Claims
- 1. A method for detecting a member of a ligand pair comprising:
- contacting a target member of a ligand pair with a capture member of the ligand pair, said capture member immobilized on a solid support having intrinsic fluorescence, and said contacted pair in association with a colorimetric reporter;
- irradiating the solid support, said solid support attached to the ligand pair in association with the colorimetric reporter; and
- determining the resultant fluorescence.
- 2. The method according to claim 1 wherein the target member is an antibody and the capture member is an antigen, said antigen capable of binding with said antibody.
- 3. The method according to claim 1 wherein the target member is a first nucleic acid and the capture member is a second nucleic acid, said first nucleic acid and said second nucleic acid being complementary.
- 4. A method for nucleic acid detection comprising:
- contacting a target nucleic acid and a complementary capture nucleic acid probe, said capture probe immobilized on a solid support having intrinsic fluorescence, and said contacted target nucleic acid-capture probe complex in association with a colorimetric reporter;
- irradiating the solid support, said solid support attached to the hybridized capture probe-target nucleic acid complex; and
- determining the resultant fluorescence.
- 5. The method of claim 4 wherein the colorimetric reporter is attached to a signal nucleic acid probe, said signal probe complementary to the target nucleic acid.
- 6. The method of claim 5 wherein the solid support is selected from the group consisting of nylons, polyethylene, polyvinylchloride, polystyrene, polypropylene, acrylonitrile butadiene styrene, acrylics, acetals, polycarbonates, fluoroplastics, polyesters, phenolics, amino resins, epoxies, polyurethane, and silicones.
- 7. The method of claim 6 wherein the solid support is a nylon bead.
- 8. The method of claim 7 wherein the colorimetric reporter is an enzyme.
- 9. The method of claim 8 wherein the reporter enzyme is selected from the group consisting of horseradish peroxidase, alkaline phosphatase, and .beta.-galactosidase.
- 10. A method for detecting a member of a ligand pair comprising:
- immobilizing a capture member of a ligand pair on a composition comprising a solid support having its intrinsic fluorescence quenched;
- contacting the target member of a ligand pair with the immobilized capture member of the ligand pair, said contacted pair in association with a fluorescent reporter;
- irradiating the solid support, said solid support attached to the ligand pair in association with the fluorescent reporter; and
- determining the resultant fluorescence.
- 11. The method according to claim 10 wherein the target member is a first nucleic acid and the capture member is a second nucleic acid, said first nucleic acid and said second nucleic acid being complementary.
- 12. The method according to claim 11 wherein resultant fluorescence is determined with a fluorometer.
- 13. A method for quantitating the amount of fluorescent quenching product deposited on a solid support having intrinsic fluorescence, comprising:
- (a) depositing a colorimetric reporter as a quenching product on a solid support having intrinsic fluorescence;
- (b) irradiating the solid support having the colorimetric reporter with a wavelength of light that produces fluorescence of the solid support; and then
- (c) comparing fluorescence of the solid support having the colorimetric reporter with fluorescence from solid support lacking the colorimetric reporter, thereby quantitating the amount of deposited colorimetric reporter.
- 14. The method of claim 13, wherein the solid support is nylon.
- 15. The method of claim 13, wherein the solid support has a diameter of about 0.01 inch to about 0.5 inch.
- 16. The method of claim 13, wherein the colorimetric reporter is an insoluble enzymatic product.
- 17. The method of claim 13, wherein the wavelength is about 240 nm to about 750 nm.
- 18. The method of claim 13, further including the step of separating the solid support having the colorimetric reporter from colorimetric reporter not deposited on the solid support after step (a) and before step (b).
CROSS REFERENCE TO RELATED APPLICATIONS
This application is a continuation-in-part of U.S. Ser. No. 07/522,442, filed May 11, 1990 (assigned to the assignee of the present application and incorporated herein by reference).
US Referenced Citations (5)
Non-Patent Literature Citations (5)
Entry |
Dunn et al., Cell 12:23-26 (1977). |
Miller et al., Clin. Chem. 30:1467-1472 (1984). |
Brown et al., Clin. Chem. 31:1500-1505 (1985). |
Kobayashi et al., Steroids 36:177-183 (1980). |
Hemmila, Clin. Chem. 31:359-370 (1985). |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
522442 |
May 1990 |
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