Integrated Top-Down/Bottom-Up Comprehensive Proteomics

Information

  • Research Project
  • 6785714
  • ApplicationId
    6785714
  • Core Project Number
    R44CA107988
  • Full Project Number
    1R44CA107988-01
  • Serial Number
    107988
  • FOA Number
    PAR-01-105
  • Sub Project Id
  • Project Start Date
    4/7/2004 - 20 years ago
  • Project End Date
    3/31/2005 - 19 years ago
  • Program Officer Name
    HEATH, ANNE K
  • Budget Start Date
    4/7/2004 - 20 years ago
  • Budget End Date
    3/31/2005 - 19 years ago
  • Fiscal Year
    2004
  • Support Year
    1
  • Suffix
  • Award Notice Date
    4/7/2004 - 20 years ago

Integrated Top-Down/Bottom-Up Comprehensive Proteomics

DESCRIPTION (provided by applicant): Phase I: The R43 phase of this project aims to develop and demonstrate a capillary gel electrophoresis (CGE) based multidimensional protein separation platform capable of providing significant analyte concentration and extremely high resolving power for handling complex protein mixtures prior to mass spectrometry detection. In comparison with conventional SDS-PAGE-based proteome approaches, the proposed CGE/capillary reversed-phase liquid chromatography (CRPLC) proteome technology will be highly automated and offer robust and high throughput fractionation of whole proteins for top-down proteomics while avoiding analyte dilution and loss in an integrated platform. By coupling with a nanoscale trypsin membrane reactor, the ultrafast proteolytic digestion of proteins resolved and eluted from the CGE-based separations enables the combined top-down/bottom-up characterization of post-translational modifications in complex proteomes. Research efforts in the R43 phase will address early implementation, optimization, and demonstration of the proposed proteome technology using yeast cell lysates.

IC Name
NATIONAL CANCER INSTITUTE
  • Activity
    R44
  • Administering IC
    CA
  • Application Type
    1
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
    175908
  • Sub Project Total Cost
  • ARRA Funded
  • CFDA Code
    396
  • Ed Inst. Type
  • Funding ICs
    NCI:175908\
  • Funding Mechanism
  • Study Section
    ZCA1
  • Study Section Name
    Special Emphasis Panel
  • Organization Name
    CALIBRANT BIOSYSTEMS, INC.
  • Organization Department
  • Organization DUNS
  • Organization City
    Gaithersburg
  • Organization State
    MD
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    20878
  • Organization District
    UNITED STATES