Claims
- 1. A dicot plant comprising in its genome a copy of a gene construction consisting essentially of, in sequence 5' to 3':
- a promoter sequence effective to initiate transcription in plant cells;
- a translational enhancer sequence from the transcribed but untranslated sequence immediately preceding the coding region of an alfalfa mosaic virus coat protein gene;
- a protein coding sequence for a protein of less than about 700 amino acids, the protein being a Bacillus thuringiensis delta-endotoxin which is toxic upon ingestion to Manduca sexta; and
- a polyadenylation sequence, the gene construction effectively expressing in the plant cells a protein toxic to Manduca sexta;
- wherein the plant is cotton.
- 2. Seed of the plant of claim 1.
- 3. A dicot plant comprising plant cells which are toxic to Manduca sexta upon ingestion, the plant cells comprising in their genome an expressed protein coding sequence consisting essentially of a DNA sequence coding for a Bacillus thuringiensis delta-endotoxin protein of less than about 700 amino acids which imbues the cells with lethal toxicity to Manduca sexta, the protein coding sequence immediately preceded by a translational enhancer sequence from the untranslated leader sequence of an alfalfa mosaic virus coat protein, wherein the plant is cotton.
- 4. Seed of the plant of claim 3.
- 5. A cotton plant comprising cells which are lethally toxic upon ingestion to Heliothis zea, the cotton cells comprising in their genome an expressed protein coding sequence consisting essentially of a DNA sequence coding for a protein of less than about 700 amino acids which is an amino-terminal portion of a Bacillus thuringiensis delta endotoxin which imbues the cells with lethal toxicity to Heliothis zea.
- 6. A cotton plant comprising cells which are lethally toxic upon ingestion by Heliothis zea, the cotton cells comprising in their genome an expressed protein coding sequence consisting essentially of a DNA sequence coding for a protein of the amino acid sequence set forth in FIG. 5.
- 7. Seed of the plant of claim 5.
CROSS-REFERENCE TO RELATED APPLICATION
This application is a continuation-in-part of application Ser. No. 123,054 filed Nov. 19, 1987, now abandoned, and a continuation-in-part of Ser. No. 937,384 filed Dec. 3, 1986, now U.S. Pat. No. 5,004,863.
US Referenced Citations (5)
Foreign Referenced Citations (4)
Number |
Date |
Country |
142924 |
May 1985 |
EPX |
178151 |
Apr 1986 |
EPX |
186379 |
Jul 1986 |
EPX |
0193259 |
Sep 1986 |
EPX |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
123054 |
Nov 1987 |
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