Isolated polynucleotides expressing or modulating microRNAs or targets of same, transgenic plants comprising same and uses thereof

SEQUENCE LISTING STATEMENT

The ASCII file, entitled 59933SequenceListing.txt, created on Jul. 17, 2014, comprising 18,690,790 bytes, submitted concurrently with the filing of this application is incorporated herein by reference.

FIELD AND BACKGROUND OF THE INVENTION

The present invention, in some embodiments thereof, relates to isolated polynucleotides expressing or modulating microRNAs or targets of same, transgenic plants comprising same and uses thereof in improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of a plant.

Consumption of soybean for food production is increasing worldwide because of its reported beneficial health effects. Soybean is also viewed as an attractive crop for the production of biodiesel. Importantly, it has the ability to fix atmospheric nitrogen, which in turn may cut the input of nitrogen fertilizer that often accounts for the single largest energy input in agriculture.

With a growing world population, increasing demand for food, fuel and fiber, and a changing climate, agriculture faces unprecedented challenges. In general, shortage in water supply is one of the most severe global agricultural problems affecting plant growth and crop yield. Excessive efforts are made to alleviate the harmful effects of desertification of the world's arable land. Farmers are seeking advanced, biotechnology-based solutions to enable them to obtain stable high yields and give them the potential to reduce irrigation costs or to grow crops in areas where potable water is a limiting factor. It should be noted that improved abiotic stress (ABST) tolerance will confer plants with improved vigor also under non-stress conditions, resulting in crops having improved biomass and/or yield.

ABST is a collective term for numerous extreme environmental parameters such as drought, high or low salinity, high or low temperature/light, and nutrient imbalances. The major agricultural crops (corn, rice, wheat, canola and soybean) account for over half of total human caloric intake, giving their overall yield and quality vast importance. ABST causes more than 50% yield loss of the above mentioned major crops. Among the various ABSTs, drought is the major factor that limits crop productivity worldwide. Short-term conditions of reduced environmental water content typically occur during the life cycle of most crop plants. Although most plants have evolved strategies to survive these conditions, when the severity and duration of drought become too great, major alterations to the plant metabolism take place. As a result, the plant development, growth and yield profoundly diminish. Furthermore, drought is associated with increased susceptibility to various diseases. ABST-induced dehydration or osmotic stress, in the form of reduced availability of water and disruption of turgor pressure, cause irreversible cellular damage. A water-limiting environment at various plant developmental stages may activate various physiological changes.

In soybean, drought, for instance, reduces yield by approximately 40%, with the most critical period for water deprivation being the flowering stage and the period following flowering. Water deficit, salinity and low/high temperatures are stresses that cause plant cellular dehydration, due to transpiration rate that exceeds water uptake. Water use efficiency (WUE), defined as the amount of biomass accumulated per unit of water used, plays an important role in determining a plant's ability to tolerate drought stress. The higher the WUE of a plant, the higher the crop productivity and total biomass yield under drought conditions. Thus, efforts are made worldwide to increase the WUE of the most important crops and reach the best yield performance under extreme water deficiency conditions.

Studies have shown that plant adaptations to drought and other adverse environmental conditions are complex genetic traits with polygenic nature. Conventional means for crop and horticultural improvements utilize selective breeding techniques to identify plants having desirable characteristics. However, selective breeding is tedious, time consuming and has an unpredictable outcome. Furthermore, limited germplasm resources for yield improvement and incompatibility in crosses between distantly related plant species represent significant problems encountered in conventional breeding. Advances in genetic engineering have allowed mankind to modify the germplasm of plants by expression of genes-of-interest in plants. Such a technology has the capacity to generate crops or plants with improved economic, agronomic or horticultural traits. However, generation of transgenic plants expressing full-length genes is typically hampered by the selection of optimal regulatory sequences and identification of those rare transformation events that exhibit sufficient levels of gene products expression.

SUMMARY OF THE INVENTION

According to an aspect of some embodiments of the present invention there is provided a method of improving abiotic stress tolerance, nitrogen use efficiency, biomass, vigor or yield of a plant, the method comprising expressing within the plant an exogenous polynucleotide which downregulates an activity or expression of a gene encoding an RNAi molecule having a nucleic acid sequence at least 90% identical to SEQ ID NOs: 139, 57-79, 202-219, 126-138, 140-161, 236-255, 169-173, 260-261, 3953-5114, 5117-6277, 6278, 11905-11909, 11940-11955, 11959-11961, wherein the RNAi molecule regulates abiotic stress tolerance of the plant, thereby improving abiotic stress tolerance, nitrogen use efficiency, biomass, vigor or yield of the plant.

According to an aspect of some embodiments of the present invention there is provided a method of improving abiotic stress tolerance, nitrogen use efficiency, biomass, vigor or yield of a plant, the method comprising expressing within the plant an exogenous polynucleotide having a nucleic acid sequence at least 90% identical to SEQ ID NOs: 1-56, 174-201, 80-125, 220-235, 162-168, 256-259, 262-2086, 2087-3910, 3911, 11616, 11615, 11874, 11875-11904, 11910-11939, 11956, 11957 or 11958, wherein the nucleic acid sequence is capable of regulating abiotic stress tolerance of the plant, thereby improving abiotic stress tolerance, nitrogen use efficiency, biomass, vigor or yield of the plant.

According to an aspect of some embodiments of the present invention there is provided a transgenic plant exogenously expressing a polynucleotide having a nucleic acid sequence at least 90% identical to SEQ ID NOs: SEQ ID NOs: 1-56, 174-201, 80-125, 220-235, 162-168, 256-259, 262-2086, 2087-3910, 3911, 11616, 11615, 11874, 11875-11904, 11910-11939, 11956, 11957 or 11958, wherein the nucleic acid sequence is capable of regulating abiotic stress tolerance of the plant.

According to some embodiments of the invention, the exogenous polynucleotide encodes a precursor of the nucleic acid sequence.

According to some embodiments of the invention, the precursor of the nucleic acid sequence is at least 60% identical to SEQ ID NO: 174-201, 220-235, 256-259, 2087-3910, 3911, 11910-11939, 11615, 11956, 11957 or 11958.

According to some embodiments of the invention, the precursor of the nucleic acid sequence is at least 60% identical to SEQ ID NO: 174-201, 220-235, 256-259, 2087-3910, 3911, 11875-11904, 11910-11939, 11615, 11956, 11957 or 11958.

According to some embodiments of the invention, the exogenous polynucleotide encodes a mature miRNA.

According to some embodiments of the invention, the exogenous polynucleotide is selected from the group consisting of SEQ ID NO: 1-56, 174-201, 80-125, 220-235, 162-168, 256-259, 262-2086, 2087-3910, 3911, 11616, 11615, 11874, 11875-11904, 11910-11939, 11956, 11957 or 11958.

According to an aspect of some embodiments of the present invention there is provided a nucleic acid construct comprising a nucleic acid sequence being at least 90% identical to SEQ ID NO: 139, 1-201, 202-235, 236-3910, 3911, 11616, 11615, 11874, 11875-11904, 11910-11939, 11956, 11957, 11958, 11940-11955, 11905-11909, 11959-11961, wherein the nucleic acid sequence is capable of regulating abiotic stress tolerance of a plant and wherein the nucleic acid sequence is under the regulation of a cis-acting regulatory element.

According to some embodiments of the invention, the nucleic acid sequence is selected from the group consisting of SEQ ID NOs: 1-56, 174-201, 80-125, 220-235, 162-168, 256-259, 262-2086, 2087-3910, 3911, 11616, 11615, 11874, 11875-11904, 11910-11939, 11956, 11957 or 11958.

According to some embodiments of the invention, the nucleic acid sequence is selected from the group consisting of SEQ ID NOs: 139, 57-79, 202-219, 126-138, 140-161, 236-255, 169-173, 260-261, 3953-5114, 5117-6277, 6278, 11905-11909, 11959-11961, 11940-11955.

According to an aspect of some embodiments of the present invention there is provided a transgenic plant exogenously expressing a polynucleotide which downregulates an activity or expression of a gene encoding an RNAi molecule having a nucleic acid sequence at least 90% identical to SEQ ID NOs: 139, 57-79, 202-219, 126-138, 140-161, 236-255, 169-173, 260-261, 3953-5114, 5117-6277, 6278 11905-11909, 11940-11955, 11959-11961.

According to an aspect of some embodiments of the present invention there is provided a transgenic plant exogenously expressing a polynucleotide which downregulates an activity or expression of a gene encoding an RNAi molecule having a nucleic acid sequence at least 90% identical to SEQ ID NOs: 139, 1-201, 202-235, 236-3910, 3911, 11616, 11615, 11874, 11875-11904, 11910-11939, 11956, 11957, 11958, 11905-11909, 11940-11955, 11959-11961.

According to an aspect of some embodiments of the present invention there is provided an isolated polynucleotide which downregulates an activity or expression of a gene encoding an RNAi molecule having a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 139, 57-79, 202-219, 126-138, 140-161, 236-255, 169-173, 260-261, 3953-5114, 5117-6277, 6278, 11905-11909, 11940-11955, 11959-11961.

According to an aspect of some embodiments of the present invention there is provided an isolated polynucleotide which downregulates an activity or expression of a gene encoding an RNAi molecule having a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 139, 1-201, 202-235, 236-3910, 3911, 3953-5114, 5117-6277, 6278, 11616, 11615, 11874, 11875-11904, 11910-11939, 11956, 11957, 11958, 11905-11909, 11940-11955, 11959-11961.

According to an aspect of some embodiments of the present invention there is provided an isolated polynucleotide which downregulates an activity or expression of a gene encoding an RNAi molecule having a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 139, 1-201, 202-235, 236-3910, 3911, 11616, 11615, 11874, 11875-11904, 11910-11939, 11956, 11957, 11958, 11905-11909, 11940-11955, 11959-11961.

According to some embodiments of the invention, the polynucleotide encodes a miRNA-Resistant Target as set forth in SEQ ID NO: 11258-11359.

According to some embodiments of the invention, the polynucleotide encodes a miRNA-Resistant Target as set forth in SEQ ID NO: 11091-11257.

According to some embodiments of the invention, the isolated polynucleotide encodes a target mimic as set forth in SEQ ID NO: 11564-11613.

According to some embodiments of the invention, the polynucleotide encodes a target mimic as set forth in SEQ ID NO: 11437-11513.

According to an aspect of some embodiments of the present invention there is provided a nucleic acid construct comprising the isolated polynucleotide under the regulation of a cis-acting regulatory element.

According to some embodiments of the invention, the abiotic stress is selected from the group consisting of salinity, drought, water deprivation, flood, etiolation, low temperature, high temperature, heavy metal toxicity, anaerobiosis, nutrient deficiency, nutrient excess, atmospheric pollution and UV irradiation.

According to an aspect of some embodiments of the present invention there is provided a method of improving abiotic stress tolerance, nitrogen use efficiency, biomass, vigor or yield of a plant, the method comprising expressing within the plant an exogenous polynucleotide encoding a polypeptide having an amino acid sequence at least 80% homologous or identical to SEQ ID NOs: 9591-10364, wherein the polypeptide is capable of regulating abiotic stress tolerance of the plant, thereby improving abiotic stress tolerance, nitrogen use efficiency, biomass, vigor or yield of the plant.

According to an aspect of some embodiments of the present invention there is provided a transgenic plant exogenously expressing a polynucleotide encoding a polypeptide having an amino acid sequence at least 80% homologous or identical to SEQ ID NOs: 9591-10364, wherein the polypeptide is capable of regulating abiotic stress tolerance of the plant.

According to an aspect of some embodiments of the present invention there is provided a nucleic acid construct comprising a polynucleotide encoding a polypeptide having an amino acid sequence at least 80% homologous to SEQ ID NOs: 9591-10364, wherein the polypeptide is capable of regulating abiotic stress tolerance of the plant, and wherein the polynucleotide is under a transcriptional control of a cis-acting regulatory element.

According to some embodiments of the invention, the polynucleotide is selected from the group consisting of SEQ ID NO: 10365-10963.

According to some embodiments of the invention, the polypeptide is selected from the group consisting of SEQ ID NO: 9591-10364.

According to some embodiments of the invention, the cis-acting regulatory element comprises a promoter.

According to some embodiments of the invention, the promoter comprises a tissue-specific promoter.

According to some embodiments of the invention, the tissue-specific promoter comprises a root specific promoter.

According to some embodiments of the invention, the method further comprises growing the plant under limiting nitrogen conditions.

According to some embodiments of the invention, the method further comprises growing the plant under abiotic stress.

According to some embodiments of the invention, the plant is a monocotyledon.

According to some embodiments of the invention, the plant is a dicotyledon.

According to an aspect of some embodiments of the present invention there is provided a method of improving abiotic stress tolerance, nitrogen use efficiency, biomass, vigor or yield of a plant, the method comprising expressing within the plant an exogenous polynucleotide which downregulates an activity or expression of a polypeptide having an amino acid sequence at least 80% homologous or identical to SEQ ID NOs: 6315-8129, wherein the polypeptide is capable of regulating abiotic stress tolerance of the plant, thereby improving abiotic stress tolerance, nitrogen use efficiency, biomass, vigor or yield of the plant.

According to an aspect of some embodiments of the present invention there is provided a transgenic plant exogenously expressing a polynucleotide which downregulates an activity or expression of a polypeptide having an amino acid sequence at least 80% homologous or identical to SEQ ID NOs: 6315-8129, wherein the polypeptide is capable of regulating abiotic stress tolerance of the plant.

According to an aspect of some embodiments of the present invention there is provided a nucleic acid construct comprising a polynucleotide which downregulates an activity or expression of a polypeptide having an amino acid sequence at least 80% homologous to SEQ ID NOs: 6315-8129, wherein the polypeptide is capable of regulating abiotic stress tolerance of a plant, the nucleic acid sequence being under the regulation of a cis-acting regulatory element.

According to some embodiments of the invention, the polynucleotide acts by a mechanism selected from the group consisting of sense suppression, antisense suppression, ribozyme inhibition, gene disruption.

According to some embodiments of the invention, the cis-acting regulatory element comprises a promoter.

According to some embodiments of the invention, the promoter comprises a tissue-specific promoter.

According to some embodiments of the invention, the tissue-specific promoter comprises a root specific promoter.

Unless otherwise defined, all technical and/or scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the invention pertains. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of embodiments of the invention, exemplary methods and/or materials are described below. In case of conflict, the patent specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and are not intended to be necessarily limiting.

BRIEF DESCRIPTION OF THE DRAWINGS

Some embodiments of the invention are herein described, by way of example only, with reference to the accompanying drawings. With specific reference now to the drawings in detail, it is stressed that the particulars shown are by way of example and for purposes of illustrative discussion of embodiments of the invention. In this regard, the description taken with the drawings makes apparent to those skilled in the art how embodiments of the invention may be practiced.

In the drawings:

FIG. 1 is a schematic illustration of a plasmid map of the binary vector pORE-E1 used for plant transformation according to some embodiments of the invention.

FIG. 2 is a schematic illustration of a plasmid map of the binary vector pORE-E2 used for plant transformation according to some embodiments of the invention.

FIG. 3 is a schematic illustration of a plasmid map of the binary vector pORE-E3 used for plant transformation according to some embodiments of the invention.

DESCRIPTION OF SPECIFIC EMBODIMENTS OF THE INVENTION

Before explaining at least one embodiment of the invention in detail, it is to be understood that the invention is not necessarily limited in its application to the details set forth in the following description or exemplified by the Examples. The invention is capable of other embodiments or of being practiced or carried out in various ways.

Environmental stresses represent the most limiting factors for agricultural productivity. Apart from biotic stress caused by plant pathogens, there are a number of abiotic stresses such as extremes in temperature, drought, salinity, heavy metals and radiation which all have detrimental effects on plant growth and yield. Abiotic stresses lead to dehydration or osmotic stress through reduced availability of water for vital cellular functions and maintenance of turgor pressure. Stomata closure, reduced supply of CO₂and slower rate of biochemical reactions during prolonged periods of dehydration, high light intensity, high and low temperatures lead to high production of Reactive Oxygen Intermediates (ROI) in the chloroplasts causing irreversible cellular damage and photo inhibition.

Understanding the molecular mechanism for providing protection against biotic and abiotic stresses may lead to the identification of genes associated with stress tolerance. Optimum homeostasis is always a key to living organisms for adjusted environments.

While reducing the present invention to practice, the present inventors have uncovered dsRNA sequences that are differentially expressed in soy plants grown under abiotic stress conditions including, salt stress, heat stress and drought, versus soy plants grown under optimal conditions (see Example 1 of the Examples section which follows). Following extensive experimentation and screening, the present inventors have uncovered miRNA sequences that are upregulated or downregulated in leaf samples, and suggest using same or sequences controlling same in the generation of transgenic plants having improved abiotic stress tolerance.

Each of the above mechanisms may affect water uptake as well as salt absorption and therefore embodiments of the invention further relate to enhancement of abiotic stress tolerance, nitrogen use efficiency, biomass, vigor or yield of the plant.

Example 5 of the Examples section below, validates the present results by showing that some miRs (e.g., gma-miR4376-5p, zma-miR396b-3p, aly-miR396b-3p, gma-miR156g, ma-miRf10687-akr-omolog gma-miR159d, aly-miR396b-3p, gma-miR4416a, aly-miR396a-3p, zma-miR396b-3p, gma-miR4412-3p, csi-miR162-5p, ath-miRf10279-akr) according to specific embodiment of the invention are indeed differentially expressed under abiotic stress conditions as was initially identified by microarray analysis. The present inventors were also capable of generating transgenic plants which overexpress the indicated miRs (see Example 7).

Thus, according to an aspect of the invention there is provided a method of improving abiotic stress tolerance, nitrogen use efficiency, biomass, vigor or yield of a plant, the method comprising expressing within the plant an exogenous polynucleotide having a nucleic acid sequence at least 80%, 85%, 90% or 95% identical to SEQ ID NOs: 1-56, 174-201, 80-125, 220-235, 162-168, 256-259, 262-2086, 11616, 11615, 2087-3910, 3911, 11910-11939, 11874-11904, 11956, 11957 or 11958 wherein the nucleic acid sequence is capable of regulating abiotic stress tolerance of the plant, thereby improving abiotic stress tolerance, nitrogen use efficiency, biomass, vigor or yield of the plant.

According to a specific embodiment the exogenous polynucleotide has a nucleic acid sequence at least 90% identical to SEQ ID NOs: 1-56, 174-201, 80-125, 220-235, 162-168, 256-259, 262-2086, 11616, 11615, 2087-3910, 3911, 11910-11939, 11874-11904, 11956, 11957 or 11958.

According to a specific embodiment the exogenous polynucleotide has a nucleic acid sequence at least 95% identical to SEQ ID NOs: 1-56, 174-201, 80-125, 220-235, 162-168, 256-259, 262-2086, 11616, 11615, 2087-3910, 3911, 11910-11939, 11874-11904, 11956, 11957 or 11958.

According to a specific embodiment the exogenous polynucleotide has a nucleic acid sequence as set forth in SEQ ID NOs: 1-56, 174-201, 80-125, 220-235, 162-168, 256-259, 262-2086, 11616, 11615, 2087-3910, 3911, 11910-11939, 11874-11904, 11956, 11957 or 11958.

The phrase “abiotic stress” as used herein refers to any adverse effect on metabolism, growth, viability and/or reproduction of a plant. Abiotic stress can be induced by any of suboptimal environmental growth conditions such as, for example, water deficit or drought, flooding, freezing, low or high temperature, strong winds, heavy metal toxicity, anaerobiosis, high or low nutrient levels (e.g. nutrient deficiency), high or low salt levels (e.g. salinity), atmospheric pollution, high or low light intensities (e.g. insufficient light) or UV irradiation. Abiotic stress may be a short term effect (e.g. acute effect, e.g. lasting for about a week) or alternatively may be persistent (e.g. chronic effect, e.g. lasting for example 10 days or more). The present invention contemplates situations in which there is a single abiotic stress condition or alternatively situations in which two or more abiotic stresses occur.

According to an exemplary embodiment the abiotic stress refers to salinity.

According to another exemplary embodiment the abiotic stress refers to drought.

According to another exemplary embodiment the abiotic stress refers to a temperature stress.

As used herein the phrase “abiotic stress tolerance” refers to the ability of a plant to endure an abiotic stress without exhibiting substantial physiological or physical damage (e.g. alteration in metabolism, growth, viability and/or reproducibility of the plant).

As used herein the phrase “nitrogen use efficiency (NUE)” refers to a measure of crop production per unit of nitrogen fertilizer input. Fertilizer use efficiency (FUE) is a measure of NUE. Crop production can be measured by biomass, vigor or yield. The plant's nitrogen use efficiency is typically a result of an alteration in at least one of the uptake, spread, absorbance, accumulation, relocation (within the plant) and use of nitrogen absorbed by the plant. Improved NUE is with respect to that of a non-transgenic plant (i.e., lacking the transgene of the transgenic plant) of the same species and of the same developmental stage and grown under the same conditions.

As used herein the phrase “nitrogen-limiting conditions” refers to growth conditions which include a level (e.g., concentration) of nitrogen (e.g., ammonium or nitrate) applied which is below the level needed for optimal plant metabolism, growth, reproduction and/or viability.

As used herein the term/phrase “biomass”, “biomass of a plant” or “plant biomass” refers to the amount (e.g., measured in grams of air-dry tissue) of a tissue produced from the plant in a growing season. An increase in plant biomass can be in the whole plant or in parts thereof such as aboveground (e.g. harvestable) parts, vegetative biomass, roots and/or seeds or contents thereof (e.g., oil, starch etc.).

As used herein the term/phrase “vigor”, “vigor of a plant” or “plant vigor” refers to the amount (e.g., measured by weight) of tissue produced by the plant in a given time. Increased vigor could determine or affect the plant yield or the yield per growing time or growing area. In addition, early vigor (e.g. seed and/or seedling) results in improved field stand.

As used herein the term/phrase “yield”, “yield of a plant” or “plant yield” refers to the amount (e.g., as determined by weight or size) or quantity (e.g., numbers) of tissues or organs produced per plant or per growing season. Increased yield of a plant can affect the economic benefit one can obtain from the plant in a certain growing area and/or growing time.

According to an exemplary embodiment the yield is measured by cellulose content, oil content, starch content and the like.

According to another exemplary embodiment the yield is measured by oil content.

According to another exemplary embodiment the yield is measured by protein content.

According to another exemplary embodiment, the yield is measured by seed number per plant or part thereof (e.g., kernel, bean).

A plant yield can be affected by various parameters including, but not limited to, plant biomass; plant vigor; plant growth rate; seed yield; seed or grain quantity; seed or grain quality; oil yield; content of oil, starch and/or protein in harvested organs (e.g., seeds or vegetative parts of the plant); number of flowers (e.g. florets) per panicle (e.g. expressed as a ratio of number of filled seeds over number of primary panicles); harvest index; number of plants grown per area; number and size of harvested organs per plant and per area; number of plants per growing area (e.g. density); number of harvested organs in field; total leaf area; carbon assimilation and carbon partitioning (e.g. the distribution/allocation of carbon within the plant); resistance to shade; number of harvestable organs (e.g. seeds), seeds per pod, weight per seed; and modified architecture [such as increase stalk diameter, thickness or improvement of physical properties (e.g. elasticity)].

As used herein the term “improving” or “increasing” refers to at least about 2%, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90% or greater increase in NUE, in tolerance to abiotic stress, in yield, in biomass or in vigor of a plant, as compared to a native or wild-type plants [i.e., plants not genetically modified to express the biomolecules (polynucleotides) of the invention, e.g., a non-transformed plant of the same species or a transformed plant transformed with a control vector, either of which being of the same developmental stage and grown under the same growth conditions as the transformed plant].

Improved plant NUE is translated in the field into either harvesting similar quantities of yield, while implementing less fertilizers, or increased yields gained by implementing the same levels of fertilizers. Thus, improved NUE or FUE has a direct effect on plant yield in the field.

The term “plant” as used herein encompasses whole plants, ancestors and progeny of the plants and plant parts, including seeds, shoots, stems, roots (including tubers), and isolated plant cells, tissues and organs. The plant may be in any form including suspension cultures, embryos, meristematic regions, callus tissue, leaves, gametophytes, sporophytes, pollen, and microspores.

As used herein the phrase “plant cell” refers to plant cells which are derived and isolated from disintegrated plant cell tissue or plant cell cultures.

As used herein the phrase “plant cell culture” refers to any type of native (naturally occurring) plant cells, plant cell lines and genetically modified plant cells, which are not assembled to form a complete plant, such that at least one biological structure of a plant is not present. Optionally, the plant cell culture of this aspect of the present invention may comprise a particular type of a plant cell or a plurality of different types of plant cells. It should be noted that optionally plant cultures featuring a particular type of plant cell may be originally derived from a plurality of different types of such plant cells.

Any commercially or scientifically valuable plant is envisaged in accordance with some embodiments of the invention. Plants that are particularly useful in the methods of the invention include all plants which belong to the super family Viridiplantae, in particular monocotyledonous and dicotyledonous plants including a fodder or forage legume, ornamental plant, food crop, tree, or shrub selected from the list comprising Acacia spp., Acer spp., Actinidia spp., Aesculus spp., Agathis australis, Albizia amara, Alsophila tricolor, Andropogon spp., Arachis spp, Areca catechu, 30 Astelia fragrans, Astragalus cicer, Baikiaea plurijuga, Betula spp., Brassica spp., Bruguiera gymnorrhiza, Burkea africana, Butea frondosa, Cadaba farinosa, Calliandra spp, Camellia sinensis, Canna indica, Capsicum spp., Cassia spp., Centroema pubescens, Chacoomeles spp., Cinnamomum cassia, Coffea arabica, Colophospermum mopane, Coronillia varia, Cotoneaster serotina, Crataegus spp., Cucumis spp., Cupressus spp., Cyathea dealbata, Cydonia oblonga, Cryptomeria japonica, Cymbopogon spp., Cynthea dealbata, Cydonia oblonga, Dalbergia monetaria, Davallia divaricata, Desmodium spp., Dicksonia squarosa, Dibeteropogon amplectens, Dioclea spp, Dolichos spp., Dorycnium rectum, Echinochloa pyramidalis, Ehraffia spp., Eleusine coracana, Eragrestis spp., Erythrina spp., Eucalyptus spp., Euclea schimperi, Eulalia vi/losa, Pagopyrum spp., Feijoa sellowlana, Fragaria spp., Flemingia spp, Freycinetia banksli, Geranium thunbergii, GinAgo biloba, Glycine javanica, Gliricidia spp, Gossypium hirsutum, Grevillea spp., Guibourtia coleosperma, Hedysarum spp., Hemaffhia altissima, Heteropogon contoffus, Hordeum vulgare, Hyparrhenia rufa, Hypericum erectum, Hypeffhelia dissolute, Indigo incamata, Iris spp., Leptarrhena pyrolifolia, Lespediza spp., Lettuca spp., Leucaena leucocephala, Loudetia simplex, Lotonus bainesli, Lotus spp., Macrotyloma axillare, Malus spp., Manihot esculenta, Medicago saliva, Metasequoia glyptostroboides, Musa sapientum, Nicotianum spp., Onobrychis spp., Ornithopus spp., Oryza spp., Peltophorum africanum, Pennisetum spp., Persea gratissima, Petunia spp., Phaseolus spp., Phoenix canariensis, Phormium cookianum, Photinia spp., Picea glauca, Pinus spp., Pisum sativam, Podocarpus totara, Pogonarthria fleckii, Pogonaffhria squarrosa, Populus spp., Prosopis cineraria, Pseudotsuga menziesii, Pterolobium stellatum, Pyrus communis, Quercus spp., Rhaphiolepsis umbellata, Rhopalostylis sapida, Rhus natalensis, Ribes grossularia, Ribes spp., Robinia pseudoacacia, Rosa spp., Rubus spp., Salix spp., Schyzachyrium sanguineum, Sciadopitys vefficillata, Sequoia sempervirens, Sequoiadendron giganteum, Sorghum bicolor, Spinacia spp., Sporobolus fimbriatus, Stiburus alopecuroides, Stylosanthos humilis, Tadehagi spp, Taxodium distichum, Themeda triandra, Trifolium spp., Triticum spp., Tsuga heterophylla, Vaccinium spp., Vicia spp., Vitis vinifera, Watsonia pyramidata, Zantedeschia aethiopica, Zea mays, amaranth, artichoke, asparagus, broccoli, Brussels sprouts, cabbage, canola, carrot, cauliflower, celery, collard greens, flax, kale, lentil, oilseed rape, okra, onion, potato, rice, soybean, straw, sugar beet, sugar cane, sunflower, tomato, squash tea, maize, wheat, barley, rye, oat, peanut, pea, lentil and alfalfa, cotton, rapeseed, canola, pepper, sunflower, tobacco, eggplant, eucalyptus, a tree, an ornamental plant, a perennial grass and a forage crop. Alternatively algae and other non-Viridiplantae can be used for the methods of the present invention.

According to some embodiments of the invention, the plant used by the method of the invention is a crop plant including, but not limited to, cotton, Brassica vegetables, oilseed rape, sesame, olive tree, palm oil, banana, wheat, corn or maize, barley, alfalfa, peanuts, sunflowers, rice, oats, sugarcane, soybean, turf grasses, barley, rye, sorghum, sugar cane, chicory, lettuce, tomato, zucchini, bell pepper, eggplant, cucumber, melon, watermelon, beans, hibiscus, okra, apple, rose, strawberry, chili, garlic, pea, lentil, canola, mums, arabidopsis, broccoli, cabbage, beet, quinoa, spinach, squash, onion, leek, tobacco, potato, sugarbeet, papaya, pineapple, mango, Arabidopsis thaliana, and also plants used in horticulture, floriculture or forestry, such as, but not limited to, poplar, fir, eucalyptus, pine, an ornamental plant, a perennial grass and a forage crop, coniferous plants, moss, algae, as well as other plants listed in World Wide Web (dot) nationmaster (dot) com/encyclopedia/Plantae.

According to a specific embodiment of the present invention, the plant comprises soy.

As used herein, the phrase “exogenous polynucleotide” refers to a heterologous nucleic acid sequence which may not be naturally expressed within the plant or which overexpression [i.e., expression above that found in the control non-transformed plant (e.g., wild type) grown under the same conditions and being of the same developmental stage] in the plant is desired. The exogenous polynucleotide may be introduced into the plant in a stable or transient manner, so as to produce a ribonucleic acid (RNA) molecule. It should be noted that the exogenous polynucleotide may comprise a nucleic acid sequence which is identical or partially identical (homologous) to an endogenous nucleic acid sequence of the plant.

As mentioned, the present teachings are based on the identification of miRNA sequences which regulate the tolerance of plants to abiotic stress.

According to some embodiments the exogenous polynucleotide encodes a miRNA or a precursor thereof.

As used herein, the phrase “microRNA (also referred to herein interchangeably as “miRNA” or “miR”) or a precursor thereof” refers to a microRNA (miRNA) molecule acting as a post-transcriptional regulator. Typically, the miRNA molecules are RNA molecules of about 20 to 22 nucleotides in length which can be loaded into a RISC complex and which direct the cleavage of another RNA molecule, wherein the other RNA molecule comprises a nucleotide sequence essentially complementary to the nucleotide sequence of the miRNA molecule.

Typically, a miRNA molecule is processed from a “pre-miRNA” or as used herein a precursor of a pre-miRNA molecule by proteins, such as DCL proteins, present in any plant cell and loaded onto a RISC complex where it can guide the cleavage of the target RNA molecules.

Pre-microRNA molecules are typically processed from pri-microRNA molecules (primary transcripts). The single stranded RNA segments flanking the pre-microRNA are important for processing of the pri-miRNA into the pre-miRNA. The cleavage site appears to be determined by the distance from the stem-ssRNA junction (Han et al. 2006, Cell 125, 887-901, 887-901).

According to a specific embodiment, the miRNA and its complement are located about 10 to about 20 nucleotides from the free ends of the miRNA double stranded RNA stem. The length and sequence of the single stranded loop region are not critical and may vary considerably, e.g. between 30 and 50 nt in length. The complementarity between the miRNA and its complement need not be perfect and about 1 to 3 bulges of unpaired nucleotides can be tolerated. The secondary structure adopted by an RNA molecule can be predicted by computer algorithms conventional in the art such as mFOLD. The particular strand of the double stranded RNA stem from the pre-miRNA which is released by DCL activity and loaded onto the RISC complex is determined by the degree of complementarity at the 5′ end, whereby the strand which at its 5′ end is the least involved in hydrogen bounding between the nucleotides of the different strands of the cleaved dsRNA stem is loaded onto the RISC complex and will determine the sequence specificity of the target RNA molecule degradation. However, if empirically the miRNA molecule from a particular synthetic pre-miRNA molecule is not functional (because the “wrong” strand is loaded on the RISC complex), it will be immediately evident that this problem can be solved by exchanging the position of the miRNA molecule and its complement on the respective strands of the dsRNA stem of the pre-miRNA molecule. As is known in the art, binding between A and U involving two hydrogen bounds, or G and U involving two hydrogen bounds is less strong that between G and C involving three hydrogen bounds. Exemplary hairpin sequences are provided in Tables 1-8, below.

Naturally occurring miRNA molecules may be comprised within their naturally occurring pre-miRNA molecules but they can also be introduced into existing pre-miRNA molecule scaffolds by exchanging the nucleotide sequence of the miRNA molecule normally processed from such existing pre-miRNA molecule for the nucleotide sequence of another miRNA of interest. The scaffold of the pre-miRNA can also be completely synthetic. Likewise, synthetic miRNA molecules may be comprised within, and processed from, existing pre-miRNA molecule scaffolds or synthetic pre-miRNA scaffolds. Some pre-miRNA scaffolds may be preferred over others for their efficiency to be correctly processed into the designed microRNAs, particularly when expressed as a chimeric gene wherein other DNA regions, such as untranslated leader sequences or transcription termination and polyadenylation regions are incorporated in the primary transcript in addition to the pre-microRNA.

According to the present teachings, the miRNA molecules may be naturally occurring or synthetic.

Thus, the present teachings contemplate expressing an exogenous polynucleotide having a nucleic acid sequence at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% 99% or 100% identical to—NOs: 1-56, 80-125, 11874, 262-2086, 11616, (mature), provided that they regulate ABST.

Alternatively or additionally, the present teachings contemplate expressing an exogenous polynucleotide having a nucleic acid sequence at least 65%, 50%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% 99% or 100% identical to SEQ ID NOs: 1-56, 174-201, 80-125, 220-235, 162-168, 256-259, 262-2086, 11616, 11615, 2087-3910, 3911, 11910-11939, 11874-11904, 11956, 11957 or 11958 (mature and precursors Tables 1, 3, 5, 7), provided that they regulate abiotic stress tolerance of the plant.

Tables 1, 3, 5 and 7 below illustrate exemplary miRNA sequences and precursors thereof which over expression are associated with modulation of abiotic stress tolerance. It is noted that Tables 1-17 below are incorporated into the specification and are considered an integral part thereof.

The present invention envisages the use of homologous and orthologous sequences of the above miRNA molecules. At the precursor level use of homologous sequences can be done to a much broader extent. Thus, in such precursor sequences the degree of homology may be lower in all those sequences not including the mature miRNA segment therein.

As used herein, the phrase “stem-loop precursor” refers to stem loop precursor RNA structure from which the miRNA can be processed.

As used herein, a “pre-miRNA” molecule is an RNA molecule of about 100 to about 200 nucleotides, preferably about 100 to about 130 nucleotides which can adopt a secondary structure comprising a double stranded RNA stem and a single stranded RNA loop (also referred to as “hairpin”) and further comprising the nucleotide sequence of the miRNA (and its complement sequence) in the double stranded RNA stem. According to a specific embodiment, the miRNA and its complement are located about 10 to about 20 nucleotides from the free ends of the miRNA double stranded RNA stem. The length and sequence of the single stranded loop region are not critical and may vary considerably, e.g. between 30 and 50 nt in length. The complementarity between the miRNA and its complement need not be perfect and about 1 to 3 bulges of unpaired nucleotides can be tolerated. The secondary structure adopted by an RNA molecule can be predicted by computer algorithms conventional in the art such as mFOLD. The particular strand of the double stranded RNA stem from the pre-miRNA which is released by DCL activity and loaded onto the RISC complex is determined by the degree of complementarity at the 5′ end, whereby the strand which at its 5′ end is the least involved in hydrogen bounding between the nucleotides of the different strands of the cleaved dsRNA stem is loaded onto the RISC complex and will determine the sequence specificity of the target RNA molecule degradation. However, if empirically the miRNA molecule from a particular synthetic pre-miRNA molecule is not functional (because the “wrong” strand is loaded on the RISC complex), it will be immediately evident that this problem can be solved by exchanging the position of the miRNA molecule and its complement on the respective strands of the dsRNA stem of the pre-miRNA molecule. As is known in the art, binding between A and U involving two hydrogen bounds, or G and U involving two hydrogen bounds is less strong that between G and C involving three hydrogen bounds.

Thus, according to a specific embodiment, the exogenous polynucleotide encodes a stem-loop precursor of the nucleic acid sequence. Such a stem-loop precursor can be at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95% or more identical to SEQ ID NOs: SEQ ID NO: 174-201, 220-235, 256-259, 2087-3910, 3911, 11910-11939, 11615, 11875-11904, 11956, 11957 or 11958 (homologs precursor), provided that it regulates abiotic stress tolerance.

Identity (e.g., percent identity) can be determined using any homology comparison software, including for example, the BlastN software of the National Center of Biotechnology Information (NCBI) such as by using default parameters.

Homology (e.g., percent homology, identity+similarity) can be determined using any homology comparison software, including for example, the TBLASTN software of the National Center of Biotechnology Information (NCBI) such as by using default parameters.

According to some embodiments of the invention, the term “homology” or “homologous” refers to identity of two or more nucleic acid sequences; or identity of two or more amino acid sequences.

Homologous sequences include both orthologous and paralogous sequences. The term “paralogous” relates to gene-duplications within the genome of a species leading to paralogous genes. The term “orthologous” relates to homologous genes in different organisms due to ancestral relationship.

One option to identify orthologues in monocot plant species is by performing a reciprocal blast search. This may be done by a first blast involving blasting the sequence-of-interest against any sequence database, such as the publicly available NCBI database which may be found at: Hypertext Transfer Protocol://World Wide Web (dot) ncbi (dot) nlm (dot) nih (dot) gov. The blast results may be filtered. The full-length sequences of either the filtered results or the non-filtered results are then blasted back (second blast) against the sequences of the organism from which the sequence-of-interest is derived. The results of the first and second blasts are then compared. An orthologue is identified when the sequence resulting in the highest score (best hit) in the first blast identifies in the second blast the query sequence (the original sequence-of-interest) as the best hit. Using the same rational a paralogue (homolog to a gene in the same organism) is found. In case of large sequence families, the ClustalW program may be used [Hypertext Transfer Protocol://World Wide Web (dot) ebi (dot) ac (dot) uk/Tools/clustalw2/index (dot) html], followed by a neighbor joining tree (Hypertext Transfer Protocol://en (dot) wikipedia (dot) org/wiki/Neighbor-joining) which helps visualizing the clustering.

As mentioned, the present inventors have also identified RNAi sequences which are down regulated under abiotic stress conditions.

Thus, according to an aspect of the invention there is provided a method of improving abiotic stress tolerance, nitrogen use efficiency, biomass, vigor or yield of a plant, the method comprising expressing within the plant an exogenous polynucleotide which downregulates an activity or expression of a gene encoding a miRNA molecule having a nucleic acid sequence at least 80%, 85% or preferably 90%, 95% or even 100% identical to the sequence selected from the group consisting of SEQ ID NOs: 57-79, 202-219, 126-161, 236-255, 169-173, 260-261, 3953-5114, 11905-11909, 11940-11955, 11959-11961, 5117-6277 or 6278 (Tables 2, 4, 6 and 8), thereby improving abiotic stress tolerance, nitrogen use efficiency, biomass, vigor or yield of a plant.

There are various approaches to down regulate miRNA sequences.

As used herein the term “down-regulation” refers to reduced activity or expression of the miRNA (at least 10%, 20%, 30%, 50%, 60%, 70%, 80%, 90% or 100% reduction in activity or expression) as compared to its activity or expression in a plant of the same species and the same developmental stage not expressing the exogenous polynucleotide.

Nucleic acid agents that down-regulate miR activity include, but are not limited to, a target mimic, a micro-RNA resistant gene and a miRNA inhibitor.

The target mimic or micro-RNA resistant target is essentially complementary to the microRNA provided that one or more of following mismatches are allowed:

(a) a mismatch between the nucleotide at the 5′ end of the microRNA and the corresponding nucleotide sequence in the target mimic or micro-RNA resistant target;

(b) a mismatch between any one of the nucleotides in position 1 to position 9 of the microRNA and the corresponding nucleotide sequence in the target mimic or micro-RNA resistant target; or

(c) three mismatches between any one of the nucleotides in position 12 to position 21 of the microRNA and the corresponding nucleotide sequence in the target mimic or micro-RNA resistant target provided that there are no more than two consecutive mismatches.

The target mimic RNA is essentially similar to the target RNA modified to render it resistant to miRNA induced cleavage, e.g. by modifying the sequence thereof such that a variation is introduced in the nucleotide of the target mimic sequence complementary to the nucleotides 10 or 11 of the miRNA resulting in a mismatch.

Alternatively, a microRNA-resistant target may be implemented. Thus, a silent mutation may be introduced in the microRNA binding site of the target gene so that the DNA and resulting RNA sequences are changed in a way that prevents microRNA binding, but the amino acid sequence of the protein is unchanged. Thus, a new sequence can be synthesized instead of the existing binding site, in which the DNA sequence is changed, resulting in lack of miRNA binding to its target.

Tables 14-17 below provide non-limiting examples of target mimics and target resistant sequences that can be used to down-regulate the activity of the miRs of the invention. According to a specific embodiment, the target mimic is listed in any one of the sequences of Table 7. According to a specific embodiment, the mir-resistant target sequence is listed in any one of the sequences of Table 15.

According to a specific embodiment, the target mimic or micro-RNA resistant target is linked to the promoter naturally associated with the pre-miRNA recognizing the target gene and introduced into the plant cell. In this way, the miRNA target mimic or micro-RNA resistant target RNA will be expressed under the same circumstances as the miRNA and the target mimic or micro-RNA resistant target RNA will substitute for the non-target mimic/micro-RNA resistant target RNA degraded by the miRNA induced cleavage.

Non-functional miRNA alleles or miRNA resistant target genes may also be introduced by homologous recombination to substitute the miRNA encoding alleles or miRNA sensitive target genes.

Recombinant expression is effected by cloning the nucleic acid of interest (e.g., miRNA, target gene, silencing agent etc) into a nucleic acid expression construct under the expression of a plant promoter, as further described hereinbelow.

In other embodiments of the invention, synthetic single stranded nucleic acids are used as miRNA inhibitors. A miRNA inhibitor is typically between about 17 to 25 nucleotides in length and comprises a 5′ to 3′ sequence that is at least 90% complementary to the 5′ to 3′ sequence of a mature miRNA. In certain embodiments, a miRNA inhibitor molecule is 17, 18, 19, 20, 21, 22, 23, 24, or 25 nucleotides in length, or any range derivable therein. Moreover, a miRNA inhibitor has a sequence (from 5′ to 3′) that is or is at least 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 99.1, 99.2, 99.3, 99.4, 99.5, 99.6, 99.7, 99.8, 99.9 or 100% complementary, or any range derivable therein, to the 5′ to 3′ sequence of a mature miRNA, particularly a mature, naturally occurring miRNA.

While further reducing the present invention to practice, the present inventors have identified gene targets for the differentially expressed miRNA molecules. It is therefore contemplated, that gene targets of those miRNAs that are down regulated during stress should be overexpressed in order to confer tolerance, while gene targets of those miRNAs that are up regulated during stress should be downregulated in the plant in order to confer tolerance.

Thus, according to an aspect of the invention there is provided a method of improving abiotic stress tolerance, nitrogen use efficiency, biomass, vigor or yield of a plant, the method comprising expressing within the plant an exogenous polynucleotide encoding a polypeptide having an amino acid sequence at least 80%, 82%, 84%, 85%, 86%, 88%, 90%, 92%, 94%, 95%, 96%, 97%, 98%, 99% or 100% homologous to SEQ ID NOs: 9591-10364 (gene targets of down regulated miRNAs, see Table 10), wherein the polypeptide is capable of regulating abiotic stress tolerance of the plant, thereby improving abiotic stress tolerance, nitrogen use efficiency, biomass, vigor or yield of the plant.

Nucleic acid sequences (also referred to herein as polynucleotides) of the polypeptides of some embodiments of the invention may be optimized for expression in a specific plant host. Examples of such sequence modifications include, but are not limited to, an altered G/C content to more closely approach that typically found in the plant species of interest, and the removal of codons atypically found in the plant species commonly referred to as codon optimization.

The phrase “codon optimization” refers to the selection of appropriate DNA nucleotides for use within a structural gene or fragment thereof that approaches codon usage within the plant of interest. Therefore, an optimized gene or nucleic acid sequence refers to a gene in which the nucleotide sequence of a native or naturally occurring gene has been modified in order to utilize statistically-preferred or statistically-favored codons within the plant. The nucleotide sequence typically is examined at the DNA level and the coding region optimized for expression in the plant species determined using any suitable procedure, for example as described in Sardana et al. (1996, Plant Cell Reports 15:677-681). In this method, the standard deviation of codon usage, a measure of codon usage bias, may be calculated by first finding the squared proportional deviation of usage of each codon of the native gene relative to that of highly expressed plant genes, followed by a calculation of the average squared deviation. The formula used is: 1 SDCU=n=1 N [(Xn−Yn)/Yn]2/N, where Xn refers to the frequency of usage of codon n in highly expressed plant genes, where Yn to the frequency of usage of codon n in the gene of interest and N refers to the total number of codons in the gene of interest. A table of codon usage from highly expressed genes of dicotyledonous plants is compiled using the data of Murray et al. (1989, Nuc Acids Res. 17:477-498).

One method of optimizing the nucleic acid sequence in accordance with the preferred codon usage for a particular plant cell type is based on the direct use, without performing any extra statistical calculations, of codon optimization tables such as those provided on-line at the Codon Usage Database through the NIAS (National Institute of Agrobiological Sciences) DNA bank in Japan (www(dot)kazusa(dot)or(dot)jp/codon/). The Codon Usage Database contains codon usage tables for a number of different species, with each codon usage table having been statistically determined based on the data present in Genbank.

By using the above tables to determine the most preferred or most favored codons for each amino acid in a particular species (for example, rice), a naturally-occurring nucleotide sequence encoding a protein of interest can be codon optimized for that particular plant species. This is effected by replacing codons that may have a low statistical incidence in the particular species genome with corresponding codons, in regard to an amino acid, that are statistically more favored. However, one or more less-favored codons may be selected to delete existing restriction sites, to create new ones at potentially useful junctions (5′ and 3′ ends to add signal peptide or termination cassettes, internal sites that might be used to cut and splice segments together to produce a correct full-length sequence), or to eliminate nucleotide sequences that may negatively effect mRNA stability or expression.

The naturally-occurring encoding nucleotide sequence may already, in advance of any modification, contain a number of codons that correspond to a statistically-favored codon in a particular plant species. Therefore, codon optimization of the native nucleotide sequence may comprise determining which codons, within the native nucleotide sequence, are not statistically-favored with regards to a particular plant, and modifying these codons in accordance with a codon usage table of the particular plant to produce a codon optimized derivative. A modified nucleotide sequence may be fully or partially optimized for plant codon usage provided that the protein encoded by the modified nucleotide sequence is produced at a level higher than the protein encoded by the corresponding naturally occurring or native gene. Construction of synthetic genes by altering the codon usage is described in for example PCT Patent Application 93/07278.

Target genes which are contemplated according to the present teachings are provided in the polynucleotide sequences which comprise nucleic acid sequences as set forth in the soy polynucleotides listed in Table 10). However the present teachings also relate to orthologs or homologs at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, or at least about 95% or more identical or similar to SEQ ID NO: 10365-10963 (polynucleotides listed in Table 10). Parameters for determining the level of identity are provided hereinbelow.

Alternatively or additionally, target genes which are contemplated according to the present teachings are provided in the polypeptide sequences which comprise amino acid sequences as set forth in the soy polypeptides of Table 10). However the present teachings also relate to orthologs or homologs at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, or at least about 95% or more identical or similar to SEQ ID NO: 9591-10364 (Table 10).

As mentioned the present inventors have also identified genes which down-regulation may be done in order to improve their abiotic stress tolerance, NUE, biomass, vigor and yield.

Thus, according to an aspect of the invention there is provided a method of improving abiotic stress tolerance, nitrogen use efficiency, biomass, vigor or yield of a plant, the method comprising expressing within the plant an exogenous polynucleotide which downregulates an activity or expression of a polypeptide having an amino acid sequence at least 80%, 85%, 90%, 95%, or 100% homologous to SEQ ID NOs: 6315-8129 (polypeptides of Table 9), wherein the polypeptide is capable of regulating abiotic stress tolerance of the plant, thereby improving abiotic stress tolerance, nitrogen use efficiency, biomass, vigor or yield of the plant.

Down regulation of activity or expression is by at least 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% or even complete (100%) loss of activity or expression. Assays for measuring gene expression can be effected at the protein level (e.g., Western blot, ELISA) or at the mRNA level such as by RT-PCR.

According to a specific embodiment the amino acid sequence of the target gene is as set forth in SEQ ID NOs: 6315-8129 of Table 9.

Alternatively or additionally, the amino acid sequence of the target gene is encoded by a polynucleotide sequence as set forth in SEQ ID NOs: 8130-9590 of Table 9.

Examples of polynucleotide downregulating agents that inhibit (also referred to herein as inhibitors or nucleic acid (downregulating) agents) the expression of a target gene are given below.

1. Polynucleotide-Based Inhibition of Gene Expression.

It will be appreciated, that any of these methods when specifically referring to downregulating expression/activity of the target genes can be used, at least in part, to downregulate expression or activity of endogenous RNA molecules.

i. Sense Suppression/Cosuppression

In some embodiments of the invention, inhibition of the expression of target gene may be obtained by sense suppression or cosuppression. For cosuppression, an expression cassette is designed to express an RNA molecule corresponding to all or part of a messenger RNA encoding a target gene in the “sense” orientation. Over-expression of the RNA molecule can result in reduced expression of the native gene. Accordingly, multiple plant lines transformed with the cosuppression expression cassette are screened to identify those that show the greatest inhibition of target gene expression.

The polynucleotide used for cosuppression may correspond to all or part of the sequence encoding the target gene, all or part of the 5′ and/or 3′ untranslated region of a target transcript, or all or part of both the coding sequence and the untranslated regions of a transcript encoding the target gene. In some embodiments where the polynucleotide comprises all or part of the coding region for the target gene, the expression cassette is designed to eliminate the start codon of the polynucleotide so that no protein product will be transcribed.

Cosuppression may be used to inhibit the expression of plant genes to produce plants having undetectable protein levels for the proteins encoded by these genes. See, for example, Broin, et al., (2002) Plant Cell 15:1517-1532. Cosuppression may also be used to inhibit the expression of multiple proteins in the same plant. Methods for using cosuppression to inhibit the expression of endogenous genes in plants are described in Flavell, et al., (1995) Proc. Natl. Acad. Sci. USA 91:3590-3596; Jorgensen, et al., (1996) Plant Mol. Biol. 31:957-973; Johansen and Carrington, (2001) Plant Physiol. 126:930-938; Broin, et al., (2002) Plant Cell 15:1517-1532; Stoutjesdijk, et al., (2002) Plant Physiol. 129:1723-1731; Yu, et al., (2003) Phytochemistry 63:753-763; and U.S. Pat. Nos. 5,035,323, 5,283,185 and 5,952,657; each of which is herein incorporated by reference. The efficiency of cosuppression may be increased by including a poly-dt region in the expression cassette at a position 3′ to the sense sequence and 5′ of the polyadenylation signal. See, US Patent Publication Number 20020058815, herein incorporated by reference. Typically, such a nucleotide sequence has substantial sequence identity to the sequence of the transcript of the endogenous gene, optimally greater than about 65% sequence identity, more optimally greater than about 85% sequence identity, most optimally greater than about 95% sequence identity. See, U.S. Pat. Nos. 5,283,185 and 5,035,323; herein incorporated by reference.

Transcriptional gene silencing (TGS) may be accomplished through use of hpRNA constructs wherein the inverted repeat of the hairpin shares sequence identity with the promoter region of a gene to be silenced. Processing of the hpRNA into short RNAs which can interact with the homologous promoter region may trigger degradation or methylation to result in silencing. (Aufsatz, et al., (2002) PNAS 99(4):16499-16506; Mette, et al., (2000) EMBO J. 19(19):5194-5201)

ii. Antisense Suppression

In some embodiments of the invention, inhibition of the expression of the target gene may be obtained by antisense suppression. For antisense suppression, the expression cassette is designed to express an RNA molecule complementary to all or part of a messenger RNA encoding the target gene. Over-expression of the antisense RNA molecule can result in reduced expression of the native gene. Accordingly, multiple plant lines transformed with the antisense suppression expression cassette are screened to identify those that show the greatest inhibition of target gene expression.

The polynucleotide for use in antisense suppression may correspond to all or part of the complement of the sequence encoding the target gene, all or part of the complement of the 5′ and/or 3′ untranslated region of the target gene transcript, or all or part of the complement of both the coding sequence and the untranslated regions of a transcript encoding the target gene. In addition, the antisense polynucleotide may be fully complementary (i.e., 100% identical to the complement of the target sequence) or partially complementary (i.e., less than 100% identical to the complement of the target sequence) to the target sequence. Antisense suppression may be used to inhibit the expression of multiple proteins in the same plant. Furthermore, portions of the antisense nucleotides may be used to disrupt the expression of the target gene. Generally, sequences of at least 50 nucleotides, 100 nucleotides, 200 nucleotides, 300, 500, 550 or greater may be used. Methods for using antisense suppression to inhibit the expression of endogenous genes in plants are described, for example, in Liu, et al., (2002) Plant Physiol. 129:1732-1753 and U.S. Pat. No. 5,759,829, which is herein incorporated by reference. Efficiency of antisense suppression may be increased by including a poly-dT region in the expression cassette at a position 3′ to the antisense sequence and 5′ of the polyadenylation signal. See, US Patent Publication Number 20020058815.

iii. Double-Stranded RNA Interference

In some embodiments of the invention, inhibition of the expression of a target gene may be obtained by double-stranded RNA (dsRNA) interference. For dsRNA interference, a sense RNA molecule like that described above for cosuppression and an antisense RNA molecule that is fully or partially complementary to the sense RNA molecule are expressed in the same cell, resulting in inhibition of the expression of the corresponding endogenous messenger RNA.

Expression of the sense and antisense molecules can be accomplished by designing the expression cassette to comprise both a sense sequence and an antisense sequence. Alternatively, separate expression cassettes may be used for the sense and antisense sequences. Multiple plant lines transformed with the dsRNA interference expression cassette or expression cassettes are then screened to identify plant lines that show the greatest inhibition of target gene expression. Methods for using dsRNA interference to inhibit the expression of endogenous plant genes are described in Waterhouse, et al., (1998) Proc. Natl. Acad. Sci. USA 95:13959-13965, Liu, et al., (2002) Plant Physiol. 129:1732-1753, and WO 99/59029, WO 99/53050, WO 99/61631, and WO 00/59035.

iv. Hairpin RNA Interference and Intron-Containing Hairpin RNA Interference

In some embodiments of the invention, inhibition of the expression of one or more target gene may be obtained by hairpin RNA (hpRNA) interference or intron-containing hairpin RNA (ihpRNA) interference. These methods are highly efficient at downregulating the expression of endogenous genes. See, Waterhouse and Helliwell, (2003) Nat. Rev. Genet. 5:29-38 and the references cited therein.

For hpRNA interference, the expression cassette is designed to express an RNA molecule that hybridizes with itself to form a hairpin structure that comprises a single-stranded loop region and a base-paired stem. The base-paired stem region comprises a sense sequence corresponding to all or part of the endogenous messenger RNA encoding the gene whose expression is to be inhibited, and an antisense sequence that is fully or partially complementary to the sense sequence. Thus, the base-paired stem region of the molecule generally determines the specificity of the RNA interference. hpRNA molecules are highly efficient at inhibiting the expression of endogenous genes, and the RNA interference they induce is inherited by subsequent generations of plants. See, for example, Chuang and Meyerowitz, (2000) Proc. Natl. Acad. Sci. USA 97:5985-5990; Stoutjesdijk, et al., (2002) Plant Physiol. 129:1723-1731; and Waterhouse and Helliwell, (2003) Nat. Rev. Genet. 5:29-38. Methods for using hpRNA interference to inhibit or silence the expression of genes are described, for example, in Chuang and Meyerowitz, (2000) Proc. Natl. Acad. Sci. USA 97:5985-5990; Stoutjesdijk, et al., (2002) Plant Physiol. 129:1723-1731; Waterhouse and Helliwell, (2003) Nat. Rev. Genet. 5:29-38; Pandolfini, et al., BMC Biotechnology 3:7, and US Patent Publication Number 20030175965; each of which is herein incorporated by reference. A transient assay for the efficiency of hpRNA constructs to silence gene expression in vivo has been described by Panstruga, et al., (2003) Mol. Biol. Rep. 30:135-150, herein incorporated by reference.

For ihpRNA, the interfering molecules have the same general structure as for hpRNA, but the RNA molecule additionally comprises an intron that is capable of being spliced in the cell in which the ihpRNA is expressed. The use of an intron minimizes the size of the loop in the hairpin RNA molecule following splicing, and this increases the efficiency of interference. See, for example, Smith, et al., (2000) Nature 507:319-320. In fact, Smith, et al., show 100% suppression of endogenous gene expression using ihpRNA-mediated interference. Methods for using ihpRNA interference to inhibit the expression of endogenous plant genes are described, for example, in Smith, et al., (2000) Nature 507:319-320; Wesley, et al., (2001) Plant J. 27:584, 1-3, 590; Wang and Waterhouse, (2001) Curr. Opin. Plant Biol. 5:156-150; Waterhouse and Helliwell, (2003) Nat. Rev. Genet. 5:29-38; Helliwell and Waterhouse, (2003) Methods 30:289-295, and US Patent Publication Number 20030180955, each of which is herein incorporated by reference.

The expression cassette for hpRNA interference may also be designed such that the sense sequence and the antisense sequence do not correspond to an endogenous RNA. In this embodiment, the sense and antisense sequence flank a loop sequence that comprises a nucleotide sequence corresponding to all or part of the endogenous messenger RNA of the target gene. Thus, it is the loop region that determines the specificity of the RNA interference. See, for example, WO 02/00905, herein incorporated by reference.

v. Amplicon-Mediated Interference

Amplicon expression cassettes comprise a plant virus-derived sequence that contains all or part of the target gene but generally not all of the genes of the native virus. The viral sequences present in the transcription product of the expression cassette allow the transcription product to direct its own replication. The transcripts produced by the amplicon may be either sense or antisense relative to the target sequence (i.e., the messenger RNA for target gene). Methods of using amplicons to inhibit the expression of endogenous plant genes are described, for example, in Angell and Baulcombe, (1997) EMBO J. 16:3675-3685, Angell and Baulcombe, (1999) Plant J. 20:357-362, and U.S. Pat. No. 6,656,805, each of which is herein incorporated by reference.

vi. Ribozymes

In some embodiments, the polynucleotide expressed by the expression cassette of the invention is catalytic RNA or has ribozyme activity specific for the messenger RNA of target gene. Thus, the polynucleotide causes the degradation of the endogenous messenger RNA, resulting in reduced expression of the target gene. This method is described, for example, in U.S. Pat. No. 5,987,071, herein incorporated by reference.

2. Gene Disruption

In some embodiments of the present invention, the activity of a miRNA or a target gene is reduced or eliminated by disrupting the gene encoding the target polypeptide. The gene encoding the target polypeptide may be disrupted by any method known in the art. For example, in one embodiment, the gene is disrupted by transposon tagging. In another embodiment, the gene is disrupted by mutagenizing plants using random or targeted mutagenesis, and selecting for plants that have reduced response regulator activity.

Any of the nucleic acid agents described herein (for overexpression or downregulation of either the target gene or the miRNA) can be provided to the plant as naked RNA or expressed from a nucleic acid expression construct, where it is operably linked to a regulatory sequence.

According to a specific embodiment of the invention, there is provided a nucleic acid construct comprising a nucleic acid sequence encoding a nucleic acid agent (e.g., miRNA or a precursor thereof as described herein, gene target or silencing agent), the nucleic acid sequence being under a transcriptional control of a regulatory sequence such as a tissue specific promoter.

An exemplary nucleic acid construct which can be used for plant transformation include, the pORE E2 binary vector (FIG. 1) in which the relevant nucleic acid sequence is ligated under the transcriptional control of a promoter.

A coding nucleic acid sequence is “operably linked” or “transcriptionally linked to a regulatory sequence (e.g., promoter)” if the regulatory sequence is capable of exerting a regulatory effect on the coding sequence linked thereto. Thus, the regulatory sequence controls the transcription of the miRNA or precursor thereof, gene target or silencing agent.

The term “regulatory sequence”, as used herein, means any DNA, that is involved in driving transcription and controlling (i.e., regulating) the timing and level of transcription of a given DNA sequence, such as a DNA coding for a miRNA, precursor or inhibitor of same. For example, a 5′ regulatory region (or “promoter region”) is a DNA sequence located upstream (i.e., 5′) of a coding sequence and which comprises the promoter and the 5′-untranslated leader sequence. A 3′ regulatory region is a DNA sequence located downstream (i.e., 3′) of the coding sequence and which comprises suitable transcription termination (and/or regulation) signals, including one or more polyadenylation signals.

For the purpose of the invention, the promoter is a plant-expressible promoter. As used herein, the term “plant-expressible promoter” means a DNA sequence which is capable of controlling (initiating) transcription in a plant cell. This includes any promoter of plant origin, but also any promoter of non-plant origin which is capable of directing transcription in a plant cell, i.e., certain promoters of viral or bacterial origin. Thus, any suitable promoter sequence can be used by the nucleic acid construct of the present invention. According to some embodiments of the invention, the promoter is a constitutive promoter, a tissue-specific promoter or an inducible promoter (e.g. an abiotic stress-inducible promoter).

Suitable constitutive promoters include, for example, hydroperoxide lyase (HPL) promoter, CaMV 35S promoter (Odell et al, Nature 313:810-812, 1985); Arabidopsis At6669 promoter (see PCT Publication No. WO04081173A2); Arabidopsis new At6669 promoter; maize Ubi 1 (Christensen et al., Plant Sol. Biol. 18:675-689, 1992); rice actin (McElroy et al., Plant Cell 2:163-171, 1990); pEMU (Last et al, Theor. Appl. Genet. 81:584, 1-3, 588, 1991); CaMV 19S (Nilsson et al, Physiol. Plant 100:456-462, 1997); GOS2 (de Pater et al, Plant J Nov; 2(6):837-44, 1992); ubiquitin (Christensen et al, Plant MoI. Biol. 18: 675-689, 1992); Rice cyclophilin (Bucholz et al, Plant MoI Biol. 25(5):837-43, 1994); Maize H3 histone (Lepetit et al, MoI. Gen. Genet. 231:276-285, 1992); Actin 2 (An et al, Plant J. 10(1); 107-121, 1996) and Synthetic Super MAS (Ni et al., The Plant Journal 7: 661-76, 1995). Other constitutive promoters include those in U.S. Pat. Nos. 5,659,026, 5,608,149; 5,608,144; 5,604,121; 5,569,597: 5,466,785; 5,399,680; 5,268,463; and 5,608,142.

Suitable tissue-specific promoters include, but not limited to, leaf-specific promoters [such as described, for example, by Yamamoto et al., Plant J. 12:255-265, 1997; Kwon et al., Plant Physiol. 105:357-67, 1994; Yamamoto et al., Plant Cell Physiol. 35:773-778, 1994; Gotor et al., Plant J. 3:509-18, 1993; Orozco et al., Plant MoI. Biol. 23:1129-1138, 1993; and Matsuoka et al., Proc. Natl. Acad. Sci. USA 90:9586-9590, 1993], seed-preferred promoters [e.g., from seed specific genes (Simon, et al., Plant MoI. Biol. 5. 191, 1985; Scofield, et al., J. Biol. Chem. 262: 12202, 1987; Baszczynski, et al., Plant MoI. Biol. 14: 633, 1990), Brazil Nut albumin (Pearson′ et al., Plant MoI. Biol. 18: 235-245, 1992), legumin (Ellis, et al. Plant MoI. Biol. 10: 203-214, 1988), Glutelin (rice) (Takaiwa, et al., MoI. Gen. Genet. 208: 15-22, 1986; Takaiwa, et al., FEBS Letts. 221:43-47, 1987), Zein (Matzke et al., Plant MoI Biol, 143)323-32 1990), napA (Stalberg, et al., Planta 199: 515-519, 1996), Wheat SPA (Albanietal, Plant Cell, 9: 171-184, 1997), sunflower oleosin (Cummins, et al, Plant MoI. Biol. 19: 873-876, 1992)], endosperm specific promoters [e.g., wheat LMW and HMW, glutenin-1 (MoI Gen Genet 216:81-90, 1989; NAR 17:461-2), wheat a, b and g gliadins (EMBO3: 1409-15, 1984), Barley ltrl promoter, barley Bl, C, D hordein (Theor Appl Gen 98:1253-62, 1999; Plant J 4:343-55, 1993; MoI Gen Genet 250:750-60, 1996), Barley DOF (Mena et al., The Plant Journal, 116(1): 53-62, 1998), Biz2 (EP99106056.7), Synthetic promoter (Vicente-Carbajosa et al., Plant J. 13: 629-640, 1998), rice prolamin NRP33, rice-globulin GIb-I (Wu et al., Plant Cell Physiology 39(8) 885-889, 1998), rice alpha-globulin REB/OHP-1 (Nakase et al. Plant MoI. Biol. 33: 513-S22, 1997), rice ADP-glucose PP (Trans Res 6:157-68, 1997), maize ESR gene family (Plant J 12:235-46, 1997), sorghum gamma-kafirin (PMB 32:1029-35, 1996); e.g., the Napin promoter], embryo specific promoters [e.g., rice OSH1 (Sato et al, Proc. Natl. Acad. Sci. USA, 93: 8117-8122), KNOX (Postma-Haarsma et al, Plant MoI. Biol. 39:257-71, 1999), rice oleosin (Wu et at, J. Biochem., 123:386, 1998)], and flower-specific promoters [e.g., AtPRP4, chalene synthase (chsA) (Van der Meer, et al., Plant MoI. Biol. 15, 95-109, 1990), LAT52 (Twell et al., MoI. Gen Genet. 217:240-245; 1989), apetala-3]. Also contemplated are root-specific promoters such as the ROOTP promoter described in Vissenberg K, et al. Plant Cell Physiol. 2005 January; 46(1):192-200.

The nucleic acid construct of some embodiments of the invention can further include an appropriate selectable marker and/or an origin of replication.

The nucleic acid construct of some embodiments of the invention can be utilized to stably or transiently transform plant cells. In stable transformation, the exogenous polynucleotide is integrated into the plant genome and as such it represents a stable and inherited trait. In transient transformation, the exogenous polynucleotide is expressed by the cell transformed but it is not integrated into the genome and as such it represents a transient trait.

When naked RNA or DNA is introduced into a cell, the polynucleotides may be synthesized using any method known in the art, including either enzymatic syntheses or solid-phase syntheses. These are especially useful in the case of short polynucleotide sequences with or without modifications as explained above. Equipment and reagents for executing solid-phase synthesis are commercially available from, for example, Applied Biosystems. Any other means for such synthesis may also be employed; the actual synthesis of the oligonucleotides is well within the capabilities of one skilled in the art and can be accomplished via established methodologies as detailed in, for example: Sambrook, J. and Russell, D. W. (2001), “Molecular Cloning: A Laboratory Manual”; Ausubel, R. M. et al., eds. (1994, 1989), “Current Protocols in Molecular Biology,” Volumes I-III, John Wiley & Sons, Baltimore, Md.; Perbal, B. (1988), “A Practical Guide to Molecular Cloning,” John Wiley & Sons, New York; and Gait, M. J., ed. (1984), “Oligonucleotide Synthesis”; utilizing solid-phase chemistry, e.g. cyanoethyl phosphoramidite followed by deprotection, desalting, and purification by, for example, an automated trityl-on method or HPLC.

There are various methods of introducing foreign genes into both monocotyledonous and dicotyledonous plants (Potrykus, L, Annu. Rev. Plant. Physiol, Plant. MoI. Biol. (1991) 42:205-225; Shimamoto et al., Nature (1989) 338:274-276).

The principle methods of causing stable integration of exogenous DNA into plant genomic DNA include two main approaches:

(i) Agrobacterium-mediated gene transfer (e.g., T-DNA using Agrobacterium tumefaciens or Agrobacterium rhizogenes); see for example, Klee et al. (1987) Annu. Rev. Plant Physiol. 38:467-486; Klee and Rogers in Cell Culture and Somatic Cell Genetics of Plants, Vol. 6, Molecular Biology of Plant Nuclear Genes, eds. Schell, J., and Vasil, L. K., Academic Publishers, San Diego, Calif. (1989) p. 2-25; Gatenby, in Plant Biotechnology, eds. Kung, S, and Arntzen, C. J., Butterworth Publishers, Boston, Mass. (1989) p. 93-112.

(ii) Direct DNA uptake: Paszkowski et al., in Cell Culture and Somatic Cell Genetics of Plants, Vol. 6, Molecular Biology of Plant Nuclear Genes eds. Schell, J., and Vasil, L. K., Academic Publishers, San Diego, Calif. (1989) p. 52-68; including methods for direct uptake of DNA into protoplasts, Toriyama, K. et al. (1988) Bio/Technology 6:1072-1074. DNA uptake induced by brief electric shock of plant cells: Zhang et al. Plant Cell Rep. (1988) 7:379-384. Fromm et al. Nature (1986) 319:791-793. DNA injection into plant cells or tissues by particle bombardment, Klein et al. Bio/Technology (1988) 6:559-563; McCabe et al. Bio/Technology (1988) 6:923-926; Sanford, Physiol. Plant. (1990) 79:206-209; by the use of micropipette systems: Neuhaus et al., Theor. Appl. Genet. (1987) 75:30-36; Neuhaus and Spangenberg, Physiol. Plant. (1990) 79:213-217; glass fibers or silicon carbide whisker transformation of cell cultures, embryos or callus tissue, U.S. Pat. No. 5,464,765 or by the direct incubation of DNA with germinating pollen, DeWet et al. in Experimental Manipulation of Ovule Tissue, eds. Chapman, G. P. and Mantell, S. H. and Daniels, W. Longman, London, (1985) p. 197-209; and Ohta, Proc. Natl. Acad. Sci. USA (1986) 83:715-719.

The Agrobacterium system includes the use of plasmid vectors that contain defined DNA segments that integrate into the plant genomic DNA. Methods of inoculation of the plant tissue vary depending upon the plant species and the Agrobacterium delivery system. A widely used approach is the leaf disc procedure which can be performed with any tissue explant that provides a good source for initiation of whole plant differentiation. See, e.g., Horsch et al. in Plant Molecular Biology Manual A5, Kluwer Academic Publishers, Dordrecht (1988) p. 1-9. A supplementary approach employs the Agrobacterium delivery system in combination with vacuum infiltration. The Agrobacterium system is especially viable in the creation of transgenic dicotyledonous plants.

According to a specific embodiment of the present invention, the exogenous polynucleotide is introduced into the plant by infecting the plant with bacteria, such as using a floral dip transformation method (as described in further detail in Example 7, of the Examples section which follows).

There are various methods of direct DNA transfer into plant cells. In electroporation, the protoplasts are briefly exposed to a strong electric field. In microinjection, the DNA is mechanically injected directly into the cells using very small micropipettes. In microparticle bombardment, the DNA is adsorbed on microprojectiles such as magnesium sulfate crystals or tungsten particles, and the microprojectiles are physically accelerated into cells or plant tissues.

Following stable transformation plant propagation is exercised. The most common method of plant propagation is by seed. Regeneration by seed propagation, however, has the deficiency that due to heterozygosity there is a lack of uniformity in the crop, since seeds are produced by plants according to the genetic variances governed by Mendelian rules. Basically, each seed is genetically different and each will grow with its own specific traits. Therefore, it is preferred that the transformed plant be produced such that the regenerated plant has the identical traits and characteristics of the parent transgenic plant. For this reason it is preferred that the transformed plant be regenerated by micropropagation which provides a rapid, consistent reproduction of the transformed plants.

Micropropagation is a process of growing new generation plants from a single piece of tissue that has been excised from a selected parent plant or cultivar. The new generation plants which are produced are genetically identical to, and have all of the characteristics of, the original plant. Micropropagation allows mass production of quality plant material in a short period of time and offers a rapid multiplication of selected cultivars in the preservation of the characteristics of the original transgenic or transformed plant. The advantages of cloning plants are the speed of plant multiplication and the quality and uniformity of plants produced.

Micropropagation is a multi-stage procedure that requires alteration of culture medium or growth conditions between stages. Thus, the micropropagation process involves four basic stages: Stage one, initial tissue culturing; stage two, tissue culture multiplication; stage three, differentiation and plant formation; and stage four, greenhouse culturing and hardening. During stage one, initial tissue culturing, the tissue culture is established and certified contaminant-free. During stage two, the initial tissue culture is multiplied until a sufficient number of tissue samples are produced to meet production goals. During stage three, the tissue samples grown in stage two are divided and grown into individual plantlets. At stage four, the transformed plantlets are transferred to a greenhouse for hardening where the plants' tolerance to light is gradually increased so that it can be grown in the natural environment.

Although stable transformation is presently preferred, transient transformation of leaf cells, meristematic cells or the whole plant is also envisaged by the present invention.

Transient transformation can be effected by any of the direct DNA transfer methods described above or by viral infection using modified plant viruses.

Viruses that have been shown to be useful for the transformation of plant hosts include CaMV, Tobacco mosaic virus (TMV), brome mosaic virus (BMV) and Bean Common Mosaic Virus (BV or BCMV). Transformation of plants using plant viruses is described in U.S. Pat. No. 4,855,237 (bean golden mosaic virus; BGV), EP-A 67,553 (TMV), Japanese Published Application No. 63-14693 (TMV), EPA 194,809 (BV), EPA 278,667 (BV); and Gluzman, Y. et al., Communications in Molecular Biology: Viral Vectors, Cold Spring Harbor Laboratory, New York, pp. 172-189 (1988). Pseudovirus particles for use in expressing foreign DNA in many hosts, including plants are described in WO 87/06261. According to some embodiments of the invention, the virus used for transient transformations is avirulent and thus is incapable of causing severe symptoms such as reduced growth rate, mosaic, ring spots, leaf roll, yellowing, streaking, pox formation, tumor formation and pitting. A suitable avirulent virus may be a naturally occurring avirulent virus or an artificially attenuated virus. Virus attenuation may be effected by using methods well known in the art including, but not limited to, sub-lethal heating, chemical treatment or by directed mutagenesis techniques such as described, for example, by Kurihara and Watanabe (Molecular Plant Pathology 4:259-269, 2003), Galon et al. (1992), Atreya et al. (1992) and Huet et al. (1994).

Suitable virus strains can be obtained from available sources such as, for example, the American Type culture Collection (ATCC) or by isolation from infected plants. Isolation of viruses from infected plant tissues can be effected by techniques well known in the art such as described, for example by Foster and Tatlor, Eds. “Plant Virology Protocols: From Virus Isolation to Transgenic Resistance (Methods in Molecular Biology (Humana Pr), VoI 81)”, Humana Press, 1998. Briefly, tissues of an infected plant believed to contain a high concentration of a suitable virus, preferably young leaves and flower petals, are ground in a buffer solution (e.g., phosphate buffer solution) to produce a virus infected sap which can be used in subsequent inoculations.

Construction of plant RNA viruses for the introduction and expression of non-viral exogenous polynucleotide sequences in plants is demonstrated by the above references as well as by Dawson, W. O. et al, Virology (1989) 172:285-292; Takamatsu et al. EMBO J. (1987) 6:307-311; French et al. Science (1986) 231:1294-1297; Takamatsu et al. FEBS Letters (1990) 269:73-76; and U.S. Pat. No. 5,316,931.

When the virus is a DNA virus, suitable modifications can be made to the virus itself. Alternatively, the virus can first be cloned into a bacterial plasmid for ease of constructing the desired viral vector with the foreign DNA. The virus can then be excised from the plasmid. If the virus is a DNA virus, a bacterial origin of replication can be attached to the viral DNA, which is then replicated by the bacteria. Transcription and translation of this DNA will produce the coat proteins which will encapsidate the viral DNA. If the virus is an RNA virus, the virus is generally cloned as a cDNA and inserted into a plasmid. The plasmid is then used to make all of the constructions. The RNA virus is then produced by transcribing the viral sequence of the plasmid and translation of the viral genes to produce the coat protein(s) which encapsidate the viral RNA.

In one embodiment, a plant viral nucleic acid is provided in which the native coat protein coding sequence has been deleted from a viral nucleic acid, a non-native plant viral coat protein coding sequence and a non-native promoter, preferably the subgenomic promoter of the non-native coat protein coding sequence, capable of expression in the plant host, packaging of the recombinant plant viral nucleic acid, and ensuring a systemic infection of the host by the recombinant plant viral nucleic acid, has been inserted. Alternatively, the coat protein gene may be inactivated by insertion of the non-native nucleic acid sequence within it, such that a protein is produced. The recombinant plant viral nucleic acid may contain one or more additional non-native subgenomic promoters. Each non-native subgenomic promoter is capable of transcribing or expressing adjacent genes or nucleic acid sequences in the plant host and incapable of recombination with each other and with native subgenomic promoters. Non-native (foreign) nucleic acid sequences may be inserted adjacent the native plant viral subgenomic promoter or the native and a non-native plant viral subgenomic promoters if more than one nucleic acid sequence is included. The non-native nucleic acid sequences are transcribed or expressed in the host plant under control of the subgenomic promoter to produce the desired products.

In a second embodiment, a recombinant plant viral nucleic acid is provided as in the first embodiment except that the native coat protein coding sequence is placed adjacent one of the non-native coat protein subgenomic promoters instead of a non-native coat protein coding sequence.

In a third embodiment, a recombinant plant viral nucleic acid is provided in which the native coat protein gene is adjacent its subgenomic promoter and one or more non-native subgenomic promoters have been inserted into the viral nucleic acid. The inserted non-native subgenomic promoters are capable of transcribing or expressing adjacent genes in a plant host and are incapable of recombination with each other and with native subgenomic promoters. Non-native nucleic acid sequences may be inserted adjacent to the non-native subgenomic plant viral promoters such that the sequences are transcribed or expressed in the host plant under control of the subgenomic promoters to produce the desired product.

In a fourth embodiment, a recombinant plant viral nucleic acid is provided as in the third embodiment except that the native coat protein coding sequence is replaced by a non-native coat protein coding sequence.

The viral vectors are encapsidated by the coat proteins encoded by the recombinant plant viral nucleic acid to produce a recombinant plant virus. The recombinant plant viral nucleic acid or recombinant plant virus is used to infect appropriate host plants. The recombinant plant viral nucleic acid is capable of replication in the host, systemic spread in the host, and transcription or expression of foreign gene(s) (isolated nucleic acid) in the host to produce the desired sequence.

In addition to the above, the nucleic acid molecule of the present invention can also be introduced into a chloroplast genome thereby enabling chloroplast expression.

A technique for introducing exogenous nucleic acid sequences to the genome of the chloroplasts is known. This technique involves the following procedures. First, plant cells are chemically treated so as to reduce the number of chloroplasts per cell to about one. Then, the exogenous nucleic acid is introduced via particle bombardment into the cells with the aim of introducing at least one exogenous nucleic acid molecule into the chloroplasts. The exogenous nucleic acid is selected such that it gets integrated into the chloroplast's genome via homologous recombination which is readily effected by enzymes inherent to the chloroplast. To this end, the exogenous nucleic acid includes, in addition to a gene of interest, at least one nucleic acid stretch which is derived from the chloroplast's genome. In addition, the exogenous nucleic acid includes a selectable marker, which serves by sequential selection procedures to ascertain that all or substantially all of the copies of the chloroplast genomes following such selection will include the exogenous nucleic acid. Further details relating to this technique are found in U.S. Pat. Nos. 4,945,050; and 5,693,507 which are incorporated herein by reference.

Regardless of the method of transformation, propagation or regeneration, the present invention also contemplates a transgenic plant exogenously expressing the polynucleotide/nucleic acid agent of the invention.

According to a specific embodiment, the transgenic plant exogenously expresses a polynucleotide having a nucleic acid sequence at least, 80%, 85%, 90%, 95% or even 100% identical to SEQ ID NOs: 1-56, 11874, 174-201, 80-125, 220-235, 162-168, 256-259, 262-2086, 2087-3910 11616, 11615, 11910-11939, 11956-11958, 11875-11904 or 3911 (Tables 1, 3, 5 and 7), wherein the nucleic acid sequence is capable of regulating abiotic stress tolerance of the plant.

According to further embodiments, the exogenous polynucleotide encodes a precursor of the nucleic acid sequence.

According to yet further embodiments, the stem-loop precursor is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or even 100% identical to SEQ ID NOs: 1-56, 11874, 174-201, 80-125, 220-235, 162-168, 256-259, 262-2086, 2087-3910 11616, 11615, 11910-11939, 11956-11958, 11875-11904 or 3911 (Tables 1, 3, 5 and 7). More specifically the exogenous polynucleotide is selected from the group consisting of SEQ ID NO: 1-56, 11874, 174-201, 80-125, 220-235, 162-168, 256-259, 262-2086, 2087-3910 11616, 11615, 11910-11939, 11956-11958, 11875-11904 or 3911 (precursor and mature sequences of unregulated Tables 1, 3, 5 and 7).

Alternatively, there is provided a transgenic plant exogenously expressing a polynucleotide which downregulates an activity or expression of a gene encoding a miRNA molecule having a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 57-79, 202-219, 126-161, 236-255, 169-173, 260-261, 3953-5114, 5117-6277, 11905-11909, 11940-11955, 11959-11961 or 6278 (downregulated Tables 2, 4, 6, 8) or homologs thereof which are at least at least 80%, 85%, 90% or 95% identical to SEQ ID NOs: 57-79, 202-219, 126-161, 236-255, 169-173, 260-261, 3953-5114, 5117-6277, 11905-11909, 11940-11955, 11959-11961 or 6278 (downregulated Tables 2, 4, 6 and 8).

More specifically, the transgenic plant expresses the nucleic acid agent of Tables 14 or 16. Alternatively, the transgenic plant expresses the nucleic acid agent of Tables 15 or 17.

It will be appreciated that the present teachings also relate to nucleic acid constructs and transgenic plants expressing same which comprise a nucleic acid sequence at least 80%, 85%, 90%, 95% or even 100% identical to SEQ ID NOs: 57-79, 202-219, 126-161, 236-255, 169-173, 260-261, 3953-5114, 5117-6277, 11905-11909, 11940-11955, 11959-11961 or 6278 (Tables 2, 4, 6 and 8), wherein the nucleic acid sequence is capable of regulating abiotic stress tolerance of the plant.

Alternatively or additionally there is provided a transgenic plant exogenously expressing a polynucleotide encoding a polypeptide having an amino acid sequence at least 80%, 85%, 90%, 95% or even 100% homologous to SEQ ID NOs: 9591-10364 (polypeptides of Table 10), wherein the polypeptide is capable of regulating abiotic stress tolerance of the plant.

Alternatively or additionally there is provided a transgenic plant exogenously expressing a polynucleotide encoding a polypeptide having an amino acid sequence at least 80%, 85%, 90%, 95% or even 100% homologous to SEQ ID NOs: 6315-8129 (polypeptides of Table 9), wherein the polypeptide is capable of regulating abiotic stress tolerance of the plant.

Alternatively or additionally there is provided a transgenic plant exogenously expressing a polynucleotide which downregulates an activity or expression of a polypeptide having an amino acid sequence at least 80%, 85%, 90%, 95% or even 100% homologous to SEQ ID NOs: 6315-10963 (targets of Tables 9 and 10), wherein the polypeptide is capable of regulating abiotic stress tolerance of the plant.

Also contemplated are hybrids of the above described transgenic plants. A “hybrid plant” refers to a plant or a part thereof resulting from a cross between two parent plants, wherein one parent is a genetically engineered plant of the invention (transgenic plant expressing an exogenous miRNA sequence or a precursor thereof). Such a cross can occur naturally by, for example, sexual reproduction, or artificially by, for example, in vitro nuclear fusion. Methods of plant breeding are well-known and within the level of one of ordinary skill in the art of plant biology.

Since abiotic stress tolerance, nitrogen use efficiency as well as yield, vigor or biomass of the plant can involve multiple genes acting additively or in synergy (see, for example, in Quesda et al., Plant Physiol. 130:951-063, 2002), the invention also envisages expressing a plurality of exogenous polynucleotides in a single host plant to thereby achieve superior effect on the efficiency of nitrogen use, yield, vigor and biomass of the plant.

Expressing a plurality of exogenous polynucleotides in a single host plant can be effected by co-introducing multiple nucleic acid constructs, each including a different exogenous polynucleotide, into a single plant cell. The transformed cell can then be regenerated into a mature plant using the methods described hereinabove. Alternatively, expressing a plurality of exogenous polynucleotides in a single host plant can be effected by co-introducing into a single plant-cell a single nucleic-acid construct including a plurality of different exogenous polynucleotides. Such a construct can be designed with a single promoter sequence which can transcribe a polycistronic messenger RNA including all the different exogenous polynucleotide sequences. Alternatively, the construct can include several promoter sequences each linked to a different exogenous polynucleotide sequence.

The plant cell transformed with the construct including a plurality of different exogenous polynucleotides can be regenerated into a mature plant, using the methods described hereinabove.

Alternatively, expressing a plurality of exogenous polynucleotides can be effected by introducing different nucleic acid constructs, including different exogenous polynucleotides, into a plurality of plants. The regenerated transformed plants can then be cross-bred and resultant progeny selected for superior yield or tolerance traits as described above, using conventional plant breeding techniques.

Expression of the miRNAs of the present invention or precursors thereof can be qualified using methods which are well known in the art such as those involving gene amplification e.g., PCR or RT-PCR or Northern blot or in-situ hybridization.

According to some embodiments of the invention, the plant expressing the exogenous polynucleotide(s) is grown under stress (nitrogen or abiotic) or normal conditions (e.g., biotic conditions and/or conditions with sufficient water, nutrients such as nitrogen and fertilizer). Such conditions, which depend on the plant being grown, are known to those skilled in the art of agriculture, and are further, described above.

According to some embodiments of the invention, the method further comprises growing the plant expressing the exogenous polynucleotide(s) under abiotic stress or nitrogen limiting conditions. Non-limiting examples of abiotic stress conditions include, water deprivation, drought, excess of water (e.g., flood, waterlogging), freezing, low temperature, high temperature, strong winds, heavy metal toxicity, anaerobiosis, nutrient deficiency, nutrient excess, salinity, atmospheric pollution, intense light, insufficient light, or UV irradiation, etiolation and atmospheric pollution.

Thus, the invention encompasses plants exogenously expressing the polynucleotide(s), the nucleic acid constructs of the invention.

Methods of determining the level in the plant of the RNA transcribed from the exogenous polynucleotide are well known in the art and include, for example, Northern blot analysis, reverse transcription polymerase chain reaction (RT-PCR) analysis (including quantitative, semi-quantitative or real-time RT-PCR) and RNA-m situ hybridization.

The sequence information and annotations uncovered by the present teachings can be harnessed in favor of classical breeding. Thus, sub-sequence data of those polynucleotides described above, can be used as markers for marker assisted selection (MAS), in which a marker is used for indirect selection of a genetic determinant or determinants of a trait of interest (e.g., tolerance to abiotic stress). Nucleic acid data of the present teachings (DNA or RNA sequence) may contain or be linked to polymorphic sites or genetic markers on the genome such as restriction fragment length polymorphism (RFLP), microsatellites and single nucleotide polymorphism (SNP), DNA fingerprinting (DFP), amplified fragment length polymorphism (AFLP), expression level polymorphism, and any other polymorphism at the DNA or RNA sequence.

Examples of marker assisted selections include, but are not limited to, selection for a morphological trait (e.g., a gene that affects form, coloration, male sterility or resistance such as the presence or absence of awn, leaf sheath coloration, height, grain color, aroma of rice); selection for a biochemical trait (e.g., a gene that encodes a protein that can be extracted and observed; for example, isozymes and storage proteins); selection for a biological trait (e.g., pathogen races or insect biotypes based on host pathogen or host parasite interaction can be used as a marker since the genetic constitution of an organism can affect its susceptibility to pathogens or parasites).

The polynucleotides described hereinabove can be used in a wide range of economical plants, in a safe and cost effective manner.

Plant lines exogenously expressing the polynucleotide of the invention can be screened to identify those that show the greatest increase of the desired plant trait.

Thus, according to an additional embodiment of the present invention, there is provided a method of evaluating a trait of a plant, the method comprising: (a) expressing in a plant or a portion thereof the nucleic acid construct; and (b) evaluating a trait of a plant as compared to a wild type plant of the same type; thereby evaluating the trait of the plant.

Thus, the effect of the transgene (the exogenous polynucleotide) on different plant characteristics may be determined any method known to one of ordinary skill in the art.

Thus, for example, tolerance to limiting nitrogen conditions may be compared in transformed plants {i.e., expressing the transgene) compared to non-transformed (wild type) plants exposed to the same stress conditions (other stress conditions are contemplated as well, e.g. water deprivation, salt stress e.g. salinity, suboptimal temperatureosmotic stress, and the like), using the following assays.

Methods of qualifying plants as being tolerant or having improved tolerance to abiotic stress or limiting nitrogen levels are well known in the art and are further described hereinbelow.

Fertilizer use efficiency—To analyze whether the transgenic plants are more responsive to fertilizers, plants are grown in agar plates or pots with a limited amount of fertilizer, as described, for example, in Yanagisawa et al (Proc Natl Acad Sci USA. 2004; 101:7833-8). The plants are analyzed for their overall size, time to flowering, yield, protein content of shoot and/or grain. The parameters checked are the overall size of the mature plant, its wet and dry weight, the weight of the seeds yielded, the average seed size and the number of seeds produced per plant. Other parameters that may be tested are: the chlorophyll content of leaves (as nitrogen plant status and the degree of leaf verdure is highly correlated), amino acid and the total protein content of the seeds or other plant parts such as leaves or shoots, oil content, etc. Similarly, instead of providing nitrogen at limiting amounts, phosphate or potassium can be added at increasing concentrations. Again, the same parameters measured are the same as listed above. In this way, nitrogen use efficiency (NUE), phosphate use efficiency (PUE) and potassium use efficiency (KUE) are assessed, checking the ability of the transgenic plants to thrive under nutrient restraining conditions.

Nitrogen use efficiency—To analyze whether the transgenic plants (e.g., Arabidopsis plants) are more responsive to nitrogen, plant are grown in 0.75-3 millimolar (mM, nitrogen deficient conditions) or 10, 6-9 mM (optimal nitrogen concentration). Plants are allowed to grow for additional 25 days or until seed production. The plants are then analyzed for their overall size, time to flowering, yield, protein content of shoot and/or grain/seed production. The parameters checked can be the overall size of the plant, wet and dry weight, the weight of the seeds yielded, the average seed size and the number of seeds produced per plant. Other parameters that may be tested are: the chlorophyll content of leaves (as nitrogen plant status and the degree of leaf greenness is highly correlated), amino acid and the total protein content of the seeds or other plant parts such as leaves or shoots and oil content. Transformed plants not exhibiting substantial physiological and/or morphological effects, or exhibiting higher measured parameters levels than wild-type plants, are identified as nitrogen use efficient plants.

Nitrogen Use efficiency assay using plantlets—The assay is done according to Yanagisawa-S. et al. with minor modifications (“Metabolic engineering with Dofl transcription factor in plants: Improved nitrogen assimilation and growth under low-nitrogen conditions” Proc. Natl. Acad. Sci. USA 101, 7833-7838). Briefly, transgenic plants which are grown for 7-10 days in 0.5×MS [Murashige-Skoog] supplemented with a selection agent are transferred to two nitrogen-limiting conditions: MS media in which the combined nitrogen concentration (NH₄NO₃and KNO₃) was 0.75 mM (nitrogen deficient conditions) or 6-15 mM (optimal nitrogen concentration). Plants are allowed to grow for additional 30-40 days and then photographed, individually removed from the Agar (the shoot without the roots) and immediately weighed (fresh weight) for later statistical analysis. Constructs for which only T1 seeds are available are sown on selective media and at least 20 seedlings (each one representing an independent transformation event) are carefully transferred to the nitrogen-limiting media. For constructs for which T2 seeds are available, different transformation events are analyzed. Usually, 20 randomly selected plants from each event are transferred to the nitrogen-limiting media allowed to grow for 3-4 additional weeks and individually weighed at the end of that period. Transgenic plants are compared to control plants grown in parallel under the same conditions. Mock-transgenic plants expressing the uidA reporter gene (GUS) under the same promoter or transgenic plants carrying the same promoter but lacking a reporter gene are used as control.

Nitrogen determination—The procedure for N (nitrogen) concentration determination in the structural parts of the plants involves the potassium persulfate digestion method to convert organic N to NO₃⁻(Purcell and King 1996 Argon. J. 88:111-113, the modified Cd⁻ mediated reduction of NO₃⁻to NO₂⁻(Vodovotz 1996 Biotechniques 20:390-394) and the measurement of nitrite by the Griess assay (Vodovotz 1996, supra). The absorbance values are measured at 550 nm against a standard curve of NaNO₂. The procedure is described in details in Samonte et al. 2006 Agron. J. 98:168-176.

Tolerance to abiotic stress (e.g. tolerance to drought or salinity) can be evaluated by determining the differences in physiological and/or physical condition, including but not limited to, vigor, growth, size, or root length, or specifically, leaf color or leaf area size of the transgenic plant compared to a non-modified plant of the same species grown under the same conditions. Other techniques for evaluating tolerance to abiotic stress include, but are not limited to, measuring chlorophyll fluorescence, photosynthetic rates and gas exchange rates. Further assays for evaluating tolerance to abiotic stress are provided hereinbelow and in the Examples section which follows.

Drought tolerance assay—Soil-based drought screens are performed with plants overexpressing the polynucleotides detailed above. Seeds from control Arabidopsis plants, or other transgenic plants overexpressing nucleic acid of the invention are germinated and transferred to pots. Drought stress is obtained after irrigation is ceased. Transgenic and control plants are compared to each other when the majority of the control plants develop severe wilting. Plants are re-watered after obtaining a significant fraction of the control plants displaying a severe wilting. Plants are ranked comparing to controls for each of two criteria: tolerance to the drought conditions and recovery (survival) following re-watering.

Salinity tolerance assay—Transgenic plants with tolerance to high salt concentrations are expected to exhibit better germination, seedling vigor or growth in high salt. Salt stress can be effected in many ways such as, for example, by irrigating the plants with a hyperosmotic solution, by cultivating the plants hydroponically in a hyperosmotic growth solution (e.g., Hoagland solution with added salt), or by culturing the plants in a hyperosmotic growth medium [e.g., 50 Murashige-Skoog medium (MS medium) with added salt]. Since different plants vary considerably in their tolerance to salinity, the salt concentration in the irrigation water, growth solution, or growth medium can be adjusted according to the specific characteristics of the specific plant cultivar or variety, so as to inflict a mild or moderate effect on the physiology and/or morphology of the plants (for guidelines as to appropriate concentration see, Bernstein and Kafkafi, Root Growth Under Salinity Stress In: Plant Roots, The Hidden Half 3rd ed. Waisel Y, Eshel A and Kafkafi U. (editors) Marcel Dekker Inc., New York, 2002, and reference therein).

For example, a salinity tolerance test can be performed by irrigating plants at different developmental stages with increasing concentrations of sodium chloride (for example 50 mM, 150 mM, 300 mM NaCl) applied from the bottom and from above to ensure even dispersal of salt. Following exposure to the stress condition the plants are frequently monitored until substantial physiological and/or morphological effects appear in wild type plants. Thus, the external phenotypic appearance, degree of chlorosis and overall success to reach maturity and yield progeny are compared between control and transgenic plants. Quantitative parameters of tolerance measured include, but are not limited to, the average wet and dry weight, growth rate, leaf size, leaf coverage (overall leaf area), the weight of the seeds yielded, the average seed size and the number of seeds produced per plant. Transformed plants not exhibiting substantial physiological and/or morphological effects, or exhibiting higher biomass than wild-type plants, are identified as abiotic stress tolerant plants.

Osmotic tolerance test—Osmotic stress assays (including sodium chloride and PEG assays) are conducted to determine if an osmotic stress phenotype was sodium chloride-specific or if it was a general osmotic stress related phenotype. Plants which are tolerant to osmotic stress may have more tolerance to drought and/or freezing. For salt and osmotic stress experiments, the medium is supplemented for example with 50 mM, 100 mM, 200 mM NaCl or 15%, 20% or 25% PEG.

Cold stress tolerance—One way to analyze cold stress is as follows. Mature (25 day old) plants are transferred to 4° C. chambers for 1 or 2 weeks, with constitutive light. Later on plants are moved back to greenhouse. Two weeks later damages from chilling period, resulting in growth retardation and other phenotypes, are compared between control and transgenic plants, by measuring plant weight (wet and dry), and by comparing growth rates measured as time to flowering, plant size, yield, and the like.

Heat stress tolerance—One way to measure heat stress tolerance is by exposing the plants to temperatures above 34° C. for a certain period. Plant tolerance is examined after transferring the plants back to 22° C. for recovery and evaluation after 5 days relative to internal controls (non-transgenic plants) or plants not exposed to neither cold or heat stress.

The biomass, vigor and yield of the plant can also be evaluated using any method known to one of ordinary skill in the art. Thus, for example, plant vigor can be calculated by the increase in growth parameters such as leaf area, fiber length, rosette diameter, plant fresh weight, oil content, seed yield and the like per time.

As mentioned, the increase of plant yield can be determined by various parameters. For example, increased yield of rice may be manifested by an increase in one or more of the following: number of plants per growing area, number of panicles per plant, number of spikelets per panicle, number of flowers per panicle, increase in the seed filling rate, increase in thousand kernel weight (1000-weight), increase oil content per seed, increase starch content per seed, among others. An increase in yield may also result in modified architecture, or may occur because of modified architecture. Similarly, increased yield of soybean may be manifested by an increase in one or more of the following: number of plants per growing area, number of pods per plant, number of seeds per pod, increase in the seed filling rate, increase in thousand seed weight (1000-weight), reduce pod shattering, increase oil content per seed, increase protein content per seed, among others. An increase in yield may also result in modified architecture, or may occur because of modified architecture.

Thus, the present invention is of high agricultural value for increasing tolerance of plants to nitrogen deficiency or abiotic stress as well as promoting the yield, biomass and vigor of commercially desired crops.

According to another embodiment of the present invention, there is provided a food or feed comprising the plants or a portion thereof of the present invention.

In a further aspect the invention, the transgenic plants of the present invention or parts thereof are comprised in a food or feed product (e.g., dry, liquid, paste). A food or feed product is any ingestible preparation containing the transgenic plants, or parts thereof, of the present invention, or preparations made from these plants. Thus, the plants or preparations are suitable for human (or animal) consumption, i.e. the transgenic plants or parts thereof are more readily digested. Feed products of the present invention further include an oil or a beverage adapted for animal consumption.

It will be appreciated that the transgenic plants, or parts thereof, of the present invention may be used directly as feed products or alternatively may be incorporated or mixed with feed products for consumption. Furthermore, the food or feed products may be processed or used as is. Exemplary feed products comprising the transgenic plants, or parts thereof, include, but are not limited to, grains, cereals, such as oats, e.g. black oats, barley, wheat, rye, sorghum, corn, vegetables, leguminous plants, especially soybeans, root vegetables and cabbage, or green forage, such as grass or hay.

As used herein the term “about” refers to ±10%.

The terms “comprises”, “comprising”, “includes”, “including”, “having” and their conjugates mean “including but not limited to”.

The term “consisting of” means “including and limited to”.

The term “consisting essentially of” means that the composition, method or structure may include additional ingredients, steps and/or parts, but only if the additional ingredients, steps and/or parts do not materially alter the basic and novel characteristics of the claimed composition, method or structure.

As used herein, the singular form “a”, “an” and “the” include plural references unless the context clearly dictates otherwise. For example, the term “a compound” or “at least one compound” may include a plurality of compounds, including mixtures thereof.

Throughout this application, various embodiments of this invention may be presented in a range format. It should be understood that the description in range format is merely for convenience and brevity and should not be construed as an inflexible limitation on the scope of the invention. Accordingly, the description of a range should be considered to have specifically disclosed all the possible subranges as well as individual numerical values within that range. For example, description of a range such as from 1 to 6 should be considered to have specifically disclosed subranges such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6 etc., as well as individual numbers within that range, for example, 1, 2, 3, 4, 5, and 6. This applies regardless of the breadth of the range.

Whenever a numerical range is indicated herein, it is meant to include any cited numeral (fractional or integral) within the indicated range. The phrases “ranging/ranges between” a first indicate number and a second indicate number and “ranging/ranges from” a first indicate number “to” a second indicate number are used herein interchangeably and are meant to include the first and second indicated numbers and all the fractional and integral numerals therebetween.

As used herein the term “method” refers to manners, means, techniques and procedures for accomplishing a given task including, but not limited to, those manners, means, techniques and procedures either known to, or readily developed from known manners, means, techniques and procedures by practitioners of the chemical, pharmacological, biological, biochemical and medical arts.

It is appreciated that certain features of the invention, which are, for clarity, described in the context of separate embodiments, may also be provided in combination in a single embodiment. Conversely, various features of the invention, which are, for brevity, described in the context of a single embodiment, may also be provided separately or in any suitable subcombination or as suitable in any other described embodiment of the invention. Certain features described in the context of various embodiments are not to be considered essential features of those embodiments, unless the embodiment is inoperative without those elements.

Various embodiments and aspects of the present invention as delineated hereinabove and as claimed in the claims section below find experimental support in the following examples.

EXAMPLES

Reference is now made to the following examples, which together with the above descriptions illustrate some embodiments of the invention in a non limiting fashion.

Generally, the nomenclature used herein and the laboratory procedures utilized in the present invention include molecular, biochemical, microbiological and recombinant DNA techniques. Such techniques are thoroughly explained in the literature. See, for example, “Molecular Cloning: A laboratory Manual” Sambrook et al., (1989); “Current Protocols in Molecular Biology” Volumes I-III Ausubel, R. M., ed. (1994); Ausubel et al., “Current Protocols in Molecular Biology”, John Wiley and Sons, Baltimore, Md. (1989); Perbal, “A Practical Guide to Molecular Cloning”, John Wiley & Sons, New York (1988); Watson et al., “Recombinant DNA”, Scientific American Books, New York; Birren et al. (eds) “Genome Analysis: A Laboratory Manual Series”, Vols. 1-4, Cold Spring Harbor Laboratory Press, New York (1998); methodologies as set forth in U.S. Pat. Nos. 4,666,828; 4,683,202; 4,801,531; 5,192,659 and 5,272,057; “Cell Biology: A Laboratory Handbook”, Volumes I-III Cellis, J. E., ed. (1994); “Current Protocols in Immunology” Volumes I-III Coligan J. E., ed. (1994); Stites et al. (eds), “Basic and Clinical Immunology” (8th Edition), Appleton & Lange, Norwalk, Conn. (1994); Mishell and Shiigi (eds), “Selected Methods in Cellular Immunology”, W. H. Freeman and Co., New York (1980); available immunoassays are extensively described in the patent and scientific literature, see, for example, U.S. Pat. Nos. 3,791,932; 3,839,153; 3,850,752; 3,850,578; 3,853,987; 3,867,517; 3,879,262; 3,901,654; 3,935,074; 3,984,533; 3,996,345; 4,034,074; 4,098,876; 4,879,219; 5,011,771 and 5,281,521; “Oligonucleotide Synthesis” Gait, M. J., ed. (1984); “Nucleic Acid Hybridization” Hames, B. D., and Higgins S. J., eds. (1985); “Transcription and Translation” Hames, B. D., and Higgins S. J., Eds. (1984); “Animal Cell Culture” Freshney, R. I., ed. (1986); “Immobilized Cells and Enzymes” IRL Press, (1986); “A Practical Guide to Molecular Cloning” Perbal, B., (1984) and “Methods in Enzymology” Vol. 1-317, Academic Press; “PCR Protocols: A Guide To Methods And Applications”, Academic Press, San Diego, Calif. (1990); Marshak et al., “Strategies for Protein Purification and Characterization—A Laboratory Course Manual” CSHL Press (1996); all of which are incorporated by reference as if fully set forth herein. Other general references are provided throughout this document. The procedures therein are believed to be well known in the art and are provided for the convenience of the reader. All the information contained therein is incorporated herein by reference.

Example 1
Differential Expression of miRNAs in Soybean Plant Under Abiotic Stress Versus Optimal Conditions

Plant Material

Glycine max seeds (soy) were obtained from Taam-Teva shop (Israel). Plants were grown at 28° C. under a 16 hours light:8 hours dark regime.

Drought Induction

Plants were grown under standard conditions as described above until seedlings were four weeks old. Next, plants were divided into two groups: control plants were irrigated with tap water twice a week and drought-treated plants received no irrigation. The experiment continued for five days, after which plants were harvested for RNA extraction.

Salt Induction

For salinity induction, irrigation with regular water was substituted by irrigation with 300 mM NaCl solution in the stress group, for an overall of 3 irrigations for a ten-day period.

Heat Shock Induction

For induction of heat shock, the stress group plants were exposed to a high temperature (37° C.) for one hour.

Total RNA Extraction

Total RNA of leaf samples from eight biological repeats were extracted using the mirVana™ kit (Ambion, Austin, Tex.) by pooling tissues from 2-4 plants to one biological repeat. RNA analysis was performed on plant tissue samples from both experimental and control groups.

Microarray Design

Custom microarrays were manufactured by Agilent Technologies by in situ synthesis. A microarray based on Sanger version 16 was designed and consisted of a total 4602 non-redundant DNA 45-nucleotide long probes for all known plant small RNAs, with 1875 sequences (40.7%) from miRBase (http://wwwDOTmirbaseDOTorg/indexDOTshtml) and 2678 sequences (58DOT2%) from PMRD (http://bioinformaticsDOTcauDOTeduDOTcn/PMRD/), with each probe being printed in triplicate. Control and spike probes account for the remaining sequences on the microarray.

Results

Wild type soybean plants were allowed to grow at standard, optimal conditions or stress conditions for a period of time as specified above, at the end of which they were evaluated for stress tolerance. Three to four plants from each group were grouped as a biological repeat. Four to eight biological repeats were obtained for each group, and RNA was extracted from leaf tissue. The expression level of the soybean small RNAs was analyzed by high throughput microarray to identify small RNAs that were differentially expressed between the experimental groups.

Tables 1-6 below present sequences that were found to be differentially expressed in soybean grown under drought conditions (lasting five days), high salt conditions (lasting ten days) or heat shock conditions (lasting one hour), compared to optimal growth conditions. To clarify, the sequence of an up-regulated miRNA is induced under stress conditions and the sequence of a down-regulated miRNA is repressed under stress conditions.

TABLE 1

Differentially Expressed (Up-regulated) Small RNAs in Soybean Plants

Growing under Drought (5 days) versus Optimal Conditions.

Mature Sequence
Stem Loop

Fold-

miR Name
(SEQ ID NO)
Sequence (SEQ ID NO)
p-value
Change

ahy-miR3514-5p
AGGATTCTGTAT
ACAATAGAAGGATTCTGTATTAAC
5.90E−06
1.97 (+)

TAACGGTGGA (1)
GGTGGACATGATTTATCTCGTTTTT

AAAGATATCTTTGCATTTCATATG

AGATTTAAAGTTTTTATTGGTAAT

ATAAATCTCACATGAAATTTAAAT

TTATATTTTAAAGTTAAGATAAAG

TCATGTCACCGTTAATACAGAATC

CTTCAATTATATTTAGTCAGGGG

(174)

aly-miR831-5p
AGAAGAGGTAC
AAGTGCTACAAGAATGTATAGTCT
1.10E−05
1.88 (+)

AAGGAGATGAG
TAGAGTCTCAAGAAGAGGTACAA

A (2)
GGAGATGAGAAGTGAATCACTGA

AACAAGTGGTTCTGGTTTGTGGAT

CAGTATGGTTTACCCAAAACACGT

GTTTGGTGCTTCACTTCTAAACTCC

TCGTACTCTTCTTGGGATTCTATGA

CTTACACTTGTTGATTT (175)

aqc-miR159
TTTGGACTGAA
GGAGTCTTTCCAGCCCAAAACAGC
3.00E−03
1.51 (+)

GGGAGCTCTA (3)
TTCTTGATCTTCTTGAAAACTTCTG

TTTGGACTGAAGGGAGCTCTAA

(176)

ath-miR157a
TTGACAGAAGA
GTGTTGACAGAAGATAGAGAGCA
1.00E−03
1.70 (+)

TAGAGAGCAC (4)
CAGATGATGAGATACAATTCGGAG

CATGTTCTTTGCATCTTACTCCTTT

GTGCTCTCTAGCCTTCTGTCATCAC

C (177)

ath-miR159b
TTTGGATTGAAG
GGAAGAGCTCCTTGAAGTTCAATG
6.80E−03
1.63 (+)

GGAGCTCTT (5)
GAGGGTTTAGCAGGGTGAAGTAA

AGCTGCTAAGCTATGGATCCCATA

AGCCTTATCAAATTCAATATAATT

GATGATAAGGTTTTTTTTATGGAT

GCCATATCTCAGGAGCTTTCACTT

ACCCCTTTAATGGCTTCACTCTTCT

TTGGATTGAAGGGAGCTCTTCATC

TCTC (178)

ath-miR159c
TTTGGATTGAAG
GTGTAACAGAAGGAGCTCCCTTCC
1.20E−03
1.65 (+)

GGAGCTCCT (6)
TCCAAAACGAAGAGGACAAGATT

TGAGGAACTAAAATGCAGAATCTA

AGAGTTCATGTCTTCCTCATAGAG

AGTGCGCGGTGTTAAAAGCTTGAA

GAAAGCACACTTTAAGGGGATTGC

ACGACCTCTTAGATTCTCCCTCTTT

CTCTACATATCATTCTCTTCTCTTC

GTTTGGATTGAAGGGAGCTCCTTT

TCTTCTTC (179)

ath-miRf10068-
CACCGGTGGAG
GGACTTCTCATCTTCTTTCTTAGCC
2.00E−08
2.09 (+)

akr
GAGTGAGAG (7)
GCCGGTGCTCCAGCTCCACCACCG

TGTCCTCCAACATTACCGTGGCTT

CCAGTTCCACCGGTGGAGGAGTGA

GAGTGGGAAGTTT (11875)

ath-miRf10240-
ATCGAAGGAGA
GATTTCTCGTCCTCCGGCAATCCTT
1.90E−05
1.90 (+)

akr
TGGAGGACG (8)
CGAACTCATCTTCATCCCAGTAAT

CGAAGGAGATGGAGGACGAAGGC

TTC (11876)

ath-miRf10451-
AAGAAGGAGGA
CTCTAGATCTCAACAGGTTTCCTC
2.30E−07
2.19 (+)

akr
ACAACCTGTTG
CTCCTTCTTTCTATTTAGCTACTTG

(9)
GTTTCAATTGTTTCAAGCCTAGGT

AAGCATATGTAAAAAAGAGACAA

TTGAAACCAAGTAACTAAATAGAA

AGAAGGAGGAACAACCTGTTGAG

ATCTAGAG (11877)

ath-miRf10687-
TTAGCTGAAGA
TTTGTTTGTTTAGCTGAAGAAGCA
3.70E−06
1.84 (+)

akr
AGCAGAGGAG (10)
GAGGAGTCGGCATTGGGGCACAG

TCACTCATCGATGCTGCAATGGGT

AAGTCCTCTGCATACTTTTGCTGA

GATAGGAATAGA (11878)

ath-miRf10701-
TGCAGTTCCTGG
GGTGCCGCTGCAGTTCCTGGAGGT
1.30E−08
2.49 (+)

akr
AGGTGGAGGA (11)
GGAGGAGGTGGTGGTGGGGCCAC

TGCAGCTCTTGGAGGTGGAGGCGG

TGGAGGTGGAGCCGCTATAGTTGT

TGGAAGTGGAGGAGGTGGCGGTG

GTGGT (11879)

ath-miRf10751-
CTTGTGGAGAG
TTGTAATTTCTTGTGGAGAGGAAG
7.80E−08
2.10 (+)

akr
GAAGCAAGA (12)
CAAGAGGATGTGCTTGGTTGTGGA

AATATAGGGCCCTTAAAATATATT

CATCGTATTCACTCACATAACAAA

AATTCCACAAGTAAGCACATCATC

TTGCTTCCTCCACAAGAAATTACA

A (11880)

ath-miRf10924-
TGAGGCGTATC
TGAGGCGTATCAGGAGGTAGTGTT
5.70E−04
1.73 (+)

akr
AGGAGGTAGT (13)
CTTGGTGGGACAATTTGTGTTGTA

TGTTTCA (11881)

ath-miRf11021-
GAGGTTTGCGA
GATGTTGGAGGTTTGCGATGAGAA
1.80E−04
1.60 (+)

akr
TGAGAAAGAG (14)
AGAGATTGGCCGGAAGAATTATCA

GCCATCAACATCGAGATTGTGAGA

TAATCGGAAGACCTGTAATTGTGA

AGGTAACTCTTTCTCATCTGCAAA

TCTCAACTGTC (11882)

ath-miRf11037-
TCATCGGAGAA
TTGTCTCTGTTCATCGGAGAAACA
1.30E−07
2.78 (+)

akr
ACAGAGGAGC (15)
GAGGAGCAAGACGTTTCAAACGG

TTCTTGGCTCATAATTTGCTTCTCT

GTTACCTTGGATGACAAGAAAGAC

AA (11883)

ath-miRf11042-
GGAAGAGGCAG
AGGGAGCCAGGGAAGAGGCAGTG
2.20E−03
1.56 (+)

akr
TGCATGGGTA (16)
CATGGGTAGAGACAAAACAGAGT

CGTTTAATGTTTTAGTAAACTCAA

TCCATGCTCTGCTTGTTCCCTGTCT

CTCT (11884)

ath-miRf11045-
TTTCTTGTGGAG
TTGTAATTTCTTGTGGAGGAAGCA
8.60E−11
3.85 (+)

akr
GAAGCAAGAT (17)
AGATGATGTGCTTACTTGTGGAAT

TTTTGTTATGTGAGTGAATACGAT

GAATATATTTTAAGGGCCCTATAT

TTCCACAACCAAGCACATCCTCTT

GCTTCCTCTCCACAAGAAATTACA

A (11885)

csi-miR3946
TTGTAGAGAAA
ATAAAGATGATGATGACAATGAAT
1.80E−04
1.68 (+)

GAGAAGAGAGC
TTTGTAGAGAAAGAGAAGAGAGC

AC (18)
ACAAACTTTTTGCTGAAAGTAGCT

TTGATTCGATGTGTATCGGTTCAT

AGATAATGAGTTTTCAAGTCTATT

TTAATAGAATACTAAAAGTTAGCT

CTAAAAATC (180)

gma-miR156g
ACAGAAGATAG
TGAACAATATCTTGAACAGTTTGT
2.00E−04
1.76 (+)

AGAGCACAG (19)
TGACAGAAGATAGAGAGCACAGG

TGATCATACCCAAAAAAGCTTTTG

TGTGTGAGCAGTTTTGTGCTCTCTA

TCTTCTGTCAATGTACTTCTCA (181)

gma-miR157c
TGACAGAAGAC
TGACAGAAGACTAGAGAGCACAA
3.90E−04
1.58 (+)

TAGAGAGCAC (20)
AGGAGTGAGATGCCATTCCCTTTC

ATGCATTTCATCATCAGTGCTCTCT

ATCTTCTGTCAA (182)

gma-miR159a-3p
TTTGGATTGAAG
AATTAAAGGGGATTATGAAGTGG
8.40E−03
1.59 (+)

GGAGCTCTA (21)
AGCTCCTTGAAGTCCAATTGAGGA

TCTTACTGGGTGAATTGAGCTGCT

TAGCTATGGATCCCACAGTTCTAC

CCATCAATAAGTGCTTTTGTGGTA

GTCTTGTGGCTTCCATATTTGGGG

AGCTTCATTTGCCTTTATAGTATTA

ACCTTCTTTGGATTGAAGGGAGCT

CTACACCCTTCTCTTCTTTTCT (183)

iba-miR157
TTGACAGAAGA
TTGACAGAAGATAGAGAGCATGCT
6.40E−04
1.60 (+)

TAGAGAGCAT (22)
AGAAATTACATTGATAAGCTATGT

GGTTCAGAGACCAATCTTCTTATG

AGTTCCAATAAGGAGTTGGTTTGT

CCCCCCACTGGTATTATGTCTTCA

GGTTGACCCTTCACCATGAGAATC

ATATGTAATTCTCCGGCGGCGCTC

ATTGTGACCTGCCAATCGCCTCCG

GCAACTCCTCTTAGCTTCATCAAA

CTGGGCTAATTCATGAAACCTGCT

GCATTGCTGACAGAAGCGCTGTTG

AACTCCATTTATAAGTACT (184)

mdm-miR482a-5p
GGAATGGGCTG
GAGAAGAGGGAAAGGGAGATTGG
1.50E−04
1.62 (+)

TTTGGGAACA (23)
AGCTGCTGGAAGTTTTAGGAATGG

GCTGTTTGGGAACAAGGAAATTAC

CACAATAATTGTCTTGTGGGGTTT

CTTCCCAAGCCCGCCCATTCCTAT

GATTTCCAGCTGTTCCTCCCTTTCC

CTTGTCTC (185)

mtr-miR2119
TCAAAGGGAGG
TTTATTTTTTTTACACTAAGATACT
2.20E−07
2.64 (+)

TGTGGAGTAG (24)
CCCTACTTTCCTTTGATTGGAAATA

AAGAGAGACAAAAAGGTAAATTT

AATTTCTCTTCTTATGTCAATCAAA

GGGAGGTGTGGAGTAGGGTGTAA

AAAGTAAA (186)

osa-miR159e
ATTGGATTGAA
GATGAAGAAGAAGAGCTCCCTTTC
1.40E−03
1.67 (+)

GGGAGCTCCT (25)
GATCCAATTCAGGAGAGGAAGTG

GTAGGATGCAGCTGCCGGTTCATG

GATACCTCTGGAGTGCAGGGCAAA

TAGTCCTACCCTTTCATGGGTTTGC

ATGACTCGGGAGATGAACCCGCCA

TTGTCTTCCTCTATTGATTGGATTG

AAGGGAGCTCCTCTAGCTACAT

(187)

osa-miR159f
CTTGGATTGAA
GAAGAAGAAGACGAGCTCCCTTC
5.10E−03
1.75 (+)

GGGAGCTCTA (26)
GATCCAATCCAGGAGAGGAAGTG

GTAGGATGCAGCTGCCGGTTCATG

GATACCTCTGCAGTGCATGTCGTA

GGCTTGCACTTGCATGGGTTTGCA

TGACCCGGGAGATGAACCCACCAT

TGTCTTCCTCTTATGCTTGGATTGA

AGGGAGCTCTACACCTCTCTC (188)

osa-miR1858a
GAGAGGAGGAC
TCCCGTCATCGCTGCCGGCAAAGG
3.90E−04
1.63 (+)

GGAGTGGGGC (27)
GAGGGGGGGTGCCGCAACAAGGA

GAGGAGGACGGAGTGGGGCGAGT

GGAGCGTCAAAGGGGATGTCATC

GCCGCCGAATCTGCTCGTGGGACA

TCCCCTTCGATGCTCCACTCGCCCC

AATCCGTCCTCCTCTCCTTGTTGCG

GCACCCCCCTTCGCTGGCAGCGAC

GACGGCCTC (189)

osa-miR1874-3p
TATGGATGGAG
CCATAATCATCTATTAGTACAGTG
7.80E−10
4.36 (+)

GTGTAACCCGA
GTGAAGACATAGGGCTACTACACC

TG (28)
ATCCATAAGGGTTCGAATCTTCGA

TGTGCCTAGATAGGGTACAGTTGG

ATCCCATATGGATGGAGGTGTAAC

CCGATGCCTTTTACAAATAGATGG

TTATTTT (190)

osa-miR1879
GTGTTTGGTTTA
TCCAACCCATCCCACCTCGTCCCC
5.50E−06
1.91 (+)

GGGATGAGGTG
AAACCAAACACATGCACGCAAAT

G (29)
GGCTTGTTGAGGAATAAACATCTT

GCTCCCTTGCATTCTAAACTATGA

TATTCTTCAAGCATATGTGTTTGGT

TTAGGGATGAGGTGGGATGGGTTA

GGTCCA (191 (ATCCAACCCATCCCA

CCTCGTCCCCAAACCAAACACATG

CACGCAAATGGCTTGTTGAGGAAT

AAACATCTTGCTCCCTTGCATTCTA

AACTATGATATTCTTCAAGCATAT

GTGTTTGGTTTAGGGATGAGGTGG

GATGGGTTAGGTCCA (201)

osa-miRf10839-
CCTGTGACGTTG
CTGCGAGCCTCCAGCAGCGGCACA
1.90E−11
7.36 (+)

akr
GTGAAGGTG (30)
GGAGGAGGCCATTGCAGCTGTCAA

GGACGTTGAGAAACTCGCACTTGG

GCAGGAAGGGGAGCGAGGGGTCA

ACAAACGGGCGGCCTTCCCCTGTG

ACGTTGGTGAAGGTGTCGGAG

(11886)

osa-miRf11013-
GGTTTGCCGGA
CCAGCCATCCCTCTAGAGCCGGCG
7.70E−07
2.11 (+)

akr
GTTGGAGGAGA
AACTCCTCCCCTCCCCCCTCCCCCT

(31)
TCCACTCCCACCCCACCCCACCCC

GGGACCCTAACCCGTAGGGTCCTC

GCCGGCGCCAGAGAAGAAGAGGT

TTGCCGGAGTTGGAGGAGATGACA

TGG (11887)

osa-miRf11352-
AGGGATTTTGG
TATAATATAAGGGATTTTGGAAGG
1.20E−06
1.64 (+)

akr
AAGGAGGTGAC
AGGTGACATATTCTAGGACTATGT

A (32)
ATCTGGATCCAGAGATACTAGGAT

GTGTTACCTCCCTCTAAAATCCCTT

ATATTATG (11888)

osa-miRf11355-
GGTGGAGGTGG
GGTGGAGGTGGAGGTGGAGCTGT
7.60E−09
2.57 (+)

akr
AGCTGTGCCAA
GCCAAATAGGCCCTGAGTTGTATG

A (33)
CACCACCAGTTCAACCCAATAGCT

TAAGGGTCTGCTTGGCACAGCTCC

AGCTCCACGCAGCC (11889)

osa-miRf11595-
CATCGGTGTTGG
CATCGGTGTTGGAGGTGGCGGGGA
2.70E−06
2.10 (+)

akr
AGGTGGC (34)
CGAGGTGCTTCTCTAGAGCGGTGC

CACTACTGCCACCACCGTGGAATT

GACGAGGCACAATGCCCACCTCAC

CCTCCGCTGCCACTCTGCTGCCAC

CGATG (11890)

osa-miRf11649-
AAACCGTGCAA
TTACTTAAACCGTGCAAAGGAGGT
1.50E−09
4.07 (+)

akr
AGGAGGTCCC (35)
CCCATGGCAGTATTTGCACCCGTT

TTTACTAACGTGGCATCCTGTTGT

ACGGTTTTTTTTGACGCAAATACT

GCCATGGGACCTCTTTTGCATGGT

TTGAGTAA (11891)

osa-miRf11829-
ACGCGGAGGAG
GCACGCGGAGGAGGTGGTGTTCTC
2.80E−04
1.51 (+)

akr
GTGGTGTTCT (36)
GCCGGAGTACGAGGAGTTCGCCGT

CAGGAACGCCGCCCTCTGCGTCC

(11892)

ppt-miR895
GTAGCTTAGCG
GATTCATGTAATTATTGTTAACCTC
6.90E−13
12.97 (+)

AGGTGTTGGTA
TTTGTGTTCCGAGCTTTTATGATTG

(37)
GTAGCTTAGCGAGGTGTTGGTATG

ATACCAATCCCTGGTTTGCTTGTTC

CTAATTGAGTTATGCTTGCACTCA

AATCTAGGGGAGCGGTATTTTGGC

TCACTCGCAATGCTTTCATGTACC

CTTCCCGCATTATGAGTGCCACTT

GGCTCCATGGTGTGATATATAAGT

TTC (192)

psi-miR159
CTTGGATTGAA
TGGAGCTCCCTTCAGTCCAACCAA
9.90E−04
1.60 (+)

GGGAGCTCCA (38)
AGCTTGTGCGGCGGTGGTTCAGCT

GCTGATTCATGCATTCGACTGCCC

TGTCCGTGACTTTCCAGCAGCCTG

AATCAATCAATCTATCTCCATGAC

AGGATAGTGGTGTGCATGACGCAG

GAGATGTATTGTCACTGGACACGC

ATTCCTTGGATTGAAGGGAGCTCC

A (193)

pta-miR156a
CAGAAGATAGA
GATGACAGAAGATAGAGAGCACA
3.50E−04
1.58 (+)

GAGCACATC (39)
TCCGCTCACATGCCGGGACTCTGC

GTTTGAGGTGTATGTGGTCTCCAT

GATTCTGTCATC (194)

pta-miR156b
CAGAAGATAGA
GATGACAGAAGATAGAGAGCACA
1.80E−04
1.65 (+)

GAGCACAAC (40)
ACCGCTCAGATGCCGGCACTCTGC

GTTTGAGGTGTATGTGCTCTCGTT

GATTTTGTCATC (195)

ptc-miRf10148-
TGGTGCACCTG
CTAGTTCCGGAGCCCGGTGAACTT
7.40E−06
2.02 (+)

akr
GTGGTGGAG (41)
TATCACCACTTCCTGCTCCTCTTGG

CAAGCTTCCAGGTGGAGGAGGTG

GACGAGGTGGTCCACCAGGTGGA

GGAGGTGGTGGTGGTGGTGCACCT

GGTGGTGGAGGTGG (11893)

ptc-miRf10226-
TCCTTTGGGGAG
ATGGTTGGAGAAGCTTCCGATCTC
1.70E−06
1.88 (+)

akr
ATGGAGAGCTT
CCTCAAAGGCTTCCTCTATAATTG

(42)
CCTTACATGATGGCATTAGTGGAC

TCCTTTGGGGAGATGGAGAGCTTA

CTCCCCAT (11894)

ptc-miRf10271-
TTGGATTGAAG
GGGAGTGGAGCTCCTTGAAGTCCA
6.60E−03
1.65 (+)

akr
GGAGCTCTAA (43)
ATAGAGGTTCTTGCTGGGTAGATT

AAGCTGCTAAGCTATGGATCCACA

GTCCTATCTATCAACCGAAGGATA

GGTTTGCGGCTTGCATATCTCAGG

AGCTTTATTGCCTAATGTTAGATC

CCTTTTTGGATTGAAGGGAGCTCT

AAACCC (11895)

ptc-miRf10300-
TTTGGAAAGCA
TATACATATATCTCACTTGCTTTCT
2.70E−06
2.38 (+)

akr
AGTGAGGTG (44)
CAACTATCTCACTTTTCTTTTCAGA

TTTCAAAAAAACGACATCATGAGA

CAGTTTGGAAAGCAAGTGAGGTGT

GTGTATA (11896)

ptc-miRf10522-
TTGGGGAGCTG
TGACGGATTCGGAGAACAGCTGTC
1.20E−04
3.58 (+)

akr
GACTCTGGA (45)
GGTGTTGATGGTGGCTGTGGGCAG

AGGACATTTCAGAATTTGGGGAGC

TGGACTCTGGAGCAGTGG (11897)

ptc-miRf10619-
GTTGGGCTTGCT
TCTGCTTCGGGTGGCAGGTCTGGC
8.80E−07
2.47 (+)

akr
GCTGGAGGA (46)
GGTTGTAGAGGGGGCAGCGACGTT

GATGATCTTCGCTCCTGTTGGTTGC

CGTGGCGGTTGGGCTTGCTGCTGG

AGGAAGA (11898)

ptc-miRf10985-
CAGAAGATAGA
GTTGACAGAAGATAGAGAGCACT
3.20E−04
1.70 (+)

akr
GAGCACTGA (47)
GACGATGAAATGCATGGAGCTTAA

TTGCATCTCACTCCTTTGTGCTCTC

TAGTCTTCTGTCATC (11899)

ptc-miRf11757-
CTTGGTGAATG
TGGGACAGCTTGGTGAATGGTTGG
3.70E−07
1.84 (+)

akr
GTTGGGAGGAA
GAGGAATGTCTTTAATGTGGTTAT

T (48)
GCATCAGTGAAACTCTAGTAAGAT

TCTCTGTCCACTCCTCTGCATCCGG

CACTTCTCTTAACCGTGCACCTGCT

TTAACCA (11900)

ptc-miRf11844-
CCCAACTTGGA
GCGTCCAGACCCAACTTGGAGGTG
8.30E−04
1.73 (+)

akr
GGTGGGTGTGG
GGTGTGGACGCGTCCAACCCCAAG

(49)
TTGGGCGTGGATGCGTCCAGGCCT

AATTTCGAGTTGGGCGTAGACGC

(11901)

ptc-miRf11847-
GAAAGTGTGGA
ACTTTTCTACGAAAGTGTGGAGAA
4.30E−07
2.28 (+)

akr
GAAGGTTGCC (50)
GGTTGCCCCTAAAAAATCTTTTAT

GGCGACTTTCTCGACATTTTGGTA

GAAAAGT (11902)

ptc-miRf11855-
GGCAGAGCATG
CGGGGAACAGGCAGAGCATGGAT
5.90E−05
3.96 (+)

akr
GATGGAGCTA (51)
GGAGCTACTAACAGAAGTACTTGT

TTTGGCTCTACCCATGCACTGCCTC

TTCCCTG (11903)

sbi-miR159a
TTTGGATTGAAG
AGCGAAGCTCCTATCATTCCAATG
1.20E−02
1.59 (+)

GGAGCTCTG (52)
AAGGGCCCTTTTCATGGGTGGTTC

CGCTGCTCGTTCATGGTTCCCACT

ATCCTATCTCATCATGTATGTGTGT

ATGTACTCTAGAGGGCCCGAGAAG

AGATTCATGTGGTCGTCAGTCTTT

GAGATAGGCTTGTGGTTTGCATGA

CCGAGGAGCTGCACCGTCCCCTTG

CTGGCCGCTCTTTGGATTGAAGGG

AGCTCTGCA (196)

smo-miR1103-3p
TGGAAAAAGGA
GCCCATGAACAAGAGTGCACCCCC
7.50E−10
3.98 (+)

GGTGCATTCTTG
TTTCCAATCGGTTAAAGGTCTTAG

T (53)
GATAGTTGGAGTTTAAGCGTCCTT

GGGTTTGAATAGTACTGGGCTGGG

TGACCTCCCGGGAAGTCCAAATTC

AGGAGCTTACATTAACCCCAAGTA

TTCCAAAACGCTTAATCGATTGGA

AAAAGGAGGTGCATTCTTGTTCAT

AGGCCC (197)

smo-miR156b
CTGACAGAAGA
TGGACTGCTGCTGACAGAAGATAG
2.50E−04
1.62 (+)

TAGAGAGCAC (54)
AGAGCACAGACGTTTGGCTGCAAG

AGCGGAATCCATATCCAGCAGCTC

TGCGTTCGTGCTCTCTATTCTTCTG

TCATCAATCTTTCGA (198)

tae-miR2003
CGGTTGGGCTGT
CATTCGGATTCGCCATCATACGTC
7.90E−05
2.99 (+)

ATGATGGCGA (55)
CAACCGTGCATTTGATATGCATAT

ATATGCATCACGAGCAACGGTTGG

GCTGTATGATGGCGATACCGATTG

(199)

AGTGGGAGATGAAGGAGCCTTGC
7.80E−06
2.19 (+)

zma-miR482-5p
TGGGAGATGAA
ATCGATGTCACCGCCGGAGGAGCG

GGAGCCTT
CTCGCCTTCTTCGCGCACCGCCGC

(11874)
AATAGCCGCCCTCGGACCCCTCGC

CTCGCTCTTCCTTGTTCCTCCCATT

TT (11904)

* NA = not available

TABLE 2

Differentially Expressed (Down-regulated) Small RNAs in Soybean Plants

Growing under Drought (5 days) versus Optimal Conditions.

Mature

Sequence

Fold-

miR Name
(SEQ ID NO:)
Stem Loop Sequence (SEQ ID NO:)
p-Value
Change

aly-miR396a-
GTTCAATAAAGCT
TCTACGTGACCCTCTCTGTATTCTTCCACA
7.20E−08
2.93 (−)

3p
GTGGGAAG (57)
GCTTTCTTGAACTGCAAAACTTCTTCAGAT

TTGTTTTTTTTATATATATGTCTTACGCAT

AAAATAGTGTTTTTGTTCACATCTCTGCTC

GATTGATTTGCGGTTCAATAAAGCTGTGG

GAAGATACGGACAGAGTCAAAGA (202)

aly-miR396b-
GCTCAAGAAAGCT
GAAGAAGAAGAAGAAGATCCTGGTCATA
2.10E−08
4.25 (−)

3p
GTGGGAAA (58)
TTTTTCCACAGCTTTCTTGAACTTTCTTTTT

CATTTCCATTGTTTTTTTTTTTCTAAACCAA

AAAAAAAGATCTCTAAAATTTAGCATTTT

GGAAACAAAGAAGAAGCTCAAGAAAGCT

GTGGGAAAACATGACAATTCAGGGTTTTA

CTCCATTGATTC (203)

bdi-miR2508
ATTGAGTGCAGCG
GCAAAGGCATCATTGAGTGCAGCGTTGAT
1.60E−02
1.73 (−)

TTGATGAAC (59)
GAACAGGGGCCAGGCGACCGGCGGCCGG

TCCGGTTCGGTTCACCGGCGCTGCACACA

GTGACGCCCTTGC (204)

ctr-miR171
TTGAGCCGCGTCA
ATCGACGGTTGAAGGGGAGAGTTGTAAAA
7.00E−04
1.90 (−)

ATATCTCC (60)
TGAAATCATCAAGGTATTGGCGCGCCTCA

ATTTAAAGACGTGGTTAAATGGGCATGAT

TAGCCATGTATTTTCATTGAGCCGCGTCAA

TATCTCCTTAATTATTTTGTAACTCTCTCCT

CTATATCCTCGCCTTCGGTATGCAGCTGCT

CCTCGATACATATGAGGATTCAGAAACAG

ACAAAGGCGGTAGAAGTAATCTTCATCAA

TATTATTGAAGCAGGAAACATAACGGCAA

GTTTTAAGACCCGTTTGGGGCATGTGGGG

TCTCATTTTGATGTTAATGAAGTGAAAACT

TGTATTTTCCCTCAAACATTCACTCACTCC

AGGCCGGCAGGAACAAC (205)

gma-
TCTCATTCCATACA
CAGTGTTTGGCAGAGGTGTATGGAGTGAG
3.20E−03
1.61 (−)

miR1507a
TCGTCTGA (61)
AGAAGGGAAAGGGTATTTTCCGATTCTGT

CGTTACTCTCTTCCCTCTCTCATTCCATAC

ATCGTCTGACGAACGTATC (206)

gma-miR159d
AGCTGCTTAGCTAT
GGGTGAATTGAGCTGCTTAGCTATGGATC
7.30E−07
2.63 (−)

GGATCCC (62)
CCACAGTTCTACCCATCAATAAGTGCTTTT

GTGGTAGTCTTGTGGCTTCCATATCTGGGG

AGCTTCATTTGCCT (207)

gma-miR396d
AAGAAAGCTGTGG
GGTCATGCTTTTCCACAGCTTTCTTGAACT
8.90E−06
2.12 (−)

GAGAATATGGC
TCTTATGCATCTTATATCTCTCCACTTCCA

(63)
GCATTTTAAGCCCTAGAAGCTCAAGAAAG

CTGTGGGAGAATATGGCAA (208)

gma-
AAGTGATGACGTG
TTGTTAGTTGCCTTTCGTTAAGTGATGACG
2.10E−03
1.86 (−)

miR4371b
GTAGACGGAGT
TGGTAGACGGAGTGCCGTGTCATTATGCC

(64)
TTGTCACAGACACCTCATTGCTACATCATC

ACCGCTAATGATATGGCACTGACGTGTTA

GTGATTGGTGTGATGACGTGACACTCTAT

CTGCCACATCATCACCTAACGGAAGACAA

CTAACGA (209)

gma-
TACGCAGGAGAGA
AAGGTTTGCTACGCAGGAGAGATGACGCT
6.10E−05
2.57 (−)

miR4376-5p
TGACGCTGT (65)
GTCCCTTGCACCCATCCTAGCTTCCCTTGA

GTAGGTAAGAGCAAGGCCAGCCAGCATC

ATATCTCCTGCATAGTAAACCTT (210)

gma-
ACGGGTCGCTCTCA
CTTTGATCTGGGTGAGAGAAACGCGTATC
1.40E−03
3.07 (−)

miR4416a
CCTAGG (66)
GATGGATTGGGTTCAGTTCTGGTCTCACA

CGGTTTGTTCTAACAATTTGTACTGACTGT

GTTTTGATCGATACGGGTCGCTCTCACCTA

GGCCAGAGTTGC (211

gma-
TCTTCCCAATTCCG
TCAGAATTTGTGGGAATGGGCTGATTGGG
1.30E−05
2.05 (−)

miR482a-3p
CCCATTCCTA (67
AAGCAATGTGTGCTGGTGCAATGCATTTA

ATTTCTTCCCAATTCCGCCCATTCCTATGA

TTTCTGA (212)

gma-
TATGGGGGGATTG
GGTATGGGGGGATTGGGAAGGAATATCCA
3.20E−08
1.71 (−)

miR482b-5p
GGAAGGAAT (68)
TAAGCAAAATATGCTATTTCTTCCCTACAC

CTCCCATACC (213)

gso-miR169g*
TCGGCAAGTTGGC
AGCCAAGGAUGACUUGCCGGCAUUAGCC
1.20E−05
2.46 (−)

CTTGGCT (69)
AAGUGAAUGAGCAUCAUAUAUAUAUAUA

UAUAUAUAUAUGACUCAUGUUCUUGUCG

GCAAGUUGGCCUUGGCU (11905)

gso-miR482a
TCTTCCCTACACCT
GGGGAAGGCATGGGTATGGGGGGATTGG
1.60E−02
1.74 (−)

CCCATAC (70
GAAGGAATATCCATAAGCAAAATATGCTA

TTTCTTCCCTACACCTCCCATACCACTGTT

TTTCCT (214)

osa-
GTCTTATAACCTGA
TACTACCTCCATTTCAGGTTATAAGACTTT
2.10E−02
1.84 (−)

miRf11996-
AACGGGGG (71)
CTAGTGTTGCTCACATTCATATATATGTTA

akr

ATAAATTCATTAACATATAGAAAGTCTTA

TAACCTGAAACGGGGGAAGTA (11906)

ppt-miR166m
TCGGACCAGGCAT
GCCGAGAACAGAGATTGTGTAGCTCAGCT
2.20E−05
1.77 (−)

CATTCCTT (72)
GTAAGGAATGTGGCATGGCTCGATGCTGT

TTGAGCATGTCAAGTTCAGCCTCGGACCA

GGCATCATTCCTTTCATCTCAGTTACACAT

TTGACATCCAGGA (215)

pta-miR166c
CCGGACCAGGCTT
ACCAATCGAATCCGGACCAGGCTTCATCC
8.30E−04
1.59 (−)

CATCCCAG (73)
CAGGCATCTGGACCCAATCGACAGCAGCT

CCTTTAGCCTTTGAAAGGAACTCTGTCAA

GGTCTCCTCTGCTATAGACAGGAGTCCAG

CGGGGCTAGCATCTCTTGGGGGATGCTGA

GGTGTTGGATTATGTTGGT (216)

ptc-miR166p
TCGGACCAGGCTC
TAAGGTTGAGAGGAACGCTGTCTGGGTCG
4.70E−05
1.77 (−)

ptc-
CATTCCTT (74)
AGGTCATGGAGGCCATGATTATACATAAA

TGGCATTATCTGATGACAGCCCAGATAAT

CGATGCACCTGTCTTGAACCTAAATGATT

CTCGGACCAGGCTCCATTCCTTCCAACCAT

(217)

AAGATGGAGAAGC
GTGTGTGAGCAAGATGGAGAAGCAGGGC
6.10E−04
1.56 (−)

miRf11079-
AGGGCACGTGC
ACGTGCACTACTAACTCATGCACACAGAG

akr
(75)
AGGGAGACGCATTTCTTGCTGGAGTTACG

AGTTACGACTCTTACCTACTATTGATTTTG

TTAGCTCCAGTGAGTTAGTTATTCATGTGC

CTGTCTTCCTCATCATGATCACTAC

(11907)

ptc-
CAAGGCTCTGATA
CTTGGTCATCAAGGCTCTGATACCATGTC
1.70E−05
1.81 (−)

miRf11396-
CCATGTCAA (76)
AAAGAATCATATTTTGAGACCTTATCTAA

akr

CAGCTTAAGCTATTGGGTTGAGATGGTTC

CTTGACATGATATCAGAGCCTTGATGACG

AAG (11908)

ptc-
CAAGGCTCTGATA
GCTTGGTCATCAAGGCTCTGATACCATGTT
2.30E−04
1.63 (−)

miRf11669-
CCATGTT (77)
AAAGAACCATCTCAACCTAATACCATGTT

akr

AGAGAATAATATAAATCATATCTAGAGAC

TTTACCTAACAGCTTAAGCTATTGGCCTAT

TGGATTAGTATGGTTCTTTGACATGGTATC

AGAGCCTTGATAACCAAGT (11909)

vvi-miR394b
TTGGCATTCTGTCC
ACAGAGTTTATTGGCATTCTGTCCACCTCC
6.70E−03
1.55 (−)

ACCTCC (78)
CATCTCTTGAAAATCTCTCTTTTCTCTCTG

TGGAGGTGGGCATACTGCCAACCAAGCTC

TGTT (218)

zma-
GTTCAATAAAGCT
AGATGGCCTTCTTTGTGATCTTCCACAGCT
2.10E−08
4.76 (−)

miR396b-3p
GTGGGAAA (79)
TTCTTGAACTGCATCTCTCAGAGGAGCGG

CAGCTTCAACTCCTCCACCCGCATCAGCA

GGTGCATGCAGTTCAATAAAGCTGTGGGA

AACTGCAGAGAGAGGCCAG (219)

TABLE 3

Differentially Expressed (Up-regulated) Small RNAs in Soybean Plants

Growing under High Salt (10 days) versus Optimal Conditions.

Mature

Sequence

Fold-

miR Name
(SEQ ID NO:)
Stem Loop Sequence (SEQ ID NO:)
p-Value
Change

ppt-miR895
GTAGCTTAGC
GATTCATGTAATTATTGTTAACCTCTTTGTG
7.20E−10
9.36 (+)

GAGGTGTTGG
TTCCGAGCTTTTATGATTGGTAGCTTAGCGA

TA (80)
GGTGTTGGTATGATACCAATCCCTGGTTTGC

TTGTTCCTAATTGAGTTATGCTTGCACTCAA

ATCTAGGGGAGCGGTATTTTGGCTCACTCG

CAATGCTTTCATGTACCCTTCCCGCATTATG

AGTGCCACTTGGCTCCATGGTGTGATATAT

AAGTTTC (220)

ptc-
TTTGGAAAGC
TATACATATATCTCACTTGCTTTCTCAACTA
1.40E−07
6.02 (+)

miRf10300-
AAGTGAGGTG
TCTCACTTTTCTTTTCAGATTTCAAAAAAAC

akr
(81)
GACATCATGAGACAGTTTGGAAAGCAAGTG

AGGTGTGTGTATA (11910)

osa-
CCTGTGACGTT
CTGCGAGCCTCCAGCAGCGGCACAGGAGGA
6.20E−09
5.21 (+)

miRf10839-
GGTGAAGGTG
GGCCATTGCAGCTGTCAAGGACGTTGAGAA

akr
(82)
ACTCGCACTTGGGCAGGAAGGGGAGCGAG

GGGTCAACAAACGGGCGGCCTTCCCCTGTG

ACGTTGGTGAAGGTGTCGGAG (11911)

smo-
TGGAAAAAGG
GCCCATGAACAAGAGTGCACCCCCTTTCCA
6.50E−08
5.20 (+)

miR1103-3p
AGGTGCATTCT
ATCGGTTAAAGGTCTTAGGATAGTTGGAGT

TGT (83)
TTAAGCGTCCTTGGGTTTGAATAGTACTGG

GCTGGGTGACCTCCCGGGAAGTCCAAATTC

AGGAGCTTACATTAACCCCAAGTATTCCAA

AACGCTTAATCGATTGGAAAAAGGAGGTGC

ATTCTTGTTCATAGGCCC (221)

osa-
AAACCGTGCA
TTACTTAAACCGTGCAAAGGAGGTCCCATG
9.70E−08
5.10 (+)

miRf11649-
AAGGAGGTCC
GCAGTATTTGCACCCGTTTTTACTAACGTGG

akr
C (84)
CATCCTGTTGTACGGTTTTTTTTGACGCAAA

TACTGCCATGGGACCTCTTTTGCATGGTTTG

AGTAA (11912)

osa-miR1874-
TATGGATGGA
CCATAATCATCTATTAGTACAGTGGTGAAG
6.50E−09
4.75 (+)

3p
GGTGTAACCC
ACATAGGGCTACTACACCATCCATAAGGGT

GATG (85)
TCGAATCTTCGATGTGCCTAGATAGGGTAC

AGTTGGATCCCATATGGATGGAGGTGTAAC

CCGATGCCTTTTACAAATAGATGGTTATTTT

(222)

ptc-
GTTGGGCTTGC
TCTGCTTCGGGTGGCAGGTCTGGCGGTTGT
2.00E−08
3.70 (+)

miRf10619-
TGCTGGAGGA
AGAGGGGGCAGCGACGTTGATGATCTTCGC

akr
(86)
TCCTGTTGGTTGCCGTGGCGGTTGGGCTTGC

TGCTGGAGGAAGA (11913)

osa-
GCTGGAGGAT
GCCGGCTAGTACAATCGAATCCACTAGCAC
7.50E−08
3.48 (+)

miRf10362-
GCGACGGTGC
CGAGGCTTGGGTCACTAGATCCCGTGGCCC

akr
T (87)
TAGCCTAATTGCTGGAGGATGCGACGGTGC

TTGTGAGC (11914)

ahy-miR3514-
AGGATTCTGT
ACAATAGAAGGATTCTGTATTAACGGTGGA
2.40E−08
3.43 (+)

5p
ATTAACGGTG
CATGATTTATCTCGTTTTTAAAGATATCTTT

GA (88)
GCATTTCATATGAGATTTAAAGTTTTTATTG

GTAATATAAATCTCACATGAAATTTAAATTT

ATATTTTAAAGTTAAGATAAAGTCATGTCA

CCGTTAATACAGAATCCTTCAATTATATTTA

GTCAGGGG (223)

mtr-miR2119
TCAAAGGGAG
TTTATTTTTTTTACACTAAGATACTCCCTAC
2.00E−07
2.89 (+)

GTGTGGAGTA
TTTCCTTTGATTGGAAATAAAGAGAGACAA

G (89)
AAAGGTAAATTTAATTTCTCTTCTTATGTCA

ATCAAAGGGAGGTGTGGAGTAGGGTGTAA

AAAGTAAA (224)

osa-
GGTGGAGGTG
GGTGGAGGTGGAGGTGGAGCTGTGCCAAAT
2.40E−07
2.85 (+)

miRf11355-
GAGCTGTGCC
AGGCCCTGAGTTGTATGCACCACCAGTTCA

akr
AAA (90)
ACCCAATAGCTTAAGGGTCTGCTTGGCACA

GCTCCAGCTCCACGCAGCC (11915)

osa-
CATCGGTGTTG
CATCGGTGTTGGAGGTGGCGGGGACGAGGT
8.90E−06
2.77 (+)

miRf11595-
GAGGTGGC
GCTTCTCTAGAGCGGTGCCACTACTGCCAC

akr
(91)
CACCGTGGAATTGACGAGGCACAATGCCCA

CCTCACCCTCCGCTGCCACTCTGCTGCCACC

GATG (11916)

ptc-
CCCAACTTGG
GCGTCCAGACCCAACTTGGAGGTGGGTGTG
1.20E−05
2.75 (+)

miRf11844-
AGGTGGGTGT
GACGCGTCCAACCCCAAGTTGGGCGTGGAT

akr
GG (92)
GCGTCCAGGCCTAATTTCGAGTTGGGCGTA

GACGC (11916)

ptc-
GAAAGTGTGG
ACTTTTCTACGAAAGTGTGGAGAAGGTTGC
2.50E−06
2.70 (+)

miRf11847-
AGAAGGTTGC
CCCTAAAAAATCTTTTATGGCGACTTTCTCG

akr
C (93)
ACATTTTGGTAGAAAAGT (11917)

ath-
TTTCTTGTGGA
TTGTAATTTCTTGTGGAGGAAGCAAGATGA
4.40E−07
2.59 (+)

miRf11045-
GGAAGCAAGA
TGTGCTTACTTGTGGAATTTTTGTTATGTGA

akr
T (94)
GTGAATACGATGAATATATTTTAAGGGCCC

TATATTTCCACAACCAAGCACATCCTCTTGC

TTCCTCTCCACAAGAAATTACAA (11918)

ath-
GTGGGAGGAC
AAACATGTGGGAGGACTCCAAGTGTGGTTA
8.90E−07
2.51 (+)

miRf10702-
TCCAAGTGTG
TATCCTCGGTATTATCTCGATGTGAACCACA

akr
(95)
CTTGGAGTCCTCCCACATGTTT (11919)

ath-
TGCAGTTCCTG
GGTGCCGCTGCAGTTCCTGGAGGTGGAGGA
1.40E−06
2.40 (+)

miRf10701-
GAGGTGGAGG
GGTGGTGGTGGGGCCACTGCAGCTCTTGGA

akr
A (96)
GGTGGAGGCGGTGGAGGTGGAGCCGCTATA

GTTGTTGGAAGTGGAGGAGGTGGCGGTGGT

GGT (11920)

osa-miR1869
TGAGAACAAT
AAGGAACACCTGAGAACAATAGGCATGGG
2.20E−05
2.27 (+)

AGGCATGGGA
AGGTATTGGGAAAACACAGGAACATATTGT

GGTA (97)
GACCCCTAATTTTAAAGGGAAATAATGGTT

GAGGCTTTCCTCCATGTTCCCATGCCTAATG

CTCTTAGGTGCTCTTTTT (225)

ptc-
TGGTGCACCT
CTAGTTCCGGAGCCCGGTGAACTTTATCAC
2.80E−05
2.22 (+)

miRf10148-
GGTGGTGGAG
CACTTCCTGCTCCTCTTGGCAAGCTTCCAGG

akr
(98)
TGGAGGAGGTGGACGAGGTGGTCCACCAG

GTGGAGGAGGTGGTGGTGGTGGTGCACCTG

GTGGTGGAGGTGG (11921)

osa-miR1879
GTGTTTGGTTT
TCCAACCCATCCCACCTCGTCCCCAAACCA
5.80E−05
2.21 (+)

AGGGATGAGG
AACACATGCACGCAAATGGCTTGTTGAGGA

TGG (99)
ATAAACATCTTGCTCCCTTGCATTCTAAACT

ATGATATTCTTCAAGCATATGTGTTTGGTTT

AGGGATGAGGTGGGATGGGTTAGGTCCA (226)

ATCCAACCCATCCCACCTCGTCCCCAAAC

CAAACACATGCACGCAAATGGCTTGTTGAG

GAATAAACATCTTGCTCCCTTGCATTCTAAA

CTATGATATTCTTCAAGCATATGTGTTTGGT

TTAGGGATGAGGTGGGATGGGTTAGGTCCA

(235)

ath-
GGTGGTGGAA
TAGGGAATATCTTGATCTTTCCACCATCTAC
4.90E−06
2.21 (+)

miRf10148-
AGATCAAGAT
AAAGAATAAAAAAAAAGCTTCCAATATTAC

akr
(100)
TAGGTATTTGGTGGTGGAAAGATCAAGATA

TTCCTTA (11922)

osa-
GGTTTGCCGG
CCAGCCATCCCTCTAGAGCCGGCGAACTCC
4.70E−06
2.21 (+)

miRf11013-
AGTTGGAGGA
TCCCCTCCCCCCTCCCCCTTCCACTCCCACC

akr
GA (101)
CCACCCCACCCCGGGACCCTAACCCGTAGG

GTCCTCGCCGGCGCCAGAGAAGAAGAGGTT

TGCCGGAGTTGGAGGAGATGACATGG (11923)

ath-
ATGGTGGTAC
TCCTCGACTTCCTGGTAGAGTGGTGTGATCG
8.60E−05
2.18 (+)

miRf10209-
TCGGCCAGGT
AGTGATGGTCAGGTGTGGAGGTGATGATAC

akr
GGT (102)
TCGACCAGGTGGTCAAGTGAAGTGATCAAG

TGACTCTCATGGTGGTACTCGGCCAGGTGG

TCGAGTGG (11924)

ath-
TGAGGCGTAT
TGAGGCGTATCAGGAGGTAGTGTTCTTGGT
1.50E−04
2.18 (+)

miRf10924-
CAGGAGGTAG
GGGACAATTTGTGTTGTATGTTTCA (11925)

akr
T (103)

1.90E−06
2.15 (+)

ppt-miR1220a
TTCCGGTGGTG
ACTTCTTGCACTCCTCTATCTCCCTCGGCAC

AGGAAGATAG
CTGCACAGTGATTTTCTCAATATCTTCACGT

(104)
TGGTGGCCACGTTCGAACATATCCCATGCG

GGCAACTCCGGCGTAGGTGTACACGGCCAG

CGTTGCTTACCATCTGGAGGATACCCTTGCT

CAAACCTACGACTCTGTTCCGGTGGTGAGG

AAGATAGAGGAGTTCAAGAAGT (227)

osa-
CGCGCCGACG
CGCCGTCTCCCTCGCCGTCGCCGGCGTCGCC
5.40E−06
2.12 (+)

miRf11341-
ATGACGGTGG
GGAGATGACGAGAAGACGTGCCCCGGCGC

akr
AGT (105)
GCCGACGATGACGGTGGAGTCGGCG (11926)

osa-
AGGGATTTTG
TATAATATAAGGGATTTTGGAAGGAGGTGA

miRf11352-
GAAGGAGGTG
CATATTCTAGGACTATGTATCTGGATCCAG
2.30E−06
2.11 (+)

akr
ACA (106)
AGATACTAGGATGTGTTACCTCCCTCTAAA

ATCCCTTATATTATG (11927)

osa-miR2055
TTTCCTTGGGA
AGAAGATGGAGGCACCAGCCCAAGGAAAC
7.20E−04
2.09 (+)

AGGTGGTTTC
ACAGACATTGACACGCAATTCAAGGAGAAG

(107)
ATTGCGTCCTACTTTTTCCTTGGGAAGGTGG

TTTCTCTTCT (228)

ath-
ATCGAAGGAG
GATTTCTCGTCCTCCGGCAATCCTTCGAACT
9.80E−07
2.03 (+)

miRf10240-
ATGGAGGACG
CATCTTCATCCCAGTAATCGAAGGAGATGG

akr
(108)
AGGACGAAGGCTTC (11928)

ath-
CACCGGTGGA
GGACTTCTCATCTTCTTTCTTAGCCGCCGGT
6.30E−07
1.99 (+)

miRf10068-
GGAGTGAGAG
GCTCCAGCTCCACCACCGTGTCCTCCAACAT

akr
(109)
TACCGTGGCTTCCAGTTCCACCGGTGGAGG

AGTGAGAGTGGGAAGTTT (11929)

ghr-miR2950
TGGTGTGCAG
CATGGGTTTATGTTATATTCCATCTCTTGCA
5.70E−06
1.98 (+)

GGGGTGGAAT
CACTGGACTAGCCAGCTTTTTGTTGGCTTCA

A (110)
GCTTCAGGTTGGTGTGCAGGGGGTGGAATA

CATCATTGATATCATG (229)

ath-
ACTTGGGTGG
TGGTAAGTGATAATCATTACCACCCAAGCT
7.90E−04
1.98 (+)

miRf10368-
TGCTGATTAT
AACATTCAAACCAAAAACCAGTTTAAGTTA

akr
(111)
ACTTGGGTGGTGCTGATTATCACTTGTCG

(11930)

ath-
GGTGGTGAAG
GGCGGTGGTGAAGAAGCATGGTTTGGAAAT
9.30E−05
1.93 (+)

miRf10763-
AAGCATGGTT
CTCACAGCCTGGATTAGAGCCATATGAAGG

akr
(112)
GCTCACATGGGAGATGACCAAGAAAAGAG

ACGACACTGAAGTCCACAAGTTAGAATTAT

ATAGTGAAGTTTCTACATTATTTTCTCACCA

CCGCT (11931)

ptc-
CATCTAGGTG
TCTTACCATTGGACCACCTACTAGATGATTA
4.30E−04
1.92 (+)

miRf10734-
GTGGTCCAGT
AAAACTACATCATCTAACCATCTAGGTGGT

akr
G (113)
GGTCCAGTGGTAAGA (11932)

osa-
ACGCGGAGGA
GCACGCGGAGGAGGTGGTGTTCTCGCCGGA
4.10E−05
1.90 (+)

miRf11829-
GGTGGTGTTCT
GTACGAGGAGTTCGCCGTCAGGAACGCCGC

akr
(114)
CCTCTGCGTCC (11933)

zma-miR482-
TGGGAGATGA
AGTGGGAGATGAAGGAGCCTTGCATCGATG
4.20E−05
1.88 (+)

5p
AGGAGCCTT
TCACCGCCGGAGGAGCGCTCGCCTTCTTCG

(115)
CGCACCGCCGCAATAGCCGCCCTCGGACCC

CTCGCCTCGCTCTTCCTTGTTCCTCCCATTTT

(230)

osa-
TGGAAAGTTG
ATGTGATGGAGATGCGATGGAAAGTTGGGA
1.30E−05
1.86 (+)

miR1850.1
GGAGATTGGG
GATTGGGGGAAGTTGTGTGTGAACTAAACG

G (116)
TGGATTGGGGCCCTGTTTAGTTCACATCAAT

CTTCCTCCAAATTCCCAACTTTTCATCACAT

CACAATCACAT (231)

osa-miR1881
AATGTTATTGT
AACCAAGTTAAAATGCTCATAGGTATGAAC
2.00E−03
1.76 (+)

AGCGTGGTGG
AAGCATCAATGTTATTGTAGCGTGGTGGTG

TGT (117)
TGACCTCTGTGCACGTAAGCTTGAGGCAGC

AAGTTCGACTCCTTCAAAAGGAAGATTTGT

ACCGCTGGGGAAGTGCCAGAAGAAAAGAA

CAAGGAAGACTTGCTAGCAGGACAAAAGG

ACGGTAAACTTGGAAAAAAAAAGGTCCAG

AAGAAAAGAACAAAGAAGAACTGCTTGAA

GGAGTCGAACTTGCTACCTCAAGCTTGCGT

GCACATAGGTCACACCACTACGCTACAATA

ACGTTGATGCTTGTTCAGACCTGTGAGCATT

TTAACTTGGTT (232)

ptc-
TCCTTTGGGGA
ATGGTTGGAGAAGCTTCCGATCTCCCTCAA
9.60E−05
1.74 (+)

miRf10226-
GATGGAGAGC
AGGCTTCCTCTATAATTGCCTTACATGATGG

akr
TT (118)
CATTAGTGGACTCCTTTGGGGAGATGGAGA

GCTTACTCCCCAT (11934)

ptc-
CTTGGTGAAT
TGGGACAGCTTGGTGAATGGTTGGGAGGAA
3.90E−05
1.73 (+)

miRf11757-
GGTTGGGAGG
TGTCTTTAATGTGGTTATGCATCAGTGAAAC

akr
AAT (119)
TCTAGTAAGATTCTCTGTCCACTCCTCTGCA

TCCGGCACTTCTCTTAACCGTGCACCTGCTT

TAACCA (11935)

ath-
GAGGTTTGCG
GATGTTGGAGGTTTGCGATGAGAAAGAGAT
1.50E−03
1.66 (+)

miRf11021-
ATGAGAAAGA
TGGCCGGAAGAATTATCAGCCATCAACATC

akr
G (120)
GAGATTGTGAGATAATCGGAAGACCTGTAA

TTGTGAAGGTAACTCTTTCTCATCTGCAAAT

CTCAACTGTC (11936)

ath-
TGGCGGTGGA
GTGGATACTGTTCTGGTGGAGGATACTTCA
6.50E−04
1.65 (+)

miRf10633-
TACTTCTTGAT
CCGGCGGATGAGGGTAAGTCTTGATCGGTG

akr
CGG (121)
GTGGATACTTCACCGGTGGATGCTCGTATG

GTGGCGGTGGATACTTCTTGATCGGTGGTG

GATAC (11937)

ptc-
TTGGCGGTGA
GAGAAACGCTCTAATTAATCATCGTTATGC
1.90E−04
1.63 (+)

miRf10132-
TTGAACGGAG
CACGTGTCTATTTACGGATAACGCAACGCT

akr
GGT (122)
ACTAAATCGCGAATTTTAGTTTGAGTGGAA

GATCTTGGCCGTTGGATTGGCGGTGATTGA

ACGGAGGGTTGATC (11938)

ptc-
CAACTTAGAG
GCCCTTAACCAACTTAGAGTTGGGGGTGGG
5.20E−03
1.61 (+)

miRf11315-
TTGGGGGTGG
CACGTCATGGGTCAACCTAGGGTTGGTCTC

akr
(123)
GGACTCGCCCTTGCTCAACTTAGAGTTGGG

TACGGGC (11939)

aly-miR831-
AGAAGAGGTA
AAGTGCTACAAGAATGTATAGTCTTAGAGT
1.60E−03
1.61 (+)

5p
CAAGGAGATG
CTCAAGAAGAGGTACAAGGAGATGAGAAG

AGA (124)
TGAATCACTGAAACAAGTGGTTCTGGTTTG

TGGATCAGTATGGTTTACCCAAAACACGTG

TTTGGTGCTTCACTTCTAAACTCCTCGTACT

CTTCTTGGGATTCTATGACTTACACTTGTTG

ATTT (233)

csi-miR3948
TGGAGTGGGA
AGGAGTGTGGAGTGGGAGTGGGAGTAGGG
7.10E−05
1.55 (+)

GTGGGAGTAG
TGTTTACTTAGACTAAATGAAAGTATGGAT

GGTG (125)
TATCAATCAGAATCCTAATTATTTGTTTACT

TTGTCTTGGATTGGGAGTAAATTATTTTAAA

TTATAATTTTATCCTTATGTACAAAATTATA

A (234)

TABLE 4

Differentially Expressed (Down-regulated) Small RNAs in Soybean Plants

Growing under High Salt (10 days) versus Optimal Conditions.

Mature Sequence

Fold-

miR Name
(SEQ ID NO:)
Stem Loop Sequence (SEQ ID NO:)
p-Value
Change

aly-miR160c-3p
GCGTACAAGGAG
CATATAATAGTTTGTCGTCGTTATGCCTGGCT
7.60E−04
1.59 (−)

CCAAGCATG (126)
CCCTGTATGCCACGAGTGGATACCGATTTTGT

TATAAAATCGGCTGCCGGTGGCGTACAAGGA

GCCAAGCATGACCATAAGCATATG (236)

aly-miR396a-3p
GTTCAATAAAGCT
TCTACGTGACCCTCTCTGTATTCTTCCACAGC
1.20E−09
5.50 (−)

GTGGGAAG (127)
TTTCTTGAACTGCAAAACTTCTTCAGATTTGT

TTTTTTTATATATATGTCTTACGCATAAAATA

GTGTTTTTGTTCACATCTCTGCTCGATTGATT

TGCGGTTCAATAAAGCTGTGGGAAGATACGG

ACAGAGTCAAAGA (237)

aly-miR396b-3p
GCTCAAGAAAGC
GAAGAAGAAGAAGAAGATCCTGGTCATATTT
9.00E−07
3.80 (−)

TGTGGGAAA (128)
TTCCACAGCTTTCTTGAACTTTCTTTTTCATTT

CCATTGTTTTTTTTTTTCTAAACCAAAAAAAA

AGATCTCTAAAATTTAGCATTTTGGAAACAA

AGAAGAAGCTCAAGAAAGCTGTGGGAAAAC

ATGACAATTCAGGGTTTTACTCCATTGATTC

(238)

ath-miRf10197-
CACTCGACCAAG
GGTGAAGACACTCGACCTCGTGGTCGAGTGA
2.90E−07
3.71 (−)

akr
GGGGTCGAGTGA
TGTGATCGAGTGGTGGTCAGAAGATGGAGAT

(129)
GAAGTCACTCGACCAAGGGGGTCGAGTGATG

TGATC (11940)

ath-miRf10239-
CGCCTTGCATCAA
TCGGGCTCGGATTCGCTTGGTGCAGGTCGGG
1.00E−03
2.17 (−)

akr
CTGAATC (130)
AACCAATTCGGCTGACACAGCCTCGTGACTT

TTAAACCTTTATTGGTTTGTGAGCAGGGATTG

GATCCCGCCTTGCATCAACTGAATCGGATCC

TCGA (11941)

ath-miRf10279-
ACTCAGCCTGGG
TGATGGTGATACTCGACATCCAGGTAGAGTG
7.80E−06
4.99 (−)

akr
GGTCGAGTGAT
ATGAGGTCGAGTAGAGGTCTGGCAATGGGAT

(131)
GAAGTCACTCAGCCTGGGGGTCGAGTGATGT

GATCG (11942)

bna-miR2111b-
TAATCTGCATCCT
GCACTTGATGAGGAACTGGTAATCTGCATCC
4.40E−04
2.55 (−)

5p
GAGGTTTA (132)
TGAGGTTTAAAAATACATAGGCACATGCAAA

TGTGTGTATTATAGTTTTTAATCCTCGGGATA

CAGATTACCTCTTCCTTTTACTGAA (239

bra-miR160a-3p
GCGTATGAGGAG
TATGTGTAGTTGTATAAGATGTGTATGCCTGG
1.90E−04
1.94 (−)

CCATGCATA (133)
CTCCCTGTATGCCATCCTCTAAGCTCATCGAC

CATTGATGACCTCCGTGAATGGCGTATGAGG

AGCCATGCATATTTTCATATACATTTACATAC

(240)

csi-miR162-5p
TGGAGGCAGCGG
AAACTGTTTACACTGATCTGTGCTGCTGATAA
1.60E−03
1.95 (−)

TTCATCGATC
ATCTTAATTTTTTTTTTTGAATTTTTATTTAAC

(134)
AGAAAATAGAGAGAGTGAAGTCACTGGAGG

CAGCGGTTCATCGATCACTTTGTGCAAATTTT

GTTGTGAAAAATAACACAAAATACATGAATC

GATCGATAAACCTCTGCATCCAGCGCTCACT

CCAACTCTATTC (241)

gma-miR1524
CGAGTCCGAGGA
GCGACTTATTGGAGTTCATTCTTCGCACTCTC
3.30E−03
1.73 (−)

AGGAACTCC (135)
TCGGAAACCACTTGTTTCCAATCATCTAATCA

GACGATAGCAGACTCAAGAAAGACGTTTCCT

TCCCAGATCCTTCTAGACCATTTGCAAACCGT

CTCCTTCCCCGAATCCATTCTCCAAACCCTCG

ATCCTTGAGGAGCTCCACCACCGTGACGGCG

CTCCGGTCTCCGCCGTCAATTGTGCCGTCGCG

GTGGAGCATGAGCGTCTTCATGAGTCTGAAA

GGGAATTATAGGAACTACTTTCCTGATTAGG

TTATTGGAAACAAGTGGTTTCCGAGTCCGAG

GAAGGAACTCCAACGCCCAAC (242)

gma-miR159d
AGCTGCTTAGCTA
GGGTGAATTGAGCTGCTTAGCTATGGATCCC
3.20E−06
3.24 (−)

TGGATCCC (136)
ACAGTTCTACCCATCAATAAGTGCTTTTGTGG

TAGTCTTGTGGCTTCCATATCTGGGGAGCTTC

ATTTGCCT (243)

gma-miR2119
TCAAAGGGAGTT
ATACTTCATTTTTTATACTTTAATTTCCTCTAT
2.70E−03
1.54 (−)

GTAGGGGAA (137)
ACCTCACTTTTATTGGAGAAAAAAGAGAATA

GAAAATAGTGGATTTCTCTTCTTTTTTTCAAT

CAAAGGGAGTTGTAGGGGAAAGTTTAGAAA

ATGGCGTGT (244)

gma-miR396d
AAGAAAGCTGTG
GGTCATGCTTTTCCACAGCTTTCTTGAACTTC
4.60E−08
2.76 (−)

GGAGAATATGGC
TTATGCATCTTATATCTCTCCACTTCCAGCAT

(138)
TTTAAGCCCTAGAAGCTCAAGAAAGCTGTGG

GAGAATATGGCAA (245)

gma-miR4412-
AGTGGCGTAGAT
AACTGTTGCGGGTATCTTTGCCTCTGAAGGA
9.80E−05
3.03 (−)

3p
CCCCACAAC (139)
AAGTTGTGCCTATTATTATGGCTTATTGCTTT

AGTGGCGTAGATCCCCACAACAGTT (246)

gma-miR4416a
ACGGGTCGCTCTC
CTTTGATCTGGGTGAGAGAAACGCGTATCGA
9.20E−06
4.30 (−)

ACCTAGG (140)
TGGATTGGGTTCAGTTCTGGTCTCACACGGTT

TGTTCTAACAATTTGTACTGACTGTGTTTTGA

TCGATACGGGTCGCTCTCACCTAGGCCAGAG

TTGC (247)

gma-miR482b-
TATGGGGGGATT
GGTATGGGGGGATTGGGAAGGAATATCCATA
2.30E−10
2.74 (−)

5p
GGGAAGGAAT (141)
AGCAAAATATGCTATTTCTTCCCTACACCTCC

CATACC (248)

osa-miR162a
TCGATAAACCTCT
GGTGATGCCTGGGCGCAGTGGTTTATCGATC
4.00E−04
1.52 (−)

GCATCCAG (142)
CCTTCCCTGCCTTGTGGCGCTGATCCAGGAGC

GGCGAATTTCTTTGAGAGGGTGTTCTTTTTTT

TTCTTCCTTTTGGTCCTTGTTGCAGCCAACGA

CAACGCGGGAATCGATCGATAAACCTCTGCA

TCCAGTTCTCGCC (249)

osa-miR1846e
CAACGAGGAGGC
CGCATCCGCCAACGAGGAGGCCGGGACCACC
4.30E−04
1.90 (−)

CGGGACCA (143)
GGATCCGGTGACTCCGGCCTCCTCGCCGGCA

GATCCGG (250)

osa-miR2104
GCGGCGAGGGGA
ACGGGCGCTCACGGTGGCTTCGACCCTCGTC
3.90E−02
1.51 (−)

TGCGAGCGTG (144)
TCGGCCGCGTGCGGTAGTGCGGGAGGCATGC

CGTGTGTACCGGCGGCGAGGGGATGCGAGCG

TGAGTGCCTCGG (251)

osa-miRf10849-
TGGACTGTTTGGG
GCTGGACTGTTTGGGGGAGCTTCTGATTTTGG
1.80E−03
1.52 (−)

akr
GGAGCTTCT (145)
GAGAAACGGCTATAGCTAGAAGCTCCCCGAA

ACAGGCCCAAC (11943)

osa-miRf11415-
GAGAGCAGGATG
GAAGAGGCAGAGAGCAGGATGCAGCCAAGG
6.70E−03
1.55 (−)

akr
CAGCCAAGG (146)
ATGACTTGCCGGCCGGCGATGGCCGACGGCG

AGGTTAATTAATTGGCCGGAGACTGGCAGTC

CTTCTCTGTTGATCCGGCAAGTTTGTCCTTGG

CTACACCTTGCTCTCTTCTCGTC (11944)

osa-miRf11996-
GTCTTATAACCTG
TACTACCTCCATTTCAGGTTATAAGACTTTCT
7.20E−03
2.01 (−)

akr
AAACGGGGG (147)
AGTGTTGCTCACATTCATATATATGTTAATAA

ATTCATTAACATATAGAAAGTCTTATAACCT

GAAACGGGGGAAGTA (11945)

ppt-miR533b-5p
GAGCTGTCCAGG
GGAGGACCGATATGGAGAGCTGTCCAGGCTG
7.90E−04
3.23 (−)

CTGTGAGGG (148)
TGAGGGGAGCACTCGTATTCTTTTGACCTTTG

CTAGAAGAGGGAATACAGCGCTCTCCCTCAC

AGTCTGTACAGCTCTCTGTATCTCTTCCTCT

(252)

ptc-miRf10007-
CATTGACAGGGA
TTGCTGTGGTGAGTTTCCCTGTCAGTGCTCAC
3.90E−02
1.77 (−)

akr
AACTCACCA (149)
TACGATATTTAATGAAGAAGAAAAATAAAGC

AAGAGATAAAAAAGGCATTTCCTCGATTCAG

ATTTCAGGGTGCAGCATTGCATTGAGCATTG

ACAGGGAAACTCACCACGGCAA (11946)

ptc-miRf10976-
TGGGAACGTGGC
GCCCTGTTTGGGAACGTGGCTGTGGCTACAC
2.30E−04
1.81 (−)

akr
TGTGGCTA (150)
TGATGCTTCTGGTTTGGAAATGGAGGTGCAA

CTGAAGTTATGGGAACGTTCCCAAACAGGGC

(11947)

ptc-miRf11018-
CTGCAAACCTAA
CTGCAAACCTAAGGGAGCGGTTTTGCAGACC
1.10E−02
1.79 (−)

akr
GGGAGCGG (151)
CCAAGCGCACAAGTCTGCAGACCCGCTCGCT

TGGGTCTGCAG (11948)

ptc-miRf11079-
AAGATGGAGAAG
GTGTGTGAGCAAGATGGAGAAGCAGGGCAC
2.30E−05
2.43 (−)

akr
CAGGGCACGTGC
GTGCACTACTAACTCATGCACACAGAGAGGG

(152)
AGACGCATTTCTTGCTGGAGTTACGAGTTAC

GACTCTTACCTACTATTGATTTTGTTAGCTCC

AGTGAGTTAGTTATTCATGTGCCTGTCTTCCT

CATCATGATCACTAC (11949)

ptc-miRf11324-
CTTGTCGCAGGA
TTGGGGGTTTCTTGTCGCAGGAGAGATGGCG
5.90E−05
1.73 (−)

akr
GAGATGGCGCT
CTAGCTAACCATGGTCATATCATATATATCAT

(153)
ATGGCAAGTATTACTTGCTCTTTGTATGTATC

AGCTGTAAAGATAGCTCAGCTAAAGCCATCC

TCCTGCGACTGGACACCCTGCAA (11950)

ptc-miRf11396-
CAAGGCTCTGAT
CTTGGTCATCAAGGCTCTGATACCATGTCAA
4.10E−04
1.51 (−)

akr
ACCATGTCAA (154)
AGAATCATATTTTGAGACCTTATCTAACAGCT

TAAGCTATTGGGTTGAGATGGTTCCTTGACAT

GATATCAGAGCCTTGATGACGAAG (11951)

ptc-miRf11953-
GTAATCTGCATCC
AGGATTGGGTAATCTGCATCCTGAGGTTTGG
1.90E−04
2.74 (−)

akr
TGAGGTT (155)
ATCACCACATGTTTTGATCTAGTCCTTGGGTT

GCAGATTACCTCTTCCT (11952)

ptc-miRf12069-
GGAGGGGCTGCA
CTTGGGCCAGGAGGGGCTGCAAGACCCAAGT
3.90E−07
3.81 (−)

akr
AGACCCAAG (156)
GACTTGGGTCTGCGCTCTTGCCACACCCAAG

CAACTTGGGTCAGACGCCCTTCCAAGCCCCA

AG (11953)

ptc-miRf12389-
GTCGACCTGGCG
TGAGTCGACCTGGCGAGTCAACCGGGTTTGA
1.00E−02
1.75 (−)

akr
AGTCAACCGGG
TTGTTTTTTTATCCTTGCTAGTCTTTCACCTTA

(157)
CTAGGACCGGTCCAGCCACCGGGTTAATCGA

GTCCCGGGTTGACTTGCTGGGCCGTCTGG

(11954)

vvi-miR2111-5p
TAATCTGCATCCT
GCAATATTGGGTCAGGATCGGGTAATCTGCA
3.10E−04
2.82 (−)

GAGGTCTA (158)
TCCTGAGGTCTAGATAAGTATATCTCCGTTGC

AGCTAGTCCTCTGGTTGCAGATTACTTCTTCC

TCACTGCCAATGC (253)

zma-miR167u
TGAAGCTGCCAC
TGAAGCTGCCACATGATCTGATGACGCAGAG
2.20E−05
1.57 (−)

ATGATCTG (159)
TCATGCATATGCATTGCATCCAGCAAGCTCC

ATGCGTGCGTGCATGGCCGAATGGCCGAAGA

GACTAGCTAGTCCATCTCTCCAAGGCCATCC

ACGTGTGAGAATTCAATTCCTCGTGGATCAG

ATCAGGCTGTTGTTGACAACTGCATGCCGCA

CCTGCACTACAGCAACCCAAGGCATAGGTAG

CTAGCTAGGTTTCGGTGGTCAGATCAGATCA

GGCTGGCAGCTTCA (254)

zma-miR396b-
GTTCAATAAAGCT
AGATGGCCTTCTTTGTGATCTTCCACAGCTTT
7.90E−09
6.05 (−)

3p
GTGGGAAA (160)
CTTGAACTGCATCTCTCAGAGGAGCGGCAGC

TTCAACTCCTCCACCCGCATCAGCAGGTGCA

TGCAGTTCAATAAAGCTGTGGGAAACTGCAG

AGAGAGGCCAG (255)

zma-miR398a-
GGGGCGAACTGA
GGGGGCGAACUGAGAACACAUGAGAAUAAU
3.00E−04
1.89 (−)

5p
GAACACATG (161)
GAGAUGAGAUUGCUCGCCUCGCGGUACGGU

UCGUGCUGGCCUGGACCACCGUCGUCGCCG

UUCAUCUUGUACGCAUAAUAAUGCUGCAUG

UGUUCUCAGGUCGCCCCCGC (11955)

TABLE 5

Differentially Expressed (Up-regulated) Small RNAs in Soybean Plants

Growing under Heat Shock (1 hour) versus Optimal Conditions.

Mature Sequence
Stem Loop

Fold-

miR Name
(SEQ ID NO:)
Sequence (SEQ ID NO:)
p-Value
Change

aly-miR831-
AGAAGAGGTACAAGG
AAGTGCTACAAGAATGTATAGTCTTAGAG
4.20E−05
1.73 (+)

5p
AGATGAGA (162)
TCTCAAGAAGAGGTACAAGGAGATGAGA

AGTGAATCACTGAAACAAGTGGTTCTGGT

TTGTGGATCAGTATGGTTTACCCAAAACA

CGTGTTTGGTGCTTCACTTCTAAACTCCTC

GTACTCTTCTTGGGATTCTATGACTTACAC

TTGTTGATTT (256)

ath-
TTAGCTGAAGAAGCA
TTTGTTTGTTTAGCTGAAGAAGCAGAGGA
2.10E−05
1.76 (+)

miRf10687-
GAGGAG (163)
GTCGGCATTGGGGCACAGTCACTCATCGA

akr

TGCTGCAATGGGTAAGTCCTCTGCATACTT

TTGCTGAGATAGGAATAGA (11956)

ath-
GAGGTTTGCGATGAG
GATGTTGGAGGTTTGCGATGAGAAAGAGA
2.10E−07
1.82 (+)

miRf11021-
AAAGAG (164)
TTGGCCGGAAGAATTATCAGCCATCAACA

akr

TCGAGATTGTGAGATAATCGGAAGACCTG

TAATTGTGAAGGTAACTCTTTCTCATCTGC

AAATCTCAACTGTC (11957)

far-miR1134
CGACAACAACAACAA
ACGGCAATCCCAGCTTCAACGGGCCGGTG
2.80E−04
1.63 (+)

GAAGAAGAG (165)
CCAGGCGTGCCTCCCGGCGATGCCCATCG

GTCGCCGACGCCTCCTAGCACGCCAGCTG

GCTCACAAGGTGTCTCTCCCGGCGGCGAC

AACAACAACAAGAAGAAGAGATCAGGTC

TGGTGCTGGCTACTACCATCCCGGTCTCA

GTCAGTGTGGTGGCGCTCATCTCGCTGGG

TGCCGTGCTGCTCTTCCGCAAGAAAAACA

ACGGGTCCG (257)

osa-
TTGGCCTCGTCGAAGA
TCTCGTTCTTGGAGAGGCCCTTGCCGACCT
1.90E−06
2.01 (+)

miRf10105-
AGGAGA (166)
TGGCGATGCGCTTGCCGGCCCTGGACCAG

akr

CGGGACGCCGCGGTCTCCTGCTTGGCCTC

GTCGAAGAAGGAGA (11958)

pab-
TGGCGCTAGAAGGAG
AAATGGCGCTAGAAGGAGGGCCTGAAAA
6.60E−05
1.68 (+)

miR3711
GGCCT (167)
TTATTAATGGCACGAGGCAGTCGTAAGAC

TCCTCCACCACCCAACCACTCACCTATAGT

GAAAAGAAGTCATTAAAATGATAACATCA

CCCCTCAAATAGAACCA (258)

zma-
TGGGAGATGAAGGAG
AGTGGGAGATGAAGGAGCCTTGCATCGAT
8.20E−07
2.05 (+)

miR482-5p
CCTT (168)
GTCACCGCCGGAGGAGCGCTCGCCTTCTT

CGCGCACCGCCGCAATAGCCGCCCTCGGA

CCCCTCGCCTCGCTCTTCCTTGTTCCTCCC

ATTTT (259)

TABLE 6

Differentially Expressed (Down-regulated) Small RNAs in Soybean Plants

Growing under Heat Shock (1 hour) versus Optimal Conditions.

Mature Sequence
Stem Loop

Fold-

miR Name
(SEQ ID NO:)
Sequence (SEQ ID NO:)
p-Value
Change

ath-miRf10279-
ACTCAGCCTGGGG
TGATGGTGATACTCGACATCCAGGTAGA
1.30E−02
2.56 (−)

akr
GTCGAGTGAT
GTGATGAGGTCGAGTAGAGGTCTGGCAA

(169)
TGGGATGAAGTCACTCAGCCTGGGGGTC

GAGTGATGTGATCG (11959)

csi-miR162-5p
TGGAGGCAGCGGT
AAACTGTTTACACTGATCTGTGCTGCTG
4.10E−05
1.71 (−)

TCATCGATC (170)
ATAAATCTTAATTTTTTTTTTTGAATTTTT

ATTTAACAGAAAATAGAGAGAGTGAAG

TCACTGGAGGCAGCGGTTCATCGATCAC

TTTGTGCAAATTTTGTTGTGAAAAATAA

CACAAAATACATGAATCGATCGATAAAC

CTCTGCATCCAGCGCTCACTCCAACTCTA

TTC (260)

gma-miR4412-
AGTGGCGTAGATC
AACTGTTGCGGGTATCTTTGCCTCTGAA
1.60E−05
1.81 (−)

3p
CCCACAAC (171)
GGAAAGTTGTGCCTATTATTATGGCTTAT

TGCTTTAGTGGCGTAGATCCCCACAACA

GTT (261)

osa-miRf10151-
TGGCTATATTTTGG
GTATACTACCTCCGTCCCAAAATATAGC
3.60E−03
2.06 (−)

akr
GACGGAG (172)
CACTTTTAGATTCATAAACAAAAGTGGC

TATATTTTGGGACGGAGGGAGTATAT

(11960)

ptc-miRf12069-
GGAGGGGCTGCAA
CTTGGGCCAGGAGGGGCTGCAAGACCCA
1.90E−03
2.03 (−)

akr
GACCCAAG (173)
AGTGACTTGGGTCTGCGCTCTTGCCACA

CCCAAGCAACTTGGGTCAGACGCCCTTC

CAAGCCCCAAG (11961)

Example 2
Identification of Homologous and Orthologous Sequences of Differential Small RNAs Associated with Enhanced Abiotic Stress Tolerance

The miRNA sequences of the invention that were either down- or up-regulated under abiotic stress conditions were examined for homologous and orthologous sequences using the miRBase database (http://wwwDOTmirbaseDOTorg/) and the Plant MicroRNA Database (PMRD, http://bioinformaticsDOTcauDOTeduDOTcn/PMRD). The mature miRNA sequences that are homologous or orthologous to the soy miRNAs listed in Tables 1-6 above, were found using miRNA public databases, having at least 75% identity of the entire mature miRNA length of the original soy sequence listed in Tables 1-6 and are summarized in Tables 7-8 below.

TABLE 7

Summary of Homologs (Orthologs to Small RNAs which are up-regulated

in Abiotic Stress in Soybean Plants.

Homolog stem-

Homolog Sequence
%
loop sequence

Mir Name
Homolog Name
(SEQ ID NO:)
Identity
(SEQ ID NO:)

far-
tae-miR1134
CAACAACAACAAGAAG
0.88
2087

miR1134

AAGAAGAT (262)

mtr-
gma-miR2119
TCAAAGGGAGTTGTAG
0.76
2088

miR2119

GGGAA (263)

pvu-miR2119
TCAAAGGGAGTTGTAG
0.76
2089

GGGAA (264)

ppt-
ppt-miR1220b
TTCCGGTGGTGAGGAAG
1
2090

miR1220a

ATAG (265)

aqc-
acb-miR159
TTGGACTGAAGGGAGCT
0.86
2091

miR159

CCCT (266)

aha-miR159
TTGGACTGAAGGGAGCT
0.86
2092

CCCT (267)

ahi-miR159
TTGGACTGAAGGGAGCT
0.86
2093

CCCT (268)

ahy-miR159
TTTGGATTGAAGGGAGC
0.95
2094

TCTA (269)

aly-miR159a
TTTGGATTGAAGGGAGC
0.95
2095

TCTA (270)

aly-miR159b
TTTGGATTGAAGGGAGC
0.9
2096

TCTT (271)

aly-miR159c
TTTGGATTGAAGGGAGC
0.86
2097

TCCT (272)

ape-miR159
TTGGACTGAAGGGAGCT
0.86
2098

CCCT (273)

ath-miR159a
TTTGGATTGAAGGGAGC
0.95
2099

TCTA (274)

ath-miR159b
TTTGGATTGAAGGGAGC
0.9
2100

TCTT (275)

ath-miR159c
TTTGGATTGAAGGGAGC
0.86
2101

TCCT (276)

bdi-miR159
CTTGGATTGAAGGGAGC
0.86
2102

TCT (277)

bna-miR159
TTTGGATTGAAGGGAGC
0.95
2103

TCTA (278)

bra-miR159a
TTTGGATTGAAGGGAGC
0.95
2104

TCTA (279)

bvl-miR159
TTGGACTGAAGGGAGCT
0.86
2105

CCCT (280)

cmi-miR159
TTGGACTGAAGGGAGCT
0.86
2106

CCCT (281)

cor-miR159
TTGGACTGAAGGGAGCT
0.86
2107

CCCT (282)

crb-miR159
TTGGACTGAAGGGAGCT
0.86
2108

CCCT (283)

csi-miR159
TTTGGATTGAAGGGAGC
0.95
2109

TCTA (284)

dso-miR159
TTGGACTGAAGGGAGCT
0.86
2110

CCCT (285)

ech-miR159
TTGGACTGAAGGGAGCT
0.86
2111

CCCT (286)

fal-miR159
TTGGACTGAAGGGAGCT
0.86
2112

CCCT (287)

far-miR159
TTTGGATTGAAGGGAGC
0.9
2113

TCTG (288)

gma-miR159a-3p
TTTGGATTGAAGGGAGC
0.95
2114

TCTA (289)

gma-miR159b
ATTGGAGTGAAGGGAG
0.86
2115

CTCCA (290)

gma-miR159c
ATTGGAGTGAAGGGAG
0.81
2116

CTCCG (291)

hvu-miR159a
TTTGGATTGAAGGGAGC
0.9
2117

TCTG (292)

hvu-miR159b
TTTGGATTGAAGGGAGC
0.9
2118

TCTG (293)

hvv-miR159a
TTTGGATTGAAGGGAGC
0.9
2119

TCTG (294)

hvv-miR159b
TTTGGATTGAAGGGAGC
0.9
2120

TCTG (295)

ltu-miR159
TTTGGATTGAAGGGAGC
0.95
2121

TCTA (296)

mma-miR159
TTGGACTGAAGGGAGCT
0.86
2122

CCCT (297)

mtr-miR159a
TTTGGATTGAAGGGAGC
0.95
2123

TCTA (298)

mtr-miR159b
ATTGAATTGAAGGGAG
0.67
2124

CAACT (299)

mtr-miR159c
TTTGGATTGAAGGGAGC
0.95
2125

TCTA (300)

nof-miR159
TTGGACTGAAGGGAGCT
0.86
2126

CCCT (301)

oru-miR159
TTTGGATTGAAGGGAGC
0.9
2127

TCTG (302)

osa-miR159a
TTTGGATTGAAGGGAGC
0.9
2128

TCTG (303)

osa-miR159a.1
TTTGGATTGAAGGGAGC
0.9
2129

TCTG (304)

osa-miR159b
TTTGGATTGAAGGGAGC
0.9
2130

TCTG (305)

osa-miR159c
ATTGGATTGAAGGGAG
0.86
2131

CTCCA (306)

osa-miR159d
ATTGGATTGAAGGGAG
0.81
2132

CTCCG (307)

osa-miR159e
ATTGGATTGAAGGGAG
0.81
2133

CTCCT (308)

osa-miR159f
CTTGGATTGAAGGGAGC
0.9
2134

TCTA (309)

osa-miR159m
TTTGGATTGAAGGGAGC
0.9
2135

TCTG (310)

pgl-miR159
TTTGGATTGAAGGGAGC
0.9
2136

TCTG (311)

psi-miR159
CTTGGATTGAAGGGAGC
0.86
2137

TCCA (312)

pta-miR159a
TTGGATTGAAGGGAGCT
0.86
2138

CCA (313)

pta-miR159b
TTGGATTGAAGAGAGCT
0.76
2139

CCC (314)

pta-miR159c
CTTGGATTGAAGGGAGC
0.81
2140

TCCC (315)

ptc-miR159a
TTTGGATTGAAGGGAGC
0.95
2141

TCTA (316)

ptc-miR159b
TTTGGATTGAAGGGAGC
0.95
2142

TCTA (317)

ptc-miR159c
TTTGGATTGAAGGGAGC
0.95
2143

TCTA (318)

ptc-miR159d
CTTGGATTGAAGGGAGC
0.81
2144

TCCT (319)

ptc-miR159e
CTTGGGGTGAAGGGAG
0.76
2145

CTCCT (320)

ptc-miR159f
ATTGGAGTGAAGGGAG
0.86
2146

CTCGA (321)

pvu-miR159
TTTGGATTGAAGGGAGC
0.95
2147

TCTA (322)

pvu-miR159a.1
TTTGGATTGAAGGGAGC
0.95
2148

TCTA (323)

rco-miR159
TTTGGATTGAAGGGAGC
0.95
2149

TCTA (324)

rin-miR159
TTGGACTGAAGGGAGCT
0.86
2150

CCCT (325)

sar-miR159
TTTGGATTGAAGGGAGC
0.9
2151

TCTG (326)

sbi-miR159a
TTTGGATTGAAGGGAGC
0.9
2152

TCTG (327)

sbi-miR159b
CTTGGATTGAAGGGAGC
0.81
2153

TCCT (328)

sly-miR159
TTTGGATTGAAGGGAGC
0.95
2154

TCTA (329)

smo-miR159
CTTGGATTGAAGGGAGC
0.81
2155

TCCC (330)

sof-miR159a
TTTGGATTGAAGGGAGC
0.9
2156

TCTG (331)

sof-miR159b
TTTGGATTGAAGGGAGC
0.9
2157

TCTG (332)

sof-miR159c
CTTGGATTGAAGGGAGC
0.81
2158

TCCT (333)

sof-miR159d
TTTGGATTGAAGGGAGC
0.9
2159

TCTG (334)

sof-miR159e
TTTGGATTGAAAGGAGC
0.86
2160

TCTT (335)

spr-miR159
TTTGGATTGAAGGGAGC
0.9
2161

TCTG (336)

ssp-miR159a
TTTGGATTGAAGGGAGC
0.9
2162

TCTG (337)

svi-miR159
TTGGACTGAAGGGAGCT
0.86
2163

CCCT (338)

tae-miR159a
TTTGGATTGAAGGGAGC
0.9
2164

TCTG (339)

tae-miR159b
TTTGGATTGAAGGGAGC
0.9
2165

TCTG (340)

tar-miR159
TTGGACTGAAGGGAGCT
0.86
2166

CCCT (341)

vvi-miR159a
CTTGGAGTGAAGGGAG
0.86
2167

CTCTC (342)

vvi-miR159b
CTTGGAGTGAAGGGAG
0.86
2168

CTCTC (343)

vvi-miR159c
TTTGGATTGAAGGGAGC
0.95
2169

TCTA (344)

zma-miR159a
TTTGGATTGAAGGGAGC
0.9
2170

TCTG (345)

zma-miR159b
TTTGGATTGAAGGGAGC
0.9
2171

TCTG (346)

zma-miR159c
CTTGGATTGAAGGGAGC
0.81
2172

TCCT (347)

zma-miR159d
CTTGGATTGAAGGGAGC
0.81
2173

TCCT (348)

zma-miR159e
ATTGGTTTGAAGGGAGC
0.81
2174

TCCA (349)

zma-miR159f
TTTGGATTGAAGGGAGC
0.9
2175

TCTG (350)

zma-miR159g
TTTGGAGTGAAGGGAGT
0.86
2176

TCTG (351)

zma-miR159h
TTTGGAGTGAAGGGAG
0.9
2177

CTCTG (352)

zma-miR159i
TTTGGAGTGAAGGGAG
0.9
2178

CTCTG (353)

zma-miR159j
TTTGGATTGAAGGGAGC
0.9
2179

TCTG (354)

zma-miR159k
TTTGGATTGAAGGGAGC
0.9
2180

TCTG (355)

zma-miR159m
TTTGGATTGAAGGGAGC
0.9
2181

TCTG (356)

ath-
acb-miR159
TTGGACTGAAGGGAGCT
0.81
2182

miR159b

CCCT (357)

aha-miR159
TTGGACTGAAGGGAGCT
0.81
2183

CCCT (358)

ahi-miR159
TTGGACTGAAGGGAGCT
0.81
2184

CCCT (359)

ahy-miR159
TTTGGATTGAAGGGAGC
0.95
2185

TCTA (360)

aly-miR159a
TTTGGATTGAAGGGAGC
0.95
2186

TCTA (361)

aly-miR159b
TTTGGATTGAAGGGAGC
1
2187

TCTT (362)

aly-miR159c
TTTGGATTGAAGGGAGC
0.95
2188

TCCT (363)

ape-miR159
TTGGACTGAAGGGAGCT
0.81
2189

CCCT (364)

aqc-miR159
TTTGGACTGAAGGGAGC
0.9
2190

TCTA (365)

ath-miR159a
TTTGGATTGAAGGGAGC
0.95
2191

TCTA (366)

ath-miR159c
TTTGGATTGAAGGGAGC
0.95
2192

TCCT (367)

bdi-miR159
CTTGGATTGAAGGGAGC
0.9
2193

TCT (368)

bna-miR159
TTTGGATTGAAGGGAGC
0.95
2194

TCTA (369)

bra-miR159a
TTTGGATTGAAGGGAGC
0.95
2195

TCTA (370)

byl-miR159
TTGGACTGAAGGGAGCT
0.81
2196

CCCT (371)

cmi-miR159
TTGGACTGAAGGGAGCT
0.81
2197

CCCT (372)

cor-miR159
TTGGACTGAAGGGAGCT
0.81
2198

CCCT (373)

crb-miR159
TTGGACTGAAGGGAGCT
0.81
2199

CCCT (374)

csi-miR159
TTTGGATTGAAGGGAGC
0.95
2200

TCTA (375)

dso-miR159
TTGGACTGAAGGGAGCT
0.81
2201

CCCT (376)

ech-miR159
TTGGACTGAAGGGAGCT
0.81
2202

CCCT (377)

fal-miR159
TTGGACTGAAGGGAGCT
0.81
2203

CCCT (378)

far-miR159
TTTGGATTGAAGGGAGC
0.95
2204

TCTG (379)

gma-miR159a-3p
TTTGGATTGAAGGGAGC
0.95
2205

TCTA (380)

gma-miR159b
ATTGGAGTGAAGGGAG
0.81
2206

CTCCA (381)

gma-miR159c
ATTGGAGTGAAGGGAG
0.81
2207

CTCCG (382)

hvu-miR159a
TTTGGATTGAAGGGAGC
0.95
2208

TCTG (383)

hvu-miR159b
TTTGGATTGAAGGGAGC
0.95
2209

TCTG (384)

hvv-miR159a
TTTGGATTGAAGGGAGC
0.95
2210

TCTG (385)

hvv-miR159b
TTTGGATTGAAGGGAGC
0.95
2211

TCTG (386)

ltu-miR159
TTTGGATTGAAGGGAGC
0.95
2212

TCTA (387)

mma-miR159
TTGGACTGAAGGGAGCT
0.81
2213

CCCT (388)

mtr-miR159a
TTTGGATTGAAGGGAGC
0.95
2214

TCTA (389)

mtr-miR159b
ATTGAATTGAAGGGAG
0.76
2215

CAACT (390)

mtr-miR159c
TTTGGATTGAAGGGAGC
0.95
2216

TCTA (391)

nof-miR159
TTGGACTGAAGGGAGCT
0.81
2217

CCCT (392)

oru-miR159
TTTGGATTGAAGGGAGC
0.95
2218

TCTG (393)

osa-miR159a
TTTGGATTGAAGGGAGC
0.95
2219

TCTG (394)

osa-miR159a.1
TTTGGATTGAAGGGAGC
0.95
2220

TCTG (395)

osa-miR159b
TTTGGATTGAAGGGAGC
0.95
2221

TCTG (396)

osa-miR159c
ATTGGATTGAAGGGAG
0.86
2222

CTCCA (397)

osa-miR159d
ATTGGATTGAAGGGAG
0.86
2223

CTCCG (398)

osa-miR159e
ATTGGATTGAAGGGAG
0.9
2224

CTCCT (399)

osa-miR159f
CTTGGATTGAAGGGAGC
0.9
2225

TCTA (400)

osa-miR159m
TTTGGATTGAAGGGAGC
0.95
2226

TCTG (401)

pgl-miR159
TTTGGATTGAAGGGAGC
0.95
2227

TCTG (402)

psi-miR159
CTTGGATTGAAGGGAGC
0.86
2228

TCCA (403)

pta-miR159a
TTGGATTGAAGGGAGCT
0.86
2229

CCA (404)

pta-miR159b
TTGGATTGAAGAGAGCT
0.81
2230

CCC (405)

pta-miR159c
CTTGGATTGAAGGGAGC
0.86
2231

TCCC (406)

ptc-miR159a
TTTGGATTGAAGGGAGC
0.95
2232

TCTA (407)

ptc-miR159b
TTTGGATTGAAGGGAGC
0.95
2233

TCTA (408)

ptc-miR159c
TTTGGATTGAAGGGAGC
0.95
2234

TCTA (409)

ptc-miR159d
CTTGGATTGAAGGGAGC
0.9
2235

TCCT (410)

ptc-miR159e
CTTGGGGTGAAGGGAG
0.81
2236

CTCCT (411)

ptc-miR159f
ATTGGAGTGAAGGGAG
0.81
2237

CTCGA (412)

pvu-miR159
TTTGGATTGAAGGGAGC
0.95
2238

TCTA (413)

pvu-miR159a.1
TTTGGATTGAAGGGAGC
0.95
2239

TCTA (414)

rco-miR159
TTTGGATTGAAGGGAGC
0.95
2240

TCTA (415)

rin-miR159
TTGGACTGAAGGGAGCT
0.81
2241

CCCT (416)

sar-miR159
TTTGGATTGAAGGGAGC
0.95
2242

TCTG (417)

sbi-miR159a
TTTGGATTGAAGGGAGC
0.95
2243

TCTG (418)

sbi-miR159b
CTTGGATTGAAGGGAGC
0.9
2244

TCCT (419)

sly-miR159
TTTGGATTGAAGGGAGC
0.95
2245

TCTA (420)

smo-miR159
CTTGGATTGAAGGGAGC
0.86
2246

TCCC (421)

sof-miR159a
TTTGGATTGAAGGGAGC
0.95
2247

TCTG (422)

sof-miR159b
TTTGGATTGAAGGGAGC
0.95
2248

TCTG (423)

sof-miR159c
CTTGGATTGAAGGGAGC
0.9
2249

TCCT (424)

sof-miR159d
TTTGGATTGAAGGGAGC
0.95
2250

TCTG (425)

sof-miR159e
TTTGGATTGAAAGGAGC
0.95
2251

TCTT (426)

spr-miR159
TTTGGATTGAAGGGAGC
0.95
2252

TCTG (427)

ssp-miR159a
TTTGGATTGAAGGGAGC
0.95
2253

TCTG (428)

svi-miR159
TTGGACTGAAGGGAGCT
0.81
2254

CCCT (429)

tae-miR159a
TTTGGATTGAAGGGAGC
0.95
2255

TCTG (430)

tae-miR159b
TTTGGATTGAAGGGAGC
0.95
2256

TCTG (431)

tar-miR159
TTGGACTGAAGGGAGCT
0.81
2257

CCCT (432)

vvi-miR159a
CTTGGAGTGAAGGGAG
0.86
2258

CTCTC (433)

vvi-miR159b
CTTGGAGTGAAGGGAG
0.86
2259

CTCTC (434)

vvi-miR159c
TTTGGATTGAAGGGAGC
0.95
2260

TCTA (435)

zma-miR159a
TTTGGATTGAAGGGAGC
0.95
2261

TCTG (436)

zma-miR159b
TTTGGATTGAAGGGAGC
0.95
2262

TCTG (437)

zma-miR159c
CTTGGATTGAAGGGAGC
0.9
2263

TCCT (438)

zma-miR159d
CTTGGATTGAAGGGAGC
0.9
2264

TCCT (439)

zma-miR159e
ATTGGTTTGAAGGGAGC
0.81
2265

TCCA (440)

zma-miR159f
TTTGGATTGAAGGGAGC
0.95
2266

TCTG (441)

zma-miR159g
TTTGGAGTGAAGGGAGT
0.86
2267

TCTG (442)

zma-miR159h
TTTGGAGTGAAGGGAG
0.9
2268

CTCTG (443)

zma-miR159i
TTTGGAGTGAAGGGAG
0.9
2269

CTCTG (444)

zma-miR159j
TTTGGATTGAAGGGAGC
0.95
2270

TCTG (445)

zma-miR159k
TTTGGATTGAAGGGAGC
0.95
2271

TCTG (446)

zma-miR159m
TTTGGATTGAAGGGAGC
0.95
2272

TCTG (447)

ath-
acb-miR159
TTGGACTGAAGGGAGCT
0.86
2273

miR159c

CCCT (448)

aha-miR159
TTGGACTGAAGGGAGCT
0.86
2274

CCCT (449)

ahi-miR159
TTGGACTGAAGGGAGCT
0.86
2275

CCCT (450)

ahy-miR159
TTTGGATTGAAGGGAGC
0.9
2276

TCTA (451)

aly-miR159a
TTTGGATTGAAGGGAGC
0.9
2277

TCTA (452)

aly-miR159b
TTTGGATTGAAGGGAGC
0.95
2278

TCTT (453)

aly-miR159c
TTTGGATTGAAGGGAGC
1
2279

TCCT (454)

ape-miR159
TTGGACTGAAGGGAGCT
0.86
2280

CCCT (455)

aqc-miR159
TTTGGACTGAAGGGAGC
0.86
2281

TCTA (456)

ath-miR159a
TTTGGATTGAAGGGAGC
0.9
2282

TCTA (457)

ath-miR159b
TTTGGATTGAAGGGAGC
0.95
2283

TCTT (458)

bdi-miR159
CTTGGATTGAAGGGAGC
0.86
2284

TCT (459)

bna-miR159
TTTGGATTGAAGGGAGC
0.9
2285

TCTA (460)

bra-miR159a
TTTGGATTGAAGGGAGC
0.9
2286

TCTA (461)

byl-miR159
TTGGACTGAAGGGAGCT
0.86
2287

CCCT (462)

cmi-miR159
TTGGACTGAAGGGAGCT
0.86
2288

CCCT (463)

cor-miR159
TTGGACTGAAGGGAGCT
0.86
2289

CCCT (464)

crb-miR159
TTGGACTGAAGGGAGCT
0.86
2290

CCCT (465)

csi-miR159
TTTGGATTGAAGGGAGC
0.9
2291

TCTA (466)

dso-miR159
TTGGACTGAAGGGAGCT
0.86
2292

CCCT (467)

ech-miR159
TTGGACTGAAGGGAGCT
0.86
2293

CCCT (468)

fal-miR159
TTGGACTGAAGGGAGCT
0.86
2294

CCCT (469)

far-miR159
TTTGGATTGAAGGGAGC
0.9
2295

TCTG (470)

gma-miR159a-3p
TTTGGATTGAAGGGAGC
0.9
2296

TCTA (471)

gma-miR159b
ATTGGAGTGAAGGGAG
0.86
2297

CTCCA (472)

gma-miR159c
ATTGGAGTGAAGGGAG
0.86
2298

CTCCG (473)

hvu-miR159a
TTTGGATTGAAGGGAGC
0.9
2299

TCTG (474)

hvu-miR159b
TTTGGATTGAAGGGAGC
0.9
2300

TCTG (475)

hvv-miR159a
TTTGGATTGAAGGGAGC
0.9
2301

TCTG (476)

hvv-miR159b
TTTGGATTGAAGGGAGC
0.9
2302

TCTG (477)

ltu-miR159
TTTGGATTGAAGGGAGC
0.9
2303

TCTA (478)

mma-miR159
TTGGACTGAAGGGAGCT
0.86
2304

CCCT (479)

mtr-miR159a
TTTGGATTGAAGGGAGC
0.9
2305

TCTA (480)

mtr-miR159b
ATTGAATTGAAGGGAG
0.81
2306

CAACT (481)

mtr-miR159c
TTTGGATTGAAGGGAGC
0.9
2307

TCTA (482)

nof-miR159
TTGGACTGAAGGGAGCT
0.86
2308

CCCT (483)

oru-miR159
TTTGGATTGAAGGGAGC
0.9
2309

TCTG (484)

osa-miR159a
TTTGGATTGAAGGGAGC
0.9
2310

TCTG (485)

osa-miR159a.1
TTTGGATTGAAGGGAGC
0.9
2311

TCTG (486)

osa-miR159b
TTTGGATTGAAGGGAGC
0.9
2312

TCTG (487)

osa-miR159c
ATTGGATTGAAGGGAG
0.9
2313

CTCCA (488)

osa-miR159d
ATTGGATTGAAGGGAG
0.9
2314

CTCCG (489)

osa-miR159e
ATTGGATTGAAGGGAG
0.95
2315

CTCCT (490)

osa-miR159f
CTTGGATTGAAGGGAGC
0.86
2316

TCTA (491)

osa-miR159m
TTTGGATTGAAGGGAGC
0.9
2317

TCTG (492)

pgl-miR159
TTTGGATTGAAGGGAGC
0.9
2318

TCTG (493)

psi-miR159
CTTGGATTGAAGGGAGC
0.9
2319

TCCA (494)

pta-miR159a
TTGGATTGAAGGGAGCT
0.9
2320

CCA (495)

pta-miR159b
TTGGATTGAAGAGAGCT
0.86
2321

CCC (496)

pta-miR159c
CTTGGATTGAAGGGAGC
0.9
2322

TCCC (497)

ptc-miR159a
TTTGGATTGAAGGGAGC
0.9
2323

TCTA (498)

ptc-miR159b
TTTGGATTGAAGGGAGC
0.9
2324

TCTA (499)

ptc-miR159c
TTTGGATTGAAGGGAGC
0.9
2325

TCTA (500)

ptc-miR159d
CTTGGATTGAAGGGAGC
0.95
2326

TCCT (501)

ptc-miR159e
CTTGGGGTGAAGGGAG
0.86
2327

CTCCT (502)

ptc-miR159f
ATTGGAGTGAAGGGAG
0.81
2328

CTCGA (503)

pvu-miR159
TTTGGATTGAAGGGAGC
0.9
2329

TCTA (504)

pvu-miR159a.1
TTTGGATTGAAGGGAGC
0.9
2330

TCTA (505)

rco-miR159
TTTGGATTGAAGGGAGC
0.9
2331

TCTA (506)

rin-miR159
TTGGACTGAAGGGAGCT
0.86
2332

CCCT (507)

sar-miR159
TTTGGATTGAAGGGAGC
0.9
2333

TCTG (508)

sbi-miR159a
TTTGGATTGAAGGGAGC
0.9
2334

TCTG (509)

sbi-miR159b
CTTGGATTGAAGGGAGC
0.95
2335

TCCT (510)

sly-miR159
TTTGGATTGAAGGGAGC
0.9
2336

TCTA (511)

smo-miR159
CTTGGATTGAAGGGAGC
0.9
2337

TCCC (512)

sof-miR159a
TTTGGATTGAAGGGAGC
0.9
2338

TCTG (513)

sof-miR159b
TTTGGATTGAAGGGAGC
0.9
2339

TCTG (514)

sof-miR159c
CTTGGATTGAAGGGAGC
0.95
2340

TCCT (515)

sof-miR159d
TTTGGATTGAAGGGAGC
0.9
2341

TCTG (516)

sof-miR159e
TTTGGATTGAAAGGAGC
0.9
2342

TCTT (517)

spr-miR159
TTTGGATTGAAGGGAGC
0.9
2343

TCTG (518)

ssp-miR159a
TTTGGATTGAAGGGAGC
0.9
2344

TCTG (519)

svi-miR159
TTGGACTGAAGGGAGCT
0.86
2345

CCCT (520)

tae-miR159a
TTTGGATTGAAGGGAGC
0.9
2346

TCTG (521)

tae-miR159b
TTTGGATTGAAGGGAGC
0.9
2347

TCTG (522)

tar-miR159
TTGGACTGAAGGGAGCT
0.86
2348

CCCT (523)

vvi-miR159a
CTTGGAGTGAAGGGAG
0.81
2349

CTCTC (524)

vvi-miR159b
CTTGGAGTGAAGGGAG
0.81
2350

CTCTC (525)

vvi-miR159c
TTTGGATTGAAGGGAGC
0.9
2351

TCTA (526)

zma-miR159a
TTTGGATTGAAGGGAGC
0.9
2352

TCTG (527)

zma-miR159b
TTTGGATTGAAGGGAGC
0.9
2353

TCTG (528)

zma-miR159c
CTTGGATTGAAGGGAGC
0.95
2354

TCCT (529)

zma-miR159d
CTTGGATTGAAGGGAGC
0.95
2355

TCCT (530)

zma-miR159e
ATTGGTTTGAAGGGAGC
0.86
2356

TCCA (531)

zma-miR159f
TTTGGATTGAAGGGAGC
0.9
2357

TCTG (532)

zma-miR159g
TTTGGAGTGAAGGGAGT
0.81
2358

TCTG (533)

zma-miR159h
TTTGGAGTGAAGGGAG
0.86
2359

CTCTG (534)

zma-miR159i
TTTGGAGTGAAGGGAG
0.86
2360

CTCTG (535)

zma-miR159j
TTTGGATTGAAGGGAGC
0.9
2361

TCTG (536)

zma-miR159k
TTTGGATTGAAGGGAGC
0.9
2362

TCTG (537)

zma-miR159m
TTTGGATTGAAGGGAGC
0.9
2363

TCTG (538)

ath-
gma-miRf10687-
TTAGCCGCAGAGGCAG
0.86
11615

miRf10687-
akr-homolog
AGGAG (11616)

akr

ghr-
vvi-miR2950*
TGGTGTGCACGGGATGG
0.9
2364

miR2950

AATA (539)

gma-
ahy-miR156a
TGACAGAAGAGAGAGA
0.85
2365

miR156g

GCAC (540)

ahy-miR156b-5p
TTGACAGAAGATAGAG
0.9
2366

AGCAC (541)

ahy-miR156c
TTGACAGAAGAGAGAG
0.85
2367

AGCAC (542)

aly-miR156a
TGACAGAAGAGAGTGA
0.8
2368

GCAC (543)

aly-miR156b
TGACAGAAGAGAGTGA
0.8
2369

GCAC (544)

aly-miR156c
TGACAGAAGAGAGTGA
0.8
2370

GCAC (545)

aly-miR156d
TGACAGAAGAGAGTGA
0.8
2371

GCAC (546)

aly-miR156e
TGACAGAAGAGAGTGA
0.8
2372

GCAC (547)

aly-miR156f
TGACAGAAGAGAGTGA
0.8
2373

GCAC (548)

aly-miR156g
CGACAGAAGAGAGTGA
0.8
2374

GCAC (549)

aly-miR156h
TGACAGAAGAAAGAGA
0.85
2375

GCAC (550)

aqc-miR156a
TGACAGAAGATAGAGA
0.9
2376

GCAC (551)

aqc-miR156b
TGACAGAAGATAGAGA
0.9
2377

GCAC (552)

ath-miR156a
TGACAGAAGAGAGTGA
0.8
2378

GCAC (553)

ath-miR156b
TGACAGAAGAGAGTGA
0.8
2379

GCAC (554)

ath-miR156c
TGACAGAAGAGAGTGA
0.8
2380

GCAC (555)

ath-miR156d
TGACAGAAGAGAGTGA
0.8
2381

GCAC (556)

ath-miR156e
TGACAGAAGAGAGTGA
0.8
2382

GCAC (557)

ath-miR156f
TGACAGAAGAGAGTGA
0.8
2383

GCAC (558)

ath-miR156g
CGACAGAAGAGAGTGA
0.8
2384

GCAC (559)

ath-miR156h
TGACAGAAGAAAGAGA
0.85
2385

GCAC (560)

bdi-miR156
TGACAGAAGAGAGAGA
0.9
2386

GCACA (561)

bdi-miR156b
TGACAGAAGAGAGTGA
0.8
2387

GCAC (562)

bdi-miR156c
TGACAGAAGAGAGTGA
0.8
2388

GCAC (563)

bdi-miR156d
TGACAGAAGAGAGTGA
0.8
2389

GCAC (564)

bna-miR156a
TGACAGAAGAGAGTGA
0.85
2390

GCACA (565)

bna-miR156b
TTGACAGAAGATAGAG
0.9
2391

AGCAC (566)

bna-miR156c
TTGACAGAAGATAGAG
0.9
2392

AGCAC (567)

csi-miR156
TGACAGAAGAGAGTGA
0.8
2393

GCAC (568)

ctr-miR156
TGACAGAAGAGAGTGA
0.8
2394

GCAC (569)

far-miR156a
TGACAGAAGAGAGAGA
0.9
2395

GCACA (570)

far-miR156b
TTGACAGAAGAGAGAG
0.85
2396

AGCAC (571)

ghr-miR156a
TGACAGAAGAGAGTGA
0.8
2397

GCAC (572)

ghr-miR156b
TGACAGAAGAGAGTGA
0.8
2398

GCAC (573)

ghr-miR156c
TGTCAGAAGAGAGTGA
0.75
2399

GCAC (574)

ghr-miR156d
TGACAGAAGAGAGTGA
0.8
2400

GCAC (575)

gma-miR156a
TGACAGAAGAGAGTGA
0.8
2401

GCAC (576)

gma-miR156b
TGACAGAAGAGAGAGA
0.9
2402

GCACA (577)

gma-miR156c
TTGACAGAAGATAGAG
0.9
2403

AGCAC (578)

gma-miR156d
TTGACAGAAGATAGAG
0.9
2404

AGCAC (579)

gma-miR156e
TTGACAGAAGATAGAG
0.9
2405

AGCAC (580)

gma-miR156f
TTGACAGAAGAGAGAG
0.9
2406

AGCACA (581)

hvu-miR156
TGACAGAAGAGAGTGA
0.85
2407

GCACA (582)

mtr-miR156
TGACAGAAGAGAGAGA
0.9
2408

GCACA (583)

mtr-miR156b
TGACAGAAGAGAGTGA
0.8
2409

GCAC (584)

mtr-miR156c
TGACAGAAGAGAGTGA
0.8
2410

GCAC (585)

mtr-miR156d
TGACAGAAGAGAGTGA
0.8
2411

GCAC (586)

mtr-miR156e
TTGACAGAAGATAGAG
0.9
2412

AGCAC (587)

mtr-miR156f
TTGACAGAAGATAGAG
0.9
2413

AGCAC (588)

mtr-miR156g
TTGACAGAAGATAGAG
0.85
2414

GGCAC (589)

mtr-miR156h
TTGACAGAAGATAGAG
0.9
2415

AGCAC (590)

mtr-miR156i
TGACAGAAGAGAGTGA
0.8
2416

GCAC (591)

osa-miR156a
TGACAGAAGAGAGTGA
0.8
2417

GCAC (592)

osa-miR156b
TGACAGAAGAGAGTGA
0.8
2418

GCAC (593)

osa-miR156c
TGACAGAAGAGAGTGA
0.8
2419

GCAC (594)

osa-miR156d
TGACAGAAGAGAGTGA
0.8
2420

GCAC (595)

osa-miR156e
TGACAGAAGAGAGTGA
0.8
2421

GCAC (596)

osa-miR156f
TGACAGAAGAGAGTGA
0.8
2422

GCAC (597)

osa-miR156g
TGACAGAAGAGAGTGA
0.8
2423

GCAC (598)

osa-miR156h
TGACAGAAGAGAGTGA
0.8
2424

GCAC (599)

osa-miR156i
TGACAGAAGAGAGTGA
0.8
2425

GCAC (600)

osa-miR156j
TGACAGAAGAGAGTGA
0.8
2426

GCAC (601)

osa-miR156k
TGACAGAAGAGAGAGA
0.9
2427

GCACA (602)

osa-miR156l
CGACAGAAGAGAGTGA
0.8
2428

GCATA (603)

ppt-miR156a
TGACAGAAGAGAGTGA
0.8
2429

GCAC (604)

ppt-miR156b
TGACAGAAGAGAGTGA
0.8
2430

GCAC (605)

ppt-miR156c
TGACAGAAGAGAGTGA
0.8
2431

GCAC (606)

pta-miR156a
CAGAAGATAGAGAGCA
0.9
2432

CATC (607)

pta-miR156b
CAGAAGATAGAGAGCA
0.9
2433

CAAC (608)

ptc-miR156a
TGACAGAAGAGAGTGA
0.8
2434

GCAC (609)

ptc-miR156b
TGACAGAAGAGAGTGA
0.8
2435

GCAC (610)

ptc-miR156c
TGACAGAAGAGAGTGA
0.8
2436

GCAC (611)

ptc-miR156d
TGACAGAAGAGAGTGA
0.8
2437

GCAC (612)

ptc-miR156e
TGACAGAAGAGAGTGA
0.8
2438

GCAC (613)

ptc-miR156f
TGACAGAAGAGAGTGA
0.8
2439

GCAC (614)

ptc-miR156g
TTGACAGAAGATAGAG
0.9
2440

AGCAC (615)

ptc-miR156h
TTGACAGAAGATAGAG
0.9
2441

AGCAC (616)

ptc-miR156i
TTGACAGAAGATAGAG
0.9
2442

AGCAC (617)

ptc-miR156j
TTGACAGAAGATAGAG
0.9
2443

AGCAC (618)

ptc-miR156k
TGACAGAAGAGAGGGA
0.8
2444

GCAC (619)

rco-miR156a
TGACAGAAGAGAGTGA
0.85
2445

GCACA (620)

rco-miR156b
TGACAGAAGAGAGTGA
0.85
2446

GCACA (621)

rco-miR156c
TGACAGAAGAGAGTGA
0.85
2447

GCACA (622)

rco-miR156d
TGACAGAAGAGAGTGA
0.85
2448

GCACA (623)

rco-miR156e
TGACAGAAGAGAGAGA
0.9
2449

GCACA (624)

rco-miR156f
TTGACAGAAGATAGAG
0.9
2450

AGCAC (625)

rco-miR156g
TTGACAGAAGATAGAG
0.9
2451

AGCAC (626)

rco-miR156h
TTGACAGAAGATAGAG
0.9
2452

AGCAC (627)

sbi-miR156a
TGACAGAAGAGAGTGA
0.8
2453

GCAC (628)

sbi-miR156b
TGACAGAAGAGAGTGA
0.8
2454

GCAC (629)

sbi-miR156c
TGACAGAAGAGAGTGA
0.8
2455

GCAC (630)

sbi-miR156d
TGACAGAAGAGAGAGA
0.9
2456

GCACA (631)

sbi-miR156e
TGACAGAAGAGAGCGA
0.8
2457

GCAC (632)

sbi-miR156f
TGACAGAAGAGAGTGA
0.8
2458

GCAC (633)

sbi-miR156g
TGACAGAAGAGAGTGA
0.8
2459

GCAC (634)

sbi-miR156h
TGACAGAAGAGAGTGA
0.8
2460

GCAC (635)

sbi-miR156i
TGACAGAAGAGAGTGA
0.8
2461

GCAC (636)

sly-miR156a
TTGACAGAAGATAGAG
0.9
2462

AGCAC (637)

sly-miR156b
TTGACAGAAGATAGAG
0.9
2463

AGCAC (638)

sly-miR156c
TTGACAGAAGATAGAG
0.9
2464

AGCAC (639)

smo-miR156a
CGACAGAAGAGAGTGA
0.8
2465

GCAC (640)

smo-miR156b
CTGACAGAAGATAGAG
0.9
2466

AGCAC (641)

smo-miR156c
TTGACAGAAGAAAGAG
0.85
2467

AGCAC (642)

smo-miR156d
TTGACAGAAGACAGGG
0.8
2468

AGCAC (643)

sof-miR156
TGACAGAAGAGAGTGA
0.8
2469

GCAC (644)

ssp-miR156
TGACAGAAGAGAGTGA
0.85
2470

GCACA (645)

tae-miR156
TGACAGAAGAGAGTGA
0.85
2471

GCACA (646)

tcc-miR156a
TGACAGAAGAGAGAGA
0.9
2472

GCACA (647)

tcc-miR156b
TGACAGAAGAGAGTGA
0.8
2473

GCAC (648)

tcc-miR156c
TGACAGAAGAGAGTGA
0.8
2474

GCAC (649)

tcc-miR156d
TGACAGAAGAGAGTGA
0.8
2475

GCAC (650)

tcc-miR156e
TTGACAGAAGATAGAG
0.9
2476

AGCAC (651)

tcc-miR156f
TTGACAGAAGATAGAG
0.9
2477

AGCAC (652)

tcc-miR156g
TGACAGAAGAGAGTGA
0.8
2478

GCAC (653)

vvi-miR156a
TGACAGAAGAGAGGGA
0.8
2479

GCAC (654)

vvi-miR156b
TGACAGAAGAGAGTGA
0.8
2480

GCAC (655)

vvi-miR156c
TGACAGAAGAGAGTGA
0.8
2481

GCAC (656)

vvi-miR156d
TGACAGAAGAGAGTGA
0.8
2482

GCAC (657)

vvi-miR156e
TGACAGAGGAGAGTGA
0.75
2483

GCAC (658)

vvi-miR156f
TTGACAGAAGATAGAG
0.9
2484

AGCAC (659)

vvi-miR156g
TTGACAGAAGATAGAG
0.9
2485

AGCAC (660)

vvi-miR156h
TGACAGAAGAGAGAGA
0.8
2486

GCAT (661)

vvi-miR156i
TTGACAGAAGATAGAG
0.9
2487

AGCAC (662)

zma-miR156a
TGACAGAAGAGAGTGA
0.8
2488

GCAC (663)

zma-miR156b
TGACAGAAGAGAGTGA
0.8
2489

GCAC (664)

zma-miR156c
TGACAGAAGAGAGTGA
0.8
2490

GCAC (665)

zma-miR156d
TGACAGAAGAGAGTGA
0.8
2491

GCAC (666)

zma-miR156e
TGACAGAAGAGAGTGA
0.8
2492

GCAC (667)

zma-miR156f
TGACAGAAGAGAGTGA
0.8
2493

GCAC (668)

zma-miR156g
TGACAGAAGAGAGTGA
0.8
2494

GCAC (669)

zma-miR156h
TGACAGAAGAGAGTGA
0.8
2495

GCAC (670)

zma-miR156i
TGACAGAAGAGAGTGA
0.8
2496

GCAC (671)

zma-miR156j
TGACAGAAGAGAGAGA
0.9
2497

GCACA (672)

zma-miR156k
TGACAGAAGAGAGCGA
0.8
2498

GCAC (673)

zma-miR1561
TGACAGAAGAGAGTGA
0.8
2499

GCAC (674)

gma-
ahy-miR157a-5p
TTGACAGAAGATAGAG
0.95
2500

miR157c

AGCAC (675)

ahy-miR157k
TTGACAGAAGAGAGAG
0.9
2501

AGCAC (676)

aly-miR157a
TTGACAGAAGATAGAG
0.95
2502

AGCAC (677)

aly-miR157b
TTGACAGAAGATAGAG
0.95
2503

AGCAC (678)

aly-miR157c
TTGACAGAAGATAGAG
0.95
2504

AGCAC (679)

aly-miR157d
TGACAGAAGATAGAGA
0.95
2505

GCAC (680)

ath-miR157a
TTGACAGAAGATAGAG
0.95
2506

AGCAC (681)

ath-miR157b
TTGACAGAAGATAGAG
0.95
2507

AGCAC (682)

ath-miR157c
TTGACAGAAGATAGAG
0.95
2508

AGCAC (683)

ath-miR157d
TGACAGAAGATAGAGA
0.95
2509

GCAC (684)

ath-miR157m
TTGACAGAAGAGAGAG
0.9
2510

AGCAC (685)

bol-miR157a
TTGACAGAAGATAGAG
0.95
2511

AGCAC (686)

bra-miR157a
TTGACAGAAGATAGAG
0.95
2512

AGCAC (687)

can-miR157
TTGACAGAAGAGAGAG
0.9
2513

AGCAC (688)

ghr-miR157
ATGACAGAAGAGAGAG
0.9
2514

AGCAC (689)

gma-miR157r
TTGACAGAAGAGAGAG
0.9
2515

AGCAC (690)

gra-miR157a
TTGACAGAAGATAGAG
0.95
2516

AGCAC (691)

gra-miR157b
TTGACAGAAGATAGAG
0.95
2517

AGCAC (692)

gra-miR157c
TTGACAGAAGAGAGAG
0.9
2518

AGCAC (693)

gra-miR157d
TTGACAGAAGAGAGAG
0.9
2519

AGCAC (694)

han-miR157a
TTGACAGAAGATAGAG
0.95
2520

AGCAC (695)

han-miR157b
TTGACAGAAGAGAGAG
0.9
2521

AGCAC (696)

iba-miR157
TTGACAGAAGATAGAG
0.9
2522

AGCAT (697)

ini-miR157a
TTGACAGAAGATAGAG
0.9
2523

AGCAT (698)

ini-miR157b
TTGACAGAAGATAGAG
0.9
2524

AGCAT (699)

lja-miR157a
TTGACAGAAGATAGAG
0.95
2525

AGCAC (700)

lja-miR157b
TTGACAGAAGAGAGAG
0.9
2526

AGCAC (701)

lja-miR157c
TTGACAGAAGATAGAG
0.9
2527

AGCAT (702)

lsa-miR157
TTGACAGAAGAGAGAG
0.9
2528

AGCAC (703)

mtr-miR157
TTGACAGAAGATAGAG
0.9
2529

GGCAC (704)

nad-miR157a
TTGACAGAAGACAGAG
0.95
2530

AGCAC (705)

nad-miR157b
TTGACAGAAGACAGAG
0.95
2531

AGCAC (706)

nad-miR157c
TTGACAGAAGACAGAG
0.95
2532

AGCAC (707)

nbe-miR157a
TTGACAGAAGAGAGAG
0.9
2533

AGCAC (708)

nbe-miR157b
TTGACAGAAGAGAGAG
0.9
2534

AGCAC (709)

nta-miR157
TTGACAGAAGATAGAG
0.95
2535

AGCAC (710)

pam-miR157
TTGACAGAAGAGAGAG
0.9
2536

AGCAC (711)

par-miR157
TTGACAGAAGAGAGAG
0.9
2537

AGCAC (712)

pco-miR157
TTGACAGAAGATAGAG
0.9
2538

AGCAT (713)

pts-miR157
TTGACAGAAGAGAGAG
0.9
2539

AGCAC (714)

sbi-miR157
TTGACAGAAGAGAGTG
0.86
2540

AGCAC (715)

sin-miR157
TTGACAGAAGAGAGAG
0.9
2541

AGCAC (716)

sly-miR157a
TTGACAGAAGATAGAG
0.9
2542

AGCAT (717)

sly-miR157b
TTGACAGAAGATAGAG
0.9
2543

AGCAT (718)

sly-miR157c
TTGACAGAAGATAGAG
0.9
2544

AGCAT (719)

stu-miR157a
TTGACAGAAGATAGAG
0.95
2545

AGCAC (720)

stu-miR157b
TTGACAGAAGAGAGAG
0.9
2546

AGCAC (721)

stu-miR157c
TTGACAGAAGAGAGAG
0.9
2547

AGCAC (722)

zel-miR157
TTGACAGAAGAGAGAG
0.9
2548

AGCAC (723)

zma-miR157m
TTGACAGAAGAGAGAG
0.9
2549

AGCAC (724)

iba-miR157
ahy-miR157a-5p
TTGACAGAAGATAGAG
0.95
2550

AGCAC (725)

ahy-miR157k
TTGACAGAAGAGAGAG
0.9
2551

AGCAC (726)

aly-miR157a
TTGACAGAAGATAGAG
0.95
2552

AGCAC (727)

aly-miR157b
TTGACAGAAGATAGAG
0.95
2553

AGCAC (728)

aly-miR157c
TTGACAGAAGATAGAG
0.95
2554

AGCAC (729)

aly-miR157d
TGACAGAAGATAGAGA
0.9
2555

GCAC (730)

ath-miR157a
TTGACAGAAGATAGAG
0.95
2556

AGCAC (731)

ath-miR157b
TTGACAGAAGATAGAG
0.95
2557

AGCAC (732)

ath-miR157c
TTGACAGAAGATAGAG
0.95
2558

AGCAC (733)

ath-miR157d
TGACAGAAGATAGAGA
0.9
2559

GCAC (734)

ath-miR157m
TTGACAGAAGAGAGAG
0.9
2560

AGCAC (735)

bol-miR157a
TTGACAGAAGATAGAG
0.95
2561

AGCAC (736)

bra-miR157a
TTGACAGAAGATAGAG
0.95
2562

AGCAC (737)

can-miR157
TTGACAGAAGAGAGAG
0.9
2563

AGCAC (738)

ghr-miR157
ATGACAGAAGAGAGAG
0.86
2564

AGCAC (739)

gma-miR157c
TGACAGAAGACTAGAG
0.9
2565

AGCAC (740)

gma-miR157r
TTGACAGAAGAGAGAG
0.9
2566

AGCAC (741)

gra-miR157a
TTGACAGAAGATAGAG
0.95
2567

AGCAC (742)

gra-miR157b
TTGACAGAAGATAGAG
0.95
2568

AGCAC (743)

gra-miR157c
TTGACAGAAGAGAGAG
0.9
2569

AGCAC (744)

gra-miR157d
TTGACAGAAGAGAGAG
0.9
2570

AGCAC (745)

han-miR157a
TTGACAGAAGATAGAG
0.95
2571

AGCAC (746)

han-miR157b
TTGACAGAAGAGAGAG
0.9
2572

AGCAC (747)

ini-miR157a
TTGACAGAAGATAGAG
1
2573

AGCAT (748)

ini-miR157b
TTGACAGAAGATAGAG
1
2574

AGCAT (749)

lja-miR157a
TTGACAGAAGATAGAG
0.95
2575

AGCAC (750)

lja-miR157b
TTGACAGAAGAGAGAG
0.9
2576

AGCAC (751)

lja-miR157c
TTGACAGAAGATAGAG
1
2577

AGCAT (752)

lsa-miR157
TTGACAGAAGAGAGAG
0.9
2578

AGCAC (753)

mtr-miR157
TTGACAGAAGATAGAG
0.9
2579

GGCAC (754)

nad-miR157a
TTGACAGAAGACAGAG
0.9
2580

AGCAC (755)

nad-miR157b
TTGACAGAAGACAGAG
0.9
2581

AGCAC (756)

nad-miR157c
TTGACAGAAGACAGAG
0.9
2582

AGCAC (757)

nbe-miR157a
TTGACAGAAGAGAGAG
0.9
2583

AGCAC (758)

nbe-miR157b
TTGACAGAAGAGAGAG
0.9
2584

AGCAC (759)

nta-miR157
TTGACAGAAGATAGAG
0.95
2585

AGCAC (760)

pam-miR157
TTGACAGAAGAGAGAG
0.9
2586

AGCAC (761)

par-miR157
TTGACAGAAGAGAGAG
0.9
2587

AGCAC (762)

pco-miR157
TTGACAGAAGATAGAG
1
2588

AGCAT (763)

pts-miR157
TTGACAGAAGAGAGAG
0.9
2589

AGCAC (764)

sbi-miR157
TTGACAGAAGAGAGTG
0.86
2590

AGCAC (765)

sin-miR157
TTGACAGAAGAGAGAG
0.9
2591

AGCAC (766)

sly-miR157a
TTGACAGAAGATAGAG
1
2592

AGCAT (767)

sly-miR157b
TTGACAGAAGATAGAG
1
2593

AGCAT (768)

sly-miR157c
TTGACAGAAGATAGAG
1
2594

AGCAT (769)

stu-miR157a
TTGACAGAAGATAGAG
0.95
2595

AGCAC (770)

stu-miR157b
TTGACAGAAGAGAGAG
0.9
2596

AGCAC (771)

stu-miR157c
TTGACAGAAGAGAGAG
0.9
2597

AGCAC (772)

zel-miR157
TTGACAGAAGAGAGAG
0.9
2598

AGCAC (773)

zma-miR157m
TTGACAGAAGAGAGAG
0.9
2599

AGCAC (774)

mdm-
gma-miR482b-5p
TATGGGGGGATTGGGA
0.62
2600

miR482a-

AGGAAT (775)

5p

mdo-miR482*
GGAATGGGCTGTTTGGG
1
2601

AACA (776)

pvu-miR482*
GGAATGGGCTGATTGG
0.95
2602

GAAGCA (777)

osa-
acb-miR159
TTGGACTGAAGGGAGCT
0.86
2603

miR159e

CCCT (778)

aha-miR159
TTGGACTGAAGGGAGCT
0.86
2604

CCCT (779)

ahi-miR159
TTGGACTGAAGGGAGCT
0.86
2605

CCCT (780)

ahy-miR159
TTTGGATTGAAGGGAGC
0.86
2606

TCTA (781)

aly-miR159a
TTTGGATTGAAGGGAGC
0.86
2607

TCTA (782)

aly-miR159b
TTTGGATTGAAGGGAGC
0.9
2608

TCTT (783)

aly-miR159c
TTTGGATTGAAGGGAGC
0.95
2609

TCCT (784)

ape-miR159
TTGGACTGAAGGGAGCT
0.86
2610

CCCT (785)

aqc-miR159
TTTGGACTGAAGGGAGC
0.81
2611

TCTA (786)

ath-miR159a
TTTGGATTGAAGGGAGC
0.86
2612

TCTA (787)

ath-miR159b
TTTGGATTGAAGGGAGC
0.9
2613

TCTT (788)

ath-miR159c
TTTGGATTGAAGGGAGC
0.95
2614

TCCT (789)

bdi-miR159
CTTGGATTGAAGGGAGC
0.86
2615

TCT (790)

bna-miR159
TTTGGATTGAAGGGAGC
0.86
2616

TCTA (791)

bra-miR159a
TTTGGATTGAAGGGAGC
0.86
2617

TCTA (792)

bvl-miR159
TTGGACTGAAGGGAGCT
0.86
2618

CCCT (793)

cmi-miR159
TTGGACTGAAGGGAGCT
0.86
2619

CCCT (794)

cor-miR159
TTGGACTGAAGGGAGCT
0.86
2620

CCCT (795)

crb-miR159
TTGGACTGAAGGGAGCT
0.86
2621

CCCT (796)

csi-miR159
TTTGGATTGAAGGGAGC
0.86
2622

TCTA (797)

dso-miR159
TTGGACTGAAGGGAGCT
0.86
2623

CCCT (798)

ech-miR159
TTGGACTGAAGGGAGCT
0.86
2624

CCCT (799)

fal-miR159
TTGGACTGAAGGGAGCT
0.86
2625

CCCT (800)

far-miR159
TTTGGATTGAAGGGAGC
0.86
2626

TCTG (801)

gma-miR159a-3p
TTTGGATTGAAGGGAGC
0.86
2627

TCTA (802)

gma-miR159b
ATTGGAGTGAAGGGAG
0.9
2628

CTCCA (803)

gma-miR159c
ATTGGAGTGAAGGGAG
0.9
2629

CTCCG (804)

hvu-miR159a
TTTGGATTGAAGGGAGC
0.86
2630

TCTG (805)

hvu-miR159b
TTTGGATTGAAGGGAGC
0.86
2631

TCTG (806)

hvv-miR159a
TTTGGATTGAAGGGAGC
0.86
2632

TCTG (807)

hvv-miR159b
TTTGGATTGAAGGGAGC
0.86
2633

TCTG (808)

ltu-miR159
TTTGGATTGAAGGGAGC
0.86
2634

TCTA (809)

mma-miR159
TTGGACTGAAGGGAGCT
0.86
2635

CCCT (810)

mtr-miR159a
TTTGGATTGAAGGGAGC
0.86
2636

TCTA (811)

mtr-miR159b
ATTGAATTGAAGGGAG
0.86
2637

CAACT (812)

mtr-miR159c
TTTGGATTGAAGGGAGC
0.86
2638

TCTA (813)

nof-miR159
TTGGACTGAAGGGAGCT
0.86
2639

CCCT (814)

oru-miR159
TTTGGATTGAAGGGAGC
0.86
2640

TCTG (815)

osa-miR159a
TTTGGATTGAAGGGAGC
0.86
2641

TCTG (816)

osa-miR159a.1
TTTGGATTGAAGGGAGC
0.86
2642

TCTG (817)

osa-miR159b
TTTGGATTGAAGGGAGC
0.86
2643

TCTG (818)

osa-miR159c
ATTGGATTGAAGGGAG
0.95
2644

CTCCA (819)

osa-miR159d
ATTGGATTGAAGGGAG
0.95
2645

CTCCG (820)

osa-miR159f
CTTGGATTGAAGGGAGC
0.86
2646

TCTA (821)

osa-miR159m
TTTGGATTGAAGGGAGC
0.86
2647

TCTG (822)

pgl-miR159
TTTGGATTGAAGGGAGC
0.86
2648

TCTG (823)

psi-miR159
CTTGGATTGAAGGGAGC
0.9
2649

TCCA (824)

pta-miR159a
TTGGATTGAAGGGAGCT
0.9
2650

CCA (825)

pta-miR159b
TTGGATTGAAGAGAGCT
0.86
2651

CCC (826)

pta-miR159c
CTTGGATTGAAGGGAGC
0.9
2652

TCCC (827)

ptc-miR159a
TTTGGATTGAAGGGAGC
0.86
2653

TCTA (828)

ptc-miR159b
TTTGGATTGAAGGGAGC
0.86
2654

TCTA (829)

ptc-miR159c
TTTGGATTGAAGGGAGC
0.86
2655

TCTA (830)

ptc-miR159d
CTTGGATTGAAGGGAGC
0.95
2656

TCCT (831)

ptc-miR159e
CTTGGGGTGAAGGGAG
0.86
2657

CTCCT (832)

ptc-miR159f
ATTGGAGTGAAGGGAG
0.86
2658

CTCGA (833)

pvu-miR159
TTTGGATTGAAGGGAGC
0.86
2659

TCTA (834)

pvu-miR159.2
CTTCCATATCTGGGGAG
0.62
2660

CTTC (835)

pvu-miR159a.1
TTTGGATTGAAGGGAGC
0.86
2661

TCTA (836)

pvu-miR159a.2
CTTCCATATCTGGGGAG
0.62
2662

CTTC (837)

rco-miR159
TTTGGATTGAAGGGAGC
0.86
2663

TCTA (838)

rin-miR159
TTGGACTGAAGGGAGCT
0.86
2664

CCCT (839)

sar-miR159
TTTGGATTGAAGGGAGC
0.86
2665

TCTG (840)

sbi-miR159a
TTTGGATTGAAGGGAGC
0.86
2666

TCTG (841)

sbi-miR159b
CTTGGATTGAAGGGAGC
0.95
2667

TCCT (842)

sly-miR159
TTTGGATTGAAGGGAGC
0.86
2668

TCTA (843)

smo-miR159
CTTGGATTGAAGGGAGC
0.9
2669

TCCC (844)

sof-miR159a
TTTGGATTGAAGGGAGC
0.86
2670

TCTG (845)

sof-miR159b
TTTGGATTGAAGGGAGC
0.86
2671

TCTG (846)

sof-miR159c
CTTGGATTGAAGGGAGC
0.95
2672

TCCT (847)

sof-miR159d
TTTGGATTGAAGGGAGC
0.86
2673

TCTG (848)

sof-miR159e
TTTGGATTGAAAGGAGC
0.86
2674

TCTT (849)

spr-miR159
TTTGGATTGAAGGGAGC
0.86
2675

TCTG (850)

ssp-miR159a
TTTGGATTGAAGGGAGC
0.86
2676

TCTG (851)

svi-miR159
TTGGACTGAAGGGAGCT
0.86
2677

CCCT (852)

tae-miR159a
TTTGGATTGAAGGGAGC
0.86
2678

TCTG (853)

tae-miR159b
TTTGGATTGAAGGGAGC
0.86
2679

TCTG (854)

tar-miR159
TTGGACTGAAGGGAGCT
0.86
2680

CCCT (855)

vvi-miR159a
CTTGGAGTGAAGGGAG
0.81
2681

CTCTC (856)

vvi-miR159b
CTTGGAGTGAAGGGAG
0.81
2682

CTCTC (857)

vvi-miR159c
TTTGGATTGAAGGGAGC
0.86
2683

TCTA (858)

zma-miR159a
TTTGGATTGAAGGGAGC
0.86
2684

TCTG (859)

zma-miR159b
TTTGGATTGAAGGGAGC
0.86
2685

TCTG (860)

zma-miR159c
CTTGGATTGAAGGGAGC
0.95
2686

TCCT (861)

zma-miR159d
CTTGGATTGAAGGGAGC
0.95
2687

TCCT (862)

zma-miR159e
ATTGGTTTGAAGGGAGC
0.9
2688

TCCA (863)

zma-miR159f
TTTGGATTGAAGGGAGC
0.86
2689

TCTG (864)

zma-miR159g
TTTGGAGTGAAGGGAGT
0.76
2690

TCTG (865)

zma-miR159h
TTTGGAGTGAAGGGAG
0.81
2691

CTCTG (866)

zma-miR159i
TTTGGAGTGAAGGGAG
0.81
2692

CTCTG (867)

zma-miR159j
TTTGGATTGAAGGGAGC
0.86
2693

TCTG (868)

zma-miR159k
TTTGGATTGAAGGGAGC
0.86
2694

TCTG (869)

zma-miR159m
TTTGGATTGAAGGGAGC
0.86
2695

TCTG (870)

osa-
acb-miR159
TTGGACTGAAGGGAGCT
0.81
2696

miR159f

CCCT (871)

aha-miR159
TTGGACTGAAGGGAGCT
0.81
2697

CCCT (872)

ahi-miR159
TTGGACTGAAGGGAGCT
0.81
2698

CCCT (873)

ahy-miR159
TTTGGATTGAAGGGAGC
0.95
2699

TCTA (874)

aly-miR159a
TTTGGATTGAAGGGAGC
0.95
2700

TCTA (875)

aly-miR159b
TTTGGATTGAAGGGAGC
0.9
2701

TCTT (876)

aly-miR159c
TTTGGATTGAAGGGAGC
0.86
2702

TCCT (877)

ape-miR159
TTGGACTGAAGGGAGCT
0.81
2703

CCCT (878)

aqc-miR159
TTTGGACTGAAGGGAGC
0.9
2704

TCTA (879)

ath-miR159a
TTTGGATTGAAGGGAGC
0.95
2705

TCTA (880)

ath-miR159b
TTTGGATTGAAGGGAGC
0.9
2706

TCTT (881)

ath-miR159c
TTTGGATTGAAGGGAGC
0.86
2707

TCCT (882)

bdi-miR159
CTTGGATTGAAGGGAGC
0.95
2708

TCT (883)

bna-miR159
TTTGGATTGAAGGGAGC
0.95
2709

TCTA (884)

bra-miR159a
TTTGGATTGAAGGGAGC
0.95
2710

TCTA (885)

bvl-miR159
TTGGACTGAAGGGAGCT
0.81
2711

CCCT (886)

cmi-miR159
TTGGACTGAAGGGAGCT
0.81
2712

CCCT (887)

cor-miR159
TTGGACTGAAGGGAGCT
0.81
2713

CCCT (888)

crb-miR159
TTGGACTGAAGGGAGCT
0.81
2714

CCCT (889)

csi-miR159
TTTGGATTGAAGGGAGC
0.95
2715

TCTA (890)

dso-miR159
TTGGACTGAAGGGAGCT
0.81
2716

CCCT (891)

ech-miR159
TTGGACTGAAGGGAGCT
0.81
2717

CCCT (892)

fal-miR159
TTGGACTGAAGGGAGCT
0.81
2718

CCCT (893)

far-miR159
TTTGGATTGAAGGGAGC
0.9
2719

TCTG (894)

gma-miR159a-3p
TTTGGATTGAAGGGAGC
0.95
2720

TCTA (895)

gma-miR159b
ATTGGAGTGAAGGGAG
0.86
2721

CTCCA (896)

gma-miR159c
ATTGGAGTGAAGGGAG
0.81
2722

CTCCG (897)

hvu-miR159a
TTTGGATTGAAGGGAGC
0.9
2723

TCTG (898)

hvu-miR159b
TTTGGATTGAAGGGAGC
0.9
2724

TCTG (899)

hvv-miR159a
TTTGGATTGAAGGGAGC
0.9
2725

TCTG (900)

hvv-miR159b
TTTGGATTGAAGGGAGC
0.9
2726

TCTG (901)

ltu-miR159
TTTGGATTGAAGGGAGC
0.95
2727

TCTA (902)

mma-miR159
TTGGACTGAAGGGAGCT
0.81
2728

CCCT (903)

mtr-miR159a
TTTGGATTGAAGGGAGC
0.95
2729

TCTA (904)

mtr-miR159b
ATTGAATTGAAGGGAG
0.71
2730

CAACT (905)

mtr-miR159c
TTTGGATTGAAGGGAGC
0.95
2731

TCTA (906)

nof-miR159
TTGGACTGAAGGGAGCT
0.81
2732

CCCT (907)

oru-miR159
TTTGGATTGAAGGGAGC
0.9
2733

TCTG (908)

osa-miR159a
TTTGGATTGAAGGGAGC
0.9
2734

TCTG (909)

osa-miR159a.1
TTTGGATTGAAGGGAGC
0.9
2735

TCTG (910)

osa-miR159b
TTTGGATTGAAGGGAGC
0.9
2736

TCTG (911)

osa-miR159c
ATTGGATTGAAGGGAG
0.9
2737

CTCCA (912)

osa-miR159d
ATTGGATTGAAGGGAG
0.86
2738

CTCCG (913)

osa-miR159e
ATTGGATTGAAGGGAG
0.86
2739

CTCCT (914)

osa-miR159m
TTTGGATTGAAGGGAGC
0.9
2740

TCTG (915)

pgl-miR159
TTTGGATTGAAGGGAGC
0.9
2741

TCTG (916)

psi-miR159
CTTGGATTGAAGGGAGC
0.95
2742

TCCA (917)

pta-miR159a
TTGGATTGAAGGGAGCT
0.9
2743

CCA (918)

pta-miR159b
TTGGATTGAAGAGAGCT
0.81
2744

CCC (919)

pta-miR159c
CTTGGATTGAAGGGAGC
0.9
2745

TCCC (920)

ptc-miR159a
TTTGGATTGAAGGGAGC
0.95
2746

TCTA (921)

ptc-miR159b
TTTGGATTGAAGGGAGC
0.95
2747

TCTA (922)

ptc-miR159c
TTTGGATTGAAGGGAGC
0.95
2748

TCTA (923)

ptc-miR159d
CTTGGATTGAAGGGAGC
0.9
2749

TCCT (924)

ptc-miR159e
CTTGGGGTGAAGGGAG
0.81
2750

CTCCT (925)

ptc-miR159f
ATTGGAGTGAAGGGAG
0.86
2751

CTCGA (926)

pvu-miR159
TTTGGATTGAAGGGAGC
0.95
2752

TCTA (927)

pvu-miR159.2
CTTCCATATCTGGGGAG
0.62
2753

CTTC (928)

pvu-miR159a.1
TTTGGATTGAAGGGAGC
0.95
2754

TCTA (929)

pvu-miR159a.2
CTTCCATATCTGGGGAG
0.62
2755

CTTC (930)

rco-miR159
TTTGGATTGAAGGGAGC
0.95
2756

TCTA (931)

rin-miR159
TTGGACTGAAGGGAGCT
0.81
2757

CCCT (932)

sar-miR159
TTTGGATTGAAGGGAGC
0.9
2758

TCTG (933)

sbi-miR159a
TTTGGATTGAAGGGAGC
0.9
2759

TCTG (934)

sbi-miR159b
CTTGGATTGAAGGGAGC
0.9
2760

TCCT (935)

sly-miR159
TTTGGATTGAAGGGAGC
0.95
2761

TCTA (936)

smo-miR159
CTTGGATTGAAGGGAGC
0.9
2762

TCCC (937)

sof-miR159a
TTTGGATTGAAGGGAGC
0.9
2763

TCTG (938)

sof-miR159b
TTTGGATTGAAGGGAGC
0.9
2764

TCTG (939)

sof-miR159c
CTTGGATTGAAGGGAGC
0.9
2765

TCCT (940)

sof-miR159d
TTTGGATTGAAGGGAGC
0.9
2766

TCTG (941)

sof-miR159e
TTTGGATTGAAAGGAGC
0.86
2767

TCTT (942)

spr-miR159
TTTGGATTGAAGGGAGC
0.9
2768

TCTG (943)

ssp-miR159a
TTTGGATTGAAGGGAGC
0.9
2769

TCTG (944)

svi-miR159
TTGGACTGAAGGGAGCT
0.81
2770

CCCT (945)

tae-miR159a
TTTGGATTGAAGGGAGC
0.9
2771

TCTG (946)

tae-miR159b
TTTGGATTGAAGGGAGC
0.9
2772

TCTG (947)

tar-miR159
TTGGACTGAAGGGAGCT
0.81
2773

CCCT (948)

vvi-miR159a
CTTGGAGTGAAGGGAG
0.9
2774

CTCTC (949)

vvi-miR159b
CTTGGAGTGAAGGGAG
0.9
2775

CTCTC (950)

vvi-miR159c
TTTGGATTGAAGGGAGC
0.95
2776

TCTA (951)

zma-miR159a
TTTGGATTGAAGGGAGC
0.9
2777

TCTG (952)

zma-miR159b
TTTGGATTGAAGGGAGC
0.9
2778

TCTG (953)

zma-miR159c
CTTGGATTGAAGGGAGC
0.9
2779

TCCT (954)

zma-miR159d
CTTGGATTGAAGGGAGC
0.9
2780

TCCT (955)

zma-miR159e
ATTGGTTTGAAGGGAGC
0.86
2781

TCCA (956)

zma-miR159f
TTTGGATTGAAGGGAGC
0.9
2782

TCTG (957)

zma-miR159g
TTTGGAGTGAAGGGAGT
0.81
2783

TCTG (958)

zma-miR159h
TTTGGAGTGAAGGGAG
0.86
2784

CTCTG (959)

zma-miR159i
TTTGGAGTGAAGGGAG
0.86
2785

CTCTG (960)

zma-miR159j
TTTGGATTGAAGGGAGC
0.9
2786

TCTG (961)

zma-miR159k
TTTGGATTGAAGGGAGC
0.9
2787

TCTG (962)

zma-miR159m
TTTGGATTGAAGGGAGC
0.9
2788

TCTG (963)

osa-
osa-miR1858b
GAGAGGAGGACGGAGT
1
2789

miR1858a

GGGGC (964)

psi-miR159
acb-miR159
TTGGACTGAAGGGAGCT
0.86
2790

CCCT (965)

aha-miR159
TTGGACTGAAGGGAGCT
0.86
2791

CCCT (966)

ahi-miR159
TTGGACTGAAGGGAGCT
0.86
2792

CCCT (967)

ahy-miR159
TTTGGATTGAAGGGAGC
0.9
2793

TCTA (968)

aly-miR159a
TTTGGATTGAAGGGAGC
0.9
2794

TCTA (969)

aly-miR159b
TTTGGATTGAAGGGAGC
0.86
2795

TCTT (970)

aly-miR159c
TTTGGATTGAAGGGAGC
0.9
2796

TCCT (971)

ape-miR159
TTGGACTGAAGGGAGCT
0.86
2797

CCCT (972)

aqc-miR159
TTTGGACTGAAGGGAGC
0.86
2798

TCTA (973)

ath-miR159a
TTTGGATTGAAGGGAGC
0.9
2799

TCTA (974)

ath-miR159b
TTTGGATTGAAGGGAGC
0.86
2800

TCTT (975)

ath-miR159c
TTTGGATTGAAGGGAGC
0.9
2801

TCCT (976)

bdi-miR159
CTTGGATTGAAGGGAGC
0.9
2802

TCT (977)

bna-miR159
TTTGGATTGAAGGGAGC
0.9
2803

TCTA (978)

bra-miR159a
TTTGGATTGAAGGGAGC
0.9
2804

TCTA (979)

bvl-miR159
TTGGACTGAAGGGAGCT
0.86
2805

CCCT (980)

cmi-miR159
TTGGACTGAAGGGAGCT
0.86
2806

CCCT (981)

cor-miR159
TTGGACTGAAGGGAGCT
0.86
2807

CCCT (982)

crb-miR159
TTGGACTGAAGGGAGCT
0.86
2808

CCCT (983)

csi-miR159
TTTGGATTGAAGGGAGC
0.9
2809

TCTA (984)

dso-miR159
TTGGACTGAAGGGAGCT
0.86
2810

CCCT (985)

ech-miR159
TTGGACTGAAGGGAGCT
0.86
2811

CCCT (986)

fal-miR159
TTGGACTGAAGGGAGCT
0.86
2812

CCCT (987)

far-miR159
TTTGGATTGAAGGGAGC
0.86
2813

TCTG (988)

gma-miR159a-3p
TTTGGATTGAAGGGAGC
0.9
2814

TCTA (989)

gma-miR159b
ATTGGAGTGAAGGGAG
0.9
2815

CTCCA (990)

gma-miR159c
ATTGGAGTGAAGGGAG
0.86
2816

CTCCG (991)

hvu-miR159a
TTTGGATTGAAGGGAGC
0.86
2817

TCTG (992)

hvu-miR159b
TTTGGATTGAAGGGAGC
0.86
2818

TCTG (993)

hvv-miR159a
TTTGGATTGAAGGGAGC
0.86
2819

TCTG (994)

hvv-miR159b
TTTGGATTGAAGGGAGC
0.86
2820

TCTG (995)

ltu-miR159
TTTGGATTGAAGGGAGC
0.9
2821

TCTA (996)

mma-miR159
TTGGACTGAAGGGAGCT
0.86
2822

CCCT (997)

mtr-miR159a
TTTGGATTGAAGGGAGC
0.9
2823

TCTA (998)

mtr-miR159b
ATTGAATTGAAGGGAG
0.76
2824

CAACT (999)

mtr-miR159c
TTTGGATTGAAGGGAGC
0.9
2825

TCTA (1000)

nof-miR159
TTGGACTGAAGGGAGCT
0.86
2826

CCCT (1001)

oru-miR159
TTTGGATTGAAGGGAGC
0.86
2827

TCTG (1002)

osa-miR159a
TTTGGATTGAAGGGAGC
0.86
2828

TCTG (1003)

osa-miR159a.1
TTTGGATTGAAGGGAGC
0.86
2829

TCTG (1004)

osa-miR159a.2
TTGCATGCCCCAGGAGC
0.62
2830

TGCA (1005)

osa-miR159b
TTTGGATTGAAGGGAGC
0.86
2831

TCTG (1006)

osa-miR159c
ATTGGATTGAAGGGAG
0.95
2832

CTCCA (1007)

osa-miR159d
ATTGGATTGAAGGGAG
0.9
2833

CTCCG (1008)

osa-miR159e
ATTGGATTGAAGGGAG
0.9
2834

CTCCT (1009)

osa-miR159f
CTTGGATTGAAGGGAGC
0.95
2835

TCTA (1010)

osa-miR159m
TTTGGATTGAAGGGAGC
0.86
2836

TCTG (1011)

pgl-miR159
TTTGGATTGAAGGGAGC
0.86
2837

TCTG (1012)

pta-miR159a
TTGGATTGAAGGGAGCT
0.95
2838

CCA (1013)

pta-miR159b
TTGGATTGAAGAGAGCT
0.86
2839

CCC (1014)

pta-miR159c
CTTGGATTGAAGGGAGC
0.95
2840

TCCC (1015)

ptc-miR159a
TTTGGATTGAAGGGAGC
0.9
2841

TCTA (1016)

ptc-miR159b
TTTGGATTGAAGGGAGC
0.9
2842

TCTA (1017)

ptc-miR159c
TTTGGATTGAAGGGAGC
0.9
2843

TCTA (1018)

ptc-miR159d
CTTGGATTGAAGGGAGC
0.95
2844

TCCT (1019)

ptc-miR159e
CTTGGGGTGAAGGGAG
0.86
2845

CTCCT (1020)

ptc-miR159f
ATTGGAGTGAAGGGAG
0.86
2846

CTCGA (1021)

pvu-miR159
TTTGGATTGAAGGGAGC
0.9
2847

TCTA (1022)

pvu-miR159.2
CTTCCATATCTGGGGAG
0.67
2848

CTTC (1023)

pvu-miR159a.1
TTTGGATTGAAGGGAGC
0.9
2849

TCTA (1024)

pvu-miR159a.2
CTTCCATATCTGGGGAG
0.67
2850

CTTC (1025)

rco-miR159
TTTGGATTGAAGGGAGC
0.9
2851

TCTA (1026)

rin-miR159
TTGGACTGAAGGGAGCT
0.86
2852

CCCT (1027)

sar-miR159
TTTGGATTGAAGGGAGC
0.86
2853

TCTG (1028)

sbi-miR159a
TTTGGATTGAAGGGAGC
0.86
2854

TCTG (1029)

sbi-miR159b
CTTGGATTGAAGGGAGC
0.95
2855

TCCT (1030)

sly-miR159
TTTGGATTGAAGGGAGC
0.9
2856

TCTA (1031)

smo-miR159
CTTGGATTGAAGGGAGC
0.95
2857

TCCC (1032)

sof-miR159a
TTTGGATTGAAGGGAGC
0.86
2858

TCTG (1033)

sof-miR159b
TTTGGATTGAAGGGAGC
0.86
2859

TCTG (1034)

sof-miR159c
CTTGGATTGAAGGGAGC
0.95
2860

TCCT (1035)

sof-miR159d
TTTGGATTGAAGGGAGC
0.86
2861

TCTG (1036)

sof-miR159e
TTTGGATTGAAAGGAGC
0.81
2862

TCTT (1037)

spr-miR159
TTTGGATTGAAGGGAGC
0.86
2863

TCTG (1038)

ssp-miR159a
TTTGGATTGAAGGGAGC
0.86
2864

TCTG (1039)

svi-miR159
TTGGACTGAAGGGAGCT
0.86
2865

CCCT (1040)

tae-miR159a
TTTGGATTGAAGGGAGC
0.86
2866

TCTG (1041)

tae-miR159b
TTTGGATTGAAGGGAGC
0.86
2867

TCTG (1042)

tar-miR159
TTGGACTGAAGGGAGCT
0.86
2868

CCCT (1043)

vvi-miR159a
CTTGGAGTGAAGGGAG
0.86
2869

CTCTC (1044)

vvi-miR159b
CTTGGAGTGAAGGGAG
0.86
2870

CTCTC (1045)

vvi-miR159c
TTTGGATTGAAGGGAGC
0.9
2871

TCTA (1046)

zma-miR159a
TTTGGATTGAAGGGAGC
0.86
2872

TCTG (1047)

zma-miR159b
TTTGGATTGAAGGGAGC
0.86
2873

TCTG (1048)

zma-miR159c
CTTGGATTGAAGGGAGC
0.95
2874

TCCT (1049)

zma-miR159d
CTTGGATTGAAGGGAGC
0.95
2875

TCCT (1050)

zma-miR159e
ATTGGTTTGAAGGGAGC
0.9
2876

TCCA (1051)

zma-miR159f
TTTGGATTGAAGGGAGC
0.86
2877

TCTG (1052)

zma-miR159g
TTTGGAGTGAAGGGAGT
0.76
2878

TCTG (1053)

zma-miR159h
TTTGGAGTGAAGGGAG
0.81
2879

CTCTG (1054)

zma-miR159i
TTTGGAGTGAAGGGAG
0.81
2880

CTCTG (1055)

zma-miR159j
TTTGGATTGAAGGGAGC
0.86
2881

TCTG (1056)

zma-miR159k
TTTGGATTGAAGGGAGC
0.86
2882

TCTG (1057)

zma-miR159m
TTTGGATTGAAGGGAGC
0.86
2883

TCTG (1058)

pta-
ahy-miR156a
TGACAGAAGAGAGAGA
0.8
2884

miR156a

GCAC (1059)

ahy-miR156b-5p
TTGACAGAAGATAGAG
0.85
2885

AGCAC (1060)

ahy-miR156c
TTGACAGAAGAGAGAG
0.8
2886

AGCAC (1061)

aly-miR156a
TGACAGAAGAGAGTGA
0.75
2887

GCAC (1062)

aly-miR156b
TGACAGAAGAGAGTGA
0.75
2888

GCAC (1063)

aly-miR156c
TGACAGAAGAGAGTGA
0.75
2889

GCAC (1064)

aly-miR156d
TGACAGAAGAGAGTGA
0.75
2890

GCAC (1065)

aly-miR156e
TGACAGAAGAGAGTGA
0.75
2891

GCAC (1066)

aly-miR156f
TGACAGAAGAGAGTGA
0.75
2892

GCAC (1067)

aly-miR156g
CGACAGAAGAGAGTGA
0.75
2893

GCAC (1068)

aly-miR156h
TGACAGAAGAAAGAGA
0.8
2894

GCAC (1069)

aqc-miR156a
TGACAGAAGATAGAGA
0.85
2895

GCAC (1070)

aqc-miR156b
TGACAGAAGATAGAGA
0.85
2896

GCAC (1071)

ath-miR156a
TGACAGAAGAGAGTGA
0.75
2897

GCAC (1072)

ath-miR156b
TGACAGAAGAGAGTGA
0.75
2898

GCAC (1073)

ath-miR156c
TGACAGAAGAGAGTGA
0.75
2899

GCAC (1074)

ath-miR156d
TGACAGAAGAGAGTGA
0.75
2900

GCAC (1075)

ath-miR156e
TGACAGAAGAGAGTGA
0.75
2901

GCAC (1076)

ath-miR156f
TGACAGAAGAGAGTGA
0.75
2902

GCAC (1077)

ath-miR156g
CGACAGAAGAGAGTGA
0.75
2903

GCAC (1078)

ath-miR156h
TGACAGAAGAAAGAGA
0.8
2904

GCAC (1079)

ath-miR156m
TGACAGAAGAGAGAGA
0.8
2905

GCAC (1080)

ath-miR156o
TGACAGAAGAGAGAGA
0.8
2906

GCAC (1081)

ath-miR156p
TGACAGAAGAGAGAGA
0.8
2907

GCAC (1082)

ath-miR156q
TGACAGAAGAGAGAGA
0.8
2908

GCAC (1083)

ath-miR156r
TGACAGAAGAGAGAGA
0.8
2909

GCAC (1084)

ath-miR156s
TGACAGAAGAGAGAGA
0.8
2910

GCAC (1085)

bdi-miR156
TGACAGAAGAGAGAGA
0.85
2911

GCACA (1086)

bdi-miR156b
TGACAGAAGAGAGTGA
0.75
2912

GCAC (1087)

bdi-miR156c
TGACAGAAGAGAGTGA
0.75
2913

GCAC (1088)

bdi-miR156d
TGACAGAAGAGAGTGA
0.75
2914

GCAC (1089)

bna-miR156a
TGACAGAAGAGAGTGA
0.8
2915

GCACA (1090)

bna-miR156b
TTGACAGAAGATAGAG
0.85
2916

AGCAC (1091)

bna-miR156c
TTGACAGAAGATAGAG
0.85
2917

AGCAC (1092)

can-miR156a
TGACAGAAGAGAGAGA
0.8
2918

GCAC (1093)

can-miR156b
TGACAGAAGAGAGGGA
0.75
2919

GCAC (1094)

cpt-miR156a
TGACAGAAGAGAGTGA
0.75
2920

GCAC (1095)

cpt-miR156b
TGACAGAAGAGAGAGA
0.8
2921

GCAC (1096)

cru-miR156
TGACAGAAGAGAGAGA
0.8
2922

GCAC (1097)

csi-miR156
TGACAGAAGAGAGTGA
0.75
2923

GCAC (1098)

csi-miR156a
TGACAGAAGAGAGAGA
0.8
2924

GCAC (1099)

csi-miR156b
TGACAGAAGAGAGAGA
0.8
2925

GCAC (1100)

ctr-miR156
TGACAGAAGAGAGTGA
0.75
2926

GCAC (1101)

eca-miR156
TGACAGAAGAGAGAGA
0.8
2927

GCAC (1102)

far-miR156a
TGACAGAAGAGAGAGA
0.85
2928

GCACA (1103)

far-miR156b
TTGACAGAAGAGAGAG
0.8
2929

AGCAC (1104)

ghr-miR156a
TGACAGAAGAGAGTGA
0.75
2930

GCAC (1105)

ghr-miR156b
TGACAGAAGAGAGTGA
0.75
2931

GCAC (1106)

ghr-miR156c
TGTCAGAAGAGAGTGA
0.75
2932

GCAC (1107)

ghr-miR156d
TGACAGAAGAGAGTGA
0.75
2933

GCAC (1108)

gma-miR156a
TGACAGAAGAGAGTGA
0.75
2934

GCAC (1109)

gma-miR156b
TGACAGAAGAGAGAGA
0.85
2935

GCACA (1110)

gma-miR156c
TTGACAGAAGATAGAG
0.85
2936

AGCAC (1111)

gma-miR156d
TTGACAGAAGATAGAG
0.85
2937

AGCAC (1112)

gma-miR156e
TTGACAGAAGATAGAG
0.85
2938

AGCAC (1113)

gma-miR156f
TTGACAGAAGAGAGAG
0.85
2939

AGCACA (1114)

gma-miR156g
ACAGAAGATAGAGAGC
0.9
2940

ACAG (1115)

gma-miR156h
TGACAGAAGAGAGAGA
0.8
2941

GCAC (1116)

gma-miR156i
TGACAGAAGAGAGAGA
0.8
2942

GCAC (1117)

han-miR156
TGACAGAAGAGAGAGA
0.8
2943

GCAC (1118)

hvs-miR156
TGACAGAAGAGAGAGA
0.8
2944

GCAC (1119)

hvu-miR156
TGACAGAAGAGAGTGA
0.8
2945

GCACA (1120)

hvv-miR156a
TGACAGAAGAGAGTGA
0.75
2946

GCAC (1121)

hvv-miR156b
TGACAGAAGAGAGAGA
0.8
2947

GCAC (1122)

hvv-miR156c
TGACAGAAGAGAGAGA
0.8
2948

GCAC (1123)

hvv-miR156d
TGACAGAAGAGAGAGA
0.8
2949

GCAC (1124)

lja-miR156
TGACAGAAGAGAGAGA
0.8
2950

GCAC (1125)

lsa-miR156
TGACAGAAGAGAGAGA
0.8
2951

GCAC (1126)

mdo-miR156a
TGACAGAAGAGAGAGA
0.8
2952

GCAC (1127)

mdo-miR156b
TGACAGAAGAGAGAGA
0.8
2953

GCAC (1128)

mtr-miR156
TGACAGAAGAGAGAGA
0.85
2954

GCACA (1129)

mtr-miR156b
TGACAGAAGAGAGTGA
0.75
2955

GCAC (1130)

mtr-miR156c
TGACAGAAGAGAGTGA
0.75
2956

GCAC (1131)

mtr-miR156d
TGACAGAAGAGAGTGA
0.75
2957

GCAC (1132)

mtr-miR156e
TTGACAGAAGATAGAG
0.85
2958

AGCAC (1133)

mtr-miR156f
TTGACAGAAGATAGAG
0.85
2959

AGCAC (1134)

mtr-miR156g
TTGACAGAAGATAGAG
0.8
2960

GGCAC (1135)

mtr-miR156h
TTGACAGAAGATAGAG
0.85
2961

AGCAC (1136)

mtr-miR156i
TGACAGAAGAGAGTGA
0.75
2962

GCAC (1137)

nbe-miR156a
TGACAGAAGAGAGAGA
0.8
2963

GCAC (1138)

nbe-miR156b
TGACAGAAGAGAGAGA
0.8
2964

GCAC (1139)

oru-miR156
TGACAGAAGAGAGTGA
0.75
2965

GCAC (1140)

osa-miR156a
TGACAGAAGAGAGTGA
0.75
2966

GCAC (1141)

osa-miR156b
TGACAGAAGAGAGTGA
0.75
2967

GCAC (1142)

osa-miR156c
TGACAGAAGAGAGTGA
0.75
2968

GCAC (1143)

osa-miR156d
TGACAGAAGAGAGTGA
0.75
2969

GCAC (1144)

osa-miR156e
TGACAGAAGAGAGTGA
0.75
2970

GCAC (1145)

osa-miR156f
TGACAGAAGAGAGTGA
0.75
2971

GCAC (1146)

osa-miR156g
TGACAGAAGAGAGTGA
0.75
2972

GCAC (1147)

osa-miR156h
TGACAGAAGAGAGTGA
0.75
2973

GCAC (1148)

osa-miR156i
TGACAGAAGAGAGTGA
0.75
2974

GCAC (1149)

osa-miR156j
TGACAGAAGAGAGTGA
0.75
2975

GCAC (1150)

osa-miR156k
TGACAGAAGAGAGAGA
0.85
2976

GCACA (1151)

osa-miR156l
CGACAGAAGAGAGTGA
0.75
2977

GCATA (1152)

osa-miR156m
TGACAGAAGAGAGTGA
0.75
2978

GCAC (1153)

osa-miR156n
TGACAGAAGAGAGTGA
0.75
2979

GCAC (1154)

osa-miR156o
TGACAGAAGAGAGTGA
0.7
2980

GCAT (1155)

osa-miR156p
TGACAGAAGAGAGTGA
0.7
2981

GCTC (1156)

osa-miR156q
TGACAGAACAGAGTGA
0.7
2982

GCAC (1157)

osa-miR156r
TGACAGAAGAGAGAGA
0.8
2983

GCAC (1158)

par-miR156
TGACAGAAGAGAGAGA
0.8
2984

GCAC (1159)

ppd-miR156
TGACAGAAGAGAGAGA
0.8
2985

GCAC (1160)

ppr-miR156
TGACAGAAGAGAGTGA
0.75
2986

GCAC (1161)

ppt-miR156a
TGACAGAAGAGAGTGA
0.75
2987

GCAC (1162)

ppt-miR156b
TGACAGAAGAGAGTGA
0.75
2988

GCAC (1163)

ppt-miR156c
TGACAGAAGAGAGTGA
0.75
2989

GCAC (1164)

pta-miR156b
CAGAAGATAGAGAGCA
0.95
2990

CAAC (1165)

ptc-miR156a
TGACAGAAGAGAGTGA
0.75
2991

GCAC (1166)

ptc-miR156b
TGACAGAAGAGAGTGA
0.75
2992

GCAC (1167)

ptc-miR156c
TGACAGAAGAGAGTGA
0.75
2993

GCAC (1168)

ptc-miR156d
TGACAGAAGAGAGTGA
0.75
2994

GCAC (1169)

ptc-miR156e
TGACAGAAGAGAGTGA
0.75
2995

GCAC (1170)

ptc-miR156f
TGACAGAAGAGAGTGA
0.75
2996

GCAC (1171)

ptc-miR156g
TTGACAGAAGATAGAG
0.85
2997

AGCAC (1172)

ptc-miR156h
TTGACAGAAGATAGAG
0.85
2998

AGCAC (1173)

ptc-miR156i
TTGACAGAAGATAGAG
0.85
2999

AGCAC (1174)

ptc-miR156j
TTGACAGAAGATAGAG
0.85
3000

AGCAC (1175)

ptc-miR156k
TGACAGAAGAGAGGGA
0.75
3001

GCAC (1176)

ptr-miR156
TGACAGAAGAGAGAGA
0.8
3002

GCAC (1177)

pts-miR156a
TGACAGAAGAGAGTGA
0.7
3003

GCGC (1178)

pts-miR156b
TGACAGAAGAGAGAGA
0.8
3004

GCAC (1179)

pts-miR156c
TGACAGAAGAGAGAGA
0.8
3005

GCAC (1180)

rco-miR156a
TGACAGAAGAGAGTGA
0.8
3006

GCACA (1181)

rco-miR156b
TGACAGAAGAGAGTGA
0.8
3007

GCACA (1182)

rco-miR156c
TGACAGAAGAGAGTGA
0.8
3008

GCACA (1183)

rco-miR156d
TGACAGAAGAGAGTGA
0.8
3009

GCACA (1184)

rco-miR156e
TGACAGAAGAGAGAGA
0.85
3010

GCACA (1185)

rco-miR156f
TTGACAGAAGATAGAG
0.85
3011

AGCAC (1186)

rco-miR156g
TTGACAGAAGATAGAG
0.85
3012

AGCAC (1187)

rco-miR156h
TTGACAGAAGATAGAG
0.85
3013

AGCAC (1188)

sbi-miR156a
TGACAGAAGAGAGTGA
0.75
3014

GCAC (1189)

sbi-miR156b
TGACAGAAGAGAGTGA
0.75
3015

GCAC (1190)

sbi-miR156c
TGACAGAAGAGAGTGA
0.75
3016

GCAC (1191)

sbi-miR156d
TGACAGAAGAGAGAGA
0.85
3017

GCACA (1192)

sbi-miR156e
TGACAGAAGAGAGCGA
0.75
3018

GCAC (1193)

sbi-miR156f
TGACAGAAGAGAGTGA
0.75
3019

GCAC (1194)

sbi-miR156g
TGACAGAAGAGAGTGA
0.75
3020

GCAC (1195)

sbi-miR156h
TGACAGAAGAGAGTGA
0.75
3021

GCAC (1196)

sbi-miR156i
TGACAGAAGAGAGTGA
0.75
3022

GCAC (1197)

sin-miR156
TGACAGAAGAGAGAGA
0.8
3023

GCAC (1198)

sly-miR156a
TTGACAGAAGATAGAG
0.85
3024

AGCAC (1199)

sly-miR156b
TTGACAGAAGATAGAG
0.85
3025

AGCAC (1200)

sly-miR156c
TTGACAGAAGATAGAG
0.85
3026

AGCAC (1201)

smo-miR156a
CGACAGAAGAGAGTGA
0.75
3027

GCAC (1202)

smo-miR156b
CTGACAGAAGATAGAG
0.85
3028

AGCAC (1203)

smo-miR156c
TTGACAGAAGAAAGAG
0.8
3029

AGCAC (1204)

smo-miR156d
TTGACAGAAGACAGGG
0.75
3030

AGCAC (1205)

sof-miR156
TGACAGAAGAGAGTGA
0.75
3031

GCAC (1206)

sof-miR156c
TGACAGAAGAGAGAGA
0.8
3032

GCAC (1207)

sof-miR156d
TGACAGAAGAGAGAGA
0.8
3033

GCAC (1208)

sof-miR156e
TGACAGAAGAGAGAGA
0.8
3034

GCAC (1209)

sof-miR156f
TGACAGAAGAGAGAGA
0.8
3035

GCAC (1210)

sof-miR156g
TGACAGAAGAGAGAGA
0.8
3036

GCAC (1211)

sof-miR156h
TGACAGAAGAGAGAGA
0.8
3037

GCAC (1212)

sof-miR156u
TGACAGAAGAGAGAGA
0.8
3038

GCAC (1213)

spr-miR156
TGACAGAAGAGAGAGA
0.8
3039

GCAC (1214)

ssp-miR156
TGACAGAAGAGAGTGA
0.8
3040

GCACA (1215)

stu-miR156a
TGACAGAAGAGAGTGA
0.75
3041

GCAC (1216)

stu-miR156b
TGACAGAAGAGAGAGA
0.8
3042

GCAC (1217)

stu-miR156c
TGACAGAAGAGAGAGA
0.8
3043

GCAC (1218)

stu-miR156d
TGACAGAAGAGAGAGA
0.8
3044

GCAC (1219)

stu-miR156e
TGACAGAAGAGAGAGA
0.8
3045

GCAC (1220)

tae-miR156
TGACAGAAGAGAGTGA
0.8
3046

GCACA (1221)

tae-miR156a
TGACAGAAGAGAGAGA
0.8
3047

GCAC (1222)

tae-miR156b
TGACAGAAGAGAGAGA
0.8
3048

GCAC (1223)

tcc-miR156a
TGACAGAAGAGAGAGA
0.85
3049

GCACA (1224)

tcc-miR156b
TGACAGAAGAGAGTGA
0.75
3050

GCAC (1225)

tcc-miR156c
TGACAGAAGAGAGTGA
0.75
3051

GCAC (1226)

tcc-miR156d
TGACAGAAGAGAGTGA
0.75
3052

GCAC (1227)

tcc-miR156e
TTGACAGAAGATAGAG
0.85
3053

AGCAC (1228)

tcc-miR156f
TTGACAGAAGATAGAG
0.85
3054

AGCAC (1229)

tcc-miR156g
TGACAGAAGAGAGTGA
0.75
3055

GCAC (1230)

tre-miR156
TGACAGAAGAGAGTGA
0.75
3056

GCAC (1231)

vvi-miR156a
TGACAGAAGAGAGGGA
0.75
3057

GCAC (1232)

vvi-miR156b
TGACAGAAGAGAGTGA
0.75
3058

GCAC (1233)

vvi-miR156c
TGACAGAAGAGAGTGA
0.75
3059

GCAC (1234)

vvi-miR156d
TGACAGAAGAGAGTGA
0.75
3060

GCAC (1235)

vvi-miR156e
TGACAGAGGAGAGTGA
0.7
3061

GCAC (1236)

vvi-miR156f
TTGACAGAAGATAGAG
0.85
3062

AGCAC (1237)

vvi-miR156g
TTGACAGAAGATAGAG
0.85
3063

AGCAC (1238)

vvi-miR156h
TGACAGAAGAGAGAGA
0.75
3064

GCAT (1239)

vvi-miR156i
TTGACAGAAGATAGAG
0.85
3065

AGCAC (1240)

zel-miR156
TGACAGAAGAGAGAGA
0.8
3066

GCAC (1241)

zma-miR156a
TGACAGAAGAGAGTGA
0.75
3067

GCAC (1242)

zma-miR156b
TGACAGAAGAGAGTGA
0.75
3068

GCAC (1243)

zma-miR156c
TGACAGAAGAGAGTGA
0.75
3069

GCAC (1244)

zma-miR156d
TGACAGAAGAGAGTGA
0.75
3070

GCAC (1245)

zma-miR156e
TGACAGAAGAGAGTGA
0.75
3071

GCAC (1246)

zma-miR156f
TGACAGAAGAGAGTGA
0.75
3072

GCAC (1247)

zma-miR156g
TGACAGAAGAGAGTGA
0.75
3073

GCAC (1248)

zma-miR156h
TGACAGAAGAGAGTGA
0.75
3074

GCAC (1249)

zma-miR156i
TGACAGAAGAGAGTGA
0.75
3075

GCAC (1250)

zma-miR156j
TGACAGAAGAGAGAGA
0.85
3076

GCACA (1251)

zma-miR156k
TGACAGAAGAGAGCGA
0.75
3077

GCAC (1252)

zma-miR1561
TGACAGAAGAGAGTGA
0.75
3078

GCAC (1253)

zma-miR156m
TGACAGAAGAGAGTGA
0.75
3079

GCAC (1254)

zma-miR156n
TGACAGAAGAGAGTGA
0.75
3080

GCAC (1255)

zma-miR156o
TGACAGAAGAGAGTGA
0.75
3081

GCAC (1256)

zma-miR156p
TGACAGAAGAGAGAGA
0.8
3082

GCAC (1257)

zma-miR156q
TGACAGAAGAGAGAGA
0.8
3083

GCAC (1258)

zma-miR156r
TGACAGAAGAGAGTGG
0.7
3084

GCAC (1259)

pta-
ahy-miR156a
TGACAGAAGAGAGAGA
0.8
3085

miR156b

GCAC (1260)

ahy-miR156b-5p
TTGACAGAAGATAGAG
0.85
3086

AGCAC (1261)

ahy-miR156c
TTGACAGAAGAGAGAG
0.8
3087

AGCAC (1262)

aly-miR156a
TGACAGAAGAGAGTGA
0.75
3088

GCAC (1263)

aly-miR156b
TGACAGAAGAGAGTGA
0.75
3089

GCAC (1264)

aly-miR156c
TGACAGAAGAGAGTGA
0.75
3090

GCAC (1265)

aly-miR156d
TGACAGAAGAGAGTGA
0.75
3091

GCAC (1266)

aly-miR156e
TGACAGAAGAGAGTGA
0.75
3092

GCAC (1267)

aly-miR156f
TGACAGAAGAGAGTGA
0.75
3093

GCAC (1268)

aly-miR156g
CGACAGAAGAGAGTGA
0.75
3094

GCAC (1269)

aly-miR156h
TGACAGAAGAAAGAGA
0.8
3095

GCAC (1270)

aqc-miR156a
TGACAGAAGATAGAGA
0.85
3096

GCAC (1271)

aqc-miR156b
TGACAGAAGATAGAGA
0.85
3097

GCAC (1272)

ath-miR156a
TGACAGAAGAGAGTGA
0.75
3098

GCAC (1273)

ath-miR156b
TGACAGAAGAGAGTGA
0.75
3099

GCAC (1274)

ath-miR156c
TGACAGAAGAGAGTGA
0.75
3100

GCAC (1275)

ath-miR156d
TGACAGAAGAGAGTGA
0.75
3101

GCAC (1276)

ath-miR156e
TGACAGAAGAGAGTGA
0.75
3102

GCAC (1277)

ath-miR156f
TGACAGAAGAGAGTGA
0.75
3103

GCAC (1278)

ath-miR156g
CGACAGAAGAGAGTGA
0.75
3104

GCAC (1279)

ath-miR156h
TGACAGAAGAAAGAGA
0.8
3105

GCAC (1280)

ath-miR156m
TGACAGAAGAGAGAGA
0.8
3106

GCAC (1281)

ath-miR156o
TGACAGAAGAGAGAGA
0.8
3107

GCAC (1282)

ath-miR156p
TGACAGAAGAGAGAGA
0.8
3108

GCAC (1283)

ath-miR156q
TGACAGAAGAGAGAGA
0.8
3109

GCAC (1284)

ath-miR156r
TGACAGAAGAGAGAGA
0.8
3110

GCAC (1285)

ath-miR156s
TGACAGAAGAGAGAGA
0.8
3111

GCAC (1286)

bdi-miR156
TGACAGAAGAGAGAGA
0.85
3112

GCACA (1287)

bdi-miR156b
TGACAGAAGAGAGTGA
0.75
3113

GCAC (1288)

bdi-miR156c
TGACAGAAGAGAGTGA
0.75
3114

GCAC (1289)

bdi-miR156d
TGACAGAAGAGAGTGA
0.75
3115

GCAC (1290)

bna-miR156a
TGACAGAAGAGAGTGA
0.8
3116

GCACA (1291)

bna-miR156b
TTGACAGAAGATAGAG
0.85
3117

AGCAC (1292)

bna-miR156c
TTGACAGAAGATAGAG
0.85
3118

AGCAC (1293)

can-miR156a
TGACAGAAGAGAGAGA
0.8
3119

GCAC (1294)

can-miR156b
TGACAGAAGAGAGGGA
0.75
3120

GCAC (1295)

cpt-miR156a
TGACAGAAGAGAGTGA
0.75
3121

GCAC (1296)

cpt-miR156b
TGACAGAAGAGAGAGA
0.8
3122

GCAC (1297)

cru-miR156
TGACAGAAGAGAGAGA
0.8
3123

GCAC (1298)

csi-miR156
TGACAGAAGAGAGTGA
0.75
3124

GCAC (1299)

csi-miR156a
TGACAGAAGAGAGAGA
0.8
3125

GCAC (1300)

csi-miR156b
TGACAGAAGAGAGAGA
0.8
3126

GCAC (1301)

ctr-miR156
TGACAGAAGAGAGTGA
0.75
3127

GCAC (1302)

eca-miR156
TGACAGAAGAGAGAGA
0.8
3128

GCAC (1303)

far-miR156a
TGACAGAAGAGAGAGA
0.85
3129

GCACA (1304)

far-miR156b
TTGACAGAAGAGAGAG
0.8
3130

AGCAC (1305)

ghr-miR156a
TGACAGAAGAGAGTGA
0.75
3131

GCAC (1306)

ghr-miR156b
TGACAGAAGAGAGTGA
0.75
3132

GCAC (1307)

ghr-miR156c
TGTCAGAAGAGAGTGA
0.75
3133

GCAC (1308)

ghr-miR156d
TGACAGAAGAGAGTGA
0.75
3134

GCAC (1309)

gma-miR156a
TGACAGAAGAGAGTGA
0.75
3135

GCAC (1310)

gma-miR156b
TGACAGAAGAGAGAGA
0.85
3136

GCACA (1311)

gma-miR156c
TTGACAGAAGATAGAG
0.85
3137

AGCAC (1312)

gma-miR156d
TTGACAGAAGATAGAG
0.85
3138

AGCAC (1313)

gma-miR156e
TTGACAGAAGATAGAG
0.85
3139

AGCAC (1314)

gma-miR156f
TTGACAGAAGAGAGAG
0.85
3140

AGCACA (1315)

gma-miR156g
ACAGAAGATAGAGAGC
0.9
3141

ACAG (1316)

gma-miR156h
TGACAGAAGAGAGAGA
0.8
3142

GCAC (1317)

gma-miR156i
TGACAGAAGAGAGAGA
0.8
3143

GCAC (1318)

han-miR156
TGACAGAAGAGAGAGA
0.8
3144

GCAC (1319)

hvs-miR156
TGACAGAAGAGAGAGA
0.8
3145

GCAC (1320)

hvu-miR156
TGACAGAAGAGAGTGA
0.8
3146

GCACA (1321)

hvv-miR156a
TGACAGAAGAGAGTGA
0.75
3147

GCAC (1322)

hvv-miR156b
TGACAGAAGAGAGAGA
0.8
3148

GCAC (1323)

hvv-miR156c
TGACAGAAGAGAGAGA
0.8
3149

GCAC (1324)

hvv-miR156d
TGACAGAAGAGAGAGA
0.8
3150

GCAC (1325)

lja-miR156
TGACAGAAGAGAGAGA
0.8
3151

GCAC (1326)

lsa-miR156
TGACAGAAGAGAGAGA
0.8
3152

GCAC (1327)

mdo-miR156a
TGACAGAAGAGAGAGA
0.8
3153

GCAC (1328)

mdo-miR156b
TGACAGAAGAGAGAGA
0.8
3154

GCAC (1329)

mtr-miR156
TGACAGAAGAGAGAGA
0.85
3155

GCACA (1330)

mtr-miR156b
TGACAGAAGAGAGTGA
0.75
3156

GCAC (1331)

mtr-miR156c
TGACAGAAGAGAGTGA
0.75
3157

GCAC (1332)

mtr-miR156d
TGACAGAAGAGAGTGA
0.75
3158

GCAC (1333)

mtr-miR156e
TTGACAGAAGATAGAG
0.85
3159

AGCAC (1334)

mtr-miR156f
TTGACAGAAGATAGAG
0.85
3160

AGCAC (1335)

mtr-miR156g
TTGACAGAAGATAGAG
0.8
3161

GGCAC (1336)

mtr-miR156h
TTGACAGAAGATAGAG
0.85
3162

AGCAC (1337)

mtr-miR156i
TGACAGAAGAGAGTGA
0.75
3163

GCAC (1338)

nbe-miR156a
TGACAGAAGAGAGAGA
0.8
3164

GCAC (1339)

nbe-miR156b
TGACAGAAGAGAGAGA
0.8
3165

GCAC (1340)

oru-miR156
TGACAGAAGAGAGTGA
0.75
3166

GCAC (1341)

osa-miR156a
TGACAGAAGAGAGTGA
0.75
3167

GCAC (1342)

osa-miR156b
TGACAGAAGAGAGTGA
0.75
3168

GCAC (1343)

osa-miR156c
TGACAGAAGAGAGTGA
0.75
3169

GCAC (1344)

osa-miR156d
TGACAGAAGAGAGTGA
0.75
3170

GCAC (1345)

osa-miR156e
TGACAGAAGAGAGTGA
0.75
3171

GCAC (1346)

osa-miR156f
TGACAGAAGAGAGTGA
0.75
3172

GCAC (1347)

osa-miR156g
TGACAGAAGAGAGTGA
0.75
3173

GCAC (1348)

osa-miR156h
TGACAGAAGAGAGTGA
0.75
3174

GCAC (1349)

osa-miR156i
TGACAGAAGAGAGTGA
0.75
3175

GCAC (1350)

osa-miR156j
TGACAGAAGAGAGTGA
0.75
3176

GCAC (1351)

osa-miR156k
TGACAGAAGAGAGAGA
0.85
3177

GCACA (1352)

osa-miR156l
CGACAGAAGAGAGTGA
0.75
3178

GCATA (1353)

osa-miR156m
TGACAGAAGAGAGTGA
0.75
3179

GCAC (1354)

osa-miR156n
TGACAGAAGAGAGTGA
0.75
3180

GCAC (1355)

osa-miR156o
TGACAGAAGAGAGTGA
0.7
3181

GCAT (1356)

osa-miR156p
TGACAGAAGAGAGTGA
0.7
3182

GCTC (1357)

osa-miR156q
TGACAGAACAGAGTGA
0.7
3183

GCAC (1358)

osa-miR156r
TGACAGAAGAGAGAGA
0.8
3184

GCAC (1359)

par-miR156
TGACAGAAGAGAGAGA
0.8
3185

GCAC (1360)

ppd-miR156
TGACAGAAGAGAGAGA
0.8
3186

GCAC (1361)

ppr-miR156
TGACAGAAGAGAGTGA
0.75
3187

GCAC (1362)

ppt-miR156a
TGACAGAAGAGAGTGA
0.75
3188

GCAC (1363)

ppt-miR156b
TGACAGAAGAGAGTGA
0.75
3189

GCAC (1364)

ppt-miR156c
TGACAGAAGAGAGTGA
0.75
3190

GCAC (1365)

pta-miR156a
CAGAAGATAGAGAGCA
0.95
3191

CATC (1366)

ptc-miR156a
TGACAGAAGAGAGTGA
0.75
3192

GCAC (1367)

ptc-miR156b
TGACAGAAGAGAGTGA
0.75
3193

GCAC (1368)

ptc-miR156c
TGACAGAAGAGAGTGA
0.75
3194

GCAC (1369)

ptc-miR156d
TGACAGAAGAGAGTGA
0.75
3195

GCAC (1370)

ptc-miR156e
TGACAGAAGAGAGTGA
0.75
3196

GCAC (1371)

ptc-miR156f
TGACAGAAGAGAGTGA
0.75
3197

GCAC (1372)

ptc-miR156g
TTGACAGAAGATAGAG
0.85
3198

AGCAC (1373)

ptc-miR156h
TTGACAGAAGATAGAG
0.85
3199

AGCAC (1374)

ptc-miR156i
TTGACAGAAGATAGAG
0.85
3200

AGCAC (1375)

ptc-miR156j
TTGACAGAAGATAGAG
0.85
3201

AGCAC (1376)

ptc-miR156k
TGACAGAAGAGAGGGA
0.75
3202

GCAC (1377)

ptr-miR156
TGACAGAAGAGAGAGA
0.8
3203

GCAC (1378)

pts-miR156a
TGACAGAAGAGAGTGA
0.7
3204

GCGC (1379)

pts-miR156b
TGACAGAAGAGAGAGA
0.8
3205

GCAC (1380)

pts-miR156c
TGACAGAAGAGAGAGA
0.8
3206

GCAC (1381)

rco-miR156a
TGACAGAAGAGAGTGA
0.8
3207

GCACA (1382)

rco-miR156b
TGACAGAAGAGAGTGA
0.8
3208

GCACA (1383)

rco-miR156c
TGACAGAAGAGAGTGA
0.8
3209

GCACA (1384)

rco-miR156d
TGACAGAAGAGAGTGA
0.8
3210

GCACA (1385)

rco-miR156e
TGACAGAAGAGAGAGA
0.85
3211

GCACA (1386)

rco-miR156f
TTGACAGAAGATAGAG
0.85
3212

AGCAC (1387)

rco-miR156g
TTGACAGAAGATAGAG
0.85
3213

AGCAC (1388)

rco-miR156h
TTGACAGAAGATAGAG
0.85
3214

AGCAC (1389)

sbi-miR156a
TGACAGAAGAGAGTGA
0.75
3215

GCAC (1390)

sbi-miR156b
TGACAGAAGAGAGTGA
0.75
3216

GCAC (1391)

sbi-miR156c
TGACAGAAGAGAGTGA
0.75
3217

GCAC (1392)

sbi-miR156d
TGACAGAAGAGAGAGA
0.85
3218

GCACA (1393)

sbi-miR156e
TGACAGAAGAGAGCGA
0.75
3219

GCAC (1394)

sbi-miR156f
TGACAGAAGAGAGTGA
0.75
3220

GCAC (1395)

sbi-miR156g
TGACAGAAGAGAGTGA
0.75
3221

GCAC (1396)

sbi-miR156h
TGACAGAAGAGAGTGA
0.75
3222

GCAC (1397)

sbi-miR156i
TGACAGAAGAGAGTGA
0.75
3223

GCAC (1398)

sin-miR156
TGACAGAAGAGAGAGA
0.8
3224

GCAC (1399)

sly-miR156a
TTGACAGAAGATAGAG
0.85
3225

AGCAC (1400)

sly-miR156b
TTGACAGAAGATAGAG
0.85
3226

AGCAC (1401)

sly-miR156c
TTGACAGAAGATAGAG
0.85
3227

AGCAC (1402)

smo-miR156a
CGACAGAAGAGAGTGA
0.75
3228

GCAC (1403)

smo-miR156b
CTGACAGAAGATAGAG
0.85
3229

AGCAC (1404)

smo-miR156c
TTGACAGAAGAAAGAG
0.8
3230

AGCAC (1405)

smo-miR156d
TTGACAGAAGACAGGG
0.75
3231

AGCAC (1406)

sof-miR156
TGACAGAAGAGAGTGA
0.75
3232

GCAC (1407)

sof-miR156c
TGACAGAAGAGAGAGA
0.8
3233

GCAC (1408)

sof-miR156d
TGACAGAAGAGAGAGA
0.8
3234

GCAC (1409)

sof-miR156e
TGACAGAAGAGAGAGA
0.8
3235

GCAC (1410)

sof-miR156f
TGACAGAAGAGAGAGA
0.8
3236

GCAC (1411)

sof-miR156g
TGACAGAAGAGAGAGA
0.8
3237

GCAC (1412)

sof-miR156h
TGACAGAAGAGAGAGA
0.8
3238

GCAC (1413)

sof-miR156u
TGACAGAAGAGAGAGA
0.8
3239

GCAC (1414)

spr-miR156
TGACAGAAGAGAGAGA
0.8
3240

GCAC (1415)

ssp-miR156
TGACAGAAGAGAGTGA
0.8
3241

GCACA (1416)

stu-miR156a
TGACAGAAGAGAGTGA
0.75
3242

GCAC (1417)

stu-miR156b
TGACAGAAGAGAGAGA
0.8
3243

GCAC (1418)

stu-miR156c
TGACAGAAGAGAGAGA
0.8
3244

GCAC (1419)

stu-miR156d
TGACAGAAGAGAGAGA
0.8
3245

GCAC (1420)

stu-miR156e
TGACAGAAGAGAGAGA
0.8
3246

GCAC (1421)

tae-miR156
TGACAGAAGAGAGTGA
0.8
3247

GCACA (1422)

tae-miR156a
TGACAGAAGAGAGAGA
0.8
3248

GCAC (1423)

tae-miR156b
TGACAGAAGAGAGAGA
0.8
3249

GCAC (1424)

tcc-miR156a
TGACAGAAGAGAGAGA
0.85
3250

GCACA (1425)

tcc-miR156b
TGACAGAAGAGAGTGA
0.75
3251

GCAC (1426)

tcc-miR156c
TGACAGAAGAGAGTGA
0.75
3252

GCAC (1427)

tcc-miR156d
TGACAGAAGAGAGTGA
0.75
3253

GCAC (1428)

tcc-miR156e
TTGACAGAAGATAGAG
0.85
3254

AGCAC (1429)

tcc-miR156f
TTGACAGAAGATAGAG
0.85
3255

AGCAC (1430)

tcc-miR156g
TGACAGAAGAGAGTGA
0.75
3256

GCAC (1431)

tre-miR156
TGACAGAAGAGAGTGA
0.75
3257

GCAC (1432)

vvi-miR156a
TGACAGAAGAGAGGGA
0.75
3258

GCAC (1433)

vvi-miR156b
TGACAGAAGAGAGTGA
0.75
3259

GCAC (1434)

vvi-miR156c
TGACAGAAGAGAGTGA
0.75
3260

GCAC (1435)

vvi-miR156d
TGACAGAAGAGAGTGA
0.75
3261

GCAC (1436)

vvi-miR156e
TGACAGAGGAGAGTGA
0.7
3262

GCAC (1437)

vvi-miR156f
TTGACAGAAGATAGAG
0.85
3263

AGCAC (1438)

vvi-miR156g
TTGACAGAAGATAGAG
0.85
3264

AGCAC (1439)

vvi-miR156h
TGACAGAAGAGAGAGA
0.75
3265

GCAT (1440)

vvi-miR156i
TTGACAGAAGATAGAG
0.85
3266

AGCAC (1441)

zel-miR156
TGACAGAAGAGAGAGA
0.8
3267

GCAC (1442)

zma-miR156a
TGACAGAAGAGAGTGA
0.75
3268

GCAC (1443)

zma-miR156b
TGACAGAAGAGAGTGA
0.75
3269

GCAC (1444)

zma-miR156c
TGACAGAAGAGAGTGA
0.75
3270

GCAC (1445)

zma-miR156d
TGACAGAAGAGAGTGA
0.75
3271

GCAC (1446)

zma-miR156e
TGACAGAAGAGAGTGA
0.75
3272

GCAC (1447)

zma-miR156f
TGACAGAAGAGAGTGA
0.75
3273

GCAC (1448)

zma-miR156g
TGACAGAAGAGAGTGA
0.75
3274

GCAC (1449)

zma-miR156h
TGACAGAAGAGAGTGA
0.75
3275

GCAC (1450)

zma-miR156i
TGACAGAAGAGAGTGA
0.75
3276

GCAC (1451)

zma-miR156j
TGACAGAAGAGAGAGA
0.85
3277

GCACA (1452)

zma-miR156k
TGACAGAAGAGAGCGA
0.75
3278

GCAC (1453)

zma-miR156l
TGACAGAAGAGAGTGA
0.75
3279

GCAC (1454)

zma-miR156m
TGACAGAAGAGAGTGA
0.75
3280

GCAC (1455)

zma-miR156n
TGACAGAAGAGAGTGA
0.75
3281

GCAC (1456)

zma-miR156o
TGACAGAAGAGAGTGA
0.75
3282

GCAC (1457)

zma-miR156p
TGACAGAAGAGAGAGA
0.8
3283

GCAC (1458)

zma-miR156q
TGACAGAAGAGAGAGA
0.8
3284

GCAC (1459)

zma-miR156r
TGACAGAAGAGAGTGG
0.7
3285

GCAC (1460)

ptc-
ahy-miR159
TTTGGATTGAAGGGAGC
0.95
3286

miRf10271-

TCTA (1461)

akr

aly-miR159a
TTTGGATTGAAGGGAGC
0.95
3287

TCTA (1462)

aly-miR159b
TTTGGATTGAAGGGAGC
0.9
3288

TCTT (1463)

aqc-miR159
TTTGGACTGAAGGGAGC
0.9
3289

TCTA (1464)

ath-miR159a
TTTGGATTGAAGGGAGC
0.95
3290

TCTA (1465)

ath-miR159b
TTTGGATTGAAGGGAGC
0.9
3291

TCTT (1466)

bdi-miR159
CTTGGATTGAAGGGAGC
0.9
3292

TCT (1467)

bna-miR159
TTTGGATTGAAGGGAGC
0.95
3293

TCTA (1468)

bra-miR159a
TTTGGATTGAAGGGAGC
0.95
3294

TCTA (1469)

csi-miR159
TTTGGATTGAAGGGAGC
0.95
3295

TCTA (1470)

far-miR159
TTTGGATTGAAGGGAGC
0.9
3296

TCTG (1471)

gma-miR159a-3p
TTTGGATTGAAGGGAGC
0.95
3297

TCTA (1472)

hvu-miR159a
TTTGGATTGAAGGGAGC
0.9
3298

TCTG (1473)

hvu-miR159b
TTTGGATTGAAGGGAGC
0.9
3299

TCTG (1474)

mtr-miR159a
TTTGGATTGAAGGGAGC
0.95
3300

TCTA (1475)

osa-miR159a.1
TTTGGATTGAAGGGAGC
0.9
3301

TCTG (1476)

osa-miR159b
TTTGGATTGAAGGGAGC
0.9
3302

TCTG (1477)

osa-miR159c
ATTGGATTGAAGGGAG
0.9
3303

CTCCA (1478)

osa-miR159f
CTTGGATTGAAGGGAGC
0.95
3304

TCTA (1479)

pta-miR159a
TTGGATTGAAGGGAGCT
0.9
3305

CCA (1480)

ptc-miR159a
TTTGGATTGAAGGGAGC
0.95
3306

TCTA (1481)

ptc-miR159b
TTTGGATTGAAGGGAGC
0.95
3307

TCTA (1482)

ptc-miR159c
TTTGGATTGAAGGGAGC
0.95
3308

TCTA (1483)

pvu-miR159a.1
TTTGGATTGAAGGGAGC
0.95
3309

TCTA (1484)

rco-miR159
TTTGGATTGAAGGGAGC
0.95
3310

TCTA (1485)

sbi-miR159a
TTTGGATTGAAGGGAGC
0.9
3311

TCTG (1486)

sly-miR159
TTTGGATTGAAGGGAGC
0.95
3312

TCTA (1487)

sof-miR159a
TTTGGATTGAAGGGAGC
0.9
3313

TCTG (1488)

sof-miR159b
TTTGGATTGAAGGGAGC
0.9
3314

TCTG (1489)

sof-miR159d
TTTGGATTGAAGGGAGC
0.9
3315

TCTG (1490)

ssp-miR159a
TTTGGATTGAAGGGAGC
0.9
3316

TCTG (1491)

tae-miR159a
TTTGGATTGAAGGGAGC
0.9
3317

TCTG (1492)

tae-miR159b
TTTGGATTGAAGGGAGC
0.9
3318

TCTG (1493)

vvi-miR159c
TTTGGATTGAAGGGAGC
0.95
3319

TCTA (1494)

zma-miR159a
TTTGGATTGAAGGGAGC
0.9
3320

TCTG (1495)

zma-miR159b
TTTGGATTGAAGGGAGC
0.9
3321

TCTG (1496)

zma-miR159f
TTTGGATTGAAGGGAGC
0.9
3322

TCTG (1497)

zma-miR159j
TTTGGATTGAAGGGAGC
0.9
3323

TCTG (1498)

zma-miR159k
TTTGGATTGAAGGGAGC
0.9
3324

TCTG (1499)

ptc-
gma-miR156g
ACAGAAGATAGAGAGC
0.9
3325

miRf10985-

ACAG (1500)

akr

ath-
ahy-miR156a
TGACAGAAGAGAGAGA
0.9
3326

miR157a

GCAC (1501)

ahy-miR156b-5p
TTGACAGAAGATAGAG
1
3327

AGCAC (1502)

ahy-miR156c
TTGACAGAAGAGAGAG
0.95
3328

AGCAC (1503)

aly-miR156a
TGACAGAAGAGAGTGA
0.86
3329

GCAC (1504)

aly-miR156b
TGACAGAAGAGAGTGA
0.86
3330

GCAC (1505)

aly-miR156c
TGACAGAAGAGAGTGA
0.86
3331

GCAC (1506)

aly-miR156d
TGACAGAAGAGAGTGA
0.86
3332

GCAC (1507)

aly-miR156e
TGACAGAAGAGAGTGA
0.86
3333

GCAC (1508)

aly-miR156f
TGACAGAAGAGAGTGA
0.86
3334

GCAC (1509)

aly-miR156g
CGACAGAAGAGAGTGA
0.81
3335

GCAC (1510)

aly-miR156h
TGACAGAAGAAAGAGA
0.9
3336

GCAC (1511)

aqc-miR156a
TGACAGAAGATAGAGA
0.95
3337

GCAC (1512)

aqc-miR156b
TGACAGAAGATAGAGA
0.95
3338

GCAC (1513)

ath-miR156a
TGACAGAAGAGAGTGA
0.86
3339

GCAC (1514)

ath-miR156b
TGACAGAAGAGAGTGA
0.86
3340

GCAC (1515)

ath-miR156c
TGACAGAAGAGAGTGA
0.86
3341

GCAC (1516)

ath-miR156d
TGACAGAAGAGAGTGA
0.86
3342

GCAC (1517)

ath-miR156e
TGACAGAAGAGAGTGA
0.86
3343

GCAC (1518)

ath-miR156f
TGACAGAAGAGAGTGA
0.86
3344

GCAC (1519)

ath-miR156g
CGACAGAAGAGAGTGA
0.81
3345

GCAC (1520)

ath-miR156h
TGACAGAAGAAAGAGA
0.9
3346

GCAC (1521)

ath-miR156m
TGACAGAAGAGAGAGA
0.9
3347

GCAC (1522)

ath-miR156o
TGACAGAAGAGAGAGA
0.9
3348

GCAC (1523)

ath-miR156p
TGACAGAAGAGAGAGA
0.9
3349

GCAC (1524)

ath-miR156q
TGACAGAAGAGAGAGA
0.9
3350

GCAC (1525)

ath-miR156r
TGACAGAAGAGAGAGA
0.9
3351

GCAC (1526)

ath-miR156s
TGACAGAAGAGAGAGA
0.9
3352

GCAC (1527)

bdi-miR156
TGACAGAAGAGAGAGA
0.9
3353

GCACA (1528)

bdi-miR156b
TGACAGAAGAGAGTGA
0.86
3354

GCAC (1529)

bdi-miR156c
TGACAGAAGAGAGTGA
0.86
3355

GCAC (1530)

bdi-miR156d
TGACAGAAGAGAGTGA
0.86
3356

GCAC (1531)

bna-miR156a
TGACAGAAGAGAGTGA
0.86
3357

GCACA (1532)

bna-miR156b
TTGACAGAAGATAGAG
1
3358

AGCAC (1533)

bna-miR156c
TTGACAGAAGATAGAG
1
3359

AGCAC (1534)

can-miR156a
TGACAGAAGAGAGAGA
0.9
3360

GCAC (1535)

can-miR156b
TGACAGAAGAGAGGGA
0.86
3361

GCAC (1536)

cpt-miR156a
TGACAGAAGAGAGTGA
0.86
3362

GCAC (1537)

cpt-miR156b
TGACAGAAGAGAGAGA
0.9
3363

GCAC (1538)

cru-miR156
TGACAGAAGAGAGAGA
0.9
3364

GCAC (1539)

csi-miR156
TGACAGAAGAGAGTGA
0.86
3365

GCAC (1540)

csi-miR156a
TGACAGAAGAGAGAGA
0.9
3366

GCAC (1541)

csi-miR156b
TGACAGAAGAGAGAGA
0.9
3367

GCAC (1542)

ctr-miR156
TGACAGAAGAGAGTGA
0.86
3368

GCAC (1543)

eca-miR156
TGACAGAAGAGAGAGA
0.9
3369

GCAC (1544)

far-miR156a
TGACAGAAGAGAGAGA
0.9
3370

GCACA (1545)

far-miR156b
TTGACAGAAGAGAGAG
0.95
3371

AGCAC (1546)

ghr-miR156a
TGACAGAAGAGAGTGA
0.86
3372

GCAC (1547)

ghr-miR156b
TGACAGAAGAGAGTGA
0.86
3373

GCAC (1548)

ghr-miR156c
TGTCAGAAGAGAGTGA
0.81
3374

GCAC (1549)

ghr-miR156d
TGACAGAAGAGAGTGA
0.86
3375

GCAC (1550)

gma-miR156a
TGACAGAAGAGAGTGA
0.86
3376

GCAC (1551)

gma-miR156b
TGACAGAAGAGAGAGA
0.9
3377

GCACA (1552)

gma-miR156c
TTGACAGAAGATAGAG
1
3378

AGCAC (1553)

gma-miR156d
TTGACAGAAGATAGAG
1
3379

AGCAC (1554)

gma-miR156e
TTGACAGAAGATAGAG
1
3380

AGCAC (1555)

gma-miR156f
TTGACAGAAGAGAGAG
0.95
3381

AGCACA (1556)

gma-miR156g
ACAGAAGATAGAGAGC
0.86
3382

ACAG (1557)

gma-miR156h
TGACAGAAGAGAGAGA
0.9
3383

GCAC (1558)

gma-miR156i
TGACAGAAGAGAGAGA
0.9
3384

GCAC (1559)

han-miR156
TGACAGAAGAGAGAGA
0.9
3385

GCAC (1560)

hvs-miR156
TGACAGAAGAGAGAGA
0.9
3386

GCAC (1561)

hvu-miR156
TGACAGAAGAGAGTGA
0.86
3387

GCACA (1562)

hvv-miR156a
TGACAGAAGAGAGTGA
0.86
3388

GCAC (1563)

hvv-miR156b
TGACAGAAGAGAGAGA
0.9
3389

GCAC (1564)

hvv-miR156c
TGACAGAAGAGAGAGA
0.9
3390

GCAC (1565)

hvv-miR156d
TGACAGAAGAGAGAGA
0.9
3391

GCAC (1566)

lja-miR156
TGACAGAAGAGAGAGA
0.9
3392

GCAC (1567)

lsa-miR156
TGACAGAAGAGAGAGA
0.9
3393

GCAC (1568)

mdo-miR156a
TGACAGAAGAGAGAGA
0.9
3394

GCAC (1569)

mdo-miR156b
TGACAGAAGAGAGAGA
0.9
3395

GCAC (1570)

mtr-miR156
TGACAGAAGAGAGAGA
0.9
3396

GCACA (1571)

mtr-miR156b
TGACAGAAGAGAGTGA
0.86
3397

GCAC (1572)

mtr-miR156c
TGACAGAAGAGAGTGA
0.86
3398

GCAC (1573)

mtr-miR156d
TGACAGAAGAGAGTGA
0.86
3399

GCAC (1574)

mtr-miR156e
TTGACAGAAGATAGAG
1
3400

AGCAC (1575)

mtr-miR156f
TTGACAGAAGATAGAG
1
3401

AGCAC (1576)

mtr-miR156g
TTGACAGAAGATAGAG
0.95
3402

GGCAC (1577)

mtr-miR156h
TTGACAGAAGATAGAG
1
3403

AGCAC (1578)

mtr-miR156i
TGACAGAAGAGAGTGA
0.86
3404

GCAC (1579)

nbe-miR156a
TGACAGAAGAGAGAGA
0.9
3405

GCAC (1580)

nbe-miR156b
TGACAGAAGAGAGAGA
0.9
3406

GCAC (1581)

oru-miR156
TGACAGAAGAGAGTGA
0.86
3407

GCAC (1582)

osa-miR156a
TGACAGAAGAGAGTGA
0.86
3408

GCAC (1583)

osa-miR156b
TGACAGAAGAGAGTGA
0.86
3409

GCAC (1584)

osa-miR156c
TGACAGAAGAGAGTGA
0.86
3410

GCAC (1585)

osa-miR156d
TGACAGAAGAGAGTGA
0.86
3411

GCAC (1586)

osa-miR156e
TGACAGAAGAGAGTGA
0.86
3412

GCAC (1587)

osa-miR156f
TGACAGAAGAGAGTGA
0.86
3413

GCAC (1588)

osa-miR156g
TGACAGAAGAGAGTGA
0.86
3414

GCAC (1589)

osa-miR156h
TGACAGAAGAGAGTGA
0.86
3415

GCAC (1590)

osa-miR156i
TGACAGAAGAGAGTGA
0.86
3416

GCAC (1591)

osa-miR156j
TGACAGAAGAGAGTGA
0.86
3417

GCAC (1592)

osa-miR156k
TGACAGAAGAGAGAGA
0.9
3418

GCACA (1593)

osa-miR156l
CGACAGAAGAGAGTGA
0.76
3419

GCATA (1594)

osa-miR156m
TGACAGAAGAGAGTGA
0.86
3420

GCAC (1595)

osa-miR156n
TGACAGAAGAGAGTGA
0.86
3421

GCAC (1596)

osa-miR156o
TGACAGAAGAGAGTGA
0.81
3422

GCAT (1597)

osa-miR156p
TGACAGAAGAGAGTGA
0.81
3423

GCTC (1598)

osa-miR156q
TGACAGAACAGAGTGA
0.81
3424

GCAC (1599)

osa-miR156r
TGACAGAAGAGAGAGA
0.9
3425

GCAC (1600)

par-miR156
TGACAGAAGAGAGAGA
0.9
3426

GCAC (1601)

pga-miR156a
GATCCTAGAGCCCTTGA
0.38
3427

GCC (1602)

ppd-miR156
TGACAGAAGAGAGAGA
0.9
3428

GCAC (1603)

ppr-miR156
TGACAGAAGAGAGTGA
0.86
3429

GCAC (1604)

ppt-miR156a
TGACAGAAGAGAGTGA
0.86
3430

GCAC (1605)

ppt-miR156b
TGACAGAAGAGAGTGA
0.86
3431

GCAC (1606)

ppt-miR156c
TGACAGAAGAGAGTGA
0.86
3432

GCAC (1607)

pta-miR156a
CAGAAGATAGAGAGCA
0.81
3433

CATC (1608)

pta-miR156b
CAGAAGATAGAGAGCA
0.81
3434

CAAC (1609)

ptc-miR156a
TGACAGAAGAGAGTGA
0.86
3435

GCAC (1610)

ptc-miR156b
TGACAGAAGAGAGTGA
0.86
3436

GCAC (1611)

ptc-miR156c
TGACAGAAGAGAGTGA
0.86
3437

GCAC (1612)

ptc-miR156d
TGACAGAAGAGAGTGA
0.86
3438

GCAC (1613)

ptc-miR156e
TGACAGAAGAGAGTGA
0.86
3439

GCAC (1614)

ptc-miR156f
TGACAGAAGAGAGTGA
0.86
3440

GCAC (1615)

ptc-miR156g
TTGACAGAAGATAGAG
1
3441

AGCAC (1616)

ptc-miR156h
TTGACAGAAGATAGAG
1
3442

AGCAC (1617)

ptc-miR156i
TTGACAGAAGATAGAG
1
3443

AGCAC (1618)

ptc-miR156j
TTGACAGAAGATAGAG
1
3444

AGCAC (1619)

ptc-miR156k
TGACAGAAGAGAGGGA
0.86
3445

GCAC (1620)

ptr-miR156
TGACAGAAGAGAGAGA
0.9
3446

GCAC (1621)

pts-miR156a
TGACAGAAGAGAGTGA
0.81
3447

GCGC (1622)

pts-miR156b
TGACAGAAGAGAGAGA
0.9
3448

GCAC (1623)

pts-miR156c
TGACAGAAGAGAGAGA
0.9
3449

GCAC (1624)

rco-miR156a
TGACAGAAGAGAGTGA
0.86
3450

GCACA (1625)

rco-miR156b
TGACAGAAGAGAGTGA
0.86
3451

GCACA (1626)

rco-miR156c
TGACAGAAGAGAGTGA
0.86
3452

GCACA (1627)

rco-miR156d
TGACAGAAGAGAGTGA
0.86
3453

GCACA (1628)

rco-miR156e
TGACAGAAGAGAGAGA
0.9
3454

GCACA (1629)

rco-miR156f
TTGACAGAAGATAGAG
1
3455

AGCAC (1630)

rco-miR156g
TTGACAGAAGATAGAG
1
3456

AGCAC (1631)

rco-miR156h
TTGACAGAAGATAGAG
1
3457

AGCAC (1632)

sbi-miR156a
TGACAGAAGAGAGTGA
0.86
3458

GCAC (1633)

sbi-miR156b
TGACAGAAGAGAGTGA
0.86
3459

GCAC (1634)

sbi-miR156c
TGACAGAAGAGAGTGA
0.86
3460

GCAC (1635)

sbi-miR156d
TGACAGAAGAGAGAGA
0.9
3461

GCACA (1636)

sbi-miR156e
TGACAGAAGAGAGCGA
0.86
3462

GCAC (1637)

sbi-miR156f
TGACAGAAGAGAGTGA
0.86
3463

GCAC (1638)

sbi-miR156g
TGACAGAAGAGAGTGA
0.86
3464

GCAC (1639)

sbi-miR156h
TGACAGAAGAGAGTGA
0.86
3465

GCAC (1640)

sbi-miR156i
TGACAGAAGAGAGTGA
0.86
3466

GCAC (1641)

sin-miR156
TGACAGAAGAGAGAGA
0.9
3467

GCAC (1642)

sly-miR156a
TTGACAGAAGATAGAG
1
3468

AGCAC (1643)

sly-miR156b
TTGACAGAAGATAGAG
1
3469

AGCAC (1644)

sly-miR156c
TTGACAGAAGATAGAG
1
3470

AGCAC (1645)

smo-miR156a
CGACAGAAGAGAGTGA
0.81
3471

GCAC (1646)

smo-miR156b
CTGACAGAAGATAGAG
0.95
3472

AGCAC (1647)

smo-miR156c
TTGACAGAAGAAAGAG
0.95
3473

AGCAC (1648)

smo-miR156d
TTGACAGAAGACAGGG
0.9
3474

AGCAC (1649)

sof-miR156
TGACAGAAGAGAGTGA
0.86
3475

GCAC (1650)

sof-miR156c
TGACAGAAGAGAGAGA
0.9
3476

GCAC (1651)

sof-miR156d
TGACAGAAGAGAGAGA
0.9
3477

GCAC (1652)

sof-miR156e
TGACAGAAGAGAGAGA
0.9
3478

GCAC (1653)

sof-miR156f
TGACAGAAGAGAGAGA
0.9
3479

GCAC (1654)

sof-miR156g
TGACAGAAGAGAGAGA
0.9
3480

GCAC (1655)

sof-miR156h
TGACAGAAGAGAGAGA
0.9
3481

GCAC (1656)

sof-miR156u
TGACAGAAGAGAGAGA
0.9
3482

GCAC (1657)

spr-miR156
TGACAGAAGAGAGAGA
0.9
3483

GCAC (1658)

ssp-miR156
TGACAGAAGAGAGTGA
0.86
3484

GCACA (1659)

stu-miR156a
TGACAGAAGAGAGTGA
0.86
3485

GCAC (1660)

stu-miR156b
TGACAGAAGAGAGAGA
0.9
3486

GCAC (1661)

stu-miR156c
TGACAGAAGAGAGAGA
0.9
3487

GCAC (1662)

stu-miR156d
TGACAGAAGAGAGAGA
0.9
3488

GCAC (1663)

stu-miR156e
TGACAGAAGAGAGAGA
0.9
3489

GCAC (1664)

tae-miR156
TGACAGAAGAGAGTGA
0.86
3490

GCACA (1665)

tae-miR156a
TGACAGAAGAGAGAGA
0.9
3491

GCAC (1666)

tae-miR156b
TGACAGAAGAGAGAGA
0.9
3492

GCAC (1667)

tcc-miR156a
TGACAGAAGAGAGAGA
0.9
3493

GCACA (1668)

tcc-miR156b
TGACAGAAGAGAGTGA
0.86
3494

GCAC (1669)

tcc-miR156c
TGACAGAAGAGAGTGA
0.86
3495

GCAC (1670)

tcc-miR156d
TGACAGAAGAGAGTGA
0.86
3496

GCAC (1671)

tcc-miR156e
TTGACAGAAGATAGAG
1
3497

AGCAC (1672)

tcc-miR156f
TTGACAGAAGATAGAG
1
3498

AGCAC (1673)

tcc-miR156g
TGACAGAAGAGAGTGA
0.86
3499

GCAC (1674)

tre-miR156
TGACAGAAGAGAGTGA
0.86
3500

GCAC (1675)

vvi-miR156a
TGACAGAAGAGAGGGA
0.86
3501

GCAC (1676)

vvi-miR156b
TGACAGAAGAGAGTGA
0.86
3502

GCAC (1677)

vvi-miR156c
TGACAGAAGAGAGTGA
0.86
3503

GCAC (1678)

vvi-miR156d
TGACAGAAGAGAGTGA
0.86
3504

GCAC (1679)

vvi-miR156e
TGACAGAGGAGAGTGA
0.81
3505

GCAC (1680)

vvi-miR156f
TTGACAGAAGATAGAG
1
3506

AGCAC (1681)

vvi-miR156g
TTGACAGAAGATAGAG
1
3507

AGCAC (1682)

vvi-miR156h
TGACAGAAGAGAGAGA
0.86
3508

GCAT (1683)

vvi-miR156i
TTGACAGAAGATAGAG
1
3509

AGCAC (1684)

zel-miR156
TGACAGAAGAGAGAGA
0.9
3510

GCAC (1685)

zma-miR156a
TGACAGAAGAGAGTGA
0.86
3511

GCAC (1686)

zma-miR156b
TGACAGAAGAGAGTGA
0.86
3512

GCAC (1687)

zma-miR156c
TGACAGAAGAGAGTGA
0.86
3513

GCAC (1688)

zma-miR156d
TGACAGAAGAGAGTGA
0.86
3514

GCAC (1689)

zma-miR156e
TGACAGAAGAGAGTGA
0.86
3515

GCAC (1690)

zma-miR156f
TGACAGAAGAGAGTGA
0.86
3516

GCAC (1691)

zma-miR156g
TGACAGAAGAGAGTGA
0.86
3517

GCAC (1692)

zma-miR156h
TGACAGAAGAGAGTGA
0.86
3518

GCAC (1693)

zma-miR156i
TGACAGAAGAGAGTGA
0.86
3519

GCAC (1694)

zma-miR156j
TGACAGAAGAGAGAGA
0.9
3520

GCACA (1695)

zma-miR156k
TGACAGAAGAGAGCGA
0.86
3521

GCAC (1696)

zma-miR1561
TGACAGAAGAGAGTGA
0.86
3522

GCAC (1697)

zma-miR156m
TGACAGAAGAGAGTGA
0.86
3523

GCAC (1698)

zma-miR156n
TGACAGAAGAGAGTGA
0.86
3524

GCAC (1699)

zma-miR156o
TGACAGAAGAGAGTGA
0.86
3525

GCAC (1700)

zma-miR156p
TGACAGAAGAGAGAGA
0.9
3526

GCAC (1701)

zma-miR156q
TGACAGAAGAGAGAGA
0.9
3527

GCAC (1702)

zma-miR156r
TGACAGAAGAGAGTGG
0.81
3528

GCAC (1703)

smo-
ahy-miR156a
TGACAGAAGAGAGAGA
0.9
3529

miR156b

GCAC (1704)

ahy-miR156b-5p
TTGACAGAAGATAGAG
0.95
3530

AGCAC (1705)

ahy-miR156c
TTGACAGAAGAGAGAG
0.9
3531

AGCAC (1706)

aly-miR156a
TGACAGAAGAGAGTGA
0.86
3532

GCAC (1707)

aly-miR156b
TGACAGAAGAGAGTGA
0.86
3533

GCAC (1708)

aly-miR156c
TGACAGAAGAGAGTGA
0.86
3534

GCAC (1709)

aly-miR156d
TGACAGAAGAGAGTGA
0.86
3535

GCAC (1710)

aly-miR156e
TGACAGAAGAGAGTGA
0.86
3536

GCAC (1711)

aly-miR156f
TGACAGAAGAGAGTGA
0.86
3537

GCAC (1712)

aly-miR156g
CGACAGAAGAGAGTGA
0.81
3538

GCAC (1713)

aly-miR156h
TGACAGAAGAAAGAGA
0.9
3539

GCAC (1714)

aqc-miR156a
TGACAGAAGATAGAGA
0.95
3540

GCAC (1715)

aqc-miR156b
TGACAGAAGATAGAGA
0.95
3541

GCAC (1716)

ath-miR156a
TGACAGAAGAGAGTGA
0.86
3542

GCAC (1717)

ath-miR156b
TGACAGAAGAGAGTGA
0.86
3543

GCAC (1718)

ath-miR156c
TGACAGAAGAGAGTGA
0.86
3544

GCAC (1719)

ath-miR156d
TGACAGAAGAGAGTGA
0.86
3545

GCAC (1720)

ath-miR156e
TGACAGAAGAGAGTGA
0.86
3546

GCAC (1721)

ath-miR156f
TGACAGAAGAGAGTGA
0.86
3547

GCAC (1722)

ath-miR156g
CGACAGAAGAGAGTGA
0.81
3548

GCAC (1723)

ath-miR156h
TGACAGAAGAAAGAGA
0.9
3549

GCAC (1724)

ath-miR156m
TGACAGAAGAGAGAGA
0.9
3550

GCAC (1725)

ath-miR156o
TGACAGAAGAGAGAGA
0.9
3551

GCAC (1726)

ath-miR156p
TGACAGAAGAGAGAGA
0.9
3552

GCAC (1727)

ath-miR156q
TGACAGAAGAGAGAGA
0.9
3553

GCAC (1728)

ath-miR156r
TGACAGAAGAGAGAGA
0.9
3554

GCAC (1729)

ath-miR156s
TGACAGAAGAGAGAGA
0.9
3555

GCAC (1730)

bdi-miR156
TGACAGAAGAGAGAGA
0.9
3556

GCACA (1731)

bdi-miR156b
TGACAGAAGAGAGTGA
0.86
3557

GCAC (1732)

bdi-miR156c
TGACAGAAGAGAGTGA
0.86
3558

GCAC (1733)

bdi-miR156d
TGACAGAAGAGAGTGA
0.86
3559

GCAC (1734)

bna-miR156a
TGACAGAAGAGAGTGA
0.86
3560

GCACA (1735)

bna-miR156b
TTGACAGAAGATAGAG
0.95
3561

AGCAC (1736)

bna-miR156c
TTGACAGAAGATAGAG
0.95
3562

AGCAC (1737)

can-miR156a
TGACAGAAGAGAGAGA
0.9
3563

GCAC (1738)

can-miR156b
TGACAGAAGAGAGGGA
0.86
3564

GCAC (1739)

cpt-miR156a
TGACAGAAGAGAGTGA
0.86
3565

GCAC (1740)

cpt-miR156b
TGACAGAAGAGAGAGA
0.9
3566

GCAC (1741)

cru-miR156
TGACAGAAGAGAGAGA
0.9
3567

GCAC (1742)

csi-miR156
TGACAGAAGAGAGTGA
0.86
3568

GCAC (1743)

csi-miR156a
TGACAGAAGAGAGAGA
0.9
3569

GCAC (1744)

csi-miR156b
TGACAGAAGAGAGAGA
0.9
3570

GCAC (1745)

ctr-miR156
TGACAGAAGAGAGTGA
0.86
3571

GCAC (1746)

eca-miR156
TGACAGAAGAGAGAGA
0.9
3572

GCAC (1747)

far-miR156a
TGACAGAAGAGAGAGA
0.9
3573

GCACA (1748)

far-miR156b
TTGACAGAAGAGAGAG
0.9
3574

AGCAC (1749)

ghr-miR156a
TGACAGAAGAGAGTGA
0.86
3575

GCAC (1750)

ghr-miR156b
TGACAGAAGAGAGTGA
0.86
3576

GCAC (1751)

ghr-miR156c
TGTCAGAAGAGAGTGA
0.81
3577

GCAC (1752)

ghr-miR156d
TGACAGAAGAGAGTGA
0.86
3578

GCAC (1753)

gma-miR156a
TGACAGAAGAGAGTGA
0.86
3579

GCAC (1754)

gma-miR156b
TGACAGAAGAGAGAGA
0.9
3580

GCACA (1755)

gma-miR156c
TTGACAGAAGATAGAG
0.95
3581

AGCAC (1756)

gma-miR156d
TTGACAGAAGATAGAG
0.95
3582

AGCAC (1757)

gma-miR156e
TTGACAGAAGATAGAG
0.95
3583

AGCAC (1758)

gma-miR156f
TTGACAGAAGAGAGAG
0.9
3584

AGCACA (1759)

gma-miR156g
ACAGAAGATAGAGAGC
0.86
3585

ACAG (1760)

gma-miR156h
TGACAGAAGAGAGAGA
0.9
3586

GCAC (1761)

gma-miR156i
TGACAGAAGAGAGAGA
0.9
3587

GCAC (1762)

han-miR156
TGACAGAAGAGAGAGA
0.9
3588

GCAC (1763)

hvs-miR156
TGACAGAAGAGAGAGA
0.9
3589

GCAC (1764)

hvu-miR156
TGACAGAAGAGAGTGA
0.86
3590

GCACA (1765)

hvv-miR156a
TGACAGAAGAGAGTGA
0.86
3591

GCAC (1766)

hvv-miR156b
TGACAGAAGAGAGAGA
0.9
3592

GCAC (1767)

hvv-miR156c
TGACAGAAGAGAGAGA
0.9
3593

GCAC (1768)

hvv-miR156d
TGACAGAAGAGAGAGA
0.9
3594

GCAC (1769)

lja-miR156
TGACAGAAGAGAGAGA
0.9
3595

GCAC (1770)

lsa-miR156
TGACAGAAGAGAGAGA
0.9
3596

GCAC (1771)

mdo-miR156a
TGACAGAAGAGAGAGA
0.9
3597

GCAC (1772)

mdo-miR156b
TGACAGAAGAGAGAGA
0.9
3598

GCAC (1773)

mtr-miR156
TGACAGAAGAGAGAGA
0.9
3599

GCACA (1774)

mtr-miR156b
TGACAGAAGAGAGTGA
0.86
3600

GCAC (1775)

mtr-miR156c
TGACAGAAGAGAGTGA
0.86
3601

GCAC (1776)

mtr-miR156d
TGACAGAAGAGAGTGA
0.86
3602

GCAC (1777)

mtr-miR156e
TTGACAGAAGATAGAG
0.95
3603

AGCAC (1778)

mtr-miR156f
TTGACAGAAGATAGAG
0.95
3604

AGCAC (1779)

mtr-miR156g
TTGACAGAAGATAGAG
0.9
3605

GGCAC (1780)

mtr-miR156h
TTGACAGAAGATAGAG
0.95
3606

AGCAC (1781)

mtr-miR156i
TGACAGAAGAGAGTGA
0.86
3607

GCAC (1782)

nbe-miR156a
TGACAGAAGAGAGAGA
0.9
3608

GCAC (1783)

nbe-miR156b
TGACAGAAGAGAGAGA
0.9
3609

GCAC (1784)

oru-miR156
TGACAGAAGAGAGTGA
0.86
3610

GCAC (1785)

osa-miR156a
TGACAGAAGAGAGTGA
0.86
3611

GCAC (1786)

osa-miR156b
TGACAGAAGAGAGTGA
0.86
3612

GCAC (1787)

osa-miR156c
TGACAGAAGAGAGTGA
0.86
3613

GCAC (1788)

osa-miR156d
TGACAGAAGAGAGTGA
0.86
3614

GCAC (1789)

osa-miR156e
TGACAGAAGAGAGTGA
0.86
3615

GCAC (1790)

osa-miR156f
TGACAGAAGAGAGTGA
0.86
3616

GCAC (1791)

osa-miR156g
TGACAGAAGAGAGTGA
0.86
3617

GCAC (1792)

osa-miR156h
TGACAGAAGAGAGTGA
0.86
3618

GCAC (1793)

osa-miR156i
TGACAGAAGAGAGTGA
0.86
3619

GCAC (1794)

osa-miR156j
TGACAGAAGAGAGTGA
0.86
3620

GCAC (1795)

osa-miR156k
TGACAGAAGAGAGAGA
0.9
3621

GCACA (1796)

osa-miR156l
CGACAGAAGAGAGTGA
0.76
3622

GCATA (1797)

osa-miR156m
TGACAGAAGAGAGTGA
0.86
3623

GCAC (1798)

osa-miR156n
TGACAGAAGAGAGTGA
0.86
3624

GCAC (1799)

osa-miR156o
TGACAGAAGAGAGTGA
0.81
3625

GCAT (1800)

osa-miR156p
TGACAGAAGAGAGTGA
0.81
3626

GCTC (1801)

osa-miR156q
TGACAGAACAGAGTGA
0.81
3627

GCAC (1802)

osa-miR156r
TGACAGAAGAGAGAGA
0.9
3628

GCAC (1803)

par-miR156
TGACAGAAGAGAGAGA
0.9
3629

GCAC (1804)

ppd-miR156
TGACAGAAGAGAGAGA
0.9
3630

GCAC (1805)

ppr-miR156
TGACAGAAGAGAGTGA
0.86
3631

GCAC (1806)

ppt-miR156a
TGACAGAAGAGAGTGA
0.86
3632

GCAC (1807)

ppt-miR156b
TGACAGAAGAGAGTGA
0.86
3633

GCAC (1808)

ppt-miR156c
TGACAGAAGAGAGTGA
0.86
3634

GCAC (1809)

pta-miR156a
CAGAAGATAGAGAGCA
0.81
3635

CATC (1810)

pta-miR156b
CAGAAGATAGAGAGCA
0.81
3636

CAAC (1811)

ptc-miR156a
TGACAGAAGAGAGTGA
0.86
3637

GCAC (1812)

ptc-miR156b
TGACAGAAGAGAGTGA
0.86
3638

GCAC (1813)

ptc-miR156c
TGACAGAAGAGAGTGA
0.86
3639

GCAC (1814)

ptc-miR156d
TGACAGAAGAGAGTGA
0.86
3640

GCAC (1815)

ptc-miR156e
TGACAGAAGAGAGTGA
0.86
3641

GCAC (1816)

ptc-miR156f
TGACAGAAGAGAGTGA
0.86
3642

GCAC (1817)

ptc-miR156g
TTGACAGAAGATAGAG
0.95
3643

AGCAC (1818)

ptc-miR156h
TTGACAGAAGATAGAG
0.95
3644

AGCAC (1819)

ptc-miR156i
TTGACAGAAGATAGAG
0.95
3645

AGCAC (1820)

ptc-miR156j
TTGACAGAAGATAGAG
0.95
3646

AGCAC (1821)

ptc-miR156k
TGACAGAAGAGAGGGA
0.86
3647

GCAC (1822)

ptr-miR156
TGACAGAAGAGAGAGA
0.9
3648

GCAC (1823)

pts-miR156a
TGACAGAAGAGAGTGA
0.81
3649

GCGC (1824)

pts-miR156b
TGACAGAAGAGAGAGA
0.9
3650

GCAC (1825)

pts-miR156c
TGACAGAAGAGAGAGA
0.9
3651

GCAC (1826)

rco-miR156a
TGACAGAAGAGAGTGA
0.86
3652

GCACA (1827)

rco-miR156b
TGACAGAAGAGAGTGA
0.86
3653

GCACA (1828)

rco-miR156c
TGACAGAAGAGAGTGA
0.86
3654

GCACA (1829)

rco-miR156d
TGACAGAAGAGAGTGA
0.86
3655

GCACA (1830)

rco-miR156e
TGACAGAAGAGAGAGA
0.9
3656

GCACA (1831)

rco-miR156f
TTGACAGAAGATAGAG
0.95
3657

AGCAC (1832)

rco-miR156g
TTGACAGAAGATAGAG
0.95
3658

AGCAC (1833)

rco-miR156h
TTGACAGAAGATAGAG
0.95
3659

AGCAC (1834)

sbi-miR156a
TGACAGAAGAGAGTGA
0.86
3660

GCAC (1835)

sbi-miR156b
TGACAGAAGAGAGTGA
0.86
3661

GCAC (1836)

sbi-miR156c
TGACAGAAGAGAGTGA
0.86
3662

GCAC (1837)

sbi-miR156d
TGACAGAAGAGAGAGA
0.9
3663

GCACA (1838)

sbi-miR156e
TGACAGAAGAGAGCGA
0.86
3664

GCAC (1839)

sbi-miR156f
TGACAGAAGAGAGTGA
0.86
3665

GCAC (1840)

sbi-miR156g
TGACAGAAGAGAGTGA
0.86
3666

GCAC (1841)

sbi-miR156h
TGACAGAAGAGAGTGA
0.86
3667

GCAC (1842)

sbi-miR156i
TGACAGAAGAGAGTGA
0.86
3668

GCAC (1843)

sin-miR156
TGACAGAAGAGAGAGA
0.9
3669

GCAC (1844)

sly-miR156a
TTGACAGAAGATAGAG
0.95
3670

AGCAC (1845)

sly-miR156b
TTGACAGAAGATAGAG
0.95
3671

AGCAC (1846)

sly-miR156c
TTGACAGAAGATAGAG
0.95
3672

AGCAC (1847)

smo-miR156a
CGACAGAAGAGAGTGA
0.81
3673

GCAC (1848)

smo-miR156c
TTGACAGAAGAAAGAG
0.9
3674

AGCAC (1849)

smo-miR156d
TTGACAGAAGACAGGG
0.86
3675

AGCAC (1850)

sof-miR156
TGACAGAAGAGAGTGA
0.86
3676

GCAC (1851)

sof-miR156c
TGACAGAAGAGAGAGA
0.9
3677

GCAC (1852)

sof-miR156d
TGACAGAAGAGAGAGA
0.9
3678

GCAC (1853)

sof-miR156e
TGACAGAAGAGAGAGA
0.9
3679

GCAC (1854)

sof-miR156f
TGACAGAAGAGAGAGA
0.9
3680

GCAC (1855)

sof-miR156g
TGACAGAAGAGAGAGA
0.9
3681

GCAC (1856)

sof-miR156h
TGACAGAAGAGAGAGA
0.9
3682

GCAC (1857)

sof-miR156u
TGACAGAAGAGAGAGA
0.9
3683

GCAC (1858)

spr-miR156
TGACAGAAGAGAGAGA
0.9
3684

GCAC (1859)

ssp-miR156
TGACAGAAGAGAGTGA
0.86
3685

GCACA (1860)

stu-miR156a
TGACAGAAGAGAGTGA
0.86
3686

GCAC (1861)

stu-miR156b
TGACAGAAGAGAGAGA
0.9
3687

GCAC (1862)

stu-miR156c
TGACAGAAGAGAGAGA
0.9
3688

GCAC (1863)

stu-miR156d
TGACAGAAGAGAGAGA
0.9
3689

GCAC (1864)

stu-miR156e
TGACAGAAGAGAGAGA
0.9
3690

GCAC (1865)

tae-miR156
TGACAGAAGAGAGTGA
0.86
3691

GCACA (1866)

tae-miR156a
TGACAGAAGAGAGAGA
0.9
3692

GCAC (1867)

tae-miR156b
TGACAGAAGAGAGAGA
0.9
3693

GCAC (1868)

tcc-miR156a
TGACAGAAGAGAGAGA
0.9
3694

GCACA (1869)

tcc-miR156b
TGACAGAAGAGAGTGA
0.86
3695

GCAC (1870)

tcc-miR156c
TGACAGAAGAGAGTGA
0.86
3696

GCAC (1871)

tcc-miR156d
TGACAGAAGAGAGTGA
0.86
3697

GCAC (1872)

tcc-miR156e
TTGACAGAAGATAGAG
0.95
3698

AGCAC (1873)

tcc-miR156f
TTGACAGAAGATAGAG
0.95
3699

AGCAC (1874)

tcc-miR156g
TGACAGAAGAGAGTGA
0.86
3700

GCAC (1875)

tre-miR156
TGACAGAAGAGAGTGA
0.86
3701

GCAC (1876)

vvi-miR156a
TGACAGAAGAGAGGGA
0.86
3702

GCAC (1877)

vvi-miR156b
TGACAGAAGAGAGTGA
0.86
3703

GCAC (1878)

vvi-miR156c
TGACAGAAGAGAGTGA
0.86
3704

GCAC (1879)

vvi-miR156d
TGACAGAAGAGAGTGA
0.86
3705

GCAC (1880)

vvi-miR156e
TGACAGAGGAGAGTGA
0.81
3706

GCAC (1881)

vvi-miR156f
TTGACAGAAGATAGAG
0.95
3707

AGCAC (1882)

vvi-miR156g
TTGACAGAAGATAGAG
0.95
3708

AGCAC (1883)

vvi-miR156h
TGACAGAAGAGAGAGA
0.86
3709

GCAT (1884)

vvi-miR156i
TTGACAGAAGATAGAG
0.95
3710

AGCAC (1885)

zel-miR156
TGACAGAAGAGAGAGA
0.9
3711

GCAC (1886)

zma-miR156a
TGACAGAAGAGAGTGA
0.86
3712

GCAC (1887)

zma-miR156b
TGACAGAAGAGAGTGA
0.86
3713

GCAC (1888)

zma-miR156c
TGACAGAAGAGAGTGA
0.86
3714

GCAC (1889)

zma-miR156d
TGACAGAAGAGAGTGA
0.86
3715

GCAC (1890)

zma-miR156e
TGACAGAAGAGAGTGA
0.86
3716

GCAC (1891)

zma-miR156f
TGACAGAAGAGAGTGA
0.86
3717

GCAC (1892)

zma-miR156g
TGACAGAAGAGAGTGA
0.86
3718

GCAC (1893)

zma-miR156h
TGACAGAAGAGAGTGA
0.86
3719

GCAC (1894)

zma-miR156i
TGACAGAAGAGAGTGA
0.86
3720

GCAC (1895)

zma-miR156j
TGACAGAAGAGAGAGA
0.9
3721

GCACA (1896)

zma-miR156k
TGACAGAAGAGAGCGA
0.86
3722

GCAC (1897)

zma-miR156l
TGACAGAAGAGAGTGA
0.86
3723

GCAC (1898)

zma-miR156m
TGACAGAAGAGAGTGA
0.86
3724

GCAC (1899)

zma-miR156n
TGACAGAAGAGAGTGA
0.86
3725

GCAC (1900)

zma-miR156o
TGACAGAAGAGAGTGA
0.86
3726

GCAC (1901)

zma-miR156p
TGACAGAAGAGAGAGA
0.9
3727

GCAC (1902)

zma-miR156q
TGACAGAAGAGAGAGA
0.9
3728

GCAC (1903)

zma-miR156r
TGACAGAAGAGAGTGG
0.81
3729

GCAC (1904)

gma-
acb-miR159
TTGGACTGAAGGGAGCT
0.81
3730

miR159a-

CCCT (1905)

3p

aha-miR159
TTGGACTGAAGGGAGCT
0.81
3731

CCCT (1906)

ahi-miR159
TTGGACTGAAGGGAGCT
0.81
3732

CCCT (1907)

ahy-miR159
TTTGGATTGAAGGGAGC
1
3733

TCTA (1908)

aly-miR159a
TTTGGATTGAAGGGAGC
1
3734

TCTA (1909)

aly-miR159b
TTTGGATTGAAGGGAGC
0.95
3735

TCTT (1910)

aly-miR159c
TTTGGATTGAAGGGAGC
0.9
3736

TCCT (1911)

ape-miR159
TTGGACTGAAGGGAGCT
0.81
3737

CCCT (1912)

aqc-miR159
TTTGGACTGAAGGGAGC
0.95
3738

TCTA (1913)

ath-miR159a
TTTGGATTGAAGGGAGC
1
3739

TCTA (1914)

ath-miR159b
TTTGGATTGAAGGGAGC
0.95
3740

TCTT (1915)

ath-miR159c
TTTGGATTGAAGGGAGC
0.9
3741

TCCT (1916)

bdi-miR159
CTTGGATTGAAGGGAGC
0.9
3742

TCT (1917)

bna-miR159
TTTGGATTGAAGGGAGC
1
3743

TCTA (1918)

bra-miR159a
TTTGGATTGAAGGGAGC
1
3744

TCTA (1919)

bvl-miR159
TTGGACTGAAGGGAGCT
0.81
3745

CCCT (1920)

cmi-miR159
TTGGACTGAAGGGAGCT
0.81
3746

CCCT (1921)

cor-miR159
TTGGACTGAAGGGAGCT
0.81
3747

CCCT (1922)

crb-miR159
TTGGACTGAAGGGAGCT
0.81
3748

CCCT (1923)

csi-miR159
TTTGGATTGAAGGGAGC
1
3749

TCTA (1924)

dso-miR159
TTGGACTGAAGGGAGCT
0.81
3750

CCCT (1925)

ech-miR159
TTGGACTGAAGGGAGCT
0.81
3751

CCCT (1926)

fal-miR159
TTGGACTGAAGGGAGCT
0.81
3752

CCCT (1927)

far-miR159
TTTGGATTGAAGGGAGC
0.95
3753

TCTG (1928)

gma-miR159b
ATTGGAGTGAAGGGAG
0.86
3754

CTCCA (1929)

gma-miR159c
ATTGGAGTGAAGGGAG
0.81
3755

CTCCG (1930)

hvu-miR159a
TTTGGATTGAAGGGAGC
0.95
3756

TCTG (1931)

hvu-miR159b
TTTGGATTGAAGGGAGC
0.95
3757

TCTG (1932)

hvv-miR159a
TTTGGATTGAAGGGAGC
0.95
3758

TCTG (1933)

hvv-miR159b
TTTGGATTGAAGGGAGC
0.95
3759

TCTG (1934)

ltu-miR159
TTTGGATTGAAGGGAGC
1
3760

TCTA (1935)

mma-miR159
TTGGACTGAAGGGAGCT
0.81
3761

CCCT (1936)

mtr-miR159a
TTTGGATTGAAGGGAGC
1
3762

TCTA (1937)

mtr-miR159b
ATTGAATTGAAGGGAG
0.71
3763

CAACT (1938)

mtr-miR159c
TTTGGATTGAAGGGAGC
1
3764

TCTA (1939)

nof-miR159
TTGGACTGAAGGGAGCT
0.81
3765

CCCT (1940)

oru-miR159
TTTGGATTGAAGGGAGC
0.95
3766

TCTG (1941)

osa-miR159a
TTTGGATTGAAGGGAGC
0.95
3767

TCTG (1942)

osa-miR159a.1
TTTGGATTGAAGGGAGC
0.95
3768

TCTG (1943)

osa-miR159b
TTTGGATTGAAGGGAGC
0.95
3769

TCTG (1944)

osa-miR159c
ATTGGATTGAAGGGAG
0.9
3770

CTCCA (1945)

osa-miR159d
ATTGGATTGAAGGGAG
0.86
3771

CTCCG (1946)

osa-miR159e
ATTGGATTGAAGGGAG
0.86
3772

CTCCT (1947)

osa-miR159f
CTTGGATTGAAGGGAGC
0.95
3773

TCTA (1948)

osa-miR159m
TTTGGATTGAAGGGAGC
0.95
3774

TCTG (1949)

pgl-miR159
TTTGGATTGAAGGGAGC
0.95
3775

TCTG (1950)

psi-miR159
CTTGGATTGAAGGGAGC
0.9
3776

TCCA (1951)

pta-miR159a
TTGGATTGAAGGGAGCT
0.9
3777

CCA (1952)

pta-miR159b
TTGGATTGAAGAGAGCT
0.81
3778

CCC (1953)

pta-miR159c
CTTGGATTGAAGGGAGC
0.86
3779

TCCC (1954)

ptc-miR159a
TTTGGATTGAAGGGAGC
1
3780

TCTA (1955)

ptc-miR159b
TTTGGATTGAAGGGAGC
1
3781

TCTA (1956)

ptc-miR159c
TTTGGATTGAAGGGAGC
1
3782

TCTA (1957)

ptc-miR159d
CTTGGATTGAAGGGAGC
0.86
3783

TCCT (1958)

ptc-miR159e
CTTGGGGTGAAGGGAG
0.76
3784

CTCCT (1959)

ptc-miR159f
ATTGGAGTGAAGGGAG
0.86
3785

CTCGA (1960)

pvu-miR159
TTTGGATTGAAGGGAGC
1
3786

TCTA (1961)

pvu-miR159a.1
TTTGGATTGAAGGGAGC
1
3787

TCTA (1962)

rco-miR159
TTTGGATTGAAGGGAGC
1
3788

TCTA (1963)

rin-miR159
TTGGACTGAAGGGAGCT
0.81
3789

CCCT (1964)

sar-miR159
TTTGGATTGAAGGGAGC
0.95
3790

TCTG (1965)

sbi-miR159a
TTTGGATTGAAGGGAGC
0.95
3791

TCTG (1966)

sbi-miR159b
CTTGGATTGAAGGGAGC
0.86
3792

TCCT (1967)

sly-miR159
TTTGGATTGAAGGGAGC
1
3793

TCTA (1968)

smo-miR159
CTTGGATTGAAGGGAGC
0.86
3794

TCCC (1969)

sof-miR159a
TTTGGATTGAAGGGAGC
0.95
3795

TCTG (1970)

sof-miR159b
TTTGGATTGAAGGGAGC
0.95
3796

TCTG (1971)

sof-miR159c
CTTGGATTGAAGGGAGC
0.86
3797

TCCT (1972)

sof-miR159d
TTTGGATTGAAGGGAGC
0.95
3798

TCTG (1973)

sof-miR159e
TTTGGATTGAAAGGAGC
0.9
3799

TCTT (1974)

spr-miR159
TTTGGATTGAAGGGAGC
0.95
3800

TCTG (1975

ssp-miR159a
TTTGGATTGAAG)GGAGC
0.95
3801

TCTG (1976

svi-miR159
TTGGACTGAAG)GGAGCT
0.81
3802

CCCT (1977

tae-miR159a
TTTGGATTGAAGGGAGC
0.95
3803

TCTG (1978)

tae-miR159b
TTTGGATTGAAGGGAGC
0.95
3804

TCTG (1979)

tar-miR159
TTGGACTGAAGGGAGCT
0.81
3805

CCCT (1980)

vvi-miR159a
CTTGGAGTGAAGGGAG
0.86
3806

CTCTC (1981)

vvi-miR159b
CTTGGAGTGAAGGGAG
0.86
3807

CTCTC (1982)

vvi-miR159c
TTTGGATTGAAGGGAGC
1
3808

TCTA (1983)

zma-miR159a
TTTGGATTGAAGGGAGC
0.95
3809

TCTG (1984)

zma-miR159b
TTTGGATTGAAGGGAGC
0.95
3810

TCTG (1985)

zma-miR159c
CTTGGATTGAAGGGAGC
0.86
3811

TCCT (1986)

zma-miR159d
CTTGGATTGAAGGGAGC
0.86
3812

TCCT (1987)

zma-miR159e
ATTGGTTTGAAGGGAGC
0.86
3813

TCCA (1988)

zma-miR159f
TTTGGATTGAAGGGAGC
0.95
3814

TCTG (1989)

zma-miR159g
TTTGGAGTGAAGGGAGT
0.86
3815

TCTG (1990)

zma-miR159h
TTTGGAGTGAAGGGAG
0.9
3816

CTCTG (1991)

zma-miR159i
TTTGGAGTGAAGGGAG
0.9
3817

CTCTG (1992)

zma-miR159j
TTTGGATTGAAGGGAGC
0.95
3818

TCTG (1993)

zma-miR159k
TTTGGATTGAAGGGAGC
0.95
3819

TCTG (1994)

zma-miR159m
TTTGGATTGAAGGGAGC
0.95
3820

TCTG (1995)

sbi-
acb-miR159
TTGGACTGAAGGGAGCT
0.81
3821

miR159a

CCCT (1996)

aha-miR159
TTGGACTGAAGGGAGCT
0.81
3822

CCCT (1997)

ahi-miR159
TTGGACTGAAGGGAGCT
0.81
3823

CCCT (1998)

ahy-miR159
TTTGGATTGAAGGGAGC
0.95
3824

TCTA (1999)

aly-miR159a
TTTGGATTGAAGGGAGC
0.95
3825

TCTA (2000)

aly-miR159b
TTTGGATTGAAGGGAGC
0.95
3826

TCTT (2001)

aly-miR159c
TTTGGATTGAAGGGAGC
0.9
3827

TCCT (2002)

ape-miR159
TTGGACTGAAGGGAGCT
0.81
3828

CCCT (2003)

aqc-miR159
TTTGGACTGAAGGGAGC
0.9
3829

TCTA (2004)

ath-miR159a
TTTGGATTGAAGGGAGC
0.95
3830

TCTA (2005)

ath-miR159b
TTTGGATTGAAGGGAGC
0.95
3831

TCTT (2006)

ath-miR159c
TTTGGATTGAAGGGAGC
0.9
3832

TCCT (2007)

bdi-miR159
CTTGGATTGAAGGGAGC
0.9
3833

TCT (2008)

bna-miR159
TTTGGATTGAAGGGAGC
0.95
3834

TCTA (2009)

bra-miR159a
TTTGGATTGAAGGGAGC
0.95
3835

TCTA (2010)

bvl-miR159
TTGGACTGAAGGGAGCT
0.81
3836

CCCT (2011)

cmi-miR159
TTGGACTGAAGGGAGCT
0.81
3837

CCCT (2012)

cor-miR159
TTGGACTGAAGGGAGCT
0.81
3838

CCCT (2013)

crb-miR159
TTGGACTGAAGGGAGCT
0.81
3839

CCCT (2014)

csi-miR159
TTTGGATTGAAGGGAGC
0.95
3840

TCTA (2015)

dso-miR159
TTGGACTGAAGGGAGCT
0.81
3841

CCCT (2016)

ech-miR159
TTGGACTGAAGGGAGCT
0.81
3842

CCCT (2017)

fal-miR159
TTGGACTGAAGGGAGCT
0.81
3843

CCCT (2018)

far-miR159
TTTGGATTGAAGGGAGC
1
3844

TCTG (2019)

gma-miR159a-3p
TTTGGATTGAAGGGAGC
0.95
3845

TCTA (2020)

gma-miR159b
ATTGGAGTGAAGGGAG
0.81
3846

CTCCA (2021)

gma-miR159c
ATTGGAGTGAAGGGAG
0.86
3847

CTCCG (2022)

hvu-miR159a
TTTGGATTGAAGGGAGC
1
3848

TCTG (2023)

hvu-miR159b
TTTGGATTGAAGGGAGC
1
3849

TCTG (2024)

hvv-miR159a
TTTGGATTGAAGGGAGC
1
3850

TCTG (2025)

hvv-miR159b
TTTGGATTGAAGGGAGC
1
3851

TCTG (2026)

ltu-miR159
TTTGGATTGAAGGGAGC
0.95
3852

TCTA (2027)

mma-miR159
TTGGACTGAAGGGAGCT
0.81
3853

CCCT (2028)

mtr-miR159a
TTTGGATTGAAGGGAGC
0.95
3854

TCTA (2029)

mtr-miR159b
ATTGAATTGAAGGGAG
0.71
3855

CAACT (2030)

mtr-miR159c
TTTGGATTGAAGGGAGC
0.95
3856

TCTA (2031)

nof-miR159
TTGGACTGAAGGGAGCT
0.81
3857

CCCT (2032)

oru-miR159
TTTGGATTGAAGGGAGC
1
3858

TCTG (2033)

osa-miR159a
TTTGGATTGAAGGGAGC
1
3859

TCTG (2034)

osa-miR159a.1
TTTGGATTGAAGGGAGC
1
3860

TCTG (2035)

osa-miR159b
TTTGGATTGAAGGGAGC
1
3861

TCTG (2036)

osa-miR159c
ATTGGATTGAAGGGAG
0.86
3862

CTCCA (2037)

osa-miR159d
ATTGGATTGAAGGGAG
0.9
3863

CTCCG (2038)

osa-miR159e
ATTGGATTGAAGGGAG
0.86
3864

CTCCT (2039)

osa-miR159f
CTTGGATTGAAGGGAGC
0.9
3865

TCTA (2040)

osa-miR159m
TTTGGATTGAAGGGAGC
1
3866

TCTG (2041)

pgl-miR159
TTTGGATTGAAGGGAGC
1
3867

TCTG (2042)

psi-miR159
CTTGGATTGAAGGGAGC
0.86
3868

TCCA (2043)

pta-miR159a
TTGGATTGAAGGGAGCT
0.86
3869

CCA (2044)

pta-miR159b
TTGGATTGAAGAGAGCT
0.81
3870

CCC (2045)

pta-miR159c
CTTGGATTGAAGGGAGC
0.86
3871

TCCC (2046)

ptc-miR159a
TTTGGATTGAAGGGAGC
0.95
3872

TCTA (2047)

ptc-miR159b
TTTGGATTGAAGGGAGC
0.95
3873

TCTA (2048)

ptc-miR159c
TTTGGATTGAAGGGAGC
0.95
3874

TCTA (2049)

ptc-miR159d
CTTGGATTGAAGGGAGC
0.86
3875

TCCT (2050)

ptc-miR159e
CTTGGGGTGAAGGGAG
0.76
3876

CTCCT (2051)

ptc-miR159f
ATTGGAGTGAAGGGAG
0.81
3877

CTCGA (2052)

pvu-miR159
TTTGGATTGAAGGGAGC
0.95
3878

TCTA (2053)

pvu-miR159a.1
TTTGGATTGAAGGGAGC
0.95
3879

TCTA (2054)

rco-miR159
TTTGGATTGAAGGGAGC
0.95
3880

TCTA (2055)

rin-miR159
TTGGACTGAAGGGAGCT
0.81
3881

CCCT (2056)

sar-miR159
TTTGGATTGAAGGGAGC
1
3882

TCTG (2057)

sbi-miR159b
CTTGGATTGAAGGGAGC
0.86
3883

TCCT (2058)

sly-miR159
TTTGGATTGAAGGGAGC
0.95
3884

TCTA (2059)

smo-miR159
CTTGGATTGAAGGGAGC
0.86
3885

TCCC (2060)

sof-miR159a
TTTGGATTGAAGGGAGC
1
3886

TCTG (2061)

sof-miR159b
TTTGGATTGAAGGGAGC
1
3887

TCTG (2062)

sof-miR159c
CTTGGATTGAAGGGAGC
0.86
3888

TCCT (2063)

sof-miR159d
TTTGGATTGAAGGGAGC
1
3889

TCTG (2064)

sof-miR159e
TTTGGATTGAAAGGAGC
0.9
3890

TCTT (2065)

spr-miR159
TTTGGATTGAAGGGAGC
1
3891

TCTG (2066)

ssp-miR159a
TTTGGATTGAAGGGAGC
1
3892

TCTG (2067)

svi-miR159
TTGGACTGAAGGGAGCT
0.81
3893

CCCT (2068)

tae-miR159a
TTTGGATTGAAGGGAGC
1
3894

TCTG (2069)

tae-miR159b
TTTGGATTGAAGGGAGC
1
3895

TCTG (2070)

tar-miR159
TTGGACTGAAGGGAGCT
0.81
3896

CCCT (2071)

vvi-miR159a
CTTGGAGTGAAGGGAG
0.86
3897

CTCTC (2072)

vvi-miR159b
CTTGGAGTGAAGGGAG
0.86
3898

CTCTC (2073)

vvi-miR159c
TTTGGATTGAAGGGAGC
0.95
3899

TCTA (2074)

zma-miR159a
TTTGGATTGAAGGGAGC
1
3900

TCTG (2075)

zma-miR159b
TTTGGATTGAAGGGAGC
1
3901

TCTG (2076)

zma-miR159c
CTTGGATTGAAGGGAGC
0.86
3902

TCCT (2077)

zma-miR159d
CTTGGATTGAAGGGAGC
0.86
3903

TCCT (2078)

zma-miR159e
ATTGGTTTGAAGGGAGC
0.81
3904

TCCA (2079)

zma-miR159f
TTTGGATTGAAGGGAGC
1
3905

TCTG (2080)

zma-miR159g
TTTGGAGTGAAGGGAGT
0.9
3906

TCTG (2081)

zma-miR159h
TTTGGAGTGAAGGGAG
0.95
3907

CTCTG (2082)

zma-miR159i
TTTGGAGTGAAGGGAG
0.95
3908

CTCTG (2083)

zma-miR159j
TTTGGATTGAAGGGAGC
1
3909

TCTG (2084)

zma-miR159k
TTTGGATTGAAGGGAGC
1
3910

TCTG (2085)

zma-miR159m
TTTGGATTGAAGGGAGC
1
3911

TCTG (2086)

TABLE 8

Summary of Homologs (Orthologs to Small RNAs which are down-

regulated in Abiotic Stress in Soybean Plants.

Homolog

%
Homolog Stem-loop

Mir Name
Name
Homolog Sequence (SEQ ID NO:)
Identity
Sequence

aly-miR396a-
aly-
GCTCAAGAAAGCTGTGGGAAA (3953)
0.86
5117

3p
miR396b-3p

csi-miR396c
TTCAAGAAATCTGTGGGAAG (3954)
0.86
5118

gma-
AAGAAAGCTGTGGGAGAATATGGC (3955)
0.67
5119

miR396d

osa-
GTTCAAGAAAGCCCATGGAAA (3956)
0.71
5120

miR396e*

osa-
ATGGTTCAAGAAAGCCCATGGAAA (3957)
0.71
5121

miR396e-3p

osa-
GTTCAAGAAAGTCCTTGGAAA (3958)
0.71
5122

miR396f*

osa-
ATAGTTCAAGAAAGTCCTTGGAAA
0.71
5123

miR396f-3p
(3959)

zma-
GTTCAATAAAGCTGTGGGAAA (3960)
0.95
5124

miR396a*

zma-
GTTCAATAAAGCTGTGGGAAA (3961)
0.95
5125

miR396b-3p

zma-
GGTCAAGAAAGCCGTGGGAAG (3962)
0.86
5126

miR396e*

zma-
GGTCAAGAAAGCTGTGGGAAG (3963)
0.9
5127

miR396f*

zma-
GTTCAAGAAAGCTGTGGAAGA (3964)
0.81
5128

miR396g*

aly-miR396b-
aly-
GTTCAATAAAGCTGTGGGAAG (3965)
0.86
5129

3p
miR396a-3p

csi-miR396c
TTCAAGAAATCTGTGGGAAG (3966)
0.81
5130

gma-
AAGAAAGCTGTGGGAGAATATGGC (3967)
0.76
5131

miR396d

osa-
GTTCAAGAAAGCCCATGGAAA (3968)
0.76
5132

miR396e*

osa-
ATGGTTCAAGAAAGCCCATGGAAA (3969)
0.76
5133

miR396e-3p

osa-
GTTCAAGAAAGTCCTTGGAAA (3970)
0.76
5134

miR396f*

osa-
ATAGTTCAAGAAAGTCCTTGGAAA
0.76
5135

miR396f-3p
(3971)

zma-
GTTCAATAAAGCTGTGGGAAA (3972)
0.9
5136

miR396a*

zma-
GTTCAATAAAGCTGTGGGAAA (3973)
0.9
5137

miR396b-3p

zma-
GGTCAAGAAAGCCGTGGGAAG (3974)
0.86
5138

miR396e*

zma-
GGTCAAGAAAGCTGTGGGAAG (3975)
0.9
5139

miR396f*

zma-
GTTCAAGAAAGCTGTGGAAGA (3976)
0.86
5140

miR396g*

ath-
aly-
CCCGCCTTGCATCAACTGAAT (3977)
0.95
5141

miRf10239-
miR168a*

akr

bna-
aly-
TAATCTGCATCCTGAGGTTTA (3978)
1
5142

miR2111b-5p
miR2111a

aly-
TAATCTGCATCCTGAGGTTTA (3979)
1
5143

miR2111b

ath-
TAATCTGCATCCTGAGGTTTA (3980)
1
5144

miR2111a

ath-
TAATCTGCATCCTGAGGTTTA (3981)
1
5145

miR2111b

bna-
TAATCTGCATCCTGAGGTTTA (3982)
1
5146

miR2111a

bra-
TAATCTGCATCCTGAGGTTTA (3983)
1
5147

miR2111a

bra-
TAATCTGCATCCTGAGGTTTA (3984)
1
5148

miR2111b

lja-miR2111
TAATCTGCATCCTGAGGTTTA (3985)
1
5149

mtr-
TAATCTGCATCCTGAGGTTTA (3986)
1
5150

miR2111a

mtr-
TAATCTGCATCCTGAGGTTTA (3987)
1
5151

miR2111b

mtr-
TAATCTGCATCCTGAGGTTTA (3988)
1
5152

miR2111c

mtr-
TAATCTGCATCCTGAGGTTTA (3989)
1
5153

miR2111d

mtr-
TAATCTGCATCCTGAGGTTTA (3990)
1
5154

miR2111e

mtr-
TAATCTGCATCCTGAGGTTTA (3991)
1
5155

miR2111f

mtr-
TAATCTGCATCCTGAGGTTTA (3992)
1
5156

miR2111h

mtr-
TAATCTGCATCCTGAGGTTTA (3993)
1
5157

miR2111i

mtr-
TAATCTGCATCCTGAGGTTTA (3994)
1
5158

miR2111j

mtr-
TAATCTGCATCCTGAGGTTTA (3995)
1
5159

miR2111k

mtr-
TAATCTGCATCCTGAGGTTTA (3996)
1
5160

miR2111l

mtr-
TAATCTGCATCCTGAGGTTTA (3997)
1
5161

miR2111m

mtr-
TAATCTGCATCCTGAGGTTTA (3998)
1
5162

miR2111n

mtr-
TAATCTGCATCCTGAGGTTTA (3999)
1
5163

miR2111o

mtr-
TAATCTGCATCCTGAGGTTTA (4000)
1
5164

miR2111p

mtr-
TAATCTGCATCCTGAGGTTTA (4001)
1
5165

miR2111q

mtr-
TAATCTGCATCCTGAGGTTTA (4002)
1
5166

miR2111r

mtr-
TAATCTGCATCCTGAGGTTTA (4003)
1
5167

miR2111s

tcc-miR2111
TAATCTGCATCCTGAGGTTTA (4004)
1
5168

vvi-
TAATCTGCATCCTGAGGTCTA (4005)
0.95
5169

miR2111-5p

csi-miR162-
aly-
GGAGGCAGCGGTTCATCGATC (4006)
0.95
5170

5p
miR162a*

aly-
GGAGGCAGCGGTTCATCGATC (4007)
0.95
5171

miR162b*

zma-
GGGCGCAGTGGTTTATCGATC (4008)
0.77
5172

miR162*

gma-miR396d
aly-
GCTCAAGAAAGCTGTGGGAAA (4009)
0.67
5173

miR396b-3p

zma-
GTTCAATAAAGCTGTGGGAAA (4010)
0.63
5174

miR396a*

zma-
GTTCAATAAAGCTGTGGGAAA (4011)
0.63
5175

miR396b-3p

zma-
GGTCAAGAAAGCTGTGGGAAG (4012)
0.63
5176

miR396f*

zma-
GTTCAAGAAAGCTGTGGAAGA (4013)
0.67
5177

miR396g*

gma-
aqc-miR482a
TCTTGCCGACTCCTCCCATACC (4014)
0.71
5178

miR482a-3p

aqc-
TCTTGCCGACTCCTCCCATACC (4015)
0.71
5179

miR482b

aqc-miR482c
TCTTGCCGACTCCTCCCATACC (4016)
0.71
5180

csi-miR482a
TCTTCCCTATGCCTCCCATTCC (4017)
0.79
5181

csi-miR482b
TCTTGCCCACCCCTCCCATTCC (4018)
0.71
5182

csi-miR482c
TTCCCTAGTCCCCCTATTCCTA (4019)
0.75
5183

ghr-miR482a
TCTTTCCTACTCCTCCCATACC (4020)
0.71
5184

ghr-miR482b
TCTTGCCTACTCCACCCATGCC (4021)
0.71
5185

gma-miR482
TCTTCCCAATTCCGCCCATTCCTA (4022)
1
5186

gra-miR482
TCTTTCCAATTCCTCCCATTCC (4023)
0.83
5187

gso-miR482a
TCTTCCCTACACCTCCCATAC (4024)
0.67
5188

gso-
TCTTCCCTACACCTCCCATAC (4025)
0.67
5189

miR482b

mdm-
TCTTCCCAAGCCCGCCCATTCC (4026)
0.83
5190

miR482

mdo-miR482
TCTTCCCAAGCCCGCCCATTCC (4027)
0.83
5191

pab-
TCTTCCCTACTCCTCCCATTCC (4028)
0.79
5192

miR482a

pab-
TCTTCCCTATTCCTCCCATTCC (4029)
0.83
5193

miR482b

pab-
TCTTTCCTACTCCTCCCATTCC (4030)
0.75
5194

miR482c

pta-miR482a
TCTTCCCTACTCCTCCCATTCC (4031)
0.79
5195

pta-miR482b
TCTTCCCTACTCCTCCCATTCC (4032)
0.79
5196

pta-miR482c
TCTTCCCTATTCCTCCCATT (4033)
0.75
5197

pta-miR482d
TCCTCCCTACTCCTCCCATT (4034)
0.67
5198

ptc-
TCTTGCCTACTCCTCCCATT (4035)
0.67
5199

miR482.2

pvu-miR482
TCTTCCCAATTCCGCCCATTCC (4036)
0.92
5200

sly-miR482
TTTCCAATTCCACCCATTCCTA (4037)
0.83
5201

vvi-miR482
TCTTTCCTACTCCTCCCATTCC (4038)
0.75
5202

zma-miR482
TCTTCCTTGTTCCTCCCATT (4039)
0.67
5203

gma-
pvu-
GGAATGGGCTGATTGGGAAGCA (4040)
0.73
5204

miR482b-5p
miR482*

gso-
aly-
GGCAAGTTGTCCTTGGCTACA (4041)
0.85
5205

miR169g*
miR169a*

aly-
GGCAAGTTGTCCTTCGGCTACA (4042)
0.85
5206

miR169b*

aly-
GGCAAGTCATCTCTGGCTATG (4043)
0.65
5207

miR169c*

aly-
GCAAGTTGACCTTGGCTCTGT (4044)
0.8
5208

miR169d*

aly-
GCAAGTTGACCTTGGCTCTGT (4045)
0.8
5209

miR169e*

aly-
GCAAGTTGACCTTGGCTCTGC (4046)
0.8
5210

miR169f*

aly-
GCAAGTTGACCTTGGCTCTGT (4047)
0.8
5211

miR169g*

aly-miR169h
TAGCCAAGGATGACTTGCCTG (4048)
0.65
5212

aly-
GGCAGTCTCCTTGGCTATT (4049)
0.65
5213

miR169h*

aly-miR169i
TAGCCAAGGATGACTTGCCTG (4050)
0.65
5214

aly-
GGCAGTCTCCTTGGATATC (4051)
0.6
5215

miR169i*

aly-miR169j
TAGCCAAGGATGACTTGCCTG (4052)
0.65
5216

aly-
GGCAGTCTCCTTGGCTATC (4053)
0.65
5217

miR169j*

aly-miR169k
TAGCCAAGGATGACTTGCCTG (4054)
0.65
5218

aly-
GGCAGTCTCCTTGGCTATC (4055)
0.65
5219

miR169k*

aly-miR169l
TAGCCAAGGATGACTTGCCTG (4056)
0.65
5220

aly-
GGCAGTCTCCTTGGCTATC (4057)
0.65
5221

miR169l*

aly-
TAGCCAAGGATGACTTGCCTG (4058)
0.65
5222

miR169m

aly-
GGCAGTCTTCTTGGCTATC (4059)
0.6
5223

miR169m*

aly-miR169n
TAGCCAAAGATGACTTGCCTG (4060)
0.6
5224

aly-
GGCAGTCTCTTTGGCTATC (4061)
0.6
5225

miR169n*

aqc-miR169a
TAGCCAAGGATGACTTGCCTA (4062)
0.65
5226

aqc-
TAGCCAAGGATGACTTGCCTG (4063)
0.65
5227

miR169b

ath-
TCCGGCAAGTTGACCTTGGCT (4064)
0.9
5228

miR169g*

ath-miR169h
TAGCCAAGGATGACTTGCCTG (4065)
0.65
5229

ath-miR169i
TAGCCAAGGATGACTTGCCTG (4066)
0.65
5230

ath-miR169j
TAGCCAAGGATGACTTGCCTG (4067)
0.65
5231

ath-miR169k
TAGCCAAGGATGACTTGCCTG (4068)
0.65
5232

ath-miR169l
TAGCCAAGGATGACTTGCCTG (4069)
0.65
5233

ath-
TAGCCAAGGATGACTTGCCTG (4070)
0.65
5234

miR169m

ath-miR169n
TAGCCAAGGATGACTTGCCTG (4071)
0.65
5235

bdi-miR169b
TAGCCAAGGATGACTTGCCGG (4072)
0.6
5236

bdi-miR169d
TAGCCAAGAATGACTTGCCTA (4073)
0.65
5237

bdi-miR169e
TAGCCAAGGATGACTTGCCTG (4074)
0.65
5238

bdi-miR169g
TAGCCAAGGATGACTTGCCTG (4075)
0.65
5239

bdi-miR169h
TAGCCAAGGATGACTTGCCTA (4076)
0.65
5240

bdi-miR169i
CCAGCCAAGAATGGCTTGCCTA (4077)
0.6
5241

bdi-miR169j
TAGCCAGGAATGGCTTGCCTA (4078)
0.6
5242

bdi-miR169k
TAGCCAAGGATGATTTGCCTGT (4079)
0.6
5243

bna-
TAGCCAAGGATGACTTGCCTA (4080)
0.65
5244

miR169c

bna-
TAGCCAAGGATGACTTGCCTA (4081)
0.65
5245

miR169d

bna-
TAGCCAAGGATGACTTGCCTA (4082)
0.65
5246

miR169e

bna-miR169f
TAGCCAAGGATGACTTGCCTA (4083)
0.65
5247

bna-
TAGCCAAGGATGACTTGCCTGC (4084)
0.65
5248

miR169g

bna-
TAGCCAAGGATGACTTGCCTGC (4085)
0.65
5249

miR169h

bna-miR169i
TAGCCAAGGATGACTTGCCTGC (4086)
0.65
5250

bna-miR169j
TAGCCAAGGATGACTTGCCTGC (4087)
0.65
5251

bna-
TAGCCAAGGATGACTTGCCTGC (4088)
0.65
5252

miR169k

bna-miR169l
TAGCCAAGGATGACTTGCCTGC (4089)
0.65
5253

far-miR169
TAGCCAAGGATGACTTGCCTA (4090)
0.65
5254

ghb-
TAGCCAAGGATGACTTGCCTG (4091)
0.65
5255

miR169a

ghr-miR169
ACGCCAAGGATGTCTTGCGTC (4092)
0.6
5256

mtr-miR169f
AAGCCAAGGATGACTTGCCTA (4093)
0.6
5257

osa-miR169e
TAGCCAAGGATGACTTGCCGG (4094)
0.6
5258

osa-miR169f
TAGCCAAGGATGACTTGCCTA (4095)
0.65
5259

osa-miR169g
TAGCCAAGGATGACTTGCCTA (4096)
0.65
5260

osa-miR169h
TAGCCAAGGATGACTTGCCTG (4097)
0.65
5261

osa-miR169i
TAGCCAAGGATGACTTGCCTG (4098)
0.65
5262

osa-miR169j
TAGCCAAGGATGACTTGCCTG (4099)
0.65
5263

osa-miR169k
TAGCCAAGGATGACTTGCCTG (4100)
0.65
5264

osa-miR169l
TAGCCAAGGATGACTTGCCTG (4101)
0.65
5265

osa-
TAGCCAAGGATGACTTGCCTG (4102)
0.65
5266

miR169m

osa-miR169n
TAGCCAAGAATGACTTGCCTA (4103)
0.65
5267

osa-miR169o
TAGCCAAGAATGACTTGCCTA (4104)
0.65
5268

ptc-
TAGCCAAGGACGACTTGCCCA (4105)
0.6
5269

miR169ab

ptc-
TAGCCAAGGACGACTTGCCCA (4106)
0.6
5270

miR169ac

ptc-
TAGCCAAGGACGACTTGCCCA (4107)
0.6
5271

miR169ad

ptc-
TAGCCAAGGACGACTTGCCCA (4108)
0.6
5272

miR169ae

ptc-
TAGCCAAGGACGACTTGCCCA (4109)
0.6
5273

miR169af

ptc-miR169i
TAGCCAAGGATGACTTGCCTG (4110)
0.65
5274

ptc-miR169j
TAGCCAAGGATGACTTGCCTG (4111)
0.65
5275

ptc-miR169k
TAGCCAAGGATGACTTGCCTG (4112)
0.65
5276

ptc-miR169l
TAGCCAAGGATGACTTGCCTG (4113)
0.65
5277

ptc-
TAGCCAAGGATGACTTGCCTG (4114)
0.65
5278

miR169m

ptc-miR169o
AAGCCAAGGATGACTTGCCTG (4115)
0.6
5279

ptc-miR169p
AAGCCAAGGATGACTTGCCTG (4116)
0.6
5280

ptc-miR169q
TAGCCAAGGACGACTTGCCTG (4117)
0.65
5281

ptc-miR169r
TAGCCAAGGATGACTTGCCTA (4118)
0.65
5282

ptc-miR169s
TCAGCCAAGGATGACTTGCCG (4119)
0.65
5283

ptc-miR169u
TAGCCAAGGACGACTTGCCTA (4120)
0.65
5284

ptc-miR169v
TAGCCAAGGATGACTTGCCCA (4121)
0.6
5285

ptc-
TAGCCAAGGATGACTTGCCCA (4122)
0.6
5286

miR169w

sbi-miR169c
TAGCCAAGGATGACTTGCCTA (4123)
0.65
5287

sbi-miR169d
TAGCCAAGGATGACTTGCCTA (4124)
0.65
5288

sbi-miR169e
TAGCCAAGGATGACTTGCCGG (4125)
0.6
5289

sbi-miR169f
TAGCCAAGGATGACTTGCCTG (4126)
0.65
5290

sbi-miR169g
TAGCCAAGGATGACTTGCCTG (4127)
0.65
5291

sbi-miR169h
TAGCCAAGGATGACTTGCCTA (4128)
0.65
5292

sbi-miR169i
TAGCCAAGAATGACTTGCCTA (4129)
0.65
5293

sbi-miR169j
TAGCCAAGGATGACTTGCCGG (4130)
0.6
5294

sbi-miR169l
TAGCCAAGGATGACTTGCCTG (4131)
0.65
5295

sbi-
TAGCCAAGGATGACTTGCCTA (4132)
0.65
5296

miR169m

sbi-miR169n
TAGCCAAGGATGACTTGCCTA (4133)
0.65
5297

sbi-miR169o
TAGCCAAGGATGATTTGCCTG (4134)
0.6
5298

sbi-miR169p
TAGCCAAGAATGGCTTGCCTA (4135)
0.65
5299

sbi-miR169q
TAGCCAAGAATGGCTTGCCTA (4136)
0.65
5300

sly-miR169b
TAGCCAAGGATGACTTGCCTG (4137)
0.65
5301

sly-miR169d
TAGCCAAGGATGACTTGCCTA (4138)
0.65
5302

sof-miR169
TAGCCAAGGATGACTTGCCGG (4139)
0.6
5303

ssp-miR169
TAGCCAAGGATGACTTGCCGG (4140)
0.6
5304

tcc-miR169d
TAGCCAAGGATGACTTGCCTA (4141)
0.65
5305

tcc-miR169f
AAGCCAAGAATGACTTGCCTG (4142)
0.6
5306

tcc-miR169g
TAGCCAGGGATGACTTGCCTA (4143)
0.6
5307

tcc-miR169h
TAGCCAAGGATGACTTGCCTG (4144)
0.65
5308

tcc-miR169i
TAGCCAAGGATGAGTTGCCTG (4145)
0.6
5309

tcc-miR169j
TAGCCAAGGATGACTTGCCTG (4146)
0.65
5310

vvi-miR169e
TAGCCAAGGATGACTTGCCTGC (4147)
0.65
5311

vvi-miR169x
TAGCCAAGGATGACTTGCCTA (4148)
0.65
5312

vvi-miR169y
TAGCGAAGGATGACTTGCCTA (4149)
0.6
5313

zma-
GGCAAGTTGTTCTTGGCTACA (4150)
0.8
5314

miR169a*

zma-
GGCAAGTTGTTCTTGGCTACA (4151)
0.8
5315

miR169b*

zma-
GGCAAGTCTGTCCTTGGCTACA (4152)
0.85
5316

miR169c*

zma-
TAGCCAAGGATGACTTGCCTA (4153)
0.65
5317

miR169f

zma-
GGCATGTCTTCCTTGGCTACT (4154)
0.7
5318

miR169f*

zma-
TAGCCAAGGATGACTTGCCTA (4155)
0.65
5319

miR169g

zma-
TAGCCAAGGATGACTTGCCTA (4156)
0.65
5320

miR169h

zma-
TAGCCAAGGATGACTTGCCTG (4157)
0.65
5321

miR169i

zma-
GGCAGTCTCCTTGGCTAG (4158)
0.65
5322

miR169i*

zma-
TAGCCAAGGATGACTTGCCTG (4159)
0.65
5323

miR169j

zma-
GGCAGTCTCCTTGGCTAG (4160)
0.65
5324

miR169j*

zma-
TAGCCAAGGATGACTTGCCTG (4161)
0.65
5325

miR169k

zma-
GGCAGTCTCCTTGGCTAG (4162)
0.65
5326

miR169k*

zma-
TAGCCAAGAATGGCTTGCCTA (4163)
0.65
5327

miR169m

zma-
TAGCCAAGAATGGCTTGCCTA (4164)
0.65
5328

miR169n

zma-
GGCAGGCCTTCTTGGCTAAG (4165)
0.6
5329

miR169n*

zma-
TAGCCAAGAATGACTTGCCTA (4166)
0.65
5330

miR169o

zma-
GGCAGGTCTTCTTGGCTAGC (4167)
0.65
5331

miR169o*

zma-
TAGCCAAGGATGACTTGCCGG (4168)
0.6
5332

miR169p

zma-
GGCAAGTCATCTGGGGCTACG (4169)
0.6
5333

miR169p*

zma-
TAGCCAAGAATGGCTTGCCTA (4170)
0.65
5334

miR169q

zma-
GGCAAGTTGTCCTTGGCTACA (4171)
0.85
5335

miR169r*

ppt-miR533b-
ppt-
GAGCTGGCCAGGCTGTGAGGG (4172)
0.95
5336

5p
miR533a*

ptc-
aly-
TAATCTGCATCCTGAGGTTTA (4173)
0.95
5337

miRf11953-
miR2111a

akr

aly-
TAATCTGCATCCTGAGGTTTA (4174)
0.95
5338

miR2111b

ath-
TAATCTGCATCCTGAGGTTTA (4175)
0.95
5339

miR2111a

ath-
TAATCTGCATCCTGAGGTTTA (4176)
0.95
5340

miR2111b

bna-
TAATCTGCATCCTGAGGTTTA (4177)
0.95
5341

miR2111a

bna-
TAATCTGCATCCTGAGGTTTA (4178)
0.95
5342

miR2111b-

5p

bra-
TAATCTGCATCCTGAGGTTTA (4179)
0.95
5343

miR2111a

bra-
TAATCTGCATCCTGAGGTTTA (4180)
0.95
5344

miR2111b

lja-miR2111
TAATCTGCATCCTGAGGTTTA (4181)
0.95
5345

mtr-
TAATCTGCATCCTGAGGTTTA (4182)
0.95
5346

miR2111a

mtr-
TAATCTGCATCCTGAGGTTTA (4183)
0.95
5347

miR2111b

mtr-
TAATCTGCATCCTGAGGTTTA (4184)
0.95
5348

miR2111c

mtr-
TAATCTGCATCCTGAGGTTTA (4185)
0.95
5349

miR2111d

mtr-
TAATCTGCATCCTGAGGTTTA (4186)
0.95
5350

miR2111e

mtr-
TAATCTGCATCCTGAGGTTTA (4187)
0.95
5351

miR2111f

mtr-
TAATCTGCATCCTGAGGTTTA (4188)
0.95
5352

miR2111h

mtr-
TAATCTGCATCCTGAGGTTTA (4189)
0.95
5353

miR2111i

mtr-
TAATCTGCATCCTGAGGTTTA (4190)
0.95
5354

miR2111j

mtr-
TAATCTGCATCCTGAGGTTTA (4191)
0.95
5355

miR2111k

mtr-
TAATCTGCATCCTGAGGTTTA (4192)
0.95
5356

miR2111l

mtr-
TAATCTGCATCCTGAGGTTTA (4193)
0.95
5357

miR2111m

mtr-
TAATCTGCATCCTGAGGTTTA (4194)
0.95
5358

miR2111n

mtr-
TAATCTGCATCCTGAGGTTTA (4195)
0.95
5359

miR2111o

mtr-
TAATCTGCATCCTGAGGTTTA (4196)
0.95
5360

miR2111p

mtr-
TAATCTGCATCCTGAGGTTTA (4197)
0.95
5361

miR2111q

mtr-
TAATCTGCATCCTGAGGTTTA (4198)
0.95
5362

miR2111r

mtr-
TAATCTGCATCCTGAGGTTTA (4199)
0.95
5363

miR2111s

tcc-miR2111
TAATCTGCATCCTGAGGTTTA (4200)
0.95
5364

vvi-
TAATCTGCATCCTGAGGTCTA (4201)
0.9
5365

miR2111-5p

vvi-miR2111-
aly-
TAATCTGCATCCTGAGGTTTA (4202)
0.95
5366

5p
miR2111a

aly-
TAATCTGCATCCTGAGGTTTA (4203)
0.95
5367

miR2111b

ath-
TAATCTGCATCCTGAGGTTTA (4204)
0.95
5368

miR2111a

ath-
TAATCTGCATCCTGAGGTTTA (4205)
0.95
5369

miR2111b

bna-
TAATCTGCATCCTGAGGTTTA (4206)
0.95
5370

miR2111a

bna-
TAATCTGCATCCTGAGGTTTA (4207)
0.95
5371

miR2111b-

5p

bra-
TAATCTGCATCCTGAGGTTTA (4208)
0.95
5372

miR2111a

bra-
TAATCTGCATCCTGAGGTTTA (4209)
0.95
5373

miR2111b

lja-miR2111
TAATCTGCATCCTGAGGTTTA (4210)
0.95
5374

mtr-
TAATCTGCATCCTGAGGTTTA (4211)
0.95
5375

miR2111a

mtr-
TAATCTGCATCCTGAGGTTTA (4212)
0.95
5376

miR2111b

mtr-
TAATCTGCATCCTGAGGTTTA (4213)
0.95
5377

miR2111c

mtr-
TAATCTGCATCCTGAGGTTTA (4214)
0.95
5378

miR2111d

mtr-
TAATCTGCATCCTGAGGTTTA (4215)
0.95
5379

miR2111e

mtr-
TAATCTGCATCCTGAGGTTTA (4216)
0.95
5380

miR2111f

mtr-
TAATCTGCATCCTGAGGTTTA (4217)
0.95
5381

miR2111h

mtr-
TAATCTGCATCCTGAGGTTTA (4218)
0.95
5382

miR2111i

mtr-
TAATCTGCATCCTGAGGTTTA (4219)
0.95
5383

miR2111j

mtr-
TAATCTGCATCCTGAGGTTTA (4220)
0.95
5384

miR2111k

mtr-
TAATCTGCATCCTGAGGTTTA (4221)
0.95
5385

miR2111l

mtr-
TAATCTGCATCCTGAGGTTTA (4222)
0.95
5386

miR2111m

mtr-
TAATCTGCATCCTGAGGTTTA (4223)
0.95
5387

miR2111n

mtr-
TAATCTGCATCCTGAGGTTTA (4224)
0.95
5388

miR2111o

mtr-
TAATCTGCATCCTGAGGTTTA (4225)
0.95
5389

miR2111p

mtr-
TAATCTGCATCCTGAGGTTTA (4226)
0.95
5390

miR2111q

mtr-
TAATCTGCATCCTGAGGTTTA (4227)
0.95
5391

miR2111r

mtr-
TAATCTGCATCCTGAGGTTTA (4228)
0.95
5392

miR2111s

tcc-miR2111
TAATCTGCATCCTGAGGTTTA (4229)
0.95
5393

zma-
aly-
GTTCAATAAAGCTGTGGGAAG (4230)
0.95
5394

miR396b-3p
miR396a-3p

aly-
GCTCAAGAAAGCTGTGGGAAA (4231)
0.9
5395

miR396b-3p

csi-miR396c
TTCAAGAAATCTGTGGGAAG (4232)
0.81
5396

gma-
AAGAAAGCTGTGGGAGAATATGGC (4233)
0.71
5397

miR396d

osa-
GTTCAAGAAAGCCCATGGAAA (4234)
0.76
5398

miR396e*

osa-
ATGGTTCAAGAAAGCCCATGGAAA (4235)
0.76
5399

miR396e-3p

osa-
GTTCAAGAAAGTCCTTGGAAA (4236)
0.76
5400

miR396f*

osa-
ATAGTTCAAGAAAGTCCTTGGAAA
0.76
5401

miR396f-3p
(4237)

zma-
GTTCAATAAAGCTGTGGGAAA (4238)
1
5402

miR396a*

zma-
GGTCAAGAAAGCCGTGGGAAG (4239)
0.81
5403

miR396e*

zma-
GGTCAAGAAAGCTGTGGGAAG (4240)
0.86
5404

miR396f*

zma-
GTTCAAGAAAGCTGTGGAAGA (4241)
0.86
5405

miR396g*

ctr-miR171
aly-miR171a
TGATTGAGCCGCGCCAATATC (4242)
0.81
5406

aly-miR171b
TTGAGCCGTGCCAATATCACG (4243)
0.81
5407

aly-miR171c
TTGAGCCGTGCCAATATCACG (4244)
0.81
5408

aqc-miR171a
TGATTGAGCCGTGCCAATATC (4245)
0.76
5409

aqc-
TGATTGAGCCGTGCCAATATC (4246)
0.76
5410

miR171b

aqc-miR171c
TAATTGAACCGCACTAATATC (4247)
0.67
5411

aqc-
TGATTGAGCCGTGCCAATATC (4248)
0.76
5412

miR171d

aqc-miR171e
TGAATGAACCGAGCCAACATC (4249)
0.62
5413

aqc-miR171f
TAATTGAGCCGTGCCAATATC (4250)
0.76
5414

ath-miR171a
TGATTGAGCCGCGCCAATATC (4251)
0.81
5415

ath-miR171b
TTGAGCCGTGCCAATATCACG (4252)
0.81
5416

ath-miR171c
TTGAGCCGTGCCAATATCACG (4253)
0.81
5417

bdi-miR171a
TGATTGAGCCGCGCCAATATC (4254)
0.81
5418

bdi-miR171b
TGATTGAGCCGTGCCAATATC (4255)
0.76
5419

bdi-miR171c
TGATTGAGCCGTGCCAATATC (4256)
0.76
5420

bdi-miR171d
TGATTGAGCCGTGCCAATATC (4257)
0.76
5421

bna-
TTGAGCCGTGCCAATATCACG (4258)
0.81
5422

miR171a

bna-
TTGAGCCGTGCCAATATCACG (4259)
0.81
5423

miR171b

bna-
TTGAGCCGTGCCAATATCACG (4260)
0.81
5424

miR171c

bna-
TTGAGCCGTGCCAATATCACG (4261)
0.81
5425

miR171d

bna-
TTGAGCCGTGCCAATATCACG (4262)
0.81
5426

miR171e

bna-miR171f
TGATTGAGCCGCGCCAATATC (4263)
0.81
5427

bna-
TGATTGAGCCGCGCCAATATCT (4264)
0.86
5428

miR171g

bol-miR171a
TTGAGCCGTGCCAATATCACG (4265)
0.81
5429

bra-miR171a
TTGAGCCGTGCCAATATCACG (4266)
0.81
5430

bra-miR171b
TTGAGCCGTGCCAATATCACG (4267)
0.81
5431

bra-miR171c
TTGAGCCGTGCCAATATCACG (4268)
0.81
5432

bra-miR171d
TTGAGCCGTGCCAATATCACG (4269)
0.81
5433

bra-miR171e
TGATTGAGCCGCGCCAATATC (4270)
0.81
5434

ccl-miR171
TGATTGAGCCGCGCCAATATC (4271)
0.81
5435

crt-miR171
TGATTGAGCCGTGCCAATATC (4272)
0.76
5436

csi-miR171a
TTGAGCCGCGCCAATATCAC (4273)
0.86
5437

csi-miR171b
CGAGCCGAATCAATATCACTC (4274)
0.71
5438

ctr-miR171
TTGAGCCGCGTCAATATCTCC (4275)
1
5439

far-miR171
TGATTGAGCCGTGCCAATATC (4276)
0.76
5440

gma-
TGAGCCGTGCCAATATCACGA (4277)
0.76
5441

miR171a

gma-
CGAGCCGAATCAATATCACTC (4278)
0.71
5442

miR171b-3p

hvu-miR171
TGATTGAGCCGTGCCAATATC (4279)
0.76
5443

mtr-miR171
TGATTGAGTCGTGCCAATATC (4280)
0.71
5444

mtr-
TGATTGAGCCGCGTCAATATC (4281)
0.86
5445

miR171b

mtr-miR171c
TGATTGAGCCGTGCCAATATT (4282)
0.71
5446

mtr-
TGATTGAGCCGTGCCAATATC (4283)
0.76
5447

miR171d

mtr-miR171e
AGATTGAGCCGCGCCAATATC (4284)
0.81
5448

mtr-miR171f
TTGAGCCGTGCCAATATCACG (4285)
0.81
5449

mtr-
TGATTGAGCCGTGCCAATATC (4286)
0.76
5450

miR171g

osa-miR171a
TGATTGAGCCGCGCCAATATC (4287)
0.81
5451

osa-miR171b
TGATTGAGCCGTGCCAATATC (4288)
0.76
5452

osa-miR171c
TGATTGAGCCGTGCCAATATC (4289)
0.76
5453

osa-miR171d
TGATTGAGCCGTGCCAATATC (4290)
0.76
5454

osa-miR171e
TGATTGAGCCGTGCCAATATC (4291)
0.76
5455

osa-miR171f
TGATTGAGCCGTGCCAATATC (4292)
0.76
5456

osa-miR171g
GAGGTGAGCCGAGCCAATATC (4293)
0.71
5457

osa-miR171h
GTGAGCCGAACCAATATCACT (4294)
0.71
5458

osa-miR171i
GGATTGAGCCGCGTCAATATC (4295)
0.86
5459

ppt-miR171a
TGAGCCGCGCCAATATCACAT (4296)
0.81
5460

ppt-miR171b
TTGAGCCGCGCCAATATCACA (4297)
0.86
5461

pta-miR171
TGATTGAGACGAGTCCATATC (4298)
0.71
5462

ptc-miR171a
TTGAGCCGTGCCAATATCACG (4299)
0.81
5463

ptc-miR171b
TTGAGCCGTGCCAATATCACG (4300)
0.81
5464

ptc-miR171c
AGATTGAGCCGCGCCAATATC (4301)
0.81
5465

ptc-miR171d
AGATTGAGCCGCGCCAATATC (4302)
0.81
5466

ptc-miR171e
TGATTGAGCCGTGCCAATATC (4303)
0.76
5467

ptc-miR171f
TGATTGAGCCGTGCCAATATC (4304)
0.76
5468

ptc-miR171g
TGATTGAGCCGTGCCAATATC (4305)
0.76
5469

ptc-miR171h
TGATTGAGCCGTGCCAATATC (4306)
0.76
5470

ptc-miR171i
TGATTGAGCCGTGCCAATATC (4307)
0.76
5471

ptc-miR171j
GGATTGAGCCGCGCCAATACT (4308)
0.71
5472

ptc-miR171k
GGATTGAGCCGCGCCAATATC (4309)
0.81
5473

ptc-miR171l
CGAGCCGAATCAATATCACT (4310)
0.71
5474

ptc-
CGAGCCGAATCAATATCACT (4311)
0.71
5475

miR171m

ptc-miR171n
CGAGCCGAATCAATATCACT (4312)
0.71
5476

rco-miR171a
TTGAGCCGTGCCAATATCACG (4313)
0.81
5477

rco-miR171b
TTGAGCCGTGCCAATATCACG (4314)
0.81
5478

rco-miR171c
TGATTGAGCCGTGCCAATATC (4315)
0.76
5479

rco-miR171d
TGATTGAGCCGTGCCAATATC (4316)
0.76
5480

rco-miR171e
TGATTGAGCCGTGCCAATATC (4317)
0.76
5481

rco-miR171f
TGATTGAGCCGTGCCAATATC (4318)
0.76
5482

rco-miR171g
AGATTGAGCCGCGCCAATATC (4319)
0.81
5483

sbi-miR171a
TGATTGAGCCGTGCCAATATC (4320)
0.76
5484

sbi-miR171b
TGATTGAGCCGTGCCAATATC (4321)
0.76
5485

sbi-miR171c
GAGGTGAGCCGAGCCAATATC (4322)
0.71
5486

sbi-miR171d
TGATTGAGCCGTGCCAATATC (4323)
0.76
5487

sbi-miR171e
GTGAGCCGAACCAATATCACT (4324)
0.71
5488

sbi-miR171f
ATGAGCCGAACCAATATCACT (4325)
0.71
5489

sbi-miR171g
TGATTGAGCCGCGCCAATATC (4326)
0.81
5490

sbi-miR171h
GGATTGAGCCGCGTCAATATC (4327)
0.86
5491

sbi-miR171i
TGATTGAGCCGTGCCAATATC (4328)
0.76
5492

sbi-miR171j
TGATTGAGCCGCGCCAATATC (4329)
0.81
5493

sbi-miR171k
TGATTGAGCCGTGCCAATATC (4330)
0.76
5494

sly-miR171a
TGATTGAGCCGTGCCAATATC (4331)
0.76
5495

sly-miR171b
TTGAGCCGTGCCAATATCACG (4332)
0.81
5496

sly-miR171d
TTGAGCCGCGCCAATATCAC (4333)
0.86
5497

smo-
TTGAGCCGTGCCAATATCACT (4334)
0.81
5498

miR171a

smo-
TGAGCCGTGCCAATATCACAT (4335)
0.76
5499

miR171b

smo-
TTGAGTCGCGCCAATATCATG (4336)
0.76
5500

miR171c

smo-
TGAGCCGCGCCAATATCACAT (4337)
0.81
5501

miR171d

tae-miR171a
TGATTGAGCCGTGCCAATATC (4338)
0.76
5502

tae-miR171b
TTGAGCCGTGCCAATATCACG (4339)
0.81
5503

tcc-miR171a
TGATTGAGCCGCGCCAATATC (4340)
0.81
5504

tcc-miR171b
AGATTGAGCCGCGCCAATATC (4341)
0.81
5505

tcc-miR171c
AGATTGAGCCGCGCCAATATC (4342)
0.81
5506

tcc-miR171d
TGATTGAGCCGTGCCAATATC (4343)
0.76
5507

tcc-miR171e
TGATTGAGCCGTGCCAATATC (4344)
0.76
5508

tcc-miR171f
TGATTGAGCCGTGCCAATATC (4345)
0.76
5509

tcc-miR171g
TGATTGAGCCGTGCCAATATC (4346)
0.76
5510

tcc-miR171h
TGATTGAGCCGTGCCAATATC (4347)
0.76
5511

vvi-miR171a
TGATTGAGCCGTGCCAATATC (4348)
0.76
5512

vvi-miR171b
TGATTGAGCCGCGTCAATATC (4349)
0.86
5513

vvi-miR171c
TGATTGAGCCGTGCCAATATC (4350)
0.76
5514

vvi-miR171d
TGATTGAGCCGTGCCAATATC (4351)
0.76
5515

vvi-miR171e
TGATTGAGCCGCGCCAATATC (4352)
0.81
5516

vvi-miR171f
TTGAGCCGCGCCAATATCACT (4353)
0.86
5517

vvi-miR171g
TTGAGCCGAACCAATATCACC (4354)
0.81
5518

vvi-miR171h
TGGTTGAGCCGCGCCAATATC (4355)
0.81
5519

vvi-miR171i
TGATTGAGCCGTGCCAATATC (4356)
0.76
5520

zma-
TGATTGAGCCGCGCCAATAT (4357)
0.76
5521

miR171a

zma-
TTGAGCCGTGCCAATATCAC (4358)
0.81
5522

miR171b

zma-
TGACTGAGCCGTGCCAATATC (4359)
0.71
5523

miR171c

zma-
TGATTGAGCCGTGCCAATATC (4360)
0.76
5524

miR171d

zma-
TGATTGAGCCGTGCCAATATC (4361)
0.76
5525

miR171e

zma-
TTGAGCCGTGCCAATATCACA (4362)
0.81
5526

miR171f

zma-
GAGGTGAGCCGAGCCAATATC (4363)
0.71
5527

miR171g

zma-
GTGAGCCGAACCAATATCACT (4364)
0.71
5528

miR171h

zma-
TGATTGAGCCGTGCCAATATC (4365)
0.76
5529

miR171i

zma-
TGATTGAGCCGTGCCAATATC (4366)
0.76
5530

miR171j

zma-
GTGAGCCGAACCAATATCACT (4367)
0.71
5531

miR171k

zma-
GGATTGAGCCGCGTCAATATC (4368)
0.86
5532

miR171l

zma-
GGATTGAGCCGCGTCAATATC (4369)
0.86
5533

miR171m

zma-
TGATTGAGCCGCGCCAATATC (4370)
0.81
5534

miR171n

aly-miR160c-
ahy-miR160-
GCATGAAGGGAGTCACGCAGG (4371)
0.67
5535

3p
3p

aly-
GCGTATGAGGAGCCATGCATA (4372)
0.81
5536

miR160a*

aly-
GCGTACAGAGTAGTCAAGCATG (4373)
0.86
5537

miR160b*

bra-
GCGTATGAGGAGCCATGCATA (4374)
0.81
5538

miR160a-3p

zma-
GCGTGCAAGGGGCCAAGCATG (4375)
0.9
5539

miR160a*

zma-
GCGTGCAAGGAGCCAAGCATG (4376)
0.95
5540

miR160b*

zma-
GCGTGCATGGTGCCAAGCATA (4377)
0.81
5541

miR160c*

zma-
GCGTGCGTGGAGCCAAGCATG (4378)
0.86
5542

miR160d*

zma-
GCGTGCGAGGTGCCAGGCATG (4379)
0.81
5543

miR160f*

zma-
GCGTGCAAGGAGCCAAGCATG (4380)
0.95
5544

miR160g*

bra-miR160a-
aly-
GCGTATGAGGAGCCATGCATA (4381)
1
5545

3p
miR160a*

aly-
GCGTACAGAGTAGTCAAGCATG (4382)
0.76
5546

miR160b*

aly-
GCGTACAAGGAGCCAAGCATG (4383)
0.81
5547

miR160c-3p

zma-
GCGTGCAAGGGGCCAAGCATG (4384)
0.71
5548

miR160a*

zma-
GCGTGCAAGGAGCCAAGCATG (4385)
0.76
5549

miR160b*

zma-
GCGTGCATGGTGCCAAGCATA (4386)
0.71
5550

miR160c*

zma-
GCGTGCGTGGAGCCAAGCATG (4387)
0.76
5551

miR160d*

zma-
GCGTGCGAGGTGCCAGGCATG (4388)
0.76
5552

miR160f*

zma-
GCGTGCAAGGAGCCAAGCATG (4389)
0.76
5553

miR160g*

gma-
ahy-
CCTCGTTCCATACATCATCTA (4390)
0.77
5554

miR1507a
miR1507

gma-
TCTCATTCCATACATCGTCTG (4391)
0.95
5555

miR1507b

gso-
TCTCATTCCATACATCGTCTGA (4392)
1
5556

miR1507a

gso-
TCTCATTCCATACATCGTCTGA (4393)
1
5557

miR1507b

mtr-
CCTCGTTCCATACATCATCTAG (4394)
0.77
5558

miR1507

vun-
TCTCATTCCATACATCGTCTG (4395)
0.95
5559

miR1507a

vun-
TCTCATTCCATACATCGTCTG (4396)
0.95
5560

miR1507b

gma-
gma-
AAGTGATGACATGACAAGCGAAGT (4397)
0.75
5561

miR4371b
miR4371a

gso-miR482a
aqc-miR482a
TCTTGCCGACTCCTCCCATACC (4398)
0.86
5562

aqc-
TCTTGCCGACTCCTCCCATACC (4399)
0.86
5563

miR482b

aqc-miR482c
TCTTGCCGACTCCTCCCATACC (4400)
0.86
5564

csi-miR482a
TCTTCCCTATGCCTCCCATTCC (4401)
0.86
5565

csi-miR482b
TCTTGCCCACCCCTCCCATTCC (4402)
0.81
5566

csi-miR482c
TTCCCTAGTCCCCCTATTCCTA (4403)
0.67
5567

ghr-miR482a
TCTTTCCTACTCCTCCCATACC (4404)
0.9
5568

ghr-miR482b
TCTTGCCTACTCCACCCATGCC (4405)
0.81
5569

gma-miR482
TCTTCCCAATTCCGCCCATTCCTA (4406)
0.76
5570

gma-
TCTTCCCAATTCCGCCCATTCCTA (4407)
0.76
5571

miR482a-3p

gra-miR482
TCTTTCCAATTCCTCCCATTCC (4408)
0.76
5572

gso-
TCTTCCCTACACCTCCCATAC (4409)
1
5573

miR482b

mdm-
TCTTCCCAAGCCCGCCCATTCC (4410)
0.76
5574

miR482

mdo-miR482
TCTTCCCAAGCCCGCCCATTCC (4411)
0.76
5575

pab-
TCTTCCCTACTCCTCCCATTCC (4412)
0.9
5576

miR482a

pab-
TCTTCCCTATTCCTCCCATTCC (4413)
0.86
5577

miR482b

pab-
TCTTTCCTACTCCTCCCATTCC (4414)
0.86
5578

miR482c

pta-miR482a
TCTTCCCTACTCCTCCCATTCC (4415)
0.9
5579

pta-miR482b
TCTTCCCTACTCCTCCCATTCC (4416)
0.9
5580

pta-miR482c
TCTTCCCTATTCCTCCCATT (4417)
0.81
5581

pta-miR482d
TCCTCCCTACTCCTCCCATT (4418)
0.81
5582

ptc-
CCTACTCCTCCCATTCC (4419)
0.67
5583

miR482.1

ptc-
TCTTGCCTACTCCTCCCATT (4420)
0.81
5584

miR482.2

pvu-miR482
TCTTCCCAATTCCGCCCATTCC (4421)
0.76
5585

sly-miR482
TTTCCAATTCCACCCATTCCTA (4422)
0.62
5586

vvi-miR482
TCTTTCCTACTCCTCCCATTCC (4423)
0.86
5587

zma-miR482
TCTTCCTTGTTCCTCCCATT (4424)
0.71
5588

osa-
osa-
AGTGAGGAGGCCGGGGCCGCT (4425)
0.75
5589

miR1846e
miR1846a-

5p

osa-
AGTGAGGAGGCCGGGGCCGCT (4426)
0.75
5590

miR1846b-

5p

osa-
AGTGAGGAGGCCGGGGCCGCT (4427)
0.75
5591

miR1846c-

5p

ppt-miR166m
aly-miR166a
TCGGACCAGGCTTCATTCCCC (4428)
0.86
5592

aly-miR166b
TCGGACCAGGCTTCATTCCCC (4429)
0.86
5593

aly-miR166c
TCGGACCAGGCTTCATTCCCC (4430)
0.86
5594

aly-miR166d
TCGGACCAGGCTTCATTCCCC (4431)
0.86
5595

aly-miR166e
TCGGACCAGGCTTCATTCCCC (4432)
0.86
5596

aly-miR166f
TCGGACCAGGCTTCATTCCCC (4433)
0.86
5597

aly-miR166g
TCGGACCAGGCTTCATTCCCC (4434)
0.86
5598

aqc-miR166a
TCGGACCAGGCTTCATTCCTC (4435)
0.9
5599

aqc-
TCGGACCAGGCTTCATTCCCC (4436)
0.86
5600

miR166b

aqc-miR166c
TCGGACCAGGCTTCATTCCT (4437)
0.9
5601

aqc-
TCGGACCAGGCTTCATTCCTC (4438)
0.9
5602

miR166d

aqc-miR166e
TCGGACCAGGCTTCATTCCCC (4439)
0.86
5603

ath-miR166a
TCGGACCAGGCTTCATTCCCC (4440)
0.86
5604

ath-miR166b
TCGGACCAGGCTTCATTCCCC (4441)
0.86
5605

ath-miR166c
TCGGACCAGGCTTCATTCCCC (4442)
0.86
5606

ath-miR166d
TCGGACCAGGCTTCATTCCCC (4443)
0.86
5607

ath-miR166e
TCGGACCAGGCTTCATTCCCC (4444)
0.86
5608

ath-miR166f
TCGGACCAGGCTTCATTCCCC (4445)
0.86
5609

ath-miR166g
TCGGACCAGGCTTCATTCCCC (4446)
0.86
5610

bdi-miR166
TCGGACCAGGCTTCATTCCCC (4447)
0.86
5611

bdi-miR166a
TCGGACCAGGCTTCATTCCCC (4448)
0.86
5612

bdi-miR166b
TCGGACCAGGCTTCATTCCCC (4449)
0.86
5613

bdi-miR166c
TCGGACCAGGCTTCATTCCCC (4450)
0.86
5614

bdi-miR166d
TCGGACCAGGCTTCATTCCCC (4451)
0.86
5615

bdi-miR166e
CTCGGACCAGGCTTCATTCCC (4452)
0.86
5616

bdi-miR166f
TCTCGGACCAGGCTTCATTCC (4453)
0.86
5617

bna-
TCGGACCAGGCTTCATTCCCC (4454)
0.86
5618

miR166a

bna-
TCGGACCAGGCTTCATTCCCC (4455)
0.86
5619

miR166b

bna-
TCGGACCAGGCTTCATTCCCC (4456)
0.86
5620

miR166c

bna-
TCGGACCAGGCTTCATTCCCC (4457)
0.86
5621

miR166d

cpt-miR166
TCGGACCAGGCTTCATTCCC (4458)
0.86
5622

crt-miR166a
TCGGACCAGGCTTCATTCCCGT (4459)
0.86
5623

crt-miR166b
TCGGACCAGGCTTCATTCCCTT (4460)
0.9
5624

csi-miR166
TCGGACCAGGCTTCATTCCCC (4461)
0.86
5625

csi-miR166a
TCGGACCAGGCTTCATTCCCCC (4462)
0.86
5626

csi-miR166b
TCGGACCAGGCTTCATTCCCGT (4463)
0.86
5627

csi-miR166c
TCGGACCAGGCTTCATTCCC (4464)
0.86
5628

csi-miR166d
TCGGACCAGGCTTCATTCCCT (4465)
0.9
5629

csi-miR166e
TCGGACCAGGCTTCATTCCCC (4466)
0.86
5630

ctr-miR166
TCGGACCAGGCTTCATTCCCCC (4467)
0.86
5631

far-miR166
CCGGACCAGGCTTCATCCCAG (4468)
0.76
5632

flm-miR166
TCGGACCAGGCTTCATCCCCC (4469)
0.81
5633

ghr-miR166a
TCGGACCAGGCTTCATTCCCC (4470)
0.86
5634

ghr-miR166b
TCGGACCAGGCTTCATTCCCC (4471)
0.86
5635

gma-
TCGGACCAGGCTTCATTCCCC (4472)
0.86
5636

miR166a

gma-
TCGGACCAGGCTTCATTCCCC (4473)
0.86
5637

miR166b

gma-
TCGGACCAGGCTTCATTCCCC (4474)
0.86
5638

miR166n

gma-
TCGGACCAGGCTTCATTCCCC (4475)
0.86
5639

miR166o

gma-
TCGGACCAGGCTTCATTCCCG (4476)
0.86
5640

miR166q

gma-
TCGGACCAGGCTTCATTCCCT (4477)
0.9
5641

miR166r

hvu-miR166
TCGGACCAGGCTTCATTCCCC (4478)
0.86
5642

hvu-
TCGGACCAGGCTTCATTCCCC (4479)
0.86
5643

miR166b

hvu-
TCGGACCAGGCTTCATTCCCC (4480)
0.86
5644

miR166c

hvv-miR166
TCGGACCAGGCTTCATTCCCC (4481)
0.86
5645

ini-miR166
TCGGACCAGGCTTCATTCCTC (4482)
0.9
5646

mtr-miR166
TCGGACCAGGCTTCATTCCCC (4483)
0.86
5647

mtr-
TCGGACCAGGCTTCATTCCTA (4484)
0.9
5648

miR166b
(TCGGACCAGGCTTCATTCCCC (5115)

mtr-miR166c
TCGGACCAGGCTTCATTCCTC (4485)
0.9
5649

mtr-
TCGGGCCAGGCTTCATCCCCC (4486)
0.76
5650

miR166d

mtr-miR166e
TCGGACCAGGCTTCATTCCCC (4487)
0.86
5651

mtr-miR166f
TCGGACCAGGCTTCATTCCTC (4488)
0.9
5652

mtr-
TCGGACCAGGCTTCATTCCCC (4489)
0.86
5653

miR166g

mtr-
TCGGACCAGGCTTCATTCCCC (4490)
0.86
5654

miR166h

nsy-miR166
TCGGACCAGGCTTCATTCCCC (4491)
0.86
5655

osa-miR166a
TCGGACCAGGCTTCATTCCCC (4492)
0.86
5656

osa-miR166b
TCGGACCAGGCTTCATTCCCC (4493)
0.86
5657

osa-miR166c
TCGGACCAGGCTTCATTCCCC (4494)
0.86
5658

osa-miR166d
TCGGACCAGGCTTCATTCCCC (4495)
0.86
5659

osa-miR166e
TCGAACCAGGCTTCATTCCCC (4496)
0.81
5660

osa-miR166f
TCGGACCAGGCTTCATTCCCC (4497)
0.86
5661

osa-miR166g
TCGGACCAGGCTTCATTCCTC (4498)
0.9
5662

osa-miR166h
TCGGACCAGGCTTCATTCCTC (4499)
0.9
5663

osa-miR166i
TCGGATCAGGCTTCATTCCTC (4500)
0.86
5664

osa-miR166j
TCGGATCAGGCTTCATTCCTC (4501)
0.86
5665

osa-miR166k
TCGGACCAGGCTTCAATCCCT (4502)
0.86
5666

osa-miR166l
TCGGACCAGGCTTCAATCCCT (4503)
0.86
5667

osa-
TCGGACCAGGCTTCATTCCCT (4504)
0.9
5668

miR166m

osa-miR166n
TCGGACCAGGCTTCATTCCCC (4505)
0.86
5669

pab-
TCGGACCAGGCTTCATTCCTC (4506)
0.9
5670

miR166a

pab-
TCGGACCAGGCTTCATTCCTT (4507)
0.95
5671

miR166b

pga-miR166
TCGGACCAGGCTTCATTCCTT (4508)
0.95
5672

ppt-miR166a
TCGGACCAGGCTTCATTCCCC (4509)
0.86
5673

ppt-miR166b
TCGGACCAGGCTTCATTCCCC (4510)
0.86
5674

ppt-miR166c
TCGGACCAGGCTTCATTCCCC (4511)
0.86
5675

ppt-miR166d
TCGGACCAGGCTTCATTCCCC (4512)
0.86
5676

ppt-miR166e
TCGGACCAGGCTTCATTCCCC (4513)
0.86
5677

ppt-miR166f
TCGGACCAGGCTTCATTCCCC (4514)
0.86
5678

ppt-miR166g
TCGGACCAGGCTTCATTCCCC (4515)
0.86
5679

ppt-miR166h
TCGGACCAGGCTTCATTCCCC (4516)
0.86
5680

ppt-miR166i
TCGGACCAGGCTTCATTCCCC (4517)
0.86
5681

ppt-miR166j
TCCGGACCAGGCTTCATTCCC (4518)
0.81
5682

ppt-miR166k
TCCGGACCAGGCTTCATTCCC (4519)
0.81
5683

ppt-miR166l
TCCGGACCAGGCTTCATTCCC (4520)
0.81
5684

pta-miR166a
TCGGACCAGGCTTCATTCCCC (4521)
0.86
5685

pta-miR166b
TCGGACCAGGCTTCATTCCCC (4522)
0.86
5686

pta-miR166c
CCGGACCAGGCTTCATCCCAG (4523)
0.76
5687

ptc-miR166a
TCGGACCAGGCTTCATTCCCC (4524)
0.86
5688

ptc-miR166b
TCGGACCAGGCTTCATTCCCC (4525)
0.86
5689

ptc-miR166c
TCGGACCAGGCTTCATTCCCC (4526)
0.86
5690

ptc-miR166d
TCGGACCAGGCTTCATTCCCC (4527)
0.86
5691

ptc-miR166e
TCGGACCAGGCTTCATTCCCC (4528)
0.86
5692

ptc-miR166f
TCGGACCAGGCTTCATTCCCC (4529)
0.86
5693

ptc-miR166g
TCGGACCAGGCTTCATTCCCC (4530)
0.86
5694

ptc-miR166h
TCGGACCAGGCTTCATTCCCC (4531)
0.86
5695

ptc-miR166i
TCGGACCAGGCTTCATTCCCC (4532)
0.86
5696

ptc-miR166j
TCGGACCAGGCTTCATTCCCC (4533)
0.86
5697

ptc-miR166k
TCGGACCAGGCTTCATTCCCC (4534)
0.86
5698

ptc-miR166l
TCGGACCAGGCTTCATTCCCC (4535)
0.86
5699

ptc-
TCGGACCAGGCTTCATTCCCC (4536)
0.86
5700

miR166m

ptc-miR166n
TCGGACCAGGCTTCATTCCTT (4537)
0.95
5701

ptc-miR166o
TCGGACCAGGCTTCATTCCTT (4538)
0.95
5702

ptc-miR166p
TCGGACCAGGCTCCATTCCTT (4539)
0.9
5703

ptc-miR166q
TCGGACCAGGCTTCATTCCTT (4540)
0.95
5704

pvu-miR166
TCGGACCAGGCTTCATTCCCC (4541)
0.86
5705

pvu-
TCGGACCAGGCTTCATTCCCC (4542)
0.86
5706

miR166a

rco-miR166a
TCGGACCAGGCTTCATTCCCC (4543)
0.86
5707

rco-miR166b
TCGGACCAGGCTTCATTCCCC (4544)
0.86
5708

rco-miR166c
TCGGACCAGGCTTCATTCCCC (4545)
0.86
5709

rco-miR166d
TCGGACCAGGCTTCATTCCCC (4546)
0.86
5710

rco-miR166e
TCGGACCAGGCTTCATTCCCC (4547)
0.86
5711

sbi-miR166a
TCGGACCAGGCTTCATTCCC (4548)
0.86
5712

sbi-miR166b
TCGGACCAGGCTTCATTCCC (4549)
0.86
5713

sbi-miR166c
TCGGACCAGGCTTCATTCCC (4550)
0.86
5714

sbi-miR166d
TCGGACCAGGCTTCATTCCC (4551)
0.86
5715

sbi-miR166e
TCGGACCAGGCTTCAATCCCT (4552)
0.86
5716

sbi-miR166f
TCGGACCAGGCTTCATTCCTC (4553)
0.9
5717

sbi-miR166g
TCGGACCAGGCTTCAATCCCT (4554)
0.86
5718

sbi-miR166h
TCGGACCAGGCTTCATTCCC (4555)
0.86
5719

sbi-miR166i
TCGGACCAGGCTTCATTCCC (4556)
0.86
5720

sbi-miR166j
TCGGACCAGGCTTCATTCCC (4557)
0.86
5721

sbi-miR166k
TCGGACCAGGCTTCATTCCT (4558)
0.9
5722

sly-miR166a
TCGGACCAGGCTTCATTCCCC (4559)
0.86
5723

sly-miR166b
TCGGACCAGGCTTCATTCCCC (4560)
0.86
5724

smo-
TCGGACCAGGCTTCATTCCCC (4561)
0.86
5725

miR166a

smo-
TCGGACCAGGCTTCATTCCCC (4562)
0.86
5726

miR166b

smo-
TCGGACCAGGCTTCATTCCCC (4563)
0.86
5727

miR166c

sof-miR166
TCGGACCAGGCTTCATTCCCC (4564)
0.86
5728

tae-miR166
CCGGACCAGGCTTCATTCCCA (4565)
0.81
5729

tcc-miR166a
TCGGACCAGGCTTCATTCCCC (4566)
0.86
5730

tcc-miR166b
TCGGACCAGGCTTCATTCCC (4567)
0.86
5731

tcc-miR166c
TCGGACCAGGCTTCATTCCTC (4568)
0.9
5732

tcc-miR166d
TCGGACCAGGCTTCATTCCCC (4569)
0.86
5733

vvi-miR166a
TCGGACCAGGCTTCATTCC (4570)
0.86
5734

vvi-miR166b
TCGGACCAGGCTTCATTCC (4571)
0.86
5735

vvi-miR166c
TCGGACCAGGCTTCATTCCCC (4572)
0.86
5736

vvi-miR166d
TCGGACCAGGCTTCATTCCCC (4573)
0.86
5737

vvi-miR166e
TCGGACCAGGCTTCATTCCCC (4574)
0.86
5738

vvi-miR166f
TCGGACCAGGCTTCATTCCCC (4575)
0.86
5739

vvi-miR166g
TCGGACCAGGCTTCATTCCCC (4576)
0.86
5740

vvi-miR166h
TCGGACCAGGCTTCATTCCCC (4577)
0.86
5741

zma-
TCGGACCAGGCTTCATTCCCC (4578)
0.86
5742

miR166a

zma-
TCGGACCAGGCTTCATTCCC (4579)
0.86
5743

miR166b

zma-
TCGGACCAGGCTTCATTCCC (4580)
0.86
5744

miR166c

zma-
TCGGACCAGGCTTCATTCCC (4581)
0.86
5745

miR166d

zma-
TCGGACCAGGCTTCATTCCC (4582)
0.86
5746

miR166e

zma-
TCGGACCAGGCTTCATTCCC (4583)
0.86
5747

miR166f

zma-
TCGGACCAGGCTTCATTCCC (4584)
0.86
5748

miR166g

zma-
TCGGACCAGGCTTCATTCCC (4585)
0.86
5749

miR166h

zma-
TCGGACCAGGCTTCATTCCC (4586)
0.86
5750

miR166i

zma-
TCGGACCAGGCTTCAATCCCT (4587)
0.86
5751

miR166j

zma-
TCGGACCAGGCTTCAATCCCT (4588)
0.86
5752

miR166k

zma-
TCGGACCAGGCTTCATTCCTC (4589)
0.9
5753

miR166l

zma-
TCGGACCAGGCTTCATTCCTC (4590)
0.9
5754

miR166m

zma-
TCGGACCAGGCTTCAATCCCT (4591)
0.86
5755

miR166n

zma-
TCGGACCAGGCTTCATTCCCC (4592)
0.86
5756

miR166o

zma-
TCGGACCAGGCTTCATTCCCC (4593)
0.86
5757

miR166p

zma-
TCGGACCAGGCTTCATTCCCC (4594)
0.86
5758

miR166q

zma-
TCGGACCAGGCTTCATTCCCC (4595)
0.86
5759

miR166r

zma-
TCGGACCAGGCTTCATTCCCC (4596)
0.86
5760

miR166s

zma-
TCGGACCAGGCTTCATTCCCC (4597)
0.86
5761

miR166t

zma-
TCGGACCACGCTTCATTCCCC (4598)
0.81
5762

miR166u

pta-miR166c
aly-miR166a
TCGGACCAGGCTTCATTCCCC (4599)
0.81
5763

aly-miR166b
TCGGACCAGGCTTCATTCCCC (4600)
0.81
5764

aly-miR166c
TCGGACCAGGCTTCATTCCCC (4601)
0.81
5765

aly-miR166d
TCGGACCAGGCTTCATTCCCC (4602)
0.81
5766

aly-miR166e
TCGGACCAGGCTTCATTCCCC (4603)
0.81
5767

aly-miR166f
TCGGACCAGGCTTCATTCCCC (4604)
0.81
5768

aly-miR166g
TCGGACCAGGCTTCATTCCCC (4605)
0.81
5769

aqc-miR166a
TCGGACCAGGCTTCATTCCTC (4606)
0.81
5770

aqc-
TCGGACCAGGCTTCATTCCCC (4607)
0.81
5771

miR166b

aqc-miR166c
TCGGACCAGGCTTCATTCCT (4608)
0.81
5772

aqc-
TCGGACCAGGCTTCATTCCTC (4609)
0.81
5773

miR166d

aqc-miR166e
TCGGACCAGGCTTCATTCCCC (4610)
0.81
5774

ath-miR166a
TCGGACCAGGCTTCATTCCCC (4611)
0.81
5775

ath-miR166b
TCGGACCAGGCTTCATTCCCC (4612)
0.81
5776

ath-miR166c
TCGGACCAGGCTTCATTCCCC (4613)
0.81
5777

ath-miR166d
TCGGACCAGGCTTCATTCCCC (4614)
0.81
5778

ath-miR166e
TCGGACCAGGCTTCATTCCCC (4615)
0.81
5779

ath-miR166f
TCGGACCAGGCTTCATTCCCC (4616)
0.81
5780

ath-miR166g
TCGGACCAGGCTTCATTCCCC (4617)
0.81
5781

bdi-miR166
TCGGACCAGGCTTCATTCCCC (4618)
0.81
5782

bdi-miR166a
TCGGACCAGGCTTCATTCCCC (4619)
0.81
5783

bdi-miR166b
TCGGACCAGGCTTCATTCCCC (4620)
0.81
5784

bdi-miR166c
TCGGACCAGGCTTCATTCCCC (4621)
0.81
5785

bdi-miR166d
TCGGACCAGGCTTCATTCCCC (4622)
0.81
5786

bdi-miR166e
CTCGGACCAGGCTTCATTCCC (4623)
0.81
5787

bdi-miR166f
TCTCGGACCAGGCTTCATTCC (4624)
0.81
5788

bna-
TCGGACCAGGCTTCATTCCCC (4625)
0.81
5789

miR166a

bna-
TCGGACCAGGCTTCATTCCCC (4626)
0.81
5790

miR166b

bna-
TCGGACCAGGCTTCATTCCCC (4627)
0.81
5791

miR166c

bna-
TCGGACCAGGCTTCATTCCCC (4628)
0.81
5792

miR166d

cpt-miR166
TCGGACCAGGCTTCATTCCC (4629)
0.81
5793

crt-miR166a
TCGGACCAGGCTTCATTCCCGT (4630)
0.86
5794

crt-miR166b
TCGGACCAGGCTTCATTCCCTT (4631)
0.81
5795

csi-miR166
TCGGACCAGGCTTCATTCCCC (4632)
0.81
5796

csi-miR166a
TCGGACCAGGCTTCATTCCCCC (4633)
0.81
5797

csi-miR166b
TCGGACCAGGCTTCATTCCCGT (4634)
0.86
5798

csi-miR166c
TCGGACCAGGCTTCATTCCC (4635)
0.81
5799

csi-miR166d
TCGGACCAGGCTTCATTCCCT (4636)
0.81
5800

csi-miR166e
TCGGACCAGGCTTCATTCCCC (4637)
0.81
5801

ctr-miR166
TCGGACCAGGCTTCATTCCCCC (4638)
0.81
5802

far-miR166
CCGGACCAGGCTTCATCCCAG (4639)
1
5803

flm-miR166
TCGGACCAGGCTTCATCCCCC (4640)
0.86
5804

ghr-miR166a
TCGGACCAGGCTTCATTCCCC (4641)
0.81
5805

ghr-miR166b
TCGGACCAGGCTTCATTCCCC (4642)
0.81
5806

gma-
TCGGACCAGGCTTCATTCCCC (4643)
0.81
5807

miR166a

gma-
TCGGACCAGGCTTCATTCCCC (4644)
0.81
5808

miR166b

gma-
TCGGACCAGGCTTCATTCCCC (4645)
0.81
5809

miR166n

gma-
TCGGACCAGGCTTCATTCCCC (4646)
0.81
5810

miR166o

gma-
TCGGACCAGGCTTCATTCCCG (4647)
0.86
5811

miR166q

gma-
TCGGACCAGGCTTCATTCCCT (4648)
0.81
5812

miR166r

hvu-miR166
TCGGACCAGGCTTCATTCCCC (4649)
0.81
5813

hvu-
TCGGACCAGGCTTCATTCCCC (4650)
0.81
5814

miR166b

hvu-
TCGGACCAGGCTTCATTCCCC (4651)
0.81
5815

miR166c

hvv-miR166
TCGGACCAGGCTTCATTCCCC (4652)
0.81
5816

ini-miR166
TCGGACCAGGCTTCATTCCTC (4653)
0.81
5817

mtr-miR166
TCGGACCAGGCTTCATTCCCC (4654)
0.81
5818

mtr-
TCGGACCAGGCTTCATTCCTA (4655)
0.81
5819

miR166b

mtr-miR166c
TCGGACCAGGCTTCATTCCTC (4656)
0.81
5820

mtr-
TCGGGCCAGGCTTCATCCCCC (4657)
0.81
5821

miR166d

mtr-miR166e
TCGGACCAGGCTTCATTCCCC (4658)
0.81
5822

mtr-miR166f
TCGGACCAGGCTTCATTCCTC (4659)
0.81
5823

mtr-
TCGGACCAGGCTTCATTCCCC (4660)
0.81
5824

miR166g

mtr-
TCGGACCAGGCTTCATTCCCC (4661)
0.81
5825

miR166h

nsy-miR166
TCGGACCAGGCTTCATTCCCC (4662)
0.81
5826

osa-miR166a
TCGGACCAGGCTTCATTCCCC (4663)
0.81
5827

osa-miR166b
TCGGACCAGGCTTCATTCCCC (4664)
0.81
5828

osa-miR166c
TCGGACCAGGCTTCATTCCCC (4665)
0.81
5829

osa-miR166d
TCGGACCAGGCTTCATTCCCC (4666)
0.81
5830

osa-miR166e
TCGAACCAGGCTTCATTCCCC (4667)
0.76
5831

osa-miR166f
TCGGACCAGGCTTCATTCCCC (4668)
0.81
5832

osa-miR166g
TCGGACCAGGCTTCATTCCTC (4669)
0.81
5833

osa-miR166h
TCGGACCAGGCTTCATTCCTC (4670)
0.81
5834

osa-miR166i
TCGGATCAGGCTTCATTCCTC (4671)
0.76
5835

osa-miR166j
TCGGATCAGGCTTCATTCCTC (4672)
0.76
5836

osa-miR166k
TCGGACCAGGCTTCAATCCCT (4673)
0.76
5837

osa-miR166l
TCGGACCAGGCTTCAATCCCT (4674)
0.76
5838

osa-
TCGGACCAGGCTTCATTCCCT (4675)
0.81
5839

miR166m

osa-miR166n
TCGGACCAGGCTTCATTCCCC (4676)
0.81
5840

pab-
TCGGACCAGGCTTCATTCCTC (4677)
0.81
5841

miR166a

pab-
TCGGACCAGGCTTCATTCCTT (4678)
0.81
5842

miR166b

pga-miR166
TCGGACCAGGCTTCATTCCTT (4679)
0.81
5843

ppt-miR166a
TCGGACCAGGCTTCATTCCCC (4680)
0.81
5844

ppt-miR166b
TCGGACCAGGCTTCATTCCCC (4681)
0.81
5845

ppt-miR166c
TCGGACCAGGCTTCATTCCCC (4682)
0.81
5846

ppt-miR166d
TCGGACCAGGCTTCATTCCCC (4683)
0.81
5847

ppt-miR166e
TCGGACCAGGCTTCATTCCCC (4684)
0.81
5848

ppt-miR166f
TCGGACCAGGCTTCATTCCCC (4685)
0.81
5849

ppt-miR166g
TCGGACCAGGCTTCATTCCCC (4686)
0.81
5850

ppt-miR166h
TCGGACCAGGCTTCATTCCCC (4687)
0.81
5851

ppt-miR166i
TCGGACCAGGCTTCATTCCCC (4688)
0.81
5852

ppt-miR166j
TCCGGACCAGGCTTCATTCCC (4689)
0.86
5853

ppt-miR166k
TCCGGACCAGGCTTCATTCCC (4690)
0.86
5854

ppt-miR166l
TCCGGACCAGGCTTCATTCCC (4691)
0.86
5855

ppt-
TCGGACCAGGCATCATTCCTT (4692)
0.76
5856

miR166m

pta-miR166a
TCGGACCAGGCTTCATTCCCC (4693)
0.81
5857

pta-miR166b
TCGGACCAGGCTTCATTCCCC (4694)
0.81
5858

ptc-miR166a
TCGGACCAGGCTTCATTCCCC (4695)
0.81
5859

ptc-miR166b
TCGGACCAGGCTTCATTCCCC (4696)
0.81
5860

ptc-miR166c
TCGGACCAGGCTTCATTCCCC (4697)
0.81
5861

ptc-miR166d
TCGGACCAGGCTTCATTCCCC (4698)
0.81
5862

ptc-miR166e
TCGGACCAGGCTTCATTCCCC (4699)
0.81
5863

ptc-miR166f
TCGGACCAGGCTTCATTCCCC (4700)
0.81
5864

ptc-miR166g
TCGGACCAGGCTTCATTCCCC (4701)
0.81
5865

ptc-miR166h
TCGGACCAGGCTTCATTCCCC (4702)
0.81
5866

ptc-miR166i
TCGGACCAGGCTTCATTCCCC (4703)
0.81
5867

ptc-miR166j
TCGGACCAGGCTTCATTCCCC (4704)
0.81
5868

ptc-miR166k
TCGGACCAGGCTTCATTCCCC (4705)
0.81
5869

ptc-miR166l
TCGGACCAGGCTTCATTCCCC (4706)
0.81
5870

ptc-
TCGGACCAGGCTTCATTCCCC (4707)
0.81
5871

miR166m

ptc-miR166n
TCGGACCAGGCTTCATTCCTT (4708)
0.81
5872

ptc-miR166o
TCGGACCAGGCTTCATTCCTT (4709)
0.81
5873

ptc-miR166p
TCGGACCAGGCTCCATTCCTT (4710)
0.76
5874

ptc-miR166q
TCGGACCAGGCTTCATTCCTT (4711)
0.81
5875

pvu-miR166
TCGGACCAGGCTTCATTCCCC (4712)
0.81
5876

pvu-
TCGGACCAGGCTTCATTCCCC (4713)
0.81
5877

miR166a

rco-miR166a
TCGGACCAGGCTTCATTCCCC (4714)
0.81
5878

rco-miR166b
TCGGACCAGGCTTCATTCCCC (4715)
0.81
5879

rco-miR166c
TCGGACCAGGCTTCATTCCCC (4716)
0.81
5880

rco-miR166d
TCGGACCAGGCTTCATTCCCC (4717)
0.81
5881

rco-miR166e
TCGGACCAGGCTTCATTCCCC (4718)
0.81
5882

sbi-miR166a
TCGGACCAGGCTTCATTCCC (4719)
0.81
5883

sbi-miR166b
TCGGACCAGGCTTCATTCCC (4720)
0.81
5884

sbi-miR166c
TCGGACCAGGCTTCATTCCC (4721)
0.81
5885

sbi-miR166d
TCGGACCAGGCTTCATTCCC (4722)
0.81
5886

sbi-miR166e
TCGGACCAGGCTTCAATCCCT (4723)
0.76
5887

sbi-miR166f
TCGGACCAGGCTTCATTCCTC (4724)
0.81
5888

sbi-miR166g
TCGGACCAGGCTTCAATCCCT (4725)
0.76
5889

sbi-miR166h
TCGGACCAGGCTTCATTCCC (4726)
0.81
5890

sbi-miR166i
TCGGACCAGGCTTCATTCCC (4727)
0.81
5891

sbi-miR166j
TCGGACCAGGCTTCATTCCC (4728)
0.81
5892

sbi-miR166k
TCGGACCAGGCTTCATTCCT (4729)
0.81
5893

sly-miR166a
TCGGACCAGGCTTCATTCCCC (4730)
0.81
5894

sly-miR166b
TCGGACCAGGCTTCATTCCCC (4731)
0.81
5895

smo-
TCGGACCAGGCTTCATTCCCC (4732)
0.81
5896

miR166a

smo-
TCGGACCAGGCTTCATTCCCC (4733)
0.81
5897

miR166b

smo-
TCGGACCAGGCTTCATTCCCC (4734)
0.81
5898

miR166c

sof-miR166
TCGGACCAGGCTTCATTCCCC (4735)
0.81
5899

tae-miR166
CCGGACCAGGCTTCATTCCCA (4736)
0.86
5900

tcc-miR166a
TCGGACCAGGCTTCATTCCCC (4737)
0.81
5901

tcc-miR166b
TCGGACCAGGCTTCATTCCC (4738)
0.81
5902

tcc-miR166c
TCGGACCAGGCTTCATTCCTC (4739)
0.81
5903

tcc-miR166d
TCGGACCAGGCTTCATTCCCC (4740)
0.81
5904

vvi-miR166a
TCGGACCAGGCTTCATTCC (4741)
0.81
5905

vvi-miR166b
TCGGACCAGGCTTCATTCC (4742)
0.81
5906

vvi-miR166c
TCGGACCAGGCTTCATTCCCC (4743)
0.81
5907

vvi-miR166d
TCGGACCAGGCTTCATTCCCC (4744)
0.81
5908

vvi-miR166e
TCGGACCAGGCTTCATTCCCC (4745)
0.81
5909

vvi-miR166f
TCGGACCAGGCTTCATTCCCC (4746)
0.81
5910

vvi-miR166g
TCGGACCAGGCTTCATTCCCC (4747)
0.81
5911

vvi-miR166h
TCGGACCAGGCTTCATTCCCC (4748)
0.81
5912

zma-
TCGGACCAGGCTTCATTCCCC (4749)
0.81
5913

miR166a

zma-
TCGGACCAGGCTTCATTCCC (4750)
0.81
5914

miR166b

zma-
TCGGACCAGGCTTCATTCCC (4751)
0.81
5915

miR166c

zma-
TCGGACCAGGCTTCATTCCC (4752)
0.81
5916

miR166d

zma-
TCGGACCAGGCTTCATTCCC (4753)
0.81
5917

miR166e

zma-
TCGGACCAGGCTTCATTCCC (4754)
0.81
5918

miR166f

zma-
TCGGACCAGGCTTCATTCCC (4755)
0.81
5919

miR166g

zma-
TCGGACCAGGCTTCATTCCC (4756)
0.81
5920

miR166h

zma-
TCGGACCAGGCTTCATTCCC (4757)
0.81
5921

miR166i

zma-
TCGGACCAGGCTTCAATCCCT (4758)
0.76
5922

miR166j

zma-
TCGGACCAGGCTTCAATCCCT (4759)
0.76
5923

miR166k

zma-
TCGGACCAGGCTTCATTCCTC (4760)
0.81
5924

miR166l

zma-
TCGGACCAGGCTTCATTCCTC (4761)
0.81
5925

miR166m

zma-
TCGGACCAGGCTTCAATCCCT (4762)
0.76
5926

miR166n

zma-
TCGGACCAGGCTTCATTCCCC (4763)
0.81
5927

miR166o

zma-
TCGGACCAGGCTTCATTCCCC (4764)
0.81
5928

miR166p

zma-
TCGGACCAGGCTTCATTCCCC (4765)
0.81
5929

miR166q

zma-
TCGGACCAGGCTTCATTCCCC (4766)
0.81
5930

miR166r

zma-
TCGGACCAGGCTTCATTCCCC (4767)
0.81
5931

miR166s

zma-
TCGGACCAGGCTTCATTCCCC (4768)
0.81
5932

miR166t

zma-
TCGGACCACGCTTCATTCCCC (4769)
0.76
5933

miR166u

ptc-miR166p
aly-miR166a
TCGGACCAGGCTTCATTCCCC (4770)
0.86
5934

aly-miR166b
TCGGACCAGGCTTCATTCCCC (4771)
0.86
5935

aly-miR166c
TCGGACCAGGCTTCATTCCCC (4772)
0.86
5936

aly-miR166d
TCGGACCAGGCTTCATTCCCC (4773)
0.86
5937

aly-miR166e
TCGGACCAGGCTTCATTCCCC (4774)
0.86
5938

aly-miR166f
TCGGACCAGGCTTCATTCCCC (4775)
0.86
5939

aly-miR166g
TCGGACCAGGCTTCATTCCCC (4776)
0.86
5940

aqc-miR166a
TCGGACCAGGCTTCATTCCTC (4777)
0.9
5941

aqc-
TCGGACCAGGCTTCATTCCCC (4778)
0.86
5942

miR166b

aqc-miR166c
TCGGACCAGGCTTCATTCCT (4779)
0.9
5943

aqc-
TCGGACCAGGCTTCATTCCTC (4780)
0.9
5944

miR166d

aqc-miR166e
TCGGACCAGGCTTCATTCCCC (4781)
0.86
5945

ath-miR166a
TCGGACCAGGCTTCATTCCCC (4782)
0.86
5946

ath-miR166b
TCGGACCAGGCTTCATTCCCC (4783)
0.86
5947

ath-miR166c
TCGGACCAGGCTTCATTCCCC (4784)
0.86
5948

ath-miR166d
TCGGACCAGGCTTCATTCCCC (4785)
0.86
5949

ath-miR166e
TCGGACCAGGCTTCATTCCCC (4786)
0.86
5950

ath-miR166f
TCGGACCAGGCTTCATTCCCC (4787)
0.86
5951

ath-miR166g
TCGGACCAGGCTTCATTCCCC (4788)
0.86
5952

bdi-miR166
TCGGACCAGGCTTCATTCCCC (4789)
0.86
5953

bdi-miR166a
TCGGACCAGGCTTCATTCCCC (4790)
0.86
5954

bdi-miR166b
TCGGACCAGGCTTCATTCCCC (4791)
0.86
5955

bdi-miR166c
TCGGACCAGGCTTCATTCCCC (4792)
0.86
5956

bdi-miR166d
TCGGACCAGGCTTCATTCCCC (4793)
0.86
5957

bdi-miR166e
CTCGGACCAGGCTTCATTCCC (4794)
0.86
5958

bdi-miR166f
TCTCGGACCAGGCTTCATTCC (4795)
0.86
5959

bna-
TCGGACCAGGCTTCATTCCCC (4796)
0.86
5960

miR166a

bna-
TCGGACCAGGCTTCATTCCCC (4797)
0.86
5961

miR166b

bna-
TCGGACCAGGCTTCATTCCCC (4798)
0.86
5962

miR166c

bna-
TCGGACCAGGCTTCATTCCCC (4799)
0.86
5963

miR166d

cpt-miR166
TCGGACCAGGCTTCATTCCC (4800)
0.86
5964

crt-miR166a
TCGGACCAGGCTTCATTCCCGT (4801)
0.86
5965

crt-miR166b
TCGGACCAGGCTTCATTCCCTT (4802)
0.9
5966

csi-miR166
TCGGACCAGGCTTCATTCCCC (4803)
0.86
5967

csi-miR166a
TCGGACCAGGCTTCATTCCCCC (4804)
0.86
5968

csi-miR166b
TCGGACCAGGCTTCATTCCCGT (4805)
0.86
5969

csi-miR166c
TCGGACCAGGCTTCATTCCC (4806)
0.86
5970

csi-miR166d
TCGGACCAGGCTTCATTCCCT (4807)
0.9
5971

csi-miR166e
TCGGACCAGGCTTCATTCCCC (4808)
0.86
5972

ctr-miR166
TCGGACCAGGCTTCATTCCCCC (4809)
0.86
5973

far-miR166
CCGGACCAGGCTTCATCCCAG (4810)
0.76
5974

flm-miR166
TCGGACCAGGCTTCATCCCCC (4811)
0.81
5975

ghr-miR166a
TCGGACCAGGCTTCATTCCCC (4812)
0.86
5976

ghr-miR166b
TCGGACCAGGCTTCATTCCCC (4813)
0.86
5977

gma-
TCGGACCAGGCTTCATTCCCC (4814)
0.86
5978

miR166a

gma-
TCGGACCAGGCTTCATTCCCC (4815)
0.86
5979

miR166b

gma-
TCGGACCAGGCTTCATTCCCC (4816)
0.86
5980

miR166n

gma-
TCGGACCAGGCTTCATTCCCC (4817)
0.86
5981

miR166o

gma-
TCGGACCAGGCTTCATTCCCG (4818)
0.86
5982

miR166q

gma-
TCGGACCAGGCTTCATTCCCT (4819)
0.9
5983

miR166r

hvu-miR166
TCGGACCAGGCTTCATTCCCC (4820)
0.86
5984

hvu-
TCGGACCAGGCTTCATTCCCC (4821)
0.86
5985

miR166b

hvu-
TCGGACCAGGCTTCATTCCCC (4822)
0.86
5986

miR166c

hvv-miR166
TCGGACCAGGCTTCATTCCCC (4823)
0.86
5987

ini-miR166
TCGGACCAGGCTTCATTCCTC (4824)
0.9
5988

mtr-miR166
TCGGACCAGGCTTCATTCCCC (4825)
0.86
5989

mtr-
TCGGACCAGGCTTCATTCCTA (4826)
0.9
5990

miR166b
(TCGGACCAGGCTTCATTCCCC (5116)

mtr-miR166c
TCGGACCAGGCTTCATTCCTC (4827)
0.9
5991

mtr-
TCGGGCCAGGCTTCATCCCCC (4828)
0.76
5992

miR166d

mtr-miR166e
TCGGACCAGGCTTCATTCCCC (4829)
0.86
5993

mtr-miR166f
TCGGACCAGGCTTCATTCCTC (4830)
0.9
5994

mtr-
TCGGACCAGGCTTCATTCCCC (4831)
0.86
5995

miR166g

mtr-
TCGGACCAGGCTTCATTCCCC (4832)
0.86
5996

miR166h

nsy-miR166
TCGGACCAGGCTTCATTCCCC (4833)
0.86
5997

osa-miR166a
TCGGACCAGGCTTCATTCCCC (4834)
0.86
5998

osa-miR166b
TCGGACCAGGCTTCATTCCCC (4835)
0.86
5999

osa-miR166c
TCGGACCAGGCTTCATTCCCC (4836)
0.86
6000

osa-miR166d
TCGGACCAGGCTTCATTCCCC (4837)
0.86
6001

osa-miR166e
TCGAACCAGGCTTCATTCCCC (4838)
0.81
6002

osa-miR166f
TCGGACCAGGCTTCATTCCCC (4839)
0.86
6003

osa-miR166g
TCGGACCAGGCTTCATTCCTC (4840)
0.9
6004

osa-miR166h
TCGGACCAGGCTTCATTCCTC (4841)
0.9
6005

osa-miR166i
TCGGATCAGGCTTCATTCCTC (4842)
0.86
6006

osa-miR166j
TCGGATCAGGCTTCATTCCTC (4843)
0.86
6007

osa-miR166k
TCGGACCAGGCTTCAATCCCT (4844)
0.86
6008

osa-miR166l
TCGGACCAGGCTTCAATCCCT (4845)
0.86
6009

osa-
TCGGACCAGGCTTCATTCCCT (4846)
0.9
6010

miR166m

osa-miR166n
TCGGACCAGGCTTCATTCCCC (4847)
0.86
6011

pab-
TCGGACCAGGCTTCATTCCTC (4848)
0.9
6012

miR166a

pab-
TCGGACCAGGCTTCATTCCTT (4849)
0.95
6013

miR166b

pga-miR166
TCGGACCAGGCTTCATTCCTT (4850)
0.95
6014

ppt-miR166a
TCGGACCAGGCTTCATTCCCC (4851)
0.86
6015

ppt-miR166b
TCGGACCAGGCTTCATTCCCC (4852)
0.86
6016

ppt-miR166c
TCGGACCAGGCTTCATTCCCC (4853)
0.86
6017

ppt-miR166d
TCGGACCAGGCTTCATTCCCC (4854)
0.86
6018

ppt-miR166e
TCGGACCAGGCTTCATTCCCC (4855)
0.86
6019

ppt-miR166f
TCGGACCAGGCTTCATTCCCC (4856)
0.86
6020

ppt-miR166g
TCGGACCAGGCTTCATTCCCC (4857)
0.86
6021

ppt-miR166h
TCGGACCAGGCTTCATTCCCC (4858)
0.86
6022

ppt-miR166i
TCGGACCAGGCTTCATTCCCC (4859)
0.86
6023

ppt-miR166j
TCCGGACCAGGCTTCATTCCC (4860)
0.81
6024

ppt-miR166k
TCCGGACCAGGCTTCATTCCC (4861)
0.81
6025

ppt-miR166l
TCCGGACCAGGCTTCATTCCC (4862)
0.81
6026

ppt-
TCGGACCAGGCATCATTCCTT (4863)
0.9
6027

miR166m

pta-miR166a
TCGGACCAGGCTTCATTCCCC (4864)
0.86
6028

pta-miR166b
TCGGACCAGGCTTCATTCCCC (4865)
0.86
6029

pta-miR166c
CCGGACCAGGCTTCATCCCAG (4866)
0.76
6030

ptc-miR166a
TCGGACCAGGCTTCATTCCCC (4867)
0.86
6031

ptc-miR166b
TCGGACCAGGCTTCATTCCCC (4868)
0.86
6032

ptc-miR166c
TCGGACCAGGCTTCATTCCCC (4869)
0.86
6033

ptc-miR166d
TCGGACCAGGCTTCATTCCCC (4870)
0.86
6034

ptc-miR166e
TCGGACCAGGCTTCATTCCCC (4871)
0.86
6035

ptc-miR166f
TCGGACCAGGCTTCATTCCCC (4872)
0.86
6036

ptc-miR166g
TCGGACCAGGCTTCATTCCCC (4873)
0.86
6037

ptc-miR166h
TCGGACCAGGCTTCATTCCCC (4874)
0.86
6038

ptc-miR166i
TCGGACCAGGCTTCATTCCCC (4875)
0.86
6039

ptc-miR166j
TCGGACCAGGCTTCATTCCCC (4876)
0.86
6040

ptc-miR166k
TCGGACCAGGCTTCATTCCCC (4877)
0.86
6041

ptc-miR166l
TCGGACCAGGCTTCATTCCCC (4878)
0.86
6042

ptc-
TCGGACCAGGCTTCATTCCCC (4879)
0.86
6043

miR166m

ptc-miR166n
TCGGACCAGGCTTCATTCCTT (4880)
0.95
6044

ptc-miR166o
TCGGACCAGGCTTCATTCCTT (4881)
0.95
6045

ptc-miR166q
TCGGACCAGGCTTCATTCCTT (4882)
0.95
6046

pvu-miR166
TCGGACCAGGCTTCATTCCCC (4883)
0.86
6047

pvu-
TCGGACCAGGCTTCATTCCCC (4884)
0.86
6048

miR166a

rco-miR166a
TCGGACCAGGCTTCATTCCCC (4885)
0.86
6049

rco-miR166b
TCGGACCAGGCTTCATTCCCC (4886)
0.86
6050

rco-miR166c
TCGGACCAGGCTTCATTCCCC (4887)
0.86
6051

rco-miR166d
TCGGACCAGGCTTCATTCCCC (4888)
0.86
6052

rco-miR166e
TCGGACCAGGCTTCATTCCCC (4889)
0.86
6053

sbi-miR166a
TCGGACCAGGCTTCATTCCC (4890)
0.86
6054

sbi-miR166b
TCGGACCAGGCTTCATTCCC (4891)
0.86
6055

sbi-miR166c
TCGGACCAGGCTTCATTCCC (4892)
0.86
6056

sbi-miR166d
TCGGACCAGGCTTCATTCCC (4893)
0.86
6057

sbi-miR166e
TCGGACCAGGCTTCAATCCCT (4894)
0.86
6058

sbi-miR166f
TCGGACCAGGCTTCATTCCTC (4895)
0.9
6059

sbi-miR166g
TCGGACCAGGCTTCAATCCCT (4896)
0.86
6060

sbi-miR166h
TCGGACCAGGCTTCATTCCC (4897)
0.86
6061

sbi-miR166i
TCGGACCAGGCTTCATTCCC (4898)
0.86
6062

sbi-miR166j
TCGGACCAGGCTTCATTCCC (4899)
0.86
6063

sbi-miR166k
TCGGACCAGGCTTCATTCCT (4900)
0.9
6064

sly-miR166a
TCGGACCAGGCTTCATTCCCC (4901)
0.86
6065

sly-miR166b
TCGGACCAGGCTTCATTCCCC (4902)
0.86
6066

smo-
TCGGACCAGGCTTCATTCCCC (4903)
0.86
6067

miR166a

smo-
TCGGACCAGGCTTCATTCCCC (4904)
0.86
6068

miR166b

smo-
TCGGACCAGGCTTCATTCCCC (4905)
0.86
6069

miR166c

sof-miR166
TCGGACCAGGCTTCATTCCCC (4906)
0.86
6070

tae-miR166
CCGGACCAGGCTTCATTCCCA (4907)
0.81
6071

tcc-miR166a
TCGGACCAGGCTTCATTCCCC (4908)
0.86
6072

tcc-miR166b
TCGGACCAGGCTTCATTCCC (4909)
0.86
6073

tcc-miR166c
TCGGACCAGGCTTCATTCCTC (4910)
0.9
6074

tcc-miR166d
TCGGACCAGGCTTCATTCCCC (4911)
0.86
6075

vvi-miR166a
TCGGACCAGGCTTCATTCC (4912)
0.86
6076

vvi-miR166b
TCGGACCAGGCTTCATTCC (4913)
0.86
6077

vvi-miR166c
TCGGACCAGGCTTCATTCCCC (4914)
0.86
6078

vvi-miR166d
TCGGACCAGGCTTCATTCCCC (4915)
0.86
6079

vvi-miR166e
TCGGACCAGGCTTCATTCCCC (4916)
0.86
6080

vvi-miR166f
TCGGACCAGGCTTCATTCCCC (4917)
0.86
6081

vvi-miR166g
TCGGACCAGGCTTCATTCCCC (4918)
0.86
6082

vvi-miR166h
TCGGACCAGGCTTCATTCCCC (4919)
0.86
6083

zma-
TCGGACCAGGCTTCATTCCCC (4920)
0.86
6084

miR166a

zma-
TCGGACCAGGCTTCATTCCC (4921)
0.86
6085

miR166b

zma-
TCGGACCAGGCTTCATTCCC (4922)
0.86
6086

miR166c

zma-
TCGGACCAGGCTTCATTCCC (4923)
0.86
6087

miR166d

zma-
TCGGACCAGGCTTCATTCCC (4924)
0.86
6088

miR166e

zma-
TCGGACCAGGCTTCATTCCC (4925)
0.86
6089

miR166f

zma-
TCGGACCAGGCTTCATTCCC (4926)
0.86
6090

miR166g

zma-
TCGGACCAGGCTTCATTCCC (4927)
0.86
6091

miR166h

zma-
TCGGACCAGGCTTCATTCCC (4928)
0.86
6092

miR166i

zma-
TCGGACCAGGCTTCAATCCCT (4929)
0.86
6093

miR166j

zma-
TCGGACCAGGCTTCAATCCCT (4930)
0.86
6094

miR166k

zma-
TCGGACCAGGCTTCATTCCTC (4931)
0.9
6095

miR166l

zma-
TCGGACCAGGCTTCATTCCTC (4932)
0.9
6096

miR166m

zma-
TCGGACCAGGCTTCAATCCCT (4933)
0.86
6097

miR166n

zma-
TCGGACCAGGCTTCATTCCCC (4934)
0.86
6098

miR166o

zma-
TCGGACCAGGCTTCATTCCCC (4935)
0.86
6099

miR166p

zma-
TCGGACCAGGCTTCATTCCCC (4936)
0.86
6100

miR166q

zma-
TCGGACCAGGCTTCATTCCCC (4937)
0.86
6101

miR166r

zma-
TCGGACCAGGCTTCATTCCCC (4938)
0.86
6102

miR166s

zma-
TCGGACCAGGCTTCATTCCCC (4939)
0.86
6103

miR166t

zma-
TCGGACCACGCTTCATTCCCC (4940)
0.81
6104

miR166u

vvi-miR394b
ahy-miR394
TTGGCATTCTGTCCACCTCC (4941)
1
6105

aly-miR394a
TTGGCATTCTGTCCACCTCC (4942)
1
6106

aly-miR394b
TTGGCATTCTGTCCACCTCC (4943)
1
6107

ath-miR394a
TTGGCATTCTGTCCACCTCC (4944)
1
6108

ath-miR394b
TTGGCATTCTGTCCACCTCC (4945)
1
6109

bdi-miR394
TTGGCATTCTGTCCACCTCC (4946)
1
6110

csi-miR394
TTGGCATTCTGTCCACCTCC (4947)
1
6111

ghr-miR394
TTGGCATTCTGTCCACCTCC (4948)
1
6112

ghr-miR394a
TTGGCATTCTGTCCACCTCC (4949)
1
6113

ghr-miR394b
TTGGCATTCTGTCCACCTCC (4950)
1
6114

gma-
AGGTGGGCATACTGTCAACT (4951)
0.65
6115

miR394b

osa-miR394
TTGGCATTCTGTCCACCTCC (4952)
1
6116

ptc-
TTGGCATTCTGTCCACCTCC (4953)
1
6117

miR394a-5p

ptc-
TTGGCATTCTGTCCACCTCC (4954)
1
6118

miR394b-5p

sbi-miR394a
TTGGCATTCTGTCCACCTCC (4955)
1
6119

sbi-miR394b
TTGGCATTCTGTCCACCTCC (4956)
1
6120

tcc-miR394a
TTGGCATTCTGTCCACCTCC (4957)
1
6121

tcc-miR394b
TTGGCATTCTGTCCACCTCC (4958)
1
6122

vvi-miR394a
TTGGCATTCTGTCCACCTCCAT (4959)
1
6123

vvi-miR394c
TTGGCATTCTGTCCACCTCCAT (4960)
1
6124

zma-
TTGGCATTCTGTCCACCTCC (4961)
1
6125

miR394a

zma-
TTGGCATTCTGTCCACCTCC (4962)
1
6126

miR394b

zma-miR167u
ahy-miR167-
TGAAGCTGCCAGCATGATCTT (4963)
0.95
6127

5p

aly-miR167a
TGAAGCTGCCAGCATGATCTA (4964)
0.95
6128

aly-miR167b
TGAAGCTGCCAGCATGATCTA (4965)
0.95
6129

aly-
GGTCATGCTCTGACAGCCTCACT (4966)
0.5
6130

miR167b*

aly-miR167c
TAAGCTGCCAGCATGATCTTG (4967)
0.85
6131

aly-miR167d
TGAAGCTGCCAGCATGATCTGG (4968)
1
6132

aqc-miR167
TCAAGCTGCCAGCATGATCTA (4969)
0.9
6133

ath-miR167a
TGAAGCTGCCAGCATGATCTA (4970)
0.95
6134

ath-miR167b
TGAAGCTGCCAGCATGATCTA (4971)
0.95
6135

ath-miR167c
TAAGCTGCCAGCATGATCTTG (4972)
0.85
6136

ath-miR167d
TGAAGCTGCCAGCATGATCTGG (4973)
1
6137

ath-
TGAAGCTGCCAGCATGATCTG (4974)
1
6138

miR167m

bdi-miR167
TGAAGCTGCCAGCATGATCTA (4975)
0.95
6139

bdi-miR167a
TGAAGCTGCCAGCATGATCTA (4976)
0.95
6140

bdi-miR167b
TGAAGCTGCCAGCATGATCTA (4977)
0.95
6141

bdi-miR167c
TGAAGCTGCCAGCATGATCTGA (4978)
1
6142

bdi-miR167d
TGAAGCTGCCAGCATGATCTGA (4979)
1
6143

bna-
TGAAGCTGCCAGCATGATCTAA (4980)
0.95
6144

miR167a

bna-
TGAAGCTGCCAGCATGATCTAA (4981)
0.95
6145

miR167b

bna-
TGAAGCTGCCAGCATGATCTA (4982)
0.95
6146

miR167c

bra-miR167a
TGAAGCTGCCAGCATGATCTA (4983)
0.95
6147

bra-miR167b
TGAAGCTGCCAGCATGATCTA (4984)
0.95
6148

bra-miR167c
TGAAGCTGCCAGCATGATCTA (4985)
0.95
6149

bra-miR167d
TGAAGCTGCCAGCATGATCTA (4986)
0.95
6150

ccl-miR167a
TGAAGCTGCCAGCATGATCTGA (4987)
1
6151

ccl-miR167b
TGAAGCTGCCAGCATGATCTGA (4988)
1
6152

cle-miR167
TGAAGCTGCCAGCATGATCTG (4989)
1
6153

csi-miR167a
TGAAGCTGCCAGCATGATCTG (4990)
1
6154

csi-miR167b
TGAAGCTGCCAGCATGATCTT (4991)
0.95
6155

csi-miR167c
TGAAGCTGCCAGCATGATCTG (4992)
1
6156

ctr-miR167
TGAAGCTGCCAGCATGATCTGA (4993)
1
6157

ghr-miR167
TGAAGCTGCCAGCATGATCTA (4994)
0.95
6158

gma-
TGAAGCTGCCAGCATGATCTA (4995)
0.95
6159

miR167a

gma-
TGAAGCTGCCAGCATGATCTA (4996)
0.95
6160

miR167b

gma-
TGAAGCTGCCAGCATGATCTG (4997)
1
6161

miR167c

gma-
TGAAGCTGCCAGCATGATCTA (4998)
0.95
6162

miR167d

gma-
TGAAGCTGCCAGCATGATCTT (4999)
0.95
6163

miR167e

gma-
TGAAGCTGCCAGCATGATCTT (5000)
0.95
6164

miR167f

gma-
TGAAGCTGCCAGCATGATCTGA (5001)
1
6165

miR167g

gma-
TGAAGCTGCCAGCATGATCT (5002)
0.95
6166

miR167n

gma-
TGAAGCTGCCAGCATGATCTG (5003)
1
6167

miR167o

gso-miR167a
TGAAGCTGCCAGCATGATCTG (5004)
1
6168

ini-miR167
TGAAGCTGCCAGCATGATCTG (5005)
1
6169

lja-miR167
TGAAGCTGCCAGCATGATCTG (5006)
1
6170

mtr-miR167
TGAAGCTGCCAGCATGATCTA (5007)
0.95
6171

osa-miR167a
TGAAGCTGCCAGCATGATCTA (5008)
0.95
6172

osa-
ATCATGCATGACAGCCTCATTT (5009)
0.65
6173

miR167a*

osa-miR167b
TGAAGCTGCCAGCATGATCTA (5010)
0.95
6174

osa-miR167c
TGAAGCTGCCAGCATGATCTA (5011)
0.95
6175

osa-miR167d
TGAAGCTGCCAGCATGATCTG (5012)
1
6176

osa-miR167e
TGAAGCTGCCAGCATGATCTG (5013)
1
6177

osa-miR167f
TGAAGCTGCCAGCATGATCTG (5014)
1
6178

osa-miR167g
TGAAGCTGCCAGCATGATCTG (5015)
1
6179

osa-miR167h
TGAAGCTGCCAGCATGATCTG (5016)
1
6180

osa-miR167i
TGAAGCTGCCAGCATGATCTG (5017)
1
6181

osa-miR167j
TGAAGCTGCCAGCATGATCTG (5018)
1
6182

osa-
TGAAGCTGCCAGCATGATCTG (5019)
1
6183

miR167m

osa-miR167n
TGAAGCTGCCAGCATGATCTG (5020)
1
6184

pco-miR167
TGAAGCTGCCAGCATGATCTT (5021)
0.95
6185

ppl-miR167a
TGAAGCTGCCAGCATGATCTA (5022)
0.95
6186

ppl-miR167b
TGAAGCTGCCAGCATGATCTG (5023)
1
6187

ppt-miR167
GGAAGCTGCCAGCATGATCCT (5024)
0.85
6188

ptc-miR167a
TGAAGCTGCCAGCATGATCTA (5025)
0.95
6189

ptc-miR167b
TGAAGCTGCCAGCATGATCTA (5026)
0.95
6190

ptc-miR167c
TGAAGCTGCCAGCATGATCTA (5027)
0.95
6191

ptc-miR167d
TGAAGCTGCCAGCATGATCTA (5028)
0.95
6192

ptc-miR167e
TGAAGCTGCCAGCATGATCTG (5029)
1
6193

ptc-miR167f
TGAAGCTGCCAGCATGATCTT (5030)
0.95
6194

ptc-miR167g
TGAAGCTGCCAGCATGATCTT (5031)
0.95
6195

ptc-miR167h
TGAAGCTGCCAACATGATCTG (5032)
1
6196

pts-miR167
TGAAGCTGCCAGCATGATCTG (5033)
1
6197

rco-miR167a
TGAAGCTGCCAGCATGATCTA (5034)
0.95
6198

rco-miR167b
TGAAGCTGCCAGCATGATCTA (5035)
0.95
6199

rco-miR167c
TGAAGCTGCCAGCATGATCTGG (5036)
1
6200

sbi-miR167a
TGAAGCTGCCAGCATGATCTA (5037)
0.95
6201

sbi-miR167b
TGAAGCTGCCAGCATGATCTA (5038)
0.95
6202

sbi-miR167c
TGAAGCTGCCAGCATGATCTG (5039)
1
6203

sbi-miR167d
TGAAGCTGCCAGCATGATCTG (5040)
1
6204

sbi-miR167e
TGAAGCTGCCAGCATGATCTG (5041)
1
6205

sbi-miR167f
TGAAGCTGCCAGCATGATCTG (5042)
1
6206

sbi-miR167g
TGAAGCTGCCAGCATGATCTG (5043)
1
6207

sbi-miR167h
TGAAGCTGCCAGCATGATCTG (5044)
1
6208

sbi-miR167i
TGAAGCTGCCAGCATGATCTA (5045)
0.95
6209

sly-miR167
TGAAGCTGCCAGCATGATCTA (5046)
0.95
6210

sof-miR167a
TGAAGCTGCCAGCATGATCTG (5047)
1
6211

sof-miR167b
TGAAGCTGCCAGCATGATCTG (5048)
1
6212

ssp-miR167
TGAAGCTGCCAGCATGATCTG (5049)
1
6213

ssp-miR167b
TGAAGCTGCCAGCATGATCTG (5050)
1
6214

tae-miR167
TGAAGCTGCCAGCATGATCTA (5051)
0.95
6215

tae-miR167b
TGAAGCTGACAGCATGATCTA (5052)
0.9
6216

tcc-miR167a
TGAAGCTGCCAGCATGATCTA (5053)
0.95
6217

tcc-miR167b
TGAAGCTGCCAGCATGATCTA (5054)
0.95
6218

tcc-miR167c
TGAAGCTGCCAGCATGATCTT (5055)
0.95
6219

vvi-miR167a
TGAAGCTGCCAGCATGATCTG (5056)
1
6220

vvi-miR167b
TGAAGCTGCCAGCATGATCTA (5057)
0.95
6221

vvi-miR167c
TGAAGCTGCCAGCATGATCTC (5058)
0.95
6222

vvi-miR167d
TGAAGCTGCCAGCATGATCTA (5059)
0.95
6223

vvi-miR167e
TGAAGCTGCCAGCATGATCTA (5060)
0.95
6224

zma-
TGAAGCTGCCAGCATGATCTA (5061)
0.95
6225

miR167a

zma-
TGAAGCTGCCAGCATGATCTA (5062)
0.95
6226

miR167b

zma-
GATCATGCTGTGACAGTTTCACT (5063)
0.55
6227

miR167b*

zma-
TGAAGCTGCCAGCATGATCTA (5064)
0.95
6228

miR167c

zma-
TGAAGCTGCCAGCATGATCTA (5065)
0.95
6229

miR167d

zma-
TGAAGCTGCCAGCATGATCTG (5066)
1
6230

miR167e

zma-
TGAAGCTGCCAGCATGATCTG (5067)
1
6231

miR167f

zma-
TGAAGCTGCCAGCATGATCTG (5068)
1
6232

miR167g

zma-
TGAAGCTGCCAGCATGATCTG (5069)
1
6233

miR167h

zma-
TGAAGCTGCCAGCATGATCTG (5070)
1
6234

miR167i

zma-
TGAAGCTGCCAGCATGATCTG (5071)
1
6235

miR167j

zma-
TGAAGCTGCCAGCATGATCTG (5072)
1
6236

miR167k

zma-
TGAAGCTGCCAGCATGATCTG (5073)
1
6237

miR167l

zma-
TGAAGCTGCCAGCATGATCTG (5074)
1
6238

miR167m

zma-
TGAAGCTGCCAGCATGATCTA (5075)
0.95
6239

miR167n

zma-
TGAAGCTGCCAGCATGATCTA (5076)
0.95
6240

miR167o

zma-
TGAAGCTGCCAGCATGATCTA (5077)
0.95
6241

miR167p

zma-
TGAAGCTGCCAGCATGATCTA (5078)
0.95
6242

miR167q

zma-
TGAAGCTGCCAGCATGATCTA (5079)
0.95
6243

miR167r

zma-
TGAAGCTGCCAGCATGATCTA (5080)
0.95
6244

miR167s

zma-
TGAAGCTGCCAGCATGATCTA (5081)
0.95
6245

miR167t

gma-miR2119
mtr-
TCAAAGGGAGGTGTGGAGTAG (5082)
0.76
6246

miR2119

pvu-
TCAAAGGGAGTTGTAGGGGAA (5083)
1
6247

miR2119

osa-miR162a
aly-miR162a
TCGATAAACCTCTGCATCCAG (5084)
1
6248

aly-miR162b
TCGATAAACCTCTGCATCCAG (5085)
1
6249

ath-miR162a
TCGATAAACCTCTGCATCCAG (5086)
1
6250

ath-miR162b
TCGATAAACCTCTGCATCCAG (5087)
1
6251

bdi-miR162
TCGATAAACCTCTGCATCCGG (5088)
0.95
6252

cpa-miR162a
TCGATAAACCTCTGCATCCAG (5089)
1
6253

csi-miR162
TCGATAAACCTCTGCATCCAG (5090)
1
6254

csi-miR162.5
TCGATAAACCTCTGCATCCAG (5091)
1
6255

ghr-miR162a
TCGATAAACCTCTGCATCCAG (5092)
1
6256

gma-miR162
TCGATAAACCTCTGCATCCA (5093)
0.95
6257

gma-
TCGATAAACCTCTGCATCCAG (5094)
1
6258

miR162a

gma-
TCGATAAACCTCTGCATCCAG (5095)
1
6259

miR162m

llu-miR162
TCGATAAACCTCTGCATCCAG (5096)
1
6260

lsa-miR162
TCGATAAACCTCTGCATCCAG (5097)
1
6261

mdo-miR162
TCGATAAACCTTTGCATCCAG (5098)
0.95
6262

mtr-miR162
TCGATAAACCTCTGCATCCAG (5099)
1
6263

mtr-
TCGATAAACCTCTGCATCCA (5100)
0.95
6264

miR162b

mtr-miR162c
TCGATGAACCGCTGCATCCAG (5101)
0.9
6265

mtr-
TCGATAAACCTCTGCATCCAG (5102)
1
6266

miR162d

osa-miR162b
TCGATAAGCCTCTGCATCCAG (5103)
0.95
6267

osa-
TCGATAAGCCTCTGCATCCAG (5104)
0.95
6268

miR162m

ptc-miR162a
TCGATAAACCTCTGCATCCAG (5105)
1
6269

ptc-miR162b
TCGATAAACCTCTGCATCCAG (5106)
1
6270

ptc-miR162c
TCGATAAACCTCTGCATCCAG (5107)
1
6271

rco-miR162
TCGATAAACCTCTGCATCCAG (5108)
1
6272

sbi-miR162
TCGATAAACCTCTGCATCCAG (5109)
1
6273

sly-miR162
TCGATAAACCTCTGCATCCAG (5110)
1
6274

tcc-miR162
TCGATAAACCTCTGCATCCAG (5111)
1
6275

vvi-miR162
TCGATAAACCTCTGCATCCAG (5112)
1
6276

zma-miR162
TCGATAAACCTCTGCATCCA (5113)
0.95
6277

zma-
TCGATAAACCTCTGCATCCAG (5114)
1
6278

miR162b

Example 3
Identification of miRNAs Associated with Abiotic Stress and Target Prediction Using Bioinformatics Tools

Small RNAs that are potentially associated with improved abiotic or biotic stress tolerance can be identified by proprietary computational algorithms that analyze RNA expression profiles alongside publicly available gene and protein databases. A high throughput screening is performed on microarrays loaded with miRNAs that were found to be differential under multiple stress and optimal environmental conditions and in different plant tissues. The initial trait-associated miRNAs are later validated by quantitative Real Time PCR (qRT-PCR).

Target prediction—homologous or orthologous genes to the genes of interest in soybean and/or arabidopsis are found through a proprietary tool that analyzes publicly available genomic as well as expression and gene annotation databases from multiple plant species. Homologous and orthologous protein and nucleotide sequences of target genes of the small RNA sequences of the invention, were found using BLAST having at least 70% identity on at least 60% of the entire master gene length, and are summarized in Tables 9-10 below.

TABLE 9

Target Genes of upregulated Small RNA Molecules Associated with Abiotic Stress Tolerance in Soybean Plants.

Mir
Homolog
Nucleotide

Binding
NCBI
NCBI GI

Protein
Nucleotide

Mir Name
Position
Accession
number
Identity
Organism
Seq id no:
Seq id no:

aqc-
305-325
XP_003551790
356567161
1

Glycine max

6315
8130

miR159
334-354
XP_003554567
356572827
1

Glycine max

6316
8131

XP_003521605
356505654
0.9723

Glycine max

6317
8132

XP_003521606
356505656
0.9723

Glycine max

6318
8133

XP_003626013
357511448
0.8949

Medicago truncatula

6319
8134

XP_002272575
225437676
0.8423

Vitis vinifera

6320
8135

CBI37003
270254427
0.8409

Vitis vinifera

6321
8136

XP_002515224
255761086
0.8382

Ricinus communis

6322

CAN71135
147786943
0.8354

Vitis vinifera

6323
8137

XP_002301535
255761085
0.8313

Populus trichocarpa

6324

ADF30190
294713705
0.8119

Brassica napus

6325
8138

124-144
XP_003543825
356550908
1

Glycine max

6326
8139

752-772
XP_003543825
356550908
1

Glycine max

6327
8140

XP_003556814
356577399
0.813

Glycine max

6328
8141

360-380
XP_003549039
356561539
1

Glycine max

6329
8142

XP_003533180
356529191
0.8282

Glycine max

6330
8143

614-634
XP_003541563
356546291
1

Glycine max

6331
8144

XP_003545791
356554924
0.805

Glycine max

6332
8145

905-925
XP_003556814
356577399
1

Glycine max

6333
8146

XP_003543825
356550908
0.8659

Glycine max

6334
8147

141-161
XP_003518351
356499037
1

Glycine max

6335
8148

2016-2036
XP_003538988
356541033
1

Glycine max

6336
8149

XP_003607189
357473808
0.7407

Medicago truncatula

6337
8150

XP_003604038
357467506
0.7035

Medicago truncatula

6338
8151

839-859
XP_003526354
356515330
1

Glycine max

6339
8152

XP_003523913
356510372
0.9333

Glycine max

6340
8153

842-862
XP_003523913
356510372
1

Glycine max

6341
8154

XP_003526354
356515330
0.9333

Glycine max

6342
8155

926-946
XP_003545791
356554924
1

Glycine max

6343
8156

XP_003541563
356546291
0.8662

Glycine max

6344
8157

ath-
334-354
XP_003554567
356572827
1

Glycine max

6345
8158

miR159b

XP_003521605
356505654
0.9723

Glycine max

6346
8159

XP_003521606
356505656
0.9723

Glycine max

6347
8160

XP_003626013
357511448
0.8949

Medicago truncatula

6348
8161

XP_002272575
225437676
0.8423

Vitis vinifera

6349
8162

CBI37003
270254427
0.8409

Vitis vinifera

6350
8163

XP_002515224
255761086
0.8382

Ricinus communis

6351

CAN71135
147786943
0.8354

Vitis vinifera

6352
8164

XP_002301535
255761085
0.8313

Populus trichocarpa

6353

ADF30190
294713705
0.8119

Brassica napus

6354
8165

405-425
XP_003542140
356547479
1

Glycine max

6355
8166

XP_003546908
356557204
0.8696

Glycine max

6356
8167

305-325
XP_003541823
356546825
1

Glycine max

6357
8168

124-144
XP_003543825
356550908
1

Glycine max

6358
8169

XP_003556814
356577399
0.813

Glycine max

6359
8170

839-859
XP_003526354
356515330
1

Glycine max

6360
8171

XP_003523913
356510372
0.9333

Glycine max

6361
8172

2079-2099
XP_003538988
356541033
1

Glycine max

6362
8173

XP_003607189
357473808
0.7407

Medicago truncatula

6363
8174

XP_003604038
357467506
0.7035

Medicago truncatula

6364
8175

614-634
XP_003541563
356546291
1

Glycine max

6365
8176

XP_003545791
356554924
0.805

Glycine max

6366
8177

905-925
XP_003556814
356577399
1

Glycine max

6367
8178

XP_003543825
356550908
0.8659

Glycine max

6368
8179

926-946
XP_003545791
356554924
1

Glycine max

6369
8180

XP_003541563
356546291
0.8662

Glycine max

6370
8181

842-862
XP_003523913
356510372
1

Glycine max

6371
8182

XP_003526354
356515330
0.9333

Glycine max

6372
8183

ath-
251-271
NP_001236122
351727005
1

Glycine max

6373
8184

miR159c

ACU21384
255642238
0.7676

Glycine max

6374
8185

46-66
XP_003519140
356500640
1

Glycine max

6375
8186

XP_003549552
356562588
0.8757

Glycine max

6376
8187

XP_003610353
357480134
0.9006

Medicago truncatula

6377
8188

817-837
XP_003525997
356514606
1

Glycine max

6378
8189

XP_003540066
356543230
0.8441

Glycine max

6379
8190

289-309
XP_003518627
356499601
1

Glycine max

6380
8191

XP_003542153
356547506
0.9478

Glycine max

6381
8192

ADN33938
307136081
0.7937

Cucumis melo

6382
8193

subsp. melo

XP_002518919
255761086
0.7755

Ricinus communis

6383

XP_002279642
225426567
0.7755

Vitis vinifera

6384
8194

XP_002870592
297853636
0.7664

Arabidopsis lyrata

6385

subsp. lyrata

NP_199024
30693991
0.7642

Arabidopsis thaliana

6386
8195

AAM63843
21405504
0.7596

Arabidopsis thaliana

6387
8196

XP_002299422
255761085
0.7528

Populus trichocarpa

6388

XP_002303695
255761085
0.7574

Populus trichocarpa

6389

124-144
XP_003543825
356550908
1

Glycine max

6390
8197

XP_003556814
356577399
0.813

Glycine max

6391
8198

461-481
XP_003542153
356547506
1

Glycine max

6392
8199

XP_003518627
356499601
0.9436

Glycine max

6393
8200

1162-1182
XP_003531162
356525093
1

Glycine max

6394
8201

XP_003524148
356510852
0.7692

Glycine max

6395
8202

839-859
XP_003526354
356515330
1

Glycine max

6396
8203

XP_003523913
356510372
0.9333

Glycine max

6397
8204

495-515
XP_003524148
356510852
1

Glycine max

6398
8205

XP_003531162
356525093
0.7762

Glycine max

6399
8206

949-969
XP_003547199
356557800
1

Glycine max

6400
8207

XP_003541668
356546507
0.8748

Glycine max

6401
8208

614-634
XP_003541563
356546291
1

Glycine max

6402
8209

XP_003545791
356554924
0.805

Glycine max

6403
8210

905-925
XP_003556814
356577399
1

Glycine max

6404
8211

XP_003543825
356550908
0.8659

Glycine max

6405
8212

2016-2036
XP_003538988
356541033
1

Glycine max

6406
8213

XP_003607189
357473808
0.7407

Medicago truncatula

6407
8214

XP_003604038
357467506
0.7035

Medicago truncatula

6408
8215

1330-1350
XP_003541668
356546507
1

Glycine max

6409
8216

XP_003547199
356557800
0.8383

Glycine max

6410
8217

842-862
XP_003523913
356510372
1

Glycine max

6411
8218

XP_003526354
356515330
0.9333

Glycine max

6412
8219

926-946
XP_003545791
356554924
1

Glycine max

6413
8220

XP_003541563
356546291
0.8662

Glycine max

6414
8221

ath-
288-307
AES96257
357486022
1

Medicago truncatula

6415
8222

miRf10240-

XP_003613299
357486022
1

Medicago truncatula

6416
8223

akr

XP_003517816
356497943
0.9446

Glycine max

6417
8224

XP_003519545
356501464
0.9418

Glycine max

6418
8225

XP_003517817
356497945
0.9446

Glycine max

6419
8226

XP_003530656
356524070
0.8809

Glycine max

6420
8227

XP_003551180
356565911
0.8753

Glycine max

6421
8228

XP_002531401
255761086
0.8421

Ricinus communis

6422

XP_003628672
357516766
0.8504

Medicago truncatula

6423
8229

XP_002266222
225424743
0.8393

Vitis vinifera

6424
8230

CAN76955
147776916
0.8338

Vitis vinifera

6425
8231

1503-1522
XP_003547951
356559326
1

Glycine max

6426
8232

ACU23369
255645751
0.998

Glycine max

6427
8233

XP_003547950
356559324
0.8408

Glycine max

6428
8234

ACU18289
255635883
0.8388

Glycine max

6429
8235

XP_003629354
357518130
0.7531

Medicago truncatula

6430
8236

XP_002531509
255761086
0.7204

Ricinus communis

6431

ABK95760
118487875
0.7245

Populus trichocarpa

6432
8237

XP_003612122
357483670
0.7327

Medicago truncatula

6433
8238

XP_002333330
255761085
0.7122

Populus trichocarpa

6434

XP_002310843
255761085
0.702

Populus trichocarpa

6435

115-134
AES78100
357503186
1

Medicago truncatula

6436
8239

XP_003621882
357503186
1

Medicago truncatula

6437
8240

95-114
AET03830
357518130
1

Medicago truncatula

6438
8241

XP_003547951
356559326
0.811

Glycine max

6439
8242

487-506
NP_001238238
351721588
1

Glycine max

6440
8243

NP_001237618
351725834
0.9947

Glycine max

6441
8244

ACU16478
255632239
0.9679

Glycine max

6442
8245

XP_003519150
356500660
0.7968

Glycine max

6443
8246

149-168
XP_003531007
356524781
1

Glycine max

6444
8247

XP_003528405
356519488
0.9391

Glycine max

6445
8248

XP_003608343
357476114
0.8746

Medicago truncatula

6446
8249

XP_002264303
225429239
0.7885

Vitis vinifera

6447
8250

CBI35448
270252044
0.7885

Vitis vinifera

6448

XP_002518318
255761086
0.767

Ricinus communis

6449

NP_201251
145359643
0.7742

Arabidopsis thaliana

6450
8251

XP_002866616
297853636
0.7778

Arabidopsis lyrata

6451

subsp. lyrata

ABK95206
118486740
0.7527

Populus trichocarpa

6452
8252

XP_002313210
255761085
0.7491

Populus trichocarpa

6453

214-233
XP_003525932
356514476
1

Glycine max

6454
8253

700-719
XP_003523287
356509093
1

Glycine max

6455
8254

XP_003526789
356516210
0.9406

Glycine max

6456
8255

XP_003518282
356498893
0.7406

Glycine max

6457
8256

XP_003544853
356553012
0.7406

Glycine max

6458
8257

ath-
587-606
XP_003543893
356551052
1

Glycine max

6459
8258

miRf10368-

XP_003554723
356573142
0.9659

Glycine max

6460
8259

akr

ACJ85427
357496652
0.8447

Medicago truncatula

6461
8260

XP_003531841
356526470
0.8333

Glycine max

6462
8261

XP_003552550
356568703
0.8106

Glycine max

6463
8262

XP_002284521
225457306
0.7765

Vitis vinifera

6464
8263

XP_002323884
255761085
0.7765

Populus trichocarpa

6465

XP_002284184
225452935
0.7803

Vitis vinifera

6466
8264

XP_002306045
255761085
0.7424

Populus trichocarpa

6467

XP_002526069
255761086
0.7424

Ricinus communis

6468

456-475
XP_003539013
356541084
1

Glycine max

6469
8265

XP_003540676
356544475
0.8768

Glycine max

6470
8266

XP_003539012
356541082
0.7183

Glycine max

6471
8267

685-704
NP_001235161
351721419
1

Glycine max

6472
8268

XP_003548144
356559717
0.9705

Glycine max

6473
8269

XP_003548143
356559715
0.7785

Glycine max

6474
8270

BAB86923
19911192
0.711

Vigna angularis

6475
8271

685-704
NP_001235161
351721419
1

Glycine max

6476
8272

3330-3349
XP_003551446
356566453
1

Glycine max

6477

347-366
XP_003540896
356544921
1

Glycine max

6478
8273

XP_003533477
356529802
0.7278

Glycine max

6479
8274

XP_003607171
357473772
0.8086

Medicago truncatula

6480
8275

XP_002533189
255761086
0.7412

Ricinus communis

6481

XP_002279051
225428040
0.7305

Vitis vinifera

6482
8276

XP_003522637
356507769
0.7466

Glycine max

6483
8277

XP_003526942
356516520
0.7035

Glycine max

6484
8278

88-107
XP_002533189
255761086
1

Ricinus communis

6485

XP_003540896
356544921
0.7035

Glycine max

6486
8279

XP_002270345
225457886
0.7008

Vitis vinifera

6487
8280

589-608
XP_003518505
356499351
1

Glycine max

6488
8281

75-94
XP_002312957
255761085
1

Populus trichocarpa

6489

XP_002306186
255761085
0.9181

Populus trichocarpa

6490

CAN67732
147790359
0.8836

Vitis vinifera

6491
8282

682-701
XP_003556840
356577453
1

Glycine max

6492
8283

XP_003519003
356500365
0.7261

Glycine max

6493
8284

XP_003542320
356547854
0.7261

Glycine max

6494
8285

220-239
XP_003527967
356518600
1

Glycine max

6495
8286

XP_003523399
356509321
0.7599

Glycine max

6496
8287

1834-1853
XP_003539709
356542508
1

Glycine max

6497
8288

XP_003538207
356539441
0.9136

Glycine max

6498
8289

XP_003606389
357472208
0.8066

Medicago truncatula

6499
8290

NP_001238028
351722912
0.7617

Glycine max

6500
8291

XP_003543464
356550174
0.7375

Glycine max

6501
8292

XP_003539710
356542510
0.8394

Glycine max

6502
8293

XP_002530284
255761086
0.7081

Ricinus communis

6503

0-19
AES82704
357512394
1

Medicago truncatula

6504

XP_003521781
356506009
0.8339

Glycine max

6505

CBI28651
270241399
0.747

Vitis vinifera

6506

XP_002267871
225430630
0.7263

Vitis vinifera

6507

XP_003553769
356571203
0.8332

Glycine max

6508

1104-1123
XP_003518991
356500340
1

Glycine max

6509
8294

XP_003535145
356533178
0.8841

Glycine max

6510
8295

XP_003535146
356533180
0.8509

Glycine max

6511
8296

439-458
XP_003526441
356515505
1

Glycine max

6512
8297

XP_003522648
356507791
0.9351

Glycine max

6513
8298

XP_003522647
356507789
0.8643

Glycine max

6514
8299

XP_002284618
225430104
0.7935

Vitis vinifera

6515
8300

XP_003526440
356515503
0.8525

Glycine max

6516
8301

XP_002284775
225445329
0.7788

Vitis vinifera

6517
8302

XP_002515572
255761086
0.7699

Ricinus communis

6518

AES73734
357466396
0.7876

Medicago truncatula

6519
8303

1245-1264
NP_001238286
351722970
1

Glycine max

6520
8304

BAG72094
207367147
0.9584

Glycine max

6521
8305

ACE79196
190586158
0.9558

Glycine max

6522
8306

ACE79197
190586160
0.9019

Glycine max

6523
8307

XP_003555766
356575272
0.8983

Glycine max

6524
8308

P93673

0.8453

Lathyrus sativus

6525

P15001

0.8453

Pisum sativum

6526

AES61525
357441992
0.8426

Medicago truncatula

6527
8309

XP_002278610
225450404
0.7772

Vitis vinifera

6528
8310

ACC60969
183239021
0.7763

Vitis riparia

6529

177-196
XP_002306186
255761085
1

Populus trichocarpa

6530

XP_002312957
255761085
0.7553

Populus trichocarpa

6531

439-458
XP_003522648
356507791
1

Glycine max

6532
8311

XP_003526441
356515505
0.9351

Glycine max

6533
8312

NP_189078
42565156
0.7168

Arabidopsis thaliana

6534
8313

200-219
XP_003597608
357454654
1

Medicago truncatula

6535
8314

ABN08398
357454654
1

Medicago truncatula

6536
8315

XP_003546676
356556729
0.8954

Glycine max

6537
8316

XP_003543598
356550445
0.8942

Glycine max

6538
8317

XP_003531618
356526020
0.8609

Glycine max

6539
8318

XP_003529875
356522481
0.8537

Glycine max

6540
8319

CAA07236
316995680
0.849

Cicer arietinum

6541
8320

ABK96254
118488889
0.8121

Populus trichocarpa ×
6542
8321

Populus deltoides

CAC44500
14970838
0.8157

Fragaria × ananassa
6543
8322

XP_002327432
255761085
0.8109

Populus trichocarpa

6544

ABV32545
157313303
0.824

Prunus persica

6545
8323

20-39
XP_003538511
356540064
1

Glycine max

6546

XP_003540789
356544707
0.9546

Glycine max

6547

AES88424
357471884
0.8454

Medicago truncatula

6548

XP_002328167
255761085
0.78

Populus trichocarpa

6549

XP_002529065
255761086
0.7791

Ricinus communis

6550

XP_002269920
225436294
0.7732

Vitis vinifera

6551

1726-1745
XP_003538207
356539441
1

Glycine max

6552
8324

XP_003539709
356542508
0.901

Glycine max

6553
8325

AES88586
357472208
0.8038

Medicago truncatula

6554
8326

XP_003538208
356539443
0.8362

Glycine max

6555
8327

ath-
137-156
XP_003540953
356545038
1

Glycine max

6556
8328

miRf10763-

XP_003537828
356538677
0.9174

Glycine max

6557
8329

akr
246-265
ACU17625
255634523
1

Glycine max

6558
8330

XP_003520499
356503402
0.9227

Glycine max

6559
8331

XP_003554133
356571948
0.8886

Glycine max

6560
8332

448-467
XP_003555015
356573740
1

Glycine max

6561
8333

145-164
XP_002267145
225438252
1

Vitis vinifera

6562
8334

XP_003517356
356497006
0.8121

Glycine max

6563
8335

XP_003539260
356541595
0.8166

Glycine max

6564
8336

XP_003611551
357482528
0.7919

Medicago truncatula

6565
8337

NP_566927
42565786
0.7696

Arabidopsis thaliana

6566
8338

XP_002877705
297853636
0.774

Arabidopsis lyrata

6567

subsp. lyrata

XP_002285420
225458889
0.8076

Vitis vinifera

6568
8339

AAM61258
21403407
0.7673

Arabidopsis thaliana

6569
8340

NP_201393
30698164
0.7405

Arabidopsis thaliana

6570
8341

AAL32554
17064799
0.7383

Arabidopsis thaliana

6571
8342

523-542
XP_003524815
356512212
1

Glycine max

6572
8343

356528136
0.981

Glycine max

6573

136-155
XP_003520083
356502552
1

Glycine max

6574
8344

XP_003517797
356497905
0.8522

Glycine max

6575
8345

993-1012
AET34792
356650815
1

Pisum sativum

6576
8346

AET34790
356650811
0.9979

Pisum sativum

6577
8347

AET34786
356650803
0.9358

Medicago truncatula

6578
8348

XP_003625012
357509446
0.9337

Medicago truncatula

6579
8349

AET34796
356650823
0.8654

Glycine max

6580
8350

XP_003521136
356504705
0.8551

Glycine max

6581
8351

AEM62768
343794555
0.8778

Lotus japonicus

6582
8352

XP_002275980
225441316
0.8282

Vitis vinifera

6583
8353

CAN80112
147852377
0.8282

Vitis vinifera

6584
8354

XP_002308905
255761085
0.824

Populus trichocarpa

6585

223-242
XP_003520499
356503402
1

Glycine max

6586
8355

ACU17625
255634523
0.9878

Glycine max

6587
8356

217-236
XP_003526336
356515293
1

Glycine max

6588
8357

XP_003540834
356544797
0.7611

Glycine max

6589
8358

241-260
XP_003519685
356501746
1

Glycine max

6590
8359

ACU23918
255646896
0.9948

Glycine max

6591
8360

XP_003545065
356553441
0.8093

Glycine max

6592
8361

26-45
CBI33098
270247736
1

Vitis vinifera

6593
8362

237-256
AAF73257
8132346
1

Pisum sativum

6594
8363

XP_003523778
356510099
0.9489

Glycine max

6595
8364

XP_003527981
356518628
0.9382

Glycine max

6596
8365

CAN70091
123701299
0.8817

Vitis vinifera

6597
8366

Q40517

0.8817

Nicotiana tabacum

6598

NP_001233761
350539780
0.8737

Solanum lycopersicum

6599
8367

Q40884

0.8737

Petunia × hybrida
6600

XP_002302017
255761085
0.871

Populus trichocarpa

6601

XP_002510434
255761086
0.8737

Ricinus communis

6602

360-379
XP_003539534
356542151
1

Glycine max

6603
8368

XP_003543335
356549913
0.8571

Glycine max

6604
8369

26-45
XP_003601902
357463240
1

Medicago truncatula

6605
8370

XP_003538598
356540240
0.8571

Glycine max

6606
8371

XP_003551994
356567575
0.8521

Glycine max

6607
8372

XP_003531333
356525440
0.8045

Glycine max

6608
8373

XP_003525038
356512665
0.797

Glycine max

6609
8374

XP_002530039
255761086
0.7845

Ricinus communis

6610

XP_002314593
255761085
0.7694

Populus trichocarpa

6611

XP_002282407
225424658
0.7669

Vitis vinifera

6612
8375

XP_002311761
255761085
0.7669

Populus trichocarpa

6613

CBI16790
270228074
0.7569

Vitis vinifera

6614

502-521
NP_001234991
351723886
1

Glycine max

6615
8376

XP_003552315
356568227
0.9954

Glycine max

6616
8377

XP_003543429
356550102
0.9815

Glycine max

6617
8378

NP_001236946
351721215
0.9769

Glycine max

6618
8379

XP_003596822
357453090
0.9444

Medicago truncatula

6619
8380

XP_003611340
357482108
0.9444

Medicago truncatula

6620
8381

XP_003600923
357461282
0.9444

Medicago truncatula

6621
8382

NP_001237030
351723638
0.9306

Glycine max

6622
8383

ACG24758
195605855
0.875

Zea mays

6623
8384

ACG33436
195623211
0.875

Zea mays

6624
8385

129-148
ACU17625
255634523
1

Glycine max

6625
8386

290-309
XP_003548849
356561151
1

Glycine max

6626
8387

XP_003519868
356502117
0.8445

Glycine max

6627
8388

XP_003629318
357518058
0.7456

Medicago truncatula

6628
8389

237-256
XP_003527981
356518628
1

Glycine max

6629
8390

AAF73257
8132346
0.9407

Pisum sativum

6630
8391

ABA00652
74231015
0.8814

Gossypium hirsutum

6631
8392

37-56
XP_003547100
356557592
1

Glycine max

6632
8393

XP_003541782
356546742
0.862

Glycine max

6633
8394

XP_003593272
357445988
0.7085

Medicago truncatula

6634
8395

248-267
ACU23918
255646896
1

Glycine max

6635
8396

XP_003519685
356501746
0.9948

Glycine max

6636
8397

ath-
757-777
XP_003629993
357519408
1

Medicago truncatula

6637
8398

miRf11042-

ACJ84083
217071445
0.9917

Medicago truncatula

6638
8399

akr

NP_001235375
351727608
0.8595

Glycine max

6639
8400

NP_001237170
351727706
0.8264

Glycine max

6640
8401

CAA10134
3860332
0.8347

Cicer arietinum

6641
8402

XP_002298184
255761085
0.719

Populus trichocarpa

6642

NP_001237531
351723330
0.7107

Glycine max

6643
8403

XP_003523409
356509341
0.7107

Glycine max

6644
8404

357519363
1

Medicago truncatula

6645

361-381
XP_003592180
357443804
1

Medicago truncatula

6646
8405

XP_003556131
356576015
0.7984

Glycine max

6647
8406

XP_003535589
356534086
0.8

Glycine max

6648
8407

XP_002329680
255761085
0.777

Populus trichocarpa

6649

XP_002276766
225464654
0.7475

Vitis vinifera

6650
8408

CBI22616
270233919
0.7213

Vitis vinifera

6651
8409

1042-1062
XP_003616507
357492436
1

Medicago truncatula

6652
8410

XP_003518398
356499134
0.8512

Glycine max

6653
8411

XP_003545247
356553814
0.8492

Glycine max

6654
8412

XP_003537472
356537955
0.7738

Glycine max

6655
8413

XP_003552860
356569339
0.7718

Glycine max

6656
8414

XP_002524242
255761086
0.7321

Ricinus communis

6657

XP_002277622
225449359
0.7063

Vitis vinifera

6658
8415

520-540
XP_003534554
356531980
1

Glycine max

6659

XP_003552402
356568406
0.9572

Glycine max

6660

XP_003548671
356560791
0.7736

Glycine max

6661

XP_003528847
356520393
0.7732

Glycine max

6662

XP_003623999
357507420
0.7898

Medicago truncatula

6663

XP_003627563
357514548
0.7385

Medicago truncatula

6664

XP_002276245
225454279
0.7367

Vitis vinifera

6665

XP_002511882
255761086
0.7349

Ricinus communis

6666

CAN74059
147789689
0.7228

Vitis vinifera

6667

1412-1432
XP_003556131
356576015
1

Glycine max

6668
8416

XP_003592180
357443804
0.8203

Medicago truncatula

6669
8417

csi-
43-66
XP_003547789
356558998
1

Glycine max

6670
8418

miR3948

XP_003531955
356526700
0.9164

Glycine max

6671
8419

ACU23577
255646183
0.9078

Glycine max

6672
8420

209-232
XP_003540784
356544697
1

Glycine max

6673
8421

XP_003539180
356541429
0.8869

Glycine max

6674
8422

40-63
XP_003556473
356576709
1

Glycine max

6675
8423

XP_003535369
356533638
0.8958

Glycine max

6676
8424

47-70
XP_003527776
356518214
1

Glycine max

6677
8425

77-100
XP_003550061
356563623
1

Glycine max

6678
8426

XP_003525811
356514233
0.9621

Glycine max

6679
8427

XP_002310135
255761085
0.7098

Populus trichocarpa

6680

XP_002523601
255761086
0.7035

Ricinus communis

6681

173-196
XP_003525811
356514233
1

Glycine max

6682
8428

XP_003550061
356563623
0.9591

Glycine max

6683
8429

208-231
XP_003542594
356548408
1

Glycine max

6684
8430

XP_003537062
356537086
0.7495

Glycine max

6685
8431

179-202
XP_003539180
356541429
1

Glycine max

6686
8432

XP_003540784
356544697
0.8923

Glycine max

6687
8433

209-232
BAD18437
47077005
1

Homo sapiens

6688
8434

XP_003522605
356507705
1

Glycine max

6689
8435

XP_003526400
356515423
0.9316

Glycine max

6690
8436

232-255
XP_003537062
356537086
1

Glycine max

6691
8437

XP_003542594
356548408
0.7416

Glycine max

6692
8438

ghr-
37-57
XP_003518096
356498514
1

Glycine max

6693
8439

miR2950

XP_003537196
356537360
0.9379

Glycine max

6694
8440

XP_003516696
356495666
0.7495

Glycine max

6695
8441

909-929
XP_003529456
356521627
1

Glycine max

6696
8442

XP_003556690
356577148
0.8891

Glycine max

6697
8443

XP_003601600
357462636
0.8073

Medicago truncatula

6698
8444

XP_003601595
357462626
0.8073

Medicago truncatula

6699
8445

XP_003601599
357462634
0.8073

Medicago truncatula

6700
8446

XP_003601596
357462628
0.8036

Medicago truncatula

6701
8447

XP_003607816
357475060
0.7764

Medicago truncatula

6702
8448

XP_003601601
357462638
0.7982

Medicago truncatula

6703
8449

XP_003538519
356540080
0.7436

Glycine max

6704
8450

XP_002303974
255761085
0.7491

Populus trichocarpa

6705

1446-1466
BAG68945
197209811
1

Lotus japonicus

6706
8451

XP_003549436
356562352
0.881

Glycine max

6707
8452

AAC09468
6503252
0.8541

Pisum sativum

6708
8453

XP_003541616
356546403
0.8824

Glycine max

6709
8454

XP_003610109
357479646
0.8598

Medicago truncatula

6710
8455

XP_002270732
225446043
0.8116

Vitis vinifera

6711
8456

ACN54324
224551851
0.7805

Gossypium hirsutum

6712
8457

AAK15261
13183565
0.7748

Populus trichocarpa ×
6713
8458

Populus deltoides

CBI17838
270228824
0.7734

Vitis vinifera

6714

XP_002324469
255761085
0.7677

Populus trichocarpa

6715

177-197
XP_003554852
356573405
1

Glycine max

6716
8459

XP_003543493
356550234
0.8998

Glycine max

6717
8460

461-481
XP_003536297
356535528
1

Glycine max

6718
8461

XP_003556292
356576342
0.9205

Glycine max

6719
8462

238-258
XP_003518581
356499506
1

Glycine max

6720
8463

XP_003618091
357495604
0.7113

Medicago truncatula

6721
8464

gma-
75-94
XP_003520455
356503312
1

Glycine max

6722
8465

miR156g

XP_003530170
356523079
0.848

Glycine max

6723
8466

371-390
XP_003553428
356570509
1

Glycine max

6724
8467

XP_003520534
356503475
0.9081

Glycine max

6725
8468

759-778
XP_003520534
356503475
1

Glycine max

6726
8469

XP_003553428
356570509
0.9081

Glycine max

6727
8470

108-127
XP_003553944
356571558
1

Glycine max

6728
8471

XP_003549130
356561725
0.9452

Glycine max

6729
8472

237-256
XP_003551188
356565928
1

Glycine max

6730
8473

XP_003538544
356540131
0.9106

Glycine max

6731
8474

ACU18328
255635963
0.9083

Glycine max

6732
8475

XP_003601767
357462970
0.7064

Medicago truncatula

6733
8476

36-55
AAM12880
20149261
1

Helianthus annuus

6734
8477

CBI28152
270240501
0.991

Vitis vinifera

6735

XP_002284967
225430201
0.991

Vitis vinifera

6736
8478

CBI21000
270231236
0.991

Vitis vinifera

6737

CBI36254
270253379
0.9819

Vitis vinifera

6738
8479

NP_200330
145359269
0.9864

Arabidopsis thaliana

6739
8480

XP_003522628
356507751
0.9819

Glycine max

6740
8481

AEM97804
344189954
0.9864

Dimocarpus longan

6741
8482

XP_002864382
297853636
0.9864

Arabidopsis lyrata

6742

subsp. lyrata

XP_002285307
225442824
0.9819

Vitis vinifera

6743
8483

1020-1039
XP_003532399
356527605
1

Glycine max

6744
8484

XP_003525415
356513426
0.8914

Glycine max

6745
8485

114-133
ACU18105
255635506
1

Glycine max

6746
8486

756-775
XP_003518080
356498481
1

Glycine max

6747
8487

XP_003551421
356566402
0.8408

Glycine max

6748
8488

118-137
XP_003549130
356561725
1

Glycine max

6749
8489

XP_003553944
356571558
0.9452

Glycine max

6750
8490

662-681
XP_003550514
356564545
1

Glycine max

6751
8491

XP_003528668
356520027
0.9389

Glycine max

6752
8492

XP_003541638
356546447
0.8242

Glycine max

6753
8493

XP_003547234
356557871
0.808

Glycine max

6754
8494

XP_003594096
357447640
0.803

Medicago truncatula

6755
8495

XP_002279739
225432499
0.7382

Vitis vinifera

6756
8496

CAN70618
147801938
0.7406

Vitis vinifera

6757
8497

XP_002314424
255761085
0.7406

Populus trichocarpa

6758

XP_002516799
255761086
0.7219

Ricinus communis

6759

XP_002312735
255761085
0.7157

Populus trichocarpa

6760

691-710
XP_003525415
356513426
1

Glycine max

6761
8498

XP_003532399
356527605
0.9051

Glycine max

6762
8499

114-133
XP_003538544
356540131
1

Glycine max

6763
8500

XP_003551188
356565928
0.9097

Glycine max

6764
8501

1068-1087
XP_003525436
356513468
1

Glycine max

6765
8502

XP_003550708
356564947
0.9204

Glycine max

6766
8503

XP_003522278
356507037
0.7595

Glycine max

6767
8504

999-1018
XP_003550708
356564947
1

Glycine max

6768
8505

XP_003525436
356513468
0.9373

Glycine max

6769
8506

1098-1117
XP_003520128
356502644
1

Glycine max

6770
8507

XP_003517860
356498034
0.9129

Glycine max

6771
8508

179-198
XP_003523155
356508826
1

Glycine max

6772
8509

711-730
XP_003551421
356566402
1

Glycine max

6773
8510

XP_003518080
356498481
0.7962

Glycine max

6774
8511

gma-
164-183
XP_003549130
356561725
1

Glycine max

6775
8512

miR157c

XP_003553944
356571558
0.9452

Glycine max

6776
8513

593-612
NP_001236309
351724988
1

Glycine max

6777
8514

XP_003529339
356521389
0.838

Glycine max

6778
8515

gma-
305-325
XP_003551790
356567161
1

Glycine max

6779
8516

miR159a-
305-325
XP_003541823
356546825
1

Glycine max

6780
8517

3p
124-144
XP_003543825
356550908
1

Glycine max

6781
8518

XP_003556814
356577399
0.813

Glycine max

6782
8519

405-425
XP_003542140
356547479
1

Glycine max

6783
8520

XP_003546908
356557204
0.8696

Glycine max

6784
8521

305-325
XP_003541823
356546825
1

Glycine max

6785
8522

124-144
XP_003543825
356550908
1

Glycine max

6786
8523

839-859
XP_003526354
356515330
1

Glycine max

6787
8524

XP_003523913
356510372
0.9333

Glycine max

6788
8525

73-93
XP_003535315
356533526
1

Glycine max

6789
8526

XP_003555178
356574075
0.9462

Glycine max

6790
8527

XP_003591226
357441896
0.7849

Medicago truncatula

6791
8528

XP_002512536
255761086
0.7465

Ricinus communis

6792

CBI39621
270257428
0.7465

Vitis vinifera

6793
8529

CAP59645
163913883
0.7558

Vitis vinifera

6794
8530

XP_002277312
225450534
0.7496

Vitis vinifera

6795
8531

XP_002280462
225432056
0.7404

Vitis vinifera

6796
8532

CAP59646
163913885
0.7512

Vitis vinifera

6797
8533

CAN63178
123711273
0.7373

Vitis vinifera

6798
8534

614-634
XP_003541563
356546291
1

Glycine max

6799
8535

XP_003545791
356554924
0.805

Glycine max

6800
8536

905-925
XP_003556814
356577399
1

Glycine max

6801
8537

XP_003543825
356550908
0.8659

Glycine max

6802
8538

2016-2036
XP_003538988
356541033
1

Glycine max

6803
8539

XP_003607189
357473808
0.7407

Medicago truncatula

6804
8540

XP_003604038
357467506
0.7035

Medicago truncatula

6805
8541

926-946
XP_003545791
356554924
1

Glycine max

6806
8542

XP_003541563
356546291
0.8662

Glycine max

6807
8543

842-862
XP_003523913
356510372
1

Glycine max

6808
8544

XP_003526354
356515330
0.9333

Glycine max

6809
8545

iba-
164-184
XP_003549130
356561725
1

Glycine max

6810
8546

miR157

XP_003553944
356571558
0.9452

Glycine max

6811
8547

238-258
XP_003551188
356565928
1

Glycine max

6812
8548

XP_003538544
356540131
0.9106

Glycine max

6813
8549

ACU18328
255635963
0.9083

Glycine max

6814
8550

XP_003601767
357462970
0.7064

Medicago truncatula

6815
8551

1129-1149
XP_003525415
356513426
1

Glycine max

6816
8552

XP_003532399
356527605
0.9051

Glycine max

6817
8553

898-918
XP_003540473
356544059
1

Glycine max

6818
8554

XP_003543233
356549706
0.9305

Glycine max

6819
8555

ACU24116
255647298
0.9251

Glycine max

6820
8556

118-138
XP_003525436
356513468
1

Glycine max

6821
8557

XP_003550708
356564947
0.9204

Glycine max

6822
8558

XP_003522278
356507037
0.7595

Glycine max

6823
8559

77-97
XP_003520455
356503312
1

Glycine max

6824
8560

XP_003530170
356523079
0.848

Glycine max

6825
8561

498-518
XP_003553428
356570509
1

Glycine max

6826
8562

XP_003520534
356503475
0.9081

Glycine max

6827
8563

50-70
XP_003550708
356564947
1

Glycine max

6828
8564

XP_003525436
356513468
0.9373

Glycine max

6829
8565

170-190
XP_003538544
356540131
1

Glycine max

6830
8566

XP_003551188
356565928
0.9097

Glycine max

6831
8567

593-613
NP_001236309
351724988
1

Glycine max

6832
8568

XP_003529339
356521389
0.838

Glycine max

6833
8569

144-164
XP_002275728
225446415
1

Vitis vinifera

6834
8570

AAY16440
62856978
0.7396

Betula platyphylla

6835
8571

112-132
XP_003522278
356507037
1

Glycine max

6836
8572

396-416
XP_003520128
356502644
1

Glycine max

6837
8573

XP_003517860
356498034
0.9129

Glycine max

6838
8574

181-201
XP_003523155
356508826
1

Glycine max

6839
8575

77-97
XP_003520455
356503312
1

Glycine max

6840
8576

373-393
XP_003553428
356570509
1

Glycine max

6841
8577

761-781
XP_003520534
356503475
1

Glycine max

6842
8578

XP_003553428
356570509
0.9081

Glycine max

6843
8579

110-130
XP_003553944
356571558
1

Glycine max

6844
8580

239-259
XP_003551188
356565928
1

Glycine max

6845
8581

1022-1042
XP_003532399
356527605
1

Glycine max

6846
8582

XP_003525415
356513426
0.8914

Glycine max

6847
8583

593-613
NP_001236309
351724988
1

Glycine max

6848
8584

116-136
ACU18105
255635506
1

Glycine max

6849
8585

758-778
XP_003518080
356498481
1

Glycine max

6850
8586

XP_003551421
356566402
0.8408

Glycine max

6851
8587

120-140
XP_003549130
356561725
1

Glycine max

6852
8588

693-713
XP_003525415
356513426
1

Glycine max

6853
8589

656-676
XP_003517558
356497418
1

Glycine max

6854
8590

XP_003537666
356538348
0.9182

Glycine max

6855
8591

XP_003539001
356541059
0.7453

Glycine max

6856
8592

XP_003540042
356543182
0.7421

Glycine max

6857
8593

116-136
XP_003538544
356540131
1

Glycine max

6858
8594

1070-1090
XP_003525436
356513468
1

Glycine max

6859
8595

404-424
XP_003526029
356514674
1

Glycine max

6860
8596

XP_003540122
356543345
0.8919

Glycine max

6861
8597

1100-1120
XP_003520128
356502644
1

Glycine max

6862
8598

1001-1021
XP_003550708
356564947
1

Glycine max

6863
8599

243-263
XP_003540122
356543345
1

Glycine max

6864
8600

XP_003526029
356514674
0.8684

Glycine max

6865
8601

555-575
XP_003524444
356511459
1

Glycine max

6866
8602

XP_003533073
356528975
0.7951

Glycine max

6867
8603

181-201
XP_003523155
356508826
1

Glycine max

6868
8604

713-733
XP_003551421
356566402
1

Glycine max

6869
8605

XP_003518080
356498481
0.7962

Glycine max

6870
8606

mdm-
517-537
XP_003528897
356520494
1

Glycine max

6871
8607

miR482a-

ACU19201
255637757
0.988

Glycine max

6872
8608

5p

XP_003529981
356522693
0.7784

Glycine max

6873
8609

XP_003521936
356506325
0.7695

Glycine max

6874
8610

626-646
XP_003554327
356572340
1

Glycine max

6875
8611

XP_003521338
356505115
0.984

Glycine max

6876
8612

XP_003554326
356572338
1

Glycine max

6877
8613

XP_003521337
356505113
0.984

Glycine max

6878
8614

AES81717
357510420
0.869

Medicago truncatula

6879
8615

ACJ84304
217071887
0.8658

Medicago truncatula

6880
8616

ABN05708
46063642
0.8594

Medicago truncatula

6881
8617

AES84650
339649035
0.8339

Medicago truncatula

6882
8618

XP_003542462
356548141
0.8115

Glycine max

6883
8619

XP_003542461
356548139
0.8115

Glycine max

6884
8620

204-224
XP_003625987
357511396
1

Medicago truncatula

6885
8621

AES82205
357511396
1

Medicago truncatula

6886
8622

XP_003520757
356503939
0.8043

Glycine max

6887
8623

XP_003554555
356572802
0.802

Glycine max

6888
8624

175-195
XP_003554555
356572802
1

Glycine max

6889
8625

XP_002271147
225437597
0.7397

Vitis vinifera

6890
8626

XP_002515261
255761086
0.7188

Ricinus communis

6891

716-736
XP_003539613
356542313
1

Glycine max

6892
8627

XP_003543221
356549682
0.8767

Glycine max

6893
8628

778-798
XP_003625499
357510420
1

Medicago truncatula

6894
8629

XP_003554327
356572340
0.8395

Glycine max

6895
8630

XP_003537175
356537316
0.8056

Glycine max

6896
8631

osa-
500-520
XP_003541668
356546507
1

Glycine max

6897
8632

miR159e

XP_003547199
356557800
0.8383

Glycine max

6898
8633

495-515
XP_003524148
356510852
1

Glycine max

6899
8634

XP_003531162
356525093
0.7762

Glycine max

6900
8635

26-46
XP_003547199
356557800
1

Glycine max

6901
8636

124-144
XP_003543825
356550908
1

Glycine max

6902
8637

XP_003556814
356577399
0.813

Glycine max

6903
8638

817-837
XP_003525997
356514606
1

Glycine max

6904
8639

XP_003540066
356543230
0.8441

Glycine max

6905
8640

289-309
XP_003518627
356499601
1

Glycine max

6906
8641

XP_003542153
356547506
0.9478

Glycine max

6907
8642

ADN33938
307136081
0.7937

Cucumis melo

6908
8643

subsp. melo

XP_002518919
255761086
0.7755

Ricinus communis

6909

XP_002279642
225426567
0.7755

Vitis vinifera

6910
8644

XP_002870592
297853636
0.7664

Arabidopsis lyrata

6911

subsp. lyrata

NP_199024
30693991
0.7642

Arabidopsis thaliana

6912
8645

AAM63843
21405504
0.7596

Arabidopsis thaliana

6913
8646

XP_002299422
255761085
0.7528

Populus trichocarpa

6914

XP_002303695
255761085
0.7574

Populus trichocarpa

6915

124-144
XP_003543825
356550908
1

Glycine max

6916
8647

461-481
XP_003542153
356547506
1

Glycine max

6917
8648

XP_003518627
356499601
0.9436

Glycine max

6918
8649

1162-1182
XP_003531162
356525093
1

Glycine max

6919
8650

XP_003524148
356510852
0.7692

Glycine max

6920
8651

839-859
XP_003526354
356515330
1

Glycine max

6921
8652

XP_003523913
356510372
0.9333

Glycine max

6922
8653

495-515
XP_003524148
356510852
1

Glycine max

6923
8654

949-969
XP_003547199
356557800
1

Glycine max

6924
8655

614-634
XP_003541563
356546291
1

Glycine max

6925
8656

XP_003545791
356554924
0.805

Glycine max

6926
8657

905-925
XP_003556814
356577399
1

Glycine max

6927
8658

XP_003543825
356550908
0.8659

Glycine max

6928
8659

2016-2036
XP_003538988
356541033
1

Glycine max

6929
8660

XP_003607189
357473808
0.7407

Medicago truncatula

6930
8661

XP_003604038
357467506
0.7035

Medicago truncatula

6931
8662

1330-1350
XP_003541668
356546507
1

Glycine max

6932
8663

842-862
XP_003523913
356510372
1

Glycine max

6933
8664

XP_003526354
356515330
0.9333

Glycine max

6934
8665

926-946
XP_003545791
356554924
1

Glycine max

6935
8666

XP_003541563
356546291
0.8662

Glycine max

6936
8667

osa-
124-144
XP_003543825
356550908
1

Glycine max

6937
8668

miR159f

XP_003556814
356577399
0.813

Glycine max

6938
8669

305-325
XP_003541823
356546825
1

Glycine max

6939
8670

124-144
XP_003543825
356550908
1

Glycine max

6940
8671

839-859
XP_003526354
356515330
1

Glycine max

6941
8672

XP_003523913
356510372
0.9333

Glycine max

6942
8673

614-634
XP_003541563
356546291
1

Glycine max

6943
8674

XP_003545791
356554924
0.805

Glycine max

6944
8675

905-925
XP_003556814
356577399
1

Glycine max

6945
8676

2016-2036
XP_003538988
356541033
1

Glycine max

6946
8677

XP_003607189
357473808
0.7407

Medicago truncatula

6947
8678

XP_003604038
357467506
0.7035

Medicago truncatula

6948
8679

842-862
XP_003523913
356510372
1

Glycine max

6949
8680

XP_003526354
356515330
0.9333

Glycine max

6950
8681

926-946
XP_003545791
356554924
1

Glycine max

6951
8682

XP_003541563
356546291
0.8662

Glycine max

6952
8683

osa-
796-816
ABC49719
84028520
1

Arachis hypogaea

6953
8684

miR1850.1

NP_001236448
351734389
0.9568

Glycine max

6954
8685

XP_003535034
356532953
0.9496

Glycine max

6955
8686

XP_003594440
357448328
0.8993

Medicago truncatula

6956
8687

ACJ84098
217071475
0.8921

Medicago truncatula

6957
8688

XP_003629907
357519236
0.8705

Medicago truncatula

6958
8689

ABD63906
89212811
0.8921

Gossypium hirsutum

6959
8690

ABD66505
89276296
0.8849

Gossypium hirsutum

6960
8691

ADN34239
307136431
0.8633

Cucumis melo

6961
8692

subsp. melo

ABD66508
89276302
0.8849

Gossypium hirsutum

6962
8693

746-766
XP_003535034
356532953
1

Glycine max

6963
8694

CAJ38384
106879600
0.8849

Plantago major

6964
8695

XP_002299887
255761085
0.9065

Populus trichocarpa

6965

17-37
XP_003534041
356530948
1

Glycine max

6966
8696

117-137
XP_003524950
356512486
1

Glycine max

6967
8697

243-263
XP_003548988
356561437
1

Glycine max

6968
8698

XP_003548995
356561451
0.7944

Glycine max

6969
8699

XP_003548992
356561445
0.728

Glycine max

6970
8700

XP_003548994
356561449
0.7846

Glycine max

6971
8701

XP_003548993
356561447
0.921

Glycine max

6972
8702

XP_003548986
356561433
0.8079

Glycine max

6973
8703

XP_003548991
356561443
0.807

Glycine max

6974
8704

XP_003617757
357494936
0.8241

Medicago truncatula

6975
8705

XP_003616414
357492250
0.7971

Medicago truncatula

6976
8706

53-73
XP_003552127
356567847
1

Glycine max

6977
8707

XP_003532277
356527355
0.9545

Glycine max

6978
8708

ADB79567
284156655
0.7995

Arachis hypogaea

6979
8709

XP_002262721
225470104
0.7433

Vitis vinifera

6980
8710

ABX82799
296916970
0.7219

Jatropha curcas

6981
8711

XP_002532744
255761086
0.7166

Ricinus communis

6982

XP_002863277
297853636
0.7112

Arabidopsis lyrata

6983

subsp. lyrata

AAC49002
595956
0.7059

Brassica rapa

6984
8712

141-161
XP_003555849
356575439
1

Glycine max

6985
8713

XP_003592693
357444830
0.8289

Medicago truncatula

6986
8714

XP_002529805
255761086
0.7425

Ricinus communis

6987

XP_002306206
255761085
0.7331

Populus trichocarpa

6988

XP_002264823
225467465
0.7221

Vitis vinifera

6989
8715

XP_002278507
225428128
0.719

Vitis vinifera

6990
8716

XP_002312937
255761085
0.7159

Populus trichocarpa

6991

2940-2960
XP_003550417
356564348
1

Glycine max

6992
8717

XP_003523561
356509653
0.7407

Glycine max

6993
8718

XP_003523562
356509655
0.7323

Glycine max

6994
8719

osa-
90-110
XP_003520359
356503118
1

Glycine max

6995
8720

miR1858a

XP_003547751
356558921
0.94

Glycine max

6996
8721

AES83637
339648991
0.7389

Medicago truncatula

6997

XP_003522288
356507057
0.7176

Glycine max

6998
8722

XP_003604071
357467572
0.706

Medicago truncatula

6999
8723

24-44
XP_003553781
356571227
1

Glycine max

7000
8724

NP_001235053
351725668
0.8396

Glycine max

7001
8725

BAF49302
133874197
0.7167

Clitoria ternatea

7002
8726

364-384
XP_003554024
356571724
1

Glycine max

7003
8727

XP_003554025
356571726
0.9937

Glycine max

7004
8728

XP_003548728
356560906
0.9669

Glycine max

7005
8729

XP_003624635
357508692
0.8873

Medicago truncatula

7006
8730

XP_002285720
225435753
0.8202

Vitis vinifera

7007
8731

XP_002281591
225441588
0.8229

Vitis vinifera

7008
8732

XP_003531251
356525273
0.8157

Glycine max

7009
8733

XP_002304857
255761085
0.797

Populus trichocarpa

7010

XP_002299105
255761085
0.7898

Populus trichocarpa

7011

XP_003629733
357518888
0.7987

Medicago truncatula

7012
8734

345-365
XP_003548728
356560906
1

Glycine max

7013
8735

XP_003554024
356571724
0.9704

Glycine max

7014
8736

24-44
XP_003553781
356571227
1

Glycine max

7015
8737

56-76
XP_003521247
356504932
1

Glycine max

7016
8738

XP_003554255
356572193
0.9395

Glycine max

7017
8739

XP_002520726
255761086
0.7893

Ricinus communis

7018

XP_002278267
225437025
0.8136

Vitis vinifera

7019
8740

XP_002307588
255761085
0.7966

Populus trichocarpa

7020

XP_002892149
297853636
0.7215

Arabidopsis lyrata

7021

subsp. lyrata

NP_563676
30678481
0.7361

Arabidopsis thaliana

7022
8741

360-380
NP_001237118
351726189
1

Glycine max

7023
8742

XP_003556230
356576216
0.9243

Glycine max

7024
8743

XP_002529571
255761086
0.7775

Ricinus communis

7025

XP_002315301
255761085
0.7706

Populus trichocarpa

7026

XP_002275232
225449067
0.7752

Vitis vinifera

7027
8744

XP_002312018
255761085
0.7569

Populus trichocarpa

7028

ACM45079
222136858
0.7729

Vitis vinifera

7029
8745

XP_002894135
297853636
0.7729

Arabidopsis lyrata

7030

subsp. lyrata

ABA54870
76782199
0.7638

Fagus sylvatica

7031
8746

CAN73646
123693264
0.7706

Vitis vinifera

7032

277-297
XP_003591923
357443290
1

Medicago truncatula

7033
8747

180-200
XP_003524517
356511610
1

Glycine max

7034
8748

XP_003549799
356563090
0.9173

Glycine max

7035
8749

564-584
NP_001235053
351725668
1

Glycine max

7036
8750

XP_003553781
356571227
0.8319

Glycine max

7037
8751

XP_003626556
357512534
0.7059

Medicago truncatula

7038
8752

ACJ85806
217074891
0.7017

Medicago truncatula

7039
8753

369-389
XP_003530234
356523208
1

Glycine max

7040
8754

XP_003551508
356566578
0.9298

Glycine max

7041
8755

188-208
XP_002529571
255761086
1

Ricinus communis

7042

NP_564534
145336530
0.8046

Arabidopsis thaliana

7043
8756

AAG50662
12321108
0.8023

Arabidopsis thaliana

7044
8757

378-398
XP_003551508
356566578
1

Glycine max

7045
8758

XP_003530234
356523208
0.8745

Glycine max

7046
8759

231-251
XP_003528545
356519775
1

Glycine max

7047
8760

XP_003556667
356577102
0.9204

Glycine max

7048
8761

XP_003607899
357475226
0.7898

Medicago truncatula

7049
8762

156-176
XP_003547641
356558699
1

Glycine max

7050
8763

XP_003548802
356561057
0.9768

Glycine max

7051
8764

141-161
XP_003543554
356550357
1

Glycine max

7052
8765

XP_003554155
356571993
0.932

Glycine max

7053
8766

180-200
XP_003549799
356563090
1

Glycine max

7054
8767

XP_003524517
356511610
0.9176

Glycine max

7055
8768

192-212
XP_003531267
356525308
1

Glycine max

7056
8769

XP_003624841
357509104
0.7936

Medicago truncatula

7057
8770

XP_003522101
356506673
0.7277

Glycine max

7058
8771

132-152
XP_003556667
356577102
1

Glycine max

7059
8772

XP_003528545
356519775
0.9129

Glycine max

7060
8773

psi-
124-144
XP_003543825
356550908
1

Glycine max

7061
8774

miR159

XP_003556814
356577399
0.813

Glycine max

7062
8775

289-309
XP_003518627
356499601
1

Glycine max

7063
8776

XP_003542153
356547506
0.9478

Glycine max

7064
8777

ADN33938
307136081
0.7937

Cucumis melo

7065
8778

subsp. melo

XP_002518919
255761086
0.7755

Ricinus communis

7066

XP_002279642
225426567
0.7755

Vitis vinifera

7067
8779

XP_002870592
297853636
0.7664

Arabidopsis lyrata

7068

subsp. lyrata

NP_199024
30693991
0.7642

Arabidopsis thaliana

7069
8780

AAM63843
21405504
0.7596

Arabidopsis thaliana

7070
8781

XP_002299422
255761085
0.7528

Populus trichocarpa

7071

XP_002303695
255761085
0.7574

Populus trichocarpa

7072

124-144
XP_003543825
356550908
1

Glycine max

7073
8782

461-481
XP_003542153
356547506
1

Glycine max

7074
8783

XP_003518627
356499601
0.9436

Glycine max

7075
8784

839-859
XP_003526354
356515330
1

Glycine max

7076
8785

XP_003523913
356510372
0.9333

Glycine max

7077
8786

614-634
XP_003541563
356546291
1

Glycine max

7078
8787

XP_003545791
356554924
0.805

Glycine max

7079
8788

905-925
XP_003556814
356577399
1

Glycine max

7080
8789

19-39
NP_001236539
351724240
1

Glycine max

7081
8790

NP_001237767
351722762
0.9915

Glycine max

7082
8791

NP_001237736
351721871
0.7778

Glycine max

7083
8792

BAF50740
139005586
0.7436

Apios americana

7084
8793

XP_003623947
357507316
0.7094

Medicago truncatula

7085
8794

2016-2036
XP_003538988
356541033
1

Glycine max

7086
8795

XP_003607189
357473808
0.7407

Medicago truncatula

7087
8796

XP_003604038
357467506
0.7035

Medicago truncatula

7088
8797

926-946
XP_003545791
356554924
1

Glycine max

7089
8798

XP_003541563
356546291
0.8662

Glycine max

7090
8799

842-862
XP_003523913
356510372
1

Glycine max

7091
8800

XP_003526354
356515330
0.9333

Glycine max

7092
8801

pta-
161-180
XP_003549130
356561725
1

Glycine max

7093
8802

miR156a

XP_003553944
356571558
0.9452

Glycine max

7094
8803

106-125
XP_003520455
356503312
1

Glycine max

7095
8804

XP_003530170
356523079
0.848

Glycine max

7096
8805

115-134
XP_003525436
356513468
1

Glycine max

7097
8806

XP_003550708
356564947
0.9204

Glycine max

7098
8807

XP_003522278
356507037
0.7595

Glycine max

7099
8808

47-66
XP_003550708
356564947
1

Glycine max

7100
8809

XP_003525436
356513468
0.9373

Glycine max

7101
8810

495-514
XP_003553428
356570509
1

Glycine max

7102
8811

XP_003520534
356503475
0.9081

Glycine max

7103
8812

303-322
XP_003530747
356524258
1

Glycine max

7104

XP_003553084
356569799
0.9116

Glycine max

7105

393-412
XP_003520128
356502644
1

Glycine max

7106
8813

XP_003517860
356498034
0.9129

Glycine max

7107
8814

109-128
XP_003522278
356507037
1

Glycine max

7108
8815

178-197
XP_003523155
356508826
1

Glycine max

7109
8816

755-774
XP_003518080
356498481
1

Glycine max

7110
8817

XP_003551421
356566402
0.8408

Glycine max

7111
8818

117-136
XP_003549130
356561725
1

Glycine max

7112
8819

734-753
XP_003553428
356570509
1

Glycine max

7113
8820

815-834
XP_003553944
356571558
1

Glycine max

7114
8821

121-140
ACU18105
255635506
1

Glycine max

7115
8822

1067-1086
XP_003525436
356513468
1

Glycine max

7116
8823

106-125
XP_003520455
356503312
1

Glycine max

7117
8824

1097-1116
XP_003520128
356502644
1

Glycine max

7118
8825

998-1017
XP_003550708
356564947
1

Glycine max

7119
8826

178-197
XP_003523155
356508826
1

Glycine max

7120
8827

590-609
NP_001236309
351724988
1

Glycine max

7121
8828

XP_003529339
356521389
0.838

Glycine max

7122
8829

710-729
XP_003551421
356566402
1

Glycine max

7123
8830

XP_003518080
356498481
0.7962

Glycine max

7124
8831

pta-
161-180
XP_003549130
356561725
1

Glycine max

7125
8832

miR156b

XP_003553944
356571558
0.9452

Glycine max

7126
8833

320-339
XP_003531511
356525799
1

Glycine max

7127
8834

XP_003546792
356556969
0.7324

Glycine max

7128
8835

115-134
XP_003525436
356513468
1

Glycine max

7129
8836

XP_003550708
356564947
0.9204

Glycine max

7130
8837

XP_003522278
356507037
0.7595

Glycine max

7131
8838

106-125
XP_003520455
356503312
1

Glycine max

7132
8839

XP_003530170
356523079
0.848

Glycine max

7133
8840

47-66
XP_003550708
356564947
1

Glycine max

7134
8841

XP_003525436
356513468
0.9373

Glycine max

7135
8842

495-514
XP_003553428
356570509
1

Glycine max

7136
8843

XP_003520534
356503475
0.9081

Glycine max

7137
8844

396-415
ABW03160
157922334
1

Pisum sativum

7138
8845

XP_003546545
356556463
0.9946
lipoamide
7139
8846

ACU19644
255638677
0.9919

Glycine max

7140
8847

XP_003533815
356530492
0.9621
lipoamide
7141
8848

CAG14980
45720177
0.8916

Cicer arietinum

7142
8849

ABW03161
157922336
0.8862

Pisum sativum

7143
8850

XP_003595440
357450326
0.878

Medicago truncatula

7144
8851

XP_002314330
255761085
0.8699

Populus trichocarpa

7145

XP_003621874
357503170
0.8564

Medicago truncatula

7146
8852

XP_002267959
225432170
0.8618

Vitis vinifera

7147
8853

1563-1582
XP_002526256
255761086
1

Ricinus communis

7148

XP_002318437
255761085
0.9495

Populus trichocarpa

7149

XP_002276600
225455335
0.946

Vitis vinifera

7150
8854

CBI23029
270234399
0.946

Vitis vinifera

7151
8855

XP_002515853
255761086
0.9414

Ricinus communis

7152

XP_003530452
356523654
0.8886

Glycine max

7153
8856

XP_003525330
356513256
0.884

Glycine max

7154
8857

XP_002864245
297853636
0.8794

Arabidopsis lyrata

7155

subsp. lyrata

NP_200160
42568511
0.876

Arabidopsis thaliana

7156
8858

BAJ93177
326526000
0.8324

Hordeum vulgare

7157
8859

subsp. vulgare

393-412
XP_003520128
356502644
1

Glycine max

7158
8860

XP_003517860
356498034
0.9129

Glycine max

7159
8861

109-128
XP_003522278
356507037
1

Glycine max

7160
8862

178-197
XP_003523155
356508826
1

Glycine max

7161
8863

203-222
XP_003551276
356566105
1

Glycine max

7162
8864

XP_003538548
356540139
0.9368

Glycine max

7163
8865

XP_003601783
357463002
0.8103

Medicago truncatula

7164
8866

XP_003524894
356512372
0.7615

Glycine max

7165
8867

XP_003531195
356525159
0.7759

Glycine max

7166
8868

XP_002514915
255761086
0.7557

Ricinus communis

7167

XP_002297844
255761085
0.7471

Populus trichocarpa

7168

XP_002304680
255761085
0.7414

Populus trichocarpa

7169

XP_002271442
225425417
0.7098

Vitis vinifera

7170
8869

74-93
XP_003520455
356503312
1

Glycine max

7171
8870

370-389
XP_003553428
356570509
1

Glycine max

7172
8871

758-777
XP_003520534
356503475
1

Glycine max

7173
8872

XP_003553428
356570509
0.9081

Glycine max

7174
8873

107-126
XP_003553944
356571558
1

Glycine max

7175
8874

35-54
AAM12880
20149261
1

Helianthus annuus

7176
8875

CBI28152
270240501
0.991

Vitis vinifera

7177

XP_002284967
225430201
0.991

Vitis vinifera

7178
8876

CBI21000
270231236
0.991

Vitis vinifera

7179

CBI36254
270253379
0.9819

Vitis vinifera

7180
8877

NP_200330
145359269
0.9864

Arabidopsis thaliana

7181
8878

XP_003522628
356507751
0.9819

Glycine max

7182
8879

AEM97804
344189954
0.9864

Dimocarpus longan

7183
8880

XP_002864382
297853636
0.9864

Arabidopsis lyrata

7184

subsp. lyrata

XP_002285307
225442824
0.9819

Vitis vinifera

7185
8881

1019-1038
XP_003532399
356527605
1

Glycine max

7186
8882

XP_003525415
356513426
0.8914

Glycine max

7187
8883

113-132
ACU18105
255635506
1

Glycine max

7188
8884

755-774
XP_003518080
356498481
1

Glycine max

7189
8885

XP_003551421
356566402
0.8408

Glycine max

7190
8886

117-136
XP_003549130
356561725
1

Glycine max

7191
8887

690-709
XP_003525415
356513426
1

Glycine max

7192
8888

XP_003532399
356527605
0.9051

Glycine max

7193
8889

1067-1086
XP_003525436
356513468
1

Glycine max

7194
8890

1097-1116
XP_003520128
356502644
1

Glycine max

7195
8891

998-1017
XP_003550708
356564947
1

Glycine max

7196
8892

178-197
XP_003523155
356508826
1

Glycine max

7197
8893

467-486
XP_003610314
357480056
1

Medicago truncatula

7198
8894

XP_003549541
356562566
0.8796

Glycine max

7199
8895

XP_003519149
356500658
0.8728

Glycine max

7200
8896

XP_003540100
356543299
0.8605

Glycine max

7201
8897

XP_002278464
225445858
0.8098

Vitis vinifera

7202
8898

XP_002529385
255761086
0.8167

Ricinus communis

7203

XP_002325129
255761085
0.7852

Populus trichocarpa

7204

NP_851209
42570605
0.762

Arabidopsis thaliana

7205
8899

XP_002864540
297853636
0.7579

Arabidopsis lyrata

7206

subsp. lyrata

NP_974953
42573713
0.7565

Arabidopsis thaliana

7207
8900

710-729
XP_003551421
356566402
1

Glycine max

7208
8901

XP_003518080
356498481
0.7962

Glycine max

7209
8902

ptc-
312-334
XP_003547131
356557655
1

Glycine max

7210
8903

miRf10226-

XP_003541752
356546681
0.9148

Glycine max

7211
8904

akr
247-269
XP_003542817
356548860
1

Glycine max

7212
8905

XP_003546711
356556804
0.9141

Glycine max

7213
8906

AET02361
357515192
0.7607

Medicago truncatula

7214
8907

131-153
XP_003523607

1

Glycine max

7215

XP_003525906

0.9439

Glycine max

7216

106-128
XP_003549610
356562705
1

Glycine max

7217
8908

XP_003529657
356522038
0.8762

Glycine max

7218
8909

161-183
XP_003525906
356514424
1

Glycine max

7219
8910

XP_003523607
356509746
0.9381

Glycine max

7220
8911

ptc-
123-143
XP_003543825
356550908
1

Glycine max

7221
8912

miRf10271-

XP_003556814
356577399
0.813

Glycine max

7222
8913

akr
423-443
XP_002325684
255761085
1

Populus trichocarpa

7223

XP_002319934
255761085
0.9342

Populus trichocarpa

7224

ACU14088
255627486
0.8202

Glycine max

7225
8914

XP_003543368
356549979
0.8114

Glycine max

7226
8915

NP_001237648
351726723
0.7982

Glycine max

7227
8916

ACU20677
255640786
0.7807

Glycine max

7228
8917

XP_002284361
225461286
0.7719

Vitis vinifera

7229
8918

ADU05416
315364829
0.7851

Citrullus lanatus

7230
8919

XP_002265183
225465748
0.75

Vitis vinifera

7231
8920

XP_002513246
255761086
0.807

Ricinus communis

7232

544-564
ACU20677
255640786
1

Glycine max

7233
8921

P26291

0.7099

Pisum sativum

7234

XP_003597086
357453610
0.7023

Medicago truncatula

7235
8922

304-324
XP_003541823
356546825
1

Glycine max

7236
8923

288-308
XP_003518627
356499601
1

Glycine max

7237
8924

XP_003542153
356547506
0.9478

Glycine max

7238
8925

ADN33938
307136081
0.7937

Cucumis melo

7239
8926

subsp. melo

XP_002518919
255761086
0.7755

Ricinus communis

7240

XP_002279642
225426567
0.7755

Vitis vinifera

7241
8927

XP_002870592
297853636
0.7664

Arabidopsis lyrata

7242

subsp. lyrata

NP_199024
30693991
0.7642

Arabidopsis thaliana

7243
8928

AAM63843
21405504
0.7596

Arabidopsis thaliana

7244
8929

XP_002299422
255761085
0.7528

Populus trichocarpa

7245

XP_002303695
255761085
0.7574

Populus trichocarpa

7246

123-143
XP_003543825
356550908
1

Glycine max

7247
8930

460-480
XP_003542153
356547506
1

Glycine max

7248
8931

XP_003518627
356499601
0.9436

Glycine max

7249
8932

838-858
XP_003526354
356515330
1

Glycine max

7250
8933

XP_003523913
356510372
0.9333

Glycine max

7251
8934

72-92
XP_003535315
356533526
1

Glycine max

7252
8935

XP_003555178
356574075
0.9462

Glycine max

7253
8936

XP_003591226
357441896
0.7849

Medicago truncatula

7254
8937

XP_002512536
255761086
0.7465

Ricinus communis

7255

CBI39621
270257428
0.7465

Vitis vinifera

7256
8938

CAP59645
163913883
0.7558

Vitis vinifera

7257
8939

XP_002277312
225450534
0.7496

Vitis vinifera

7258
8940

XP_002280462
225432056
0.7404

Vitis vinifera

7259
8941

CAP59646
163913885
0.7512

Vitis vinifera

7260
8942

CAN63178
123711273
0.7373

Vitis vinifera

7261
8943

157-177
XP_003594856
357449158
1

Medicago truncatula

7262
8944

XP_003533661
356530178
0.913

Glycine max

7263
8945

ACU18911
255637160
0.9091

Glycine max

7264
8946

ACU19698
255638787
0.9051

Glycine max

7265
8947

XP_002273965
225423594
0.83

Vitis vinifera

7266
8948

NP_001058303
115469407
0.7984

Oryza sativa

7267
8949

Japonica Group

XP_002528147
255761086
0.8063

Ricinus communis

7268

AEL99129
343172851
0.8142

Silene latifolia

7269
8950

AEL99130
343172853
0.8103

Silene latifolia

7270
8951

XP_002438809
255761094
0.7787

Sorghum bicolor

7271

613-633
XP_003541563
356546291
1

Glycine max

7272
8952

XP_003545791
356554924
0.805

Glycine max

7273
8953

904-924
XP_003556814
356577399
1

Glycine max

7274
8954

2015-2035
XP_003538988
356541033
1

Glycine max

7275
8955

XP_003607189
357473808
0.7407

Medicago truncatula

7276
8956

XP_003604038
357467506
0.7035

Medicago truncatula

7277
8957

841-861
XP_003523913
356510372
1

Glycine max

7278
8958

XP_003526354
356515330
0.9333

Glycine max

7279
8959

925-945
XP_003545791
356554924
1

Glycine max

7280
8960

XP_003541563
356546291
0.8662

Glycine max

7281
8961

ptc-
371-391
XP_003518621
356499589
1

Glycine max

7282
8962

miRf10734-

XP_003529232
356521172
0.9575

Glycine max

7283
8963

akr

ABI48270
113911567
0.8826

Lotus japonicus

7284
8964

XP_003525213
356513021
0.8512

Glycine max

7285
8965

XP_003530935
356524637
0.8502

Glycine max

7286
8966

XP_003631014
357521450
0.836

Medicago truncatula

7287
8967

XP_002285117
225424439
0.8117

Vitis vinifera

7288
8968

ACE63259
190148352
0.8067

Betula pendula

7289
8969

ABI48271
113911569
0.8259

Lotus japonicus

7290
8970

XP_002314765
255761085
0.7783

Populus trichocarpa

7291

1151-1171
XP_003520774
356503973
1

Glycine max

7292
8971

1761-1781
XP_003523576
356509683
1

Glycine max

7293

XP_003527692
356518039
0.8899

Glycine max

7294

1929-1949
XP_003527692
356518039
1

Glycine max

7295

XP_003523576
356509683
0.8906

Glycine max

7296

1230-1250
XP_003538849
356540752
1

Glycine max

7297
8972

XP_003520774
356503973
0.7213

Glycine max

7298
8973

ptc-
161-180
XP_003549130
356561725
1

Glycine max

7299
8974

miRf10985-

XP_003553944
356571558
0.9452

Glycine max

7300
8975

akr
1659-1678
AAF67341
7682676
1

Vigna radiata

7301
8976

ACF22882
193850556
0.9362

Glycine max

7302

XP_003546457
356556285
0.9334

Glycine max

7303
8977

XP_003550633
356564793
0.8239

Glycine max

7304
8978

CAA09457
3641864
0.8017

Cicer arietinum

7305
8979

CAA06309
14274980
0.7961

Cicer arietinum

7306
8980

CAA09467
3860419
0.8058

Lupinus angustifolius

7307
8981

XP_002514108
255761086
0.7878

Ricinus communis

7308

XP_003595162
357449770
0.7906

Medicago truncatula

7309
8982

XP_002308268
255761085
0.7684

Populus trichocarpa

7310

235-254
BAF31130
114213453
1

Vicia faba

7311
8983

P48488

0.9751

Medicago sativa

7312

subsp. × varia

ACJ84258
217071795
0.972

Medicago truncatula

7313
8984

XP_003532976
356528780
0.9346

Glycine max

7314
8985

XP_003525372
356513340
0.9283

Glycine max

7315
8986

ACU20069
255639548
0.9252

Glycine max

7316
8987

XP_002509868
255761086
0.9034

Ricinus communis

7317

XP_002298008
255761085
0.8972

Populus trichocarpa

7318

XP_002277816
225426133
0.9034

Vitis vinifera

7319
8988

NP_176587
145337150
0.8442

Arabidopsis thaliana

7320
8989

235-254
XP_003551188
356565928
1

Glycine max

7321
8990

XP_003538544
356540131
0.9106

Glycine max

7322
8991

ACU18328
255635963
0.9083

Glycine max

7323
8992

XP_003601767
357462970
0.7064

Medicago truncatula

7324
8993

495-514
XP_003553428
356570509
1

Glycine max

7325
8994

XP_003520534
356503475
0.9081

Glycine max

7326
8995

51-70
XP_003545057
356553424
1

Glycine max

7327
8996

XP_003519693
356501762
0.9036

Glycine max

7328
8997

XP_002312804
255761085
0.7267

Populus trichocarpa

7329

XP_002279611
225428277
0.7199

Vitis vinifera

7330
8998

167-186
XP_003538544
356540131
1

Glycine max

7331
8999

XP_003551188
356565928
0.9097

Glycine max

7332
9000

109-128
XP_003522278
356507037
1

Glycine max

7333
9001

XP_003525436
356513468
0.7319

Glycine max

7334
9002

XP_003550708
356564947
0.7072

Glycine max

7335
9003

393-412
XP_003520128
356502644
1

Glycine max

7336
9004

XP_003517860
356498034
0.9129

Glycine max

7337
9005

217-236
AES84797
339649045
1

Medicago truncatula

7338
9006

XP_003518611
356499568
0.8041

Glycine max

7339
9007

XP_003529219
356521146
0.7947

Glycine max

7340
9008

XP_003525231
356513057
0.7665

Glycine max

7341
9009

XP_003630941
357521304
0.7834

Medicago truncatula

7342
9010

XP_003530877
356524520
0.7552

Glycine max

7343
9011

XP_002525995
255761086
0.7269

Ricinus communis

7344

XP_002315857
255761085
0.7213

Populus trichocarpa

7345

ABK94575
118485436
0.7213

Populus trichocarpa

7346
9012

XP_002311530
255761085
0.7175

Populus trichocarpa

7347

398-417
XP_003588450
357436348
1

Medicago truncatula

7348
9013

XP_003526513
356515652
0.8496

Glycine max

7349
9014

XP_003522729
356507956
0.8453

Glycine max

7350
9015

XP_003603665
357466760
0.839

Medicago truncatula

7351
9016

ACU21356
255642182
0.8432

Glycine max

7352
9017

XP_003550117
356563738
0.8305

Glycine max

7353
9018

CAD92450
31455392
0.7479

Brassica napus

7354
9019

ADB92670
284519839
0.75

Populus tremula ×
7355
9020

Populus alba

XP_002510013
255761086
0.7373

Ricinus communis

7356

XP_002301129
255761085
0.7373

Populus trichocarpa

7357

370-389
XP_003553428
356570509
1

Glycine max

7358
9021

758-777
XP_003520534
356503475
1

Glycine max

7359
9022

XP_003553428
356570509
0.9081

Glycine max

7360
9023

236-255
XP_003551188
356565928
1

Glycine max

7361
9024

35-54
AAM12880
20149261
1

Helianthus annuus

7362
9025

CBI28152
270240501
0.991

Vitis vinifera

7363

XP_002284967
225430201
0.991

Vitis vinifera

7364
9026

CBI21000
270231236
0.991

Vitis vinifera

7365

CBI36254
270253379
0.9819

Vitis vinifera

7366
9027

NP_200330
145359269
0.9864

Arabidopsis thaliana

7367
9028

XP_003522628
356507751
0.9819

Glycine max

7368
9029

AEM97804
344189954
0.9864

Dimocarpus longan

7369
9030

XP_002864382
297853636
0.9864

Arabidopsis lyrata

7370

subsp. lyrata

XP_002285307
225442824
0.9819

Vitis vinifera

7371
9031

755-774
XP_003518080
356498481
1

Glycine max

7372
9032

XP_003551421
356566402
0.8408

Glycine max

7373
9033

56-75
XP_003522398
356507283
1

Glycine max

7374
9034

XP_003526192
356515005
0.9095

Glycine max

7375
9035

XP_003526191
356515003
0.9128

Glycine max

7376
9036

XP_003527777
356518217
0.7966

Glycine max

7377
9037

113-132
XP_003538544
356540131
1

Glycine max

7378
9038

1097-1116
XP_003520128
356502644
1

Glycine max

7379
9039

1482-1501
XP_003546504
356556379
1

Glycine max

7380
9040

XP_003550617
356564761
0.7923

Glycine max

7381
9041

XP_003542359
356547932
0.7889

Glycine max

7382
9042

XP_002279041
225444747
0.7337

Vitis vinifera

7383
9043

XP_002870435
297853636
0.7119

Arabidopsis lyrata

7384

subsp. lyrata

XP_002516284
255761086
0.7102

Ricinus communis

7385

NP_568528
22327353
0.7018

Arabidopsis thaliana

7386
9044

XP_002326282
255761085
0.7002

Populus trichocarpa

7387

ptc-
264-283
XP_003518840
356500034
1

Glycine max

7388
9045

miRf11315-

XP_003529395
356521503
0.9171

Glycine max

7389
9046

akr

XP_003607985
357475398
0.8275

Medicago truncatula

7390
9047

XP_002518769
255761086
0.7579

Ricinus communis

7391

XP_002313117
255761085
0.7496

Populus trichocarpa

7392

AAQ90244
37223341
0.7446

Solanum lycopersicum

7393
9048

NP_001234399
350534489
0.7446

Solanum lycopersicum

7394
9049

NP_188555
30685246
0.7247

Arabidopsis thaliana

7395
9050

XP_002883169
297853636
0.7164

Arabidopsis lyrata

7396

subsp. lyrata

CBI32416
270245997
0.7313

Vitis vinifera

7397
9051

324-343
XP_003550774
356565082
1

Glycine max

7398
9052

XP_003525472
356513541
0.8489

Glycine max

7399
9053

324-343
XP_003550774
356565082
1

Glycine max

7400
9054

680-699
XP_003554964
356573636
1

Glycine max

7401
9055

ptc-
404-426
XP_003612685
357484794
1

Medicago truncatula

7402
9056

miRf11757-

XP_003516858
356495999
0.8914

Glycine max

7403
9057

akr

XP_003534304
356531476
0.8801

Glycine max

7404
9058

BAH03477
218744535
0.8015

Nicotiana tabacum

7405
9059

XP_002519001
255761086
0.824

Ricinus communis

7406

XP_002303454
255761085
0.8127

Populus trichocarpa

7407

ABK93338
118482845
0.8052

Populus trichocarpa

7408
9060

XP_002326571
255761085
0.8015

Populus trichocarpa

7409

XP_002274060
225427054
0.764

Vitis vinifera

7410
9061

XP_002890966
297853636
0.7603

Arabidopsis lyrata

7411

subsp. lyrata

ath-
164-184
XP_003549130
356561725
1

Glycine max

7412
9062

miR157a

XP_003553944
356571558
0.9452

Glycine max

7413
9063

238-258
XP_003551188
356565928
1

Glycine max

7414
9064

XP_003538544
356540131
0.9106

Glycine max

7415
9065

ACU18328
255635963
0.9083

Glycine max

7416
9066

XP_003601767
357462970
0.7064

Medicago truncatula

7417
9067

1129-1149
XP_003525415
356513426
1

Glycine max

7418
9068

XP_003532399
356527605
0.9051

Glycine max

7419
9069

898-918
XP_003540473
356544059
1

Glycine max

7420
9070

XP_003543233
356549706
0.9305

Glycine max

7421
9071

ACU24116
255647298
0.9251

Glycine max

7422
9072

118-138
XP_003525436
356513468
1

Glycine max

7423
9073

XP_003550708
356564947
0.9204

Glycine max

7424
9074

XP_003522278
356507037
0.7595

Glycine max

7425
9075

77-97
XP_003520455
356503312
1

Glycine max

7426
9076

XP_003530170
356523079
0.848

Glycine max

7427
9077

498-518
XP_003553428
356570509
1

Glycine max

7428
9078

XP_003520534
356503475
0.9081

Glycine max

7429
9079

50-70
XP_003550708
356564947
1

Glycine max

7430
9080

XP_003525436
356513468
0.9373

Glycine max

7431
9081

854-875
XP_003555667
356575073
1

Glycine max

7432
9082

170-190
XP_003538544
356540131
1

Glycine max

7433
9083

XP_003551188
356565928
0.9097

Glycine max

7434
9084

593-613
NP_001236309
351724988
1

Glycine max

7435
9085

XP_003529339
356521389
0.838

Glycine max

7436
9086

144-164
XP_002275728
225446415
1

Vitis vinifera

7437
9087

AAY16440
62856978
0.7396

Betula platyphylla

7438
9088

112-132
XP_003522278
356507037
1

Glycine max

7439
9089

396-416
XP_003520128
356502644
1

Glycine max

7440
9090

XP_003517860
356498034
0.9129

Glycine max

7441
9091

181-201
XP_003523155
356508826
1

Glycine max

7442
9092

593-613
NP_001236309
351724988
1

Glycine max

7443
9093

116-136
ACU18105
255635506
1

Glycine max

7444
9094

758-778
XP_003518080
356498481
1

Glycine max

7445
9095

XP_003551421
356566402
0.8408

Glycine max

7446
9096

120-140
XP_003549130
356561725
1

Glycine max

7447

693-713
XP_003525415
356513426
1

Glycine max

7448
9097

737-757
XP_003553428
356570509
1

Glycine max

7449
9098

116-136
XP_003538544
356540131
1

Glycine max

7450
9099

818-838
XP_003553944
356571558
1

Glycine max

7451
9100

238-258
XP_003551188
356565928
1

Glycine max

7452
9101

1070-1090
XP_003525436
356513468
1

Glycine max

7453
9102

109-129
XP_003520455
356503312
1

Glycine max

7454
9103

404-424
XP_003526029
356514674
1

Glycine max

7455
9104

XP_003540122
356543345
0.8919

Glycine max

7456
9105

181-201
XP_003523155
356508826
1

Glycine max

7457
9106

713-733
XP_003551421
356566402
1

Glycine max

7458
9107

XP_003518080
356498481
0.7962

Glycine max

7459
9108

sbi-
305-325
XP_003541823
356546825
1

Glycine max

7460
9109

miR159a
305-325
XP_003541823
356546825
1

Glycine max

7461
9110

289-309
XP_003518627
356499601
1

Glycine max

7462
9111

XP_003542153
356547506
0.9478

Glycine max

7463
9112

ADN33938
307136081
0.7937

Cucumis melo

7464
9113

subsp. melo

XP_002518919
255761086
0.7755

Ricinus communis

7465

XP_002279642
225426567
0.7755

Vitis vinifera

7466
9114

XP_002870592
297853636
0.7664

Arabidopsis lyrata

7467

subsp. lyrata

NP_199024
30693991
0.7642

Arabidopsis thaliana

7468
9115

AAM63843
21405504
0.7596

Arabidopsis thaliana

7469
9116

XP_002299422
255761085
0.7528

Populus trichocarpa

7470

XP_002303695
255761085
0.7574

Populus trichocarpa

7471

124-144
XP_003543825
356550908
1

Glycine max

7472
9117

XP_003556814
356577399
0.813

Glycine max

7473
9118

461-481
XP_003542153
356547506
1

Glycine max

7474
9119

XP_003518627
356499601
0.9436

Glycine max

7475
9120

839-859
XP_003526354
356515330
1

Glycine max

7476
9121

XP_003523913
356510372
0.9333

Glycine max

7477
9122

73-93
XP_003535315
356533526
1

Glycine max

7478
9123

XP_003555178
356574075
0.9462

Glycine max

7479
9124

XP_003591226
357441896
0.7849

Medicago truncatula

7480
9125

XP_002512536
255761086
0.7465

Ricinus communis

7481

CBI39621
270257428
0.7465

Vitis vinifera

7482
9126

CAP59645
163913883
0.7558

Vitis vinifera

7483
9127

XP_002277312
225450534
0.7496

Vitis vinifera

7484
9128

XP_002280462
225432056
0.7404

Vitis vinifera

7485
9129

CAP59646
163913885
0.7512

Vitis vinifera

7486
9130

CAN63178
123711273
0.7373

Vitis vinifera

7487
9131

614-634
XP_003541563
356546291
1

Glycine max

7488
9132

XP_003545791
356554924
0.805

Glycine max

7489
9133

905-925
XP_003556814
356577399
1

Glycine max

7490
9134

XP_003543825
356550908
0.8659

Glycine max

7491
9135

smo-
164-184
XP_003549130
356561725
1

Glycine max

7492
9136

miR156b

XP_003553944
356571558
0.9452

Glycine max

7493
9137

238-258
XP_003551188
356565928
1

Glycine max

7494
9138

XP_003538544
356540131
0.9106

Glycine max

7495
9139

ACU18328
255635963
0.9083

Glycine max

7496
9140

XP_003601767
357462970
0.7064

Medicago truncatula

7497
9141

1129-1149
XP_003525415
356513426
1

Glycine max

7498
9142

XP_003532399
356527605
0.9051

Glycine max

7499
9143

118-138
XP_003525436
356513468
1

Glycine max

7500
9144

XP_003550708
356564947
0.9204

Glycine max

7501
9145

XP_003522278
356507037
0.7595

Glycine max

7502
9146

77-97
XP_003520455
356503312
1

Glycine max

7503
9147

XP_003530170
356523079
0.848

Glycine max

7504
9148

50-70
XP_003550708
356564947
1

Glycine max

7505
9149

XP_003525436
356513468
0.9373

Glycine max

7506
9150

498-518
XP_003553428
356570509
1

Glycine max

7507
9151

XP_003520534
356503475
0.9081

Glycine max

7508
9152

170-190
XP_003538544
356540131
1

Glycine max

7509
9153

XP_003551188
356565928
0.9097

Glycine max

7510
9154

593-613
NP_001236309
351724988
1

Glycine max

7511
9155

XP_003529339
356521389
0.838

Glycine max

7512
9156

475-495
XP_003528960
356520620
1

Glycine max

7513
9157

NP_001235425
351721650
0.9279

Glycine max

7514
9158

144-164
XP_002275728
225446415
1

Vitis vinifera

7515
9159

AAY16440
62856978
0.7396

Betula platyphylla

7516
9160

112-132
XP_003522278
356507037
1

Glycine max

7517
9161

396-416
XP_003520128
356502644
1

Glycine max

7518
9162

XP_003517860
356498034
0.9129

Glycine max

7519
9163

243-263
XP_003540122
356543345
1

Glycine max

7520
9164

XP_003526029
356514674
0.8684

Glycine max

7521
9165

181-201
XP_003523155
356508826
1

Glycine max

7522
9166

77-97
XP_003520455
356503312
1

Glycine max

7523
9167

373-393
XP_003553428
356570509
1

Glycine max

7524
9168

761-781
XP_003520534
356503475
1

Glycine max

7525
9169

XP_003553428
356570509
0.9081

Glycine max

7526
9170

110-130
XP_003553944
356571558
1

Glycine max

7527
9171

239-259
XP_003551188
356565928
1

Glycine max

7528
9172

1022-1042
XP_003532399
356527605
1

Glycine max

7529
9173

XP_003525415
356513426
0.8914

Glycine max

7530
9174

593-613
NP_001236309
351724988
1

Glycine max

7531
9175

116-136
ACU18105
255635506
1

Glycine max

7532
9176

758-778
XP_003518080
356498481
1

Glycine max

7533
9177

XP_003551421
356566402
0.8408

Glycine max

7534
9178

1231-1251
XP_003525415
356513426
1

Glycine max

7535
9179

20-40
XP_003549130
356561725
1

Glycine max

7536
9180

1070-1090
XP_003525436
356513468
1

Glycine max

7537
9181

404-424
XP_003526029
356514674
1

Glycine max

7538
9182

XP_003540122
356543345
0.8919

Glycine max

7539
9183

185-205
XP_003538544
356540131
1

Glycine max

7540
9184

713-733
XP_003551421
356566402
1

Glycine max

7541
9185

XP_003518080
356498481
0.7962

Glycine max

7542
9186

osa-
32-52
AAM97011
22531013
1

Arabidopsis thaliana

7543
9187

miRf10839-

NP_563815
145335272
0.9929

Arabidopsis thaliana

7544
9188

akr

XP_002892455
297853636
0.9786

Arabidopsis lyrata

7545

subsp. lyrata

BAJ33638
312281544
0.9429

Thellungiella halophila

7546
9189

XP_002523852
255761086
0.7714

Ricinus communis

7547

AEL99169
343172931
0.75

Silene latifolia

7548
9190

XP_002313603
255761085
0.7429

Populus trichocarpa

7549

ACJ86143
217075565
0.7214

Medicago truncatula

7550
9191

ADI45844
297525844
0.7429

Silene vulgaris

7551
9192

XP_002328107
255761085
0.75

Populus trichocarpa

7552

ptc-
35-54
ACJ37435
212717187
1

Glycine max

7553
9193

miRf10300-

ACU23160
255645326
0.9948

Glycine max

7554
9194

akr

ACJ37436
212717189
0.933

Glycine max

7555
9195

537-556
NP_001235206
351722714
1

Glycine max

7556
9196

NP_001236569
351725110
0.9581

Glycine max

7557
9197

XP_002866588
297853636
0.7126

Arabidopsis lyrata

7558

subsp. lyrata

XP_002309915
255761085
0.7665

Populus trichocarpa

7559

NP_201209
145359627
0.7066

Arabidopsis thaliana

7560
9198

ABK96256
118488893
0.7605

Populus trichocarpa ×
7561
9199

Populus deltoides

XP_002526638
255761086
0.7485

Ricinus communis

7562

XP_002306249
255761085
0.7784

Populus trichocarpa

7563

2WSC_N

0.7844

Phaseolus vulgaris

7564

BAJ33864
312281996
0.7066

Thellungiella halophila

7565
9200

39-58
XP_002283864
225429937
1

Vitis vinifera

7566
9201

ptc-
318-339
BAG09382
167961874
1

Glycine max

7567
9202

miRf10619-

NP_001238412
351726609
0.9838

Glycine max

7568
9203

akr

ACU19205
255637765
0.9704

Glycine max

7569
9204

CAN67413
123711204
0.9272

Vitis vinifera

7570
9205

ACD93720
223987377
0.903

Mikania micrantha

7571
9206

XP_002862992
297853636
0.9191

Arabidopsis lyrata

7572

subsp. lyrata

XP_002277249
225462095
0.9299

Vitis vinifera

7573
9207

XP_002317470
255761085
0.9218

Populus trichocarpa

7574

1803516A

0.9191

Lens culinaris

7575

XP_002519658
255761086
0.9084

Ricinus communis

7576

32-53
ACU18791
255636916
1

Glycine max

7577
9208

1512-1532
ACQ44234
228485370
1

Glycine max

7578
9209

55-75
XP_002512977
255761086
1

Ricinus communis

7579

ptc-
418-438
ACU17540
255634349
1

Glycine max

7580
9210

miRf11847-

akr

ath-
293-314
ACU18306
255635917
1

Glycine max

7581
9211

miRf10701-
918-939
NP_001237102
351725712
1

Glycine max

7582
9212

akr

XP_002284425
225445231
0.7562

Vitis vinifera

7583
9213

348-369
NP_001236604
351726125
1

Glycine max

7584
9214

1178-1199
ACU21144
255641746
1

Glycine max

7585
9215

XP_002277008
225444658
0.7859

Vitis vinifera

7586
9216

XP_002276983
225444660
0.7859

Vitis vinifera

7587
9217

CAB75429
6996559
0.7204

Nicotiana plumbaginifolia

7588
9218

XP_002331184
255761085
0.733

Populus trichocarpa

7589

XP_002529199
255761086
0.7355

Ricinus communis

7590

XP_002516242
255761086
0.7179

Ricinus communis

7591

XP_002270823
225442060
0.7254

Vitis vinifera

7592
9219

CBI35841
270253379
0.7179

Vitis vinifera

7593

CAN65009
147797980
0.7229

Vitis vinifera

7594
9220

osa-
321-339
ABB02162
77744234
1

Medicago sativa

7595
9221

miRf11595-

ABB02161
77744232
0.975

Medicago sativa

7596
9222

akr

ACJ85732
217074743
0.9711

Medicago truncatula

7597
9223

AEO21428
346229108
0.8227

Glycine max

7598
9224

ABC59101
84514184
0.8073

Medicago truncatula

7599
9225

ABC68398
85001688
0.7842

Glycine max

7600
9226

ABS53040
153869430
0.8073

Leucaena leucocephala

7601
9227

AAT39511
47933889
0.7553

Camptotheca acuminata

7602
9228

XP_002327769
255761085
0.7572

Populus trichocarpa

7603

XP_002327770
255761085
0.7437

Populus trichocarpa

7604

107-125
NP_001236740
351722666
1

Glycine max

7605
9229

56-74
CAE02645
34495198
1

Lotus japonicus

7606
9230

CAA65585
2347053
0.7513

Vitis vinifera

7607
9231

AAP36992
46371994
0.753

Cucumis sativus

7608
9232

BAE71301
84468435
0.7598

Trifolium pratense

7609
9233

XP_002269030
225427781
0.7445

Vitis vinifera

7610
9234

BAE71251
84468335
0.7581

Trifolium pratense

7611
9235

BAG68575
195976672
0.7496

Prunus persica

7612
9236

BAD06581
40645471
0.7394

Nicotiana tabacum

7613
9237

CAN65288
147782233
0.7291

Vitis vinifera

7614
9238

CAB64599
6646839
0.7359

Datura stramonium

7615
9239

311-329
ACU18654
255636632
1

Glycine max

7616
9240

515-533
NP_001235161
351721419
1

Glycine max

7617
9241

BAB86923
19911192
0.711

Vigna angularis

7618
9242

22-40
NP_001237655
351726929
1

Glycine max

7619
9243

421-439
ACU22898
255644792
1

Glycine max

7620
9244

osa-
407-428
NP_001237033
351723724
1

Glycine max

7621
9245

miRf11013-
32-53
ABY84658
166203235
1

Glycine max

7622
9246

akr

NP_001236902
351727360
0.9715

Glycine max

7623
9247

AAL32033
18158618
0.7967

Retama raetam

7624
9248

597-618
ACU23333
255645678
1

Glycine max

7625
9249

29-50
NP_001236902
351727360
1

Glycine max

7626
9250

ABY84658
166203235
0.9696

Glycine max

7627
9251

ptc-
679-698
NP_001238384
351725780
1

Glycine max

7628
9252

miRf10148-
547-566
XP_002283799
225435835
1

Vitis vinifera

7629
9253

akr

CAN72395
147774368
0.9606

Vitis vinifera

7630

ACU20767
255640974
0.9462

Glycine max

7631
9254

XP_002304844
255761085
1.0896

Populus trichocarpa

7632

CBI16575
270227042
0.9176

Vitis vinifera

7633

XP_002523383
255761086
0.8746

Ricinus communis

7634

XP_002299088
255761085
0.8853

Populus trichocarpa

7635

NP_850182
42570348
0.81

Arabidopsis thaliana

7636
9255

NP_565736
42569548
0.8065

Arabidopsis thaliana

7637
9256

XP_002862885
297853636
0.8029

Arabidopsis lyrata

7638

subsp. lyrata

135-154
ACU17996
255635284
1

Glycine max

7639
9257

ACU23482
255645988
0.8725

Glycine max

7640
9258

XP_002307595
255761085
0.8406

Populus trichocarpa

7641

XP_002524761
255761086
0.8319

Ricinus communis

7642

XP_002300832
255761085
0.8261

Populus trichocarpa

7643

XP_002279156
225437057
0.8029

Vitis vinifera

7644
9259

XP_002878504
297853636
0.7072

Arabidopsis lyrata

7645

subsp. lyrata

141-160
XP_002283799
225435835
1

Vitis vinifera

7646
9260

367-386
ACU17970
255635230
1

Glycine max

7647
9261

ACU18145
255635589
0.9494

Glycine max

7648
9262

XP_002510492
255761086
0.7946

Ricinus communis

7649

XP_002278539
225458064
0.7798

Vitis vinifera

7650
9263

XP_002306908
255761085
0.7619

Populus trichocarpa

7651

ABL10371
118723367
0.75

Medicago truncatula

7652
9264

XP_002301996
255761085
0.7738

Populus trichocarpa

7653

NP_568605
145358761
0.7024

Arabidopsis thaliana

7654
9265

110-129
ACU17996
255635284
1

Glycine max

7655
9266

zma-
122-140
ACU21375
255642220
1

Glycine max

7656
9267

miR482-5p
128-146
XP_002314999
255761085
1

Populus trichocarpa

7657

XP_002312287
255761085
0.9432

Populus trichocarpa

7658

XP_002285502
225424638
0.8428

Vitis vinifera

7659
9268

XP_002520520
255761086
0.8341

Ricinus communis

7660

XP_002876891
297853636
0.8035

Arabidopsis lyrata

7661

subsp. lyrata

NP_178465
30678070
0.7948

Arabidopsis thaliana

7662
9269

NP_001149451
226506925
0.7773

Zea mays

7663
9270

XP_002875224
297853636
0.7904

Arabidopsis lyrata

7664

subsp. lyrata

NP_001054237
115461273
0.7686

Oryza sativa

7665
9271

Japonica Group

CAH68184
90399185
0.7642

Oryza sativa

7666
9272

Indica Group

745-763
NP_001235027
351724922
1

Glycine max

7667
9273

NP_001238461
351728042
0.9389

Glycine max

7668
9274

NP_001235888
351727642
0.9389

Glycine max

7669
9275

NP_001235511
351724120
0.9313

Glycine max

7670
9276

ADD11814
289586041
0.8244

Cajanus cajan

7671
9277

AAC49369
1420884
0.7939

Phaseolus vulgaris

7672
9278

40-58
ACU20629
255640688
1

Glycine max

7673
9279

27-45
ACU20555
255640539
1

Glycine max

7674
9280

19-37
ACU23548
255646123
1

Glycine max

7675
9281

Q96452

0.9922

Glycine max

7676

NP_001235679
351721598
1

Glycine max

7677
9282

ACU19187
255637728
0.9457

Glycine max

7678
9283

ACU17765
255634808
0.8876

Glycine max

7679
9284

XP_002523376
255761086
0.8411

Ricinus communis

7680

ACQ45020
228552591
0.845

Cicer arietinum

7681
9285

XP_002285427
225461653
0.8256

Vitis vinifera

7682
9286

P42654

0.8372

Vicia faba

7683

XP_002316863
255761085
0.8333

Populus trichocarpa

7684

59-77
ACU24228
255647528
1

Glycine max

7685
9287

ACU18882
255637100
0.7652

Glycine max

7686
9288

XP_002276186
225429425
0.7652

Vitis vinifera

7687
9289

XP_002516481
255761086
0.7391

Ricinus communis

7688

XP_002324746
255761085
0.7304

Populus trichocarpa

7689

NP_565612
145360329
0.7217

Arabidopsis thaliana

7690
9290

XP_002880724
297853636
0.7275

Arabidopsis lyrata

7691

subsp. lyrata

XP_002281479
225442372
0.7362

Vitis vinifera

7692
9291

ABB86253
82621129
0.7014

Solanum tuberosum

7693
9292

CBI35995
270253379
0.7362

Vitis vinifera

7694

296-314
NP_001237978
351721473
1

Glycine max

7695
9293

59-77
ACU18882
255637100
1

Glycine max

7696
9294

ACU24228
255647528
0.9209

Glycine max

7697
9295

NP_001031418
79323070
0.723

Arabidopsis thaliana

7698
9296

204-222
ACU20859
255641163
1

Glycine max

7699
9297

91-109
ACU15870
255631011
1

Glycine max

7700
9298

390-408
CAI43251
57283984
1

Phaseolus vulgaris

7701
9299

var. nanus

ABA86966
77540215
0.937

Glycine max

7702
9300

NP_001237472
351721637
0.937

Glycine max

7703
9301

ACU23435
255645890
0.9213

Glycine max

7704
9302

XP_002283671
225434934
0.8228

Vitis vinifera

7705
9303

ACJ11723
211906459
0.8386

Gossypium hirsutum

7706
9304

ABA46792
76573374
0.8228

Solanum tuberosum

7707
9305

XP_002299871
255761085
0.815

Populus trichocarpa

7708

CAN67342
147772559
0.815

Vitis vinifera

7709

XP_002876302
297853636
0.811

Arabidopsis lyrata

7710

subsp. lyrata

61-79
ACU17423
255634119
1

Glycine max

7711
9306

140-158
ABA86966
77540215
1

Glycine max

7712
9307

CAI43251
57283984
0.9407

Phaseolus vulgaris

7713
9308

var. nanus

XP_002283693
225449540
0.8261

Vitis vinifera

7714
9309

309-327
AAV87173
56404220
1

Phaseolus vulgaris

7715
9310

BAD97829
63002633
0.7487

Prunus persica

7716
9311

ptc-
79-98
ACU20325
255640067
1

Glycine max

7717
9312

miRf10522-
321-340
ADM32504
304421409
1

Glycine max

7718
9313

akr

CAD12837
18075959
0.8679

Lupinus luteus

7719
9314

CBI27290
270239516
0.7896

Vitis vinifera

7720

XP_002316086
255761085
0.7847

Populus trichocarpa

7721

XP_002512077
255761086
0.7765

Ricinus communis

7722

CAD12836
18075957
0.7471

Lupinus luteus

7723
9315

XP_002311341
255761085
0.7553

Populus trichocarpa

7724

NP_172830
145335664
0.7259

Arabidopsis thaliana

7725
9316

XP_002892769
297853636
0.7243

Arabidopsis lyrata

7726

subsp. lyrata

1811-1830
NP_001237605
351725462
1

Glycine max

7727
9317

NP_001235120
351727636
0.7738

Glycine max

7728
9318

osa-
663-683
ACU20209
255639831
1

Glycine max

7729
9319

miRf10362-
309-329
XP_002531192
255761086
1

Ricinus communis

7730

akr

XP_002298817
255761085
0.7018

Populus trichocarpa

7731

415-435
NP_001238595
351724492
1

Glycine max

7732
9320

ath-
261-280
ACU23703
255646448
1

Glycine max

7733
9321

miRf10702-
328-347
ABD28727
49405947
1

Medicago truncatula

7734

akr

NP_001236252
351723348
0.9592

Glycine max

7735
9322

NP_001236882
351726775
0.9456

Glycine max

7736
9323

CAG14986
45720189
0.9184

Cicer arietinum

7737
9324

XP_002268544
225442984
0.8844

Vitis vinifera

7738
9325

XP_002517623
255761086
0.8912

Ricinus communis

7739

XP_002298252
255761085
0.8707

Populus trichocarpa

7740

NP_172989
145335736
0.8639

Arabidopsis thaliana

7741
9326

EEC76567
54362548
0.8571

Oryza sativa

7742

Indica Group

BAJ92153
326514005
0.8435

Hordeum vulgare

7743
9327

subsp. vulgare

690-709
ACU24052
255647167
1

Glycine max

7744
9328

NP_001237955
351720763
0.8561

Glycine max

7745
9329

ath-
343-362
NP_001234975
351723428
1

Glycine max

7746
9330

miRf10148-

XP_002314377
255761085
0.8174

Populus trichocarpa

7747

akr

XP_002270067
225431754
0.8119

Vitis vinifera

7748
9331

CBI22983
270234210
0.8119

Vitis vinifera

7749

XP_002328602
255761085
0.7953

Populus trichocarpa

7750

BAG16526
171854672
0.7898

Capsicum chinense

7751
9332

Q39659

0.7621

Cucumis sativus

7752

NP_187342
145338207
0.7759

Arabidopsis thaliana

7753
9333

XP_002882497
297853636
0.7718

Arabidopsis lyrata

7754

subsp. lyrata

O49809

0.7552

Brassica napus

7755

176-195
NP_001235442
351722139
1

Glycine max

7756
9334

NP_001236219
351722401
0.9712

Glycine max

7757
9335

ABC46708
83776785
0.8417

Arachis hypogaea

7758
9336

Q40519

0.8201

Nicotiana tabacum

7759

NP_001234042
350537546
0.8273

Solanum lycopersicum

7760
9337

P06183

0.8201

Solanum tuberosum

7761

ADB93062
284520973
0.8273

Jatropha curcas

7762
9338

CAA27989
21490
0.8058

Solanum tuberosum

7763
9339

XP_002332206
255761085
0.7914

Populus trichocarpa

7764

ABK96223
118488825
0.7914

Populus trichocarpa ×
7765
9340

Populus deltoides

80-99
ACJ85304
217073887
1

Medicago truncatula

7766
9341

ABF66654
120650107
0.9086

Ammopiptanthus mongolicus

7767
9342

ACU19677
255638744
0.9213

Glycine max

7768
9343

CBI18248
270229319
0.8807

Vitis vinifera

7769

XP_002530504
255761086
0.8858

Ricinus communis

7770

XP_002308228
255761085
0.8756

Populus trichocarpa

7771

XP_002262986
225439379
0.8807

Vitis vinifera

7772
9344

ACU23134
255645273
0.8858

Glycine max

7773
9345

ABJ97690
116292767
0.8503

Solanum tuberosum

7774
9346

XP_002322994
255761085
0.7995

Populus trichocarpa

7775

488-507
ACU23010
255645020
1

Glycine max

7776
9347

989-1008
XP_002314377
255761085
1

Populus trichocarpa

7777

NP_001234975
351723428
0.814

Glycine max

7778
9348

82-101
ABF66654
120650107
1

Ammopiptanthus mongolicus

7779
9349

ACJ85304
217073887
0.9266

Medicago truncatula

7780
9350

91-110
ACU22749
255644490
1

Glycine max

7781
9351

ath-
256-277
NP_001238139
351726107
1

Glycine max

7782
9352

miRf10451-

NP_001237827
351724486
0.9394

Glycine max

7783
9353

akr

NP_001237200
351721157
0.8838

Glycine max

7784
9354

NP_001236083
351725850
0.8333

Glycine max

7785
9355

143-164
NP_001238139
351726107
1

Glycine max

7786
9356

ath-
188-207
ACU18963
255637265
1

Glycine max

7787
9357

miRf10751-

ACU21242
255641949
0.7764

Glycine max

7788
9358

akr
23-42
ACU20965
255641375
1

Glycine max

7789
9359

XP_002301406
255761085
0.8099

Populus trichocarpa

7790

XP_002320196
255761085
0.7851

Populus trichocarpa

7791

XP_002281449
225454509
0.7879

Vitis vinifera

7792
9360

NP_566074
145361064
0.7245

Arabidopsis thaliana

7793
9361

XP_002880239
297853636
0.7245

Arabidopsis lyrata

7794

subsp. lyrata

AAU93592
53793715
0.7135

Solanum demissum

7795
9362

XP_002876635
297853636
0.719

Arabidopsis lyrata

7796

subsp. lyrata

NP_191729
145339747
0.7107

Arabidopsis thaliana

7797
9363

1857-1876
XP_002525341
255761086
1

Ricinus communis

7798

XP_002326656
255761085
0.8901

Populus trichocarpa

7799

CAN79431
147866563
0.8524

Vitis vinifera

7800

NP_001235564
351725644
0.8599

Glycine max

7801
9364

ADW84019
321438026
0.8419

Gossypium hirsutum

7802
9365

XP_002303363
255761085
0.8584

Populus trichocarpa

7803

2FON_A

0.8238

Solanum lycopersicum

7804

NP_001234198
350535510
0.8238

Solanum lycopersicum

7805
9366

AAW78691
58531951
0.8223

Solanum cheesmaniae

7806

XP_002868103
297853636
0.8313

Arabidopsis lyrata

7807

subsp. lyrata

738-757
CAD31838
21068663
1

Cicer arietinum

7808
9367

NP_001237954
351720733
0.9412

Glycine max

7809
9368

ACU19740
255638874
0.9314

Glycine max

7810
9369

NP_001237941
351727802
0.9118

Glycine max

7811
9370

XP_002518592
255761086
0.8824

Ricinus communis

7812

XP_002534445
255761086
0.8873

Ricinus communis

7813

AAD38143
5031274
0.8676

Prunus armeniaca

7814
9371

XP_002283286
225461208
0.8627

Vitis vinifera

7815
9372

ABN12320
124488471
0.848

Gossypium hirsutum

7816
9373

XP_002867516
297853636
0.8578

Arabidopsis lyrata

7817

subsp. lyrata

188-207
ACU18963
255637265
1

Glycine max

7818
9374

87-106
ACU21242
255641949
1

Glycine max

7819
9375

XP_002285386
225430399
0.7263

Vitis vinifera

7820
9376

CBI21096
270231236
0.7263

Vitis vinifera

7821

387-406
ABP88240
145652370
1

Glycine max

7822
9377

ppt-
182-202
ACU23202
255645411
1

Glycine max

7823
9378

miR1220a

BAG06274
318612463
0.8691

Vigna unguiculata

7824
9379

CBI31552
270244444
0.7404

Vitis vinifera

7825

XP_002280217
225449239
0.7404

Vitis vinifera

7826
9380

XP_002316242
255761085
0.7652

Populus trichocarpa

7827

NP_181518
30688068
0.7111

Arabidopsis thaliana

7828
9381

XP_002879830
297853636
0.7133

Arabidopsis lyrata

7829

subsp. lyrata

AAM65420
21406633
0.7088

Arabidopsis thaliana

7830
9382

NP_191133
42565959
0.7314

Arabidopsis thaliana

7831
9383

XP_002876330
297853636
0.7246

Arabidopsis lyrata

7832

subsp. lyrata

539-559
NP_001234951
351722740
1

Glycine max

7833
9384

182-202
ACU23202
255645411
1

Glycine max

7834
9385

233-253
ACU22926
255644851
1

Glycine max

7835
9386

ACU23107
255645218
0.8212

Glycine max

7836
9387

ABU93486
156739649
0.7848

Vigna angularis

7837
9388

ABK30788
116871383
0.745

Litchi chinensis

7838
9389

AAK51119
14029148
0.7152

Carica papaya

7839
9390

XP_002523709
255761086
0.7053

Ricinus communis

7840

CAA48324
311834
0.7119

Tropaeolum majus

7841
9391

XP_002275862
225436483
0.7219

Vitis vinifera

7842
9392

125-145
BAG06274
318612463
1

Vigna unguiculata

7843
9393

ath-
17-36
ABY78023
166014266
1

Glycine max

7844
9394

miRf10068-

XP_002310310
255761085
0.815

Populus trichocarpa

7845

akr

XP_002269295
225467972
0.7974

Vitis vinifera

7846
9395

AAX47170
61611670
0.7665

Pisum sativum

7847
9396

ACY82403
267850662
0.793

Petunia × hybrida
7848
9397

CAG27846
83999599
0.7665

Antirrhinum majus

7849
9398

CAL36572
113207064
0.7621

Misopates orontium

7850
9399

ABD66219
122056646
0.7665

Malus × domestica
7851
9400

AAF22455
6652755
0.7709

Paulownia kawakamii

7852
9401

AAP40641
30983947
0.7533

Eucalyptus occidentalis

7853
9402

816-835
AEH04452
334813894
1

Arachis hypogaea

7854
9403

XP_002512790
255761086
0.9358

Ricinus communis

7855

ACU21011
255641470
0.9309

Glycine max

7856
9404

NP_001238484
351721287
0.9309

Glycine max

7857
9405

P12858

0.9185

Pisum sativum

7858

CAA33264
20728
0.916

Pisum sativum

7859
9406

ACV32597
256862073
0.9185

Medicago sativa

7860
9407

BAJ34149
312282566
0.8938

Thellungiella halophila

7861
9408

ACT21568
251831337
0.8963

Bruguiera gymnorhiza

7862
9409

ADX97321
323650480
0.9185

Mangifera indica

7863
9410

898-917
AEH04452
334813894
1

Arachis hypogaea

7864
9411

121-140
ABC68403
85001696
1

Glycine max

7865
9412

XP_002275806
225426452
0.7992

Vitis vinifera

7866
9413

CAN80040
147844259
0.7992

Vitis vinifera

7867
9414

XP_002509820
255761086
0.803

Ricinus communis

7868

XP_002304502
255761085
0.7765

Populus trichocarpa

7869

AAZ39642
71726941
0.7708

Petunia × hybrida
7870
9415

XP_002320802
255761085
0.7557

Populus trichocarpa

7871

XP_002275115
225454267
0.7614

Vitis vinifera

7872
9416

CAN80156
147852118
0.7557

Vitis vinifera

7873
9417

XP_002882043
297853636
0.75

Arabidopsis lyrata

7874

subsp. lyrata

621-640
NP_001238484
351721287
1

Glycine max

7875
9418

AEH04452
334813894
0.9355

Arachis hypogaea

7876
9419

ABK96233
118488846
0.8958

Populus trichocarpa ×
7877
9420

Populus deltoides

419-438
ACU19391
255638154
1

Glycine max

7878
9421

511-530
CAC80373
18072796
1

Capsicum annuum

7879
9422

CAC80372
18072794
0.9583

Capsicum annuum

7880
9423

P09043

0.9263

Nicotiana tabacum

7881

osa-
56-78
ACU19975
255639357
1

Glycine max

7882
9424

miRf11352-

ACU19227
255637811
0.9593

Glycine max

7883
9425

akr

ACU19215
255637786
0.7647

Glycine max

7884
9426

XP_002513621
255761086
0.7059

Ricinus communis

7885

XP_002318354
255761085
0.724

Populus trichocarpa

7886

ath-
134-154
NP_001236767
351723442
1

Glycine max

7887
9427

miRf11021-
18-38
ACJ84983
217073247
1

Medicago truncatula

7888
9428

akr

O48905

0.991

Medicago sativa

7889

CAC10208
10334492
0.9639

Cicer arietinum

7890
9429

NP_001236661
351727792
0.9518

Glycine max

7891
9430

XP_002332745
255761085
0.9428

Populus trichocarpa

7892

XP_002533463
255761086
0.9398

Ricinus communis

7893

AEB60994
328908588
0.9488

Lupinus angustifolius

7894
9431

XP_002312583
255761085
0.9367

Populus trichocarpa

7895

CAH58641
52851185
0.9247

Plantago major

7896
9432

ABC01890
83283964
0.9337

Solanum tuberosum

7897
9433

aly-
234-256
XP_002513787
255761086
1

Ricinus communis

7898

miR831-5p

XP_002337051
255761085
0.7

Populus trichocarpa

7899

XP_002300997
255761085
0.7174

Populus trichocarpa

7900

far-
222-245
ACF22880
193850552
1

Glycine max

7901
9434

miR1134

XP_002325840
255761085
0.7248

Populus trichocarpa

7902

XP_002281809
225441606
0.7202

Vitis vinifera

7903
9435

ABK95741
118487835
0.7294

Populus trichocarpa

7904
9436

XP_002319160
255761085
0.7248

Populus trichocarpa

7905

XP_002525421
255761086
0.7018

Ricinus communis

7906

48-71
CAD31838
21068663
1

Cicer arietinum

7907
9437

NP_001237954
351720733
0.9412

Glycine max

7908
9438

ACU19740
255638874
0.9314

Glycine max

7909
9439

NP_001237941
351727802
0.9118

Glycine max

7910
9440

XP_002518592
255761086
0.8824

Ricinus communis

7911

XP_002534445
255761086
0.8873

Ricinus communis

7912

AAD38143
5031274
0.8676

Prunus armeniaca

7913
9441

XP_002283286
225461208
0.8627

Vitis vinifera

7914
9442

ABN12320
124488471
0.848

Gossypium hirsutum

7915
9443

XP_002867516
297853636
0.8578

Arabidopsis lyrata

7916

subsp. lyrata

39-62
AAD49742
5733805
1

Pisum sativum

7917
9444

AAM97354
22476945
0.9894

Pisum sativum

7918
9445

AAD33959
4929351
0.9814

Pisum sativum

7919
9446

ACU21225
255641912
0.9204

Glycine max

7920
9447

XP_002313052
255761085
0.9072

Populus trichocarpa

7921

XP_002284375
225428500
0.8992

Vitis vinifera

7922
9448

XP_002509478
255761086
0.8992

Ricinus communis

7923

XP_002306098
255761085
0.8992

Populus trichocarpa

7924

P93563

0.8674

Solanum tuberosum

7925

P93397

0.87

Nicotiana tabacum

7926

180-203
ACC85689
186477889
1

Medicago truncatula

7927
9449

NP_001235733
351734425
0.8957

Glycine max

7928
9450

XP_002318640
255761085
0.872

Populus trichocarpa

7929

XP_002322155
255761085
0.8768

Populus trichocarpa

7930

AEQ62558
352740725
0.8815

Aquilaria microcarpa

7931
9451

NP_001048088
115448616
0.8483

Oryza sativa

7932
9452

Japonica Group

NP_201093
186532680
0.8531

Arabidopsis thaliana

7933
9453

NP_566897
30692961
0.8483

Arabidopsis thaliana

7934
9454

CAD42725
27527522
0.8436

Nicotiana tabacum

7935
9455

XP_002511439
255761086
0.8578

Ricinus communis

7936

819-842
NP_001235100
351727055
1

Glycine max

7937
9456

CBZ41765
323669526
0.8864

Glycine max

7938
9457

CCD42020
347630190
0.8701

Glycine max

7939
9458

180-203
ACC85689
186477889
1

Medicago truncatula

7940
9459

429-452
NP_001238108
351725208
1

Glycine max

7941
9460

NP_001238275
351722648
0.9264

Glycine max

7942
9461

ath-
1224-1244
ACO48252
226320261
1

Arachis hypogaea

7943
9462

miRf10687-

NP_001237378
351726308
0.889

Glycine max

7944
9463

akr

ABR29877
149789411
0.8301

Ricinus communis

7945
9464

XP_002280842
225423836
0.8205

Vitis vinifera

7946
9465

CAN62388
147809569
0.8127

Vitis vinifera

7947

XP_002893416
297853636
0.7954

Arabidopsis lyrata

7948

subsp. lyrata

XP_002888679
297853636
0.7597

Arabidopsis lyrata

7949

subsp. lyrata

NP_177043
42563058
0.7558

Arabidopsis thaliana

7950
9466

ACT54615
254032061
0.779

Brassica napus

7951
9467

ACN39927
224284384
0.7413

Picea sitchensis

7952
9468

735-755
ACU24612
255648320
1

Glycine max

7953
9469

ACU19270
255637904
0.771

Glycine max

7954
9470

XP_002311695
255761085
0.7252

Populus trichocarpa

7955

585-605
ACU18495
255636311
1

Glycine max

7956
9471

1116-1136
ACJ85683
217074645
1

Medicago truncatula

7957
9472

NP_001235116
351727520
0.8244

Glycine max

7958
9473

BAD81043
56744206
0.8189

Glycine max

7959
9474

O82709

0.8743

Pisum sativum

7960

AAK84429
31321895
0.7301

Brassica napus

7961
9475

XP_002866454
297853636
0.7227

Arabidopsis lyrata

7962

subsp. lyrata

NP_200987
145359541
0.7246

Arabidopsis thaliana

7963
9476

XP_002511066
255761086
0.7301

Ricinus communis

7964

XP_002277666
225447724
0.7227

Vitis vinifera

7965
9477

CAN83091
147858622
0.7227

Vitis vinifera

7966
9478

363-383
NP_001238368
351734499
1

Glycine max

7967
9479

291-311
NP_001237352
351734505
1

Glycine max

7968
9480

AAC32262
3426303
0.7348

Pisum sativum

7969
9481

NP_001238058
351723760
0.7652

Glycine max

7970
9482

AAV28488
54042994
0.7099

Populus tremula ×
7971
9483

Populus alba

XP_002518420
255761086
0.7155

Ricinus communis

7972

AAV49801
55276119
0.7127

Populus trichocarpa ×
7973
9484

Populus deltoides

ABO33478
132424650
0.732

Medicago truncatula

7974
9485

ADC35600
285804238
0.7155

Prunus persica

7975
9486

AAG27464
11037019
0.7293

Medicago truncatula

7976
9487

XP_002271944
225444459
0.7182

Vitis vinifera

7977
9488

24-44
XP_002509851
255761086
1

Ricinus communis

7978

XP_002304516
255761085
0.863

Populus trichocarpa

7979

XP_002298015
255761085
0.8527

Populus trichocarpa

7980

XP_002278860
225426165
0.8424

Vitis vinifera

7981
9489

AAN65180
25052803
0.8346

Petroselinum crispum

7982
9490

XP_002302599
255761085
0.832

Populus trichocarpa

7983

XP_002277669
225454333
0.8269

Vitis vinifera

7984
9491

XP_002511904
255761086
0.8217

Ricinus communis

7985

ACU20804
255641048
0.8191

Glycine max

7986
9492

Q40353

0.801

Medicago sativa

7987

ath-
93-113
AEA92304
327505552
1

Hevea brasiliensis

7988
9493

miRf11037-

ADL59582
302595186
0.9815

Hevea brasiliensis

7989
9494

akr

XP_002284365
225435057
0.9213

Vitis vinifera

7990
9495

CAN64127
147783306
0.9213

Vitis vinifera

7991
9496

XP_002534292
255761086
0.9259

Ricinus communis

7992

BAB84326
18447920
0.9491

Nicotiana tabacum

7993
9497

AEA92307
327505558
0.9213

Hevea brasiliensis

7994
9498

BAB84324
18447916
0.912

Nicotiana tabacum

7995
9499

XP_002284071
225449602
0.9167

Vitis vinifera

7996
9500

ACU20932
255641309
0.9213

Glycine max

7997
9501

mtr-
498-518
ACJ84572
217072423
1

Medicago truncatula

7998
9502

miR2119

ACU20200
255639813
0.8895

Glycine max

7999
9503

XP_002520842
255761086
0.8226

Ricinus communis

8000

CBI25388
270236032
0.8072

Vitis vinifera

8001
9504

XP_002325811
255761085
0.8046

Populus trichocarpa

8002

Q42967

0.7841

Nicotiana tabacum

8003

1J93_A

0.7738

Nicotiana tabacum

8004

XP_002274385
225448634
0.7584

Vitis vinifera

8005
9505

XP_002879873
297853636
0.7661

Arabidopsis lyrata

8006

subsp. lyrata

NP_001050049
115452896
0.7506

Oryza sativa

8007
9506

Japonica Group

153-173
CAA80691
452768
1

Phaseolus acutifolius

8008
9507

CAA80692
452766
0.9974

Phaseolus acutifolius

8009
9508

AAO72531
29373060
0.9421

Lotus corniculatus

8010
9509

CAG30579
51587337
0.9395

Lotus japonicus

8011
9510

P13603

0.9237

Trifolium repens

8012

P12886

0.9184

Pisum sativum

8013

XP_002309899
255761085
0.8868

Populus trichocarpa

8014

XP_002328464
255761085
0.8737

Populus trichocarpa

8015

ABK95643
118487635
0.8789

Populus trichocarpa

8016
9511

XP_002309900
255761085
0.8763

Populus trichocarpa

8017

osa-
514-534
ACU20018
255639446
1

Glycine max

8018
9512

miR2055

ptc-
129-151
XP_003527653
356517960
1

Glycine max

8019
9513

miRf10132-

XP_003523542
356509614
0.7828

Glycine max

8020
9514

akr

tae-
89-110
ABC47858
83853825
1

Glycine max

8021
9515

miR2003
1828-1849
ABC47841
83853806
1

Glycine max

8022
9516

XP_002283105
225438780
0.7143

Vitis vinifera

8023
9517

BAJ53195
317106690
0.7032

Jatropha curcas

8024

XP_002284923
225458677
0.746

Vitis vinifera

8025
9518

BAJ53194
317106690
0.7095

Jatropha curcas

8026

XP_002301171
255761085
0.7524

Populus trichocarpa

8027

XP_002327166
255761085
0.746

Populus trichocarpa

8028

CBI19489
270252251
0.7397

Vitis vinifera

8029

XP_002510185
255761086
0.7111

Ricinus communis

8030

AAK30205
13560782
0.7016

Daucus carota

8031
9519

osa-
166-186
AAA74456
500752
1

Phaseolus vulgaris

8032
9520

miRf11829-

XP_003529397
356521507
0.933

Glycine max

8033
9521

akr

XP_003518837
356500028
0.9175

Glycine max

8034
9522

XP_003607969
357475366
0.8763

Medicago truncatula

8035
9523

AAB50233
1906001
0.9021

Glycine max

8036
9524

CAC06095
9968472
0.8797

Lotus japonicus

8037
9525

ADJ68001
300119951
0.8488

Gossypium hirsutum

8038
9526

XP_002518763
255761086
0.8265

Ricinus communis

8039

XP_002330328
255761085
0.8196

Populus trichocarpa

8040

ABK95605
118487556
0.8179

Populus trichocarpa

8041
9527

134-154
CBI32147
270260094
1

Vitis vinifera

8042
9528

XP_002315592
255761085
0.8512

Populus trichocarpa

8043

XP_003556331
356576422
0.8095

Glycine max

8044
9529

XP_002262666
225424668
0.9683

Vitis vinifera

8045
9530

XP_002312609
255761085
0.8333

Populus trichocarpa

8046

XP_003536266
356535465
0.8036

Glycine max

8047
9531

XP_003590703
357440850
0.8472

Medicago truncatula

8048
9532

ADN33838
307135962
0.871

Cucumis melo

8049
9533

subsp. melo

ACF86937
194705705
0.756

Zea mays

8050
9534

NP_001152185
226494944
0.7421

Zea mays

8051
9535

345-365
XP_003522862
356508229
1

Glycine max

8052
9536

XP_003533465
356529778
0.9262

Glycine max

8053
9537

302-322
XP_003546711
356556804
1

Glycine max

8054
9538

XP_003542817
356548860
0.9169

Glycine max

8055
9539

XP_003627885
357515192
0.7569

Medicago truncatula

8056
9540

236-256
XP_003526444
356515512
1

Glycine max

8057

CBI20954
270231236
0.7572

Vitis vinifera

8058

XP_003603503
357466436
0.758

Medicago truncatula

8059

XP_002516594
255761086
0.7337

Ricinus communis

8060

XP_002308627
255761085
0.7281

Populus trichocarpa

8061

XP_002282016
225430126
0.756

Vitis vinifera

8062

564-584
XP_002509464
255761086
1

Ricinus communis

8063

XP_002329785
255761085
0.8641

Populus trichocarpa

8064

XP_003543041
356549318
0.8345

Glycine max

8065
9541

XP_002305792
255761085
0.8746

Populus trichocarpa

8066

XP_003545990
356555337
0.8537

Glycine max

8067
9542

NP_193830
30685267
0.8084

Arabidopsis thaliana

8068
9543

AAL38704
17528987
0.8066

Arabidopsis thaliana

8069
9544

XP_002869916
297853636
0.8049

Arabidopsis lyrata

8070

subsp. lyrata

ABF69959
102139737
0.7787

Musa acuminata

8071
9545

XP_002863589
297853636
0.7805

Arabidopsis lyrata

8072

subsp. lyrata

92-112
AAT35563
47558925
1

Phaseolus vulgaris

8073
9546

NP_001237920
351727189
0.8889

Glycine max

8074
9547

XP_003556188
356576132
0.8468

Glycine max

8075
9548

CAA06615
3413499
0.7387

Pisum sativum

8076
9549

30-50
XP_003530858
356524482
1

Glycine max

8077
9550

123-143
XP_003629209
357517840
1

Medicago truncatula

8078
9551

XP_003520082
356502550
0.8531

Glycine max

8079
9552

XP_003547906
356559235
0.8431

Glycine max

8080
9553

CBI20672
270231236
0.7496

Vitis vinifera

8081

XP_002279909
225429561
0.7613

Vitis vinifera

8082
9554

XP_002516094
255761086
0.7963

Ricinus communis

8083

BAK61816
343887266
0.7947

Citrus unshiu

8084
9555

XP_002308543
255761085
0.7813

Populus trichocarpa

8085

XP_002873542
297853636
0.7496

Arabidopsis lyrata

8086

subsp. lyrata

NP_568256
18416731
0.7379

Arabidopsis thaliana

8087
9556

427-447
ACI23460
207113464
1

Glycine soja

8088
9557

XP_003544485
356552257
0.9828

Glycine max

8089
9558

NP_001235613
351727089
0.8798

Glycine max

8090
9559

ACU20715
255640864
0.8197

Glycine max

8091
9560

212-232
NP_001236871
351726450
1

Glycine max

8092
9561

XP_003523441
356509406
0.9197

Glycine max

8093
9562

ACS94038
242877144
0.8294

Cicer arietinum

8094
9563

XP_003602038
357463512
0.8261

Medicago truncatula

8095
9564

ACD39411
187940570
0.7759

Arachis hypogaea

8096
9565

AER45736
354992034
0.786

Medicago sativa

8097
9566

XP_002520341
255761086
0.7425

Ricinus communis

8098

AEF80001
333696915
0.7258

Corylus heterophylla

8099
9567

ACI15342
206584338
0.7324

Gossypium hirsutum

8100
9568

ADL36795
302399000
0.7492

Malus × domestica
8101
9569

616-636
XP_003534059
356530984
1

Glycine max

8102
9570

XP_003548267
356559966
0.8665

Glycine max

8103
9571

XP_003619718
357498858
0.7211

Medicago truncatula

8104
9572

118-138
XP_003589961
357439368
1

Medicago truncatula

8105
9573

XP_003535239
356533372
0.8081

Glycine max

8106
9574

XP_003519713
356501802
0.8046

Glycine max

8107
9575

XP_002514955
255761086
0.7729

Ricinus communis

8108

XP_002315622
255761085
0.7817

Populus trichocarpa

8109

CAA58823
639833
0.7676

Solanum tuberosum

8110
9576

NP_001148767
226532264
0.7588

Zea mays

8111
9577

XP_003557368
357111130
0.7588

Brachypodium distachyon

8112
9578

BAJ94094
326490040
0.7711

Hordeum vulgare

8113
9579

subsp. vulgare

NP_196470
145357793
0.7835

Arabidopsis thaliana

8114
9580

92-112
XP_003597553
357454544
1

Medicago truncatula

8115
9581

XP_003542066
356547327
0.8553

Glycine max

8116
9582

XP_003546745
356556873
0.8496

Glycine max

8117
9583

XP_002514088
255761086
0.8177

Ricinus communis

8118

Q9MT28

0.7857

Solanum tuberosum

8119

XP_002285366
225430389
0.8233

Vitis vinifera

8120
9584

NP_194713
145349228
0.7989

Arabidopsis thaliana

8121
9585

AAB04607
1448916
0.7989

Arabidopsis thaliana

8122
9586

XP_002867385
297853636
0.7989

Arabidopsis lyrata

8123

subsp. lyrata

2C2B_A

0.7763

Arabidopsis thaliana

8124

135-155
XP_003541398
356545954
1

Glycine max

8125
9587

142-162
AAB50233
1906001
1

Glycine max

8126
9588

P38500

0.8037

Betula pendula

8127

123-143
XP_003547906
356559235
1

Glycine max

8128
9589

XP_003629209
357517840
0.8837

Medicago truncatula

8129
9590

TABLE 10

Target Genes of down-regulated Small RNA Molecules Associated with Abiotic Stress Tolerance in Soybean Plants.

Mir
Homolog
Nucleotide

Binding
NCBI
NCBI GI

Protein
Nucleotide

Mir Name
Position
Accession
number
Identity
Organism
Seq id no:
Seq id no:

aly-
116-136
XP_003531153
356525075
1

Glycine max

9591
10365

miR160c-

XP_003524859
356512301
0.936170213

Glycine max

9592
10366

3p

ABO61516
134142361
0.932301741

Glycine max

9593
10367

BAF62636
148189857
0.868471954

Phaseolus vulgaris

9594
10368

ABI34432
113206403
0.785299807

Pisum sativum

9595
10369

XP_002312450
255761085
0.767891683

Populus trichocarpa

9596

XP_002284648
225424290
0.735009671

Vitis vinifera

9597
10370

BAG16374
171702836
0.721470019

Brassica oleracea

9598
10371

var. italica

AEK06229
339779228
0.733075435

Vitis vinifera

9599
10372

bdi-
699-720
XP_003600994
357461424
1

Medicago truncatula

9600
10373

miR2508

XP_003538485
356540010
0.862608696

Glycine max

9601
10374

NP_001236616
351726477
0.850434783

Glycine max

9602
10375

XP_003519418
356501206
0.810434783

Glycine max

9603
10376

XP_003544045
356551362
0.812173913

Glycine max

9604
10377

XP_003616702
357492826
0.803478261

Medicago truncatula

9605
10378

XP_002520796
255761086
0.76

Ricinus communis

9606

XP_002315131
255761085
0.766956522

Populus trichocarpa

9607

ABK92474
118481040
0.768695652

Populus trichocarpa

9608
10379

XP_002312186
255761085
0.765217391

Populus trichocarpa

9609

719-740
XP_003520941
356504312
1

Glycine max

9610
10380

XP_003520942
356504314
0.820557491

Glycine max

9611
10381

XP_003516921
356496125
0.740418118

Glycine max

9612
10382

73-94
XP_003540719
356544563
1

Glycine max

9613
10383

XP_003539077
356541217
0.940068493

Glycine max

9614
10384

XP_003606701
357472832
0.821917808

Medicago truncatula

9615
10385

CBI16224
270227042
0.741438356

Vitis vinifera

9616

CAN60348
147789065
0.731164384

Vitis vinifera

9617
10386

XP_002313424
255761085
0.75

Populus trichocarpa

9618

XP_002284473
225435091
0.729452055

Vitis vinifera

9619
10387

XP_002278215
225449449
0.731164384

Vitis vinifera

9620
10388

CBI16199
270227042
0.724315068

Vitis vinifera

9621

651-672
XP_003516921
356496125
1

Glycine max

9622
10389

XP_003520941
356504312
0.75308642

Glycine max

9623
10390

77-98
XP_003522150
356506771
1

Glycine max

9624
10391

XP_003516941
356496165
0.967684022

Glycine max

9625
10392

XP_003604619
357468668
0.845601436

Medicago truncatula

9626
10393

XP_002322961
255761085
0.782764811

Populus trichocarpa

9627

XP_002533894
255761086
0.763016158

Ricinus communis

9628

XP_002278638
225440625
0.782764811

Vitis vinifera

9629
10394

XP_002322962
255761085
0.777378815

Populus trichocarpa

9630

XP_002308209
255761085
0.771992819

Populus trichocarpa

9631

XP_003552227
356568050
0.822262118

Glycine max

9632
10395

AAC49536
1685086
0.755834829

Nicotiana tabacum

9633
10396

86-107
CAN73336
147800866
1

Vitis vinifera

9634

XP_002282815
225449411
0.990859232

Vitis vinifera

9635
10397

XP_002282823
225449413
0.946983547

Vitis vinifera

9636
10398

XP_002278232
225449451
0.936014625

Vitis vinifera

9637
10399

CAN60069
147779995
0.92321755

Vitis vinifera

9638

CAN72263
147821463
0.92321755

Vitis vinifera

9639
10400

XP_002278275
225449453
0.91773309

Vitis vinifera

9640
10401

585-606
XP_003516921
356496125
1

Glycine max

9641
10402

549-570
XP_003606701
357472832
1

Medicago truncatula

9642
10403

XP_003540719
356544563
0.839316239

Glycine max

9643
10404

XP_003623041
357505504
0.733333333

Medicago truncatula

9644
10405

XP_003551448
356566457
0.714529915

Glycine max

9645
10406

XP_003532315
356527432
0.712820513

Glycine max

9646
10407

164-185
XP_003529133
356520972
1

Glycine max

9647
10408

XP_003552215
356568025
0.954385965

Glycine max

9648
10409

XP_003529131
356520968
0.722807018

Glycine max

9649
10410

ABC59623
84626065
0.721052632

Pisum sativum

9650
10411

3846-3867
XP_003551446
356566453
1

Glycine max

9651

699-720
XP_003600994
357461424
1

Medicago truncatula

9652
10412

261-282
XP_003520941
356504312
1

Glycine max

9653
10413

576-597
XP_003604619
357468668
1

Medicago truncatula

9654
10414

XP_003522150
356506771
0.845601436

Glycine max

9655
10415

XP_002308208
255761085
0.777378815

Populus trichocarpa

9656

0-21
XP_003516941
356496165
1

Glycine max

9657
10416

645-666
CBI16199
270227042
1

Vitis vinifera

9658

CAN80346
147858024
0.924028269

Vitis vinifera

9659
10417

XP_003552160
356567914
0.85335689

Glycine max

9660
10418

XP_002329138
255761085
0.846289753

Populus trichocarpa

9661

XP_002299296
255761085
0.85335689

Populus trichocarpa

9662

XP_002531824
255761086
0.848056537

Ricinus communis

9663

690-711
XP_003530212
356523164
1

Glycine max

9664
10419

XP_003551482
356566526
0.955094991

Glycine max

9665
10420

XP_002308164
255761085
0.778929188

Populus trichocarpa

9666

XP_002531565
255761086
0.772020725

Ricinus communis

9667

CAA74105
3805963
0.773747841

Populus trichocarpa

9668
10421

XP_002300066
255761085
0.730569948

Populus trichocarpa

9669

681-702
XP_003530213
356523166
1

Glycine max

9670
10422

XP_003552179
356567952
0.915447154

Glycine max

9671
10423

XP_003532290
356527381
0.765853659

Glycine max

9672
10424

XP_002309069
255761085
0.733333333

Populus trichocarpa

9673

XP_002531562
255761086
0.726829268

Ricinus communis

9674

XP_002271006
225440401
0.713821138

Vitis vinifera

9675
10425

XP_002268628
225440403
0.713821138

Vitis vinifera

9676
10426

XP_002269038
225440405
0.707317073

Vitis vinifera

9677
10427

CBI30529
270242856
0.704065041

Vitis vinifera

9678

651-672
XP_003551482
356566526
1

Glycine max

9679
10428

XP_003530212
356523164
0.960069444

Glycine max

9680
10429

666-687
XP_003539958
356543013
1

Glycine max

9681
10430

XP_003551299
356566152
0.941605839

Glycine max

9682
10431

XP_003518300
356498931
0.879562044

Glycine max

9683
10432

XP_003544873
356553053
0.881386861

Glycine max

9684
10433

XP_003615575
357490574
0.855839416

Medicago truncatula

9685
10434

XP_002520425
255761086
0.822992701

Ricinus communis

9686

XP_002314124
255761085
0.812043796

Populus trichocarpa

9687

XP_002299828
255761085
0.813868613

Populus trichocarpa

9688

XP_002280416
225434677
0.79379562

Vitis vinifera

9689
10435

86-107
CAN73336
147800866
1

Vitis vinifera

9690

121-142
XP_003615575
357490574
1

Medicago truncatula

9691
10436

XP_003539958
356543013
0.777403035

Glycine max

9692
10437

1113-1134
XP_003548937
356561332
1

Glycine max

9693
10438

XP_003519950
356502284
0.814741036

Glycine max

9694
10439

XP_002319173
255761085
0.802788845

Populus trichocarpa

9695

XP_002325825
255761085
0.794820717

Populus trichocarpa

9696

CAN70030
147821579
0.790836653

Vitis vinifera

9697
10440

XP_002525455
255761086
0.778884462

Ricinus communis

9698

XP_002304847
255761085
0.780876494

Populus trichocarpa

9699

XP_003625586
357510594
0.784860558

Medicago truncatula

9700
10441

XP_003607828
357475084
0.778884462

Medicago truncatula

9701
10442

XP_002523396
255761086
0.782868526

Ricinus communis

9702

633-654
XP_003551299
356566152
1

Glycine max

9703
10443

1051-1072
XP_003520176
356502743
1

Glycine max

9704
10444

XP_003528495
356519673
0.93444227

Glycine max

9705
10445

XP_003608057
357475542
0.777886497

Medicago truncatula

9706
10446

636-657
XP_003552213
356568021
1

Glycine max

9707
10447

XP_003529132
356520970
0.732517483

Glycine max

9708
10448

XP_003552214
356568023
0.723776224

Glycine max

9709
10449

XP_003529133
356520972
0.708041958

Glycine max

9710
10450

666-687
XP_003544873
356553053
1

Glycine max

9711
10451

666-687
XP_003552227
356568050
1

Glycine max

9712
10452

777-798
XP_003529132
356520970
1

Glycine max

9713
10453

XP_003552213
356568021
0.708551483

Glycine max

9714
10454

654-675
XP_003552215
356568025
1

Glycine max

9715
10455

660-681
XP_003529131
356520968
1

Glycine max

9716
10456

636-657
XP_003539077
356541217
1

Glycine max

9717
10457

XP_003551449
356566459
0.712328767

Glycine max

9718
10458

693-714
XP_003538485
356540010
1

Glycine max

9719
10459

CBI25418
270236032
0.785349233

Vitis vinifera

9720
10460

567-588
XP_003552179
356567952
1

Glycine max

9721
10461

XP_003530213
356523166
0.964041096

Glycine max

9722
10462

gma-
138-158
XP_003527195
356517033
1

Glycine max

9723
10463

miR2119

XP_003527162
356516966
0.885620915

Glycine max

9724
10464

XP_003527196
356517035
0.866013072

Glycine max

9725
10465

XP_003522929
356508367
0.81372549

Glycine max

9726
10466

XP_003522930
356508369
0.839869281

Glycine max

9727
10467

ACU24029
255647121
0.833333333

Glycine max

9728
10468

157-177
XP_003542005
356547201
1

Glycine max

9729
10469

ACU18712
255636755
0.994722955

Glycine max

9730
10470

XP_003545664
356554663
0.939313984

Glycine max

9731
10471

AAN03476
22597177
0.936675462

Glycine max

9732
10472

XP_003544738
356552774
0.802110818

Glycine max

9733
10473

AAO72531
29373060
0.807387863

Lotus corniculatus

9734
10474

CAA80691
452768
0.799472296

Phaseolus acutifolius

9735
10475

CAG30579
51587337
0.80474934

Lotus japonicus

9736
10476

AET21261
356582741
0.802110818

Lotus japonicus

9737
10477

P13603

0.799472296

Trifolium repens

9738

526-546
XP_003521584
356505611
1

Glycine max

9739
10478

XP_003554536
356572764
0.971098266

Glycine max

9740
10479

XP_003536003
356534928
0.809248555

Glycine max

9741
10480

XP_003518934
356500225
0.800578035

Glycine max

9742
10481

XP_002302739
255761085
0.789017341

Populus trichocarpa

9743

XP_002320324
255761085
0.777456647

Populus trichocarpa

9744

NP_191825
145339785
0.757225434

Arabidopsis thaliana

9745
10482

XP_002876684
297853636
0.757225434

Arabidopsis lyrata

9746

subsp. lyrata

XP_002872855
297853636
0.760115607

Arabidopsis lyrata

9747

subsp. lyrata

CAB83116
7362737
0.748554913

Arabidopsis thaliana

9748
10483

2061-2081
XP_003524240
356511040
1

Glycine max

9749
10484

XP_003532800
356528417
0.955097087

Glycine max

9750
10485

XP_003630005
357519432
0.82038835

Medicago truncatula

9751
10486

XP_002317684
255761085
0.770631068

Populus trichocarpa

9752

XP_002332198
255761085
0.766990291

Populus trichocarpa

9753

XP_003533825
356530512
0.769417476

Glycine max

9754
10487

XP_003547559
356558531
0.764563107

Glycine max

9755
10488

XP_002271023
225444212
0.751213592

Vitis vinifera

9756
10489

399-419
XP_003539263
356541601
1

Glycine max

9757
10490

XP_003517354
356497002
0.913716814

Glycine max

9758
10491

XP_003611556
357482538
0.767699115

Medicago truncatula

9759
10492

CAI79403
62700758
0.71460177

Senna occidentalis

9760
10493

265-285
XP_003554536
356572764
1

Glycine max

9761
10494

XP_003521584
356505611
0.971098266

Glycine max

9762
10495

XP_003625940
357511302
0.75433526

Medicago truncatula

9763
10496

159-179
XP_003545664
356554663
1

Glycine max

9764
10497

XP_003542005
356547201
0.959568733

Glycine max

9765
10498

P12886

0.789757412

Pisum sativum

9766

360-380
AAO83155
29365515
1

Phaseolus vulgaris

9767
10499

XP_003534097
356531061
0.870201097

Glycine max

9768
10500

XP_003548308
356560048
0.886654479

Glycine max

9769
10501

2055-2075
XP_003532800
356528417
1

Glycine max

9770
10502

XP_003524240
356511040
0.955097087

Glycine max

9771
10503

2157-2177
XP_003547559
356558531
1

Glycine max

9772
10504

174-194
XP_003517354
356497002
1

Glycine max

9773
10505

XP_003539263
356541601
0.919821826

Glycine max

9774
10506

gso-
22-42
AAU95080
53830374
1

Glycine max

9775
10507

miR482a
22-42
AAF44087
7263110
1

Glycine max

9776
10508

AAX81296
62361234
0.714285714

Arachis hypogaea

9777
10509

1195-1215
NP_001237600
351725318
1

Glycine max

9778
10510

XP_003556265
356576288
0.891737892

Glycine max

9779
10511

XP_003591822
357443088
0.811965812

Medicago truncatula

9780
10512

ACI46678
209419748
0.811965812

Galega orientalis

9781
10513

XP_003518682
356499714
0.740740741

Glycine max

9782
10514

XP_003516851
356495985
0.752136752

Glycine max

9783
10515

XP_003614455
357488334
0.732193732

Medicago truncatula

9784
10516

503-523
XP_003533606
356530067
1

Glycine max

9785
10517

59-79
XP_003518623
356499593
1

Glycine max

9786
10518

XP_003591325
357442094
0.832673267

Medicago truncatula

9787
10519

XP_003626036
357511494
0.751485149

Medicago truncatula

9788
10520

XP_002515202
255761086
0.7

Ricinus communis

9789

22-42
AAF44087
7263110
1

Glycine max

9790
10521

osa-
342-362
XP_003590416
357440276
1

Medicago truncatula

9791
10522

miR162a

XP_003554409
356572505
0.775075988

Glycine max

9792
10523

XP_003521428
356505296
0.767477204

Glycine max

9793
10524

XP_003625731
357510884
0.770516717

Medicago truncatula

9794
10525

XP_002264567
225441081
0.734042553

Vitis vinifera

9795
10526

XP_003541439
356546037
0.759878419

Glycine max

9796
10527

CAN74141
147838148
0.705167173

Vitis vinifera

9797

XP_002519415
255761086
0.703647416

Ricinus communis

9798

619-639
XP_003528812
356520321
1

Glycine max

9799
10528

XP_003528810
356520317
0.979865772

Glycine max

9800
10529

XP_003548576
356560594
0.976510067

Glycine max

9801
10530

XP_003543259
356549760
0.963087248

Glycine max

9802
10531

XP_003543258
356549758
0.959731544

Glycine max

9803
10532

ACU23594
255646218
0.966442953

Glycine max

9804
10533

ACJ85054
217073389
0.89261745

Medicago truncatula

9805
10534

XP_002329431
255761085
0.879194631

Populus trichocarpa

9806

XP_003604056
357467542
0.848993289

Medicago truncatula

9807
10535

XP_002524558
255761086
0.82885906

Ricinus communis

9808

585-605
XP_003543259
356549760
1

Glycine max

9809
10536

XP_003528812
356520321
0.963087248

Glycine max

9810
10537

XP_002330691
255761085
0.859060403

Populus trichocarpa

9811

1037-1057
XP_002311013
255761085
1

Populus trichocarpa

9812

XP_002315438
255761085
0.935779817

Populus trichocarpa

9813

XP_002521182
255761086
0.862385321

Ricinus communis

9814

XP_003536707
356536360
0.834862385

Glycine max

9815
10538

XP_003555872
356575487
0.830275229

Glycine max

9816
10539

XP_002268975
225450253
0.811926606

Vitis vinifera

9817
10540

144-164
XP_003548576
356560594
1

Glycine max

9818
10541

63-83
XP_003528812
356520321
1

Glycine max

9819
10542

217-237
NP_001237819
351724250
1

Glycine max

9820
10543

NP_001238239
351721616
0.964285714

Glycine max

9821
10544

ACF06595
192912973
0.898809524

Elaeis guineensis

9822
10545

Q5J907

0.892857143

Elaeis guineensis

9823

ACF06596
192912975
0.904761905

Elaeis guineensis

9824
10546

ACF06557
192910897
0.875

Elaeis guineensis

9825
10547

XP_003577266
357155873
0.845238095

Brachypodium distachyon

9826
10548

XP_002516930
255761086
0.857142857

Ricinus communis

9827

AEH05972
334854631
0.863095238

Hevea brasiliensis

9828
10549

NP_001235906
351720717
0.851190476

Glycine max

9829
10550

osa-
615-634
XP_003531377
356525528
1

Glycine max

9830
10551

miR1846e

XP_003525073
356512737
0.958823529

Glycine max

9831
10552

XP_002310600
255761085
0.785294118

Populus trichocarpa

9832

XP_002307126
255761085
0.8

Populus trichocarpa

9833

XP_002280295
225438578
0.808823529

Vitis vinifera

9834
10553

XP_003523264
356509047
0.826470588

Glycine max

9835
10554

AAZ66923
37694873
0.708823529

Brassica rapa

9836
10555

ACK44524
217426787
0.708823529

Arabidopsis arenosa

9837
10556

XP_002871399
297853636
0.7

Arabidopsis lyrata

9838

subsp. lyrata

NP_196563
145357839
0.702941176

Arabidopsis thaliana

9839
10557

379-398
XP_003531668
356526120
1

Glycine max

9840
10558

XP_003529761
356522251
0.915980231

Glycine max

9841
10559

XP_003530142
356523023
0.756177924

Glycine max

9842
10560

XP_003546477
356556325
0.73476112

Glycine max

9843
10561

XP_003531667
356526118
0.731466227

Glycine max

9844
10562

114-133
XP_003529761
356522251
1

Glycine max

9845
10563

XP_003531668
356526120
0.917491749

Glycine max

9846
10564

XP_003597728
357454894
0.724422442

Medicago truncatula

9847
10565

367-386
XP_003530142
356523023
1

Glycine max

9848
10566

XP_003597726
357454890
0.700490998

Medicago truncatula

9849
10567

osa-
256-277
NP_001105847
162464254
1

Zea mays

9850

miR2104

ABA42672
76443928
0.881188119

Zea mays

9851

Q2N2K2

0.927392739

Glycine max

9852

XP_002447301
255761094
0.907590759

Sorghum bicolor

9853

ACG23902
195604143
0.811881188

Zea mays

9854
10568

256-277
NP_001105847
162464254
1

Zea mays

9855
10569

osa-
103-122
ABU94631
156754274
1

Phaseolus vulgaris

9856
10570

miRf11415-

XP_003536353
356535640
0.944250871

Glycine max

9857
10571

akr

XP_003556232
356576220
0.951219512

Glycine max

9858
10572

ACU24483
255648054
0.947735192

Glycine max

9859
10573

AAC17529
3158475
0.919860627

Samanea saman

9860
10574

ACJ85173
217073625
0.905923345

Medicago truncatula

9861
10575

XP_003548070
356559566
0.891986063

Glycine max

9862
10576

BAB40143
13486941
0.888501742

Pyrus communis

9863
10577

ACU20229
255639872
0.891986063

Glycine max

9864
10578

BAD90699
60498688
0.898954704

Mimosa pudica

9865
10579

ctr-miR171
477-497
XP_003538071
356539165
1

Glycine max

9866
10580

XP_003517966
356498249
0.849056604

Glycine max

9867
10581

pta-
17-37
XP_003627005
357513432
1

Medicago truncatula

9868
10582

miR166c

XP_003531652
356526088
0.921875

Glycine max

9869
10583

XP_003530109
356522957
0.923076923

Glycine max

9870
10584

XP_003530112
356522963
0.913461538

Glycine max

9871
10585

ACI13685
206572104
0.889423077

Malus × domestica
9872
10586

XP_003597690
357454818
0.894230769

Medicago truncatula

9873
10587

XP_002515977
255761086
0.887019231

Ricinus communis

9874

XP_002284003
225442500
0.890625

Vitis vinifera

9875
10588

CBI36079
270253379
0.890625

Vitis vinifera

9876

XP_003531653
356526090
0.900240385

Glycine max

9877
10589

87-107
CAN73584
147820217
1

Vitis vinifera

9878

XP_002281868
225444032
1

Vitis vinifera

9879
10590

XP_002298892
255761085
0.918343195

Populus trichocarpa

9880

XP_002332526
255761085
0.90887574

Populus trichocarpa

9881

XP_003535078
356533042
0.880473373

Glycine max

9882
10591

XP_003546255
356555874
0.882840237

Glycine max

9883
10592

AAS66760
45479745
0.878106509

Nicotiana sylvestris

9884
10593

XP_003532788
356528393
0.852071006

Glycine max

9885
10594

XP_003524993
356512573
0.840236686

Glycine max

9886
10595

ACI13683
206572100
0.820118343

Malus × domestica
9887
10596

40-60
AAS10176
41745611
1

Antirrhinum majus

9888
10597

1228-1248
XP_002285176
225435326
1

Vitis vinifera

9889
10598

CAN61612
147783603
0.981042654

Vitis vinifera

9890

XP_002529946
255761086
0.918246445

Ricinus communis

9891

XP_003538150
356539326
0.892180095

Glycine max

9892
10599

XP_003539764
356542618
0.895734597

Glycine max

9893
10600

ACI13684
206572102
0.888625592

Malus × domestica
9894
10601

XP_003539765
356542620
0.890995261

Glycine max

9895
10602

AAX19050
60327620
0.881516588

Populus trichocarpa

9896
10603

DAA05766
109729904
0.853080569

Lotus japonicus

9897

AAY33856
63115353
0.8507109

Gossypium barbadense

9898
10604

557-577
XP_002298892
255761085
1

Populus trichocarpa

9899

CAN73584
147820217
0.91943128

Vitis vinifera

9900

515-535
XP_003597690
357454818
1

Medicago truncatula

9901
10605

XP_002284014
225442502
0.897129187

Vitis vinifera

9902
10606

XP_002304217
255761085
0.888755981

Populus trichocarpa

9903

560-580
XP_002285176
225435326
1

Vitis vinifera

9904
10607

554-574
XP_003603630
357466690
1

Medicago truncatula

9905
10608

XP_003522716
356507930
0.943645084

Glycine max

9906
10609

XP_003526496
356515618
0.940047962

Glycine max

9907
10610

ACI13686
206572106
0.872901679

Malus × domestica
9908
10611

ADL36609
302398628
0.863309353

Malus × domestica
9909
10612

CBI20838
270231236
0.862110312

Vitis vinifera

9910

XP_002283717
225429913
0.862110312

Vitis vinifera

9911
10613

ACL51017
219879369
0.858513189

Citrus trifoliata

9912
10614

XP_002309538
255761085
0.868105516

Populus trichocarpa

9913

XP_002324794
255761085
0.857314149

Populus trichocarpa

9914

40-60
AAS10176
41745611
1

Antirrhinum majus

9915
10615

554-574
XP_003530109
356522957
1

Glycine max

9916
10616

590-610
XP_003524993
356512573
1

Glycine max

9917
10617

XP_003594520
357448488
0.781946073

Medicago truncatula

9918
10618

25-45
XP_003522716
356507930
1

Glycine max

9919
10619

XP_003603630
357466690
0.932464455

Medicago truncatula

9920
10620

560-580
XP_003530112
356522963
1

Glycine max

9921
10621

530-550
XP_003532788
356528393
1

Glycine max

9922
10622

87-107
CAN73584
147820217
1

Vitis vinifera

9923

566-586
XP_003531653
356526090
1

Glycine max

9924
10623

560-580
XP_003539764
356542618
1

Glycine max

9925
10624

XP_002285176
225435326
0.894674556

Vitis vinifera

9926
10625

CAC84906
18076735
0.829585799

Zinnia violacea

9927
10626

828-848
XP_003539765
356542620
1

Glycine max

9928
10627

ptc-
81-100
XP_003548151
356559731
1

Glycine max

9929
10628

miRf10976-

XP_003529873
356522477
0.888

Glycine max

9930
10629

akr

XP_003548400
356560236
0.849333333

Glycine max

9931
10630

249-268
NP_001238468
351720798
1

Glycine max

9932
10631

242-261
XP_003520705
356503828
1

Glycine max

9933
10632

XP_003553607
356570870
0.874626866

Glycine max

9934
10633

295-314
XP_003533044
356528917
1

Glycine max

9935
10634

XP_003529756
356522241
0.905759162

Glycine max

9936
10635

XP_003543627
356550505
0.732984293

Glycine max

9937
10636

XP_003546548
356556469
0.722513089

Glycine max

9938
10637

XP_003597684
357454806
0.712041885

Medicago truncatula

9939
10638

XP_003597685
357454808
0.701570681

Medicago truncatula

9940
10639

268-287
XP_003528486
356519654
1

Glycine max

9941
10640

XP_003520183
356502757
0.899071926

Glycine max

9942
10641

ptc-
66-85
XP_003524954
356512494
1

Glycine max

9943
10642

miRf11018-

XP_003531241
356525252
0.920604915

Glycine max

9944
10643

akr

AET04202
357518874
0.797731569

Medicago truncatula

9945
10644

AET04197
357518864
0.752362949

Medicago truncatula

9946
10645

AET04200
357518870
0.756143667

Medicago truncatula

9947
10646

ptc-
1236-1255
XP_002275990
225451234
1

Vitis vinifera

9948
10647

miRf11669-

XP_002512253
255761086
0.916923077

Ricinus communis

9949

akr

XP_003554689
356573071
0.895384615

Glycine max

9950
10648

XP_002319618
255761085
0.916923077

Populus trichocarpa

9951

XP_002336146
255761085
0.895384615

Populus trichocarpa

9952

XP_002328363
255761085
0.895384615

Populus trichocarpa

9953

ACU22789
255644572
0.886153846

Glycine max

9954
10649

ADN33908
307136046
0.898461538

Cucumis melo

9955
10650

subsp. melo

Q43317

0.898461538

Citrullus lanatus

9956

subsp. vulgaris

XP_002311629
255761085
0.901538462

Populus trichocarpa

9957

259-278
XP_003624868
357509158
1

Medicago truncatula

9958
10651

ACJ85972
217075223
0.995098039

Medicago truncatula

9959
10652

XP_003608106
357475640
0.965686275

Medicago truncatula

9960
10653

NP_001237278
351723416
0.965686275

Glycine max

9961
10654

NP_564149
30687501
0.931372549

Arabidopsis thaliana

9962
10655

NP_001237990
351721817
0.941176471

Glycine max

9963
10656

XP_002890456
297853636
0.926470588

Arabidopsis lyrata

9964

subsp. lyrata

NP_565156
186496015
0.93627451

Arabidopsis thaliana

9965
10657

XP_002887683
297853636
0.931372549

Arabidopsis lyrata

9966

subsp. lyrata

AAM62756
21404242
0.926470588

Arabidopsis thaliana

9967
10658

170-189
XP_003535921
356534761
1

Glycine max

9968
10659

XP_003519071
356500502
0.968379447

Glycine max

9969
10660

XP_002284060
225441833
0.773386034

Vitis vinifera

9970
10661

CAN82225
147852313
0.770750988

Vitis vinifera

9971
10662

XP_002532077
255761086
0.744400527

Ricinus communis

9972

XP_002323318
255761085
0.737812912

Populus trichocarpa

9973

XP_002308029
255761085
0.749670619

Populus trichocarpa

9974

CBI29841
270242856
0.753623188

Vitis vinifera

9975
10663

NP_565960
18405800
0.723320158

Arabidopsis thaliana

9976
10664

XP_002878149
297853636
0.72859025

Arabidopsis lyrata

9977

subsp. lyrata

120-139
AAQ57205
34099832
1

Populus tremula ×
9978
10665

Populus alba

877-896
XP_003526542
356515711
1

Glycine max

9979
10666

XP_003523783
356510109
0.846153846

Glycine max

9980
10667

588-607
XP_002264051
225429233
1

Vitis vinifera

9981
10668

CAN64867
123673833
0.996763754

Vitis vinifera

9982

XP_002513130
255761086
0.877022654

Ricinus communis

9983

XP_003525216
356513027
0.83171521

Glycine max

9984
10669

XP_003530931
356524629
0.828478964

Glycine max

9985
10670

ACU18834
255637002
0.828478964

Glycine max

9986
10671

XP_002319995
255761085
0.834951456

Populus trichocarpa

9987

BAH79622
240846167
0.822006472

Glycine max

9988

XP_002310364
255761085
0.805825243

Populus trichocarpa

9989

XP_003631000
357521422
0.812297735

Medicago truncatula

9990
10672

917-936
XP_003548812
356561077
1

Glycine max

9991
10673

XP_003525850
356514311
0.700787402

Glycine max

9992
10674

1246-1265
XP_003555457
356574646
1

Glycine max

9993
10675

XP_003543286
356549814
0.955497382

Glycine max

9994
10676

XP_003617150
357493722
0.756544503

Medicago truncatula

9995
10677

XP_002272310
225435984
0.712041885

Vitis vinifera

9996
10678

CBI24343
270235077
0.712041885

Vitis vinifera

9997

1320-1339
XP_003519686
356501748
1

Glycine max

9998
10679

XP_003547896
356559215
0.95412844

Glycine max

9999
10680

XP_003547897
356559217
0.95412844

Glycine max

10000
10681

XP_003517138
356496566
0.812844037

Glycine max

10001
10682

XP_003612325
357484076
0.783486239

Medicago truncatula

10002
10683

XP_003629155
357517732
0.798165138

Medicago truncatula

10003
10684

XP_003537681
356538378
0.8

Glycine max

10004
10685

602-621
XP_003520116
356502619
1

Glycine max

10005
10686

XP_002280702
225438602
0.856050955

Vitis vinifera

10006
10687

EAZ28751
54398660
0.829299363

Oryza sativa

10007

Japonica Group

214-233
XP_003554103
356571887
1

Glycine max

10008
10688

XP_003521108
356504648
0.923076923

Glycine max

10009
10689

ACU18694
255636716
0.919230769

Glycine max

10010
10690

XP_003624904
357509230
0.807692308

Medicago truncatula

10011
10691

XP_002526199
255761086
0.734615385

Ricinus communis

10012

XP_002283307
225440154
0.761538462

Vitis vinifera

10013
10692

XP_002307956
255761085
0.726923077

Populus trichocarpa

10014

XP_002876377
297853636
0.703846154

Arabidopsis lyrata

10015

subsp. lyrata

329-348
XP_003547896
356559215
1

Glycine max

10016
10693

XP_003519686
356501748
0.961182994

Glycine max

10017
10694

XP_002521706
255761086
0.715341959

Ricinus communis

10018

XP_002306425
255761085
0.71349353

Populus trichocarpa

10019

vvi-
209-228
XP_003601765
357462966
1

Medicago truncatula

10020
10695

miR394b

XP_003538543
356540129
0.843010753

Glycine max

10021
10696

XP_003551172
356565895
0.868817204

Glycine max

10022
10697

ACU23751
255646552
0.864516129

Glycine max

10023
10698

ACL51019
219879373
0.767741935

Citrus trifoliata

10024
10699

ACI13687
206572108
0.761290323

Malus × domestica
10025
10700

XP_002514903
255761086
0.746236559

Ricinus communis

10026

XP_002271194
225425399
0.739784946

Vitis vinifera

10027
10701

XP_002297845
255761085
0.750537634

Populus trichocarpa

10028

XP_003531199
356525167
0.735483871

Glycine max

10029
10702

1188-1207
XP_003551172
356565895
1

Glycine max

10030
10703

256-275
XP_003593155
357445754
1

Medicago truncatula

10031
10704

XP_003547599
356558613
0.890145396

Glycine max

10032
10705

XP_003547600
356558615
0.890145396

Glycine max

10033
10706

XP_003593158
357445760
0.843295638

Medicago truncatula

10034
10707

NP_567920
186515898
0.81098546

Arabidopsis thaliana

10035
10708

AEG25668
333952413
0.814216478

Gossypium hirsutum

10036
10709

AAK68074
14573458
0.809369952

Arabidopsis thaliana

10037
10710

ADE22249
292385867
0.822294023

Ageratina adenophora

10038
10711

XP_002867182
297853636
0.809369952

Arabidopsis lyrata

10039

subsp. lyrata

AEA76434
327422166
0.81098546

Gossypium hirsutum

10040
10712

268-287
XP_003524786
356512154
1

Glycine max

10041
10713

XP_002331852
255761085
0.762032086

Populus trichocarpa

10042

XP_002316738
255761085
0.751336898

Populus trichocarpa

10043

XP_002519740
255761086
0.748663102

Ricinus communis

10044

XP_003532647
356528107
0.71657754

Glycine max

10045
10714

CAN63784
147790991
0.703208556

Vitis vinifera

10046
10715

XP_002273992
225463405
0.703208556

Vitis vinifera

10047
10716

435-454
XP_003556143
356576040
1

Glycine max

10048
10717

XP_003556144
356576042
0.964285714

Glycine max

10049
10718

XP_003536435
356535807
0.932539683

Glycine max

10050
10719

XP_003536434
356535805
0.924603175

Glycine max

10051
10720

ACU19073
255637492
0.920634921

Glycine max

10052
10721

XP_003556145
356576044
0.94047619

Glycine max

10053
10722

XP_003536436
356535809
0.900793651

Glycine max

10054
10723

XP_003556146
356576046
0.912698413

Glycine max

10055
10724

XP_003592142
357443728
0.793650794

Medicago truncatula

10056
10725

ACU23298
255645607
0.777777778

Glycine max

10057
10726

475-494
XP_003531199
356525167
1

Glycine max

10058
10727

XP_003524898
356512380
0.911699779

Glycine max

10059
10728

ACU17886
255635055
0.905077263

Glycine max

10060
10729

1104-1123
XP_003538543
356540129
1

Glycine max

10061
10730

123-142
XP_003553643
356570944
1

Glycine max

10062
10731

XP_003521540
356505523
0.763779528

Glycine max

10063
10732

29-48
NP_001058751
115470304
1

Oryza sativa

10064
10733

Japonica Group

EAZ02551
54362548
0.987012987

Oryza sativa

10065

Indica Group

ACG30543
195617425
0.896103896

Zea mays

10066
10734

BAJ93722
326533897
0.896103896

Hordeum vulgare

10067
10735

subsp. vulgare

XP_002459234
255761094
0.883116883

Sorghum bicolor

10068

XP_003557668
357111736
0.896103896

Brachypodium distachyon

10069
10736

ACG28009
195612357
0.883116883

Zea mays

10070
10737

ACG24589
195605517
0.844155844

Zea mays

10071
10738

XP_003555981
356575711
0.87012987

Glycine max

10072
10739

XP_002284176
225438945
0.87012987

Vitis vinifera

10073
10740

1029-1048
XP_003524898
356512380
1

Glycine max

10074
10741

1245-1264
XP_003601765
357462966
1

Medicago truncatula

10075
10742

zma-
68-87
XP_002519732
255761086
1

Ricinus communis

10076

miR167u

XP_002298511
255761085
0.8183391

Populus trichocarpa

10077

XP_002317300
255761085
0.801038062

Populus trichocarpa

10078

XP_002272126
225463413
0.788927336

Vitis vinifera

10079
10743

XP_003524790
356512162
0.780276817

Glycine max

10080
10744

NP_180988
145360605
0.761245675

Arabidopsis thaliana

10081
10745

XP_003532649
356528111
0.780276817

Glycine max

10082
10746

XP_002879497
297853636
0.757785467

Arabidopsis lyrata

10083

subsp. lyrata

NP_568662
30694937
0.742214533

Arabidopsis thaliana

10084
10747

AAL11600
15983463
0.740484429

Arabidopsis thaliana

10085
10748

503-522
XP_003556422
356576607
1

Glycine max

10086
10749

XP_003556421
356576605
1

Glycine max

10087
10750

XP_003536179
356535285
0.754266212

Glycine max

10088
10751

109-128
XP_003533248
356529329
1

Glycine max

10089
10752

XP_003547372
356558152
0.944690265

Glycine max

10090
10753

XP_003550546
356564612
0.778761062

Glycine max

10091
10754

XP_003528627
356519941
0.783185841

Glycine max

10092
10755

XP_003609706
357478840
0.765486726

Medicago truncatula

10093
10756

XP_002273305
225431819
0.727876106

Vitis vinifera

10094
10757

CBI22951
270234210
0.727876106

Vitis vinifera

10095

1585-1604
XP_003550723
356564979
1

Glycine max

10096
10758

XP_003550724
356564981
0.985380117

Glycine max

10097
10759

XP_003529499
356521718
0.956140351

Glycine max

10098
10760

XP_003529500
356521720
0.946393762

Glycine max

10099
10761

XP_003546300
356555968
0.819688109

Glycine max

10100
10762

XP_002314972
255761085
0.726120858

Populus trichocarpa

10101

CBI22841
270234210
0.730994152

Vitis vinifera

10102

XP_002519280
255761086
0.717348928

Ricinus communis

10103

335-354
XP_003516798
356495878
1

Glycine max

10104
10763

29-48
XP_003525532
356513666
1

Glycine max

10105
10764

24-43
XP_003529500
356521720
1

Glycine max

10106
10765

XP_003550723
356564979
0.959486166

Glycine max

10107
10766

XP_002883053
297853636
0.707509881

Arabidopsis lyrata

10108

subsp. lyrata

NP_001118648
186510162
0.70256917

Arabidopsis thaliana

10109
10767

671-690
XP_003533338
356529518
1

Glycine max

10110
10768

314-333
XP_003529470
356521656
1

Glycine max

10111
10769

XP_003556768
356577305
0.95687885

Glycine max

10112
10770

Q43088

0.80698152
Ribulose-bisphosphate
10113

carboxylase

1MLV_A

0.747433265

Pisum sativum

10114

2H21_A

0.743326489

Pisum sativum

10115

zma-
330-350
ABE91847
61675805
1

Medicago truncatula

10116
10771

miR396b-
330-350
ABE91847
61675805
1

Medicago truncatula

10117
10772

3p

aly-
330-350
ABE91847
61675805
1

Medicago truncatula

10118
10773

miR396a-
330-350
ABE91847
61675805
1

Medicago truncatula

10119
10774

3p

gma-
224-244
NP_001235045
351725442
1

Glycine max

10120
10775

miR4412-

XP_002274402
225425717
0.733905579

Vitis vinifera

10121
10776

3p

XP_002315800
255761085
0.703862661

Populus trichocarpa

10122

1796-1816
NP_001235618
351727227
1

Glycine max

10123
10777

gma-
177-198
AAX13306
60100357
1

Lotus japonicus

10124
10778

miR482b-

NP_001236130
351727233
0.959641256

Glycine max

10125
10779

5p

AAN15183
23194452
0.865470852

Gossypium hirsutum

10126
10780

AAY30856
63094568
0.874439462

Prunus dulcis

10127
10781

ABV60385
157674586
0.874439462

Carica papaya

10128

ABM69043
122938394
0.860986547

Gossypium hirsutum

10129
10782

ADD91578
291278193
0.878923767

Prunus serrulata

10130
10783

var. lannesiana

AAO20104
27763669
0.860986547

Momordica charantia

10131
10784

AAD01742
4103341
0.869955157

Cucumis sativus

10132
10785

ABQ85556
148535235
0.874439462

Prunus persica

10133
10786

339-360
BAG06679
166788446
1

Phaseolus vulgaris

10134
10787

ACU20774
255640988
0.995575221

Glycine max

10135
10788

ACU24523
255648136
0.977876106

Glycine max

10136
10789

AAM91028
57472398
0.977876106

Pisum sativum

10137
10790

XP_002272971
225462010
0.96460177

Vitis vinifera

10138
10791

ABW06389
157955930
0.938053097

Gossypium hirsutum

10139
10792

ABW06392
157955936
0.933628319

Gossypium hirsutum

10140
10793

ACJ86177
217075633
0.938053097

Medicago truncatula

10141
10794

XP_002532178
255761086
0.951327434

Ricinus communis

10142

ABW06390
157955932
0.938053097

Gossypium hirsutum

10143
10795

35-56
CAN81115
147863854
1

Vitis vinifera

10144
10796

ptc-
753-772
XP_002271271
225468315
1

Vitis vinifera

10145
10797

miRf11953-

XP_002532424
255761086
0.945652174

Ricinus communis

10146

akr

ACU20760
255640960
0.945652174

Glycine max

10147
10798

XP_002877709
297853636
0.940217391

Arabidopsis lyrata

10148

subsp. lyrata

NP_001235421
351721538
0.934782609

Glycine max

10149
10799

NP_001236131
351727263
0.940217391

Glycine max

10150
10800

NP_190556
145339306
0.934782609

Arabidopsis thaliana

10151
10801

ACU14878
255629066
0.940217391

Glycine max

10152
10802

XP_002866698
297853636
0.923913043

Arabidopsis lyrata

10153

subsp. lyrata

NP_569051
186532841
0.918478261

Arabidopsis thaliana

10154
10803

309-328
ACU18943
255637224
1

Glycine max

10155
10804

ACU23146
255645298
0.953405018

Glycine max

10156
10805

ACJ84492
217072263
0.810035842

Medicago truncatula

10157
10806

ABK94686
118485671
0.784946237

Populus trichocarpa

10158
10807

XP_002528545
255761086
0.76702509

Ricinus communis

10159

XP_002326541
255761085
0.741935484

Populus trichocarpa

10160

XP_002278543
225427135
0.752688172

Vitis vinifera

10161
10808

XP_002274164
225454758
0.734767025

Vitis vinifera

10162
10809

XP_002303431
255761085
0.749103943

Populus trichocarpa

10163

218-237
ACU18943
255637224
1

Glycine max

10164
10810

1421-1440
P08926

1

Pisum sativum

10165

BAE71311
84468455
0.957410562

Trifolium pratense

10166
10811

BAE71231
84468295
0.955706985

Trifolium pratense

10167
10812

BAE71302
84468437
0.954003407

Trifolium pratense

10168
10813

BAE71227
84468287
0.93867121

Trifolium pratense

10169
10814

ACJ85785
217074849
0.94548552

Medicago truncatula

10170
10815

AEO21430
346229112
0.906303237

Glycine max

10171
10816

XP_002313525
255761085
0.877342419

Populus trichocarpa

10172

AAC68501
3790440
0.889267462

Canavalia lineata

10173
10817

XP_002328161
255761085
0.865417376

Populus trichocarpa

10174

165-184
ACU23935
255646930
1

Glycine max

10175
10818

60-79
BAE71304
84468441
1

Trifolium pratense

10176
10819

P08926

0.947939262

Pisum sativum

10177

bna-
163-183
ACU23935
255646930
1

Glycine max

10178
10820

miR2111b-
1419-1439
P08926

1

Pisum sativum

10179

5p

BAE71311
84468455
0.957410562

Trifolium pratense

10180
10821

BAE71231
84468295
0.955706985

Trifolium pratense

10181
10822

BAE71302
84468437
0.954003407

Trifolium pratense

10182
10823

BAE71227
84468287
0.93867121

Trifolium pratense

10183
10824

ACJ85785
217074849
0.94548552

Medicago truncatula

10184
10825

AEO21430
346229112
0.906303237

Glycine max

10185
10826

XP_002313525
255761085
0.877342419

Populus trichocarpa

10186

AAC68501
3790440
0.889267462

Canavalia lineata

10187
10827

XP_002328161
255761085
0.865417376

Populus trichocarpa

10188

153-173
ACU23159
255645324
1

Glycine max

10189
10828

CAF04055
119391878
0.745098039

Nicotiana benthamiana

10190
10829

CAF25317
119391874
0.735294118

Capsicum annuum

10191
10830

XP_002306360
255761085
0.715686275

Populus trichocarpa

10192

XP_002279217
225438516
0.732026144

Vitis vinifera

10193
10831

XP_002530256
255761086
0.715686275

Ricinus communis

10194

CBI22773
270234152
0.722222222

Vitis vinifera

10195

CBI21530
270232045
0.732026144

Vitis vinifera

10196

163-183
ACU23935
255646930
1

Glycine max

10197
10832

58-78
BAE71304
84468441
1

Trifolium pratense

10198
10833

P08926

0.947939262

Pisum sativum

10199

ptc-
720-743
ACU24381
255647842
1

Glycine max

10200
10834

miRf11079-

NP_001238255
351722074
0.996688742

Glycine max

10201
10835

akr

NP_001235901
351728022
0.943708609

Glycine max

10202
10836

AAK84883
15148911
0.917218543

Phaseolus vulgaris

10203
10837

AEE99077
332739375
0.870860927

Medicago truncatula

10204
10838

XP_002529954
255761086
0.768211921

Ricinus communis

10205

XP_002310688
255761085
0.771523179

Populus trichocarpa

10206

XP_002307195
255761085
0.741721854

Populus trichocarpa

10207

69-92
AAK84883
15148911
1

Phaseolus vulgaris

10208
10839

603-626
AAK84883
15148911
1

Phaseolus vulgaris

10209
10840

720-743
NP_001235901
351728022
1

Glycine max

10210
10841

721-744
ACU24381
255647842
1

Glycine max

10211
10842

bra-
989-1009
XP_003530952
356524671
1

Glycine max

10212
10843

miR160a-

XP_003525194
356512983
0.970842333

Glycine max

10213
10844

3p

XP_003521511
356505464
0.904967603

Glycine max

10214
10845

XP_003592908
357445260
0.792656587

Medicago truncatula

10215
10846

ABE91931
61675804
0.792656587

Medicago truncatula

10216
10847

XP_003626539
357512500
0.795896328

Medicago truncatula

10217
10848

XP_002281426
225441572
0.791576674

Vitis vinifera

10218
10849

Q9XHM1

0.789416847

Medicago truncatula

10219

XP_003589347
357438142
0.782937365

Medicago truncatula

10220
10850

CAN81874
147860525
0.761339093

Vitis vinifera

10221
10851

gma-
328-349
XP_003521176
356504786
1

Glycine max

10222
10852

miR1507a

gma-
507-527
XP_003554498
356572687
1

Glycine max

10223
10853

miR1524

XP_003521507
356505456
0.941358025

Glycine max

10224
10854

XP_003535834
356534585
0.712962963

Glycine max

10225
10855

XP_003519022
356500404
0.712962963

Glycine max

10226
10856

ppt-
189-209
XP_003553029
356569688
1

Glycine max

10227
10857

miR166m

XP_003537529
356538072
0.931216931

Glycine max

10228
10858

XP_003601737
357462910
0.727513228

Medicago truncatula

10229
10859

557-577
XP_002298892
255761085
1

Populus trichocarpa

10230

CAN73584
147820217
0.91943128

Vitis vinifera

10231

XP_002332526
255761085
0.94549763

Populus trichocarpa

10232

XP_002281868
225444032
0.91943128

Vitis vinifera

10233
10860

XP_003535078
356533042
0.892180095

Glycine max

10234
10861

XP_003546255
356555874
0.893364929

Glycine max

10235
10862

AAS66760
45479745
0.86492891

Nicotiana sylvestris

10236
10863

XP_003532788
356528393
0.849526066

Glycine max

10237
10864

XP_003524993
356512573
0.845971564

Glycine max

10238
10865

ACI13683
206572100
0.816350711

Malus × domestica
10239
10866

515-535
XP_003597690
357454818
1

Medicago truncatula

10240
10867

XP_003531652
356526088
0.918660287

Glycine max

10241
10868

XP_003530109
356522957
0.921052632

Glycine max

10242
10869

XP_002284003
225442500
0.897129187

Vitis vinifera

10243
10870

CBI36079
270253379
0.897129187

Vitis vinifera

10244

XP_002515977
255761086
0.901913876

Ricinus communis

10245

XP_003530112
356522963
0.916267943

Glycine max

10246
10871

XP_002284014
225442502
0.897129187

Vitis vinifera

10247
10872

ACI13685
206572104
0.89354067

Malus × domestica
10248
10873

XP_002304217
255761085
0.888755981

Populus trichocarpa

10249

560-580
XP_002285176
225435326
1

Vitis vinifera

10250
10874

CAN61612
147783603
0.981042654

Vitis vinifera

10251

XP_002529946
255761086
0.918246445

Ricinus communis

10252

XP_003538150
356539326
0.892180095

Glycine max

10253
10875

XP_003539764
356542618
0.895734597

Glycine max

10254
10876

ACI13684
206572102
0.888625592

Malus × domestica
10255
10877

XP_003539765
356542620
0.890995261

Glycine max

10256
10878

AAX19050
60327620
0.881516588

Populus trichocarpa

10257
10879

DAA05766
109729904
0.853080569

Lotus japonicus

10258

AAY33856
63115353
0.8507109

Gossypium barbadense

10259
10880

554-574
XP_003603630
357466690
1

Medicago truncatula

10260
10881

XP_003522716
356507930
0.943645084

Glycine max

10261
10882

XP_003526496
356515618
0.940047962

Glycine max

10262
10883

ACI13686
206572106
0.872901679

Malus × domestica
10263
10884

ADL36609
302398628
0.863309353

Malus × domestica
10264
10885

CBI20838
270231236
0.862110312

Vitis vinifera

10265

XP_002283717
225429913
0.862110312

Vitis vinifera

10266
10886

ACL51017
219879369
0.858513189

Citrus trifoliata

10267
10887

XP_002309538
255761085
0.868105516

Populus trichocarpa

10268

XP_002324794
255761085
0.857314149

Populus trichocarpa

10269

40-60
AAS10176
41745611
1

Antirrhinum majus

10270
10888

554-574
XP_003530109
356522957
1

Glycine max

10271
10889

XP_003531653
356526090
0.95823389

Glycine max

10272
10890

XP_003627005
357513432
0.91646778

Medicago truncatula

10273
10891

XP_003597690
357454818
0.918854415

Medicago truncatula

10274
10892

590-610
XP_003524993
356512573
1

Glycine max

10275
10893

XP_002298892
255761085
0.832356389

Populus trichocarpa

10276

XP_003594520
357448488
0.781946073

Medicago truncatula

10277
10894

25-45
XP_003522716
356507930
1

Glycine max

10278
10895

XP_003603630
357466690
0.932464455

Medicago truncatula

10279
10896

560-580
XP_003530112
356522963
1

Glycine max

10280
10897

572-592
P00965

1

Phaseolus vulgaris

10281

XP_003544980
356553268
0.935393258

Glycine max

10282
10898

XP_003519325
356501016
0.935393258

Glycine max

10283
10899

ACU19484
255638343
0.926966292

Glycine max

10284
10900

XP_003519326
356501018
0.935393258

Glycine max

10285
10901

AAB61597
2213876
0.901685393

Hevea brasiliensis

10286
10902

P32289

0.901685393

Vigna aconitifolia

10287

ABW89460
159138920
0.904494382

Gossypium herbaceum

10288
10903

P04770

0.896067416

Phaseolus vulgaris

10289

ABW89461
159138922
0.901685393

Gossypium hirsutum

10290
10904

87-107
CAN73584
147820217
1

Vitis vinifera

10291

530-550
XP_003532788
356528393
1

Glycine max

10292
10905

516-536
XP_003537529
356538072
1

Glycine max

10293
10906

566-586
XP_003531653
356526090
1

Glycine max

10294
10907

560-580
XP_003539764
356542618
1

Glycine max

10295
10908

XP_002285176
225435326
0.894674556

Vitis vinifera

10296
10909

CAC84906
18076735
0.829585799

Zinnia violacea

10297
10910

828-848
XP_003539765
356542620
1

Glycine max

10298
10911

ptc-
557-577
XP_002298892
255761085
1

Populus trichocarpa

10299

miR166p

CAN73584
147820217
0.91943128

Vitis vinifera

10300

XP_002332526
255761085
0.94549763

Populus trichocarpa

10301

XP_002281868
225444032
0.91943128

Vitis vinifera

10302
10912

XP_003535078
356533042
0.892180095

Glycine max

10303
10913

XP_003546255
356555874
0.893364929

Glycine max

10304
10914

AAS66760
45479745
0.86492891

Nicotiana sylvestris

10305
10915

XP_003532788
356528393
0.849526066

Glycine max

10306
10916

XP_003524993
356512573
0.845971564

Glycine max

10307
10917

ACI13683
206572100
0.816350711

Malus × domestica
10308
10918

515-535
XP_003597690
357454818
1

Medicago truncatula

10309
10919

XP_003531652
356526088
0.918660287

Glycine max

10310
10920

XP_003530109
356522957
0.921052632

Glycine max

10311
10921

XP_002284003
225442500
0.897129187

Vitis vinifera

10312
10922

CBI36079
270253379
0.897129187

Vitis vinifera

10313

XP_002515977
255761086
0.901913876

Ricinus communis

10314

XP_003530112
356522963
0.916267943

Glycine max

10315
10923

XP_002284014
225442502
0.897129187

Vitis vinifera

10316
10924

ACI13685
206572104
0.89354067

Malus × domestica
10317
10925

XP_002304217
255761085
0.888755981

Populus trichocarpa

10318

560-580
XP_002285176
225435326
1

Vitis vinifera

10319
10926

CAN61612
147783603
0.981042654

Vitis vinifera

10320

XP_002529946
255761086
0.918246445

Ricinus communis

10321

XP_003538150
356539326
0.892180095

Glycine max

10322
10927

XP_003539764
356542618
0.895734597

Glycine max

10323
10928

ACI13684
206572102
0.888625592

Malus × domestica
10324
10929

XP_003539765
356542620
0.890995261

Glycine max

10325
10930

AAX19050
60327620
0.881516588

Populus trichocarpa

10326
10931

DAA05766
109729904
0.853080569

Lotus japonicus

10327

AAY33856
63115353
0.8507109

Gossypium barbadense

10328
10932

554-574
XP_003603630
357466690
1

Medicago truncatula

10329
10933

XP_003522716
356507930
0.943645084

Glycine max

10330
10934

XP_003526496
356515618
0.940047962

Glycine max

10331
10935

ACI13686
206572106
0.872901679

Malus × domestica
10332
10936

ADL36609
302398628
0.863309353

Malus × domestica
10333
10937

CBI20838
270231236
0.862110312

Vitis vinifera

10334

XP_002283717
225429913
0.862110312

Vitis vinifera

10335
10938

ACL51017
219879369
0.858513189

Citrus trifoliata

10336
10939

XP_002309538
255761085
0.868105516

Populus trichocarpa

10337

XP_002324794
255761085
0.857314149

Populus trichocarpa

10338

40-60
AAS10176
41745611
1

Antirrhinum majus

10339
10940

554-574
XP_003530109
356522957
1

Glycine max

10340
10941

XP_003531653
356526090
0.95823389

Glycine max

10341
10942

XP_003627005
357513432
0.91646778

Medicago truncatula

10342
10943

XP_003597690
357454818
0.918854415

Medicago truncatula

10343
10944

590-610
XP_003524993
356512573
1

Glycine max

10344
10945

XP_003594520
357448488
0.781946073

Medicago truncatula

10345
10946

25-45
XP_003522716
356507930
1

Glycine max

10346
10947

XP_003603630
357466690
0.932464455

Medicago truncatula

10347
10948

560-580
XP_003530112
356522963
1

Glycine max

10348
10949

530-550
XP_003532788
356528393
1

Glycine max

10349
10950

87-107
CAN73584
147820217
1

Vitis vinifera

10350

113-133
XP_003516553
356495373
1

Glycine max

10351
10951

XP_003537620
356538255
0.909756098

Glycine max

10352
10952

566-586
XP_003531653
356526090
1

Glycine max

10353
10953

560-580
XP_003539764
356542618
1

Glycine max

10354
10954

XP_002285176
225435326
0.894674556

Vitis vinifera

10355
10955

CAC84906
18076735
0.829585799

Zinnia violacea

10356
10956

828-848
XP_003539765
356542620
1

Glycine max

10357
10957

ptc-
368-388
XP_003550796
356565126
1

Glycine max

10358
10958

miRf10007-

XP_003528595
356519875
0.931982634

Glycine max

10359
10959

akr

XP_003609844
357479116
0.714905933

Medicago truncatula

10360
10960

ptc-
600-621
XP_003520116
356502619
1

Glycine max

10361
10961

miRf11396-

XP_002280702
225438602
0.856050955

Vitis vinifera

10362
10962

akr

EAZ28751
54398660
0.829299363

Oryza sativa

10363

Japonica Group

ptc-
88-108
NP_001236364
351726593
1

Glycine max

10364
10963

miRf12069-

akr

Example 4
Verification of Expression of miRNA Molecules Associated with Abiotic Stress

Following identification of small RNA molecules potentially involved in improvement of soybean abiotic stress tolerance and their target genes (mRNAs) using bioinformatics tools, as described in Example 4 above, the actual mRNA levels in an experiment are determined using reverse transcription assay followed by quantitative Real-Time PCR (qRT-PCR) analysis. RNA levels are compared between different tissues, developmental stages, growing conditions and/or genetic backgrounds incorporated in each experiment. A correlation analysis between mRNA levels in different experimental conditions/genetic backgrounds is applied and used as evidence for the role of the gene in the plant.

Methods

Root and leaf samples are freshly excised from soybean plants grown as described above on Murashige-Skoog (Duchefa). Experimental plants are grown either under optimal irrigation conditions, salt levels or temperatures to be used as a control group, or under stressful conditions of prolonged water deprivation, high salt concentrations and a heat shock treatment at a temperature higher than 34° C. to be used as stress-induced groups to assess the drought, salinity and heat shock tolerance, respectively, of control versus transgenic plants. Total RNA is extracted from the different tissues, using mirVana™ commercial kit (Ambion) following the protocol provided by the manufacturer. For measurement and verification of messenger RNA (mRNA) expression level of all genes, reverse transcription followed by quantitative real time PCR (qRT-PCR) is performed on total RNA extracted from each plant tissue (i.e., roots and leaves) from each experimental group as described above. To elaborate, reverse transcription is performed on 1 μg total RNA, using a miScript Reverse Transcriptase kit (Qiagen), following the protocol suggested by the manufacturer. Quantitative RT-PCR is performed on cDNA (0.1 ng/μl final concentration), using a miScript SYBR GREEN PCR (Qiagen) forward (based on the miR sequence itself) and reverse primers (supplied with the kit). All qRT-PCR reactions are performed in triplicates using an ABI7500 real-time PCR machine, following the recommended protocol for the machine. To normalize the expression level of miRNAs associated with enhanced abiotic stress tolerance between the different tissues and growing conditions of the soybean plants, normalizer miRNAs are selected and used for comparison. Normalizer miRNAs, which are miRNAs with unchanged expression level between tissues and growing conditions, are custom selected for each experiment. The normalization procedure consists of second-degree polynomial fitting to a reference data (which is the median vector of all the data—excluding outliers) as described by Rosenfeld et al (2008, Nat Biotechnol, 26(4):462-469). A summary of primers for the differential small RNA molecules that will be used in the qRT-PCR validation and analysis is presented in Table 11a below.

TABLE 11a

Primers of Differential miRNA Molecules for qRT-PCR Validation

Step.

Primer

Mir Name
Primer Sequence (SEQ ID NO:)
Length
Tm

ahy-miR3514-5p
TGGCAGGATTCTGTATTAACGGTGGA (10964)
26
59.6

aly-miR160c-3p
GCGTACAAGGAGCCAAGCATG (10965)
21
58.5

aly-miR396a-3p
GGCGTTCAATAAAGCTGTGGGAAG (10966)
24
58.7

aly-miR396b-3p
GCGCTCAAGAAAGCTGTGGGAAA (10967)
23
60.3

aly-miR831-5p
GGCAGAAGAGGTACAAGGAGATGAGA (10968)
26
59.2

aqc-miR159
GGCTTTGGACTGAAGGGAGCTCTA (10969)
24
59.8

ath-miR157a
TTTGGCTTGACAGAAGATAGAGAGCAC (10970)
27
58.8

ath-miR159b
GGCTTTGGATTGAAGGGAGCTCTT (10971)
24
59.0

ath-miR159c
GCTTTGGATTGAAGGGAGCTCCT (10972)
23
58.7

ath-miRf10068-akr
CACCGGTGGAGGAGTGAGAG (10973)
20
58.0

ath-miRf10148-akr
GGCGGTGGTGGAAAGATCAAGAT (10974)
23
59.1

ath-miRf10197-akr
CACTCGACCAAGGGGGTCGAGTGA (10975)
24
63.6

ath-miRf10209-akr
ATGGTGGTACTCGGCCAGGTGGT (10976)
23
63.5

ath-miRf10239-akr
GCCGCCTTGCATCAACTGAATC (10977)
22
59.2

ath-miRf10240-akr
GCATCGAAGGAGATGGAGGACG (10978)
22
59.0

ath-miRf10279-akr
ACTCAGCCTGGGGGTCGAG (10979)
19
59.7

ath-miRf10368-akr
GGCACTTGGGTGGTGCTGATTAT (10980)
23
59.3

ath-miRf10451-akr
GGCAAGAAGGAGGAACAACCTGTTG (10981)
25
60.0

ath-miRf10633-akr
TGGCGGTGGATACTTCTTGATCGG (10982)
24
60.5

ath-miRf10687-akr
GGCTTAGCTGAAGAAGCAGAGGAG (10983)
24
58.9

ath-miRf10701-akr
TGCAGTTCCTGGAGGTGGAGG (10984)
21
60.0

ath-miRf10702-akr
CGTGGGAGGACTCCAAGTGTG (10985)
21
58.9

ath-miRf10751-akr
GCCTTGTGGAGAGGAAGCAAGA (10986)
22
58.6

ath-miRf10763-akr
GCGGTGGTGAAGAAGCATGGTT (10987)
22
60.1

ath-miRf10924-akr
GCTGAGGCGTATCAGGAGGTAGT (10988)
23
59.4

ath-miRf11021-akr
GGCGAGGTTTGCGATGAGAAAGAG (10989)
24
60.2

ath-miRf11037-akr
GCTCATCGGAGAAACAGAGGAGC (10990)
23
59.2

ath-miRf11042-akr
GGAAGAGGCAGTGCATGGGTA (10991)
21
58.3

ath-miRf11045-akr
GGCTTTCTTGTGGAGGAAGCAAGAT (10992)
25
59.3

bdi-miR2508
GCATTGAGTGCAGCGTTGATGAAC (10993)
24
59.7

bna-miR2111b-5p
CATTTGGCTAATCTGCATCCTGAGGTTTA (10994)
29
59.1

bra-miR160a-3p
CGCGTATGAGGAGCCATGCATA (10995)
22
59.0

csi-miR162-5p
TGGAGGCAGCGGTTCATCGATC (10996)
22
61.1

csi-miR3946
GGCTTGTAGAGAAAGAGAAGAGAGCAC (10997)
27
58.8

csi-miR3948
TGGAGTGGGAGTGGGAGTAGGGTG (10998)
24
62.6

ctr-miR171
GCTTGAGCCGCGTCAATATCTCC (10999)
23
60.1

far-miR1134
GCCGACAACAACAACAAGAAGAAGAG (11000)
26
58.9

ghr-miR2950
TGGTGTGCAGGGGGTGGAATA (11001)
21
59.6

gma-miR1507a
TGGCTCTCATTCCATACATCGTCTGA (11002)
26
59.2

gma-miR1524
CGAGTCCGAGGAAGGAACTCC (11003)
21
58.2

gma-miR156g
TTTGGCACAGAAGATAGAGAGCACAG (11004)
26
58.7

gma-miR157c
TGGCTGACAGAAGACTAGAGAGCAC (11005)
25
59.6

gma-miR159a-3p
TGGCTTTGGATTGAAGGGAGCTCTA (11006)
25
59.5

gma-miR159d
GCAGCTGCTTAGCTATGGATCCC (11007)
23
59.3

gma-miR2119
GGCTCAAAGGGAGTTGTAGGGGAA (11008)
24
60.0

gma-miR396d
GCAAGAAAGCTGTGGGAGAATATGGC (11009)
26
60.2

gma-miR4371b
AAGTGATGACGTGGTAGACGGAGT (11010)
24
59.3

gma-miR4376-5p
TACGCAGGAGAGATGACGCTGT (11011)
22
59.6

gma-miR4412-3p
AGTGGCGTAGATCCCCACAAC (11012)
21
58.4

gma-miR4416a
ACGGGTCGCTCTCACCTAGG (11013)
20
59.5

gma-miR482a-3p
TCTTCCCAATTCCGCCCATTCCTA (11014)
24
59.6

gma-miR482b-5p
GCTATGGGGGGATTGGGAAGGAAT (11015)
24
59.9

gso-miR169g*
TCGGCAAGTTGGCCTTGGCT (11016)
20
61.5

gso-miR482a
GGCTCTTCCCTACACCTCCCATAC (11017)
24
59.7

iba-miR157
CATTTGGCTTGACAGAAGATAGAGAGCAT (11018)
29
58.9

mdm-miR482a-5p
CGGAATGGGCTGTTTGGGAACA (11019)
22
59.7

mtr-miR2119
GCTCAAAGGGAGGTGTGGAGTAG (11020)
23
58.5

osa-miR159e
GCATTGGATTGAAGGGAGCTCCT (11021)
23
58.8

osa-miR159f
GGCCTTGGATTGAAGGGAGCTCTA (11022)
24
59.9

osa-miR162a
GGCTCGATAAACCTCTGCATCCAG (11023)
24
59.3

osa-miR1846e
CAACGAGGAGGCCGGGACCA (11024)
20
62.8

osa-miR1850.1
GCTGGAAAGTTGGGAGATTGGGG (11025)
23
59.6

osa-miR1858a
GAGAGGAGGACGGAGTGGGGC (11026)
21
62.2

osa-miR1869
GCTGAGAACAATAGGCATGGGAGGTA (11027)
26
60.0

osa-miR1874-3p
GCTATGGATGGAGGTGTAACCCGATG (11028)
26
60.6

osa-miR1879
CGTGTTTGGTTTAGGGATGAGGTGG (11029)
25
59.6

osa-miR1881
GCAATGTTATTGTAGCGTGGTGGTGT (11030)
26
60.1

osa-miR2055
GGCTTTCCTTGGGAAGGTGGTTTC (11031)
24
60.0

osa-miR2104
GCGGCGAGGGGATGCGAGCG (11032)
20
67.4

osa-miRf10105-akr
TTGGCCTCGTCGAAGAAGGAGA (11033)
22
59.5

osa-miRf10151-akr
GGCTGGCTATATTTTGGGACGGAG (11034)
24
59.3

osa-miRf10362-akr
GCTGGAGGATGCGACGGTGCT (11035)
21
63.6

osa-miRf10839-akr
CCCTGTGACGTTGGTGAAGGTG (11036)
22
59.7

osa-miRf10849-akr
TGGACTGTTTGGGGGAGCTTCT (11037)
22
59.6

osa-miRf11013-akr
GGTTTGCCGGAGTTGGAGGAGA (11038)
22
60.6

osa-miRf11341-akr
CGCGCCGACGATGACGGTGGAGT (11039)
23
67.4

osa-miRf11352-akr
GCAGGGATTTTGGAAGGAGGTGACA (11040)
25
60.8

osa-miRf11355-akr
GGTGGAGGTGGAGCTGTGCCAAA (11041)
23
63.2

osa-miRf11415-akr
GAGAGCAGGATGCAGCCAAGG (11042)
21
59.6

osa-miRf11595-akr
CCATCGGTGTTGGAGGTGGC (11043)
20
59.8

osa-miRf11649-akr
AAACCGTGCAAAGGAGGTCCC (11044)
21
59.4

osa-miRf11829-akr
ACGCGGAGGAGGTGGTGTTCT (11045)
21
62.0

osa-miRf11996-akr
GCGTCTTATAACCTGAAACGGGGG (11046)
24
59.5

pab-miR3711
TGGCGCTAGAAGGAGGGCCT (11047)
20
61.6

ppt-miR1220a
GCTTCCGGTGGTGAGGAAGATAG (11048)
23
58.6

ppt-miR166m
GCTCGGACCAGGCATCATTCCTT (11049)
23
61.0

ppt-miR533b-5p
GAGCTGTCCAGGCTGTGAGGG (11050)
21
61.0

ppt-miR895
GCGTAGCTTAGCGAGGTGTTGGTA (11051)
24
60.7

psi-miR159
GCCTTGGATTGAAGGGAGCTCCA (11052)
23
60.6

pta-miR156a
TTTGGCCAGAAGATAGAGAGCACATC (11053)
26
58.5

pta-miR156b
TTGGCCAGAAGATAGAGAGCACAAC (11054)
25
58.6

pta-miR166c
CCGGACCAGGCTTCATCCCAG (11055)
21
61.1

ptc-miR166p
TCGGACCAGGCTCCATTCCTT (11056)
21
59.4

ptc-miRf10007-akr
GCCATTGACAGGGAAACTCACCA (11057)
23
59.2

ptc-miRf10132-akr
TTGGCGGTGATTGAACGGAGGGT (11058)
23
62.7

ptc-miRf10148-akr
TGGTGCACCTGGTGGTGGAG (11059)
20
60.8

ptc-miRf10226-akr
TCCTTTGGGGAGATGGAGAGCTT (11060)
23
58.9

ptc-miRf10271-akr
TGGCTTGGATTGAAGGGAGCTCTAA (11061)
25
59.5

ptc-miRf10300-akr
GGCTTTGGAAAGCAAGTGAGGTG (11062)
23
58.7

ptc-miRf10522-akr
TTGGGGAGCTGGACTCTGGA (11063)
20
58.6

ptc-miRf10619-akr
GTTGGGCTTGCTGCTGGAGGA (11064)
21
61.5

ptc-miRf10734-akr
GCCATCTAGGTGGTGGTCCAGTG (11065)
23
60.7

ptc-miRf10976-akr
TGGGAACGTGGCTGTGGCTA (11066)
20
60.3

ptc-miRf10985-akr
TGGCCAGAAGATAGAGAGCACTGA (11067)
24
58.8

ptc-miRf11018-akr
CCTGCAAACCTAAGGGAGCGG (11068)
21
59.6

ptc-miRf11079-akr
AAGATGGAGAAGCAGGGCACGTGC (11069)
24
63.4

ptc-miRf11315-akr
GCCAACTTAGAGTTGGGGGTGG (11070)
22
59.2

ptc-miRf11324-akr
CTTGTCGCAGGAGAGATGGCGCT (11071)
23
63.1

ptc-miRf11396-akr
GCCAAGGCTCTGATACCATGTCAA (11072)
24
58.9

ptc-miRf11669-akr
GGCCAAGGCTCTGATACCATGTT (11073)
23
58.8

ptc-miRf11757-akr
CCTTGGTGAATGGTTGGGAGGAAT (11074)
24
58.7

ptc-miRf11844-akr
CCCAACTTGGAGGTGGGTGTGG (11075)
22
61.4

ptc-miRf11847-akr
GCGAAAGTGTGGAGAAGGTTGCC (11076)
23
60.6

ptc-miRf11855-akr
GGCAGAGCATGGATGGAGCTA (11077)
21
58.2

ptc-miRf11953-akr
GGCGTAATCTGCATCCTGAGGTT (11078)
23
58.9

ptc-miRf12069-akr
GGAGGGGCTGCAAGACCCAAG (11079)
21
61.5

ptc-miRf12389-akr
GTCGACCTGGCGAGTCAACCGGG (11080)
23
65.3

sbi-miR159a
GGCTTTGGATTGAAGGGAGCTCTG (11081)
24
59.6

smo-miR1103-3p
GCTGGAAAAAGGAGGTGCATTCTTGT (11082)
26
60.0

smo-miR156b
TGGCCTGACAGAAGATAGAGAGCAC (11083)
25
59.7

tae-miR2003
CGGTTGGGCTGTATGATGGCGA (11084)
22
61.3

vvi-miR2111-5p
TGGCTAATCTGCATCCTGAGGTCTA (11085)
25
58.7

vvi-miR394b
GCTTGGCATTCTGTCCACCTCC (11086)
22
59.9

zma-miR167u
GGCTGAAGCTGCCACATGATCTG (11087)
23
60.2

zma-miR396b-3p
GGCTTCCACAGCTTTCTTGAACTG (11088)
24
58.5

zma-miR398a-5p
TGTGTTCTCAGGTCGCCCCCG (11089)
21
62.9

zma-miR482-5p
TGGCTCTTCCTTGTTCCTCCCATT (11090)
24
59.7

Alternative RT-PCR Validation Method of Selected microRNAs of the Invention

A novel microRNA quantification method has been applied using stem-loop RT followed by PCR analysis (Chen C, Ridzon D A, Broomer A J, Zhou Z, Lee D H, Nguyen J T, Barbisin M, Xu N L, Mahuvakar V R, Andersen M R, Lao K Q, Livak K J, Guegler K J. 2005, Nucleic Acids Res 33(20):e179; Varkonyi-Gasic E, Wu R, Wood M, Walton E F, Hellens R P. 2007, Plant Methods 3:12). This highly accurate method allows the detection of less abundant miRNAs. In this method, stem-loop RT primers are used, which provide higher specificity and efficiency to the reverse transcription process. While the conventional method relies on polyadenylated (poly (A)) tail and thus becomes sensitive to methylation because of the susceptibility of the enzymes involved, in this novel method the reverse transcription step is transcript-specific and insensitive to methylation. Reverse transcriptase reactions contained RNA samples including purified total RNA, 50 nM stem-loop RT primer (50-51 nucleotide long, see Table 11b, synthesized by Sigma), and using the SuperScript II reverse transcriptase (Invitrogen). A mix of up to 12 stem-loop RT primers may be used in each reaction, and the forward primers are such that the last 6 nucleotides are replaced with a GC rich sequence. For the PCR step, each miRNA has a custom forward primer (18-24 nucleotide long) and a universal stem loop reverse primer (5′-GTGCAGGGTCCGAGGT-3′-SEQ ID NO: 11617). Table 11b below lists the primers used for PCR validation using this method. Note, SL-RT stands for stem loop reverse transcription primer, and SL-F stands for stem loop forward primer.

TABLE 11b

Stem Loop Reverse Transcriptase Primers for RT-PCR Validation of

Differential Mirs under Abiotic Stress.

Forward

Forward Primer
Primer

Mir Name
Stem Loop Primer (SEQ ID NO:)
(SEQ ID NO:)
Length

ahy-miR3514-5p
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGAGGATTCTGTAT
22

GCACTGGATACGACTCCACC (11618)
TAAC (11619)

aly-miR396a-3p
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGGTTCAATAAAG
21

GCACTGGATACGACCTTCCC (11620)
CTGT (11621)

aly-miR396b-3p
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGGCTCAAGAAAG
21

GCACTGGATACGACTTTCCC (11622)
CTGT (11623)

aly-miR831-5p
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGAGAAGAGGTAC
23

GCACTGGATACGACTCTCAT (11624)
AAGGAG (11625)

ath-miRf10197-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGCTCACTCGACCAAGG
21

akr
GCACTGGATACGACTCACTCG (11626)
GGGT (11627)

ath-miRf10279-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
TTCCACTCAGCCTGGGGG
21

akr
GCACTGGATACGACATCACT (11628)
TCG (11629)

ath-miRf10687-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGTTAGCTGAAGA
21

akr
GCACTGGATACGACCTCCTC (11630)
AGCA (11631)

gma-miRf10687-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
GAGCTTAGCCGCAGAGG
19

akr-homolog
GCACTGGATACGACCTCCTC (11632)
CA (11633)

ath-miRf11021-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGAGGTTTGCGATG
19

akr
GCACTGGATACGACCTCTTT (11634)
AG (11635)

ath-miRf11045-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGTTTCTTGTGGAG
22

akr
GCACTGGATACGACATCTTG (11636)
GAAG (11637)

csi-miR162-5p
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGTCTGGAGGCAGCGGTT
20

GCACTGGATACGACGATCGA (11638)
CA (11639)

far-miR1134
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGCGCCGACAACAACAA
23

GCACTGGATACGACCTCTTC (11640)
CAAGAA (11641)

gma-miR159d
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGAGCTGCTTAGCT
21

GCACTGGATACGACGGGATC (11642)
ATG (11643)

gma-miR396d
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGCGCAAGAAAGCTGTG
23

GCACTGGATACGACGCCATA (11644)
GGAGAA (11645)

gma-miR4376-5p
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
GGCCGGTACGCAGGAGA
22

GCACTGGATACGACACAGCG (11646)
GATGA (11647)

gma-miR4412-3p
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
TTCCAGTGGCGTAGATCC
19

GCACTGGATACGACGTTGTG (11648)
C (11649)

gma-miR4416a
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCACGGGTCGCTCTCA
18

GCACTGGATACGACCCTAGG (11650)
(11651)

gma-miR482a-3p
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGTCTCTTCCCAATTCCG
22

GCACTGGATACGACTAGGAA (11652)
CCCA (11653)

gso-miR169g*
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
GTTACTCGGCAAGTTGGC
18

GCACTGGATACGACAGCCAAG (11654)
(11655)

mtr-miR2119
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGTCAAAGGGAGG
21

GCACTGGATACGACCTACTC (11656)
TGTG (11657)

osa-miR1874-3p
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGTATGGATGGAG
24

GCACTGGATACGACCATCGG (11658)
GTGTAAC (11659)

osa-miRf10105-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CCGGTTGGCCTCGTCGAA
20

akr
GCACTGGATACGACTCTCCT (11660)
GA (11661)

osa-miRf10151-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGTGGCTATATTTT
21

akr
GCACTGGATACGACCTCCGT (11662)
GGG (11663)

osa-miRf10362-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGATGCTGGAGGATGCG
19

akr
GCACTGGATACGACAGCACC (11664)
AC (11665)

osa-miRf10839-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCCCTGTGACGTTGGT
19

akr
GCACTGGATACGACCACCTT (11666)
G (11667)

osa-miRf11649-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
GCGCAAACCGTGCAAAG
19

akr
GCACTGGATACGACGGGACC (11668)
GA (11669)

pab-miR3711
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
GGCCCTGGCGCTAGAAG
19

GCACTGGATACGACAGGCCC (11670)
GA (11671)

ppt-miR533b-5p
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGAGCTGTCCAGGCT
19

GCACTGGATACGACCCCTCA (11672)
G (11673)

ppt-miR895
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGGTAGCTTAGCG
22

GCACTGGATACGACTACCAA (11674)
AGGTG (11675)

ptc-miRf10300-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGTTTGGAAAGCA
20

akr
GCACTGGATACGACCACCTC (11676)
AGT (11677)

ptc-miRf10522-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGCGCTTGGGGAGCTGG
19

akr
GCACTGGATACGACTCCAGA (11678)
AC (11679)

ptc-miRf10619-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGTTGGGCTTGCTGC
19

akr
GCACTGGATACGACTCCTCC (11680)
T (11681)

ptc-miRf11855-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGCAGAGCATGGA
19

akr
GCACTGGATACGACTAGCTC (11682)
TG (11683)

ptc-miRf12069-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGTCGGAGGGGCTGCAA
19

akr
GCACTGGATACGACCTTGGG (11684)
GA (11685)

smo-miR1103-3p
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCTGGAAAAAGGAGG
22

GCACTGGATACGACACAAGA (11686)
TGCAT (11687)

zma-miR396b-3p
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGGTTCAATAAAG
21

GCACTGGATACGACTTTCCC (11688)
CTGT (11689)

zma-miR482-5p
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGTGGGAGATGAA
19

GCACTGGATACGACAAGGCT (11690)
GG (11691)

aly-miR160c-3p
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
GAATCGCGTACAAGGAG
20

GCACTGGATACGACCATGCT (11692)
CCA (11693)

aqc-miR159
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGTTTGGACTGAA
21

GCACTGGATACGACTAGAGC (11694)
GGGA (11695)

ath-miR157a
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGTTGACAGAAGA
21

GCACTGGATACGACGTGCTC (11696)
TAGA (11697)

ath-miR159b
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGTTTGGATTGAAG
21

GCACTGGATACGACAAGAGC (11698)
GGA (11699)

ath-miR159c
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGTTTGGATTGAAG
21

GCACTGGATACGACAGGAGC (11700)
GGA (11701)

ath-miRf10068-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
GGCCCACCGGTGGAGGA
18

akr
GCACTGGATACGACCTCTCA (11702)
G (11703)

ath-miRf10148-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGGGTGGTGGAAA
20

akr
GCACTGGATACGACATCTTG (11704)
GAT (11705)

ath-miRf10209-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGTCATGGTGGTACTCGG
21

akr
GCACTGGATACGACACCACC (11706)
CCA (11707)

ath-miRf10239-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCCGCCTTGCATCAAC (
18

akr
GCACTGGATACGACGATTCA (11708)
11709)

ath-miRf10240-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGATCGAAGGAGA
20

akr
GCACTGGATACGACCGTCCT (11710)
TGG (11711)

ath-miRf10368-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
AAGGCCTACTTGGGTGGT
21

akr
GCACTGGATACGACATAATC (11712)
GCT (11713)

ath-miRf10451-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGAAGAAGGAGGA
22

akr
GCACTGGATACGACCAACAG (11714)
ACAAC (11715)

ath-miRf10633-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCTGGCGGTGGATACT
22

akr
GCACTGGATACGACCCGATC (11716)
TCTT (11717)

ath-miRf10701-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGCGCTGCAGTTCCTGGA
21

akr
GCACTGGATACGACTCCTCC (11718)
GGT (11719)

ath-miRf10702-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGTGGGAGGACTCC
18

akr
GCACTGGATACGACCACACT (11720)
A (11721)

ath-miRf10751-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGCTTGTGGAGAG
20

akr
GCACTGGATACGACTCTTGC (11722)
GAA (11723)

ath-miRf10763-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGCGCGGTGGTGAAGAA
19

akr
GCACTGGATACGACAACCAT (11724)
GC (11725)

ath-miRf10924-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGTAGGTGAGGCGTATC
21

akr
GCACTGGATACGACACTACC (11726)
AGGA (11727)

ath-miRf11037-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGTCATCGGAGAA
21

akr
GCACTGGATACGACGCTCCT (11728)
ACAG (11729)

ath-miRf11042-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
AATCCTGGAAGAGGCAG
20

akr
GCACTGGATACGACTACCCAT (11730)
TGC (11731)

bdi-miR2508
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCATTGAGTGCAGCGT
20

GCACTGGATACGACGTTCAT (11732)
TG (11733)

bna-miR2111b-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGTAATCTGCATCC
21

5p
GCACTGGATACGACTAAACC (11734)
TGA (11735)

bra-miR160a-3p
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
GAATCGCGTATGAGGAG
20

GCACTGGATACGACTATGCA (11736)
CCA (11737)

csi-miR3946
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGTTGTAGAGAAA
24

GCACTGGATACGACGTGCTC (11738)
GAGAAGA (11739)

csi-miR3948
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCTGGAGTGGGAGTG
22

GCACTGGATACGACCACCCT (11740)
GGAGT (11741)

ctr-miR171
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
AATCCTTTGAGCCGCGTC
21

GCACTGGATACGACGGAGAT (11742)
AAT (11743)

ghr-miR2950
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGTCTGGTGTGCAGGGG
19

GCACTGGATACGACTATTCC (11744)
GT (11745)

gma-miR1507a
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGTCTCATTCCATA
22

GCACTGGATACGACTCAGAC (11746)
CATC (11747)

gma-miR1524
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
GCGCCGAGTCCGAGGAA
19

GCACTGGATACGACGGAGTT (11748)
GG (11749)

gma-miR156g
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
GCGGCGGACAGAAGATA
21

GCACTGGATACGACCTGTGC (11750)
GAGA (11751)

gma-miR157c
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGTGACAGAAGAC
21

GCACTGGATACGACGTGCTC (11752)
TAGA (11753)

gma-miR159a-3p
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGTTTGGATTGAAG
21

GCACTGGATACGACTAGAGC (11754)
GGA (11755)

gma-miR2119
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGTCAAAGGGAGT
21

GCACTGGATACGACTTCCCC (11756)
TGTA (11757)

gma-miR4371b
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGAAGTGATGACG
24

GCACTGGATACGACACTCCG (11758)
TGGTAGA (11759)

gma-miR482b-5p
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCTATGGGGGGATTG
20

GCACTGGATACGACATTCCT (11760)
GGA (11761)

gso-miR482a
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGTCTTCCCTACAC
21

GCACTGGATACGACGTATGG (11762)
CTC (11763)

iba-miR157
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGTTGACAGAAGA
21

GCACTGGATACGACATGCTC (11764)
TAGA (11765)

mdm-miR482a-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGAATGGGCTGTTT
19

5p
GCACTGGATACGACTGTTCC (11766)
G (11767)

osa-miR159e
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGATTGGATTGAA
21

GCACTGGATACGACAGGAGC (11768)
GGGA (11769)

osa-miR159f
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
AAGGCCTCTTGGATTGAA
22

GCACTGGATACGACTAGAGC (11770)
GGGA (11771)

osa-miR162a
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGTCGATAAACCTC
21

GCACTGGATACGACCTGGAT (11772)
TGC (11773)

osa-miR1846e
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGTCCAACGAGGAGGCC
18

GCACTGGATACGACTGGTCC (11774)
G (11775)

osa-miR1850.1
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGTGGAAAGTTGG
21

GCACTGGATACGACCCCCAA (11776)
GAGA (11777)

osa-miR1858a
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGAGAGGAGGACGG
19

GCACTGGATACGACGCCCCA (11778)
AG (11779)

osa-miR1869
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGCGCTGAGAACAATAG
23

GCACTGGATACGACTACCTC (11780)
GCATGG (11781)

osa-miR1879
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGCGCGTGTTTGGTTTAG
23

GCACTGGATACGACCCACCT (11782)
GGATG (11783)

osa-miR1881
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGAATGTTATTGTA
24

GCACTGGATACGACACACCA (11784)
GCGTGG (11785)

osa-miR2055
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
AAGGCCTTTTCCTTGGGA
22

GCACTGGATACGACGAAACC (11786)
AGGT (11787)

osa-miR2104
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
TTAGCGGCGAGGGGATG
19

GCACTGGATACGACCACGCT (11788)
CG (11789)

osa-miRf10849-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCTGGACTGTTTGGGG
20

akr
GCACTGGATACGACAGAAGC (11790)
GA (11791)

osa-miRf11013-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
TAGGGTTTGCCGGAGTTG
19

akr
GCACTGGATACGACTCTCCT (11792)
G (11793)

osa-miRf11341-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
TAGCGCGCCGACGATGA
19

akr
GCACTGGATACGACACTCCAC (11794)
CG (11795)

osa-miRf11352-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGCGCAGGGATTTTGGA
22

akr
GCACTGGATACGACTGTCAC (11796)
AGGAG (11797)

osa-miRf11355-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGTCGGTGGAGGTGGAG
21

akr
GCACTGGATACGACTTTGGC (11798
CTGT (11799)

osa-miRf11415-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGAGAGCAGGATGC
19

akr
GCACTGGATACGACCCTTGG (11800)
AG (11801)

osa-miRf11595-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGCGCCATCGGTGTTGGA
18

akr
GCACTGGATACGACGCCACC (11802)
(11803)

osa-miRf11829-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGTCACGCGGAGGAGGT
19

akr
GCACTGGATACGACAGAACA (11804)
GG (11805)

osa-miRf11996-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGGTCTTATAACCT
22

akr
GCACTGGATACGACCCCCCG (11806)
GAAA (11807)

ppt-miR1220a
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
GCGCTTCCGGTGGTGAGG
19

GCACTGGATACGACCTATCT (11808)
A (11809)

ppt-miR166m
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCTCGGACCAGGCAT
19

GCACTGGATACGACAAGGAA (11810)
CA (11811)

psi-miR159
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
AAGGCCTCTTGGATTGAA
22

GCACTGGATACGACTGGAGC (11812)
GGGA (11813)

pta-miR156a
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGCAGAAGATAGA
20

GCACTGGATACGACGATGTG (11814)
GAG (11815)

pta-miR156b
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGCAGAAGATAGA
20

GCACTGGATACGACGTTGTG (11816)
GAG (11817)

pta-miR166c
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGTCCCGGACCAGGCTTC
19

GCACTGGATACGACCTGGGA (11818)
A (11819)

ptc-miR166p
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGTCTCGGACCAGGCTCC
19

GCACTGGATACGACAAGGAA (11820)
A (11821)

ptc-miRf10007-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGCATTGACAGGG
21

akr
GCACTGGATACGACTGGTGA (11822)
AAAC (11823)

ptc-miRf10132-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGTCTTGGCGGTGATTGA
21

akr
GCACTGGATACGACACCCTC (11824)
ACG (11825)

ptc-miRf10148-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCTGGTGCACCTGGTG
18

akr
GCACTGGATACGACCTCCAC (11826)
(11827)

ptc-miRf10226-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
AATCCTTCCTTTGGGGAG
23

akr
GCACTGGATACGACAAGCTC (11828)
ATGGA (11829)

ptc-miRf10271-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGTTGGATTGAAG
21

akr
GCACTGGATACGACTTAGAG (11830)
GGAG (11831)

ptc-miRf10734-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGCATCTAGGTGGT
21

akr
GCACTGGATACGACCACTGG (11832)
GGT (11833)

ptc-miRf10976-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCTGGGAACGTGGCT
18

akr
GCACTGGATACGACTAGCCA (11834)
G (11835)

ptc-miRf10985-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGCAGAAGATAGA
20

akr
GCACTGGATACGACTCAGTG (11836)
GAG (11837)

ptc-miRf11018-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGCTGCAAACCTA
20

akr
GCACTGGATACGACCCGCTC (11838)
AGG (11839)

ptc-miRf11079-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCAAGATGGAGAAGC
22

akr
GCACTGGATACGACGCACGT (11840)
AGGGC (11841)

ptc-miRf11315-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGCCGATCAACTTAGAGT
21

akr
GCACTGGATACGACCCACCC (11842)
TGG (11843)

ptc-miRf11324-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCCTTGTCGCAGGAG
21

akr
GCACTGGATACGACAGCGCC (11844)
AGAT (11845)

ptc-miRf11396-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGCGCCAAGGCTCTGATA
21

akr
GCACTGGATACGACTTGACA (11846)
CCA (11847)

ptc-miRf11669-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGCAAGGCTCTGA
20

akr
GCACTGGATACGACAACATG (11848)
TAC (11849)

ptc-miRf11757-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCCTTGGTGAATGGTT
21

akr
GCACTGGATACGACATTCCT (11850)
GGG (11851)

ptc-miRf11844-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCCCCAACTTGGAGGT
20

akr
GCACTGGATACGACCCACAC (11852)
GG (11853)

ptc-miRf11847-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGGAAAGTGTGGA
21

akr
GCACTGGATACGACGGCAAC (11854)
GAAG (11855)

ptc-miRf11953-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGGTAATCTGCATC
20

akr
GCACTGGATACGACAACCTC (11856)
CT (11857)

ptc-miRf12389-
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
TTCCGTCGACCTGGCGAG
21

akr
GCACTGGATACGACCCCGGT (11858)
TCA (11859)

sbi-miR159a
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGTTTGGATTGAAG
21

GCACTGGATACGACCAGAGC (11860)
GGA (11861)

smo-miR156b
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGCTGACAGAAGA
21

GCACTGGATACGACGTGCTC (11862)
TAGA (11863)

tae-miR2003
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
AACCGGTTGGGCTGTATG
19

GCACTGGATACGACTCGCCA (11864)
A (11865)

vvi-miR2111-5p
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGTAATCTGCATCC
21

GCACTGGATACGACTAGACC (11866)
TGA (11867)

vvi-miR394b
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGTTGGCATTCTGT
20

GCACTGGATACGACGGAGGT (11868)
CC (11869)

zma-miR167u
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CCGGGGTGAAGCTGCCA
20

GCACTGGATACGACCAGATC (11870)
CAT (11871)

zma-miR398a-5p
GTCGTATCCAGTGCAGGGTCCGAGGTATTC
CGGCGGGGCGAACTGAG
19

GCACTGGATACGACCATGTG (11872)
AA (11873)

Example 5
Results of RT-PCR Validation of Selected miRNAs of the Invention

An RT-PCR analysis was run on selected microRNAs of the invention, using the stem-loop RT primers as described in Table 11b in Example 4 above. Total RNA was extracted from leaf tissues of soybean plants grown as described above, and was used as a template for RT-PCR analysis. Expression level and directionality of several up-regulated and down-regulated microRNAs that were found to be differential on the microarray analysis were verified. Results are summarized in Table 12 below.

TABLE 12

Summary of RT-PCR Verification Results on Selected miRNAs using

Stem Loop RT (Alternative) Method

Trait
Mir Name
p-Value
Fold Change

Drought
gma-miR4376-5p
8.50E−03
2.38 (−)

zma-miR396b-3p
4.60E−03
1.41 (−)

aly-miR396b-3p
3.80E−06
3.48 (−)

gma-miR156g
5.40E−02
1.48 (+)

gma-miRf10687-akr-homolog
7.80E−02
1.95 (+)

Salt
gma-miR159d
5.60E−05
3.35 (−)

aly-miR396b-3p
3.80E−07
5.45 (−)

gma-miR4416a
5.20E−04
2.58 (−)

aly-miR396a-3p
1.20E−08
13.50 (−)

zma-miR396b-3p
4.90E−05
9.58 (−)

Heat Shock
gma-miR4412-3p
1.50E−03
2.31 (−)

csi-miR162-5p
2.60E−03
1.86 (−)

ath-miRf10279-akr
2.40E−02
1.40 (−)

Example 6
Generation of Transgenic Plants

Gene Cloning Strategies for miRNA Molecules and Creation of Binary Vectors for Expression in Plants

The best validated miRNA sequences are cloned into pORE-E1 binary vectors (FIG. 1) for the generation of transgenic plants. The full-length precursor sequence comprising of the hairpin sequence of each selected miRNA, is synthesized by Genscript (USA). The resulting clone is digested with appropriate restriction enzymes and inserted into the Multi Cloning Site (MCS) of a similarly digested binary vector through ligation using T4 DNA ligase enzyme (Promega, Madison, Wis., USA).

Example 7
Generation of Transgenic Model Plants Expressing Abiotic Stress Associated miRNAs

Arabidopsis thaliana Transformation Protocol

Arabidoposis thaliana transformation is performed using the floral dip procedure following a slightly modified version of the published protocol (Clough and Bent, 1998, Plant J 16(6): 735-43; and Desfeux et al., 2000, Plant Physiol 123(3): 895-904). Briefly, T0 Plants are planted in small pots filled with soil. The pots are covered with aluminum foil and a plastic dome, kept at 4° C. for 3-4 days, then uncovered and incubated in a growth chamber at 24° C. under 16 hr light:8 hr dark cycles. A week prior to transformation all individual flowering stems are removed to allow for growth of multiple flowering stems instead. A single colony of Agrobacterium (GV3101) carrying the binary vectors (pORE-E1 or pORE-E3, see FIGS. 1 and 3, respectively), harboring the selected miRNA hairpin sequences with additional flanking sequences both upstream and downstream of it, is cultured in LB medium supplemented with kanamycin (50 mg/L) and gentamycin (25 mg/L). Three days prior to transformation, each culture is incubated at 28° C. for 48 hrs, shaking at 180 rpm. The starter culture is split the day before transformation into two cultures, which are allowed to grow further at 28° C. for 24 hours at 180 rpm. Pellets containing the agrobacterium cells are obtained by centrifugation of the cultures at 5000 rpm for 15 minutes. The pellets are re-suspended in an infiltration medium (10 mM MgCl₂, 5% sucrose, 0.044 μM BAP (Sigma) and 0.03% Tween 20) prepared with double-distilled water.

Transformation of T0 plants is performed by inverting each plant into the agrobacterium suspension, keeping the flowering stem submerged for 5 minutes. Following inoculation, each plant is blotted dry for 5 minutes on both sides, and placed sideways on a fresh covered tray for 24 hours at 22° C. Transformed (transgenic) plants are then uncovered and transferred to a greenhouse for recovery and maturation. The transgenic T0 plants are grown in the greenhouse for 3-5 weeks until the seeds are ready, which are then harvested from plants and kept at room temperature until sowing.

Tomato (Solanum lycopersicum) Transformation Protocol

M82 tomato (Solanum lycopersicum) transformation is performed using a slightly modified protocol described previously (McCormick 1991, Plant Tissue Culture Manual, Vol. B6, Lindsey, K., ed. Dordrecht, The Netherlands: Kluwer Academic Publishers, pp. 1-9). Briefly, seeds are surface-sterilized 10 days prior to transformation by soaking and shaking in 70% ethanol followed by a 3% bleach solution. After washing, seeds are sown and allowed to grow into seedlings. Cotyledons are cut and treated with an agrobacterium (GV3101, carrying the binary vector pORE-E2, see FIG. 2) solution using a gentle agitation, dried and left on feeder medium for two days in the dark. Then, cotyledons are transferred to a first selection medium (Jones I) for two weeks, followed by a two-week incubation in a second selection medium (Jones II) for callus initiation. Cotyledons are transferred every two weeks to a fresh selection medium (Jones II) until plantlet forms. Plantlets with active meristems are separated from the callus and transferred to a third selection medium (Jones III), and transferred into a rooting medium. Once roots form, the plantlets are planted in soil.

Transgenic Arabidopsis Plants Over-Expressing Selected microRNA Sequences

Several microRNAs of the invention were selected for over-expression in both Arabidopsis. Transformation protocols are followed as described above for each plant, and agrobacterium is utilized, carrying one of three binary vectors: pORE-E1, pORE-E2, or pORE-E3.

Arabidopsis transgenic plants were created with osa-miRf11996-akr of the invention. This miRNA was downregulated under drought and salinity stresses compared to optimal conditions. Thus, the researchers tested the effects of modifying its expression level in model Arabidopsis plants. Four transgenic plants over-expressing osa-miRf11996-akr (in binary vector pORE-E3) were created and compared to control Columbia plants. Transgenic and control plants were grown under 16 h light:8 h dark regime at 22° C. in controlled growth rooms until seedlings were four weeks old. Next, plants were divided into two groups: control plants were irrigated with tap water twice a week and treated plants were either subjected to drought and received no irrigation for 5 days, or irrigated with 300 mM NaCl solution for 10 days for salinity stress induction. At the end of each treatment, plants were harvested and dry weight was recorded.

An ANOVA test was applied for statistical analysis of the data and results are summarized in Table 13 below. Control plants' dry weight was averaged and recorded as 100%, to be used as a reference for comparison to the average of each treated strain for each condition. Interestingly, the dry weight of all transgenic strains over-expressing osa-miRf11996-akr was significantly (p<1.0E-6) decreased compared to wild type control plants under all conditions. These results correlate with the fact that osa-miRf11996-akr was shown to be down-regulated under various abiotic stresses (e.g., Tables 2 and 4), thus indicating that its expression level needs to be decreased to improve a plant's tolerance to abiotic stress. Accordingly, the researchers also down-regulate osa-miRf11996-akr in Arabidopsis plants by using target mimic, as described and explained in Example 12 below.

TABLE 13

Dry Weight Results of Control and Transgenic (Over-expressing

osa-miRf11996-akr) Arabidopsis Plants.

Number of
Dry Weight %

Treatment
Strain
Dry weight +− SD
Plants
of Control

No
Control
483.353 +− 113.070
17

treatment
11996-2
305.909 +− 88.033
22
63.29%

11996-3
231.500 +− 72.002
26
47.90%

11996-5
179.194 +− 71.185
31
37.07%

11996-7
285.158 +− 101.236
19
59.00%

Drought
Control
225.478 +− 99.687
23

11996-2
170.000 +− 60.393
21
75.40%

11996-3
157.269 +− 63.415
26
69.75%

11996-5
186.353 +− 92.723
20
82.65%

11996-7
179.818 +− 68.019
22
79.75%

Salinity
Control
210.895 +− 76.991
19

11996-2
77.143 +− 41.214
21
36.58%

11996-3
109.130 +− 60.144
23
51.75%

11996-5
103.480 +− 44.930
25
49.07%

11996-7
116.789 +− 64.514
19
55.38%

Example 8
Selection of Transgenic Arabidopsis Plants Expressing Abiotic Stress Genes According to Expression Level

Arabidopsis seeds are sown and Basta is sprayed for the first time on 1-2 weeks old seedlings, at least twice every few days. Only resistant plants, which are heterozygous for the transgene, survive. PCR on the genomic gene sequence is performed on the surviving seedlings using primers pORE-F2 (fwd, 5′-TTTAGCGATGAACTTCACTC-3′, SEQ ID NO: 11614) and a custom designed reverse primer based on each small RNA sequence.

Example 9
Evaluating Changes in Root Architecture of Transgenic Plants

Many key traits in modern agriculture can be explained by changes in the root architecture of the plant. Root size and depth have been shown to logically correlate with drought tolerance and fertilizer use efficiency, since deeper and more branched root systems provide better coverage of the soil and can access water and nutrients stored in deeper soil layers.

To test whether the transgenic plants produce a modified root structure, plants can be grown in agar plates placed vertically. A digital picture of the plates is taken every few days and the maximal length and total area covered by the plant roots are assessed. From every construct created, several independent transformation events are checked in replicates. To assess significant differences between root features, statistical test, such as a Student's t-test, is employed in order to identify enhanced root features and to provide a statistical value to the findings.

Example 10
Abiotic Stress Tolerance Assessments of Control and Transgenic Plants

Transgenic plants expressing the polynucleotides of some embodiments of the invention exhibit tolerance to abiotic stress in the form of extreme deficiency in water, high salt concentrations, or heat shock and exhibit better overall survival and growth compared to control non-transgenic plants.

Quantitative parameters of tolerance measured include, but are not limited to, the average wet and dry weight, growth rate, leaf size, leaf coverage (overall leaf area), the weight of the seeds yielded, the average seed size and the number of seeds produced per plant. Under normal conditions (non-stress, optimal growth conditions), transgenic plants exhibit a phenotype equivalent or superior to that of the wild type plants. Following stress induction, transformed plants not exhibiting substantial physiological and/or morphological effects, or exhibiting higher biomass than wild-type plants, are identified as abiotic stress tolerant plants. Following are a number of screens aimed at identifying the transgenic plants which exhibit abiotic stress tolerance according to some embodiments of the invention.

Soil-Based Drought Tolerance Assay

Screens are performed with plants over-expressing the differential small RNAs detailed above. Briefly, seeds from control Arabidopsis plants, or other transgenic plants over-expressing the small RNA molecule of the invention are germinated and transferred to pots. Drought stress is obtained when irrigation is ceased and the two plant types (transgenic and control plants) are compared when most control plants develop severe wilting, at which point rehydration of the plants is initiated. Transgenic plants are ranked on two levels compared to controls: (1) tolerance to drought conditions, and (2) recovery (survival) following re-watering.

To illustrate and elaborate on the above drought tolerance assays of any given wild type plant compared to a corresponding transgenic plant (in which a drought-associated miRNA has been over-expressed), two different approaches are taken as follows:

Lethal drought stress—whereby wild type (used as a control) and transgenic plants (1-3 weeks old) are grown under prolonged extreme drought conditions (duration varies in accordance with plant species). Next, a recovery attempt is implemented during which plants are regularly irrigated and survival level is estimated in the two plant groups 1-2 days post irrigation initiation. While the control (wild type) plant is not expected to survive this extreme stress, the transgenic plant is expected to demonstrate some improved drought tolerance, usually within hours of re-hydration.

Non-lethal drought stress—whereby wild type (used as a control) and transgenic plants (1-3 weeks old) are grown under regular short-term cycles of drought and re-hydration steps, such that re-hydration is applied when general visible drought symptoms (e.g., evident decrease in turgor pressure of lower leaves) emerge in the experimental plants. This drought/irrigation alternating treatment continues until the flowering stage of the plants is reached, followed by an evaluation of dry matter weight. Both wild type and transgenic plants are expected to survive this non-lethal stress, however, measurable differences in drought tolerance are demonstrated by increased yield of the transgenic compared with the wild type plants.

Drought Tolerance Assay Using Sorbitol

Another assay designed to assess whether transgenic plants are more tolerant to drought or severe water deprivation compared to control plants, involves induction of an osmotic stress by the non-ionic osmolyte sorbitol. Control and transgenic plants are germinated and grown in plant-agar plates for 4 days, after which they are transferred to plates containing 500 mM sorbitol, to cause delayed growth. Following the stress treatment, control and transgenic plants are compared by measuring plant weight (wet and dry), yield, and growth rate measured as time to flowering.

Methods for Salinity Tolerance Assessment

Osmotic stress assays, such as chloride and mannitol assays, are aimed to determine whether an osmotic stress phenotype is sodium chloride-specific or a result of a general osmotic stress. Plants which are tolerant to osmotic stress may also exhibit tolerance to drought and/or freezing. For salt and osmotic stress germination experiments, the medium is supplemented with 50, 100, or 200 mM NaCl or 100 mM, 200 mM NaCl, 400 mM mannitol.

Methods for Heat Stress Tolerance Assessment

Heat stress tolerance is achieved by exposing the plants to temperatures above 34° C. for a certain period. Plant tolerance is examined after transferring the plants back to 22° C. for recovery and evaluation after 5 days relative to internal controls (non-transgenic plants) or plants not exposed to neither cold nor heat stress.

Methods for Cold Stress Tolerance Assessment

To analyze cold stress, mature (25 day old) plants are transferred to 4° C. chambers for 1 or 2 weeks, with constitutive light. Next, plants are moved back to the greenhouse for 2 weeks to recover. Following the recovery period, chilling damages such as growth retardation are determined based on measurements of plant weight (wet and dry) and growth rates (e.g. time to flowering, plant size, yield, etc) taken on control and transgenic plants.

Example 11
Testing Morphologic Parameters in Transgenic Plants

To analyze whether the transgenic Arabidopsis plants are more tolerant to abiotic stresses, plants are grown under optimal versus stress conditions, i.e either drought for five days without irrigation, or high salt conditions for ten days, or a one-hour heat shock. Plants are allowed to grow until seed production, followed by an analysis of their overall size, time to flowering, yield, and protein content of shoot and/or grain. Additional parameters checked can be the overall size of the plant, wet and dry weight, the weight of the seeds yielded, the average seed size and the number of seeds produced per plant. Transformed plants not exhibiting substantial physiological and/or morphological effects, or exhibiting higher measured parameters levels compared to wild-type plants, are identified as abiotic stress tolerant plants.

Example 12
Method for Generating Transgenic Plants with Enhanced or Reduced miRNA Regulation of Target Genes

Target prediction enables two contrasting strategies; an enhancement (positive) or a reduction (negative) of small RNA regulation. Both these strategies have been used in plants and have resulted in significant phenotype alterations. For complete in-vivo assessment of the phenotypic effects of the differential small RNAs of this invention, the inventors plan to implement both over-expression and down-regulation methods on the small RNA molecules found to associate with abiotic stress tolerance as listed in Tables 1-6. In the case of small RNAs that were up-regulated under abiotic stress conditions, an enhancement in abiotic stress tolerance can theoretically be achieved by maintaining their directionality, i.e. over-expressing them. Conversely, in the case of small RNAs that were down-regulated under abiotic stress conditions, enhancement in tolerance can be achieved by reducing their regulation. Regulation reduction of small RNA target genes can be accomplished in one of two approaches:

Expressing a miRNA-Resistant Target

In this method, silent mutations are introduced in the miRNA binding site of the target gene so that the DNA and resulting RNA sequences are changed to prevent miRNA binding, but the amino acid sequence of the protein is unchanged.

For design of miRNA-resistant target sequences for the small RNA molecules of the invention, optimization of the nucleic acid sequence in accordance with the preferred codon usage for a particular plant species is required. Tables such as those provided on-line at the Codon Usage Database through the NCBI (National Center for Biotechnology Information) webpage (Hypertext Transfer Protocol://World Wide Web (dot) ncbi (dot) nlm (dot) nih (dot) gov/Taxonomy/Utils/wprintgc (dot) cgi) were used. The Genbank database contains codon usage tables for a number of different species, with its Table 11 (The Bacterial, Archaeal and Plant Plastid Code) being the most relevant for plant species of this invention. Mir-resistant target examples for unregulated and downregulated miRs of the invention are presented in Tables 14-15 below.

TABLE 14

miRNA-Resistant Target Examples for Selected upregulated miRNAs

of the Invention.

NCBI
mutated

Mir
nucleotide

Mir

Binding
sequence/SEQ

name
Homolog NCBI Accession
Site
ID NO:

aqc-
XP_003543825

0

miR159

958-978
11091

958-978
11092

XP_003541563

0

1111-1131
11093

1111-1131
11094

XP_003556814

0

952-972
11095

952-972
11096

XP_003526354

0

928-948
11097

928-948
11098

XP_003523913

0

931-951
11099

931-951
11100

XP_003545791

0

934-954
11101

934-954
11102

ath-
XP_003542140

0

miR159b

404-424
11103

404-424
11104

ath-
XP_003519140

0

miR159c

143-163
11105

143-163
11106

XP_003531162

0

2030-2050
11107

2030-2050
11108

XP_003524148

0

1188-1208
11109

1188-1208
11110

XP_003547199

0

1263-1283
11111

1263-1283
11112

XP_003541668

0

1329-1349
11113

1329-1349
11114

ath-
XP_003525932

0

miRf10240-

akr

357-376
11115

357-376
11116

XP_003523287

0

864-883
11117

864-883
11118

XP_003547951

0

1451-1470
11119

1451-1470
11120

XP_003629354

0

1224-1243
11121

1224-1243
11122

ath-
XP_003543893

0

miRf10368-

akr

584-603
11123

584-603
11124

XP_003539013

0

599-618
11125

599-618
11126

XP_003556840

0

727-746
11127

727-746
11128

XP_003538207

0

1733-1752
11129

1733-1752
11130

ath-
XP_003520499

0

miRf10763-

akr

245-264
11131

245-264
11132

XP_003519685

0

240-259
11133

240-259
11134

ACU17625

0

176-195
11135

176-195
11136

XP_003527981

0

558-577
11137

558-577
11138

XP_003547100

0

1686-1705
11139

1686-1705
11140

XP_003524815

0

524-543
11141

524-543
11142

csi-
XP_003547789

0

miR3948

31-54
11143

31-54
11144

XP_003527776

0

46-69
11145

46-69
11146

XP_003550061

0

178-201
11147

178-201
11148

XP_003525811

0

178-201
11149

178-201
11150

XP_003539180

0

283-306
11151

283-306
11152

BAD18437

0

278-301
11153

278-301
11154

ghr-
XP_003529456

0

miR2950

1017-1037
11155

1017-1037
11156

XP_003554852

0

380-400
11157

380-400
11158

gma-
XP_003520455

0

miR156g

737-756
11159

737-756
11160

XP_003553428

0

734-753
11161

734-753
11162

XP_003520534

0

734-753
11163

734-753
11164

XP_003553944

0

1089-1108
11165

1089-1108
11166

XP_003551188

0

1046-1065
11167

1046-1065
11168

XP_003532399

0

941-960
11169

941-960
11170

XP_003549130

0

1034-1053
11171

1034-1053
11172

XP_003550514

0

706-725
11173

706-725
11174

XP_003525415

0

1214-1233
11175

1214-1233
11176

XP_003538544

0

1269-1288
11177

1269-1288
11178

XP_003525436

0

1333-1352
11179

1333-1352
11180

XP_003550708

0

1184-1203
11181

1184-1203
11182

XP_003520128

0

1007-1026
11183

1007-1026
11184

XP_003523155

0

959-978
11185

959-978
11186

XP_003551421

0

758-777
11187

758-777
11188

XP_003522278

0

1262-1281
11189

1262-1281
11190

gma-
Redundant target XP_003549130

0

miR157c

gma-
Redundant targets: XP_003542140, XP_003543825,

miR159a-
XP_003526354, XP_003541563, XP_003556814, XP_003545791,

3p
XP_003523913.

iba-
Redundant targets: XP_003520455, XP_003553428,

miR157
XP_003520534, XP_003553944, XP_003551188, XP_003532399,

XP_003549130, XP_003525415, XP_003538544, XP_003525436,

XP_003520128, XP_003551421, XP_003523155, XP_003550708,

XP_003522278

mdm-
XP_003528897

miR482a-

5p

940-960
11191

940-960
11192

940-960
11193

940-960
11194

osa-
Redundant targets: XP_003543825, XP_003531162,

miR159e
XP_003526354, XP_003524148, XP_003547199, XP_003541563,

XP_003556814, XP_003541668, XP_003523913, XP_003545791.

osa-
Redundant targets: XP_003543825, XP_003526354,

miR159f
XP_003541563, XP_003556814, XP_003523913, XP_003545791.

osa-
XP_003555849

miR1850.1

147-167
11195

147-167
11196

XP_003534041

0

29-49
11197

29-49
11198

XP_003548988

0

451-471
11199

451-471
11200

osa-
XP_003521247

0

miR1858a

287-307
11201

287-307
11202

NP_001235053

0

281-301
11203

281-301
11204

XP_003530234

0

368-388
11205

368-388
11206

XP_003551508

0

377-397
11207

377-397
11208

XP_003528545

0

131-151
11209

131-151
11210

XP_003547641

0

155-175
11211

155-175
11212

XP_003543554

0

185-205
11213

185-205
11214

XP_003556667

0

131-151
11215

131-151
11216

osa-
XP_003546711

0

miRf11829-

akr

346-366
11217

346-366
11218

ACI23460

0

334-354
11219

334-354
11220

XP_003541398

0

365-385
11221

365-385
11222

psi-
Redundant targets: XP_003543825, XP_003526354,

miR159
XP_003541563, XP_003556814, XP_003545791, XP_003523913.

pta-
Redundant targets: XP_003549130, XP_003553428,

miR156a
XP_003553944, XP_003525436, XP_003520455, XP_003520128,

XP_003550708, XP_003523155, XP_003551421, XP_003522278.

pta-
XP_003551276

miR156b

202-221
11223

202-221
11224

202-221
11225

202-221
11226

ptc-
XP_003527653

miRf10132-

akr

128-150
11227

128-150
11228

128-150
11229

ptc-
XP_003549610

0

miRf10226-

akr

123-145
11230

123-145
11231

XP_003525906

0

174-196
11232

174-196
11233

XP_003547131

0

2282-2304
11234

2282-2304
11235

XP_003542817

0

240-262
11236

240-262
11237

ptc-
Redundant targets: XP_003543825, XP_003526354,

miRf10271-
XP_003541563, XP_003556814, XP_003523913, XP_003545791.

akr

ptc-
XP_003520774

0

miRf10734-

akr

1439-1459
11238

1439-1459
11239

XP_003538849

0

1187-1207
11240

1187-1207
11241

ptc-
XP_003546504

0

miRf10985-

akr

1472-1491
11242

1472-1491
11243

XP_003545057

0

3399-3418
11244

3399-3418
11245

ptc-
XP_003550774

0

miRf11315-

akr

311-330
11246

311-330
11247

XP_003518840

0

255-274
11248

255-274
11249

ptc-
XP_003612685

0

miRf11757-

akr

388-410
11250

388-410
11251

388-410
11252

388-410
11253

ath-
Redundant targets: XP_003525415, XP_003553428,

miR157a
XP_003538544, XP_003553944, XP_003551188, XP_003525436,

XP_003520455, XP_003523155, XP_003551421, XP_003549130,

XP_003522278.

sbi-
Redundant targets: XP_003543825, XP_003526354,

miR159a
XP_003541563, XP_003556814.

smo-
XP_003528960

miR156b

462-482
11254

462-482
11255

462-482
11256

462-482
11257

TABLE 15

miRNA-Resistant Target Examples for Selected down-regulated

miRNAs of the Invention.

NCBI Mir
Mutated Nucleotide

Homolog NCBI
Binding
Sequence/

Mir name
Accession
Site
SEQ ID NO:

bdi-miR2508
XP_003530212

689-710
11258

689-710
11259

XP_003530213

0

794-815
11260

794-815
11261

XP_003551482

0

689-710
11262

689-710
11263

XP_003548937

0

1148-1169
11264

1148-1169
11265

XP_003551299

0

733-754
11266

733-754
11267

XP_003520176

0

2159-2180
11268

2159-2180
11269

XP_003544873

0

656-677
11270

656-677
11271

XP_003552227

0

665-686
11272

665-686
11273

XP_003539077

0

713-734
11274

713-734
11275

XP_003552179

0

701-722
11276

701-722
11277

NP_001236616

0

698-719
11278

698-719
11279

XP_003540719

0

707-728
11280

707-728
11281

XP_003522150

0

662-683
11282

662-683
11283

bra-miR160a-3p
XP_003530952

0

1283-1303
11284

1283-1303
11285

gma-miR2119
XP_003542005

0

212-232
11286

212-232
11287

XP_003521584

0

421-441
11288

421-441
11289

XP_003524240

0

1982-2002
11290

1982-2002
11291

XP_003545664

0

96-116
11292

96-116
11293

XP_003532800

0

1982-2002
11294

1982-2002
11295

XP_003547559

0

2084-2104
11296

2084-2104
11297

gso-miR482a
NP_001237600

0

1153-1173
11298

1153-1173
11299

XP_003533606

0

523-543
11300

523-543
11301

XP_003518623

0

444-464
11302

444-464
11303

AAF44087

0

Jan-21
11304

Jan-21
11305

osa-miR162a
XP_003528812

612-632
11306

612-632
11307

612-632
11308

612-632
11309

osa-miR1846e
XP_003531668

0

471-490
11310

471-490
11311

XP_003529761

0

363-382
11312

363-382
11313

XP_003530142

0

366-385
11314

366-385
11315

ppt-miR166m
XP_003553029

0

530-550
11316

530-550
11317

XP_003597690

0

875-895
11318

875-895
11319

XP_002285176

0

562-582
11320

562-582
11321

XP_003530109

0

906-926
11322

906-926
11323

XP_003524993

0

1030-1050
11324

1030-1050
11325

XP_003522716

0

825-845
11326

825-845
11327

XP_003530112

0

606-626
11328

606-626
11329

XP_003532788

0

577-597
11330

577-597
11331

XP_003537529

0

515-535
11332

515-535
11333

XP_003531653

0

777-797
11334

777-797
11335

XP_003539764

0

1227-1247
11336

1227-1247
11337

XP_003539765

0

1227-1247
11338

1227-1247
11339

ptc-miRf10007-akr
XP_003550796

1378-1398
11340

1378-1398
11341

1378-1398
11342

1378-1398
11343

ptc-miRf10976-akr
XP_003533044

0

318-337
11344

318-337
11345

XP_003528486

0

267-286
11346

267-286
11347

XP_003548151

0

885-904
11348

885-904
11349

NP_001238468

0

245-264
11350

245-264
11351

ptc-miRf11396-akr
XP_003520116

764-785
11352

764-785
11353

764-785
11354

764-785
11355

ptc-miRf11669-akr
XP_003554103

208-227
11356

208-227
11357

208-227
11358

208-227
11359

Expressing a Target-Mimic Sequence

Plant miRNAs usually lead to cleavage of their targeted gene, with this cleavage typically occurring between bases 10 and 11 of the miRNA. This position is therefore especially sensitive to mismatches between the miRNA and the target. It was found that expressing a DNA sequence that could potentially be targeted by a miRNA, but contains three extra nucleotides (ATC), and thus creating a bulge in a key position (between the two nucleotides that are predicted to hybridize with bases 10-11 of the miRNA), can inhibit the regulation of that miRNA on its native targets (Franco-Zorilla et al., 2007, Nat Genet 39(8):1033-1037).

This type of sequence is referred to as a “target-mimic”. Inhibition of the miRNA regulation is presumed to occur through physically capturing the miRNA by the target-mimic sequence and titering-out the miRNA, thereby reducing its abundance. This method was used to reduce the amount and, consequentially, the regulation of miRNA 399 in Arabidopsis. Target mimic examples for upregulated and downregulated miRs of the invention are presented in Tables 16-17 below.

TABLE 16

Target Mimic Examples for Selected upregulated miRNAs of the

Invention.

Mimic Reverse
Full Target Mimic

Complement Mir/SEQ ID
Nucleotide Sequence/

Mir Name
NO:
SEQ ID NO:

ahy-miR3514-
11360
11437

5p

aly-miR831-
11361
11438

5p

aqc-miR159
11362
11439

ath-miR157a
11363
11440

ath-miR159b
11364
11441

ath-miR159c
11365
11442

ath-
11366
11443

miRf10068-

akr

ath-
11367
11444

miRf10148-

akr

ath-
11368
11445

miRf10209-

akr

ath-
11369
11446

miRf10240-

akr

ath-
11370
11447

miRf10368-

akr

ath-
11371
11448

miRf10451-

akr

ath-
11372
11449

miRf10633-

akr

ath-
11373
11450

miRf10687-

akr

ath-
11374
11451

miRf10701-

akr

ath-
11375
11452

miRf10702-

akr

ath-
11376
11453

miRf10751-

akr

ath-
11377
11454

miRf10763-

akr

ath-
11378
11455

miRf10924-

akr

ath-
11379
11456

miRf11021-

akr

ath-
11380
11457

miRf11037-

akr

ath-
11381
11458

miRf11042-

akr

ath-
11382
11459

miRf11045-

akr

csi-miR3946
11383
11460

csi-miR3948
11384
11461

far-miR1134
11385
11462

ghr-miR2950
11386
11463

gma-miR156g
11387
11464

gma-miR157c
11388
11465

gma-
11389
11466

miR159a-3p

iba-miR157
11390
11467

mdm-
11391
11468

miR482a-5p

mtr-miR2119
11392
11469

osa-miR159e
11393
11470

osa-miR159f
11394
11471

osa-
11395
11472

miR1850.1

osa-miR1858a
11396
11473

osa-miR1869
11397
11474

osa-miR1874-
11398
11475

3p

osa-miR1879
11399
11476

osa-miR1881
11400
11477

osa-miR2055
11401
11478

osa-
11402
11479

miRf10105-

akr

osa-
11403
11480

miRf10362-

akr

osa-
11404
11481

miRf10839-

akr

osa-
11405
11482

miRf11013-

akr

osa-
11406
11483

miRf11341-

akr

osa-
11407
11484

miRf11352-

akr

osa-
11408
11485

miRf11355-

akr

osa-
11409
11486

miRf11595-

akr

osa-
11410
11487

miRf11649-

akr

osa-
11411
11488

miRf11829-

akr

pab-miR3711
11412
11489

ppt-miR1220a
11413
11490

ppt-miR895
11414
11491

psi-miR159
11415
11492

pta-miR156a
11416
11493

pta-miR156b
11417
11494

ptc-
11418
11495

miRf10132-

akr

ptc-
11419
11496

miRf10148-

akr

ptc-
11420
11497

miRf10226-

akr

ptc-
11421
11498

miRf10271-

akr

ptc-
11422
11499

miRf10300-

akr

ptc-
11423
11500

miRf10522-

akr

ptc-
11424
11501

miRf10619-

akr

ptc-
11425
11502

miRf10734-

akr

ptc-
11426
11503

miRf10985-

akr

ptc-
11427
11504

miRf11315-

akr

ptc-
11428
11505

miRf11757-

akr

ptc-
11429
11506

miRf11844-

akr

ptc-
11430
11507

miRf11847-

akr

ptc-
11431
11508

miRf11855-

akr

sbi-miR159a
11432
11509

smo-
11433
11510

miR1103-3p

smo-miR156b
11434
11511

tae-miR2003
11435
11512

zma-miR482-
11436
11513

5p

TABLE 17

Target Mimic Examples for Selected downregulated miRNAs of the

Invention.

Full

Target Mimic

Nucleotide

Mimic Reverse
Sequence/SEQ ID

Mir Name
Complement Mir/SEQ ID NO:
NO:

aly-miR160c-3p
11514
11564

aly-miR396a-3p
11515
11565

aly-miR396b-3p
11516
11566

ath-miRf10197-akr
11517
11567

ath-miRf10239-akr
11518
11568

ath-miRf10279-akr
11519
11569

bdi-miR2508
11520
11570

bna-miR2111b-5p
11521
11571

bra-miR160a-3p
11522
11572

csi-miR162-5p
11523
11573

ctr-miR171
11524
11574

gma-miR1507a
11525
11575

gma-miR1524
11526
11576

gma-miR159d
11527
11577

gma-miR2119
11528
11578

gma-miR396d
11529
11579

gma-miR4371b
11530
11580

gma-miR4376-5p
11531
11581

gma-miR4412-3p
11532
11582

gma-miR4416a
11533
11583

gma-miR482a-3p
11534
11584

gma-miR482b-5p
11535
11585

gso-miR169g*
11536
11586

gso-miR482a
11537
11587

osa-miR162a
11538
11588

osa-miR1846e
11539
11589

osa-miR2104
11540
11590

osa-miRf10151-akr
11541
11591

osa-miRf10849-akr
11542
11592

osa-miRf11415-akr
11543
11593

osa-miRf11996-akr
11544
11594

ppt-miR166m
11545
11595

ppt-miR533b-5p
11546
11596

pta-miR166c
11547
11597

ptc-miR166p
11548
11598

ptc-miRf10007-akr
11549
11599

ptc-miRf10976-akr
11550
11600

ptc-miRf11018-akr
11551
11601

ptc-miRf11079-akr
11552
11602

ptc-miRf11324-akr
11553
11603

ptc-miRf11396-akr
11554
11604

ptc-miRf11669-akr
11555
11605

ptc-miRf11953-akr
11556
11606

ptc-miRf12069-akr
11557
11607

ptc-miRf12389-akr
11558
11608

vvi-miR2111-5p
11559
11609

vvi-miR394b
11560
11610

zma-miR167u
11561
11611

zma-miR396b-3p
11562
11612

zma-miR398a-5p
11563
11613

TABLE 18

Abbreviations of plant species

Common Name
Organism Name
Abbreviation

Peanut
Arachis hypogaea
ahy

Arabidopsis lyrata

Arabidopsis lyrata

aly

Rocky Mountain Columbine

Aquilegia coerulea

aqc

Tausch's goatgrass

Aegilops taushii

ata

Arabidopsis thaliana

Arabidopsis thaliana

ath

Grass

Brachypodium distachyon

bdi

Brassica napus canola (“liftit”)

Brassica napus

bna

Brassica oleracea wild cabbage

Brassica oleracea

bol

Brassica rapa yellow mustard

Brassica rapa

bra

Clementine

Citrus clementine

ccl

Orange

Citrus sinensis

csi

Trifoliate orange

Citrus trifoliata

ctr

Glycine max

Glycine max

gma

Wild soybean

Glycine soja

gso

Barley

Hordeum vulgare

hvu

Lotus japonicus

Lotus japonicus

lja

Medicago truncatula - Barrel Clover (“tiltan”)

Medicago truncatula

mtr

Oryza sativa

Oryza sativa

osa

European spruce

Picea abies

pab

Physcomitrella patens (moss)

Physcomitrella patens

ppt

Pinus taeda - Loblolly Pine

Pinus taeda

pta

Populus trichocarpa - black cotton wood

Populus trichocarpa

ptc

Castor bean (“kikayon”)

Ricinus communis

rco

Sorghum bicolor Dura

Sorghum bicolor

sbi

tomato microtom

Solanum lycopersicum

sly

Selaginella moellendorffii

Selaginella moellendorffii

smo

Sugarcane

Saccharum officinarum

sof

Sugarcane

Saccharum spp
ssp

Triticum aestivum

Triticum aestivum

tae

cacao tree and cocoa tree

Theobroma cacao

tcc

Vitis vinifera Grapes

Vitis vinifera

vvi

corn

Zea mays

zma

Although the invention has been described in conjunction with specific embodiments thereof, it is evident that many alternatives, modifications and variations will be apparent to those skilled in the art. Accordingly, it is intended to embrace all such alternatives, modifications and variations that fall within the spirit and broad scope of the appended claims.

All publications, patents and patent applications mentioned in this specification are herein incorporated in their entirety by reference into the specification, to the same extent as if each individual publication, patent or patent application was specifically and individually indicated to be incorporated herein by reference. In addition, citation or identification of any reference in this application shall not be construed as an admission that such reference is available as prior art to the present invention. To the extent that section headings are used, they should not be construed as necessarily limiting.

Number	Name	Date	Kind
3791932	Schuurs et al.	Feb 1974	A
3839153	Schuurs et al.	Oct 1974	A
3850578	McConnell	Nov 1974	A
3850752	Schuurs et al.	Nov 1974	A
3853987	Dreyer	Dec 1974	A
3867517	Ling	Feb 1975	A
3879262	Schuurs et al.	Apr 1975	A
3901654	Gross	Aug 1975	A
3935074	Rubenstein et al.	Jan 1976	A
3984533	Uzgiris	Oct 1976	A
3996345	Ullman et al.	Dec 1976	A
4034074	Miles	Jul 1977	A
4098876	Piasio et al.	Jul 1978	A
4666828	Gusella	May 1987	A
4683202	Mullis	Jul 1987	A
4801531	Frossard	Jan 1989	A
4855237	Morinaga et al.	Aug 1989	A
4879219	Wands et al.	Nov 1989	A
4945050	Sanford	Jul 1990	A
5011771	Bellet et al.	Apr 1991	A
5035323	Daniels et al.	Jul 1991	A
5192659	Simons	Mar 1993	A
5268463	Jefferson	Dec 1993	A
5272057	Smulson et al.	Dec 1993	A
5281521	Trojanowski et al.	Jan 1994	A
5283185	Epand et al.	Feb 1994	A
5316931	Donson et al.	May 1994	A
5399680	Zhu et al.	Mar 1995	A
5464765	Coffee et al.	Nov 1995	A
5466785	de Framond	Nov 1995	A
5569597	Grimsley et al.	Oct 1996	A
5604121	Hilder et al.	Feb 1997	A
5608142	Barton et al.	Mar 1997	A
5608144	Baden et al.	Mar 1997	A
5608149	Barry et al.	Mar 1997	A
5659026	Baszczynski et al.	Aug 1997	A
5693507	Daniell et al.	Dec 1997	A
5759829	Shewmaker et al.	Jun 1998	A
5952657	Alexander et al.	Sep 1999	A
5987071	Iwamatsu et al.	Nov 1999	A
6656805	Kamath et al.	Dec 2003	B2
20020058815	Liu et al.	May 2002	A1
20030175965	Lowe et al.	Sep 2003	A1
20030180955	Ozasa et al.	Sep 2003	A1
20070089192	Huang et al.	Apr 2007	A1
20080311659	Huynh et al.	Dec 2008	A1
20110099667	Aukerman et al.	Apr 2011	A1

Number	Date	Country
WO 8706261	Oct 1987	WO
WO 9307278	Apr 1993	WO
WO 9953050	Oct 1999	WO
WO 9959029	Nov 1999	WO
WO 9961631	Dec 1999	WO
WO 0059035	Oct 2000	WO
WO 0200905	Jan 2002	WO
WO 2004081173	Sep 2004	WO
WO 2011132127	Oct 2011	WO
WO 2011132127	Oct 2011	WO
WO 2013118120	Aug 2013	WO

Isolated polynucleotides expressing or modulating microRNAs or targets of same, transgenic plants comprising same and uses thereof

Information

Patent Number

Date Filed

Date Issued

Inventors

Original Assignees

Examiners

Agents

CPC

Field of Search

US

International Classifications

Term Extension

Abstract

Description

Claims

RELATED APPLICATIONS

PCT Information

US Referenced Citations (47)

Foreign Referenced Citations (11)

Non-Patent Literature Citations (115)

Related Publications (1)

Provisional Applications (1)

Entry
Emery et al. (Current Biology 13:1768-1774, 2003).
Cheng et al. (Plant Mol. Biol. Rep. 28:41-48, 2010).
International Preliminary Report on Patentability dated Aug. 21, 2014 From the International Bureau of WIPO Re. Application No. PCT/IL2013/050112.
International Search Report and the Written Opinion dated Aug. 2, 2013 From the International Searching Authority Re. Application No. PCT/IL2013/050112.
International Search Report and the Written Opinion dated Oct. 21, 2013 From the International Searching Authority Re. Application No. PCT/IL2013/050112.
Invitation to Pay Additional Fees dated May 30, 2013 From the International Searching Authority Re. Application No. PCT/IL2013/050112.
Lindow et al. “Intragenomic Matching Reveals a Huge Potential for MiRNA-Mediated Regulation in Plants”, PLoS Computational Biology, 3(11/e238): 2379-2390, Nov. 2007. Abstract.
Lindow et al. “Oeyza Sativa. Osa-MRIfI 1996-Akr. Stem-Loop Information. Stem Loop Structure Computed Using [Vienna RNA Package]”, The miRNA Database [Online], 3 p., 2007.
Zhao et al. “Deep Sequencing Identifies Novel and Conserved MicroRNAs in Peanuts (Arachis hypogaea L.)”, BMC Plant Biology, 10(3): 1-12, 2010. Abstract, p. 4-5, Table 1, 2.
Bartel, “MicroRNAs: Genomics, Biogenesis, Mechanism, and Function,” Cell, 116:281-297 (2004).
Boutros et al., “Genome-Wide RNAi Analysis of Growth and Viability in Drosophila Cells,” Science, 303:832-835 (2004).
Dietzl et al., “A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila,” Nature, 448:151-156 (2007).
Kuromori et al., “A trial of phenome analysis using 4000 Ds-insertional mutants in gene-coding regions of Arabidopsis,” Plant Journal, 47:640-651 (2006).
Lee et al., “A systematic RNAi screen identifies a critical role for mitochondria in C. elegans longevity,” Nature Genetics, 33:40-48 (2002).
Ni et al., “Overexpression of gma-MIR394a confers tolerance to drought in transgenic Arabidopsis thaliana,” Biochem Biophys Res Commun, 427:330-335 (2012).
Schwab et al., “Specific Effects of MicroRNAs on the Plant Transcriptome,” Developmental Cell, 8:517-527 (2005).
Covarrubias et al., “Post-transcriptional gene regulation of salinity and drought responses by plant microRNAs,” Plant, Cell & Environment, 33:481-489 (2010).
Ochman et al., “Genetic Applications of an Inverse Polymerase Chain Reaction,” Genetics, 120:621-623 (1988).
Rhoades et al., “Prediction of Plant MicroRNA Targets,” Cell, 110:513-520 (2002).
Schmutz et al., “Genome sequence of the palaeopolyploid soybean,” Nature, 463:178-183 (2010).
Zhang, “miRU: an automated plant miRNA target prediction server,” Nucleic Acids Research, 33:W701-W704 (2005).
Albani et al., “The Wheat Transcriptional Activator Spa: A Seed-Specific bZIP Protein That Recgnizes the GCN4-Like Motif in the Bifactorial Endosperm Box of Prolamin Genes,” The Plant Cell, 9(2):171-184 (1997).
Altenbach et al., “Accumulation of a Brazil nut albumin in seeds of transgenic canola results in enhanced levels of seed protein methionine,” Plant Molecular Biology, 18:235-245 (1992).
An et al., “Strong, constitutive expression of the Arabidopsis ACT2/ACT8 actin subclass in vegetative tissues,” The Plant Journal, 10(1):107-121 (1996).
Anderson et al., “Nucleotide sequences of the two high-molecular-weight glutenin genes from the D-genome of a hexaploid bread wheat, Triticum aestivum L. cv Cheyenne,” Nucleic Acids Research, 17(1):461-461 (1989).
Angell et al ., “Consistent gene silencing in transgenic plants expressing a replicating potato virus X RNA,” EMBO J., 16(12):3675-3684 (1997).
Angell et al., “Potato virus X amplicon-mediated silencing of nuclear genes,” The Plant Journal, 20(3):357-362 (1999).
Aufsatz et al., “RNA-directed DNA methylation in Arabidopsis,” PNAS, 99(4):1699-16506 (2002).
Baszczynski et al., “Isolation and nucleotide sequence of a genomic clone encoding a new Brassica napus napin gene,” Plant Molecular Biology, 14:633-635 (1990).
Broin et al., “The Plastidic 2-Cysteine Peroxiredoxin Is a Target for a Thioredoxin Involved in the Protection of the Photosynthetic Apparatus against Oxidative Damage,” The Plant Cell, 14:1417-1432 (2002).
Buchholz et al., “Cyclophilins are encoded by a small gene family in rice,” Plant Molecular Biology, 25:837-843 (1994).
Chen et al., “Real-time quantification of microRNAs by stem-loop RT-PCR,” Nucleic Acids Research, 33(20):e179 (2005).
Cho et al., “Inheritance of tissue-specific expression of barley hordein promoter-uidA fusions in transgenic barley plants,” Theor Appl Genet, 98:1253-1262 (1999).
Christensen et al., “Maize polyubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation,” Plant Molecular Biology, 18:675-689 (1992).
Chuang et al., “Specific and heritable genetic interference by double-stranded RNA in Arabidopsis,” PNAS, 97(9):4985-4990 (2000).
Clough et al., “Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana,” The Plant Journal, 16(6):735-743 (1998).
Colot et al., “Molecular Characterization of an active wheat LMW glutenin gene and its relation to other wheat and barley prolamin genes,” Mol Gen Genet, 216:81-90 (1989).
Cummins et al., “cDNA sequence of a sunflower oleosin and transcript tissue specificity,” Plant Molecular Biology, 19:873-876 (1992).
Dawson et al., “A Tobacco Mosaic Virus-Hybrid Expresses and Loses an Added Gene,” Virology, 172:285-292 (1989).
De Pater et al., “The promoter of the rice gene GOS2 is active in various different monocot tissues and binds rice nuclear factor ASF-1,” The Plant Journal, 2(6):837-844 (1992).