Claims
- 1. An isolated nucleic acid sequence comprising SEQ ID NO: 1.
- 2. An isolated nucleic acid sequence encoding SEQ ID NO: 2.
- 3. A purified protein comprising sequence SEQ ID NO: 2.
- 4. A recombinant DNA molecule comprising the nucleic acid sequence of claim 1 operably linked to at least one suitable regulatory sequence.
- 5. A recombinant DNA molecule comprising the nucleic acid sequence of claim 2 operably linked to at least one suitable regulatory sequence.
- 6. An expression vector comprising the recombinant DNA molecule of claim 4.
- 7. An organism transformed with the recombinant DNA molecule of claim 4.
- 8. The organism of claim 7, wherein the organism is selected from the group consisting of microorganisms and plants.
- 9. A plant transformed with the recombinant DNA molecule of claim 4.
- 10. The transgenic plant of claim 9, wherein the plant is selected from the group consisting of soybean (Glycine max), rapeseed and canola (including Brassica napus and B. campestris), sunflower (Helianthus annus), cotton (Gossypium hirsutum), corn (Zea mays), cocoa (Theobroma cacao), safflower (Carthamus tinctorius), oil palm (Elaeis guineensis), coconut palm (Cocos nucifera), flax (Linum usitatissimum), castor (Ricinus communis) and peanut (Arachis hypogaea).
- 11. A plant cell transformed with the recombinant DNA molecule of claim 4.
- 12. A plant seed transformed with the recombinant DNA molecule of claim 4.
- 13. An expression vector comprising the recombinant DNA molecule of claim 5.
- 14. An organism transformed with the recombinant DNA molecule of claim 5.
- 15. The organism of claim 14, wherein the organism is selected from the group consisting of microorganisms and plants.
- 16. A plant transformed with the recombinant DNA molecule of claim 5.
- 17. The transgenic plant of claim 16, wherein the plant is selected from the group consisting of soybean (Glycine max), rapeseed and canola (including Brassica napus and B. campestris), sunflower (Helianthus annus), cotton (Gossypium hirsutum), corn (Zea mays), cocoa (Theobroma cacao), safflower (Carthamus tinctorius), oil palm (Elaeis guineensis), coconut palm (Cocos nucifera), flax (Linum usitatissimum), castor (Ricinus communis) and peanut (Arachis hypogaea).
- 18. A plant cell transformed with the recombinant DNA molecule of claim 5.
- 19. A plant seed transformed with the recombinant DNA molecule of claim 5.
- 20. A method of producing a plant hydroxylase in a transgenic organism, comprising:
a) providing an organism transformed with the recombinant DNA molecule of claim 4;b) growing the organism under conditions such that a hydroxylase encoded by SEQ ID NO: 1 is expressed.
- 21. The method of claim 20, wherein the organism is a plant.
- 22. The method of claim 21, wherein the recombinant DNA molecule is integrated into the genome of the plant.
- 23. A plant which produces a plant hydroxylase according to claim 20.
- 24. A method of producing a plant hydroxylase in a transgenic organism, comprising:
a) providing an organism transformed with the recombinant DNA molecule of claim 5;b) growing the organism under conditions such that a hydroxylase encoded by SEQ ID NO: 1 is expressed.
- 25. The method of claim 24, wherein the organism is a plant.
- 26. The method of claim 25, wherein the recombinant DNA molecule is integrated into the genome of the plant.
- 27. A plant which produces a plant hydroxylase according to claim 24.
- 28. A method for altering the phenotype of a plant comprising:
a) providing:
i) a vector comprising a nucleic acid sequence encoding a protein, where the nucleic acid sequence is selected from the group consisting of SEQ ID NO: 1 and a nucleic acid sequence which encodes SEQ ID NO: 2; ii) a plant or a plant tissue or a plant cell; b) transfecting the plant or plant tissue or plant cell with the vector under conditions such that the protein is expressed in a plant obtained from the plant or plant tissue or plant cell.
- 29. A method for altering the phenotype of a plant comprising:
a) providing:
i) a vector comprising an antisense sequence, corresponding to a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 and a nucleic acid sequence which encodes SEQ ID NO: 2; ii) a plant or a plant tissue or a plant cell; b) transfecting said plant or plant tissue or plant cell with said vector under conditions such that said antisense sequence is expressed in a plant obtained from the plant or plant tissue or plant cell and wherein the activity of an oleate hydroxylase is down-regulated as compared to wild-type.
Parent Case Info
[0001] This application claims priority from provisional application Serial No. 60/302,801, filed Jun. 29, 2001, which is hereby incorporated by reference in its entirety.
Government Interests
[0002] This invention was made at least in part with Government support under contract number DE-AC02-98CH10886, awarded by the U.S. Department of Energy. The Government may have certain rights in the invention.
Provisional Applications (1)
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Number |
Date |
Country |
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60302801 |
Jun 2001 |
US |