The present invention generally relates to the production of insect lures, and particularly relates to the use of harborage with a substrate and mesh in a system for harboring, feeding and harvesting insects for lure production.
Lures are employed by pest control entities to draw pests toward a trap or observation device. For example, meal lures appeal to a pest's appetite, while mating lures appeal to a pest's desire to mate. Harborage lures appeal to a pest's desire to find a safe space in which to reside and/or find a mate.
Lures may depend on the state of a pest. Females may be more attracted to a meal lure than a mating lure. On the other hand, males may be more attracted to a mating lure than a meal lure. Pests may be attracted to harborage lures at specific times in their circadian rhythms. Some of these lure attractions/times may be mutually exclusive.
Lures may be natural, synthetic or a blend of natural and synthetic. Synthetic lures are generally comprised of volatile chemical compounds, sometimes referred to as VOC's. Volatiles become gaseous in air, which facilitates their dispersal. Further, many VOC's are considered carcinogenic. In addition, synthetic lures seem to capture only a fraction of the elements that attract pests. Synthetic lures also yield by-products, including offensive odors.
Natural lures are produced directly by mammals, insects, plants, reptiles, birds, etc., and are harvested by a number of methods. One method may be tactile, which includes contact with surfaces that absorb chemicals, such as pheromones, directly from the source. Headspace vapors, which may also include pheromones, emanate from the source and are captured on absorbent materials within that space. Solids, gases and/or liquids that are produced, including feces, urine, saliva, etc., may be captured by absorbent materials. Solids, gases and/or liquids may also be extracted from the source by exposing and/or removing components, including by way of gland removal, digestive tract removal, grinding of body parts, etc. Natural lures may also be comprised of volatile chemical compounds.
Synthetic compounds can be blended with natural compounds to enhance or preserve the lure and/or compensate for missing elements. Natural pheromones generally perform better than synthetic lures. In fact, research literature and empirical testing reveals that synthetic lures do not perform as well as naturally-occurring lures.
Despite their poorer performance, synthetic lures are typically the preferred approach. Synthetic lures are often less expensive to produce than natural lures, because they are typically comprised of a combination of commercially-available chemicals. Synthetic lures are also better known to regulatory entities. Furthermore, handling and caring for live creatures, especially large numbers of live creatures, is considered highly complex and risky.
Embodiments of the present invention provide for a more effective lure for insects, such as bedbugs, where the lure includes natural components that are harvested using an efficient and scalable system that handles massive numbers of live insects.
According to some embodiments, a harborage for growing and harvesting insects includes a substrate and a mesh material. The substrate, when configured for growing and harvesting insects, has a width and a length and first and second edges extending along the length and separated by the width, where the length is more than an order of magnitude greater than the width. The substrate comprises absorbent material and is formed into at least one channel extending in a direction along the length of the substrate. The channel may have a depth that is substantially less than the width of the substrate, in some embodiments. The mesh material is disposed on the substrate and extends along substantially all of the length of the substrate. The mesh material comprises openings that are smaller than juveniles of a target insect for the harborage but that are sufficiently large to permit liquid feeding of the target insect through the mesh material. The mesh material is affixed along the substrate so as to prevent egress of insects residing between the mesh material and the substrate.
The substrate may be formed into two or more channels extending along the length of the substrate, and perpendicular to the width of the substrate, in some embodiments. First and second channels of the two or more channels may be separated by a rib between the first and second channels and extending along the length of the substrate. The mesh material may be affixed to the rib in at least an intermittent manner along the length of the substrate.
In some embodiments, the substrate, when configured for growing and harvesting insects, comprises two or more channels extending diagonally along and across the substrate, wherein each of the two or more channels is divided into a plurality of cells.
According to some embodiments, a harborage producing system includes this harborage disposed along a conveyor system and a plurality of insects disposed in and along the at least one channel of the harborage, between the substrate and the mesh material. The harborage producing system may be made up of a number of zones along the conveyor system for handling different steps in the production process. The harborage producing system may utilize various components, such as rollers, a feeder, a freezer, a cutter, a grinder, and/or a shredder.
Of course, the present invention is not limited to the above features and advantages. Indeed, those skilled in the art will recognize additional features and advantages upon reading the following detailed description, and upon viewing the accompanying drawings.
It has been observed that bed bugs, cockroaches, etc., rely heavily on pheromones. Pheromones can suggest that a space is safe from predators, that a space is near meal hosts, or that mating opportunities may be nearby. It has also been observed that these pheromones are produced in multiple ways, including secretions, excretions or other gaseous or solid emissions. It has also been observed that these pheromones are transferred to surrounding/nearby materials and/or environment in multiple ways. These ways may be tactile, i.e., involving physical contact, or via the absorption of vapors into headspace.
It has further been observed that adult female bedbugs (and possibly adult females of other insect types) may venture away from colonies to find refuge, whereas adult males often stay close to a base harborage. It has also been observed that adult egg-laying females may seek and take more meals than males due to their reproductive needs.
Consideration has been given to the need to lure bed bugs to a trap or sensor. One example sensor that may effectively use a lure is the automated insect monitoring system described in U.S. Patent Application Publication US 2016/02387347, the entire contents of which are incorporated herein by reference. However, to date there has been a lack of effective, commercial, off-the-shelf, lures. Synthetic lures are available, but they generally underperform relative to natural lures. Synthetic lures typically have inherent risks as well, such as offensive odors, VOC's or flammability.
Embodiments of the present invention, as detailed herein, provide for a more effective lure with natural components that are harvested from insects using a scalable system that handles massive numbers of live insects. The system is a cost-effective means of growing and harvesting a natural lure. This approach is applicable to bed bugs, but the various techniques and equipment described herein may also be used to derive natural lures from other pests including roaches, weevils, moths, etc.
Features of the techniques and systems described herein include a continuous harborage, suitable for large-scale manufacturing, that is to conducive to comfortable habitation, breeding and feeding. This harborage is fully enclosed and is used to maximize production of lure components by feeding and keeping colonies of insects healthy and productive, while maintaining environmental conditions to support rapid maturity. This approach also maximizes the harvesting of lure components. The substrate of this harborage is comprised of materials that capture pheromones, vapors, liquids, solids, etc., that can he harvested, packaged, and distributed for use as lures. Note that the term “substrate” as used herein is meant in the sense of a material on which a process occurs, in this case the process of growing insects and collecting the pheromones, vapors, liquids, that result from this process. Containment materials (e.g., plankton netting mesh) that form a portion of this harborage may also be capable of capturing lure components, and can also be harvested. Exuvia (cast-off outer skin), recently deceased insects, and other detached solids that remain in the harborage can also be harvested and/or integrated into the lure.
The harborage, in some embodiments, comprises a substrate made of absorbent material, such as paper or cloth, or a mixture thereof.
In the pictured embodiment, the top, or ceiling or roof, of the harborage may be made of a mesh 120. Some sides may also be made of the mesh 120. The substrate 150 may include openings that are sized to be smaller than the smallest resident of the substrate 150. The openings may also be sized to facilitate feeding. The, openings may be sized to permit atmospheric equalization, to prevent trapped humidity that might cause mold or other problems.
The mesh 120 may be adhered to the substrate 150 completely along the perimeter to prevent escape. The mesh 120 may also be adhered completely or intermittently along ribs 130. The substrate 150 may be configured to keep the ribs 130 aligned and integrated with the mesh 120. The substrate 150 may also be configured to facilitate movement of bugs between lanes 110, to equalize the distribution of bugs. For example, in some embodiments, there may be intermittent openings in the ribs 130, to allow insects to move from one channel or lane 110 to another.
The internal volume of the substrate 150 may include additional harborage material 140, as shown in
According to some embodiments, a harborage for growing and harvesting insects to form insect lures, such as harborage 100, includes a substrate and a mesh material, such as substrate 150 and mesh 120. The substrate, when configured for growing and harvesting insects (i.e., when not in a flattened or folded configuration prior to use), has a width and a length and first and second edges extending along the length and separated by the width, where the length is more than an order of magnitude greater than the width. The substrate comprises absorbent material and is formed into at least one channel extending in a direction along the length of the substrate. The channel may have a depth that is less than the width of the substrate, for example. The mesh material is disposed on the substrate and extends along substantially all of the length of the substrate. The mesh material comprises openings that are smaller than juveniles of a target insect for the harborage but that are sufficiently large to permit liquid feeding of the target insect through the mesh material. The mesh material is affixed along the substrate, e.g., in a substantially continuous manner, so as to prevent egress of insects residing between the mesh material and the substrate.
The substrate may be formed into two or more channels extending along the length of the substrate, and perpendicular o the width of the substrate, in some embodiments. First and second channels of the two or more channels may be separated by a rib between the first and second channels and extending along the length of the substrate. The mesh material may be affixed to the rib in at least an intermittent manner along the length of the substrate.
In some embodiments, the substrate, when configured for growing and harvesting insects, comprises two or more channels extending diagonally along and across the substrate, wherein each of the two or more channels is divided into a plurality of cells. An example of such an embodiment is shown in
The harborage may include additional absorbent harborage material disposed in and along the at least one channel, the additional harborage material comprising one or more of: shredded material; folded material; baffled material; segmented material; crumpled material; die-cut material; and honeycomb material.
Zone 1 is a harborage zone. This may be a climate-controlled region, in some embodiments. This zone may contain a large collection of bugs and the pheromones they produce. Bugs can age, feed, procreate and dwell comfortably in this zone. The length of Zone 1 is adaptable and, preferably, long enough, when the movement of the harborage is accounted for, to allow bugs to grow to adulthood and propagate, even if they hatch mid-way along the zone. Note that the feeding regimen may be designed to draw bugs closer to Zone 0. This can concentrate more meals upstream and maximize feeding frequency upstream. Bed bugs can move about 25 feet per hour—this speed of movement should be factored into the design of the system, i.e., with respect to the length of Zone 1 and the speed at which the :harborage moves through the system 300.
Zone 2 is a stress region. It is a climate-controlled region, where bugs can age, procreate and harbor. Bugs are encouraged to leave this zone to move back to Zone 1. Bugs are not fed in this zone. Bugs may be repelled in this zone (e.g., cold temperatures, sounds, scents, etc.). Most likely only the fittest bugs will move upstream to Zone 1, where they can be fed. Weak, unfortunate, and dead bugs will remain in this zone, however.
Zone 3 is a neutralization region, where harborage and its contents (e.g., bugs, pheromone-infused substrate, and containment material) move into a freezer. Additional mechanisms may be employed, including UV light, pesticide, dehumidification, etc. The freezer also stores neutralized harborage in a manner that minimizes degradation.
Zone 4 is a bulk process lure mix ref-lion. This may include mechanical operations, such as grinding, shredding, cutting, etc. This may also include chemical operations, such as extracting, embellishing, etc. The lure mix is treated to minimize the likelihood of problems for the end-user (e.g., mold).
Zone 5 is a region for packing, storing and shipping. This may include anything from bulk packaging to unit-dose packaging. The product is placed in appropriate storage and shipped in appropriate containers to its destination.
According to some embodiments, a harborage producing system, such as system 300, is disposed along a conveyor system and includes a plurality of insects disposed in and along the harborage, e.g., along at least one channel of the harborage, between the substrate and the mesh material. The harborage producing system also includes one or more rollers disposed across the harborage at a first end of a first zone extending along the harborage, where the one or more rollers are disposed so as to compress the harborage and thereby form a boundary preventing the live passage of insects past the first end of the first zone.
The harborage producing system may include one or more additional rollers disposed across the harborage at a second end of a first zone extending along the harborage, the second end of the first zone being separated from the first end of the first zone by a first zone length. The one or additional rollers are disposed so as to compress the harborage and thereby form a boundary preventing the live passage of insects past the second end of the first zone.
The harborage producing system may further include a freezer, where the harborage and conveyor system are disposed to pass the harborage through the freezer. The harborage producing system may also include a cutter, grinder, and/or shredder, the harborage and conveyor s being disposed to pass the harborage into the cutter, grinder, and/or shredder.
Insects that feed on blood use a feeding appendage, such as a proboscis, to penetrate one or more membranes. Many insects inject a substance that conditions the blood and/or desensitizes the flesh near the insertion point to not alert animal. The proboscis acts like a straw to draw blood into the insect's digestive system. The state of the flesh, which sometimes doubles as a lure, is warm (for warm-blooded creatures), typically 97° to 100° F. Flesh may emit a flesh-like scent, which is alluring to blood-eating insects. Flesh may also pulsate, such as from a beating heart, which may also be alluring or facilitate orientation.
Most lab-based approaches feed discrete, small populations. The feeders may be clear, cup-like containers with mesh on top and folded paper inside. The paper enables bed bugs to climb and harbor. The mesh contains the bugs, but allows a proboscis to penetrate for feeding. These containers typically house less than 2000 bugs, and this density is too small for producing lures. But, more importantly, this approach makes it very difficult to harvest the pheromone-infused substrate without killing the vast majority of the colony and laboriously separating live bugs, many barely visible to the naked eve,from the substrate. This approach also risks the escape of bugs.
In contrast, some of the embodiments described herein are intended to let only dead, lethargic, and old/weak bugs get compromised, when processed after Zone 2. Since males seem less prone venture to find new harborages, males may have a greater tendency to be compromised during the harvesting process. However, a colony needs only a small or minority fraction of its population to be adult males, and females can lay up to dozens of eggs per insemination
Bugs feed when the warmed blood 430 and membrane 420 contact or are very close to the mesh 510.
The design of the feeder 600 may include a water heater-circulator to warm one or more feeding vessel. Pipes guide heated water from the heater-circulator to the feeding vessels. The feeder 600 includes chassis that provide structural stability to the feeder 600. The chassis may have at least two chassis portions that are used to spread a membrane 620 taught across a width between the chassis portions. The chassis portions may be solid or may be tubes that are configured to provide heat to warm the blood 710 or other liquid meal. Heated water or other fluid may circulate in such chassis tubes. The chassis portions may be thermally-conductive and/or made of aluminum, glass, etc. Chassis tubes 610 are shown in
The chassis tubes 610 occupy an interior space within the membrane 620, which may form a membrane tube 650. The length and width of the membrane 620 can scale with the membrane tube's 650 diameter. The chassis tubes 610 may protrude past ends of the membrane tube 650. As shown by
The chassis tubes 610 may be mechanically coupled with one or more couplers 630 that hold both chassis tubes 610 on one or more ends. Each coupler 630 may have a mechanism to spread the chassis tubes 210 in order to vary membrane tightness. A return bend 640 of the chassis tubes 610 may also provide structural integrity at one end. In some cases, only one coupler 130 is used in combination with the return bend 640 or similar structure to vary the membrane tightness.
The membrane 620 forming the membrane tube 650 may be tightened to a preferred tightness by spreading the chassis tubes 610. The blood (or other liquid meal) 710 pools inside the membrane tube 650. The blood 710 is warmed due to contact with the chassis tubes 610, 810. The membrane tube 650 that contains the blood 710 may be formed by a synthetic membrane or a natural membrane, such as a sausage casing. The membrane 620 may be retained at ends with rubber bands, zip ties, clamps, additional membrane layers (e.g., folds), etc. The chassis tubes 610 may have angled or vertical bends to form ends of a blood pool boundary. For example, the membrane tube 650 may bend vertically to contain blood pool at each end. This may allow one or both ends of the membrane tube 650 to be above the blood pool. This may allow the membrane tube 650 to form a reservoir to replenish the blood from the pool that is consumed.
As mentioned above, the membrane utility may be less than 120 minutes, so it s important to simultaneously feed as many bugs as possible. The membrane 620 may suffer from dehydration, solidification of blood along the membrane's 620 wall or floor, settling of suspended solids, or perforations from bug proboscies. To address this issue, a feeder insert may be placed within the membrane tube 650, such as shown by feeder insert 920 in the cross-section of the feeder 600 illustrated by
The membrane 620 used to form the membrane tube 650 may also be used to form a membrane trough. This membrane trough may be formed similar o the membrane tube 650.
Feeder 1800 thus comprises a feeder body 1810, which may comprise a piece of molded or cast metal, in some embodiments. A feeder membrane 1820 is affixed to a first surface of the feeder 1800. In the illustration, the membrane 1820 is on a top surface of the feeder 1800, although it will be appreciated that the feeder 1800 may typically be oriented with the membrane 1820 down, when feeding insects. Underneath the member 1820 are several channels or reservoirs 1830, which may be filled with blood for feeding the insects.
It will be appreciated that elements of the body 1810 extend between the channels or reservoirs 1830—this facilitates both a limiting of the reservoir area, to avoid some of the problems discussed above, but also allows for improved warming of the blood, as a metallic body 1810 can communicate heat to the blood in the reservoirs more effectively with this arrangement. On the blood retaining side of the conductive partition, these extensions, which may be regarded as “heat fins,” also may provide two additional functionalities. First, these fins may provide positional support for the feeder membrane. The feeder membrane, when oriented as shown in
The systems 1400, 1500 may provide significant scalability in one or more dimensions. The feeding area varies with feeding vessel length and membrane tube diameter. A feeding area may be, for example, 4.75 cm×17.75 cm, or 84.3 cm2. This is almost four times the area of a typical lab feeder.
However, natural and synthetic sausage casings can come in lengths upwards of 20 meters. Therefore, feeding areas can be extended to yield significantly larger feeding areas. For example, a 4.75 cm×40 cm feeding area would be 190 cm2, which is nine times more than a typical lab feeder, and so on. In fact, natural and synthetic sausage casings can come in diameters upwards of 15 cm, resulting in even larger feeding areas.
The feeder capacity varies with feeding vessel length and membrane tube diameter Other factors contribute to the feeder capacity. Ignoring the potential reservoirs at both ends, and without the insert, a feeder 600 may support a capacity volume, for example, of 4.75 cm×17.75 cm×1 cm, or 84.3 cm3. This is about 12.4 times a typical lab feeder. Capacity increases by adding the vertical reservoirs at both ends. A feeder insert may reduce the blood pool capacity. In an example, a feeder insert 920 displaces about ⅔ of the blood pool, resulting in a blood pool capacity of 28.1 cm3. This is about 4.2 times the typical lab feeder. Also, natural and synthetic sausage casings can come in lengths upwards of 20 meters and thus feeder volumes can be extended to yield significantly larger capacities. Natural and synthetic sausage casings can also come in diameters upwards of 15 cm, resulting in even larger feeder volumes.
According to some embodiments, the harborage of a harborage producing system, such as system 1400 or 1500, is disposed along a conveyor system and includes a plurality of insects disposed in and along the at least one channel of the harborage, between the substrate and the mesh material. The harborage producing system may include one or more rollers disposed across the harborage at a first end of a first zone extending along the harborage, where the one or more rollers are disposed so as to compress the harborage and thereby form a boundary preventing the live passage of insects past the first end of the first zone.
The harborage producing system may include one or more additional rollers disposed across the harborage at a second end of a first zone extending along the harborage, the second end of the first zone being separated from the first end of the first zone by a first zone length, wherein the one or more additional rollers are disposed so as to compress the harborage and thereby form a boundary preventing the live passage of insects past the second end of the first zone.
The harborage producing system may further include a freezer, the harborage and conveyor system being disposed to pass the harborage through the freezer. The harborage producing system may also include a cutter, grinder, and/or shredder, the harborage and conveyor system being disposed to pass the harborage into the cutter, grinder, and/or shredder.
Advantages of the described system, include the ability to feed very large populations simultaneously, rapidly and with minimal labor. Zone 1 of a typical infinity harborage may be required to support in excess of one million bed bugs. The feeders 600 are designed to support this requirement. Considering the feeder areas and capacities alone, labor can be reduced by at least a factor of four, and upwards to a factor of twelve or more. These savings continue to increase with longer and/or wider feeders.
Other advantages include the ability to facilitate conservation and recovery of the blood or other liquid meal. The feeder insert minimizes the blood in the pool to what is essential for bugs to feed without damaging their proboscis. The openings at each end of the membrane tube allows new blood or liquid meal to be poured in. Any unused blood or liquid meal may be poured out. The removed liquid may be poured into a new feeding vessel.
Notably, modifications and other embodiments of the disclosed invention(s) will come to mind to one skilled in the art having the benefit of the teachings presented in the foregoing descriptions and the associated drawings. Therefore, it is to be understood that the invention(s) is/are not to be limited to the specific embodiments disclosed and that modifications and other embodiments are intended to be included within the scope of this disclosure. Although specific terms may be employed herein, they are used in a generic and descriptive sense only and not for purposes of limitation.
This application claims priority from U.S. Provisional Application No. 62/310,846, filed Mar. 21, 2016, the entire contents of which are incorporated herein by reference.
Number | Date | Country | |
---|---|---|---|
62310846 | Mar 2016 | US |