Claims
- 1. An isolated nucleic acid, wherein the nucleic acid comprises a polynucleotide selected from the group consisting of:
a) a polynucleotide as defined in SEQ ID NO: 8, SEQ ID NO: 5, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, or SEQ ID NO: 16; b) a polynucleotide encoding a polypeptide as defined in SEQ ID NO: 6, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, or SEQ ID NO: 17; and c) a polynucleotide complementary to a polynucleotide of any of a) or b) above.
- 2. An isolated nucleic acid, wherein the nucleic acid comprises a polynucleotide encoding a polypeptide having at least 55% sequence identity with a polypeptide as defined in SEQ ID NO: 6, SEQ ID NO: 9, SEQ ID NO: 15, or SEQ ID NO: 17; and wherein the nucleic acid encodes a polypeptide that functions as a modulator of a plant stress response.
- 3. An isolated nucleic acid, wherein the nucleic acid comprises a polynucleotide encoding a polypeptide having at least 76% sequence identity with a polypeptide as defined in SEQ ID NO: 6, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, or SEQ ID NO: 17; and wherein the nucleic acid encodes a polypeptide that functions as a modulator of a plant stress response.
- 4. The nucleic acid of claim 2, wherein the nucleic acid comprises a polynucleotide encoding a polypeptide having at least 90% sequence identity with a polypeptide as defined in SEQ ID NO: 6, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, or SEQ ID NO: 17; and wherein the nucleic acid encodes a polypeptide that functions as a modulator of a plant stress response.
- 5. The nucleic acid of claim 1, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO: 6.
- 6. The nucleic acid of claim 1, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO: 9.
- 7. The nucleic acid of claim 1, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO: 11.
- 8. The nucleic acid of claim 1, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO: 13.
- 9. The nucleic acid of claim 1, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO: 15.
- 10. The nucleic acid of claim 1, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO: 17.
- 11. A vector comprising a nucleic acid of claim 1.
- 12. An isolated nucleic acid comprising a polynucleotide sequence encoding a polypeptide selected from the group consisting of an AKT-2 and a ZT-1 from Physcomitrella patens, an AKT-2 from Brassica napus, a ZT-1 and a ZT-2 from Glycine max, and a ZT-1 from Oryza sativa.
- 13. A first isolated nucleic acid that hybridizes under stringent conditions to at least one second nucleic acid selected from the group consisting of:
a) a second nucleic acid comprising a polynucleotide as defined in SEQ ID NO: 5, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, or SEQ ID NO: 16; and b) a second nucleic acid encoding a polypeptide as defined in SEQ ID NO: 6, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, or SEQ ID NO: 17.
- 14. A transgenic plant cell comprising a nucleic acid of any of claims 1, 2, 3, 4, 12, or 13.
- 15. A transgenic plant comprising a plant cell of claim 14.
- 16. The plant of claim 15, wherein the plant is a monocot.
- 17. The plant of claim 15, wherein the plant is a dicot.
- 18. The plant of claim 15, wherein the plant is selected from the group consisting of maize, wheat, rye, oat, triticale, rice, barley, soybean, peanut, cotton, rapeseed, canola, manihot, pepper, sunflower, tagetes, solanaceous plants, potato, tobacco, eggplant, tomato, Vicia species, pea, alfalfa, coffee, cacao, tea, Salix species, oil palm, coconut, perennial grass, and a forage crop.
- 19. The plant of claim 15, wherein the nucleic acid encodes a polypeptide that functions as a modulator of a plant stress response, and wherein the stress is selected from one or more of the group consisting of high salinity, drought, and low temperature.
- 20. A plant seed produced by the plant of claim 15, wherein the plant seed comprises the nucleic acid.
- 21. The seed of claim 20, wherein the seed is true breeding for an increased tolerance to environmental stress as compared to a wild type variety of the seed.
- 22. A method of producing a transgenic plant containing a nucleic acid wherein the plant has an increased tolerance to an environmental stress as compared to a wild type variety of the plant comprising, transforming a plant cell with an expression vector comprising the nucleic acid and generating from the plant cell the transgenic plant, wherein the nucleic acid encodes a polypeptide that functions as a modulator of a plant stress response, and wherein the nucleic acid is selected from the group consisting of:
a) a polynucleotide as defined in SEQ ID NO: 5, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, or SEQ ID NO: 16; b) a polynucleotide encoding a polypeptide as defined in SEQ ID NO: 6, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, or SEQ ID NO: 17; c) a polynucleotide encoding a polypeptide having at least 55% sequence identity with the polypeptide as defined in SEQ ID NO: 6, SEQ ID NO: 9, SEQ ID NO: 15, or SEQ ID NO: 17; d) a polynucleotide encoding a polypeptide having at least 76% sequence identity with the polypeptide as defined in SEQ ID NO: 11 or SEQ ID NO: 13; e) a polynucleotide comprising at least 60 consecutive nucleotides of any of a) or b) above; and f) a polynucleotide complementary to a polynucleotide of any of a) through e) above.
- 23. The method of claim 22, wherein the environmental stress is selected from one or more of the group consisting of high salinity, drought, and low temperature.
- 24. The method of claim 22, wherein the plant is a monocot.
- 25. The method of claim 22, wherein the plant is a dicot.
- 26. The method of claim 22, wherein the plant is selected from the group consisting of maize, wheat, rye, oat, triticale, rice, barley, soybean, peanut, cotton, rapeseed, canola, manihot, pepper, sunflower, tagetes, solanaceous plants, potato, tobacco, eggplant, tomato, Vicia species, pea, alfalfa, coffee, cacao, tea, Salix species, oil palm, coconut, perennial grass, and a forage crop.
- 27. The method of claim 22, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide as defined in SEQ ID NO: 6.
- 28. The method of claim 22, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide as defined in SEQ ID NO: 9.
- 29. The method of claim 22, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide as defined in SEQ ID NO: 11.
- 30. The method of claim 22, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide as defined in SEQ ID NO: 13.
- 31. The method of claim 22, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide as defined in SEQ ID NO: 15.
- 32. The method of claim 22, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide as defined in SEQ ID NO: 17.
- 33. The method of claim 22, wherein the nucleic acid comprises a polynucleotide encoding a polypeptide having at least 55% sequence identity to the polypeptide as defined in SEQ ID NO: 6, SEQ ID NO: 9, SEQ ID NO: 15, or SEQ ID NO: 17.
- 34. The method of claim 22, wherein the nucleic acid comprises a polynucleotide encoding a polypeptide having at least 76% sequence identity to the polypeptide as defined in SEQ ID NO: 6, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, or SEQ ID NO: 17.
- 35. The method of claim 22, wherein the nucleic acid comprises a polynucleotide encoding a polypeptide having at least 90% sequence identity to the polypeptide as defined in SEQ ID NO: 6, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, or SEQ ID NO: 17.
- 36. The method of claim 22, wherein the nucleic acid comprises at least 60 consecutive nucleotides of any of the polynucleotides of a) or b) of claim 22.
- 37. The method of claim 22, wherein the plant's stress tolerance is increased by increasing expression of the nucleic acid in the plant.
- 38. The method of claim 22, wherein the nucleic acid encodes a polypeptide that functions as an ion transprorter.
- 39. The method of claim 22, wherein the plant is transformed with a promoter that directs expression of the nucleic acid, and wherein said promoter is selected from one or more of the group consisting of tissue-preferred, developmentally regulated, and stress-inducible.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] The present invention claims the priority benefit of U.S. Provisional Patent Application Serial No. 60/345,816 filed Dec. 31, 2001, the entire contents of which are hereby incorporated by reference.
Provisional Applications (1)
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Number |
Date |
Country |
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60345816 |
Dec 2001 |
US |