoculated onto a leguminous plant with a rhizobial microorganism.
Mutagenisation techniques that may be used to generate derivatives or mutants include, for example, chemical mutagenesis (eg. EMS mutagenesis), ionising radiation-induced mutagenesis (eg. X-ray mutagenesis, γ-ray mutagenesis and UV mutagenesis), genetic insertion mutagenesis methods (eg. transposon mutagenesis) and the like.
As set out above, the present invention contemplates a method for enhancing at least one growth parameter of a leguminous plant.
A “growth parameter” of a leguminous plant, as referred to herein, can include any measurable characteristic of the leguminous plant.
In some embodiments, the growth parameter is a length and/or mass of a shoot of the leguminous plant.
In some embodiments, the growth parameter is a length and/or mass of a root of the leguminous plant.
In some embodiments, the growth parameter is a number and/or mass of nodules of the leguminous plant.
In some embodiments, the growth parameter is a number and/or mass of seed pods and/or seed produced by the leguminous plant.
In some embodiments, the growth parameter is a concentration and/or amount of a nutrient in the leguminous plant.
In some embodiments, the nutrient is selected from: Boron, Calcium, Copper, Magnesium, Manganese, Phosphorous, Sodium, Sulphur, Nitrogen and/or Zinc.
The concentration and/or amount of the nutrient may be measured using any method known in the art to be suitable for the relevant nutrient. Such methods may include, for example, the methods described by: Kirsten (Organic Elemental Analysis—Ultramicro, Micro and Traces Methods. Academic Press, New York, 1984); Horwath (Instrumental Organic Analylsis. Academic Press, New York, 1977); Colombo and Giazzi (American Laboratory 38-45, 1982); Fraisse and Schmidt (J. Microchem. 22: 109-130, 1977); Hegedus (Microchim. Acta 441-446, 1977); and Baur and Dirscherl (Microchim. Acta 1: 299-244, 1980).
In some embodiments, the nutrient is Nitrogen.
In some embodiments, the growth parameter is a germination rate of a leguminous plant seed. In some embodiments, “germination rate” may refer to the proportion of seeds of a leguminous plant that successfully germinate. In some embodiments “germination rate” may refer to a speed of germination of a leguminous plant seed, and/or a proportion of seeds of a leguminous plant that successfully germinate per unit time, for example the proportion of seeds of a leguminous plant that successfully germinate per 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19 or 20 days.
The “germination rate” of a leguminous plant seed may be assessed using any suitable laboratory based or field based method, as would be readily ascertained by those skilled in the art.
As set out above, the present invention contemplates “enhancement” of the one or more growth parameters of the leguminous plant. “Enhancement” of a growth parameter should be understood to include any improvement in a growth parameter in a co-inoculated leguminous plant relative to a leguminous plant of the same taxon that has not been co-inoculated in accordance with the method of the present invention.
In some embodiments, enhancement of a growth parameter will include an increase in the measured value of the growth parameter. For example, an increase in any of:
should be considered an enhancement of such growth parameters.
In some embodiments, enhancement of a growth parameter may comprise enhancement within a particular time period. For example, in some embodiments, enhancement of the growth parameter may comprise enhancement over a time period of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 60, 70, 80, 90 or 100 days.
In some embodiments, an “increase” in a growth parameter may include, for example, a 1%, 5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, 2-fold, 5-fold, 10-fold, 20 fold, 50-fold, 100-fold increase in the growth parameter in a co-inoculated leguminous plant relative to a leguminous plant of the same taxon that has not been co-inoculated.
In some embodiments, however, “enhancement” of the growth parameter may include a decrease in the measured value of the growth parameter. For example a decrease in the concentration and/or amount of a pathogen, disease symptom and/or toxin in the plant, and or a decrease in the time of germination of a leguminous plant seed, may be considered “enhancement” of such growth parameters.
In some embodiments, an “decrease” in a growth parameter may include, for example, a 1%, 5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100% decrease in the growth parameter in a co-inoculated leguminous plant relative to a leguminous plant of the same taxon that has not been co-inoculated.
As set out above, the present invention contemplates co-inoculating the leguminous plant with at least one rhizobial microorganism and at least one actinobacterial microorganism.
A “rhizobial microorganism” as referred to herein may include any microorganism that is capable of fixing nitrogen after becoming established in a root nodule of a leguminous plant.
Rhizobial microorganisms are a paraphyletic group that generally fall into two classes of the proteobacteria, the alpha- and beta-proteobacteria. Most rhizobial microorganisms belong to the order Rhizobiales, but several rhizobia occur in distinct bacterial orders of the proteobacteria.
Examples of rhizobial microorganisms include:
In some embodiments, the rhizobial microorganism is a Rhizobium spp., a Sinorhizobium spp. or Ensifer spp., or a Bradyrhizobium spp.
In some embodiments, the rhizobial microorganism is of the species Sinorhizobium meliloti or Sinorhizobium medicae. In some embodiments, the rhizobial microorganism is selected from the list of: S. meliloti strain RRI128 (referred to hereafter as ‘RRI128’), Sinorhizobium strain SRDI736 (referred to hereafter as ‘SRDI736’) or S. medicae strain WSM1115G (referred to hereafter as WSM1115G′).
In some embodiments, the rhizobial microorganism is of the species Rhizobium leguminosarum. In some embodiments, the rhizobial microorganism is selected from the list of: R. leguminosarum bv. viciae (referred to hereafter as ‘WSM1455’) or R. leguminosarum bv. trifolii (referred to hereafter as WSM1325′).
In some embodiments, the rhizobial microorganism is of the species Bradyrhizobium sp. lupini or Bradyrhizobium japonicum. In some embodiments, the rhizobial microorganism is selected from the list of: Bradyrhizobium sp. lupini strain WSM471 (referred to hereafter as ‘WSM471’) or Bradyrhizobium japonicum strain CB1809 (referred to hereafter as ‘CB1809’).
A range of rhizobial microorganisms are available from a range of commercial culture collections, as would be readily ascertained by those skilled in the art. In relation to a range of the rhizobial microorganisms described herein, these organisms can be accessed from the rhizobium culture collection of the South Australian Research & Development Institute (Plant Research Centre, Hartley Grove, Urrbrae SA 5064, Australia; www.sardi.sa.gov.au).
As set out above, the present invention contemplates a method for enhancing at least one growth parameter of a leguminous plant.
A “leguminous plant” as referred to herein should be understood as any member of the Fabaceae (or Leguminosae) that can form nodules when infected with a rhizobial microorganism.
Examples of leguminous plants include:
In some embodiments, the leguminous plant is a Medicago spp. plant. In some embodiments, the leguminous plant is a Medicago sativa, lucerne or alfalfa plant.
In some embodiments, the leguminous plant is a Trifolium sp. In some embodiments, the leguminous plant is a Trifolium subterraneum plant.
In some embodiments, the leguminous plant is Pisum sp. In some embodiments, the leguminous plant is a Pisum sativum plant.
In some embodiments, the leguminous plant is a Glycine sp. In some embodiments, the leguminous plant is a Glycine max plant.
As set out above, the present invention contemplates co-inoculating a leguminous plant with a rhizobial microorganism and an actinobacterial microorganism. As referred to herein, “co-inoculating” should be understood to include any method or process wherein a leguminous plant (including a leguminous plant seed) is brought into contact with a rhizobial microorganism and an actinobacterial microorganism. In some embodiments co-inoculation may comprise the rhizobial microorganism and/or actinobacterial microorganism being applied to a leguminous plant seed. Examples of leguminous plant seed inoculation are described by Hartley et al. (Crop and Pasture Science 63: 858-865, 2012). In some embodiments co-inoculation may comprise the rhizobial microorganism and/or actinobacterial microorganism being applied to soil in which a leguminous plant is growing. In some embodiments, co-inoculation may comprise the rhizobial microorganism and/or actinobacterial microorganism being applied to root and/or shoot tissue of a leguminous plant.
In some embodiments, “co-inoculating” may also comprise where the actinobacterial microorganism or rhizobial microorganism is pre-existing in the environment (eg. soil) into which a leguminous plant is grown. For example, co-inoculation may comprise application of an actinobacterial microorganism to a plant or soil and wherein a natural or pre-existing rhizobial microorganism in the soil co-inoculates the plant.
In some embodiments, the leguminous plant is exposed to a pathogen and, when exposed to the pathogen, the co-inoculated leguminous plant has at least one enhanced growth parameter relative to a leguminous plant of the same taxon that has not been co-inoculated.
In some embodiments, the pathogen is a root pathogen. A “root pathogen” as referred to herein should be understood to include any leguminous plant pathogen that infects and/or damages the roots of the leguminous plant. Examples of root pathogens of leguminous plants include fungal, oomycete, bacterial and/or nematode pathogens.
In some embodiments, the root pathogen is a nematode root pathogen such as a Pratylenchus spp., such as P. neglectus or P. thornei.
In some embodiments, the root pathogen is an oomycete root pathogen such as a Phytophthora spp., such as P. sojae.
In some embodiments, the pathogen is a fungal pathogen. Leguminous plants may be subject to attack by a range of fungal pathogens, including fungal root pathogens. Examples of such pathogens include Rhizoctonia spp., Pythium spp. or Aphanomyces spp.
In some embodiments, the pathogen is a Rhizoctonia sp. In some embodiments, the pathogen is Rhizoctonia solani. In some embodiments the pathogen is Rhizoctonia solani AGB.
In a second aspect, the present invention provides a leguminous plant, a leguminous plant part or leguminous plant reproductive material, co-inoculated with at least one rhizobial microorganism and at least one actinobacterial microorganism.
The leguminous plant contemplated in the second aspect of the invention may be any leguminous plant as hereinbefore described with reference to the first aspect of the invention.
Reference herein to a plant, plant part or plant reproductive material should be understood to encompass tissues, organs, whole organisms and parts thereof. In some embodiments, the term plant, plant part, or plant reproductive material should also be understood to encompass multicellular aggregations of cultured cells such as colonies, plant calli, suspension cultures and the like.
In some embodiments, the leguminous plant part or plant reproductive material may include a leguminous plant seed. As referred to herein, a plant “seed” should be understood to refer to a mature or immature plant seed. As such, the term “seed” includes, for example, immature seed carried by a maternal plant or seed released from the maternal plant. In some embodiments, the term “seed” may encompass any seed plant sporophyte between the developmental stages of fertilisation and germination.
In some embodiments, the actinobacterial microorganism contemplated in accordance with the second aspect of the invention is an actinobacterial microorganism as hereinbefore described with reference to the first aspect of the invention.
In some embodiments, the rhizobial microorganism contemplated in accordance with the second aspect of the invention is a rhizobial microorganism as hereinbefore described with reference to the first aspect of the invention.
In a third aspect, the present invention provides an isolated actinobacterial microorganism as deposited with the National Measurement Institute, Australia under accession number V13/030101; or a mutant or derivative of said microorganism that retains the ability to enhance at least one growth parameter of a leguminous plant when the actinobacterial microorganism is co-inoculated onto a leguminous plant with a rhizobial microorganism.
In a fourth aspect, the present invention provides an isolated actinobacterial microorganism as deposited with the National Measurement Institute, Australia under accession number V13/030100; or a mutant or derivative of said microorganism that retains the ability to enhance at least one growth parameter of a leguminous plant when the actinobacterial microorganism is co-inoculated onto a leguminous plant with a rhizobial microorganism.
In some embodiments, the present invention provides any of the hereinbefore described isolated actinobacterial microorganisms when used according to the method of the first aspect of the invention.
In a fifth aspect, the present invention also provides an inoculant composition comprising an actinobacterial microorganism.
In some embodiments, the actinobacterial microorganism comprises an actinobacterial microorganism as hereinbefore described with respect to the first aspect of the invention.
In some embodiments, the inoculant composition further comprises a rhizobial microorganism. In some embodiments, the rhizobial microorganism comprises a rhizobial microorganism as hereinbefore described with respect to the first aspect of the invention.
In some embodiments, the inoculant composition comprises a carrier or additive. The carrier or additives used will depend on the nature of the inoculant composition. For example, the inoculant composition may be in the form of a liquid composition, a solid composition (such as a powder, pellet or granular composition) a seed coating or the like.
The inoculant compositions of the present invention may be adapted to be applied to a leguminous plant in any suitable way. For example, the inoculant composition could be adapted to be applied as a seed coating, applied as a solid or liquid composition to the foliage or roots of a plant, or applied as a solid or liquid composition to soil before, during or after sowing of a leguminous plant.
A range of useful carriers or additives would be readily apparent to those of skill in the art and may include, for example: one or more gums (including xanthan gum), clay or peat based carriers, one or more nutrients including carbon or nitrogen sources, one or more antifungal or antibacterial agents, one or more seed coating agents, one or more wetting agents and the like.
In some embodiments, the present invention provides the hereinbefore described inoculant composition when used according to the method of the first aspect of the invention.
The present invention is further described with reference to the following non-limiting examples:
Isolation and Identification of Endophytic Actinobacteria
Four different legumes including lucerne, pea, clover and medics were collected from different places and picked randomly at various stages of growth around South Australia. Different media used for isolation of endophytic actinobacteria were Humic acid Vitamin B agar (HV; Masayuki and Hideo, Journal of Fermentation Technology 65(5): 501-509, 1987), yeast extract-casein hydrolysate agar (YECD), tryptic soy agar (TSA) (Oxoid Limited, UK), tap water yeast extract agar (TWYE), all at pH of 7.2±0.2. Benomyl (DuPont Qualicon, Wilmington, Del. USA) was added to each agar medium at a final concentration of 50 μg·ml−1 to control fungal growth.
The plants were washed under running tap water to remove dust and soil attached to the roots and nodules. The roots and nodules were separated from the plants and air-dried overnight at room temperature. The dry roots and nodules were surface sterilized following the method of Coombs and Franco (Applied and Environmental Microbiology, Vol. 69: 5603-5608, 2003). The surface sterilization process started by washing with absolute ethanol for 1 minute, followed by 6 minutes in 4% NaOCl, 30 seconds in absolute ethanol and a final wash with autoclaved R.O. water.
Surface sterilized nodules were snipped out from the roots and crushed in 0.9% saline until forming a homogenous mixture. The nodule suspension was spread onto the surface of at least three different isolation media. The sterilized roots were air dried before being cut into approximately 1 cm fragments by a blade or scissors, and placed onto the different media plates. Plates were incubated at 27° C. and 37° C.
The plates were checked regularly at least once per week from the first week until new single colonies could not be found. When colonies appeared, they were transferred to half strength potato dextrose agar (HPDA) for purification. The single colonies were transferred onto three different media such as HPDA, oatmeal agar (ISP3) and mannitol soybean agar (MS) to distinguish them based on their different morphologies, colour and pigments produced (media recipes all per Atlas, Handbook of Microbiological Media, 1993).
Actinobacteria, Sinorhizobium meliloti and Lucerne Seeds
Lucerne seeds named ‘SARDI Ten’ and Sinorhizobium meliloti RR1128 (referred to as RR1128), which is a commercial inoculant for lucerne, were supplied by the South Australian Research and Development Institute (SARDI). Seeds chosen for planting were similar in size and weight. Five endophytic actinobacteria (EN2, EN16, EN23, EN27, EN46) which were isolated from healthy wheat root and demonstrated to benefit plant growth of some cereals (see Patent Cooperation Treaty publication WO2005/003328, incorporated herein by reference), together with 148 endophytic actinobacteria isolated from different legumes, were tested both in vitro and in planta.
Effects of Endophytic Actinobacteria on Germination of Lucerne—on Agar
Lucerne seeds were placed in Petri dishes (usually 2-3 times the required amount) and surface sterilized by the following method: 30 seconds in 70% (v/v) ethanol, 2-3 minutes in 3% (v/v) hypochlorite solution, rinsed three times in autoclaved R.O. water, remaining in the third rinse for 10 minutes then left under the laminar flow to dry. Five sterilized seeds were put on a McKnight's solution 1% agar plate with one drop of an isolated actinobacteria suspension (200-2000 cells) applied to each seed while the control seeds received one drop of 0.9% saline. The plates were left under a 14 hour light cycle per day at room temperature (20-30° C.) for 2 weeks. The number of germinated seeds and the length of roots were recorded.
Effects of Endophytic Actinobacteria on Germination of Lucerne—in Sandy Loam
Lucerne seeds were also sterilized as described above and the sandy loam was autoclaved at 121° C. for 15 minutes. Twelve percent moisture sandy loam was made by adding McKnight's solution before adding 300 g of sandy loam with 20 sterilized seeds to a small basket, 10 cm wide and 20 cm length. The seeds were sown with actinobacterial suspension applied on the top before slightly covering with sandy loam. The baskets were kept under a 14 hour light/10 hour dark cycle at room temperature (20-30° C.) for 2 weeks. The number of seeds germinated was recorded, and when germinated, the length of the roots was measured. A total of 148 well-sporulating actinobacteria were tested.
IAA Production
The ability of the endophytic actinobacteria to produce IAA was examined following the method of Khanma et al. (World Journal of Microbiology and Biotechnology 25: 649-655, 2009). A 6 mm diameter plug of actinobacteria which was grown on ISP2 for 5-7 days was transferred into 5 ml of yeast malt extract (YME) containing 0.2% L-Tryptophan. The broth was shaken at 125 rpm for 7 days at 27° C. before centrifuging 1 ml of broth at 11,000 rpm for 15 min. The mixture of 0.5 ml supernatant and 1 ml Salkowski reagent (12 g of FeCl3 per litre of 7.9 M H2SO4) was mixed well and kept in the dark for 30 minutes. The IAA production activity was measured using optical density (OD) at 530 nm. YME broth without L-tryptophan was used as the base line and pure IAA (Sigma) with different concentrations were used to make a standard curve.
Phosphate Solubilisation Activity
The phosphate solubilisation ability of selected isolates was detected following the method described by Beneduzi et al. (Applied Soil Ecology 39: 311-320, 2008). The actinobacteria isolates were inoculated on glucose yeast (GY) medium that contained 10 g of glucose, 2 g of yeast extract and 1.5% agar in 1 L of distilled water. Two solutions were added to the medium, the first was 5 g of K2HPO4 in 50 ml distilled water and the second solution was 10 g of CaCl2) in 100 ml of distilled water. These two solutions were autoclaved separately and added into the GY medium before pouring into plates. These two solutions changed the colour of the GY medium to white opaque showing the presence of insoluble calcium phosphate. A positive reaction was demonstrated by the presence of a clear zone in the area surrounding the isolates.
Antagonism Tests
Rhizobial strains were grown on yeast mannitol agar (YMA; Graham, Applied Microbiology 17(5): 769-770, 1969) plates or slants for 3-5 days before harvesting. The rhizobial strains were harvested and serially diluted in 0.9% saline. The OD at 600 nm of the rhizobial solutions was checked and the number of colony-forming units was counted following the method of Miles and Misra (Journal Hygiene 38: 732-749, 1938). At the same time 100 μl of these serial dilutions at different OD values were spread onto YMA plates and allowed to dry. Two plugs about 25 mm2 of each actinobacterial strain grown on International Streptomyces Project 2 (ISP2, Atlas, Handbook of Microbiological Media, 1993) medium for 7 days were placed on the surface of the inoculated YMA plates. The plates were incubated for 5-7 days at 27° C. and checked daily for antagonistic activity against the rhizobia. Rhizobia and the actinobacteria that were grown separately as pure cultures on YMA plates were used as negative controls. Streptomycin, vancomycin and were used as positive antibacterial controls. All treatments were replicated twice and incubated at 27° C.
Interaction Between Endophytic Actinobacteria and Rhizobium on Lucerne
Lucerne seeds were surface-sterilized as described above and sown in autoclaved pots. Each pot contained about 1 kg of sand and vermiculite mixture, and had two separate parts to allow easy drainage. Five and 148 endophytic actinobacteria isolated from surface-sterilized healthy wheat roots and surface-sterilized healthy root and nodules of four different legumes such as lucerne, pea, clover and medics, respectively, were coated on the surface of the lucerne seeds in a 0.3% (w/v) sterile xanthan gum solution. 100 ml of MQ water was added to each pot before planting the ten coated seeds. The top of the pot was covered with a thin layer of granulated plastic beads. Then, 200 mL of 1/80 McKnight's solution containing starter nitrogen (266 mg NH4NO per 20 L McKnight's solution) was gently added to each pot before covering with freezer bags and placing in the glasshouse. The number of seedlings was thinned down to four plants before adding 1 ml of rhizobium RR1128 around 108 CFU/ml at five days from planting. Every week 50 ml of nitrogen solution (1.2 g·L−1 of NH4NO3) was applied to each pot for nitrogen-treated plants and MQ water was added as required.
All treatments were replicated ten times completely randomized in the glasshouse, with the position of the pots changed randomly every week. The plants were harvested after the sixth to seventh week from sowing. The parameters examined were height and dry weight of the shoot, length and dry weight of the root, the number and dry weight of nodules per plant. Nodules were removed, counted and dried at 60° C. Dry weight of each nodule was calculated by dividing total nodule weight by total nodule number of two plants with five replicates.
SPAD 502 Readings, Total Nitrogen Analysis and N2 Fixed in the Shoots
During the sixth week, leaves of lucerne plants were measured by a SPAD 502 meter (Chlorophyll meter SPAD-502, Konica Minolta) designed to indicate the amount of chlorophyll present in plant leaves. The three biggest leaves were checked to get SPAD readings. Moreover, dry leaves of control plants (only treated with Sinorhizobium), and plants treated with rhizobium and EN23 and EN27, LuP30 and LuP47B harvested in the seventh week were analysed for the content of nitrogen and other elements. The leaves were dried at 60° C. for 48 h to obtain constant weight and were ground to about 1 mm in size for analysis.
N2 fixation associated with the shoots was calculated by the following equation:
N2fixed in the shoots=(% N*SDW)treatment−(% N*SDW)uninoculated
Nutrient Limitation Experiment
Lucerne seeds were surface sterilized and coated with actinobacteria as described above. Two coated seeds were sown in a 50 ml centrifuge tube containing 65 g of autoclaved washed sand and 10 ml of McKnight's starter N (0.133 mg) added five times less than compared with normal 0.665 mg per plant in pot assays. This was then covered by sand and plastic beads. The number of seedlings was thinned down to one seedling before inoculating with 108 CFU·ml−1 RR1128 suspension. The tubes were kept inside the growth chamber with a 12 h light and 12 h dark cycle. Water was supplied as required until 7 weeks.
DNA Extraction
In a sterile 1.5 ml eppendorf tube 10 μl of lysozyme was added to 500 μl of Tris-EDTA (TE) pH 7.4, before re-suspending 2-3 loops of actinobacterial cells into the mixture, which was then vortexed and spun later. The eppendorf tube was incubated at 37° C. for 60 minutes before adding 10 μl of proteinase K and 32.5 μl of 10% SDS and incubated at 55° C. for 60 minutes. Next, 100 μl of 5 M NaCl and 65 μl of CTAB/NaCl were added and the mixture was incubated at 55° C. for 10 minutes. Six hundred microlitres of phenolchloroform was added and the tube was left at room temperature for 30 minutes with intermittent shaking every 10 minutes. After centrifuging at 12,000 rpm for 15 minutes, the supernatant was transferred to a new sterile 1.5 ml eppendorf tube. An additional 500 μl of chloroform was added to the tube and left at room temperature for 15 minutes with mixing by inversion every 7-8 minutes before centrifuging at 12,000 rpm for 15 minutes. The aqueous phase was transferred into a new sterile 1.5 ml eppendorf tube before adding 20 μl of 10 mg·ml−1 RNAse and incubating at 37° C. for 60 minutes. Then 500 μl of chloroform was added and the tube left at room temperature for 15 minutes. After centrifuging at 12,000 rpm for 15 minutes, the supernatant was transferred to a new sterile 1.5 ml eppendorf tube. An additional 500 μl of chloroform was added and the tube left at room temperature for 15 minutes (with mixing by inversion every 7-8 minutes). After centrifuging at 12,000 rpm for 15 minutes, the supernatant was transferred to a new sterile 1.5 ml eppendorf tube (steps repeated). A 0.1× volume of 3 M Na Acetate (50 μl) and 3× volume of 100% ethanol (1 ml) was added to the tube before leaving at −20° C. overnight. The supernatant was removed carefully so as not to disrupt the pellet after centrifuging at the maximum speed 16,000 rpm for 15 minutes. The pellet was washed twice with 70% ethanol and dried by placing tubes in a heating block at 55° C. with the lids open for approximately 10 minutes. Finally, the pellet was re-suspended in 50 μl of sterile water.
PCR of 16S rRNA Gene
A master mixture was prepared as 1 μl of dNTPs (10 mM), 1 μl of DNA Taq polymerase (5 U/μl), 5 μl of ThermoPol buffer, 2 μl of 27f primer (AGAGTTTGATCCTGGCTCAG; SEQ ID NO: 1), 2 μl of 1465r primer (TACGGYTACCTTGTTACGACTT; SEQ ID NO: 2), 2 μl of DNA sample and 37 μl of injection water. PCR was performed by heating the PCR tubes at 94° C. for 2 minutes, followed by 40 cycles of 94° C. for one minute, 52° C. for one minute and 72° C. for two minutes, and 72° C. for 10 minutes. A 1.2% agarose gel containing 3 μl of GelRed (Biotium) in 40 ml agarose was used to separate the molecular weight of the PCR products. One microlitre of loading dye was mixed well with 2 μl of each PCR product before loading the gel, which was run in a running buffer 0.5×TBE at 70 V and 400 mA for 60 minutes. The products of PCR were sequenced by Macrogen, Korea. The resultant 16S rRNA sequences were compared to the GenBank database by using the National Centre for Biotechnology Information database (NCBI) BLASTN program, including the results of the highest matches for each isolate and the corresponding bit score and percentage identity.
225 endophytic actinobacteria were isolated from roots and nodules of pea, lucerne, clover and medic. 73 were from nodules and 152 were from roots. Based on their morphology, 126 cultures (56%) belong to the genus Streptomyces, 54 (24%) belong to Microbispora, 20 (8.89%) belong to Micromonospora and 25 cultures are as yet unidentified. Humid acid vitamin B agar (HV), yeast extract casein dextrose (YECD) and tap water yeast extract (TWYE) media successfully allowed growth of almost all of the isolates mentioned. 125 cultures were isolated from HV medium, 72 cultures were from TWYE, 26 were from YECD and one of them was isolated from TSA. There was not much difference in the number of cultures isolated at 37° C. and 27° C., which were 125 and 112 cultures, respectively. Eighty five cultures were isolated from roots of lucerne, while 65 isolates were from pea (42 from roots and 23 from nodules), 37 cultures were from clover (16 from roots and 26 from nodules) and 35 were from medics (12 from roots and 23 from nodules). Thirty two out of the 73 isolates from nodules were Streptomyces, 17 were Microbispora, 5 were Micromonospora and 19 were unidentified.
Fifty six of 148 cultures (38%) isolated from lucerne promoted germination of lucerne seeds and 27 (18%) isolates negatively affected germination of lucerne seeds in terms of number of seeds germinated and length of roots on agar plates. In addition, 39 of 148 cultures improved germination of lucerne seeds with the presence of rhizobium on sandy loam.
As shown in
Two concentrations of rhizobia were tested for antagonism with 14 endophytic actinobacteria; five non-legume-isolated and nine legume-isolated cultures. As shown in
As shown in
As shown in
Treatment with EN27 resulted in slight increases in height, fresh and dry weight of the shoot, and fresh and dry weight of the root. Although EN27 significantly reduced the number of nodules, the fresh weight of each nodule and the total dry weight of nodules per plant were higher than that of RR1128 only plants. Furthermore, EN27 also significantly increased the SPAD readings, nitrogen content (% N2) and total nitrogen per plant. EN23 and EN27 increased the amount of N2 fixed in the shoots, by 0.85 and 0.80 mg per plant, respectively, compared with the RR1128 only plants (
The interactions between rhizobium RR1128 and 148 cultures isolated from legumes were screened in terms of plant growth and nitrogen fixation. LuP47B and LuP30 showed beneficial effects on the symbiosis of rhizobium and lucerne, with increased height of shoot, mass of shoot and plant and nitrogen fixation per plant. As shown in
As shown in
As shown in
Fifty milliliter centrifugal tubes were used to screen for the biocontrol ability of actinobacterial strains against the fungal root pathogen Rhizoctonia. Forty five grams of autoclaved sandy loam were used at a 12% moisture content added as McKnight's starter nitrogen (266 mg of NH4NO3) solution. Two millet seeds infected with R. solani AG8 strain W19 were added at the top of the sandy loam, and a further 10 g of soil containing 12% moisture was added to cover the millet seeds. Two tubes without adding the infested millet seeds with the pathogen and without endophytic actinobacteria were as used as controls. The tubes were placed in a rack covered with aluminium foil and placed in a chamber for two weeks at 15° C. in the absence of light.
Lucerne seeds were surface-sterilized and pre-germinated with actinobacterial suspensions on autoclaved moist filter paper in petri dishes. When the roots were about 1-3 mm length they were dipped in 5 ml (to cover all the seeds) of the rhizobial suspension (approximately 108 cfu/ml) for 3 minutes. Two pre-germinated and coated seeds were transferred into each 50 ml tube and covered with 5 g of soil (12% moisture) and a layer of plastic beads. The tubes were kept at 15° C. in a growth chamber for 3 weeks. There were two replicates of each treatment, and MQ water was added as required. The number of seedlings that emerged, the length of root and root damage were recorded.
Results of the biocontrol assay are shown in
LuP3, LuP12A, LuP30, LuP47B, EN23, EN27, LuP8 and LuP44 were putatively identified as Streptomyces sp. by 16S rRNA gene sequencing.
The determined 16S rRNA gene sequences for each organism were as follows:
As shown in
The effects of three actinobacteria on the growth and symbiosis of lucerne and rhizobia was studied at three levels of NH4NO3 3 ppm, 25 ppm and 50 ppm.
The factorial experiment comprised (i) 3 strains of actinobacteria, (ii) ±inoculation with Sinorhizobium meliloti RRI 128 and (iii) 3 levels of soil NH4NO3. The pots were prepared and watered with a nitrogen deficient McKnight's solution supplemented with NH4NO3 to provide soil nitrogen of 3, 25 and 50 ppm. Plant seeds designated to rhizobia treatments were inoculated with a suspension (1 ml per plant containing 108 CFU) of rhizobia 6 days after sowing. Each treatment was replicated eight times. Pots were arranged in a completely randomised design in a greenhouse and plants four pots of each treatment were harvested at 4 and 7 weeks after inoculation with the S. meliloti RRI 128.
As shown in
As shown in
Regarding nutrient levels, in the absence of the S. meliloti RRI 128 EN23, LuP30 or LuP47B reduced significantly iron and copper in shoot plants after 7 weeks at both 25 mg and 50 mg/kgnitrogen NH4NO3 supply while sodium and molybdenum was increased. Total nitrogen in shoot plants were increased significantly with seeds coated with LuP47B at 50 mg N while EN23 and LuP30 did increase total amount of nitrogen shoot plant but not significant. The actinobacteria showed the best impact on the nutrient in shoot of lucerne at 25 mg N after 4 weeks inoculation with S. meliloti RRI 128 EN23. Three actinobacteria treatment plants had higher the content of iron, manganese, boron, copper, molybdenum, zinc, and macro elements such as calcium, potassium, phosphate and nitrogen.
Increasing S. meliloti RRI 128 dose concentrations resulted in slight increases number of nodules and of the growth of the plant. The number of nodules per plant increased from 4.3 to 7.0 and 8.8 nodules when the concentration of rhizobia was increased from 5×102 to 5×104 and 5×106 respectively (see
Streptomyces spp. LuP30 and LuP47B were added as spores to lucerne seed with a sterile 0.3% xanthan gum solution and air dried before sowing. Seeds were treated with S. meliloti RRI 128. The planting process was as described in EXAMPLE 10.
The nitrogen supplied was 15NH415NO3 (98%) with initial N concentration in soil (25 mg/kg 15NH415NO3). Plants were harvested after 10, 21 and 35 days after inoculation with the S. meliloti RRI 128. Nitrogen in shoot and root materials was analysed by mass spectrometry to determine the proportions of plant N derived from the atmosphere and soil.
The plants were harvested at three times—at 10, 21 and 35 days after inoculation with S. meliloti RRI 128. The effectiveness of LuP30 and LuP47B was re-confirmed by the increase of the shoot dry weight and the number of nodules after 21 and 35 days co-inoculation with S. meliloti RRI 128 (
Clover cultivar Campeda (Trifolium subterraneum L.), was chosen to examine the effects of the two actinobacteria LuP30 and LuP47B which have shown an increase in growth and nitrogen fixation of lucerne in previous experiments. Rhizobial strain Rhizobium WSM 1325 was inoculated on seeds of clover.
The factorial experiment comprised (i) two strains of actinobacteria (LuP30 and LuP47B), (ii) inoculation with rhizobia strain WSM1325 for clover. Growth of rhizobia and actinobacteria, plant growth media and nutrition, sowing and water supply were as described above. The concentration of NH4NO3 was supplied at 25 mg per kg of sand and vermiculite where the actinobacteria LuP30 and LuP47B showed increased plant growth and nitrogen fixation for lucerne plants. Eight replicates for each treatment with four pots each harvested at 4 and 7 weeks after inoculation with rhizobia.
Co-inoculation of LuP30 with WSM 1325 increased the number of nodules after 7 weeks and nodule mass after 4 and 7 weeks in clover (
The interaction between LuP30 or LuP47B on the growth of two rhizobia Rhizobium WSM 1325 and Bradyrhizobium WSM 471 was studied at three concentrations 104, 106 and 106 CFU·ml−1 (or 103, 105 and 107 cells on each agar plate) of the two rhizobial strains. The two actinobacteria LuP30 and LuP47B were grown on ISP2 for 7-10 days and agar plugs of the well grown cultures were placed onto the agar plates containing the three rates of rhizobia. The growth of the rhizobia was examined 5 to 14 days after adding the actinobacteria plugs.
LuP30 and LuP47B showed positive and non-antagonistic effects on the growth of both rhizobia (Rhizobium WSM 1325 and Bradyrhizobium WSM 471). At low concentrations of rhizobia, less than 107 CFU·ml−1 or 105 CFU·ml−1 on each agar plate, LuP30 and LuP47B promoted a visible increase in the growth of both rhizobia on YMA medium after 5 days incubation at 27° C. (
The overall aim of this experiment was to evaluate a range of endophytic actinobacterial strains on the growth of soybean plants to determine whether these strains have a broad leguminous plant host range. The results of the study of plants harvested 4 weeks after the addition of the Bradyrhizobium inoculum to the actinobacterial-treated plants showed that 4 of the 18 strains tested had significantly improved plant growth and/or nitrogen content of the soybean plants.
Materials and Methods
Soybean seeds (Glycine max cv. Djackal) were surface-sterilised and coated with spores of actinobacteria suspended in 0.3% (w/v) xanthan gum. Coated seeds (1 per pot) were sown into a pasteurised potting mix ˜1 kg (50:50 by volume of sand:vermiculite) contained in 1.25 L pots. 200 ml of nitrogen deficient McKnight's nutrient solution was applied at sowing and supplemented to provide 25 mg of NH4NO3 per kg of potting media. Pots were arranged in a randomised block design with 5 replicates of each treatment. Plants were harvested at 4 weeks and 7 weeks after inoculation with Bradyrhizobium strain CB 1809.
Treatments were nil control, rhizobia with no actinobacteria, and rhizobium plus each of the following Streptomyces strains: Str. EN23, Str. EN27, Str. LuP3, Str. LuP5, LuP8, Str. LuP10, Str. LuP12A, Str. LuP30, Str. LuP44, Str. LuP46B, Str. LuP47B, Str. LuP73B, Str. LuP75, Str. PG3, Str. PG4, Str. PP1, Str. PP9, CM23.
Parameters measured were:
Effectiveness of Bradyrhizobium CB 1809 on Nodulation and Plant Growth of Soybean
Inoculation with Bradyrhizobium CB 1809 in the absence of actinobacteria resulted in abundant nodulation (around 120 nodules per plant) and increased shoot and root dry weights confirming the effectiveness of the Bradyrhizobium strain with the cultivar Djackal. There were no obvious constraints to nodulation, in the testing system.
Effect of Actinobacteria on Plant Growth and Symbiosis of Soy Plants at 4 Weeks Post Inoculation
Data for four (isolated from lucerne root) of the 18 actinobacteria tested are presented, based on their positive effects. Thirteen of the strains did not affect any of the parameters measured. As shown in
As shown in
Effect of Actinobacteria on Plant Growth and Symbiosis of Soy Plants at 7 Weeks Post Inoculation
As shown in
Pea field trials were sown Hart (28 May) and Riverton (10 June) in South Australia (SA), and at Pimpinio (15 May) in Victoria. The trials were arranged in randomised complete block design with 3 replicates, each comprising an uninoculated control and 3 inoculation treatments. Treatments were applied to Kaspa field pea which was sown to achieve a seedling density of 50 plants/m2. The rhizobia treatment (Rhizobium leguminosarum bv. viciae strain WSM1455) was applied at approximately 100 fold the rate recommended commercially. The co-inoculation treatment comprised the rhizobia treatment co-inoculated with Streptomyces sp. strain Lup47B, which was applied as spores to the seed before sowing.
Six plants were sampled from each plot at approximately 8 weeks after sowing and nodule number and nodule dry weight per plant determined. An additional ten plant shoots were sampled from each plot in October/November, (late pod fill) and used to estimate shoot biomass, pod number per plant and to estimate the % N derived from fixation using the 15N natural abundance method. Plots were machine harvested to estimate grain yield and subsamples used for the determination of grain protein (Total N Leco, CSBP).
Those skilled in the art will appreciate that the invention described herein is susceptible to variations and modifications other than those specifically described. It is to be understood that the invention includes all such variations and modifications. The invention also includes all of the steps, features, compositions and compounds referred to, or indicated in this specification, individually or collectively, and any and all combinations of any two or more of the steps or features.
Also, it must be noted that, as used herein, the singular forms “a”, “an” and “the” include plural aspects unless the context already dictates otherwise. Thus, for example, reference to “a microorganism” includes a single microorganism as well as two or more microorganisms; “a leguminous plant” includes a single plant as well as two or more plants; and so forth.
Reference to any prior art in this specification is not, and should not be taken as, an acknowledgment or any form of suggestion that this prior art forms part of the common general knowledge in any country.
Throughout this specification, unless the context requires otherwise, the word “comprise”, or variations such as “comprises” or “comprising”, will be understood to imply the inclusion of a stated element or integer or group of elements or integers but not the exclusion of any other element or integer or group of elements or integers.
Reference is made to standard textbooks of molecular biology that contain methods for carrying out basic techniques encompassed by the present invention, including DNA restriction and ligation for the generation of the various genetic constructs described herein. See, for example, Maniatis et al, Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press, New York, 1982) and Sambrook et al. (2000, supra).
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| Number | Date | Country | |
|---|---|---|---|
| 20200029519 A1 | Jan 2020 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 15320301 | US | |
| Child | 16539696 | US |
