Claims
- 1. A method of making a receptor polypeptide which is structurally and functionally related to the steroid/thyroid superfamily of receptors, wherein said receptor polypeptide is characterized by having:
- an amino-terminal domain of about 104 amino acids, wherein said amino-terminal domain has:
- (a) about 19% amino acid identity with the amino-terminal domain of hRXR-alpha,
- (b) about 24% amino acid identity with the amino-terminal domain of hGR, and
- (c) about 15% amino acid identity with the amino-terminal domain of hRAR.alpha.,
- a DNA binding domain of about 66 amino acids with 10 Cys residues, wherein said DNA binding domain has:
- (a) more than about 75% amino acid identity with the DNA binding domain of hRXR-alpha,
- (b) less than about 60% amino acid identity with the DNA binding domain of hGR, and
- (c) less than about 60% amino acid identity with the DNA binding domain of hRAR.alpha., and
- a ligand binding domain of about 290 amino acids, wherein said ligand binding domain has:
- (a) about 44% amino acid identity with the ligand binding domain of hRXR-alpha,
- (b) about 20% amino acid identity with the ligand binding domain of hGR, and
- (c) about 24% amino acid identity with the ligand binding domain of hRAR.alpha.,
- said method comprising:
- culturing cells which are transformed with an expression vector operable in said cells to express said receptor polypeptide.
- 2. A method according to claim 1 wherein the DNA binding domain of said receptor polypeptide has the same amino acid sequence as residues 104-169 shown in SEQ ID NO:1.
- 3. A method according to claim 2 wherein said receptor polypeptide has the same amino acid sequence as residues 1-513 shown in SEQ ID NO:1.
- 4. A method according to claim 3 wherein said receptor polypeptide is encoded by the same nucleotide sequence as nucleotides 163-1704 shown in SEQ ID NO:1.
- 5. An isolated receptor polypeptide which is structurally and functionally related to the steroid/thyroid superfamily of receptors, said receptor polypeptide characterized by having:
- an amino-terminal domain of about 104 amino acids, wherein said amino-terminal domain has:
- (a) about 19% amino acid identity with the amino-terminal domain of hRXR-alpha,
- (b) about 24% amino acid identity with the amino-terminal domain of hGR, and
- (c) about 15% amino acid identity with the amino-terminal domain of hRAR.alpha.,
- a DNA binding domain of about 66 amino acids with 10 Cys residues, wherein said DNA binding domain has:
- (a) more than about 75% amino acid identity with the DNA binding domain of hRXR-alpha,
- (b) less than about 60% amino acid identity with the DNA binding domain of hGR, and
- (c) less than about 60% amino acid identity with the DNA binding domain of hRAR.alpha., and
- a ligand binding domain of about 290 amino acids, wherein said ligand binding domain has:
- (a) about 44% amino acid identity with the ligand binding domain of hRXR-alpha,
- (b) about 20% amino acid identity with the ligand binding domain of hGR, and
- (c) about 24% amino acid identity with the ligand binding domain of hRAR.alpha..
- 6. A polypeptide according to claim 5 wherein the DNA binding domain of said polypeptide has the same amino acid sequence as residues 104-169 shown in SEQ ID NO:1.
- 7. A polypeptide according to claim 6 wherein said polypeptide has the same amino acid sequence as residues 1-513 shown in SEQ ID NO:1.
- 8. A polypeptide produced by the process of claim 1.
- 9. A method of making receptor polypeptide which is structurally and functionally related to the steroid/thyroid superfamily of receptors, wherein said receptor polypeptide is encoded by DNA obtained from a cDNA library, and wherein said DNA hybridizes under conditions of high stringency with the complementary strand of the DNA molecule having the sequence shown in SEQ ID NO:1, said method comprising:
- culturing cells which are transformed with an expression vector operable in said cells to express said receptor polypeptide.
- 10. An isolated receptor polypeptide which is structurally and functionally related to the steroid/thyroid superfamily of receptors, said receptor polypeptide being encoded by DNA obtained from a cDNA library, wherein said DNA hybridizes under conditions of high stringency with the complementary strand of the DNA molecule having the sequence shown in SEQ ID NO:1.
Parent Case Info
This application is a divisional application of U.S. Ser. No. 08/013,975, filed Feb. 4, 1993, now abandoned, which is a continuation-in-part application of U.S. Ser. No. 07/497,935, filed Mar. 22, 1990, now abandoned, the entire contents of which are hereby incorporated by reference herein.
US Referenced Citations (14)
Non-Patent Literature Citations (5)
Entry |
Heyman et al. (1992) Cell 68:397-406. |
Hamada et al. (1989) Proc. Nat. Acad. Aci. USA 86:8289-93. |
Wang et al. (1989) Nature 340:163-166. |
Henrich et al. (1990) Nucl. Acids Res. 18:4143-48. |
Mangelsdorf et al. (1990) Nature 345:224-229. |
Divisions (1)
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Number |
Date |
Country |
Parent |
13975 |
Feb 1993 |
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Continuation in Parts (1)
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Number |
Date |
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497935 |
Mar 1990 |
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