ISOLATED POLYNUCLEOTIDES, POLYPEPTIDES AND METHODS OF USING SAME FOR INCREASING ABIOTIC STRESS TOLERANCE, BIOMASS AND YIELD OF PLANTS

FIELD AND BACKGROUND OF THE INVENTION

The present invention, in some embodiments thereof, relates to isolated polypeptides and polynucleotides, nucleic acid constructs comprising same, transgenic plants expressing same and methods of using same for increasing abiotic stress tolerance (ABST), water use efficiency (WUE), yield (e.g., grain quantity and/or quality), fiber yield, fiber quality, biomass, oil content, growth rate, vigor, nitrogen use efficiency (NUE) and/or fertilizer use efficiency (FUE) of a plant.

Abiotic stress (ABS; also referred to as “environmental stress”) conditions such as salinity, drought, flood, suboptimal temperature and toxic chemical pollution, cause substantial damage to agricultural plants. Most plants have evolved strategies to protect themselves against these conditions. However, if the severity and duration of the stress conditions are too great, the effects on plant development, growth and yield of most crop plants are profound. Furthermore, most of the crop plants are highly susceptible to abiotic stress and thus necessitate optimal growth conditions for commercial crop yields. Continuous exposure to stress causes major alterations in the plant metabolism which ultimately leads to cell death and consequently yield losses.

Drought is a gradual phenomenon, which involves periods of abnormally dry weather that persists long enough to produce serious hydrologic imbalances such as crop damage, water supply shortage and increased susceptibility to various diseases. In severe cases, drought can last many years and results in devastating effects on agriculture and water supplies. Furthermore, drought is associated with increase susceptibility to various diseases.

For most crop plants, the land regions of the world are too arid. In addition, overuse of available water results in increased loss of agriculturally-usable land (desertification), and increase of salt accumulation in soils adds to the loss of available water in soils.

Salinity, high salt levels, affects one in five hectares of irrigated land. None of the top five food crops, i.e., wheat, corn, rice, potatoes, and soybean, can tolerate excessive salt. Detrimental effects of salt on plants result from both water deficit, which leads to osmotic stress (similar to drought stress), and the effect of excess sodium ions on critical biochemical processes. As with freezing and drought, high salt causes water deficit; and the presence of high salt makes it difficult for plant roots to extract water from their environment. Soil salinity is thus one of the more important variables that determine whether a plant may thrive. In many parts of the world, sizable land areas are uncultivable due to naturally high soil salinity. Thus, salination of soils that are used for agricultural production is a significant and increasing problem in regions that rely heavily on agriculture, and is worsen by over-utilization, over-fertilization and water shortage, typically caused by climatic change and the demands of increasing population. Salt tolerance is of particular importance early in a plant's lifecycle, since evaporation from the soil surface causes upward water movement, and salt accumulates in the upper soil layer where the seeds are placed. On the other hand, germination normally takes place at a salt concentration which is higher than the mean salt level in the whole soil profile.

Salt and drought stress signal transduction consist of ionic and osmotic homeostasis signaling pathways. The ionic aspect of salt stress is signaled via the SOS pathway where a calcium-responsive SOS3-SOS2 protein kinase complex controls the expression and activity of ion transporters such as SOS1. The osmotic component of salt stress involves complex plant reactions that overlap with drought and/or cold stress responses.

Suboptimal temperatures affect plant growth and development through the whole plant life cycle. Thus, low temperatures reduce germination rate and high temperatures result in leaf necrosis. In addition, mature plants that are exposed to excess of heat may experience heat shock, which may arise in various organs, including leaves and particularly fruit, when transpiration is insufficient to overcome heat stress. Heat also damages cellular structures, including organelles and cytoskeleton, and impairs membrane function. Heat shock may produce a decrease in overall protein synthesis, accompanied by expression of heat shock proteins, e.g., chaperones, which are involved in refolding proteins denatured by heat. High-temperature damage to pollen almost always occurs in conjunction with drought stress, and rarely occurs under well-watered conditions. Combined stress can alter plant metabolism in novel ways. Excessive chilling conditions, e.g., low, but above freezing, temperatures affect crops of tropical origins, such as soybean, rice, maize, and cotton. Typical chilling damage includes wilting, necrosis, chlorosis or leakage of ions from cell membranes. The underlying mechanisms of chilling sensitivity are not completely understood yet, but probably involve the level of membrane saturation and other physiological deficiencies. Excessive light conditions, which occur under clear atmospheric conditions subsequent to cold late summer/autumn nights, can lead to photoinhibition of photosynthesis (disruption of photosynthesis). In addition, chilling may lead to yield losses and lower product quality through the delayed ripening of maize.

Common aspects of drought, cold and salt stress response [Reviewed in Xiong and Zhu (2002) Plant Cell Environ. 25: 131-139] include: (a) transient changes in the cytoplasmic calcium levels early in the signaling event; (b) signal transduction via mitogen-activated and/or calcium dependent protein kinases (CDPKs) and protein phosphatases; (c) increases in abscisic acid levels in response to stress triggering a subset of responses; (d) inositol phosphates as signal molecules (at least for a subset of the stress responsive transcriptional changes; (e) activation of phospholipases which in turn generates a diverse array of second messenger molecules, some of which might regulate the activity of stress responsive kinases; (f) induction of late embryogenesis abundant (LEA) type genes including the CRT/DRE responsive COR/RD genes; (g) increased levels of antioxidants and compatible osmolytes such as proline and soluble sugars; and (h) accumulation of reactive oxygen species such as superoxide, hydrogen peroxide, and hydroxyl radicals. Abscisic acid biosynthesis is regulated by osmotic stress at multiple steps. Both ABA-dependent and -independent osmotic stress signaling first modify constitutively expressed transcription factors, leading to the expression of early response transcriptional activators, which then activate downstream stress tolerance effector genes.

Several genes which increase tolerance to cold or salt stress can also improve drought stress protection, these include for example, the transcription factor AtCBF/DREB1, OsCDPK7 (Saijo et al. 2000, Plant J. 23: 319-327) or AVP1 (a vacuolar pyrophosphatase-proton pump, Gaxiola et al. 2001, Proc. Natl. Acad. Sci. USA 98: 11444-11449).

Studies have shown that plant adaptations to adverse environmental conditions are complex genetic traits with polygenic nature. Conventional means for crop and horticultural improvements utilize selective breeding techniques to identify plants having desirable characteristics. However, selective breeding is tedious, time consuming and has an unpredictable outcome. Furthermore, limited germplasm resources for yield improvement and incompatibility in crosses between distantly related plant species represent significant problems encountered in conventional breeding. Advances in genetic engineering have allowed mankind to modify the germplasm of plants by expression of genes-of-interest in plants. Such a technology has the capacity to generate crops or plants with improved economic, agronomic or horticultural traits.

Genetic engineering efforts, aimed at conferring abiotic stress tolerance to transgenic crops, have been described in various publications [Apse and Blumwald (Curr Opin Biotechnol. 13:146-150, 2002), Quesada et al. (Plant Physiol. 130:951-963, 2002), Holmström et al. (Nature 379: 683-684, 1996), Xu et al. (Plant Physiol 110: 249-257, 1996), Pilon-Smits and Ebskamp (Plant Physiol 107: 125-130, 1995) and Tarczynski et al. (Science 259: 508-510, 1993)].

Various patents and patent applications disclose genes and proteins which can be used for increasing tolerance of plants to abiotic stresses. These include for example, U.S. Pat. Nos. 5,296,462 and 5,356,816 (for increasing tolerance to cold stress); U.S. Pat. No. 6,670,528 (for increasing ABST); U.S. Pat. No. 6,720,477 (for increasing ABST); U.S. application Ser. Nos. 09/938,842 and 10/342,224 (for increasing ABST); U.S. application Ser. No. 10/231,035 (for increasing ABST); WO2004/104162 (for increasing ABST and biomass); WO2007/020638 (for increasing ABST, biomass, vigor and/or yield); WO2007/049275 (for increasing ABST, biomass, vigor and/or yield); WO2010/076756 (for increasing ABST, biomass and/or yield). WO2009/083958 (for increasing water use efficiency, fertilizer use efficiency, biotic/abiotic stress tolerance, yield and/or biomass); WO2010/020941 (for increasing nitrogen use efficiency, abiotic stress tolerance, yield and/or biomass); WO2009/141824 (for increasing plant utility); WO2010/049897 (for increasing plant yield).

Nutrient deficiencies cause adaptations of the root architecture, particularly notably for example is the root proliferation within nutrient rich patches to increase nutrient uptake. Nutrient deficiencies cause also the activation of plant metabolic pathways which maximize the absorption, assimilation and distribution processes such as by activating architectural changes. Engineering the expression of the triggered genes may cause the plant to exhibit the architectural changes and enhanced metabolism also under other conditions.

In addition, it is widely known that the plants usually respond to water deficiency by creating a deeper root system that allows access to moisture located in deeper soil layers. Triggering this effect will allow the plants to access nutrients and water located in deeper soil horizons particularly those readily dissolved in water like nitrates.

A common approach to promote plant growth has been, and continues to be, the use of natural as well as synthetic nutrients (fertilizers). Thus, fertilizers are the fuel behind the “green revolution”, directly responsible for the exceptional increase in crop yields during the last 40 years, and are considered the number one overhead expense in agriculture. For example, inorganic nitrogenous fertilizers such as ammonium nitrate, potassium nitrate, or urea, typically accounts for 40% of the costs associated with crops such as corn and wheat. Of the three macronutrients provided as main fertilizers [Nitrogen (N), Phosphate (P) and Potassium (K)], nitrogen is often the rate-limiting element in plant growth and all field crops have a fundamental dependence on inorganic nitrogenous fertilizer. Nitrogen is responsible for biosynthesis of amino and nucleic acids, prosthetic groups, plant hormones, plant chemical defenses, etc. and usually needs to be replenished every year, particularly for cereals, which comprise more than half of the cultivated areas worldwide. Thus, nitrogen is translocated to the shoot, where it is stored in the leaves and stalk during the rapid step of plant development and up until flowering. In corn for example, plants accumulate the bulk of their organic nitrogen during the period of grain germination, and until flowering. Once fertilization of the plant has occurred, grains begin to form and become the main sink of plant nitrogen. The stored nitrogen can be then redistributed from the leaves and stalk that served as storage compartments until grain formation.

Since fertilizer is rapidly depleted from most soil types, it must be supplied to growing crops two or three times during the growing season. In addition, the low nitrogen use efficiency (NUE) of the main crops (e.g., in the range of only 30-70%) negatively affects the input expenses for the farmer, due to the excess fertilizer applied.

Moreover, the over and inefficient use of fertilizers are major factors responsible for environmental problems such as eutrophication of groundwater, lakes, rivers and seas, nitrate pollution in drinking water which can cause methemoglobinemia, phosphate pollution, atmospheric pollution and the like. However, in spite of the negative impact of fertilizers on the environment, and the limits on fertilizer use, which have been legislated in several countries, the use of fertilizers is expected to increase in order to support food and fiber production for rapid population growth on limited land resources. For example, it has been estimated that by 2050, more than 150 million tons of nitrogenous fertilizer will be used worldwide annually.

Increased use efficiency of nitrogen by plants should enable crops to be cultivated with lower fertilizer input, or alternatively to be cultivated on soils of poorer quality and would therefore have significant economic impact in both developed and developing agricultural systems.

Genetic improvement of fertilizer use efficiency (FUE) in plants can be generated either via traditional breeding or via genetic engineering.

Attempts to generate plants with increased FUE have been described in U.S. Pat. Appl. Publication No. 20020046419 (U.S. Pat. No. 7,262,055 to Choo, et al.); U.S. Pat. Appl. No. 20050108791 to Edgerton et al.; U.S. Pat. Appl. No. 20060179511 to Chomet et al.; Good, A, et al. 2007 (Engineering nitrogen use efficiency with alanine aminotransferase. Canadian Journal of Botany 85: 252-262); and Good A G et al. 2004 (Trends Plant Sci. 9:597-605).

Yanagisawa et al. (Proc. Natl. Acad. Sci. U.S.A. 2004 101:7833-8) describe Dof1 transgenic plants which exhibit improved growth under low-nitrogen conditions.

U.S. Pat. No. 6,084,153 to Good et al. discloses the use of a stress responsive promoter to control the expression of Alanine Amine Transferase (AlaAT) and transgenic canola plants with improved drought and nitrogen deficiency tolerance when compared to control plants.

Yield is affected by various factors, such as, the number and size of the plant organs, plant architecture (for example, the number of branches), grains set length, number of filled grains, vigor (e.g. seedling), growth rate, root development, utilization of water, nutrients (e.g., nitrogen) and fertilizers, and stress tolerance.

Crops such as, corn, rice, wheat, canola and soybean account for over half of total human caloric intake, whether through direct consumption of the seeds themselves or through consumption of meat products raised on processed seeds or forage. Seeds are also a source of sugars, proteins and oils and metabolites used in industrial processes. The ability to increase plant yield, whether through increase dry matter accumulation rate, modifying cellulose or lignin composition, increase stalk strength, enlarge meristem size, change of plant branching pattern, erectness of leaves, increase in fertilization efficiency, enhanced seed dry matter accumulation rate, modification of seed development, enhanced seed filling or by increasing the content of oil, starch or protein in the seeds would have many applications in agricultural and non-agricultural uses such as in the biotechnological production of pharmaceuticals, antibodies or vaccines.

Vegetable or seed oils are the major source of energy and nutrition in human and animal diet. They are also used for the production of industrial products, such as paints, inks and lubricants. In addition, plant oils represent renewable sources of long-chain hydrocarbons which can be used as fuel. Since the currently used fossil fuels are finite resources and are gradually being depleted, fast growing biomass crops may be used as alternative fuels or for energy feedstocks and may reduce the dependence on fossil energy supplies. However, the major bottleneck for increasing consumption of plant oils as bio-fuel is the oil price, which is still higher than fossil fuel. In addition, the production rate of plant oil is limited by the availability of agricultural land and water. Thus, increasing plant oil yields from the same growing area can effectively overcome the shortage in production space and can decrease vegetable oil prices at the same time.

Studies aiming at increasing plant oil yields focus on the identification of genes involved in oil metabolism as well as in genes capable of increasing plant and seed yields in transgenic plants. Genes known to be involved in increasing plant oil yields include those participating in fatty acid synthesis or sequestering such as desaturase [e.g., DELTA6, DELTA12 or acyl-ACP (Ssi2; Arabidopsis Information Resource (TAIR; Hypertext Transfer Protocol://World Wide Web (dot) arabidopsis (dot) org/), TAIR No. AT2G43710)], OleosinA (TAIR No. AT3G01570) or FAD3 (TAIR No. AT2G29980), and various transcription factors and activators such as Led 1 [TAIR No. AT1G21970, Lotan et al. 1998. Cell. 26;93(7):1195-205], Lec2 [TAIR No. AT1G28300, Santos Mendoza et al. 2005, FEBS Lett. 579(21):4666-70], Fus3 (TAIR No. AT3G26790), ABI3 [TAIR No. AT3G24650, Lara et al. 2003. J Biol Chem. 278(23): 21003-11] and Wri1 [TAIR No. AT3G54320, Cernac and Benning, 2004. Plant J. 40(4): 575-85].

Genetic engineering efforts aiming at increasing oil content in plants (e.g., in seeds) include upregulating endoplasmic reticulum (FAD3) and plastidal (FAD7) fatty acid desaturases in potato (Zabrouskov V., et al., 2002; Physiol Plant. 116:172-185); over-expressing the GmDof4 and GmDof11 transcription factors (Wang H W et al., 2007; Plant J. 52:716-29); over-expressing a yeast glycerol-3-phosphate dehydrogenase under the control of a seed-specific promoter (Vigeolas H, et al. 2007, Plant Biotechnol J. 5:431-41; U.S. Pat. Appl. No. 20060168684); using Arabidopsis FAE1 and yeast SLC1-1 genes for improvements in erucic acid and oil content in rapeseed (Katavic V, et al., 2000, Biochem Soc Trans. 28:935-7).

Various patent applications disclose genes and proteins which can increase oil content in plants. These include for example, U.S. Pat. Appl. No. 20080076179 (lipid metabolism protein); U.S. Pat. Appl. No. 20060206961 (the Ypr140w polypeptide); U.S. Pat. Appl. No. 20060174373 [triacylglycerols synthesis enhancing protein (TEP)]; U.S. Pat. Appl. Nos. 20070169219, 20070006345, 20070006346 and 20060195943 (disclose transgenic plants with improved nitrogen use efficiency which can be used for the conversion into fuel or chemical feedstocks); WO2008/122980 (polynucleotides for increasing oil content, growth rate, biomass, yield and/or vigor of a plant).

Cotton and cotton by-products provide raw materials that are used to produce a wealth of consumer-based products in addition to textiles including cotton foodstuffs, livestock feed, fertilizer and paper. The production, marketing, consumption and trade of cotton-based products generate an excess of $100 billion annually in the U.S. alone, making cotton the number one value-added crop.

Even though 90% of cotton's value as a crop resides in the fiber (lint), yield and fiber quality has declined due to general erosion in genetic diversity of cotton varieties, and an increased vulnerability of the crop to environmental conditions.

There are many varieties of cotton plant, from which cotton fibers with a range of characteristics can be obtained and used for various applications. Cotton fibers may be characterized according to a variety of properties, some of which are considered highly desirable within the textile industry for the production of increasingly high quality products and optimal exploitation of modem spinning technologies. Commercially desirable properties include length, length uniformity, fineness, maturity ratio, decreased fuzz fiber production, micronaire, bundle strength, and single fiber strength. Much effort has been put into the improvement of the characteristics of cotton fibers mainly focusing on fiber length and fiber fineness. In particular, there is a great demand for cotton fibers of specific lengths.

A cotton fiber is composed of a single cell that has differentiated from an epidermal cell of the seed coat, developing through four stages, i.e., initiation, elongation, secondary cell wall thickening and maturation stages. More specifically, the elongation of a cotton fiber commences in the epidermal cell of the ovule immediately following flowering, after which the cotton fiber rapidly elongates for approximately 21 days. Fiber elongation is then terminated, and a secondary cell wall is formed and grown through maturation to become a mature cotton fiber.

Several candidate genes which are associated with the elongation, formation, quality and yield of cotton fibers were disclosed in various patent applications such as U.S. Pat. No. 5,880,100 and U.S. patent application Ser. Nos. 08/580,545, 08/867,484 and 09/262,653 (describing genes involved in cotton fiber elongation stage); WO0245485 (improving fiber quality by modulating sucrose synthase); U.S. Pat. No. 6,472,588 and WO0117333 (increasing fiber quality by transformation with a DNA encoding sucrose phosphate synthase); WO9508914 (using a fiber-specific promoter and a coding sequence encoding cotton peroxidase); WO9626639 (using an ovary specific promoter sequence to express plant growth modifying hormones in cotton ovule tissue, for altering fiber quality characteristics such as fiber dimension and strength); U.S. Pat. No. 5,981,834, U.S. Pat. No. 5,597,718, U.S. Pat. No. 5,620,882, U.S. Pat. No. 5,521,708 and U.S. Pat. No. 5,495,070 (coding sequences to alter the fiber characteristics of transgenic fiber producing plants); U.S. patent applications U.S. 2002049999 and U.S. 2003074697 (expressing a gene coding for endoxyloglucan transferase, catalase or peroxidase for improving cotton fiber characteristics); WO 01/40250 (improving cotton fiber quality by modulating transcription factor gene expression); WO 96/40924 (a cotton fiber transcriptional initiation regulatory region associated which is expressed in cotton fiber); EP0834566 (a gene which controls the fiber formation mechanism in cotton plant); WO2005/121364 (improving cotton fiber quality by modulating gene expression); WO2008/075364 (improving fiber quality, yield/biomass/vigor and/or abiotic stress tolerance of plants).

WO publication No. 2004/104162 discloses methods of increasing abiotic stress tolerance and/or biomass in plants and plants generated thereby.

WO publication No. 2004/111183 discloses nucleotide sequences for regulating gene expression in plant trichomes and constructs and methods utilizing same.

WO publication No. 2004/081173 discloses novel plant derived regulatory sequences and constructs and methods of using such sequences for directing expression of exogenous polynucleotide sequences in plants.

WO publication No. 2005/121364 discloses polynucleotides and polypeptides involved in plant fiber development and methods of using same for improving fiber quality, yield and/or biomass of a fiber producing plant.

WO publication No. 2007/049275 discloses isolated polypeptides, polynucleotides encoding same, transgenic plants expressing same and methods of using same for increasing fertilizer use efficiency, plant abiotic stress tolerance and biomass.

WO publication No. 2007/020638 discloses methods of increasing abiotic stress tolerance and/or biomass in plants and plants generated thereby.

WO publication No. 2008/122980 discloses genes constructs and methods for increasing oil content, growth rate and biomass of plants.

WO publication No. 2008/075364 discloses polynucleotides involved in plant fiber development and methods of using same.

WO publication No. 2009/083958 discloses methods of increasing water use efficiency, fertilizer use efficiency, biotic/abiotic stress tolerance, yield and biomass in plant and plants generated thereby.

WO publication No. 2009/141824 discloses isolated polynucleotides and methods using same for increasing plant utility.

WO publication No. 2009/013750 discloses genes, constructs and methods of increasing abiotic stress tolerance, biomass and/or yield in plants generated thereby.

WO publication No. 2010/020941 discloses methods of increasing nitrogen use efficiency, abiotic stress tolerance, yield and biomass in plants and plants generated thereby.

WO publication No. 2010/076756 discloses isolated polynucleotides for increasing abiotic stress tolerance, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, and/or nitrogen use efficiency of a plant.

WO2010/100595 publication discloses isolated polynucleotides and polypeptides, and methods of using same for increasing plant yield and/or agricultural characteristics.

WO publication No. 2010/049897 discloses isolated polynucleotides and polypeptides and methods of using same for increasing plant yield, biomass, growth rate, vigor, oil content, abiotic stress tolerance of plants and nitrogen use efficiency.

WO2010/143138 publication discloses isolated polynucleotides and polypeptides, and methods of using same for increasing nitrogen use efficiency, fertilizer use efficiency, yield, growth rate, vigor, biomass, oil content, abiotic stress tolerance and/or water use efficiency

WO publication No. 2011/080674 discloses isolated polynucleotides and polypeptides and methods of using same for increasing plant yield, biomass, growth rate, vigor, oil content, abiotic stress tolerance of plants and nitrogen use efficiency.

WO2011/015985 publication discloses polynucleotides and polypeptides for increasing desirable plant qualities.

WO2011/135527 publication discloses isolated polynucleotides and polypeptides for increasing plant yield and/or agricultural characteristics.

WO2012/028993 publication discloses isolated polynucleotides and polypeptides, and methods of using same for increasing nitrogen use efficiency, yield, growth rate, vigor, biomass, oil content, and/or abiotic stress tolerance.

WO2012/085862 publication discloses isolated polynucleotides and polypeptides, and methods of using same for improving plant properties.

WO2012/150598 publication discloses isolated polynucleotides and polypeptides and methods of using same for increasing plant yield, biomass, growth rate, vigor, oil content, abiotic stress tolerance of plants and nitrogen use efficiency.

WO2013/027223 publication discloses isolated polynucleotides and polypeptides, and methods of using same for increasing plant yield and/or agricultural characteristics.

WO2013/080203 publication discloses isolated polynucleotides and polypeptides, and methods of using same for increasing nitrogen use efficiency, yield, growth rate, vigor, biomass, oil content, and/or abiotic stress tolerance.

WO2013/098819 publication discloses isolated polynucleotides and polypeptides, and methods of using same for increasing yield of plants.

SUMMARY OF THE INVENTION

According to an aspect of some embodiments of the present invention there is provided a method of increasing yield, growth rate, biomass, vigor, oil content, seed yield, fiber yield, fiber quality, fiber length, photosynthetic capacity, nitrogen use efficiency, and/or abiotic stress tolerance of a plant, comprising expressing within the plant an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide at least 80% identical to SEQ ID NO: 475-770, 6179-9796, 9798-10420 or 10421, thereby increasing the yield, growth rate, biomass, vigor, oil content, seed yield, fiber yield, fiber quality, fiber length, photosynthetic capacity, nitrogen use efficiency, and/or abiotic stress tolerance of the plant.

According to an aspect of some embodiments of the present invention there is provided a method of increasing yield, growth rate, biomass, vigor, oil content, seed yield, fiber yield, fiber quality, fiber length, photosynthetic capacity, nitrogen use efficiency, and/or abiotic stress tolerance of a plant, comprising expressing within the plant an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide selected from the group consisting of SEQ ID NOs: 475-770 and 6179-10421, thereby increasing the yield, growth rate, biomass, vigor, oil content, seed yield, fiber yield, fiber quality, fiber length, photosynthetic capacity, nitrogen use efficiency, and/or abiotic stress tolerance of the plant.

According to an aspect of some embodiments of the present invention there is provided a method of producing a crop comprising growing a crop plant transformed with an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide at least 80% homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs: 475-770, 6179-9796, and 9798-10421, wherein the crop plant is derived from plants selected for increased yield, increased growth rate, increased biomass, increased vigor, increased oil content, increased seed yield, increased fiber yield, increased fiber quality, increased fiber length, increased photosynthetic capacity, increased nitrogen use efficiency, and/or increased abiotic stress tolerance as compared to a wild type plant of the same species which is grown under the same growth conditions, and the crop plant having the increased yield, increased growth rate, increased biomass, increased vigor, increased oil content, increased seed yield, increased fiber yield, increased fiber quality, increased fiber length, increased photosynthetic capacity, increased nitrogen use efficiency, and/or increased abiotic stress tolerance, thereby producing the crop.

According to an aspect of some embodiments of the present invention there is provided a method of increasing yield, growth rate, biomass, vigor, oil content, seed yield, fiber yield, fiber quality, fiber length, photosynthetic capacity, nitrogen use efficiency, and/or abiotic stress tolerance of a plant, comprising expressing within the plant an exogenous polynucleotide comprising a nucleic acid sequence at least 80% identical to SEQ ID NO: 1-474, 771-6177 or 6178, thereby increasing the yield, growth rate, biomass, vigor, oil content, seed yield, fiber yield, fiber quality, fiber length, photosynthetic capacity, nitrogen use efficiency, and/or abiotic stress tolerance of the plant.

According to an aspect of some embodiments of the present invention there is provided a method of increasing yield, growth rate, biomass, vigor, oil content, seed yield, fiber yield, fiber quality, fiber length, photosynthetic capacity, nitrogen use efficiency, and/or abiotic stress tolerance of a plant, comprising expressing within the plant an exogenous polynucleotide comprising the nucleic acid sequence selected from the group consisting of SEQ ID NOs:1-474 and 771-6178, thereby increasing the yield, growth rate, biomass, vigor, oil content, seed yield, fiber yield, fiber quality, fiber length, photosynthetic capacity, nitrogen use efficiency, and/or abiotic stress tolerance of the plant.

According to an aspect of some embodiments of the present invention there is provided a method of producing a crop comprising growing a crop plant transformed with an exogenous polynucleotide which comprises a nucleic acid sequence which is at least 80% identical to the nucleic acid sequence selected from the group consisting of SEQ ID NOs:1-474 and 771-6178, wherein the crop plant is derived from plants selected for increased yield, increased growth rate, increased biomass, increased vigor, increased oil content, increased seed yield, increased fiber yield, increased fiber quality, increased fiber length, increased photosynthetic capacity, increased nitrogen use efficiency, and/or increased abiotic stress tolerance as compared to a wild type plant of the same species which is grown under the same growth conditions, and the crop plant having the increased yield, increased growth rate, increased biomass, increased vigor, increased oil content, increased seed yield, increased fiber yield, increased fiber quality, increased fiber length, increased photosynthetic capacity, increased nitrogen use efficiency, and/or increased abiotic stress tolerance, thereby producing the crop.

According to an aspect of some embodiments of the present invention there is provided an isolated polynucleotide comprising a nucleic acid sequence encoding a polypeptide which comprises an amino acid sequence at least 80% homologous to the amino acid sequence set forth in SEQ ID NO:475-770, 6179-9796, 9798-10420 or 10421, wherein said amino acid sequence is capable of increasing yield, growth rate, biomass, vigor, oil content, seed yield, fiber yield, fiber quality, fiber length, photosynthetic capacity, nitrogen use efficiency, and/or abiotic stress tolerance of a plant.

According to an aspect of some embodiments of the present invention there is provided an isolated polynucleotide comprising a nucleic acid sequence at least 80% identical to SEQ ID NOs: 1-474 and 771-6178, wherein said nucleic acid sequence is capable of increasing yield, growth rate, biomass, vigor, oil content, seed yield, fiber yield, fiber quality, fiber length, photosynthetic capacity, nitrogen use efficiency, and/or abiotic stress tolerance of a plant.

According to an aspect of some embodiments of the present invention there is provided an isolated polynucleotide comprising the nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-474 and 771-6178.

According to an aspect of some embodiments of the present invention there is provided a nucleic acid construct comprising the isolated polynucleotide of some embodiments of the invention, and a promoter for directing transcription of said nucleic acid sequence in a host cell.

According to an aspect of some embodiments of the present invention there is provided an isolated polypeptide comprising an amino acid sequence at least 80% homologous to SEQ ID NO: 475-770, 6179-9796, 9798-10420 or 10421, wherein said amino acid sequence is capable of increasing yield, growth rate, biomass, vigor, oil content, seed yield, fiber yield, fiber quality, fiber length, photosynthetic capacity, nitrogen use efficiency, and/or abiotic stress tolerance of a plant.

According to an aspect of some embodiments of the present invention there is provided an isolated polypeptide comprising the amino acid sequence selected from the group consisting of SEQ ID NOs: 475-770 and 6179-10421.

According to an aspect of some embodiments of the present invention there is provided a plant cell exogenously expressing the polynucleotide of some embodiments of the invention, or the nucleic acid construct of some embodiments of the invention.

According to an aspect of some embodiments of the present invention there is provided a plant cell exogenously expressing the polypeptide of some embodiments of the invention.

According to some embodiments of the invention, the nucleic acid sequence encodes an amino acid sequence selected from the group consisting of SEQ ID NOs: 475-770 and 6179-10421.

According to some embodiments of the invention, the nucleic acid sequence is selected from the group consisting of SEQ ID NOs: 1-474 and 771-6178.

According to some embodiments of the invention, the polynucleotide consists of the nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-474 and 771-6178.

According to some embodiments of the invention, the nucleic acid sequence encodes the amino acid sequence selected from the group consisting of SEQ ID NOs: 475-770 and 6179-10421.

According to some embodiments of the invention, the plant cell forms part of a plant.

According to some embodiments of the invention, the method further comprising growing the plant expressing said exogenous polynucleotide under the abiotic stress.

According to some embodiments of the invention, the abiotic stress is selected from the group consisting of salinity, drought, osmotic stress, water deprivation, flood, low temperature, high temperature, heavy metal toxicity, anaerobiosis, nutrient deficiency, nitrogen deficiency, nutrient excess, atmospheric pollution and UV irradiation.

According to some embodiments of the invention, the yield comprises seed yield or oil yield.

According to an aspect of some embodiments of the present invention there is provided a transgenic plant comprising the nucleic acid construct of some embodiments of the invention or the plant cell of some embodiments of the invention.

According to some embodiments of the invention, the method further comprising growing the plant expressing said exogenous polynucleotide under nitrogen-limiting conditions.

According to some embodiments of the invention, the promoter is heterologous to said isolated polynucleotide and/or to said host cell.

According to an aspect of some embodiments of the present invention there is provided a method of growing a crop, the method comprising seeding seeds and/or planting plantlets of a plant transformed with the isolated polynucleotide of some embodiments of the invention, or with the nucleic acid construct of some embodiments of the invention, wherein the plant is derived from plants selected for at least one trait selected from the group consisting of: increased nitrogen use efficiency, increased photosynthetic capacity, increased abiotic stress tolerance, increased biomass, increased growth rate, increased vigor, increased yield, increased fiber yield, increased fiber quality, increased fiber length, and increased oil content as compared to a non-transformed plant, thereby growing the crop.

According to some embodiments of the invention, the non-transformed plant is a wild type plant of identical genetic background.

According to some embodiments of the invention, the non-transformed plant is a wild type plant of the same species.

According to some embodiments of the invention, the non-transformed plant is grown under identical growth conditions.

According to an aspect of some embodiments of the present invention there is provided a method of producing a crop comprising growing a crop of a plant transformed with an exogenous polynucleotide encoding a polypeptide at least 80% homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs: 475-770, 6179-9796, 9798-10420 or 10421, wherein the crop plant is derived from plants selected for increased yield, increased growth rate, increased biomass, increased vigor, increased oil content, increased seed yield, increased fiber yield, increased fiber quality, increased fiber length, increased photosynthetic capacity, increased nitrogen use efficiency, and/or increased abiotic stress tolerance as compared to a wild type plant of the same species which is grown under the same growth conditions, and the crop plant having the increased yield, increased growth rate, increased biomass, increased vigor, increased oil content, increased seed yield, increased fiber yield, increased fiber quality, increased fiber length, increased photosynthetic capacity, increased nitrogen use efficiency, and/or increased abiotic stress tolerance, thereby producing the crop.

According to an aspect of some embodiments of the present invention there is provided a method of producing a crop comprising growing a crop of a plant transformed with an exogenous polynucleotide encoding the polypeptide selected from the group consisting of SEQ ID NOs: 475-770 and 6179-10421, wherein the crop plant is derived from plants selected for increased yield, increased growth rate, increased biomass, increased vigor, increased oil content, increased seed yield, increased fiber yield, increased fiber quality, increased fiber length, increased photosynthetic capacity, increased nitrogen use efficiency, and/or increased abiotic stress tolerance as compared to a wild type plant of the same species which is grown under the same growth conditions, and the crop plant having the increased yield, increased growth rate, increased biomass, increased vigor, increased oil content, increased seed yield, increased fiber yield, increased fiber quality, increased fiber length, increased photosynthetic capacity, increased nitrogen use efficiency, and/or increased abiotic stress tolerance, thereby producing the crop.

According to an aspect of some embodiments of the present invention there is provided a method of growing a crop, the method comprising seeding seeds and/or planting plantlets of a plant transformed with the isolated polynucleotide of some embodiments of the invention, or the nucleic acid construct of some embodiments of the invention, wherein the plant is derived from plants selected for at least one trait selected from the group consisting of: increased nitrogen use efficiency, increased photosynthetic capacity, increased abiotic stress tolerance, increased biomass, increased growth rate, increased vigor, increased yield, increased fiber yield, increased fiber quality, increased fiber length, and increased oil content as compared to a non-transformed plant, thereby growing the crop.

Unless otherwise defined, all technical and/or scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the invention pertains. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of embodiments of the invention, exemplary methods and/or materials are described below. In case of conflict, the patent specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and are not intended to be necessarily limiting.

BRIEF DESCRIPTION OF THE DRAWINGS

Some embodiments of the invention are herein described, by way of example only, with reference to the accompanying drawings. With specific reference now to the drawings in detail, it is stressed that the particulars shown are by way of example and for purposes of illustrative discussion of embodiments of the invention. In this regard, the description taken with the drawings makes apparent to those skilled in the art how embodiments of the invention may be practiced.

In the drawings:

FIG. 1 is a schematic illustration of the modified pGI binary plasmid containing the new At6669 promoter (SEQ ID NO: 10446) and the GUSintron (pQYN 6669) used for expressing the isolated polynucleotide sequences of the invention. RB—T-DNA right border; LB—T-DNA left border; MCS—Multiple cloning site; RE—any restriction enzyme; NOS pro=nopaline synthase promoter; NPT-II=neomycin phosphotransferase gene; NOS ter=nopaline synthase terminator; Poly-A signal (polyadenylation signal); GUSintron—the GUS reporter gene (coding sequence and intron). The isolated polynucleotide sequences of the invention were cloned into the vector while replacing the GUSintron reporter gene.

FIG. 2 is a schematic illustration of the modified pGI binary plasmid containing the new At6669 promoter (SEQ ID NO: 10446) (pQFN or pQFNc) used for expressing the isolated polynucleotide sequences of the invention. RB—T-DNA right border; LB—T-DNA left border; MCS—Multiple cloning site; RE—any restriction enzyme; NOS pro=nopaline synthase promoter (SEQ ID NO:10457); NPT-II=neomycin phosphotransferase gene; NOS ter=nopaline synthase terminator; Poly-A signal (polyadenylation signal); The isolated polynucleotide sequences of the invention were cloned into the MCS of the vector.

FIGS. 3A-F are images depicting visualization of root development of transgenic plants exogenously expressing the polynucleotide of some embodiments of the invention when grown in transparent agar plates under normal (FIGS. 3A-B), osmotic stress (15% PEG; FIGS. 3C-D) or nitrogen-limiting (FIGS. 3E-F) conditions. The different transgenes were grown in transparent agar plates for 17 days (7 days nursery and 10 days after transplanting). The plates were photographed every 3-4 days starting at day 1 after transplanting. FIG. 3A—An image of a photograph of plants taken following 10 after transplanting days on agar plates when grown under normal (standard) conditions. FIG. 3B—An image of root analysis of the plants shown in FIG. 3A in which the lengths of the roots measured are represented by arrows. FIG. 3C—An image of a photograph of plants taken following 10 days after transplanting on agar plates, grown under high osmotic (PEG 15%) conditions. FIG. 3D—An image of root analysis of the plants shown in FIG. 3C in which the lengths of the roots measured are represented by arrows. FIG. 3E—An image of a photograph of plants taken following 10 days after transplanting on agar plates, grown under low nitrogen conditions. FIG. 3F—An image of root analysis of the plants shown in FIG. 3E in which the lengths of the roots measured are represented by arrows.

FIG. 4 is a schematic illustration of the modified pGI binary plasmid containing the Root Promoter (pQNa RP) used for expressing the isolated polynucleotide sequences of the invention. RB—T-DNA right border; LB—T-DNA left border; NOS pro=nopaline synthase promoter; NPT-II=neomycin phosphotransferase gene; NOS ter=nopaline synthase terminator; Poly-A signal (polyadenylation signal); The isolated polynucleotide sequences according to some embodiments of the invention were cloned into the MCS (Multiple cloning site) of the vector.

FIG. 5 is a schematic illustration of the pQYN plasmid.

FIG. 6 is a schematic illustration of the pQFN plasmid.

FIG. 7 is a schematic illustration of the pQFYN plasmid.

FIG. 8 is a schematic illustration of the modified pGI binary plasmid (pQXNc) used for expressing the isolated polynucleotide sequences of some embodiments of the invention. RB—T-DNA right border; LB—T-DNA left border; NOS pro=nopaline synthase promoter; NPT-II=neomycin phosphotransferase gene; NOS ter=nopaline synthase terminator; RE=any restriction enzyme; Poly-A signal (polyadenylation signal); 35S—the 35S promoter (pQFNC; SEQ ID NO: 10442). The isolated polynucleotide sequences of some embodiments of the invention were cloned into the MCS (Multiple cloning site) of the vector.

DESCRIPTION OF SPECIFIC EMBODIMENTS OF THE INVENTION

The present invention, in some embodiments thereof, relates to isolated polypeptides and polynucleotides, nucleic acid constructs comprising the isolated polypeptides, transgenic plants expressing same and methods of using same for increasing abiotic stress tolerance (ABST), water use efficiency (WUE), yield (e.g., grain quantity and/or quality), fiber yield, fiber quality, biomass, oil content, growth rate, photosynthetic capacity, vigor, nitrogen use efficiency (NUE) and/or fertilizer use efficiency (FUE) of a plant.

Before explaining at least one embodiment of the invention in detail, it is to be understood that the invention is not necessarily limited in its application to the details set forth in the following description or exemplified by the Examples. The invention is capable of other embodiments or of being practiced or carried out in various ways.

The present inventors have identified novel polypeptides and polynucleotides which can be used to generate nucleic acid constructs and transgenic plants and to increase abiotic stress tolerance, water use efficiency, yield (e.g., seed yield), growth rate, photosynthetic capacity, vigor, biomass, oil content, fiber yield, fiber quality, fiber length, nitrogen use efficiency, and/or fertilizer use efficiency of a plant.

As shown in the Examples section which follows, the present inventors have utilized bioinformatics tools to identify polynucleotides and polypeptides which enhance abiotic stress tolerance, yield (e.g., seed yield, oil yield, oil content), photosynthetic capacity, growth rate, biomass, vigor, fiber quality and yield and/or fertilizer (e.g., nitrogen) use efficiency of a plant. Genes which affect the trait-of-interest were identified (SEQ ID NOs: 475-770 for polypeptides; and SEQ ID NOs: 1-474 for polynucleotides) based on expression profiles of genes of several Sorghum varieties (Examples 2-5 in the Examples section which follows), Maize hybrids (Examples 6-9 in the Examples section which follows), Foxtail Millet varieties (Example 10 in the Examples section which follows), Barley accessions (Examples 11-12 in the Examples section which follows), tomato varieties (Example 13 in the Examples section which follows), Soybean varieties (Example 14 in the Examples section which follows), Cotton lines (Example 15 in the Examples section which follows), and Arabidopsis ecotypes and tissues (Examples 16 and 17 in the Examples section which follows), homology with genes known to affect the trait-of-interest and using digital expression profile in specific tissues and conditions (Tables 1-151, Examples 1-18 in the Examples section which follows). Homologous (e.g., orthologs) polypeptides and polynucleotides having the same function were also identified (SEQ ID NOs: 6179-10421 for polypeptides, and SEQ ID NOs: 771-6178 for polynucleotides; Table 152, Example 19 in the Examples section which follows). Transgenic plants over-expressing the identified polynucleotides and polypeptides were generated (Examples 20-22 in the Examples section which follows) and were found to exhibit increased tolerance to abiotic stress conditions (e.g., drought stress, salinity stress, osmotic stress), increased yield (e.g., seed yield, weight of 1000 seeds, harvest index), increased biomass (e.g., fresh and dry weight, rosette area), increased vigor and growth rate (e.g., relative growth rate), increased photosynthetic capacity (e.g., leaf area, leaves number), drought avoidance (e.g., early flowering and inflorescence emergence), and increased nitrogen use efficiency (Tables 154-181, Examples 23-25 in the Examples section which follows) as compared to control plants grown under the same growth conditions. Altogether, these results suggest the use of the novel polynucleotides and polypeptides of the invention [e.g., SEQ ID NOs:475-770 and 6179-10421 (for polypeptides) and SEQ ID NOs: 1-474 and 771-6178 (for polynucleotides)] for increasing yield (e.g., oil yield, seed yield and oil content), growth rate, biomass, vigor, fiber yield, fiber quality, fiber length, photosynthetic capacity, nitrogen use efficiency, fertilizer use efficiency, water use efficiency and/or abiotic stress tolerance of a plant.

Thus, according to an aspect of some embodiments of the invention, there is provided method of increasing abiotic stress tolerance, water use efficiency, growth rate, vigor, biomass, oil content, yield, seed yield, fiber yield, fiber quality, fiber length, photosynthetic capacity, and/or fertilizer use efficiency (e.g., nitrogen use efficiency) of a plant, comprising expressing within the plant an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or more say 100% homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs: 475-770 and 6179-10421, thereby increasing the abiotic stress tolerance, water use efficiency, growth rate, vigor, biomass, oil content, yield, seed yield, fiber yield, fiber quality, fiber length, photosynthetic capacity, and/or fertilizer use efficiency (e.g., nitrogen use efficiency) of the plant.

As used herein the phrase “plant yield” refers to the amount (e.g., as determined by weight or size) or quantity (numbers) of tissues or organs produced per plant or per growing season. Hence increased yield could affect the economic benefit one can obtain from the plant in a certain growing area and/or growing time.

It should be noted that a plant yield can be affected by various parameters including, but not limited to, plant biomass; plant vigor; growth rate; seed yield; seed or grain quantity; seed or grain quality; oil yield; content of oil, starch and/or protein in harvested organs (e.g., seeds or vegetative parts of the plant); number of flowers (florets) per panicle (expressed as a ratio of number of filled seeds over number of primary panicles); harvest index; number of plants grown per area; number and size of harvested organs per plant and per area; number of plants per growing area (density); number of harvested organs in field; total leaf area; carbon assimilation and carbon partitioning (the distribution/allocation of carbon within the plant); resistance to shade; number of harvestable organs (e.g. seeds), seeds per pod, weight per seed; and modified architecture [such as increase stalk diameter, thickness or improvement of physical properties (e.g. elasticity)].

As used herein the phrase “seed yield” refers to the number or weight of the seeds per plant, seeds per pod, or per growing area or to the weight of a single seed, or to the oil extracted per seed. Hence, seed yield can be affected by seed dimensions (e.g., length, width, perimeter, area and/or volume), number of (filled) seeds and seed filling rate and by seed oil content. Hence increase seed yield per plant could affect the economic benefit one can obtain from the plant in a certain growing area and/or growing time; and increase seed yield per growing area could be achieved by increasing seed yield per plant, and/or by increasing number of plants grown on the same given area.

The term “seed” (also referred to as “grain” or “kernel”) as used herein refers to a small embryonic plant enclosed in a covering called the seed coat (usually with some stored food), the product of the ripened ovule of gymnosperm and angiosperm plants which occurs after fertilization and some growth within the mother plant.

The phrase “oil content” as used herein refers to the amount of lipids in a given plant organ, either the seeds (seed oil content) or the vegetative portion of the plant (vegetative oil content) and is typically expressed as percentage of dry weight (10% humidity of seeds) or wet weight (for vegetative portion).

It should be noted that oil content is affected by intrinsic oil production of a tissue (e.g., seed, vegetative portion), as well as the mass or size of the oil-producing tissue per plant or per growth period.

In one embodiment, increase in oil content of the plant can be achieved by increasing the size/mass of a plant's tissue(s) which comprise oil per growth period. Thus, increased oil content of a plant can be achieved by increasing the yield, growth rate, biomass and vigor of the plant.

As used herein the phrase “plant biomass” refers to the amount (e.g., measured in grams of air-dry tissue) of a tissue produced from the plant in a growing season, which could also determine or affect the plant yield or the yield per growing area. An increase in plant biomass can be in the whole plant or in parts thereof such as aboveground (harvestable) parts, vegetative biomass, roots and seeds.

As used herein the phrase “growth rate” refers to the increase in plant organ/tissue size per time (can be measured in cm²per day or cm/day).

As used herein the phrase “photosynthetic capacity” (also known as “A”) refers to a measure of the maximum rate at which leaves are able to fix carbon during photosynthesis. It is typically measured as the amount of carbon dioxide that is fixed per square meter per second, for example as μmol m⁻²sec⁻¹. Plants are able to increase their photosynthetic capacity by several modes of action, such as by increasing the total leaves area (e.g., by increase of leaves area, increase in the number of leaves, and increase in plant's vigor, e.g., the ability of the plant to grow new leaves along time in course) as well as by increasing the ability of the plant to efficiently execute carbon fixation in the leaves. Hence, the increase in total leaves area can be used as a reliable measurement parameter for photosynthetic capacity increment.

As used herein the phrase “plant vigor” refers to the amount (measured by weight) of tissue produced by the plant in a given time. Hence increased vigor could determine or affect the plant yield or the yield per growing time or growing area. In addition, early vigor (seed and/or seedling) results in improved field stand.

Improving early vigor is an important objective of modern rice breeding programs in both temperate and tropical rice cultivars. Long roots are important for proper soil anchorage in water-seeded rice. Where rice is sown directly into flooded fields, and where plants must emerge rapidly through water, longer shoots are associated with vigor. Where drill-seeding is practiced, longer mesocotyls and coleoptiles are important for good seedling emergence. The ability to engineer early vigor into plants would be of great importance in agriculture. For example, poor early vigor has been a limitation to the introduction of maize (Zea mays L.) hybrids based on Corn Belt germplasm in the European Atlantic.

It should be noted that a plant yield can be determined under stress (e.g., abiotic stress, nitrogen-limiting conditions) and/or non-stress (normal) conditions.

As used herein, the phrase “non-stress conditions” refers to the growth conditions (e.g., water, temperature, light-dark cycles, humidity, salt concentration, fertilizer concentration in soil, nutrient supply such as nitrogen, phosphorous and/or potassium), that do not significantly go beyond the everyday climatic and other abiotic conditions that plants may encounter, and which allow optimal growth, metabolism, reproduction and/or viability of a plant at any stage in its life cycle (e.g., in a crop plant from seed to a mature plant and back to seed again). Persons skilled in the art are aware of normal soil conditions and climatic conditions for a given plant in a given geographic location. It should be noted that while the non-stress conditions may include some mild variations from the optimal conditions (which vary from one type/species of a plant to another), such variations do not cause the plant to cease growing without the capacity to resume growth.

The phrase “abiotic stress” as used herein refers to any adverse effect on metabolism, growth, reproduction and/or viability of a plant. Accordingly, abiotic stress can be induced by suboptimal environmental growth conditions such as, for example, salinity, osmotic stress, water deprivation, drought, flooding, freezing, low or high temperature, heavy metal toxicity, anaerobiosis, nutrient deficiency (e.g., nitrogen deficiency or limited nitrogen), atmospheric pollution or UV irradiation. The implications of abiotic stress are discussed in the Background section.

The phrase “abiotic stress tolerance” as used herein refers to the ability of a plant to endure an abiotic stress without suffering a substantial alteration in metabolism, growth, productivity and/or viability.

Plants are subject to a range of environmental challenges. Several of these, including salt stress, general osmotic stress, drought stress and freezing stress, have the ability to impact whole plant and cellular water availability. Not surprisingly, then, plant responses to this collection of stresses are related. Zhu (2002) Ann. Rev. Plant Biol. 53: 247-273 et al. note that “most studies on water stress signaling have focused on salt stress primarily because plant responses to salt and drought are closely related and the mechanisms overlap”. Many examples of similar responses and pathways to this set of stresses have been documented. For example, the CBF transcription factors have been shown to condition resistance to salt, freezing and drought (Kasuga et al. (1999) Nature Biotech. 17: 287-291). The Arabidopsis rd29B gene is induced in response to both salt and dehydration stress, a process that is mediated largely through an ABA signal transduction process (Uno et al. (2000) Proc. Natl. Acad. Sci. USA 97: 11632-11637), resulting in altered activity of transcription factors that bind to an upstream element within the rd29B promoter. In Mesembryanthemum crystallinum (ice plant), Patharker and Cushman have shown that a calcium-dependent protein kinase (McCDPK1) is induced by exposure to both drought and salt stresses (Patharker and Cushman (2000) Plant J. 24: 679-691). The stress-induced kinase was also shown to phosphorylate a transcription factor, presumably altering its activity, although transcript levels of the target transcription factor are not altered in response to salt or drought stress. Similarly, Saijo et al. demonstrated that a rice salt/drought-induced calmodulin-dependent protein kinase (OsCDPK7) conferred increased salt and drought tolerance to rice when overexpressed (Saijo et al. (2000) Plant J. 23: 319-327).

Exposure to dehydration invokes similar survival strategies in plants as does freezing stress (see, for example, Yelenosky (1989) Plant Physiol 89: 444-451) and drought stress induces freezing tolerance (see, for example, Siminovitch et al. (1982) Plant Physiol 69: 250-255; and Guy et al. (1992) Planta 188: 265-270). In addition to the induction of cold-acclimation proteins, strategies that allow plants to survive in low water conditions may include, for example, reduced surface area, or surface oil or wax production. In another example increased solute content of the plant prevents evaporation and water loss due to heat, drought, salinity, osmoticum, and the like therefore providing a better plant tolerance to the above stresses.

It will be appreciated that some pathways involved in resistance to one stress (as described above), will also be involved in resistance to other stresses, regulated by the same or homologous genes. Of course, the overall resistance pathways are related, not identical, and therefore not all genes controlling resistance to one stress will control resistance to the other stresses. Nonetheless, if a gene conditions resistance to one of these stresses, it would be apparent to one skilled in the art to test for resistance to these related stresses. Methods of assessing stress resistance are further provided in the Examples section which follows.

As used herein the phrase “water use efficiency (WUE)” refers to the level of organic matter produced per unit of water consumed by the plant, i.e., the dry weight of a plant in relation to the plant's water use, e.g., the biomass produced per unit transpiration.

As used herein the phrase “fertilizer use efficiency” refers to the metabolic process(es) which lead to an increase in the plant's yield, biomass, vigor, and growth rate per fertilizer unit applied. The metabolic process can be the uptake, spread, absorbent, accumulation, relocation (within the plant) and use of one or more of the minerals and organic moieties absorbed by the plant, such as nitrogen, phosphates and/or potassium.

As used herein the phrase “fertilizer-limiting conditions” refers to growth conditions which include a level (e.g., concentration) of a fertilizer applied which is below the level needed for normal plant metabolism, growth, reproduction and/or viability.

As used herein the phrase “nitrogen use efficiency (NUE)” refers to the metabolic process(es) which lead to an increase in the plant's yield, biomass, vigor, and growth rate per nitrogen unit applied. The metabolic process can be the uptake, spread, absorbent, accumulation, relocation (within the plant) and use of nitrogen absorbed by the plant.

As used herein the phrase “nitrogen-limiting conditions” refers to growth conditions which include a level (e.g., concentration) of nitrogen (e.g., ammonium or nitrate) applied which is below the level needed for normal plant metabolism, growth, reproduction and/or viability.

Improved plant NUE and FUE is translated in the field into either harvesting similar quantities of yield, while implementing less fertilizers, or increased yields gained by implementing the same levels of fertilizers. Thus, improved NUE or FUE has a direct effect on plant yield in the field. Thus, the polynucleotides and polypeptides of some embodiments of the invention positively affect plant yield, seed yield, and plant biomass. In addition, the benefit of improved plant NUE will certainly improve crop quality and biochemical constituents of the seed such as protein yield and oil yield.

It should be noted that improved AB ST will confer plants with improved vigor also under non-stress conditions, resulting in crops having improved biomass and/or yield e.g., elongated fibers for the cotton industry, higher oil content.

The term “fiber” is usually inclusive of thick-walled conducting cells such as vessels and tracheids and to fibrillar aggregates of many individual fiber cells. Hence, the term “fiber” refers to (a) thick-walled conducting and non-conducting cells of the xylem; (b) fibers of extraxylary origin, including those from phloem, bark, ground tissue, and epidermis; and (c) fibers from stems, leaves, roots, seeds, and flowers or inflorescences (such as those of Sorghum vulgare used in the manufacture of brushes and brooms).

Example of fiber producing plants, include, but are not limited to, agricultural crops such as cotton, silk cotton tree (Kapok, Ceiba pentandra), desert willow, creosote bush, winterfat, balsa, kenaf, roselle, jute, sisal abaca, flax, corn, sugar cane, hemp, ramie, kapok, coir, bamboo, Spanish moss and Agave spp. (e.g. sisal).

As used herein the phrase “fiber quality” refers to at least one fiber parameter which is agriculturally desired, or required in the fiber industry (further described hereinbelow). Examples of such parameters, include but are not limited to, fiber length, fiber strength, fiber fitness, fiber weight per unit length, maturity ratio and uniformity (further described hereinbelow).

Cotton fiber (lint) quality is typically measured according to fiber length, strength and fineness. Accordingly, the lint quality is considered higher when the fiber is longer, stronger and finer.

As used herein the phrase “fiber yield” refers to the amount or quantity of fibers produced from the fiber producing plant.

As used herein the term “increasing” refers to at least about 2%, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, increase in abiotic stress tolerance, water use efficiency, yield (e.g., seed yield), growth rate, vigor, biomass, oil content, fiber yield, fiber quality, fiber length, photosynthetic capacity, nitrogen use efficiency, and/or fertilizer use efficiency of a plant as compared to a native plant or a wild type plant [i.e., a plant not modified with the biomolecules (polynucleotide or polypeptides) of the invention, e.g., a non-transformed plant of the same species which is grown under the same (e.g., identical) growth conditions].

The phrase “expressing within the plant an exogenous polynucleotide” as used herein refers to upregulating the expression level of an exogenous polynucleotide within the plant by introducing the exogenous polynucleotide into a plant cell or plant and expressing by recombinant means, as further described herein below.

As used herein “expressing” refers to expression at the mRNA and optionally polypeptide level.

As used herein, the phrase “exogenous polynucleotide” refers to a heterologous nucleic acid sequence which may not be naturally expressed within the plant (e.g., a nucleic acid sequence from a different species) or which overexpression in the plant is desired. The exogenous polynucleotide may be introduced into the plant in a stable or transient manner, so as to produce a ribonucleic acid (RNA) molecule and/or a polypeptide molecule. It should be noted that the exogenous polynucleotide may comprise a nucleic acid sequence which is identical or partially homologous to an endogenous nucleic acid sequence of the plant.

The term “endogenous” as used herein refers to any polynucleotide or polypeptide which is present and/or naturally expressed within a plant or a cell thereof.

According to some embodiments of the invention, the exogenous polynucleotide of the invention comprises a nucleic acid sequence encoding a polypeptide having an amino acid sequence at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or more say 100% homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs:475-770 and 6179-10421.

Homologous sequences include both orthologous and paralogous sequences.

The term “paralogous” relates to gene-duplications within the genome of a species leading to paralogous genes. The term “orthologous” relates to homologous genes in different organisms due to ancestral relationship. Thus, orthologs are evolutionary counterparts derived from a single ancestral gene in the last common ancestor of given two species (Koonin E V and Galperin M Y (Sequence—Evolution—Function: Computational Approaches in Comparative Genomics. Boston: Kluwer Academic; 2003. Chapter 2, Evolutionary Concept in Genetics and Genomics. Available from: ncbi.nlm.nih.gov/books/NBK20255) and therefore have great likelihood of having the same function.

One option to identify orthologues in monocot plant species is by performing a reciprocal blast search. This may be done by a first blast involving blasting the sequence-of-interest against any sequence database, such as the publicly available NCBI database which may be found at: ncbi (dot) nlm (dot) nih (dot) gov. If orthologues in rice were sought, the sequence-of-interest would be blasted against, for example, the 28,469 full-length cDNA clones from Oryza sativa Nipponbare available at NCBI. The blast results may be filtered. The full-length sequences of either the filtered results or the non-filtered results are then blasted back (second blast) against the sequences of the organism from which the sequence-of-interest is derived. The results of the first and second blasts are then compared. An orthologue is identified when the sequence resulting in the highest score (best hit) in the first blast identifies in the second blast the query sequence (the original sequence-of-interest) as the best hit. Using the same rational a paralogue (homolog to a gene in the same organism) is found. In case of large sequence families, the ClustalW program may be used [Hypertext Transfer Protocol://World Wide Web (dot) ebi (dot) ac (dot) uk/Tools/clustalw2/index (dot) html], followed by a neighbor joining tree (Hypertext Transfer Protocol://en (dot) wikipedia (dot) org/wiki/Neighbor-joining) which helps visualizing the clustering.

Homology (e.g., percent homology, identity+similarity) can be determined using any homology comparison software computing a pairwise sequence alignment.

Identity (e.g., percent homology) can be determined using any homology comparison software, including for example, the BlastN software of the National Center of Biotechnology Information (NCBI) such as by using default parameters.

According to some embodiments of the invention, the identity is a global identity, i.e., an identity over the entire amino acid or nucleic acid sequences of the invention and not over portions thereof.

According to some embodiments of the invention, the term “homology” or “homologous” refers to identity of two or more nucleic acid sequences; or identity of two or more amino acid sequences; or the identity of an amino acid sequence to one or more nucleic acid sequence.

According to some embodiments of the invention, the homology is a global homology, i.e., an homology over the entire amino acid or nucleic acid sequences of the invention and not over portions thereof.

The degree of homology or identity between two or more sequences can be determined using various known sequence comparison tools. Following is a non-limiting description of such tools which can be used along with some embodiments of the invention.

Pairwise global alignment was defined by S. B. Needleman and C. D. Wunsch, “A general method applicable to the search of similarities in the amino acid sequence of two proteins” Journal of Molecular Biology, 1970, pages 443-53, volume 48).

For example, when starting from a polypeptide sequence and comparing to other polypeptide sequences, the EMBOSS-6.0.1 Needleman-Wunsch algorithm (available from emboss(dot)sourceforge(dot)net/apps/cvs/emboss/apps/needle(dot)html) can be used to find the optimum alignment (including gaps) of two sequences along their entire length—a “Global alignment”. Default parameters for Needleman-Wunsch algorithm (EMBOSS-6.0.1) include: gapopen=10; gapextend=0.5; datafile=EBLOSUM62; brief=YES.

According to some embodiments of the invention, the parameters used with the EMBOSS-6.0.1 tool (for protein-protein comparison) include: gapopen=8; gapextend=2; datafile=EBLOSUM62; brief=YES.

According to some embodiments of the invention, the threshold used to determine homology using the EMBOSS-6.0.1 Needleman-Wunsch algorithm is 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%.

When starting from a polypeptide sequence and comparing to polynucleotide sequences, the OneModel FramePlus algorithm [Halperin, E., Faigler, S. and Gill-More, R. (1999)—FramePlus: aligning DNA to protein sequences. Bioinformatics, 15, 867-873) (available from http://biocceleration(dot)com/Products(dot)html] can be used with following default parameters: model=frametp2n.model mode=local.

According to some embodiments of the invention, the parameters used with the OneModel FramePlus algorithm are model=frametp2n.model, mode=qglobal.

According to some embodiments of the invention, the threshold used to determine homology using the OneModel FramePlus algorithm is 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%.

When starting with a polynucleotide sequence and comparing to other polynucleotide sequences the EMBOSS-6.0.1 Needleman-Wunsch algorithm (available from http://emboss(dot)sourceforge(dot)net/apps/cvs/emboss/apps/needle(dot)html) can be used with the following default parameters: (EMBOSS-6.0.1) gapopen=10; gapextend=0.5; datafile=EDNAFULL; brief=YES.

According to some embodiments of the invention, the parameters used with the EMBOSS-6.0.1 Needleman-Wunsch algorithm are gapopen=10; gapextend=0.2; datafile=EDNAFULL; brief=YES.

According to some embodiments of the invention, the threshold used to determine homology using the EMBOSS-6.0.1 Needleman-Wunsch algorithm for comparison of polynucleotides with polynucleotides is 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%.

According to some embodiment, determination of the degree of homology further requires employing the Smith-Waterman algorithm (for protein-protein comparison or nucleotide-nucleotide comparison).

Default parameters for GenCore 6.0 Smith-Waterman algorithm include: model=sw.model.

According to some embodiments of the invention, the threshold used to determine homology using the Smith-Waterman algorithm is 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%.

According to some embodiments of the invention, the global homology is performed on sequences which are pre-selected by local homology to the polypeptide or polynucleotide of interest (e.g., 60% identity over 60% of the sequence length), prior to performing the global homology to the polypeptide or polynucleotide of interest (e.g., 80% global homology on the entire sequence). For example, homologous sequences are selected using the BLAST software with the Blastp and tBlastn algorithms as filters for the first stage, and the needle (EMBOSS package) or Frame+ algorithm alignment for the second stage. Local identity (Blast alignments) is defined with a very permissive cutoff—60% Identity on a span of 60% of the sequences lengths because it is used only as a filter for the global alignment stage. In this specific embodiment (when the local identity is used), the default filtering of the Blast package is not utilized (by setting the parameter “-F F”).

In the second stage, homologs are defined based on a global identity of at least 80% to the core gene polypeptide sequence.

According to some embodiments of the invention, two distinct forms for finding the optimal global alignment for protein or nucleotide sequences are used:

1. Between two proteins (following the blastp filter):

EMBOSS-6.0.1 Needleman-Wunsch algorithm with the following modified parameters: gapopen=8 gapextend=2. The rest of the parameters are unchanged from the default options listed here:

Standard (Mandatory) qualifiers:

[-asequence]
sequence
Sequence filename and optional format, or

reference (input USA)

[-bsequence]
seqall
Sequence(s) filename and optional format,

or reference (input USA)

-gapopen float [10.0 for any sequence]. The gap open penalty is the score taken away when a gap is created. The best value depends on the choice of comparison matrix. The default value assumes you are using the EBLOSUM62 matrix for protein sequences, and the EDNAFULL matrix for nucleotide sequences. (Floating point number from 1.0 to 100.0)

-gapextend float [0.5 for any sequence]. The gap extension, penalty is added to the standard gap penalty for each base or residue in the gap. This is how long gaps are penalized. Usually you will expect a few long gaps rather than many short gaps, so the gap extension penalty should be lower than the gap penalty. An exception is where one or both sequences are single reads with possible sequencing errors in which case you would expect many single base gaps. You can get this result by setting the gap open penalty to zero (or very low) and using the gap extension penalty to control gap scoring. (Floating point number from 0.0 to 10.0)

[-outfile]
align
[*.needle] Output alignment file name

Additional (Optional) qualifiers:

- datafile matrixf [EBLOSUM62 for protein, EDNAFULL for DNA]. This is the scoring matrix file used when comparing sequences. By default it is the file ‘EBLOSUM62’ (for proteins) or the file ‘EDNAFULL’ (for nucleic sequences). These files are found in the ‘data’ directory of the EMBOSS installation.

Advanced (Unprompted) qualifiers:

-[no]brief
boolean
[Y] Brief identity and similarity

Associated Qualifiers:

“-asequence” associated qualifiers

-sbegin1
integer
Start of the sequence to be used

-send1
integer
End of the sequence to be used

-sreverse1
boolean
Reverse (if DNA)

-sask1
boolean
Ask for begin/end/reverse

-snucleotide1
boolean
Sequence is nucleotide

-sprotein1
boolean
Sequence is protein

-slower1
boolean
Make lower case

-supper1
boolean
Make upper case

-sformat1
string
Input sequence format

-sdbname1
string
Database name

-sid1
string
Entryname

-ufo1
string
UFO features

-fformat1
string
Features format

-fopenfile1
string
Features file name

“-bsequence” associated qualifiers

-sbegin2
integer
Start of each sequence to be used

-send2
integer
End of each sequence to be used

-sreverse2
boolean
Reverse (if DNA)

-sask2
boolean
Ask for begin/end/reverse

-snucleotide2
boolean
Sequence is nucleotide

-sprotein2
boolean
Sequence is protein

-slower2
boolean
Make lower case

-supper2
boolean
Make upper case

-sformat2
string
Input sequence format

-sdbname2
string
Database name

-sid2
string
Entryname

-ufo2
string
UFO features

-fformat2
string
Features format

-fopenfile2
string
Features file name

“-outfile” associated qualifiers

-aformat3
string
Alignment format

-aextension3
string
File name extension

-adirectory3
string
Output directory

-aname3
string
Base file name

-awidth3
integer
Alignment width

-aaccshow3
boolean
Show accession number in the header

-adesshow3
boolean
Show description in the header

-ausashow3
boolean
Show the full USA in the alignment

-aglobal3
boolean
Show the full sequence in alignment

General Qualifiers:

-auto
boolean
Turn off prompts

-stdout
boolean
Write first file to standard output

-filter
boolean
Read first file from standard input, write

first file to standard output

-options
boolean
Prompt for standard and additional values

-debug
boolean
Write debug output to program.dbg

-verbose
boolean
Report some/full command line options

-help
boolean
Report command line options. More information on

associated and general qualifiers can be found with

-help -verbose

-warning
boolean
Report warnings

-error
boolean
Report errors

-fatal
boolean
Report fatal errors

-die
boolean
Report dying program messages

2. Between a Protein Sequence and a Nucleotide Sequence (Following the Tblastn Filter):

GenCore 6.0 OneModel application utilizing the Frame+ algorithm with the following parameters: model=frame+_p2n.model mode=qglobal—q=protein.sequence -db=nucleotide.sequence. The rest of the parameters are unchanged from the default options:

Usage:

om -model=<model_fname> [-q=]query [-db=]database [options]

-model=<model_fname> Specifies the model that you want to run. All models supplied by Compugen are located in the directory $CGNROOT/models/.

Valid command line parameters:

-dev=<dev name> Selects the device to be used by the application.

- Valid devices are:
- bic—Bioccelerator (valid for SW, XSW, FRAME_N2P, and FRAME_P2N models).
- xlg—BioXL/G (valid for all models except XSW).
- xlp—BioXL/P (valid for SW, FRAME+N2P, and FRAME_P2N models).
- xlh—BioXL/H (valid for SW, FRAME+N2P, and FRAME_P2N models).
- soft—Software device (for all models).
  
  -q=<query> Defines the query set. The query can be a sequence file or a database reference. You can specify a query by its name or by accession number. The format is detected automatically. However, you may specify a format using the -qfmt parameter. If you do not specify a query, the program prompts for one. If the query set is a database reference, an output file is produced for each sequence in the query.
  
  -db=<database name> Chooses the database set. The database set can be a sequence file or a database reference. The database format is detected automatically. However, you may specify a format using -dfmt parameter.
  
  -qacc Add this parameter to the command line if you specify query using accession numbers.
  
  -dacc Add this parameter to the command line if you specify a database using accession numbers.
  
  -dfmt/-qfmt=<format_type> Chooses the database/query format type. Possible formats are:
- fasta—fasta with seq type auto-detected.
- fastap—fasta protein seq.
- fastan—fasta nucleic seq.
- gcg—gcg format, type is auto-detected.
- gcg9seq—gcg9 format, type is auto-detected.
- gcg9seqp—gcg9 format protein seq.
- gcg9seqn—gcg9 format nucleic seq.
- nbrf—nbrf seq, type is auto-detected.
- nbrfp—nbrf protein seq.
- nbrfn—nbrf nucleic seq.
- embl—embl and swissprot format.
- genbank—genbank format (nucleic).
- blast—blast format.
- nbrf_gcg—nbrf-gcg seq, type is auto-detected.
- nbrf_gcgp—nbrf-gcg protein seq.
- nbrf_gcgn—nbrf-gcg nucleic seq.
- raw—raw ascii sequence, type is auto-detected.
- rawp—raw ascii protein sequence.
- rawn—raw ascii nucleic sequence.
- pir—pir codata format, type is auto-detected.
- profile—gcg profile (valid only for -qfmt
- in SW, XSW, FRAME_P2N, and FRAME+_P2N).
  
  -out=<out_fname> The name of the output file.
  
  -suffix=<name> The output file name suffix.
  
  -gapop=<n> Gap open penalty. This parameter is not valid for FRAME+. For FrameSearch the default is 12.0. For other searches the default is 10.0.
  
  -gapext=<n> Gap extend penalty. This parameter is not valid for FRAME+. For FrameSearch the default is 4.0. For other models: the default for protein searches is 0.05, and the default for nucleic searches is 1.0.
  
  -qgapop=<n> The penalty for opening a gap in the query sequence. The default is 10.0. Valid for XSW.
  
  -qgapext=<n> The penalty for extending a gap in the query sequence. The default is 0.05. Valid for XSW.
  
  -start=<n> The position in the query sequence to begin the search.
  
  -end=<n> The position in the query sequence to stop the search.
  
  -qtrans Performs a translated search, relevant for a nucleic query against a protein database. The nucleic query is translated to six reading frames and a result is given for each frame.

Valid for SW and XSW.

-dtrans Performs a translated search, relevant for a protein query against a DNA database. Each database entry is translated to six reading frames and a result is given for each frame.

Valid for SW and XSW.

Note: “-qtrans” and “-dtrans” options are mutually exclusive.

-matrix=<matrix_file> Specifies the comparison matrix to be used in the search. The matrix must be in the BLAST format. If the matrix file is not located in $CGNROOT/tables/matrix, specify the full path as the value of the -matrix parameter.

-trans=<transtab_name> Translation table. The default location for the table is $ CGNROOT/tables/trans.

-onestrand Restricts the search to just the top strand of the query/database nucleic sequence.

-list=<n> The maximum size of the output hit list. The default is 50.

-docalign=<n> The number of documentation lines preceding each alignment. The default is 10.

-thr_score=<score_name> The score that places limits on the display of results. Scores that are smaller than -thr_min value or larger than -thr_max value are not shown. Valid options are: quality.

- zscore.
- escore.
  
  -thr_max=<n> The score upper threshold. Results that are larger than -thr_max value are not shown.
  
  -thr_min=<n> The score lower threshold. Results that are lower than -thr_min value are not shown.
  
  -align=<n> The number of alignments reported in the output file.
  
  -noalign Do not display alignment.
  
  Note: “-align” and “-noalign” parameters are mutually exclusive.
  
  -outfmt=<format_name> Specifies the output format type. The default format is PFS. Possible values are:
- PFS—PFS text format
- FASTA—FASTA text format
- BLAST—BLAST text format
  
  -nonorm Do not perform score normalization.
  
  -norm=<norm_name> Specifies the normalization method. Valid options are:
- log—logarithm normalization.
- std—standard normalization.
- stat—Pearson statistical method.
  
  Note: “-nonorm” and “-norm” parameters cannot be used together.
  
  Note: Parameters -xgapop, -xgapext, -fgapop, -fgapext, -ygapop, -ygapext, -delop, and -delext apply only to FRAME+.
  
  -xgapop=<n> The penalty for opening a gap when inserting a codon (triplet). The default is 12.0.
  
  -xgapext=<n> The penalty for extending a gap when inserting a codon (triplet). The default is 4.0.
  
  -ygapop=<n> The penalty for opening a gap when deleting an amino acid. The default is 12.0.
  
  -ygapext=<n> The penalty for extending a gap when deleting an amino acid. The default is 4.0.
  
  -fgapop=<n> The penalty for opening a gap when inserting a DNA base. The default is 6.0.
  
  -fgapext=<n> The penalty for extending a gap when inserting a DNA base. The default is 7.0.
  
  -delop=<n> The penalty for opening a gap when deleting a DNA base. The default is 6.0.
  
  -delext=<n> The penalty for extending a gap when deleting a DNA base. The default is 7.0.
  
  -silent No screen output is produced.
  
  -host=<host_name> The name of the host on which the server runs. By default, the application uses the host specified in the file $CGNROOT/cgnhosts.
  
  -wait Do not go to the background when the device is busy. This option is not relevant for the Parseq or Soft pseudo device.
  
  -batch Run the job in the background. When this option is specified, the file “$CGNROOT/defaults/batch.defaults” is used for choosing the batch command. If this file does not exist, the command “at now” is used to run the job.
  
  Note: “-batch” and “-wait” parameters are mutually exclusive.
  
  -version Prints the software version number.
  
  -help Displays this help message. To get more specific help type:
- “om -model=<model_fname> -help”.

According to some embodiments the homology is a local homology or a local identity.

Local alignments tools include, but are not limited to the BlastP, BlastN, BlastX or TBLASTN software of the National Center of Biotechnology Information (NCBI), FASTA, and the Smith-Waterman algorithm

A tblastn search allows the comparison between a protein sequence to the six-frame translations of a nucleotide database. It can be a very productive way of finding homologous protein coding regions in unannotated nucleotide sequences such as expressed sequence tags (ESTs) and draft genome records (HTG), located in the BLAST databases est and htgs, respectively.

Default parameters for blastp include: Max target sequences: 100; Expected threshold: e⁻⁵; Word size: 3; Max matches in a query range: 0; Scoring parameters: Matrix—BLOSUM62; filters and masking: Filter—low complexity regions.

Local alignments tools, which can be used include, but are not limited to, the tBLASTX algorithm, which compares the six-frame conceptual translation products of a nucleotide query sequence (both strands) against a protein sequence database. Default parameters include: Max target sequences: 100; Expected threshold: 10; Word size: 3; Max matches in a query range: 0; Scoring parameters: Matrix—BLOSUM62; filters and masking: Filter—low complexity regions.

According to some embodiments of the invention, the exogenous polynucleotide of the invention encodes a polypeptide having an amino acid sequence at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or more say 100% identical to the amino acid sequence selected from the group consisting of SEQ ID NOs: 475-770 and 6179-10421.

According to some embodiments of the invention, the method of increasing abiotic stress tolerance, water use efficiency, growth rate, vigor, biomass, oil content, yield, seed yield, fiber yield, fiber quality, fiber length, photosynthetic capacity, and/or fertilizer use efficiency (e.g., nitrogen use efficiency) of a plant is effected by expressing within the plant an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide at least at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or more say 100% identical to the amino acid sequence selected from the group consisting of SEQ ID NOs: 475-770 and 6179-10421, thereby increasing the abiotic stress tolerance, water use efficiency, growth rate, vigor, biomass, oil content, yield, seed yield, fiber yield, fiber quality, fiber length, photosynthetic capacity, and/or fertilizer use efficiency (e.g., nitrogen use efficiency) of the plant.

According to some embodiments of the invention, the exogenous polynucleotide encodes a polypeptide consisting of the amino acid sequence set forth by SEQ ID NO: 475-770, 6179-10420 or 10421.

According to an aspect of some embodiments of the invention, the method of increasing abiotic stress tolerance, water use efficiency, growth rate, vigor, biomass, oil content, yield, seed yield, fiber yield, fiber quality, fiber length, photosynthetic capacity, and/or fertilizer use efficiency (e.g., nitrogen use efficiency) of a plant, is effected by expressing within the plant an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 475-770 and 6179-10421, thereby increasing the abiotic stress tolerance, water use efficiency, growth rate, vigor, biomass, oil content, yield, seed yield, fiber yield, fiber quality, fiber length, photosynthetic capacity, and/or fertilizer use efficiency (e.g., nitrogen use efficiency) of the plant.

According to some embodiments of the invention, the exogenous polynucleotide encodes a polypeptide consisting of the amino acid sequence set forth by SEQ ID NO: 475-770 and 6179-10421.

According to some embodiments of the invention the exogenous polynucleotide comprises a nucleic acid sequence which is at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, e.g., 100% identical to the nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-474 and 771-6178.

According to an aspect of some embodiments of the invention, there is provided a method of increasing abiotic stress tolerance, water use efficiency, growth rate, vigor, biomass, oil content, yield, seed yield, fiber yield, fiber quality, fiber length, photosynthetic capacity, and/or fertilizer use efficiency (e.g., nitrogen use efficiency) of a plant, comprising expressing within the plant an exogenous polynucleotide comprising a nucleic acid sequence at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, e.g., 100% identical to the nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-474 and 771-6178, thereby increasing the abiotic stress tolerance, water use efficiency, growth rate, vigor, biomass, oil content, yield, seed yield, fiber yield, fiber quality, fiber length, photosynthetic capacity, and/or fertilizer use efficiency (e.g., nitrogen use efficiency) of the plant.

According to some embodiments of the invention the exogenous polynucleotide is at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, e.g., 100% identical to the polynucleotide selected from the group consisting of SEQ ID NOs: 1-474 and 771-6178.

According to some embodiments of the invention the exogenous polynucleotide is set forth by SEQ ID NO: 1-474, 771-6177 or 6178.

As used herein the term “polynucleotide” refers to a single or double stranded nucleic acid sequence which is isolated and provided in the form of an RNA sequence, a complementary polynucleotide sequence (cDNA), a genomic polynucleotide sequence and/or a composite polynucleotide sequences (e.g., a combination of the above).

The term “isolated” refers to at least partially separated from the natural environment e.g., from a plant cell.

As used herein the phrase “complementary polynucleotide sequence” refers to a sequence, which results from reverse transcription of messenger RNA using a reverse transcriptase or any other RNA dependent DNA polymerase. Such a sequence can be subsequently amplified in vivo or in vitro using a DNA dependent DNA polymerase.

As used herein the phrase “genomic polynucleotide sequence” refers to a sequence derived (isolated) from a chromosome and thus it represents a contiguous portion of a chromosome.

As used herein the phrase “composite polynucleotide sequence” refers to a sequence, which is at least partially complementary and at least partially genomic. A composite sequence can include some exonal sequences required to encode the polypeptide of the present invention, as well as some intronic sequences interposing therebetween. The intronic sequences can be of any source, including of other genes, and typically will include conserved splicing signal sequences. Such intronic sequences may further include cis acting expression regulatory elements.

Nucleic acid sequences encoding the polypeptides of the present invention may be optimized for expression. Examples of such sequence modifications include, but are not limited to, an altered G/C content to more closely approach that typically found in the plant species of interest, and the removal of codons atypically found in the plant species commonly referred to as codon optimization.

The phrase “codon optimization” refers to the selection of appropriate DNA nucleotides for use within a structural gene or fragment thereof that approaches codon usage within the plant of interest. Therefore, an optimized gene or nucleic acid sequence refers to a gene in which the nucleotide sequence of a native or naturally occurring gene has been modified in order to utilize statistically-preferred or statistically-favored codons within the plant. The nucleotide sequence typically is examined at the DNA level and the coding region optimized for expression in the plant species determined using any suitable procedure, for example as described in Sardana et al. (1996, Plant Cell Reports 15:677-681). In this method, the standard deviation of codon usage, a measure of codon usage bias, may be calculated by first finding the squared proportional deviation of usage of each codon of the native gene relative to that of highly expressed plant genes, followed by a calculation of the average squared deviation. The formula used is: 1 SDCU=n=1 N [(Xn−Yn)/Yn]2/N, where Xn refers to the frequency of usage of codon n in highly expressed plant genes, where Yn to the frequency of usage of codon n in the gene of interest and N refers to the total number of codons in the gene of interest. A Table of codon usage from highly expressed genes of dicotyledonous plants is compiled using the data of Murray et al. (1989, Nuc Acids Res. 17:477-498).

One method of optimizing the nucleic acid sequence in accordance with the preferred codon usage for a particular plant cell type is based on the direct use, without performing any extra statistical calculations, of codon optimization Tables such as those provided on-line at the Codon Usage Database through the NIAS (National Institute of Agrobiological Sciences) DNA bank in Japan (Hypertext Transfer Protocol://World Wide Web (dot) kazusa (dot) or (dot) jp/codon/). The Codon Usage Database contains codon usage tables for a number of different species, with each codon usage Table having been statistically determined based on the data present in Genbank.

By using the above Tables to determine the most preferred or most favored codons for each amino acid in a particular species (for example, rice), a naturally-occurring nucleotide sequence encoding a protein of interest can be codon optimized for that particular plant species. This is effected by replacing codons that may have a low statistical incidence in the particular species genome with corresponding codons, in regard to an amino acid, that are statistically more favored. However, one or more less-favored codons may be selected to delete existing restriction sites, to create new ones at potentially useful junctions (5′ and 3′ ends to add signal peptide or termination cassettes, internal sites that might be used to cut and splice segments together to produce a correct full-length sequence), or to eliminate nucleotide sequences that may negatively effect mRNA stability or expression.

The naturally-occurring encoding nucleotide sequence may already, in advance of any modification, contain a number of codons that correspond to a statistically-favored codon in a particular plant species. Therefore, codon optimization of the native nucleotide sequence may comprise determining which codons, within the native nucleotide sequence, are not statistically-favored with regards to a particular plant, and modifying these codons in accordance with a codon usage table of the particular plant to produce a codon optimized derivative. A modified nucleotide sequence may be fully or partially optimized for plant codon usage provided that the protein encoded by the modified nucleotide sequence is produced at a level higher than the protein encoded by the corresponding naturally occurring or native gene. Construction of synthetic genes by altering the codon usage is described in for example WO publication 93/07278 (Koziel M., et al.).

According to some embodiments of the invention, the exogenous polynucleotide is a non-coding RNA.

As used herein the phrase “non-coding RNA” refers to an RNA molecule which does not encode an amino acid sequence (a polypeptide). Examples of such non-coding RNA molecules include, but are not limited to, an antisense RNA, a pre-miRNA (precursor of a microRNA), or a precursor of a Piwi-interacting RNA (piRNA).

Non-limiting examples of non-coding RNA polynucleotides are provided in SEQ ID NOs: 217, 218, 261, 262, 473, 474, 2066, 2827, 2886, 3165, 3166, 3167, 3168, 3180, 3181, 3213, 3215, 5247, 6108, 6163.

Thus, the invention encompasses nucleic acid sequences described hereinabove; fragments thereof, sequences hybridizable therewith, sequences homologous thereto, sequences encoding similar polypeptides with different codon usage, altered sequences characterized by mutations, such as deletion, insertion or substitution of one or more nucleotides, either naturally occurring or man induced, either randomly or in a targeted fashion.

The invention provides an isolated polynucleotide comprising a nucleic acid sequence at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, e.g., 100% identical to the polynucleotide selected from the group consisting of SEQ ID NOs: 1-474 and 771-6178.

According to some embodiments of the invention the nucleic acid sequence is capable of increasing abiotic stress tolerance, water use efficiency, growth rate, vigor, biomass, oil content, yield, seed yield, fiber yield, fiber quality, fiber length, photosynthetic capacity, and/or fertilizer use efficiency (e.g., nitrogen use efficiency) of a plant.

According to some embodiments of the invention the isolated polynucleotide comprising the nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-474 and 771-6178.

According to some embodiments of the invention the isolated polynucleotide is set forth by SEQ ID NO: 1-474, 771-6177 or 6178.

The invention provides an isolated polynucleotide comprising a nucleic acid sequence encoding a polypeptide which comprises an amino acid sequence at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or more say 100% homologous to the amino acid sequence selected from the group consisting of SEQ ID NO: 475-770, 6179-10420 or 10421.

According to some embodiments of the invention the amino acid sequence is capable of increasing abiotic stress tolerance, water use efficiency, growth rate, vigor, biomass, oil content, yield, seed yield, fiber yield, fiber quality, fiber length, photosynthetic capacity, and/or fertilizer use efficiency (e.g., nitrogen use efficiency) of a plant.

The invention provides an isolated polynucleotide comprising a nucleic acid sequence encoding a polypeptide which comprises the amino acid sequence selected from the group consisting of SEQ ID NOs: 475-770 and 6179-10421.

According to an aspect of some embodiments of the invention, there is provided a nucleic acid construct comprising the isolated polynucleotide of the invention, and a promoter for directing transcription of the nucleic acid sequence in a host cell.

The invention provides an isolated polypeptide comprising an amino acid sequence at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or more say 100% homologous to an amino acid sequence selected from the group consisting of SEQ ID NO: 475-770 and 6179-10421.

According to some embodiments of the invention, the polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 475-770 and 6179-10421.

According to some embodiments of the invention, the polypeptide is set forth by SEQ ID NO: 475-770 and 6179-10421.

The invention also encompasses fragments of the above described polypeptides and polypeptides having mutations, such as deletions, insertions or substitutions of one or more amino acids, either naturally occurring or man induced, either randomly or in a targeted fashion.

The term “plant” as used herein encompasses whole plants, ancestors and progeny of the plants and plant parts, including seeds, shoots, stems, roots (including tubers), and plant cells, tissues and organs. The plant may be in any form including suspension cultures, embryos, meristematic regions, callus tissue, leaves, gametophytes, sporophytes, pollen, and microspores. Plants that are particularly useful in the methods of the invention include all plants which belong to the superfamily Viridiplantae, in particular monocotyledonous and dicotyledonous plants including a fodder or forage legume, ornamental plant, food crop, tree, or shrub selected from the list comprising Acacia spp., Acer spp., Actinidia spp., Aesculus spp., Agathis australis, Albizia amara, Alsophila tricolor, Andropogon spp., Arachis spp, Areca catechu, Astelia fragrans, Astragalus cicer, Baikiaea plurijuga, Betula spp., Brassica spp., Bruguiera gymnorrhiza , Burkea africana, Butea frondosa, Cadaba farinosa, Calliandra spp, Camellia sinensis, Canna indica, Capsicum spp., Cassia spp., Centroema pubescens, Chacoomeles spp., Cinnamomum cassia, Coffea arabica, Colophospermum mopane, Coronillia varia, Cotoneaster serotina, Crataegus spp., Cucumis spp., Cupressus spp., Cyathea dealbata, Cydonia oblonga, Cryptomeria japonica, Cymbopogon spp., Cynthea dealbata, Cydonia oblonga, Dalbergia monetaria, Davallia divaricata, Desmodium spp., Dicksonia squarosa, Dibeteropogon amplectens , Dioclea spp, Dolichos spp., Dorycnium rectum, Echinochloa pyramidalis, Ehraffia spp., Eleusine coracana, Eragrestis spp., Erythrina spp., Eucalypfus spp., Euclea schimperi, Eulalia vi/losa, Pagopyrum spp., Feijoa sellowlana, Fragaria spp., Flemingia spp, Freycinetia banksli, Geranium thunbergii, GinAgo biloba, Glycine javanica, Gliricidia spp, Gossypium hirsutum, Grevillea spp., Guibourtia coleosperma, Hedysarum spp., Hemaffhia altissima, Heteropogon contoffus, Hordeum vulgare, Hyparrhenia rufa, Hypericum erectum, Hypeffhelia dissolute, Indigo incamata, Iris spp., Leptarrhena pyrolifolia, Lespediza spp., Lettuca spp., Leucaena leucocephala, Loudetia simplex, Lotonus bainesli, Lotus spp., Macrotyloma axillare, Malus spp., Manihot esculenta, Medicago saliva, Metasequoia glyptostroboides, Musa sapientum, Nicotianum spp., Onobrychis spp., Ornithopus spp., Oryza spp., Peltophorum africanum, Pennisetum spp., Persea gratissima, Petunia spp., Phaseolus spp., Phoenix canariensis, Phormium cookianum, Photinia spp., Picea glauca, Pinus spp., Pisum sativam, Podocarpus totara, Pogonarthria fleckii, Pogonaffhria squarrosa, Populus spp., Prosopis cineraria, Pseudotsuga menziesii, Pterolobium stellatum, Pyrus communis, Quercus spp., Rhaphiolepsis umbellata, Rhopalostylis sapida, Rhus natalensis, Ribes grossularia, Ribes spp., Robinia pseudoacacia, Rosa spp., Rubus spp., Salix spp., Schyzachyrium sanguineum, Sciadopitys vefficillata, Sequoia sempervirens, Sequoiadendron giganteum, Sorghum bicolor, Spinacia spp., Sporobolus fimbriatus, Stiburus alopecuroides, Stylosanthos humilis, Tadehagi spp, Taxodium distichum, Themeda triandra, Trifolium spp., Triticum spp., Tsuga heterophylla, Vaccinium spp., Vicia spp., Vitis vinifera, Watsonia pyramidata, Zantedeschia aethiopica, Zea mays, amaranth, artichoke, asparagus, broccoli, Brussels sprouts, cabbage, canola, carrot, cauliflower, celery, collard greens, flax, kale, lentil, oilseed rape, okra, onion, potato, rice, soybean, straw, sugar beet, sugar cane, sunflower, tomato, squash tea, maize, wheat, barley, rye, oat, peanut, pea, lentil and alfalfa, cotton, rapeseed, canola, pepper, sunflower, tobacco, eggplant, eucalyptus, a tree, an ornamental plant, a perennial grass and a forage crop. Alternatively algae and other non-Viridiplantae can be used for the methods of the present invention.

According to some embodiments of the invention, the plant used by the method of the invention is a crop plant such as rice, maize, wheat, barley, peanut, potato, sesame, olive tree, palm oil, banana, soybean, sunflower, canola, sugarcane, alfalfa, millet, leguminosae (bean, pea), flax, lupinus, rapeseed, tobacco, poplar and cotton.

According to some embodiments of the invention the plant is a dicotyledonous plant.

According to some embodiments of the invention the plant is a monocotyledonous plant.

According to some embodiments of the invention, there is provided a plant cell exogenously expressing the polynucleotide of some embodiments of the invention, the nucleic acid construct of some embodiments of the invention and/or the polypeptide of some embodiments of the invention.

According to some embodiments of the invention, expressing the exogenous polynucleotide of the invention within the plant is effected by transforming one or more cells of the plant with the exogenous polynucleotide, followed by generating a mature plant from the transformed cells and cultivating the mature plant under conditions suitable for expressing the exogenous polynucleotide within the mature plant.

According to some embodiments of the invention, the transformation is effected by introducing to the plant cell a nucleic acid construct which includes the exogenous polynucleotide of some embodiments of the invention and at least one promoter for directing transcription of the exogenous polynucleotide in a host cell (a plant cell). Further details of suitable transformation approaches are provided hereinbelow.

As mentioned, the nucleic acid construct according to some embodiments of the invention comprises a promoter sequence and the isolated polynucleotide of the some embodiments of invention.

According to some embodiments of the invention, the isolated polynucleotide is operably linked to the promoter sequence.

A coding nucleic acid sequence is “operably linked” to a regulatory sequence (e.g., promoter) if the regulatory sequence is capable of exerting a regulatory effect on the coding sequence linked thereto.

As used herein, the term “promoter” refers to a region of DNA which lies upstream of the transcriptional initiation site of a gene to which RNA polymerase binds to initiate transcription of RNA. The promoter controls where (e.g., which portion of a plant) and/or when (e.g., at which stage or condition in the lifetime of an organism) the gene is expressed.

According to some embodiments of the invention, the promoter is heterologous to the isolated polynucleotide and/or to the host cell.

As used herein the phrase “heterologous promoter” refers to a promoter from a different species or from the same species but from a different gene locus as of the isolated polynucleotide sequence.

Any suitable promoter sequence can be used by the nucleic acid construct of the present invention. Preferably the promoter is a constitutive promoter, a tissue-specific, or an abiotic stress-inducible promoter.

According to some embodiments of the invention, the promoter is a plant promoter, which is suitable for expression of the exogenous polynucleotide in a plant cell.

Suitable promoters for expression in wheat include, but are not limited to, Wheat SPA promoter (SEQ ID NO: 10422; Albanietal, Plant Cell, 9: 171-184, 1997, which is fully incorporated herein by reference), wheat LMW (SEQ ID NO: 10423 (longer LMW promoter), and SEQ ID NO: 10424 (LMW promoter) and HMW glutenin-1 (SEQ ID NO: 10425 (Wheat HMW glutenin-1 longer promoter); and SEQ ID NO: 10426 (Wheat HMW glutenin-1 Promoter); Thomas and Flavell, The Plant Cell 2:1171-1180; Furtado et al., 2009 Plant Biotechnology Journal 7:240-253, each of which is fully incorporated herein by reference), wheat alpha, beta and gamma gliadins [e.g., SEQ ID NO: 10427 (wheat alpha gliadin, B genome promoter); SEQ ID NO: 10428 (wheat gamma gliadin promoter); EMBO 3:1409-15, 1984, which is fully incorporated herein by reference], wheat TdPR60 [SEQ ID NO:10429 (wheat TdPR60 longer promoter) or SEQ ID NO:10430 (wheat TdPR60 promoter); Kovalchuk et al., Plant Mol Biol 71:81-98, 2009, which is fully incorporated herein by reference], maize Ub1 Promoter [cultivar Nongda 105 (SEQ ID NO:10431); GenBank: DQ141598.1; Taylor et al., Plant Cell Rep 1993 12: 491-495, which is fully incorporated herein by reference; and cultivar B73 (SEQ ID NO:10432); Christensen, A H, et al. Plant Mol. Biol. 18 (4), 675-689 (1992), which is fully incorporated herein by reference]; rice actin 1 (SEQ ID NO:10433; Mc Elroy et al. 1990, The Plant Cell, Vol. 2, 163-171, which is fully incorporated herein by reference), rice GOS2 [SEQ ID NO: 10434 (rice GOS2 longer promoter) and SEQ ID NO: 10435 (rice GOS2 Promoter); De Pater et al. Plant J. 1992; 2: 837-44, which is fully incorporated herein by reference], arabidopsis Phol [SEQ ID NO: 10436 (arabidopsis Pho1 Promoter); Hamburger et al., Plant Cell. 2002; 14: 889-902, which is fully incorporated herein by reference], ExpansinB promoters, e.g., rice ExpB5 [SEQ ID NO:10437 (rice ExpB5 longer promoter) and SEQ ID NO: 10438 (rice ExpB5 promoter)] and Barley ExpB1 [SEQ ID NO: 10439 (barley ExpB1 Promoter), Won et al. Mol Cells. 2010; 30:369-76, which is fully incorporated herein by reference], barley SS2 (sucrose synthase 2) [(SEQ ID NO: 10440), Guerin and Carbonero, Plant Physiology May 1997 vol. 114 no. 1 55-62, which is fully incorporated herein by reference], and rice PG5a [SEQ ID NO:10441, U.S. Pat. No. 7,700,835, Nakase et al., Plant Mol Biol. 32:621-30, 1996, each of which is fully incorporated herein by reference].

Suitable constitutive promoters include, for example, CaMV 35S promoter [SEQ ID NO: 10442 (CaMV 35S (QFNC) Promoter); SEQ ID NO: 10443 (PJJ 35S from Brachypodium); SEQ ID NO: 10444 (CaMV 35S (OLD) Promoter) (Odell et al., Nature 313:810-812, 1985)], Arabidopsis At6669 promoter (SEQ ID NO: 10445 (Arabidopsis At6669 (OLD) Promoter); see PCT Publication No. W004081173A2 or the new At6669 promoter (SEQ ID NO: 10446 (Arabidopsis At6669 (NEW) Promoter)); maize Ub1 Promoter [cultivar Nongda 105 (SEQ ID NO:10431); GenBank: DQ141598.1; Taylor et al., Plant Cell Rep 1993 12: 491-495, which is fully incorporated herein by reference; and cultivar B73 (SEQ ID NO:10432); Christensen, A H, et al. Plant Mol. Biol. 18 (4), 675-689 (1992), which is fully incorporated herein by reference]; rice actin 1 (SEQ ID NO: 10433, McElroy et al., Plant Cell 2:163-171, 1990); pEMU (Last et al., Theor. Appl. Genet. 81:581-588, 1991); CaMV 19S (Nilsson et al., Physiol. Plant 100:456-462, 1997); rice GOS2 [SEQ ID NO: 10434 (rice GOS2 longer Promoter) and SEQ ID NO: 10435 (rice GOS2 Promoter), de Pater et al, Plant J November; 2(6):837-44, 1992]; RBCS promoter (SEQ ID NO:10447); Rice cyclophilin (Bucholz et al, Plant Mol Biol. 25(5):837-43, 1994); Maize H3 histone (Lepetit et al, Mol. Gen. Genet. 231: 276-285, 1992); Actin 2 (An et al, Plant J. 10(1);107-121, 1996) and Synthetic Super MAS (Ni et al., The Plant Journal 7: 661-76, 1995). Other constitutive promoters include those in U.S. Pat. Nos. 5,659,026, 5,608,149; 5,608,144; 5,604,121; 5,569,597: 5,466,785; 5,399,680; 5,268,463; and 5,608,142.

Suitable tissue-specific promoters include, but not limited to, leaf-specific promoters [e.g., AT5G06690 (Thioredoxin) (high expression, SEQ ID NO: 10448), AT5G61520 (AtSTP3) (low expression, SEQ ID NO: 10449) described in Buttner et al 2000 Plant, Cell and Environment 23, 175-184, or the promoters described in Yamamoto et al., Plant J. 12:255-265, 1997; Kwon et al., Plant Physiol. 105:357-67, 1994; Yamamoto et al., Plant Cell Physiol. 35:773-778, 1994; Gotor et al., Plant J. 3:509-18, 1993; Orozco et al., Plant Mol. Biol. 23:1129-1138, 1993; and Matsuoka et al., Proc. Natl. Acad. Sci. USA 90:9586-9590, 1993; as well as Arabidopsis STP3 (AT5G61520) promoter (Buttner et al., Plant, Cell and Environment 23:175-184, 2000)], seed-preferred promoters [e.g., Napin (originated from Brassica napus which is characterized by a seed specific promoter activity; Stuitje A. R. et. al. Plant Biotechnology Journal 1 (4): 301-309; SEQ ID NO: 10450 (Brassica napus NAPIN Promoter) from seed specific genes (Simon, et al., Plant Mol. Biol. 5. 191, 1985; Scofield, et al., J. Biol. Chem. 262: 12202, 1987; Baszczynski, et al., Plant Mol. Biol. 14: 633, 1990), rice PG5a (SEQ ID NO: 10441; U.S. Pat. No. 7,700,835), early seed development Arabidopsis BAN (AT1G61720) (SEQ ID NO: 10451, US 2009/0031450 A1), late seed development Arabidopsis ABI3 (AT3G24650) (SEQ ID NO: 10452, Arabidopsis ABI3 (AT3G24650) longer Promoter) or SEQ ID NO: 10453 (Arabidopsis ABI3 (AT3G24650) Promoter)) (Ng et al., Plant Molecular Biology 54: 25-38, 2004), Brazil Nut albumin (Pearson′ et al., Plant Mol. Biol. 18: 235-245, 1992), legumin (Ellis, et al. Plant Mol. Biol. 10: 203-214, 1988), Glutelin (rice) (Takaiwa, et al., Mol. Gen. Genet. 208: 15-22, 1986; Takaiwa, et al., FEBS Letts. 221: 43-47, 1987), Zein (Matzke et al Plant Mol Biol, 143).323-32 1990), napA (Stalberg, et al, Planta 199: 515-519, 1996), Wheat SPA (SEQ ID NO:10422; Albanietal, Plant Cell, 9: 171-184, 1997), sunflower oleosin (Cummins, et al., Plant Mol. Biol. 19: 873-876, 1992)], endosperm specific promoters [e.g., wheat LMW (SEQ ID NO: 10423 (Wheat LMW Longer Promoter), and SEQ ID NO: 10424 (Wheat LMW Promoter) and HMW glutenin-1 [(SEQ ID NO: 10425 (Wheat HMW glutenin-1 longer Promoter)); and SEQ ID NO: 10426 (Wheat HMW glutenin-1 Promoter), Thomas and Flavell, The Plant Cell 2:1171-1180, 1990; Mol Gen Genet 216:81-90, 1989; NAR 17:461-2), wheat alpha, beta and gamma gliadins (SEQ ID NO: 10427 (wheat alpha gliadin (B genome) promoter); SEQ ID NO: 10428 (wheat gamma gliadin promoter); EMBO 3:1409-15, 1984), Barley ltrl promoter, barley Bl, C, D hordein (Theor Appl Gen 98:1253-62, 1999; Plant J 4:343-55, 1993; Mol Gen Genet 250:750-60, 1996), Barley DOF (Mena et al, The Plant Journal, 116(1): 53-62, 1998), Biz2 (EP99106056.7), Barley SS2 (SEQ ID NO: 10440 (Barley SS2 Promoter); Guerin and Carbonero Plant Physiology 114: 1 55-62, 1997), wheat Tarp60 (Kovalchuk et al., Plant Mol Biol 71:81-98, 2009), barley D-hordein (D-Hor) and B-hordein (B-Hor) (Agnelo Furtado, Robert J. Henry and Alessandro Pellegrineschi (2009)], Synthetic promoter (Vicente-Carbajosa et al., Plant J. 13: 629-640, 1998), rice prolamin NRP33, rice -globulin Glb-1 (Wu et al, Plant Cell Physiology 39(8) 885-889, 1998), rice alpha-globulin REB/OHP-1 (Nakase et al. Plant Mol. Biol. 33: 513-S22, 1997), rice ADP-glucose PP (Trans Res 6:157-68, 1997), maize ESR gene family (Plant J 12:235-46, 1997), sorgum gamma-kafirin (PMB 32:1029-35, 1996)], embryo specific promoters [e.g., rice OSH1 (Sato et al, Proc. Natl. Acad. Sci. USA, 93: 8117-8122), KNOX (Postma-Haarsma et al, Plant Mol. Biol. 39:257-71, 1999), rice oleosin (Wu et at, J. Biochem., 123:386, 1998)], and flower-specific promoters [e.g., AtPRP4, chalene synthase (chsA) (Van der Meer, et al., Plant Mol. Biol. 15, 95-109, 1990), LAT52 (Twell et al Mol. Gen Genet. 217:240-245; 1989), Arabidopsis apetala—3 (Tilly et al., Development. 125:1647-57, 1998), Arabidopsis APETALA 1 (AT1G69120, AP1) (SEQ ID NO: 10454 (Arabidopsis (AT1G69120) APETALA 1)) (Hempel et al., Development 124:3845-3853, 1997)], and root promoters [e.g., the ROOTP promoter [SEQ ID NO: 10455]; rice ExpB5 (SEQ ID NO:10438 (rice ExpB5 Promoter); or SEQ ID NO: 10437 (rice ExpB5 longer Promoter)) and barley ExpB1 promoters (SEQ ID NO:10439) (Won et al. Mol. Cells 30: 369-376, 2010); arabidopsis ATTPS-CIN (AT3G25820) promoter (SEQ ID NO: 10456; Chen et al., Plant Phys 135:1956-66, 2004); Arabidopsis Pho1 promoter (SEQ ID NO: 10436, Hamburger et al., Plant Cell. 14: 889-902, 2002), which is also slightly induced by stress].

Suitable abiotic stress-inducible promoters include, but not limited to, salt-inducible promoters such as RD29A (Yamaguchi-Shinozalei et al., Mol. Gen. Genet. 236:331-340, 1993); drought-inducible promoters such as maize rabl7 gene promoter (Pla et. al., Plant Mol. Biol. 21:259-266, 1993), maize rab28 gene promoter (Busk et. al., Plant J. 11:1285-1295, 1997) and maize Ivr2 gene promoter (Pelleschi et. al., Plant Mol. Biol. 39:373-380, 1999); heat-inducible promoters such as heat tomato hsp80-promoter from tomato (U.S. Pat. No. 5,187,267).

The nucleic acid construct of some embodiments of the invention can further include an appropriate selectable marker and/or an origin of replication. According to some embodiments of the invention, the nucleic acid construct utilized is a shuttle vector, which can propagate both in E. coli (wherein the construct comprises an appropriate selectable marker and origin of replication) and be compatible with propagation in cells. The construct according to the present invention can be, for example, a plasmid, a bacmid, a phagemid, a cosmid, a phage, a virus or an artificial chromosome.

The nucleic acid construct of some embodiments of the invention can be utilized to stably or transiently transform plant cells. In stable transformation, the exogenous polynucleotide is integrated into the plant genome and as such it represents a stable and inherited trait. In transient transformation, the exogenous polynucleotide is expressed by the cell transformed but it is not integrated into the genome and as such it represents a transient trait.

There are various methods of introducing foreign genes into both monocotyledonous and dicotyledonous plants (Potrykus, I., Annu. Rev. Plant. Physiol., Plant. Mol. Biol. (1991) 42:205-225; Shimamoto et al., Nature (1989) 338:274-276).

The principle methods of causing stable integration of exogenous DNA into plant genomic DNA include two main approaches:

(i) Agrobacterium-mediated gene transfer: Klee et al. (1987) Annu. Rev. Plant Physiol. 38:467-486; Klee and Rogers in Cell Culture and Somatic Cell Genetics of Plants, Vol. 6, Molecular Biology of Plant Nuclear Genes, eds. Schell, J., and Vasil, L. K., Academic Publishers, San Diego, Calif. (1989) p. 2-25; Gatenby, in Plant Biotechnology, eds. Kung, S. and Arntzen, C. J., Butterworth Publishers, Boston, Mass. (1989) p. 93-112.

(ii) Direct DNA uptake: Paszkowski et al., in Cell Culture and Somatic Cell Genetics of Plants, Vol. 6, Molecular Biology of Plant Nuclear Genes eds. Schell, J., and Vasil, L. K., Academic Publishers, San Diego, Calif. (1989) p. 52-68; including methods for direct uptake of DNA into protoplasts, Toriyama, K. et al. (1988) Bio/Technology 6:1072-1074. DNA uptake induced by brief electric shock of plant cells: Zhang et al. Plant Cell Rep. (1988) 7:379-384. Fromm et al. Nature (1986) 319:791-793. DNA injection into plant cells or tissues by particle bombardment, Klein et al. Bio/Technology (1988) 6:559-563; McCabe et al. Bio/Technology (1988) 6: 923 -926; Sanford, Physiol. Plant. (1990) 79:206-209; by the use of micropipette systems: Neuhaus et al., Theor. Appl. Genet. (1987) 75:30-36; Neuhaus and Spangenberg, Physiol. Plant. (1990) 79:213-217; glass fibers or silicon carbide whisker transformation of cell cultures, embryos or callus tissue, U.S. Pat. No. 5,464,765 or by the direct incubation of DNA with germinating pollen, DeWet et al. in Experimental Manipulation of Ovule Tissue, eds. Chapman, G. P. and Mantell, S. H. and Daniels, W. Longman, London, (1985) p. 197-209; and Ohta, Proc. Natl. Acad. Sci. USA (1986) 83:715-719.

The Agrobacterium system includes the use of plasmid vectors that contain defined DNA segments that integrate into the plant genomic DNA. Methods of inoculation of the plant tissue vary depending upon the plant species and the Agrobacterium delivery system. A widely used approach is the leaf disc procedure which can be performed with any tissue explant that provides a good source for initiation of whole plant differentiation. See, e.g., Horsch et al. in Plant Molecular Biology Manual A5, Kluwer Academic Publishers, Dordrecht (1988) p. 1-9. A supplementary approach employs the Agrobacterium delivery system in combination with vacuum infiltration. The Agrobacterium system is especially viable in the creation of transgenic dicotyledonous plants.

There are various methods of direct DNA transfer into plant cells. In electroporation, the protoplasts are briefly exposed to a strong electric field. In microinjection, the DNA is mechanically injected directly into the cells using very small micropipettes. In microparticle bombardment, the DNA is adsorbed on microprojectiles such as magnesium sulfate crystals or tungsten particles, and the microprojectiles are physically accelerated into cells or plant tissues.

Following stable transformation plant propagation is exercised. The most common method of plant propagation is by seed. Regeneration by seed propagation, however, has the deficiency that due to heterozygosity there is a lack of uniformity in the crop, since seeds are produced by plants according to the genetic variances governed by Mendelian rules. Basically, each seed is genetically different and each will grow with its own specific traits. Therefore, it is preferred that the transformed plant be produced such that the regenerated plant has the identical traits and characteristics of the parent transgenic plant. Therefore, it is preferred that the transformed plant be regenerated by micropropagation which provides a rapid, consistent reproduction of the transformed plants.

Micropropagation is a process of growing new generation plants from a single piece of tissue that has been excised from a selected parent plant or cultivar. This process permits the mass reproduction of plants having the preferred tissue expressing the fusion protein. The new generation plants which are produced are genetically identical to, and have all of the characteristics of, the original plant. Micropropagation allows mass production of quality plant material in a short period of time and offers a rapid multiplication of selected cultivars in the preservation of the characteristics of the original transgenic or transformed plant. The advantages of cloning plants are the speed of plant multiplication and the quality and uniformity of plants produced.

Micropropagation is a multi-stage procedure that requires alteration of culture medium or growth conditions between stages. Thus, the micropropagation process involves four basic stages: Stage one, initial tissue culturing; stage two, tissue culture multiplication; stage three, differentiation and plant formation; and stage four, greenhouse culturing and hardening. During stage one, initial tissue culturing, the tissue culture is established and certified contaminant-free. During stage two, the initial tissue culture is multiplied until a sufficient number of tissue samples are produced from the seedlings to meet production goals. During stage three, the tissue samples grown in stage two are divided and grown into individual plantlets. At stage four, the transformed plantlets are transferred to a greenhouse for hardening where the plants' tolerance to light is gradually increased so that it can be grown in the natural environment.

According to some embodiments of the invention, the transgenic plants are generated by transient transformation of leaf cells, meristematic cells or the whole plant.

Transient transformation can be effected by any of the direct DNA transfer methods described above or by viral infection using modified plant viruses.

Viruses that have been shown to be useful for the transformation of plant hosts include CaMV, Tobacco mosaic virus (TMV), brome mosaic virus (BMV) and Bean Common Mosaic Virus (BV or BCMV). Transformation of plants using plant viruses is described in U.S. Pat. No. 4,855,237 (bean golden mosaic virus; BGV), EP-A 67,553 (TMV), Japanese Published Application No. 63-14693 (TMV), EPA 194,809 (BV), EPA 278,667 (BV); and Gluzman, Y. et al., Communications in Molecular Biology: Viral Vectors, Cold Spring Harbor Laboratory, New York, pp. 172-189 (1988). Pseudovirus particles for use in expressing foreign DNA in many hosts, including plants are described in WO 87/06261.

According to some embodiments of the invention, the virus used for transient transformations is avirulent and thus is incapable of causing severe symptoms such as reduced growth rate, mosaic, ring spots, leaf roll, yellowing, streaking, pox formation, tumor formation and pitting. A suitable avirulent virus may be a naturally occurring avirulent virus or an artificially attenuated virus. Virus attenuation may be effected by using methods well known in the art including, but not limited to, sub-lethal heating, chemical treatment or by directed mutagenesis techniques such as described, for example, by Kurihara and Watanabe (Molecular Plant Pathology 4:259-269, 2003), Gal-on et al. (1992), Atreya et al. (1992) and Huet et al. (1994).

Suitable virus strains can be obtained from available sources such as, for example, the American Type culture Collection (ATCC) or by isolation from infected plants. Isolation of viruses from infected plant tissues can be effected by techniques well known in the art such as described, for example by Foster and Taylor, Eds. “Plant Virology Protocols: From Virus Isolation to Transgenic Resistance (Methods in Molecular Biology (Humana Pr), Vol 81)”, Humana Press, 1998. Briefly, tissues of an infected plant believed to contain a high concentration of a suitable virus, preferably young leaves and flower petals, are ground in a buffer solution (e.g., phosphate buffer solution) to produce a virus infected sap which can be used in subsequent inoculations.

Construction of plant RNA viruses for the introduction and expression of non-viral exogenous polynucleotide sequences in plants is demonstrated by the above references as well as by Dawson, W. O. et al., Virology (1989) 172:285-292; Takamatsu et al. EMBO J. (1987) 6:307-311; French et al. Science (1986) 231:1294-1297; Takamatsu et al. FEBS Letters (1990) 269:73-76; and U.S. Pat. No. 5,316,931.

When the virus is a DNA virus, suitable modifications can be made to the virus itself. Alternatively, the virus can first be cloned into a bacterial plasmid for ease of constructing the desired viral vector with the foreign DNA. The virus can then be excised from the plasmid. If the virus is a DNA virus, a bacterial origin of replication can be attached to the viral DNA, which is then replicated by the bacteria. Transcription and translation of this DNA will produce the coat protein which will encapsidate the viral DNA. If the virus is an RNA virus, the virus is generally cloned as a cDNA and inserted into a plasmid. The plasmid is then used to make all of the constructions. The RNA virus is then produced by transcribing the viral sequence of the plasmid and translation of the viral genes to produce the coat protein(s) which encapsidate the viral RNA.

In one embodiment, a plant viral polynucleotide is provided in which the native coat protein coding sequence has been deleted from a viral polynucleotide, a non-native plant viral coat protein coding sequence and a non-native promoter, preferably the subgenomic promoter of the non-native coat protein coding sequence, capable of expression in the plant host, packaging of the recombinant plant viral polynucleotide, and ensuring a systemic infection of the host by the recombinant plant viral polynucleotide, has been inserted. Alternatively, the coat protein gene may be inactivated by insertion of the non-native polynucleotide sequence within it, such that a protein is produced. The recombinant plant viral polynucleotide may contain one or more additional non-native subgenomic promoters. Each non-native subgenomic promoter is capable of transcribing or expressing adjacent genes or polynucleotide sequences in the plant host and incapable of recombination with each other and with native subgenomic promoters. Non-native (foreign) polynucleotide sequences may be inserted adjacent the native plant viral subgenomic promoter or the native and a non-native plant viral subgenomic promoters if more than one polynucleotide sequence is included. The non-native polynucleotide sequences are transcribed or expressed in the host plant under control of the subgenomic promoter to produce the desired products.

In a second embodiment, a recombinant plant viral polynucleotide is provided as in the first embodiment except that the native coat protein coding sequence is placed adjacent one of the non-native coat protein subgenomic promoters instead of a non-native coat protein coding sequence.

In a third embodiment, a recombinant plant viral polynucleotide is provided in which the native coat protein gene is adjacent its subgenomic promoter and one or more non-native subgenomic promoters have been inserted into the viral polynucleotide. The inserted non-native subgenomic promoters are capable of transcribing or expressing adjacent genes in a plant host and are incapable of recombination with each other and with native subgenomic promoters. Non-native polynucleotide sequences may be inserted adjacent the non-native subgenomic plant viral promoters such that the sequences are transcribed or expressed in the host plant under control of the subgenomic promoters to produce the desired product.

In a fourth embodiment, a recombinant plant viral polynucleotide is provided as in the third embodiment except that the native coat protein coding sequence is replaced by a non-native coat protein coding sequence.

The viral vectors are encapsidated by the coat proteins encoded by the recombinant plant viral polynucleotide to produce a recombinant plant virus. The recombinant plant viral polynucleotide or recombinant plant virus is used to infect appropriate host plants. The recombinant plant viral polynucleotide is capable of replication in the host, systemic spread in the host, and transcription or expression of foreign gene(s) (exogenous polynucleotide) in the host to produce the desired protein.

Techniques for inoculation of viruses to plants may be found in Foster and Taylor, eds. “Plant Virology Protocols: From Virus Isolation to Transgenic Resistance (Methods in Molecular Biology (Humana Pr), Vol 81)”, Humana Press, 1998; Maramorosh and Koprowski, eds. “Methods in Virology” 7 vols, Academic Press, New York 1967-1984; Hill, S. A. “Methods in Plant Virology”, Blackwell, Oxford, 1984; Walkey, D. G. A. “Applied Plant Virology”, Wiley, New York, 1985; and Kado and Agrawa, eds. “Principles and Techniques in Plant Virology”, Van Nostrand-Reinhold, New York.

In addition to the above, the polynucleotide of the present invention can also be introduced into a chloroplast genome thereby enabling chloroplast expression.

A technique for introducing exogenous polynucleotide sequences to the genome of the chloroplasts is known. This technique involves the following procedures. First, plant cells are chemically treated so as to reduce the number of chloroplasts per cell to about one. Then, the exogenous polynucleotide is introduced via particle bombardment into the cells with the aim of introducing at least one exogenous polynucleotide molecule into the chloroplasts. The exogenous polynucleotides selected such that it is integratable into the chloroplast's genome via homologous recombination which is readily effected by enzymes inherent to the chloroplast. To this end, the exogenous polynucleotide includes, in addition to a gene of interest, at least one polynucleotide stretch which is derived from the chloroplast's genome. In addition, the exogenous polynucleotide includes a selectable marker, which serves by sequential selection procedures to ascertain that all or substantially all of the copies of the chloroplast genomes following such selection will include the exogenous polynucleotide. Further details relating to this technique are found in U.S. Pat. Nos. 4,945,050; and 5,693,507 which are incorporated herein by reference. A polypeptide can thus be produced by the protein expression system of the chloroplast and become integrated into the chloroplast's inner membrane.

Since processes which increase nitrogen use efficiency, fertilizer use efficiency, oil content, yield, seed yield, fiber yield, fiber quality, fiber length, growth rate, photosynthetic capacity, biomass, vigor, water use efficiency, and/or abiotic stress tolerance of a plant can involve multiple genes acting additively or in synergy (see, for example, in Quesda et al., Plant Physiol. 130:951-063, 2002), the present invention also envisages expressing a plurality of exogenous polynucleotides in a single host plant to thereby achieve superior effect on nitrogen use efficiency, fertilizer use efficiency, oil content, yield, seed yield, fiber yield, fiber quality, fiber length, growth rate, photosynthetic capacity, biomass, vigor, water use efficiency, and/or abiotic stress tolerance.

Expressing a plurality of exogenous polynucleotides in a single host plant can be effected by co-introducing multiple nucleic acid constructs, each including a different exogenous polynucleotide, into a single plant cell. The transformed cell can then be regenerated into a mature plant using the methods described hereinabove.

Alternatively, expressing a plurality of exogenous polynucleotides in a single host plant can be effected by co-introducing into a single plant-cell a single nucleic-acid construct including a plurality of different exogenous polynucleotides. Such a construct can be designed with a single promoter sequence which can transcribe a polycistronic messenger RNA including all the different exogenous polynucleotide sequences. To enable co-translation of the different polypeptides encoded by the polycistronic messenger RNA, the polynucleotide sequences can be inter-linked via an internal ribosome entry site (IRES) sequence which facilitates translation of polynucleotide sequences positioned downstream of the IRES sequence. In this case, a transcribed polycistronic RNA molecule encoding the different polypeptides described above will be translated from both the capped 5′ end and the two internal IRES sequences of the polycistronic RNA molecule to thereby produce in the cell all different polypeptides. Alternatively, the construct can include several promoter sequences each linked to a different exogenous polynucleotide sequence.

The plant cell transformed with the construct including a plurality of different exogenous polynucleotides, can be regenerated into a mature plant, using the methods described hereinabove.

Alternatively, expressing a plurality of exogenous polynucleotides in a single host plant can be effected by introducing different nucleic acid constructs, including different exogenous polynucleotides, into a plurality of plants. The regenerated transformed plants can then be cross-bred and resultant progeny selected for superior abiotic stress tolerance, water use efficiency, fertilizer use efficiency, growth, biomass, yield and/or vigor traits, using conventional plant breeding techniques.

According to some embodiments of the invention, the method further comprising growing the plant expressing the exogenous polynucleotide under the abiotic stress.

Non-limiting examples of abiotic stress conditions include, salinity, osmotic stress, drought, water deprivation, excess of water (e.g., flood, waterlogging), low temperature (e.g., cold stress), high temperature, heavy metal toxicity, anaerobiosis, nutrient deficiency (e.g., nitrogen deficiency or nitrogen limitation), nutrient excess, atmospheric pollution and UV irradiation.

According to some embodiments of the invention, the method further comprising growing the plant expressing the exogenous polynucleotide under fertilizer limiting conditions (e.g., nitrogen-limiting conditions). Non-limiting examples include growing the plant on soils with low nitrogen content (40-50% Nitrogen of the content present under normal or optimal conditions), or even under sever nitrogen deficiency (0-10% Nitrogen of the content present under normal or optimal conditions).

Thus, the invention encompasses plants exogenously expressing the polynucleotide(s), the nucleic acid constructs and/or polypeptide(s) of the invention.

Once expressed within the plant cell or the entire plant, the level of the polypeptide encoded by the exogenous polynucleotide can be determined by methods well known in the art such as, activity assays, Western blots using antibodies capable of specifically binding the polypeptide, Enzyme-Linked Immuno Sorbent Assay (ELISA), radio-immuno-assays (RIA), immunohistochemistry, immunocytochemistry, immunofluorescence and the like.

Methods of determining the level in the plant of the RNA transcribed from the exogenous polynucleotide are well known in the art and include, for example, Northern blot analysis, reverse transcription polymerase chain reaction (RT-PCR) analysis (including quantitative, semi-quantitative or real-time RT-PCR) and RNA-in situ hybridization.

The sequence information and annotations uncovered by the present teachings can be harnessed in favor of classical breeding. Thus, sub-sequence data of those polynucleotides described above, can be used as markers for marker assisted selection (MAS), in which a marker is used for indirect selection of a genetic determinant or determinants of a trait of interest [e.g., abiotic stress tolerance, water use efficiency, growth rate, vigor, biomass, oil content, yield, seed yield, fiber yield, fiber quality, fiber length, photosynthetic capacity, and/or fertilizer use efficiency (e.g., nitrogen use efficiency)]. Nucleic acid data of the present teachings (DNA or RNA sequence) may contain or be linked to polymorphic sites or genetic markers on the genome such as restriction fragment length polymorphism (RFLP), microsatellites and single nucleotide polymorphism (SNP), DNA fingerprinting (DFP), amplified fragment length polymorphism (AFLP), expression level polymorphism, polymorphism of the encoded polypeptide and any other polymorphism at the DNA or RNA sequence.

Examples of marker assisted selections include, but are not limited to, selection for a morphological trait (e.g., a gene that affects form, coloration, male sterility or resistance such as the presence or absence of awn, leaf sheath coloration, height, grain color, aroma of rice); selection for a biochemical trait (e.g., a gene that encodes a protein that can be extracted and observed; for example, isozymes and storage proteins); selection for a biological trait (e.g., pathogen races or insect biotypes based on host pathogen or host parasite interaction can be used as a marker since the genetic constitution of an organism can affect its susceptibility to pathogens or parasites).

The polynucleotides and polypeptides described hereinabove can be used in a wide range of economical plants, in a safe and cost effective manner.

Plant lines exogenously expressing the polynucleotide or the polypeptide of the invention are screened to identify those that show the greatest increase of the desired plant trait.

Thus, according to an additional embodiment of the present invention, there is provided a method of evaluating a trait of a plant, the method comprising: (a) expressing in a plant or a portion thereof the nucleic acid construct of some embodiments of the invention; and (b) evaluating a trait of a plant as compared to a wild type plant of the same type (e.g., a plant not transformed with the claimed biomolecules); thereby evaluating the trait of the plant.

According to an aspect of some embodiments of the invention there is provided a method of producing a crop comprising growing a crop of a plant expressing an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or more say 100% homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs: 475-770 and 6179-10421, wherein said plant is derived from a plant selected for increased abiotic stress tolerance, increased water use efficiency, increased growth rate, increased vigor, increased biomass, increased oil content, increased yield, increased seed yield, increased fiber yield, increased fiber quality, increased fiber length, increased photosynthetic capacity, and/or increased fertilizer use efficiency (e.g., increased nitrogen use efficiency) as compared to a control plant, thereby producing the crop.

According to an aspect of some embodiments of the present invention there is provided a method of producing a crop comprising growing a crop plant transformed with an exogenous polynucleotide encoding a polypeptide at least 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or more say 100% homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs: 475-770 and 6179-10421, wherein the crop plant is derived from plants selected for increased abiotic stress tolerance, increased water use efficiency, increased growth rate, increased vigor, increased biomass, increased oil content, increased yield, increased seed yield, increased fiber yield, increased fiber quality, increased fiber length, increased photosynthetic capacity, and/or increased fertilizer use efficiency (e.g., increased nitrogen use efficiency) as compared to a wild type plant of the same species which is grown under the same growth conditions, and the crop plant having the increased abiotic stress tolerance, increased water use efficiency, increased growth rate, increased vigor, increased biomass, increased oil content, increased yield, increased seed yield, increased fiber yield, increased fiber quality, increased fiber length, increased photosynthetic capacity, and/or increased fertilizer use efficiency (e.g., increased nitrogen use efficiency), thereby producing the crop.

According to an aspect of some embodiments of the invention there is provided a method of producing a crop comprising growing a crop of a plant expressing an exogenous polynucleotide which comprises a nucleic acid sequence which is at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, e.g., 100% identical to the nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-474 and 771-6178, wherein said plant is derived from a plant selected for increased abiotic stress tolerance, increased water use efficiency, increased growth rate, increased vigor, increased biomass, increased oil content, increased yield, increased seed yield, increased fiber yield, increased fiber quality, increased fiber length, increased photosynthetic capacity, and/or increased fertilizer use efficiency (e.g., increased nitrogen use efficiency) as compared to a control plant, thereby producing the crop.

According to an aspect of some embodiments of the present invention there is provided a method of producing a crop comprising growing a crop plant transformed with an exogenous polynucleotide at least 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or more say 100% identical to the nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-474 and 771-6178, wherein the crop plant is derived from plants selected for increased abiotic stress tolerance, increased water use efficiency, increased growth rate, increased vigor, increased biomass, increased oil content, increased yield, increased seed yield, increased fiber yield, increased fiber quality, increased fiber length, increased photosynthetic capacity, and/or increased fertilizer use efficiency (e.g., increased nitrogen use efficiency) as compared to a wild type plant of the same species which is grown under the same growth conditions, and the crop plant having the increased abiotic stress tolerance, increased water use efficiency, increased growth rate, increased vigor, increased biomass, increased oil content, increased yield, increased seed yield, increased fiber yield, increased fiber quality, increased fiber length, increased photosynthetic capacity, and/or increased fertilizer use efficiency (e.g., increased nitrogen use efficiency), thereby producing the crop.

According to an aspect of some embodiments of the invention there is provided a method of growing a crop comprising seeding seeds and/or planting plantlets of a plant transformed with the exogenous polynucleotide of the invention, e.g., the polynucleotide which encodes the polypeptide of some embodiments of the invention, wherein the plant is derived from plants selected for at least one trait selected from the group consisting of increased abiotic stress tolerance, increased water use efficiency, increased growth rate, increased vigor, increased biomass, increased oil content, increased yield, increased seed yield, increased fiber yield, increased fiber quality, increased fiber length, increased photosynthetic capacity, and/or increased fertilizer use efficiency (e.g., increased nitrogen use efficiency) as compared to a non-transformed plant.

According to some embodiments of the invention the method of growing a crop comprising seeding seeds and/or planting plantlets of a plant transformed with an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, e.g., 100% identical to the nucleic acid sequence set forth in SEQ ID NO: 475-770 and 6179-10421, wherein the plant is derived from plants selected for at least one trait selected from the group consisting of increased abiotic stress tolerance, increased water use efficiency, increased growth rate, increased vigor, increased biomass, increased oil content, increased yield, increased seed yield, increased fiber yield, increased fiber quality, increased fiber length, increased photosynthetic capacity, and/or increased fertilizer use efficiency (e.g., increased nitrogen use efficiency) as compared to a non-transformed plant, thereby growing the crop.

According to some embodiments of the invention the method of growing a crop comprising seeding seeds and/or planting plantlets of a plant transformed with an exogenous polynucleotide comprising the nucleic acid sequence at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, e.g., 100% identical to the nucleic acid sequence set forth in SEQ ID NO: 1-474 and 771-6178, wherein the plant is derived from plants selected for at least one trait selected from the group consisting of increased abiotic stress tolerance, increased water use efficiency, increased growth rate, increased vigor, increased biomass, increased oil content, increased yield, increased seed yield, increased fiber yield, increased fiber quality, increased fiber length, increased photosynthetic capacity, and/or increased fertilizer use efficiency (e.g., increased nitrogen use efficiency) as compared to a non-transformed plant, thereby growing the crop.

The effect of the transgene (the exogenous polynucleotide encoding the polypeptide) on abiotic stress tolerance can be determined using known methods such as detailed below and in the Examples section which follows.

Abiotic stress tolerance—Transformed (i.e., expressing the transgene) and non-transformed (wild type) plants are exposed to an abiotic stress condition, such as water deprivation, suboptimal temperature (low temperature, high temperature), nutrient deficiency, nutrient excess, a salt stress condition, osmotic stress, heavy metal toxicity, anaerobiosis, atmospheric pollution and UV irradiation.

Salinity tolerance assay—Transgenic plants with tolerance to high salt concentrations are expected to exhibit better germination, seedling vigor or growth in high salt. Salt stress can be effected in many ways such as, for example, by irrigating the plants with a hyperosmotic solution, by cultivating the plants hydroponically in a hyperosmotic growth solution (e.g., Hoagland solution), or by culturing the plants in a hyperosmotic growth medium [e.g., 50% Murashige-Skoog medium (MS medium)]. Since different plants vary considerably in their tolerance to salinity, the salt concentration in the irrigation water, growth solution, or growth medium can be adjusted according to the specific characteristics of the specific plant cultivar or variety, so as to inflict a mild or moderate effect on the physiology and/or morphology of the plants (for guidelines as to appropriate concentration see, Bernstein and Kafkafi, Root Growth Under Salinity Stress In: Plant Roots, The Hidden Half 3rd ed. Waisel Y, Eshel A and Kafkafi U. (editors) Marcel Dekker Inc., New York, 2002, and reference therein).

For example, a salinity tolerance test can be performed by irrigating plants at different developmental stages with increasing concentrations of sodium chloride (for example 50 mM, 100 mM, 200 mM, 400 mM NaCl) applied from the bottom and from above to ensure even dispersal of salt. Following exposure to the stress condition the plants are frequently monitored until substantial physiological and/or morphological effects appear in wild type plants. Thus, the external phenotypic appearance, degree of wilting and overall success to reach maturity and yield progeny are compared between control and transgenic plants.

Quantitative parameters of tolerance measured include, but are not limited to, the average wet and dry weight, growth rate, leaf size, leaf coverage (overall leaf area), the weight of the seeds yielded, the average seed size and the number of seeds produced per plant. Transformed plants not exhibiting substantial physiological and/or morphological effects, or exhibiting higher biomass than wild-type plants, are identified as abiotic stress tolerant plants.

Osmotic tolerance test—Osmotic stress assays (including sodium chloride and mannitol assays) are conducted to determine if an osmotic stress phenotype was sodium chloride-specific or if it was a general osmotic stress related phenotype. Plants which are tolerant to osmotic stress may have more tolerance to drought and/or freezing. For salt and osmotic stress germination experiments, the medium is supplemented for example with 50 mM, 100 mM, 200 mM NaCl or 100 mM, 200 mM NaCl, 400 mM mannitol.

Drought tolerance assay/Osmoticum assay—Tolerance to drought is performed to identify the genes conferring better plant survival after acute water deprivation. To analyze whether the transgenic plants are more tolerant to drought, an osmotic stress produced by the non-ionic osmolyte sorbitol in the medium can be performed. Control and transgenic plants are germinated and grown in plant-agar plates for 4 days, after which they are transferred to plates containing 500 mM sorbitol. The treatment causes growth retardation, then both control and transgenic plants are compared, by measuring plant weight (wet and dry), yield, and by growth rates measured as time to flowering.

Conversely, soil-based drought screens are performed with plants overexpressing the polynucleotides detailed above. Seeds from control Arabidopsis plants, or other transgenic plants overexpressing the polypeptide of the invention are germinated and transferred to pots. Drought stress is obtained after irrigation is ceased accompanied by placing the pots on absorbent paper to enhance the soil-drying rate. Transgenic and control plants are compared to each other when the majority of the control plants develop severe wilting. Plants are re-watered after obtaining a significant fraction of the control plants displaying a severe wilting. Plants are ranked comparing to controls for each of two criteria: tolerance to the drought conditions and recovery (survival) following re-watering.

The maintenance of a phenotype under drought condition as compared to normal conditions can be determined as the “RATIO Drought/Normal”, which represents ratio for the specified parameter of Drought condition results (measured parameter under Drought) divided by measured parameter under Normal conditions.

Cold stress tolerance—To analyze cold stress, mature (25 day old) plants are transferred to 4° C. chambers for 1 or 2 weeks, with constitutive light. Later on plants are moved back to greenhouse. Two weeks later damages from chilling period, resulting in growth retardation and other phenotypes, are compared between both control and transgenic plants, by measuring plant weight (wet and dry), and by comparing growth rates measured as time to flowering, plant size, yield, and the like.

Heat stress tolerance—Heat stress tolerance is achieved by exposing the plants to temperatures above 34° C. for a certain period. Plant tolerance is examined after transferring the plants back to 22° C. for recovery and evaluation after 5 days relative to internal controls (non-transgenic plants) or plants not exposed to neither cold or heat stress.

Water use efficiency—can be determined as the biomass produced per unit transpiration. To analyze WUE, leaf relative water content can be measured in control and transgenic plants. Fresh weight (FW) is immediately recorded; then leaves are soaked for 8 hours in distilled water at room temperature in the dark, and the turgid weight (TW) is recorded. Total dry weight (DW) is recorded after drying the leaves at 60° C. to a constant weight. Relative water content (RWC) is calculated according to the following Formula I:

RWC=[(FW−DW)/(TW−DW)]×100 Formula I

Fertilizer use efficiency—To analyze whether the transgenic plants are more responsive to fertilizers, plants are grown in agar plates or pots with a limited amount of fertilizer, as described, for example, in Yanagisawa et al (Proc Natl Acad Sci USA. 2004; 101:7833-8). The plants are analyzed for their overall size, time to flowering, yield, protein content of shoot and/or grain. The parameters checked are the overall size of the mature plant, its wet and dry weight, the weight of the seeds yielded, the average seed size and the number of seeds produced per plant. Other parameters that may be tested are: the chlorophyll content of leaves (as nitrogen plant status and the degree of leaf verdure is highly correlated), amino acid and the total protein content of the seeds or other plant parts such as leaves or shoots, oil content, etc. Similarly, instead of providing nitrogen at limiting amounts, phosphate or potassium can be added at increasing concentrations. Again, the same parameters measured are the same as listed above. In this way, nitrogen use efficiency (NUE), phosphate use efficiency (PUE) and potassium use efficiency (KUE) are assessed, checking the ability of the transgenic plants to thrive under nutrient restraining conditions.

Nitrogen use efficiency—To analyze whether the transgenic plants (e.g., Arabidopsis plants) are more responsive to nitrogen, plant are grown in 0.75-3 mM (nitrogen deficient conditions) or 6-10 mM (optimal nitrogen concentration). Plants are allowed to grow for additional 25 days or until seed production. The plants are then analyzed for their overall size, time to flowering, yield, protein content of shoot and/or grain/seed production. The parameters checked can be the overall size of the plant, wet and dry weight, the weight of the seeds yielded, the average seed size and the number of seeds produced per plant. Other parameters that may be tested are: the chlorophyll content of leaves (as nitrogen plant status and the degree of leaf greenness is highly correlated), amino acid and the total protein content of the seeds or other plant parts such as leaves or shoots and oil content. Transformed plants not exhibiting substantial physiological and/or morphological effects, or exhibiting higher measured parameters levels than wild-type plants, are identified as nitrogen use efficient plants.

Nitrogen Use efficiency assay using plantlets—The assay is done according to Yanagisawa-S. et al. with minor modifications (“Metabolic engineering with Dof1 transcription factor in plants: Improved nitrogen assimilation and growth under low-nitrogen conditions” Proc. Natl. Acad. Sci. USA 101, 7833-7838). Briefly, transgenic plants which are grown for 7-10 days in 0.5×MS [Murashige-Skoog] supplemented with a selection agent are transferred to two nitrogen-limiting conditions: MS media in which the combined nitrogen concentration (NH₄NO₃and KNO₃) was 0.75 mM (nitrogen deficient conditions) or 6-15 mM (optimal nitrogen concentration). Plants are allowed to grow for additional 30-40 days and then photographed, individually removed from the Agar (the shoot without the roots) and immediately weighed (fresh weight) for later statistical analysis. Constructs for which only T1 seeds are available are sown on selective media and at least 20 seedlings (each one representing an independent transformation event) are carefully transferred to the nitrogen-limiting media. For constructs for which T2 seeds are available, different transformation events are analyzed. Usually, 20 randomly selected plants from each event are transferred to the nitrogen-limiting media allowed to grow for 3-4 additional weeks and individually weighed at the end of that period. Transgenic plants are compared to control plants grown in parallel under the same conditions. Mock-transgenic plants expressing the uidA reporter gene (GUS) under the same promoter or transgenic plants carrying the same promoter but lacking a reporter gene are used as control.

Nitrogen determination—The procedure for N (nitrogen) concentration determination in the structural parts of the plants involves the potassium persulfate digestion method to convert organic N to NO₃⁻(Purcell and King 1996 Argon. J. 88:111-113, the modified Cd⁻ mediated reduction of NO₃⁻to NO₂⁻ (Vodovotz 1996 Biotechniques 20:390-394) and the measurement of nitrite by the Griess assay (Vodovotz 1996, supra). The absorbance values are measured at 550 nm against a standard curve of NaNO₂. The procedure is described in details in Samonte et al. 2006 Agron. J. 98:168-176.

Germination tests—Germination tests compare the percentage of seeds from transgenic plants that could complete the germination process to the percentage of seeds from control plants that are treated in the same manner Normal conditions are considered for example, incubations at 22° C. under 22-hour light 2-hour dark daily cycles. Evaluation of germination and seedling vigor is conducted between 4 and 14 days after planting. The basal media is 50% MS medium (Murashige and Skoog, 1962 Plant Physiology 15, 473-497).

Germination is checked also at unfavorable conditions such as cold (incubating at temperatures lower than 10° C. instead of 22° C.) or using seed inhibition solutions that contain high concentrations of an osmolyte such as sorbitol (at concentrations of 50 mM, 100 mM, 200 mM, 300 mM, 500 mM, and up to 1000 mM) or applying increasing concentrations of salt (of 50 mM, 100 mM, 200 mM, 300 mM, 500 mM NaCl).

The effect of the transgene on plant's vigor, growth rate, biomass, yield and/or oil content can be determined using known methods.

Plant vigor—The plant vigor can be calculated by the increase in growth parameters such as leaf area, fiber length, rosette diameter, plant fresh weight and the like per time.

Growth rate—The growth rate can be measured using digital analysis of growing plants. For example, images of plants growing in greenhouse on plot basis can be captured every 3 days and the rosette area can be calculated by digital analysis. Rosette area growth is calculated using the difference of rosette area between days of sampling divided by the difference in days between samples.

Evaluation of growth rate can be done by measuring plant biomass produced, rosette area, leaf size or root length per time (can be measured in cm²per day of leaf area).

Relative growth area (RGR) can be calculated using Formula II.

Relative growth rate area=Regression coefficient of area along time course Formula II:

Thus, the relative growth area rate is in units of area units (e g, mm²/day or cm²/day) and the length growth rate is in units of length units (e.g., cm/day or mm/day).

For example, RGR can be determined for plant height (Formula IX), SPAD (Formula XIII), Number of tillers (Formula XIV), root length (Formula XVII), vegetative growth (Formula XVII), leaf number (Formula XXI), rosette area (Formula XXII), rosette diameter (Formula XXIII), plot coverage (Formula XXIV), leaf blade area (Formula XX), and leaf area (Formula XXVI).

Relative growth rate of Plant height=Regression coefficient of Plant height along time course (measured in cm/day). Formula IX:

Relative growth rate of SPAD=Regression coefficient of SPAD Formula XIII: measurements along time course.

Relative growth rate of Number of tillers=Regression coefficient of Number of tillers along time course (measured in units of “number of tillers/day”). Formula XIV:

Relative growth rate of root length=Regression coefficient of root length along time course (measured in cm per day). Formula XVII:

Vegetative growth rate analysis—was calculated according to Formula XVII below.

Relative growth rate of vegetative growth=Regression coefficient of leaf area along time course (measured in cm²per day). Formula XVII:

Relative growth rate of leaf number=Regression coefficient of leaf number along time course (measured in number per day). Formula XXI:

Relative growth rate of rosette area=Regression coefficient of rosette area along time course (measured in cm²per day). Formula XXII:

Relative growth rate of rosette diameter=Regression coefficient of rosette diameter along time course (measured in cm per day). Formula XXIII:

Relative growth rate of plot coverage=Regression coefficient of plot (measured in cm²per day). Formula XXIV:

Relative growth rate of leaf blade area=Regression coefficient of leaf area along time course (measured in cm²per day). Formula XX:

Relative growth rate of leaf area=Regression coefficient of leaf area along time course (measured in cm²per day). Formula XXVI:

Seed yield—Evaluation of the seed yield per plant can be done by measuring the amount (weight or size) or quantity (i.e., number) of dry seeds produced and harvested from 8-16 plants and divided by the number of plants.

For example, the total seeds from 8-16 plants can be collected, weighted using e.g., an analytical balance and the total weight can be divided by the number of plants. Seed yield per growing area can be calculated in the same manner while taking into account the growing area given to a single plant. Increase seed yield per growing area could be achieved by increasing seed yield per plant, and/or by increasing number of plants capable of growing in a given area.

In addition, seed yield can be determined via the weight of 1000 seeds. The weight of 1000 seeds can be determined as follows: seeds are scattered on a glass tray and a picture is taken. Each sample is weighted and then using the digital analysis, the number of seeds in each sample is calculated.

The 1000 seeds weight can be calculated using formula III:

1000 Seed Weight=number of seed in sample/sample weight×1000 Formula III:

The Harvest Index can be calculated using Formulas IV, V, VIII and XI below.

Harvest Index (seed)=Average seed yield per plant/Average dry weight Formula IV:

Harvest Index (Sorghum)=Average grain dry weight per Head/(Average vegetative dry weight per Head+Average Head dry weight) Formula V:

Harvest Index (Maize)=Average grain weight per plant/(Average vegetative dry weight per plant plus Average grain weight per plant) Formula VIII:

Harvest Index (for barley)—The harvest index is calculated using Formula XI.

Harvest Index (barley)=Average spike dry weight per plant/(Average vegetative dry weight per plant+Average spike dry weight per plant) Formula XI:

Following is a non-limited list of additional parameters which can be detected in order to show the effect of the transgene on the desired plant's traits.

Grain circularity=4×3.14(grain area/perimeter²) Formula VI:

internode volume=3.14×(d/2)²×1 Formula VII:

Normalized ear weight per plant+vegetative dry weight. Formula X:

Root/Shoot Ratio=total weight of the root at harvest/total weight of the vegetative portion above ground at harvest. Formula XII:

Ratio of the number of pods per node on main stem at pod set=Total number of pods on main stem/Total number of nodes on main stem, average of three plants per plot. Formula XV:

Ratio of total number of seeds in main stem to number of seeds on lateral branches=Total number of seeds on main stem at pod set/Total number of seeds on lateral branches at pod set. Formula XVI:

Petiole Relative Area=(Petiol area)/Rosette area (measured in %). Formula XXV:

Grain protein concentration—Grain protein content (g grain protein m⁻²) is estimated as the product of the mass of grain N (g grain N m⁻²) multiplied by the N/protein conversion ratio of k-5.13 (Mosse 1990, supra). The grain protein concentration is estimated as the ratio of grain protein content per unit mass of the grain (g grain protein kg⁻¹grain).

Fiber length—Fiber length can be measured using fibrograph. The fibrograph system was used to compute length in terms of “Upper Half Mean” length. The upper half mean (UHM) is the average length of longer half of the fiber distribution. The fibrograph measures length in span lengths at a given percentage point (Hypertext Transfer Protocol://World Wide Web (dot) cottoninc (dot) com/ClassificationofCotton/?Pg=4#Length).

According to some embodiments of the invention, increased yield of corn may be manifested as one or more of the following: increase in the number of plants per growing area, increase in the number of ears per plant, increase in the number of rows per ear, number of kernels per ear row, kernel weight, thousand kernel weight (1000-weight), ear length/diameter, increase oil content per kernel and increase starch content per kernel.

As mentioned, the increase of plant yield can be determined by various parameters. For example, increased yield of rice may be manifested by an increase in one or more of the following: number of plants per growing area, number of panicles per plant, number of spikelets per panicle, number of flowers per panicle, increase in the seed filling rate, increase in thousand kernel weight (1000-weight), increase oil content per seed, increase starch content per seed, among others. An increase in yield may also result in modified architecture, or may occur because of modified architecture.

Similarly, increased yield of soybean may be manifested by an increase in one or more of the following: number of plants per growing area, number of pods per plant, number of seeds per pod, increase in the seed filling rate, increase in thousand seed weight (1000-weight), reduce pod shattering, increase oil content per seed, increase protein content per seed, among others. An increase in yield may also result in modified architecture, or may occur because of modified architecture.

Increased yield of canola may be manifested by an increase in one or more of the following: number of plants per growing area, number of pods per plant, number of seeds per pod, increase in the seed filling rate, increase in thousand seed weight (1000-weight), reduce pod shattering, increase oil content per seed, among others. An increase in yield may also result in modified architecture, or may occur because of modified architecture.

Increased yield of cotton may be manifested by an increase in one or more of the following: number of plants per growing area, number of bolls per plant, number of seeds per boll, increase in the seed filling rate, increase in thousand seed weight (1000-weight), increase oil content per seed, improve fiber length, fiber strength, among others. An increase in yield may also result in modified architecture, or may occur because of modified architecture.

Oil content—The oil content of a plant can be determined by extraction of the oil from the seed or the vegetative portion of the plant. Briefly, lipids (oil) can be removed from the plant (e.g., seed) by grinding the plant tissue in the presence of specific solvents (e.g., hexane or petroleum ether) and extracting the oil in a continuous extractor. Indirect oil content analysis can be carried out using various known methods such as Nuclear Magnetic Resonance (NMR) Spectroscopy, which measures the resonance energy absorbed by hydrogen atoms in the liquid state of the sample [See for example, Conway T F. and Earle F R., 1963, Journal of the American Oil Chemists' Society; Springer Berlin/Heidelberg, ISSN: 0003-021X (Print) 1558-9331 (Online)]; the Near Infrared (NI) Spectroscopy, which utilizes the absorption of near infrared energy (1100-2500 nm) by the sample; and a method described in WO/2001/023884, which is based on extracting oil a solvent, evaporating the solvent in a gas stream which forms oil particles, and directing a light into the gas stream and oil particles which forms a detectable reflected light.

Seed Oil yield can be calculated as follows: Seed yield per plant (gr.)*Oil % in seed.

Thus, the present invention is of high agricultural value for promoting the yield of commercially desired crops (e.g., biomass of vegetative organ such as poplar wood, or reproductive organ such as number of seeds or seed biomass).

Any of the transgenic plants described hereinabove or parts thereof may be processed to produce a feed, meal, protein or oil preparation, such as for ruminant animals.

The transgenic plants described hereinabove, which exhibit an increased oil content can be used to produce plant oil (by extracting the oil from the plant).

The plant oil (including the seed oil and/or the vegetative portion oil) produced according to the method of the invention may be combined with a variety of other ingredients. The specific ingredients included in a product are determined according to the intended use. Exemplary products include animal feed, raw material for chemical modification, biodegradable plastic, blended food product, edible oil, biofuel, cooking oil, lubricant, biodiesel, snack food, cosmetics, and fermentation process raw material. Exemplary products to be incorporated to the plant oil include animal feeds, human food products such as extruded snack foods, breads, as a food binding agent, aquaculture feeds, fermentable mixtures, food supplements, sport drinks, nutritional food bars, multi-vitamin supplements, diet drinks, and cereal foods.

According to some embodiments of the invention, the oil comprises a seed oil.

According to some embodiments of the invention, the oil comprises a vegetative portion oil (oil of the vegetative portion of the plant).

According to some embodiments of the invention, the plant cell forms a part of a plant.

According to another embodiment of the present invention, there is provided a food or feed comprising the plants or a portion thereof of the present invention.

As used herein the term “about” refers to ±10%.

The terms “comprises”, “comprising”, “includes”, “including”, “having” and their conjugates mean “including but not limited to”.

The term “consisting of” means “including and limited to”.

The term “consisting essentially of” means that the composition, method or structure may include additional ingredients, steps and/or parts, but only if the additional ingredients, steps and/or parts do not materially alter the basic and novel characteristics of the claimed composition, method or structure.

As used herein, the singular form “a”, “an” and “the” include plural references unless the context clearly dictates otherwise. For example, the term “a compound” or “at least one compound” may include a plurality of compounds, including mixtures thereof.

Throughout this application, various embodiments of this invention may be presented in a range format. It should be understood that the description in range format is merely for convenience and brevity and should not be construed as an inflexible limitation on the scope of the invention. Accordingly, the description of a range should be considered to have specifically disclosed all the possible subranges as well as individual numerical values within that range. For example, description of a range such as from 1 to 6 should be considered to have specifically disclosed subranges such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6 etc., as well as individual numbers within that range, for example, 1, 2, 3, 4, 5, and 6. This applies regardless of the breadth of the range.

Whenever a numerical range is indicated herein, it is meant to include any cited numeral (fractional or integral) within the indicated range. The phrases “ranging/ranges between” a first indicate number and a second indicate number and “ranging/ranges from” a first indicate number “to” a second indicate number are used herein interchangeably and are meant to include the first and second indicated numbers and all the fractional and integral numerals therebetween.

As used herein the term “method” refers to manners, means, techniques and procedures for accomplishing a given task including, but not limited to, those manners, means, techniques and procedures either known to, or readily developed from known manners, means, techniques and procedures by practitioners of the chemical, pharmacological, biological, biochemical and medical arts.

When reference is made to particular sequence listings, such reference is to be understood to also encompass sequences that substantially correspond to its complementary sequence as including minor sequence variations, resulting from, e.g., sequencing errors, cloning errors, or other alterations resulting in base substitution, base deletion or base addition, provided that the frequency of such variations is less than 1 in 50 nucleotides, alternatively, less than 1 in 100 nucleotides, alternatively, less than 1 in 200 nucleotides, alternatively, less than 1 in 500 nucleotides, alternatively, less than 1 in 1000 nucleotides, alternatively, less than 1 in 5,000 nucleotides, alternatively, less than 1 in 10,000 nucleotides.

It is appreciated that certain features of the invention, which are, for clarity, described in the context of separate embodiments, may also be provided in combination in a single embodiment. Conversely, various features of the invention, which are, for brevity, described in the context of a single embodiment, may also be provided separately or in any suitable subcombination or as suitable in any other described embodiment of the invention. Certain features described in the context of various embodiments are not to be considered essential features of those embodiments, unless the embodiment is inoperative without those elements.

Various embodiments and aspects of the present invention as delineated hereinabove and as claimed in the claims section below find experimental support in the following examples.

EXAMPLES

Reference is now made to the following examples, which together with the above descriptions illustrate some embodiments of the invention in a non limiting fashion.

Generally, the nomenclature used herein and the laboratory procedures utilized in the present invention include molecular, biochemical, microbiological and recombinant DNA techniques. Such techniques are thoroughly explained in the literature. See, for example, “Molecular Cloning: A laboratory Manual” Sambrook et al., (1989); “Current Protocols in Molecular Biology” Volumes I-III Ausubel, R. M., ed. (1994); Ausubel et al., “Current Protocols in Molecular Biology”, John Wiley and Sons, Baltimore, Md. (1989); Perbal, “A Practical Guide to Molecular Cloning”, John Wiley & Sons, New York (1988); Watson et al., “Recombinant DNA”, Scientific American Books, New York; Birren et al. (eds) “Genome Analysis: A Laboratory Manual Series”, Vols. 1-4, Cold Spring Harbor Laboratory Press, New York (1998); methodologies as set forth in U.S. Pat. Nos. 4,666,828; 4,683,202; 4,801,531; 5,192,659 and 5,272,057; “Cell Biology: A Laboratory Handbook”, Volumes I-III Cellis, J. E., ed. (1994); “Current Protocols in Immunology” Volumes I-III Coligan J. E., ed. (1994); Stites et al. (eds), “Basic and Clinical Immunology” (8th Edition), Appleton & Lange, Norwalk, Conn. (1994); Mishell and Shiigi (eds), “Selected Methods in Cellular Immunology”, W. H. Freeman and Co., New York (1980); available immunoassays are extensively described in the patent and scientific literature, see, for example, U.S. Pat. Nos. 3,791,932; 3,839,153; 3,850,752; 3,850,578; 3,853,987; 3,867,517; 3,879,262; 3,901,654; 3,935,074; 3,984,533; 3,996,345; 4,034,074; 4,098,876; 4,879,219; 5,011,771 and 5,281,521; “Oligonucleotide Synthesis” Gait, M. J., ed. (1984); “Nucleic Acid Hybridization” Hames, B. D., and Higgins S. J., eds. (1985); “Transcription and Translation” Hames, B. D., and Higgins S. J., Eds. (1984); “Animal Cell Culture” Freshney, R. I., ed. (1986); “Immobilized Cells and Enzymes” IRL Press, (1986); “A Practical Guide to Molecular Cloning” Perbal, B., (1984) and “Methods in Enzymology” Vol. 1-317, Academic Press; “PCR Protocols: A Guide To Methods And Applications”, Academic Press, San Diego, Calif. (1990); Marshak et al., “Strategies for Protein Purification and Characterization—A Laboratory Course Manual” CSHL Press (1996); all of which are incorporated by reference as if fully set forth herein. Other general references are provided throughout this document. The procedures therein are believed to be well known in the art and are provided for the convenience of the reader. All the information contained therein is incorporated herein by reference.

General Experimental and Bioinformatics Methods

RNA extraction—Tissues growing at various growth conditions (as described below) were sampled and RNA was extracted using TRIzol Reagent from Invitrogen [Hypertext Transfer Protocol://World Wide Web (dot) invitrogen (dot) com/content (dot)cfm?pageid=469]. Approximately 30-50 mg of tissue was taken from samples. The weighed tissues were ground using pestle and mortar in liquid nitrogen and resuspended in 500 μl of TRIzol Reagent. To the homogenized lysate, 100 μl of chloroform was added followed by precipitation using isopropanol and two washes with 75% ethanol. The RNA was eluted in 30 μl of RNase-free water. RNA samples were cleaned up using Qiagen's RNeasy minikit clean-up protocol as per the manufacturer's protocol (QIAGEN Inc, CA USA). For convenience, each micro-array expression information tissue type has received an expression Set ID.

Correlation analysis—was performed for selected genes according to some embodiments of the invention, in which the characterized parameters (measured parameters according to the correlation IDs) were used as “x axis” for correlation with the tissue transcriptom which was used as the “Y axis”. For each gene and measured parameter a correlation coefficient “R” was calculated (using Pearson correlation) along with a p-value for the significance of the correlation. When the correlation coefficient (R) between the levels of a gene's expression in a certain tissue and a phenotypic performance across ecotypes/variety/hybrid is high in absolute value (between 0.5-1), there is an association between the gene (specifically the expression level of this gene) the phenotypic characteristic (e.g., improved nitrogen use efficiency, abiotic stress tolerance, yield, growth rate and the like).

Example 1

Bio-Informatics Tools for Identification of Genes which Increase Abiotic Stress Tolerance, Yield and Agronomical Important Traits in Plants

The present inventors have identified polynucleotides which upregulation of expression thereof can increase abiotic stress tolerance (AB ST), water use efficiency (WUE), yield, oil content, growth rate, vigor, biomass, fiber yield and quality, nitrogen use efficiency (NUE), and fertilizer use efficiency (FUE) of a plant.

All nucleotide sequence datasets used here were originated from publicly available databases or from performing sequencing using the Solexa technology (e.g. Barley and Sorghum). Sequence data from 100 different plant species was introduced into a single, comprehensive database. Other information on gene expression, protein annotation, enzymes and pathways were also incorporated. Major databases used include:

Genomes

- Arabidopsis genome [TAIR genome version 6 (Hypertext Transfer Protocol://World Wide Web (dot) arabidopsis (dot) org/)]
- Rice genome [IRGSP build 4.0 (Hypertext Transfer Protocol://rgp (dot) dna (dot) affrc (dot) go (dot) jp/IRGSP/)].
- Poplar [Populus trichocarpa release 1.1 from JGI (assembly release v1.0) (Hypertext Transfer Protocol://World Wide Web (dot) genome (dot) jgi-psf (dot) org/)]
- Brachypodium [JGI 4x assembly, Hypertext Transfer Protocol://World Wide Web (dot) brachpodium (dot) org)]
- Soybean [DOE-JGI SCP, version GlymaO (Hypertext Transfer Protocol://World Wide Web (dot) phytozome (dot) net/)]
- Grape [French-Italian Public Consortium for Grapevine Genome Characterization grapevine genome (Hypertext Transfer Protocol://World Wide Web (dot) genoscope (dot) cns (dot) fr/)]
- Castobean [TIGR/J Craig Venter Institute 4x assembly [(Hypertext Transfer Protocol://msc (dot) jcvi (dot) org/r_communis]
- Sorghum [DOE-JGI SCP, version Sbi 1 [Hypertext Transfer Protocol://World Wide Web (dot) phytozome (dot) net/)].
- Partially assembled genome of Maize [Hypertext Transfer Protocol://maizesequence (dot) org/]

Expressed EST and mRNA sequences were extracted from the following databases:

- GenBank versions 154, 157, 160, 161, 164, 165, 166 and 168 (Hypertext Transfer Protocol://World Wide Web (dot) ncbi (dot) nlm (dot) nih (dot) gov/dbEST/)
- RefSeq (Hypertext Transfer Protocol://World Wide Web (dot) ncbi (dot) nlm (dot) nih (dot) gov/RefSeq/).
- TAIR (Hypertext Transfer Protocol://World Wide Web (dot) arabidopsis (dot) org/).

Protein and pathway databases

- Uniprot [Hypertext Transfer Protocol://World Wide Web (dot) uniprot (dot) org/].
- AraCyc [Hypertext Transfer Protocol://World Wide Web (dot) arabidopsis (dot) org/biocyc/index (dot) jsp].
- ENZYME [Hypertext Transfer Protocol://expasy (dot) org/enzyme/].

Microarray datasets were downloaded from:

- GEO (Hypertext Transfer Protocol://World Wide Web.ncbi.nlm.nih.gov/geo/)
- TAIR (Hypertext Transfer Protocol://World Wide Web.arabidopsis.org/).
- Proprietary microarray data (WO2008/122980 and Example 2 below).

QTL and SNPs information

- Gramene [Hypertext Transfer Protocol://World Wide Web (dot) gramene (dot) org/qtl/].
- Panzea [Hypertext Transfer Protocol://World Wide Web (dot) panzea (dot) org/index (dot) html].

Database assembly—was performed to build a wide, rich, reliable annotated and easy to analyze database comprised of publicly available genomic mRNA, ESTs DNA sequences, data from various crops as well as gene expression, protein annotation and pathway data QTLs, and other relevant information.

Database assembly is comprised of a toolbox of gene refining, structuring, annotation and analysis tools enabling to construct a tailored database for each gene discovery project. Gene refining and structuring tools enable to reliably detect splice variants and antisense transcripts, generating understanding of various potential phenotypic outcomes of a single gene. The capabilities of the “LEADS” platform of Compugen LTD for analyzing human genome have been confirmed and accepted by the scientific community [see e.g., “Widespread Antisense Transcription”, Yelin, et al. (2003) Nature Biotechnology 21, 379-85; “Splicing of Alu Sequences”, Lev-Maor, et al. (2003) Science 300 (5623), 1288-91; “Computational analysis of alternative splicing using EST tissue information”, Xie H et al. Genomics 2002], and have been proven most efficient in plant genomics as well.

EST clustering and gene assembly—For gene clustering and assembly of organisms with available genome sequence data (arabidopsis, rice, castorbean, grape, brachypodium, poplar, soybean, sorghum) the genomic LEADS version (GANG) was employed. This tool allows most accurate clustering of ESTs and mRNA sequences on genome, and predicts gene structure as well as alternative splicing events and anti-sense transcription.

For organisms with no available full genome sequence data, “expressed LEADS” clustering software was applied.

Gene annotation—Predicted genes and proteins were annotated as follows:

Blast search [Hypertext Transfer Protocol://blast (dot) ncbi (dot) nlm (dot) nih (dot) gov/Blast (dot) cgi] against all plant UniProt [Hypertext Transfer Protocol://World Wide Web (dot) uniprot (dot) org/] sequences was performed. Open reading frames of each putative transcript were analyzed and longest ORF with higher number of homologues was selected as predicted protein of the transcript. The predicted proteins were analyzed by InterPro [Hypertext Transfer Protocol://World Wide Web (dot) ebi (dot) ac (dot) uk/interpro/].

Blast against proteins from AraCyc and ENZYME databases was used to map the predicted transcripts to AraCyc pathways.

Predicted proteins from different species were compared using blast algorithm [Hypertext Transfer Protocol://World Wide Web (dot) ncbi (dot) nlm (dot) nih (dot) gov/Blast (dot) cgi] to validate the accuracy of the predicted protein sequence, and for efficient detection of orthologs.

Gene expression profiling—Several data sources were exploited for gene expression profiling, namely microarray data and digital expression profile (see below). According to gene expression profile, a correlation analysis was performed to identify genes which are co-regulated under different development stages and environmental conditions and associated with different phenotypes.

Publicly available microarray datasets were downloaded from TAIR and NCBI GEO sites, renormalized, and integrated into the database. Expression profiling is one of the most important resource data for identifying genes important for ABST, increased yield, growth rate, vigor, biomass, oil content, WUE, NUE and FUE of a plant.

A digital expression profile summary was compiled for each cluster according to all keywords included in the sequence records comprising the cluster. Digital expression, also known as electronic Northern Blot, is a tool that displays virtual expression profile based on the EST sequences forming the gene cluster. The tool provides the expression profile of a cluster in terms of plant anatomy (e.g., the tissue/organ in which the gene is expressed), developmental stage (the developmental stages at which a gene can be found) and profile of treatment (provides the physiological conditions under which a gene is expressed such as drought, cold, pathogen infection, etc). Given a random distribution of ESTs in the different clusters, the digital expression provides a probability value that describes the probability of a cluster having a total of N ESTs to contain X ESTs from a certain collection of libraries. For the probability calculations, the following is taken into consideration: a) the number of ESTs in the cluster, b) the number of ESTs of the implicated and related libraries, c) the overall number of ESTs available representing the species. Thereby clusters with low probability values are highly enriched with ESTs from the group of libraries of interest indicating a specialized expression.

Recently, the accuracy of this system was demonstrated by Portnoy et al., 2009 (Analysis Of The Melon Fruit Transcriptome Based On 454 Pyrosequencing) in: Plant & Animal Genomes XVII Conference, San Diego, Calif. Transcriptomic analysis, based on relative EST abundance in data was performed by 454 pyrosequencing of cDNA representing mRNA of the melon fruit. Fourteen double strand cDNA samples obtained from two genotypes, two fruit tissues (flesh and rind) and four developmental stages were sequenced. GS FLX pyrosequencing (Roche/454 Life Sciences) of non-normalized and purified cDNA samples yielded 1,150,657 expressed sequence tags, that assembled into 67,477 unigenes (32,357 singletons and 35,120 contigs). Analysis of the data obtained against the Cucurbit Genomics Database [Hypertext Transfer Protocol://World Wide Web (dot) icugi (dot) org/] confirmed the accuracy of the sequencing and assembly. Expression patterns of selected genes fitted well their qRT-PCR data.

Example 2

Production of Sorghum Transcriptom and High Throughput Correlation Analysis with Yield, Nue, and Abst Related Parameters Measured in Fields Using 44K Sorguhm Oligonucleotide Micro-Arrays

In order to produce a high throughput correlation analysis between plant phenotype and gene expression level, the present inventors utilized a sorghum oligonucleotide micro-array, produced by Agilent Technologies [Hypertext Transfer Protocol://World Wide Web (dot) chem. (dot) agilent (dot) com/Scripts/PDS (dot) asp?1Page=50879]. The array oligonucleotide represents about 44,000 sorghum genes and transcripts. In order to define correlations between the levels of RNA expression with ABST, yield and NUE components or vigor related parameters, various plant characteristics of 17 different sorghum hybrids were analyzed. Among them, 10 hybrids encompassing the observed variance were selected for RNA expression analysis. The correlation between the RNA levels and the characterized parameters was analyzed using Pearson correlation test [Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot) com/hyperstat/A34739 (dot) html].

Correlation of Sorghum Varieties Across Ecotypes Grown Under Regular Growth Conditions, Severe Drought Conditions and Low Nitrogen Conditions

Experimental Procedures

17 Sorghum varieties were grown in 3 repetitive plots, in field. Briefly, the growing protocol was as follows:

1. Regular growth conditions: sorghum plants were grown in the field using commercial fertilization and irrigation protocols, which include 370 m³water per dunam (1000 m²) per entire growth period and fertilization of 14 units of URAN® 21% (Nitrogen Fertilizer Solution; PCS Sales, Northbrook, Ill., USA) (normal growth conditions).

2. Drought conditions: sorghum seeds were sown in soil and grown under normal condition until around 35 days from sowing, around stage V8 (eight green leaves are fully expanded, booting not started yet). At this point, irrigation was stopped, and severe drought stress was developed.

3. Low Nitrogen fertilization conditions: sorghum plants were fertilized with 50% less amount of nitrogen in the field than the amount of nitrogen applied in the regular growth treatment. All the fertilizer was applied before flowering.

Analyzed Sorghum tissues—All 10 selected Sorghum hybrids were sample per each treatment. Tissues [Flag leaf, Flower meristem and Flower] from plants growing under normal conditions, severe drought stress and low nitrogen conditions were sampled and RNA was extracted as described above. Each micro-array expression information tissue type has received a Set ID as summarized in Table 1 below.

TABLE 1

Sorghum transcriptom expression sets in field experiments

Expression Set
Set ID

Sorghum field/flag leaf/Drought
1

Sorghum field/flag leaf/Low N
2

Sorghum field/flag leaf/Normal
3

Sorghum field/flower meristem/Drought
4

Sorghum field/flower meristem/Low N
5

Sorghum field/flower meristem/Normal
6

Sorghum field/flower/Drought
7

Sorghum field/flower/Low N
8

Sorghum field/flower/Normal
9

Table 1: Provided are the sorghum transcriptom expression sets. Flag leaf = the leaf below the flower; Flower meristem = Apical meristem following panicle initiation; Flower = the flower at the anthesis day.

The following parameters were collected using digital imaging system:

Average grain area (cm²)—At the end of the growing period the grains were separated from the Plant ‘Head’. A sample of ˜200 grains were weighted, photographed and images were processed using the below described image processing system. The grain area was measured from those images and was divided by the number of grains.

Upper and lower ratio average of grain area, width, length, diameter and perimeter—Grain projection of area, width, length, diameter and perimeter were extracted from the digital images using open source package imagej (nih). Seed data was analyzed in plot average levels as follows:

Average of all seeds.

Average of upper 20% fraction—contained upper 20% fraction of seeds.

Average of lower 20% fraction—contained lower 20% fraction of seeds.

Further on, ratio between each fraction and the plot average was calculated for each of the data parameters.

At the end of the growing period 5 ‘Heads’ were, photographed and images were processed using the below described image processing system.

Average grain length (cm)—At the end of the growing period the grains were separated from the Plant ‘Head’. A sample of ˜200 grains were weighted, photographed and images were processed using the below described image processing system. The sum of grain lengths (longest axis) was measured from those images and was divided by the number of grains.

Head average area (cm²)—At the end of the growing period 5 ‘Heads’ were photographed and images were processed using the below described image processing system. The ‘Head’ area was measured from those images and was divided by the number of ‘Heads’.

Head average length (cm)—At the end of the growing period 5 ‘Heads’ were photographed and images were processed using the below described image processing system. The ‘Head’ length (longest axis) was measured from those images and was divided by the number of ‘Heads’.

Head average width (cm)—At the end of the growing period 5 ‘Heads’ were photographed and images were processed using the below described image processing system. The ‘Head’ width was measured from those images and was divided by the number of ‘Heads’.

Head average perimeter (cm)—At the end of the growing period 5 ‘Heads’ were photographed and images were processed using the below described image processing system. The ‘Head’ perimeter was measured from those images and was divided by the number of ‘Heads’.

An image processing system was used, which consists of a personal desktop computer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ 1.37, Java based image processing software, which was developed at the U.S. National Institutes of Health and is freely available on the internet at Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/. Images were captured in resolution of 10 Mega Pixels (3888×2592 pixels) and stored in a low compression JPEG (Joint Photographic Experts Group standard) format. Next, image processing output data for seed area and seed length was saved to text files and analyzed using the JMP statistical analysis software (SAS institute).

Additional parameters were collected either by sampling 5 plants per plot or by measuring the parameter across all the plants within the plot.

Total seed weight per head (Grain yield) (gr.)—At the end of the experiment (plant ‘Heads’) heads from plots within blocks A-C were collected. 5 heads were separately threshed and grains were weighted, all additional heads were threshed together and weighted as well. The average grain weight per head was calculated by dividing the total grain weight by number of total heads per plot (based on plot). In case of 5 heads, the total grains weight of 5 heads was divided by 5.

FW (fresh weight) head per plant (gr.)—At the end of the experiment (when heads were harvested) total heads and 5 selected heads per plots within blocks A-C were collected separately. The heads (total and 5) were weighted (gr.) separately, and the average fresh weight per plant was calculated for total (FW Head/Plant gr. based on plot) and for 5 heads (FW Head/Plant gr. based on 5 plants).

Plant height—Plants were characterized for height during growing period at 5 time points. In each measure, plants were measured for their height using a measuring tape. Height was measured from ground level to top of the longest leaf.

Plant leaf number—Plants were characterized for leaf number during growing period at 5 time points. In each measure, plants were measured for their leaf number by counting all the leaves of 3 selected plants per plot.

SPAD [SPAD unit]—Chlorophyll content was determined using a Minolta SPAD 502 chlorophyll meter and measurement was performed 64 days post sowing. SPAD meter readings were done on young fully developed leaves. Three measurements per leaf were taken per plot.

Vegetative fresh weight and Heads—At the end of the experiment (when inflorescence were dry) all inflorescence and vegetative material from plots within blocks A-C were collected. The biomass and heads weight of each plot was separated, measured and divided by the number of heads.

Plant biomass (Fresh weight)—At the end of the experiment (when inflorescence were dry) the vegetative material from plots within blocks A-C were collected. The plants biomass without the inflorescence were measured and divided by the number of plants.

FW (fresh weight) heads/(FW Heads+FW Plants)—The total fresh weight of heads and their respective plant biomass were measured at the harvest day. The heads weight was divided by the sum of weights of heads and plants.

Dry weight=total weight of the vegetative portion above ground (excluding roots) after drying at 70° C. in oven for 48 hours;

Harvest Index (HI) (Sorghum)—The harvest index was calculated using Formula V.

Harvest Index (Sorghum)=Average grain dry weight per Head/(Average vegetative dry weight per Head+Average Head dry weight) Formula V

Data parameters collected are summarized in Table 2, herein below

TABLE 2

Sorghum correlated parameters (vectors)

Correlated parameter with
Correlation ID

Average Grain Area (cm²), Drought
1

Average Grain Area (cm²), Low Nitrogen (N)
2

Average Grain Area (cm²), Normal
3

FW - Head/Plant (gr.) (based on plot), Drought
4

FW - Head/Plant (gr.) (based on plot), Low N
5

FW - Head/Plant (gr.) (based on plot), Normal
6

FW - Head/Plant (gr.) (based on 5 plants), Low N
7

FW - Head/Plant (gr.) (based on 5 plants), Normal
8

FW Heads/(FW Heads + FW Plants) (all plot), Drought
9

FW Heads/(FW Heads + FW Plants) (all plot), Low N
10

FW Heads/(FW Heads + FW Plants) (all plot), Normal
11

FW/Plant gr (based on plot), Drought
12

FW/Plant gr (based on plot), Low N
13

FW/Plant gr (based on plot), Normal
14

Final Plant Height (cm), Drought
15

Final Plant Height (cm), Low N
16

Final Plant Height (cm), Normal
17

Head Average Area (cm²), Drought
18

Head Average Area (cm²), Low N
19

Head Average Area (cm²), Normal
20

Head Average Length (cm), Drought
21

Head Average Length (cm), Low N
22

Head Average Length (cm), Normal
23

Head Average Perimeter (cm), Drought
24

Head Average Perimeter (cm), Low N
25

Head Average Perimeter (cm), Normal
26

Head Average Width (cm), Drought
27

Head Average Width (cm), Low N
28

Head Average Width (cm), Normal
29

Leaf 64 DPS (Days Post Sowing), Drought (SPAD unit)
30

Leaf SPAD 64 DPS (Days Post Sowing), Low N
31

(SPAD unit)

Leaf SPAD 64 DPS (Days Post Sowing), Normal
32

(SPAD unit)

Lower Ratio Average Grain Area, Low N
33

Lower Ratio Average Grain Area, Normal
34

Lower Ratio Average Grain Length, Low N
35

Lower Ratio Average Grain Length, Normal
36

Lower Ratio Average Grain Perimeter, Low N
37

Lower Ratio Average Grain Perimeter, Normal
38

Lower Ratio Average Grain Width, Low N
39

Lower Ratio Average Grain Width, Normal
40

Total grain weight/Head (based on plot) (gr.), Low N
41

Total grain weight/Head (based on 5 heads) (gr.),
42

Low N

Total grain weight/Head (based on 5 heads) (gr.),
43

Normal

Total grain weight/Head (based on plot) (gr.), Normal
44

Total grain weight/Head (based on plot) (gr.) Drought
45

Upper Ratio Average Grain Area, Drought
46

Upper Ratio Average Grain Area, Low N
47

Upper Ratio Average Grain Area, Normal
48

[Grain Yield + plant biomass/SPAD 64 DPS],
49

Normal

[Grain Yield + plant biomass/SPAD 64 DPS],
50

Low N

[Grain yield/SPAD 64 DPS], Low N
51

[Grain yield/SPAD 64 DPS], Normal
52

[Plant biomass (FW)/SPAD 64 DPS], Drought
53

[Plant biomass (FW)/SPAD 64 DPS], Low N
54

[Plant biomass (FW)/SPAD 64 DPS], Normal
55

Table 2. Provided are the Sorghum correlated parameters (vectors). “gr.” = grams; “SPAD” = chlorophyll levels; “FW” = Plant Fresh weight; “DW” = Plant Dry weight; “normal” = standard growth conditions; “DPS” = days post-sowing; “Low N” = Low Nitrogen conditions; “Head FW” = fresh weight of the harvested heads was divided by the number of heads that were phenotyped; “Lower Ratio Average Grain Area” = grain area of the lower fraction of grains.

Experimental Results

17 different sorghum hybrids were grown and characterized for different parameters (Table 2). The average for each of the measured parameter was calculated using the JMP software (Tables 3-8) and a subsequent correlation analysis was performed (Table 9). Results were then integrated to the database.

TABLE 3

Measured parameters in Sorghum accessions under normal conditions

Line/

Corr. ID
3
6
8
11
14
17
20
23
26
29
32

Line-1
0.10
175.15
406.50
0.51
162.56
95.25
120.14
25.58
61.22
5.97
43.01

Line-2
0.11
223.49
518.00
0.51
212.59
79.20
167.60
26.84
67.90
7.92
.

Line-3
0.13
56.40
148.00
0.12
334.83
197.85
85.14
21.02
56.26
4.87
43.26

Line-4
0.13
111.62
423.00
0.26
313.46
234.20
157.26
26.84
65.38
7.43
44.74

Line-5
0.14
67.34
92.00
0.12
462.28
189.40
104.00
23.14
67.46
5.58
45.76

Line-6
0.14
66.90
101.33
0.18
318.26
194.67
102.48
21.82
67.46
5.88
41.61

Line-7
0.11
126.18
423.50
0.46
151.13
117.25
168.54
31.33
74.35
6.78
45.21

Line-8
0.11
107.74
386.50
0.43
137.60
92.80
109.32
23.18
56.16
5.99
45.14

Line-9
0.10
123.86
409.50
0.42
167.98
112.65
135.13
25.70
61.64
6.62
43.03

Line-10
0.12
102.75
328.95
0.44
128.97
97.50
169.03
28.82
71.40
7.42
45.59

Line-11
0.12
82.33
391.00
0.46
97.62
98.00
156.10
28.13
68.56
6.98
44.83

Line-12
0.11
77.59
435.75
0.46
99.32
100.00
112.14
22.97
56.44
6.19
45.33

Line-13
0.12
91.17
429.50
0.45
112.24
105.60
154.74
28.09
67.79
7.02
46.54

Line-14
0.11
150.44
441.00
0.51
157.42
151.15
171.70
30.00
71.54
7.18
43.99

Line-15
0.10
109.10
415.75
0.46
130.55
117.10
168.51
30.54
78.94
7.00
45.09

Line-16
0.11
107.58
429.50
0.44
135.66
124.45
162.51
27.17
67.03
7.39
45.14

Line-17
0.11
130.88
428.50
0.39
209.21
126.50
170.46
29.26
74.11
7.35
43.13

Table 3: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under normal conditions. Growth conditions are specified in the experimental procedure section.

TABLE 4

Additional measured parameters in Sorghum accessions under normal growth conditions

Line/

Corr. ID
34
36
38
40
43
44
48
49
52
55

Line-1
0.83
0.91
0.91
0.91
47.40
31.12
1.22
4.50
3.78
0.72

Line-2
0.74
0.88
0.87
0.83
46.30
26.35
1.30
8.17
7.74
0.43

Line-3
0.78
0.92
0.91
0.85
28.37
18.72
1.13
7.87
7.01
0.86

Line-4
0.80
0.91
0.95
0.87
70.40
38.38
1.14
10.68
10.10
0.58

Line-5
0.70
0.89
0.90
0.79
32.15
26.67
1.16
8.34
7.65
0.69

Line-6
0.70
0.88
0.91
0.80
49.23
28.84
1.15
4.40
3.34
1.05

Line-7
0.83
0.91
0.91
0.90
63.45
47.67
1.19
3.74
3.05
0.69

Line-8
0.81
0.90
0.91
0.89
44.45
31.00
1.23
4.83
3.90
0.93

Line-9
0.84
0.92
0.92
0.91
56.65
39.99
1.25
3.67
2.83
0.84

Line-10
0.79
0.92
0.93
0.85
60.00
38.36
1.24
2.89
2.18
0.72

Line-11
0.77
0.89
0.91
0.86
45.45
32.10
1.32
2.91
2.19
0.72

Line-12
0.80
0.91
0.92
0.88
58.19
32.69
1.22
3.12
2.41
0.70

Line-13
0.81
0.91
0.90
0.90
70.60
32.79
1.18
4.75
3.58
1.17

Line-14
0.82
0.91
0.91
0.90
70.10
51.53
1.18
3.69
2.90
0.79

Line-15
0.81
0.90
0.90
0.91
53.95
35.71
1.22
3.85
3.01
0.85

Line-16
0.82
0.90
0.91
0.90
59.87
38.31
1.25
5.84
4.85
0.98

Line-17
0.82
0.91
0.91
0.90
52.65
42.44
1.22

Table 4: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under normal conditions. Growth conditions are specifiedin the experimental procedure section.

Table 5

Measured parameters in Sorghum accessions under low nitrogen conditions

Line/

Corr. ID
2
5
7
10
13
16
19
22
25
28
31

Line-1
0.11
214.78
388
0.51
204.78
104
96.24
23.22
56.32
5.26
38.33

Line-2
0.11
205.05
428.67
0.51
199.64
80.93
214.72
25.58
79.20
10.41
38.98

Line-3
0.14
73.49
297.67
0.17
340.51
204.73
98.59
20.93
53.25
5.93
42.33

Line-4
0.12
122.96
280.00
0.39
240.60
125.40
182.83
28.43
76.21
8.25
40.90

Line-5
0.14
153.07
208.33
0.21
537.78
225.40
119.64
24.32
67.27
6.19
43.15

Line-6
0.13
93.23
303.67
0.19
359.40
208.07
110.19
22.63
59.49
6.12
39.85

Line-7
0.12
134.11
436.00
0.48
149.20
121.40
172.36
32.11
79.28
6.80
42.68

Line-8
0.12
77.43
376.33
0.37
129.06
100.27
84.81
20.38
51.52
5.25
43.31

Line-9
0.12
129.63
474.67
0.42
178.71
121.13
156.25
26.69
69.88
7.52
39.01

Line-10
0.13
99.83
437.67
0.44
124.27
94.53
136.71
26.31
66.17
6.59
42.71

Line-11
0.13
76.95
383.00
0.43
101.33
110.00
137.70
25.43
67.37
6.85
40.08

Line-12
0.12
84.25
375.00
0.39
132.12
115.07
96.54
23.11
57.90
5.32
43.98

Line-13
0.12
92.24
425.00
0.44
117.90
104.73
158.19
27.87
70.61
7.25
45.44

Line-14
0.11
138.83
434.00
0.44
176.99
173.67
163.95
28.88
73.76
7.19
44.75

Line-15
0.11
113.32
408.67
0.44
143.67
115.60
138.39
27.64
66.87
6.27
42.58

Line-16
0.12
95.50
378.50
0.43
126.98
138.80
135.46
25.52
65.40
6.57
43.81

Line-17
0.11
129.49
432.00
0.42
180.45
144.40
165.64
30.33
75.97
6.82
46.73

Table 5: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under low nitrogen conditions. Growth conditions are specified in the experimental procedure section.

TABLE 6

Additional measured parameters in Sorghum accessions under low nitrogen growth conditions

Line/

Corr. ID
33
35
37
39
41
42
47
50
51
54

Line-1
0.82
0.91
0.90
0.90
25.95
50.27
1.18
6.02
0.68
5.34

Line-2
0.77
0.90
0.88
0.85
30.57
50.93
1.31
5.91
0.78
5.12

Line-3
0.81
0.92
0.92
0.89
19.37
36.13
1.11
8.50
0.46
8.05

Line-4
0.79
0.90
0.90
0.88
35.62
73.10
1.21
6.75
0.87
5.88

Line-5
0.78
0.91
0.92
0.86
25.18
37.87
1.19
13.05
0.58
12.46

Line-6
0.80
0.93
0.92
0.87
22.18
36.40
1.18
9.58
0.56
9.02

Line-7
0.83
0.92
0.92
0.91
49.96
71.67
1.16
4.67
1.17
3.50

Line-8
0.79
0.89
0.89
0.89
27.48
35.00
1.23
3.61
0.63
2.98

Line-9
0.81
0.90
0.90
0.90
51.12
76.73
1.17
5.89
1.31
4.58

Line-10
0.77
0.91
0.91
0.86
36.84
57.58
1.22
3.77
0.86
2.91

Line-11
0.74
0.89
0.89
0.84
29.45
42.93
1.24
3.26
0.73
2.53

Line-12
0.80
0.90
0.90
0.90
26.70
36.47
1.19
3.61
0.61
3.00

Line-13
0.79
0.89
0.90
0.89
29.42
68.60
1.23
3.24
0.65
2.60

Line-14
0.82
0.91
0.91
0.91
51.12
71.80
1.16
5.10
1.14
3.96

Line-15
0.80
0.89
0.89
0.90
37.04
49.27
1.34
4.25
0.87
3.38

Line-16
0.81
0.89
0.90
0.90
39.85
43.87
1.21
3.81
0.91
2.90

Line-17
0.81
0.90
0.90
0.90
41.78
52.07
1.21
4.76
0.89
3.86

Table 6: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under low nitrogen conditions. Growth conditions are specified in the experimental procedure section.

TABLE 7

Measured parameters in Sorghum accessions under drought conditions

Line/Corr. ID
1
4
9
12
15
18
21
24
27

Line-1
0.10
154.90
0.42
207.99
89.40
83.14
21.63
52.78
4.83

Line-2
0.11
122.02
0.47
138.02
75.73
107.79
21.94
64.49
6.31

Line-3
0.11
130.51
0.42
255.41
92.10
88.68
21.57
56.59
5.16

Line-4
0.09
241.11
0.37
402.22
94.30
135.91
22.01
64.37
7.78

Line-5
0.09
69.03
0.23
233.55
150.80
90.76
20.99
53.21
5.28

Line-6
0.11
186.41
0.31
391.75
110.73
123.95
28.60
71.66
5.49

Line-7

62.11
0.41
89.31
99.20
86.06
21.35
55.61
5.04

Line-8

39.02
0.44
50.61
84.00
85.20
20.81
52.96
5.07

Line-9

58.94
0.40
87.02
99.00
113.10
24.68
69.83
5.77

Line-10

76.37
0.44
120.43
92.20
100.79
24.28
65.14
5.37

Line-11

33.47
0.47
37.21
81.93
80.41
21.95
55.27
4.66

Line-12

42.20
0.47
48.18
98.80
126.89
24.98
69.06
6.35

Line-13

41.53
0.48
44.20
86.47
86.41
19.49
53.32
5.58

Line-14

131.67
0.35
231.60
99.60
92.29
20.42
56.29
5.76

Line-15

60.84
0.35
116.01
83.00
77.89
16.81
49.12
5.86

Line-16

44.33
0.23
123.08
83.53
76.93
18.88
51.88
5.10

Line-17

185.44
0.33
342.50
92.30

Table 7: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under drought conditions. Growth conditions are specified in the experimental procedure section.

TABLE 8

Additional measured parameters in Sorghum accessions

under drought growth conditions

Correlation ID

Line
30
45
46
53

Line-1
40.58
22.11
1.31
5.13

Line-2
40.88
16.77
1.19
3.38

Line-3
45.01
9.19
1.29
5.67

Line-4
42.30
104.44
1.46
9.51

Line-5
45.24
3.24
1.21
5.16

Line-6
40.56
22.00
1.21
9.66

Line-7
44.80
9.97

1.99

Line-8
45.07
18.58

1.12

Line-9
40.65
29.27

2.14

Line-10
45.43
10.45

2.65

Line-11
42.58
14.77

0.87

Line-12
44.18
12.86

1.09

Line-13
44.60
18.24

0.99

Line-14
42.41
11.60

5.46

Line-15
43.25
18.65

2.68

Line-16
40.30
16.36

3.05

Line-17
40.75

8.40

Table 8: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under drought conditions. Growth conditions are specified in the experimental procedure section.

TABLE 9

Correlation between the expression level of selected LAB genes of some embodiments of the

invention in various tissues and the phenotypic performance under low nitrogen, normal or

drought stress conditions across Sorghum accessions

Gene

Exp.
Corr.
Gene

Exp.
Corr.

Name
R
P value
set
Set ID
Name
R
P value
set
Set ID

LAB740
0.76
1.14E−02
3
48
LAB741
0.70
2.31E−02
8
16

LAB741
0.75
2.02E−02
3
52
LAB741
0.76
1.76E−02
3
49

LAB742
0.87
1.14E−03
5
2
LAB744
0.86
1.53E−03
6
17

LAB744
0.72
1.93E−02
6
44
LAB744
0.97
6.12E−06
4
53

LAB744
0.91
3.04E−04
4
4
LAB744
0.97
5.61E−06
4
12

LAB744
0.81
8.34E−03
7
18
LAB744
0.90
8.98E−04
7
27

LAB744
0.73
2.68E−02
7
24
LAB745
0.78
1.39E−02
4
18

LAB745
0.72
2.93E−02
4
24
LAB745
0.82
7.22E−03
4
21

LAB746
0.74
1.42E−02
9
48
LAB746
0.78
8.00E−03
9
3

LAB746
0.95
3.22E−05
2
47
LAB746
0.93
9.99E−05
8
47

LAB746
0.89
6.63E−04
5
47
LAB748
0.82
3.53E−03
6
44

LAB748
0.74
1.46E−02
6
36
LAB748
0.70
2.39E−02
9
3

LAB748
0.71
2.24E−02
4
15
LAB748
0.75
1.16E−02
5
41

LAB748
0.76
1.08E−02
5
51
LAB748
0.74
1.47E−02
5
16

LAB748
0.72
1.79E−02
1
53
LAB748
0.73
1.76E−02
1
12

LAB749
0.80
4.97E−03
6
52
LAB749
0.79
7.07E−03
6
49

LAB750
0.80
5.37E−03
6
48
LAB750
0.74
1.44E−02
6
3

LAB750
0.77
9.90E−03
2
47
LAB750
0.79
6.31E−03
5
2

LAB751
0.82
3.77E−03
6
17
LAB751
0.73
1.68E−02
9
17

LAB751
0.72
1.82E−02
2
33
LAB751
0.77
8.76E−03
2
41

LAB751
0.76
1.04E−02
2
35
LAB751
0.80
5.82E−03
2
51

LAB751
0.83
2.68E−03
2
37
LAB751
0.74
2.35E−02
7
18

LAB751
0.71
3.09E−02
7
24
LAB752
0.73
1.68E−02
6
17

LAB752
0.86
1.50E−03
6
44
LAB752
0.78
7.54E−03
9
8

LAB752
0.88
6.79E−04
4
53
LAB752
0.74
1.42E−02
4
4

LAB752
0.89
6.10E−04
4
12
LAB752
0.71
2.10E−02
5
50

LAB752
0.71
2.04E−02
5
13
LAB753
0.82
3.94E−03
6
17

LAB753
0.75
1.29E−02
9
23
LAB753
0.75
1.34E−02
2
42

LAB753
0.72
1.86E−02
8
37
LAB753
0.71
2.22E−02
8
16

LAB754
0.77
9.07E−03
6
52
LAB754
0.77
8.88E−03
6
49

LAB754
0.77
9.14E−03
2
47
LAB754
0.75
1.20E−02
2
28

LAB754
0.72
1.89E−02
8
41
LAB754
0.82
4.01E−03
8
42

LAB754
0.74
1.46E−02
8
51
LAB755
0.72
1.96E−02
9
40

LAB755
0.78
1.25E−02
3
55
LAB757
0.82
3.87E−03
9
48

LAB757
0.73
1.68E−02
9
3
LAB757
0.71
3.29E−02
4
27

LAB757
0.84
2.21E−03
4
53
LAB757
0.76
1.14E−02
4
4

LAB757
0.86
1.58E−03
4
12
LAB757
0.85
1.64E−03
5
28

LAB758
0.71
2.27E−02
6
17
LAB758
0.71
2.19E−02
6
11

LAB758
0.73
2.70E−02
9
49
LAB758
0.80
5.87E−03
4
53

LAB758
0.78
7.49E−03
4
4
LAB758
0.81
4.67E−03
4
12

LAB758
0.74
1.45E−02
5
13
LAB759
0.90
4.66E−04
6
17

LAB759
0.83
2.71E−03
6
44
LAB759
0.92
1.42E−04
4
53

LAB759
0.87
1.06E−03
4
4
LAB759
0.92
1.43E−04
4
12

LAB759
0.76
1.65E−02
7
18
LAB759
0.71
3.27E−02
7
21

LAB760
0.75
1.23E−02
6
48
LAB760
0.79
6.38E−03
8
5

LAB760
0.77
9.22E−03
8
50
LAB760
0.80
5.30E−03
8
54

LAB760
0.72
1.84E−02
8
13
LAB760
0.74
1.49E−02
3
3

LAB760
0.85
4.07E−03
7
27
LAB760
0.71
3.12E−02
7
24

LAB761
0.73
1.56E−02
2
41
LAB761
0.70
2.30E−02
2
51

LAB761
0.78
8.11E−03
2
16
LAB761
0.76
9.97E−03
3
17

LAB761
0.71
2.25E−02
3
44
LAB761
0.75
1.20E−02
7
4

LAB762
0.78
8.29E−03
8
35
LAB762
0.74
2.32E−02
7
18

LAB762
0.74
2.18E−02
7
24
LAB762
0.86
3.11E−03
7
21

LAB763
0.75
1.30E−02
6
17
LAB763
0.79
6.61E−03
2
47

LAB763
0.85
1.72E−03
4
53
LAB763
0.74
1.45E−02
4
4

LAB763
0.86
1.43E−03
4
12
LAB763
0.71
2.18E−02
5
5

LAB763
0.77
8.52E−03
5
50
LAB763
0.73
1.57E−02
5
54

LAB763
0.85
1.93E−03
5
13
LAB763
0.80
1.04E−02
3
52

LAB763
0.80
9.24E−03
3
49
LAB763
0.72
1.87E−02
7
30

LAB764
0.71
2.14E−02
2
42
LAB764
0.76
1.07E−02
2
37

LAB764
0.75
1.22E−02
1
15
LAB765
0.74
2.24E−02
9
52

LAB765
0.75
1.93E−02
9
49
LAB765
0.71
3.17E−02
4
27

LAB765
0.71
3.33E−02
4
24
LAB765
0.79
6.67E−03
8
47

LAB765
0.74
1.47E−02
1
53
LAB765
0.74
1.47E−02
1
12

LAB768
0.72
1.98E−02
6
48
LAB768
0.80
5.64E−03
6
3

LAB768
0.76
1.14E−02
5
2
LAB768
0.78
7.25E−03
7
9

LAB769
0.76
1.08E−02
9
17
LAB769
0.70
2.35E−02
5
16

LAB769
0.79
1.22E−02
3
52
LAB769
0.74
1.41E−02
3
48

LAB769
0.74
2.14E−02
3
49
LAB769
0.70
3.52E−02
7
18

LAB769
0.86
3.03E−03
7
27
LAB770
0.71
3.13E−02
4
45

LAB770
0.75
1.96E−02
3
52
LAB770
0.80
1.03E−02
3
49

LAB770
0.76
1.76E−02
7
45
LAB771
0.73
1.73E−02
6
17

LAB771
0.78
7.16E−03
6
44
LAB771
0.78
7.98E−03
2
47

LAB771
0.84
2.54E−03
4
53
LAB771
0.78
8.35E−03
4
4

LAB771
0.85
1.94E−03
4
12
LAB771
0.79
6.53E−03
5
41

LAB771
0.79
6.27E−03
5
51
LAB771
0.74
2.30E−02
3
52

LAB771
0.75
2.01E−02
3
49
LAB772
0.81
4.32E−03
6
17

LAB772
0.78
7.81E−03
6
44
LAB772
0.70
2.29E−02
2
33

LAB772
0.90
4.60E−04
2
41
LAB772
0.89
6.39E−04
2
22

LAB772
0.74
1.43E−02
2
42
LAB772
0.86
1.41E−03
2
51

LAB772
0.87
9.89E−04
2
16
LAB772
0.83
2.66E−03
4
53

LAB772
0.80
5.63E−03
4
4
LAB772
0.85
2.04E−03
4
12

LAB772
0.77
9.58E−03
5
41
LAB772
0.71
2.19E−02
5
13

LAB772
0.74
1.38E−02
5
51
LAB773
0.77
9.30E−03
6
17

LAB773
0.71
2.15E−02
9
8
LAB773
0.84
2.54E−03
4
53

LAB773
0.81
4.69E−03
4
4
LAB773
0.85
1.88E−03
4
12

LAB773
0.72
1.92E−02
5
13
LAB774
0.74
1.52E−02
9
17

LAB774
0.72
1.98E−02
2
47
LAB774
0.81
4.73E−03
2
28

LAB774
0.82
7.26E−03
4
18
LAB774
0.73
2.66E−02
4
24

LAB775
0.74
1.54E−02
6
17
LAB775
0.78
7.21E−03
6
44

LAB775
0.78
7.93E−03
2
37
LAB775
0.98
8.19E−07
4
53

LAB775
0.96
1.13E−05
4
4
LAB775
0.98
3.93E−07
4
12

LAB775
0.73
1.72E−02
5
5
LAB775
0.79
6.49E−03
5
50

LAB775
0.73
1.61E−02
5
54
LAB775
0.84
2.53E−03
5
13

LAB775
0.91
2.28E−04
7
30
LAB775
0.91
3.11E−04
1
15

LAB776
0.75
1.31E−02
6
3
LAB776
0.71
2.03E−02
9
44

LAB776
0.75
1.28E−02
4
30
LAB776
0.76
1.06E−02
5
2

LAB777
0.80
5.08E−03
2
41
LAB777
0.79
6.63E−03
2
51

LAB777
0.77
9.42E−03
2
37
LAB777
0.85
1.68E−03
5
41

LAB777
0.84
2.11E−03
5
51
LAB777
0.71
2.04E−02
5
16

LAB777
0.77
8.69E−03
3
44
LAB778
0.94
7.00E−05
9
3

LAB778
0.78
7.49E−03
8
2
LAB778
0.71
3.18E−02
3
55

LAB779
0.75
1.93E−02
7
45
LAB779
0.77
1.53E−02
1
27

LAB780
0.74
1.39E−02
6
29
LAB780
0.77
8.58E−03
6
17

LAB780
0.78
7.79E−03
6
20
LAB780
0.83
3.10E−03
4
53

LAB780
0.84
2.13E−03
4
4
LAB780
0.82
3.35E−03
4
12

LAB780
0.71
2.10E−02
8
37
LAB780
0.74
1.40E−02
3
3

LAB780
0.80
1.01E−02
7
27
LAB781
0.81
4.08E−03
6
3

LAB781
0.75
1.32E−02
5
2
LAB781
0.90
4.08E−04
1
53

LAB781
0.79
6.44E−03
1
4
LAB781
0.90
3.55E−04
1
12

LAB782
0.72
1.91E−02
9
6
LAB783
0.88
8.55E−04
6
17

LAB783
0.78
7.57E−03
6
44
LAB783
0.78
7.96E−03
4
53

LAB783
0.72
1.86E−02
4
4
LAB783
0.79
7.14E−03
4
12

LAB784
0.88
8.40E−04
6
52
LAB784
0.87
9.73E−04
6
49

LAB784
0.88
8.07E−04
9
48
LAB784
0.72
1.82E−02
9
3

LAB784
0.82
3.79E−03
5
2
LAB784
0.87
1.19E−03
3
48

LAB785
0.75
1.23E−02
6
17
LAB785
0.71
2.03E−02
2
7

LAB785
0.74
1.39E−02
4
53
LAB785
0.74
1.50E−02
4
4

LAB785
0.75
1.29E−02
4
12
LAB785
0.83
5.94E−03
3
52

LAB785
0.84
2.45E−03
3
6
LAB785
0.79
1.05E−02
3
49

LAB785
0.78
8.32E−03
3
8
LAB786
0.83
2.73E−03
6
3

LAB786
0.71
2.14E−02
9
8
LAB787
0.83
3.19E−03
6
52

LAB787
0.88
8.30E−04
6
6
LAB787
0.83
2.90E−03
6
14

LAB787
0.79
6.02E−03
6
49
LAB787
0.78
7.78E−03
2
10

LAB788
0.74
1.43E−02
6
48
LAB788
0.82
3.40E−03
6
3

LAB788
0.74
1.52E−02
4
30
LAB788
0.78
7.80E−03
5
2

LAB788
0.80
5.56E−03
1
30
LAB789
0.74
1.43E−02
6
11

LAB789
0.73
2.50E−02
9
52
LAB789
0.75
2.02E−02
9
49

LAB789
0.89
5.77E−04
4
53
LAB789
0.87
1.11E−03
4
4

LAB789
0.88
7.77E−04
4
12
LAB789
0.80
5.83E−03
5
5

LAB789
0.85
1.90E−03
5
50
LAB789
0.81
4.25E−03
5
54

LAB789
0.89
5.59E−04
5
13
LAB790
0.70
2.30E−02
6
52

LAB790
0.72
1.79E−02
6
49
LAB790
0.71
2.26E−02
2
47

LAB790
0.73
2.70E−02
4
21
LAB791
0.78
7.61E−03
8
10

LAB791
0.79
6.94E−03
8
42
LAB792
0.72
1.89E−02
5
2

LAB793
0.79
6.56E−03
5
2
LAB794
0.75
1.29E−02
6
3

LAB794
0.81
4.14E−03
2
41
LAB794
0.80
5.35E−03
2
22

LAB794
0.74
1.48E−02
2
42
LAB794
0.76
1.02E−02
2
51

LAB794
0.71
2.03E−02
2
16
LAB794
0.75
1.22E−02
8
16

LAB794
0.79
6.69E−03
5
2
LAB794
0.83
5.96E−03
3
52

LAB794
0.84
2.29E−03
3
6
LAB794
0.83
2.94E−03
3
14

LAB794
0.79
1.12E−02
3
49
LAB794
0.76
1.15E−02
1
53

LAB794
0.79
6.99E−03
1
4
LAB794
0.75
1.30E−02
1
12

LAB796
0.80
5.65E−03
6
48
LAB797
0.85
1.65E−03
6
17

LAB797
0.82
4.04E−03
6
44
LAB797
0.82
3.33E−03
4
53

LAB797
0.77
8.61E−03
4
4
LAB797
0.83
2.89E−03
4
12

LAB797
0.72
1.79E−02
5
5
LAB797
0.79
6.57E−03
5
50

LAB797
0.71
2.20E−02
5
54
LAB797
0.79
6.65E−03
5
13

LAB797
0.71
3.38E−02
3
55
LAB798
0.89
6.03E−04
6
17

LAB798
0.70
2.33E−02
6
40
LAB798
0.81
4.62E−03
6
44

LAB798
0.70
2.31E−02
6
36
LAB798
0.71
2.19E−02
6
34

LAB798
0.85
2.04E−03
4
53
LAB798
0.85
1.92E−03
4
12

LAB798
0.74
1.42E−02
5
16
LAB798
0.76
1.81E−02
3
52

LAB798
0.77
9.69E−03
3
6
LAB798
0.74
1.41E−02
3
14

LAB798
0.77
1.45E−02
3
49
LAB799
0.89
4.88E−04
6
17

LAB799
0.86
1.48E−03
6
44
LAB799
0.87
9.38E−04
4
53

LAB799
0.89
5.21E−04
4
4
LAB799
0.88
8.64E−04
4
12

LAB799
0.74
1.53E−02
5
5
LAB799
0.80
5.13E−03
5
50

LAB799
0.74
1.53E−02
5
54
LAB799
0.79
6.90E−03
5
13

Table 9. Correlations (R) between the genes expression levels in various tissues and the phenotypic performance. “Con. ID” - correlation set ID according to the correlated parameters Table above. “Exp. Set” - Expression set. “R” = Pearson correlation coefficient; “P” = p value.

Example 3
Production of Sorghum Transcriptom and High Throughput Correlation Analysis with Yield and Drought Related Parameters Measured in Fields Using 65K Sorguhm Oligonucleotide Micro-Arrays

In order to produce a high throughput correlation analysis between plant phenotype and gene expression level, the present inventors utilized a sorghum oligonucleotide micro-array, produced by Agilent Technologies [Hypertext Transfer Protocol://World Wide Web (dot) chem. (dot) agilent (dot) com/Scripts/PDS (dot) asp?1Page=50879]. The array oligonucleotide represents about 65,000 sorghum genes and transcripts. In order to define correlations between the levels of RNA expression with ABST, drought and yield components or vigor related parameters, various plant characteristics of 12 different sorghum hybrids were analyzed. Among them, 8 hybrids encompassing the observed variance were selected for RNA expression analysis. The correlation between the RNA levels and the characterized parameters was analyzed using Pearson correlation test [Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot) com/hyperstat/A34739 (dot) html].

Experimental Procedures

12 Sorghum varieties were grown in 6 repetitive plots, in field. Briefly, the growing protocol was as follows:

1. Regular growth conditions: sorghum plants were grown in the field using commercial fertilization and irrigation protocols, which include 452 m³water per dunam (1000 square meters) per entire growth period and fertilization of 14 units of URAN® 21% (Nitrogen Fertilizer Solution; PCS Sales, Northbrook, Ill., USA) (normal growth conditions).

2. Drought conditions: sorghum seeds were sown in soil and grown under normal condition until flowering stage (59 days from sowing), drought treatment was imposed by irrigating plants with 50% water relative to the normal treatment from this stage [309 m³water per dunam (1000 square meters) per entire growth period].

Analyzed Sorghum tissues—All 12 selected Sorghum hybrids were sample per each treatment. Tissues [Flag leaf, upper stem, lower stem, flower, grain] representing different plant characteristics, from plants growing under normal conditions and drought stress conditions were sampled and RNA was extracted as described above. Each micro-array expression information tissue type has received a Set ID as summarized in Table 10 below.

TABLE 10

Sorghum transcriptom expression sets in field experiment

Expression Set
Set ID

Sorghum/flag leaf/Drought/flowering
1

Sorghum/flag leaf/Drought/grain filling
2

Table 10: Provided are the sorghum transcriptom expression sets. Flag leaf = the leaf below the flower.

Sorghum yield components and vigor related parameters assessment—Plants were phenotyped as shown in Tables 11-12 below. The following parameters were collected using digital imaging system:

Average grain width (cm)—At the end of the growing period the grains were separated from the Plant ‘Head’. A sample of ˜200 grains were weighted, photographed and images were processed using the below described image processing system. The sum of grain width (longest axis) was measured from those images and was divided by the number of grains.

Average grain perimeter (cm)—At the end of the growing period the grains were separated from the Plant ‘Head’. A sample of ˜200 grains were weighted, photographed and images were processed using the below described image processing system. The sum of grain perimeter was measured from those images and was divided by the number of grains.

Grain circularity—At the end of the growing period the grains were separated from the Plant ‘Head’. A sample of ˜200 grains were weighted, photographed and images were processed using the below described image processing system. The circularity of the grains was calculated based on Formula VI.

Grain circularity=4×3.14(grain area/perimeter²) Formula VI:

Head average area (cm²)—At the end of the growing period 8 ‘Heads’ were photographed and images were processed using the below described image processing system. The ‘Head’ area was measured from those images and was divided by the number of ‘Heads’.

Head average length (cm)—At the end of the growing period 8 ‘Heads’ were photographed and images were processed using the below described image processing system. The ‘Head’ length (longest axis) was measured from those images and was divided by the number of ‘Heads’.

Head average width (cm)—At the end of the growing period 8 ‘Heads’ were photographed and images were processed using the below described image processing system. The ‘Head’ width (longest axis) was measured from those images and was divided by the number of ‘Heads’.

Additional parameters were collected either by sampling 8 plants per plot or by measuring the parameter across all the plants within the plot.

Head dry weight at grain filling (gr.)—5 heads per plot were collected at the grain filling stage (R2-R3) and weighted after oven dry (dry weight).

Head dry weights at harvest (gr.)—At the end of the growing period heads were collected (harvest stage), either from 8 plants per plot or from the rest of the plants in the plot. Heads were weighted after oven dry (dry weight), and average head weight per plant or per plot were calculated.

Total seed yield (gr.)—At the end of the growing period heads were collected (harvest stage). 8 heads were separately threshed and grains were weighted. The average grain weight per plant was calculated by dividing the total grain weight by the number of plants.

1000 Seeds weight [gr]—weight of 1000 seeds per plot.

Grain number (num.)—was calculated by dividing seed yield by 1000 seed weight.

Plant height (cm.)—Plants were characterized for height during growing period at 6 time points (including at harvest). In each measure, plants were measured for their height using a measuring tape. Height was measured from ground level to top of the longest leaf.

SPAD—Chlorophyll content was determined using a Minolta SPAD 502 chlorophyll meter and measurement was performed at early grain filling (SPAD_earlyGF) and late grain filling (SPAD_lateGF). SPAD meter readings were done on fully developed leaf. Three measurements per leaf were taken per plant.

Vegetative fresh and dry weight per plant (gr.)—At the end of the growing period all vegetative material (excluding roots) from plots were collected and weighted before (fresh weight) and after (dry weight) oven dry. The biomass per plant was calculated by dividing total biomass by the number of plants.

Relative water content (R WC, %)—at grain filling stage, leaves were collected from 5 plants per plot. Measurements of relative water content was done as follows: fresh weight (FW) was recorded immediately after leaf sampling; then leaves were soaked for 8 hours in distilled water at room temperature in the dark, and the turgid weight (TW) was recorded. Total dry weight (DW) was recorded after drying the leaves at 60° C. to a constant weight. Relative water content (RWC) is calculated according to Formula I (above).

Specific leaf area (SLA)—at grain filling stage, leaves were collected from 5 plants per plot. Leaves were scanned to obtain leaf area per plant, and then leaves were dried in an oven to obtain the leaves dry weight per plan. Specific leaf area was calculated by the leaf area divided by leaf dry weight.

Stomatal conductance (F) (GF) (mmol m⁻²s⁻¹)—plants were evaluated for their stomata conductance using SC-1 Leaf Porometer (Decagon devices) at flowering (F) and at grain filling (GF) stages. Stomata conductance readings were done on fully developed leaf, for 2 leaves and 2 plants per plot.

Upper internode length (cm), width (cm) and volume (cm³)—Upper internodes from at least 5 plants per plot were separated from the plant at flowering (F) and at harvest (H). Internodes were measured for their length (1) and width (d) using a ruler and a caliber. The internode volume was calculated using Formula VII.

internode volume=3.14×(d/2)²×1 Formula VII:

Upper internode fresh and dry density (F) and (H) (gr/cm³)—These parameters were measured at two time points during the course of the experiment: at flowering (F) and at harvest (H). Upper internodes from at least 5 plants per plot were separated from the plant and weighted (fresh and dry weight). To obtain stem density, stem weight (either fresh or dry) was divided by the stem volume (see above).

Lower internode length (cm), width (cm) and volume (cm³)—Lower internodes from at least 5 plants per plot were separated from the plant at flowering (F) and at harvest (H). Internodes were measured for their length (1) and width (d) using a ruler and a caliber. The internode volume was calculated using Formula VII above.

Lower internode fresh and dry density (F) and (H) (gr/cm³)—These parameters were measured at two time points during the course of the experiment: at flowering (F) and at harvest (H). Lower internodes from at least 5 plants per plot were separated from the plant and weighted (fresh and dry weight). To obtain stem density, stem weight (either fresh or dry) was divided by the stem volume (see above).

Number of days to heading [num]—Calculated as the number of days from sowing till 50% of the plot arrives heading.

Number of days to maturity [num]—Calculated as the number of days from sowing till 50% of the plot arrives seed maturation.

Maintenance of performance under drought conditions: Represent ratio for the specified parameter of Drought condition results divided by Normal conditions results (maintenance of phenotype under drought in comparison to normal conditions).

Data parameters collected are summarized in Tables 11-12, herein below.

TABLE 11

Sorghum correlated parameters under drought conditions (vectors)

Correlation

Correlated parameter with
ID

% Canopy coverage (GF) under drought
1

1000 seed weight under drought [gr]
2

Grain Circularity under drought [grain area/perimeter^2]
3

Grain Perimeter under drought [cm]
4

Grain area under drought [cm²]
5

Grain length under drought [cm]
6

Grain width under drought [cm]
7

Grain number under drought [num]
8

Total seed yield per plant under drought [gr]
9

Head DW (GF) under drought [gr]
10

Heads DW at harvest per plant under drought [gr]
11

Lower Stem dry density (H) under drought [gr/cm³]
12

Lower Stem fresh density (F) under drought [gr/cm³]
13

Lower Stem width (F) under drought [cm]
14

Main Head Area under drought [cm²]
15

Main Head Width under drought [cm]
16

Main Head length under drought [cm]
17

Number Days to heading (field) under drought [num]
18

Number days to maturity under drought [num]
19

Plant height under drought [cm]
20

RWC 2 under drought [%]
21

early GF under drought (SPAD unit)
22

SPAD late GF under drought (SPAD unit)
23

Specific leaf area (GF) under drought (cm²/gr)
24

Stomatal conductance (F) under drought [mmol m⁻²s⁻¹]
25

Stomatal conductance (GF) under drought [mmol m⁻²s⁻¹]
26

Upper internode dry density (H) under drought [gr/cm³]
27

Upper internode fresh density (H) under drought [gr/cm³]
28

Upper internode length (H) under drought [cm]
29

Upper internode volume (H) under drought [cm²]
30

Upper internode width (H) under drought [cm]
31

Vegetative DW per plant under drought [gr]
32

Vegetative FW per plant under drought [gr]
33

Table 11. Provided are the Sorghum correlated parameters (vectors). “gr.” = grams; “SPAD” = chlorophyll levels; “FW” = Plant Fresh weight; “DW” = Plant Dry weight; “GF” = grain filling growth stage; “F” = flowering stage; “H” = harvest stage.

TABLE 12

Sorghum correlated parameters for maintenance

under drought conditions (vectors)

Correlated parameter with
Correlation ID

1000 grains weight D/N
1

Grain Perimeter D/N
2

Grain area D/N
3

Grain length D/N
4

Grain width D/N
5

Grains num (SP) D/N
6

Grains weight per plant D/N
7

Head DW (GF) D/N
8

Heads weight per plant (RP) D/N
9

Lower Stem width (F) D/N
10

Lower stem dry density (H) D/N
11

Lower stem fresh density (F) D/N
12

Main Head Area D/N
13

Main Head Width D/N
14

Main Head length D/N
15

Plant height D/N
16

RWC_2 D/N
17

SPAD_2 D/N
18

SPAD_3 D/N
19

Specific leaf area (GF) D/N
20

Stomatal conductance (F) D/N
21

Stomatal conductance (GF) D/N
22

Upper Stem length (h) D/N
23

Upper Stem width (h) D/N
24

Upper stem dry density (H) D/N
25

Upper stem fresh density (H) D/N
26

Upper stem volume (H) D/N
27

Vegetative DW per plant D/N
28

Vegetative FW per plant D/N
29

Table 12. Provided are the Sorghum correlated parameters (vectors). “gr.” = grams; “SPAD” = chlorophyll levels; “FW” = Plant Fresh weight; “DW” = Plant Dry weight; “Maintenance under drought” = calculated % of change under drought vs. normal growth conditions. “GF” = grain filling growth stage; “F” = flowering stage; “H” = harvest stage. “D/N” = ratio for the specified parameter of Drought condition results divided by Normal conditions results (maintenance of phenotype under drought in comparison to normal conditions).

Experimental Results

Twelve different sorghum hybrids were grown and characterized for different parameters (Tables 11-12). The average for each of the measured parameter was calculated using the JMP software (Tables 13-18) and a subsequent correlation analysis was performed (Tables 19-20). Results were then integrated to the database.

TABLE 13

Measured parameters in Sorghum accessions under drought conditions

Line/

Corr. ID
1
2
3
4
5
6
7
8
9
10
11

Line-1
70.80
13.30
0.88
1.20
0.10
0.39
0.33
17494.21
29.22
19.29
0.04

Line-2
64.11
17.88
0.90
1.22
0.11
0.40
0.34
14526.20
31.74
33.15
0.04

Line-3
75.68
20.24
0.90
1.28
0.12
0.41
0.36
15728.96
40.21
27.31
0.05

Line-4
87.17
17.95
0.89
1.30
0.12
0.42
0.36
13808.50
29.52
50.38
0.03

Line-5
77.78
14.64
0.88
1.27
0.11
0.42
0.34
9838.55
18.23
37.04
0.02

Line-6
80.38
20.83
0.89
1.36
0.13
0.45
0.37
12402.52
34.43
11.72
0.02

Line-7
64.25
15.43
0.89
1.24
0.11
0.40
0.34
9979.86
19.10
9.32
0.03

Line-8
61.34
19.80
0.87
1.28
0.11
0.41
0.35
5451.71
13.67
12.10
0.02

Table 13: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Line) under drought conditions. Growth conditions are specified in the experimental procedure section.

TABLE 14

Additional measured parameters in Sorghum accessions under drought growth conditions

Line/

Corr. ID
12
13
14
15
16
17
18
19
20
21
22

Line-1
1.71
10.36
14.90
114.58
5.02
31.12
63.00
92.00
80.92
66.87
44.66

Line-2
1.66
11.28
13.32
94.24
5.57
22.16
56.00
92.00
93.43
68.62
51.92

Line-3
1.64
10.70
14.53
104.21
5.70
24.36
59.67
92.00
104.15
68.25
48.84

Line-4
1.60
9.68
17.27
87.37
4.77
24.76
76.67
107.00
105.63
76.33
37.60

Line-5
2.49
10.79
18.35
55.31
3.72
19.93
74.67
107.00
69.04
54.86
38.19

Line-6
1.25
9.66
13.96
85.87
5.81
19.41
71.00
107.00
133.54
74.51
43.35

Line-7
2.38
10.87
17.19
68.68
4.62
19.90
68.33
92.00
47.82
71.70
47.58

Line-8
1.60
10.46
16.63
96.62
5.53
24.79
66.33
92.00
83.24
78.51
46.97

Table 14: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under drought conditions. Growth conditions are specified in the experimental procedure section.

TABLE 15

Additional measured parameters in Sorghum accessions under drought growth conditions

Line/

Corr.

ID
23
24
25
26
27
28
29
30
31
32
33

Line-1
30.93
132.90
582.07
129.78
1.65
5.18
48.60
2865.28
8.60
0.03
0.09

Line-2
43.69
138.52
985.59
241.65
1.62
5.39
48.78
2857.93
8.59
0.03
0.10

Line-3
37.80
133.26
834.96
322.92
1.63
5.40
48.73
2955.99
8.73
0.04
0.11

Line-4
32.49
47.34
54.16
127.17
1.76
5.53
26.05
1288.48
7.85
0.08
0.19

Line-5
34.14
44.43
68.26
276.22
1.92
8.60
31.06
1128.61
6.63
0.06
0.15

Line-6
25.84
106.06
330.46
217.19
1.66
3.60
20.72
1724.93
10.20
0.05
0.11

Line-7
42.92
128.67
387.65
81.21
1.55
4.61
24.07
1507.76
8.88
0.04
0.08

Line-8
26.98
143.32
774.84
561.18
1.43
5.72
39.57
2524.28
8.92
0.04
0.10

Table 15: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under drought conditions. Growth conditions are specified in the experimental procedure section.

TABLE 16

Calculated parameters in Sorghum accessions under drought conditions (maintenance of

performance under drought vs normal growth conditions)

Line/

Corr. ID
1
2
3
4
5
6
7
8
9
10
11

Line-1
0.72
0.92
0.85
0.93
0.91
0.81
0.59
0.70
0.57
0.80
0.69

Line-2
0.76
0.93
0.87
0.94
0.93
1.11
0.81
1.05
0.64
0.98
0.65

Line-3
0.78
0.95
0.90
0.95
0.95
0.93
0.73
1.06
0.70
0.97
0.66

Line-4
0.88
0.99
0.97
0.99
0.97
0.56
0.46
0.68
0.42
1.05
0.97

Line-5
0.79
0.96
0.91
0.97
0.94
0.65
0.55
0.90
0.43
1.02
0.99

Line-6
0.77
0.94
0.88
0.93
0.94
0.71
0.57
0.77
0.36
0.87
0.70

Line-7
0.84
0.97
0.94
0.97
0.97
0.72
0.60
0.91
0.46
0.97
0.81

Line-8
0.87
0.98
0.96
0.98
0.98
0.87
0.76
0.94
0.52
0.99
0.79

Table 16: Provided are the values of each of the parameters (as described above) measured in sorghum accessions (line) for maintenance of performance under drought (calculated as % of change under drought vs normal growth conditions). Growth conditions are specified in the experimental procedure section

TABLE 17

Additional calculated parameters in Sorghum accessions under drought conditions

(maintenance of performance under drought vs normal growth conditions)

Line/

Corr. ID
12
13
14
15
16
17
18
19
20
21
22

Line-1
0.96
0.82
0.79
1.01
0.85
0.74
0.83
0.74
0.62
0.73
0.15

Line-2
1.04
0.95
0.94
0.98
0.92
0.76
0.99
0.93
0.65
1.12
0.33

Line-3
0.99
0.91
0.91
0.99
0.92
0.77
0.91
0.81
0.62
1.03
0.36

Line-4
0.92
0.59
0.75
0.81
0.64
0.93
0.85
0.81
0.70
0.09
0.33

Line-5
0.99
0.69
0.82
0.84
0.70
0.63
0.78
0.81
0.58
0.11
0.57

Line-6
0.96
0.77
0.81
0.94
0.79
0.82
0.92
0.77
0.65
0.60
0.34

Line-7
1.02
0.80
0.85
0.93
0.87
0.81
0.91
0.86
0.66
0.82
0.17

Line-8
0.98
1.20
1.11
1.15
0.80
0.86
0.95
0.76
0.84
0.76
0.69

Table 17: Provided are the values of each of the parameters (as described above) measured in sorghum accessions (line) for maintenance of performance under drought (calculated as % of change under drought vs normal growth conditions). Growth conditions are specified in the experimental procedure section.

TABLE 18

Additional calculated parameters in Sorghum accessions under drought conditions

(maintenance of performance under drought vs. normal growth conditions)

Line/Correlation ID
23
24
25
26
27
28
29

Line-1
0.81
1.00
0.95
0.71
0.82
0.74
0.60

Line-2
0.94
1.01
0.91
0.68
0.98
0.71
0.69

Line-3
0.89
0.95
0.98
0.77
0.81
0.69
0.59

Line-4
0.54
0.84
1.05
1.15
0.39
0.77
0.80

Line-5
0.59
0.86
1.05
1.04
0.46
0.82
0.68

Line-6
0.61
1.01
0.89
0.78
0.62
0.77
0.73

Line-7
0.84
1.05
0.88
0.64
0.94
0.85
0.66

Line-8
0.88
1.20
0.81
0.64
1.16
0.78
0.69

Table 18: Provided are the values of each of the parameters (as described above) measured in sorghum accessions (line) for maintenance of performance under drought (calculated as % of change under drought vs normal growth conditions). Growth conditions are specified in the experimental procedure section.

TABLE 19

Correlation between the expression level of selected LAB genes of some embodiments of the

invention in various tissues and the phenotypic performance under drought stress conditions

across Sorghum accessions

Gene

Exp.
Corr.
Gene

Exp.
Corr.

Name
R
P value
set
Set ID
Name
R
P value
set
Set ID

LAB740
0.73
6.52E−02
1
12
LAB740
0.78
3.97E−02
1
22

LAB740
0.87
1.09E−02
1
23
LAB740
0.89
7.81E−03
1
13

LAB741
0.86
1.39E−02
1
31
LAB741
0.72
6.94E−02
1
24

LAB741
0.78
3.98E−02
1
16
LAB741
0.94
1.38E−03
2
31

LAB744
0.86
1.29E−02
1
8
LAB744
0.90
5.88E−03
1
3

LAB744
0.74
5.54E−02
1
9
LAB744
0.83
1.96E−02
1
11

LAB744
0.81
2.78E−02
2
8
LAB744
0.75
5.17E−02
2
11

LAB745
0.87
1.15E−02
1
30
LAB745
0.94
1.84E−03
1
15

LAB745
0.77
4.35E−02
1
17
LAB745
0.88
8.68E−03
1
29

LAB745
0.75
5.16E−02
2
3
LAB746
0.83
1.98E−02
1
30

LAB746
0.79
3.57E−02
1
22
LAB746
0.83
1.96E−02
1
29

LAB746
0.92
3.14E−03
1
25
LAB746
0.75
5.17E−02
1
13

LAB746
0.75
5.31E−02
2
22
LAB746
0.83
2.07E−02
2
25

LAB746
0.73
6.03E−02
2
13
LAB748
0.77
4.10E−02
1
8

LAB748
0.90
6.41E−03
1
3
LAB748
0.85
1.50E−02
1
9

LAB748
0.97
4.04E−04
1
11
LAB749
0.85
1.65E−02
1
31

LAB749
0.72
6.77E−02
1
20
LAB749
0.73
6.39E−02
1
6

LAB749
0.71
7.42E−02
1
5
LAB749
0.71
7.51E−02
2
31

LAB749
0.77
4.29E−02
2
20
LAB749
0.71
7.41E−02
2
6

LAB750
0.93
2.69E−03
1
33
LAB750
0.92
2.85E−03
1
32

LAB750
0.81
2.77E−02
1
10
LAB750
0.72
7.01E−02
1
1

LAB750
0.71
7.54E−02
1
19
LAB750
0.84
1.83E−02
2
33

LAB750
0.87
1.07E−02
2
18
LAB750
0.89
6.59E−03
2
32

LAB750
0.94
1.53E−03
2
19
LAB750
0.83
2.08E−02
2
1

LAB751
0.72
6.58E−02
1
33
LAB751
0.84
1.87E−02
1
9

LAB751
0.82
2.52E−02
1
27
LAB751
0.77
4.29E−02
1
20

LAB751
0.95
1.16E−03
2
33
LAB751
0.82
2.50E−02
1
10

LAB751
0.98
1.19E−04
2
32
LAB751
0.82
2.32E−02
1
1

LAB751
0.82
2.48E−02
2
10
LAB751
0.72
6.64E−02
2
18

LAB751
0.90
6.33E−03
2
1
LAB751
0.80
3.03E−02
2
27

LAB752
0.80
2.90E−02
1
26
LAB751
0.81
2.87E−02
2
19

LAB752
0.84
1.87E−02
1
22
LAB752
0.84
1.81E−02
1
24

LAB752
0.80
3.25E−02
2
30
LAB752
0.88
8.13E−03
1
25

LAB753
0.94
1.84E−03
1
30
LAB752
0.83
1.95E−02
2
29

LAB753
0.70
7.72E−02
1
22
LAB753
0.72
6.70E−02
1
15

LAB753
0.82
2.40E−02
1
29
LAB753
0.77
4.27E−02
1
16

LAB753
0.72
6.59E−02
2
21
LAB753
0.90
5.75E−03
1
25

LAB754
0.93
2.11E−03
1
24
LAB753
0.91
4.67E−03
2
26

LAB754
0.78
3.75E−02
1
25
LAB754
0.93
2.34E−03
1
22

LAB754
0.75
5.04E−02
2
30
LAB754
0.89
7.98E−03
1
13

LAB754
0.98
1.80E−04
2
22
LAB754
0.90
5.55E−03
2
24

LAB754
0.89
6.68E−03
2
25
LAB754
0.81
2.75E−02
2
23

LAB755
0.71
7.47E−02
1
31
LAB754
0.89
7.62E−03
2
13

LAB755
0.92
3.81E−03
2
20
LAB755
0.72
6.66E−02
2
9

LAB756
0.87
1.18E−02
1
26
LAB755
0.70
7.88E−02
2
16

LAB756
0.94
1.77E−03
1
22
LAB756
0.98
1.40E−04
1
24

LAB756
0.78
3.68E−02
1
13
LAB756
0.83
1.97E−02
1
25

LAB757
0.73
6.15E−02
1
18
LAB756
0.77
4.46E−02
2
28

LAB757
0.75
5.45E−02
2
9
LAB757
0.88
8.49E−03
2
21

LAB758
0.78
3.90E−02
2
23
LAB758
0.71
7.26E−02
1
23

LAB759
0.79
3.50E−02
1
30
LAB758
0.72
6.55E−02
2
13

LAB759
0.93
2.15E−03
1
22
LAB759
0.91
4.75E−03
1
24

LAB759
0.89
7.44E−03
1
13
LAB759
0.92
2.95E−03
1
25

LAB759
0.72
6.71E−02
2
30
LAB759
0.82
2.42E−02
2
2

LAB759
0.92
3.75E−03
2
22
LAB759
0.75
5.02E−02
2
24

LAB759
0.93
2.41E−03
2
25
LAB759
0.81
2.60E−02
2
16

LAB760
0.87
1.18E−02
2
2
LAB759
0.88
9.71E−03
2
13

LAB760
0.74
5.60E−02
2
6
LAB760
0.73
6.19E−02
2
16

LAB760
0.80
3.12E−02
2
4
LAB760
0.78
3.97E−02
2
5

LAB761
0.87
9.98E−03
1
33
LAB760
0.81
2.58E−02
2
7

LAB761
0.77
4.30E−02
1
10
LAB761
0.91
4.18E−03
1
32

LAB761
0.78
3.95E−02
2
11
LAB761
0.80
3.24E−02
2
3

LAB762
0.80
2.99E−02
1
30
LAB761
0.77
4.34E−02
2
23

LAB762
0.88
8.21E−03
1
22
LAB762
0.76
4.66E−02
1
24

LAB762
0.95
9.59E−04
1
25
LAB762
0.76
4.65E−02
1
29

LAB762
0.73
6.14E−02
2
8
LAB762
0.91
4.95E−03
1
13

LAB762
0.88
8.68E−03
2
12
LAB762
0.79
3.32E−02
2
14

LAB763
0.80
3.16E−02
1
24
LAB762
0.90
5.40E−03
2
11

LAB763
0.76
4.67E−02
1
25
LAB763
0.91
4.92E−03
1
22

LAB763
0.97
3.53E−04
2
12
LAB763
0.91
4.86E−03
1
13

LAB764
0.74
5.89E−02
1
26
LAB763
0.71
7.23E−02
2
23

LAB764
0.74
5.79E−02
2
33
LAB764
0.92
3.27E−03
2
21

LAB764
0.83
1.94E−02
2
18
LAB764
0.80
3.14E−02
2
32

LAB764
0.77
4.38E−02
2
5
LAB764
0.78
3.90E−02
2
6

LAB764
0.81
2.57E−02
2
19
LAB764
0.81
2.71E−02
2
4

LAB765
0.72
6.87E−02
1
30
LAB764
0.71
7.12E−02
2
7

LAB765
0.73
6.32E−02
1
23
LAB765
0.83
2.13E−02
1
22

LAB765
0.84
1.76E−02
1
25
LAB765
0.80
3.23E−02
1
29

LAB765
0.78
4.04E−02
2
10
LAB765
0.93
2.21E−03
1
13

LAB767
0.86
1.38E−02
1
25
LAB767
0.82
2.53E−02
1
22

LAB767
0.70
7.75E−02
2
23
LAB767
0.71
7.62E−02
2
8

LAB768
0.95
1.04E−03
1
30
LAB767
0.82
2.26E−02
2
13

LAB768
0.76
4.77E−02
1
15
LAB768
0.71
7.43E−02
1
11

LAB768
0.90
6.02E−03
1
29
LAB768
0.73
6.28E−02
1
16

LAB768
0.71
7.48E−02
2
21
LAB768
0.84
1.86E−02
1
25

LAB768
0.90
5.54E−03
2
32
LAB768
0.89
7.62E−03
2
33

LAB768
0.78
3.84E−02
2
10
LAB768
0.73
6.16E−02
2
18

LAB769
0.81
2.65E−02
1
9
LAB769
0.78
3.94E−02
1
2

LAB769
0.73
6.46E−02
1
6
LAB769
0.72
6.90E−02
1
5

LAB769
0.75
5.20E−02
1
4
LAB769
0.70
7.76E−02
1
1

LAB769
0.72
6.96E−02
1
7
LAB769
0.70
7.76E−02
2
21

LAB769
0.93
2.14E−03
2
33
LAB769
0.79
3.58E−02
2
18

LAB769
0.97
3.62E−04
2
32
LAB769
0.87
1.10E−02
2
27

LAB769
0.76
4.90E−02
2
10
LAB769
0.78
3.83E−02
2
19

LAB769
0.89
6.94E−03
2
1
LAB770
0.76
4.86E−02
1
9

LAB770
0.91
5.01E−03
1
3
LAB770
0.72
7.02E−02
1
10

LAB770
0.72
6.79E−02
1
11
LAB770
0.94
1.35E−03
2
8

LAB770
0.83
2.06E−02
1
27
LAB770
0.82
2.43E−02
2
18

LAB770
0.77
4.27E−02
2
32
LAB770
0.79
3.48E−02
2
19

LAB770
0.80
2.94E−02
2
27
LAB771
0.72
6.57E−02
1
22

LAB771
0.92
3.42E−03
1
24
LAB771
0.72
6.59E−02
2
23

LAB771
0.90
5.99E−03
2
11
LAB772
0.72
6.96E−02
1
33

LAB771
0.72
6.70E−02
2
13
LAB774
0.74
5.48E−02
1
17

LAB772
0.77
4.45E−02
1
10
LAB775
0.83
2.19E−02
1
33

LAB774
0.82
2.52E−02
2
26
LAB775
0.71
7.54E−02
1
20

LAB775
0.73
6.50E−02
1
15
LAB775
0.79
3.30E−02
1
10

LAB775
0.72
7.01E−02
1
27
LAB775
0.80
3.17E−02
1
1

LAB775
0.83
2.19E−02
2
8
LAB775
0.80
3.01E−02
2
9

LAB775
0.70
7.97E−02
2
10
LAB775
0.82
2.29E−02
2
27

LAB775
0.74
5.59E−02
2
1
LAB776
0.85
1.45E−02
1
30

LAB776
0.82
2.54E−02
1
26
LAB776
0.74
5.89E−02
1
24

LAB776
0.78
3.96E−02
1
29
LAB776
0.89
7.85E−03
1
25

LAB776
0.87
1.12E−02
2
30
LAB776
0.80
3.23E−02
2
15

LAB776
0.77
4.38E−02
2
24
LAB776
0.82
2.43E−02
2
29

LAB776
0.73
6.23E−02
2
25
LAB777
0.71
7.23E−02
1
33

LAB777
0.78
3.83E−02
1
10
LAB777
0.76
4.91E−02
1
27

LAB777
0.71
7.66E−02
2
2
LAB777
0.91
3.97E−03
2
6

LAB777
0.87
1.13E−02
2
5
LAB777
0.88
9.54E−03
2
4

LAB777
0.78
4.00E−02
2
7
LAB778
0.92
3.02E−03
1
33

LAB778
0.92
3.36E−03
1
32
LAB778
0.93
2.77E−03
1
10

LAB778
0.76
4.62E−02
1
27
LAB778
0.75
5.43E−02
1
1

LAB778
0.83
1.94E−02
2
8
LAB778
0.77
4.27E−02
2
3

LAB778
0.75
5.05E−02
2
12
LAB778
0.79
3.29E−02
2
11

LAB779
0.85
1.52E−02
1
28
LAB779
0.81
2.87E−02
1
10

LAB779
0.75
5.10E−02
2
33
LAB779
0.71
7.55E−02
2
32

LAB779
0.77
4.51E−02
2
10
LAB780
0.88
9.27E−03
1
31

LAB780
0.88
8.94E−03
2
21
LAB780
0.87
1.18E−02
2
33

LAB780
0.79
3.63E−02
2
32
LAB780
0.77
4.22E−02
2
6

LAB780
0.71
7.31E−02
2
5
LAB780
0.78
3.82E−02
2
4

LAB781
0.89
7.09E−03
1
33
LAB781
0.70
7.74E−02
1
20

LAB781
0.78
3.88E−02
1
10
LAB781
0.80
3.11E−02
1
32

LAB781
0.78
3.71E−02
1
1
LAB781
0.75
5.06E−02
1
27

LAB781
0.91
4.22E−03
2
31
LAB781
0.81
2.67E−02
1
19

LAB781
0.89
6.90E−03
2
6
LAB781
0.70
7.96E−02
2
20

LAB781
0.87
1.15E−02
2
4
LAB781
0.86
1.21E−02
2
5

LAB783
0.77
4.51E−02
2
11
LAB781
0.77
4.10E−02
2
7

LAB784
0.86
1.34E−02
1
24
LAB784
0.83
2.19E−02
1
30

LAB784
0.71
7.51E−02
1
29
LAB784
0.76
4.81E−02
1
22

LAB784
0.93
2.83E−03
2
21
LAB784
0.80
2.91E−02
1
25

LAB784
0.85
1.53E−02
2
32
LAB784
0.79
3.55E−02
2
33

LAB784
0.72
6.98E−02
2
4
LAB784
0.84
1.92E−02
2
18

LAB785
0.72
6.78E−02
1
31
LAB784
0.78
4.03E−02
2
19

LAB785
0.70
7.85E−02
2
6
LAB785
0.78
3.89E−02
2
31

LAB786
0.80
2.90E−02
2
17
LAB786
0.70
7.72E−02
1
14

LAB787
0.79
3.55E−02
1
24
LAB787
0.73
6.10E−02
1
12

LAB787
0.84
1.68E−02
1
13
LAB787
0.85
1.67E−02
1
22

LAB789
0.80
2.90E−02
1
31
LAB788
0.89
6.60E−03
2
26

LAB789
0.84
1.81E−02
2
6
LAB789
0.88
8.12E−03
2
2

LAB789
0.88
9.25E−03
2
4
LAB789
0.87
1.14E−02
2
5

LAB790
0.88
8.23E−03
1
30
LAB789
0.88
8.10E−03
2
7

LAB790
0.82
2.40E−02
1
24
LAB790
0.82
2.47E−02
1
26

LAB790
0.70
7.97E−02
1
16
LAB790
0.80
3.12E−02
1
22

LAB790
0.95
1.02E−03
1
25
LAB790
0.76
4.54E−02
1
29

LAB790
0.93
2.44E−03
2
26
LAB790
0.75
5.03E−02
2
2

LAB792
0.76
4.66E−02
1
22
LAB791
0.81
2.71E−02
1
17

LAB792
0.74
5.47E−02
1
13
LAB792
0.78
3.85E−02
1
25

LAB792
0.80
2.90E−02
2
33
LAB792
0.72
6.68E−02
2
21

LAB792
0.71
7.31E−02
2
18
LAB792
0.85
1.57E−02
2
32

LAB793
0.89
7.98E−03
1
10
LAB793
0.76
4.54E−02
1
33

LAB793
0.77
4.10E−02
2
24
LAB793
0.75
5.08E−02
2
11

LAB793
0.84
1.84E−02
2
23
LAB793
0.91
4.74E−03
2
22

LAB793
0.82
2.31E−02
2
25
LAB793
0.71
7.66E−02
2
29

LAB794
0.94
1.67E−03
1
30
LAB793
0.98
8.22E−05
2
13

LAB794
0.70
7.99E−02
1
24
LAB794
0.93
2.15E−03
1
15

LAB794
0.96
7.10E−04
1
29
LAB794
0.70
7.98E−02
1
17

LAB794
0.71
7.23E−02
2
15
LAB794
0.80
3.05E−02
1
25

LAB795
0.74
5.92E−02
2
31
LAB794
0.79
3.35E−02
2
17

LAB796
0.86
1.24E−02
2
11
LAB796
0.89
7.76E−03
2
3

LAB797
0.91
4.11E−03
1
12
LAB796
0.73
6.48E−02
2
23

LAB798
0.73
6.24E−02
1
9
LAB797
0.77
4.21E−02
2
12

LAB799
0.79
3.28E−02
2
12
LAB798
0.70
7.76E−02
1
20

LAB799
0.92
3.04E−03
2
23
LAB799
0.80
3.01E−02
1
12

LAB799
0.91
4.50E−03
2
13

Table 19. Correlations (R) between the genes expression levels in various tissues and the phenotypic performance. “Con. ID” - correlation set ID according to the correlated parameters Table above. “Exp. Set” - Expression set. “R” = Pearson correlation coefficient; “P” = p value.

TABLE 20

Correlation between the expression level of selected LAB genes of some embodiments of the

invention in various tissues and the phenotypic performance of maintenance of performance

under drought across Sorghum accessions

Gene

Exp.
Corr.
Gene

Exp.
Corr.

Name
R
P value
set
Set ID
Name
R
P value
set
Set ID

LAB740
0.74
5.77E−02
1
8
LAB740
0.78
3.83E−02
1
23

LAB740
0.77
4.21E−02
1
21
LAB740
0.80
3.26E−02
1
12

LAB740
0.85
1.49E−02
1
16
LAB740
0.72
6.94E−02
1
9

LAB741
0.78
3.92E−02
1
15
LAB741
0.78
3.69E−02
1
13

LAB741
0.89
7.19E−03
1
24
LAB741
0.74
5.60E−02
1
27

LAB741
0.75
5.24E−02
1
14
LAB742
0.75
5.13E−02
2
19

LAB742
0.76
4.96E−02
2
6
LAB744
0.76
4.73E−02
1
19

LAB745
0.73
6.06E−02
2
19
LAB746
0.78
3.77E−02
1
8

LAB746
0.78
3.92E−02
1
23
LAB746
0.75
5.30E−02
1
21

LAB746
0.74
5.94E−02
1
13
LAB746
0.95
1.09E−03
1
6

LAB746
0.70
7.94E−02
1
14
LAB746
0.80
2.97E−02
1
9

LAB746
0.90
5.69E−03
1
7
LAB746
0.74
5.87E−02
2
8

LAB746
0.71
7.62E−02
2
12
LAB746
0.91
4.86E−03
2
6

LAB746
0.74
5.73E−02
2
18
LAB746
0.85
1.49E−02
2
7

LAB748
0.76
4.80E−02
1
19
LAB748
0.73
6.08E−02
1
6

LAB750
0.70
7.99E−02
1
25
LAB750
0.77
4.12E−02
1
29

LAB750
0.95
1.04E−03
1
26
LAB750
0.82
2.47E−02
1
11

LAB750
0.78
3.67E−02
1
17
LAB750
0.82
2.42E−02
2
29

LAB750
0.86
1.23E−02
2
26
LAB750
0.80
3.06E−02
2
17

LAB751
0.81
2.81E−02
1
25
LAB751
0.73
6.18E−02
1
26

LAB751
0.89
7.98E−03
2
25
LAB751
0.98
1.71E−04
2
26

LAB751
0.70
7.76E−02
2
11
LAB751
0.75
5.15E−02
2
17

LAB752
0.73
6.04E−02
1
8
LAB752
0.82
2.46E−02
1
23

LAB752
0.79
3.64E−02
1
21
LAB752
0.76
4.91E−02
1
12

LAB752
0.96
5.72E−04
1
15
LAB752
0.93
2.36E−03
1
13

LAB752
0.87
9.97E−03
1
24
LAB752
0.85
1.49E−02
1
6

LAB752
0.90
5.32E−03
1
27
LAB752
0.88
8.53E−03
1
14

LAB752
0.81
2.72E−02
1
18
LAB752
0.94
1.60E−03
1
7

LAB753
0.74
5.84E−02
1
21
LAB753
0.71
7.40E−02
1
13

LAB753
0.91
4.77E−03
1
6
LAB753
0.75
5.32E−02
1
9

LAB753
0.86
1.42E−02
1
7
LAB753
0.95
1.00E−03
2
22

LAB753
0.89
7.99E−03
2
20
LAB753
0.74
5.61E−02
2
14

LAB754
0.82
2.27E−02
1
8
LAB754
0.93
2.14E−03
1
23

LAB754
0.94
1.94E−03
1
21
LAB754
0.87
1.04E−02
1
12

LAB754
0.75
5.00E−02
1
6
LAB754
0.83
1.96E−02
1
27

LAB754
0.94
1.62E−03
1
16
LAB754
0.75
5.42E−02
1
7

LAB754
0.84
1.89E−02
2
8
LAB754
0.94
1.67E−03
2
23

LAB754
0.99
5.34E−06
2
21
LAB754
0.87
1.06E−02
2
12

LAB754
0.88
8.53E−03
2
6
LAB754
0.76
4.72E−02
2
27

LAB754
0.98
1.17E−04
2
16
LAB754
0.78
3.66E−02
2
9

LAB754
0.85
1.48E−02
2
7
LAB756
0.79
3.55E−02
1
8

LAB756
0.90
5.40E−03
1
23
LAB756
0.95
9.87E−04
1
21

LAB756
0.74
5.97E−02
1
22
LAB756
0.85
1.62E−02
1
12

LAB756
0.74
5.61E−02
1
15
LAB756
0.85
1.47E−02
1
13

LAB756
0.79
3.55E−02
1
6
LAB756
0.87
1.08E−02
1
27

LAB756
0.91
4.01E−03
1
16
LAB756
0.89
6.67E−03
1
14

LAB756
0.83
2.20E−02
1
7
LAB756
0.74
5.56E−02
2
22

LAB756
0.76
4.85E−02
2
20
LAB757
0.74
5.84E−02
2
29

LAB757
0.76
4.81E−02
2
20
LAB758
0.95
9.02E−04
1
19

LAB758
0.79
3.56E−02
1
10
LAB758
0.88
8.09E−03
2
19

LAB759
0.77
4.21E−02
1
8
LAB759
0.97
3.94E−04
1
23

LAB759
0.89
6.53E−03
1
21
LAB759
0.85
1.46E−02
1
12

LAB759
0.76
4.96E−02
1
15
LAB759
0.82
2.28E−02
1
13

LAB759
0.90
5.81E−03
1
6
LAB759
0.92
2.94E−03
1
27

LAB759
0.90
5.80E−03
1
16
LAB759
0.75
5.39E−02
1
14

LAB759
0.70
7.74E−02
1
9
LAB759
0.92
3.44E−03
1
7

LAB759
0.82
2.46E−02
2
8
LAB759
0.88
9.15E−03
2
23

LAB759
0.86
1.28E−02
2
21
LAB759
0.86
1.27E−02
2
12

LAB759
0.72
7.09E−02
2
13
LAB759
0.96
4.89E−04
2
6

LAB759
0.77
4.13E−02
2
27
LAB759
0.75
5.04E−02
2
16

LAB759
0.76
4.53E−02
2
18
LAB759
0.73
6.26E−02
2
9

LAB759
0.94
1.50E−03
2
7
LAB761
0.75
5.15E−02
1
25

LAB761
0.92
3.47E−03
1
26
LAB761
0.71
7.41E−02
1
4

LAB761
0.86
1.20E−02
1
11
LAB761
0.76
4.82E−02
1
17

LAB762
0.92
3.15E−03
1
8
LAB762
0.91
4.25E−03
1
23

LAB762
0.87
1.02E−02
1
21
LAB762
0.77
4.12E−02
1
12

LAB762
0.76
4.73E−02
1
13
LAB762
0.91
4.79E−03
1
6

LAB762
0.75
5.19E−02
1
27
LAB762
0.79
3.63E−02
1
16

LAB762
0.74
5.64E−02
1
14
LAB762
0.88
9.59E−03
1
9

LAB762
0.95
1.02E−03
1
7
LAB762
0.70
7.88E−02
2
25

LAB763
0.92
3.57E−03
1
8
LAB763
0.87
1.01E−02
1
23

LAB763
0.82
2.42E−02
1
21
LAB763
0.95
1.18E−03
1
12

LAB763
0.71
7.28E−02
1
13
LAB763
0.77
4.20E−02
1
6

LAB763
0.89
7.20E−03
1
27
LAB763
0.73
6.31E−02
1
16

LAB763
0.77
4.32E−02
1
14
LAB763
0.88
8.10E−03
1
18

LAB763
0.86
1.30E−02
1
7
LAB763
0.70
7.88E−02
2
28

LAB764
0.80
3.00E−02
1
15
LAB764
0.89
7.69E−03
1
13

LAB764
0.80
2.92E−02
1
24
LAB764
0.87
1.12E−02
1
27

LAB764
0.74
5.47E−02
1
20
LAB764
0.91
4.53E−03
1
14

LAB764
0.75
5.36E−02
1
18
LAB764
0.76
4.61E−02
1
7

LAB764
0.70
7.88E−02
2
1
LAB764
0.83
2.16E−02
2
29

LAB764
0.92
3.42E−03
2
17
LAB765
0.76
4.58E−02
1
8

LAB765
0.87
1.08E−02
1
23
LAB765
0.79
3.42E−02
1
21

LAB765
0.76
4.66E−02
1
12
LAB765
0.88
9.55E−03
1
6

LAB765
0.76
4.95E−02
1
16
LAB765
0.88
8.64E−03
1
9

LAB765
0.79
3.36E−02
1
7
LAB765
0.71
7.36E−02
2
19

LAB765
0.79
3.50E−02
2
10
LAB767
0.93
2.20E−03
1
8

LAB767
0.70
7.78E−02
1
23
LAB767
0.79
3.44E−02
1
21

LAB767
0.72
6.96E−02
1
12
LAB767
0.88
9.55E−03
1
6

LAB767
0.78
3.83E−02
1
18
LAB767
0.81
2.66E−02
1
9

LAB767
0.90
5.14E−03
1
7
LAB768
0.74
5.83E−02
1
21

LAB768
0.86
1.38E−02
1
6
LAB768
0.73
6.32E−02
1
16

LAB768
0.82
2.52E−02
1
9
LAB768
0.74
5.50E−02
2
1

LAB768
0.82
2.49E−02
2
29
LAB768
0.85
1.52E−02
2
26

LAB768
0.77
4.36E−02
2
4
LAB768
0.75
5.00E−02
2
2

LAB768
0.71
7.44E−02
2
3
LAB768
0.87
1.15E−02
2
11

LAB768
0.93
2.37E−03
2
17
LAB769
0.86
1.22E−02
2
25

LAB769
0.79
3.55E−02
2
29
LAB769
0.95
1.12E−03
2
26

LAB769
0.73
6.15E−02
2
2
LAB769
0.79
3.39E−02
2
11

LAB769
0.93
2.41E−03
2
17
LAB770
0.90
6.26E−03
1
25

LAB770
0.70
7.81E−02
1
26
LAB770
0.81
2.60E−02
2
25

LAB770
0.71
7.22E−02
2
26
LAB771
0.78
3.75E−02
1
23

LAB771
0.80
3.10E−02
1
21
LAB771
0.72
6.93E−02
1
27

LAB771
0.85
1.55E−02
1
16
LAB771
0.85
1.46E−02
2
9

LAB772
0.77
4.10E−02
1
26
LAB772
0.78
3.92E−02
1
11

LAB774
0.76
4.72E−02
2
22
LAB775
0.80
3.17E−02
1
25

LAB775
0.81
2.80E−02
1
26
LAB775
0.79
3.43E−02
2
25

LAB776
0.75
5.38E−02
1
23
LAB776
0.88
8.98E−03
1
15

LAB776
0.90
5.90E−03
1
13
LAB776
0.82
2.52E−02
1
6

LAB776
0.77
4.42E−02
1
27
LAB776
0.82
2.43E−02
1
14

LAB776
0.86
1.21E−02
1
7
LAB776
0.76
4.92E−02
2
23

LAB776
0.72
6.63E−02
2
15
LAB776
0.71
7.24E−02
2
27

LAB777
0.73
6.32E−02
1
25
LAB777
0.78
3.93E−02
1
26

LAB778
0.79
3.42E−02
1
25
LAB778
0.88
8.16E−03
1
26

LAB778
0.73
6.44E−02
1
17
LAB779
0.78
3.92E−02
2
25

LAB779
0.75
5.11E−02
2
26
LAB780
0.82
2.51E−02
1
15

LAB780
0.82
2.27E−02
1
24
LAB780
0.73
6.52E−02
2
22

LAB780
0.78
4.05E−02
2
26
LAB780
0.81
2.64E−02
2
20

LAB780
0.70
7.95E−02
2
17
LAB781
0.78
3.85E−02
1
29

LAB781
0.85
1.48E−02
1
26
LAB783
0.71
7.23E−02
1
1

LAB783
0.75
5.09E−02
1
22
LAB783
0.72
6.75E−02
1
4

LAB783
0.79
3.40E−02
1
20
LAB783
0.71
7.22E−02
1
14

LAB783
0.84
1.75E−02
1
10
LAB783
0.73
6.29E−02
2
25

LAB784
0.72
6.68E−02
1
23
LAB784
0.82
2.31E−02
1
21

LAB784
0.93
2.83E−03
1
15
LAB784
0.82
2.29E−02
1
13

LAB784
0.71
7.64E−02
1
24
LAB784
0.81
2.73E−02
1
6

LAB784
0.78
3.86E−02
1
27
LAB784
0.81
2.85E−02
1
16

LAB784
0.70
7.83E−02
1
7
LAB784
0.80
3.25E−02
2
1

LAB784
0.87
1.08E−02
2
29
LAB784
0.72
7.00E−02
2
26

LAB784
0.73
6.15E−02
2
4
LAB784
0.77
4.28E−02
2
2

LAB784
0.73
6.41E−02
2
20
LAB784
0.76
4.94E−02
2
3

LAB784
0.79
3.33E−02
2
11
LAB784
0.97
3.93E−04
2
17

LAB784
0.71
7.57E−02
2
5
LAB787
0.72
7.03E−02
1
8

LAB787
0.80
2.98E−02
1
23
LAB787
0.81
2.79E−02
1
21

LAB787
0.89
6.49E−03
1
12
LAB787
0.77
4.25E−02
1
27

LAB787
0.81
2.62E−02
1
16
LAB788
0.81
2.68E−02
2
22

LAB788
0.72
6.92E−02
2
15
LAB789
0.71
7.55E−02
1
24

LAB790
0.78
3.98E−02
1
8
LAB790
0.83
2.23E−02
1
23

LAB790
0.83
2.16E−02
1
21
LAB790
0.85
1.62E−02
1
15

LAB790
0.95
8.48E−04
1
13
LAB790
0.71
7.20E−02
1
24

LAB790
0.87
1.02E−02
1
6
LAB790
0.86
1.25E−02
1
27

LAB790
0.73
6.48E−02
1
16
LAB790
0.95
1.07E−03
1
14

LAB790
0.78
3.82E−02
1
18
LAB790
0.78
3.70E−02
1
9

LAB790
0.95
8.94E−04
1
7
LAB790
0.91
4.69E−03
2
22

LAB790
0.72
7.03E−02
2
15
LAB790
0.72
6.94E−02
2
13

LAB790
0.71
7.39E−02
2
20
LAB790
0.74
5.97E−02
2
14

LAB792
0.92
3.53E−03
1
8
LAB792
0.72
6.88E−02
1
23

LAB792
0.73
6.08E−02
1
12
LAB792
0.71
7.29E−02
1
13

LAB792
0.78
3.76E−02
1
6
LAB792
0.81
2.58E−02
1
14

LAB792
0.88
9.19E−03
1
18
LAB792
0.89
8.00E−03
1
7

LAB792
0.73
6.15E−02
2
1
LAB792
0.75
5.38E−02
2
26

LAB792
0.73
6.04E−02
2
4
LAB792
0.78
4.06E−02
2
2

LAB792
0.73
6.03E−02
2
3
LAB792
0.77
4.39E−02
2
11

LAB792
0.88
8.35E−03
2
17
LAB793
0.71
7.57E−02
1
25

LAB793
0.71
7.59E−02
1
26
LAB793
0.79
3.63E−02
2
8

LAB793
0.95
1.12E−03
2
23
LAB793
0.89
7.66E−03
2
21

LAB793
0.85
1.53E−02
2
12
LAB793
0.83
2.19E−02
2
6

LAB793
0.73
6.37E−02
2
27
LAB793
0.89
7.10E−03
2
16

LAB793
0.83
2.20E−02
2
9
LAB793
0.77
4.19E−02
2
7

LAB794
0.73
6.24E−02
1
23
LAB794
0.73
6.03E−02
1
15

LAB794
0.75
5.16E−02
1
6
LAB794
0.82
2.31E−02
1
9

LAB796
0.86
1.37E−02
1
8
LAB796
0.78
4.05E−02
1
12

LAB796
0.70
7.83E−02
1
24
LAB796
0.71
7.24E−02
1
27

LAB796
0.76
4.79E−02
1
18
LAB796
0.72
6.82E−02
2
16

LAB799
0.74
5.81E−02
1
28
LAB799
0.77
4.22E−02
1
24

LAB799
0.76
4.58E−02
2
28
LAB799
0.72
6.63E−02
2
8

LAB799
0.78
3.91E−02
2
23
LAB799
0.82
2.44E−02
2
19

LAB799
0.72
6.64E−02
2
12
LAB799
0.75
5.23E−02
2
9

Table 20. Correlations (R) between the genes expression levels in vanous tissues and the phenotypic performance. “Con. ID” - correlation set ID according to the correlated parameters Table above. “Exp. Set” - Expression set. “R” = Pearson correlation coefficient; “P” = p value.

Example 4
Production of Sorghum Transcriptom and High Throughput Correlation Analysis with Biomass, Nue, and Abst Related Parameters Measured in Semi-Hydroponics Conditions Using 44K Sorguhm Oligonucleotide Micro-Arrays

Sorghum vigor related parameters under high salinity (100 mM NaCl), low temperature (10±2° C.), low nitrogen conditions and normal growth conditions—Ten Sorghum hybrids were grown in 3 repetitive plots, each containing 17 plants, at a net house under semi-hydroponics conditions. Briefly, the growing protocol was as follows: Sorghum seeds were sown in trays filled with a mix of vermiculite and peat in a 1:1 ratio. Following germination, the trays were transferred to Normal growth conditions (Full Hoagland containing 16 mM Nitrogen solution, at 28±2° C.), high salinity conditions (100 mM NaCl in addition to the Full Hoagland solution), low temperature conditions (10±2° C. in the presence of Full Hoagland solution), or low nitrogen conditions (the amount of total nitrogen was reduced in 90% from the full Hoagland solution (i.e., to a final concentration of 10% from full Hoagland solution, final amount of 1.2 mM Nitrogen). All plants were grown at 28±2° C. except where otherwise indicated (i.e., in the low temperature conditions).

Full Hoagland solution consists of: KNO₃—0.808 grams/liter, MgSO₄—0.12 grams/liter, KH₂PO₄—0.172 grams/liter and 0.01% (volume/volume) of ‘Super coratin’ micro elements (Iron-EDDHA [ethylenediamine-N,N′-bis(2-hydroxyphenylacetic acid)]—40.5 grams/liter; Mn—20.2 grams/liter; Zn 10.1 grams/liter; Co 1.5 grams/liter; and Mo 1.1 grams/liter), solution's pH should be 6.5-6.8].

Analyzed Sorghum tissues—All 10 selected Sorghum hybrids were sampled per each treatment. Three tissues [leaves, meristems and roots] growing at 100 mM NaCl, low temperature (10±2° C.), low Nitrogen (1.2 mM Nitrogen) or under Normal conditions were sampled and RNA was extracted as described above. Each micro-array expression information tissue type has received a Set ID as summarized in Table 21 below.

TABLE 21

Sorghum transcriptom expression sets under

semi hydroponics conditions

Expression Set
Set ID

Sorghum bath/Cold/root
1

Sorghum bath/Normal/root
2

Sorghum bath/NUE/root
3

Sorghum bath/NaCl/root
4

Sorghum bath/Cold/vegetative meristem
5

Sorghum bath/NUE/vegetative meristem
6

Sorghum bath/NaCl/vegetative meristem
7

Sorghum bath/Normal/vegetative meristem
8

Table 21: Provided are the Sorghum transcriptom expression sets. Cold conditions = 10 ± 2° C.; NaCl = 100 mM NaCl; low nitrogen = 1.2 mM Nitrogen; Normal conditions = 16 mM Nitrogen.

Sorghum Biomass, Vigor, Nitrogen Use Efficiency and Growth-Related Components

Root DW [gr]—At the end of the experiment, the root material was collected, measured and divided by the number of Plants.

Shoot DW [gr]—At the end of the experiment, the shoot material (without roots) was collected, measured and divided by the number of Plants.

Total biomass [gr]—total biomass including roots and shoots.

Leaf num [num]—number op opened leaves.

RGR Leaf Num—calculated relative growth rate (RGR) of leaf number.

Shoot/Root—biomass of shoot divided by biomass of roots.

NUE per total biomass—nitrogen use efficiency (NUE) of total biomass.

NUE per root biomass—nitrogen use efficiency (NUE) of total biomass.

NUE per shoot biomass—nitrogen use efficiency (NUE) of total biomass.

Percent of reduction of root biomass compared to normal—the difference (reduction in percent) between root biomass under normal and under low nitrogen conditions.

Percent of reduction of shoot biomass compared to normal—the difference (reduction in percent) between shoot biomass under normal and under low nitrogen conditions.

Percent of reduction of total biomass compared to normal—the difference (reduction in percent) between total biomass (shoot and root) under normal and under low nitrogen conditions.

Plant height [cm]—Plants were characterized for height during growing period at 5 time points. In each measure, plants were measured for their height using a measuring tape. Height was measured from ground level to top of the longest leaf.

SPAD [SPAD unit]—Chlorophyll content was determined using a Minolta SPAD 502 chlorophyll meter and measurement was performed 64 days post sowing. SPAD meter readings were done on young fully developed leaf. Three measurements per leaf were taken per plot.

Root Biomass [DW, gr.]/SPAD—root biomass divided by SPAD results.

Shoot Biomass [DW, gr.]/SPAD—shoot biomass divided by SPAD results.

Total Biomass (Root+Shoot) [DW, gr.]/SPAD—total biomass divided by SPAD results.

Experimental Results

10 different Sorghum hybrids were grown and characterized for various biomass and nitrogen use efficiency (NUE) parameters as described in Table 22, below. The average for each of the measured parameter was calculated using the JMP software and values are summarized in Table 23-30 below. Subsequent correlation analysis was performed (Table 31). Results were then integrated to the database.

TABLE 22

Sorghum correlated parameters (vectors)

Correlated parameter with
Correlation ID

Root DW/Plant at 100 mM NaCl [gr]
1

Root DW/Plant at Cold [gr]
2

Root DW/Plant at Low Nitrogen [gr]
3

Root DW/Plant at Normal [gr]
4

Shoot DW/Plant at Low Nitrogen [gr]
5

Shoot DW/Plant at 100 mM NaCl [gr]
6

Shoot DW/Plant at Cold [gr]
7

Shoot DW/Plant at Normal [gr]
8

Leaf num TP1 at 100 mM NaCl [num]
9

Leaf num TP1 at Cold [num]
10

Leaf num TP1 - Low Nitrogen [num]
11

Leaf num TP1 - Normal [num]
12

Leaf num TP2 - 100 mM NaCl [num]
13

Leaf num TP2 - Cold [num]
14

Leaf num TP2 - Low Nitrogen [num]
15

Leaf num TP2 - Normal [num]
16

Leaf num TP3 - 100 mM NaCl [num]
17

Leaf num TP3 - Cold [num]
18

Leaf num TP3 - Low Nitrogen [num]
19

Leaf num TP3 - Normal [num]
20

NUE per total biomass - Low N-
21

Shoot/Root - Low N-
22

NUE per root biomass Low N-NUE roots
23

NUE per shoot biomass Low N-NUE shoots
24

Percent of reduction of root biomass compared to
25

normal - Low N-

Percent of reduction of shoot biomass compared to
26

normal Low N-

Percent of reduction of total biomass compared to normal
27

Low N-percent-total biomass reduction compared to

normal

N level/Leaf [Low Nitrogen]
28

N level/Leaf [100 mM NaCl]
29

N level/Leaf [Cold]
30

N level/Leaf [Normal]
31

Shoot/Root - Normal - Shoot/Root
32

NUE per root biomass - Normal-
33

NUE per shoot biomass - Normal-
34

NUE per total biomass - Normal-
35

Plant Height TP1 - 100 mM NaCl [cm]
36

Plant Height TP1 - Cold [cm]
37

Plant Height TP1 - Low Nitrogen [cm]
38

Plant Height TP1 - Normal [cm]
39

Plant Height TP2 - Cold [cm]
40

Plant Height TP2 - Low Nitrogen [cm]
41

Plant Height TP2 - Normal [cm]
42

Plant Height TP2 - 100 mM NaCl [cm]
43

Plant Height TP3 - 100 mM NaCl [cm]
44

Plant Height TP3 - Low Nitrogen [cm]
45

RGR_Leaf_Num_Normal
46

Root Biomass [DW - gr.]/SPAD [100 mM NaCl]
47

Root Biomass [DW - gr.]/SPAD [Cold]
48

Root Biomass [DW - gr.]/SPAD [Low Nitrogen]
49

Root Biomass [DW - gr.]/SPAD [Normal]
50

SPAD - Cold (SPAD unit)
51

SPAD - Low Nitrogen (SPAD unit)
52

SPAD - Normal (SPAD unit)
53

SPAD 100 - mM NaCl (SPAD unit)
54

Shoot Biomass [DW - gr.]/SPAD [100 mM NaCl]
55

Shoot Biomass [DW - gr.]/SPAD [Cold]
56

Shoot Biomass [DW - gr.]/SPAD [Low Nitrogen]
57

Shoot Biomass [DW - gr.]/SPAD [Normal]
58

Total Biomass-Root + Shoot [DW - gr.]/SPAD [100 mM
59

NaCl]

Total Biomass-Root + Shoot [DW - gr.]/SPAD [Cold]
60

Total Biomass-Root + Shoot [DW - gr.]/SPAD [Low
61

Nitrogen]

Total Biomass-Root + Shoot[DW - gr.]/SPAD [Normal]
62

Table 22: Provided are the Sorghum correlated parameters. “N” = nitrogen; Cold conditions = 10 ± 2° C.; NaCl = 100 mM NaCl; Low nitrogen = 1.2 mM Nitrogen; Normal conditions = 16 mM Nitrogen; “TP” = time point. Thus, TP1, TP2 and TP3 refer to time points 1, 2 (TP1 + 8 days) and 3 (TP2 + 7 days), respectively.

TABLE 23

Sorghum accessions, measured parameters under low nitrogen growth conditions

Line/

Corr. ID
3
5
11
15
19
38
41
45
52
21
22

Line-1
0.04
0.08
3.00
4.00
3.90
6.73
13.30
22.23
26.88
27.53
1.87

Line-2
0.11
0.19
3.13
4.58
4.27
9.77
20.63
31.07
28.02
64.12
1.71

Line-3
0.20
0.33
3.87
4.97
4.70
12.70
23.70
34.67
29.64
115.23
1.73

Line-4
0.10
0.16
3.53
4.73
4.23
8.67
18.03
30.03
31.52
58.02
1.57

Line-5
0.08
0.16
3.20
4.60
4.30
9.77
19.33
30.83
29.61
52.22
2.10

Line-6
0.09
0.16
3.13
4.70
4.57
9.23
19.20
29.87
26.82
35.10
1.81

Line-7
0.13
0.26
3.13
4.97
4.63
10.27
21.87
30.87
28.48
84.57
2.06

Line-8
0.09
0.20
3.30
4.87
4.67
10.10
22.13
32.40
28.21
63.73
2.10

Line-9
0.09
0.13
3.07
4.67
3.97
7.93
18.20
29.37
30.48
47.03
1.50

Line-10
0.09
0.18
3.07
4.57
4.10
8.23
21.00
30.70
27.63
60.00
2.00

Table 23: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under low nitrogen conditions. Growth conditions are specified in the experimental procedure section.

TABLE 24

Additional calculated parameters in sorghum accessions, measured

parameters under low nitrogen growth conditions

Line/

Corr. ID
23
24
25
26
27
28
49
57
61

Line-1
9.65
17.88
84.53
81.57
82.58
6.89
0.00
0.00
0.00

Line-2
23.54
40.59
80.95
79.16
79.81
6.57
0.00
0.01
0.01

Line-3
43.88
71.35
117.00
104.75
109.10
6.31
0.01
0.01
0.02

Line-4
22.58
35.44
100.52
103.50
102.32
7.45
0.00
0.01
0.01

Line-5
16.89
35.33
72.54
83.71
79.74
6.89
0.00
0.01
0.01

Line-6
12.44
22.66
71.78
83.22
78.77
5.87
0.00
0.01
0.01

Line-7
28.19
56.38
93.47
107.69
102.49
6.15
0.00
0.01
0.01

Line-8
20.53
43.20
76.05
81.39
79.59
6.05
0.00
0.01
0.01

Line-9
18.76
28.27
86.82
70.30
76.07
7.68
0.00
0.00
0.01

Line-10
20.09
39.91
80.51
75.86
77.36
6.74
0.00
0.01
0.01

Table 24: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under low nitrogen conditions. Growth conditions are specified in the experimental procedure section.

TABLE 25

Sorghum accessions, measured parameters

under salinity growth conditions

Line/Corr. ID
1
6
9
13
17

Line-1
0.05
0.09
3.00
4.00
4.00

Line-2
0.10
0.19
3.13
4.37
4.13

Line-3
0.12
0.20
3.40
4.87
4.57

Line-4
0.07
0.14
3.07
4.60
4.43

Line-5
0.08
0.13
3.33
4.50
4.07

Line-6
0.08
0.13
3.07
4.53
4.33

Line-7
0.14
0.15
3.07
4.50
4.13

Line-8
0.10
0.19
3.27
4.77
4.50

Line-9
0.16
0.10
3.00
4.32
3.78

Line-10
0.14
0.12
3.07
4.20
4.20

Line/Corr. ID
36
43
44
54
29

Line-1
7.90
14.20
21.80
32.73
8.18

Line-2
9.50
16.27
23.17
35.14
8.50

Line-3
10.93
20.37
30.37
27.97
6.12

Line-4
7.93
13.33
22.83
30.93
6.98

Line-5
9.70
15.90
23.70
34.53
8.49

Line-6
8.53
16.53
23.30
29.99
6.92

Line-7
8.90
15.47
22.47
32.09
7.76

Line-8
10.37
18.93
26.83
31.86
7.08

Line-9
7.00
13.68
20.28
32.51
8.60

Line-10
7.83
15.77
23.57
34.32
8.17

Table 25: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under 100 mM NaCl growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 26

Additional calculated parameters in sorghum accessions,

measured parameters under salinity growth conditions

Correlation ID

Line
47
55
59

Line-1
0.002
0.003
0.004

Line-2
0.003
0.005
0.008

Line-3
0.004
0.007
0.012

Line-4
0.002
0.004
0.007

Line-5
0.002
0.004
0.006

Line-6
0.003
0.004
0.007

Line-7
0.004
0.005
0.009

Line-8
0.003
0.006
0.009

Line-9
0.005
0.003
0.008

Line-10
0.004
0.004
0.008

Table 26: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under 100 mM NaCl growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 27

Sorghum accessions, measured parameters under cold growth conditions

Line/

Corre-

lation

ID
2
7
10
14
18
37
40
51
30

Line-1
0.07
0.08
3.00
3.90
4.73
6.50
11.17
28.62
6.05

Line-2
0.11
0.15
3.00
4.13
5.33
8.77
15.87
30.31
5.68

Line-3
0.16
0.19
3.50
4.63
5.43
10.40
18.43
27.04
4.98

Line-4
0.09
0.11
3.17
4.17
5.50
6.80
12.20
32.28
5.87

Line-5
0.08
0.13
3.40
4.27
5.33
9.03
16.03
28.28
5.30

Line-6
0.11
0.16
3.20
4.23
5.07
9.00
14.63
29.89
5.90

Line-7
0.14
0.15
3.13
4.20
4.50
7.97
14.60
32.47
7.21

Line-8
0.13
0.15
3.07
4.30
5.40
9.17
17.27
28.63
5.30

Line-9
0.11
0.11
3.07
4.17
5.37
6.50
13.43
31.71
5.91

Line-10
0.14
0.14
3.00
4.00
5.18
7.23
13.91
29.56
5.70

Table 27: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under cold growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 28

Additional calculated parameters in sorghum accessions,

measured parameters under cold growth conditions

Correlation ID

Line
48
56
60

Line-1
0.002
0.003
0.005

Line-2
0.004
0.005
0.009

Line-3
0.006
0.007
0.013

Line-4
0.003
0.003
0.006

Line-5
0.003
0.005
0.008

Line-6
0.004
0.006
0.009

Line-7
0.004
0.005
0.009

Line-8
0.004
0.005
0.010

Line-9
0.003
0.004
0.007

Line-10
0.005
0.005
0.009

Table 28: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under cold growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 29

Sorghum accessions, measured parameters under regular growth conditions

Line/

Corr. ID
4
8
12
16
20
39
42
46
53
31
32

Line-1
0.05
0.10
3.00
4.17
5.33
7.47
14.97
0.16
26.70
5.01
1.98

Line-2
0.13
0.24
3.07
4.50
5.87
9.30
18.23
0.19
29.33
5.00
1.94

Line-3
0.17
0.31
3.80
4.80
6.20
12.87
22.10
0.16
29.86
4.82
1.90

Line-4
0.10
0.16
3.20
4.60
5.80
8.57
17.60
0.17
29.09
5.02
1.59

Line-5
0.11
0.19
3.23
4.53
5.80
8.93
18.07
0.17
24.98
4.31
1.81

Line-6
0.12
0.19
3.23
4.97
5.73
8.53
18.53
0.17
24.62
4.29
1.58

Line-7
0.14
0.24
3.13
4.60
5.73
10.67
22.83
0.17
30.79
5.37
1.76

Line-8
0.12
0.24
3.43
4.93
6.00
10.27
22.03
0.17
25.50
4.25
1.99

Line-9
0.10
0.19
3.00
4.50
5.60
7.87
20.03
0.17
32.89
5.87
1.89

Line-10
0.11
0.24
3.00
4.57
6.07
8.77
21.80
0.20
33.54
5.53
2.20

Table 29: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under regular growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 30

Additional measured parameters under regular growth conditions

Line/Corr. ID
33
34
35
50
58
62

Line-1
0.86
1.65
2.51
0.002
0.004
0.006

Line-2
2.19
3.87
6.06
0.005
0.008
0.013

Line-3
2.83
5.14
7.96
0.006
0.010
0.016

Line-4
1.69
2.58
4.28
0.004
0.005
0.009

Line-5
1.76
3.18
4.94
0.004
0.008
0.012

Line-6
1.96
3.08
5.04
0.005
0.008
0.012

Line-7
2.27
3.95
6.22
0.005
0.008
0.012

Line-8
2.04
4.00
6.04
0.005
0.010
0.014

Line-9
1.09
2.02
3.11
0.003
0.006
0.009

Line-10
1.88
3.97
5.85
0.003
0.007
0.011

Table 30: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Seed ID) under regular growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 31

Correlation between the expression level of selected LAB genes of some embodiments of the

invention in various tissues and the phenotypic performance under low nitrogen, normal,

cold or salinity stress conditions across Sorghum accessions

Corr.

Gene

Exp.
Set
Gene

Exp.
Corr.

Name
R
P value
set
ID
Name
R
P value
set
Set ID

LAB741
0.94
1.64E−03
3
22
LAB744
0.80
3.18E−02
3
15

LAB744
0.81
2.73E−02
3
45
LAB744
0.77
4.22E−02
3
23

LAB744
0.79
3.41E−02
3
52
LAB744
0.71
7.25E−02
3
28

LAB744
0.74
5.84E−02
3
24
LAB744
0.77
4.36E−02
3
21

LAB744
0.77
1.50E−02
7
1
LAB744
0.74
2.24E−02
8
32

LAB746
0.80
3.09E−02
3
22
LAB746
0.80
1.02E−02
6
22

LAB746
0.76
1.69E−02
2
42
LAB748
0.76
4.61E−02
3
28

LAB748
0.80
1.03E−02
6
27
LAB748
0.90
9.97E−04
6
25

LAB748
0.72
2.72E−02
7
59
LAB748
0.75
2.09E−02
5
56

LAB748
0.74
2.31E−02
5
60
LAB749
0.71
3.09E−02
6
49

LAB749
0.71
3.09E−02
6
3
LAB749
0.71
3.09E−02
6
23

LAB749
0.80
9.00E−03
5
7
LAB749
0.80
1.01E−02
5
48

LAB749
0.87
2.31E−03
5
56
LAB749
0.86
2.99E−03
5
60

LAB749
0.78
1.26E−02
5
37
LAB749
0.80
8.93E−03
5
40

LAB749
0.80
9.46E−03
5
14
LAB750
0.76
4.71E−02
3
25

LAB751
0.71
3.14E−02
6
41
LAB751
0.77
1.58E−02
8
39

LAB751
0.72
2.89E−02
8
4
LAB752
0.77
4.19E−02
3
28

LAB753
0.76
1.65E−02
6
22
LAB753
0.72
2.86E−02
2
35

LAB753
0.73
2.47E−02
2
34
LAB753
0.73
2.55E−02
2
8

LAB753
0.94
1.96E−04
2
20
LAB753
0.81
8.14E−03
2
16

LAB753
0.71
3.06E−02
2
42
LAB753
0.76
1.72E−02
2
58

LAB753
0.72
2.81E−02
2
62
LAB754
0.71
7.23E−02
3
3

LAB754
0.86
1.38E−02
3
15
LAB754
0.74
5.90E−02
3
5

LAB754
0.82
2.28E−02
3
45
LAB754
0.72
7.02E−02
3
41

LAB754
0.84
4.40E−03
7
54
LAB754
0.72
3.00E−02
2
4

LAB755
0.78
1.35E−02
5
30
LAB757
0.71
7.59E−02
3
19

LAB757
0.81
7.93E−03
6
11
LAB757
0.71
3.11E−02
6
19

LAB757
0.74
2.17E−02
7
17
LAB757
0.78
1.28E−02
7
13

LAB757
0.74
2.36E−02
7
47
LAB757
0.75
1.91E−02
2
12

LAB757
0.76
1.86E−02
2
39
LAB757
0.71
3.35E−02
8
12

LAB757
0.71
3.22E−02
8
16
LAB758
0.87
2.41E−03
5
7

LAB758
0.82
6.21E−03
5
56
LAB758
0.79
1.21E−02
5
60

LAB758
0.80
9.37E−03
5
37
LAB758
0.85
3.33E−03
5
40

LAB758
0.79
1.13E−02
5
14
LAB759
0.70
3.53E−02
5
10

LAB762
0.75
2.01E−02
6
22
LAB762
0.72
2.89E−02
5
30

LAB763
0.75
5.26E−02
3
49
LAB763
0.78
3.92E−02
3
5

LAB763
0.84
1.71E−02
3
61
LAB763
0.84
1.70E−02
3
38

LAB763
0.79
3.63E−02
3
19
LAB763
0.86
1.33E−02
3
57

LAB763
0.73
6.31E−02
3
41
LAB763
0.82
6.95E−03
6
49

LAB763
0.79
1.14E−02
6
3
LAB763
0.79
1.21E−02
6
5

LAB763
0.79
1.14E−02
6
23
LAB763
0.81
7.54E−03
6
61

LAB763
0.79
1.21E−02
6
24
LAB763
0.80
1.02E−02
6
21

LAB763
0.70
3.42E−02
6
38
LAB763
0.80
1.03E−02
6
57

LAB763
0.71
3.15E−02
6
41
LAB763
0.73
2.45E−02
5
7

LAB763
0.81
8.02E−03
5
56
LAB763
0.77
1.55E−02
5
60

LAB763
0.77
1.55E−02
5
37
LAB763
0.72
2.84E−02
5
18

LAB763
0.81
8.44E−03
5
40
LAB763
0.83
5.82E−03
5
14

LAB764
0.76
1.64E−02
7
54
LAB769
0.71
3.15E−02
8
42

LAB769
0.73
2.59E−02
8
58
LAB771
0.72
3.04E−02
6
49

LAB771
0.76
1.77E−02
6
27
LAB771
0.72
2.78E−02
6
3

LAB771
0.75
1.93E−02
6
5
LAB771
0.72
2.78E−02
6
23

LAB771
0.73
2.50E−02
6
61
LAB771
0.75
1.93E−02
6
24

LAB771
0.73
2.63E−02
6
26
LAB771
0.75
1.99E−02
6
21

LAB771
0.73
2.63E−02
6
57
LAB771
0.84
4.94E−03
7
1

LAB771
0.82
7.20E−03
7
47
LAB772
0.86
1.38E−02
3
27

LAB772
0.85
1.66E−02
3
11
LAB772
0.85
1.51E−02
3
26

LAB772
0.78
1.23E−02
6
27
LAB772
0.70
3.47E−02
6
25

LAB772
0.78
1.32E−02
6
26
LAB772
0.79
1.04E−02
5
56

LAB772
0.75
1.94E−02
5
60
LAB772
0.84
4.21E−03
5
37

LAB772
0.83
5.77E−03
5
40
LAB772
0.81
7.76E−03
5
14

LAB773
0.82
6.66E−03
5
7
LAB773
0.77
1.42E−02
5
56

LAB773
0.74
2.16E−02
5
60
LAB773
0.78
1.34E−02
5
37

LAB773
0.83
5.74E−03
5
40
LAB773
0.82
6.76E−03
5
14

LAB774
0.77
4.09E−02
3
49
LAB774
0.73
2.48E−02
2
50

LAB774
0.88
1.76E−03
2
12
LAB774
0.79
1.15E−02
2
16

LAB774
0.70
3.41E−02
2
62
LAB776
0.74
2.14E−02
6
28

LAB777
0.80
3.24E−02
3
52
LAB777
0.81
2.72E−02
3
28

LAB777
0.86
2.83E−03
6
52
LAB777
0.84
4.33E−03
5
30

LAB777
0.74
2.31E−02
5
18
LAB778
0.83
2.21E−02
3
22

LAB778
0.86
2.89E−03
2
46
LAB780
0.85
1.46E−02
3
49

LAB780
0.91
4.53E−03
3
3
LAB780
0.78
4.02E−02
3
15

LAB780
0.72
7.08E−02
3
5
LAB780
0.78
3.81E−02
3
45

LAB780
0.88
8.97E−03
3
23
LAB780
0.72
6.92E−02
3
61

LAB780
0.72
7.04E−02
3
24
LAB780
0.80
3.25E−02
3
21

LAB780
0.75
5.30E−02
3
41
LAB780
0.83
5.15E−03
6
49

LAB780
0.81
8.57E−03
6
3
LAB780
0.79
1.13E−02
6
5

LAB780
0.82
6.69E−03
6
45
LAB780
0.81
8.57E−03
6
23

LAB780
0.82
6.70E−03
6
61
LAB780
0.79
1.13E−02
6
24

LAB780
0.81
8.70E−03
6
21
LAB780
0.78
1.27E−02
6
38

LAB780
0.80
1.02E−02
6
57
LAB780
0.82
6.37E−03
6
41

LAB780
0.76
1.75E−02
5
7
LAB780
0.83
5.74E−03
5
56

LAB780
0.78
1.36E−02
5
60
LAB780
0.87
2.49E−03
5
37

LAB780
0.87
2.31E−03
5
40
LAB780
0.82
7.01E−03
5
14

LAB781
0.72
6.80E−02
3
61
LAB781
0.81
2.72E−02
3
19

LAB781
0.75
5.41E−02
3
57
LAB783
0.74
2.38E−02
2
50

LAB783
0.73
2.48E−02
2
20
LAB783
0.74
2.25E−02
2
58

LAB783
0.75
2.01E−02
2
62
LAB783
0.84
4.77E−03
5
10

LAB783
0.75
2.02E−02
5
37
LAB783
0.75
1.22E−02
1
30

LAB785
0.73
2.42E−02
6
5
LAB785
0.73
2.44E−02
6
61

LAB785
0.73
2.42E−02
6
24
LAB785
0.72
2.80E−02
6
21

LAB785
0.74
2.22E−02
6
57
LAB785
0.76
1.65E−02
6
41

LAB785
0.77
1.53E−02
8
42
LAB786
0.83
2.04E−02
3
27

LAB786
0.74
5.64E−02
3
25
LAB786
0.74
5.80E−02
3
26

LAB786
0.71
3.27E−02
6
19
LAB786
0.79
1.13E−02
7
13

LAB789
0.80
1.01E−02
5
10
LAB789
0.72
3.01E−02
5
56

LAB789
0.76
1.86E−02
5
37
LAB789
0.79
1.17E−02
5
40

LAB789
0.80
9.00E−03
5
14
LAB790
0.74
5.87E−02
3
22

LAB791
0.73
2.62E−02
5
7
LAB791
0.70
3.44E−02
5
14

LAB792
0.83
5.79E−03
2
46
LAB793
0.78
1.23E−02
2
50

LAB793
0.74
2.36E−02
2
35
LAB793
0.73
2.59E−02
2
34

LAB793
0.80
1.01E−02
2
8
LAB793
0.81
8.42E−03
2
4

LAB793
0.81
8.61E−03
2
58
LAB793
0.81
8.13E−03
2
62

LAB793
0.73
2.60E−02
2
33
LAB796
0.74
1.50E−02
1
30

LAB797
0.82
2.26E−02
3
11
LAB797
0.80
2.91E−02
3
52

LAB797
0.79
1.10E−02
7
1
LAB797
0.78
1.29E−02
7
47

LAB797
0.85
3.70E−03
5
48
LAB797
0.75
2.01E−02
5
2

LAB797
0.78
1.22E−02
5
56
LAB797
0.84
4.66E−03
5
60

LAB798
0.74
5.62E−02
3
26
LAB798
0.79
1.18E−02
5
7

LAB798
0.75
1.98E−02
5
48
LAB798
0.85
3.89E−03
5
56

LAB798
0.82
6.21E−03
5
60
LAB798
0.76
1.81E−02
5
37

LAB798
0.77
1.47E−02
5
40
LAB799
0.79
3.50E−02
3
49

LAB799
0.81
2.56E−02
3
3
LAB799
0.90
5.60E−03
3
15

LAB799
0.85
1.50E−02
3
45
LAB799
0.74
5.71E−02
3
11

LAB799
0.85
1.52E−02
3
23
LAB799
0.86
1.23E−02
3
52

LAB799
0.74
5.81E−02
3
28
LAB799
0.70
7.86E−02
3
24

LAB799
0.78
4.05E−02
3
21
LAB799
0.79
3.35E−02
3
41

LAB799
0.76
1.64E−02
5
14

Table 31. Correlations (R) between the genes expression evels in various tissues and the phenotypic performance. “Con. ID” - correlation set ID according to the correlated parameters Table above. “Exp. Set” - Expression set. “R” = Pearson correlation coefficient; “P” = p value.

Example 5
Production of Sorghum Transcriptom and High Throughput Correlation Analysis Using 60K Sorghum Oligonucleotide Micro-Array

In order to produce a high throughput correlation analysis between plant phenotype and gene expression level, the present inventors utilized a sorghum oligonucleotide micro-array, produced by Agilent Technologies [Hypertext Transfer Protocol://World Wide Web (dot) chem. (dot) agilent (dot) com/Scripts/PDS (dot) asp?1Page=50879]. The array oligonucleotide represents about 60,000 sorghum genes and transcripts. In order to define correlations between the levels of RNA expression with vigor related parameters, various plant characteristics of 10 different sorghum hybrids were analyzed. The correlation between the RNA levels and the characterized parameters was analyzed using Pearson correlation test [Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot) com/hyperstat/A34739 (dot) html].

Experimental Procedures

Correlation of Sorghum varieties across ecotypes grown in growth chambers under temperature of 30° C. or 14° C. at low light (100 μE) and high light (250 μE) conditions.

Analyzed Sorghum tissues—All 10 selected Sorghum hybrids were sampled per each condition. Leaf tissue growing under 30° C. and low light (100 μE m-²sec-¹), 14° C. and low light (100 μE m-²sec-¹), 30° C. and high light (250 μm-²sec-¹), 14° C. and high light (250 μE m-²sec-¹) were sampled at vegetative stage of four-five leaves and RNA was extracted as described above. Each micro-array expression information tissue type has received a Set ID as summarized in Table 32 below.

TABLE 32

Sorghum transcriptom expression sets in growth chamber experiments

Expression Set
Set ID

Sorghum/leaf: 14 Celsius degree: high light: light on
1

Sorghum/leaf: 14 Celsius degree: low light: light on
2

Sorghum/leaf: 30 Celsius degree: high light: light on
3

Sorghum/leaf: 30 Celsius degree: low light: light on
4

Table 32: Provided are the sorghum transcriptom expression sets.

The following parameters (Table 33) were collected by sampling 8-10 plants per plot or by measuring the parameter across all the plants within the plot.

Relative Growth Rate (RGR) was calculated as regression coefficient of vegetative dry weight along time course.

Leaves number [num]—Plants were characterized for leaf number during growing period. In each measure, plants were measured for their leaf number by counting all the leaves of selected plants per plot.

Shoot fresh weight (FW) [gr.]—shoot fresh weight per plant, measurement of all vegetative tissue above ground.

Shoot dry weight (D id [gr.]—shoot dry weight per plant, measurement of all vegetative tissue above ground after drying at 70° C. in oven for 48 hours.

Leaves temperature [° C.]—leaf temperature was measured using Fluke IR thermometer 568 device. Measurements were done on opened leaves.

Data parameters collected are summarized in Table 33, herein below.

TABLE 33

Sorghum correlated parameters (vectors)

Correlated parameter with
Correlation ID

Leaves number
1

Leaves temperature
2

RGR
3

Shoot DW
4

Shoot FW
5

Table 33. Provided are the Sorghum correlated parameters (vectors).

Experimental Results

10 different Sorghum accessions were grown and characterized for different parameters as described above. Table 33 describes the Sorghum correlated parameters. The average for each of the measured parameters was calculated using the JMP software and values are summarized in Tables 34-37 below. Subsequent correlation analysis between the various transcriptom sets and the average parameters (Table 38) was conducted. Follow, results were integrated to the database.

TABLE 34

Measured parameters in Sorghum accessions under

14° C. and high light (250 μE m−²sec−¹)

Correlation ID

Line
3
4
5

Line-1
0.053
0.037
0.370

Line-2
0.052
0.026
0.253

Line-3
0.034
0.021
0.224

Line-4
0.040
0.023
0.250

Line-5
0.056
0.037
0.431

Line-6
0.061
0.036
0.370

Line-7
0.049
0.022
0.242

Line-8
0.056
0.022
0.226

Line-9
0.068
0.023
0.241

Line-10
0.063
0.027
0.274

Table 34: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Line) under 14° C. and high light (250 μE m−²sec−¹). Growth conditions are specified in the experimental procedure section.

TABLE 35

Measured parameters in Sorghum accessions under 30° C.

and high light (250 μE m−²sec−¹)

Correlation ID

Line
1
3
4
5

Line-1
4.000
0.098
0.076
0.772

Line-2
3.700
0.096
0.050
0.516

Line-3
3.500
0.087
0.047
0.487

Line-4
3.333
0.070
0.036
0.378

Line-5
4.000
0.094
0.065
0.710

Line-6
4.000
0.118
0.085
0.855

Line-7
3.600
0.097
0.049
0.489

Line-8
3.400
0.099
0.042
0.453

Line-9
3.300
0.106
0.042
0.444

Line-10
3.400
0.121
0.062
0.668

Table 35: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Line) under 30° C. and high light (250 μE m−²sec−¹). Growth conditions are specified in the experimental procedure section.

TABLE 36

Measured parameters in Sorghum accessions under 14° C.

and low light (100 μE m−²sec−¹)

Correlation ID

Line
1
3
4
5

Line-1
3
0.032
0.041
0.550

Line-2
3
−0.014
0.013
0.296

Line-3
2.75
−0.022
0.013
0.334

Line-4
2.75
0.024
0.009
0.284

Line-5
2.625
−0.037
0.011
0.364

Line-6
3
−0.045
0.011
0.364

Line-7
3.5
0.083
0.031
0.579

Line-8
2.75
NA
0.009
0.216

Line-9
2.429
−0.050
0.009
0.184

Line-10
2
−0.073
0.009
0.300

Table 36: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Line) under 14° C. and low light (100 μE m−²sec−¹). Growth conditions are specified in the experimental procedure section.

TABLE 37

Measured parameters in Sorghum accessions under 30° C.

and low light (100 μE m−²sec−¹)

Correlation ID

Line
1
2
3
4
5

Line-1
5.273
28.140
0.099
0.114
1.350

Line-2
5.000
29.813
0.098
0.079
1.050

Line-3
4.750
24.213
0.090
0.071
0.884

Line-4
4.000
23.138
0.122
0.056
0.948

Line-5
4.000
19.900
0.108
0.093
1.285

Line-6
4.000
21.350
0.084
0.077
1.126

Line-7
5.250
23.360
0.113
0.040
0.710

Line-8
4.500
29.922
0.121
0.055
0.789

Line-9
3.750
21.525
0.042
0.036
0.665

Line-10
4.000
24.440
0.039
0.050
0.824

Table 37: Provided are the values of each of the parameters (as described above) measured in Sorghum accessions (Line) under 30° C. and low light (100 μE m−²sec−¹). Growth conditions are specified in the experimental procedure section.

TABLE 38

Correlation between the expression level of selected genes of some embodiments of

the invention in various tissues and the phenotypic performance under combinations

of temperature and light conditions treatments (14° C. or 30° C.; high light (250 μE m-²

sec-¹) or low light (100 μE m-²sec-¹) across Sorghum accessions

Corr.

Corr.

Gene

Exp.
Set
Gene

Exp.
Set

Name
R
P value
set
ID
Name
R
P value
set
ID

LAB742
0.80
5.49E−03
1
4
LAB749
0.71
2.14E−02
2
5

LAB750
0.72
1.04E−01
3
4
LAB755
0.75
1.19E−02
2
5

LAB755
0.72
1.07E−01
3
4
LAB756
0.80
5.07E−03
4
4

LAB760
0.86
2.64E−02
3
5
LAB760
0.91
1.15E−02
3
4

LAB760
0.79
6.13E−02
3
1
LAB761
0.75
8.76E−02
3
5

LAB761
0.80
5.80E−02
3
4
LAB765
0.74
1.43E−02
1
4

LAB765
0.83
5.24E−03
2
3
LAB765
0.76
8.25E−02
3
5

LAB765
0.81
4.96E−02
3
4
LAB767
0.72
1.94E−02
4
2

LAB768
0.75
1.26E−02
2
4
LAB770
0.78
8.24E−03
1
5

LAB770
0.79
6.75E−03
1
4
LAB770
0.70
2.37E−02
4
5

LAB770
0.77
9.70E−03
4
4
LAB770
0.73
9.99E−02
3
5

LAB770
0.78
6.47E−02
3
4
LAB774
0.73
9.84E−02
3
5

LAB774
0.79
6.22E−02
3
4
LAB777
0.72
1.07E−01
3
5

LAB777
0.76
7.82E−02
3
4
LAB780
0.74
1.46E−02
1
5

LAB780
0.76
1.11E−02
1
4
LAB780
0.78
7.98E−03
4
3

LAB780
0.86
1.47E−03
2
5
LAB780
0.88
1.93E−03
2
3

LAB780
0.85
2.05E−03
2
4
LAB780
0.74
1.45E−02
2
1

LAB783
0.77
9.40E−03
2
5
LAB783
0.93
2.18E−04
2
3

LAB783
0.73
1.62E−02
2
4
LAB784
0.81
8.69E−03
2
3

LAB784
0.92
1.03E−02
3
5
LAB784
0.88
2.13E−02
3
4

LAB784
0.89
1.76E−02
3
1
LAB787
0.81
7.89E−03
2
3

LAB787
0.71
2.06E−02
2
4
LAB788
0.81
7.45E−03
2
3

LAB789
0.77
1.52E−02
2
3
LAB791
0.79
6.27E−02
3
5

LAB791
0.84
3.63E−02
3
4
LAB792
0.78
6.83E−02
3
3

LAB793
0.77
7.21E−02
3
5
LAB793
0.81
5.14E−02
3
4

LAB794
0.71
1.16E−01
3
5
LAB794
0.76
8.17E−02
3
4

LAB796
0.75
8.37E−02
3
4

Table 38. Correlations (R) between the genes expression levels in various tissues and the phenotypic performance.

“Con. ID”—correlation set ID according to the correlated parameters Table above.

“Exp. Set”—Expression set.

“R” = Pearson correlation coefficient;

“P” = p value.

Example 6

Production of Maize Transcriptom and High Throughput Correlation Analysis with Yield and Nue Related Parameters Using 60K Maize Oligonucleotide Micro-Arrays

In order to produce a high throughput correlation analysis between plant phenotype and gene expression level, the present inventors utilized a maize oligonucleotide micro-array, produced by Agilent Technologies [Hypertext Transfer Protocol://World Wide Web (dot) chem. (dot) agilent (dot) com/Scripts/PDS (dot) asp?1Page=50879]. The array oligonucleotide represents about 60,000 maize genes and transcripts.

Correlation of Maize hybrids across ecotypes grown under low Nitrogen conditions

Experimental Procedures

Twelve Maize hybrids were grown in 3 repetitive plots, in field. Maize seeds were planted and plants were grown in the field using commercial fertilization and irrigation protocols, which included 485 m³water per dunam (1000 square meters) per entire growth period and fertilization of 30 units of URAN® 21% fertilization per dunam per entire growth period (normal conditions). In order to define correlations between the levels of RNA expression with NUE and yield components or vigor related parameters, the 12 different maize hybrids were analyzed. Among them, 11 hybrids encompassing the observed variance were selected for RNA expression analysis. The correlation between the RNA levels and the characterized parameters was analyzed using Pearson correlation test [Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot) com/hyperstat/A34739 (dot) html].

Analyzed Maize tissues—All 11 selected maize hybrids were sampled per each treatment (low N and normal conditions), in three time points: TP2=V6-V8 (six to eight collar leaves are visible, rapid growth phase and kernel row determination begins; TP5=R1-R2 (silking-blister); and TP6=R3-R4 (milk-dough). Four types of plant tissues [Ear, flag leaf indicated in Table as leaf, grain distal part, and internode] were sampled and RNA was extracted as described above. Each micro-array expression information tissue type has received a Set ID as summarized in Tables 39-40 below.

TABLE 39

Maize transcriptom expression sets under low nitrogen conditions

Expression Set
Set ID

Maize field Low N/Ear/TP5
1

Maize field Low N/Ear/TP6
2

Maize field Low N/Internodes/TP2
3

Maize field Low N/Internodes/TP5
4

Maize field Low N/Internodes/TP6
5

Maize field Low N/Leaf/TP2
6

Maize field Low N/Leaf/TP5
7

Maize field Low N/Leaf/TP6
8

Table 39: Provided are the maize transcriptom expression sets under low nitrogen conditions Leaf = the leaf below the main ear; Flower meristem = Apical meristem following male flower initiation; Ear = the female flower at the anthesis day. Grain Distal = maize developing grains from the cob extreme area, Grain Basal = maize developing grains from the cob basal area; Internodes = internodes located above and below the main ear in the plant.

TABLE 40

Maize transcriptom expression sets under normal growth conditions

Expression Set
Set ID

Maize field Normal/Ear/R1-R2
1

Maize field Normal/Grain Distal/R4-R5
2

Maize field Normal/Internode/R3-R4
3

Maize field Normal/Leaf/R1-R2
4

Maize field Normal/Ear/R3-R4
5

Maize field Normal/Internode/R1-R2
6

Maize field Normal/Internode/V6-V8
7

Maize field Normal/Leaf/V6-V8
8

Table 40: Provided are the maize transcriptom expression sets under normal growth conditions. Leaf = the leaf below the main ear; Flower meristem = Apical meristem following male flower initiation; Ear = the female flower at the anthesis day. Grain Distal = maize developing grains from the cob extreme area, Grain Basal = maize developing grains from the cob basal area; Internodes = internodes located above and below the main ear in the plant.

The following parameters were collected using digital imaging system:

Grain Area (cm²)—At the end of the growing period the grains were separated from the ear. A sample of ˜200 grains were weighted, photographed and images were processed using the below described image processing system. The grain area was measured from those images and was divided by the number of grains.

Grain Length and Grain width (cm)—At the end of the growing period the grains were separated from the ear. A sample of ˜200 grains were weighted, photographed and images were processed using the below described image processing system. The sum of grain lengths/or width (longest axis) was measured from those images and was divided by the number of grains.

Ear Area (cm²)—At the end of the growing period 5 ears were photographed and images were processed using the below described image processing system. The Ear area was measured from those images and was divided by the number of Ears.

Ear Length (cm) and Ear Width (mm)—At the end of the growing period 5 ears were photographed and images were processed using the below described image processing system. The Ear length and width (longest axis) was measured from those images and was divided by the number of ears.

The image processing system was used, which consists of a personal desktop computer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ 1.37, Java based image processing software, which was developed at the U.S. National Institutes of Health and is freely available on the internet at Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/. Images were captured in resolution of 10 Mega Pixels (3888×2592 pixels) and stored in a low compression JPEG (Joint Photographic Experts Group standard) format. Next, image processing output data for seed area and seed length was saved to text files and analyzed using the JMP statistical analysis software (SAS institute).

Additional parameters were collected either by sampling 6 plants per plot or by measuring the parameter across all the plants within the plot.

Normalized Grain Weight per plant (kg)—At the end of the experiment all ears from plots within blocks A-C were collected. Six ears were separately threshed and grains were weighted, all additional ears were threshed together and weighted as well. The average grain weight per ear was calculated by dividing the total grain weight by number of total ears per plot (based on plot). In case of 6 ears, the total grains weight of 6 ears was divided by 6.

Ear FW (kg)—At the end of the experiment (when ears were harvested) total and 6 selected ears per plots within blocks A-C were collected separately. The plants with (total and 6) were weighted (gr.) separately and the average ear per plant was calculated for total (Ear FW per plot) and for 6 (Ear FW per plant).

Plant height and Ear height [cm]—Plants were characterized for height at harvesting. In each measure, 6 plants were measured for their height using a measuring tape. Height was measured from ground level to top of the plant below the tassel. Ear height was measured from the ground level to the place were the main ear is located.

Leaf number per plant [num]—Plants were characterized for leaf number during growing period at 5 time points. In each measure, plants were measured for their leaf number by counting all the leaves of 3 selected plants per plot.

Relative Growth Rate was calculated using Formula II (described above).

SPAD [SPAD unit]—Chlorophyll content was determined using a Minolta SPAD 502 chlorophyll meter and measurement was performed at early stages of grain filling (R1-R2) and late stage of grain filling (R3-R4). SPAD meter readings were done on young fully developed leaf. Three measurements per leaf were taken per plot. Data were taken after 46 and 54 days after sowing (DPS).

Dry weight per plant [kg]—At the end of the experiment (when inflorescence were dry) all vegetative material from plots within blocks A-C were collected.

Dry weight=total weight of the vegetative portion above ground (excluding roots) after drying at 70° C. in oven for 48 hours.

Harvest Index (HI) (Maize)—The harvest index per plant was calculated using Formula VIII.

Harvest Index (Maize)=Average grain weight per plant/(Average vegetative dry weight per plant plus Average grain weight per plant) Formula VIII:

Percent Filled Ear [%]—was calculated as the percentage of the Ear area with grains out of the total ear.

Cob diameter [mm]—The diameter of the cob without grains was measured using a ruler.

Kernel Row Number per Ear [num]—The number of rows in each ear was counted.

Experimental Results

Twelve different maize hybrids were grown and characterized for different parameters. Table 41 describes the Maize correlated parameters. The average for each of the measured parameter was calculated using the JMP software (Tables 42-47) and a subsequent correlation analysis was performed (Table 48-49). Results were then integrated to the database.

TABLE 41

Maize correlated parameters (vectors) under low nitrogen conditions

Correlated parameter with
Correlation ID

Ear Length [cm] Low N
1

Ear Length of filled area [cm] Low N
2

Ear width [mm] Low N
3

Final Leaf Number [number] Low N
4

Final Main Ear Height [cm] Low N
5

Final Plant Height [cm] Low N
6

No of rows per ear [number] Low N
7

SPAD at R1-R2 [(SPAD unit)] Low N
8

SPAD at R3-R4 [(SPAD unit)] Low N
9

Stalk width at TP5 Low N
10

Ears weight per plot [kg] Low N
11

Final Plant DW [kg] Low N
12

Final Leaf Area [number] Low N
13

NUE yield kg/N applied in soil kg Low N
14

NUE at early grain filling [R1-R2] yield kg/N in plant
15

per SPAD Low N

NUE at grain filling [R3-R4] yield kg/N in plant per
16

SPAD Low N

NUpE [biomass/N applied] Low N
17

Seed yield per dunam [kg] Low N
18

Yield/LAI Low N
19

Yield/stalk width Low N
20

seed yield per plant [kg] Low N
21

Table 41. “cm” = centimeters' “mm” = millimeters; “kg” = kilograms; SPAD at R1-R2 and SPAD R3-R4: Chlorophyll level after early and late stages of grain filling; “NUE” = nitrogen use efficiency; “NUpE” = nitrogen uptake efficiency; “LAI” = leaf area; “N” = nitrogen; Low N = under low Nitrogen conditions; “Normal” = under normal conditions; “dunam” = 1000 m². “Final” - At harvest.

TABLE 42

Maize correlated parameters (vectors) under normal conditions

Correlated parameter with
Correlation ID

Final Plant DW [kg] Normal
1

Ear Length [cm] Normal
2

Ear Length of filled area [cm] Normal
3

Ear width [mm] Normal
4

Final Leaf Number [number] Normal
5

Final Main Ear Height [cm] Normal
6

Final Plant Height [cm] Normal
7

No of rows per ear [number] Normal
8

SPAD at R1-R2 [(SPAD unit)] Normal
9

SPAD at R3-R4 [(SPAD unit)] Normal
10

Stalk width at TP5 Normal
11

Ears weight per plot [kg] Normal
12

Final Leaf Area [number] Normal
13

NUE yield kg/N applied in soil kg Normal
14

NUE at early grain filling [R1-R2] yield kg/N
15

in plant per SPAD Normal

NUE at grain filling [R3-R4] yield kg/N
16

in plant per SPAD Normal

NUpE [biomass/N applied] Normal
17

Seed yield per dunam [kg] Normal
18

Yield/LAI Normal
19

Yield/stalk width Normal
20

seed yield per plant [kg] Normal
21

Table 42. “cm” = centimeters' “mm” = millimeters; “kg” = kilograms; SPAD at R1-R2 and SPAD R3-R4: Chlorophyll level after early and late stages of grain filling; “NUE” = nitrogen use efficiency; “NUpE” = nitrogen uptake efficiency; “LAI” = leaf area; “N” = nitrogen; Low N = under low Nitrogen conditions; “Normal” = under normal conditions; “dunam” = 1000 m².

TABLE 43

Measured parameters in Maize accessions under Low nitrogen conditions

Corr. ID

Line
1
2
3
4
5
6
7
8
9
10
11

Line-1
20.61
18.40
46.71
15.02
158.08
305.84
14.18
60.24
59.29
2.76
6.61

Line-2
20.98
18.42
48.22
11.64
136.24
270.93
15.21
57.94
57.62
2.42
7.97

Line-3
20.22
19.78
48.32
13.50
128.39
290.61
15.00
58.76
58.40
2.65
9.63

Line-4
20.11
18.83
49.86
11.61
133.06
252.17
15.67
59.48
59.19
2.77
9.22

Line-5
20.11
16.22
52.87
11.83
137.83
260.22
16.00
58.50
58.19
2.67
7.63

Line-6
18.50
16.00
47.44
11.89
99.56
227.22
15.94
64.04
62.67
2.59
7.21

Line-7
19.06
15.28
49.61
12.56
130.17
271.72
15.56
56.42
61.04
2.98
7.92

Line-8
18.25
15.69
48.57
11.67
114.61
248.61
14.50
60.00
59.87
2.61
28.96

Line-9
20.10
16.77
52.41
12.44
143.86
279.33
16.41
58.32
57.47
2.65
7.80

Line-10
17.81
14.06
42.63
9.28
61.61
171.28
14.37
53.06
49.61
2.28
2.41

Line-11
21.25
19.56
50.00
13.17
114.44
269.78
15.74
61.72
61.87
2.82
9.78

Table 43. Provided are the values of each of the parameters (as described above) measured in maize accessions (line) under low nitrogen growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 44

Additional parameters in Maize accessions under Low nitrogen conditions

Corr. ID

Line
12
14
15
16
17
18
20
21
13
19

Line-1
1.59
7.22
18.02
18.35
0.01
1083.75
416.53
0.14
2.92
341.50

Line-2
1.43
8.41
21.79
21.92
0.01
1261.63
528.38
0.16
3.15
408.09

Line-3
1.53
10.33
26.33
26.48
0.01
1549.24
583.46
0.19
3.33
464.77

Line-4
1.95
9.99
25.14
25.33
0.01
1497.86
541.02
0.19
2.87
522.26

Line-5
1.48
7.63
19.55
19.69
0.01
1143.85
428.09
0.14
2.79
439.53

Line-6
1.60
7.73
18.05
18.54
0.01
1159.26
444.29
0.14
3.76
312.58

Line-7
1.58
8.05
21.39
19.78
0.01
1207.42
407.20
0.15
3.50
345.90

Line-8
1.28
8.33
20.79
20.92
0.01
1250.05
477.44
0.16
5.02
287.73

Line-9
1.51
7.64
19.68
19.94
0.01
1146.04
445.60
0.14

Line-10
0.43
2.55
7.21
7.72
0.00
383.22
167.90
0.05

Line-11
1.52
10.60
25.70
25.90
0.01
1589.91
562.29
0.20
3.16
501.24

Table 44. Provided are the values of each of the parameters (as described above) measured in maize accessions (line) under low nitrogen growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 45

Measured parameters in Maize accessions under normal growth conditions

Corr. ID

Line
1
2
3
4
5
6
7
8
9
10
11

Line-1
1.27
19.94
16.23
51.08
11.80
130.31
273.46
16.11
56.89
59.93
2.91

Line-2
1.30
20.17
17.50
46.29
11.11
122.33
260.50
14.67
57.16
60.90
2.64

Line-3
1.33
18.11
17.72
45.92
13.28
127.67
288.00
15.44
59.27
56.89
2.71

Line-4
1.50
19.89
18.44
47.63
11.78
113.02
238.50
15.89
61.61
58.70
2.90

Line-5
1.30
19.50
15.67
51.41
11.94
135.28
286.94
16.17
58.63
58.70
2.70

Line-6
1.58
17.72
14.67
47.42
12.33
94.28
224.83
15.17
61.23
63.16
2.62

Line-7
1.42
17.67
12.94
47.25
12.44
120.94
264.44
16.00
60.17
59.75
2.92

Line-8
1.37
17.28
14.03
46.85
12.22
107.72
251.61
14.83
61.09
62.35
2.72

Line-9
11.38
20.50
18.78
49.28
12.56
112.50
278.44
15.39
62.20
61.93
2.84

Line-10
1.70
17.50
12.33
48.28
11.67
139.67
279.00
17.67
57.51
57.23
2.66

Line-11
0.42
19.86
16.07
41.84
9.28
60.44
163.78
14.27
52.04
49.34
2.26

Table 45. Provided are the values of each of the parameters (as described above) measured in maize accessions (line) under normal growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 46

Additional measured parameters in Maize accessions under normal growth conditions

Corr. ID

Line
12
13
14
15
16
17
18
19
20
21

Line-1
8.94
3.2076
4.4521
23.431
24.978
0.0084
1335.6
426.09
456.71
0.167

Line-2
7.02
3.9473
3.6235
19.052
17.807
0.0087
1087.1
312.97
412.44
0.1359

Line-3
7.53
3.332
4.0084
20.293
20.332
0.0089
1202.5
307.28
443.37
0.1503

Line-4
7.99
4.0116
4.2373
20.719
19.957
0.01
1271.2
362.44
438.7
0.1589

Line-5
8.48
3.864
4.0099
20.486
19.026
0.0087
1203
314.14
446.66
0.1504

Line-6
5.63
4.1908
3.1236
15.36
13.904
0.0106
937.08
224.58
356.95
0.1171

Line-7
6.10
3.9688
3.2863
16.383
16.234
0.0094
985.89
266.44
337.49
0.1232

Line-8
6.66
4.3216
3.5004
17.191
17.214
0.0091
1050.1
261.66
385.79
0.1313

Line-9
8.40
2.8879
4.551
21.955
21.017
0.0759
1365.3
482.33
481.94
0.1707

Line-10
8.21
4.306
4.0869
20.994
21.529
0.0038
1226.1

471.57
0.1533

Line-11
1.88

1.0031
5.7249
5.5186
0.0028
300.93

139.73
0.0376

Table 46. Provided are the values of each of the parameters (as described above) measured in maize accessions (line) under normal growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 47

Correlation between the expression level of selected LAB genes of some

embodiments of the invention in various tissues and the phenotypic

performance under low nitrogen conditions across maize accessions

Gene

Exp.
Corr.
Gene

Exp.
Corr.

Name
R
P value
set
Set ID
Name
R
P value
set
Set ID

LAB673
0.90
5.34E−03
1
18
LAB673
0.96
5.41E−04
1
10

LAB673
0.81
2.56E−02
1
17
LAB673
0.75
5.02E−02
1
9

LAB673
0.87
1.04E−02
1
3
LAB673
0.88
8.49E−03
1
5

LAB673
0.90
5.34E−03
1
14
LAB673
0.94
1.83E−03
1
20

LAB673
0.78
4.06E−02
1
6
LAB673
0.90
5.28E−03
1
15

LAB673
0.81
2.56E−02
1
12
LAB673
0.84
3.68E−02
1
19

LAB673
0.88
8.91E−03
1
2
LAB673
0.83
2.01E−02
1
1

LAB673
0.90
5.34E−03
1
21
LAB673
0.93
2.59E−03
1
16

LAB673
0.83
4.28E−02
6
18
LAB673
0.88
1.96E−02
6
9

LAB673
0.83
4.28E−02
6
14
LAB673
0.83
4.28E−02
6
21

LAB673
0.72
1.05E−01
6
16
LAB673
0.90
2.15E−03
8
4

LAB673
0.75
3.34E−02
8
5
LAB673
0.79
2.05E−02
8
6

LAB673
0.73
6.42E−02
4
17
LAB673
0.83
2.00E−02
4
3

LAB673
0.73
6.42E−02
4
12
LAB675
0.88
9.25E−03
1
10

LAB675
0.90
9.54E−04
5
4
LAB675
0.79
1.11E−02
5
5

LAB675
0.87
2.12E−03
5
6
LAB675
0.79
3.63E−02
8
13

LAB675
0.72
4.19E−02
8
17
LAB675
0.88
3.76E−03
8
11

LAB675
0.72
4.19E−02
8
12
LAB675
0.71
4.74E−02
7
2

LAB675
0.84
1.85E−02
4
8
LAB676
0.98
4.64E−04
6
13

LAB676
0.88
2.03E−02
6
11
LAB676
0.71
7.63E−02
4
8

LAB676
0.77
4.12E−02
4
11
LAB677
0.80
5.69E−02
1
13

LAB677
0.97
1.77E−03
1
19
LAB677
0.72
1.08E−01
6
8

LAB677
0.71
7.34E−02
4
1
LAB678
0.97
3.24E−04
1
10

LAB678
0.78
4.04E−02
1
3
LAB678
0.77
4.09E−02
1
5

LAB678
0.76
4.77E−02
1
6
LAB678
0.86
2.84E−02
6
4

LAB678
0.89
1.72E−02
6
5
LAB678
0.94
5.31E−03
6
6

LAB678
0.83
3.88E−02
6
15
LAB678
0.85
3.04E−02
6
19

LAB678
0.76
7.97E−02
6
2
LAB678
0.80
5.42E−02
6
1

LAB678
0.75
8.28E−02
6
16
LAB678
0.76
2.93E−02
8
1

LAB678
0.79
1.92E−02
7
5
LAB678
0.74
5.88E−02
4
7

LAB679
0.74
9.48E−02
6
17
LAB679
0.84
3.76E−02
6
9

LAB679
0.91
1.16E−02
6
7
LAB679
0.74
9.48E−02
6
12

LAB679
0.72
7.02E−02
2
13
LAB680
0.71
1.16E−01
6
10

LAB680
0.76
7.89E−02
6
9
LAB680
0.71
1.13E−01
6
3

LAB681
0.84
1.78E−02
1
10
LAB681
0.70
7.72E−02
1
2

LAB681
0.90
5.35E−03
1
1
LAB681
0.81
5.31E−02
6
11

LAB681
0.77
7.22E−02
6
1
LAB681
0.78
3.98E−02
8
19

LAB681
0.82
1.20E−02
8
2
LAB681
0.80
1.75E−02
8
1

LAB681
0.83
9.99E−03
2
10
LAB681
0.73
3.86E−02
2
17

LAB681
0.95
2.93E−04
2
9
LAB681
0.76
3.03E−02
2
3

LAB681
0.75
3.14E−02
2
8
LAB681
0.76
2.76E−02
2
7

LAB681
0.73
3.86E−02
2
12
LAB681
0.72
6.94E−02
4
4

LAB681
0.84
1.71E−02
4
6
LAB681
0.72
6.65E−02
4
1

LAB682
0.87
9.98E−03
4
8
LAB683
0.82
2.38E−02
1
18

LAB683
0.75
5.36E−02
1
10
LAB683
0.83
2.14E−02
1
17

LAB683
0.88
8.89E−03
1
3
LAB683
0.94
1.51E−03
1
5

LAB683
0.82
2.38E−02
1
14
LAB683
0.83
2.05E−02
1
20

LAB683
0.78
3.69E−02
1
6
LAB683
0.84
1.88E−02
1
15

LAB683
0.83
2.14E−02
1
12
LAB683
0.79
3.28E−02
1
1

LAB683
0.82
2.38E−02
1
21
LAB683
0.83
2.23E−02
1
16

LAB683
0.93
6.62E−03
6
13
LAB683
0.83
4.08E−02
6
8

LAB683
0.99
1.61E−04
6
11
LAB683
0.73
1.76E−02
3
18

LAB683
0.73
1.76E−02
3
14
LAB683
0.82
3.85E−03
3
20

LAB683
0.72
1.89E−02
3
11
LAB683
0.73
1.76E−02
3
21

LAB683
0.74
1.43E−02
3
16
LAB683
0.79
1.98E−02
8
10

LAB683
0.72
4.59E−02
8
7
LAB683
0.92
1.09E−03
2
17

LAB683
0.78
2.25E−02
2
9
LAB683
0.70
5.18E−02
2
3

LAB683
0.78
2.38E−02
2
5
LAB683
0.92
1.09E−03
2
12

LAB683
0.87
1.04E−02
4
18
LAB683
0.75
5.17E−02
4
3

LAB683
0.87
1.04E−02
4
14
LAB683
0.71
7.54E−02
4
11

LAB683
0.80
3.10E−02
4
15
LAB683
0.72
6.95E−02
4
19

LAB683
0.87
1.04E−02
4
21
LAB683
0.79
3.41E−02
4
16

LAB684
0.73
1.01E−01
6
17
LAB684
0.73
1.01E−01
6
12

LAB684
0.73
6.09E−02
4
5
LAB685
0.77
1.48E−02
5
17

LAB685
0.77
1.48E−02
5
12
LAB685
0.78
6.89E−02
6
6

LAB685
0.72
1.05E−01
6
15
LAB685
0.90
1.56E−02
6
19

LAB685
0.80
5.51E−02
6
2
LAB685
0.95
3.86E−03
6
1

LAB685
0.76
2.86E−02
8
4
LAB685
0.75
3.30E−02
8
5

LAB685
0.76
2.96E−02
8
6
LAB685
0.72
4.49E−02
8
2

LAB685
0.74
3.66E−02
8
1
LAB685
0.73
4.04E−02
7
8

LAB685
0.80
3.02E−02
4
9
LAB685
0.80
3.19E−02
4
4

LAB685
0.96
5.69E−04
4
8
LAB687
0.71
1.15E−01
6
3

LAB687
0.91
1.14E−02
6
5
LAB687
0.85
3.12E−02
6
6

LAB687
0.74
3.43E−02
7
10
LAB687
0.72
4.51E−02
2
18

LAB687
0.87
4.77E−03
2
10
LAB687
0.88
3.80E−03
2
17

LAB687
0.71
4.99E−02
2
9
LAB687
0.82
1.18E−02
2
4

LAB687
0.75
3.28E−02
2
3
LAB687
0.97
6.23E−05
2
5

LAB687
0.72
4.51E−02
2
14
LAB687
0.92
1.30E−03
2
6

LAB687
0.74
3.45E−02
2
15
LAB687
0.88
3.80E−03
2
12

LAB687
0.72
4.51E−02
2
21
LAB687
0.71
4.82E−02
2
16

LAB688
0.71
1.13E−01
6
5
LAB688
0.71
1.16E−01
6
20

LAB688
0.75
8.29E−02
6
2
LAB688
0.84
1.72E−02
4
4

LAB689
0.92
3.25E−03
1
18
LAB689
0.93
2.79E−03
1
10

LAB689
0.75
5.03E−02
1
17
LAB689
0.76
4.88E−02
1
9

LAB689
0.87
1.11E−02
1
4
LAB689
0.89
7.66E−03
1
3

LAB689
0.88
8.19E−03
1
5
LAB689
0.92
3.25E−03
1
14

LAB689
0.91
4.14E−03
1
20
LAB689
0.92
2.84E−03
1
6

LAB689
0.92
3.06E−03
1
15
LAB689
0.75
5.03E−02
1
12

LAB689
0.92
3.25E−03
1
21
LAB689
0.91
4.45E−03
1
16

LAB689
0.71
4.75E−02
5
19
LAB689
0.84
3.45E−02
6
5

LAB689
0.82
3.43E−03
3
18
LAB689
0.72
1.91E−02
3
9

LAB689
0.79
6.52E−03
3
8
LAB689
0.82
3.43E−03
3
14

LAB689
0.80
5.22E−03
3
20
LAB689
0.76
1.12E−02
3
15

LAB689
0.84
2.64E−03
3
2
LAB689
0.82
3.43E−03
3
21

LAB689
0.80
5.92E−03
3
16
LAB689
0.81
1.45E−02
8
3

LAB689
0.73
4.13E−02
8
15
LAB689
0.78
2.38E−02
7
11

LAB689
0.89
3.17E−03
2
18
LAB689
0.82
1.24E−02
2
10

LAB689
0.90
2.67E−03
2
17
LAB689
0.89
3.20E−03
2
9

LAB689
0.92
1.19E−03
2
3
LAB689
0.77
2.51E−02
2
7

LAB689
0.89
3.17E−03
2
14
LAB689
0.85
7.91E−03
2
20

LAB689
0.91
1.96E−03
2
15
LAB689
0.90
2.67E−03
2
12

LAB689
0.89
3.17E−03
2
21
LAB689
0.88
3.92E−03
2
16

LAB689
0.79
3.57E−02
4
5
LAB690
0.92
1.01E−02
6
13

LAB690
0.79
5.88E−02
6
18
LAB690
0.79
5.88E−02
6
14

LAB690
0.85
3.02E−02
6
11
LAB690
0.79
5.88E−02
6
21

LAB690
0.77
4.35E−02
8
19
LAB690
0.76
4.57E−02
2
19

LAB690
0.86
1.20E−02
4
3
LAB691
0.80
3.00E−02
1
4

LAB691
0.73
6.12E−02
1
5
LAB691
0.71
7.55E−02
1
20

LAB691
0.86
1.34E−02
1
6
LAB691
0.75
5.35E−02
1
15

LAB691
0.80
2.93E−02
1
2
LAB691
0.81
2.64E−02
1
1

LAB691
0.73
6.17E−02
1
16
LAB691
0.76
7.95E−02
6
5

LAB691
0.76
8.06E−02
6
6
LAB692
0.83
2.21E−02
1
10

LAB692
0.71
7.23E−02
1
9
LAB692
0.71
3.20E−02
5
10

LAB692
0.87
1.12E−02
7
13
LAB693
0.84
3.73E−02
6
18

LAB693
0.87
2.43E−02
6
4
LAB693
0.84
3.73E−02
6
14

LAB693
0.75
8.46E−02
6
20
LAB693
0.81
5.24E−02
6
6

LAB693
0.77
7.14E−02
6
15
LAB693
0.83
4.32E−02
6
19

LAB693
0.83
3.97E−02
6
2
LAB693
0.84
3.73E−02
6
21

LAB693
0.82
4.78E−02
6
16
LAB693
0.81
2.57E−02
4
9

LAB693
0.78
3.90E−02
4
8
LAB694
0.79
6.36E−02
6
9

LAB694
0.82
4.63E−02
6
8
LAB694
0.80
5.75E−03
3
8

LAB694
0.90
5.82E−03
4
7
LAB695
0.83
1.96E−02
1
1

LAB695
0.80
5.38E−02
6
5
LAB695
0.80
1.81E−02
7
10

LAB695
0.71
5.08E−02
7
9
LAB695
0.72
4.59E−02
7
3

LAB696
0.70
3.39E−02
5
17
LAB696
0.72
2.91E−02
5
7

LAB696
0.70
3.39E−02
5
12
LAB696
0.75
8.57E−02
6
5

LAB696
0.71
4.85E−02
8
7
LAB696
0.83
2.14E−02
8
19

LAB696
0.89
2.70E−03
2
7
LAB696
0.88
8.09E−03
4
8

LAB697
0.73
6.36E−02
1
2
LAB697
0.87
1.06E−02
1
1

LAB697
0.82
4.39E−02
6
20
LAB697
0.86
2.94E−02
6
2

LAB697
0.74
5.70E−02
8
13
LAB697
0.73
6.18E−02
7
13

LAB697
0.78
2.27E−02
7
11
LAB697
0.71
5.01E−02
2
4

LAB697
0.74
3.76E−02
2
6
LAB697
0.76
2.86E−02
2
2

LAB697
0.77
2.45E−02
2
1
LAB697
0.76
4.60E−02
4
10

LAB697
0.86
1.31E−02
4
4
LAB697
0.70
7.86E−02
4
6

LAB698
0.73
1.00E−01
1
13
LAB698
0.84
1.82E−02
1
11

LAB698
0.86
2.93E−03
5
18
LAB698
0.80
1.03E−02
5
10

LAB698
0.84
4.43E−03
5
17
LAB698
0.85
3.41E−03
5
9

LAB698
0.71
3.18E−02
5
4
LAB698
0.90
1.04E−03
5
3

LAB698
0.86
2.93E−03
5
14
LAB698
0.78
1.27E−02
5
20

LAB698
0.78
1.26E−02
5
6
LAB698
0.84
4.27E−03
5
15

LAB698
0.84
4.43E−03
5
12
LAB698
0.73
4.06E−02
5
19

LAB698
0.71
3.33E−02
5
1
LAB698
0.86
2.93E−03
5
21

LAB698
0.83
5.50E−03
5
16
LAB698
0.90
1.53E−02
6
20

LAB698
0.76
7.92E−02
6
2
LAB698
0.75
8.72E−02
6
16

LAB698
0.71
7.11E−02
7
13
LAB698
0.85
6.94E−03
7
11

LAB698
0.71
7.27E−02
4
5
LAB700
0.75
5.23E−02
1
7

LAB700
0.78
3.85E−02
4
8
LAB701
0.95
3.60E−03
1
13

LAB701
0.77
4.25E−02
1
11
LAB701
0.93
6.82E−04
5
13

LAB701
0.74
2.18E−02
5
11
LAB701
0.92
8.33E−03
6
13

LAB701
0.92
8.63E−03
6
11
LAB701
0.92
3.42E−03
8
13

LAB701
0.75
3.13E−02
8
11
LAB701
0.89
7.33E−03
7
13

LAB701
0.86
1.25E−02
2
13
LAB701
0.94
1.60E−03
4
13

LAB701
0.89
6.74E−03
4
11
LAB702
0.76
4.62E−02
1
11

LAB702
0.71
7.34E−02
1
2
LAB702
0.91
4.98E−03
1
1

LAB702
0.78
1.31E−02
5
10
LAB702
0.78
3.95E−02
8
19

LAB702
0.82
1.31E−02
2
10
LAB702
0.93
9.45E−04
2
17

LAB702
0.86
6.76E−03
2
9
LAB702
0.73
3.97E−02
2
3

LAB702
0.73
4.00E−02
2
5
LAB702
0.93
9.45E−04
2
12

LAB703
0.77
1.52E−02
5
10
LAB703
0.70
3.56E−02
5
1

LAB703
0.73
1.01E−01
6
5
LAB703
0.72
4.33E−02
8
10

LAB703
0.71
4.86E−02
7
10
LAB703
0.85
1.46E−02
4
18

LAB703
0.85
1.46E−02
4
14
LAB703
0.79
3.44E−02
4
20

LAB703
0.79
3.34E−02
4
15
LAB703
0.82
2.41E−02
4
19

LAB703
0.79
3.56E−02
4
2
LAB703
0.84
1.88E−02
4
1

LAB703
0.85
1.46E−02
4
21
LAB703
0.80
3.12E−02
4
16

LAB704
0.80
3.02E−02
1
18
LAB704
0.75
5.14E−02
1
5

LAB704
0.80
3.02E−02
1
14
LAB704
0.88
8.67E−03
1
20

LAB704
0.73
6.34E−02
1
6
LAB704
0.76
4.84E−02
1
15

LAB704
0.87
1.16E−02
1
2
LAB704
0.91
4.19E−03
1
1

LAB704
0.80
3.02E−02
1
21
LAB704
0.82
2.48E−02
1
16

LAB704
0.71
3.20E−02
5
1
LAB704
0.82
3.48E−03
3
10

LAB704
0.73
4.00E−02
8
17
LAB704
0.73
4.17E−02
8
5

LAB704
0.73
4.00E−02
8
12
LAB704
0.80
2.97E−02
7
13

LAB704
0.79
1.92E−02
7
5
LAB704
0.86
6.30E−03
7
11

LAB704
0.72
4.31E−02
2
10
LAB704
0.71
4.98E−02
2
5

LAB705
0.71
1.15E−01
6
8
LAB705
0.70
1.21E−01
6
7

LAB705
0.78
3.72E−02
4
9
LAB705
0.89
7.80E−03
4
8

LAB706
0.76
8.02E−02
6
5
LAB706
0.83
1.95E−02
7
13

LAB706
0.71
7.54E−02
4
5
LAB707
0.82
4.64E−02
1
13

LAB708
0.80
5.61E−02
6
5
LAB708
0.77
2.54E−02
2
5

LAB708
0.70
7.97E−02
4
9
LAB708
0.94
1.55E−03
4
8

LAB709
0.93
7.18E−03
1
19
LAB709
0.75
8.48E−02
6
3

LAB709
0.78
3.77E−02
4
13
LAB709
0.81
2.80E−02
4
11

LAB710
0.79
3.37E−02
1
1
LAB710
0.74
9.23E−02
6
18

LAB710
0.85
3.14E−02
6
17
LAB710
0.92
9.62E−03
6
4

LAB710
0.74
9.23E−02
6
14
LAB710
0.75
8.34E−02
6
15

LAB710
0.85
3.14E−02
6
12
LAB710
0.83
3.95E−02
6
19

LAB710
0.74
9.23E−02
6
21
LAB710
0.80
1.74E−02
8
10

LAB710
0.79
2.03E−02
8
4
LAB710
0.80
1.60E−02
8
5

LAB710
0.79
1.88E−02
8
6
LAB710
0.77
4.34E−02
4
6

LAB711
0.87
1.15E−02
1
18
LAB711
0.91
4.87E−03
1
10

LAB711
0.75
5.35E−02
1
17
LAB711
0.90
5.86E−03
1
4

LAB711
0.83
2.04E−02
1
3
LAB711
0.77
4.42E−02
1
5

LAB711
0.87
1.15E−02
1
14
LAB711
0.79
3.29E−02
1
20

LAB711
0.86
1.28E−02
1
6
LAB711
0.89
6.82E−03
1
15

LAB711
0.75
5.35E−02
1
12
LAB711
0.75
5.45E−02
1
2

LAB711
0.87
1.15E−02
1
21
LAB711
0.88
9.53E−03
1
16

LAB711
0.72
1.94E−02
3
17
LAB711
0.75
1.27E−02
3
5

LAB711
0.72
1.94E−02
3
12
LAB711
0.83
3.02E−03
3
1

LAB711
0.77
2.60E−02
2
17
LAB711
0.77
2.60E−02
2
12

LAB712
0.86
2.62E−02
6
20
LAB712
0.75
8.76E−02
6
2

LAB712
0.71
1.15E−01
6
16
LAB712
0.78
4.01E−02
8
13

LAB713
0.73
6.18E−02
1
8
LAB713
0.73
9.89E−02
6
13

LAB713
0.74
9.06E−02
6
8
LAB713
0.87
1.07E−02
2
13

LAB714
0.79
6.21E−02
6
13
LAB714
0.71
1.14E−01
6
9

LAB714
0.73
9.71E−02
6
8
LAB714
0.77
1.58E−02
3
13

LAB714
0.81
2.79E−02
8
13
LAB714
0.92
2.93E−03
7
13

LAB714
0.87
5.53E−03
7
11

Table 47. Correlations (R) between the genes expression levels in various tissues and the phenotypic performance under low nitrogen conditions.

“Corr. ID”—correlation set ID according to the correlated parameters Table above.

“Exp. Set”—Expression set.

“R” = Pearson correlation coefficient;

“P” = p value.

TABLE 48

Correlation between the expression level of selected LAB genes of some

embodiments of the invention in various tissues and the phenotypic

performance under normal conditions across maize accessions

Gene

Exp.
Corr.
Gene

Exp.
Corr.

Name
R
P value
set
Set ID
Name
R
P value
set
Set ID

LAB673
0.72
6.61E−02
1
7
LAB673
0.89
7.29E−03
1
5

LAB673
0.75
5.01E−02
1
9
LAB673
0.75
8.30E−02
5
8

LAB673
0.95
1.03E−03
4
3
LAB673
0.72
6.65E−02
4
8

LAB673
0.84
3.50E−02
4
19
LAB673
0.86
1.29E−02
4
2

LAB673
0.85
1.63E−02
6
7
LAB673
0.87
1.07E−02
6
5

LAB673
0.73
6.10E−02
6
6
LAB675
0.93
6.44E−03
1
13

LAB675
0.90
5.75E−03
1
10
LAB675
0.75
8.29E−02
5
11

LAB675
0.92
1.03E−02
5
8
LAB675
0.83
1.08E−02
2
12

LAB675
0.75
3.37E−02
2
15
LAB675
0.82
1.24E−02
2
14

LAB675
0.71
4.95E−02
2
4
LAB675
0.73
4.10E−02
2
16

LAB675
0.82
1.24E−02
2
18
LAB675
0.75
3.12E−02
2
8

LAB675
0.82
1.24E−02
2
21
LAB675
0.73
4.08E−02
2
20

LAB675
0.70
7.82E−02
4
3
LAB675
0.85
3.29E−02
4
19

LAB675
0.83
1.98E−02
4
2
LAB675
0.73
4.10E−02
3
13

LAB675
0.77
2.61E−02
3
10
LAB676
0.92
9.86E−03
5
10

LAB677
0.94
1.38E−03
1
8
LAB677
0.92
9.95E−03
5
5

LAB677
0.75
3.24E−02
3
5
LAB677
0.77
7.14E−02
6
13

LAB678
0.79
3.63E−02
1
7
LAB678
0.73
6.32E−02
1
12

LAB678
0.83
2.21E−02
1
5
LAB678
0.75
5.26E−02
1
15

LAB678
0.73
6.17E−02
1
14
LAB678
0.75
5.40E−02
1
4

LAB678
0.90
6.39E−03
1
10
LAB678
0.71
7.33E−02
1
16

LAB678
0.85
1.67E−02
1
6
LAB678
0.73
6.17E−02
1
18

LAB678
0.73
6.17E−02
1
21
LAB678
0.71
7.32E−02
1
9

LAB678
0.78
3.74E−02
1
20
LAB678
0.77
7.43E−02
5
11

LAB678
0.75
8.39E−02
5
6
LAB678
0.99
1.09E−04
5
8

LAB678
0.88
4.35E−03
2
12
LAB678
0.70
5.14E−02
2
11

LAB678
0.82
1.22E−02
2
15
LAB678
0.87
5.41E−03
2
14

LAB678
0.79
1.90E−02
2
4
LAB678
0.83
1.00E−02
2
16

LAB678
0.87
5.41E−03
2
18
LAB678
0.85
7.52E−03
2
8

LAB678
0.87
5.41E−03
2
21
LAB678
0.75
3.27E−02
2
19

LAB678
0.81
1.52E−02
2
20
LAB678
0.84
2.18E−03
8
17

LAB678
0.84
2.18E−03
8
1
LAB678
0.87
2.31E−03
8
19

LAB678
0.96
5.76E−04
4
3
LAB678
0.77
7.61E−02
4
19

LAB678
0.71
7.49E−02
4
2
LAB679
0.92
9.44E−03
1
19

LAB679
0.80
3.07E−02
1
2
LAB679
0.87
2.39E−02
5
11

LAB679
0.89
1.87E−02
5
3
LAB679
0.77
7.44E−02
5
20

LAB679
0.92
1.07E−03
2
4
LAB679
0.83
1.13E−02
2
8

LAB679
0.75
3.07E−02
3
11
LAB679
0.72
4.57E−02
3
4

LAB680
0.79
1.96E−02
2
4
LAB680
0.72
4.36E−02
2
6

LAB681
0.85
3.33E−02
5
13
LAB681
0.73
1.01E−01
5
10

LAB681
0.71
1.17E−01
5
8
LAB681
0.83
1.06E−02
2
7

LAB681
0.73
4.00E−02
2
12
LAB681
0.72
4.60E−02
2
4

LAB681
0.85
7.03E−03
2
6
LAB681
0.72
6.69E−02
4
3

LAB681
0.81
2.57E−02
4
2
LAB681
0.71
5.08E−02
3
11

LAB681
0.82
2.44E−02
6
12
LAB681
0.81
2.68E−02
6
11

LAB681
0.82
2.44E−02
6
15
LAB681
0.82
2.47E−02
6
14

LAB681
0.85
1.42E−02
6
4
LAB681
0.73
6.22E−02
6
10

LAB681
0.76
4.74E−02
6
16
LAB681
0.82
2.47E−02
6
18

LAB681
0.82
2.47E−02
6
21
LAB681
0.81
2.75E−02
6
20

LAB681
0.76
1.79E−02
7
4
LAB681
0.79
1.08E−02
7
10

LAB682
0.77
4.20E−02
6
8
LAB683
0.71
1.17E−01
1
13

LAB683
0.79
1.97E−02
2
12
LAB683
0.72
4.61E−02
2
15

LAB683
0.90
2.04E−03
2
4
LAB683
0.99
3.77E−05
4
17

LAB683
0.99
3.77E−05
4
1
LAB683
0.85
3.27E−02
4
19

LAB683
0.87
5.40E−03
3
10
LAB683
0.79
3.37E−02
6
7

LAB683
0.86
1.32E−02
6
12
LAB683
0.77
4.39E−02
6
11

LAB683
0.88
8.97E−03
6
15
LAB683
0.87
1.13E−02
6
14

LAB683
0.81
2.81E−02
6
4
LAB683
0.84
1.89E−02
6
16

LAB683
0.78
3.93E−02
6
6
LAB683
0.87
1.13E−02
6
18

LAB683
0.87
1.13E−02
6
21
LAB683
0.79
6.21E−02
6
19

LAB683
0.88
9.36E−03
6
20
LAB683
0.85
7.72E−03
7
13

LAB683
0.78
1.37E−02
7
10
LAB684
0.89
1.76E−02
1
13

LAB684
0.78
2.36E−02
2
12
LAB684
0.91
1.58E−03
2
4

LAB684
0.90
2.53E−03
2
8
LAB685
0.92
9.61E−03
5
5

LAB685
0.87
2.45E−02
5
3
LAB685
0.71
7.16E−02
4
11

LAB685
0.76
4.81E−02
4
5
LAB685
0.71
7.35E−02
4
14

LAB685
0.90
5.61E−03
4
4
LAB685
0.82
2.25E−02
4
10

LAB685
0.71
7.35E−02
4
18
LAB685
0.75
5.04E−02
4
8

LAB685
0.71
7.35E−02
4
21
LAB685
0.89
6.63E−03
4
9

LAB685
0.71
7.38E−02
4
20
LAB685
0.74
3.40E−02
3
12

LAB685
0.73
3.82E−02
3
15
LAB685
0.70
5.24E−02
3
14

LAB685
0.70
5.24E−02
3
18
LAB685
0.70
5.24E−02
3
21

LAB685
0.75
3.04E−02
3
2
LAB685
0.77
1.42E−02
7
4

LAB685
0.74
2.32E−02
7
8
LAB686
0.72
4.51E−02
2
17

LAB686
0.72
4.51E−02
2
1
LAB687
0.71
7.36E−02
1
17

LAB687
0.71
7.36E−02
1
1
LAB687
0.92
9.97E−03
5
11

LAB687
0.84
3.75E−02
5
8
LAB687
0.84
9.89E−03
2
17

LAB687
0.84
9.89E−03
2
1
LAB687
0.73
1.64E−02
8
7

LAB687
0.74
1.49E−02
8
6
LAB687
0.76
4.61E−02
4
5

LAB687
0.71
7.32E−02
4
15
LAB687
0.73
6.38E−02
4
14

LAB687
0.73
6.38E−02
4
18
LAB687
0.84
1.67E−02
4
8

LAB687
0.73
6.38E−02
4
21
LAB687
0.72
6.58E−02
4
20

LAB687
0.81
2.68E−02
6
10
LAB687
0.72
2.82E−02
7
12

LAB687
0.90
1.01E−03
7
4
LAB688
0.75
8.35E−02
5
5

LAB689
0.86
1.34E−02
1
5
LAB689
0.77
4.41E−02
1
9

LAB689
0.74
9.04E−02
5
10
LAB689
0.71
5.08E−02
2
7

LAB689
0.74
3.46E−02
2
12
LAB689
0.88
3.68E−03
2
4

LAB689
0.76
2.78E−02
2
6
LAB689
0.72
4.42E−02
2
8

LAB689
0.90
3.41E−04
8
11
LAB689
0.70
7.75E−02
4
7

LAB689
0.73
6.36E−02
4
12
LAB689
0.79
3.57E−02
4
17

LAB689
0.73
6.37E−02
4
15
LAB689
0.75
5.01E−02
4
14

LAB689
0.80
3.14E−02
4
3
LAB689
0.74
5.83E−02
4
16

LAB689
0.75
5.01E−02
4
18
LAB689
0.73
6.13E−02
4
8

LAB689
0.75
5.01E−02
4
21
LAB689
0.79
3.57E−02
4
1

LAB689
0.83
3.98E−02
4
19
LAB689
0.73
6.24E−02
4
20

LAB689
0.78
2.21E−02
3
10
LAB689
0.74
5.92E−02
6
5

LAB689
0.83
1.96E−02
6
8
LAB689
0.72
4.51E−02
7
13

LAB689
0.72
2.97E−02
7
5
LAB689
0.72
2.90E−02
7
9

LAB690
0.86
2.71E−02
1
19
LAB690
0.71
1.17E−01
5
7

LAB690
0.91
1.22E−02
5
5
LAB690
0.82
1.24E−02
3
7

LAB690
0.72
1.09E−01
6
13
LAB691
0.82
4.81E−02
5
5

LAB691
0.82
1.17E−02
2
5
LAB691
0.78
3.68E−02
4
6

LAB692
0.83
2.17E−02
1
10
LAB692
0.77
7.09E−02
5
11

LAB692
0.94
5.24E−03
5
8
LAB692
0.80
1.62E−02
2
8

LAB692
0.77
1.42E−02
8
13
LAB692
0.88
2.08E−02
4
13

LAB693
0.75
8.40E−02
5
11
LAB693
0.75
8.78E−02
5
6

LAB693
0.88
2.08E−02
5
8
LAB693
0.84
8.96E−03
2
5

LAB693
0.85
1.89E−03
8
3
LAB693
0.75
2.09E−02
8
19

LAB693
0.83
1.03E−02
3
11
LAB693
0.81
1.38E−02
3
8

LAB693
0.87
2.30E−02
6
13
LAB694
0.76
2.87E−02
2
3

LAB695
0.87
1.12E−02
1
2
LAB695
0.70
1.20E−01
5
16

LAB695
0.82
4.69E−02
5
6
LAB695
0.72
1.07E−01
5
8

LAB695
0.72
2.85E−02
7
4
LAB695
0.72
2.87E−02
7
8

LAB696
0.90
5.25E−03
1
7
LAB696
0.79
3.45E−02
1
12

LAB696
0.94
1.52E−03
1
5
LAB696
0.81
2.84E−02
1
15

LAB696
0.80
3.04E−02
1
14
LAB696
0.74
5.48E−02
1
4

LAB696
0.78
3.78E−02
1
10
LAB696
0.80
2.90E−02
1
16

LAB696
0.83
2.05E−02
1
6
LAB696
0.80
3.04E−02
1
18

LAB696
0.80
3.04E−02
1
21
LAB696
0.76
4.91E−02
1
9

LAB696
0.84
1.68E−02
1
20
LAB696
0.72
1.04E−01
5
17

LAB696
0.72
1.04E−01
5
1
LAB696
0.76
7.96E−02
5
9

LAB696
0.75
3.30E−02
2
4
LAB696
0.76
4.77E−02
6
8

LAB696
0.73
2.46E−02
7
7
LAB696
0.76
1.85E−02
7
12

LAB696
0.70
3.54E−02
7
11
LAB696
0.72
3.01E−02
7
15

LAB696
0.75
2.04E−02
7
14
LAB696
0.86
2.63E−03
7
4

LAB696
0.75
2.04E−02
7
18
LAB696
0.75
1.93E−02
7
8

LAB696
0.75
2.04E−02
7
21
LAB696
0.74
2.21E−02
7
20

LAB697
0.77
4.43E−02
1
17
LAB697
0.77
4.43E−02
1
1

LAB697
0.77
7.19E−02
1
19
LAB697
0.74
9.08E−02
5
17

LAB697
0.74
9.08E−02
5
1
LAB697
0.80
1.71E−02
2
4

LAB697
0.99
2.61E−05
4
17
LAB697
0.99
2.61E−05
4
1

LAB697
0.89
1.60E−02
4
19
LAB697
0.79
3.45E−02
6
7

LAB697
0.76
4.67E−02
6
6
LAB698
0.73
3.97E−02
2
15

LAB698
0.74
3.76E−02
2
14
LAB698
0.74
3.76E−02
2
18

LAB698
0.74
3.76E−02
2
21
LAB698
0.77
2.45E−02
2
20

LAB698
0.80
3.26E−02
4
6
LAB698
0.83
2.03E−02
6
7

LAB698
0.76
4.92E−02
6
12
LAB698
0.92
3.14E−03
6
5

LAB698
0.77
4.23E−02
6
15
LAB698
0.76
4.81E−02
6
14

LAB698
0.72
7.09E−02
6
4
LAB698
0.84
1.77E−02
6
10

LAB698
0.79
3.40E−02
6
16
LAB698
0.85
1.52E−02
6
6

LAB698
0.76
4.81E−02
6
18
LAB698
0.76
4.81E−02
6
21

LAB698
0.79
3.55E−02
6
9
LAB698
0.79
3.50E−02
6
20

LAB700
0.78
2.25E−02
2
4
LAB700
0.79
2.03E−02
2
8

LAB701
0.80
5.62E−02
1
13
LAB701
0.75
3.31E−02
2
5

LAB701
0.92
1.00E−02
6
13
LAB702
0.71
1.17E−01
5
13

LAB702
0.70
1.21E−01
5
10
LAB702
0.84
8.58E−03
2
4

LAB702
0.71
5.08E−02
2
8
LAB702
0.71
4.90E−02
7
13

LAB703
0.71
1.17E−01
5
9
LAB703
0.91
1.65E−03
2
12

LAB703
0.90
2.34E−03
2
15
LAB703
0.90
2.21E−03
2
14

LAB703
0.78
2.26E−02
2
4
LAB703
0.89
3.01E−03
2
16

LAB703
0.90
2.21E−03
2
18
LAB703
0.74
3.76E−02
2
8

LAB703
0.90
2.21E−03
2
21
LAB703
0.89
3.21E−03
2
19

LAB703
0.78
2.24E−02
2
20
LAB703
0.91
5.06E−03
4
17

LAB703
0.70
7.98E−02
4
14
LAB703
0.73
6.45E−02
4
4

LAB703
0.71
7.30E−02
4
3
LAB703
0.70
7.98E−02
4
18

LAB703
0.70
7.98E−02
4
21
LAB703
0.91
5.06E−03
4
1

LAB703
0.95
4.35E−03
4
19
LAB703
0.77
4.34E−02
6
7

LAB703
0.79
3.54E−02
6
12
LAB703
0.82
2.31E−02
6
15

LAB703
0.78
3.81E−02
6
14
LAB703
0.78
3.96E−02
6
16

LAB703
0.86
1.40E−02
6
6
LAB703
0.78
3.81E−02
6
18

LAB703
0.79
3.48E−02
6
8
LAB703
0.78
3.81E−02
6
21

LAB703
0.82
2.47E−02
6
20
LAB704
0.90
5.64E−03
1
7

LAB704
0.85
1.51E−02
1
12
LAB704
0.73
6.22E−02
1
11

LAB704
0.86
1.42E−02
1
5
LAB704
0.88
9.03E−03
1
15

LAB704
0.86
1.28E−02
1
14
LAB704
0.71
7.41E−02
1
4

LAB704
0.87
1.16E−02
1
16
LAB704
0.87
9.95E−03
1
6

LAB704
0.86
1.28E−02
1
18
LAB704
0.70
7.75E−02
1
8

LAB704
0.86
1.28E−02
1
21
LAB704
0.89
7.60E−03
1
20

LAB704
0.84
4.84E−03
8
13
LAB704
0.71
7.62E−02
4
11

LAB704
0.74
3.59E−02
3
2
LAB704
0.75
5.42E−02
6
12

LAB704
0.78
3.74E−02
6
11
LAB704
0.73
6.00E−02
6
15

LAB704
0.73
6.11E−02
6
14
LAB704
0.80
3.11E−02
6
4

LAB704
0.87
1.09E−02
6
10
LAB704
0.73
6.11E−02
6
18

LAB704
0.73
6.11E−02
6
21
LAB704
0.75
5.40E−02
6
9

LAB704
0.74
5.83E−02
6
20
LAB704
0.82
6.64E−03
7
11

LAB704
0.72
2.89E−02
7
15
LAB704
0.72
2.99E−02
7
14

LAB704
0.71
3.11E−02
7
16
LAB704
0.72
2.99E−02
7
18

LAB704
0.72
2.99E−02
7
21
LAB705
0.74
9.41E−02
5
17

LAB705
0.74
9.41E−02
5
1
LAB705
0.90
2.63E−03
2
7

LAB705
0.84
8.58E−03
2
6
LAB705
0.72
4.45E−02
3
17

LAB705
0.72
4.45E−02
3
1
LAB706
0.70
1.19E−01
5
8

LAB706
0.72
4.50E−02
2
4
LAB707
0.71
1.14E−01
5
6

LAB707
0.81
7.79E−03
7
2
LAB708
0.77
7.10E−02
1
19

LAB708
0.87
1.09E−02
1
2
LAB708
0.71
4.62E−02
2
10

LAB708
0.93
7.87E−04
3
3
LAB708
0.77
2.51E−02
3
20

LAB708
0.73
6.08E−02
6
5
LAB708
0.75
5.45E−02
6
10

LAB708
0.71
7.51E−02
6
6
LAB709
0.80
5.42E−02
5
5

LAB709
0.83
4.01E−02
4
19
LAB709
0.79
3.52E−02
4
2

LAB709
0.81
8.58E−03
7
4
LAB709
0.89
1.17E−03
7
8

LAB710
0.78
6.46E−02
5
11
LAB710
0.86
2.77E−02
5
4

LAB710
0.76
1.01E−02
8
17
LAB710
0.76
1.01E−02
8
1

LAB710
0.79
3.36E−02
4
17
LAB710
0.72
6.91E−02
4
8

LAB710
0.79
3.36E−02
4
1
LAB710
0.72
1.09E−01
4
19

LAB710
0.80
3.05E−02
6
3
LAB710
0.76
4.94E−02
6
8

LAB711
0.88
9.36E−03
1
7
LAB711
0.82
2.40E−02
1
12

LAB711
0.77
4.36E−02
1
11
LAB711
0.97
3.29E−04
1
5

LAB711
0.83
2.20E−02
1
15
LAB711
0.83
1.97E−02
1
14

LAB711
0.85
1.43E−02
1
16
LAB711
0.81
2.71E−02
1
6

LAB711
0.83
1.97E−02
1
18
LAB711
0.77
4.41E−02
1
8

LAB711
0.83
1.97E−02
1
21
LAB711
0.76
4.76E−02
1
9

LAB711
0.84
1.71E−02
1
20
LAB711
0.71
1.12E−01
5
11

LAB711
0.83
1.15E−02
2
12
LAB711
0.76
3.01E−02
2
15

LAB711
0.70
5.11E−02
2
14
LAB711
0.94
4.28E−04
2
4

LAB711
0.74
3.48E−02
2
16
LAB711
0.70
5.11E−02
2
18

LAB711
0.70
5.11E−02
2
21
LAB711
0.72
4.50E−02
2
19

LAB711
0.71
3.37E−02
7
8
LAB712
0.76
7.83E−02
5
5

LAB712
0.80
5.38E−02
5
3
LAB712
0.75
5.32E−02
6
5

LAB713
0.92
9.07E−03
5
5
LAB714
0.70
1.19E−01
5
10

LAB714
0.83
3.99E−02
5
9
LAB714
0.78
2.37E−02
2
4

LAB714
0.72
6.72E−02
4
17
LAB714
0.72
6.72E−02
4
1

Table 48. Correlations (R) between the genes expression levels in various tissues and the phenotypic performance under Normal conditions.

“Corr. ID”—correlation set ID according to the correlated parameters Table above.

“Exp. Set”—Expression set.

“R” = Pearson correlation coefficient;

“P” = p value.

Example 7
Production of Maize Transcriptom and High Throughput Correlation Analysis with Yield and Nue Related Parameters Using 44K Maize Oligonucleotide Micro-Arrays

Correlation of Maize Hybrids Across Ecotypes Grown Under Regular Growth Conditions

Experimental Procedures

Twelve Maize hybrids were grown in 3 repetitive plots, in field. Maize seeds were planted and plants were grown in the field using commercial fertilization and irrigation protocols. In order to define correlations between the levels of RNA expression with stress and yield components or vigor related parameters, the 12 different maize hybrids were analyzed. Among them, 10 hybrids encompassing the observed variance were selected for RNA expression analysis. The correlation between the RNA levels and the characterized

parameters was analyzed using Pearson correlation test [Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot) com/hyperstat/A34739 (dot) html].

Analyzed Maize Tissues

All 10 selected maize hybrids were sampled per 3 time points (TP2=V6-V8, TP5=R1-R2, TP6=R3-R4). Four types of plant tissues [Ear, flag leaf (indicated in Table 49 as “leaf”), grain distal part, and internode] growing under normal conditions were sampled and RNA was extracted as described above. Each micro-array expression information tissue type has received a Set ID as summarized in Table 49 below.

TABLE 49

Maize transcriptom expression sets under normal growth conditions

Expression Set
Set ID

Maize field/Normal/Ear TP5
1

Maize field/Normal/Ear TP6
2

Maize field/Normal/Internode TP2
3

Maize field/Normal/Internode TP5
4

Maize field/Normal/Internode TP6
5

Maize field/Normal/Leaf TP2
6

Maize field/Normal/Leaf TP5
7

Maize field/Normal/Grain Distal
8

Table 49: Provided are the maize transcriptom expression sets. Leaf = the leaf below the main ear; Flower meristem = Apical meristem following male flower initiation; Ear = the female flower at the anthesis day. Grain Distal = maize developing grains from the cob extreme area, Grain Basal = maize developing grains from the cob basal area; Internodes = internodes located above and below the main ear in the plant. TP = time point.

The following parameters were collected using digital imaging system:

Ear Area (cm²)—At the end of the growing period 5 ears were, photographed and images were processed using the below described image processing system. The Ear area was measured from those images and was divided by the number of Ears.

Ear Length and Ear Width (cm)—At the end of the growing period 5 ears were, photographed and images were processed using the below described image processing system. The Ear length and width (longest axis) was measured from those images and was divided by the number of ears.

Additional parameters were collected either by sampling 6 plants per plot or by measuring the parameter across all the plants within the plot.

Normalized Grain Weight per plant (gr.)—At the end of the experiment all ears from plots within blocks A-C were collected. Six ears were separately threshed and grains were weighted, all additional ears were threshed together and weighted as well. The average grain weight per ear was calculated by dividing the total grain weight by number of total ears per plot (based on plot). In case of 6 ears, the total grains weight of 6 ears was divided by 6.

Ear FW (gr.)—At the end of the experiment (when ears were harvested) total and 6 selected ears per plots within blocks A-C were collected separately. The plants with (total and 6) were weighted (gr.) separately and the average ear per plant was calculated for total (Ear FW per plot) and for 6 (Ear FW per plant).

Relative Growth Rate was calculated using Formula II (described above).

SPAD [SPAD unit]—Chlorophyll content was determined using a Minolta SPAD 502 chlorophyll meter and measurement was performed 64 days post sowing. SPAD meter readings were done on young fully developed leaf. Three measurements per leaf were taken per plot. Data were taken after 46 and 54 days after sowing (DPS).

Dry weight per plant—At the end of the experiment (when inflorescence were dry) all vegetative material from plots within blocks A-C were collected.

Dry weight=total weight of the vegetative portion above ground (excluding roots) after drying at 70° C. in oven for 48 hours.

Harvest Index (HI) (Maize)—The harvest index was calculated using Formula VIII above.

Percent Filled Ear [%]—was calculated as the percentage of the Ear area with grains out of the total ear.

Cob diameter [mm]—The diameter of the cob without grains was measured using a ruler.

Kernel Row Number per Ear [num]—The number of rows in each ear was counted.

Data parameters collected are summarized in Table 50, herein below

TABLE 50

Maize correlated parameters (vectors)

Correlated parameter with
Correlation ID

Cob Diameter (mm)
1

DW per Plant based on 6 (gr.)
2

Ear Area (cm²)
3

Ear FW per Plant based on 6 (gr.)
4

Ear Height (cm)
5

Ear Length (cm)
6

Ear Width (cm)
7

Ears FW per plant based on all (gr.)
8

Filled per Whole Ear
9

Grain Area (cm²)
10

Grain Length (cm)
11

Grain Width (cm)
12

Growth Rate Leaf Num
13

Kernel Row Number per Ear
14

Leaf Number per Plant
15

Normalized Grain Weight per Plant based on all (gr.)
16

Normalized Grain Weight per plant based on 6 (gr.)
17

Percent Filled Ear
18

Plant Height per Plot (cm)
19

SPAD 46DPS TP2 (SPAD unit)
20

SPAD 54DPS TP5 (SPAD unit)
21

Table 50. SPAD 46DPS and SPAD 54DPS: Chlorophyll level after 46 and 54 days after sowing (DPS). “FW” = fresh weight; “DW” = dry weight. “TP” = Time point.

Experimental Results

Twelve different maize hybrids were grown and characterized for different parameters. The correlated parameters are described in Table 50 above. The average for each of the measured parameter was calculated using the JMP software (Tables 51-52) and a subsequent correlation analysis was performed (Table 53). Results were then integrated to the database.

TABLE 51

Measured parameters in Maize accessions under normal conditions

Corr. ID

Line
1
2
3
4
5
6
7
8
9
10

Line-1
28.96
657.50
85.06
245.83
135.17
19.69
5.58
278.19
0.92
0.75

Line-2
25.08
491.67
85.84
208.33
122.33
19.05
5.15
217.50
0.92
0.71

Line-3
28.05
641.11
90.51
262.22
131.97
20.52
5.67
288.28
0.93
0.75

Line-4
25.73
580.56
95.95
263.89
114.00
21.34
5.53
247.88
0.92
0.77

Line-5
28.72
655.56
91.62
272.22
135.28
20.92
5.73
280.11
0.91
0.81

Line-6
25.78
569.44
72.41
177.78
94.28
18.23
5.23
175.84
0.95
0.71

Line-7
26.43
511.11
74.03
188.89
120.94
19.02
5.22
192.47
0.87
0.71

Line-8
25.19
544.44
76.53
197.22
107.72
18.57
5.33
204.70
0.94
0.75

Line-9

Line-10
26.67
574.17
55.20
141.11
60.44
16.69
4.12
142.72
0.80
0.50

Line-11

522.22
95.36
261.11
112.50
21.70
5.58
264.24
0.96
0.76

Line-12

Table 51. Provided are the values of each of the parameters (as descnbed above) measured in maize accessions (Seed ID) under regular growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 52

Additional measured parameters in Maize accessions under regular growth conditions

Corr. ID

Line
11
12
13
14
15
16
17
18
19
20
21

Line-1
1.17
0.81
0.28
16.17
12.00
153.90
148.68
80.62
278.08
51.67
54.28

Line-2
1.09
0.81
0.22
14.67
11.11
138.88
139.54
86.76
260.50
56.41
57.18

Line-3
1.18
0.80
0.28
16.20
11.69
152.50
153.67
82.14
275.13
53.55
56.01

Line-4
1.20
0.80
0.27
15.89
11.78
159.16
176.98
92.71
238.50
55.21
59.68

Line-5
1.23
0.82
0.31
16.17
11.94
140.46
156.61
80.38
286.94
55.30
54.77

Line-6
1.12
0.80
0.24
15.17
12.33
117.14
119.67
82.76
224.83
59.35
59.14

Line-7
1.14
0.79
0.24
16.00
12.44
123.24
119.69
73.25
264.44
58.48
57.99

Line-8
1.13
0.84
0.27
14.83
12.22
131.27
133.51
81.06
251.61
55.88
60.36

Line-9

52.98
54.77

Line-10
0.92
0.67
0.19
14.27
9.28
40.84
54.32
81.06
163.78
53.86
51.39

Line-11
1.18
0.81
0.30
15.39
12.56
170.66
173.23
91.60
278.44
59.75
61.14

Line-12

49.99
53.34

Table 52. Provided are the values of each of the parameters (as described above) measured in maize accessions (Seed ID) under regular growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 53

Correlation between the expression level of selected LAB genes of some

embodiments of the invention in various tissues and the phenotypic

performance under normal across maize accessions

Gene

Exp.
Corr.
Gene

Exp.
Corr.

Name
R
P value
set
Set ID
Name
R
P value
set
Set ID

AB673
0.70
5.26E−02
5
12
LAB673
0.82
2.38E−02
4
14

AB673
0.70
7.71E−02
4
11
LAB673
0.75
4.98E−02
4
19

AB673
0.78
3.69E−02
4
5
LAB673
0.79
3.65E−02
4
7

AB673
0.80
3.14E−02
4
8
LAB673
0.80
3.04E−02
7
6

AB673
0.79
3.34E−02
7
18
LAB673
0.73
6.14E−02
7
4

AB673
0.84
1.92E−02
1
15
LAB673
0.75
5.18E−02
1
13

AB673
0.75
5.11E−02
1
11
LAB673
0.80
2.90E−02
1
9

AB673
0.82
2.37E−02
1
10
LAB673
0.72
6.77E−02
1
19

AB673
0.80
3.21E−02
1
7
LAB673
0.84
1.91E−02
1
12

AB675
0.72
1.05E−01
4
1
LAB675
0.80
2.92E−02
4
2

AB675
0.77
4.47E−02
1
15
LAB675
0.78
3.93E−02
1
9

AB675
0.81
2.89E−02
1
12
LAB675
0.76
2.91E−02
8
1

AB675
0.73
4.11E−02
8
14
LAB675
0.96
1.59E−04
8
13

AB675
0.89
2.96E−03
8
11
LAB675
0.90
2.31E−03
8
10

AB675
0.87
5.48E−03
8
2
LAB675
0.91
1.68E−03
8
7

AB675
0.77
2.50E−02
8
8
LAB675
0.78
2.12E−02
8
4

AB675
0.78
6.71E−02
2
14
LAB678
0.80
1.68E−02
5
9

AB678
0.81
2.63E−02
4
14
LAB678
0.82
2.54E−02
4
2

AB678
0.76
4.68E−02
7
3
LAB678
0.72
6.79E−02
7
14

AB678
0.78
3.78E−02
7
6
LAB678
0.83
2.18E−02
7
8

AB678
0.83
2.14E−02
7
4
LAB678
0.75
5.26E−02
1
16

AB678
0.87
1.05E−02
1
15
LAB678
0.79
3.27E−02
1
11

AB678
0.93
2.02E−03
1
9
LAB678
0.88
9.20E−03
1
10

AB678
0.84
1.80E−02
1
19
LAB678
0.82
2.38E−02
1
5

AB678
0.83
2.18E−02
1
7
LAB678
0.94
1.53E−03
1
12

AB678
0.71
4.82E−02
8
1
LAB678
0.70
5.30E−02
8
14

AB678
0.80
1.63E−02
8
13
LAB678
0.71
4.73E−02
8
11

AB678
0.72
4.41E−02
8
10
LAB678
0.76
2.91E−02
8
2

AB678
0.71
4.95E−02
8
7
LAB678
0.86
2.90E−02
2
14

AB679
0.83
9.95E−03
5
15
LAB679
0.78
2.32E−02
5
9

AB679
0.98
6.33E−04
2
9
LAB679
0.90
1.55E−02
2
18

AB680
0.70
5.19E−02
5
9
LAB680
0.71
4.72E−02
8
19

AB681
0.93
6.78E−04
5
12
LAB681
0.73
6.51E−02
4
3

AB681
0.71
7.63E−02
4
16
LAB681
0.76
4.88E−02
4
18

AB681
0.75
5.23E−02
4
17
LAB681
0.91
4.93E−03
7
18

AB681
0.78
2.36E−02
8
1
LAB681
0.87
5.40E−03
8
19

AB681
0.83
1.08E−02
8
5
LAB683
0.77
4.10E−02
4
3

AB683
0.79
3.62E−02
4
16
LAB683
0.71
7.38E−02
4
6

AB683
0.73
6.16E−02
4
9
LAB683
0.82
2.36E−02
4
19

AB683
0.71
7.17E−02
4
5
LAB683
0.74
5.81E−02
4
17

AB683
0.74
5.87E−02
7
13
LAB683
0.77
2.50E−02
8
1

AB683
0.75
3.26E−02
8
2
LAB683
0.84
3.81E−02
2
12

AB684
0.80
1.72E−02
8
1
LAB684
0.76
2.73E−02
8
14

AB684
0.81
1.48E−02
8
13
LAB684
0.77
2.40E−02
8
11

AB684
0.72
4.50E−02
8
10
LAB684
0.75
3.32E−02
8
2

AB684
0.72
4.49E−02
8
7
LAB685
0.78
3.70E−02
4
14

AB685
0.73
6.26E−02
7
14
LAB685
0.91
4.64E−03
7
15

AB685
0.82
2.49E−02
7
21
LAB685
0.76
4.96E−02
7
11

AB685
0.87
1.08E−02
7
9
LAB685
0.74
5.47E−02
7
10

AB685
0.73
5.99E−02
7
7
LAB685
0.88
3.57E−03
8
12

AB685
0.71
3.09E−02
3
15
LAB685
0.73
2.47E−02
3
13

AB685
0.78
1.26E−02
3
11
LAB685
0.76
1.70E−02
3
10

AB685
0.71
3.36E−02
3
7
LAB685
0.76
7.86E−02
2
3

AB685
0.76
7.94E−02
2
6
LAB685
0.72
1.07E−01
2
18

AB685
0.80
5.46E−02
2
17
LAB686
0.72
6.98E−02
4
18

AB686
0.81
2.56E−02
7
18
LAB687
0.74
5.60E−02
4
9

AB687
0.80
3.16E−02
7
3
LAB687
0.82
2.44E−02
7
16

AB687
0.90
5.18E−03
7
14
LAB687
0.71
7.21E−02
7
15

AB687
0.86
1.35E−02
7
13
LAB687
0.84
1.71E−02
7
11

AB687
0.84
1.74E−02
7
6
LAB687
0.70
7.95E−02
7
9

AB687
0.76
4.89E−02
7
10
LAB687
0.75
5.07E−02
7
19

AB687
0.71
7.26E−02
7
5
LAB687
0.86
1.21E−02
7
7

AB687
0.86
1.26E−02
7
8
LAB687
0.85
1.45E−02
7
4

AB687
0.80
3.22E−02
7
17
LAB687
0.71
7.42E−02
1
9

AB687
0.71
2.04E−02
6
19
LAB687
0.73
1.68E−02
6
5

AB688
0.74
9.31E−02
2
2
LAB689
0.81
2.69E−02
4
14

AB689
0.73
6.14E−02
4
13
LAB689
0.85
1.47E−02
7
3

AB689
0.83
1.99E−02
7
16
LAB689
0.83
2.09E−02
7
14

AB689
0.93
2.64E−03
7
13
LAB689
0.84
1.75E−02
7
11

AB689
0.95
8.86E−04
7
6
LAB689
0.74
5.88E−02
7
10

AB689
0.73
6.19E−02
7
18
LAB689
0.81
2.74E−02
7
7

AB689
0.86
1.40E−02
7
8
LAB689
0.92
2.92E−03
7
4

AB689
0.86
1.26E−02
7
17
LAB689
0.78
3.88E−02
1
15

AB689
0.79
3.50E−02
1
13
LAB689
0.71
7.34E−02
1
11

AB689
0.73
6.44E−02
1
9
LAB689
0.85
7.73E−03
8
1

AB689
0.76
2.85E−02
8
13
LAB689
0.84
8.89E−03
8
19

AB689
0.72
4.34E−02
8
2
LAB689
0.79
1.89E−02
8
5

AB689
0.74
1.41E−02
6
15
LAB689
0.76
1.80E−02
3
15

AB689
0.73
2.65E−02
3
11
LAB689
0.76
1.70E−02
3
10

AB689
0.77
1.60E−02
3
12
LAB689
0.91
1.07E−02
2
15

AB690
0.83
4.19E−02
4
1
LAB690
0.77
4.45E−02
4
2

AB691
0.81
2.86E−02
7
5
LAB691
0.71
7.38E−02
1
5

AB692
0.73
6.08E−02
4
14
LAB692
0.86
1.21E−02
4
2

AB692
0.71
7.48E−02
1
9
LAB692
0.77
4.34E−02
1
12

AB692
0.74
3.65E−02
8
13
LAB692
0.78
2.21E−02
8
11

AB692
0.83
4.06E−02
2
14
LAB693
0.78
2.12E−02
5
14

AB693
0.72
4.54E−02
5
11
LAB693
0.79
6.08E−03
6
18

AB693
0.79
6.32E−02
2
14
LAB693
0.71
1.15E−01
2
5

AB694
0.82
1.17E−02
8
3
LAB694
0.71
4.85E−02
8
6

AB694
0.71
4.91E−02
8
17
LAB694
0.74
9.12E−02
2
18

AB695
0.78
6.89E−02
4
1
LAB695
0.84
1.88E−02
1
18

AB695
0.76
3.00E−02
8
2
LAB695
0.72
1.06E−01
2
3

AB695
0.87
2.47E−02
2
14
LAB695
0.85
3.13E−02
2
11

AB695
0.71
1.15E−01
2
6
LAB695
0.74
9.11E−02
2
19

AB695
0.78
6.51E−02
2
5
LAB695
0.79
5.99E−02
2
7

AB695
0.83
3.88E−02
2
8
LAB695
0.81
5.10E−02
2
4

AB696
0.79
3.52E−02
1
3
LAB696
0.86
1.33E−02
1
16

AB696
0.74
5.61E−02
1
15
LAB696
0.75
5.33E−02
1
11

AB696
0.87
1.09E−02
1
9
LAB696
0.82
2.39E−02
1
10

AB696
0.94
1.63E−03
1
19
LAB696
0.87
1.01E−02
1
5

AB696
0.82
2.45E−02
1
7
LAB696
0.71
7.13E−02
1
8

AB696
0.85
1.63E−02
1
12
LAB696
0.79
3.47E−02
1
17

AB696
0.71
3.19E−02
3
16
LAB696
0.83
5.84E−03
3
15

AB696
0.83
6.09E−03
3
11
LAB696
0.70
3.43E−02
3
6

AB696
0.79
1.06E−02
3
10
LAB696
0.78
1.24E−02
3
19

AB696
0.75
2.03E−02
3
7
LAB696
0.71
3.28E−02
3
12

AB696
0.72
2.76E−02
3
17
LAB696
0.72
1.04E−01
2
9

AB697
0.80
1.69E−02
5
11
LAB697
0.73
3.88E−02
5
6

AB697
0.75
3.27E−02
5
17
LAB697
0.78
6.69E−02
4
1

AB697
0.73
6.11E−02
4
19
LAB697
0.72
6.59E−02
4
5

AB697
0.82
2.44E−02
4
8
LAB697
0.91
1.22E−02
1
1

AB697
0.79
3.64E−02
1
8
LAB697
0.75
3.05E−02
8
1

AB698
0.76
4.53E−02
4
14
LAB698
0.79
3.42E−02
4
15

AB698
0.79
3.28E−02
4
11
LAB698
0.84
1.83E−02
4
9

AB698
0.82
2.44E−02
4
10
LAB698
0.75
5.46E−02
4
19

AB698
0.83
2.17E−02
4
5
LAB698
0.82
2.30E−02
4
7

AB698
0.70
7.92E−02
4
8
LAB698
0.81
2.72E−02
4
12

AB698
0.81
2.74E−02
7
5
LAB698
0.77
7.48E−02
1
1

AB698
0.78
3.71E−02
1
2
LAB698
0.80
1.77E−02
8
1

AB698
0.91
1.53E−03
8
2
LAB698
0.82
1.30E−02
8
7

AB698
0.84
8.86E−03
8
8
LAB698
0.74
3.43E−02
8
4

AB698
0.85
3.18E−02
2
12
LAB702
0.77
2.48E−02
5
9

AB702
0.71
1.12E−01
7
1
LAB702
0.82
4.67E−02
1
1

AB702
0.75
5.44E−02
1
2
LAB702
0.79
2.02E−02
8
1

AB703
0.79
3.42E−02
4
3
LAB703
0.79
3.31E−02
4
16

AB703
0.70
7.83E−02
4
13
LAB703
0.82
2.26E−02
4
6

AB703
0.74
5.91E−02
4
9
LAB703
0.73
6.26E−02
4
10

AB703
0.78
3.97E−02
4
18
LAB703
0.83
2.11E−02
4
19

AB703
0.87
1.09E−02
4
5
LAB703
0.74
5.85E−02
4
7

AB703
0.74
5.91E−02
4
12
LAB703
0.78
3.81E−02
4
4

AB703
0.76
4.97E−02
4
17
LAB703
0.74
5.77E−02
7
3

AB703
0.71
7.54E−02
7
16
LAB703
0.77
4.27E−02
7
13

AB703
0.86
1.35E−02
7
6
LAB703
0.79
3.57E−02
7
18

AB703
0.75
5.08E−02
7
4
LAB703
0.72
6.71E−02
7
17

AB703
0.73
3.94E−02
8
1
LAB703
0.86
5.62E−03
8
16

AB703
0.77
2.67E−02
8
14
LAB703
0.71
4.89E−02
8
6

AB703
0.76
2.78E−02
8
2
LAB703
0.77
2.63E−02
8
7

AB703
0.80
1.71E−02
8
8
LAB703
0.78
2.34E−02
8
4

AB703
0.83
4.09E−02
2
9
LAB703
0.79
6.01E−02
2
18

AB703
0.94
5.91E−03
2
12
LAB704
0.72
6.72E−02
4
16

AB704
0.77
4.09E−02
4
9
LAB704
0.75
5.30E−02
4
10

AB704
0.85
1.53E−02
4
12
LAB704
0.71
7.50E−02
4
17

AB704
0.72
6.69E−02
7
11
LAB704
0.71
7.42E−02
7
10

AB704
0.84
1.78E−02
1
3
LAB704
0.87
1.15E−02
1
16

AB704
0.77
4.24E−02
1
14
LAB704
0.71
7.25E−02
1
15

AB704
0.71
7.49E−02
1
13
LAB704
0.83
2.04E−02
1
11

AB704
0.77
4.08E−02
1
6
LAB704
0.80
3.09E−02
1
9

AB704
0.81
2.67E−02
1
10
LAB704
0.87
1.09E−02
1
19

AB704
0.87
1.08E−02
1
5
LAB704
0.88
8.76E−03
1
7

AB704
0.84
1.75E−02
1
8
LAB704
0.71
7.11E−02
1
12

AB704
0.80
2.96E−02
1
4
LAB704
0.82
2.29E−02
1
17

AB704
0.87
2.35E−02
2
15
LAB705
0.88
4.25E−03
8
19

AB705
0.82
1.36E−02
8
5
LAB705
0.74
9.55E−02
2
9

AB705
0.79
6.12E−02
2
18
LAB706
0.79
1.87E−02
8
1

AB706
0.75
3.10E−02
8
13
LAB706
0.71
4.99E−02
8
11

AB706
0.88
4.05E−03
8
2
LAB706
0.75
3.05E−02
8
7

AB707
0.96
1.90E−03
2
14
LAB707
0.87
2.36E−02
2
5

AB708
0.74
3.65E−02
5
3
LAB708
0.78
2.27E−02
5
18

AB708
0.71
7.26E−02
4
16
LAB708
0.78
4.06E−02
4
14

AB708
0.73
6.42E−02
4
15
LAB708
0.75
5.39E−02
4
11

AB708
0.87
1.18E−02
4
9
LAB708
0.77
4.46E−02
4
10

AB708
0.83
2.07E−02
4
19
LAB708
0.81
2.66E−02
4
5

AB708
0.75
5.28E−02
4
7
LAB708
0.85
1.50E−02
4
12

AB708
0.76
7.82E−02
2
12
LAB709
0.75
3.15E−02
8
15

AB710
0.95
3.94E−03
4
1
LAB710
0.72
6.60E−02
4
14

AB710
0.79
3.59E−02
7
14
LAB710
0.81
2.88E−02
7
13

AB710
0.82
2.31E−02
7
6
LAB710
0.77
4.40E−02
7
4

AB710
0.76
1.01E−02
6
20
LAB711
0.83
2.15E−02
1
3

AB711
0.89
7.80E−03
1
16
LAB711
0.89
7.10E−03
1
14

AB711
0.78
3.75E−02
1
15
LAB711
0.84
1.90E−02
1
13

AB711
0.93
2.75E−03
1
11
LAB711
0.78
3.86E−02
1
6

AB711
0.81
2.79E−02
1
9
LAB711
0.90
5.67E−03
1
10

AB711
0.87
1.17E−02
1
19
LAB711
0.89
7.09E−03
1
5

AB711
0.96
5.30E−04
1
7
LAB711
0.88
8.72E−03
1
8

AB711
0.78
3.83E−02
1
12
LAB711
0.85
1.63E−02
1
4

AB711
0.85
1.51E−02
1
17
LAB711
0.82
1.22E−02
8
1

AB711
0.71
5.07E−02
8
13
LAB711
0.76
3.03E−02
8
2

AB712
0.83
1.17E−02
5
9
LAB712
0.78
6.81E−02
7
1

AB712
0.73
1.03E−01
2
7
LAB713
0.85
3.12E−02
1
1

AB714
0.84
9.02E−03
8
1
LAB714
0.74
3.69E−02
8
19

AB714
0.96
2.07E−03
2
12

Table 53. Correlations (R) between the genes expression levels in various tissues and the phenotypic performance under normal conditions.

“Corr. ID”—correlation set ID according to the correlated parameters Table above.

“Exp. Set”—Expression set.

“R” = Pearson correlation coefficient;

“P” = p value.

Example 8
Production of Maize Transcriptom and High Throughput Correlation Analysis with Yield, Nue, and Abst Related Parameters Measured in Semi-Hydroponics Conditions Using 60K Maize Oligonucleotide Micro-Arrays

Maize vigor related parameters under low nitrogen (1.6 mM), salinity (100 mM NaCl), low temperature (10±2° C.) and normal growth conditions—Twelve Maize hybrids were grown in 5 repetitive plots, each containing 7 plants, at a net house under semi-hydroponics conditions. Briefly, the growing protocol was as follows: Maize seeds were sown in trays filled with a mix of vermiculite and peat in a 1:1 ratio. Following germination, the trays were transferred to the high salinity solution (100 mM NaCl in addition to the Full Hoagland solution), low temperature (10±2° C. in the presence of Full Hoagland solution), low nitrogen solution (the amount of total nitrogen was reduced in 90% from the full Hoagland solution, i.e., to a final concentration of 10% from full Hoagland solution, final amount of 1.6 mM N) or at Normal growth solution (Full Hoagland containing 16 mM N solution, at 28±2° C.). Plants were grown at 28±2° C. unless otherwise indicated.

Full Hoagland solution consists of: KNO₃—0.808 grams/liter, MgSO₄—0.12 grams/liter, KH₂PO₄—0.136 grams/liter and 0.01% (volume/volume) of ‘Super coratin’ micro elements (Iron-EDDHA [ethylenediamine-N,N′-bis(2-hydroxyphenylacetic acid)]—40.5 grams/liter; Mn—20.2 grams/liter; Zn 10.1 grams/liter; Co 1.5 grams/liter; and Mo 1.1 grams/liter), solution's pH should be 6.5-6.8.

Experimental Procedures

Analyzed Maize tissues—Twelve selected Maize hybrids were sampled per each treatment. Two tissues [leaves and root tip] representing different plant characteristics were sampled. Plants were sampled from all 4 treatments applied: salinity (100 mM NaCl), low temperature (10±2° C.), low Nitrogen (1.6 mM N) and Normal conditions. Sampling was done at the vegetative stage (V4-5) and RNA was extracted as described above. Each micro-array expression information tissue type has received a Set ID as summarized in Table 54-57 below.

TABLE 54

Maize transcriptom expression sets under normal

conditions at semi hydroponics system

Expression set
Set ID

maize/leaf: Normal
1

maize/root tip: Normal
2

Table 54: Provided are the Maize transcriptom expression sets at normal conditions.

TABLE 55

Maize transcriptom expression sets under cold

conditions at semi hydroponics system

Expression set
Set ID

maize/leaf: Cold
1

maize/root tip: Cold
2

Table 55: Provided are the Maize transcriptom expression sets at cold conditions.

TABLE 56

Maize transcriptom expression sets under low nitrogen

conditions at semi hydroponics system

Expression set
Set ID

maize/leaf: 1.6 mM N
1

maize/root tip: 1.6 mM N
2

Table 56: Provided are the Maize transcriptom expression sets at low nitrogen conditions 1.6 Mm Nitrogen.

TABLE 57

Maize transcriptom expression sets under high

salinity conditions at semi hydroponics system

Expression set
Set ID

maize/leaf: NaCl 100 mM
1

maize/root tip: NaCl 100 mM
2

Table 57: Provided are the Maize transcriptom expression sets at 100 mM NaCl.

Phenotypic Parameters Assessment

Ten different Maize hybrids were grown and characterized at the vegetative stage (V4-5) for the following parameters:

Leaves dry weight (DW)=leaves dry weight per plant (average of five plants); “Plant Height growth”=was calculated as regression coefficient of plant height [cm] along time course (average of five plants);

Root dry weight (DW)—root dry weight per plant, all vegetative tissue above ground (average of four plants);

Shoot dry weight (DW)—shoot dry weight per plant, all vegetative tissue above ground (average of four plants) after drying at 70° C. in oven for 48 hours;

Shoot fresh weight (FW)—shoot fresh weight per plant, all vegetative tissue above ground (average of four plants);

SPAD [SPAD unit]—Chlorophyll content was determined using a Minolta SPAD 502 chlorophyll meter and measurement was performed 30 days post sowing. SPAD meter readings were done on young fully developed leaf. Three measurements per leaf were taken per plot.

Plant height growth—the relative growth rate (RGR) of Plant Height was calculated using Formula IX:

Relative growth rate of Plant height=Regression coefficient of Plant height along time course (measured in cm/day). Formula IX:

Root length—the length of the root was measured at V4 developmental stage.

Data parameters collected are summarized in Tables 58-59, herein below

TABLE 58

Maize correlated parameters (vectors) under cold conditions

Correlated parameter with
Correlation ID

Leaves DW
1

Plant height growth
2

Root DW
3

SPAD (SPAD unit)
4

Shoot DW
5

Shoot FW
6

Table 58: Provided are the Maize correlated parameters under cold conditions. “DW” = dry weight; “FW” = fresh weight; “SPAD” = chlorophyll levels.

TABLE 59

Maize correlated parameters (vectors) under normal,

low nitrogen and salinity growth conditions

Correlated parameter with
Correlation ID

Leaves DW
1

Plant height growth
2

Root DW
3

Root length
4

SPAD (SPAD unit)
5

Shoot DW
6

Shoot FW
7

Table 59: Provided are the Maize correlated parameters under normal, low nitrogen and salinity growth conditions. “DW” = dry weight; “FW” = fresh weight; “SPAD” = chlorophyll levels.

Experimental Results

Twelve different maize accessions were grown and characterized for different parameters as described above. Tables 58-59 describes the maize correlated parameters. The average for each of the measured parameter was calculated using the JMP software and values are summarized in Tables 60-63 below. Subsequent correlation analysis between the various transcriptom sets and the average parameters (Tables 64-67) was conducted. Follow, results were integrated to the database.

TABLE 60

Maize accessions, measured parameters under low

nitrogen growth conditions

Correlation ID

Line
1
2
3
4
5
6
7

Line-1
0.57
0.75
0.38
44.50
21.43
2.56
23.27

Line-2
0.45
0.81
0.35
45.63
21.24
1.96
20.58

Line-3
0.46
0.88
0.25
44.25
22.23
2.01
19.26

Line-4
0.48
0.69
0.36
43.59
24.56
1.94
20.02

Line-5
0.36
0.83
0.31
40.67
22.75
1.94
17.98

Line-6
0.51
0.84
0.30
42.03
26.47
2.52
22.06

Line-7
0.53
0.78
0.29
42.65
22.08
2.03
21.28

Line-8
0.58
0.92
0.31
45.06
25.09
2.37
22.13

Line-9
0.55
0.89
0.29
45.31
23.73
2.09
20.29

Line-10
0.51
0.85
0.32
42.17
25.68
2.17
19.94

Line-11
0.56
0.80
0.43
41.03
25.02
2.62
22.50

Line-12
0.39
0.64
0.17
37.65
19.51
1.53
15.93

Table 60: Provided are the values of each of the parameters (as described above) measured in Maize accessions (Line) under low nitrogen conditions. Growth conditions are specified in the experimental procedure section.

TABLE 61

Maize accessions, measured parameters under

100 mM NaCl growth conditions

Correlation ID

Line
1
2
3
4
5
6
7

Line-1
0.41
0.46
0.05
10.88
36.55
2.43
19.58

Line-2
0.50
0.40
0.05
11.28
39.92
2.19
20.78

Line-3
0.43
0.45
0.03
11.82
37.82
2.25
18.45

Line-4
0.48
0.32
0.07
10.08
41.33
2.26
19.35

Line-5
0.43
0.32
0.05
8.46
40.82
1.54
15.65

Line-6
0.56
0.31
0.03
10.56
44.40
1.94
16.09

Line-7
0.33
0.29
0.10
10.14
37.92
1.78
12.46

Line-8
0.51
0.36
0.06
11.83
43.22
1.90
16.92

Line-9
0.47
0.37
0.02
10.55
39.83
1.89
16.75

Line-10
0.98
0.35
0.04
11.18
38.20
2.20
17.64

Line-11
0.48
0.31
0.05
10.09
38.14
1.86
15.90

Line-12
0.15
0.27
0.01
8.90
37.84
0.97
9.40

Table 61: Provided are the values of each of the parameters (as described above) measured in Maize accessions (Line) under 100 mM NaCl growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 62

Maize accessions, measured parameters under

cold growth conditions

Correlation ID

Line
1
2
3
4
5
6

Line-1
1.19
2.15
0.05
28.88
5.74
73.79

Line-2
1.17
1.93
0.07
29.11
4.86
55.46

Line-3
1.02
2.12
0.10
27.08
3.98
53.26

Line-4
1.18
1.80
0.08
32.38
4.22
54.92

Line-5
1.04
2.32
0.07
32.68
4.63
58.95

Line-6
1.23
2.15
0.07
32.89
4.93
62.36

Line-7
1.13
2.49
0.14
31.58
4.82
63.65

Line-8
0.98
2.01
0.07
33.01
4.03
54.90

Line-9
0.88
1.95
0.07
28.65
3.57
48.25

Line-10
1.28
2.03
0.02
31.43
3.99
52.83

Line-11
1.10
1.85
0.05
30.64
4.64
55.08

Line-12
0.60
1.21
0.06
30.71
1.89
29.61

Table 62: Provided are the values of each of the parameters (as described above) measured in Maize accessions (Line) under cold growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 63

Maize accessions, measured parameters under

regular growth conditions

Correlation ID

Line
1
2
3
4
5
6
7

Line-1
1.16
1.99
0.14
20.15
34.50
5.27
79.00

Line-2
1.10
1.92
0.11
15.89
35.77
4.67
62.85

Line-3
0.92
1.93
0.23
18.59
34.70
3.88
59.73

Line-4
1.01
1.93
0.16
18.72
34.42
5.08
63.92

Line-5
0.93
2.15
0.08
16.38
35.26
4.10
60.06

Line-6
0.91
1.95
0.05
14.93
37.52
4.46
64.67

Line-7
1.11
2.23
0.17
17.48
36.50
4.68
68.10

Line-8
1.01
1.94
0.10
15.74
36.07
4.59
65.81

Line-9
1.01
1.97
0.07
15.71
33.74
4.08
58.31

Line-10
1.02
2.05
0.10
17.58
34.34
4.61
61.87

Line-11
1.23
1.74
0.14
16.13
35.74
5.42
70.04

Line-12
0.44
1.26
0.03
17.43
29.04
2.02
35.96

Table 63: Provided are the values of each of the parameters (as described above) measured in Maize accessions (Line) under regular growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 64

Correlation between the expression level of selected genes of some embodiments of

the invention in various tissues and the phenotypic performance under normal

conditions across Maize accessions

Corr.

Corr.

Gene

Exp.
Set

Exp.
Set

Name
R
P value
set
ID
Gene Name
R
P value
set
ID

LAB686
0.71
2.03E−02
1
1
LAB686
0.78
7.90E−03
1
6

LAB689
0.74
2.41E−02
2
7
LAB689
0.71
3.05E−02
2
1

LAB689
0.72
2.84E−02
2
3
LAB689
0.78
1.34E−02
2
6

LAB700
0.71
3.36E−02
2
1
LAB700
0.72
2.77E−02
2
6

LAB701
0.81
4.52E−03
1
4

Table 64. Correlations (R) between the genes expression levels in various tissues and the phenotypic performance.

“Corr. ID”—correlation set ID according to the correlated parameters Table above.

“Exp. Set”—Expression set.

“R” = Pearson correlation coefficient;

“P” = p value.

TABLE 65

Correlation between the expression level of selected genes of some embodiments of

the invention in various tissues and the phenotypic performance under low

nitrogen conditions across Maize accessions

Corr.

Corr.

Exp.
Set

Exp.
Set

Gene Name
R
P value
set
ID
Gene Name
R
P value
set
ID

LAB682
0.74
1.47E−02
1
1
LAB683
0.78
7.75E−03
1
1

LAB683
0.74
1.43E−02
1
6
LAB686
0.79
6.17E−03
1
3

LAB687
0.76
1.77E−02
2
5
LAB687
0.79
1.18E−02
2
3

LAB688
0.72
2.96E−02
2
1
LAB688
0.76
1.04E−02
1
6

LAB689
0.93
3.13E−04
2
5
LAB690
0.70
3.43E−02
2
5

LAB697
0.84
5.01E−03
2
5
LAB710
0.71
3.16E−02
2
4

Table 65. Correlations (R) between the genes expression levels in various tissues and the phenotypic performance.

“Corr. ID”—correlation set ID according to the correlated parameters Table above.

“Exp. Set”—Expression set.

“R” = Pearson correlation coefficient;

“P” = p value.

TABLE 66

Correlation between the expression level of selected genes of some embodiments of

the invention in various tissues and the phenotypic performance under cold

conditions across Maize accessions

Corr.

Corr.

Exp.
Set

Exp.
Set

Gene Name
R
P value
set
ID
Gene Name
R
P value
set
ID

LAB681
0.83
5.54E−03
2
4
LAB683
0.80
1.79E−02
1
3

LAB686
0.78
2.34E−02
1
6
LAB686
0.75
3.23E−02
1
2

LAB686
0.75
3.08E−02
1
1
LAB686
0.77
2.56E−02
1
5

LAB687
0.81
1.48E−02
1
3
LAB687
0.79
1.10E−02
2
1

LAB691
0.73
2.47E−02
2
4
LAB700
0.72
2.99E−02
2
5

LAB706
0.71
3.10E−02
2
6
LAB713
0.78
2.33E−02
1
3

Table 66. Correlations (R) between the genes expression levels in various tissues and the phenotypic performance.

“Corr. ID”—correlation set ID according to the correlated parameters Table above.

“Exp. Set”—Expression set.

“R” = Pearson correlation coefficient;

“P” = p value.

TABLE 67

Correlation between the expression level of selected genes of some embodiments of

the invention in various tissues and the phenotypic performance under

salinity conditions across Maize accessions

Corr.

Corr.

Exp.
Set

Exp.
Set

Gene Name
R
P value
set
ID
Gene Name
R
P value
set
ID

LAB675
0.76
1.69E−02
2
3
LAB676
0.71
2.21E−02
1
1

LAB683
0.72
2.01E−02
1
2
LAB691
0.71
3.30E−02
2
5

LAB703
0.80
1.02E−02
2
5
LAB704
0.86
1.31E−03
1
1

LAB710
0.89
5.71E−04
1
5
LAB712
0.70
3.49E−02
2
5

Table 67. Correlations (R) between the genes expression levels in various tissues and the phenotypic performance.

“Corr. ID”—correlation set ID according to the correlated parameters Table above.

“Exp. Set”—Expression set.

“R” = Pearson correlation coefficient;

“P” = p value.

Example 9
Production of Maize Transcriptom and High Throughput Correlation Analysis when Grown Under Normal and Defoliation Conditions Using 60K Maize Oligonucleotide Micro-Array

To produce a high throughput correlation analysis, the present inventors utilized a Maize oligonucleotide micro-array, produced by Agilent Technologies [Hypertext Transfer Protocol://World Wide Web (dot) chem. (dot) agilent (dot) com/Scripts/PDS (dot) asp?1Page=50879]. The array oligonucleotide represents about 60K Maize genes and transcripts designed based on data from Public databases (Example 1). To define correlations between the levels of RNA expression and yield, biomass components or vigor related parameters, various plant characteristics of 13 different Maize hybrids were analyzed under normal and defoliation conditions. Same hybrids were subjected to RNA expression analysis. The correlation between the RNA levels and the characterized parameters was analyzed using Pearson correlation test [Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot) com/hyperstat/A34739 (dot) html].

Experimental Procedures

Thirteen maize hybrids lines were grown in 6 repetitive plots, in field. Maize seeds were planted and plants were grown in the field using commercial fertilization and irrigation protocols. After silking, 3 plots in every hybrid line underwent the defoliation treatment. In this treatment all the leaves above the ear were removed. After the treatment all the plants were grown according to the same commercial fertilization and irrigation protocols.

Three tissues at flowering (R1) and grain filling (R3) developmental stages including leaf (flowering—R1), stem (flowering—R1 and grain filling -R3), and flowering meristem (flowering—R1) representing different plant characteristics, were sampled from treated and untreated plants. RNA was extracted as described in “GENERAL EXPERIMENTAL AND BIOINFORMATICS METHODS”. For convenience, each micro-array expression information tissue type has received a Set ID as summarized in Tables 68-69 below.

TABLE 68

Tissues used for Maize transcriptom expression

sets (Under normal conditions)

Expression Set
Set ID

Ear at flowering stage under normal conditions
1

leaf at flowering stage under normal conditions
2

stem at flowering stage under normal conditions
3

stem at grain filling stage under normal conditions
4

Table 68. Provided are the identification (ID) number of each of the Maize transcriptome expression sets.

TABLE 69

Tissues used for Maize transcriptom expression

sets (Under defoliation conditions)

Expression Set
Set ID

Ear at flowering stage under defoliation conditions
1

leaf at flowering stage under defoliation conditions
2

stem at flowering stage under defoliation conditions
3

stem at grain filling stage under defoliation conditions
4

Table 69 Provided are the identification (ID) number of each of the Maize transcriptome expression sets.

The following parameters were collected by imaging.

Institutes of Health and is freely available on the internet at Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/. Images were captured in resolution of 10 Mega Pixels (3888×2592 pixels) and stored in a low compression JPEG (Joint Photographic Experts Group standard) format. Next, image processing output data for seed area and seed length was saved to text files and analyzed using the JMP statistical analysis software (SAS institute).

1000 grain weight (gr.)—At the end of the experiment all seeds from all plots were collected and weighed and the weight of 1000 was calculated.

Ear Area (cm²)—At the end of the growing period Sears were, photographed and images were processed using the below described image processing system. The Ear area was measured from those images and was divided by the number of ears.

Ear Length and Ear Width (cm)—At the end of the growing period 6 ears were, photographed and images were processed using the below described image processing system. The Ear length and width (longest axis) was measured from those images and was divided by the number of ears.

Grain Area (cm²)—At the end of the growing period the grains were separated from the ear. A sample of ˜200 grains were weight, photographed and images were processed using the below described image processing system. The grain area was measured from those images and was divided by the number of grains.

Grain Length and Grain width (cm)—At the end of the growing period the grains were separated from the ear. A sample of ˜200 grains were weight, photographed and images were processed using the below described image processing system. The sum of grain lengths/or width (longest axis) was measured from those images and was divided by the number of grains.

Grain Perimeter (cm)—At the end of the growing period the grains were separated from the ear. A sample of ˜200 grains were weight, photographed and images were processed using the below described image processing system. The sum of grain perimeter was measured from those images and was divided by the number of grains.

Ear filled grain area (cm²)—At the end of the growing period 5 ears were photographed and images were processed using the below described image processing system. The Ear area filled with kernels was measured from those images and was divided by the number of Ears.

Filled per Whole Ear—was calculated as the length of the ear with grains out of the total ear.

Additional parameters were collected either by sampling 6 plants per plot (SP) or by measuring the parameter across all the plants within the plot.

Cob width [mm]—The diameter of the cob without grains was measured using a ruler.

Ear average (avr.) weight [gr.]—At the end of the experiment (when ears were harvested) total and 6 selected ears per plots were collected. The ears were weighted and the average ear per plant was calculated. The ear weight was normalized using the relative humidity to be 0%.

Plant height and Ear height (cm)—Plants were characterized for height at harvesting. In each measure, 6 plants were measured for their height using a measuring tape. Height was measured from ground level to top of the plant below the tassel. Ear height was measured from the ground level to the place were the main ear is located.

Ear row number (num)—The number of rows per ear was counted.

Ear fresh weight per plant (GF)—During the grain filling period (GF) and total and 6 selected ears per plot were collected separately. The ears were weighted and the average ear weight per plant was calculated.

Ears dry weight (Kg)—At the end of the experiment (when ears were harvested) total and 6 selected ears per plots were collected and weighted. The ear weight was normalized using the relative humidity to be 0%.

Ears fresh weight (Kg)—At the end of the experiment (when ears were harvested) total and 6 selected ears per plots were collected and weighted.

Ears per plant (num)—the number of ears per plant was counted.

Grains yield (Kg.)—At the end of the experiment all ears were collected. Ears from 6 plants from each plot were separately threshed and grains were weighted.

Grains dry yield (Kg.)—At the end of the experiment all ears were collected. Ears from 6 plants from each plot were separately threshed and grains were weighted. The grain weight was normalized using the relative humidity to be 0%.

Grain yield per ear (Kg.)—At the end of the experiment all ears were collected. 5 ears from each plot were separately threshed and grains were weighted. The average grain weight per ear was calculated by dividing the total grain weight by the number of ears.

Leaves area cm²) per plant (GF, and HD) [LAI]=Total leaf area of 6 plants in a plot. This parameter was measured using a Leaf area-meter at two time points during the course of the experiment: at heading (HD) and during the grain filling period (GF).

Leaves fresh weight (gr.) (GF, and HD)—This parameter was measured at two time points during the course of the experiment: at heading (HD) and during the grain filling period (GF). Leaves used for measurement of the LAI were weighted.

Lower stem fresh weight (gr.) (GF, HD, and H)—This parameter was measured at three time points during the course of the experiment: at heading (HD), during the grain filling period (GF) and at harvest (H). Lower internodes from at least 4 plants per plot were separated from the plant and weighted. The average internode weight per plant was calculated by dividing the total grain weight by the number of plants.

Lower stem length (cm) (GF, HD, and H)—This parameter was measured at three time points during the course of the experiment: at heading (HD), during the grain filling period (GF) and at harvest (H). Lower internodes from at least 4 plants per plot were separated from the plant and their length was measured using a ruler. The average internode length per plant was calculated by dividing the total grain weight by the number of plants.

Lower stem width (mm) (GF, HD, and H)—This parameter was measured at three time points during the course of the experiment: at heading (HD), during the grain filling period (GF) and at harvest (H). Lower internodes from at least 4 plants per plot were separated from the plant and their diameter was measured using a caliber. The average internode width per plant was calculated by dividing the total grain weight by the number of plants.

Plant height growth—the relative growth rate (RGR) of Plant Height was calculated using Formula IX above, by Regression coefficient of Plant height along time course, measured in cm/day).

SPAD [SPAD unit]—Chlorophyll content was determined using a Minolta SPAD 502 chlorophyll meter and measurement was performed 64 days post sowing. SPAD meter readings were done on young fully developed leaf. Three measurements per leaf were taken per plot. Data were taken after 46 and 54 days after sowing (DPS).

Stem fresh weight (gr.) (GF and HD)—This parameter was measured at two time points during the course of the experiment: at heading (HD) and during the grain filling period (GF). Stems of the plants used for measurement of the LAI were weighted.

Total dry matter (kg)—Total dry matter was calculated as follows:

Normalized ear weight per plant+vegetative dry weight. Formula X:

Upper stem fresh weight (gr.) (GF, HD and H)—This parameter was measured at three time points during the course of the experiment: at heading (HD), during the grain filling period (GF) and at harvest (H). Upper internodes from at least 4 plants per plot were separated from the plant and weighted. The average internode weight per plant was calculated by dividing the total grain weight by the number of plants.

Upper stem length (cm) (GF, HD and H)—This parameter was measured at three time points during the course of the experiment: at heading (HD), during the grain filling period (GF) and at harvest (H). Upper internodes from at least 4 plants per plot were separated from the plant and their length was measured using a ruler.

Upper stem width (mm) (GF, HD and H)—This parameter was measured at three time points during the course of the experiment: at heading (HD), during the grain filling period (GF) and at harvest (H). Upper internodes from at least 4 plants per plot were separated from the plant and their diameter was measured using a caliber.

Vegetative dry weight (Kg.)—total weight of the vegetative portion of 6 plants (above ground excluding roots) after drying at 70° C. in oven for 48 hours divided by the number of plants.

Vegetative fresh weight (Kg.)—total weight of the vegetative portion of 6 plants (above ground excluding roots).

Node number—nodes on the stem were counted at the heading stage of plant development.

TABLE 70

Maize correlated parameters (vectors) under normal conditions and under defoliation

Normal conditions
Defoliation

Correlated parameter with
Correlation ID
Correlated parameter with
Correlation ID

1000 grains weight (gr.)
1
1000 grains weight (gr.)
1

Cob width (mm)
2
Cob width (mm)
2

Ear Area (cm²)
3
Ear Area (cm²)
3

Ear Filled Grain Area (cm²)
4
Ear Filled Grain Area (cm²)
4

Ear Width (cm)
5
Ear Width (cm)
5

Ear avr. Weight (gr)
6
Ear avr weight (gr)
6

Ear height (cm)
7
Ear height (cm)
7

Ear length (feret's diameter) (cm)
8
Ear length (feret's diameter) (cm)
8

Ear row num (num)
9
Ear row num (num)
9

Ears FW per plant (GF) (gr/plant)
10
Ears dry weight (SP)
10

Ears dry weight (SP) (kg)
11
Ears fresh weight (SP) (kg)
11

Ears fresh weight (SP) (kg)
12
Ears per plant (SP) (num)
12

Ears per plant (SP) (num)
13
Filled/Whole Ear
13

Filled/Whole Ear
14
Grain Perimeter (cm)
14

Grain Perimeter (cm)
15
Grain area (cm²)
15

Grain area (cm²)
16
Grain length (cm)
16

Grain length (cm)
17
Grains dry yield (SP) (kg)
17

Grain width (cm)
18
Grains yield (SP) (kg)
18

Grains dry yield (SP) (kg)
19
Grains yield per ear (SP) (kg)
20

Grains yield (SP) (kg)
20
Leaves FW (HD) (gr)
21

Grains yield per ear (SP) (kg)
21
Leaves area PP (HD) (cm²)
22

Leaves FW (GF) (gr)
22
Lower Stem FW (H)(gr)
23

Leaves FW (HD) (gr)
23
Lower Stem FW (HD) (gr)
23

Leaves area PP (GF) (cm²)
24
Lower Stem length (H) (cm)
24

Leaves area PP (HD) (cm²)
25
Lower Stem length (HD) (cm)
25

Leaves temperature (GF) (° c.)
26
Lower Stem width (H) (mm)
26

Lower Stem FW (GF) (gr)
27
Lower Stem width (HD) (mm)
27

Lower Stem FW (H) (gr)
28
Node number (num)
28

Lower Stem FW (HD) (gr)
29
Plant height (cm)
29

Lower Stem length (GF) (cm)
30
Plant height growth (cm/day)
30

Lower Stem length (H) (cm)
31
SPAD (GF) (SPAD unit)
31

Lower Stem length (HD) (cm)
32
Stem FW (HD)(gr)
32

Lower Stem width (GF) (mm)
33
Total dry matter (SP) (kg)
33

Lower Stem width (H) (mm)
34
Upper Stem FW (H) (gr)
34

Lower Stem width (HD) (mm)
35
Upper Stem length (H) (cm)
35

Node number (num)
36
Upper Stem width (H) (mm)
36

Plant height (cm)
37
Vegetative DW (SP) (kg)
37

Plant height growth (cm/day)
38
Vegetative FW (SP) (kg)
38

SPAD (GF) (SPAD unit)
39

Stem FW (GF)(gr)
40

Stem FW (HD)(gr)
41

Total dry matter (SP) (kg)
42

Upper Stem FW (GF) (gr)
43

Upper Stem FW (H)(gr)
44

Upper Stem length (GF) (cm)
45

Upper Stem length (H) (cm)
46

Upper Stem width (GF) (mm)
47

Upper Stem width (H) (mm)
48

Vegetative DW (SP) (kg)
49

Vegetative FW (SP) (kg)
50

Table 70. Provided are the maize correlated parameters (vectors). “SPAD” = chlorophyll levels; “FW” = Plant Fresh weight; “DW” = Plant Dry weight; “GF” = grain filling growth stage; “HD” = heading stage; “H” = harvest stage. “SP” = selected 6 plants for phenotyping.

Thirteen maize varieties were grown, and characterized for parameters as described above. The average for each parameter was calculated using the JMP software, and values are summarized in Tables 71-74 below. Subsequent correlation between the various transcriptom sets for all or sub set of lines was done by the bioinformatic unit and results were integrated into the database (Tables 75 and 76 below).

TABLE 71

Measured parameters in Maize Hybrid under normal conditions

Ecotype/

Treatment
Line-1
Line-2
Line-3
Line-4
Line-5
Line-6

1
296.50251
263.24954
303.61446
304.69706
281.18264
330.45207

2
24.63333
25.10583
23.20667
23.68889
22.81083
22.40167

3
82.29635
74.62558
76.99696
90.15090
83.80019
96.62837

4
80.88746
72.41504
73.43049
85.96127
80.64266
95.03413

5
4.65586
4.78698
4.96127
4.99750
4.64984
4.80226

6
209.50000
164.62698
177.44444
218.52778
205.58333
135.77149

7
121.66667
134.23529
149.63889
152.13889
143.83333
133.64706

8
22.09059
19.62220
20.02428
23.20903
22.62695
23.73754

9
13.00000
14.94444
14.55556
14.55556
13.55556
13.05882

10
351.26167
323.07727
307.87417
330.60000
320.51333
434.59583

11
1.25700
1.08733
1.06467
1.31117
1.23350
1.35350

12
1.68667
1.45667
1.41200
1.69933
1.51933
1.73933

13
1.00000
1.11111
1.00000
1.00000
1.00000
1.05556

14
0.98232
0.96921
0.95254
0.95283
0.94929
0.93737

15
3.29898
3.23282
3.27539
3.33768
3.17788
3.38166

16
0.72008
0.66656
0.70570
0.72205
0.67116
0.75337

17
1.12471
1.12272
1.13333
1.16981
1.08080
1.15931

18
0.80820
0.75252
0.78920
0.78174
0.78711
0.82253

19
0.90706
0.79995
0.76552
0.92313
0.83336
0.98626

20
1.03690
0.91277
0.86947
1.05777
0.95347
1.12280

21
0.15118
0.13333
0.12759
0.15386
0.13889
0.16438

22
230.12917
197.63636
201.03083
205.52500
224.81250
204.48636

23
110.96750
80.57000
157.21000
128.83250
100.57250
111.80000

24
7034.59600
6402.79500
6353.07400
6443.92300
6835.49583
6507.33333

25
4341.25000
3171.00000
4205.50000
4347.50000
3527.00000
4517.33333

26
33.11111
33.51668
33.86852
34.17593
33.77963
32.85187

27
35.40333
25.02545
26.51417
21.74333
26.12500
34.44417

28
23.51722
20.34000
25.08333
14.17889
17.53056
25.73556

29
72.98750
59.90000
74.71500
90.47500
69.52000
66.91000

30
19.35000
20.40000
20.92500
21.37500
20.03333
20.30833

31
16.76111
20.02222
22.59444
21.67778
22.34444
21.39444

32
14.50000
17.75000
20.00000
19.35000
20.33333
20.75000

33
19.85500
16.84091
16.13917
16.36917
17.01167
17.52500

34
19.42333
17.18778
16.08611
16.92000
17.51667
17.87611

35
24.13750
20.53250
20.97250
24.43000
21.70000
19.49250

36
15.22222
14.55556
14.61111
14.83333
15.00000
13.83333

37
265.11111
255.94444
271.11111
283.88889
279.72222
268.77778

38
5.42547
5.58918
6.14928
5.98862
6.36719
6.47215

39
59.77222
53.17037
53.20555
54.94814
53.99073
55.23889

40
649.02583
489.31818
524.05500
512.65833
542.15667
627.75833

41
758.61000
587.87500
801.32000
794.80000
721.86500
708.38000

42
2.56533
2.05800
2.31600
2.44183
2.36117
2.56683

43
19.61417
15.53909
17.82417
10.79250
14.41333
20.31000

44
12.93667
11.21222
12.97500
6.50222
7.98500
12.08111

45
16.63333
18.75455
18.37500
17.91667
17.60000
18.79167

46
16.92778
18.75556
18.71667
20.01111
19.40000
19.65000

47
15.99583
14.10636
13.50333
11.88917
13.08167
14.33917

48
14.93333
12.99778
12.44444
12.04000
12.88667
13.28389

49
1.30833
0.97067
1.25133
1.13067
1.12767
1.21333

50
3.15667
2.25200
2.60733
2.59600
2.41600
2.64000

Table 71. Provided are the values of each of the parameters (as described above) measured in Maize accessions (line) under normal growth conditions. Growth conditions are specified in the experimental procedure section

TABLE 72

Measured parameters in Maize Hybrid under normal conditions, additional maize lines

Ecotype/

Treatment
Line-7
Line-8
Line-9
Line-10
Line-11
Line-12
Line-13

1
290.88088
250.25662
306.20085
253.18855
277.03453
269.53039
274.80688

2
23.18417
24.87647
26.46833
23.08500
22.69333
23.55000
26.30611

3
78.35527
93.91409
96.77248
85.44022
76.77075
NA
97.99277

4
74.41062
92.31220
95.42920
83.28003
74.34624
NA
96.87625

5
4.78636
5.18156
5.00122
4.95161
4.78611
NA
5.42638

6
147.49022
207.11111
228.44444
215.91667
198.69444
188.50000
254.41667

7
118.38889
145.23529
133.77778
143.70588
134.16667
143.00000
147.77778

8
20.31309
22.60133
23.83731
21.74047
20.04489
NA
22.41225

9
16.11765
15.88889
14.00000
15.44444
14.88889
14.94444
16.77778

10
325.08333
327.14545
363.70417
405.72083
338.24167
345.32083
369.68750

11
1.15933
1.29167
1.37067
1.29550
1.19217
1.13100
1.52650

12
1.79967
1.59533
1.73867
1.68133
1.56467
1.42133
1.89067

13
1.00000
1.05556
1.00000
1.00000
1.00000
1.00000
1.00000

14
0.93033
0.98246
0.98585
0.97443
0.96633
NA
0.98859

15
3.24588
3.18218
3.29132
3.26875
3.21585
3.15483
3.38357

16
0.66499
0.64595
0.70466
0.67820
0.66995
0.65171
0.72309

17
1.14189
1.11850
1.15068
1.16333
1.12373
1.08997
1.20621

18
0.73957
0.72970
0.77352
0.73881
0.75647
0.75748
0.75969

19
0.81991
0.92149
1.01686
0.94248
0.85244
0.81325
1.14172

20
0.93960
1.04993
1.15477
1.07557
0.97430
0.92433
1.28740

21
0.13665
0.15358
0.16948
0.15708
0.14207
0.13554
0.19029

22
212.41250
181.43182
199.22083
206.90833
168.54167
199.42083
200.12083

23
116.75000
106.94500
85.97333
102.70750
105.73250
102.11750
143.06250

24
7123.47500
6075.20556
6597.66625
6030.40000
6307.05667
6617.64889
6848.03429

25
3984.75000
3696.75000
3926.66667
3127.66667
3942.75000
3955.00000
4854.00000

26
33.18518
33.65926
33.78147
32.63890
33.95001
33.27778
33.90186

27
27.60583
25.26364
26.17833
34.31182
25.49917
23.06292
25.58750

28
20.60278
16.34667
18.90059
27.30389
22.34833
19.25500
22.81556

29
60.35750
63.06667
55.88500
82.12500
60.02250
58.69500
116.11500

30
18.08333
20.18182
19.80833
22.89167
19.80839
19.53333
21.40000

31
17.07222
20.69444
18.47778
23.30556
19.38889
19.65556
19.96667

32
15.00000
18.67500
20.50000
22.56667
19.82500
14.50000
20.33333

33
18.10500
17.09364
16.86750
17.49167
16.61583
17.09917
17.37583

34
17.96167
18.42111
17.43444
18.07111
17.68111
17.61278
18.93333

35
23.47333
20.97250
21.45750
21.40500
22.11500
23.24750
24.31000

36
14.27778
14.72222
15.44444
14.33333
14.44444
14.88889
14.38889

37
244.25000
273.55556
273.22222
295.33333
259.25000
257.88889
277.19444

38
4.82134
6.01201
5.98694
6.66171
5.98578
5.61817
6.53028

39
55.37593
56.75925
55.81176
58.54074
51.67778
55.15741
54.15556

40
507.78333
549.33636
509.73750
662.12917
527.43333
474.67833
544.03182

41
660.69500
724.57500
618.46000
837.56250
612.80500
727.99750
950.28750

42
2.23267
2.72650
2.33133
2.39550
2.19950
2.08367
2.83983

43
15.84909
14.39455
17.84833
20.42333
13.92917
13.05250
16.45083

44
9.72333
6.98056
9.39556
13.58278
9.20222
7.68667
10.17059

45
17.06667
17.51818
18.15000
18.60833
17.69167
18.15000
18.64167

46
16.41667
18.33889
16.62778
19.38333
16.71111
16.26667
15.92222

47
15.04250
13.62818
14.73333
14.60750
13.16917
12.77000
14.15333

48
13.09556
13.48176
13.41722
13.27111
13.13556
12.53000
13.79222

49
1.07333
1.43800
0.96067
1.10000
1.00733
0.95267
1.31333

50
2.21967
2.89733
2.22400
2.82667
2.29467
2.15133
2.90000

Table 72. Provided are the values of each of the parameters (as described above) measured in Maize accessions (line) under normal growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 73

Measured parameters in Maize Hybrid under defoliation

Ecotype/Treatment
Line-1
Line-2
Line-3
Line-4
Line-5
Line-6

1
280.0253
251.8588
294.2919
295.3616
288.3955
308.2517

2
19.0275
22.1153
16.3058
21.5365
19.8383
18.2094

3
53.6001
45.5028
38.3068
58.4717
53.8938
63.5379

4
51.4969
42.9524
34.5905
55.6745
51.3574
61.4371

5
4.1811
4.2072
3.9194
4.7733
4.5058
4.6122

6
89.2020
100.7500
73.3889
129.8389
129.7778
115.0556

7
119.4444
131.5556
145.5278
156.0556
145.2778
129.5294

8
16.3379
13.6259
12.8890
15.9372
15.3364
17.5304

9
12.7059
14.3571
13.0000
14.1176
13.4706
13.0714

10
0.7470
0.5832
0.4403
0.7415
0.7787
0.5762

11
0.9727
0.8333
0.6287
0.9793
1.0100
0.8033

12
1.0000
0.9444
1.0000
0.9444
1.0000
0.9412

13
0.9539
0.9147
0.8725
0.9495
0.9477
0.9613

14
3.1085
3.1441
3.1793
3.2073
3.1963
3.2300

15
0.6485
0.6321
0.6692
0.6752
0.6767
0.6833

16
1.0517
1.0795
1.0787
1.1102
1.0869
1.0944

17
0.5227
0.4001
0.2890
0.5168
0.5465
0.3983

18
0.6042
0.4557
0.3308
0.5883
0.6236
0.4581

19
0.0871
0.0687
0.0482
0.0902
0.0911
0.0798

20
112.2700
94.9850
125.1375
144.4825
112.5025
116.1575

21
3914.0000
3480.0000
4276.5000
4985.5000
4643.5000
4223.0000

22
23.0211
26.5017
26.9750
15.2372
18.1917
37.2128

23
64.1600
53.8125
56.4125
80.9500
71.2700
66.6850

24
16.2944
21.4389
20.8500
22.5778
22.9389
21.6222

25
15.1500
18.5000
16.6667
18.0667
18.0000
19.8333

26
19.5389
16.8994
15.7933
17.0128
17.1206
18.1744

27
24.3000
20.5650
21.0575
24.8700
20.8525
20.4550

28
15.1667
14.3889
15.0000
15.1111
14.5000
14.2222

29
251.4167
248.6389
268.0556
285.1111
278.8333
261.8824

30
6.3849
6.3189
6.3147
6.9319
6.8319
7.1421

31
61.2130
57.3630
58.0222
62.3593
60.7204
62.2241

32
713.5400
538.0425
705.5250
803.3250
703.3575
664.2250

33
1.5393
1.3652
1.4403
1.5315
1.5707
1.5738

34
8.6794
11.0750
14.0950
4.8928
6.0350
13.9544

35
16.2389
18.8333
17.7444
19.6389
20.7389
20.1389

36
14.2722
12.8217
12.6861
11.0906
12.0039
13.0250

37
0.7923
0.7820
1.0000
0.7900
0.7920
0.9977

38
2.5113
1.9553
2.7967
2.1073
2.2047
2.7853

Table 73. Provided are the values of each of the parameters (as described above) measured in Maize accessions (line) under defoliation growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 74

Measured parameters in Maize Hybrid under defoliation, additional maize lines

Ecotype/

Treatment
Line-7
Line-8
Line-9
Line-10
Line-11
Line-12
Line-13

1
230.1190
271.2500
259.4268
243.9760
262.4103
248.6401
244.1569

2
19.7681
22.4406
20.2827
19.6433
22.3150
23.3117
27.7825

3
39.8275
47.3287
65.8961
43.8344
43.2839
52.2972
58.3135

4
36.3128
43.3387
64.8033
39.5577
40.4291
49.2795
55.6853

5
4.0992
4.2021
4.6641
4.0562
4.0122
4.4075
4.9751

6
85.0444
33.0998
161.7611
89.3611
87.6786
88.1833
124.5833

7
123.3750
135.0000
136.5000
136.3889
130.3235
139.7059
143.4444

8
13.2138
14.8181
17.6020
13.7844
13.7459
15.5304
14.8785

9
14.0625
13.7500
13.9375
12.7857
13.0000
14.2857
15.8333

10
0.4537
0.6302
0.8027
0.5362
0.5518
0.5122
0.7475

11
0.6480
0.8187
1.1480
0.8773
0.7913
0.6927
0.9913

12
0.8889
1.0000
0.8824
1.0000
1.0556
0.9444
1.0000

13
0.9051
0.9051
0.9827
0.8896
0.9177
0.9401
0.9502

14
3.1299
3.0157
3.1165
3.0856
3.0298
2.9757
3.1526

15
0.6308
0.6098
0.6230
0.6191
0.6004
0.5830
0.6306

16
1.0659
1.0242
1.0836
1.0543
1.0245
0.9952
1.0946

17
0.3015
0.4386
0.6670
0.3594
0.3771
0.3444
0.5309

18
0.3451
0.5050
0.7674
0.4115
0.4346
0.3942
0.6087

19
0.0564
0.0731
0.1239
0.0599
0.0628
0.0589
0.0885

20
113.7825
93.7375
89.8575
86.9825
117.2700
150.6825
161.6450

21
3436.000
4593.0000
4315.5000
4020.5000
4154.0000
4851.5000
3750.0000

22
27.8847
17.3294
20.5100
25.3622
28.4144
23.1644
38.7961

23
64.1875
76.2333
57.8500
69.9750
67.3000
72.9000
83.5833

24
18.7588
20.8833
17.8278
20.7000
20.4278
20.1111
24.1278

25
16.1000
14.8333
17.5000
23.6667
19.0000
16.4500
20.6000

26
18.2147
17.2333
17.8822
17.1239
17.5256
18.6272
19.8653

27
20.9550
22.4700
21.2300
19.8475
21.2925
23.5800
21.3675

28
14.3889
14.6667
15.6111
14.3889
14.0556
14.6111
14.0000

29
254.6389
261.9444
268.8778
272.7059
262.5000
266.3333
279.1389

30
6.4818
6.2823
7.0439
7.2035
7.3391
6.9388
7.2743

31
59.6537
59.9852
56.7611
65.6963
57.9407
60.3056
57.7148

32
673.2375
738.3675
692.2250
619.7875
729.2250
794.6375
847.5167

33
1.3370
1.4742
1.6627
1.4765
1.3135
1.4762
1.7148

34
10.9339
6.4767
9.0072
10.6856
10.3789
8.4900
12.2878

35
17.1833
19.1167
16.7389
15.9556
17.3111
18.1944
17.7722

36
14.2539
12.7689
13.5206
13.0783
13.4317
13.2117
14.7244

37
0.8833
0.8440
0.8600
0.9403
0.7617
0.9640
0.9673

38
2.5413
2.4753
2.3500
2.5947
2.4060
2.6993
2.7207

Table 74. Provided are the values of each of the parameters (as described above) measured in Maize accessions (line) under defoliation growth conditions. Growth conditions are specified in the experimental procedure section.

Tables 75 and 76 hereinbelow provide the correlations (R) between the expression levels yield improving genes and their homologs in various tissues [Expression (Exp) sets] and the phenotypic performance [yield, biomass, growth rate and/or vigor components (Correlation vector (Cor))] under normal and defoliation conditions across maize varieties. P=p value.

TABLE 75

Correlation between the expression level of selected genes of some embodiments of

the invention in various tissues and the phenotypic performance under normal

conditions across maize varieties

Corr.

Corr.

Gene

Exp.
Set
Gene

Exp.
Set

Name
R
P value
set
ID
Name
R
P value
set
ID

LAB673
0.761
4.04E−03
3
46
LAB673
0.700
1.12E−02
3
18

LAB673
0.722
1.84E−02
4
5
LAB676
0.786
4.12E−03
4
9

LAB676
0.786
4.09E−03
4
45
LAB676
0.760
1.08E−02
4
5

LAB676
0.728
1.11E−02
2
7
LAB677
0.710
1.44E−02
4
45

LAB677
0.703
1.58E−02
4
10
LAB677
0.767
9.68E−03
2
5

LAB678
0.759
2.62E−03
1
26
LAB678
0.829
8.64E−04
3
13

LAB678
0.978
2.17E−07
4
13
LAB678
0.867
5.55E−04
2
20

LAB678
0.905
1.25E−04
2
11
LAB678
0.712
1.40E−02
2
2

LAB678
0.773
5.26E−03
2
42
LAB678
0.849
1.88E−03
2
4

LAB678
0.776
8.32E−03
2
8
LAB678
0.841
2.28E−03
2
3

LAB678
0.707
2.23E−02
2
5
LAB678
0.866
5.74E−04
2
21

LAB678
0.866
5.74E−04
2
19
LAB679
0.741
5.85E−03
3
27

LAB679
0.780
2.78E−03
3
43
LAB679
0.818
1.15E−03
3
28

LAB679
0.766
3.70E−03
3
10
LAB679
0.749
5.06E−03
3
34

LAB679
0.763
6.29E−03
4
41
LAB679
0.731
1.06E−02
4
10

LAB680
0.713
6.22E−03
1
48
LAB680
0.766
2.28E−03
1
47

LAB680
0.725
5.05E−03
1
27
LAB680
0.723
7.83E−03
3
34

LAB680
0.820
1.99E−03
2
41
LAB681
0.731
1.06E−02
4
31

LAB681
0.713
1.38E−02
2
46
LAB682
0.707
1.50E−02
4
45

LAB683
0.736
9.84E−03
4
20
LAB683
0.785
4.17E−03
4
11

LAB683
0.781
4.53E−03
4
29
LAB683
0.715
1.34E−02
4
17

LAB683
0.765
9.91E−03
4
5
LAB683
0.733
1.03E−02
4
21

LAB683
0.733
1.03E−02
4
19
LAB683
0.724
1.17E−02
2
37

LAB684
0.749
7.97E−03
4
41
LAB684
0.719
1.27E−02
4
45

LAB684
0.920
6.20E−05
4
29
LAB684
0.815
2.23E−03
4
17

LAB684
0.754
7.33E−03
4
15
LAB684
0.782
4.45E−03
4
30

LAB684
0.832
1.49E−03
2
9
LAB685
0.758
4.28E−03
3
18

LAB687
0.739
3.88E−03
1
27
LAB687
0.784
1.52E−03
1
40

LAB687
0.744
3.52E−03
1
15
LAB687
0.759
6.75E−03
4
9

LAB688
0.728
7.29E−03
3
45
LAB688
0.713
9.30E−03
3
28

LAB688
0.742
5.76E−03
3
10
LAB688
0.715
1.34E−02
4
10

LAB688
0.721
1.22E−02
4
18
LAB689
0.780
4.66E−03
4
9

LAB692
0.743
5.65E−03
3
13
LAB692
0.746
8.45E−03
4
30

LAB692
0.701
1.62E−02
2
38
LAB692
0.803
5.16E−03
2
8

LAB693
0.729
4.70E−03
1
33
LAB693
0.724
5.18E−03
1
27

LAB693
0.731
4.54E−03
1
10
LAB693
0.719
5.65E−03
1
34

LAB693
0.711
9.55E−03
3
16
LAB693
0.707
1.02E−02
3
15

LAB693
0.739
9.35E−03
2
24
LAB693
0.822
1.91E−03
2
1

LAB694
0.708
2.19E−02
2
8
LAB696
0.860
3.35E−04
3
20

LAB696
0.901
6.41E−05
3
11
LAB696
0.723
7.90E−03
3
38

LAB696
0.768
3.51E−03
3
37
LAB696
0.896
8.03E−05
3
42

LAB696
0.702
1.10E−02
3
6
LAB696
0.741
5.80E−03
3
41

LAB696
0.804
2.88E−03
3
4
LAB696
0.776
3.02E−03
3
29

LAB696
0.731
6.92E−03
3
17
LAB696
0.808
2.65E−03
3
3

LAB696
0.775
5.04E−03
3
5
LAB696
0.708
1.00E−02
3
49

LAB696
0.759
4.16E−03
3
50
LAB696
0.852
4.38E−04
3
21

LAB696
0.852
4.38E−04
3
19
LAB696
0.733
1.02E−02
2
6

LAB696
0.707
1.49E−02
2
41
LAB696
0.807
2.70E−03
2
29

LAB697
0.704
7.24E−03
1
13
LAB697
0.718
8.53E−03
3
42

LAB697
0.719
8.42E−03
3
34
LAB697
0.731
1.06E−02
4
38

LAB697
0.852
8.72E−04
4
41
LAB697
0.849
9.52E−04
4
29

LAB697
0.799
3.20E−03
4
30
LAB697
0.702
1.61E−02
4
10

LAB697
0.761
6.49E−03
2
10
LAB698
0.731
4.56E−03
1
22

LAB698
0.725
1.15E−02
4
40
LAB698
0.800
3.13E−03
4
46

LAB700
0.752
4.75E−03
3
25
LAB700
0.749
5.06E−03
3
42

LAB700
0.748
5.13E−03
3
29
LAB700
0.840
6.35E−04
3
23

LAB701
0.718
5.74E−03
1
48
LAB701
0.737
4.06E−03
1
33

LAB701
0.775
1.84E−03
1
34
LAB702
0.817
2.15E−03
4
13

LAB703
0.741
3.72E−03
1
33
LAB704
0.720
8.26E−03
3
25

LAB704
0.758
4.29E−03
3
23
LAB704
0.705
1.54E−02
4
40

LAB704
0.718
1.29E−02
4
28
LAB704
0.712
1.39E−02
4
30

LAB704
0.838
1.29E−03
4
10
LAB705
0.711
9.50E−03
3
1

LAB706
0.745
8.50E−03
4
9
LAB706
0.802
2.99E−03
2
24

LAB707
0.856
7.72E−04
2
25
LAB707
0.727
1.13E−02
2
1

LAB708
0.720
8.26E−03
3
25
LAB708
0.704
1.06E−02
3
16

LAB708
0.725
7.61E−03
3
23
LAB708
0.760
6.66E−03
4
38

LAB708
0.737
9.71E−03
4
31
LAB708
0.827
1.71E−03
4
37

LAB708
0.734
1.00E−02
4
41
LAB708
0.763
6.33E−03
4
30

LAB708
0.754
7.35E−03
2
35
LAB709
0.707
1.01E−02
3
10

LAB709
0.728
7.25E−03
3
44
LAB709
0.731
1.06E−02
4
27

LAB709
0.745
8.57E−03
4
40
LAB709
0.717
1.30E−02
4
43

LAB709
0.794
3.51E−03
4
28
LAB709
0.835
1.37E−03
4
44

LAB710
0.774
5.19E−03
4
47
LAB710
0.715
1.34E−02
4
12

LAB710
0.704
1.56E−02
4
39
LAB710
0.862
6.39E−04
2
24

LAB712
0.701
1.11E−02
3
37
LAB712
0.716
8.87E−03
3
50

LAB712
0.815
2.22E−03
4
40
LAB712
0.760
6.68E−03
2
18

LAB713
0.762
2.48E−03
1
10
LAB713
0.735
6.51E−03
3
25

LAB713
0.735
6.52E−03
3
29
LAB713
0.736
6.36E−03
3
15

LAB713
0.757
4.33E−03
3
23
LAB713
0.713
1.38E−02
4
20

LAB713
0.759
6.74E−03
4
29
LAB713
0.724
1.17E−02
4
17

LAB713
0.707
2.23E−02
4
5
LAB713
0.726
1.15E−02
4
21

LAB713
0.726
1.15E−02
4
19
LAB714
0.808
2.63E−03
4
46

Table 75. Correlations (R) between the genes expression levels in various tissues and the phenotypic performance.

“Corr. ID”—correlation set ID according to the correlated parametersTable above.

“Exp. Set”—Expression set.

“R” = Pearson correlation coefficient;

“P” = p value.

TABLE 76

Correlation between the expression level of selected genes of some embodiments of the

invention in various tissues and the phenotypic performance under defoliation conditions

across maize varieties

Corr.

Corr.

Gene

Exp.
Set
Gene

Exp.
Set

Name
R
P value
set
ID
Name
R
P value
set
ID

LAB673
0.782
2.63E−03
1
31
LAB673
0.774
3.15E−03
3
1

LAB673
0.718
8.57E−03
2
16
LAB673
0.820
1.09E−03
2
14

LAB673
0.847
5.02E−04
2
15
LAB676
0.807
1.51E−03
1
35

LAB676
0.720
8.30E−03
1
14
LAB676
0.701
1.11E−02
1
15

LAB676
0.746
5.37E−03
3
35
LAB676
0.754
4.61E−03
3
24

LAB676
0.774
3.13E−03
2
25
LAB677
0.708
1.48E−02
4
22

LAB678
0.725
7.67E−03
3
24
LAB678
0.792
2.11E−03
3
12

LAB679
0.762
3.97E−03
1
38
LAB679
0.855
7.89E−04
4
38

LAB679
0.858
7.25E−04
4
37
LAB680
0.723
7.87E−03
1
36

LAB680
0.750
4.99E−03
2
38
LAB680
0.738
6.13E−03
2
37

LAB681
0.816
1.19E−03
2
35
LAB681
0.753
7.47E−03
4
1

LAB684
0.825
9.66E−04
1
20
LAB684
0.719
8.45E−03
1
32

LAB685
0.772
3.23E−03
1
35
LAB688
0.876
1.84E−04
1
30

LAB688
0.717
8.71E−03
1
25
LAB688
0.712
9.40E−03
3
30

LAB688
0.720
8.34E−03
3
25
LAB688
0.815
2.24E−03
4
30

LAB688
0.825
1.79E−03
4
25
LAB689
0.758
6.84E−03
4
26

LAB690
0.710
9.61E−03
3
30
LAB690
0.889
1.08E−04
3
25

LAB692
0.770
3.41E−03
2
30
LAB693
0.850
4.62E−04
2
31

LAB693
0.778
4.78E−03
4
25
LAB697
0.810
1.39E−03
1
15

LAB697
0.809
1.44E−03
1
1
LAB697
0.706
1.03E−02
1
10

LAB697
0.720
8.22E−03
3
24
LAB700
0.737
6.22E−03
1
1

LAB700
0.766
3.67E−03
3
27
LAB700
0.816
1.20E−03
2
2

LAB700
0.732
6.78E−03
2
9
LAB701
0.742
5.69E−03
3
36

LAB701
0.771
3.30E−03
3
26
LAB701
0.751
7.74E−03
4
36

LAB701
0.891
2.28E−04
4
26
LAB701
0.795
3.45E−03
4
38

LAB701
0.752
7.59E−03
4
37
LAB702
0.724
7.79E−03
1
14

LAB702
0.768
3.54E−03
1
15
LAB702
0.717
8.72E−03
2
8

LAB703
0.755
4.51E−03
1
28
LAB703
0.756
4.46E−03
1
19

LAB703
0.772
3.28E−03
1
8
LAB703
0.769
3.45E−03
3
7

LAB703
0.765
6.13E−03
4
1
LAB706
0.740
9.21E−03
4
36

LAB706
0.713
1.39E−02
4
26
LAB708
0.702
1.60E−02
4
27

LAB710
0.751
4.88E−03
3
26
LAB710
0.738
6.13E−03
3
38

LAB712
0.728
7.29E−03
1
30
LAB712
0.799
1.83E−03
2
12

LAB712
0.787
4.06E−03
4
1
LAB713
0.770
3.41E−03
1
33

LAB713
0.806
1.54E−03
1
37
LAB713
0.745
5.46E−03
2
16

LAB713
0.826
9.24E−04
2
6
LAB713
0.723
7.88E−03
2
19

LAB713
0.792
3.65E−03
4
5
LAB713
0.740
9.16E−03
4
4

LAB713
0.711
1.42E−02
4
16
LAB713
0.707
1.50E−02
4
33

LAB713
0.782
4.44E−03
4
11
LAB713
0.725
1.15E−02
4
9

LAB713
0.848
9.74E−04
4
19
LAB713
0.833
1.44E−03
4
18

LAB713
0.737
9.63E−03
4
3
LAB713
0.796
3.39E−03
4
10

LAB713
0.839
1.25E−03
4
17
LAB714
0.730
1.07E−02
4
27

Table 76. Correlations (R) between the genes expression levels in various tissues and the phenotypic performance.

“Corr. ID”—correlation set ID according to the correlated parameters Table above.

“Exp. Set”—Expression set.

“R” = Pearson correlation coefficient;

“P” = p value.

Example 10
Production of Foxtail Millet Trans Grip Tom and High Throughput Correlation Analysis Using 60K Foxtail Millet Oligonucleotide Micro-Array

In order to produce a high throughput correlation analysis comparing between plant phenotype and gene expression level, the present inventors utilized a foxtail millet oligonucleotide micro-array, produced by Agilent Technologies [Hypertext Transfer Protocol://World Wide Web (dot) chem. (dot) agilent (dot) com/Scripts/PDS (dot) asp?1Page=50879]. The array oligonucleotide represents about 60K foxtail millet genes and transcripts. In order to define correlations between the levels of RNA expression and yield or vigor related parameters, various plant characteristics of 14 different foxtail millet accessions were analyzed. Among them, 11 accessions encompassing the observed variance were selected for RNA expression analysis. The correlation between the RNA levels and the characterized parameters was analyzed using Pearson correlation test [Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot) com/hyperstat/A34739 (dot) html].

Experimental Procedures

Fourteen foxtail millet varieties were grown in 5 repetitive plots, in field. Briefly, the growing protocol was as follows:

1. Regular growth conditions: foxtail millet plants were grown in the field using commercial fertilization and irrigation protocols, which include 283 m³water per dunam (100 square meters) per entire growth period and fertilization of 16 units of URAN® 32% (Nitrogen Fertilizer Solution; PCS Sales, Northbrook, Ill., USA) (normal growth conditions).

2. Drought conditions: foxtail millet seeds were sown in soil and grown under normal condition until the heading stage (22 days from sowing), and then drought treatment was imposed by irrigating plants with 50% water relative to the normal treatment (171 m³water per dunam per entire growth period).

Analyzed Foxtail millet tissues—All 15 (Above you indicated there were only 14 foxtail millet accessions) foxtail millet lines were sample per each treatment. Three tissues [leaf, flower, and stem] at 2 different developmental stages [flowering, grain filling], representing different plant characteristics were sampled and RNA was extracted as described above. Each micro-array expression information tissue type has received a Set ID as summarized in Tables 77-78 below.

TABLE 77

Foxtail millet transcriptom expression

sets under drought conditions

Expression Set
Set ID

flower: flowering stage, drought
1

leaf: flowering stage, drought
2

stem: flowering stage, drought
3

grain: grain filling stage, drought
4

leaf: grain filling stage, drought
5

stem: grain filling stage, drought
6

Table 77. Provided are the barley transcriptome expression sets under drought conditions.

TABLE 78

Foxtail millet transcriptom expression sets under normal conditions

Expression Set
Set ID

flower: flowering stage, normal
1

leaf: flowering stage, normal
2

grain: grain filling stage, normal
4

leaf: grain filling stage, normal
5

stem: grain filling stage, normal
6

Table 78. Provided are the barley transcriptome expression sets under normal conditions.

Foxtail millet yield components and vigor related parameters assessment—Plants were continuously phenotyped during the growth period and at harvest (Table 80-85, below). The image analysis system included a personal desktop computer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ 1.37 (Java based image processing program, which was developed at the U.S. National Institutes of Health and freely available on the internet [Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/]. Next, analyzed data was saved to text files and processed using the JMP statistical analysis software (SAS institute).

The following parameters were collected using digital imaging system:

At the end of the growing period the grains were separated from the Plant ‘Head’ and the following parameters were measured and collected:

Average Grain Area (cm²)—A sample of ˜200 grains was weighted, photographed and images were processed using the below described image processing system. The grain area was measured from those images and was divided by the number of grains.

Average Grain Length and width (cm)—A sample of ˜200 grains was weighted, photographed and images were processed using the below described image processing system. The sum of grain lengths and width (longest axis) were measured from those images and were divided by the number of grains.

At the end of the growing period 14 ‘Heads’ were photographed and images were processed using the below described image processing system.

Average Grain Perimeter (cm)—At the end of the growing period the grains were separated from the Plant ‘Head’. A sample of ˜200 grains were weighted, photographed and images were processed using the below described image processing system. The sum of grain perimeter was measured from those images and was divided by the number of grains.

Head Average Area (cm²)—The ‘Head’ area was measured from those images and was divided by the number of ‘Heads’.

Head Average Length and width (cm)—The ‘Head’ length and width (longest axis) were measured from those images and were divided by the number of ‘Heads’.

Additional parameters were collected either by sampling 5 plants per plot or by measuring the parameter across all the plants within the plot.

Head weight (Kg.) and head number (num.)—At the end of the experiment, heads were harvested from each plot and were counted and weighted.

Total Grain Yield (gr.)—At the end of the experiment (plant ‘Heads’) heads from plots were collected, the heads were threshed and grains were weighted. In addition, the average grain weight per head was calculated by dividing the total grain weight by number of total heads per plot (based on plot).

1000 Seeds weight [gr]—weight of 1000 seeds per plot

Biomass at harvest [kg]—At the end of the experiment the vegetative portion above ground (excluding roots) from plots was weighted.

Total dry mater per plot [kg]—Calculated as Vegetative portion above ground plus all the heads dry weight per plot.

Number (num) of days to anthesis—Calculated as the number of days from sowing till 50% of the plot arrives anthesis.

Data parameters collected are summarized in Table 79, herein below.

TABLE 79

Foxtail millet correlated parameters (vectors)

Correlated parameter with
Correlation ID

1000 grain weight (gr)
1

Biomass at harvest (1M) (Kg.)
2

Grain Perimeter (cm)
3

Grain area (cm²)
4

Grain length (cm)
5

Grain width (cm)
6

Grains yield per Head (plot) (gr)
7

Head Area (cm²)
8

Head Width (cm)
9

Head length (cm)
10

Heads num
11

Num days to Anthesis
12

Total Grains yield (gr)
13

Total dry matter (1M) (Kg.)
14

Total heads weight (Kg)
15

Table 79. Provided are the foxtail millet collected parameters.

Experimental Results

Fourteen different foxtail millet accessions were grown and characterized for different parameters as described above (Table 79). The average for each of the measured parameter was calculated using the JMP software and values are summarized in Tables 80-85 below. Subsequent correlation analysis between the various transcriptom sets and the average parameters (Tables 80-85) was conducted (Tables 86-88). Follow, results were integrated to the database.

TABLE 80

Measured parameters of correlation IDs in foxtail millet accessions under drought conditions

Line/

Corr. ID
1
2
3
4
5
6
7
8

Line-1
2.6392
1.5284
0.6825
0.0333
0.2416
0.1755
3.0533
35.7477

Line-2
3.3285
3.4592
0.7215
0.0373
0.2445
0.1943
8.8318
50.7137

Line-3
2.6105
2.8720
0.6888
0.0335
0.2496
0.1707
1.3364
18.3997

Line-4
2.2948
2.9348
0.6827
0.0319
0.2543
0.1597
1.0933
14.9379

Line-5
2.3036
3.0224
0.6902
0.0326
0.2568
0.1618
1.3094
17.6865

Line-6
2.6419
2.6648
0.6923
0.0334
0.2504
0.1701
0.4864
9.9107

Line-7
2.2151
2.9750
0.6481
0.0297
0.2331
0.1626
1.6279
20.9859

Line-8
1.8374
0.7652
0.5695
0.0238
0.1944
0.1561
3.7375
39.9290

Line-9
2.5396
2.6616
0.6607
0.0317
0.2230
0.1807
9.9001
42.1487

Line-10
1.6912
2.9464
0.5929
0.0252
0.2034
0.1581
4.1426
43.5237

Line-11
3.0961
3.2304
0.7204
0.0365
0.2608
0.1782
2.9746
26.9309

Line-12
2.5413
3.3032
0.6747
0.0321
0.2448
0.1665
1.3047
21.2295

Line-13
3.2382
2.6316
0.7484
0.0391
0.2700
0.1842
0.3629
7.3024

Line-14
2.2454
0.8856
0.6593
0.0301
0.2417
0.1586
1.7407
13.1262

Table 80: Correlation IDs: 1, 2, 3, 4, 5, . . . etc. refer to those described in Table 79 above [Foxtail millet correlated parameters (vectors)]. Provided are the values of each of the parameters (as described above) measured in Foxtail millet accessions (line) under drought growth conditions. Growth conditions are specified in the experimental procedure section

TABLE 81

Additional measured parameters of correlation IDs in foxtail millet

accessions under drought conditions

Line/Corr. ID
9
10
11
12
13
14
15

Line-1
1.8708
22.3630
374.4
34
1141.4938
0.5038
2.8880

Line-2
2.6767
21.8851
127
41
1116.1782
0.7328
6.0868

Line-3
1.3254
16.5045
737.8
51
988.2113
0.7984
5.3252

Line-4
1.3341
13.3077
1100.8
41
1202.7733
0.6160
5.4020

Line-5
1.5008
13.9981
1047.2
41
1360.5106
0.7079
5.5700

Line-6
1.1661
9.1123
2050
30
995.1714
0.4700
5.2800

Line-7
1.6655
15.0971
581.5
38
946.8482
0.6075
5.1205

Line-8
2.1528
21.1335
311.6
30
1159.7839
0.3491
2.2884

Line-9
2.3622
20.0249
147.2
38
1391.3882
0.4366
5.8340

Line-10
2.3216
21.7995
95.4
NA
394.5104
0.6448
4.3164

Line-11
1.5449
20.7968
414.4
44
1199.5016
0.7484
5.6392

Line-12
1.5902
15.8491
667.8
51
872.4820
0.8724
5.1316

Line-13
1.2536
6.4468
2441
31
873.9356
0.5228
5.1264

Line-14
1.7376
9.1779
687.5
27
1187.9820
0.3605
2.3065

Table 81: Correlation IDs: 1, 2, 3, 4, 5, . . . etc. refer to those described in Table 79 above [Foxtail millet correlated parameters (vectors)]. Provided are the values of each of the parameters (as described above) measured in Foxtail millet accessions (line) under drought growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 82

Measured parameters of correlation IDs in foxtail millet accessions for Maintenance of

performance under drought conditions

Line/

Corr. ID
1
2
3
4
5
6
7

Line-1
107.28492
63.80296
101.14903
103.09389
100.71910
102.26639
89.85420

Line-2
97.44009
86.66199
100.63477
101.05865
101.13165
100.03126
121.19054

Line-3
99.89264
90.61080
101.03545
102.80522
100.39213
102.38873
76.40597

Line-4
97.29088
81.97765
100.28207
100.87451
100.43193
100.42313
83.95708

Line-5
95.73134
84.03025
100.56979
101.56544
100.17700
101.33417
83.22790

Line-6
99.52308
87.17613
99.36660
99.75367
99.50116
100.02308
70.03712

Line-7
101.38380
73.57305
100.86771
101.13885
101.03305
100.21823
77.37223

Line-8
102.16287
66.77138
99.64822
99.96068
99.16887
100.78369
111.74037

Line-9
94.53807
83.21661
99.83736
98.88644
100.70881
98.15907
86.38569

Line-10
102.69124
75.47131
101.82094
102.67156
102.00421
100.61236
57.78836

Line-11
97.60676
90.15405
98.93543
97.94887
99.40096
98.50410
68.36558

Line-12
97.81459
89.80968
97.98844
96.37703
97.77776
98.54474
57.64576

Line-13
101.68636
89.51020
100.39095
101.18981
100.33465
100.85848
83.16443

Line-14
99.50250
59.88639
99.19422
99.24780
98.98318
100.25762
132.38018

Table 82: Correlation IDs: 1, 2, 3, 4, 5, . . . etc. refer to those described in Table 79 above [Foxtail millet correlated parameters (vectors)]. Provided are the values of each of the parameters (as described above) measured in Foxtail millet accessions (line) for maintenance of performance under drought (calculated as % of change under drought vs normal growth conditions). Growth conditions are specified in the experimental procedure section.

TABLE 83

Additional measured parameters of correlation IDs in foxtail millet accessions for

Maintenance of performance under drought conditions

Line/

Corr. ID
8
9
10
11
12
13
14

Line-1
94.50182
98.17799
96.68963
87.55847
78.74402
71.70254
75.80848

Line-2
87.63360
98.29102
90.24976
85.12064
104.52251
85.76779
102.30604

Line-3
93.93199
99.87804
93.97174
85.09804
64.38181
82.89037
85.90141

Line-4
87.35732
98.42025
89.95839
91.42857
76.74662
66.68110
95.83452

Line-5
89.50996
97.94159
91.00586
91.34682
75.80281
78.32485
88.82439

Line-6
105.26046
98.75548
106.44273
96.15385
67.41849
98.01877
86.91644

Line-7
91.55461
98.97568
93.88055
77.30657
59.82989
66.27755
81.03596

Line-8
97.65054
101.33701
96.59358
79.04617
88.00374
77.03001
81.18348

Line-9
93.05666
94.53334
98.09741
78.88532
65.27431
73.53882
80.43346

Line-10
88.21016
95.66287
93.49773
72.38240
42.06192
64.63512
82.30493

Line-11
97.27140
99.48243
99.65504
95.43989
63.79603
81.97152
85.75426

Line-12
87.80382
100.35077
88.13167
103.31064
61.13590
84.96299
87.70167

Line-13
102.45818
100.81763
101.47055
87.24712
71.85533
83.88960
91.15220

Line-14
89.37679
95.46426
93.80683
69.12327
91.61620
77.76100
84.42533

Table 83: Correlation IDs: 1, 2, 3, 4, 5, . . . etc. refer to those described in Table 79 above [Foxtail millet correlated parameters (vectors)]. Provided are the values of each of the parameters (as described above) measured in Foxtail millet accessions (line) for maintenance of performance under drought (calculated as % of change under drought vs normal growth conditions). Growth conditions are specified in the experimental procedure section.

TABLE 84

Measured parameters of correlation IDs in foxtail millet accessions under normal conditions

Line/

Corr. ID
1
2
3
4
5
6
7

Line-1
2.45995
2.39550
0.67477
0.03230
0.23989
0.17157
3.39810

Line-2
3.41596
3.99160
0.71695
0.03689
0.24172
0.19428
7.28754

Line-3
2.61327
3.16960
0.68170
0.03255
0.24860
0.16670
1.74902

Line-4
2.35874
3.58000
0.68083
0.03161
0.25317
0.15900
1.30220

Line-5
2.40635
3.59680
0.68626
0.03213
0.25634
0.15968
1.57325

Line-6
2.65459
3.05680
0.69667
0.03353
0.25168
0.16966
0.69451

Line-7
2.18488
4.04360
0.64249
0.02941
0.23076
0.16223
2.10395

Line-8
1.79847
1.14600
0.57148
0.02386
0.19607
0.15493
3.34479

Line-9
2.68629
3.19840
0.66174
0.03201
0.22145
0.18410
11.46040

Line-10
1.64690
3.90400
0.58226
0.02458
0.19936
0.15712
7.16855

Line-11
3.17197
3.58320
0.72818
0.03729
0.26240
0.18093
4.35102

Line-12
2.59803
3.67800
0.68858
0.03326
0.25037
0.16901
2.26328

Line-13
3.18446
2.94000
0.74550
0.03864
0.26910
0.18267
0.43640

Line-14
2.25661
1.47880
0.66464
0.03032
0.24416
0.15822
1.31493

Table 84: Correlation IDs: 1, 2, 3, 4, 5, . . . etc. refer to those described in Table 79 above [Foxtail millet correlated parameters (vectors)]. Provided are the values of each of the parameters (as described above) measured in Foxtail millet accessions (line) under normal growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 85

Additional measured parameters of correlation IDs in foxtail millet accessions under normal conditions

Line/

Corr. ID
8
9
10
11
12
13
14

Line-1
37.82752
1.90548
23.12861
4270.60000
1449.62601
0.70263
3.80960

Line-2
57.87014
2.72325
24.24950
149.20000
1067.88312
0.85440
5.94960

Line-3
19.58832
1.32700
17.56325
867.00000
1534.92310
0.96320
6.19920

Line-4
17.09980
1.35550
14.79317
1204.00000
1567.20040
0.92380
5.63680

Line-5
19.75921
1.53239
15.38157
1146.40000
1794.80240
0.90380
6.27080

Line-6
9.41542
1.18075
8.56073
2132.00000
1476.11048
0.47950
6.07480

Line-7
22.92173
1.68275
16.08119
752.20000
1582.56728
0.91660
6.31880

Line-8
40.88973
2.12436
21.87883
394.20000
1317.88024
0.45320
2.81880

Line-9
45.29355
2.49875
20.41332
186.60000
2131.60156
0.59370
7.25320

Line-10
49.34091
2.42686
23.31557
131.80000
937.92760
0.99760
5.24440

Line-11
27.68630
1.55289
20.86882
434.20000
1880.21340
0.91300
6.57600

Line-12
24.17832
1.58464
17.98348
646.40000
1427.11884
1.02680
5.85120

Line-13
7.12724
1.24343
6.35334
2797.80000
1216.24320
0.62320
5.62400

Line-14
14.68632
1.82013
9.78380
994.60000
1296.69424
0.46360
2.73200

Table 85: Correlation IDs: 1, 2, 3, 4, 5, ... etc. refer to those described in Table 79 above [Foxtail millet correlated parameters (vectors)]. Provided are the values of each of the parameters (as described above) measured in Foxtail millet accessions (line) under normal growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 86

Correlation between the expression level of selected genes of some

embodiments of the invention in various tissues and the phenotypic

performance under drought conditions across foxtail millet varieties

Gene

Exp.
Corr.

Name
R
P value
set
Set ID

LAB651
0.808
8.49E−03
3
15

LAB651
0.825
6.21E−03
3
2

LAB653
0.726
1.15E−02
2
13

LAB653
0.798
3.25E−03
2
9

LAB653
0.810
2.52E−03
2
7

LAB653
0.773
8.79E−03
1
14

LAB653
0.778
8.04E−03
1
12

LAB654
0.796
5.92E−03
1
13

LAB655
0.754
1.17E−02
1
1

LAB655
0.740
1.44E−02
1
10

LAB655
0.775
8.52E−03
1
4

LAB655
0.735
1.55E−02
1
3

LAB657
0.701
3.53E−02
3
6

LAB660
0.718
1.93E−02
1
10

LAB661
0.709
2.18E−02
1
13

LAB662
0.751
1.98E−02
3
5

LAB662
0.799
9.76E−03
3
11

LAB663
0.724
2.74E−02
3
1

LAB663
0.758
1.78E−02
3
6

LAB665
0.758
1.12E−02
1
10

LAB665
0.739
1.46E−02
1
7

LAB665
0.735
1.54E−02
1
8

LAB666
0.715
1.35E−02
2
13

LAB669
0.735
9.90E−03
2
4

LAB669
0.733
1.03E−02
2
3

LAB669
0.816
3.97E−03
1
1

LAB669
0.835
2.64E−03
1
4

LAB669
0.777
8.15E−03
1
3

LAB669
0.739
1.46E−02
1
6

LAB670
0.765
9.91E−03
1
13

LAB671
0.715
1.34E−02
2
15

LAB671
0.725
1.77E−02
5
2

LAB655
0.744
1.37E−02
5
9

LAB657
0.700
2.41E−02
6
4

LAB659
0.848
3.29E−02
4
1

LAB659
0.798
5.72E−02
4
4

LAB659
0.717
1.09E−01
4
3

LAB659
0.897
1.53E−02
4
6

LAB660
0.838
3.73E−02
4
14

LAB660
0.736
9.52E−02
4
12

LAB660
0.729
1.00E−01
4
9

LAB661
0.710
2.15E−02
5
9

LAB662
0.895
1.59E−02
4
11

LAB662
0.803
5.19E−03
5
13

LAB664
0.843
3.53E−02
4
1

LAB664
0.754
8.31E−02
4
4

LAB664
0.762
7.83E−02
4
9

LAB664
0.796
5.80E−02
4
7

LAB664
0.899
1.49E−02
4
6

LAB664
0.818
3.85E−03
5
11

LAB665
0.755
1.16E−02
5
9

LAB666
0.804
5.40E−02
4
12

LAB666
0.707
2.21E−02
6
13

LAB667
0.734
1.57E−02
5
9

LAB667
0.702
2.38E−02
5
7

LAB667
0.775
8.45E−03
6
13

LAB668
0.823
3.47E−03
5
9

LAB668
0.797
5.78E−03
5
7

LAB668
0.726
1.75E−02
5
8

LAB669
0.706
1.17E−01
4
5

LAB669
0.936
5.92E−03
4
11

LAB671
0.719
1.92E−02
5
5

Table 86. Correlations (R) between the genes expression levels in various tissues and the phenotypic performance.

″Corr. ID ″—correlation set ID according to the correlated parameters Table above.

″Exp. Set″—Expression set.

″R″ = Pearson correlation coefficient;

″P″ = p value.

TABLE 87

Correlation between the expression level of selected genes of some

embodiments of the invention in various tissues and

the phenotypic performance of maintenance of performance

under drought conditions across foxtail millet varieties

Gene

Exp.
Corr.

Name
R
P value
set
Set ID

LAB653
0.948
1.01E−04
3
13

LAB653
0.788
1.17E−02
3
7

LAB653
0.705
2.27E−02
1
11

LAB654
0.723
1.19E−02
2
7

LAB654
0.723
1.83E−02
1
13

LAB654
0.770
9.22E−03
1
7

LAB657
0.800
9.67E−03
3
7

LAB661
0.744
8.67E−03
2
13

LAB661
0.707
2.23E−02
1
13

LAB662
0.821
3.59E−03
1
10

LAB662
0.894
4.94E−04
1
8

LAB663
0.882
1.63E−03
3
13

LAB663
0.866
2.51E−03
3
7

LAB666
0.878
1.86E−03
3
11

LAB668
0.752
7.61E−03
2
5

LAB668
0.863
1.29E−03
1
3

LAB668
0.866
1.20E−03
1
4

LAB668
0.773
8.75E−03
1
5

LAB669
0.742
8.91E−03
2
13

LAB669
0.709
1.46E−02
2
7

LAB652
0.829
4.14E−02
4
1

LAB652
0.849
3.26E−02
4
6

LAB652
0.734
1.56E−02
5
6

LAB652
0.800
5.47E−03
6
13

LAB652
0.921
1.57E−04
6
7

LAB653
0.756
8.22E−02
4
9

LAB653
0.707
2.23E−02
6
13

LAB653
0.710
2.14E−02
6
7

LAB654
0.735
9.59E−02
4
1

LAB654
0.940
5.30E−03
4
3

LAB654
0.884
1.95E−02
4
5

LAB654
0.735
1.55E−02
5
7

LAB654
0.816
4.00E−03
6
13

LAB654
0.792
6.32E−03
6
7

LAB656
0.728
1.70E−02
5
13

LAB656
0.784
7.28E−03
5
15

LAB660
0.956
2.87E−03
4
3

LAB660
0.838
3.70E−02
4
4

LAB660
0.800
5.60E−02
4
7

LAB660
0.900
1.44E−02
4
5

LAB660
0.861
1.39E−03
6
3

LAB660
0.872
1.01E−03
6
4

LAB660
0.735
1.55E−02
6
5

LAB660
0.872
1.01E−03
6
6

LAB661
0.718
1.08E−01
4
1

LAB661
0.815
4.10E−03
6
13

LAB661
0.796
5.84E−03
6
7

LAB662
0.832
3.98E−02
4
10

LAB662
0.851
3.16E−02
4
11

LAB662
0.811
5.00E−02
4
8

LAB662
0.746
1.32E−02
6
6

LAB664
0.860
2.81E−02
4
13

LAB664
0.916
1.03E−02
4
7

LAB665
0.856
1.59E−03
6
15

LAB667
0.708
1.15E−01
4
1

LAB667
0.731
9.91E−02
4
3

LAB667
0.794
5.90E−02
4
4

LAB667
0.718
1.08E−01
4
6

LAB667
0.711
2.11E−02
6
3

LAB667
0.771
9.06E−03
6
13

LAB668
0.815
4.79E−02
4
4

LAB668
0.836
2.57E−03
5
13

LAB668
0.815
4.06E−03
5
7

LAB668
0.727
1.73E−02
5
5

LAB669
0.724
1.04E−01
4
10

LAB669
0.770
7.31E−02
4
9

LAB669
0.758
8.09E−02
4
6

LAB669
0.779
6.76E−02
4
8

LAB671
0.716
1.99E−02
5
2

LAB671
0.715
2.02E−02
5
9

Table 87. Correlations (R) between the genes expression levels in various tissues and the phenotypic performance.

″Corr. ID″—correlation set ID according to the correlated parameters Table above.

″Exp. Set″—Expression set.

″R″ = Pearson correlation coefficient;

″P″ = p value.

TABLE 88

Correlation between the expression level of selected genes of some

embodiments of the invention in various tissues and the phenotypic

performance under normal conditions across foxtail millet varieties

Gene

Exp.
Corr.

Name
R
P value
set
Set ID

LAB652
0.730
1.64E−02
2
13

LAB653
0.750
7.83E−03
1
5

LAB653
0.786
4.17E−03
1
4

LAB653
0.803
2.89E−03
1
3

LAB665
0.734
1.57E−02
2
13

LAB667
0.855
1.60E−03
2
13

LAB669
0.724
1.17E−02
1
14

LAB671
0.775
7.01E−02
3
7

LAB651
0.812
1.43E−02
5
13

LAB651
0.836
9.68E−03
5
10

LAB651
0.748
3.27E−02
5
8

LAB651
0.902
1.38E−02
3
15

LAB653
0.739
3.60E−02
5
13

LAB653
0.708
4.94E−02
4
9

LAB653
0.807
1.55E−02
5
7

LAB653
0.821
4.50E−02
3
15

LAB653
0.746
8.84E−02
3
13

LAB653
0.811
5.01E−02
3
7

LAB654
0.759
8.03E−02
3
1

LAB654
0.709
1.14E−01
3
10

LAB654
0.931
6.98E−03
3
9

LAB654
0.796
5.81E−02
3
7

LAB654
0.906
1.29E−02
3
6

LAB654
0.883
1.96E−02
3
8

LAB655
0.747
3.32E−02
5
11

LAB655
0.905
1.32E−02
3
10

LAB655
0.814
4.87E−02
3
9

LAB655
0.766
7.58E−02
3
7

LAB655
0.818
4.67E−02
3
6

LAB655
0.869
2.46E−02
3
8

LAB656
0.777
6.89E−02
3
2

LAB656
0.728
1.01E−01
3
7

LAB657
0.727
1.73E−02
4
4

LAB657
0.787
2.05E−02
5
1

LAB657
0.786
2.08E−02
5
4

LAB657
0.729
4.03E−02
5
3

LAB657
0.723
4.26E−02
5
6

LAB657
0.844
3.46E−02
3
11

LAB659
0.829
4.14E−02
3
1

LAB659
0.890
1.75E−02
3
10

LAB659
0.711
1.13E−01
3
4

LAB659
0.911
1.16E−02
3
9

LAB659
0.913
1.09E−02
3
6

LAB659
0.941
5.14E−03
3
8

LAB660
0.899
1.47E−02
3
15

LAB660
0.721
1.06E−01
3
2

LAB660
0.815
4.79E−02
3
9

LAB660
0.901
1.41E−02
3
7

LAB660
0.737
9.48E−02
3
6

LAB661
0.903
1.37E−02
3
9

LAB661
0.941
5.13E−03
3
7

LAB661
0.835
3.85E−02
3
6

LAB661
0.803
5.45E−02
3
8

LAB662
0.777
8.20E−03
4
7

LAB662
0.739
1.46E−02
4
6

LAB662
0.913
1.09E−02
3
1

LAB662
0.779
6.77E−02
3
10

LAB662
0.919
9.61E−03
3
4

LAB662
0.864
2.63E−02
3
3

LAB662
0.716
1.09E−01
3
6

LAB662
0.714
1.11E−01
3
8

LAB664
0.837
3.79E−02
3
1

LAB664
0.745
8.90E−02
3
9

LAB664
0.785
6.42E−02
3
6

LAB664
0.734
9.68E−02
3
8

LAB665
0.720
1.06E−01
3
1

LAB665
0.847
3.33E−02
3
9

LAB665
0.815
4.80E−02
3
7

LAB665
0.792
6.05E−02
3
6

LAB665
0.729
1.00E−01
3
8

LAB666
0.737
3.68E−02
5
13

LAB666
0.701
1.21E−01
3
9

LAB666
0.818
4.66E−02
3
7

LAB667
0.710
4.84E−02
5
13

LAB667
0.832
1.05E−02
5
11

LAB667
0.709
1.15E−01
3
1

LAB667
0.789
6.24E−02
3
9

LAB667
0.702
1.20E−01
3
7

LAB667
0.757
8.15E−02
3
6

LAB667
0.720
1.07E−01
3
8

LAB668
0.739
1.47E−02
4
9

LAB668
0.849
7.72E−03
5
11

LAB668
0.872
2.37E−02
3
10

LAB668
0.766
7.57E−02
3
9

LAB668
0.728
1.01E−01
3
6

LAB668
0.802
5.47E−02
3
8

LAB669
0.835
3.85E−02
3
1

LAB669
0.953
3.32E−03
3
13

LAB669
0.827
4.24E−02
3
9

LAB669
0.805
5.35E−02
3
6

LAB669
0.753
8.39E−02
3
8

LAB670
0.777
6.91E−02
3
10

LAB670
0.801
5.56E−02
3
7

LAB671
0.751
3.19E−02
5
13

Table 88. Correlations (R) between the genes expression levels in various tissues and the phenotypic performance.

″Corr. ID″—correlation set ID according to the correlated parameters Table above.

″Exp. Set″—Expression set.

″R″ = Pearson correlation coefficient;

″P″ = p value.

Example 11
Production of Barley Trans Grip Tom and High Throughput Correlation Analysis Using 44K Barley Oligonucleotide Micro-Array

In order to produce a high throughput correlation analysis comparing between plant phenotype and gene expression level under normal conditions, the present inventors utilized a Barley oligonucleotide micro-array, produced by Agilent Technologies [Hypertext Transfer Protocol://World Wide Web (dot) chem. (dot) agilent (dot) com/Scripts/PDS (dot) asp?1Page=50879]. The array oligonucleotide represents about 44,000 Barley genes and transcripts. In order to define correlations between the levels of RNA expression and yield or vigor related parameters, various plant characteristics of 25 different Barley accessions were analyzed. Among them, 13 accessions encompassing the observed variance were selected for RNA expression analysis. The correlation between the RNA levels and the characterized parameters was analyzed using Pearson correlation test [Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot) com/hyperstat/A34739 (dot) html].

Experimental Procedures

Analyzed Barley tissues—Five tissues at different developmental stages [meristem, flower, booting spike, stem and flag leaf], representing different plant characteristics, were sampled and RNA was extracted as described above. Each micro-array expression information tissue type has received a Set ID as summarized in Table 89 below.

TABLE 89

Barley transcriptom expression sets

Expression Set
Set ID

booting spike
1

flowering spike
2

meristem
3

Stem
4

Table 89. Provided are the Barley transcriptom expression sets.

Barley yield components and vigor related parameters assessment—25 Barley accessions in 4 repetitive blocks (named A, B, C, and D), each containing 4 plants per plot were grown at net house. Plants were phenotyped on a daily basis following the standard descriptor of barley (Table 90, below). Harvest was conducted while 50% of the spikes were dry to avoid spontaneous release of the seeds. Plants were separated to the vegetative part and spikes, of them, 5 spikes were threshed (grains were separated from the glumes) for additional grain analysis such as size measurement, grain count per spike and grain yield per spike. All material was oven dried and the seeds were threshed manually from the spikes prior to measurement of the seed characteristics (weight and size) using scanning and image analysis. The image analysis system included a personal desktop computer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ 1.37 [Java based image processing program, which was developed at the U.S. National Institutes of Health and freely available on the internet [Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/]. Next, analyzed data was saved to text files and processed using the JMP statistical analysis software (SAS institute).

TABLE 90

Barley standard descriptors

Trait
Parameter
Range
Description

Growth habit
Scoring
1-9
Prostrate (1) or Erect

(9)

Hairiness of
Scoring
P (Presence)/
Absence (1) or Presence

basal leaves

A (Absence)
(2)

Stem
Scoring
1-5
Green (1), Basal only

pigmentation

or Half or more (5)

Days to
Days

Days from sowing to

Flowering

emergence of awns

Plant height
Centimeter

Height from ground level

(cm)

to top of the longest

spike excluding awns

Spikes per plant
Number

Terminal Counting

Spike length
Centimeter

Terminal Counting 5

(cm)

spikes per plant

Grains per spike
Number

Terminal Counting 5

spikes per plant

Vegetative dry
Gram

Oven-dried for 48 hours

weight

at 70° C.

Spikes dry
Gram

Oven-dried for 48 hours

weight

at 30° C.

Table 90.

Grains per spike—At the end of the experiment (50% of the spikes were dry) all spikes from plots within blocks A-D were collected. The total number of grains from 5 spikes that were manually threshed was counted. The average grain per spike is calculated by dividing the total grain number by the number of spikes.

Grain average size (cm)—At the end of the experiment (50% of the spikes were dry) all spikes from plots within blocks A-D were collected. The total grains from 5 spikes that were manually threshed were scanned and images were analyzed using the digital imaging system. Grain scanning was done using Brother scanner (model DCP-135), at the 200 dpi resolution and analyzed with Image J software. The average grain size was calculated by dividing the total grain size by the total grain number.

Grain average weight (mgr)—At the end of the experiment (50% of the spikes were dry) all spikes from plots within blocks A-D were collected. The total grains from 5 spikes that were manually threshed were counted and weight. The average weight was calculated by dividing the total weight by the total grain number. “Mgr”=milligrams.

Grain yield per spike (gr.)—At the end of the experiment (50% of the spikes were dry) all spikes from plots within blocks A-D were collected. The total grains from 5 spikes that were manually threshed were weight. The grain yield was calculated by dividing the total weight by the spike number.

Spike length analysis—At the end of the experiment (50% of the spikes were dry) all spikes from plots within blocks A-D were collected. The five chosen spikes per plant were measured using measuring tape excluding the awns.

Spike number analysis—At the end of the experiment (50% of the spikes were dry) all spikes from plots within blocks A-D were collected. The spikes per plant were counted.

Growth habit scoring—At the growth stage 10 (booting), each of the plants was scored for its growth habit nature. The scale that was used was 1 for prostate nature till 9 for erect.

Hairiness of basal leaves—At the growth stage 5 (leaf sheath strongly erect; end of tillering), each of the plants was scored for its hairiness nature of the leaf before the last. The scale that was used was 1 for prostate nature till 9 for erect.

Plant height—At the harvest stage (50% of spikes were dry) each of the plants was measured for its height using measuring tape. Height was measured from ground level to top of the longest spike excluding awns.

Days to flowering—Each of the plants was monitored for flowering date. Days of flowering was calculated from sowing date till flowering date.

Stem pigmentation—At the growth stage 10 (booting), each of the plants was scored for its stem color. The scale that was used was 1 for green till 5 for full purple.

Vegetative dry weight and spike yield—At the end of the experiment (50% of the spikes were dry) all spikes and vegetative material from plots within blocks A-D were collected. The biomass and spikes weight of each plot was separated, measured and divided by the number of plants.

Dry weight=total weight of the vegetative portion above ground (excluding roots) after drying at 70° C. in oven for 48 hours.

Spike yield per plant=total spike weight per plant (gr.) after drying at 30° C. in oven for 48 hours.

Harvest Index (for barley)—The harvest index is calculated using Formula XI.

Harvest Index=Average spike dry weight per plant/(Average vegetative dry weight per plant+Average spike dry weight per plant) Formula XI

Data parameters collected are summarized in Table 91, herein below

TABLE 91

Barley correlated parameters (vectors)

Correlated parameter with
Correlation ID

Grain weight (milligrams)
1

Grains size (mm²)
2

Grains per spike (numbers)
3

Growth habit (scores 1-9)
4

Hairiness of basal leaves (scoring 1-2)
5

Plant height (cm)
6

Grain Yield per spike (gr./spike)
7

Spike length (cm)
8

Spikes per plant (number)
9

Stem pigmentation (scoring 1-5)
10

Vegetative dry weight (gram)
11

Days to flowering (days)
12

Table 91. Provided are the barley correlated parameters, “mm²” square millimeters; “gr.” = Grams; “cm” = centimeters;

Experimental Results

13 different Barley accessions were grown and characterized for parameters as described above. The average for each of the measured parameters was calculated using the JMP software and values are summarized in Table 92 below. Subsequent correlation analysis between the various transcriptom sets and the measured parameters was conducted (Table 93). Follow, results were integrated to the database.

TABLE 92

Measured parameters of correlation IDs in Barley accessions

Line/

Corr. ID
1
2
3
4
5
6
7
8
9
10
11
12

Line-1
35.05
0.27
20. 23
2.60
1.53
134.27
3.56
12.04
48.85
1.13
78.87
62.40

Line-2
28.06
0.23
17.98
2.00
1.33
130.50
2.54
10.93
48.27
2.50
66.14
64.08

Line-3
28.76
0.24
17.27
1.92
1.69
138.77
2.58
11.83
37.42
1.69
68.49
65.15

Line-4
17.87
0.17
17.73
3.17
1.08
114.58
1.57
9.90
61.92
1.75
53.39
58.92

Line-5
41.22
0.29
14.47
4.33
1.42
127.75
3.03
11.68
33.27
2.33
68.30
63.00

Line-6
29.73
0.28
16.78
2.69
1.69
129.38
2.52
11.53
41.69
2.31
74.17
70.54

Line-7
25.22
0.22
12.12
3.60
1.30
103.89
1.55
8.86
40.00
1.70
35.35
52.80

Line-8
34.99
0.28
14.07
3.50
1.19
121.63
2.62
11.22
40.63
2.19
58.33
60.88

Line-9
20.58
0.19
21.54
3.00
1.00
126.80
2.30
11.11
62.00
2.30
62.23
58.10

Line-10
27.50
0.22
12.10
3.67
1.17
99.83
1.68
8.58
49.33
1.83
38.32
53.00

Line-11
37.13
0.27
13.40
2.47
1.60
121.40
2.68
10.18
50.60
3.07
68.31
60.40

Line-12
29.56
0.27
15.28
3.50
1.08
118.42
2.35
10.51
43.09
1.58
56.15
64.58

Line-13
19.58
0.18
17.07
3.00
1.17
117.17
1.67
9.80
51.40
2.17
42.68
56.00

Table 92. Provided are the values of each of the parameters (as described above) measured in Barley accessions (line). Growth conditions are specified in the experimental procedure section

TABLE 93

Correlation between the expression level of selected LAB

genes of some embodiments of the invention in various tissues

and the phenotypic performance under normal

fertilization conditions across barley accessions

Gene

Exp.
Corr.

Name
R
P value
set
Set ID

LAB622
0.83
2.99E−03
2
9

LAB623
0.74
8.92E−03
3
6

LAB624
0.78
4.73E−03
3
9

LAB629
0.73
1.11E−02
3
9

LAB633
0.83
1.38E−03
1
6

LAB633
0.73
1.13E−02
1
8

LAB635
0.74
8.55E−03
1
2

LAB635
0.79
4.05E−03
1
1

LAB638
0.83
1.43E−03
3
2

LAB638
0.86
6.03E−04
3
1

LAB638
0.78
4.28E−03
3
8

LAB638
0.85
9.35E−04
3
7

Table 93. Correlations (R) between the genes expression levels in various tissues and the phenotypic performance.

″Corr. ID″—correlation set ID according to the correlated parameters Table above.

″Exp. Set″—Expression set.

″R″ = Pearson correlation coefficient;

″P″ = p value

Example 12
Production of Barley Trans Grip Tom and High Throughput Correlation Analysis Using 60K Barley Oligonucleotide Micro-Array

In order to produce a high throughput correlation analysis comparing between plant phenotype and gene expression level, the present inventors utilized a Barley oligonucleotide micro-array, produced by Agilent Technologies [Hypertext Transfer Protocol://World Wide Web (dot) chem. (dot) agilent (dot) com/Scripts/PDS (dot) asp?1Page=50879]. The array oligonucleotide represents about 60K Barley genes and transcripts. In order to define correlations between the levels of RNA expression and yield or vigor related parameters, various plant characteristics of 15 different Barley accessions were analyzed. Among them, 10 accessions encompassing the observed variance were selected for RNA expression analysis. The correlation between the RNA levels and the characterized parameters was analyzed using Pearson correlation test [Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot) com/hyperstat/A34739 (dot) html].

Experimental Procedures

Analyzed Barley tissues—six tissues stages [leaf, meristem, root tip, adventitious root, spike, stem] at different developmental stages [vegetative, reproductive], representing different plant characteristics, were sampled and RNA was extracted as described above. Each micro-array expression information tissue type has received a Set ID as summarized in Tables 94-96 below.

TABLE 94

Barley transcriptom expression sets

under drought and recovery conditions

Expression Set
Set ID

Booting spike under drought conditions
1

Leaf at reproductive stage under drought conditions
2

Leaf at vegetative stage under drought conditions
3

Meristem at vegetative stage under drought conditions
4

Root tip at vegetative stage under drought conditions
5

Root tip at vegetative stage under recovery from drought
6

conditions

Table 94. Provided are the barley transcriptome expression sets under drought and recovery conditions.

TABLE 95

Barley transcriptom expression sets under

normal and low nitrogen conditions (set 1)

Expression Set
Set ID

Adventitious roots under low nitrogen conditions
1

Adventitious roots under normal conditions
2

Leaf under low nitrogen conditions
3

Leaf under normal conditions
4

Root tip under low nitrogen conditions
5

Root tip under normal conditions
6

Table 95. Provided are the barley transcriptome expression sets under normal and low nitrogen conditions (set 1 - vegetative stage).

TABLE 96

Barley transcriptom expression sets under

normal and low nitrogen conditions (set 2)

Expression Set
Set ID

Booting spike under low nitrogen conditions
1

Booting spike under normal conditions
2

Leaf under low nitrogen conditions
3

Leaf under normal conditions
4

Stem under low nitrogen conditions
5

Stem under normal conditions
6

Table 96. Provided are the barley transcriptome expression sets under normal and low nitrogen conditions (set 2 - reproductive stage).

Barley yield components and vigor related parameters assessment—15 Barley accessions in 5 repetitive blocks, each containing 5 plants per pot were grown at net house. Three different treatments were applied: plants were regularly fertilized and watered during plant growth until harvesting (as recommended for commercial growth, normal growth conditions which included irrigation 2-3 times a week, and fertilization given in the first 1.5 months of the growth period); under low Nitrogen (80% percent less Nitrogen); or under drought stress (cycles of drought and re-irrigating were conducted throughout the whole experiment, overall 40% less water were given in the drought treatment). Plants were phenotyped on a daily basis following the parameters listed in Tables 97-100 below. Harvest was conducted while all the spikes were dry. All material was oven dried and the seeds were threshed manually from the spikes prior to measurement of the seed characteristics (weight and size) using scanning and image analysis. The image analysis system included a personal desktop computer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ 1.37 (Java based image processing program, which was developed at the U.S. National Institutes of Health and freely available on the internet [Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/]. Next, analyzed data was saved to text files and processed using the JMP statistical analysis software (SAS institute).

Grain yield (gr.)—At the end of the experiment all spikes of the pots were collected. The total grains from all spikes that were manually threshed were weighted. The grain yield was calculated by per plot or per plant.

Spike length and width analysis—At the end of the experiment the length and width of five chosen spikes per plant were measured using measuring tape excluding the awns.

Spike number analysis—The spikes per plant were counted.

Plant height—Each of the plants was measured for its height using measuring tape. Height was measured from ground level to top of the longest spike excluding awns at two time points at the Vegetative growth (30 days after sowing) and at harvest.

Spike weight—The biomass and spikes weight of each plot was separated, measured and divided by the number of plants.

Dry weight=total weight of the vegetative portion above ground (excluding roots) after drying at 70° C. in oven for 48 hours at two time points at the Vegetative growth (30 days after sowing) and at harvest.

Spikelet per spike=number of spikelets per spike was counted.

Root/Shoot Ratio—The Root/Shoot Ratio is calculated using Formula XII.

Root/Shoot Ratio=total weight of the root at harvest/total weight of the vegetative portion above ground at harvest. Formula XII

Total No. of tillers—all tillers were counted per plot at two time points at the Vegetative growth (30 days after sowing) and at harvest.

Percent of reproductive tillers—the number of reproductive tillers barring a spike at harvest was divided by the total numbers o tillers.

SPAD [SPAD unit]—Chlorophyll content was determined using a Minolta SPAD 502 chlorophyll meter and measurement was performed at time of flowering. SPAD meter readings were done on young fully developed leaf. Three measurements per leaf were taken per plot.

Root FW (gr.), root length (cm) and No. of lateral roots—3 plants per plot were selected for measurement of root weight, root length and for counting the number of lateral roots formed.

Shoot FW (fresh weight)—weight of 3 plants per plot were recorded at different time-points.

Average Grain Area (cm²)—At the end of the growing period the grains were separated from the spike. A sample of ˜200 grains was weighted, photographed and images were processed using the below described image processing system. The grain area was measured from those images and was divided by the number of grains.

Average Grain Length and width (cm)—At the end of the growing period the grains were separated from the spike. A sample of ˜200 grains was weighted, photographed and images were processed using the below described image processing system. The sum of grain lengths or width (longest axis) was measured from those images and was divided by the number of grains

Average Grain perimeter (cm)—At the end of the growing period the grains were separated from the spike. A sample of ˜200 grains was weighted, photographed and images were processed using the below described image processing system. The sum of grain perimeter was measured from those images and was divided by the number of grains.

Heading date—the day in which booting stage was observed was recorded and number of days from sowing to heading was calculated.

Relative water content—Fresh weight (FW) of three leaves from three plants each from different seed ID was immediately recorded; then leaves were soaked for 8 hours in distilled water at room temperature in the dark, and the turgid weight (TW) was recorded. Total dry weight (DW) was recorded after drying the leaves at 60° C. to a constant weight. Relative water content (RWC) is calculated according to Formula I above.

Harvest Index (for barley)—The harvest index is calculated using Formula XI above.

Relative growth rate: the relative growth rate (RGR) of Plant Height (Formula IX above), SPAD (Formula XIII below) and number of tillers (Formula XIII) are calculated as follows:

Relative growth rate of SPAD=Regression coefficient of SPAD measurements along time course. Formula XIII

Relative growth rate of Number of tillers=Regression coefficient of Number of tillers along time course (measured in units of “number of tillers/day”). Formula XIV

RATIO Drought/Normal: Represent ratio for the specified parameter of Drought condition results divided by Normal conditions results (maintenance of phenotype under drought in comparison to normal conditions).

Data parameters collected are summarized in Table 97-100, hereinbelow

TABLE 97

Barley correlated parameters (vectors)

under drought and recovery conditions

Correlated parameter with
Correlation ID

Chlorophyll levels
1

Dry weight at harvest (gr)
2

Dry weight vegetative growth
3

Fresh weight (gr)
4

Grain number (num)
5

Grain weight (gr)
6

Harvest index [yield/yield + biomass]
7

Heading date (days)
8

Height Relative growth rate (cm/day)
9

Number of tillers Relative growth rate (num/day)
10

Plant height (cm)
11

Root/shoot (ratio gr root/gr shoot)
12

Relative water content (%)
13

Root dry weight (gr)
14

Root fresh weight (gr)
15

Root length (cm)
16

SPAD Relative growth rate
17

Spike length (cm)
18

Spike number (num)
19

Spike weight per plant (gr)
20

Spike width (cm)
21

Tillers number (num)
22

lateral root number (num)
23

Table 97. Provided are the barley correlated parameters. ““DW” = dry weight;”

TABLE 98

Barley correlated parameters (vectors) for maintenance

of performance under drought conditions

Correlated parameter with
Correlation ID

Chlorophyll levels ratio
1

Dry weight at harvest ratio
2

Dry weight vegetative growth ratio
3

Fresh weight ratio
4

Grain number ratio
5

Grain weight ratio
6

Harvest index ratio
7

Heading date ratio
8

Plant height ratio
9

Root/shoot ratio
10

Relative water content ratio
11

Root dry weight ratio
12

Root fresh weight ratio
13

Root length ratio
14

Spike length ratio
15

Spike number ratio
16

Spike weight per plant ratio
17

Spike width ratio
18

Tillers number ratio
19

lateral root number ratio
20

Table 98. Provided are the barley correlated parameters. ““DW” = dry weight; “ratio”—ratio for the specified parameter of Drought condition results divided by Normal conditions results (maintenance of phenotype under drought in comparison to normal conditions.

TABLE 99

Barley correlated parameters (vectors) under

low nitrogen and normal conditions (set 1)

Correlated parameter with
Correlation ID

Lateral Roots, Normal (num)
1

Leaf Area, Normal (mm²)
2

Leaf Number, TP4, Low N (num)
3

Max Length, Normal (mm)
4

Max Width, Normal (mm)
5

Max Length (mm), TP4, Low N
6

Max Width (mm), TP4, Low N
7

No of lateral roots, Low N, TP2 (num)
8

No of tillers, Low N, TP2 (num)
9

Num Leaves, Normal (num)
10

Num Seeds, Normal (num)
11

Number of Spikes, Normal (num)
12

Num Tillers, Normal (num)
13

Plant Height, Normal (cm)
14

Plant Height (cm), Low N
15

Plant Height (cm), Low N-TP2
16

Root FW, Normal (gr)
17

Root Length, Normal (cm)
18

Root FW (gr.), Low N, TP2
19

Root length (cm), Low N-TP2
20

SPAD, Normal (SPAD unit)
21

SPAD, Low N, TP2 (SPAD unit)
22

Seed Yield, Normal (gr)
23

Seed Number (per plot) Low N (num)
24

Seed Yield (gr.), Low N
25

Seed Yield (gr.), Normal
26

Shoot FW, Normal (gr)
27

Spike Length, Normal (cm)
28

Spike Width, Normal (cm)
29

Spike weight, Normal (gr)
30

Spike Length (cm) Low N
31

Spike Width (cm) Low N
32

Spike total weight (per plot) Low N (gr)
33

Total Tillers, Normal (num)
34

Total Leaf Area (mm²) TP4, Low N
35

Total No of Spikes per plot Low N (num)
36

Total No of tillers per plot Low N (num)
37

shoot FW (gr.), Low N, TP2
38

Table 99. Provided are the barley correlated parameters. “TP” = time point; “DW” = dry weight; “FW” = fresh weight; “Low N” = Low Nitrogen; “Normal” = regular growth conditions. “Max” = maximum.

TABLE 100

Barley correlated parameters (vectors) under low nitrogen

and normal conditions (set 2)

Correlated parameter with
Correlation ID

Grain Perimeter (cm)
1

Grain area (cm²)
2

Grain length (cm)
3

Grain width (cm)
4

Grains DW/Shoots DW (ratio)
5

Grains per plot (num)
6

Grains weight per plant (gr)
7

Grains weight per plot (gr)
8

Plant Height (cm)
9

Roots DW (mg)
10

Row number (num)
11

Spikes FW (Harvest) (gr)
12

Spikes num (num)
13

Tillering (Harvest) (num)
14

Vegetative DW (Harvest) (gr)
15

percent of reproductive tillers (%)
16

shoot/root ratio (ratio)
17

Table 100. Provided are the barley correlated parameters. “TP” = time point; “DW” = dry weight; “FW” = fresh weight; “Low N” = Low Nitrogen; “Normal” = regular growth conditions. “Max” = maximum.

Experimental Results

15 different Barley accessions were grown and characterized for different parameters as described above. Tables 97-100 describes the Barley correlated parameters. The average for each of the measured parameters was calculated using the JMP software and values are summarized in Tables 101-109 below. Subsequent correlation analysis between the various transcriptom sets and the average parameters (Tables 110-113) was conducted. Follow, results were integrated to the database.

TABLE 101

Measured parameters of correlation IDs in Barley accessions under drought and

recovery conditions

Line/

Corr. ID
1
2
3
4
5
6
7
8
9
10
11
12

Line-1
41.33
6.15
0.21
1.90
170.00
5.55
0.47
75.00
0.27
0.07
46.00
0.01

Line-2
33.57
5.05
0.21
1.52
267.50
9.80
0.66
71.00
0.86
0.10
52.80
0.01

Line-3
36.57
3.20

1.17
111.00
3.55
0.53
65.00
0.73
0.06
35.00
0.01

Line-4
40.50
3.28

1.95
205.33
7.20
0.69

0.88
0.07
38.00
0.01

Line-5
45.07
4.76

1.90
153.60
5.28
0.53
66.75
0.40
0.16
45.20
0.03

Line-6
39.73
3.55
0.17
1.22
252.0
7.75
0.69
90.00
0.94
0.06
48.00
0.02

Line-7
38.33
4.52

1.75
288.40
9.92
0.69
90.00
0.70
0.10
37.67
0.01

Line-8
36.17
3.38

1.58
274.50
10.25
0.75

0.71
0.05
41.20
0.01

Line-9
42.13
5.67
0.25
1.88
348.50
8.50
0.60
90.00
0.77
0.10
40.80
0.01

Line-10
31.77
3.31

1.73
358.00
14.03
0.81

0.80
0.06
49.86
0.01

Line-11
33.47
2.65

1.00
521.39
17.52
0.87

0.92
0.06
43.00
0.02

Line-12
42.37
5.12
0.13
0.90
71.50
2.05
0.29
90.00
0.39
0.18
47.40
0.02

Line-13
42.27
6.86
0.19
0.90
160.13
5.38
0.44
81.60
0.88
0.15
64.80
0.01

Line-14
36.7
3.11
0.22
1.43
376.67
11.00
0.78
90.00
−0.13
0.02
52.60
0.01

Line-15
40.6
3.74

0.83
105.00
2.56
0.41

0.20
0.44
32.00
0.03

Table 101. Provided are the values of each of the parameters (as described above in Table 97) measured in Barley accessions (line) under drought growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 102

Additional measured parameters of correlation IDs in Barley accessions under drought and recovery conditions

Line/

Corr. ID
13
14
15
16
17
18
19
20
21
22
23

Line-1
80.60
77.52
2.07
21.67
0.09
16.70
4.20
17.72
8.64
11.68
8.33

Line-2
53.04
60.19
1.48
20.33
−0.12
16.85
4.36
24.24
9.07
9.04
8.67

Line-3
55.87
27.13
1.12
22.00
0.00
13.27
7.60
18.20
7.82
10.92
7.33

Line-4

18.62
1.87
24.00
0.01
13.55
8.44
18.00
7.32
10.16
7.67

Line-5
43.21
117.42
1.67
20.67
0.04
14.19
4.92
19.50
8.74
10.32
6.67

Line-6
69.78
70.72
1.68
18.33
−0.07
15.64
3.43
15.00
7.62
8.78
6.67

Line-7
45.49
37.34
1.62
21.00
0.01
15.66
6.90
23.40
6.98
13.00
7.67

Line-8
76.51
25.56
0.85
20.33
0.00
17.49
5.80
28.16
8.05
7.44
6.67

Line-9
87.41
66.18
1.45
21.67
−0.06
16.00
8.55
21.96
6.06
13.92
6.00

Line-10

22.13
1.38
19.67
0.04
18.31
9.67
33.03
6.73
11.00
8.67

Line-11

41.12
0.82
16.67
0.05
17.42
5.42
34.80
9.55
6.78
7.67

Line-12
58.32
116.95
0.58
17.00
0.00
14.23
3.05
11.73
7.87
8.45
6.33

Line-13
80.58
84.10
0.63
15.17
−0.07
14.81
4.07
18.78
7.81
9.15
7.00

Line-14
73.09
37.46
1.07
27.00
0.03
16.54
3.72
21.00
8.35
5.12
7.00

Line-15

98.86
0.70
15.00
−0.06
12.72
3.21
9.88
5.47
16.13
6.67

Table 102. Provided are the values of each of the parameters (as described above in Table 97) measured in Barley accessions (line) under drought growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 103

Measured parameters of correlation IDs in Barley accessions) for maintenance of

performance under drought conditions

Line/

Corr. ID
1
2
3
4
5
6
7
8
9
10

Line-1
0.98
0.61
0.93
0.60
0.12
0.08
0.54
0.00
0.51
1.55

Line-2
0.72
0.45
0.71
0.50
0.22
0.17
0.79
1.12
0.61
0.97

Line-3
1.30
0.59
0.00
0.47
0.11
0.06
0.58
1.30
0.67
1.12

Line-4
1.06
0.67
0.00
0.68
0.19
0.14
0.75
0.00
0.72
0.56

Line-5
1.03
0.41
0.00
0.46
0.17
0.15
0.70
1.00
0.61
1.72

Line-6
0.95
0.54
0.65
0.47
0.21
0.14
0.77
1.06
0.59
1.97

Line-7
0.82
0.75
0.00
0.58
0.22
0.15
0.75
1.37
0.70
0.67

Line-8
0.93
0.65
0.92
0.62
0.24
0.20
0.83
1.22
0.63
0.96

Line-9
0.93
0.77
1.01
0.74
0.25
0.14
0.67
0.00
0.66
1.14

Line-10
0.80
0.80
0.00

0.58
0.47
0.92

0.87
1.08

Line-11
0.94
0.68
0.00
0.81
0.43
0.32
0.93

0.86
1.38

Line-12
0.96
0.42
0.94
0.72
0.10
0.07
0.41
1.20
0.64
1.84

Line-13
1.01
0.65
0.00
0.37
0.10
0.07
0.50
1.00
0.79
1.31

Line-14
0.93
0.52
0.70
0.40
0.28
0.20
0.87

0.56
2.06

Line-15
1.03
0.46
0.00

0.43
0.32
0.82

0.51
1.46

Table 103. Provided are the values of each of the parameters (as described above in Table 98) measured in Barley accessions (line) for maintenance of performance under drought (calculated as % of change under drought vs normal growth conditions). Growth conditions are specified in the experimental procedure section.

TABLE 104

Additional measured parameters of correlation IDs in Barley accessions) for

maintenance of performance under drought conditions

Line/Corr. ID
11
12
13
14
15
16
17
18
19
20

Line-1
0.78
0.94
1.10
0.66
0.83
0.73
0.16
0.75
1.87
1.09

Line-2
0.58
0.44
1.00
0.74
0.82
0.96
0.23
0.77
1.57
0.74

Line-3
0.90
0.66
1.02
1.16
0.86
1.11
0.19
0.68
1.72
0.79

Line-4
0.00
0.37
1.67
0.78
0.77
1.30
0.23
0.67
1.80
0.88

Line-5
0.65
0.71
0.80
0.76
0.78
0.83
0.25
0.87
1.60
0.71

Line-6
0.56
1.06
0.81
0.76
0.94
0.62
0.18
0.66
1.61
0.65

Line-7
0.78
0.50
1.13
0.68
0.83
0.87
0.23
0.75
1.63
0.85

Line-8
0.83
0.62
0.34
0.77
0.89
1.12
0.34
0.74
1.59
0.77

Line-9
0.50
0.88
0.85
1.12
0.78
1.09
0.22
0.74
1.75
0.58

Line-10

0.87
0.58
0.56
0.94
1.09
0.68
0.86
1.33
0.96

Line-11
0.00
0.94
0.07
0.42
0.88
0.92
0.55
0.85
1.62
0.88

Line-12
0.00
0.77
1.06
0.82
0.77
0.49
0.18
0.79
1.33
0.95

Line-13
0.78
0.85
0.30
0.43
0.86
0.65
0.18
0.72
1.40
0.78

Line-14
0.55
1.06
0.44
0.71
0.97
0.99
0.27
0.72
1.22
0.66

Line-15

0.68
0.93
0.80
0.78
0.52
0.25
0.88
1.96
0.87

Table 104. Provided are the values of each of the parameters (as described above in Table 98) measured in Barley accessions (line) for maintenance of performance under drought (calculated as % of change under drought vs normal growth conditions). Growth conditions are specified in the experimental procedure section.

TABLE 105

Measured parameters of correlation IDs in Barley accessions) under low nitrogen and

normal conditions (set 1)

Corr.

ID/

Line
Line
Line
Line
Line
Line
Line

Line
Line-1
Line-2
Line-3
-4
-5
-6
-7
-8
-9
-10

1
7.00
8.67
8.33
9.67
10.70
9.67
9.67
8.67
10.00
9.67

2
294.00
199.00
273.00
276.00
313.00
309.00
259.00
291.00
299.00
296.00

3
8.00
8.00
7.50
8.50
10.00
11.50
8.60
6.33
7.50
10.00

4
502.00
348.00
499.00
594.00
535.00
551.00
479.00
399.00
384.00
470.00

5
5.77
5.45
5.80
6.03
4.63
5.33
5.83
5.43
5.75
6.03

6
102.90
107.78
111.57
142.42
152.38
149.33
124.08
95.00
124.12
135.17

7
5.25
5.17
5.12
5.30
5.20
5.33
5.32
5.10
5.15
5.10

8
5.00
6.00
4.33
6.00
6.33
6.00
6.67
4.67
5.67
7.33

9
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00

10
24.20
18.20
22.70
25.50
23.20
28.30
22.20
19.00
17.30
22.00

11
1090.00
510.00
242.00
582.00
621.00
1070.00
903.00
950.00
984.00
768.00

12
41.50
32.00
36.00
71.40
34.20
45.60
49.80
28.00
19.30
38.00

13
2.00
2.00
1.00
2.33
2.33
3.33
2.33
1.33
1.33
1.67

14
64.70
84.00
67.40
82.00
72.00
56.60
65.80
62.80
91.60
66.20

14
64.70
84.00
67.40
82.00
72.00
56.60
65.80
62.80
91.60
66.20

15
41.00
82.00
61.40
59.40
65.80
47.80
53.80
56.40
81.80
44.60

16
16.33
18.83
17.33
26.00
22.50
18.17
19.67
19.83
19.17
19.17

17
0.27
0.27
0.25
0.35
0.62
0.27
0.35
0.32
0.23
0.27

18
21.30
15.00
21.80
20.30
27.20
16.00
24.00
13.50
21.50
15.20

19
0.38
0.23
0.12
0.40
0.88
0.50
0.43
0.32
0.30
0.55

20
24.67
21.67
22.00
21.67
22.17
23.00
30.50
22.83
23.83
24.50

21
39.10
41.40
35.20
33.70
34.20
42.80
37.00
36.90
35.00
36.80

22
24.03
23.30
26.47
23.90
26.63
23.20
25.43
24.23
25.03
26.07

23
46.40
19.80
10.80
22.60
30.30
54.10
37.00
42.00
35.40
38.30

24
230.20
164.60
88.25
133.60
106.00
222.60
219.20
143.45
201.80
125.00

25
9.76
7.31
3.30
5.06
6.02
9.74
7.35
5.80
7.83
6.29

26
46.37
19.81
10.84
22.58
30.30
54.13
36.98
42.04
35.37
38.25

27
2.17
1.90
1.25
3.00
15.60
3.02
2.58
1.75
2.18
1.82

28
16.50
19.20
18.30
20.40
17.20
19.10
20.30
21.70
16.50
16.10

29
9.54
9.05
8.25
6.55
10.50
8.83
7.38
10.40
10.20
10.30

30
69.40
39.40
34.90
50.30
60.80
79.10
62.70
60.00
55.90
59.70

31
15.19
19.61
16.30
19.32
90.22
16.44
20.44
18.84
18.77
16.65

32
7.95
8.13
9.43
4.94
9.60
7.16
7.06
8.51
10.01
9.40

33
13.74
13.44
9.15
11.64
11.34
15.06
12.18
10.95
12.18
10.62

34
46.70
41.60
40.00
48.80
34.60
48.60
49.20
29.00
27.50
38.80

35
39.40
46.27
51.51
57.07
67.78
64.15
52.42
46.15
68.02
57.91

36
12.20
9.00
11.60
25.00
7.80
14.50
15.00
7.00
5.40
8.40

37
16.20
14.60
16.00
20.75
12.50
18.80
21.20
11.00
6.75
14.00

38
0.43
0.43
0.33
0.58
0.78
0.53
0.45
0.43
0.50
0.62

Table 105. Provided are the values of each of the parameters (as described above in Table 99) measured in Barley accessions (line) under low N and normal growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 106

Measured parameters of correlation IDs in Barley accessions) under normal conditions (set 2)

Line/Corr. ID
1
2
3
4
5
6
7
8
9

Line-1
2.24
0.25
0.89
0.35
0.40
683.40
6.65
33.24
76.40

Line-2
2.24
0.24
0.87
0.35
0.16
510.50
3.96
19.81
84.00

Line-3
2.18
0.24
0.86
0.35
1.01
1093.50
9.27
46.37
64.67

Line-4
2.05
0.23
0.80
0.37
0.79
767.60
7.65
38.25
66.20

Line-5
2.08
0.24
0.82
0.37
0.41
621.00
6.06
30.30
72.00

Line-6
2.03
0.25
0.78
0.41
0.99
1069.00
10.83
54.13
56.60

Line-7
2.25
0.24
0.90
0.35
0.66
987.75
7.94
39.69
68.00

Line-8
1.88
0.22
0.72
0.39
0.61
903.20
7.40
36.98
65.80

Line-9
2.09
0.23
0.82
0.36
0.28
581.80
4.52
22.58
82.00

Line-10
2.03
0.22
0.79
0.36
1.04
904.40
8.41
39.68
62.80

Line-11
2.02
0.23
0.80
0.37
0.12
242.40
2.00
10.84
67.40

Line-12
1.98
0.21
0.80
0.34
0.86
928.40
8.05
40.26
76.20

Line-13
1.69
0.18
0.65
0.35
0.58
984.20
7.07
35.37
91.60

Line-14
1.98
0.19
0.82
0.29
0.05
157.67
0.75
3.73
44.00

Line-15
1.89
0.17
0.77
0.29
0.08
263.25
1.14
5.68
52.75

Table 106. Provided are the values of each of the parameters (as described above in Table 100) measured in Barley accessions (line) under normal growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 107

Additional measured parameters of correlation IDs in Barley accessions)

under normal conditions (set 2)

Line/

Corr. ID
10
11
12
13
14
15
16
17

Line-1
118.30
6.00
69.84
38.60
44.25
89.20
82.30
1.48

Line-2
150.68
6.00
39.86
32.00
41.60
99.65
77.75
0.64

Line-3
86.28
6.00
69.40
41.50
46.67
45.79
86.69
0.84

Line-4
85.19
6.00
59.72
38.00
38.80
49.39
94.23
0.82

Line-5
120.31
6.00
60.83
34.20
34.60
74.32
89.74
1.15

Line-6
90.70
2.80
79.12
45.60
48.60
55.11
93.73
0.69

Line-7
40.58
6.00
63.50
30.00
32.40
47.29
89.49
1.26

Line-8
90.51
2.00
62.74
49.80
55.20
60.32
90.27
0.72

Line-9
92.59
2.00
50.30
71.40
50.60
88.01
91.21
1.17

Line-10
63.95
5.20
59.95
28.00
29.00
38.89
92.50
0.71

Line-11
286.63
6.00
34.92
36.00
40.00
97.71
91.73
0.38

Line-12
95.79
6.00
60.08
27.60
28.50
48.33
85.31
0.51

Line-13
34.04
6.00
55.88
23.60
27.50
62.52

2.16

Line-14
121.27
4.67
16.93
54.67
26.00
57.97

0.67

Line-15
206.75
4.00
21.70
48.00

72.78

0.39

Table 107. Provided are the values of each of the parameters (as described above in Table 100) measured in Barley accessions (line) under normal growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 108

Measured parameters of correlation IDs in Barley accessions)

under low nitrogen conditions (set 2)

Line/

Corr. ID
1
2
3
4
5
6
7
8
9

Line-1
2.28
0.25
0.90
0.35
0.39
153.20
1.34
6.68
75.20

Line-2
2.33
0.25
0.92
0.35
0.42
164.60
1.46
7.31
82.00

Line-3
2.28
0.25
0.93
0.35
1.25
230.20
1.95
9.76
41.00

Line-4
2.08
0.24
0.82
0.36
0.69
125.00
1.26
6.29
44.60

Line-5
2.13
0.25
0.86
0.37
0.43
100.00
1.13
5.67
65.80

Line-6
1.96
0.23
0.76
0.38
0.87
222.60
1.95
9.74
47.80

Line-7
2.09
0.23
0.83
0.35
0.77
159.40
1.28
6.40
60.60

Line-8
1.88
0.21
0.73
0.36
0.53
219.20
1.47
7.35
53.80

Line-9
2.19
0.23
0.86
0.35
0.34
133.60
0.98
5.06
59.40

Line-10
1.88
0.20
0.73
0.35
0.87
134.40
1.16
5.43
56.40

Line-11
2.03
0.22
0.81
0.35
0.15
88.25
0.92
4.62
61.40

Line-12
2.11
0.23
0.85
0.35
0.58
174.25
1.33
6.67
65.60

Line-13
1.77
0.19
0.68
0.36
0.76
201.80
1.57
7.83
81.80

Line-14
2.00
0.19
0.81
0.29
0.05
86.67
0.29
1.44
69.00

Line-15
1.90
0.17
0.79
0.27
0.07
61.60
0.22
1.12
57.40

Table 108. Provided are the values of each of the parameters (as described above in Table 100) measured in Barley accessions (line) under low N growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 109

Additional measured parameters of correlation IDs in Barley accessions) under low

nitrogen conditions (set 2)

Ecotype/

Treatment
10
11
12
13
14
15
16
17

Line-1
39.91
6
11.4
10.8
16
17.42
68.68
0.69

Line-2
26.24
6
13.44
9
14.6
17.76
61.85
1.08

Line-3
17.31
6
13.74
12.2
16.2
8.248
76.94
0.77

Line-4
32.91
6
10.62
8.4
14
7.275
59.63
0.38

Line-5
33.87
6
11.34
7.8
12.5
13.25
65.63
0.83

Line-6
83.84
2
15.06
14.5
18.8
11.318
79.84
0.42

Line-7
29.65
6
11.64
8.4
11.6
8.95
73.85
0.28

Line-8
37.21
2
12.18
15
21.2
14.18
71.01
0.57

Line-9
44.38
2
11.64
25
23.5
15.678
95.83
0.60

Line-10
14.46
5.2
8.76
7
11
6.418
64.87
0.55

Line-11
41.54
6
9.15
11.6
16
55.92
68.75
2.88

Line-12
23.75
6
12.42
7.6
10.75
11.54
74.24
1.36

Line-13
20.87
6
12.18
5.4
6.75
10.88
81.40
0.89

Line-14
49.69
2
5.68
16.4
35
58.92
37.14
2.49

Line-15
54.02
2
5.04
12

17.05

0.40

Table 109. Provided are the values of each of the parameters (as described above in Table 100) measured in Barley accessions (line) under low N growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 110

Correlation between the expression level of selected LAB

genes of some embodiments of the invention in various

tissues and the phenotypic performance under drought

stress conditions across Barley accessions

Gene

Exp.
Corr.

Name
R
P value
set
Set ID

LAB620
0.93
7.86E−04
3
11

LAB620
0.73
1.02E−01
5
13

LAB620
0.76
1.68E−02
4
17

LAB621
0.75
8.35E−02
1
18

LAB621
0.73
1.01E−01
1
6

LAB621
0.96
2.43E−03
1
11

LAB621
0.81
5.21E−02
1
20

LAB621
0.79
1.92E−02
3
17

LAB621
0.85
1.44E−02
2
19

LAB621
0.72
6.87E−02
2
16

LAB621
0.91
4.29E−03
2
4

LAB621
0.78
3.97E−02
2
15

LAB622
0.75
8.66E−02
1
18

LAB622
0.85
3.37E−02
1
11

LAB622
0.73
1.01E−01
1
20

LAB622
0.83
1.13E−02
3
19

LAB622
0.74
2.38E−02
6
16

LAB622
0.89
2.81E−03
5
19

LAB622
0.77
2.69E−02
5
22

LAB622
0.78
2.13E−02
5
16

LAB623
0.88
3.85E−03
3
11

LAB623
0.73
6.32E−02
2
11

LAB623
0.72
4.27E−02
5
11

LAB624
0.88
8.42E−03
2
12

LAB624
0.95
2.23E−04
5
17

LAB624
0.74
2.34E−02
4
19

LAB624
0.72
2.73E−02
4
17

LAB625
0.83
4.28E−02
1
18

LAB625
0.78
6.49E−02
1
6

LAB625
0.85
3.40E−02
1
20

LAB625
0.76
4.79E−02
2
6

LAB625
0.75
5.05E−02
2
11

LAB625
0.79
3.47E−02
2
20

LAB625
0.79
1.99E−02
5
14

LAB625
0.79
6.29E−02
5
8

LAB626
0.82
4.75E−02
1
7

LAB626
0.81
5.14E−02
1
18

LAB626
0.81
5.01E−02
1
6

LAB626
0.91
1.29E−02
1
11

LAB626
0.93
7.31E−03
1
20

LAB626
0.80
3.15E−02
2
21

LAB626
0.72
6.97E−02
2
12

LAB626
0.77
2.43E−02
5
18

LAB626
0.79
3.52E−02
4
13

LAB626
0.76
1.76E−02
4
14

LAB626
0.92
4.09E−04
4
1

LAB627
0.77
2.58E−02
3
12

LAB627
0.86
5.76E−03
3
14

LAB627
0.79
2.10E−02
5
14

LAB627
0.79
1.06E−02
4
14

LAB628
0.79
5.95E−02
1
23

LAB628
0.76
7.98E−02
1
6

LAB628
0.81
4.93E−02
1
11

LAB628
0.76
7.93E−02
1
20

LAB628
0.76
4.57E−02
2
6

LAB628
0.83
1.08E−02
5
17

LAB628
0.79
1.06E−02
4
19

LAB629
0.74
9.54E−02
1
21

LAB629
0.74
3.71E−02
3
21

LAB629
0.78
4.07E−02
6
13

LAB630
0.75
8.48E−02
1
21

LAB630
0.80
3.16E−02
2
7

LAB630
0.81
2.73E−02
2
17

LAB630
0.72
6.81E−02
2
20

LAB630
0.87
4.50E−03
5
19

LAB630
0.78
2.19E−02
5
22

LAB630
0.79
2.05E−02
5
4

LAB630
0.84
5.09E−03
4
19

LAB631
0.81
5.14E−02
1
10

LAB631
0.82
4.39E−02
1
12

LAB631
0.83
4.25E−02
1
2

LAB631
0.82
4.36E−02
1
14

LAB631
0.85
8.05E−03
3
11

LAB631
0.84
4.26E−03
6
23

LAB631
0.80
5.86E−02
5
13

LAB631
0.76
1.72E−02
4
11

LAB631
0.71
3.31E−02
4
14

LAB633
0.75
8.53E−02
1
17

LAB633
0.70
7.87E−02
3
13

LAB633
0.71
4.70E−02
3
12

LAB633
0.75
3.36E−02
3
14

LAB633
0.73
4.05E−02
3
1

LAB633
0.77
4.14E−02
3
8

LAB633
0.74
5.97E−02
6
13

LAB633
0.75
5.08E−02
2
7

LAB633
0.71
7.38E−02
2
23

LAB633
0.75
5.35E−02
2
17

LAB634
0.94
1.57E−03
3
8

LAB634
0.84
4.41E−03
6
5

LAB634
0.81
8.50E−03
6
6

LAB634
0.85
1.60E−02
6
8

LAB634
0.72
2.93E−02
6
20

LAB634
0.78
2.22E−02
5
17

LAB634
0.83
5.37E−03
4
7

LAB634
0.80
1.03E−02
4
5

LAB634
0.84
4.42E−03
4
6

LAB634
0.88
1.58E−03
4
20

LAB635
0.81
5.08E−02
1
10

LAB635
0.83
1.05E−02
3
11

LAB635
0.77
4.49E−02
6
13

LAB635
0.78
3.78E−02
2
7

LAB635
0.81
2.87E−02
2
21

LAB635
0.83
2.23E−02
2
5

LAB635
0.89
7.33E−03
2
6

LAB635
0.80
3.26E−02
2
12

LAB635
0.87
1.05E−02
2
20

LAB635
0.73
3.95E−02
5
9

LAB635
0.83
1.03E−02
5
11

LAB635
0.83
1.09E−02
5
14

LAB635
0.76
1.86E−02
4
11

LAB636
0.73
1.01E−01
1
7

LAB636
0.88
2.02E−02
1
11

LAB636
0.70
3.53E−02
6
2

LAB636
0.73
6.24E−02
2
16

LAB636
0.70
5.21E−02
5
4

LAB636
0.76
1.83E−02
4
19

LAB636
0.85
3.51E−03
4
22

LAB637
0.75
8.83E−02
1
7

LAB637
0.88
2.15E−02
1
18

LAB637
0.72
1.10E−01
1
6

LAB637
0.86
2.63E−02
1
20

LAB637
0.83
1.09E−02
3
21

LAB637
0.78
3.80E−02
6
8

LAB637
0.70
3.43E−02
4
10

LAB637
0.85
4.12E−03
4
11

LAB637
0.74
2.15E−02
4
14

LAB638
0.94
5.44E−03
1
23

LAB638
0.76
7.94E−02
1
6

LAB638
0.86
2.91E−02
1
11

LAB638
0.75
8.53E−02
1
17

LAB638
0.81
5.11E−02
1
20

LAB638
0.79
1.95E−02
3
12

LAB638
0.86
3.26E−03
6
11

LAB638
0.79
3.46E−02
2
23

LAB638
0.86
1.24E−02
2
11

LAB638
0.70
5.13E−02
5
23

LAB638
0.75
3.36E−02
5
11

LAB638
0.73
4.00E−02
5
14

LAB640
0.93
7.87E−03
1
12

LAB640
0.83
3.97E−02
1
2

LAB640
0.93
8.06E−03
1
14

LAB640
0.84
1.92E−02
3
13

LAB640
0.81
1.50E−02
3
1

LAB640
0.70
3.53E−02
6
5

LAB640
0.77
4.40E−02
2
21

LAB640
0.71
7.22E−02
2
6

LAB640
0.71
7.13E−02
2
17

LAB640
0.82
2.47E−02
2
14

LAB640
0.84
5.08E−03
4
17

LAB641
0.93
7.87E−03
1
12

LAB641
0.83
3.97E−02
1
2

LAB641
0.93
8.06E−03
1
14

LAB641
0.84
1.92E−02
3
13

LAB641
0.81
1.50E−02
3
1

LAB641
0.78
1.29E−02
6
10

LAB641
0.79
1.10E−02
6
11

LAB641
0.82
2.53E−02
2
21

LAB641
0.84
1.80E−02
2
12

LAB641
0.82
2.47E−02
2
14

LAB641
0.71
3.20E−02
4
19

LAB641
0.84
5.08E−03
4
17

LAB642
0.76
8.10E−02
1
10

LAB642
0.78
2.11E−02
3
11

LAB642
0.79
1.96E−02
3
2

LAB642
0.71
5.08E−02
3
14

LAB642
0.82
6.39E−03
6
20

LAB642
0.77
2.65E−02
5
10

LAB642
0.77
2.58E−02
5
11

LAB642
0.74
3.71E−02
5
2

LAB644
0.81
1.50E−02
3
14

LAB644
0.75
1.93E−02
6
5

LAB644
0.81
2.66E−02
2
5

LAB644
0.71
7.10E−02
2
6

LAB644
0.71
7.41E−02
2
11

LAB644
0.71
7.62E−02
2
20

LAB644
0.81
1.50E−02
5
11

LAB644
0.71
4.78E−02
5
2

LAB644
0.93
8.47E−04
5
14

LAB644
0.77
4.41E−02
4
13

LAB644
0.73
2.68E−02
4
14

LAB645
0.84
3.59E−02
1
18

LAB645
0.77
7.41E−02
1
6

LAB645
0.95
4.39E−03
1
11

LAB645
0.90
1.40E−02
1
20

LAB645
0.84
8.53E−03
3
19

LAB645
0.75
3.24E−02
3
20

LAB645
0.87
1.14E−02
2
11

LAB645
0.71
1.14E−01
5
13

LAB645
0.70
5.26E−02
5
17

LAB645
0.75
3.32E−02
5
20

LAB645
0.77
1.49E−02
4
17

LAB647
0.76
2.86E−02
3
2

LAB647
0.76
1.66E−02
6
17

LAB647
0.78
1.28E−02
6
20

LAB647
0.82
1.29E−02
5
10

Table 110. Correlations (R) between the genes expression levels in various tissues and the phenotypic performance.

″Corr. ID″ — correlation set ID according to the correlated parameters Table 97 above.

″Exp. Set″ — Expression set.

″R″ = Pearson correlation coefficient;

″P″ = p value.

TABLE 111

Correlation between the expression level of selected genes of

some embodiments of the invention in various tissues

and the phenotypic performance of maintenance of performance

under drought conditions across Barley accessions

Gene

Exp.
Corr.

Name
R
P value
set
Set ID

LAB620
0.70
3.47E−02
6
15

LAB620
0.73
3.91E−02
5
1

LAB620
0.71
4.96E−02
5
12

LAB620
0.74
2.31E−02
4
20

LAB621
0.91
1.24E−02
1
6

LAB621
0.92
9.67E−03
1
17

LAB621
0.91
1.06E−02
1
5

LAB621
0.70
1.21E−01
1
9

LAB621
0.81
5.03E−02
1
18

LAB621
0.71
1.12E−01
1
7

LAB621
0.78
1.36E−02
6
3

LAB621
0.74
3.43E−02
5
10

LAB621
0.78
2.20E−02
5
15

LAB621
0.76
2.73E−02
5
12

LAB622
0.72
1.05E−01
1
6

LAB622
0.74
9.15E−02
1
17

LAB622
0.74
9.54E−02
1
5

LAB622
0.75
8.53E−02
1
12

LAB622
0.88
1.65E−03
6
13

LAB622
0.75
3.20E−02
5
16

LAB622
0.70
3.49E−02
4
2

LAB624
0.70
5.25E−02
5
6

LAB624
0.80
1.64E−02
5
17

LAB624
0.75
3.21E−02
5
20

LAB624
0.71
4.78E−02
5
5

LAB624
0.77
2.61E−02
5
9

LAB624
0.92
4.27E−04
4
2

LAB625
0.71
1.13E−01
1
15

LAB625
0.83
4.25E−02
1
18

LAB625
0.85
7.79E−03
3
15

LAB625
0.85
1.55E−02
2
6

LAB625
0.88
9.85E−03
2
17

LAB625
0.89
7.73E−03
2
5

LAB625
0.91
4.50E−03
2
9

LAB625
0.92
3.75E−03
2
18

LAB625
0.70
7.77E−02
2
12

LAB626
0.95
3.59E−03
1
6

LAB626
0.98
6.55E−04
1
17

LAB626
0.86
2.62E−02
1
15

LAB626
0.90
1.39E−02
1
5

LAB626
0.73
9.99E−02
1
9

LAB626
0.86
2.79E−02
1
18

LAB626
0.86
2.72E−02
1
7

LAB626
0.73
9.63E−02
2
4

LAB627
0.77
4.28E−02
2
16

LAB627
0.76
2.83E−02
5
3

LAB628
0.91
1.25E−02
1
6

LAB628
0.90
1.48E−02
1
17

LAB628
0.91
1.17E−02
1
5

LAB628
0.93
7.36E−03
1
9

LAB628
0.89
1.72E−02
1
18

LAB628
0.70
1.20E−01
1
7

LAB628
0.70
7.74E−02
2
9

LAB628
0.74
5.81E−02
2
18

LAB628
0.88
3.52E−03
5
20

LAB628
0.77
2.50E−02
5
9

LAB628
0.74
2.18E−02
4
17

LAB628
0.71
3.19E−02
4
5

LAB628
0.75
2.04E−02
4
9

LAB628
0.89
1.42E−03
4
2

LAB629
0.77
4.27E−02
2
1

LAB629
0.85
7.14E−03
5
1

LAB630
0.78
6.75E−02
1
16

LAB630
0.73
1.02E−01
1
1

LAB630
0.76
2.80E−02
3
6

LAB630
0.74
3.67E−02
3
17

LAB630
0.73
3.78E−02
3
15

LAB630
0.81
2.62E−02
6
8

LAB630
0.79
3.52E−02
2
6

LAB630
0.86
1.34E−02
2
17

LAB630
0.74
5.66E−02
2
20

LAB630
0.73
6.02E−02
2
5

LAB630
0.81
2.55E−02
2
9

LAB630
0.80
2.98E−02
2
7

LAB630
0.82
1.29E−02
5
2

LAB631
0.80
5.37E−02
1
3

LAB631
0.76
1.80E−02
6
20

LAB631
0.80
5.85E−02
2
11

LAB633
0.81
5.28E−02
1
20

LAB633
0.80
3.11E−02
2
20

LAB633
0.78
3.92E−02
2
7

LAB633
0.78
2.36E−02
5
15

LAB633
0.75
3.05E−02
5
12

LAB634
0.72
4.59E−02
5
17

LAB634
0.75
3.22E−02
5
9

LAB634
0.76
1.79E−02
4
6

LAB634
0.79
1.19E−02
4
17

LAB634
0.78
1.26E−02
4
5

LAB634
0.75
3.07E−02
4
4

LAB634
0.78
1.22E−02
4
18

LAB634
0.82
6.36E−03
4
7

LAB635
0.81
2.57E−02
2
6

LAB635
0.88
9.29E−03
2
17

LAB635
0.85
1.58E−02
2
5

LAB635
0.92
3.79E−03
2
9

LAB635
0.91
4.27E−03
2
18

LAB635
0.77
4.13E−02
2
7

LAB635
0.72
6.91E−02
2
12

LAB636
0.75
8.43E−02
1
16

LAB636
0.83
4.32E−02
1
6

LAB636
0.82
4.56E−02
1
17

LAB636
0.80
5.42E−02
1
5

LAB636
0.77
7.17E−02
1
7

LAB636
0.77
7.60E−02
1
3

LAB636
0.73
6.28E−02
2
16

LAB636
0.79
3.56E−02
2
1

LAB636
0.94
1.58E−03
2
14

LAB637
0.71
1.12E−01
1
15

LAB637
0.76
8.18E−02
1
7

LAB638
0.94
5.27E−03
1
6

LAB638
0.95
3.71E−03
1
17

LAB638
0.77
7.20E−02
1
15

LAB638
0.82
4.76E−02
1
20

LAB638
0.93
6.87E−03
1
5

LAB638
0.95
3.32E−03
1
9

LAB638
0.90
1.50E−02
1
18

LAB638
0.73
9.99E−02
1
7

LAB638
0.75
3.15E−02
3
6

LAB638
0.75
3.18E−02
3
17

LAB638
0.91
1.60E−03
3
15

LAB638
0.74
3.60E−02
3
5

LAB638
0.74
2.14E−02
6
15

LAB638
0.92
3.50E−03
2
6

LAB638
0.88
9.85E−03
2
17

LAB638
0.74
5.75E−02
2
15

LAB638
0.70
7.82E−02
2
20

LAB638
0.88
8.35E−03
2
5

LAB638
0.77
4.28E−02
2
9

LAB638
0.76
4.87E−02
2
18

LAB638
0.71
7.42E−02
2
7

LAB638
0.80
1.82E−02
5
20

LAB638
0.77
2.41E−02
5
9

LAB639
0.82
4.57E−02
1
3

LAB639
0.71
4.87E−02
3
16

LAB639
0.75
2.05E−02
6
9

LAB639
0.71
5.06E−02
5
1

LAB640
0.72
1.08E−01
1
14

LAB640
0.73
6.27E−02
2
9

LAB640
0.82
1.30E−02
4
4

LAB640
0.71
3.28E−02
4
2

LAB641
0.72
1.08E−01
1
14

LAB641
0.72
1.07E−01
1
3

LAB641
0.75
3.20E−02
3
16

LAB641
0.82
1.30E−02
4
4

LAB641
0.72
3.00E−02
4
2

LAB642
0.76
1.65E−02
6
6

LAB642
0.85
3.47E−03
6
17

LAB642
0.71
3.05E−02
6
5

LAB642
0.85
3.82E−03
6
18

LAB644
0.80
5.67E−02
1
1

LAB644
0.78
3.83E−02
2
10

LAB644
0.71
7.50E−02
2
17

LAB644
0.78
3.76E−02
2
5

LAB644
0.72
1.05E−01
2
4

LAB644
0.71
7.50E−02
2
9

LAB644
0.83
1.98E−02
2
18

LAB644
0.94
1.92E−03
2
12

LAB644
0.76
1.81E−02
4
3

LAB645
0.91
1.06E−02
1
6

LAB645
0.94
6.08E−03
1
17

LAB645
0.78
6.67E−02
1
15

LAB645
0.92
8.24E−03
1
5

LAB645
0.92
9.94E−03
1
18

LAB645
0.76
8.00E−02
1
12

LAB645
0.84
8.34E−03
3
6

LAB645
0.88
3.72E−03
3
17

LAB645
0.88
4.10E−03
3
5

LAB645
0.76
2.95E−02
3
2

LAB645
0.85
1.64E−02
2
6

LAB645
0.79
3.42E−02
2
17

LAB645
0.84
1.79E−02
2
5

LAB645
0.74
8.93E−02
2
4

LAB645
0.82
1.33E−02
5
6

LAB645
0.90
2.64E−03
5
17

LAB645
0.84
8.29E−03
5
5

LAB645
0.72
6.57E−02
5
4

LAB645
0.77
2.61E−02
5
9

LAB645
0.70
5.30E−02
5
18

LAB645
0.76
2.71E−02
4
4

LAB645
0.78
1.34E−02
4
2

LAB647
0.73
2.51E−02
6
17

LAB647
0.75
3.07E−02
6
4

LAB647
0.83
6.00E−03
6
18

LAB647
0.76
1.84E−02
6
2

LAB647
0.89
7.68E−03−
2
3

Table 111. Correlations (R) between the genes expression levels in various tissues and the phenotypic performance.

″Corr. ID″ — correlation set ID according to the correlated parameters Table 98 above.

″Exp. Set″ — Expression set.

″R″ = Pearson correlation coefficient;

″P″ = p value.

TABLE 112

Correlation between the expression level of selected LAB genes

of some embodiments of the invention in various tissues and the

phenotypic performance under normal and low

nitrogen growth conditions across Barley accessions (set 1)

Gene

Exp.
Corr.

Name
R
P value
set
Set ID

LAB620
0.74
3.66E−02
4
28

LAB621
0.72
4.44E−02
6
21

LAB622
0.70
5.23E−02
6
10

LAB622
0.74
2.21E−02
1
20

LAB623
0.70
5.22E−02
6
21

LAB623
0.71
3.20E−02
1
26

LAB623
0.73
2.54E−02
1
25

LAB623
0.70
3.55E−02
2
11

LAB623
0.88
1.91E−03
3
31

LAB623
0.80
9.33E−03
3
19

LAB623
0.82
6.83E−03
3
38

LAB624
0.73
4.02E−02
4
1

LAB624
0.83
9.95E−03
4
27

LAB624
0.75
3.13E−02
4
17

LAB625
0.72
2.87E−02
1
19

LAB625
0.78
1.34E−02
1
35

LAB625
0.89
1.29E−03
1
38

LAB625
0.87
2.08E−03
1
6

LAB626
0.95
6.52E−05
3
31

LAB626
0.88
1.55E−03
3
19

LAB626
0.88
1.60E−03
3
38

LAB626
0.72
2.80E−02
3
6

LAB628
0.83
6.18E−03
1
20

LAB628
0.83
2.83E−03
5
20

LAB628
0.70
3.39E−02
2
18

LAB628
0.79
1.18E−02
2
17

LAB629
0.88
1.54E−03
2
21

LAB630
0.73
3.88E−02
4
10

LAB630
0.70
3.47E−02
2
34

LAB631
0.73
3.83E−02
6
17

LAB631
0.71
4.98E−02
4
30

LAB631
0.80
1.76E−02
4
2

LAB631
0.80
5.70E−03
5
19

LAB631
0.70
3.44E−02
2
21

LAB633
0.77
8.57E−03
5
19

LAB633
0.72
1.89E−02
5
3

LAB633
0.71
2.16E−02
5
8

LAB633
0.70
3.49E−02
2
21

LAB634
0.73
1.58E−02
5
6

LAB634
0.90
9.04E−04
2
27

LAB634
0.88
1.63E−03
2
17

LAB634
0.81
8.65E−03
3
31

LAB635
0.73
2.49E−02
1
33

LAB635
0.78
8.00E−03
5
25

LAB635
0.92
4.27E−04
2
1

LAB635
0.90
1.06E−03
3
7

LAB635
0.74
2.18E−02
3
37

LAB635
0.90
8.05E−04
3
36

LAB636
0.70
3.56E−02
1
31

LAB636
0.80
1.02E−02
1
19

LAB636
0.85
3.39E−03
1
20

LAB636
0.75
1.87E−02
1
38

LAB636
0.72
4.39E−02
4
12

LAB636
0.74
2.13E−02
3
31

LAB636
0.74
2.38E−02
3
7

LAB638
0.71
4.97E−02
6
28

LAB638
0.87
2.18E−03
1
16

LAB638
0.80
1.83E−02
4
21

LAB638
0.77
2.44E−02
4
14

LAB638
0.79
1.15E−02
3
36

LAB639
0.71
4.71E−02
4
28

LAB639
0.91
5.98E−04
2
29

LAB640
0.76
1.76E−02
1
20

LAB640
0.83
1.16E−02
4
4

LAB640
0.72
4.39E−02
4
17

LAB640
0.80
9.02E−03
2
14

LAB640
0.91
7.30E−04
3
36

LAB640
0.81
8.48E−03
3
16

LAB641
0.76
1.76E−02
1
20

LAB641
0.76
1.76E−02
1
38

LAB641
0.83
1.16E−02
4
4

LAB641
0.73
1.63E−02
5
3

LAB641
0.80
9.02E−03
2
14

LAB641
0.72
3.00E−02
3
19

LAB641
0.79
1.19E−02
3
3

LAB641
0.83
5.87E−03
3
38

LAB641
0.71
3.26E−02
3
36

LAB642
0.78
7.43E−03
5
3

LAB644
0.78
2.14E−02
6
5

LAB644
0.77
1.45E−02
1
26

LAB644
0.79
2.04E−02
4
1

LAB644
0.80
1.01E−02
3
31

LAB644
0.81
8.26E−03
3
19

LAB645
0.83
5.30E−03
1
16

LAB645
0.73
4.01E−02
4
34

LAB645
0.70
5.20E−02
4
12

LAB645
0.73
3.90E−02
4
10

LAB645
0.71
4.75E−02
4
11

LAB645
0.74
3.69E−02
4
30

LAB645
0.78
2.29E−02
4
13

LAB645
0.71
4.63E−02
4
23

LAB647
0.71
4.98E−02
4
12

LAB647
0.72
2.86E−02
3
31

LAB647
0.71
3.14E−02
3
19

Table 112. Correlations (R) between the genes expression levels in various tissues and the phenotypic performance.

″Corr. ID″ — correlation set ID according to the correlated parameters Table 99 above.

″Exp. Set″ — Expression set.

″R″ = Pearson correlation coefficient;

″P″ = p value.

TABLE 113

Correlation between the expression level of selected LAB genes of

some embodiments of the invention in various tissues and

the phenotypic performance under low nitrogen

and normal growth conditions across Barley accessions (set 2)

Gene

Exp.
Corr.

Name
R
P value
set
Set ID

LAB620
0.79
6.60E−03
4
5

LAB621
0.74
1.45E−02
4
17

LAB623
0.71
2.27E−02
2
6

LAB623
0.79
6.46E−03
2
7

LAB623
0.83
3.06E−03
2
8

LAB624
0.72
1.86E−02
2
13

LAB624
0.72
1.80E−02
3
2

LAB624
0.74
1.48E−02
4
15

LAB625
0.73
1.70E−02
3
1

LAB625
0.71
2.05E−02
3
3

LAB625
0.79
6.30E−03
5
17

LAB626
0.76
1.04E−02
2
6

LAB626
0.71
2.24E−02
2
8

LAB626
0.73
1.76E−02
5
17

LAB626
0.73
1.55E−02
1
17

LAB628
0.78
8.17E−03
2
17

LAB629
0.77
9.07E−03
5
9

LAB630
0.81
4.94E−03
3
17

LAB630
0.78
7.67E−03
5
17

LAB631
0.87
1.15E−03
6
12

LAB631
0.77
8.67E−03
5
10

LAB631
0.72
1.99E−02
4
10

LAB632
0.73
1.72E−02
3
4

LAB632
0.73
1.58E−02
3
10

LAB634
0.73
1.74E−02
5
17

LAB635
0.79
6.82E−03
3
17

LAB635
0.82
3.49E−03
3
9

LAB635
0.76
1.11E−02
4
11

LAB636
0.71
2.08E−02
2
6

LAB636
0.73
1.57E−02
2
5

LAB636
0.70
2.33E−02
2
8

LAB636
0.78
8.41E−03
4
10

LAB636
0.71
2.03E−02
1
9

LAB637
0.84
2.49E−03
2
6

LAB637
0.79
6.56E−03
2
7

LAB637
0.83
3.22E−03
2
8

LAB637
0.70
2.38E−02
2
12

LAB637
0.77
9.60E−03
4
10

LAB637
0.77
9.48E−03
1
12

LAB639
0.76
1.13E−02
2
6

LAB640
0.81
4.14E−03
2
6

LAB640
0.71
2.17E−02
2
5

LAB640
0.84
2.53E−03
2
7

LAB640
0.84
2.27E−03
2
8

LAB640
0.77
8.67E−03
4
5

LAB640
0.76
1.74E−02
4
16

LAB641
0.71
2.02E−02
5
4

LAB641
0.77
8.67E−03
4
5

LAB642
0.70
2.33E−02
2
14

LAB642
0.85
1.78E−03
5
9

LAB645
0.76
1.07E−02
3
6

LAB645
0.71
2.20E−02
1
6

LAB645
0.77
8.50E−03
1
12

LAB647
0.76
1.14E−02
5
9

LAB647
0.76
1.12E−02
1
9

Table 113. Correlations (R) between the genes expression levels in various tissues and the phenotypic performance.

″Corr. ID″ — correlation set ID according to the correlated parameters Table 100 above.

″Exp. Set″ — Expression set.

″R″ = Pearson correlation coefficient;

″P″ = p value.

Example 13
Production of Tomato Transcriptom and High Throughput Correlation Analysis Using 44K Tomato Oligonucleotide Micro-Array

In order to produce a high throughput correlation analysis between ABST and NUE related phenotypes and gene expression, the present inventors utilized a Tomato oligonucleotide micro-array, produced by Agilent Technologies [Hypertext Transfer Protocol://World Wide Web (dot) chem. (dot) agilent (dot) com/Scripts/PDS (dot) asp?1Page=50879]. The array oligonucleotide represents about 44,000 Tomato genes and transcripts. In order to define correlations between the levels of RNA expression with ABST, NUE, yield components or vigor related parameters various plant characteristics of 18 different Tomato varieties were analyzed. Among them, 10 varieties encompassing the observed variance were selected for RNA expression analysis. The correlation between the RNA levels and the characterized parameters was analyzed using Pearson correlation test [Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot) com/hyperstat/A34739 (dot) html].

I. Correlation of Tomato Varieties Across Ecotypes Grown Under Drought, Low Nitrogen and Regular Growth Conditions

Experimental Procedures:

Ten Tomato varieties were grown in 3 repetitive blocks, each containing 6 plants per plot, at net house. Briefly, the growing protocol was as follows:

1. Regular growth conditions: Tomato varieties were grown under normal conditions: 4-6 Liters/m²of water per day and fertilized with NPK (nitrogen, phosphorous and potassium at a ratio 6:6:6, respectively) as recommended in protocols for commercial tomato production.

2. Drought stress: Tomato varieties were grown under normal conditions (4-6 Liters/m²per day with fertilizers) until flowering. At this time, irrigation was reduced to 50% compared to normal conditions.

3. Low Nitrogen fertilization conditions: Tomato varieties were grown under normal conditions (4-6 Liters/m²per day and fertilized with NPK as recommended in protocols for commercial tomato production) until flowering. At this time, Nitrogen fertilization was stopped.

Plants were phenotyped on a daily basis following the standard descriptor of tomato (Table 115). Harvest was conducted while 50% of the fruits were red (mature). Plants were separated to the vegetative part and fruits, of them, 2 nodes were analyzed for additional inflorescent parameters such as size, number of flowers, and inflorescent weight. Fresh weight of all vegetative material was measured. Fruits were separated to colors (red vs. green) and in accordance with the fruit size (small, medium and large). Next, analyzed data was saved to text files and processed using the JMP statistical analysis software (SAS institute).

Analyzed tomato tissues—Two tissues at different developmental stages [flower and leaf], representing different plant characteristics, were sampled and RNA was extracted as described above. For convenience, each micro-array expression information tissue type has received a Set ID as summarized in Table 114 below.

TABLE 114

Tomato transcriptom expression sets

Expression Set
Set IDs

Leaf, normal conditions
1

Flower, normal conditions
2

Leaf, low nitrogen conditions
3

Flower, low nitrogen conditions
4

Leaf, drought conditions
5

Flower, drought conditions
6

Leaf, drought conditions
7

Flower, drought conditions
8

Leaf, low nitrogen conditions
9

Flower, low nitrogen conditions
10

Leaf, normal conditions
11

Flower, normal conditions
12

Table 114: Provided are the tomato transcriptome expression sets.

The average for each of the measured parameters was calculated using the JMP software and values are summarized in Tables 116-122 below. Subsequent correlation analysis was conducted (Table 123) with the correlation coefficient (R) and the p-values. Results were integrated to the database.

TABLE 115

Tomato correlated parameters (vectors)

Correlated parameter with
Correlation ID

100 weight green fruit (Drought) [gr]
1

100 weight green fruit (Low N) [gr]
2

100 weight green fruit (Normal) [gr]
3

100 weight red fruit (Drought) [gr]
4

100 weight red fruit (Low N) [gr]
5

100 weight red fruit (Normal) [gr]
6

Cluster (flower) Weight NUE/Normal [gr]
7

FW NUE/Normal
8

FW drought/Normal
9

FW/Plant (NUE) [gr]
10

FW/Plant (Normal) [gr]
11

FW/Plant Drought [gr]
12

Fruit Drought/NUE
13

Fruit NUE/Normal
14

Fruit Yield Drought/Normal
15

Fruit Yield/Plant (NUE) [gr]
16

Fruit Yield/Plant Drought [gr]
17

Fruit yield/Plant (Normal) [gr]
18

HI [yield/yield + biomass] (Low N)
19

HI [yield/yield + biomass] (Normal)
20

Leaflet Length [cm] (Low N)
21

Leaflet Length [cm] (Normal)
22

Leaflet Length [cm]) (Drought)
23

Leaflet Width (Low N) [cm]
24

Leaflet Width (Normal) [cm]
25

Leaflet Width [cm] (Drought)
26

NUE [yield/SPAD] (Low N)
27

NUE [yield/SPAD] (Normal)
28

NUE2 [total biomass/SPAD] (Low N)
29

NUE2 [total biomass/SPAD] (Normal)
30

NUpE [biomass/SPAD] (Low N)
31

NUpE [biomass/SPAD] (Normal)
32

No flowers (NUE) [num]
33

No flowers (Normal) [num]
34

Num of Flower Drought/NUE
35

Num of Flower Drought/Normal
36

Num of flowers (Drought) [num]
37

Num. Flowers NUE/Normal
38

RWC (Normal) [%]
39

RWC Drought [%]
40

RWC Drought/Normal
41

RWC NUE [%]
42

RWC NUE/Normal
43

SAPD 100% RWC NUE/Normal
44

SLA [leaf area/plant biomass] (Low N)
45

SLA [leaf area/plant biomass] (Normal)
46

SPAD (Normal) (SPAD unit)
47

SPAD 100% RWC (NUE)
48

SPAD 100% RWC (Normal)
49

SPAD NUE (SPAD unit)
50

SPAD NUE/Normal
51

Total Leaf Area [cm²] (Low N)
52

Total Leaf Area [cm²] (Normal)
53

Total Leaf Area [cm²]) (Drought)
54

Weight Flower clusters (Normal)
55

Weight clusters (flowers) (NUE)
56

Weight flower clusters (Drought)
57

Yield/SLA (Low N)
58

Yield/SLA (Normal)
59

Yield/total leaf area (Low N)
60

Yield/total leaf area (Normal)
61

average red fruit weight (NUE) [gr]
62

average red fruit weight (Normal) [gr]
63

average red fruit weight Drought [gr]
64

flower cluster weight Drought/NUE
65

flower cluster weight Drought/Normal
66

red fruit weight Drought/Normal
67

Table 115. Provided are the tomato correlated parameters. “gr.” = grams; “FW” = fresh weight; “NUE” = nitrogen use efficiency; “RWC” = relative water content; “NUpE” = nitrogen uptake efficiency; “SPAD” = chlorophyll levels; “HI” = harvest index (vegetative weight divided on yield); “SLA” = specific leaf area (leaf area divided by leaf dry weight).

Fruit Yield (grams)—At the end of the experiment [when 50% of the fruit were ripe (red)] all fruits from plots within blocks A-C were collected. The total fruits were counted and weighted. The average fruits weight was calculated by dividing the total fruit weight by the number of fruits.

Yield/SLA—Fruit yield divided by the specific leaf area, gives a measurement of the balance between reproductive and vegetative processes.

Yield/total leaf area—Fruit yield divided by the total leaf area, gives a measurement of the balance between reproductive and vegetative processes.

Plant Fresh Weight (grams)—At the end of the experiment [when 50% of the fruit were ripe (red)] all plants from plots within blocks A-C were collected. Fresh weight was measured (grams).

Inflorescence Weight (grams)—At the end of the experiment [when 50% of the fruits were ripe (red)] two inflorescence from plots within blocks A-C were collected. The inflorescence weight (gr.) and number of flowers per inflorescence were counted.

Water use efficiency (WUE)—can be determined as the biomass produced per unit transpiration. To analyze WUE, leaf relative water content was measured in control and transgenic plants. Fresh weight (FW) was immediately recorded; then leaves were soaked for 8 hours in distilled water at room temperature in the dark, and the turgid weight (TW) was recorded. Total dry weight (DW) was recorded after drying the leaves at 60° C. to a constant weight. Relative water content (RWC) was calculated according to the following Formula I [(FW−DW/TW−DW)×100] as described above.

Plants that maintain high relative water content (RWC) compared to control lines were considered more tolerant to drought than those exhibiting reduced relative water content

Experimental Results

TABLE 116

Measured parameters in Tomato accessions under drought conditions

Line/Corr. ID
1
4
9
12
13
15
17
23
26
35

Line-1

1.72
2.62
1.15
0.57
0.47

0.88

Line-2

0.34
1.09
0.73
1.41
0.48

1.22

Line-3

0.61
1.85
1.32
1.27
0.63

1.74

Line-4

2.63
2.22
0.76
2.88
0.35

1.56

Line-5

1.18
2.63
1.51
4.20
2.04

1.09

Line-6

1.36
2.71
0.71
0.55
0.25

1.52

Line-7

4.02
3.41
5.06
0.09
0.05

4.96

Line-8

1.01
2.11
0.89
1.03
0.45

1.08

Line-9

0.61
1.95
0.67
1.39
0.29

0.98

Line-10

0.64
1.76
2.17
3.28
1.02

4.94

Line-11

0.95
1.72
0.38
0.91
0.60

0.88

Line-12
0.80
0.89
0.51
1.92
1.27
2.62
0.49
5.15
2.55
0.79

Line-13
0.28
0.35
1.17
2.21
0.84
0.32
0.27
3.38
2.04
2.12

Line-14
0.38
0.63
1.94
3.73
1.51
2.48
0.68
7.14
4.17
1.29

Line-15
0.63
2.27
0.35
0.75
0.98
0.41
0.14
5.48
3.09
1.61

Line-16
2.86
7.40
1.06
1.76
1.34
1.62
0.53
8.62
4.69
1.90

Line-17
1.16
2.94
0.21
0.63
0.38
1.76
0.55
6.35
3.87
1.36

Line-18
4.40
11.60
0.48
1.11
0.84
1.42
0.41
6.77
2.91
1.42

Table 116: Provided are the values of each of the parameters (as described above) measured in Tomato accessions (Seed ID) under drought conditions. Growth conditions are specified in the experimental procedure section.

TABLE 117

Additional Measured parameters in Tomato accessions

under drought conditions

Line/

Corr. ID
36
37
40
41
54
57

Line-1
2.94
16.67
72.12
0.99

0.37

Line-2
0.34
6.50
74.51
0.97

0.41

Line-3
2.47
15.67
65.33
1.02

0.33

Line-4
2.65
20.33
72.22
1.08

0.29

Line-5
1.21
11.67
66.13
1.21

0.55

Line-6
3.04
25.33
68.33
0.88

0.31

Line-7
5.95
29.73
78.13
1.34

0.45

Line-8
2.08
17.33
18.46
0.28

0.56

Line-9
1.47
14.67
73.21
1.13

0.30

Line-10
4.24
29.67
62.50
0.83

0.31

Line-11
1.67
15.00
67.21
1.01

0.31

Line-12
1.29
10.33
75.76
1.20
337.63
0.31

Line-13
3.44
18.33
62.82
1.11
130.78
8.36

Line-14
1.50
12.00
70.69
1.97
557.93
0.29

Line-15
2.65
20.33
55.75
0.72
176.67
0.34

Line-16
1.41
12.67
75.22
0.75
791.86
0.44

Line-17
1.19
12.67
63.68
1.01
517.05
0.27

Line-18
1.26
11.33
62.31
0.83
832.27
0.43

Table 117. Provided are the values of each of the parameters (as described above) measured in Tomato accessions (Seed ID) under drought conditions. Growth conditions are specified in the experimental procedure section.

TABLE 118

Measured parameters in Tomato accessions under normal conditions

Line/

Correlation ID
3
6
11
18
20
22
25
28
30
32

Line-1

1.53
0.83
0.35

0.017
0.05
0.03

Line-2

3.17
0.34
0.10

0.009
0.09
0.09

Line-3
0.56
0.82
3.02
0.49
0.14
6.34
3.69
0.009
0.06
0.05

Line-4
3.05
2.46
0.84
0.12
0.12
7.99
4.77
0.003
0.02
0.02

Line-5
0.24
0.50
2.24
0.49
0.18
5.59
3.43
0.010
0.06
0.05

Line-6
2.58
2.76
1.98
0.45
0.19
7.70
4.56
0.010
0.06
0.05

Line-7
6.32
5.32
0.85
0.53
0.38
7.85
4.44
0.012
0.03
0.02

Line-8
5.75
5.24
2.09
0.44
0.17
6.22
3.15
0.008
0.05
0.04

Line-9
0.38
0.61
3.21
0.21
0.06
6.16
3.37
0.004
0.06
0.05

Line-10
0.30
0.66
2.75
0.31
0.10
5.65
3.13
0.006
0.06
0.05

Line-11
1.95
2.70
1.81
0.66
0.27
4.39
2.40
0.017
0.06
0.05

Line-12
2.53
0.70
3.77
0.19
0.05
4.44
2.02
0.004
0.08
0.08

Line-13
1.42
2.64
1.89
0.85
0.31
6.77
3.80
0.015
0.05
0.03

Line-14
2.03
4.67
1.93
0.27
0.12
7.42
3.74
0.006
0.05
0.04

Line-15
1.39
2.17
2.14
0.35
0.14
6.71
2.98
0.008
0.06
0.05

Line-16
2.27
0.49
1.65
0.33
0.17
5.87
3.22
0.006
0.04
0.03

Line-17
0.45
0.34
3.01
0.31
0.09
4.16
2.09
0.008
0.08
0.07

Line-18
0.42
0.75
2.29
0.29
0.11
10.29
5.91
0.005
0.04
0.04

Table 118: Provided are the values of each of the parameters (as described above) measured in Tomato accessions (Seed ID) under normal growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 119

Additional measured parameters in Tomato accessions under normal conditions

Line/

Corr. ID
34
39
46
47
49
53
55
59
61
63

Line-1
5.67
72.83

49.70
36.17

1.17

0.05

Line-2
19.33
76.47

37.20
28.45

0.34

0.01

Line-3
6.33
64.29
140.99
55.80
35.89
426.1
0.69
0.0035
0.0012
0.01

Line-4
7.67
67.07
689.67
46.40
31.09
582.38
56.35
0.0002
0.0002
0.29

Line-5
9.67
54.79
130.22
48.20
26.38
291.40
0.44
0.0037
0.0017
0.01

Line-6
8.33
77.61
299.12
43.0
33.68
593.58
11.31
0.0015
0.0008
0.05

Line-7
5.00
58.18
1117.74
42.90
24.98
947.59
0.79
0.0005
0.0006
0.23

Line-8
8.33
66.51
111.77
53.30
35.47
233.35
0.58
0.0039
0.0019
0.29

Line-9
10.00
64.7
106.29
58.50
37.87
340.73
0.73
0.0020
0.0006
0.01

Line-10
7.00
75.25
123.14
51.10
38.43
339.11
0.83
0.0025
0.0009
0.01

Line-11
9.00
66.23
104.99
40.00
26.49
190.14
0.86
0.0063
0.0035
0.06

Line-12
8.00
63.21
111.88
47.60
30.07
421.79
0.50
0.0017
0.004
0.01

Line-13
5.33
56.77
307.95
57.90
32.89
581.33
1.02
0.0028
0.0015
0.03

Line-14
8.00
35.96
419.37
48.30
17.35
807.51
0.70
0.0007
0.0003
0.26

Line-15
7.67
77.62
365.81
43.60
33.82
784.06
0.38
0.0009
0.0004
0.03

Line-16
9.00
100.00
212.93
54.50
54.47
351.80
0.66
0.0015
0.0009
0.00

Line-17
10.67
63.16
84.94
41.60
26.25
255.78
0.70
0.0037
0.0012
0.00

Line-18
9.00
75.13
469.87
59.10
44.43
1078.10
0.33
0.0006
0.0003
0.01

Table 119: Provided are the values of each of the parameters (as described above) measured in Tomato accessions (Seed ID) under normal growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 120

Measured parameters in Tomato accessions under low nitrogen conditions

Line/

Corr. ID
2
5
7
8
10
14
16
19
21
24

Line-1
0.87
1.06
0.46
2.65
4.04
0.49
0.41
0.09
6.40
3.47

Line-2
3.66
6.87
1.07
0.38
1.21
1.93
0.66
0.35
5.92
1.97

Line-3
0.57
0.65
0.44
0.74
2.25
0.97
0.48
0.18
3.69
1.79

Line-4
0.37
0.53
0.01
3.01
2.54
3.80
0.46
0.15
5.43
2.55

Line-5
3.40
7.17
1.08
0.83
1.85
2.78
1.35
0.42
6.95
3.52

Line-6
0.68
0.44
0.02
1.54
3.06
0.78
0.35
0.10
3.73
1.73

Line-7
0.45

0.37
3.70
3.13
0.02
0.01
0.00
4.39
1.87

Line-8
0.47
0.55
0.81
1.22
2.54
1.16
0.51
0.17
6.72
3.54

Line-9
0.54
0.75
0.55
0.58
1.84
2.07
0.44
0.19
6.66
3.28

Line-10
0.39
0.58
0.36
0.55
1.52
1.51
0.47
0.24
4.39
2.52

Line-11
0.97
1.27
0.95
1.06
1.91
2.41
1.59
0.45
3.90
2.61

Line-12
0.91
1.34
0.80
0.49
1.86
2.06
0.39
0.17
5.29
2.61

Line-13
0.36
0.52
0.34
1.31
2.47
0.38
0.32
0.12
6.32
3.58

Line-14
0.35
0.57
0.61
1.36
2.62
1.64
0.45
0.15
5.11
2.56

Line-15
0.57
0.94
0.94
0.51
1.08
0.41
0.14
0.12
4.72
2.48

Line-16
4.38
6.17
0.68
0.71
1.17
1.21
0.40
0.25
6.83
3.43

Line-17
2.02
3.67
0.40
0.31
0.92
4.59
1.44
0.61
7.10
3.30

Line-18
8.13
11.33
1.44
0.47
1.09
1.70
0.50
0.31
8.21
3.69

Table 120: Provided are the values of each of the parameters (as described above) measured in Tomato accessions (Seed ID) under low nitrogen growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 121

Additional measured parameters in Tomato accessions under low nitrogen conditions

Line/Corr. ID
27
29
31
33
38
42
43
44
45
48

Line-1
0.01
0.16
0.14
19.0
3.35
74.07
1.02
0.79
140.04
28.47

Line-2
0.02
0.05
0.03
5.33
0.28
99.08
1.30
1.37
317.12
39.04

Line-3
0.01
0.08
0.07
9.00
1.42
69.49
1.08
0.92
131.29
33.01

Line-4
0.02
0.13
0.11
13.00
1.70
63.24
0.94
0.75
148.82
23.42

Line-5
0.04
0.09
0.05
10.67
1.10
77.36
1.41
1.31
257.51
34.53

Line-6
0.01
0.11
0.09
16.67
2.00
77.91
1.00
0.97
264.34
32.51

Line-7
0.00
0.11
0.11
6.00
1.20
80.49
1.38
1.11
144.60
27.66

Line-8
0.02
0.09
0.08
16.00
1.92
67.40
1.01
0.95
246.05
33.68

Line-9
0.01
0.08
0.06
15.00
1.50
67.16
1.04
0.79
405.55
30.04

Line-10
0.01
0.06
0.04
6.00
0.86
66.07
0.88
0.92
299.32
35.50

Line-11
0.06
0.14
0.08
17.00
1.89
69.57
1.05
0.94
86.19
24.81

Line-12
0.01
0.06
0.05
13.00
1.63
69.30
1.10
1.16
182.32
40.77

Line-13
0.01
0.06
0.05
8.67
1.63
100.00
1.76
1.44
160.18
47.47

Line-14
0.02
0.12
0.10
9.33
1.17
57.66
1.60
1.50
90.10
26.06

Line-15
0.00
0.03
0.03
12.67
1.65
90.79
1.17
1.05
160.99
35.38

Line-16
0.01
0.05
0.04
6.67
0.74
68.00
0.68
0.56
379.03
30.60

Line-17
0.04
0.06
0.02
9.33
0.88
59.65
0.94
1.48
531.08
38.97

Line-18
0.01
0.04
0.03
8.00
0.89
72.17
0.96
0.84
650.68
37.46

Table 121: Provided are the values of each of the parameters (as described above) measured in Tomato accessions (Seed ID) under low nitrogen growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 122

Additional measured parameters in Tomato accessions under low nitrogen conditions

Line/

Correlation ID
50
51
52
56
58
60
62

Line-1
38.40
0.77
565.93
0.53
0.0029
0.0007
0.02

Line-2
39.40
1.06
384.77
0.37
0.0021
0.0017
0.19

Line-3
47.50
0.85
294.83
0.31
0.0036
0.0016
0.01

Line-4
37.00
0.80
378.00
0.35
0.0031
0.0012
0.01

Line-5
44.60
0.93
476.39
0.47
0.0052
0.0028
0.10

Line-6
41.70
0.96
197.08
0.25
0.0055
0.0018
0.00

Line-7
34.40
0.80
453.24
0.29
0.0001
0.0000
0.01

Line-8
50.00
0.94
625.51
0.47
0.0021
0.0008
0.01

Line-9
44.70
0.76
748.01
0.40
0.0011
0.0006
0.01

Line-10
53.70
1.05
453.96
0.30
0.0016
0.0010
0.01

Line-11
35.70
0.89
164.85
0.82
0.0185
0.0097
0.02

Line-12
58.80
1.24
338.30
0.40
0.0021
0.0011
0.01

Line-13
47.50
0.82
396.00
0.35
0.0020
0.0008
0.01

Line-14
45.20
0.94
236.15
0.43
0.0050
0.0019
0.05

Line-15
39.00
0.89
174.58
0.35
0.0009
0.0008
0.36

Line-16
45.00
0.83
441.78
0.45
0.0010
0.0009
0.04

Line-17
65.30
1.57
489.18
0.28
0.0027
0.0029
0.63

Line-18
51.90
0.88
707.80
0.47
0.0008
0.0007

Table 122: Provided are the values of each of the parameters (as described above) measured in Tomato accessions (Seed ID) under low nitrogen growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 123

Correlation between the expression level of selected LAB

genes of some embodiments of the invention in various tissues

and the phenotypic performance under low nitrogen,

normal or drought stress conditions across Tomato accessions

Gene

Exp.
Corr.

Name
R
P value
set
Set ID

LAB824
0.74
2.13E−02
11
32

LAB824
0.79
1.05E−02
11
30

LAB824
0.84
2.56E−03
10
19

LAB824
0.72
1.84E−02
10
45

LAB824
0.83
2.69E−03
4
14

LAB824
0.83
2.69E−03
4
51

LAB824
0.89
1.43E−03
4
62

LAB824
0.75
1.32E−02
4
50

LAB824
0.78
7.80E−03
9
2

LAB824
0.71
2.24E−02
9
5

LAB825
0.73
1.60E−02
3
42

LAB825
0.71
2.09E−02
1
34

LAB827
0.72
1.80E−02
3
33

LAB827
0.78
7.77E−03
2
63

LAB827
0.71
2.19E−02
10
31

LAB827
0.84
2.20E−03
1
63

LAB827
0.78
7.28E−03
6
9

LAB827
0.81
4.93E−03
4
33

LAB827
0.74
1.50E−02
5
12

LAB827
0.81
4.16E−03
5
9

LAB829
0.93
1.05E−04
3
48

LAB829
0.79
6.65E−03
3
44

LAB829
0.79
1.15E−02
11
20

LAB829
0.70
3.39E−02
12
20

LAB829
0.81
1.45E−02
12
3

LAB829
0.83
1.10E−02
12
6

LAB830
0.73
1.66E−02
3
48

LAB830
0.76
1.78E−02
11
20

LAB830
0.78
1.29E−02
11
28

LAB830
0.81
1.56E−02
12
59

LAB830
0.76
2.91E−02
12
61

LAB830
0.72
2.01E−02
10
5

LAB830
0.86
1.53E−03
6
67

LAB830
0.76
1.14E−02
6
64

LAB830
0.81
4.61E−03
4
42

LAB831
0.87
1.21E−03
10
2

LAB831
0.82
3.61E−03
10
5

LAB831
0.84
2.36E−03
4
7

LAB831
0.75
1.24E−02
5
35

LAB832
0.77
1.42E−02
3
62

LAB832
0.88
1.87E−03
11
20

LAB832
0.90
8.37E−04
11
28

LAB832
0.74
3.52E−02
12
22

LAB832
0.71
3.08E−02
12
20

LAB832
0.73
2.62E−02
12
28

LAB832
0.74
3.47E−02
12
25

LAB833
0.72
2.92E−02
11
32

LAB833
0.72
3.03E−02
11
30

LAB833
0.75
3.15E−02
12
59

LAB833
0.81
1.48E−02
12
61

LAB833
0.78
7.37E−03
10
58

LAB834
0.73
1.57E−02
3
44

LAB834
0.75
1.28E−02
10
2

LAB834
0.77
9.91E−03
10
5

LAB834
0.73
1.69E−02
4
56

LAB834
0.76
1.05E−02
4
7

LAB835
0.72
1.79E−02
6
35

LAB835
0.84
2.24E−03
5
35

LAB836
0.77
1.42E−02
11
28

LAB836
0.71
4.70E−02
12
6

LAB836
0.71
2.01E−02
2
47

LAB836
0.75
1.27E−02
10
19

LAB836
0.72
2.00E−02
10
5

LAB836
0.71
2.23E−02
5
67

LAB837
0.82
3.47E−03
10
58

LAB839
0.76
2.94E−02
12
59

LAB839
0.72
4.29E−02
12
61

LAB839
0.79
6.69E−03
4
50

LAB839
0.79
6.43E−03
5
64

LAB842
0.76
1.01E−02
5
35

LAB843
0.77
9.49E−03
3
44

Table 123. ″Corr. ID″ — correlation set ID according to the correlated parameters Table above.

″Exp. Set ID″ = Expression set.

″R″ = Pearson correlation coefficient;

″P″ = p value.

II. Correlation of early vigor traits across collection of Tomato ecotypes under 300 mM NaCl, low nitrogen and normal growth conditions—Ten tomato hybrids were grown in 3 repetitive plots, each containing 17 plants, at a net house under semi-hydroponics conditions. Briefly, the growing protocol was as follows: Tomato seeds were sown in trays filled with a mix of vermiculite and peat in a 1:1 ratio. Following germination, the trays were transferred to the high salinity solution (300 mM NaCl in addition to the Full Hoagland solution), low nitrogen solution (the amount of total nitrogen was reduced in a 90% from the full Hoagland solution, final amount of 0.8 mM N), or at Normal growth solution (Full Hoagland containing 8 mM N solution, at 28±2° C.). Plants were grown at 28±2° C.

Analyzed tomato tissues—All 10 selected Tomato varieties were sample per each treatment. Two types of tissues [leaves and roots] were sampled and RNA was extracted as described above. For convenience, each micro-array expression information tissue type has received a Set ID as summarized in Table 124 below.

TABLE 124

Tomato transcriptom expression sets

Expression Set
Set IDs

Leaf - normal conditions
1 + 10

Root - normal conditions
2 + 9

Leaf - low nitrogen conditions
3 + 8

Root - low nitrogen conditions
4 + 7

Leaf - salinity conditions
5 + 12

Root - salinity conditions
6 + 11

Table 124. Provided are the tomato transcriptom experimental sets.

Tomato vigor related parameters—following 5 weeks of growing, plant were harvested and analyzed for leaf number, plant height, chlorophyll levels (SPAD units), different indices of nitrogen use efficiency (NUE) and plant biomass. Next, analyzed data was saved to text files and processed using the JMP statistical analysis software (SAS institute). Data parameters collected are summarized in Table 125, herein below.

Leaf number—number of opened leaves.

RGR Leaf Num—calculated relative growth rate (RGR) of leaf number.

Shoot/Root—biomass of shoot divided by biomass of roots.

NUE total biomass—nitrogen use efficiency (NUE) calculated as total biomass divided by nitrogen concentration.

NUE root biomass—nitrogen use efficiency (NUE) of root growth calculated as root biomass divided by nitrogen concentration NUE shoot biomass—nitrogen use efficiency (NUE) of shoot growth calculated as shoot biomass divided by nitrogen concentration. Percent of reduction of root biomass compared to normal—the difference (reduction in percent) between root biomass under normal and under low nitrogen conditions.

Percent of reduction of shoot biomass compared to normal—the difference (reduction in percent) between shoot biomass under normal and under low nitrogen conditions.

Percent of reduction of total biomass compared to normal—the difference (reduction in percent) between total biomass (shoot and root) under normal and under low nitrogen conditions.

SPAD [SPAD unit]—Chlorophyll content was determined using a Minolta SPAD 502 chlorophyll meter and measurement was performed 64 days post sowing. SPAD meter readings were done on young fully developed leaf. Three measurements per leaf were taken per plot.

Root Biomass [DW, gr.]/SPAD—root biomass divided by SPAD results.

Shoot Biomass [DW, gr.]/SPAD—shoot biomass divided by SPAD results.

Total Biomass (Root+Shoot) [DW, gr.]/SPAD—total biomass divided by SPAD results.

TABLE 125

Tomato correlated parameters (vectors)

Correlated parameter with
Correlation ID

Leaf number Ratio Low N/Normal
1

Leaf number Ratio NaCl/Normal
2

Leaf number Ratio NaCl/Low N
3

N level/Leaf [SPAD unit/leaf]
4

NUE roots (Root Biomass [DW]/SPAD) (gr/SPAD unit)
5

NUE shoots (shoot Biomass [DW]/SPAD)
6

(gr/SPAD unit)

NUE total biomass (Total Biomass [DW]/SPAD)
7

(gr/SPAD unit)

Percent of reduction of root biomass compared to
8

normal (%)

Percent of reduction of shoot biomass compared to
9

normal (%)

Plant Height Low N/Normal
10

Plant Height Ratio NaCl/Low N
11

Plant Height Ratio NaCl/Normal
12

Plant biomass NaCl (gr)
13

Plant height Low N (cm)
14

Plant height NaCl (cm)
15

Plant height Normal (cm)
16

NUE Root Biomass [DW]/SPAD (gr/SPAD unit)
17

SPAD Ratio Low N/Normal
18

SPAD Low N (SPAD unit)
19

SPAD Normal (SPAD unit)
20

NUE Shoot Biomass [DW]/SPAD (gr/SPAD unit)
21

Shoot/Root
22

NUE Total Biomass [Root + Shoot DW]/SPAD
23

(gr/SPAD unit)

Plant height Normal (cm)
24

leaf number Low N (num)
25

leaf number Normal (num)
26

leaf number NaCl (num)
27

Table 125. Provided are the tomato correlated parameters. “NUE” = nitrogen use efficiency; “DW” = dry weight; “cm” = centimeter;

Experimental Results

10 different Tomato varieties were grown and characterized for parameters as described above (Table 125). The average for each of the measured parameter was calculated using the JMP software and values are summarized in Tables 126-129 below. Subsequent correlation analysis was conducted (Table 130). Follow, results were integrated to the database.

TABLE 126

Measured parameters in Tomato accessions under low nitrogen conditions

Line/Corr.

ID
1
4
5
6
7
8
9
10
14
17
18

Line-1
0.85
10.854
6.99
35.35
58.4
62.592
75.38
0.81
36.78
0.0008
1.01

Line-2
0.9
11.409
2.54
24.09
63.75
54.158
55.112
0.83
39.89
0.0003
0.98

Line-3
0.98

0.84
34.44

1.02

Line-4
1.09
10.438
7.04
65.02
69.29
70.547
49.726
0.85
47
0.0008
1

Line-5
0.88
11.169
5.04
46.71
71.1
59.685
63.189
0.83
46.44
0.0005
0.98

Line-6
1.02
8.9286
8.01
46.67
60.54
96.129
82.667
0.93
45.44
0.0009
0.98

Line-7
0.87
7.9264
15.09
120.07
73.9
106.5
66.924
0.85
47.67
0.0014
0.93

Line-8
1.06
7.9932
9.02
60.09
68.81
111.9
107.98
1.05
39.33
0.001
1.05

Line-9
0.91
10.304
8.78
66.27
66.74
81.644
55.401
0.84
41.78
0.0009
1.01

Line-10
1.12
8.5852
7.25
56.46
70.82
32.214
54.433
0.88
41
0.0009
0.99

Line-11

11.528
7.73
38.35
69.7
143.71
62.155

0.0008

Line-12

14.491
15.94
60.32
49.72
87.471
59.746

0.0015

Table 126. Provided are the values of each of the parameters (as descnbed above) measured in Tomato accessions (Line) under low nitrogen growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 127

Additional measured parameters in Tomato accessions

under low nitrogen conditions

Line/

Corr. ID
19
21
22
23
24
25

Line-1
34.57
0.0041
5.0104
0.005
45.33
5.56

Line-2
24.87
0.003
11.393
0.0034
47.78
6.22

Line-3
28.58

40.78
7.22

Line-4
31.58
0.0072
9.4941
0.008
55.33
6.78

Line-5
29.72
0.0049
11.6
0.0055
56.22
5.56

Line-6
31.83
0.0052
8.2001
0.006
48.67
6.56

Line-7
30.33
0.0115
10.375
0.0129
55.78
5.11

Line-8
30.29
0.0069
10.523
0.0079
37.44
5.89

Line-9
31.32
0.0068
8.2421
0.0077
49.56
5.56

Line-10
28.77
0.0067
7.9668
0.0076
46.33
6.33

Line-11

0.0042
6.4137
0.005

Line-12

0.0056
3.9092
0.007

Table 127. Provided are the values of each of the parameters (as described above) measured in Tomato accessions (Line) under low nitrogen growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 128

Measured parameters in Tomato accessions under normal conditions

Corr. ID

Line
4
5
6
7
16
17
20
21
22
23
26

Line-1
9.29
1.12
4.69
7.47
45.33
0.00
34.30
0.01
5.40
0.006
6.56

Line-2
8.87
0.47
4.37
8.63
47.78
0.00
25.31
0.01
10.02
0.006
6.89

Line-3

40.78

28.12

7.33

Line-4
8.43
1.00
13.08
8.85
55.33
0.00
31.43
0.01
15.42
0.015
6.22

Line-5
9.83
0.84
7.39
7.22
56.22
0.00
30.24
0.01
8.83
0.009
6.33

Line-6
8.57
0.83
5.65
7.87
48.67
0.00
32.43
0.01
7.52
0.006
6.44

Line-7
6.57
0.94
17.94
9.09
55.78
0.00
32.58
0.02
12.61
0.019
5.89

Line-8
6.97
0.81
5.56
7.91
37.44
0.00
28.77
0.01
7.99
0.008
5.56

Line-9
8.71
1.08
11.96
8.55
49.56
0.00
30.92
0.01
14.31
0.012
6.11

Line-10
7.35
2.25
10.37
8.68
46.33
0.00
28.99
0.01
4.80
0.014
5.67

Line-11
10.18
0.54
6.17
9.10

0.00

0.01
12.65
0.007

Line-12
9.37
1.82
10.10
6.24

0.00

0.01
6.29
0.011

Table 128. Provided are the values of each of the parameters (as described above) measured in Tomato accessions (Line) under normal growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 129

Measured parameters in Tomato accessions under salinity conditions

Corr. ID

Line
2
3
4
11
12
13
15
17
21
23
27

Line-1
0.54
0.64
11.4
0.15
0.12
0.36
5.6
6E−05
0.0005
0.0007
3.56

Line-2
0.57
0.63
11.639
0.16
0.14
0.44
6.46
0.0001
0.0007
0.0008
3.94

Line-3
0.68
0.69

0.25
0.21
0.26
8.47

5

Line-4
0.64
0.59
10.788
0.18
0.15
0.71
8.56
1E−04
0.0012
0.0014
4

Line-5
0.56
0.64
10.776
0.19
0.16
0.46
8.87
7E−05
0.0017
0.0018
3.56

Line-6
0.68
0.67
6.9524
0.17
0.16
0.54
7.56
9E−05
0.001
0.0011
4.39

Line-7
0.54
0.62
9.2128
0.18
0.15
0.66
8.64
1E−04
0.0012
0.0013
3.17

Line-8
0.67
0.63
8.5376
0.14
0.15
0.4
5.57
8E−05
0.0007
0.0008
3.72

Line-9
0.65
0.72
10.37
0.14
0.12
0.52
5.82
9E−05
0.001
0.0011
4

Line-10
0.75
0.68
8.8395
0.23
0.2
0.45
9.36

0.001

4.28

Line-11

10.434

5E−05
0.0007
0.0006

Line-12

12.429

5E−05
0.0007
0.0007

Table 129. Provided are the values of each of the parameters (as described above) measured in Tomato accessions (Line) under salinity growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 130

Correlation between the expression level of selected LAB genes of some embodiments

of the invention in various tissues and the phenotypic performance under low

nitrogen, normal or salinity stress conditions across Tomato accessions

Gene

Exp.
Corr.
Gene

Exp.
Corr.

Name
R
P value
set
Set ID
Name
R
P value
set
Set ID

LAB824
0.72
2.80E−02
1
22
LAB824
0.75
3.33E−02
4
25

LAB824
0.81
1.46E−02
6
23
LAB824
0.80
5.90E−03
6
15

LAB824
0.76
1.70E−02
5
21
LAB824
0.75
3.18E−02
5
23

LAB825
0.79
1.09E−02
4
9
LAB827
0.73
1.72E−02
5
11

LAB830
0.82
7.15E−03
1
5
LAB830
0.82
6.57E−03
1
17

LAB830
0.91
2.83E−04
5
13
LAB830
0.70
5.31E−02
5
17

LAB830
0.70
2.39E−02
6
2
LAB830
0.72
4.43E−02
3
10

LAB830
0.78
1.36E−02
3
9
LAB830
0.76
1.81E−02
2
17

LAB831
0.78
1.39E−02
4
6
LAB831
0.83
5.21E−03
4
5

LAB831
0.78
1.37E−02
4
23
LAB831
0.76
1.70E−02
4
21

LAB831
0.81
8.17E−03
4
17
LAB831
0.78
1.40E−02
3
7

LAB831
0.75
1.97E−02
7
5
LAB831
0.75
1.88E−02
7
17

LAB832
0.87
4.87E−03
1
16
LAB832
0.87
4.87E−03
3
24

LAB832
0.89
2.94E−03
3
14
LAB833
0.83
6.10E−03
4
6

LAB833
0.82
7.18E−03
4
5
LAB833
0.81
8.72E−03
4
23

LAB833
0.80
9.65E−03
4
21
LAB833
0.75
1.96E−02
4
17

LAB834
0.74
2.27E−02
3
7
LAB835
0.73
1.74E−02
6
13

LAB835
0.78
1.37E−02
6
21
LAB835
0.71
4.99E−02
6
23

LAB835
0.70
2.30E−02
6
27
LAB837
0.76
2.75E−02
4
18

LAB837
0.73
4.01E−02
3
25
LAB839
0.74
1.42E−02
6
27

LAB839
0.79
1.15E−02
3
8
LAB840
0.73
3.87E−02
1
26

LAB840
0.70
2.29E−02
6
15
LAB842
0.76
1.75E−02
1
22

LAB842
0.78
1.25E−02
10
22
LAB843
0.85
3.33E−03
9
5

LAB843
0.81
8.16E−03
9
17
LAB843
0.74
1.49E−02
6
27

LAB843
0.75
1.29E−02
6
11
LAB843
0.87
2.45E−03
2
5

LAB843
0.81
7.50E−03
2
17

Table 130. Correlations (R) between the genes expression levels in various tissues and the phenotypic performance. “Corr. ID”—correlation set ID according to the correlated parameters Table 125 above. “Exp. Set”—Expression set. “R” = Pearson correlation coefficient; “P” = p value.

Example 14
Production of Soybean (Glycine max) Transcriptom and High Throughput Correlation Analysis with Yield Parameters Using 44K B. Soybean Oligonucleotide Micro-Arrays

In order to produce a high throughput correlation analysis, the present inventors utilized a Soybean oligonucleotide micro-array, produced by Agilent Technologies [Hypertext Transfer Protocol://World Wide Web (dot) chem. (dot) agilent (dot) com/Scripts/PDS (dot) asp?1Page=50879]. The array oligonucleotide represents about 42,000 Soybean genes and transcripts. In order to define correlations between the levels of RNA expression with yield components or plant architecture related parameters or plant vigor related parameters, various plant characteristics of 29 different Glycine max varieties were analyzed and 12 varieties were further used for RNA expression analysis. The correlation between the RNA levels and the characterized parameters was analyzed using Pearson correlation test.

Experimental Procedures

Twenty nine Soybean varieties were grown in three repetitive plots, in field. Briefly, the growing protocol was as follows: Soybean seeds were sown in soil and grown under normal conditions until harvest.

Analyzed Soybean Tissues

In order to define correlations between the levels of RNA expression with yield components or plant architecture related parameters or vigor related parameters, 12 different Soybean varieties (out of 29 varieties) were analyzed and used for gene expression analyses as described above. Analysis was performed at two pre-determined time periods: at pod set (when the soybean pods are formed) and at harvest time (when the soybean pods are ready for harvest, with mature seeds). Each micro-array expression information tissue type has received a Set ID as summarized in Table 131 below.

TABLE 131

Soybean transcriptome expression sets

Expression Set
Set ID

Apical meristem at vegetative stage under normal growth
1

condition

Leaf at vegetative stage under normal growth condition
2

Leaf at flowering stage under normal growth condition
3

Leaf at pod setting stage under normal growth condition
4

Root at vegetative stage under normal growth condition
5

Root at flowering stage under normal growth condition
6

Root at pod setting stage under normal growth condition
7

Stem at vegetative stage under normal growth condition
8

Stem at pod setting stage under normal growth condition
9

Flower bud at flowering stage under normal growth condition
10

Pod (R3-R4) at pod setting stage under normal growth condition
11

Table 131. Provided are the soybean transcriptome expression sets.

RNA extraction—All 12 selected Soybean varieties were sampled. Plant tissues [leaf, root. Stem, Pod, apical meristem, Flower buds] growing under normal conditions were sampled and RNA was extracted as described above.

Soybean Yield Components and Vigor Related Parameters Assessment

The collected data parameters were as follows:

Main branch base diameter [mm] at pod set—the diameter of the base of the main branch (based diameter) average of three plants per plot.

Fresh weight [gr./plant] at pod set—total weight of the vegetative portion above ground (excluding roots) before drying at pod set, average of three plants per plot.

Dry weight [gr./plant] at pod set—total weight of the vegetative portion above ground (excluding roots) after drying at 70° C. in oven for 48 hours at pod set, average of three plants per plot.

Total number of nodes with pods on lateral branches [num/plant]—counting of nodes which contain pods in lateral branches at pod set, average of three plants per plot.

Number of lateral branches at pod set [num/plant]—counting number of lateral branches at pod set, average of three plants per plot.

Total weight of lateral branches at pod set [gr./plant]—weight all lateral branches at pod set, average of three plants per plot.

Total weight of pods on main stem at pod set [gr./plant]—weight all pods on main stem at pod set, average of three plants per plot.

Total number of nodes on main stem [num/plant]—count of number of nodes on main stem starting from first node above ground, average of three plants per plot.

Total number of pods with 1 seed on lateral branches at pod set [num/plant]—count the number of pods containing 1 seed in all lateral branches at pod set, average of three plants per plot.

Total number of pods with 2 seeds on lateral branches at pod set [num/plant]—count the number of pods containing 2 seeds in all lateral branches at pod set, average of three plants per plot.

Total number of pods with 3 seeds on lateral branches at pod set [num/plant]—count the number of pods containing 3 seeds in all lateral branches at pod set, average of three plants per plot.

Total number of pods with 4 seeds on lateral branches at pod set [num/plant]—count the number of pods containing 4 seeds in all lateral branches at pod set, average of three plants per plot.

Total number of pods with 1 seed on main stem at pod set [num/plant]—count the number of pods containing 1 seed in main stem at pod set, average of three plants per plot.

Total number of pods with 2 seeds on main stem at pod set [num/plant]—count the number of pods containing 2 seeds in main stem at pod set, average of three plants per plot.

Total number of pods with 3 seeds on main stem at pod set [num/plant]—count the number of pods containing 3 seeds in main stem at pod set, average of three plants per plot.

Total number of pods with 4 seeds on main stem at pod set [num/plant]—count the number of pods containing 4 seeds in main stem at pod set, average of three plants per plot.

Total number of seeds per plant at pod set [num/plant]—count number of seeds in lateral branches and main stem at pod set, average of three plants per plot.

Total number of seeds on lateral branches at pod set [num/plant]—count total number of seeds on lateral branches at pod set, average of three plants per plot.

Total number of seeds on main stem at pod set [num/plant]—count total number of seeds on main stem at pod set, average of three plants per plot.

Plant height at pod set [cm/plant]—total length from above ground till the tip of the main stem at pod set, average of three plants per plot.

Plant height at harvest [cm/plant]—total length from above ground till the tip of the main stem at harvest, average of three plants per plot.

Total weight of pods on lateral branches at pod set [gr./plant]—weight of all pods on lateral branches at pod set, average of three plants per plot.

Ratio of the number of pods per node on main stem at pod set—calculated in formula XV, average of three plants per plot.

Total number of pods on main stem/Total number of nodes on main stem, average of three plants per plot. Formula XV:

Ratio of total number of seeds in main stem to number of seeds on lateral branches—calculated in formula XVI, average of three plants per plot.

Total number of seeds on main stem at pod set/Total number of seeds on lateral branches at pod set. Formula XVI:

Total weight of pods per plant at pod set [gr./plant]—weight all pods on lateral branches and main stem at pod set, average of three plants per plot.

Days till 50% flowering [days]—number of days till 50% flowering for each plot.

Days till 100% flowering [days]—number of days till 100% flowering for each plot.

Maturity [days]—measure as 95% of the pods in a plot have ripened (turned 100% brown). Delayed leaf drop and green stems are not considered in assigning maturity. Tests are observed 3 days per week, every other day, for maturity. The maturity date is the date that 95% of the pods have reached final color. Maturity is expressed in days after August 31 [according to the accepted definition of maturity in USA, Descriptor list for SOYBEAN, Hypertext Transfer Protocol://World Wide Web (dot) ars-grin (dot) gov/cgi-bin/npgs/html/desclist (dot) pl?51].

Seed quality [ranked 1-5]—measure at harvest, a visual estimate based on several hundred seeds. Parameter is rated according to the following scores considering the amount and degree of wrinkling, defective coat (cracks), greenishness, and moldy or other pigment. Rating is 1-very good, 2-good, 3-fair, 4-poor, 5-very poor.

Lodging [ranked 1-5]—is rated at maturity per plot according to the following scores: 1-most plants in a plot are erected, 2-All plants leaning slightly or a few plants down, 3-all plants leaning moderately, or 25%-50% down, 4-all plants leaning considerably, or 50%-80% down, 5-most plants down. Note: intermediate score such as 1.5 are acceptable.

Seed size [gr.]—weight of 1000 seeds per plot normalized to 13% moisture, measure at harvest.

Total weight of seeds per plant [gr./plant]—calculated at harvest (per 2 inner rows of a trimmed plot) as weight in grams of cleaned seeds adjusted to 13% moisture and divided by the total number of plants in two inner rows of a trimmed plot.

Yield at harvest [bushels/hectare]—calculated at harvest (per 2 inner rows of a trimmed plot) as weight in grams of cleaned seeds, adjusted to 13% moisture, and then expressed as bushels per acre.

Average lateral branch seeds per pod [number]—Calculate Num of Seeds on lateral branches-at pod set and divide by the Number of Total number of pods with seeds on lateral branches-at pod set.

Average main stem seeds per pod [number]—Calculate Total Number of Seeds on main stem at pod set and divide by the Number of Total number of pods with seeds on main stem at pod setting.

Main stem average internode length [cm]—Calculate Plant height at pod set and divide by the Total number of nodes on main stem at pod setting.

Total num of pods with seeds on main stem [number]—count all pods containing seeds on the main stem at pod setting.

Total num of pods with seeds on lateral branches [number]—count all pods containing seeds on the lateral branches at pod setting.

Total number of pods per plant at pod set [number]—count pods on main stem and lateral branches at pod setting.

Data parameters collected are summarized in Table 132, herein below

TABLE 132

Soybean correlated parameters (vectors)

Correlated parameter with
Correlation ID

100 percent flowering (days)
1

50 percent flowering (days)
2

Base diameter at pod set (mm)
3

DW at pod set (gr/plant)
4

Lodging (ranked 1-5)
5

Maturity (days)
6

Num of lateral branches (num)
7

Num of pods with 1 seed on main stem at pod set (num)
8

Num of pods with 2 seed on main stem (num)
9

Num of pods with 3 seed on main stem (num)
10

Num of pods with 4 seed on main stem (num)
11

Plant height at harvest (cm)
12

Plant height at pod set (cm)
13

Ratio number of pods per node on main stem
14

Ratio number of seeds per main stem to seeds per 1
15

Seed quality (ranked 1-5)
16

Seed size (gr)
17

Total Number of Seeds on lateral branches (num)
18

Total Number of Seeds on main stem at pod set (num)
19

Total no of pods with 1 seed on lateral branch (num)
20

Total no of pods with 2 seed on lateral branch (num)
21

Total no of pods with 3 seed on lateral branch (num)
22

Total no of pods with 4 seed on lateral branch (num)
23

Total number of nodes on main stem (num)
24

Total number of nodes with pods on lateral branch (num)
25

Total number of seeds per plant (num)
26

Total weight of lateral branches at pod set (gr/plant)
27

Total weight of pods on lateral branches (gr/plant)
28

Total weight of pods on main stem at pod set (gr/plant)
29

Total weight of pods per plant (gr/plant)
30

Total weight of seeds per plant (gr/plant)
31

fresh weight at pod set (gr/plant)
32

yield at harvest (bushels/hectare)
33

Average lateral branch seeds per pod (num)
34

Average main stem seeds per pod (num)
35

Main stem average internode length (cm)
36

Num pods with seeds on lateral branches-at pod
37

set (num)

Total number of pods per plant at pod set (num)
38

Total number of pods with seeds on main stem at pod set
39

(num)

Seed size (gr)
40

Table 132. Provided are the soybean correlated parameters. “DW” = dry weight;

Experimental Results

The different soybean accessions were grown and characterized for different parameters as described above. Table 132 describes the soybean correlated parameters. The average for each of the measured parameter was calculated using the JMP software and values are summarized in Tables 133-134 below. Subsequent correlation analysis between the various transcriptom sets and the average parameters (Table 135) was conducted. Follow, results were integrated to the database.

TABLE 133

Measured parameters in Soybean varieties (lines 1-6)

Line

Corr. ID
Line-1
Line-2
Line-3
Line-4
Line-5
Line-6

1
67.33
71.67
67.67
67.33
60
74

2
61
65.33
60.67
61
54.67
68.33

3
8.333
9.544
9.678
8.111
8.822
10.12

4
53.67
50.33
38
46.17
60.83
55.67

5
1.667
1.833
1.167
1.667
2.667
2.833

6
24
43.67
30.33
30.33
38.33
40

7
9
8.667
9.111
9.889
7.667
17.56

8
1.111
4.375
1.444
1.444
4.556
1.667

9
16.89
16.25
13.22
16.89
27
8.111

10
29.56
1.75
19.78
22.33
11.67
22.78

11
0
0
0.111
0.111
0
0.444

12
96.67
76.67
67.5
75.83
74.17
76.67

13
86.78
69.56
62.44
70.89
69.44
63.89

14
2.874
1.377
2.132
2.256
2.6
1.87

15
0.893
0.896
0.869
0.891
2.316
0.365

16
2.333
3.5
3
2.167
2.833
2

17
89
219.3
93
86
191.3
71.33

18
150.9
55.89
134
160.4
75.44
324.6

19
123.6
43.89
87.67
102.7
93.56
88

20
1.556
3
1.778
1.778
5.667
5.625

21
17
18.75
26.44
32.33
21.56
33.5

22
38.44
2
26.44
31.33
8.889
82

23
0
0
0
0
0
1.5

24
16.56
16.78
16.11
18.11
16.78
17.11

25
23
16
23.11
33
15.22
45.25

26
274.4
99.78
221.7
263.1
169
412.5

27
67.78
63.78
64.89
74.89
54
167.2

28
26
14.89
20.11
20.11
21.11
30.25

29
22.11
14.33
16
15
33.78
9

30
48.11
29.22
36.11
35.11
54.89
38.88

31
15.09
10.5
17.23
16.51
12.06
10.25

32
170.9
198.2
152.6
163.9
224.7
265

33
47.57
43.77
50.37
56.3
44
40.33

34
2.67
1.95
2.431
2.529
2.127
2.677

35
2.599
1.895
2.523
2.525
2.167
2.591

36
5.243
4.152
3.914
3.921
4.154
3.742

37
57
28.56
54.67
65.44
36.11
122.6

38
104.6
51.67
89.22
106.2
79.33
155.6

39
47.56
23.11
34.56
40.78
43.22
33

40
89

93
86

71.33

Table 133. Provided are the values of each of the parameters (as described above) measured in soybean accessions (line) under normal growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 134

Measured parameters in Soybean varieties (lines 7-12)

Line

Corr ID
Line-7
Line-8
Line-9
Line-10
Line-11
Line-12

1
73
72.33
68.67
73.67
68
70.67

2
66.5
65.67
62.33
67.67
61.67
64.33

3
8.456
8.089
8.256
7.733
8.156
7.889

4
48
52
44.17
52.67
56
47.5

5
2.667
2.5
1.833
3.5
3.333
1.5

6
41
38.33
31
39
27.33
32.67

7
11.67
12.11
8
9.111
6.778
10

8
4
4.333
2.111
1.889
3.444
1.222

9
21.33
17.67
20.33
16.11
28.11
16.56

10
11.11
28.22
24.11
36.44
39.67
32.33

11
0
0.556
0
3.889
0
0

12
101.7
98.33
75.83
116.7
76.67
71.67

13
89.78
82.11
70.56
101.7
79.56
67.22

14
1.98
2.712
2.777
2.754
3.7
2.836

15
3.9
0.783
1.183
1.984
1.033
0.832

16
3.5
2.5
2.167
2.333
2.167
2.167

17
88
75
80.67
75.67
76.33
77.33

18
46.88
176.2
143
105.4
184.3
187.3

19
80
126.6
115.1
159
178.7
131.3

20
2.875
3
1.25
2.667
1.778
3

21
8.5
22.78
21.75
10.67
23.78
25.67

22
9
42.11
32.75
25.67
45
44.33

23
0
0.333
0
1.111
0
0

24
18.78
18.89
16.78
21.11
19.33
20.78

25
8.25
25.44
21.88
16.33
22.56
24.22

26
136
302.8
260.5
264.4
363
318.7

27
45.44
83.22
64.33
52
76.89
67

28
4.125
20.11
17
9.222
28.11
22.56

29
9.033
16
15.89
14.56
30.44
18

30
14.25
36.11
32.75
23.78
58.56
40.56

31
7.297
11.38
15.68
10.83
12.98
15.16

32
160.7
196.3
155.3
178.1
204.4
164.2

33
34.23
44.27
53.67
42.47
43.6
52.2

34
2.12
2.581
2.583
2.668
2.62
2.578

35
2.218
2.487
2.474
2.713
2.512
2.609

36
4.803
4.357
4.203
4.825
4.116
3.827

37
20.38
68.22
55.75
40.11
70.56
73

38
61
119
103.3
98.44
141.8
123.1

39
36.44
50.78
43.63
58.33
71.22
50.11

40
88
75
80.67
75.67
76.33
77.33

Table 134. Provided are the values of each of the parameters (as described above) measured in soybean accessions (line) under normal growth conditions. Growth conditions are specified in the experimental procedure section.

TABLE 135

Correlation between the expression level of selected genes of some

embodiments of the invention in various tissues and the phenotypic

performance under normal conditions across soybean varieties

Gene

Exp.
Corr.
Gene

Exp.
Corr.

Name
R
P value
set
Set ID
Name
R
P value
set
Set ID

LAB800
0.74
1.34E−02
8
13
LAB800
0.72
1.88E−02
8
12

LAB800
0.82
1.37E−02
9
31
LAB800
0.78
2.59E−03
10
11

LAB801
0.78
7.42E−03
7
13
LAB801
0.80
5.32E−03
7
12

LAB801
0.77
8.64E−03
5
10
LAB801
0.72
1.79E−02
5
19

LAB801
0.82
1.23E−02
9
12
LAB801
0.75
4.70E−03
4
10

LAB804
0.84
8.63E−03
9
15
LAB804
0.79
2.40E−03
10
9

LAB807
0.72
1.82E−02
7
31
LAB807
0.72
1.83E−02
7
33

LAB807
0.82
3.44E−03
5
17
LAB807
0.84
8.99E−03
9
15

LAB807
0.76
2.94E−02
9
9
LAB809
0.83
8.97E−04
4
22

LAB809
0.83
7.81E−04
4
18
LAB809
0.83
8.05E−04
4
23

LAB809
0.89
9.50E−05
4
27
LAB809
0.84
6.37E−04
4
7

LAB809
0.85
5.19E−04
4
25
LAB810
0.71
2.02E−02
8
10

LAB810
0.75
3.18E−02
9
9
LAB810
0.73
4.10E−02
9
30

LAB810
0.76
2.94E−02
9
14
LAB810
0.91
1.95E−03
9
29

LAB810
0.73
7.61E−03
10
4
LAB810
0.78
2.87E−03
10
5

LAB811
0.78
7.90E−03
7
32
LAB811
0.73
1.64E−02
8
22

LAB811
0.72
1.84E−02
8
18
LAB811
0.76
1.06E−02
8
26

LAB801
0.73
7.28E−03
4
34
LAB802
0.74
3.75E−02
9
36

LAB804
0.74
1.44E−02
8
36
LAB807
0.77
3.21E−03
10
36

LAB809
0.83
9.26E−04
4
37
LAB810
0.71
4.86E−02
9
39

LAB810
0.71
9.69E−03
10
39
LAB811
0.71
2.17E−02
8
37

LAB811
0.79
6.78E−03
8
2
LAB811
0.79
6.66E−03
8
34

LAB811
0.72
1.77E−02
8
38
LAB802
0.83
1.08E−02
5
40

LAB800
0.82
1.25E−02
5
40
LAB803
0.88
3.86E−03
5
40

LAB801
0.90
2.27E−03
8
40
LAB805
0.81
1.38E−02
5
40

LAB802
0.74
5.68E−02
9
40
LAB809
0.77
2.44E−02
5
40

LAB804
0.93
7.37E−04
8
40
LAB800
0.71
2.07E−02
1
40

LAB807
0.76
2.70E−02
8
40

Table 135. Correlations (R) between the genes expression levels in various tissues and the phenotypic performance. “Corr. ID”—correlation set ID according to the correlated parameters Table above. “Exp. Set”—Expression set. “R” = Pearson correlation coefficient; “P” = p value.

Example 15
Plant Fiber Development in Cotton Production of Cotton Transcriptom and High Throughput Correlation Analysis Using Cotton Oligonucleotide Microarray

In order to conduct high throughput gene expression correlation analysis, the present inventors used cotton oligonucleotide microarray, designed and produced by “Comparative Evolutionary Genomics of Cotton” [Hypertext Transfer Protocol cottonevolution (dot) info/). This Cotton Oligonucleotide Microarray is composed of 12,006 Integrated DNA Technologies (IDT) oligonucleotides derived from an assembly of more than 180,000 Gossypium ESTs sequenced from 30 cDNA libraries. For additional details see PCT/IL2005/000627 and PCT/IL2007/001590 which are fully incorporated herein by reference.

TABLE 136

Cotton transcriptom experimental sets

Expression Set
Set ID

cotton fiber 5 d
1

cotton fiber 10 d
2

cotton fiber 15 d
3

Table 136. Provided are the cotton transcriptom expression sets. “5 d” = 5 days post anthesis; “10 d” = 10 days post anthesis; “15 d” = 15 days post anthesis. “DPA” = days-past-anthesis.

In order to define correlations between the levels of RNA expression and fiber length, fibers from 8 different cotton lines were analyzed. These fibers were selected showing very good fiber quality and high lint index (Pima types, originating from other cotton species, namely G. barbadense), different levels of quality and lint indexes from various G. hirsutum lines: good quality and high lint index (Acala type), and poor quality and short lint index (Tamcot type, and old varieties). A summary of the fiber length of the different lines is provided in Table 137.

Experimental Procedures

RNA extraction—Fiber development stages, representing different fiber characteristics, at 5, 10 and 15 DPA were sampled and RNA was extracted as described above.

Fiber length assessment—Fiber length of the selected cotton lines was measured using fibrograph. The fibrograph system was used to compute length in terms of “Upper Half Mean” length. The upper half mean (UHM) is the average length of longer half of the fiber distribution. The fibrograph measures length in span lengths at a given percentage point World Wide Web (dot) cottoninc (dot) com/ClassificationofCotton/?Pg=4#Length].

Experimental Results

Eight different cotton lines were grown, and their fiber length was measured. The fibers UHM values are summarized in Table 137 herein below. The R square was calculated (Table 138).

TABLE 137

Summary of the fiber length of the 8 different cotton lines

Line/Correlation ID
Fiber Length (UHM)

Line-1
1.21

Line-2
1.1

Line-3
1.36

Line-4
1.26

Line-5
0.89

Line-6
1.01

Line-7
1.06

Line-8
1.15

Table 137: Presented are the fiber length means of 8 different cotton lines.

TABLE 138

Correlation between the expression level of selected genes of some

embodiments of the invention in various tissues and the phenotypic

performance under normal conditions in cotton

Gene Name
R
P value
Exp. set

LAB672
0.84
8.50E−03
1

Table 138. Correlations (R) between the genes expression levels in various tissues and the phenotypic performance. “Corr. ID”—correlation set ID according to the correlated parameters Table above. “Exp. Set”—Expression set. “R” = Pearson correlation coefficient; “P” = p value

Example 16
Production of Arabidopsis Transcriptom and High Throughput Correlation Analysis of Yield, Biomass and/or Vigor Related Parameters Using 44K Arabidopsis Full Genome Oligonucleotide Micro-Array

To produce a high throughput correlation analysis comparing between plant phenotype and gene expression level, the present inventors utilized an Arabidopsis thaliana oligonucleotide micro-array, produced by Agilent Technologies [Hypertext Transfer Protocol://World Wide Web (dot) chem. (dot) agilent (dot) com/Scripts/PDS (dot) asp?1Page=50879]. The array oligonucleotide represents about 40,000 A. thaliana genes and transcripts designed based on data from the TIGR ATH1 v.5 database and Arabidopsis MPSS (University of Delaware) databases. To define correlations between the levels of RNA expression and yield, biomass components or vigor related parameters, various plant characteristics of 15 different Arabidopsis ecotypes were analyzed. Among them, nine ecotypes encompassing the observed variance were selected for RNA expression analysis. The correlation between the RNA levels and the characterized parameters was analyzed using Pearson correlation test [Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot) com/hyperstat/A34739 (dot) html].

Experimental Procedures

Analyzed Arabidopsis tissues—Five tissues at different developmental stages including root, leaf, flower at anthesis, seed at 5 days after flowering (DAF) and seed at 12 DAF, representing different plant characteristics, were sampled and RNA was extracted as described above. Each micro-array expression information tissue type has received a Set ID as summarized in Table 139 below.

TABLE 139

PTissues used for Arabidopsis transcriptom expression sets

Expression Set
Set ID

Leaf
1

Root
2

Seed 5 DAF
3

Flower
4

Seed 12 DAF
5

Table 139: Provided are the identification (ID) numbers of each of the Arabidopsis expression sets (1-5). DAF = days after flowering.

Yield components and vigor related parameters assessment—Nine Arabidopsis ecotypes were used in each of 5 repetitive blocks (named A, B, C, D and E), each containing 20 plants per plot. The plants were grown in a greenhouse at controlled conditions in 22° C., and the N:P:K fertilizer (20:20:20; weight ratios) [nitrogen (N), phosphorus (P) and potassium (K)] was added. During this time data was collected, documented and analyzed. Additional data was collected through the seedling stage of plants grown in vertical grown transparent agar plates (seedling analysis). Most of chosen parameters were analyzed by digital imaging.

Digital imaging for seedling analysis—A laboratory image acquisition system was used for capturing images of plantlets sawn in square agar plates. The image acquisition system consists of a digital reflex camera (Canon EOS 300D) attached to a 55 mm focal length lens (Canon EF-S series), mounted on a reproduction device (Kaiser RS), which included 4 light units (4×150 Watts light bulb) and located in a darkroom.

Digital imaging in Greenhouse—The image capturing process was repeated every 3-4 days starting at day 7 till day 30. The same camera attached to a 24 mm focal length lens (Canon EF series), placed in a custom made iron mount, was used for capturing images of larger plants sawn in white tubs in an environmental controlled greenhouse. The white tubs were square shape with measurements of 36×26.2 cm and 7.5 cm deep. During the capture process, the tubs were placed beneath the iron mount, while avoiding direct sun light and casting of shadows. This process was repeated every 3-4 days for up to 30 days.

An image analysis system was used, which consists of a personal desktop computer (Intel P43.0 GHz processor) and a public domain program—ImageJ 1.37, Java based image processing program, which was developed at the U.S. National Institutes of Health and is freely available on the internet at Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/. Images were captured in resolution of 6 Mega Pixels (3072×2048 pixels) and stored in a low compression JPEG (Joint Photographic Experts Group standard) format. Next, analyzed data was saved to text files and processed using the JMP statistical analysis software (SAS institute).

Leaf analysis—Using the digital analysis leaves data was calculated, including leaf number, area, perimeter, length and width. On day 30, 3-4 representative plants were chosen from each plot of blocks A, B and C. The plants were dissected, each leaf was separated and was introduced between two glass trays, a photo of each plant was taken and the various parameters (such as leaf total area, laminar length etc.) were calculated from the images. The blade circularity was calculated as laminar width divided by laminar length.

Root analysis—During 17 days, the different ecotypes were grown in transparent agar plates. The plates were photographed every 3 days starting at day 7 in the photography room and the roots development was documented (see examples in FIGS. 3A-F). The growth rate of roots was calculated according to Formula XVII.

Relative growth rate of root length=Regression coefficient of root length along time course (measured in cm per day). Formula XVII

Vegetative growth rate analysis—was calculated according to Formula XVII below.

Relative growth rate of vegetative growth=Regression coefficient of leaf area along time course (measured in cm²per day). Formula XVII:

The analysis was ended with the appearance of overlapping plants.

For comparison between ecotypes the calculated rate was normalized using plant developmental stage as represented by the number of true leaves. In cases where plants with 8 leaves had been sampled twice (for example at day 10 and day 13), only the largest sample was chosen and added to the Anova comparison.

Seeds in siliques analysis—On day 70, 15-17 siliques were collected from each plot in blocks D and E. The chosen siliques were light brown color but still intact. The siliques were opened in the photography room and the seeds were scatter on a glass tray, a high resolution digital picture was taken for each plot. Using the images the number of seeds per silique was determined.

Seeds average weight—At the end of the experiment all seeds from plots of blocks A-C were collected. An average weight of 0.02 grams was measured from each sample, the seeds were scattered on a glass tray and a picture was taken. Using the digital analysis, the number of seeds in each sample was calculated.

Oil percentage in seeds—At the end of the experiment all seeds from plots of blocks A-C were collected. Columbia seeds from 3 plots were mixed grounded and then mounted onto the extraction chamber. 210 ml of n-Hexane (Cat No. 080951 Biolab Ltd.) were used as the solvent. The extraction was performed for 30 hours at medium heat 50° C. Once the extraction has ended the n-Hexane was evaporated using the evaporator at 35° C. and vacuum conditions. The process was repeated twice. The information gained from the Soxhlet extractor (Soxhlet, F. Die gewichtsanalytische Bestimmung des Milchfettes, Polytechnisches J. (Dingler's) 1879, 232, 461) was used to create a calibration curve for the Low Resonance NMR. The content of oil of all seed samples was determined using the Low Resonance NMR (MARAN Ultra-Oxford Instrument) and its MultiQuant software package.

Silique length analysis—On day 50 from sowing, 30 siliques from different plants in each plot were sampled in block A. The chosen siliques were green-yellow in color and were collected from the bottom parts of a grown plant's stem. A digital photograph was taken to determine silique's length.

Dry weight and seed yield—On day 80 from sowing, the plants from blocks A-C were harvested and left to dry at 30° C. in a drying chamber. The biomass and seed weight of each plot was separated, measured and divided by the number of plants. Dry weight=total weight of the vegetative portion above ground (excluding roots) after drying at 30° C. in a drying chamber; Seed yield per plant=total seed weight per plant (gr.).

Oil yield—The oil yield was calculated using Formula XIX.

Seed Oil yield=Seed yield per plant(gr.)*Oil % in seed. Formula XIX

Harvest Index (seed)—The harvest index was calculated using Formula IV (described above).

Experimental Results

Nine different Arabidopsis ecotypes were grown and characterized for 18 parameters (named as vectors). Table 140 describes the Arabidopsis correlated parameters. The average for each of the measured parameter was calculated using the JMP software (Tables 141-142) and a subsequent correlation analysis was performed (Table 143). Results were then integrated to the database.

TABLE 140

Arabidopsis correlated parameters (vectors)

Correlated parameter with
Correlation ID

Blade circularity
1

Dry matter per plant (gr.)
2

Harvest Index
3

Lamina length (cm)
4

Lamina width (cm)
5

Leaf width/length
6

Oil % per seed (%)
7

Oil yield per plant (mg)
8

Seeds per Pod (num)
9

Silique length (cm)
10

Total leaf area per plant (cm2)
11

Vegetative growth rate (cm²/day) till 8 true leaves
12

Fresh weight per plant (gr.) at bolting stage
13

Relative root length growth (cm/day) day 13
14

Root length day 13 (cm)
15

Root length day 7 (cm)
16

1000 Seed weight (gr.)
17

Seed yield per plant (gr.)
18

Table 140. Provided are the Arabidopsis correlated parameters (correlation ID Nos. 1-18). Abbreviations: Cm = centimeter(s); gr. = gram(s); mg = milligram(s).

The characterized values are summarized in Tables 141 and 142 below.

TABLE 141

Measured parameters in Arabidopsis ecotypes

Corr. ID

Line
1
2
3
4
5
6
7
8
9

Line-1
0.51
0.64
0.53
2.77
1.38
0.35
34.42
118.63
45.44

Line-2
0.48
1.27
0.35
3.54
1.70
0.29
31.19
138.73
53.47

Line-3
0.45
1.05
0.56
3.27
1.46
0.32
38.05
224.06
58.47

Line-4
0.37
1.28
0.33
3.78
1.37
0.26
27.76
116.26
35.27

Line-5
0.50
1.69
0.37
3.69
1.83
0.36
35.49
218.27
48.56

Line-6
0.38
1.34
0.32
4.60
1.65
0.27
32.91
142.11
37.00

Line-7
0.39
0.81
0.45
3.88
1.51
0.30
31.56
114.15
39.38

Line-8
0.49
1.21
0.51
3.72
1.82
0.34
30.79
190.06
40.53

Line-9
0.41
1.35
0.41
4.15
1.67
0.31
34.02
187.62
25.53

Table 141: Provided are the values of each of the parameters (as described above) measured in arabidopsis accessions (line). Growth conditions are specified in the experimental procedure section.

TABLE 142

Additional measured parameters in Arabidopsis ecotypes

Corr. ID

Line
10
11
12
13
14
15
16
17
18

Line-1
1.06
46.86
0.31
1.51
0.63
4.42
0.94
0.0203
0.34

Line-2
1.26
109.89
0.38
3.61
0.66
8.53
1.76
0.0230
0.44

Line-3
1.31
58.36
0.48
1.94
1.18
5.62
0.70
0.0252
0.59

Line-4
1.47
56.80
0.47
2.08
1.09
4.83
0.73
0.0344
0.42

Line-5
1.24
114.66
0.43
3.56
0.91
5.96
0.99
0.0202
0.61

Line-6
1.09
110.82
0.64
4.34
0.77
6.37
1.16
0.0263
0.43

Line-7
1.18
88.49
0.43
3.47
0.61
5.65
1.28
0.0205
0.36

Line-8
1.18
121.79
0.38
3.48
0.70
7.06
1.41
0.0226
0.62

Line-9
1.00
93.04
0.47
3.71
0.78
7.04
1.25
0.0235
0.55

Table 142: Provided are the values of each of the parameters (as described above) measured in arabidopsis accessions (line). Growth conditions are specified in the experimental procedure section.

TABLE 143

Correlation between the expression level of selected LAB genes of some

embodiments of the invention in various tissues and the phenotypic performance

under normal conditions across Arabidopsis accessions

Gene

Exp.
Corr.
Gene

Exp.
Corr.

Name
R
P value
set
Set ID
Name
R
P value
set
Set ID

LAB618
0.81
1.51E−02
2
2
LAB618
0.75
3.37E−02
2
12

LAB618
0.80
3.24E−02
3
3
LAB618
0.73
4.11E−02
4
4

LAB618
0.83
1.16E−02
4
12
LAB618
0.79
1.98E−02
1
18

LAB618
0.79
1.94E−02
1
8
LAB619
0.71
7.36E−02
3
12

LAB619
0.85
7.36E−03
1
3
LAB619
0.87
5.40E−03
1
7

Table 143. Correlations (R) between the genes expression levels in various tissues and the phenotypic performance. “Corr. ID”—correlation set ID according to the correlated parameters Table above. “Exp. Set”—Expression set. “R” = Pearson correlation coefficient; “P” = p value

Example 17
Production of Arabidopsis Transcriptom and High Throughput Correlation Analysis Using 44K Arabidopsis Oligonucleotide Micro-Array

In order to produce a high throughput correlation analysis comparing between plant phenotype and gene expression level, the present inventors utilized a Arabidopsis oligonucleotide micro-array, produced by Agilent Technologies [Hypertext Transfer Protocol://World Wide Web (dot) chem (dot) agilent (dot) com/Scripts/PDS (dot) asp?1Page=50879]. The array oligonucleotide represents about 44,000 Arabidopsis genes and transcripts. To define correlations between the levels of RNA expression with NUE, yield components or vigor related parameters various plant characteristics of 14 different Arabidopsis ecotypes were analyzed. Among them, ten ecotypes encompassing the observed variance were selected for RNA expression analysis. The correlation between the RNA levels and the characterized parameters was analyzed using Pearson correlation test [Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot) com/hyperstat/A34739 (dot) html].

Experimental Procedures

Analyzed Arabidopsis tissues—Two tissues of plants [leaves and stems] growing at two different nitrogen fertilization levels (1.5 mM Nitrogen or 6 mM Nitrogen) were sampled and RNA was extracted as described above. Each micro-array expression information tissue type has received a Set ID as summarized Table 135 below.

TABLE 144

Arabidopsis transcriptom experimental sets

Expression Set
Set ID

Leaves at 6 mM Nitrogen fertilization
1

Leaves at 1.5 mM Nitrogen fertilization
2

Stems at 1.5 mM Nitrogen fertilization
3

Stem at 6 mM Nitrogen fertilization
4

Table 144. Provided are the arabidopsis transcriptome expression sets.

Arabidopsis yield components and vigor related parameters under different nitrogen fertilization levels assessment—10 Arabidopsis accessions in 2 repetitive plots each containing 8 plants per plot were grown at greenhouse. The growing protocol used was as follows: surface sterilized seeds were sown in Eppendorf tubes containing 0.5×Murashige-Skoog basal salt medium and grown at 23° C. under 12-hour light and 12-hour dark daily cycles for 10 days. Then, seedlings of similar size were carefully transferred to pots filled with a mix of perlite and peat in a 1:1 ratio. Constant nitrogen limiting conditions were achieved by irrigating the plants with a solution containing 1.5 mM inorganic nitrogen in the form of KNO₃, supplemented with 2 mM CaCl₂, 1.25 mM KH₂PO₄, 1.50 mM MgSO₄, 5 mM KCl, 0.01 mM H₃B0₃and microelements, while normal irrigation conditions was achieved by applying a solution of 6 mM inorganic nitrogen also in the form of KNO₃, supplemented with 2 mM CaCl₂, 1.25 mM KH₂PO₄, 1.50 mM MgSO₄, 0.01 mM H₃B0₃and microelements. To follow plant growth, trays were photographed the day nitrogen limiting conditions were initiated and subsequently every 3 days for about 15 additional days. Rosette plant area was then determined from the digital pictures. ImageJ software was used for quantifying the plant size from the digital pictures [Hypertext Transfer Protocol://rsb (dot) info (dot) nih (dot) gov/ij/] utilizing proprietary scripts designed to analyze the size of rosette area from individual plants as a function of time. The image analysis system included a personal desktop computer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ 1.37 (Java based image processing program, which was developed at the U.S. National Institutes of Health and freely available on the internet [Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/]). Next, analyzed data was saved to text files and processed using the JMP statistical analysis software (SAS institute).

Data parameters collected are summarized in Table 145, hereinbelow.

TABLE 145

Arabidopsis correlated parameters (vectors)

Correlated parameter with
Correlation ID

N 1.5 mM 1000 Seeds weight (gr)
1

N 1.5 mM Biomass reduction compared to 6 mM
2

N 1.5 mM DW/SPAD (gr/SPAD unit)
3

N 1.5 mM Dry Weight (gr)
4

N 1.5 mM Harvest Index
5

N 1.5 mM Leaf Blade Area 10 day (cm²)
6

N 1.5 mM Leaf Number 10 day (num)
7

N 1.5 mM RGR of Rosette Area 3 day (cm²/day)
8

N 1.5 mM Rosette Area 10 day (cm²)
9

N 1.5 mM Rosette Area 8 day (cm²)
10

N 1.5 mM SPAD/DW (SPAD unit/gr. Plant)
11

N 1.5 mM Seed Yield (gr/plant)
12

N 1.5 mM Seed yield reduction compared to 6 mM
13

N 1.5 mM Spad/FW (SPAD unit/gr)
14

N 1.5 mM seed yield/spad (gr/SPAD unit)
15

N 1.5 mM seed yield per leaf blade (gr/cm²)
16

N 1.5 mM seed yield per rosette area day 10 (gr/cm²)
17

N 1.5 mM t50 Flowering (days)
18

N 6 mM DW/SPAD (gr/SPAD unit)
19

N 6 mM Spad/FW (SPAD unit/gr)
20

N 6 mM 1000 Seeds weight (gr)
21

N 6 mM Dry Weight (gr)
22

N 6 mM Harvest Index
23

N 6 mM Leaf Blade Area 10 day (cm²)
24

N 6 mM Leaf Number 10 day (num)
25

N 6 mM RGR of Rosette Area 3 day (cm²/day)
26

N 6 mM Rosette Area 10 day (cm²)
27

N 6 mM Rosette Area 8 day (cm²)
28

N 6 mM Seed Yield (gr/plant)
29

N 6 mM Seed yield/N unit (gr./SPAD unit)
30

N 6 mM seed yield/rosette area day 10 day (gr/cm²)
31

N 6 mM seed yield/leaf blade (gr/cm²)
32

N 6 mM spad/DW (gN/g plant) (SPAD unit/gr)
33

N 6 mM t50 Flowering (days)
34

Table 145. “N” = Nitrogen at the noted concentrations; “gr.” = grams; “SPAD” = chlorophyll levels; “t50” = time where 50% of plants flowered; “gr./SPAD unit” = plant biomass expressed in grams per unit of nitrogen in plant measured by SPAD. “DW” = plant dry weight; “N level/DW” = plant Nitrogen level measured in SPAD unit per plant biomass [gr.]; “DW/N level” = plant biomass per plant [gr.]/SPAD unit;

Assessment of NUE, yield components and vigor-related parameters—Ten Arabidopsis ecotypes were grown in trays, each containing 8 plants per plot, in a greenhouse with controlled temperature conditions for about 12 weeks. Plants were irrigated with different nitrogen concentration as described above depending on the treatment applied. During this time, data was collected documented and analyzed. Most of chosen parameters were analyzed by digital imaging.

Digital imaging—Greenhouse assay

An image acquisition system, which consists of a digital reflex camera (Canon EOS 400D) attached with a 55 mm focal length lens (Canon EF-S series) placed in a custom made Aluminum mount, was used for capturing images of plants planted in containers within an environmental controlled greenhouse. The image capturing process is repeated every 2-3 days starting at day 9-12 till day 16-19 (respectively) from transplanting.

An image processing system was used, which consists of a personal desktop computer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ 1.37, Java based image processing software, which was developed at the U.S. National Institutes of Health and is freely available on the internet at Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/. Images were captured in resolution of 10 Mega Pixels (3888×2592 pixels) and stored in a low compression JPEG (Joint Photographic Experts Group standard) format. Next, image processing output data was saved to text files and analyzed using the JMP statistical analysis software (SAS institute).

Leaf analysis—Using the digital analysis leaves data was calculated, including leaf number, leaf blade area, Rosette diameter and area.

Vegetative growth rate: the relative growth rate (RGR) of leaf blade area (Formula XX), leaf number (Formula XXI), rosette area (Formula XXII), rosette diameter (Formula XXIII), plot coverage (Formula XXIV) and Petiole Relative Area (XXV) are calculated as follows:

Relative growth rate of leaf blade area=Regression coefficient of leaf area along time course (measured in cm²per day). Formula XX

Relative growth rate of leaf number=Regression coefficient of leaf number along time course (measured in number per day). Formula XXI

Relative growth rate of rosette area=Regression coefficient of rosette area along time course (measured in cm²per day). Formula XXII

Relative growth rate of rosette diameter=Regression coefficient of rosette diameter along time course (measured in cm per day). Formula XXIII

Relative growth rate of plot coverage=Regression coefficient of plot (measured in cm²per day). Formula XXIV

Petiole Relative Area=(Petiol area)/Rosette area (measured in %). Formula XXV:

Seed yield and 1000 seeds weight—At the end of the experiment all seeds from all plots were collected and weighed in order to measure seed yield per plant in terms of total seed weight per plant (gr.). For the calculation of 1000 seed weight, an average weight of 0.02 grams was measured from each sample, the seeds were scattered on a glass tray and a picture was taken. Using the digital analysis, the number of seeds in each sample was calculated.

Dry weight and seed yield—At the end of the experiment, plant were harvested and left to dry at 30° C. in a drying chamber. The biomass was separated from the seeds, weighed and divided by the number of plants. Dry weight=total weight of the vegetative portion above ground (excluding roots) after drying at 30° C. in a drying chamber.

Harvest Index—The harvest index was calculated using Formula IV as described above.

T₅₀days to flowering—Each of the repeats was monitored for flowering date.

Days of flowering was calculated from sowing date till 50% of the plots flowered.

Plant nitrogen level—The chlorophyll content of leaves is a good indicator of the nitrogen plant status since the degree of leaf greenness is highly correlated to this parameter. Chlorophyll content was determined using a Minolta SPAD 502 chlorophyll meter and measurement was performed at time of flowering. SPAD meter readings were done on young fully developed leaf. Three measurements per leaf were taken per plot. Based on this measurement, parameters such as the ratio between seed yield per nitrogen unit [seed yield/N level=seed yield per plant [gr.]/SPAD unit], plant DW per nitrogen unit [DW/N level=plant biomass per plant [gr.]/SPAD unit], and nitrogen level per gram of biomass [N level/DW=SPAD unit/plant biomass per plant (gr.)] were calculated.

Percent of seed yield reduction—measures the amount of seeds obtained in plants when grown under nitrogen-limiting conditions compared to seed yield produced at normal nitrogen levels expressed in %.

Experimental Results

10 different Arabidopsis accessions (ecotypes) were grown and characterized for 37 parameters as described above (Table 145). The average for each of the measured parameters was calculated using the JMP software and values are summarized in Table 146-149 below. Subsequent correlation analysis between the various transcriptom sets (Table 144) and the measured parameters was conducted. Following are the results integrated to the database.

TABLE 146

Measured parameters in Arabidopsis accessions

Corr. ID

Line
1
2
3
4
5
6
7
8

Line-1
0.016
60.75
0.0060
0.16
0.19
0.33
6.88
0.63

Line-2
0.016
76.71

0.12
0.20
0.27
7.31
0.79

Line-3
0.018
78.56

0.08
0.29
0.37
7.31
0.50

Line-4
0.014
78.14
0.0041
0.11
0.08
0.39
7.88
0.49

Line-5
0.022
78.64

0.12
0.07
0.37
7.75
0.72

Line-6
0.015
73.19
0.0051
0.13
0.24
0.39
7.63
0.83

Line-7
0.014
83.07

0.11
0.18
0.35
7.19
0.65

Line-8
0.022
77.19

0.15
0.08
0.38
8.63
0.67

Line-9
0.019
70.12
0.0059
0.17
0.08
0.31
5.93
0.64

Line-10
0.018
62.97
0.0063
0.18
0.03
0.37
7.94
0.61

Table 146. Provided are the measured parameters under various treatments in various ecotypes (Arabidopsis accessions). Growth conditions are specified in the experimental procedure section.

TABLE 147

Additional Measured parameters in Arabidopsis accessions

Corr. ID

Line
9
10
11
12
13
14
15
16

Line-1
1.43
0.76
167.30
0.03
72.56
45.59
0.0012
0.09

Line-2
1.33
0.71

0.03
84.70

0.09

Line-3
1.77
1.06

0.02
78.78

0.06

Line-4
1.97
1.16
241.06
0.01
88.00
42.11
0.0004
0.03

Line-5
1.83
1.00

0.01
92.62

0.02

Line-6
1.82
0.91
194.98
0.03
76.71
53.11
0.0012
0.08

Line-7
1.64
0.94

0.02
81.94

0.06

Line-8
2.00
1.12

0.01
91.30

0.03

Line-9
1.15
0.64
169.34
0.01
85.76
67.00
0.0005
0.04

Line-10
1.75
1.00
157.82
0.01
91.82
28.15
0.0002
0.01

Table 147. Provided are the measured parameters under various treatments in various ecotypes (Arabidopsis accessions). Growth conditions are specified in the experimental procedure section.

TABLE 148

Additional Measured parameters in Arabidopsis accessions

Corr. ID

Line
17
18
19
20
21
22
23
24
25

Line-1
0.0221
15.97
0.02
22.49
0.0147
0.42
0.28
0.34
6.25

Line-2
0.0190
20.97

0.0169
0.53
0.31
0.31
7.31

Line-3
0.0136
14.84

0.0178
0.38
0.28
0.52
8.06

Line-4
0.0052
24.71
0.02
28.27
0.0121
0.52
0.16
0.45
8.75

Line-5
0.0050
23.70

0.0155
0.58
0.21
0.43
8.75

Line-6
0.0178
18.06
0.02
33.32
0.0154
0.50
0.28
0.50
8.38

Line-7
0.0127
19.49

0.0140
0.63
0.17
0.43
7.13

Line-8
0.0068
23.57

0.0166
0.65
0.21
0.51
9.44

Line-9
0.0118
21.89
0.01
39.00
0.0161
0.57
0.17
0.41
6.31

Line-10
0.0032
23.57
0.03
17.64
0.0160
0.50
0.14
0.43
8.06

Table 148. Provided are the measured parameters under various treatments in various ecotypes (Arabidopsis accessions). Growth conditions are specified in the experimental procedure section.

TABLE 149

Additional Measured parameters in Arabidopsis accessions

Corr. ID

Line
26
27
28
29
30
31
32
33
34

Line-1
0.69
1.41
0.76
0.12
0.0042
0.0824
0.34
53.71
16.37

Line-2
1.02
1.57
0.86
0.17

0.1058
0.53

20.50

Line-3
0.61
2.67
1.48
0.11

0.0405
0.21

14.63

Line-4
0.60
2.42
1.28
0.08
0.0030
0.0339
0.18
54.62
24.00

Line-5
0.65
2.14
1.10
0.12

0.0556
0.28

23.60

Line-6
0.68
2.47
1.24
0.14
0.0053
0.0570
0.28
66.48
15.03

Line-7
0.58
1.97
1.09
0.11

0.0554
0.25

19.75

Line-8
0.61
2.72
1.41
0.14

0.0507
0.27

22.89

Line-9
0.52
1.64
0.89
0.09
0.0033
0.0582
0.24
68.05
18.80

Line-10
0.48
2.21
1.22
0.07
0.0023
0.0307
0.16
35.55
23.38

Table 149. Provided are the measured parameters under various treatments in various ecotypes (Arabidopsis accessions). Growth conditions are specified in the experimental procedure section.

TABLE 150

Correlation between the expression level of selected LAB genes of some embodiments

of the invention in various tissues and the phenotypic performance under low

nitrogen and normal conditions across Arabidopsis accessions

Gene

Exp.
Corr.
Gene

Exp.
Corr.

Name
R
P value
set
Set ID
Name
R
P value
set
Set ID

LAB617
0.72
1.78E−02
2
1
LAB617
0.71
2.06E−02
1
4

LAB617
0.73
1.76E−02
1
34
LAB617
0.83
3.17E−03
3
1

Table 150. Correlations (R) between the genes expression levels in various tissues and the phenotypic performance. “Corr. ID”—correlation set ID according to the correlated parameters Table above. “Exp. Set”—Expression set. “R” = Pearson correlation coefficient; “P” = p value

Example 18
Identification of Genes which Increase Abst, Growth Rate, Vigor, Yield, Biomass, Oil Content, Wue, Nue and/or Fue in Plants

Based on the above described bioinformatics and experimental tools, the present inventors have identified 218 genes which exhibit a major impact on abiotic stress tolerance, plant yield, oil content, growth rate, vigor, biomass, fiber yield and quality, photosynthetic capacity, growth rate, nitrogen use efficiency, water use efficiency and fertilizer use efficiency when expression thereof is increased in plants. The identified genes, their curated polynucleotide and polypeptide sequences, as well as their updated sequences according to GenBank database are summarized in Table 151, hereinbelow.

TABLE 151

Identified genes for increasing abiotic stress tolerance, water use

efficiency, yield, growth rate, vigor, biomass, growth rate, oil content,

fiber yield, fiber quality, nitrogen use efficiency and fertilizer use

efficiency of a plant

Polyn.
Polyp.

SEQ ID
SEQ ID

Gene Name
Cluster tag
NO:
NO:

LAB616

arabidopsis|10v1|AT1G78230
1
475

LAB617

arabidopsis|10v1|AT3G62580
2
476

LAB618

arabidopsis|10v1|AT4G21190
3
477

LAB619

arabidopsis|10v1|AT4G39970
4
478

LAB620
barley|10v2|AV835060
5
479

LAB621
barley|10v2|BE060696
6
480

LAB622
barley|10v2|BE194055
7
481

LAB623
barley|10v2|BE194908
8
482

LAB624
barley|10v2|BE413570
9
483

LAB625
barley|10v2|BE454533
10
484

LAB626
barley|10v2|BE455595
11
485

LAB627
barley|10v2|BF254362
12
486

LAB628
barley|10v2|BF262375
13
487

LAB629
barley|10v2|BF619383
14
488

LAB630
barley|10v2|BF621759
15
489

LAB631
barley|10v2|BF622249
16
490

LAB632
barley|10v2|BF622714
17
491

LAB633
barley|10v2|BF624407
18
492

LAB634
barley|10v2|BF625108
19
493

LAB635
barley|10v2|BF625296
20
494

LAB636
barley|10v2|BF626425
21
495

LAB637
barley|10v2|BG299942
22
496

LAB638
barley|10v2|BI952365
23
497

LAB639
barley|10v2|BI955769
24
498

LAB640
barley|10v2|BI960586
25
499

LAB641
barley|10v2|BJ463886
26
500

LAB642
barley|10v2|BM370254
27
501

LAB644
barley|10v2|BQ663993
28
502

LAB645
barley|10v2|BU990670
29
503

LAB647
barley|10v2|GH213121
30
504

LAB649
canola|11v1|EE410383
31
505

LAB650
cotton|11v1|BE053207
32
506

LAB651
foxtail_millet|11v3|EC612491
33
507

LAB652
foxtail_millet|11v3|EC613059
34
508

LAB653
foxtail_millet|11v3|EC613770
35
509

LAB654
foxtail_millet|11v3|
36
510

PHY7SI009391M

LAB655
foxtail_millet|11v3|
37
511

PHY7SI009629M

LAB656
foxtail_millet|11v3|
38
512

PHY7SI009779M

LAB657
foxtail_millet|11v3|
39
513

PHY7SI011238M

LAB659
foxtail_millet|11v3|
40
514

PHY7SI014411M

LAB660
foxtail_millet|11v3|
41
515

PHY7SI017763M

LAB661
foxtail_millet|11v3|
42
516

PHY7SI021724M

LAB662
foxtail_millet|11v3|
43
517

PHY7SI022125M

LAB663
foxtail_millet|11v3|
44
518

PHY7SI022834M

LAB664
foxtail_millet|11v3|
45
519

PHY7SI022902M

LAB665
foxtail_millet|11v3|
46
520

PHY7SI023281M

LAB666
foxtail_millet|11v3|
47
521

PHY7SI023422M

LAB667
foxtail_millet|11v3|
48
522

PHY7SI029211M

LAB668
foxtail_millet|11v3|
49
523

PHY7SI030817M

LAB669
foxtail_millet|11v3|
50
524

PHY7SI034438M

LAB670
foxtail_millet|11v3|
51
525

PHY7SI038207M

LAB671
foxtail_millet|11v3|
52
526

PHY7SI040241M

LAB672
gossypium_raimondii|12v1|
53
527

DR458820

LAB673
maize|10v1|AA979901
54
528

LAB675
maize|10v1|AF143681
55
529

LAB676
maize|10v1|AI372115
56
530

LAB677
maize|10v1|AI372353
57
531

LAB678
maize|10v1|AI619182
58
532

LAB679
maize|10v1|AI622384
59
533

LAB680
maize|10v1|AI622752
60
534

LAB681
maize|10v1|AI637066
61
535

LAB682
maize|10v1|AI677194
62
536

LAB683
maize|10v1|AI691657
63
537

LAB684
maize|10v1|AI734652
64
538

LAB685
maize|10v1|AI737066
65
539

LAB686
maize|10v1|AI737444
66
540

LAB687
maize|10v1|AI740094
67
541

LAB688
maize|10v1|AI795604
68
542

LAB689
maize|10v1|AI795742
69
543

LAB690
maize|10v1|AI795751
70
544

LAB691
maize|10v1|AI857184
71
545

LAB692
maize|10v1|AI861347
72
546

LAB693
maize|10v1|AI901930
73
547

LAB694
maize|10v1|AI902032
74
548

LAB695
maize|10v1|AI932122
75
549

LAB696
maize|10v1|AI947867
76
550

LAB697
maize|10v1|AI947885
77
551

LAB698
maize|10v1|AJ006547
78
552

LAB700
maize|10v1|AW066791
79
553

LAB701
maize|10v1|AW231459
80
554

LAB702
maize|10v1|AW330591
81
555

LAB703
maize|10v1|AW355959
82
556

LAB704
maize|10v1|AW461004
83
557

LAB705
maize|10v1|AW498362
84
558

LAB706
maize|10v1|AW499427
85
559

LAB707
maize|10v1|AW787305
86
560

LAB708
maize|10v1|AW787498
87
561

LAB709
maize|10v1|BE454007
88
562

LAB710
maize|10v1|BM378479
89
563

LAB711
maize|10v1|CD988313
90
564

LAB712
maize|10v1|CV985191
91
565

LAB713
maize|10v1|DN223227
92
566

LAB714
maize|10v1|T18853
93
567

LAB716
poplar|10v1|BU817192
94
568

LAB717
poplar|10v1|BU869216
95
569

LAB718
poplar|10v1|CA822891
96
570

LAB719
rice|11v1|AU062544
97
571

LAB720
rice|11v1|AU162158
98
572

LAB721
rice|11v1|BE228839
99
573

LAB722
rice|11v1|BI305463
100
574

LAB723
rice|11v1|BI306005
101
575

LAB724
rice|11v1|BI796493
102
576

LAB725
rice|11v1|BI805675
103
577

LAB726
rice|11v1|BI805831
104
578

LAB727
rice|11v1|BI806975
105
579

LAB728
rice|11v1|BI807671
106
580

LAB729
rice|11v1|BM420747
107
581

LAB730
rice|11v1|C72652
108
582

LAB731
rice|11v1|CA766103
109
583

LAB732
rice|11v1|CB210565
110
584

LAB733
rice|11v1|CB210910
111
585

LAB734
rice|11v1|CI305545
112
586

LAB735
rice|11v1|CK040817
113
587

LAB736
rice|gb170|OS01G70010
114
588

LAB738
rice|gb170|OS05G30750
115
589

LAB739
rice|gb170|OS05G46500
116
590

LAB740

sorghum|11v1|SB03G000850
117
591

LAB741

sorghum|11v1|SB03G007380
118
592

LAB742

sorghum|11v1|SB09G004130
119
593

LAB744

sorghum|12v1|AW286835
120
594

LAB746

sorghum|12v1|CF759842
121
595

LAB748

sorghum|12v1|SB01G002840
122
596

LAB749

sorghum|12v1|SB01G008190
123
597

LAB750

sorghum|12v1|SB01G025570
124
598

LAB751

sorghum|12v1|SB01G031145
125
599

LAB752

sorghum|12v1|SB01G035760
126
600

LAB753

sorghum|12v1|SB01G040510
127
601

LAB754

sorghum|12v1|SB01G049100
128
602

LAB755

sorghum|12v1|SB02G003700
129
603

LAB756

sorghum|12v1|SB02G006790
130
604

LAB757

sorghum|12v1|SB02G028150
131
605

LAB758

sorghum|12v1|SB02G034770
132
606

LAB759

sorghum|12v1|SB02G039700
133
607

LAB760

sorghum|12v1|SB02G040060
134
608

LAB761

sorghum|12v1|SB03G005990
135
609

LAB762

sorghum|12v1|SB03G027180
136
610

LAB763

sorghum|12v1|SB03G030230
137
611

LAB764

sorghum|12v1|SB03G034230
138
612

LAB765

sorghum|12v1|SB03G034960
139
613

LAB767

sorghum|12v1|SB03G035060
140
614

LAB768

sorghum|12v1|SB03G038630
141
615

LAB769

sorghum|12v1|SB03G043250
142
616

LAB770

sorghum|12v1|SB04G004050
143
617

LAB771

sorghum|12v1|SB04G025650
144
618

LAB772

sorghum|12v1|SB04G027690
145
619

LAB773

sorghum|12v1|SB04G028210
146
620

LAB774

sorghum|12v1|SB05G005150
147
621

LAB775

sorghum|12v1|SB06G002090
148
622

LAB776

sorghum|12v1|SB06G017150
149
623

LAB777

sorghum|12v1|SB06G018340
150
624

LAB778

sorghum|12v1|SB06G018520
151
625

LAB779

sorghum|12v1|SB06G018670
152
626

LAB780

sorghum|12v1|SB06G019310
153
627

LAB781

sorghum|12v1|SB06G019625
154
628

LAB782

sorghum|12v1|SB06G021340
155
629

LAB783

sorghum|12v1|SB06G022690
156
630

LAB784

sorghum|12v1|SB06G026000
157
631

LAB785

sorghum|12v1|SB06G026210
158
632

LAB786

sorghum|12v1|SB06G029200
159
633

LAB787

sorghum|12v1|SB06G032650
160
634

LAB788

sorghum|12v1|SB07G024190
161
635

LAB789

sorghum|12v1|SB08G004890
162
636

LAB790

sorghum|12v1|SB08G006240
163
637

LAB791

sorghum|12v1|SB08G016450
164
638

LAB792

sorghum|12v1|SB09G002040
165
639

LAB793

sorghum|12v1|SB09G002180
166
640

LAB794

sorghum|12v1|SB09G004650
167
641

LAB795

sorghum|12v1|SB09G022090
168
642

LAB796

sorghum|12v1|SB09G022210
169
643

LAB797

sorghum|12v1|SB10G000500
170
644

LAB798

sorghum|12v1|SB10G029140
171
645

LAB799

sorghum|12v1|SB10G029750
172
646

LAB800
soybean|11v1|GLYMA04G05720
173
647

LAB801
soybean|11v1|GLYMA04G12170
174
648

LAB802
soybean|11v1|GLYMA07G00450
175
649

LAB803
soybean|11v1|GLYMA08G19920
176
650

LAB804
soybean|11v1|GLYMA10G01750
177
651

LAB805
soybean|11v1|GLYMA10G06620
178
652

LAB807
soybean|11v1|GLYMA10G38880
179
653

LAB809
soybean|11v1|GLYMA20G26610
180
654

LAB810
soybean|11v1|GLYMA20G34960
181
655

LAB811
soybean|11v1|GLYMA20G36400
182
656

LAB813
sunflower|10v1|CD852214
183
657

LAB814
sunflower|10v1|DY905846
184
658

LAB815
sunflower|10v1|DY920340
185
659

LAB816
sunflower|10v1|DY933583
186
660

LAB817
sunflower|12v1|CD849392
187
661

LAB820
sunflower|12v1|DY913292
188
662

LAB821
sunflower|12v1|DY915017
189
663

LAB823
sunflower|12v1|DY937266
190
664

LAB824
tomato|11v1|AI773686
191
665

LAB825
tomato|11v1|AI778113
192
666

LAB827
tomato|11v1|AW035009
193
667

LAB829
tomato|11v1|AW217661
194
668

LAB830
tomato|11v1|AW615872
195
669

LAB831
tomato|11v1|BG125080
196
670

LAB832
tomato|11v1|BG125441
197
671

LAB833
tomato|11v1|BG126165
198
672

LAB834
tomato|11v1|BG128255
199
673

LAB835
tomato|11v1|BG128279
200
674

LAB836
tomato|11v1|BG132326
201
675

LAB837
tomato|11v1|BG626094
202
676

LAB839
tomato|11v1|BG629779
203
677

LAB840
tomato|11v1|BG629817
204
678

LAB841
tomato|11v1|BG734916
205
679

LAB842
tomato|11v1|BQ512667
206
680

LAB843
tomato|11v1|CO751611
207
681

LAB844
wheat|10v2|AF079526
208
682

LAB845
wheat|10v2|BE405715
209
683

LAB847
wheat|10v2|BE637663
210
684

LAB848
wheat|10v2|BI479093
211
685

LAB849
wheat|10v2|BQ166387
212
686

LAB850
wheat|10v2|CK211432
213
687

LAB669_H7
rice|11v1|BE229317
214
688

LAB744_H1
maize|10v1|AI665228
215
689

LAB756_H3
rice|11v1|BU572354
216
690

LAB745

sorghum|12v1|CD462918
217
—

LAB747

sorghum|12v1|EVOER1621
218
—

LAB616

arabidopsis|10v1|AT1G78230
219
475

LAB621
barley|10v2|BE060696
220
691

LAB624
barley|10v2|BE413570
221
692

LAB627
barley|10v2|BF254362
222
693

LAB640
barley|10v2|BI960586
223
499

LAB641
barley|10v2|BJ463886
224
694

LAB644
barley|10v2|BQ663993
225
695

LAB645
barley|10v2|BU990670
226
696

LAB647
barley|10v2|GH213121
227
697

LAB654
foxtail_millet|11v3|
228
510

PHY7SI009391M

LAB656
foxtail_millet|11v3|
229
698

PHY7SI009779M

LAB664
foxtail_millet|11v3|
230
519

PHY7SI022902M

LAB673
maize|10v1|AA979901
231
528

LAB676
maize|10v1|AI372115
232
699

LAB682
maize|10v1|AI677194
233
536

LAB710
maize|10v1|BM378479
234
563

LAB711
maize|10v1|CD988313
235
700

LAB726
rice|11v1|BI805831
236
578

LAB727
rice|11v1|BI806975
237
579

LAB731
rice|11v1|CA766103
238
583

LAB736
rice|gb170|OS01G70010
239
701

LAB749

sorghum|12v1|SB01G008190
240
597

LAB754

sorghum|12v1|SB01G049100
241
702

LAB756

sorghum|12v1|SB02G006790
242
604

LAB757

sorghum|12v1|SB02G028150
243
605

LAB763

sorghum|12v1|SB03G030230
244
611

LAB767

sorghum|12v1|SB03G035060
245
614

LAB770

sorghum|12v1|SB04G004050
246
703

LAB773

sorghum|12v1|SB04G028210
247
704

LAB774

sorghum|12v1|SB05G005150
248
621

LAB775

sorghum|12v1|SB06G002090
249
622

LAB777

sorghum|12v1|SB06G018340
250
624

LAB784

sorghum|12v1|SB06G026000
251
631

LAB787

sorghum|12v1|SB06G032650
252
705

LAB789

sorghum|12v1|SB08G004890
253
636

LAB797

sorghum|12v1|SB10G000500
254
706

LAB821
sunflower|12v1|DY915017
255
707

LAB832
tomato|11v1|BG125441
256
708

LAB843
tomato|11v1|CO751611
257
709

LAB844
wheat|10v2|AF079526
258
682

LAB848
wheat|10v2|BI479093
259
710

LAB850
wheat|10v2|CK211432
260
711

LAB745

sorghum|12v1|CD462918
261
—

LAB747

sorghum|12v1|EVOER1621
262
—

LAB616

arabidopsis|10v1|AT1G78230
263
475

LAB617

arabidopsis|10v1|AT3G62580
264
476

LAB618

arabidopsis|10v1|AT4G21190
265
477

LAB619

arabidopsis|10v1|AT4G39970
266
712

LAB620
barley|10v2|AV835060
267
479

LAB622
barley|10v2|BE194055
268
713

LAB623
barley|10v2|BE194908
269
482

LAB624
barley|10v2|BE413570
270
483

LAB625
barley|10v2|BE454533
271
484

LAB626
barley|10v2|BE455595
272
485

LAB627
barley|10v2|BF254362
273
714

LAB628
barley|10v2|BF262375
274
487

LAB629
barley|10v2|BF619383
275
488

LAB630
barley|10v2|BF621759
276
489

LAB631
barley|10v2|BF622249
277
490

LAB632
barley|10v2|BF622714
278
491

LAB633
barley|10v2|BF624407
279
492

LAB634
barley|10v2|BF625108
280
493

LAB635
barley|10v2|BF625296
281
715

LAB636
barley|10v2|BF626425
282
495

LAB637
barley|10v2|BG299942
283
496

LAB638
barley|10v2|BI952365
284
497

LAB639
barley|10v2|BI955769
285
716

LAB640
barley|10v2|BI960586
286
499

LAB641
barley|10v2|BJ463886
287
500

LAB642
barley|10v2|BM370254
288
717

LAB645
barley|10v2|BU990670
289
718

LAB647
barley|10v2|GH213121
290
504

LAB649
canola|11v1|EE410383
291
505

LAB650
cotton|11v1|BE053207
292
719

LAB651
foxtail_millet|11v3|EC612491
293
720

LAB652
foxtail_millet|11v3|EC613059
294
508

LAB653
foxtail_millet|11v3|EC613770
295
509

LAB654
foxtail_millet|11v3|
296
510

PHY7SI009391M

LAB655
foxtail_millet|11v3|
297
511

PHY7SI009629M

LAB656
foxtail_millet|11v3|
298
721

PHY7SI009779M

LAB657
foxtail_millet|11v3|
299
513

PHY7SI011238M

LAB659
foxtail_millet|11v3|
300
514

PHY7SI014411M

LAB660
foxtail_millet|11v3|
301
515

PHY7SI017763M

LAB661
foxtail_millet|11v3|
302
516

PHY7SI021724M

LAB662
foxtail_millet|11v3|
303
517

PHY7SI022125M

LAB663
foxtail_millet|11v3|
304
518

PHY7SI022834M

LAB664
foxtail_millet|11v3|
305
519

PHY7SI022902M

LAB665
foxtail_millet|11v3|
306
520

PHY7SI023281M

LAB666
foxtail_millet|11v3|
307
521

PHY7SI023422M

LAB667
foxtail_millet|11v3|
308
522

PHY7SI029211M

LAB668
foxtail_millet|11v3|
309
523

PHY7SI030817M

LAB670
foxtail_millet|11v3|
310
525

PHY7SI038207M

LAB671
foxtail_millet|11v3|
311
526

PHY7SI040241M

LAB672
gossypium_raimondii|12v1|
312
722

DR458820

LAB673
maize|10v1|AA979901
313
723

LAB675
maize|10v1|AF143681
314
529

LAB676
maize|10v1|AI372115
315
530

LAB677
maize|10v1|AI372353
316
724

LAB678
maize|10v1|AI619182
317
532

LAB679
maize|10v1|AI622384
318
533

LAB680
maize|10v1|AI622752
319
725

LAB681
maize|10v1|AI637066
320
726

LAB682
maize|10v1|AI677194
321
727

LAB683
maize|10v1|AI691657
322
537

LAB684
maize|10v1|AI734652
323
538

LAB685
maize|10v1|AI737066
324
539

LAB686
maize|10v1|AI737444
325
728

LAB687
maize|10v1|AI740094
326
541

LAB688
maize|10v1|AI795604
327
729

LAB689
maize|10v1|AI795742
328
543

LAB690
maize|10v1|AI795751
329
544

LAB691
maize|10v1|AI857184
330
730

LAB692
maize|10v1|AI861347
331
546

LAB693
maize|10v1|AI901930
332
547

LAB694
maize|10v1|AI902032
333
548

LAB695
maize|10v1|AI932122
334
549

LAB696
maize|10v1|AI947867
335
550

LAB697
maize|10v1|AI947885
336
551

LAB698
maize|10v1|AJ006547
337
731

LAB700
maize|10v1|AW066791
338
732

LAB701
maize|10v1|AW231459
339
554

LAB702
maize|10v1|AW330591
340
555

LAB703
maize|10v1|AW355959
341
733

LAB704
maize|10v1|AW461004
342
557

LAB705
maize|10v1|AW498362
343
734

LAB706
maize|10v1|AW499427
344
559

LAB707
maize|10v1|AW787305
345
735

LAB708
maize|10v1|AW787498
346
561

LAB709
maize|10v1|BE454007
347
562

LAB710
maize|10v1|BM378479
348
736

LAB711
maize|10v1|CD988313
349
564

LAB712
maize|10v1|CV985191
350
565

LAB713
maize|10v1|DN223227
351
737

LAB714
maize|10v1|T18853
352
567

LAB716
poplar|10v1|BU817192
353
568

LAB717
poplar|10v1|BU869216
354
569

LAB718
poplar|10v1|CA822891
355
570

LAB719
rice|11v1|AU062544
356
571

LAB721
rice|11v1|BE228839
357
738

LAB722
rice|11v1|BI305463
358
739

LAB723
rice|11v1|BI306005
359
575

LAB724
rice|11v1|BI796493
360
576

LAB725
rice|11v1|BI805675
361
740

LAB726
rice|11v1|BI805831
362
578

LAB727
rice|11v1|BI806975
363
741

LAB728
rice|11v1|BI807671
364
580

LAB729
rice|11v1|BM420747
365
742

LAB730
rice|11v1|C72652
366
582

LAB731
rice|11v1|CA766103
367
583

LAB732
rice|11v1|CB210565
368
584

LAB733
rice|11v1|CB210910
369
585

LAB734
rice|11v1|CI305545
370
586

LAB735
rice|11v1|CK040817
371
587

LAB736
rice|gb170|OS01G70010
372
743

LAB738
rice|gb170|OS05G30750
373
589

LAB739
rice|gb170|OS05G46500
374
590

LAB740

sorghum|11v1|SB03G000850
375
591

LAB741

sorghum|11v1|SB03G007380
376
592

LAB742

sorghum|11v1|SB09G004130
377
744

LAB746

sorghum|12v1|CF759842
378
595

LAB748

sorghum|12v1|SB01G002840
379
596

LAB749

sorghum|12v1|SB01G008190
380
745

LAB750

sorghum|12v1|SB01G025570
381
746

LAB751

sorghum|12v1|SB01G031145
382
747

LAB752

sorghum|12v1|SB01G035760
383
600

LAB753

sorghum|12v1|SB01G040510
384
748

LAB754

sorghum|12v1|SB01G049100
385
749

LAB755

sorghum|12v1|SB02G003700
386
603

LAB757

sorghum|12v1|SB02G028150
387
750

LAB758

sorghum|12v1|SB02G034770
388
606

LAB759

sorghum|12v1|SB02G039700
389
607

LAB760

sorghum|12v1|SB02G040060
390
608

LAB761

sorghum|12v1|SB03G005990
391
609

LAB762

sorghum|12v1|SB03G027180
392
610

LAB763

sorghum|12v1|SB03G030230
393
611

LAB764

sorghum|12v1|SB03G034230
394
751

LAB765

sorghum|12v1|SB03G034960
395
613

LAB767

sorghum|12v1|SB03G035060
396
614

LAB768

sorghum|12v1|SB03G038630
397
615

LAB769

sorghum|12v1|SB03G043250
398
616

LAB770

sorghum|12v1|SB04G004050
399
617

LAB771

sorghum|12v1|SB04G025650
400
618

LAB772

sorghum|12v1|SB04G027690
401
619

LAB773

sorghum|12v1|SB04G028210
402
620

LAB774

sorghum|12v1|SB05G005150
403
621

LAB775

sorghum|12v1|SB06G002090
404
622

LAB776

sorghum|12v1|SB06G017150
405
623

LAB777

sorghum|12v1|SB06G018340
406
624

LAB778

sorghum|12v1|SB06G018520
407
625

LAB779

sorghum|12v1|SB06G018670
408
626

LAB780

sorghum|12v1|SB06G019310
409
627

LAB781

sorghum|12v1|SB06G019625
410
628

LAB782

sorghum|12v1|SB06G021340
411
629

LAB783

sorghum|12v1|SB06G022690
412
630

LAB784

sorghum|12v1|SB06G026000
413
631

LAB785

sorghum|12v1|SB06G026210
414
632

LAB786

sorghum|12v1|SB06G029200
415
633

LAB787

sorghum|12v1|SB06G032650
416
634

LAB788

sorghum|12v1|SB07G024190
417
635

LAB789

sorghum|12v1|SB08G004890
418
752

LAB790

sorghum|12v1|SB08G006240
419
753

LAB791

sorghum|12v1|SB08G016450
420
638

LAB792

sorghum|12v1|SB09G002040
421
639

LAB793

sorghum|12v1|SB09G002180
422
640

LAB794

sorghum|12v1|SB09G004650
423
641

LAB795

sorghum|12v1|SB09G022090
424
642

LAB796

sorghum|12v1|SB09G022210
425
643

LAB797

sorghum|12v1|SB10G000500
426
644

LAB798

sorghum|12v1|SB10G029140
427
645

LAB799
sorghum|12v1|SB10G029750
428
646

LAB800
soybean|11v1|GLYMA04G05720
429
647

LAB801
soybean|11v1|GLYMA04G12170
430
648

LAB802
soybean|11v1|GLYMA04G00450
431
649

LAB803
soybean|11v1|GLYMA08G19920
432
650

LAB804
soybean|11v1|GLYMA10G01750
433
651

LAB805
soybean|11v1|GLYMA10G06620
434
652

LAB807
soybean|11v1|GLYMA10G38880
435
653

LAB809
soybean|11v1|GLYMA20G26610
436
754

LAB810
soybean|11v1|GLYMA20G34960
437
655

LAB811
soybean|11v1|GLYMA20G36400
438
656

LAB813
sunflower|10v1|CD852214
439
755

LAB814
sunflower|10v1|DY905846
440
756

LAB815
sunflower|10v1|DY920340
441
659

LAB816
sunflower|10v1|DY933583
442
757

LAB817
sunflower|12v1|CD849392
443
661

LAB820
sunflower|12v1|DY913292
444
758

LAB821
sunflower|12v1|DY915017
445
759

LAB823
sunflower|12v1|DY937266
446
760

LAB824
tomato|11v1|AI773686
447
665

LAB825
tomato|11v1|AI778113
448
761

LAB827
tomato|11v1|AW035009
449
667

LAB829
tomato|11v1|AW217661
450
668

LAB830
tomato|11v1|AW615872
451
669

LAB831
tomato|11v1|BG125080
452
670

LAB832
tomato|11v1|BG125441
453
762

LAB833
tomato|11v1|BG126165
454
672

LAB834
tomato|11v1|BG128255
455
673

LAB835
tomato|11v1|BG128279
456
674

LAB836
tomato|11v1|BG132326
457
675

LAB837
tomato|11v1|BG626094
458
763

LAB839
tomato|11v1|BG629779
459
677

LAB840
tomato|11v1|BG629817
460
678

LAB841
tomato|11v1|BG734916
461
764

LAB842
tomato|11v1|BQ512667
462
680

LAB843
tomato|11v1|CO751611
463
681

LAB844
wheat|10v2|AF079526
464
765

LAB845
wheat|10v2|BE405715
465
766

LAB847
wheat|10v2|BE637663
466
767

LAB848
wheat|10v2|BI479093
467
768

LAB849
wheat|10v2|BQ166387
468
769

LAB850
wheat|10v2|CK211432
469
770

LAB669_H7
rice|11v1|BE229317
470
688

LAB744_H1
maize|10v1|AI665228
471
689

LAB756_H3
rice|11v1|BU572354
472
690

LAB745

sorghum|12v1|CD462918
473
—

LAB747

sorghum|12v1|EVOER1621
474
—

Table 151. Provided are the identified genes which expression thereof in plants increases abiotic stress tolerance, water use efficiency, yield, growth rate, vigor, biomass, fiber yield, fiber quality, growth rate, oil content, nitrogen use efficiency and fertilizer use efficiency of a plant. “Polyn.”—polynucleotide; “Polyp.”—polypeptide.

Example 19
Identification of Homologues which Affect Abst, Wue, Yield, Growth Rate, Vigor, Biomass, Oil Content, Nue and/or Fue of a Plant

The concepts of orthology and paralogy have recently been applied to functional characterizations and classifications on the scale of whole-genome comparisons. Orthologs and paralogs constitute two major types of homologs: The first evolved from a common ancestor by specialization, and the latter are related by duplication events. It is assumed that paralogs arising from ancient duplication events are likely to have diverged in function while true orthologs are more likely to retain identical function over evolutionary time.

Identification of putative orthologs of the genes identified in Table 151 above can be performed using various tools such as the BLAST (/Basic Local Alignment Search Tool/). Sequences sufficiently similar were tentatively grouped. These putative orthologs were further organized under a Phylogram—a branching diagram (tree) assumed to be a representation of the evolutionary relationships among the biological taxa. Putative ortholog groups were analyzed as to their agreement with the phylogram and in cases of disagreements these ortholog groups were broken accordingly.

Expression data was analyzed and the EST libraries were classified using a fixed vocabulary of custom terms such as developmental stages (e.g., genes showing similar expression profile through development with up regulation at specific stage, such as at the seed filling stage) and/or plant organ (e.g., genes showing similar expression profile across their organs with up regulation at specific organs such as seed). The annotations from all the ESTs clustered to a gene were analyzed statistically by comparing their frequency in the cluster versus their abundance in the database, allowing to construct a numeric and graphic expression profile of that gene, which is termed “digital expression”. The rationale of using these two complementary methods with methods of phenotypic association studies of QTLs, SNPs and phenotype expression correlation is based on the assumption that true orthologs are likely to retain identical function over evolutionary time. These methods provide different sets of indications on function similarities between two homologous genes, similarities in the sequence level—identical amino acids in the protein domains and similarity in expression profiles.

Methods for searching and identifying homologues of yield and improved agronomic traits such as ABS tolerance and FUE related polypeptides or polynucleotides are well within the realm of the skilled artisan. The search and identification of homologous genes involves the screening of sequence information available, for example, in public databases, which include but are not limited to the DNA Database of Japan (DDBJ), Genbank, and the European Molecular Biology Laboratory Nucleic Acid Sequence Database (EMBL) or versions thereof or the MIPS database. A number of different search algorithms have been developed, including but not limited to the suite of programs referred to as BLAST programs. There are five implementations of BLAST, three designed for nucleotide sequence queries (BLASTN, BLASTX, and TBLASTX) and two designed for protein sequence queries (BLASTP and TBLASTN) (Coulson, Trends in Biotechnology: 76-80, 1994; Birren et al., Genome Analysis, I: 543, 1997). Such methods involve alignment and comparison of sequences. The BLAST algorithm calculates percent sequence identity and performs a statistical analysis of the similarity between the two sequences. The software for performing BLAST analysis is publicly available through the National Centre for Biotechnology Information. Other such software or algorithms are GAP, BESTFIT, FASTA and TFASTA. GAP uses the algorithm of Needleman and Wunsch (J. Mol. Biol. 48: 443-453, 1970) to find the alignment of two complete sequences that maximizes the number of matches and minimizes the number of gaps.

The homologous genes may belong to the same gene family. The analysis of a gene family may be carried out using sequence similarity analysis. To perform this analysis one may use standard programs for multiple alignments e.g. Clustal W. A neighbour joining tree of the proteins homologous to the genes in this invention may be used to provide an overview of structural and ancestral relationships. Sequence identity may be calculated using an alignment program as described above. It is expected that other plants will carry a similar functional gene (orthologue) or a family of similar genes and those genes will provide the same preferred phenotype as the genes presented here. Advantageously, these family members may be useful in the methods of the invention. Example of other plants are included here but not limited to, barley (Hordeum vulgare), Arabidopsis (Arabidopsis thaliana), maize (Zea mays), cotton (Gossypium), Oilseed rape (Brassica napus), Rice (Oryza sativa), Sugar cane (Saccharum officinarum), Sorghum (Sorghum bicolor), Soybean (Glycine max), Sunflower (Helianthus annuus), Tomato (Lycopersicon esculentum), Wheat (Triticum aestivum).

The above-mentioned analyses for sequence homology is preferably carried out on a full-length sequence, but may also be based on a comparison of certain regions such as conserved domains The identification of such domains would also be well within the realm of the person skilled in the art and would involve, for example, a computer readable format of the nucleic acids of the present invention, the use of alignment software programs and the use of publicly available information on protein domains, conserved motifs and boxes. This information is available in the PRODOM (Hypertext Transfer Protocol://World Wide Web (dot) biochem (dot) ucl (dot) ac (dot) uk/bsm/dbbrowser/protocol/prodomqry (dot) html), PIR (Hypertext Transfer Protocol://pir (dot) Georgetown (dot) edu/) or Pfam (Hypertext Transfer Protocol://World Wide Web (dot) sanger (dot) ac (dot) uk/Software/Pfam/) database. Sequence analysis programs designed for motif searching may be used for identification of fragments, regions and conserved domains as mentioned above. Preferred computer programs include, but are not limited to, MEME, SIGNALSCAN, and GENESCAN.

A person skilled in the art may use the homologous sequences provided herein to find similar sequences in other species and other organisms. Homologues of a protein encompass, peptides, oligopeptides, polypeptides, proteins and enzymes having amino acid substitutions, deletions and/or insertions relative to the unmodified protein in question and having similar biological and functional activity as the unmodified protein from which they are derived. To produce such homologues, amino acids of the protein may be replaced by other amino acids having similar properties (conservative changes, such as similar hydrophobicity, hydrophilicity, antigenicity, propensity to form or break a-helical structures or 3-sheet structures). Conservative substitution tables are well known in the art (see for example Creighton (1984) Proteins. W.H. Freeman and Company). Homologues of a nucleic acid encompass nucleic acids having nucleotide substitutions, deletions and/or insertions relative to the unmodified nucleic acid in question and having similar biological and functional activity as the unmodified nucleic acid from which they are derived.

Polynucleotides and polypeptides with significant homology to the identified genes described in Table 151 (Example 18 above) were identified from the databases using BLAST software with the Blastp and tBlastn algorithms as filters for the first stage, and the needle (EMBOSS package) or Frame+ algorithm alignment for the second stage. Local identity (Blast alignments) was defined with a very permissive cutoff—60% Identity on a span of 60% of the sequences lengths because it use as only a filter for the global alignment stage. The default filtering of the Blast package was not utilized (by setting the parameter “-F F”).

In the second stage, homologs were defined based on a global identity of at least 80% to the core gene polypeptide sequence. Two distinct forms for finding the optimal global alignment for protein or nucleotide sequences were used in this application:

1. Between two proteins (following the blastp filter): EMBOSS-6.0.1 Needleman-Wunsch algorithm with the following modified parameters: gapopen=8 gapextend=2. The rest of the parameters were unchanged from the default options described hereinabove. 2. Between a protein sequence and a nucleotide sequence (following the tblastn filter):

GenCore 6.0 OneModel application utilizing the Frame+ algorithm with the following parameters: model=frame+_p2n.model mode=qglobal -q=protein. sequence -db=nucleotide.sequence. The rest of the parameters are unchanged from the default options described hereinabove.

The query polypeptide sequences were SEQ ID NOs: 475-770 [which are encoded by the polynucleotides SEQ ID NOs:1-474 shown in Table 151) above] and the identified orthologous and homologous sequences having at least 80% global sequence identity are provided in Table 152, below. These homologous genes are expected to increase plant ABST, yield, seed yield, oil yield, oil content, growth rate, fiber yield, fiber quality, fiber length, photosynthetic capacity, biomass, vigor, WUE and/or NUE of a plant.

TABLE 152

Homologues of the identified genes/polypeptides for increasing abiotic stress tolerance,

water use efficiency, yield, growth rate, vigor, oil content, biomass, growth rate,

nitrogen use efficiency and fertilizer use efficiency of a plant

Polyn.
Polyp.
Hom. to
%

Homologue

SEQ ID
SEQ ID
SEQ ID
Glob.

Name
Cluster name
NO:
NO:
NO:
Ident.
Algor.

LAB616_H1
arabidopsis_lyrata|09v1|JGIA
771
6179
475
88.45
glotblastn

L008105_T1

LAB617_H1
arabidopsis_lyrata|09v1|JGIA
772
6180
476
93.4
globlastp

L019605_P1

LAB617_H2
b_rapa|11v1|CD816899_P1
773
6181
476
91.5
globlastp

LAB617_H3
canola|11v1|CB331895_P1
774
6181
476
91.5
globlastp

LAB617_H4
canola|11v1|SRR001111.1354
775
6181
476
91.5
globlastp

0_P1

LAB804_H1
thellungiella_parvulum|11v1|
776
6182
476
91.5
globlastp

BY803956

LAB804_H1
thellungiella_parvulum|11v1|
776
6182
651
80.3
globlastp

BY803956

LAB617_H6
b_juncea|10v2|E6ANDIZ01A
777
6183
476
91.1
globlastp

5S0C

LAB617_H7
canola|11v1|CN828959_P1
778
6184
476
91.1
globlastp

LAB617_H8
thellungiella_halophilum|11v
779
6185
476
91.1
globlastp

1|BY803956

LAB617_H14
b_juncea|12v1|E6ANDIZ01A
780
6186
476
90.6
globlastp

URJS_P1

LAB617_H9
b_oleracea|gb161|EH422504_—
781
6187
476
90.6
globlastp

P1

LAB617_H15
b_juncea|12v1|E6ANDIZ01B
782
6188
476
90.1
globlastp

WTOD_P1

LAB617_H10
radish|gb164|EV527182
783
6189
476
90.1
globlastp

LAB617_H11
radish|gb164|EV567824
784
6190
476
90.1
globlastp

LAB617_H12
radish|gb164|FD538955
785
6191
476
89.2
glotblastn

LAB617_H13
radish|gb164|EV537654
786
6192
476
88.3
globlastp

LAB804_H2
clementine|11v1|CF417156_P
787
6193
476
83.6
globlastp

1

LAB804_H2
clementine|11v1|CF417156_P
787
6193
651
85.9
globlastp

1

LAB804_H3
orange|11v1|CF417156_P1
788
6193
476
83.6
globlastp

LAB804_H3
orange|11v1|CF417156_P1
788
6193
651
85.9
globlastp

LAB804_H4
cleome_spinosa|10v1|GR9324
789
6194
476
83.3
globlastp

68_P1

LAB804_H4
cleome_spinosa|10v1|GR9324
789
6194
651
80.9
globlastp

68_P1

LAB804_H5
cleome_spinosa|10v1|SRR015
790
6195
476
83.1
globlastp

531S0006992_P1

LAB804_H5
cleome_spinosa|10v1|SRR015
790
6195
651
80.8
globlastp

531S0006992_P1

LAB804_H6
cacao|10v1|CF973443_P1
791
6196
476
82.2
globlastp

LAB804_H6
cacao|10v1|CF973443_P1
791
6196
651
81.7
globlastp

LAB804_H7
peanut|10v1|EG029690_P1
792
6197
476
81.8
globlastp

LAB804_H7
peanut|10v1|EG029690_P1
792
6197
651
89.2
globlastp

LAB804_H8
euonymus|11v1|SRR070038X
793
6198
476
81.7
globlastp

120018_P1

LAB804_H8
euonymus|11v1|SRR070038X
793
6198
651
85.4
globlastp

120018_P1

LAB804_H9
tripterygium|11v1|SRR09867
794
6199
476
81.7
globlastp

7X109347

LAB804_H9
tripterygium|11v1|SRR09867
794
6199
651
85
globlastp

7X109347

LAB804_H10
pteridium|11v1|SRR043594X
795
6200
476
81.69
glotblastn

142729

LAB804_H10
pteridium|11v1|SRR043594X
795
6200
651
81.22
glotblastn

142729

LAB804_H11
oak|10v1|CU656480_P1
796
6201
476
81.3
globlastp

LAB804_H11
oak|10v1|CU656480_P1
796
6201
651
85
globlastp

LAB804_H12
cotton|11v1|AW186702_P1
797
6202
476
81.2
globlastp

LAB804_H12
cotton|11v1|AW186702_P1
797
6202
651
81.7
globlastp

LAB804_H13
gossypium_raimondii|12v1|A
798
6202
476
81.2
globlastp

W186702_P1

LAB804_H13
gossypium_raimondii|12v1|A
798
6202
651
81.7
globlastp

W186702_P1

LAB804_H14
tripterygium|11v1|SRR09867
799
6203
476
81.2
globlastp

7X104467XX2

LAB804_H14
tripterygium|11v1|SRR09867
799
6203
651
85
globlastp

7X104467XX2

LAB804_H15
cirsium|11v1|SRR346952.105
800
6204
476
80.8
globlastp

939_P1

LAB804_H15
cirsium|11v1|SRR346952.105
800
6204
651
88
globlastp

939_P1

LAB804_H16
cynara|gb167|GE592518_P1
801
6205
476
80.8
globlastp

LAB804_H16
cynara|gb167|GE592518_P1
801
6205
651
87.5
globlastp

LAB804_H17
eucalyptus|11v2|CU395771_P
802
6206
476
80.8
globlastp

1

LAB804_H17
eucalyptus|11v2|CU395771_P
802
6206
651
85.9
globlastp

1

LAB804_H18
grape|11v1|GSVIVT0102810
803
6207
476
80.8
globlastp

0001_P1

LAB804_H18
grape|11v1|GSVIVT0102810
803
6207
651
85.4
globlastp

0001_P1

LAB804_H19
humulus|11v1|ES654374_P1
804
6208
476
80.8
globlastp

LAB804_H19
humulus|11v1|ES654374_P1
804
6208
651
89.2
globlastp

LAB804_H20
platanus|11v1|SRR096786X1
805
6209
476
80.8
globlastp

09993_P1

LAB804_H20
platanus|11v1|SRR096786X1
805
6209
651
83.1
globlastp

09993_P1

LAB804_H21
cannabis|12v1|EW700651_P1
806
6210
476
80.3
globlastp

LAB804_H21
cannabis|12v1|EW700651_P1
806
6210
651
88.3
globlastp

LAB804_H22
hornbeam|12v1|SRR364455.1
807
6211
476
80.3
globlastp

12836_P1

LAB804_H22
hornbeam|12v1|SRR364455.1
807
6211
651
85.9
globlastp

12836_P1

LAB804_H23
papaya|gb165|CO373908_P1
808
6212
476
80.3
globlastp

LAB804_H23
papaya|gb165|CO373908_P1
808
6212
651
82.6
globlastp

LAB804_H24
pigeonpea|11v1|GR472234_P
809
6213
476
80.3
globlastp

1

LAB804_H24
pigeonpea|11v1|GR472234_P
809
6213
651
96.2
globlastp

1

LAB804_H25
safflower|gb162|EL373692
810
6214
476
80.3
globlastp

LAB804_H25
safflower|gb162|EL373692
810
6214
651
86.1
globlastp

LAB618_H1
canola|11v1|EE411660_T1
811
6215
477
87.54
glotblastn

LAB618_H2
radish|gb164|EX757800
812
6216
477
87.3
globlastp

LAB618_H3
thellungiella_halophilum|11v
813
6217
477
86.8
globlastp

1|EHJGI11016416

LAB618_H4
thellungiella_parvulum|11v1|
814
6218
477
86.4
globlastp

EPCRP030814

LAB618_H5
b_rapa|11v1|DY027925_P1
815
6219
477
86.3
globlastp

LAB618_H6
arabidopsis_lyrata|09v1|JGIA
816
6220
477
85.3
globlastp

L026042_P1

LAB618_H7
canola|11v1|EE513334_P1
817
6221
477
82.8
globlastp

LAB618_H8
b_juncea|12v1|E6ANDIZ01E
818
6222
477
81.96
glotblastn

PONR_T1

LAB619_H4
b_juncea|10v2|E6ANDIZ01A
819
6223
478
86.1
globlastp

OTZC

LAB619_H5
radish|gb164|EX746885
820
6224
478
86.1
globlastp

LAB619_H9
b_juncea|12v1|E6ANDIZ01A
821
6225
478
85.4
globlastp

OTZC_P1

LAB619_H6
canola|11v1|ES977584_P1
822
6226
478
85.4
globlastp

LAB619_H7
b_oleracea|gb161|EH429338_—
823
6227
478
85.13
glotblastn

T1

LAB619_H8
b_rapa|11v1|DY013352_P1
824
6228
478
85.1
globlastp

LAB620_H2
wheat|12v3|BE445227_P1
825
6229
479
96
globlastp

LAB620_H1
rye|12v1|DRR001012.468942
826
6230
479
95.8
globlastp

LAB620_H2
wheat|10v2|BE426572
827
6231
479
95.5
globlastp

LAB620_H11
wheat|12v3|BE426572_P1
828
6232
479
95.5
globlastp

LAB620_H3
rye|12v1|DRR001012.133035
829
6233
479
95.01
glotblastn

LAB620_H4
rye|12v1|DRR001013.17335
830
6234
479
91.4
globlastp

LAB620_H5
rye|12v1|DRR001012.103279
831
6235
479
90.6
globlastp

LAB620_H6
rye|12v1|DRR001012.137158
832
6236
479
89.2
globlastp

LAB620_H7
oat|11v1|GO596517_P1
833
6237
479
89.1
globlastp

LAB620_H8
rye|12v1|BE637364
834
6238
479
88.7
globlastp

LAB620_H9
brachypodium|12v1|BRADI5
835
6239
479
88
globlastp

G25610_P1

LAB620_H10
leymus|gb166|EG376080_P1
836
6240
479
87.4
globlastp

LAB620_H11
wheat|10v2|BE442673
837
6241
479
86.2
globlastp

LAB620_H12
rice|11v1|BE228459
838
6242
479
84.2
globlastp

LAB620_H13
rice|11v1|CF568779
839
6243
479
84.1
globlastp

LAB620_H14
switchgrass|gb167|FE614464
840
6244
479
82
globlastp

LAB620_H15
foxtail_millet|11v3|PHY7SI0
841
6245
479
81.9
globlastp

22133M_P1

LAB620_H14
switchgrass|12v1|FE614464_—
842
6246
479
81.8
globlastp

P1

LAB620_H16
sorghum|12v1|SB06G032270
843
6247
479
81.2
globlastp

LAB620_H17
maize|10v1|AW165436_P1
844
6248
479
80.7
globlastp

LAB621_H1
wheat|10v2|BE638019
845
6249
480
96.9
globlastp

LAB621_H1
wheat|12v3|CV771505_P1
846
6250
480
96.9
globlastp

LAB621
barley|12v1|BG299493_P1
847
6251
480
88.4
globlastp

LAB621_H4
brachypodium|12v1|BRADI1
848
6252
480
82.6
globlastp

G51940T2_P1

LAB621_H2
rye|12v1|DRR001012.247676
849
6253
480
82.5
globlastp

LAB622_H8
rice|11v1|C97877
850
6254
481
84.7
globlastp

LAB622_H10
foxtail_millet|11v3|PHY7SI0
851
6255
481
81.5
globlastp

10927M_P1

LAB623_H1
rye|12v1|DRR001012.122581
852
6256
482
94.6
globlastp

LAB623_H2
rice|11v1|AU056684
853
6257
482
85.3
globlastp

LAB623_H8
barley|12v1|BG416095_P1
854
6258
482
84.6
globlastp

LAB623_H3
foxtail_millet|11v3|PHY7SI0
855
6259
482
84.6
globlastp

29505M_P1

LAB623_H9
switchgrass|12v1|FL691409_—
856
6260
482
84.2
globlastp

P1

LAB623_H4
sorghum|12v1|SB02G025760
857
6261
482
82.7
globlastp

LAB623_H5
sugarcane|10v1|CA075318
858
6262
482
82.3
globlastp

LAB623_H10
wheat|12v3|BE419298_P1
859
6263
482
81.4
globlastp

LAB623_H6
maize|10v1|AI586808_P1
860
6264
482
81.4
globlastp

LAB623_H7
maize|10v1|AI622596_P1
861
6265
482
80.8
globlastp

LAB624_H4
wheat|12v3|BE500006_P1
862
6266
483
96.9
globlastp

LAB624_H5
wheat|12v3|SRR073321X152
863
6267
483
96.9
globlastp

149D1_P1

LAB624_H3
brachypodium|12v1|BRADI4
864
6268
483
86.4
globlastp

G16810_P1

LAB624_H6
rice|11v1|CI224808_P1
865
6269
483
82.4
globlastp

LAB624_H7
sorghum|12v1|SB05G020900_P
866
6270
483
82
globlastp

1

LAB624_H8
foxtail_millet|11v3|PHY7SI0
867
6271
483
81.8
globlastp

26426M_P1

LAB625_H1
leymus|gb166|EG391780_P1
868
6272
484
99.1
globlastp

LAB625_H2
rye|12v1|DRR001012.127623
869
6273
484
99.1
globlastp

LAB625_H3
wheat|10v2|BQ788819
870
6274
484
98.8
globlastp

LAB625_H3
wheat|12v3|BQ789482_P1
871
6274
484
98.8
globlastp

LAB625_H4
brachypodium|12v1|BRADI1
872
6275
484
94.8
globlastp

G15940_P1

LAB625_H5
switchgrass|gb167|FE636459
873
6276
484
92.8
globlastp

LAB625_H5
switchgrass|12v1|FE636459_P1
874
6277
484
92.5
globlastp

LAB625_H6
maize|10v1|AI491622_P1
875
6278
484
92.2
globlastp

LAB625_H7
rice|11v1|BE040825
876
6279
484
92.2
globlastp

LAB625_H8
sorghum|12v1|SB10G002920
877
6280
484
92.2
globlastp

LAB625_H9
cenchrus|gb166|EB661083_P
878
6281
484
91.6
globlastp

1

LAB625_H10
sugarcane|10v1|CA089197
879
6282
484
90.8
globlastp

LAB625_H11
millet|10v1|EVO454PM0551
880
6283
484
90.5
globlastp

99_P1

LAB625_H12
foxtail_millet|11v3|PHY7SI0
881
6284
484
90.17
glotblastn

36501M_T1

LAB625_H13
phalaenopsis|11v1|SRR12577
882
6285
484
84.4
globlastp

1.101382_P1

LAB625_H77
prunus_mume|13v1|DW3446
883
6286
484
83.5
globlastp

62_P1

LAB625_H14
aristolochia|10v1|FD760040_—
884
6287
484
83.5
globlastp

P1

LAB625_H15
papaya|gb165|EX238938_P1
885
6288
484
83.5
globlastp

LAB625_H16
prunus|10v1|CN494803
886
6286
484
83.5
globlastp

LAB625_H78
sesame|12v1|BU670022_P1
887
6289
484
82.9
globlastp

LAB625_H17
apple|11v1|CN494803_P1
888
6290
484
82.9
globlastp

LAB625_H18
cassava|09v1|DV451804_P1
889
6291
484
82.9
globlastp

LAB625_H19
clementine|11v1|CV715595_—
890
6292
484
82.9
globlastp

P1

LAB625_H20
eucalyptus|11v2|SRR001658
891
6293
484
82.9
glotblastn

X5147_T1

LAB625_H21
oil_palm|11v1|SRR190698.27
892
6294
484
82.9
globlastp

2408_P1

LAB625_H22
orange|11v1|CV715595_P1
893
6292
484
82.9
globlastp

LAB625_H23
castorbean|12v1|EG672819_P
894
6295
484
82.4
globlastp

1

LAB625_H24
radish|gb164|EV525521
895
6296
484
82.4
globlastp

LAB625_H25
amsonia|11v1|SRR098688X1
896
6297
484
82.1
globlastp

26499_P1

LAB625_H26
cotton|11v1|DT553596_P1
897
6298
484
82.1
globlastp

LAB625_H27
watermelon|11v1|DV633645
898
6299
484
82.1
globlastp

LAB625_H28
maritime_pine|10v1|BX25421
899
6300
484
82
globlastp

2_P1

LAB625_H29
peanut|10v1|EE124863_P1
900
6301
484
82
globlastp

LAB625_H30
pine|10v2|AA739796_P1
901
6300
484
82
globlastp

LAB625_H31
spruce|11v1|ES252241
902
6302
484
82
globlastp

LAB625_H32
triphysaria|10v1|EY172940
903
6303
484
82
globlastp

LAB625_H79
aquilegia|10v2|DT733421_P1
904
6304
484
81.8
globlastp

LAB625_H33
aquilegia|10v1|DT733421
905
6304
484
81.8
globlastp

LAB625_H34
nicotiana_benthamiana|12v1|
906
6305
484
81.8
globlastp

CN742800_P1

LAB625_H34
nicotiana_benthamiana|gb162|
907
6306
484
81.8
globlastp

CN742800

LAB625_H35
poppy|11v1|SRR030259.1225
908
6307
484
81.8
globlastp

51_P1

LAB625_H36
phyla|11v2|SRR099035X163
909
6308
484
81.74
glotblastn

195_T1

LAB625_H37
tabernaemontana|11v1|SRR09
910
6309
484
81.7
globlastp

8689X125206

LAB625_H80
banana|12v1|FF558823_P1
911
6310
484
81.5
globlastp

LAB625_H38
b_rapa|11v1|CN732059_P1
912
6311
484
81.5
globlastp

LAB625_H39
chestnut|gb170|SRR006295S0
913
6312
484
81.5
globlastp

019697_P1

LAB625_H40
oak|10v1|FP042989_P1
914
6312
484
81.5
globlastp

LAB625_H41
tomato|11v1|BG132037
915
6313
484
81.5
globlastp

LAB625_H42
beech|11v1|FR617570_T1
916
6314
484
81.45
glotblastn

LAB625_H43
sarracenia|11v1|SRR192669.1
917
6315
484
81.45
glotblastn

58321

LAB625_H81
bean|12v2|CA896913_P1
918
6316
484
81.3
globlastp

LAB625_H82
lettuce|12v1|DW111551_P1
919
6317
484
81.3
globlastp

LAB625_H44
bean|12v1|CA896913
920
6316
484
81.3
globlastp

LAB625_H45
cowpea|12v1|FF401664_P1
921
6318
484
81.3
globlastp

LAB625_H45
cowpea|gb166|FF401664
922
6318
484
81.3
globlastp

LAB625_H46
lettuce|10v1|DW111551
923
6317
484
81.3
globlastp

LAB625_H83
poplar|13v1|CK112093_P1
924
6319
484
81.2
globlastp

LAB625_H47
monkeyflower|10v1|GR02129
925
6320
484
81.2
globlastp

9

LAB625_H47
monkeyflower|12v1|SRR0372
926
6320
484
81.2
globlastp

27.100352_P1

LAB625_H48
poplar|10v1|CK112093
927
6319
484
81.2
globlastp

LAB625_H49
poplar|10v1|XM002313872
928
6321
484
81.2
globlastp

LAB625_H49
poplar|13v1|XM_002313872_—
929
6321
484
81.2
globlastp

P1

LAB625_H50
potato|10v1|BG591935_P1
930
6322
484
81.2
globlastp

LAB625_H51
solanum_phureja|09v1|SPHB
931
6322
484
81.2
globlastp

G132037

LAB625_H52
strawberry|11v1|EX670890
932
6323
484
81.2
globlastp

LAB625_H84
blueberry|12v1|SRR353282X
933
6324
484
81.16
glotblastn

62159D1_T1

LAB625_H53
beet|12v1|BI543970_P1
934
6325
484
81.1
globlastp

LAB625_H54
soybean|11v1|GLYMA10G40
935
6326
484
81
globlastp

440

LAB625_H54
soybean|12v1|GLYMA10G40
936
6326
484
81
globlastp

440_P1

LAB625_H55
cowpea|12v1|FC461332_P1
937
6327
484
80.9
globlastp

LAB625_H55
cowpea|gb166|FC461332
938
6327
484
80.9
globlastp

LAB625_H56
medicago|12v1|AW980662_P
939
6328
484
80.9
globlastp

1

LAB625_H57
thellungiella_parvulum|11v1|
940
6329
484
80.9
globlastp

EPCRP010044

LAB625_H58
euonymus|11v1|SRR070038X
941
6330
484
80.87
glotblastn

15434_T1

LAB625_H59
thellungiella_halophilum|11v
942
6331
484
80.87
glotblastn

1|EHJGI11005074

LAB625_H60
thellungiella_parvulum|11v1|
943
6332
484
80.87
glotblastn

EPCRP005066

LAB625_H61
cotton|11v1|DT548734_P1
944
6333
484
80.6
globlastp

LAB625_H62
cycas|gb166|EX922356_P1
945
6334
484
80.6
globlastp

LAB625_H63
pigeonpea|11v1|SRR054580X
946
6335
484
80.6
globlastp

423669_P1

LAB625_H64
soybean|11v1|GLYMA20G26
947
6336
484
80.35
glotblastn

890

LAB625_H64
soybean|12v1|GLYMA20G26
948
6336
484
80.35
glotblastn

890_T1

LAB625_H65
catharanthus|11v1|SRR09869
949
6337
484
80.3
globlastp

1X103228_P1

LAB625_H66
flax|11v1|JG122552_P1
950
6338
484
80.3
globlastp

LAB625_H67
gossypium_raimondii|12v1|D
951
6339
484
80.3
globlastp

T548734_P1

LAB625_H68
ipomoea_nil|10v1|BJ562192_—
952
6340
484
80.3
globlastp

P1

LAB625_H69
antirrhinum|gb166|AJ793853
953
6341
484
80.29
glotblastn

_T1

LAB625_H70
cirsium|11v1|SRR346952.106
954
6342
484
80.29
glotblastn

990_T1

LAB625_H71
cirsium|11v1|SRR346952.833
955
6343
484
80.29
glotblastn

569_T1

LAB625_H72
silene|11v1|SRR096785X102
956
6344
484
80.29
glotblastn

281

LAB625_H73
grape|11v1|GSVIVT0103784
957
6345
484
80.2
globlastp

8001_P1

LAB625_H74
chickpea|11v1|SRR133517.12
958
6346
484
80.1
globlastp

3706

LAB625_H74
chickpea|13v2|SRR133517.12
959
6346
484
80.1
globlastp

3706_P1

LAB625_H75
arabidopsis_lyrata|09v1|JGIA
960
6347
484
80
glotblastn

L010505_T1

LAB625_H76
lotus|09v1|AW719379_T1
961
6348
484
80
glotblastn

LAB626_H1
pseudoroegneria|gb167|FF349
962
6349
485
98.7
globlastp

328

LAB626_H2
rye|12v1|BE495583
963
6350
485
98.3
globlastp

LAB626_H3
wheat|10v2|BE419997
964
6351
485
98
globlastp

LAB626_H3
wheat|12v3|BE419997_P1
965
6351
485
98
globlastp

LAB626_H4
leymus|gb166|EG374735_P1
966
6352
485
97.7
globlastp

LAB626_H5
brachypodium|12v1|BRADI5
967
6353
485
92.1
globlastp

G12080_P1

LAB626_H6
rice|11v1|AU029951
968
6354
485
86.6
globlastp

LAB626_H7
maize|10v1|CB281936_P1
969
6355
485
83.8
globlastp

LAB626_H8
sorghum|12v1|SB06G018640
970
6356
485
83.8
globlastp

LAB626_H9
sugarcane|10v1|CA074242
971
6357
485
83.8
globlastp

LAB626_H10
maize|10v1|AI891360_P1
972
6358
485
83.5
globlastp

LAB626_H14
switchgrass|12v1|FL742760_—
973
6359
485
83.2
globlastp

P1

LAB626_H11
fescue|gb161|DT675496_P1
974
6360
485
83.2
globlastp

LAB626_H12
foxtail_millet|11v3|PHY7SI0
975
6361
485
83.2
globlastp

10696M_P1

LAB626_H13
millet|10v1|EVO454PM0029
976
6362
485
82.6
globlastp

55_P1

LAB626_H15
switchgrass|12v1|FL756782_—
977
6363
485
82.5
globlastp

P1

LAB628_H1
wheat|10v2|BE590956
978
6364
487
93.2
globlastp

LAB628_H1
wheat|12v3|BE590956_P1
979
6364
487
93.2
globlastp

LAB628_H2
pseudoroegneria|gb167|FF362
980
6365
487
91.1
globlastp

016

LAB628_H3
fescue|gb161|DT685079_P1
981
6366
487
84.8
globlastp

LAB628_H4
brachypodium|12v1|BRADI2
982
6367
487
82.7
globlastp

G43040_P1

LAB629_H2
wheat|12v3|BG274995_P1
983
6368
488
94.6
globlastp

LAB629_H1
rye|12v1|DRR001012.227452
984
6369
488
94.23
glotblastn

LAB630_H16
wheat|12v3|BE497431_P1
985
6370
489
96.8
globlastp

LAB630_H1
pseudoroegneria|gb167|FF364
986
6371
489
96.8
globlastp

958

LAB630_H2
wheat|10v2|BE497431XX2
987
6372
489
96.8
globlastp

LAB630_H2
wheat|12v3|BE517187_P1
988
6373
489
96.5
globlastp

LAB630_H3
rye|12v1|DRR001012.11011
989
6374
489
95.9
globlastp

LAB630_H4
rye|12v1|DRR001012.115392
990
6375
489
95.3
globlastp

LAB630_H5
rye|12v1|DRR001012.497471
991
6376
489
94.75
glotblastn

LAB630_H6
brachypodium|12v1|BRADI4
992
6377
489
91.6
globlastp

G02350_P1

LAB630_H7
foxtail_millet|11v3|GT091056
993
6378
489
84.5
globlastp

_P1

LAB630_H8
rice|11v1|AA751964
994
6379
489
83.7
globlastp

LAB630_H9
maize|10v1|AI395949_P1
995
6380
489
83.4
globlastp

LAB630_H10
maize|10v1|AI396380_P1
996
6381
489
83.4
globlastp

LAB630_H11
millet|10v1|EVO454PM0136
997
6382
489
83.3
globlastp

72_P1

LAB630_H12
sorghum|12v1|SB08G020830
998
6383
489
83.1
globlastp

LAB630_H13
switchgrass|gb167|FE625960
999
6384
489
83.1
globlastp

LAB630_H14
oat|11v1|CN818230_P1
1000
6385
489
82.8
globlastp

LAB630_H17
switchgrass|12v1|FE608986_—
1001
6386
489
82.2
globlastp

P1

LAB630_H15
sugarcane|10v1|BQ537487
1002
6387
489
81.7
globlastp

LAB631_H1
rye|12v1|DRR001012.353645
1003
6388
490
93
globlastp

LAB631_H2
rye|12v1|DRR001012.773201
1004
6388
490
93
globlastp

LAB631_H3
wheat|10v2|BE425328
1005
6389
490
93
globlastp

LAB631_H3
wheat|12v3|BE425328_P1
1006
6389
490
93
globlastp

LAB631_H4
rye|12v1|DRR001012.14856
1007
6390
490
92.6
globlastp

LAB631_H5
rye|12v1|DRR001012.272200
1008
6391
490
90.7
globlastp

LAB632_H9
wheat|12v3|BM135360_P1
1009
6392
491
95.1
globlastp

LAB632_H1
wheat|10v2|BE591422
1010
6393
491
95.1
globlastp

LAB632_H1
wheat|12v3|BE591422_P1
1011
6393
491
95.1
globlastp

LAB632_H2
rye|12v1|DRR001012.102621
1012
6394
491
94.4
globlastp

LAB632_H3
oat|11v1|CN817514_P1
1013
6395
491
88.5
globlastp

LAB632_H4
fescue|gb161|CK803085_P1
1014
6396
491
87
globlastp

LAB632_H5
brachypodium|12v1|BRADI1
1015
6397
491
86.6
globlastp

G15400_P1

LAB632_H6
fescue|gb161|CK802979_P1
1016
6398
491
86.6
globlastp

LAB632_H7
rye|12v1|DRR001012.138316
1017
6399
491
85.5
globlastp

LAB632_H8
lolium|10v1|AU250959_T1
1018
6400
491
82.84
glotblastn

LAB633_H84
wheat|12v3|BE415525_P1
1019
492
492
100
globlastp

6

LAB633_H84
wheat|12v3|BE497510_P1
1020
492
492
100
globlastp

7

LAB633_H1
fescue|gb161|DT688127_P1
1021
492
492
100
globlastp

LAB633_H2
leymus|gb166|CD808938_P1
1022
492
492
100
globlastp

LAB633_H3
lolium|10v1|AU247803_P1
1023
492
492
100
globlastp

LAB633_H4
lolium|10v1|AU250131_P1
1024
492
492
100
globlastp

LAB633_H5
lolium|10v1|DT670744_P1
1025
492
492
100
globlastp

LAB633_H6
oat|11v1|ASTATPASEH_P1
1026
492
492
100
globlastp

LAB633_H7
oat|11v1|CN816203_P1
1027
492
492
100
globlastp

LAB633_H8
oat|11v1|CN818473_P1
1028
492
492
100
globlastp

LAB633_H9
oat|11v1|GR326071_P1
1029
492
492
100
globlastp

LAB633_H10
pseudoroegneria|gb167|FF342
1030
492
492
100
globlastp

202

LAB633_H11
pseudoroegneria|gb167|FF354
1031
492
492
100
globlastp

282

LAB633_H12
rye|12v1|BE495058
1032
492
492
100
globlastp

LAB633_H13
rye|12v1|BF145486
1033
492
492
100
globlastp

LAB633_H14
rye|12v1|DRR001012.178730
1034
492
492
100
globlastp

LAB633_H15
rye|12v1|DRR001013.134595
1035
492
492
100
globlastp

LAB633_H16
wheat|10v2|BE415525
1036
492
492
100
globlastp

LAB633_H16
wheat|12v3|BE405530_P1
1037
492
492
100
globlastp

LAB633_H84
wheat|12v3|BE400824_P1
1038
6401
492
99.4
globlastp

8

LAB633_H84
wheat|12v3|BE413605_P1
1039
6401
492
99.4
globlastp

9

LAB633_H85
wheat|12v3|CA637124_P1
1040
6401
492
99.4
globlastp

0

LAB633_H17
barley|10v2|BE413158
1041
6401
492
99.4
globlastp

LAB633_H17
barley|12v1|BE413158_P1
1042
6401
492
99.4
globlastp

LAB633_H18
brachypodium|12v1|BRADI2
1043
6402
492
99.4
globlastp

G39690_P1

LAB633_H19
brachypodium|12v1|BRADI4
1044
6401
492
99.4
globlastp

G24287_P1

LAB633_H20
brachypodium|12v1|BRADI4
1045
6401
492
99.4
globlastp

G41560_P1

LAB633_H21
cynodon|10v1|ES299096_P1
1046
6403
492
99.4
globlastp

LAB633_H22
cynodon|10v1|ES300110_P1
1047
6401
492
99.4
globlastp

LAB633_H23
maize|10v1|AI586913_P1
1048
6401
492
99.4
globlastp

LAB633_H24
maize|10v1|X92374_P1
1049
6401
492
99.4
globlastp

LAB633_H25
maize|10v1|X92375_P1
1050
6401
492
99.4
globlastp

LAB633_H26
millet|10v1|AF416606_P1
1051
6401
492
99.4
globlastp

LAB633_H27
millet|10v1|GFXAY620961X
1052
6401
492
99.4
globlastp

1_P1

LAB633_H28
pseudoroegneria|gb167|FF347
1053
6401
492
99.4
globlastp

054

LAB633_H29
rye|12v1|BF145534
1054
6401
492
99.4
globlastp

LAB633_H30
sorghum|12v1|SB05G004510
1055
6401
492
99.4
globlastp

LAB633_H31
sugarcane|10v1|BQ533789
1056
6401
492
99.4
globlastp

LAB633_H32
sugarcane|10v1|CA068850
1057
6401
492
99.4
globlastp

LAB633_H33
wheat|10v2|BE429139
1058
6401
492
99.4
globlastp

LAB633_H33
wheat|12v3|BE425161_P1
1059
6401
492
99.4
globlastp

LAB633_H34
wheat|10v2|CA484273
1060
6401
492
99.4
globlastp

LAB633_H34
wheat|12v3|CA484273_P1
1061
6401
492
99.4
globlastp

LAB633_H85
wheat|12v3|BE401163_P1
1062
6404
492
98.8
globlastp

1

LAB633_H35
foxtail_millet|11v3|EC613811
1063
6405
492
98.8
globlastp

_P1

LAB633_H36
lovegrass|gb167|DN481317_P
1064
6406
492
98.8
globlastp

1

LAB633_H37
rice|11v1|AA754273
1065
6407
492
98.8
globlastp

LAB633_H38
sorghum|12v1|SB08G004380
1066
6408
492
98.8
globlastp

LAB633_H39
sorghum|12v1|SB08G004390
1067
6408
492
98.8
globlastp

LAB633_H40
sugarcane|10v1|BQ533950
1068
6409
492
98.8
globlastp

LAB633_H41
wheat|10v2|AL819713
1069
6404
492
98.8
globlastp

LAB633_H41
wheat|12v3|BQ608651_P1
1070
6404
492
98.8
globlastp

LAB633_H42
wheat|10v2|BG313106
1071
6404
492
98.8
globlastp

LAB633_H42
wheat|12v3|BE426215_P1
1072
6404
492
98.8
globlastp

LAB633_H43
wheat|10v2|CA603994XX1
1073
6404
492
98.8
globlastp

LAB633_H43
wheat|12v3|BE404839_P1
1074
6404
492
98.8
globlastp

LAB633_H85
wheat|12v3|CA604013_T1
1075
6410
492
98.79
glotblastn

2

LAB633_H85
sesame|12v1|BU667591_P1
1076
6411
492
98.2
globlastp

3

LAB633_H44
basilicum|10v1|DY322167_P
1077
6411
492
98.2
globlastp

1

LAB633_H45
beech|11v1|SRR006293.1203
1078
6412
492
98.2
globlastp

_P1

LAB633_H46
beech|11v1|SRR006293.1714
1079
6412
492
98.2
globlastp

9_P1

LAB633_H47
beech|11v1|SRR006293.991_—
1080
6412
492
98.2
globlastp

P1

LAB633_H48
brachypodium|12v1|BRADI3
1081
6413
492
98.2
globlastp

G45420_P1

LAB633_H49
cenchrus|gb166|EB654899_P
1082
6414
492
98.2
globlastp

1

LAB633_H50
hornbeam|12v1|SRR364455.1
1083
6412
492
98.2
globlastp

03826_P1

LAB633_H51
maize|10v1|MZEORFD_P1
1084
6415
492
98.2
globlastp

LAB633_H52
oil_palm|11v1|EY409192_P1
1085
6416
492
98.2
globlastp

LAB633_H53
orobanche|10v1|SRR023189S
1086
6411
492
98.2
globlastp

0007505_P1

LAB633_H54
orobanche|10v1|SRR023189S
1087
6411
492
98.2
globlastp

0008087_P1

LAB633_H55
phyla|11v2|SRR099035X103
1088
6411
492
98.2
globlastp

952_P1

LAB633_H56
phyla|11v2|SRR099035X111
1089
6411
492
98.2
globlastp

19_P1

LAB633_H57
phyla|11v2|SRR099037X106
1090
6411
492
98.2
globlastp

816_P1

LAB633_H58
plantago|11v2|AM111326_P1
1091
6411
492
98.2
globlastp

LAB633_H59
plantago|11v2|SRR066373X1
1092
6411
492
98.2
globlastp

0076_P1

LAB633_H60
plantago|11v2|SRR066373X1
1093
6411
492
98.2
globlastp

01900_P1

LAB633_H61
plantago|11v2|SRR066373X1
1094
6411
492
98.2
globlastp

07481XX1_P1

LAB633_H62
plantago|11v2|SRR066373X1
1095
6411
492
98.2
globlastp

12910_P1

LAB633_H63
rice|11v1|OSU27098
1096
6417
492
98.2
globlastp

LAB633_H64
salvia|10v1|CV162426
1097
6411
492
98.2
globlastp

LAB633_H65
salvia|10v1|CV163575
1098
6411
492
98.2
globlastp

LAB633_H66
salvia|10v1|CV165970
1099
6411
492
98.2
globlastp

LAB633_H67
sesame|10v1|BU668441
1100
6411
492
98.2
globlastp

LAB633_H68
switchgrass|gb167|DN140816
1101
6418
492
98.2
globlastp

LAB633_H69
switchgrass|gb167|DN147486
1102
6418
492
98.2
globlastp

LAB633_H70
switchgrass|gb167|FL726205
1103
6418
492
98.2
globlastp

LAB633_H71
utricularia|11v1|SRR094438.1
1104
6411
492
98.2
globlastp

01758

LAB633_H72
utricularia|11v1|SRR094438.1
1105
6411
492
98.2
globlastp

0823

LAB633_H85
aquilegia|10v2|JGIAC003445
1106
6419
492
97.6
globlastp

4
_P1

LAB633_H85
aquilegia|10v2|JGIAC016860
1107
6419
492
97.6
globlastp

5
_P1

LAB633_H85
aquilegia|10v2|JGIAC016861
1108
6419
492
97.6
globlastp

6
_P1

LAB633_H85
blueberry|12v1|SRR353282X
1109
6420
492
97.6
globlastp

7
19997D1_P1

LAB633_H85
blueberry|12v1|SRR353282X
1110
6421
492
97.6
globlastp

8
48934D1_P1

LAB633_H85
blueberry|12v1|SRR353283X
1111
6420
492
97.6
globlastp

9
59210D1_P1

LAB633_H86
lettuce|12v1|DW047671_P1
1112
6419
492
97.6
globlastp

0

LAB633_H86
lettuce|12v1|DW049950_P1
1113
6419
492
97.6
globlastp

1

LAB633_H86
lettuce|12v1|DW052416_P1
1114
6419
492
97.6
globlastp

2

LAB633_H86
olea|13v1|SRR014463X12263
1115
6422
492
97.6
globlastp

3
D1_P1

LAB633_H86
olea|13v1|SRR014463X37052
1116
6419
492
97.6
globlastp

4
D1_P1

LAB633_H73
ambrosia|11v1|SRR346935.1
1117
6419
492
97.6
globlastp

36635_P1

LAB633_H74
ambrosia|11v1|SRR346943.1
1118
6419
492
97.6
globlastp

06432_P1

LAB633_H75
amorphophallus|11v2|SRR08
1119
6423
492
97.6
globlastp

9351X101594_P1

LAB633_H76
antirrhinum|gb166|AJ568930
1120
6424
492
97.6
globlastp

_P1

LAB633_H77
antirrhinum|gb166|AJ789388
1121
6424
492
97.6
globlastp

_P1

LAB633_H78
arnica|11v1|SRR099034X103
1122
6419
492
97.6
globlastp

156_P1

LAB633_H79
arnica|11v1|SRR099034X104
1123
6419
492
97.6
globlastp

823_P1

LAB633_H80
arnica|11v1|SRR099034X111
1124
6419
492
97.6
globlastp

168_P1

LAB633_H81
artemisia|10v1|EY034262_P1
1125
6419
492
97.6
globlastp

LAB633_H82
artemisia|10v1|EY034465_P1
1126
6419
492
97.6
globlastp

LAB633_H83
artemisia|10v1|EY034925_P1
1127
6419
492
97.6
globlastp

LAB633_H84
artemisia|10v1|EY041025_P1
1128
6419
492
97.6
globlastp

LAB633_H85
centaurea|gb166|EH732632_P
1129
6419
492
97.6
globlastp

1

LAB633_H86
centaurea|gb166|EL934361_P
1130
6419
492
97.6
globlastp

1

LAB633_H87
chelidonium|11v1|SRR08475
1131
6419
492
97.6
globlastp

2X100270_P1

LAB633_H88
chelidonium|11v1|SRR08475
1132
6419
492
97.6
globlastp

2X10178_P1

LAB633_H89
cichorium|gbl71|EH695839_—
1133
6419
492
97.6
globlastp

P1

LAB633_H90
cirsium|11v1|SRR346952.100
1134
6419
492
97.6
globlastp

1408_P1

LAB633_H91
cirsium|11v1|SRR346952.105
1135
6419
492
97.6
globlastp

930_P1

LAB633_H92
cirsium|11v1|SRR346952.114
1136
6419
492
97.6
globlastp

294_P1

LAB633_H93
cirsium|11v1|SRR346952.116
1137
6419
492
97.6
globlastp

592_P1

LAB633_H94
cleome_gynandra|10v1|SRR0
1138
6423
492
97.6
globlastp

15532S0004391_P1

LAB633_H95
cleome_gynandra|10v1|SRR0
1139
6423
492
97.6
globlastp

15532S0008445_P1

LAB633_H96
cleome_gynandra|10v1|SRR0
1140
6423
492
97.6
globlastp

15532S0015385_P1

LAB633_H97
cleome_gynandra|10v1|SRR0
1141
6423
492
97.6
globlastp

15532S0017364_P1

LAB633_H98
cleome_spinosa|10v1|GR9339
1142
6423
492
97.6
globlastp

79_P1

LAB633_H99
cleome_spinosa|10v1|SRR015
1143
6423
492
97.6
globlastp

531S0001748_P1

LAB633_H10
cleome_spinosa|10v1|SRR015
1144
6423
492
97.6
globlastp

0
531S0005669_P1

LAB633_H10
cucumber|09v1|AA660091_P
1145
6425
492
97.6
globlastp

1
1

LAB633_H10
cucumber|09v1|CK757080_P
1146
6426
492
97.6
globlastp

2
1

LAB633_H10
cucurbita|11v1|SRR091276X
1147
6419
492
97.6
globlastp

3
101055_P1

LAB633_H10
cucurbita|11v1|SRR091276X
1148
6419
492
97.6
globlastp

4
10385_P1

LAB633_H10
cucurbita|11v1|SRR091276X
1149
6419
492
97.6
globlastp

5
106496_P1

LAB633_H10
cucurbita|11v1|SRR091276X
1150
6419
492
97.6
globlastp

6
121934_P1

LAB633_H10
curcuma|10v1|DY391309_P1
1151
6423
492
97.6
globlastp

7

LAB633_H10
cynara|gb167|GE588641_P1
1152
6419
492
97.6
globlastp

8

LAB633_H10
cynara|gb167|GE589330_P1
1153
6419
492
97.6
globlastp

9

LAB633_H11
cynara|gb167|GE590889_P1
1154
6419
492
97.6
globlastp

0

LAB633_H11
dandelion|10v1|DR399179_P
1155
6419
492
97.6
globlastp

1
1

LAB633_H11
dandelion|10v1|DY813410_P
1156
6419
492
97.6
globlastp

2
1

LAB633_H11
eschscholzia|11v1|SRR01411
1157
6427
492
97.6
globlastp

3
6.103341_P1

LAB633_H11
eucalyptus|11v2|CD669362_P
1158
6419
492
97.6
globlastp

4
1

LAB633_H11
eucalyptus|11v2|CU403628_P
1159
6419
492
97.6
globlastp

5
1

LAB633_H11
euonymus|11v1|SRR070038X
1160
6419
492
97.6
globlastp

6
129055_P1

LAB633_H11
euonymus|11v1|SRR070038X
1161
6419
492
97.6
globlastp

7
19301_P1

LAB633_H11
euphorbia|11v1|DV138882_P
1162
6419
492
97.6
globlastp

8
1

LAB633_H11
flaveria|11v1|SRR149229.100
1163
6419
492
97.6
globlastp

9
132_P1

LAB633_H12
flaveria|11v1|SRR149229.101
1164
6419
492
97.6
globlastp

0
132_P1

LAB633_H12
flaveria|11v1|SRR149229.103
1165
6419
492
97.6
globlastp

1
382_P1

LAB633_H12
flaveria|11v1|SRR149229.105
1166
6419
492
97.6
globlastp

2
561_P1

LAB633_H12
flaveria|11v1|SRR149229.105
1167
6419
492
97.6
globlastp

3
836_P1

LAB633_H12
flaveria|11v1|SRR149229.108
1168
6419
492
97.6
globlastp

4
473_P1

LAB633_H12
flaveria|11v1|SRR149229.116
1169
6419
492
97.6
globlastp

5
585_P1

LAB633_H12
flaveria|11v1|SRR149229.138
1170
6419
492
97.6
globlastp

6
545_P1

LAB633_H12
flaveria|11v1|SRR149229.148
1171
6419
492
97.6
globlastp

7
499_P1

LAB633_H12
flaveria|11v1|SRR149229.206
1172
6419
492
97.6
globlastp

8
477XX2_P1

LAB633_H12
flaveria|11v1|SRR149232.117
1173
6419
492
97.6
globlastp

9
932_P1

LAB633_H13
flaveria|11v1|SRR149240.250
1174
6419
492
97.6
globlastp

0
2_P1

LAB633_H13
flaveria|11v1|SRR149241.101
1175
6419
492
97.6
globlastp

1
182_P1

LAB633_H13
flaveria|11v1|SRR149241.101
1176
6419
492
97.6
globlastp

2
373_P1

LAB633_H13
flaveria|11v1|SRR149241.105
1177
6419
492
97.6
globlastp

3
12_P1

LAB633_H13
flaveria|11v1|SRR149241.107
1178
6419
492
97.6
globlastp

4
333_P1

LAB633_H13
flaveria|11v1|SRR149244.160
1179
6419
492
97.6
globlastp

5
431_P1

LAB633_H13
foxtail_millet|11v3|PHY7SI0
1180
6428
492
97.6
globlastp

6
23455M_P1

LAB633_H13
fraxinus|11v1|SRR058827.10
1181
6425
492
97.6
globlastp

7
4108_P1

LAB633_H13
ginger|gb164|DY345311_P1
1182
6423
492
97.6
globlastp

8

LAB633_H13
ginger|gb164|DY347115_P1
1183
6423
492
97.6
globlastp

9

LAB633_H14
ginger|gb164|DY350603_P1
1184
6423
492
97.6
globlastp

0

LAB633_H14
ginger|gb164|DY354596_P1
1185
6423
492
97.6
globlastp

1

LAB633_H14
guizotia|10v1|GE561811_P1
1186
6419
492
97.6
globlastp

2

LAB633_H14
hornbeam|12v1|SRR364455.1
1187
6423
492
97.6
globlastp

3
35058_P1

LAB633_H14
kiwi|gb166|FG403438_P1
1188
6419
492
97.6
globlastp

4

LAB633_H14
kiwi|gb166|FG403625_P1
1189
6419
492
97.6
globlastp

5

LAB633_H14
kiwi|gb166|FG404684_P1
1190
6419
492
97.6
globlastp

6

LAB633_H14
kiwi|gb166|FG408209_P1
1191
6419
492
97.6
globlastp

7

LAB633_H14
kiwi|gb166|FG409549_P1
1192
6419
492
97.6
globlastp

8

LAB633_H14
kiwi|gb166|FG431746_P1
1193
6419
492
97.6
globlastp

9

LAB633_H15
kiwi|gb166|FG482106_P1
1194
6419
492
97.6
globlastp

0

LAB633_H15
lettuce|10v1|DW047671
1195
6419
492
97.6
globlastp

1

LAB633_H15
lettuce|10v1|DW049950
1196
6419
492
97.6
globlastp

2

LAB633_H15
lettuce|10v1|DW052416
1197
6419
492
97.6
globlastp

3

LAB633_H15
lettuce|10v1|DW076667
1198
6419
492
97.6
globlastp

4

LAB633_H15
liriodendron|gb166|CK74795
1199
6419
492
97.6
globlastp

5
4_P1

LAB633_H15
liriodendron|gb166|CK76523
1200
6419
492
97.6
globlastp

6
4_P1

LAB633_H15
maize|10v1|T70653_P1
1201
6429
492
97.6
globlastp

7

LAB633_H15
melon|10v1|AM716244_P1
1202
6419
492
97.6
globlastp

8

LAB633_H15
melon|10v1|EB715750_P1
1203
6419
492
97.6
globlastp

9

LAB633_H16
momordica|10v1|SRR071315
1204
6419
492
97.6
globlastp

0
S0012856_P1

LAB633_H16
monkeyflower|10v1|DV20888
1205
6430
492
97.6
globlastp

1
3

LAB633_H16
monkeyflower|12v1|DV20888
1206
6430
492
97.6
globlastp

1
3_P1

LAB633_H16
monkeyflower|10v1|DV20937
1207
6430
492
97.6
globlastp

2
9

LAB633_H16
monkeyflower|12v1|DV20937
1208
6430
492
97.6
globlastp

2
9_P1

LAB633_H16
monkeyflower|10v1|DV20979
1209
6430
492
97.6
globlastp

3
7

LAB633_H16
monkeyflower|12v1|DV20979
1210
6430
492
97.6
globlastp

3
7_P1

LAB633_H16
oat|11v1|GO583800_P1
1211
6431
492
97.6
globlastp

4

LAB633_H16
oil_palm|11v1|ES370775_P1
1212
6423
492
97.6
globlastp

5

LAB633_H16
olea|11v1|SRR014463.13485
1213
6419
492
97.6
globlastp

6

LAB633_H16
olea|13v1|SRR014463X13485
1214
6419
492
97.6
globlastp

6
D1_P1

LAB633_H16
orange|11v1|AB024274_P1
1215
6425
492
97.6
globlastp

7

LAB633_H16
orange|11v1|CB290639_P1
1216
6426
492
97.6
globlastp

8

LAB633_H16
orobanche|10v1|SRR023189S
1217
6430
492
97.6
globlastp

9
0000251_P1

LAB633_H17
orobanche|10v1|SRR023189S
1218
6430
492
97.6
globlastp

0
0083703_P1

LAB633_H17
parthenium|10v1|GW775598_—
1219
6419
492
97.6
globlastp

1
P1

LAB633_H17
parthenium|10v1|GW778894_—
1220
6419
492
97.6
globlastp

2
P1

LAB633_H17
parthenium|10v1|GW781801_—
1221
6419
492
97.6
globlastp

3
P1

LAB633_H17
phyla|11v2|SRR099035X102
1222
6432
492
97.6
globlastp

4
79_P1

LAB633_H17
poppy|11v1|FE965497_P1
1223
6419
492
97.6
globlastp

5

LAB633_H17
poppy|11v1|FE967925_P1
1224
6419
492
97.6
globlastp

6

LAB633_H17
poppy|11v1|SRR030259.1017
1225
6426
492
97.6
globlastp

7
06_P1

LAB633_H17
poppy|11v1|SRR030259.1019
1226
6426
492
97.6
globlastp

8
39_P1

LAB633_H17
poppy|11v1|SRR096789.1040
1227
6419
492
97.6
globlastp

9
22_P1

LAB633_H18
poppy|11v1|SRR096789.1048
1228
6419
492
97.6
globlastp

0
33_P1

LAB633_H18
rice|11v1|BE040702
1229
6433
492
97.6
globlastp

1

LAB633_H18
rose|12v1|BI977379
1230
6419
492
97.6
globlastp

2

LAB633_H18
rye|12v1|BF145399
1231
6434
492
97.6
globlastp

3

LAB633_H18
rye|12v1|DRR001012.150829
1232
6434
492
97.6
globlastp

4

LAB633_H18
safflower|gb162|EL398959
1233
6419
492
97.6
globlastp

5

LAB633_H18
safflower|gb162|EL400023
1234
6419
492
97.6
globlastp

6

LAB633_H18
safflower|gb162|EL401162
1235
6419
492
97.6
globlastp

7

LAB633_H18
safflower|gb162|EL402112
1236
6419
492
97.6
globlastp

8

LAB633_H18
sarracenia|11v1|SRR192669.1
1237
6419
492
97.6
globlastp

9
01330

LAB633_H19
sarracenia|11v1|SRR192669.1
1238
6419
492
97.6
globlastp

0
03204

LAB633_H19
sarracenia|11v1|SRR192669.1
1239
6419
492
97.6
globlastp

1
04490

LAB633_H19
scabiosa|11v1|SRR063723X1
1240
6419
492
97.6
globlastp

2
00330

LAB633_H19
scabiosa|11v1|SRR063723X1
1241
6419
492
97.6
globlastp

3
00627

LAB633_H19
scabiosa|11v1|SRR063723X1
1242
6419
492
97.6
globlastp

4
00798

LAB633_H19
scabiosa|11v1|SRR063723X1
1243
6419
492
97.6
globlastp

5
02228

LAB633_H19
scabiosa|11v1|SRR063723X1
1244
6419
492
97.6
globlastp

6
11751

LAB633_H19
senecio|gb170|DY661370
1245
6419
492
97.6
globlastp

7

LAB633_H19
sunflower|12v1|CD845624
1246
6419
492
97.6
globlastp

8

LAB633_H19
sunflower|12v1|DY910343
1247
6419
492
97.6
globlastp

9

LAB633_H20
sunflower|12v1|DY914673
1248
6419
492
97.6
globlastp

0

LAB633_H20
sunflower|12v1|DY917358
1249
6419
492
97.6
globlastp

1

LAB633_H20
sunflower|12v1|DY943926
1250
6419
492
97.6
globlastp

2

LAB633_H20
sunflower|12v1|EE638178
1251
6419
492
97.6
globlastp

3

LAB633_H20
switchgrass|12v1|DN142552_—
1252
6435
492
97.6
globlastp

4
P1

LAB633_H20
switchgrass|gb167|DN142552
1253
6435
492
97.6
globlastp

4

LAB633_H20
tragopogon|10v1|SRR020205
1254
6419
492
97.6
globlastp

5
S0003683

LAB633_H20
tragopogon|10v1|SRR020205
1255
6419
492
97.6
globlastp

6
S0032939

LAB633_H20
triphysaria|10v1|DR173974
1256
6430
492
97.6
globlastp

7

LAB633_H20
triphysaria|10v1|EY000502
1257
6430
492
97.6
globlastp

8

LAB633_H20
triphysaria|10v1|EY127066
1258
6430
492
97.6
globlastp

9

LAB633_H21
triphysaria|10v1|EY128086
1259
6430
492
97.6
globlastp

0

LAB633_H21
triphysaria|10v1|EY131693
1260
6430
492
97.6
globlastp

1

LAB633_H21
triphysaria|10v1|EY133280
1261
6430
492
97.6
globlastp

2

LAB633_H21
utricularia|11v1|SRR094438.1
1262
6424
492
97.6
globlastp

3
00321

LAB633_H21
valeriana|11v1|SRR099039X1
1263
6419
492
97.6
globlastp

4
01962

LAB633_H21
valeriana|11v1|SRR099039X1
1264
6419
492
97.6
globlastp

5
04138

LAB633_H21
vinca|11v1|SRR098690X1011
1265
6436
492
97.6
globlastp

6
83

LAB633_H21
vinca|11v1|SRR098690X1032
1266
6436
492
97.6
globlastp

7
98

LAB633_H21
vinca|11v1|SRR098690X1151
1267
6436
492
97.6
globlastp

8
04

LAB633_H21
watermelon|11v1|AA660091
1268
6419
492
97.6
globlastp

9

LAB633_H22
watermelon|11v1|CK757080
1269
6419
492
97.6
globlastp

0

LAB633_H22
wheat|10v2|CA485794
1270
6433
492
97.6
globlastp

1

LAB633_H22
ambrosia|11v1|SRR346935.1
1271
6437
492
97.58
glotblastn

2
04209_T1

LAB633_H86
aquilegia|10v2|JGIAC011264
1272
6438
492
97
globlastp

5
_P1

LAB633_H86
bean|12v2|CA901989_P1
1273
6439
492
97
globlastp

6

LAB633_H86
blueberry|12v1|SRR353282X
1274
6440
492
97
globlastp

7
101071D1_P1

LAB633_H86
blueberry|12v1|SRR353282X
1275
6441
492
97
globlastp

8
24384D1_P1

LAB633_H86
olea|13v1|SRR014463X21059
1276
6442
492
97
globlastp

9
D1_P1

LAB633_H87
zostera|12v1|SRR057351X10
1277
6443
492
97
globlastp

0
3945D1_P1

LAB633_H22
amsonia|11v1|SRR098688X1
1278
6444
492
97
globlastp

3
02958_P1

LAB633_H22
amsonia|11v1|SRR098688X1
1279
6445
492
97
globlastp

4
03733_P1

LAB633_H22
antirrhinum|gb166|AJ788617
1280
6446
492
97
globlastp

5
_P1

LAB633_H22
antirrhinum|gb166|AJ802464
1281
6447
492
97
globlastp

6
_P1

LAB633_H22
apple|11v1|CN878272_P1
1282
6448
492
97
globlastp

7

LAB633_H22
aristolochia|10v1|FD751918_—
1283
6449
492
97
globlastp

8
P1

LAB633_H22
arnica|11v1|SRR099034X119
1284
6450
492
97
globlastp

9
562_P1

LAB633_H23
bean|12v1|CA901989
1285
6439
492
97
globlastp

0

LAB633_H23
beech|11v1|AM062973_P1
1286
6451
492
97
globlastp

1

LAB633_H23
beech|11v1|SRR006293.3038
1287
6451
492
97
globlastp

2
3_P1

LAB633_H23
beet|12v1|AJ002061_P1
1288
6452
492
97
globlastp

3

LAB633_H23
beet|12v1|X98851_P1
1289
6452
492
97
globlastp

4

LAB633_H23
bupleurum|11v1|FG341924_P
1290
6453
492
97
globlastp

5
1

LAB633_H23
cacao|10v1|CA794658_P1
1291
6454
492
97
globlastp

6

LAB633_H23
cacao|10v1|CA795252_P1
1292
6454
492
97
globlastp

7

LAB633_H23
cacao|10v1|CU476305_P1
1293
6454
492
97
globlastp

8

LAB633_H23
cacao|10v1|CU481444_P1
1294
6454
492
97
globlastp

9

LAB633_H24
cannabis|12v1|EW701787_P1
1295
6454
492
97
globlastp

0

LAB633_H24
cannabis|12v1|SOLX0006324
1296
6454
492
97
globlastp

1
5_P1

LAB633_H24
cassava|09v1|CK648184_P1
1297
6455
492
97
globlastp

2

LAB633_H24
cassava|09v1|FF534471_P1
1298
6455
492
97
globlastp

3

LAB633_H24
castorbean|12v1|EE258463_P
1299
6455
492
97
globlastp

4
1

LAB633_H24
castorbean|12v1|EG657815_P
1300
6455
492
97
globlastp

5
1

LAB633_H24
castorbean|11v1|EG698149
1301
6455
492
97
globlastp

6

LAB633_H24
castorbean|12v1|EG698149_P
1302
6455
492
97
globlastp

6
1

LAB633_H24
catharanthus|11v1|EG555852
1303
6456
492
97
globlastp

7
_P1

LAB633_H24
catharanthus|11v1|EG556119
1304
6444
492
97
globlastp

8
_P1

LAB633_H24
centaurea|gb166|EH727314_P
1305
6457
492
97
globlastp

9
1

LAB633_H25
cichorium|gb171|EH690425_—
1306
6458
492
97
globlastp

0
P1

LAB633_H25
cichorium|gbl71|EH698654_—
1307
6450
492
97
globlastp

1
P1

LAB633_H25
clementine|11v1|AB024274_—
1308
6459
492
97
globlastp

2
P1

LAB633_H25
clementine|11v1|AB024275_—
1309
6454
492
97
globlastp

3
P1

LAB633_H25
clementine|11v1|AB024276_—
1310
6460
492
97
globlastp

4
P1

LAB633_H25
clementine|11v1|CB290639_P
1311
6461
492
97
globlastp

5
1

LAB633_H25
cleome_spinosa|10v1|GR9331
1312
6462
492
97
globlastp

6
75_P1

LAB633_H25
cleome_spinosa|10v1|SRR015
1313
6463
492
97
globlastp

7
531S0024217_P1

LAB633_H25
cleome_spinosa|10v1|SRR015
1314
6463
492
97
globlastp

8
531S0031567_P1

LAB633_H25
coffea|10v1|DV664078_P1
1315
6444
492
97
globlastp

9

LAB633_H26
coffea|10v1|DV672722_P1
1316
6444
492
97
globlastp

0

LAB633_H26
coffea|10v1|DV677102_P1
1317
6444
492
97
globlastp

1

LAB633_H26
cotton|11v1|AI727227_P1
1318
6454
492
97
globlastp

2

LAB633_H26
cotton|11v1|CO496515_P1
1319
6454
492
97
globlastp

3

LAB633_H26
cotton|11v1|GHU13669_P1
1320
6454
492
97
globlastp

4

LAB633_H26
cotton|11v1|SRR032367.1066
1321
6454
492
97
globlastp

5
65_P1

LAB633_H26
cowpea|12v1|FF537329_P1
1322
6439
492
97
globlastp

6

LAB633_H26
cowpea|gb166|FF537329
1323
6439
492
97
globlastp

6

LAB633_H26
cucumber|09v1|AM716244_P
1324
6464
492
97
globlastp

7
1

LAB633_H26
cucurbita|11v1|SRR091276X
1325
6465
492
97
globlastp

8
112115_P1

LAB633_H26
cucurbita|11v1|SRR091276X
1326
6465
492
97
globlastp

9
115189_P1

LAB633_H27
dandelion|10v1|DR399923_P
1327
6461
492
97
globlastp

0
1

LAB633_H27
epimedium|11v1|SRR013502.
1328
6438
492
97
globlastp

1
25475_P1

LAB633_H27
euonymus|11v1|SRR070038X
1329
6466
492
97
globlastp

2
107842_P1

LAB633_H27
euonymus|11v1|SRR070038X
1330
6465
492
97
globlastp

3
128865_P1

LAB633_H27
euonymus|11v1|SRR070038X
1331
6465
492
97
globlastp

4
132672_P1

LAB633_H27
flaveria|11v1|SRR149232.102
1332
6467
492
97
globlastp

5
632_P1

LAB633_H27
flaveria|11v1|SRR149232.111
1333
6454
492
97
globlastp

6
262_P1

LAB633_H27
flax|11v1|CV478820_P1
1334
6465
492
97
globlastp

7

LAB633_H27
flax|11v1|CV478915_P1
1335
6465
492
97
globlastp

8

LAB633_H27
flax|11v1|EU829089_P1
1336
6465
492
97
globlastp

9

LAB633_H28
flax|11v1|GW865805_P1
1337
6465
492
97
globlastp

0

LAB633_H28
flax|11v1|JG022116_P1
1338
6468
492
97
globlastp

1

LAB633_H28
flax|11v1|JG082027_P1
1339
6465
492
97
globlastp

2

LAB633_H28
foxtail_millet|11v3|PHY7SI0
1340
6469
492
97
globlastp

3
12647M_P1

LAB633_H28
fraxinus|11v1|SRR058827.12
1341
6470
492
97
globlastp

4
3841_P1

LAB633_H28
gerbera|09v1|AJ751180_P1
1342
6471
492
97
globlastp

5

LAB633_H28
gossypium_raimondii|12v1|E
1343
6454
492
97
globlastp

6
S826736_P1

LAB633_H28
gossypium_raimondii|12v1|G
1344
6454
492
97
globlastp

7
HU13669_P1

LAB633_H28
guizotia|10v1|GE557183_P1
1345
6472
492
97
globlastp

8

LAB633_H28
hevea|10v1|EC600159_P1
1346
6455
492
97
globlastp

9

LAB633_H29
humulus|11v1|ES654825_P1
1347
6454
492
97
globlastp

0

LAB633_H29
humulus|11v1|ES654966_P1
1348
6454
492
97
globlastp

1

LAB633_H29
humulus|11v1|EX515795_P1
1349
6454
492
97
globlastp

2

LAB633_H29
jatropha|09v1|GH295935_P1
1350
6473
492
97
globlastp

3

LAB633_H29
lettuce|10v1|DW083002
1351
6474
492
97
globlastp

4

LAB633_H29
liriodendron|gb166|CK75148
1352
6438
492
97
globlastp

5
6_P1

LAB633_H29
maritime_pine|10v1|BX24944
1353
6475
492
97
globlastp

6
3_P1

LAB633_H29
millet|10v1|CD726180_P1
1354
6469
492
97
globlastp

7

LAB633_H29
millet|10v1|EVO454PM0054
1355
6469
492
97
globlastp

8
59_P1

LAB633_H29
momordica|10v1|SRR071315
1356
6476
492
97
globlastp

9
S0002019_P1

LAB633_H30
nuphar|gb166|CK751168_P1
1357
6477
492
97
globlastp

0

LAB633_H30
oil_palm|11v1|DW248073_P1
1358
6478
492
97
globlastp

1

LAB633_H30
oil_palm|11v1|EL683839_P1
1359
6478
492
97
globlastp

2

LAB633_H30
oil_palm|11v1|EL685221_P1
1360
6479
492
97
globlastp

3

LAB633_H30
oil_palm|11v1|SRR190698.25
1361
6478
492
97
globlastp

4
4295_P1

LAB633_H30
orange|11v1|AB024275_P1
1362
6454
492
97
globlastp

5

LAB633_H30
orange|11v1|AB024276_P1
1363
6460
492
97
globlastp

6

LAB633_H30
papaya|gb165|EX258745_P1
1364
6480
492
97
globlastp

7

LAB633_H30
pine|10v2|AI812928_P1
1365
6475
492
97
globlastp

8

LAB633_H30
poplar|10v1|BI121380
1366
6454
492
97
globlastp

9

LAB633_H30
poplar|13v1|BI121380_P1
1367
6454
492
97
globlastp

9

LAB633_H31
primula|11v1|SRR098679X10
1368
6481
492
97
globlastp

0
0571_P1

LAB633_H31
pteridium|11v1|SRR043594X
1369
6454
492
97
globlastp

1
121574

LAB633_H31
rice|11v1|BI806048
1370
6482
492
97
globlastp

2

LAB633_H31
rose|12v1|SRR397984.10825
1371
6483
492
97
globlastp

3
1

LAB633_H31
sarracenia|11v1|SRR192669.1
1372
6484
492
97
globlastp

4
05728

LAB633_H31
sarracenia|11v1|SRR192669.1
1373
6485
492
97
globlastp

5
34358

LAB633_H31
spurge|gb161|DV138882
1374
6486
492
97
globlastp

6

LAB633_H31
sugarcane|10v1|CA090188
1375
6487
492
97
globlastp

7

LAB633_H31
sunflower|12v1|CF076229
1376
6488
492
97
globlastp

8

LAB633_H31
switchgrass|12v1|DN152313_—
1377
6489
492
97
globlastp

9
P1

LAB633_H31
switchgrass|gb167|DN152313
1378
6489
492
97
globlastp

9

LAB633_H32
tabernaemontana|11v1|SRR09
1379
6444
492
97
globlastp

0
8689X101164

LAB633_H32
tabernaemontana|11v1|SRR09
1380
6444
492
97
globlastp

1
8689X102587

LAB633_H32
tabernaemontana|11v1|SRR09
1381
6456
492
97
globlastp

2
8689X113197

LAB633_H32
tamarix|gb166|EG970753
1382
6490
492
97
globlastp

3

LAB633_H32
tea|10v1|CV013991
1383
6465
492
97
globlastp

4

LAB633_H32
tea|10v1|GE650473
1384
6465
492
97
globlastp

5

LAB633_H32
thellungiella_halophilum|11v
1385
6481
492
97
globlastp

6
1|DN773957

LAB633_H32
thellungiella_parvulum|11v1|
1386
6481
492
97
globlastp

7
DN773957

LAB633_H32
tripterygium|11v1|SRR09867
1387
6454
492
97
globlastp

8
7X100203

LAB633_H32
tripterygium|11v1|SRR09867
1388
6454
492
97
globlastp

9
7X102749

LAB633_H33
tripterygium|11v1|SRR09867
1389
6454
492
97
globlastp

0
7X105712

LAB633_H33
tripterygium|11v1|SRR09867
1390
6465
492
97
globlastp

1
7X115599

LAB633_H33
valeriana|11v1|SRR099039X1
1391
6438
492
97
globlastp

2
10443

LAB633_H33
vinca|11v1|SRR098690X1064
1392
6491
492
97
globlastp

3
16

LAB633_H33
vinca|11v1|SRR098690X1342
1393
6444
492
97
globlastp

4
72

LAB633_H33
watermelon|11v1|SRR071315
1394
6465
492
97
globlastp

5
.123428

LAB633_H87
blueberry|12v1|DR067871_T
1395
6492
492
96.97
glotblastn

1
1

LAB633_H33
amorphophallus|11v2|SRR08
1396
6493
492
96.97
glotblastn

6
9351X118783_T1

LAB633_H33
flaveria|11v1|SRR149232.152
1397
6494
492
96.97
glotblastn

7
046_T1

LAB633_H33
sarracenia|11v1|SRR192669.1
1398
6495
492
96.97
glotblastn

8
17471

LAB633_H33
thalictrum|11v1|SRR096787X
1399
6496
492
96.97
glotblastn

9
163886

LAB633_H34
zostera|10v1|AM766942
1400
6497
492
96.97
glotblastn

0

LAB633_H68,
switchgrass|12v1|DN147486_—
1401
6498
492
96.97
glotblastn

LAB633_H69
T1

LAB633_H87
b_juncea|12v1|E6ANDIZ01A
1402
6499
492
96.4
globlastp

2
R9Y5_P1

LAB633_H87
banana|12v1|BBS3072T3_P1
1403
6500
492
96.4
globlastp

3

LAB633_H87
banana|12v1|ES434616_P1
1404
6501
492
96.4
globlastp

4

LAB633_H87
banana|12v1|FL658132_P1
1405
6500
492
96.4
globlastp

5

LAB633_H87
banana|12v1|MAGEN201203
1406
6500
492
96.4
globlastp

6
6094_P1

LAB633_H87
bean|12v2|CA901986_P1
1407
6502
492
96.4
globlastp

7

LAB633_H87
bean|12v2|SRR001334.11668
1408
6502
492
96.4
globlastp

8
9_P1

LAB633_H87
nicotiana_benthamiana|12v1|
1409
6503
492
96.4
globlastp

9
CN744250_P1

LAB633_H88
nicotiana_benthamiana|12v1|
1410
6503
492
96.4
globlastp

0
EB428533_P1

LAB633_H88
olea|13v1|SRR014464X40253
1411
6504
492
96.4
globlastp

1
D1_P1

LAB633_H88
onion|12v1|CF439201_P1
1412
6505
492
96.4
globlastp

2

LAB633_H88
onion|12v1|SRR073446X104
1413
6502
492
96.4
globlastp

3
846D1_P1

LAB633_H88
zostera|12v1|AM408842_P1
1414
6506
492
96.4
globlastp

4

LAB633_H88
zostera|12v1|SRR057351X10
1415
6506
492
96.4
globlastp

5
1597D1_P1

LAB633_H88
zostera|12v1|SRR287819X33
1416
6506
492
96.4
globlastp

6
829D1_P1

LAB633_H34
abies|11v2|SRR098676X1145
1417
6507
492
96.4
globlastp

1
77_P1

LAB633_H34
amborella|12v2|CK764243
1418
6508
492
96.4
globlastp

2

LAB633_H34
amorphophallus|11v2|SRR08
1419
6500
492
96.4
globlastp

3
9351X100291_P1

LAB633_H34
amorphophallus|11v2|SRR08
1420
6500
492
96.4
globlastp

4
9351X124783_P1

LAB633_H34
amorphophallus|11v2|SRR08
1421
6500
492
96.4
globlastp

5
9351X198671_P1

LAB633_H34
amsonia|11v1|SRR098688X1
1422
6509
492
96.4
globlastp

6
00051_P1

LAB633_H34
arabidopsis_lyrata|09v1|JGIA
1423
6510
492
96.4
globlastp

7
L002096_P1

LAB633_H34
arabidopsis|10v1|ATlG19910
1424
6511
492
96.4
globlastp

8
_P1

LAB633_H34
avocado|10v1|CK751989_P1
1425
6512
492
96.4
globlastp

9

LAB633_H35
b_oleracea|gb161|DY026088_—
1426
6499
492
96.4
globlastp

0
P1

LAB633_H35
b_oleracea|gb161|DY026959_—
1427
6499
492
96.4
globlastp

1
P1

LAB633_H35
b_rapa|11v1|L37488_P1
1428
6499
492
96.4
globlastp

2

LAB633_H35
banana|10v1|FL658132
1429
6500
492
96.4
globlastp

3

LAB633_H35
bean|12v1|CA901986
1430
6502
492
96.4
globlastp

4

LAB633_H35
bean|12v1|SRR001334.11668
1431
6502
492
96.4
globlastp

5
9

LAB633_H35
canola|11v1|CN728789_P1
1432
6499
492
96.4
globlastp

6

LAB633_H35
canola|11v1|DY007332_P1
1433
6499
492
96.4
globlastp

7

LAB633_H35
canola|11v1|EE424868_P1
1434
6499
492
96.4
globlastp

8

LAB633_H35
canola|11v1|EE458205_P1
1435
6499
492
96.4
globlastp

9

LAB633_H36
canola|11v1|EE458505_P1
1436
6499
492
96.4
globlastp

0

LAB633_H36
canola|11v1|EE460051_P1
1437
6499
492
96.4
globlastp

1

LAB633_H36
canola|11v1|EE460054_P1
1438
6499
492
96.4
globlastp

2

LAB633_H36
canola|11v1|EG020714_P1
1439
6513
492
96.4
globlastp

3

LAB633_H36
cassava|09v1|CK642847_P1
1440
6514
492
96.4
globlastp

4

LAB633_H36
cassava|09v1|CK648677_P1
1441
6515
492
96.4
globlastp

5

LAB633_H36
cassava|09v1|CK649863_P1
1442
6499
492
96.4
globlastp

6

LAB633_H36
cassava|09v1|DR085043_P1
1443
6499
492
96.4
globlastp

7

LAB633_H36
cassava|09v1|DV456713_P1
1444
6511
492
96.4
globlastp

8

LAB633_H36
chelidonium|11v1|SRR08475
1445
6500
492
96.4
globlastp

9
2X104569_P1

LAB633_H37
chestnut|gb170|SRR006295S0
1446
6516
492
96.4
globlastp

0
041423_P1

LAB633_H37
cichorium|gb171|EH701723_—
1447
6517
492
96.4
globlastp

1
P1

LAB633_H37
cirsium|11v1|SRR346952.101
1448
6518
492
96.4
globlastp

2
1193_P1

LAB633_H37
cleome_gynandra|10v1|SRR0
1449
6499
492
96.4
globlastp

3
15532S0017877_P1

LAB633_H37
cleome_spinosa|10v1|SRR015
1450
6519
492
96.4
globlastp

4
531S0001162_P1

LAB633_H37
cotton|11v1|AF064202_P1
1451
6511
492
96.4
globlastp

5

LAB633_H37
cotton|11v1|AI054952_P1
1452
6511
492
96.4
globlastp

6

LAB633_H37
cotton|11v1|AI725776_P1
1453
6511
492
96.4
globlastp

7

LAB633_H37
cotton|11v1|BF272348_P1
1454
6511
492
96.4
globlastp

8

LAB633_H37
cotton|11v1|BQ405626_P1
1455
6511
492
96.4
globlastp

9

LAB633_H38
cotton|11v1|CO094873_P1
1456
6511
492
96.4
globlastp

0

LAB633_H38
cotton|11v1|DR454100_P1
1457
6511
492
96.4
globlastp

1

LAB633_H38
cotton|11v1|GHU13670_P1
1458
6520
492
96.4
globlastp

2

LAB633_H38
cowpea|12v1|FC458341_P1
1459
6502
492
96.4
globlastp

3

LAB633_H38
cowpea|gb166|FC458341
1460
6502
492
96.4
globlastp

3

LAB633_H38
cowpea|12v1|FF403369_P1
1461
6502
492
96.4
globlastp

4

LAB633_H38
cowpea|gb166|FF403369
1462
6502
492
96.4
globlastp

4

LAB633_H38
cycas|gb166|CB091951_P1
1463
6521
492
96.4
globlastp

5

LAB633_H38
eschscholzia|11v1|CD480350
1464
6522
492
96.4
globlastp

6
_P1

LAB633_H38
eschscholzia|11v1|CK749635
1465
6523
492
96.4
globlastp

7
_P1

LAB633_H38
eschscholzia|11v1|SRR01411
1466
6522
492
96.4
globlastp

8
6.102395_P1

LAB633_H38
eschscholzia|11v1|SRR01411
1467
6522
492
96.4
globlastp

9
6.126067_P1

LAB633_H39
eucalyptus|11v2|CD668139_P
1468
6500
492
96.4
globlastp

0
1

LAB633_H39
euphorbia|11v1|BP958527_P1
1469
6524
492
96.4
globlastp

1

LAB633_H39
flaveria|11v1|SRR149229.104
1470
6525
492
96.4
globlastp

2
035_P1

LAB633_H39
flaveria|11v1|SRR149232.100
1471
6525
492
96.4
globlastp

3
621_P1

LAB633_H39
fraxinus|11v1|SRR058827.11
1472
6504
492
96.4
globlastp

4
1126_P1

LAB633_H39
gerbera|09v1|AJ750461_P1
1473
6526
492
96.4
globlastp

5

LAB633_H39
gossypium_raimondii|12v1|A
1474
6511
492
96.4
globlastp

6
F064202_P1

LAB633_H39
gossypium_raimondii|12v1|AI
1475
6511
492
96.4
globlastp

7
054952_P1

LAB633_H39
gossypium_raimondii|12v1|B
1476
6511
492
96.4
globlastp

8
F277739_P1

LAB633_H39
gossypium_raimondii|12v1|B
1477
6511
492
96.4
globlastp

9
Q405626_P1

LAB633_H40
iceplant|gb164|BE034027_P1
1478
6527
492
96.4
globlastp

0

LAB633_H40
iceplant|gb164|BE036727_P1
1479
6528
492
96.4
globlastp

1

LAB633_H40
ipomoea_batatas|10v1|BU690
1480
6529
492
96.4
globlastp

2
365_P1

LAB633_H40
ipomoea_batatas|10v1|DV036
1481
6530
492
96.4
globlastp

3
354_P1

LAB633_H40
ipomoea_batatas|10v1|EE878
1482
6529
492
96.4
globlastp

4
392_P1

LAB633_H40
ipomoea_nil|10v1|BJ553380_—
1483
6503
492
96.4
globlastp

5
P1

LAB633_H40
ipomoea_nil|10v1|BJ554757_—
1484
6503
492
96.4
globlastp

6
P1

LAB633_H40
kiwi|gb166|FG405089_P1
1485
6500
492
96.4
globlastp

7

LAB633_H40
kiwi|gb166|FG445404_P1
1486
6531
492
96.4
globlastp

8

LAB633_H40
liquorice|gbl71|FS255772_P1
1487
6502
492
96.4
globlastp

9

LAB633_H41
nasturtium|11v1|SRR032558.
1488
6532
492
96.4
globlastp

0
108757_P1

LAB633_H41
nasturtium|11v1|SRR032559.
1489
6533
492
96.4
globlastp

1
155247_P1

LAB633_H41
nicotiana_benthamiana|12v1|
1490
6503
492
96.4
globlastp

2
X95751_P1

LAB633_H41
nicotiana_benthamiana|gb162|
1491
6503
492
96.4
globlastp

2
CN655207

LAB633_H41
nicotiana_benthamiana|gb162|
1492
6503
492
96.4
globlastp

3
CN742495

LAB633_H41
oil_palm|11v1|EL689034_P1
1493
6534
492
96.4
globlastp

4

LAB633_H41
olea|11v1|SRR014463.10832
1494
6504
492
96.4
globlastp

5

LAB633_H41
olea|13v1|SRR014463X10832
1495
6504
492
96.4
globlastp

5
D1_P1

LAB633_H41
olea|11v1|SRR014463.12263
1496
6535
492
96.4
globlastp

6

LAB633_H41
olea|11v1|SRR014463.18790
1497
6504
492
96.4
globlastp

7

LAB633_H41
olea|13v1|SRR014463X18790
1498
6504
492
96.4
globlastp

7
D1_P1

LAB633_H41
onion|gb162|CF439201
1499
6505
492
96.4
globlastp

8

LAB633_H41
papaya|gb165|AM904195_P1
1500
6499
492
96.4
globlastp

9

LAB633_H42
papaya|gb165|AM904284_P1
1501
6536
492
96.4
globlastp

0

LAB633_H42
phalaenopsis|11v1|CB032114
1502
6502
492
96.4
globlastp

1
_P1

LAB633_H42
phalaenopsis|11v1|SRR12577
1503
6502
492
96.4
globlastp

2
1.100193_P1

LAB633_H42
phalaenopsis|11v1|SRR12577
1504
6502
492
96.4
globlastp

3
1.1002777_P1

LAB633_H42
pigeonpea|11v1|SRR054580X
1505
6502
492
96.4
globlastp

4
126830_P1

LAB633_H42
pigeonpea|11v1|SRR054580X
1506
6502
492
96.4
globlastp

5
181428_P1

LAB633_H42
platanus|11v1|SRR096786X1
1507
6500
492
96.4
globlastp

6
01770_P1

LAB633_H42
platanus|11v1|SRR096786X1
1508
6500
492
96.4
globlastp

7
0393_P1

LAB633_H42
platanus|11v1|SRR096786X1
1509
6500
492
96.4
globlastp

8
07761_P1

LAB633_H42
platanus|11v1|SRR096786X1
1510
6500
492
96.4
globlastp

9
34140_P1

LAB633_H43
poplar|10v1|AI165129
1511
6537
492
96.4
globlastp

0

LAB633_H43
poplar|13v1|AI165129_P1
1512
6511
492
96.4
globlastp

0

LAB633_H43
poplar|10v1|BI128908
1513
6538
492
96.4
globlastp

1

LAB633_H43
poplar|13v1|BI128908_P1
1514
6538
492
96.4
globlastp

1

LAB633_H43
poplar|10v1|BU890153
1515
6511
492
96.4
globlastp

2

LAB633_H43
poplar|13v1|BU890153_P1
1516
6511
492
96.4
globlastp

2

LAB633_H43
primula|11v1|SRR098679X10
1517
6539
492
96.4
globlastp

3
0866_P1

LAB633_H43
primula|11v1|SRR098679X10
1518
6539
492
96.4
globlastp

4
0939_P1

LAB633_H43
pseudotsuga|10v1|SRR06511
1519
6507
492
96.4
globlastp

5
9S0015889

LAB633_H43
radish|gb164|EV545689
1520
6499
492
96.4
globlastp

6

LAB633_H43
radish|gb164|EW722850
1521
6499
492
96.4
globlastp

7

LAB633_H43
radish|gb164|EX754211
1522
6499
492
96.4
globlastp

8

LAB633_H43
rose|12v1|BQ105597
1523
6540
492
96.4
globlastp

9

LAB633_H44
rose|12v1|BQ105643
1524
6540
492
96.4
globlastp

0

LAB633_H44
salvia|10v1|CV165375
1525
6541
492
96.4
globlastp

1

LAB633_H44
soybean|11v1|GLYMA11G07
1526
6542
492
96.4
globlastp

2
980

LAB633_H44
soybean|12v1|GLYMA11G07
1527
6542
492
96.4
globlastp

2
980_P1

LAB633_H44
soybean|11v1|GLYMA13G03
1528
6502
492
96.4
globlastp

3
470

LAB633_H44
soybean|12v1|GLYMA13G03
1529
6502
492
96.4
globlastp

3
470_P1

LAB633_H44
soybean|11v1|GLYMA14G23
1530
6502
492
96.4
globlastp

4
990

LAB633_H44
soybean|12v1|GLYMA14G23
1531
6502
492
96.4
globlastp

4
990_P1

LAB633_H44
strawberry|11v1|CO379399
1532
6543
492
96.4
globlastp

5

LAB633_H44
switchgrass|12v1|DN143026_—
1533
6544
492
96.4
globlastp

6
P1

LAB633_H44
switchgrass|gb167|DN143026
1534
6544
492
96.4
globlastp

6

LAB633_H44
switchgrass|12v1|FE620863_—
1535
6544
492
96.4
globlastp

7
P1

LAB633_H44
switchgrass|gb167|FE620863
1536
6544
492
96.4
globlastp

7

LAB633_H44
taxus|10v1|SRR032523S0004
1537
6545
492
96.4
globlastp

8
485

LAB633_H44
thellungiella_halophilum|11v
1538
6499
492
96.4
globlastp

9
1|DN775718

LAB633_H45
thellungiella_parvulum|11v1|
1539
6499
492
96.4
globlastp

0
DN775718

LAB633_H45
thellungiella_parvulum|11v1|
1540
6499
492
96.4
globlastp

1
EPCRP019347

LAB633_H45
tobacco|gb162|CV017315
1541
6503
492
96.4
globlastp

2

LAB633_H45
tobacco|gb162|CV017984
1542
6503
492
96.4
globlastp

3

LAB633_H45
tripterygium|11v1|SRR09867
1543
6511
492
96.4
globlastp

4
7X104562

LAB633_H45
tripterygium|11v1|SRR09867
1544
6511
492
96.4
globlastp

5
7X106014

LAB633_H45
tripterygium|11v1|SRR09867
1545
6546
492
96.4
globlastp

6
7X111976

LAB633_H45
vinca|11v1|SRR098690X1133
1546
6547
492
96.4
globlastp

7
79

LAB633_H45
walnuts|gb166|CV196723
1547
6548
492
96.4
globlastp

8

LAB633_H45
zostera|10v1|AM408842
1548
6506
492
96.4
globlastp

9

LAB633_H78
nicotiana_benthamiana|12v1|
1549
6503
492
96.4
globlastp

4
CN746824_P1

LAB633_H46
fraxinus|11v1|SRR058827.10
1550
6549
492
96.36
glotblastn

0
4233_T1

LAB633_H46
phyla|11v2|SRR099037X138
1551
6550
492
96.36
glotblastn

1
679_T1

LAB633_H46
pteridium|11v1|SRR043594X
1552
6551
492
96.36
glotblastn

2
154147

LAB633_H46
salvia|10v1|FE537043
1553
6552
492
96.36
glotblastn

3

LAB633_H88
b_juncea|12v1|E6ANDIZ01A
1554
6553
492
95.8
globlastp

7
65VJ_P1

LAB633_H88
b_juncea|12v1|E6ANDIZ01A
1555
6553
492
95.8
globlastp

8
7IMJ_P1

LAB633_H88
b_juncea|12v1|E6ANDIZ01A
1556
6553
492
95.8
globlastp

9
HU2K_P1

LAB633_H89
b_juncea|12v1|E6ANDIZ01A
1557
6553
492
95.8
globlastp

0
JO5K_P1

LAB633_H89
b_juncea|12v1|E6ANDIZ01A
1558
6553
492
95.8
globlastp

1
T505_P1

LAB633_H89
b_juncea|12v1|E6ANDIZ01A
1559
6554
492
95.8
globlastp

2
XHQE_P1

LAB633_H89
b_juncea|12v1|E6ANDIZ01B
1560
6553
492
95.8
globlastp

3
2WAD_P1

LAB633_H89
b_juncea|12v1|E6ANDIZ01B
1561
6553
492
95.8
globlastp

4
8XA6_P1

LAB633_H89
b_juncea|12v1|E6ANDIZ01B
1562
6553
492
95.8
globlastp

5
BC2M_P1

LAB633_H89
b_juncea|12v1|E6ANDIZ01B
1563
6553
492
95.8
globlastp

6
BYKN_P1

LAB633_H89
b_juncea|12v1|E6ANDIZ01B
1564
6553
492
95.8
globlastp

7
JXUW2_P1

LAB633_H89
b_juncea|12v1|E6ANDIZ01C
1565
6553
492
95.8
globlastp

8
B13K_P1

LAB633_H89
banana|12v1|ES434916_P1
1566
6553
492
95.8
globlastp

9

LAB633_H90
banana|12v1|FL666640_P1
1567
6553
492
95.8
globlastp

0

LAB633_H90
banana|12v1|MAGEN201200
1568
6553
492
95.8
globlastp

1
5299_P1

LAB633_H90
banana|12v1|MAGEN201201
1569
6553
492
95.8
globlastp

2
3147_P1

LAB633_H90
bean|12v2|SRR001334.12319
1570
6555
492
95.8
globlastp

3
4_P1

LAB633_H90
lettuce|12v1|DW045455_P1
1571
6556
492
95.8
globlastp

4

LAB633_H90
onion|12v1|CF434881_P1
1572
6557
492
95.8
globlastp

5

LAB633_H90
onion|12v1|CF435456_P1
1573
6557
492
95.8
globlastp

6

LAB633_H90
onion|12v1|SRR073446X106
1574
6557
492
95.8
globlastp

7
316D1_P1

LAB633_H90
pepper|12v1|CO906858_P1
1575
6558
492
95.8
globlastp

8

LAB633_H90
prunus_mume|13v1|BF71720
1576
6559
492
95.8
globlastp

9
8_P1

LAB633_H91
prunus_mume|13v1|BU04334
1577
6560
492
95.8
globlastp

0
6_P1

LAB633_H91
prunus_mume|13v1|BU57255
1578
6560
492
95.8
globlastp

1
7_P1

LAB633_H91
prunus_mume|13v1|CA85398
1579
6559
492
95.8
globlastp

2
5_P1

LAB633_H91
soybean|12v1|BE020400_P1
1580
6555
492
95.8
globlastp

3

LAB633_H91
zostera|12v1|SRR057351X10
1581
6561
492
95.8
globlastp

4
0909D1_P1

LAB633_H46
acacia|10v1|FS589942_P1
1582
6562
492
95.8
globlastp

4

LAB633_H46
ambrosia|11v1|SRR346935.1
1583
6563
492
95.8
globlastp

5
13388_P1

LAB633_H46
ambrosia|11v1|SRR346935.2
1584
6563
492
95.8
globlastp

6
04909_P1

LAB633_H46
ambrosia|11v1|SRR346943.1
1585
6564
492
95.8
globlastp

7
08120_P1

LAB633_H46
apple|11v1|CK900604_P1
1586
6559
492
95.8
globlastp

8

LAB633_H46
apple|11v1|CN489095_P1
1587
6560
492
95.8
globlastp

9

LAB633_H47
apple|11v1|CN492567_P1
1588
6560
492
95.8
globlastp

0

LAB633_H47
apple|11v1|CN901400_P1
1589
6560
492
95.8
globlastp

1

LAB633_H47
arabidopsis_lyrata|09v1|JGIA
1590
6553
492
95.8
globlastp

2
L012090_P1

LAB633_H47
arabidopsis_lyrata|09v1|JGIA
1591
6553
492
95.8
globlastp

3
L023973_P1

LAB633_H47
arabidopsis_lyrata|09v1|JGIA
1592
6553
492
95.8
globlastp

4
L024512_P1

LAB633_H47
arabidopsis|10v1|ATlG75630
1593
6565
492
95.8
globlastp

5
_P1

LAB633_H47
arabidopsis|10v1|AT2G16510
1594
6553
492
95.8
globlastp

6
_P1

LAB633_H47
arabidopsis|10v1|AT4G34720
1595
6553
492
95.8
globlastp

7
_P1

LAB633_H47
arabidopsis|10v1|AT4G38920
1596
6553
492
95.8
globlastp

8
_P1

LAB633_H47
arnica|11v1|SRR099034X101
1597
6566
492
95.8
globlastp

9
44_P1

LAB633_H48
arnica|11v1|SRR099034X107
1598
6563
492
95.8
globlastp

0
784_P1

LAB633_H48
arnica|11v1|SRR099034X121
1599
6563
492
95.8
globlastp

1
503_P1

LAB633_H48
avocado|10v1|CK748427_P1
1600
6567
492
95.8
globlastp

2

LAB633_H48
avocado|10v1|CK768014_P1
1601
6568
492
95.8
globlastp

3

LAB633_H48
b_juncea|10v2|E6ANDIZ01A
1602
6553
492
95.8
globlastp

4
65VJ

LAB633_H48
b_juncea|10v2|E6ANDIZ01A
1603
6553
492
95.8
globlastp

5
7IMJ

LAB633_H48
b_juncea|10v2|E6ANDIZ01A
1604
6553
492
95.8
globlastp

6
HU2K

LAB633_H48
b_juncea|10v2|E6ANDIZ01A
1605
6553
492
95.8
globlastp

7
JYKA

LAB633_H48
b_juncea|10v2|E6ANDIZ01A
1606
6553
492
95.8
globlastp

8
L5YG

LAB633_H48
b_juncea|10v2|E6ANDIZ01B
1607
6553
492
95.8
globlastp

9
8XA6

LAB633_H49
b_juncea|10v2|E6ANDIZ01B
1608
6553
492
95.8
globlastp

0
BC2M

LAB633_H49
b_juncea|10v2|E6ANDIZ01D
1609
6553
492
95.8
globlastp

1
9LBK

LAB633_H49
b_oleracea|gbl61|AM059534
1610
6553
492
95.8
globlastp

2
_P1

LAB633_H49
b_oleracea|gb161|DY027262_—
1611
6553
492
95.8
globlastp

3
P1

LAB633_H49
b_oleracea|gb161|DY029496_—
1612
6553
492
95.8
globlastp

4
P1

LAB633_H49
b_rapa|11v1|AT000515_P1
1613
6553
492
95.8
globlastp

5

LAB633_H49
b_rapa|11v1|BG544216_P1
1614
6553
492
95.8
globlastp

6

LAB633_H49
b_rapa|11v1|BQ791842_P1
1615
6553
492
95.8
globlastp

7

LAB633_H49
b_rapa|11v1|CD812344_P1
1616
6569
492
95.8
globlastp

8

LAB633_H49
b_rapa|11v1|CD823270_P1
1617
6553
492
95.8
globlastp

9

LAB633_H50
b_rapa|11v1|H74958_P1
1618
6553
492
95.8
globlastp

0

LAB633_H50
banana|10v1|ES431631
1619
6553
492
95.8
globlastp

1

LAB633_H50
banana|10v1|FL666640
1620
6553
492
95.8
globlastp

2

LAB633_H50
canola|11v1|CN726029_P1
1621
6553
492
95.8
globlastp

4

LAB633_H50
canola|11v1|CN733181_P1
1622
6553
492
95.8
globlastp

5

LAB633_H50
canola|11v1|CN735102_P1
1623
6553
492
95.8
globlastp

6

LAB633_H50
canola|11v1|CN826256_P1
1624
6553
492
95.8
globlastp

7

LAB633_H50
canola|11v1|CN829751_P1
1625
6553
492
95.8
globlastp

8

LAB633_H50
canola|11v1|DQ068175_P1
1626
6553
492
95.8
globlastp

9

LAB633_H51
canola|11v1|EE454783_P1
1627
6553
492
95.8
globlastp

0

LAB633_H51
canola|11v1|EE456100_P1
1628
6553
492
95.8
globlastp

1

LAB633_H51
canola|11v1|EE476229_P1
1629
6553
492
95.8
globlastp

2

LAB633_H51
canola|11v1|EE483618_P1
1630
6553
492
95.8
globlastp

3

LAB633_H51
canola|11v1|EE545969_P1
1631
6553
492
95.8
globlastp

4

LAB633_H51
canola|11v1|EV024287_P1
1632
6553
492
95.8
globlastp

5

LAB633_H51
canola|11v1|GR443245_P1
1633
6553
492
95.8
globlastp

6

LAB633_H51
catharanthus|11v1|EG554231
1634
6570
492
95.8
globlastp

7
_P1

LAB633_H51
centaurea|gb166|EH746240_P
1635
6571
492
95.8
globlastp

8
1

LAB633_H51
centaurea|gb166|EH781668_P
1636
6571
492
95.8
globlastp

9
1

LAB633_H52
centaurea|gb166|EL933730_P
1637
6571
492
95.8
globlastp

0
1

LAB633_H52
cephalotaxus|11v1|SRR06439
1638
6572
492
95.8
globlastp

1
5X104314_P1

LAB633_H52
chickpea|11v1|FE669019
1639
6562
492
95.8
globlastp

2

LAB633_H52
chickpea|13v2|FE669019_P1
1640
6562
492
95.8
globlastp

2

LAB633_H52
cichorium|gb171|EH708074_—
1641
6573
492
95.8
globlastp

3
P1

LAB633_H52
cirsium|11v1|SRR346952.100
1642
6571
492
95.8
globlastp

4
4285_P1

LAB633_H52
cirsium|11v1|SRR346952.107
1643
6571
492
95.8
globlastp

5
5157_P1

LAB633_H52
cirsium|11v1|SRR346952.113
1644
6566
492
95.8
globlastp

6
8663_P1

LAB633_H52
cirsium|11v1|SRR346952.117
1645
6571
492
95.8
globlastp

7
634_P1

LAB633_H52
cowpea|12v1|AF022925_P1
1646
6574
492
95.8
globlastp

8

LAB633_H52
cowpea|gb166|AF022925
1647
6574
492
95.8
globlastp

8

LAB633_H52
cowpea|12v1|FG833662_P1
1648
6555
492
95.8
globlastp

9

LAB633_H52
cowpea|gb166|FG833662
1649
6555
492
95.8
globlastp

9

LAB633_H53
cyamopsis|10v1|EG986846_P
1650
6575
492
95.8
globlastp

0
1

LAB633_H53
cyamopsis|10v1|EG986904_P
1651
6575
492
95.8
globlastp

1
1

LAB633_H53
cynara|gb167|GE587704_P1
1652
6571
492
95.8
globlastp

2

LAB633_H53
dandelion|10v1|DR402372_P
1653
6556
492
95.8
globlastp

3
1

LAB633_H53
eggplant|10v1|FS004904_P1
1654
6576
492
95.8
globlastp

4

LAB633_H53
euphorbia|11v1|BP953990_P1
1655
6577
492
95.8
globlastp

5

LAB633_H53
euphorbia|11v1|BP956377_P1
1656
6578
492
95.8
globlastp

6

LAB633_H53
euphorbia|11v1|DV140898_P
1657
6579
492
95.8
globlastp

7
1

LAB633_H53
flaveria|11v1|SRR149229.100
1658
6563
492
95.8
globlastp

8
682_P1

LAB633_H53
flaveria|11v1|SRR149229.101
1659
6563
492
95.8
globlastp

9
144_P1

LAB633_H54
flaveria|11v1|SRR149229.138
1660
6563
492
95.8
globlastp

0
381_P1

LAB633_H54
flaveria|11v1|SRR149229.151
1661
6563
492
95.8
globlastp

1
35_P1

LAB633_H54
flaveria|11v1|SRR149232.100
1662
6563
492
95.8
globlastp

2
048_P1

LAB633_H54
flaveria|11v1|SRR149238.344
1663
6563
492
95.8
globlastp

3
732_P1

LAB633_H54
grape|11v1|GSVIVT0100885
1664
6553
492
95.8
globlastp

4
7001_P1

LAB633_H54
grape|11v1|GSVIVT0102416
1665
6553
492
95.8
globlastp

5
5001_P1

LAB633_H54
guizotia|10v1|GE553934XX1
1666
6563
492
95.8
globlastp

6
_P1

LAB633_H54
guizotia|10v1|GE555882_P1
1667
6580
492
95.8
globlastp

7

LAB633_H54
hevea|10v1|EC600598_P1
1668
6581
492
95.8
globlastp

8

LAB633_H54
ipomoea_batatas|10v1|EE875
1669
6582
492
95.8
globlastp

9
038_P1

LAB633_H55
ipomoea_nil|10v1|BJ553845_—
1670
6583
492
95.8
globlastp

0
P1

LAB633_H55
jatropha|09v1|GH295876_P1
1671
6584
492
95.8
globlastp

1

LAB633_H55
lettuce|10v1|DW045455
1672
6556
492
95.8
globlastp

2

LAB633_H55
liquorice|gbl71|FS239750_P1
1673
6562
492
95.8
globlastp

3

LAB633_H55
lotus|09v1|AI967344_P1
1674
6562
492
95.8
globlastp

4

LAB633_H55
lotus|09v1|LLAW719316_P1
1675
6562
492
95.8
globlastp

5

LAB633_H55
lotus|09v1|LLBI417561_P1
1676
6562
492
95.8
globlastp

6

LAB633_H55
nasturtium|11v1|GH163683_P
1677
6562
492
95.8
globlastp

7
1

LAB633_H55
nasturtium|11v1|GH170991_P
1678
6585
492
95.8
globlastp

8
1

LAB633_H55
nasturtium|11v1|SRR032558.
1679
6562
492
95.8
globlastp

9
101099_P1

LAB633_H56
nicotiana_benthamiana|12v1|
1680
6576
492
95.8
globlastp

0
CN741678_P1

LAB633_H56
nicotiana_benthamiana|gb162|
1681
6576
492
95.8
globlastp

0
CN741678

LAB633_H56
oak|10v1|CU657672_P1
1682
6586
492
95.8
globlastp

1

LAB633_H56
oil_palm|11v1|EL688767_P1
1683
6587
492
95.8
globlastp

2

LAB633_H56
parthenium|10v1|GW777804_—
1684
6580
492
95.8
globlastp

3
P1

LAB633_H56
peanut|10v1|CD037907_P1
1685
6562
492
95.8
globlastp

4

LAB633_H56
peanut|10v1|EE123399_P1
1686
6562
492
95.8
globlastp

5

LAB633_H56
petunia|gb171|CV295702_P1
1687
6588
492
95.8
globlastp

7

LAB633_H56
phalaenopsis|11v1|CB033183
1688
6557
492
95.8
globlastp

8
_P1

LAB633_H56
phalaenopsis|11v1|CB034659
1689
6557
492
95.8
globlastp

9
_P1

LAB633_H57
phalaenopsis|11v1|SRR12577
1690
6557
492
95.8
globlastp

0
1.101114_P1

LAB633_H57
phalaenopsis|11v1|SRR12577
1691
6589
492
95.8
globlastp

1
1.1033780_P1

LAB633_H57
pigeonpea|11v1|GR472597_P
1692
6555
492
95.8
globlastp

2
1

LAB633_H57
pigeonpea|11v1|SRR054580X
1693
6555
492
95.8
globlastp

3
100195_P1

LAB633_H57
pigeonpea|11v1|SRR054580X
1694
6575
492
95.8
globlastp

4
105049_P1

LAB633_H57
poplar|10v1|AI161891
1695
6553
492
95.8
globlastp

5

LAB633_H57
poplar|13v1|AI161453_P1
1696
6553
492
95.8
globlastp

5

LAB633_H57
poplar|10v1|AI162669
1697
6553
492
95.8
globlastp

6

LAB633_H57
poplar|13v1|AI162669_P1
1698
6553
492
95.8
globlastp

6

LAB633_H57
potato|10v1|AJ487387_P1
1699
6576
492
95.8
globlastp

7

LAB633_H57
primula|11v1|SRR098679X10
1700
6553
492
95.8
globlastp

8
5958_P1

LAB633_H57
prunus|10v1|BF717208
1701
6559
492
95.8
globlastp

9

LAB633_H58
prunus|10v1|BU043346
1702
6560
492
95.8
globlastp

0

LAB633_H58
prunus|10v1|BU572557
1703
6560
492
95.8
globlastp

1

LAB633_H58
prunus|10v1|CA853985
1704
6559
492
95.8
globlastp

2

LAB633_H58
radish|gb164|EV526213
1705
6553
492
95.8
globlastp

3

LAB633_H58
radish|gb164|EV527059
1706
6553
492
95.8
globlastp

4

LAB633_H58
radish|gb164|EV527473
1707
6553
492
95.8
globlastp

5

LAB633_H58
radish|gb164|EV535171
1708
6553
492
95.8
globlastp

6

LAB633_H58
radish|gb164|EV536495
1709
6553
492
95.8
globlastp

7

LAB633_H58
radish|gb164|EW722892
1710
6553
492
95.8
globlastp

8

LAB633_H58
radish|gb164|EW723024
1711
6553
492
95.8
globlastp

9

LAB633_H59
radish|gb164|EX746799
1712
6553
492
95.8
globlastp

0

LAB633_H59
radish|gb164|EX772034
1713
6553
492
95.8
globlastp

1

LAB633_H59
radish|gb164|EX777106
1714
6553
492
95.8
globlastp

2

LAB633_H59
radish|gb164|EX886866
1715
6553
492
95.8
globlastp

3

LAB633_H59
radish|gb164|EX896158
1716
6553
492
95.8
globlastp

4

LAB633_H59
radish|gb164|EX897706
1717
6553
492
95.8
globlastp

5

LAB633_H59
radish|gb164|FD966674
1718
6553
492
95.8
globlastp

6

LAB633_H59
rhizophora|10v1|SRR005793S
1719
6590
492
95.8
globlastp

7
0031467

LAB633_H59
silene|11v1|GH292760
1720
6591
492
95.8
globlastp

8

LAB633_H59
solanum_phureja|09v1|SPHB
1721
6576
492
95.8
globlastp

9
G123887

LAB633_H60
solanum_phureja|09v1|SPHB
1722
6576
492
95.8
globlastp

0
G124463

LAB633_H60
soybean|11v1|GLYMA01G37
1723
6592
492
95.8
globlastp

1
320

LAB633_H60
soybean|12v1|GLYMA01G37
1724
6592
492
95.8
globlastp

1
320_P1

LAB633_H60
soybean|11v1|GLYMA02G05
1725
6555
492
95.8
globlastp

2
630

LAB633_H60
soybean|12v1|GLYMA02G05
1726
6555
492
95.8
globlastp

2
630_P1

LAB633_H60
soybean|11v1|GLYMA04G00
1727
6555
492
95.8
globlastp

3
950

LAB633_H60
soybean|12v1|GLYMA04G00
1728
6555
492
95.8
globlastp

3
950_P1

LAB633_H60
soybean|11v1|GLYMA06G00
1729
6555
492
95.8
globlastp

4
980

LAB633_H60
soybean|12v1|GLYMA06G00
1730
6555
492
95.8
globlastp

4
980_P1

LAB633_H60
soybean|11v1|GLYMA11G11
1731
6575
492
95.8
globlastp

5
760

LAB633_H60
soybean|12v1|GLYMA11G11
1732
6575
492
95.8
globlastp

5
760_P1

LAB633_H60
soybean|11v1|GLYMA12G04
1733
6575
492
95.8
globlastp

6
090

LAB633_H60
soybean|12v1|GLYMA12G04
1734
6575
492
95.8
globlastp

6
090T2_P1

LAB633_H60
soybean|11v1|GLYMA16G24
1735
6555
492
95.8
globlastp

7
220

LAB633_H60
soybean|12v1|GLYMA16G24
1736
6555
492
95.8
globlastp

7
220_P1

LAB633_H60
spruce|11v1|ES252681
1737
6593
492
95.8
globlastp

8

LAB633_H60
spurge|gbl61|DV140898
1738
6579
492
95.8
globlastp

9

LAB633_H61
strawberry|11v1|AI795150
1739
6594
492
95.8
globlastp

0

LAB633_H61
strawberry|11v1|CO380387
1740
6594
492
95.8
globlastp

1

LAB633_H61
sunflower|12v1|CD848168
1741
6563
492
95.8
globlastp

2

LAB633_H61
sunflower|12v1|DY915982
1742
6563
492
95.8
globlastp

3

LAB633_H61
tea|10v1|GE650613
1743
6595
492
95.8
globlastp

4

LAB633_H61
thalictrum|11v1|SRR096787X
1744
6596
492
95.8
globlastp

5
10562

LAB633_H61
thellungiella_halophilum|11v
1745
6553
492
95.8
globlastp

6
1|BY804483

LAB633_H61
thellungiella_halophilum|11v
1746
6553
492
95.8
globlastp

7
1|DN778897

LAB633_H61
thellungiella_parvulum|11v1|
1747
6553
492
95.8
globlastp

8
EC599604

LAB633_H61
tobacco|gb162|CV016246
1748
6597
492
95.8
globlastp

9

LAB633_H62
tobacco|gb162|EB678620
1749
6576
492
95.8
globlastp

0

LAB633_H62
tobacco|gb162|X95751
1750
6598
492
95.8
globlastp

1

LAB633_H62
tobacco|gb162|X95752
1751
6576
492
95.8
globlastp

2

LAB633_H62
tomato|11v1|BG123887
1752
6576
492
95.8
globlastp

3

LAB633_H62
tomato|11v1|BG124463
1753
6576
492
95.8
globlastp

4

LAB633_H62
tragopogon|10v1|SRR020205
1754
6556
492
95.8
globlastp

5
S0002204

LAB633_H62
zostera|10v1|SRR057351S000
1755
6561
492
95.8
globlastp

6
3924

LAB633_H62
ambrosia|11v1|SRR346935.1
1756
6599
492
95.76
glotblastn

7
53166_T1

LAB633_H62
gossypium_raimondii|12v1|G
1757
6600
492
95.76
glotblastn

8
HU13670_T1

LAB633_H62
onion|gb162|CF435456
1758
6601
492
95.76
glotblastn

9

LAB633_H63
platanus|11v1|SRR096786X1
1759
6602
492
95.76
glotblastn

0
52052_T1

LAB633_H91
b_juncea|12v1|E6ANDIZ01A
1760
6603
492
95.2
globlastp

5
8FCE_P1

LAB633_H91
b_juncea|12v1|E6ANDIZ01B
1761
6604
492
95.2
globlastp

6
8WYM_P1

LAB633_H91
blueberry|12v1|SRR353282X
1762
6605
492
95.2
globlastp

7
19012D1_P1

LAB633_H91
lettuce|12v1|DW051555_P1
1763
6606
492
95.2
globlastp

8

LAB633_H91
pepper|12v1|BM060269_P1
1764
6607
492
95.2
globlastp

9

LAB633_H92
pepper|12v1|BM060287_P1
1765
6607
492
95.2
globlastp

0

LAB633_H92
pepper|12v1|BM062536_P1
1766
6608
492
95.2
globlastp

1

LAB633_H92
pepper|12v1|BM062580_P1
1767
6607
492
95.2
globlastp

2

LAB633_H63
acacia|10v1|FS585870_P1
1768
6609
492
95.2
globlastp

1

LAB633_H63
amborella|12v3|CK747014_P
1769
6610
492
95.2
globlastp

2
1

LAB633_H63
apple|11v1|CN489524_P1
1770
6611
492
95.2
globlastp

3

LAB633_H63
aristolochia|10v1|SRR039082
1771
6612
492
95.2
globlastp

4
S0037087_P1

LAB633_H63
arnica|11v1|SRR099034X113
1772
6613
492
95.2
globlastp

5
896_P1

LAB633_H63
arnica|11v1|SRR099034X139
1773
6606
492
95.2
globlastp

6
968_P1

LAB633_H63
b_rapa|11v1|CD817357_P1
1774
6614
492
95.2
globlastp

7

LAB633_H63
b_rapa|11v1|CD819176_P1
1775
6615
492
95.2
globlastp

8

LAB633_H63
canola|11v1|EV030960_P1
1776
6616
492
95.2
globlastp

9

LAB633_H64
canola|11v1|SRR329661.3704
1777
6617
492
95.2
globlastp

0
94_P1

LAB633_H64
castorbean|12v1|EE260752_P
1778
6618
492
95.2
globlastp

1
1

LAB633_H64
cedrus|11v1|SRR065007X100
1779
6619
492
95.2
globlastp

2
260_P1

LAB633_H64
centaurea|gb166|EH738599_P
1780
6620
492
95.2
globlastp

3
1

LAB633_H64
cephalotaxus|11v1|SRR06439
1781
6621
492
95.2
globlastp

4
5X104333_P1

LAB633_H64
chickpea|11v1|FE670073
1782
6609
492
95.2
globlastp

5

LAB633_H64
chickpea|13v2|FE670073_P1
1783
6609
492
95.2
globlastp

5

LAB633_H64
chickpea|11v1|SRR133517.11
1784
6609
492
95.2
globlastp

6
3981

LAB633_H64
chickpea|13v2|GR912192_P1
1785
6609
492
95.2
globlastp

6

LAB633_H64
cirsium|11v1|SRR346952.101
1786
6622
492
95.2
globlastp

7
5153_P1

LAB633_H64
cirsium|11v1|SRR346952.127
1787
6623
492
95.2
globlastp

8
159_P1

LAB633_H64
clover|gb162|BB928874_P1
1788
6609
492
95.2
globlastp

9

LAB633_H65
cryptomeria|gb166|BP175787
1789
6624
492
95.2
globlastp

0
_P1

LAB633_H65
cryptomeria|gb166|BY878554
1790
6625
492
95.2
globlastp

1
_P1

LAB633_H65
curcuma|10v1|DY391579_P1
1791
6626
492
95.2
globlastp

2

LAB633_H65
cynara|gb167|GE587668_P1
1792
6622
492
95.2
globlastp

3

LAB633_H65
dandelion|10v1|DR403014_P
1793
6627
492
95.2
globlastp

4
1

LAB633_H65
distylium|11v1|SRR065077X
1794
6628
492
95.2
globlastp

5
10448_P1

LAB633_H65
eggplant|10v1|FS003157_P1
1795
6607
492
95.2
globlastp

6

LAB633_H65
eggplant|10v1|FS008042_P1
1796
6629
492
95.2
globlastp

7

LAB633_H65
eggplant|10v1|FS024666_P1
1797
6607
492
95.2
globlastp

8

LAB633_H65
euphorbia|11v1|SRR098678X
1798
6630
492
95.2
globlastp

9
130658_P1

LAB633_H66
gerbera|09v1|AJ750022_P1
1799
6631
492
95.2
globlastp

0

LAB633_H66
gerbera|09v1|AJ750922_P1
1800
6632
492
95.2
globlastp

1

LAB633_H66
grape|11v1|GSVIVT0100983
1801
6633
492
95.2
globlastp

2
2001_P1

LAB633_H66
ipomoea_nil|10v1|BJ554296_—
1802
6607
492
95.2
globlastp

3
P1

LAB633_H66
ipomoea_nil|10v1|BJ554580_—
1803
6607
492
95.2
globlastp

4
P1

LAB633_H66
lettuce|10v1|DW051555
1804
6606
492
95.2
globlastp

5

LAB633_H66
liquorice|gbl71|FS258203_P1
1805
6609
492
95.2
globlastp

6

LAB633_H66
lotus|09v1|AV426568_P1
1806
6609
492
95.2
globlastp

7

LAB633_H66
marchantia|gb166|BJ843111_—
1807
6634
492
95.2
globlastp

8
P1

LAB633_H66
marchantia|gb166|C95837_P1
1808
6634
492
95.2
globlastp

9

LAB633_H67
maritime_pine|10v1|BX25531
1809
6635
492
95.2
globlastp

0
6_P1

LAB633_H67
medicago|12v1|AW689836_P
1810
6609
492
95.2
globlastp

1
1

LAB633_H67
medicago|12v1|BF636341_P1
1811
6609
492
95.2
globlastp

2

LAB633_H67
nasturtium|11v1|GH165984_P
1812
6636
492
95.2
globlastp

3
1

LAB633_H67
nicotiana_benthamiana|12v1|
1813
6637
492
95.2
globlastp

4
X95752_P1

LAB633_H67
nicotiana_benthamiana|gb162|
1814
6637
492
95.2
globlastp

4
CN743417

LAB633_H67
oak|10v1|FP024881_P1
1815
6638
492
95.2
globlastp

5

LAB633_H67
oak|10v1|FP035224_P1
1816
6638
492
95.2
globlastp

6

LAB633_H67
parthenium|10v1|GW777451_—
1817
6639
492
95.2
globlastp

7
P1

LAB633_H67
peanut|10v1|CD038517_P1
1818
6609
492
95.2
globlastp

8

LAB633_H67
pepper|gb171|BM060269
1819
6607
492
95.2
globlastp

9

LAB633_H68
pepper|gb171|BM060287
1820
6607
492
95.2
globlastp

0

LAB633_H68
pepper|gb171|BM062536
1821
6608
492
95.2
globlastp

1

LAB633_H68
pepper|gb171|BM062580
1822
6607
492
95.2
globlastp

2

LAB633_H68
petunia|gb171|CV294015_P1
1823
6640
492
95.2
globlastp

3

LAB633_H68
petunia|gb171|CV294463_P1
1824
6607
492
95.2
globlastp

4

LAB633_H68
petunia|gb171|CV296321_P1
1825
6607
492
95.2
globlastp

5

LAB633_H68
petunia|gb171|CV296920_P1
1826
6607
492
95.2
globlastp

6

LAB633_H68
potato|10v1|BF153914_P1
1827
6629
492
95.2
globlastp

7

LAB633_H68
primula|11v1|SRR098679X12
1828
6641
492
95.2
globlastp

8
6927_P1

LAB633_H68
safflower|gb162|EL404742
1829
6642
492
95.2
globlastp

9

LAB633_H69
sciadopitys|10v1|SRR065035
1830
6643
492
95.2
globlastp

0
S0000720

LAB633_H69
sciadopitys|10v1|SRR065035
1831
6644
492
95.2
globlastp

1
S0053046

LAB633_H69
senecio|gb170|DY659496
1832
6622
492
95.2
globlastp

2

LAB633_H69
sequoia|10v1|SRR065044S00
1833
6645
492
95.2
globlastp

3
00919

LAB633_H69
sequoia|10v1|SRR065044S00
1834
6646
492
95.2
globlastp

4
10934

LAB633_H69
silene|11v1|GH292064
1835
6647
492
95.2
globlastp

5

LAB633_H69
solanum_phureja|09v1|SPHA
1836
6629
492
95.2
globlastp

6
A824831

LAB633_H69
solanum_phureja|09v1|SPHA
1837
6629
492
95.2
globlastp

7
F010228

LAB633_H69
solanum_phureja|09v1|SPHB
1838
6629
492
95.2
globlastp

8
G124348

LAB633_H69
sunflower|12v1|AJ828980
1839
6648
492
95.2
globlastp

9

LAB633_H70
sunflower|12v1|CD849428
1840
6639
492
95.2
globlastp

0

LAB633_H70
sunflower|12v1|CD857457
1841
6648
492
95.2
globlastp

1

LAB633_H70
sunflower|12v1|CF082790
1842
6639
492
95.2
globlastp

2

LAB633_H70
sunflower|12v1|DY907561
1843
6648
492
95.2
globlastp

3

LAB633_H70
tobacco|gb162|EB426153
1844
6649
492
95.2
globlastp

4

LAB633_H70
tomato|11v1|AA824831
1845
6629
492
95.2
globlastp

5

LAB633_H70
tomato|11v1|X95751
1846
6629
492
95.2
globlastp

6

LAB633_H70
tragopogon|10v1|SRR020205
1847
6639
492
95.2
globlastp

7
S0001460

LAB633_H70
trigonella|11v1|SRR066194X
1848
6650
492
95.2
globlastp

8
103686

LAB633_H70
trigonella|11v1|SRR066194X
1849
6609
492
95.2
globlastp

9
105520

LAB633_H71
trigonella|11v1|SRR066194X
1850
6609
492
95.2
globlastp

0
118875

LAB633_H71
vinca|11v1|SRR098690X1060
1851
6651
492
95.2
globlastp

1
63

LAB633_H71
vinca|11v1|SRR098690X1345
1852
6651
492
95.2
globlastp

2
44

LAB633_H92
zostera|12v1|SRR287819X11
1853
6652
492
95.15
glotblastn

3
6135D1_T1

LAB633_H71
cirsium|11v1|SRR346952.101
1854
6653
492
95.15
glotblastn

3
2989_T1

LAB633_H71
gossypium_raimondii|12v1|S
1855
6654
492
95.15
glotblastn

4
RR032881.152023_T1

LAB633_H71
nasturtium|11v1|SRR032558.
1856
6655
492
95.15
glotblastn

5
351072_T1

LAB633_H71
phalaenopsis|11v1|CB033601
1857
6656
492
95.15
glotblastn

6
_T1

LAB633_H71
phyla|11v2|SRR099035X141
1858
6657
492
95.15
glotblastn

7
876_T1

LAB633_H71
primula|11v1|SRR098679X12
1859
6658
492
95.15
glotblastn

8
2176_T1

LAB633_H71
thalictrum|11v1|SRR096787X
1860
6659
492
95.15
glotblastn

9
11182

LAB633_H72
chestnut|gb170|SRR006295S0
1861
6660
492
94.6
globlastp

0
000372_P1

LAB633_H72
cirsium|11v1|SRR346952.130
1862
6661
492
94.6
globlastp

1
427_P1

LAB633_H72
euphorbia|11v1|SRR098678X
1863
6662
492
94.6
globlastp

2
105764_P1

LAB633_H72
gerbera|09v1|AJ752133_P1
1864
6663
492
94.6
globlastp

3

LAB633_H72
guizotia|10v1|GE553211_P1
1865
6664
492
94.6
globlastp

4

LAB633_H72
lovegrass|gb167|DN483212_P
1866
6665
492
94.6
globlastp

5
1

LAB633_H72
marchantia|gb166|BJ852612_—
1867
6666
492
94.6
globlastp

6
P1

LAB633_H72
oak|10v1|CU656108_P1
1868
6660
492
94.6
globlastp

7

LAB633_H72
oak|10v1|DB997616_P1
1869
6667
492
94.6
globlastp

8

LAB633_H72
silene|11v1|SRR096785X102
1870
6668
492
94.6
globlastp

9
971

LAB633_H92
banana|12v1|MUAC12v1PRD
1871
6669
492
94.55
glotblastn

4
004325_T1

LAB633_H73
arabidopsis_lyrata|09v1|JGIA
1872
6670
492
94.55
glotblastn

0
L007835_T1

LAB633_H73
fraxinus|11v1|SRR058827.10
1873
6671
492
94.55
glotblastn

1
1146_T1

LAB633_H73
antirrhinum|gb166|AJ806196
1874
6672
492
94.5
globlastp

2
_P1

LAB633_H73
cirsium|11v1|SRR346952.131
1875
6673
492
94.5
globlastp

3
960_P1

LAB633_H73
euonymus|11v1|SRR070038X
1876
6674
492
94.5
globlastp

4
275752_P1

LAB633_H73
fagopyrum|11v1|SRR063689
1877
6675
492
94.5
globlastp

5
X100219_P1

LAB633_H73
fagopyrum|11v1|SRR063689
1878
6676
492
94.5
globlastp

6
X103959_P1

LAB633_H73
fagopyrum|11v1|SRR063689
1879
6677
492
94.5
globlastp

7
X104259_P1

LAB633_H73
fagopyrum|11v1|SRR063689
1880
6675
492
94.5
globlastp

8
X107251_P1

LAB633_H73
fagopyrum|11v1|SRR063703
1881
6678
492
94.5
globlastp

9
X107404_P1

LAB633_H74
fagopyrum|11v1|SRR063703
1882
6675
492
94.5
globlastp

0
X111206_P1

LAB633_H74
medicago|12v1|AA660999_P
1883
6679
492
94.5
globlastp

1
1

LAB633_H74
nicotiana_benthamiana|gb162|
1884
6680
492
94.5
globlastp

2
CN743981

LAB633_H74
pine|10v2|BX255316_P1
1885
6681
492
94.5
globlastp

3

LAB633_H74
podocarpus|10v1|SRR065014
1886
6682
492
94.5
globlastp

4
S0007955_P1

LAB633_H74
rye|12v1|DRR001012.223082
1887
6683
492
94.5
globlastp

5

LAB633_H74
silene|11v1|GH292886
1888
6684
492
94.5
globlastp

6

LAB633_H74
tobacco|gb162|DV159858
1889
6685
492
94.5
globlastp

7

LAB633_H74
tobacco|gb162|DW003201
1890
6680
492
94.5
globlastp

8

LAB633_H74
vinca|11v1|SRR098690X1045
1891
6686
492
94.5
globlastp

9
49

LAB633_H75
zamia|gb166|DT589580
1892
6687
492
94.5
globlastp

0

LAB633_H92
blueberry|12v1|SRR353282X
1893
6688
492
94.1
globlastp

5
37911D1_P1

LAB633_H75
antirrhinum|gb166|AJ790387
1894
6689
492
94
globlastp

1
_P1

LAB633_H75
chestnut|gb170|SRR006295S0
1895
6690
492
94
globlastp

2
012127_P1

LAB633_H75
apple|11v1|MDPRD009363_—
1896
6691
492
93.98
glotblastn

3
T1

LAB633_H92
bean|12v2|CA901622_T1
1897
6692
492
93.94
glotblastn

6

LAB633_H75
fagopyrum|11v1|SRR063689
1898
6693
492
93.94
glotblastn

5
X1149_T1

LAB633_H75
trigonella|11v1|SRR066198X
1899
6694
492
93.94
glotblastn

6
1080363

LAB633_H74
nicotiana_benthamiana|12v1|
1900
6695
492
93.9
globlastp

2
CN743981_P1

LAB633_H75
acacia|10v1|GR482333_P1
1901
6696
492
93.9
globlastp

7

LAB633_H75
bruguiera|gb166|BP940407_P
1902
6697
492
93.9
globlastp

8
1

LAB633_H75
cedrus|11v1|SRR065007X100
1903
6698
492
93.9
globlastp

9
670_P1

LAB633_H76
spikemoss|gb165|FE472216
1904
6699
492
93.9
globlastp

0

LAB633_H76
spikemoss|gb165|FE492704
1905
6699
492
93.9
globlastp

1

LAB633_H76
walnuts|gb166|EL900477
1906
6700
492
93.9
globlastp

2

LAB633_H76
wheat|10v2|CA625845
1907
6701
492
93.9
globlastp

3

LAB633_H76
ipomoea_batatas|10v1|DV036
1908
6702
492
93.4
globlastp

4
086_P1

LAB633_H76
fescue|gb161|DT698181_P1
1909
6703
492
93.3
globlastp

5

LAB633_H76
spruce|11v1|ES250042
1910
6704
492
93.3
globlastp

6

LAB633_H76
phalaenopsis|11v1|CK856722
1911
6705
492
92.9
glotblastn

7
_T1

LAB633_H76
abies|11v2|SRR098676X1298
1912
6706
492
92.8
globlastp

8
84_P1

LAB633_H76
ceratodon|10v1|SRR074890S
1913
6707
492
92.8
globlastp

9
0015626_P1

LAB633_H77
physcomitrella|10v1|AW1453
1914
6708
492
92.8
globlastp

0
77_P1

LAB633_H77
physcomitrella|10v1|AW7390
1915
6708
492
92.8
globlastp

1
68_P1

LAB633_H77
physcomitrella|10v1|BQ8277
1916
6708
492
92.8
globlastp

2
17_P1

LAB633_H77
pteridium|11v1|SRR043594X
1917
6709
492
92.8
globlastp

3
116683

LAB633_H77
spikemoss|gb165|DN837789
1918
6710
492
92.8
globlastp

4

LAB633_H77
spikemoss|gb165|FE426809
1919
6710
492
92.8
globlastp

5

LAB633_H77
rye|12v1|DRR001015.210174
1920
6711
492
92.73
glotblastn

6

LAB633_H77
basilicum|10v1|DY328364_P
1921
6712
492
92.7
globlastp

7
1

LAB633_H77
hevea|10v1|EC609905_P1
1922
6713
492
92.7
globlastp

8

LAB633_H77
walnuts|gb166|CV196465
1923
6714
492
92.7
globlastp

9

LAB633_H78
ceratodon|10v1|SRR074890S
1924
6715
492
92.2
globlastp

0
0030085_P1

LAB633_H78
ceratodon|10v1|SRR074890S
1925
6715
492
92.2
globlastp

1
0054257_P1

LAB633_H78
ceratodon|10v1|SRR074890S
1926
6716
492
92.2
globlastp

2
0083877_P1

LAB633_H78
fern|gb171|DK954061_P1
1927
6717
492
92.2
globlastp

3

LAB633_H78
nicotiana_benthamiana|gb162|
1928
6718
492
92.12
glotblastn

4
CN746824

LAB633_H92
b_juncea|12v1|E6ANDIZ01A
1929
6719
492
92.1
globlastp

7
573W_P1

LAB633_H92
b_juncea|12v1|E6ANDIZ01C
1930
6720
492
92.1
globlastp

8
T2PY_P1

LAB633_H92
zostera|12v1|SRR057351X18
1931
6721
492
91.6
globlastp

9
9445D1_P1

LAB633_H78
physcomitrella|10v1|AW1456
1932
6722
492
91.6
globlastp

5
28_P1

LAB633_H78
physcomitrella|10v1|BJ16631
1933
6723
492
91.6
globlastp

6
0_P1

LAB633_H78
pteridium|11v1|GW574806
1934
6724
492
91.6
globlastp

7

LAB633_H78
flaveria|11v1|SRR149229.102
1935
6725
492
91.5
globlastp

8
831_P1

LAB633_H78
poppy|11v1|SRR096789.1511
1936
6726
492
91.5
globlastp

9
23_P1

LAB633_H79
zostera|10v1|SRR057351S004
1937
6727
492
90.91
glotblastn

0
0057

LAB633_H79
epimedium|11v1|SRR013502.
1938
6728
492
90.4
globlastp

1
2653_P1

LAB633_H93
zostera|12v1|SRR057351X12
1939
6729
492
90.3
glotblastn

0
8134D1_T1

LAB633_H79
utricularia|11v1|SRR094438.1
1940
6730
492
89.7
globlastp

2
06519

LAB633_H79
fern|gb171|DK945424_P1
1941
6731
492
89.2
globlastp

3

LAB633_H79
safflower|gb162|EL400731
1942
6732
492
89.2
globlastp

4

LAB633_H79
pteridium|11v1|SRR043594X
1943
6733
492
89.09
glotblastn

5
185320

LAB633_H79
tea|10v1|CV013952
1944
6734
492
88.8
globlastp

6

LAB633_H79
amaranthus|10v1|SRR039411
1945
6735
492
88.5
globlastp

7
S0010523_P1

LAB633_H79
b_juncea|10v2|E6ANDIZ01A
1946
6736
492
88.5
globlastp

8
7QBZ

LAB633_H79
mesostigma|gb166|DN254417
1947
6737
492
88.5
globlastp

9
_P1

LAB633_H80
mesostigma|gb166|EC727008
1948
6737
492
88.5
globlastp

0
_P1

LAB633_H80
rye|12v1|DRR001012.11979
1949
6738
492
88.5
globlastp

1

LAB633_H80
zinnia|gbl71|AU292884
1950
6739
492
88.3
globlastp

2

LAB633_H80
cirsium|11v1|SRR346952.101
1951
6740
492
88
globlastp

3
0164_P1

LAB633_H80
cirsium|11v1|SRR349641.123
1952
6740
492
88
globlastp

4
187_P1

LAB633_H80
b_juncea|10v2|E6ANDIZ01A
1953
6741
492
87.9
globlastp

5
7UOU

LAB633_H93
chickpea|13v2|GR420845_T1
1954
6742
492
87.88
glotblastn

1

LAB633_H80
cirsium|11v1|SRR349641.128
1955
6743
492
87.4
globlastp

6
132_P1

LAB633_H80
eschscholzia|11v1|SRR01411
1956
6744
492
87.3
globlastp

7
6.119052_P1

LAB633_H80
foxtail_millet|11v3|SICRP018
1957
6745
492
87.3
globlastp

8
324_P1

LAB633_H80
guizotia|10v1|GE569499_T1
1958
6746
492
86.23
glotblastn

9

LAB633_H81
cycas|gb166|CB092281_P1
1959
6747
492
86.1
globlastp

0

LAB633_H81
chlamydomonas|gb162|AW67
1960
6748
492
86.06
glotblastn

1
6113_T1

LAB633_H81
rice|11v1|CI252454
1961
6749
492
85.5
globlastp

2

LAB633_H81
taxus|10v1|SRR032523S0003
1962
6750
492
85.2
globlastp

3
980

LAB633_H93
switchgrass|12v1|FL726205_—
1963
6751
492
84.9
globlastp

2
P1

LAB633_H81
leymus|gb166|EG392695_P1
1964
6752
492
84.8
globlastp

4

LAB633_H81
basilicum|10v1|DY332297_P
1965
6753
492
84.4
globlastp

5
1

LAB633_H81
onion|gb162|CF434881
1966
6754
492
84.3
globlastp

6

LAB633_H93
switchgrass|12v1|SRR408048
1967
6755
492
84.2
globlastp

3
.161684_P1

LAB633_H81
chestnut|gb170|SRR006295S0
1968
6756
492
83.7
globlastp

7
007214_P1

LAB633_H81
poppy|11v1|SRR096789.1776
1969
6757
492
83.6
globlastp

8
80_P1

LAB633_H81
b_juncea|10v2|E6ANDIZ01A
1970
6758
492
83
globlastp

9
R9Y5

LAB633_H82
banana|10v1|ES434616
1971
6759
492
83
globlastp

0

LAB633_H82
curcuma|10v1|DY386269_P1
1972
6760
492
83
globlastp

1

LAB633_H82
tea|10v1|GT969344
1973
6761
492
82.8
globlastp

2

LAB633_H82
pteridium|11v1|SRR043594X
1974
6762
492
82.6
globlastp

3
14056

LAB633_H82
rye|12v1|DRR001016.1634
1975
6763
492
82.4
globlastp

4

LAB633_H82
artemisia|10v1|EY036129_P1
1976
6764
492
81.8
globlastp

5

LAB633_H82
euonymus|11v1|SRR070038X
1977
6764
492
81.8
globlastp

6
105022_P1

LAB633_H82
fagopyrum|11v1|SRR063689
1978
6765
492
81.8
globlastp

7
X101360_P1

LAB633_H82
fagopyrum|11v1|SRR063703
1979
6765
492
81.8
globlastp

8
X135285_P1

LAB633_H82
hornbeam|12v1|SRR364455.2
1980
6764
492
81.8
globlastp

9
18571_P1

LAB633_H83
sarracenia|11v1|SRR192669.1
1981
6764
492
81.8
globlastp

0
13809

LAB633_H83
tea|10v1|GE652210
1982
6764
492
81.8
globlastp

1

LAB633_H83
thalictrum|11v1|SRR096787X
1983
6764
492
81.8
globlastp

2
101012

LAB633_H83
thalictrum|11v1|SRR096787X
1984
6764
492
81.8
globlastp

3
159032

LAB633_H83
utricularia|11v1|SRR094438.1
1985
6766
492
81.8
globlastp

4
18396

LAB633_H83
volvox|12v1|FD805229_P1
1986
6767
492
81.8
globlastp

5

LAB633_H83
volvox|gb162|BE453521
1987
6767
492
81.8
globlastp

5

LAB633_H83
bruguiera|gb166|BP943146_P
1988
6768
492
81.2
globlastp

6
1

LAB633_H83
cannabis|12v1|JK498119_P1
1989
6769
492
81.2
globlastp

7

LAB633_H83
cannabis|12v1|SOLX0004620
1990
6770
492
81.2
globlastp

8
2_P1

LAB633_H83
cacao|10v1|CGD0025650_T1
1991
6771
492
81.07
glotblastn

9

LAB633_H84
canola|11v1|EE562026_T1
1992
6772
492
80.93
glotblastn

0

LAB633_H93
barley|12v1|BF621839_T1
1993
6773
492
80.61
glotblastn

4

LAB633_H84
ostreococcus|gb162|XM00141
1994
6774
492
80.61
glotblastn

1
8882_T1

LAB633_H84
rye|12v1|DRR001012.459948
1995
6775
492
80.6
globlastp

2

LAB633_H84
eucalyptus|11v2|SRR001658
1996
6776
492
80.12
glotblastn

3
X7371_T1

LAB633_H84
b_juncea|10v2|E6ANDIZ01A
1997
6777
492
80
globlastp

4
573W

LAB633_H84
pea|11v1|GH719836_P1
1998
6777
492
80
globlastp

5

LAB634_H1
pseudoroegneria|gb167|FF350
1999
6778
493
94.9
globlastp

988

LAB634_H2
wheat|10v2|BE425353
2000
6779
493
94.9
globlastp

LAB634_H5
wheat|12v3|BE425353_P1
2001
6780
493
94
globlastp

LAB634_H3
rye|12v1|BE637264
2002
6781
493
92.2
globlastp

LAB634_H4
leymus|gb166|EG376082_P1
2003
6782
493
90.3
globlastp

LAB635_H9
wheat|12v3|AL826606_P1
2004
6783
494
96.2
globlastp

LAB635_H1
wheat|10v2|BE406517
2005
6783
494
96.2
globlastp

LAB635_H2
rye|12v1|BE588100
2006
6784
494
91.3
globlastp

LAB635_H3
pseudoroegneria|gb167|FF362
2007
6785
494
88.2
globlastp

055

LAB635_H4
barley|10v2|BE193614
2008
6786
494
88
globlastp

LAB635_H4
barley|12v1|BE193614_P1
2009
6786
494
88
globlastp

LAB635_H5
wheat|10v2|BF291652
2010
6787
494
87
globlastp

LAB635_H5, L
wheat|12v3|BE406517_P1
2011
6788
494
85.9
globlastp

AB635_H6

LAB635_H6
wheat|10v2|AL829848
2012
6789
494
85.5
globlastp

LAB635_H7
wheat|10v2|CK198297
2013
6790
494
83.61
glotblastn

LAB635_H8
oat|11v1|GR365487_P1
2014
6791
494
80
globlastp

LAB636_H10
wheat|12v3|BE403352_P1
2015
6792
495
99.8
globlastp

LAB636_H1
rye|12v1|DRR001012.126194
2016
6793
495
98.9
globlastp

LAB636_H11
wheat|12v3|BE426366_P1
2017
6794
495
98.5
globlastp

LAB636_H12
wheat|12v3|CA705468_P1
2018
6795
495
97.8
globlastp

LAB636_H13
wheat|12v3|ERR125574X528
2019
6796
495
96.7
globlastp

675D1_P1

LAB636_H14
wheat|12v3|CA633935_T1
2020
6797
495
94.14
glotblastn

LAB636_H2
brachypodium|12v1|BRADI4
2021
6798
495
93.4
globlastp

G16830_P1

LAB636_H15
wheat|12v3|CJ623427_P1
2022
6799
495
92.9
globlastp

LAB636_H16
wheat|12v3|CJ603244_P1
2023
6800
495
92.3
globlastp

LAB636_H17
wheat|12v3|BE403104_P1
2024
6801
495
92.1
globlastp

LAB636_H3
rye|12v1|DRR001012.221386
2025
6802
495
92
globlastp

LAB636_H4
wheat|10v2|BQ243840
2026
6803
495
91.6
globlastp

LAB636_H18
wheat|12v3|CA708510_P1
2027
6804
495
91.2
globlastp

LAB636_H4
wheat|12v3|BQ243840_P1
2028
6805
495
91
globlastp

LAB636_H5
rice|11v1|AU058178
2029
6806
495
88.9
globlastp

LAB636_H6
switchgrass|12v1|FL772792_—
2030
6807
495
88.6
globlastp

P1

LAB636_H6
switchgrass|gb167|FL752075
2031
6808
495
88.1
globlastp

LAB636_H7
foxtail_millet|11v3|PHY7SI0
2032
6809
495
87.5
globlastp

26189M_P1

LAB636_H8
maize|10v1|AI612281_P1
2033
6810
495
87.2
globlastp

LAB636_H9
sorghum|12v1|SB05G020870
2034
6811
495
86.9
globlastp

LAB770_H7
wheat|12v3|CA728913_P1
2035
6812
496
96.3
globlastp

LAB770_H7
wheat|12v3|CA728913_P1
2035
6812
617
90.5
globlastp

LAB637_H1
rye|12v1|DRR001012.140904
2036
6813
496
94.9
globlastp

LAB637_H1
rye|12v1|DRR001012.140904
2036
6813
617
88.6
globlastp

LAB637_H2
brachypodium|12v1|BRADI3
2037
6814
496
93.1
globlastp

G04440_P1

LAB637_H2
brachypodium|12v1|BRADI3
2037
6814
617
90.5
globlastp

G04440_P1

LAB637_H3
switchgrass|12v1|FL690184_—
2038
6815
496
92
globlastp

P1

LAB637_H3
switchgrass|12v1|FL690184_—
2038
6815
617
95.4
globlastp

P1

LAB637_H3, L
switchgrass|gb167|FL690184
2039
6815
496
92
globlastp

AB770_H1

LAB637_H3, L
switchgrass|gb167|FL690184
2039
6815
617
95.4
globlastp

AB770_H1

LAB637_H4, L
switchgrass|gb167|DN143610
2040
6816
496
91.7
globlastp

AB770_H2

LAB637_H4, L
switchgrass|gb167|DN143610
2040
6816
617
94.8
globlastp

AB770_H2

LAB637_H5, L
foxtail_millet|11v3|PHY7SI0
2041
6817
496
91.1
globlastp

AB770_H3
17691M_P1

LAB637_H5, L
foxtail_millet|11v3|PHY7SI0
2041
6817
617
94.8
globlastp

AB770_H3
17691M_P1

LAB637_H7
rice|11v1|AU162360
2042
6818
496
90.5
globlastp

LAB637_H7
rice|11v1|AU162360
2042
6818
617
89.3
globlastp

LAB637_H6, L
maize|10v1|BQ279765_P1
2043
6819
496
90.5
globlastp

AB770_H4

LAB637_H6, L
maize|10v1|BQ279765_P1
2043
6819
617
95.1
globlastp

AB770_H4

LAB637_H8, L
maize|10v1|DT535423_P1
2044
6820
496
89.1
globlastp

AB770_H5

LAB637_H8, L
maize|10v1|DT535423_P1
2044
6820
617
93.7
globlastp

AB770_H5

LAB637_H9, L
sugarcane|10v1|CA072247
2045
6821
496
85.7
globlastp

AB770_H6

LAB637_H9, L
sugarcane|10v1|CA072247
2045
6821
617
91.9
globlastp

AB770_H6

LAB638_H1
rye|12v1|DRR001012.500005
2046
6822
497
94.3
globlastp

LAB638_H2
rye|12v1|DRR001012.106893
2047
6823
497
92.9
globlastp

LAB638_H3
rye|12v1|DRR001012.211062
2048
6823
497
92.9
globlastp

LAB638_H8
wheat|12v3|CA605197_P1
2049
6824
497
91.4
globlastp

LAB638_H4
wheat|10v2|BE401581
2050
6824
497
91.4
globlastp

LAB638_H5
wheat|10v2|BE406265
2051
6824
497
91.4
globlastp

LAB638_H5
wheat|12v3|BE401581_P1
2052
6824
497
91.4
globlastp

LAB638_H6
wheat|10v2|BQ840966
2053
6825
497
91.4
globlastp

LAB638_H7
pseudoroegneria|gb167|FF363
2054
6826
497
81.4
globlastp

756

LAB657_H1
sugarcane|10v1|CA065162
2055
6827
497
81.4
globlastp

LAB657_H1
sugarcane|10v1|CA065162
2055
6827
513
92.9
globlastp

LAB657_H2
millet|10v1|EVO454PM0289
2056
6828
497
80
globlastp

70_P1

LAB657_H2
millet|10v1|EVO454PM0289
2056
6828
513
97.1
globlastp

70_P1

LAB657_H3
sorghum|12v1|SB06G022040
2057
6829
497
80
globlastp

LAB657_H3
sorghum|12v1|SB06G022040
2057
6829
513
92.9
globlastp

LAB639_H4
rye|12v1|DRR001012.208915
2058
6830
498
83.7
globlastp

LAB640_H1
rye|12v1|BE636799
2059
6831
499
96.8
globlastp

LAB640_H2, L
wheat|12v3|BE488290_P1
2060
6832
499
96.8
globlastp

AB640_H7

LAB640_H2
wheat|10v2|CD933714
2061
6833
499
95.7
globlastp

LAB640_H3
rye|12v1|DRR001012.112482
2062
6834
499
94.6
globlastp

LAB640_H4
rye|12v1|DRR001012.369542
2063
6835
499
92.6
globlastp

LAB640_H5
wheat|10v2|CA678891
2064
6836
499
91.5
globlastp

LAB640_H6
fescue|gb161|DT685234_P1
2065
6837
499
89.4
globlastp

LAB640_H7
wheat|10v2|BE419563
2066
—
499
88.2
globlastp

LAB640_H8
wheat|10v2|BE488290
2067
6838
499
86
globlastp

LAB640_H9
oat|11v1|GR320771_P1
2068
6839
499
85.6
globlastp

LAB640_H10
rye|12v1|DRR001012.259187
2069
6840
499
85.1
globlastp

LAB640_H11
oat|11v1|GR329111_P1
2070
6841
499
84.5
globlastp

LAB640_H12
barley|10v2|AV835029
2071
6842
499
83.9
globlastp

LAB640_H12
barley|12v1|AV835029_P1
2072
6842
499
83.9
globlastp

LAB640_H13
oat|11v1|SRR020741.148089
2073
6843
499
80
globlastp

_P1

LAB641_H2
wheat|10v2|BE499264
2074
6844
500
96.4
globlastp

LAB641_H1
rye|12v1|DRR001012.116715
2075
6845
500
93.8
globlastp

LAB641_H3
brachypodium|12v1|BRADI3
2076
6846
500
92.1
globlastp

G27670_P1

LAB641_H4
rice|11v1|BI813394
2077
6847
500
89.4
globlastp

LAB641_H5
foxtail_millet|11v3|EC612743
2078
6848
500
87.4
globlastp

_P1

LAB641_H7
sorghum|12v1|SB01G020590
2079
6849
500
86.4
globlastp

LAB641_H8
maize|10v1|AI612279_P1
2080
6850
500
85.3
globlastp

LAB641_H10
foxtail_millet|11v3|PHY7SI0
2081
6851
500
84.4
globlastp

34851M_P1

LAB641_H17
maize|10v1|AI621452_P1
2082
6852
500
83.3
globlastp

LAB641_H15
sorghum|12v1|SB01G046880
2083
6853
500
83.3
globlastp

LAB641_H14
rice|11v1|BI808766
2084
6854
500
83.28
glotblastn

LAB641_H18
brachypodium|12v1|BRADI1
2085
6855
500
82.8
globlastp

G75010_P1

LAB641_H19
wheat|12v3|BE499264_P1
2086
6856
500
82.4
globlastp

LAB641_H20
rye|12v1|BE586817_P1
2087
6857
500
80.4
globlastp

LAB641_H21
wheat|12v3|BF478432_T1
2088
6858
500
80.24
glotblastn

LAB642_H2
wheat|12v3|CA651174_P1
2089
6859
501
95.6
globlastp

LAB642_H1
rye|12v1|DRR001017.102246
2090
6860
501
93.5
globlastp

9

LAB647
barley|12v1|BI958652_P1
2091
6861
504
88.3
globlastp

LAB649_H1
b_rapa|11v1|EE410383_P1
2092
6862
505
98.7
globlastp

LAB649_H7
b_juncea|12v1|E6ANDIZ01C
2093
6863
505
97.6
globlastp

585V_P1

LAB649_H2
radish|gb164|EX897837
2094
6864
505
95.8
globlastp

LAB649_H3
thellungiella_parvulum|11v1|
2095
6865
505
93.5
globlastp

DN773696

LAB649_H4
arabidopsis_lyrata|09v1|JGIA
2096
6866
505
92.7
globlastp

L005384_P1

LAB649_H5
arabidopsis|10v1|AT1G64710
2097
6867
505
92.7
globlastp

_P1

LAB649_H6
thellungiella_halophilum|11v
2098
6868
505
92.2
globlastp

1|DN773696

LAB650_H1
gossypium_raimondii|12v1|B
2099
6869
506
98.6
globlastp

E053207_P1

LAB650_H2
cotton|11v1|AI726238_P1
2100
6870
506
96.7
globlastp

LAB650_H3
gossypium_raimondii|12v1|AI
2101
6871
506
96.4
globlastp

726238_P1

LAB650_H4
cacao|10v1|CU474291_P1
2102
6872
506
90.2
globlastp

LAB650_H5
cacao|10v1|CGD0008734_P1
2103
6873
506
86
globlastp

LAB650_H6
beech|11v1|SRR006293.1794
2104
6874
506
84.65
glotblastn

_T1

LAB650_H7
clementine|11v1|BQ623853_—
2105
6875
506
84
globlastp

P1

LAB650_H8
oak|10v1|DN949864_P1
2106
6876
506
83.1
globlastp

LAB650_H9
cassava|09v1|DB922130_P1
2107
6877
506
83
globlastp

LAB650_H10
chestnut|gbl70|SRR006295S0
2108
6878
506
83
globlastp

004705_P1

LAB650_H11
beech|11v1|SRR006294.4421
2109
6879
506
82.97
glotblastn

_T1

LAB650_H12
pigeonpea|11v1|SRR054580X
2110
6880
506
82.5
globlastp

126577_P1

LAB650_H13
soybean|11v1|GLYMA20G26
2111
6881
506
82
globlastp

580

LAB650_H13
soybean|12v1|GLYMA20G26
2112
6881
506
82
globlastp

580_P1

LAB650_H14
cassava|09v1|JGICASSAVA1
2113
6882
506
81.8
globlastp

624VALIDM1_P1

LAB650_H15
oak|10v1|SRR039739S01183
2114
6883
506
81.6
globlastp

16_P1

LAB650_H17
poplar|13v1|BI129523_P1
2115
6884
506
81.6
globlastp

LAB650_H16
monkeyflower|10v1|GO99706
2116
6885
506
81.4
globlastp

3

LAB650_H16
monkeyflower|12v1|GR12831
2117
6885
506
81.4
globlastp

9_P1

LAB650_H17
poplar|10v1|BI129523
2118
6886
506
81.3
globlastp

LAB650_H18
soybean|11v1|GLYMA10G40
2119
6887
506
81.3
globlastp

730

LAB650_H18
soybean|12v1|GLYMA10G40
2120
6887
506
81.3
globlastp

730_P1

LAB650_H19
watermelon|11v1|AM721949
2121
6888
506
81.1
globlastp

LAB650_H20
peanut|10v1|GO331868_T1
2122
6889
506
81.06
glotblastn

LAB650_H21
grape|11v1|GSVIVT0103791
2123
6890
506
80.9
globlastp

5001_P1

LAB650_H22
medicago|12v1|AW685152_P
2124
6891
506
80.9
globlastp

1

LAB650_H23
poplar|10v1|CN517402
2125
6892
506
80.9
globlastp

LAB650_H24
prunus|10v1|BU042480
2126
6893
506
80.9
globlastp

LAB650_H25
trigonella|11v1|SRR066194X
2127
6894
506
80.9
globlastp

100544

LAB650_H26
castorbean|12v1|EE257257_T
2128
6895
506
80.82
glotblastn

1

LAB650_H27
chelidonium|11v1|SRR08475
2129
6896
506
80.7
globlastp

2X101076_P1

LAB650_H38
prunus_mume|13v1|BU04248
2130
6897
506
80.6
globlastp

0_P1

LAB650_H23
poplar|13v1|CN517402_P1
2131
6898
506
80.6
globlastp

LAB650_H28
eucalyptus|11v2|CT985402_P
2132
6899
506
80.6
globlastp

1

LAB650_H29
solanum_phureja|09v1|SPHB
2133
6900
506
80.5
globlastp

G129553

LAB650_H30
tomato|11v1|BG129553
2134
6901
506
80.5
globlastp

LAB650_H33
nicotiana_benthamiana|12v1|
2135
6902
506
80.5
globlastp

CK283693_P1

LAB650_H31
aristolochia|10v1|FD763402_—
2136
6903
506
80.4
globlastp

P1

LAB650_H32
chickpea|11v1|GR405235
2137
6904
506
80.3
globlastp

LAB650_H32
chickpea|13v2|GR405235_P1
2138
6904
506
80.3
globlastp

LAB650_H39
nicotiana_benthamiana|12v1|
2139
6905
506
80.2
globlastp

EB692551_P1

LAB650_H33
nicotiana_benthamiana|gb162|
2140
6906
506
80.2
globlastp

CK283693

LAB650_H40
bean|12v2|CA910823_P1
2141
6907
506
80.1
globlastp

LAB650_H41
sesame|12v1|SESI12V141410
2142
6908
506
80.1
glotblastn

5P1_T1

LAB650_H34
cannabis|12v1|EW701633_P1
2143
6909
506
80.1
globlastp

LAB650_H35
melon|10v1|AM721949_P1
2144
6910
506
80.1
globlastp

LAB650_H36
beet|12v1|BQ490190_P1
2145
6911
506
80
globlastp

LAB650_H37
tobacco|gb162|CV016030
2146
6912
506
80
globlastp

LAB651_H1
switchgrass|gb167|DN150484
2147
6913
507
89.8
globlastp

LAB651_H2
sorghum|12v1|SB03G012910
2148
6914
507
84.7
globlastp

LAB651_H3
millet|10v1|EVO454PM0133
2149
6915
507
82.85
glotblastn

66_T1

LAB652_H1
switchgrass|12v1|FL699953_—
2150
6916
508
96.7
globlastp

P1

LAB652_H1
switchgrass|gb167|FL699953
2151
6916
508
96.7
globlastp

LAB652_H18
switchgrass|12v1|FL726103_—
2152
6917
508
96.3
globlastp

P1

LAB652_H2
cenchrus|gb166|EB654383_P
2153
6918
508
96.3
globlastp

1

LAB652_H3
millet|10v1|EVO454PM0191
2154
6919
508
96.3
globlastp

78_P1

LAB652_H4
maize|10v1|AW147035_P1
2155
6920
508
95.8
globlastp

LAB652_H5
sorghum|12v1|SB01G008390
2156
6921
508
95.8
globlastp

LAB652_H6
sugarcane|10v1|AA525684
2157
6922
508
95.4
globlastp

LAB652_H7
maize|10v1|AI861318_P1
2158
6923
508
93
globlastp

LAB652_H8
oat|11v1|CK780289_P1
2159
6924
508
91.7
globlastp

LAB652_H9
brachypodium|12v1|BRADI1
2160
6925
508
91.2
globlastp

G08900_P1

LAB652_H10
rye|12v1|DRR001012.110234
2161
6926
508
90.7
globlastp

XX1

LAB652_H11
barley|10v2|BF625316
2162
6927
508
90.3
globlastp

LAB652_H12
rice|11v1|BI806501
2163
6928
508
90.3
globlastp

LAB652_H13
wheat|10v2|BE488914
2164
6929
508
90.3
globlastp

LAB652_H13,
wheat|12v3|BE423280XX1_P
2165
6929
508
90.3
globlastp

LAB652_H14
1

LAB652_H14
wheat|10v2|BE423280
2166
6930
508
89.8
globlastp

LAB652_H15
leymus|gb166|EG388927_P1
2167
6931
508
88.9
globlastp

LAB652_H16
pseudoroegneria|gb167|FF354
2168
6932
508
84.3
globlastp

039

LAB652_H17
lolium|10v1|ES699861_P1
2169
6933
508
83.3
globlastp

LAB653_H1
switchgrass|12v1|FE602252_—
2170
6934
509
92.7
globlastp

P1

LAB653_H1
switchgrass|gb167|FE602252
2171
6934
509
92.7
globlastp

LAB653_H2
millet|10v1|CD725229_P1
2172
6935
509
91.6
globlastp

LAB653_H3
cenchrus|gb166|EB655845_P
2173
6936
509
90.4
globlastp

1

LAB653_H4
sugarcane|10v1|BU103389
2174
6937
509
86
globlastp

LAB653_H5
sorghum|12v1|SB01G041550
2175
6938
509
85.1
globlastp

LAB653_H6
maize|10v1|AI979554_P1
2176
6939
509
83.4
globlastp

LAB653_H7
foxtail_millet|11v3|PHY7SI0
2177
6940
509
81.8
globlastp

36525M_P1

LAB653_H8
leymus|gb166|EG383933_P1
2178
6941
509
80.1
globlastp

LAB653_H9
brachypodium|12v1|BRADI1
2179
6942
509
80
globlastp

G68670_P1

LAB654_H4
switchgrass|12v1|FE617373_—
2180
6943
510
88.8
globlastp

P1

LAB654_H5
switchgrass|12v1|FL691713_—
2181
6944
510
84.4
globlastp

P1

LAB654_H1
maize|10v1|AI943869_P1
2182
6945
510
82.9
globlastp

LAB654_H2
brachypodium|12v1|BRADI5
2183
6946
510
80.7
globlastp

G15377_P1

LAB654_H3
rice|11v1|BQ906477
2184
6947
510
80
glotblastn

LAB655_H1
switchgrass|12v1|FE624783_—
2185
6948
511
90.6
globlastp

P1

LAB655_H1
switchgrass|gb167|FE624783
2186
6949
511
90.1
globlastp

LAB655_H2
sorghum|12v1|SB06G017040
2187
6950
511
89.6
globlastp

LAB655_H3
maize|10v1|AI600705_P1
2188
6951
511
86.4
globlastp

LAB655_H4
sugarcane|10v1|CA069664
2189
6952
511
84.6
globlastp

LAB655_H5
rice|11v1|AU056428
2190
6953
511
84.4
globlastp

LAB655_H6
rye|12v1|DRR001012.145027
2191
6954
511
83.3
globlastp

LAB655_H7
barley|10v2|AV833450
2192
6955
511
82.8
globlastp

LAB655_H10
wheat|12v3|CD877355_P1
2193
6956
511
82.3
globlastp

LAB655_H8
millet|10v1|EVO454PM0124
2194
6957
511
82.2
globlastp

44_P1

LAB655_H9
brachypodium|12v1|BRADI1
2195
6958
511
82
globlastp

G54570_P1

LAB655_H11
switchgrass|12v1|FL741655_—
2196
6959
511
80.1
globlastp

P1

LAB657_H4
maize|10v1|BE640184_P1
2197
6960
513
94.3
globlastp

LAB657_H5
switchgrass|gb167|FL933929
2198
6961
513
92.9
globlastp

LAB657_H6
switchgrass|12v1|FL842338_—
2199
6962
513
91.4
globlastp

P1

LAB657_H6
switchgrass|gb167|FL842338
2200
6962
513
91.4
globlastp

LAB657_H7
maize|10v1|BM075068_P1
2201
6963
513
85.7
globlastp

LAB657_H8
oat|11v1|GO588823_P1
2202
6964
513
81.4
globlastp

LAB657_H9
rice|11v1|BI305745
2203
6965
513
80.3
globlastp

LAB657_H10
lolium|10v1|SRR029314S000
2204
6966
513
80
globlastp

9965_P1

LAB659_H2
switchgrass|12v1|FL912449_—
2205
6967
514
87.3
globlastp

P1

LAB659_H1
switchgrass|gb167|DN148892
2206
6968
514
86.7
globlastp

LAB660_H10
switchgrass|12v1|FL721296_—
2207
6969
515
96.6
globlastp

P1

LAB660_H1
sorghum|12v1|SB04G037680
2208
6970
515
96.3
globlastp

LAB660_H11
switchgrass|12v1|FL694575_—
2209
6971
515
95.7
globlastp

P1

LAB660_H2
sugarcane|10v1|CA079386
2210
6972
515
95.7
globlastp

LAB660_H3
maize|10v1|AI734594_P1
2211
6973
515
94.2
globlastp

LAB660_H4
millet|10v1|EVO454PM0652
2212
6974
515
92.7
globlastp

19_P1

LAB660_H5
rice|11v1|BI813701
2213
6975
515
86.7
globlastp

LAB660_H6
brachypodium|12v1|BRADI3
2214
6976
515
85.4
globlastp

G55860_P1

LAB660_H12
wheat|12v3|BF202425_T1
2215
6977
515
81.38
glotblastn

LAB660_H7
leymus|gb166|EG397532_P1
2216
6978
515
80.2
globlastp

LAB660_H8
rye|12v1|DRR001012.107103
2217
6979
515
80.1
globlastp

LAB660_H9
wheat|10v2|BE415043
2218
6980
515
80
glotblastn

LAB661_H1
millet|10v1|EVO454PM0713
2219
6981
516
96.3
globlastp

37_P1

LAB661_H8
switchgrass|12v1|FL701955_—
2220
6982
516
91.3
globlastp

P1

LAB661_H2
sorghum|12v1|SB09G023080
2221
6983
516
87.8
globlastp

LAB661_H3
maize|10v1|AW067087_P1
2222
6984
516
87.2
globlastp

LAB661_H4
sugarcane|10v1|CA066631
2223
6985
516
86.25
glotblastn

LAB661_H5
rice|11v1|AU085819
2224
6986
516
84
globlastp

LAB661_H9
switchgrass|12v1|GD008314_—
2225
6987
516
83.3
globlastp

P1

LAB661_H6
rice|11v1|AU085820
2226
6988
516
82.8
glotblastn

LAB661_H7
brachypodium|12v1|BRADI2
2227
6989
516
81.7
globlastp

G22630_P1

LAB662_H1
sorghum|12v1|AW678665
2228
6990
517
90.7
globlastp

LAB662_H2
switchgrass|12v1|FE597736_—
2229
6991
517
89.2
globlastp

P1

LAB662_H2
switchgrass|gb167|FE597736
2230
6991
517
89.2
globlastp

LAB662_H3
sugarcane|10v1|CA071116
2231
6992
517
88.4
globlastp

LAB662_H4
maize|10v1|AI600387_P1
2232
6993
517
86.8
globlastp

LAB662_H5
rice|11v1|AA754481
2233
6994
517
85.1
globlastp

LAB662_H6
brachypodium|12v1|BRADI4
2234
6995
517
83.5
globlastp

G39197_P1

LAB662_H7
rice|11v1|BE230153
2235
6996
517
80.3
globlastp

LAB663_H7
switchgrass|12v1|SRR187766
2236
6997
518
92.2
globlastp

.701178_P1

LAB663_H8
switchgrass|12v1|GD040220_—
2237
6998
518
90.5
globlastp

P1

LAB663_H1
sorghum|12v1|SB02G035910
2238
6999
518
89.1
globlastp

LAB663_H2
maize|10v1|AI783052_P1
2239
7000
518
85.7
globlastp

LAB663_H3
pseudoroegneria|gb167|FF346
2240
7001
518
81
globlastp

150

LAB663_H5
wheat|12v3|BQ842544_P1
2241
7002
518
81
globlastp

LAB663_H4
rye|12v1|DRR001012.138219
2242
7003
518
80.6
globlastp

LAB663_H5
wheat|10v2|BQ842544
2243
7004
518
80.6
globlastp

LAB663_H6
brachypodium|12v1|BRADI4
2244
7005
518
80.3
globlastp

G08120_P1

LAB664_H1
millet|10v1|EVO454PM0005
2245
7006
519
99.3
globlastp

26_P1

LAB664_H23
switchgrass|12v1|FE601121_—
2246
7007
519
97.9
globlastp

P1

LAB664_H2
maize|10v1|AI372152_P1
2247
7008
519
95
globlastp

LAB664_H3
sorghum|12v1|SB09G024810
2248
7009
519
95
globlastp

LAB664_H4
sugarcane|10v1|CA073888
2249
7009
519
95
globlastp

LAB664_H5
maize|10v1|BE511858_P1
2250
7010
519
92.9
globlastp

LAB664_H6
rice|11v1|AA750057
2251
7011
519
91.8
globlastp

LAB664_H7
leymus|gb166|EG391638_P1
2252
7012
519
90.4
globlastp

LAB664_H8
fescue|gb161|DT702544_P1
2253
7013
519
90
globlastp

LAB664_H9
barley|10v2|BE413105
2254
7014
519
89.3
globlastp

LAB664_H10
brachypodium|12v1|BRADI2
2255
7015
519
89
globlastp

G20570_P1

LAB664_H11
oat|11v1|CN814662_P1
2256
7016
519
89
globlastp

LAB664_H12
rye|12v1|DRR001012.145532
2257
7017
519
88.6
globlastp

LAB664_H13
wheat|10v2|BE404120
2258
7018
519
88.6
globlastp

LAB664_H13
wheat|12v3|BE493085_P1
2259
7018
519
88.6
globlastp

LAB664_H14
pseudoroegneria|gb167|FF350
2260
7019
519
88.57
glotblastn

314

LAB664_H15
rye|12v1|DRR001012.103031
2261
7020
519
87.86
glotblastn

LAB664_H16
lovegrass|gb167|EH184319_T
2262
7021
519
85.71
glotblastn

1

LAB664_H17
foxtail_millet|11v3|GT091141
2263
7022
519
84
globlastp

_P1

LAB664_H18
millet|10v1|CD724411_P1
2264
7023
519
84
globlastp

LAB664_H19
rice|11v1|AF225922
2265
7024
519
83.3
globlastp

LAB664_H24
switchgrass|12v1|FL896166_—
2266
7025
519
82.9
globlastp

P1

LAB664_H20
maize|10v1|AI978031_P1
2267
7026
519
82.9
globlastp

LAB664_H25
switchgrass|12v1|GD018645_—
2268
7027
519
82.6
globlastp

P1

LAB664_H21
cenchrus|gb166|EB654951_P
2269
7028
519
82.5
globlastp

1

LAB664_H22
wheat|10v2|CA497859
2270
7029
519
80.1
globlastp

LAB665_H1
cenchrus|gb166|EB652424_P
2271
520
520
100
globlastp

1

LAB665_H2
lovegrass|gb167|EH188259_P
2272
520
520
100
globlastp

1

LAB665_H3
maize|10v1|AA979992_P1
2273
520
520
100
globlastp

LAB665_H4
millet|10v1|EVO454PM0432
2274
520
520
100
globlastp

74_P1

LAB665_H5
rice|11v1|CB212121
2275
520
520
100
globlastp

LAB665_H6
sugarcane|10v1|CA082741
2276
520
520
100
globlastp

LAB665_H7
switchgrass|12v1|FL730481_—
2277
520
520
100
globlastp

P1

LAB665_H7
switchgrass|gb167|FL730481
2278
520
520
100
globlastp

LAB665_H8
switchgrass|12v1|FL730485_—
2279
520
520
100
globlastp

P1

LAB665_H8
switchgrass|gb167|FL730485
2280
7030
520
100
glotblastn

LAB665_H9
sorghum|12v1|SB06G033360
2281
7031
520
99.5
globlastp

LAB665_H10
maize|10v1|AI586586_P1
2282
7032
520
99
globlastp

LAB665_H11
brachypodium|12v1|BRADI5
2283
7033
520
98
globlastp

G26806_P1

LAB665_H12
oat|11v1|GO598723_P1
2284
7034
520
98
globlastp

LAB665_H13
pseudoroegneria|gb167|FF345
2285
7034
520
98
globlastp

898

LAB665_H20
wheat|12v3|BQ238405_P1
2286
7035
520
97.5
globlastp

1

LAB665_H14
rye|12v1|DRR001012.112075
2287
7035
520
97.5
globlastp

LAB665_H15
rye|12v1|DRR001012.154017
2288
7035
520
97.5
globlastp

LAB665_H16
wheat|10v2|BE352625
2289
7035
520
97.5
globlastp

LAB665_H17
barley|10v2|BE420600XX1
2290
7036
520
97
globlastp

LAB665_H18
wheat|12v3|BE402395_P1
2291
7037
520
97
globlastp

LAB665_H18
wheat|10v2|BF202015
2292
7038
520
96.6
globlastp

LAB665_H17
barley|12v1|BE420600_P1
2293
7039
520
96.1
globlastp

LAB665_H19
rye|12v1|DRR001012.108092
2294
7040
520
96.1
globlastp

LAB665_H20
fescue|gb161|DT683669_P1
2295
7041
520
93.6
globlastp

LAB665_H20
banana|12v1|BBS3243T3_P1
2296
7042
520
93.1
globlastp

2

LAB665_H20
banana|12v1|FF559212_P1
2297
7043
520
93.1
globlastp

3

LAB665_H21
banana|10v1|BBS3243T3
2298
7044
520
92.6
globlastp

LAB665_H22
banana|10v1|FF559212
2299
7045
520
92.6
globlastp

LAB665_H23
phalaenopsis|11v1|SRR12577
2300
7046
520
92.08
glotblastn

1.1036098_T1

LAB665_H24
oil_palm|11v1|EL682034_P1
2301
7047
520
91.6
globlastp

LAB665_H25
oil_palm|11v1|EY396282_P1
2302
7048
520
91.6
globlastp

LAB665_H26
ambrosia|11v1|SRR346935.3
2303
7049
520
90.6
globlastp

1340_P1

LAB665_H27
euonymus|11v1|SRR070038X
2304
7050
520
90.6
globlastp

125124_P1

LAB665_H28
flaveria|11v1|SRR149229.128
2305
7051
520
90.6
globlastp

76_P1

LAB665_H29
flaveria|11v1|SRR149232.176
2306
7051
520
90.6
globlastp

966_P1

LAB665_H30
oil_palm|11v1|EL683871_P1
2307
7052
520
90.6
globlastp

LAB665_H31
phalaenopsis|11v1|SRR12577
2308
7053
520
90.6
globlastp

1.105004_P1

LAB665_H32
sunflower|12v1|DY914938
2309
7049
520
90.6
globlastp

LAB665_H33
sunflower|12v1|DY921222
2310
7049
520
90.6
globlastp

LAB665_H20
pepper|12v1|CO775983_P1
2311
7054
520
90.1
globlastp

4

LAB665_H20
zostera|12v1|AM767783_P1
2312
7055
520
90.1
globlastp

5

LAB665_H34
amorphophallus|11v2|SRR08
2313
7056
520
90.1
globlastp

9351X119460_P1

LAB665_H35
apple|11v1|CO066264_P1
2314
7057
520
90.1
globlastp

LAB665_H36
cucumber|09v1|DV632421_P
2315
7058
520
90.1
globlastp

1

LAB665_H37
curcuma|10v1|DY388180_T1
2316
7059
520
90.1
glotblastn

LAB665_H38
euonymus|11v1|SRR070038X
2317
7060
520
90.1
globlastp

220957_P1

LAB665_H39
grape|11v1|GSVIVT0102685
2318
7061
520
90.1
globlastp

1001_P1

LAB665_H40
melon|10v1|DV632421_P1
2319
7058
520
90.1
globlastp

LAB665_H41
nasturtium|11v1|SRR032558.
2320
7062
520
90.1
globlastp

104418_P1

LAB665_H42
pepper|gb171|CO775983
2321
7054
520
90.1
globlastp

LAB665_H43
solanum_phureja|09v1|SPHB
2322
7063
520
90.1
globlastp

G134742

LAB665_H44
watermelon|11v1|DV632421
2323
7058
520
90.1
globlastp

LAB665_H45
zostera|10v1|AM767783
2324
7055
520
90.1
globlastp

LAB665_H20
nicotiana_benthamiana|12v1|
2325
7064
520
89.6
globlastp

6
EB425041_P1

LAB665_H20
nicotiana_benthamiana|12v1|
2326
7065
520
89.6
globlastp

7
EB425373_P1

LAB665_H20
olea|13v1|SRR014464X2967
2327
7066
520
89.6
globlastp

8
D1_P1

LAB665_H20
prunus_mume|13v1|CB82128
2328
7067
520
89.6
globlastp

9
6_P1

LAB665_H46
ambrosia|11v1|SRR346935.1
2329
7068
520
89.6
globlastp

4014_P1

LAB665_H47
amsonia|11v1|SRR098688X1
2330
7069
520
89.6
globlastp

01442_P1

LAB665_H48
apple|11v1|CN877976_P1
2331
7070
520
89.6
globlastp

LAB665_H49
aristolochia|10v1|SRR039082
2332
7071
520
89.6
globlastp

S0195342_P1

LAB665_H50
blueberry|10v1|DR067023
2333
7072
520
89.6
globlastp

LAB665_H50
blueberry|12v1|DR067023_P1
2334
7072
520
89.6
globlastp

LAB665_H51
cannabis|12v1|JK498315_P1
2335
7073
520
89.6
globlastp

LAB665_H52
castorbean|12v1|EG660628_P
2336
7074
520
89.6
globlastp

1

LAB665_H53
catharanthus|11v1|SRR09869
2337
7069
520
89.6
globlastp

1X13789_P1

LAB665_H54
clementine|11v1|CV884222_—
2338
7075
520
89.6
globlastp

P1

LAB665_H55
orange|11v1|CV884222_P1
2338
7075
520
89.6
globlastp

LAB665_H56
cotton|11v1|BG441601_P1
2339
7076
520
89.6
globlastp

LAB665_H57
cucurbita|11v1|SRR091276X
2340
7077
520
89.6
globlastp

101458_P1

LAB665_H58
eschscholzia|11v1|CD480050
2341
7078
520
89.6
globlastp

_P1

LAB665_H59
gossypium_raimondii|12v1|B
2342
7076
520
89.6
globlastp

G441601_P1

LAB665_H60
phyla|11v2|SRR099037X224
2343
7079
520
89.6
globlastp

103_P1

LAB665_H61
prunus|10v1|CB821286
2344
7067
520
89.6
globlastp

LAB665_H62
rose|12v1|EC587004
2345
7080
520
89.6
globlastp

LAB665_H63
tabernaemontana|11v1|SRR09
2346
7069
520
89.6
globlastp

8689X105357

LAB665_H64
tobacco|gb162|EB425041
2347
7065
520
89.6
globlastp

LAB665_H65
tripterygium|11v1|SRR09867
2348
7081
520
89.6
globlastp

7X118644

LAB665_H66
abies|11v2|SRR098676X1375
2349
7082
520
89.1
globlastp

8_P1

LAB665_H67
cacao|10v1|CU472895_P1
2350
7083
520
89.1
globlastp

LAB665_H68
cichorium|gb171|EH673922_—
2351
7084
520
89.1
globlastp

P1

LAB665_H69
cichorium|gb171|EL362644_—
2352
7085
520
89.1
globlastp

P1

LAB665_H70
cirsium|11v1|SRR346952.100
2353
7084
520
89.1
globlastp

8524_P1

LAB665_H71
cirsium|11v1|SRR346952.105
2354
7084
520
89.1
globlastp

703_P1

LAB665_H72
cirsium|11v1|SRR346952.111
2355
7084
520
89.1
globlastp

6817_P1

LAB665_H73
coffea|10v1|DV663807_P1
2356
7086
520
89.1
globlastp

LAB665_H74
cotton|11v1|BQ405654_P1
2357
7087
520
89.1
globlastp

LAB665_H75
euonymus|11v1|SRR070038X
2358
7088
520
89.1
globlastp

106975_P1

LAB665_H76
euphorbia|11v1|BI962048_P1
2359
7089
520
89.1
globlastp

LAB665_H77
gossypium_raimondii|12v1|B
2360
7087
520
89.1
globlastp

Q405654_P1

LAB665_H78
maritime_pine|10v1|CT57851
2361
7090
520
89.1
globlastp

0_P1

LAB665_H79
oak|10v1|DB998869_P1
2362
7091
520
89.1
globlastp

LAB665_H80
pine|10v2|AI812693_P1
2363
7090
520
89.1
globlastp

LAB665_H81
platanus|11v1|SRR096786X1
2364
7092
520
89.1
globlastp

05416_P1

LAB665_H82
pseudotsuga|10v1|SRR06511
2365
7082
520
89.1
globlastp

9S0016350

LAB665_H83
safflower|gb162|EL410612
2366
7084
520
89.1
globlastp

LAB665_H84
scabiosa|11v1|SRR063723X1
2367
7093
520
89.1
globlastp

10095

LAB665_H85
strawberry|11v1|DV439471
2368
7094
520
89.1
globlastp

LAB665_H86
tobacco|gb162|EB425373
2369
7095
520
89.1
globlastp

LAB665_H87
tomato|11v1|BG134742
2370
7096
520
89.1
globlastp

LAB665_H88
tragopogon|10v1|SRR020205
2371
7097
520
89.1
globlastp

S0044553

LAB665_H89
tripterygium|11v1|SRR09867
2372
7098
520
89.1
globlastp

7X10565

LAB665_H92
olea|13v1|SRR014463X37018
2373
7099
520
89.1
globlastp

D1_P1

LAB665_H90
onion|gb162|CF441137
2374
7100
520
88.73
glotblastn

LAB665_H91
fraxinus|11v1|SRR058827.11
2375
7101
520
88.61
glotblastn

4618_T1

LAB665_H92
olea|11v1|SRR014463.37018
2376
7102
520
88.61
glotblastn

LAB665_H21
lettuce|12v1|DW048831_P1
2377
7103
520
88.6
globlastp

0

LAB665_H93
beet|12v1|BQ060495_P1
2378
7104
520
88.6
globlastp

LAB665_H94
cassava|09v1|CK650539_P1
2379
7105
520
88.6
globlastp

LAB665_H95
centaurea|gb166|EH718958_P
2380
7106
520
88.6
globlastp

1

LAB665_H96
centaurea|gb166|EH726695_P
2381
7107
520
88.6
globlastp

1

LAB665_H97
cephalotaxus|11v1|SRR06439
2382
7108
520
88.6
globlastp

5X104104_P1

LAB665_H98
cirsium|11v1|SRR346952.100
2383
7106
520
88.6
globlastp

3274_P1

LAB665_H99
cirsium|11v1|SRR346952.100
2384
7109
520
88.6
globlastp

4063_P1

LAB665_H10
cirsium|11v1|SRR346952.103
2385
7110
520
88.6
globlastp

0
982_P1

LAB665_H10
cirsium|11v1|SRR346952.688
2386
7106
520
88.6
globlastp

1
630_P1

LAB665_H10
cowpea|12v1|FF384587_P1
2387
7111
520
88.6
globlastp

2

LAB665_H10
cowpea|gb166|FF384587
2388
7111
520
88.6
globlastp

2

LAB665_H10
cynara|gb167|GE589630_P1
2389
7112
520
88.6
globlastp

3

LAB665_H10
distylium|11v1|SRR065077X
2390
7108
520
88.6
globlastp

4
108220_P1

LAB665_H10
eucalyptus|11v2|CU395885_P
2391
7113
520
88.6
globlastp

5
1

LAB665_H10
euonymus|11v1|SRR070038X
2392
7114
520
88.6
globlastp

6
245901_P1

LAB665_H10
guizotia|10v1|GE562490_P1
2393
7115
520
88.6
globlastp

7

LAB665_H10
lettuce|10v1|DW048831
2394
7103
520
88.6
globlastp

8

LAB665_H10
lotus|09v1|LLAW163924_P1
2395
7111
520
88.6
globlastp

9

LAB665_H11
podocarpus|10v1|SRR065014
2396
7108
520
88.6
globlastp

0
S0005514_P1

LAB665_H11
poplar|10v1|AI162253
2397
7116
520
88.6
globlastp

1

LAB665_H11
rhizophora|10v1|SRR005793S
2398
7117
520
88.6
globlastp

2
0001754

LAB665_H11
spruce|11v1|ES864294
2399
7118
520
88.6
globlastp

3

LAB665_H21
onion|12v1|CF441137_P1
2400
7119
520
88.2
globlastp

1

LAB665_H11
poplar|13v1|AI162253_P1
2401
7120
520
88.2
globlastp

1

LAB665_H11
poppy|11v1|SRR030259.1255
2402
7121
520
88.12
glotblastn

4
78_T1

LAB665_H21
bean|12v2|CA911163_P1
2403
7122
520
88.1
globlastp

2

LAB665_H11
amborella|12v2|SRR038634.2
2404
7123
520
88.1
globlastp

5
6901

LAB665_H11
bean|12v1|CA911163
2405
7122
520
88.1
globlastp

6

LAB665_H11
chestnut|gb170|SRR006295S0
2406
7124
520
88.1
globlastp

7
022473_P1

LAB665_H11
dandelion|10v1|DY825393_P
2407
7125
520
88.1
globlastp

8
1

LAB665_H11
monkeyflower|10v1|GO95903
2408
7126
520
88.1
globlastp

9
2

LAB665_H11
monkeyflower|12v1|GR06867
2409
7126
520
88.1
globlastp

9
4_P1

LAB665_H12
oak|10v1|CU656269_P1
2410
7124
520
88.1
globlastp

0

LAB665_H12
papaya|gb165|EX256097_P1
2411
7127
520
88.1
globlastp

1

LAB665_H12
petunia|gb171|CV299439_P1
2412
7128
520
88.1
globlastp

2

LAB665_H12
phyla|11v2|SRR099035X139
2413
7129
520
88.1
globlastp

3
714_P1

LAB665_H12
sciadopitys|10v1|SRR065035
2414
7130
520
88.1
globlastp

4
S0012181

LAB665_H12
soybean|11v1|GLYMA10G38
2415
7131
520
88.1
globlastp

5
120

LAB665_H12
soybean|12v1|GLYMA10G38
2416
7131
520
88.1
globlastp

5
125_P1

LAB665_H12
soybean|11v1|GLYMA20G29
2417
7132
520
88.1
globlastp

6
700

LAB665_H12
soybean|12v1|GLYMA20G29
2418
7132
520
88.1
globlastp

6
700_P1

LAB665_H12
taxus|10v1|SRR032523S0010
2419
7133
520
88.1
globlastp

7
724

LAB665_H12
triphysaria|10v1|EY144131
2420
7134
520
88.1
globlastp

8

LAB665_H12
artemisia|10v1|EY037160_T1
2421
7135
520
87.62
glotblastn

9

LAB665_H13
poppy|11v1|SRR096789.1045
2422
7121
520
87.62
glotblastn

0
91_T1

LAB665_H21
aquilegia|10v2|DT730739_P1
2423
7136
520
87.6
globlastp

3

LAB665_H13
aquilegia|10v1|DT730739
2424
7137
520
87.6
globlastp

1

LAB665_H13
eucalyptus|11v2|CD669026_P
2425
7138
520
87.6
globlastp

2
1

LAB665_H13
fagopyrum|11v1|SRR063689
2426
7139
520
87.6
globlastp

3
X219139_P1

LAB665_H13
ipomoea_nil|10v1|BJ558772_—
2427
7140
520
87.6
globlastp

4
P1

LAB665_H13
medicago|12v1|AW981006_P
2428
7141
520
87.6
globlastp

5
1

LAB665_H13
pigeonpea|11v1|SRR054580X
2429
7142
520
87.6
globlastp

6
110091_P1

LAB665_H13
thalictrum|11v1|SRR096787X
2430
7143
520
87.6
globlastp

7
10242

LAB665_H13
trigonella|11v1|SRR066194X
2431
7141
520
87.6
globlastp

8
244665

LAB665_H13
tripterygium|11v1|SRR09867
2432
7144
520
87.6
globlastp

9
7X101877

LAB665_H21
onion|12v1|SRR073446X174
2433
7145
520
87.3
globlastp

4
49D1_P1

LAB665_H14
beech|11v1|SRR006293.1165
2434
7146
520
87.13
glotblastn

0
1_T1

LAB665_H14
vinca|11v1|SRR098690X2071
2435
7147
520
87.13
glotblastn

1
27

LAB665_H14
chickpea|11v1|FE669148
2436
7148
520
87.1
globlastp

2

LAB665_H14
chickpea|13v2|FE669148_P1
2437
7148
520
87.1
globlastp

2

LAB665_H14
cleome_spinosa|10v1|GR9314
2438
7149
520
87.1
globlastp

3
06_P1

LAB665_H14
fagopyrum|11v1|SRR063689
2439
7150
520
87.1
globlastp

4
X10482XX1_P1

LAB665_H14
flax|11v1|GW866721_P1
2440
7151
520
87.1
globlastp

5

LAB665_H14
grape|11v1|GSVIVT0102898
2441
7152
520
87.1
globlastp

6
5001_P1

LAB665_H14
safflower|gb162|EL399119
2442
7153
520
87.1
globlastp

7

LAB665_H14
beech|11v1|SRR006293.2146
2443
7154
520
86.63
glotblastn

8
4_T1

LAB665_H14
sarracenia|11v1|SRR192669.1
2444
7155
520
86.63
glotblastn

9
27721

LAB665_H15
eggplant|10v1|FS026985_P1
2445
7156
520
86.6
globlastp

0

LAB665_H15
potato|10v1|BG096434_P1
2446
7157
520
86.6
globlastp

1

LAB665_H15
potato|10v1|CK862208_P1
2447
7158
520
86.6
globlastp

2

LAB665_H15
solanum_phureja|09v1|SPHB
2448
7157
520
86.6
globlastp

3
G134632

LAB665_H15
tobacco|gb162|EB436339
2449
7159
520
86.6
globlastp

4

LAB665_H15
peanut|10v1|ES722255_P1
2450
7160
520
86.3
globlastp

5

LAB665_H15
utricularia|11v1|SRR094438.1
2451
7161
520
86.14
glotblastn

6
03057

LAB665_H15
nasturtium|11v1|SRR032558.
2452
7162
520
86.1
globlastp

7
125288_P1

LAB665_H15
platanus|11v1|SRR096786X1
2453
7163
520
86.1
globlastp

8
3023_P1

LAB665_H15
radish|gb164|EW731504
2454
7164
520
86.1
globlastp

9

LAB665_H16
solanum_phureja|09v1|SPHAI
2455
7165
520
86.1
globlastp

0
483593

LAB665_H16
thellungiella_halophilum|11v
2456
7166
520
86.1
globlastp

1
1|BY827571

LAB665_H16
thellungiella_parvulum|11v1|
2457
7166
520
86.1
globlastp

2
BY827571

LAB665_H21
pepper|12v1|CA516783_P1
2458
7167
520
85.6
globlastp

5

LAB665_H16
canola|11v1|DY011019_P1
2459
7168
520
85.6
globlastp

3

LAB665_H16
pepper|gb171|CA516783
2460
7167
520
85.6
globlastp

4

LAB665_H16
primula|11v1|SRR098679X10
2461
7169
520
85.6
globlastp

5
1965_P1

LAB665_H16
radish|gb164|EV524582
2462
7170
520
85.6
globlastp

6

LAB665_H16
silene|11v1|GH292861
2463
7171
520
85.6
globlastp

7

LAB665_H16
silene|11v1|SRR096785X140
2464
7172
520
85.6
globlastp

8
356

LAB665_H16
triphysaria|10v1|BM357298
2465
7173
520
85.6
globlastp

9

LAB665_H17
hornbeam|12v1|SRR364455.1
2466
7174
520
85.15
glotblastn

0
08780_T1

LAB665_H21
b_juncea|12v1|E6ANDIZ01B
2467
7175
520
85.1
globlastp

6
0B7B_P1

LAB665_H21
b_juncea|12v1|E6ANDIZ01B
2468
7176
520
85.1
globlastp

7
MX69_P1

LAB665_H17
arabidopsis_lyrata|09v1|JGIA
2469
7177
520
85.1
globlastp

1
L025551_P1

LAB665_H17
b_oleracea|gb161|DY027041_—
2470
7176
520
85.1
globlastp

2
P1

LAB665_H17
b_rapa|11v1|CD814800_P1
2471
7176
520
85.1
globlastp

3

LAB665_H17
b_rapa|11v1|CD817890_P1
2472
7178
520
85.1
globlastp

4

LAB665_H17
canola|11v1|EG019998_P1
2473
7176
520
85.1
globlastp

5

LAB665_H17
radish|gb164|EX756827
2474
7179
520
85.1
globlastp

6

LAB665_H17
tomato|11v1|BG134632
2475
7180
520
85.1
globlastp

7

LAB665_H17
arabidopsis|10v1|AT4G25550
2476
7181
520
84.7
globlastp

8
_P1

LAB665_H17
b_juncea|10v2|E6ANDIZ01D
2477
7182
520
84.7
globlastp

9
72XG

LAB665_H18
gnetum|10v1|DN954711_P1
2478
7183
520
84.7
globlastp

0

LAB665_H18
potato|10v1|BG594190_P1
2479
7184
520
84.7
globlastp

1

LAB665_H18
radish|gb164|EV529337
2480
7185
520
84.7
globlastp

2

LAB665_H21
b_juncea|12v1|E6ANDIZ01C
2481
7186
520
84.2
globlastp

8
D18A_P1

LAB665_H18
b_rapa|11v1|CD822007_P1
2482
7186
520
84.2
globlastp

3

LAB665_H18
canola|11v1|EE452927_P1
2483
7186
520
84.2
globlastp

4

LAB665_H18
cynara|gb167|GE598725_P1
2484
7187
520
84.2
globlastp

5

LAB665_H18
radish|gb164|EV536302
2485
7188
520
84.2
globlastp

6

LAB665_H18
cycas|gb166|EX924706_P1
2486
7189
520
83.7
globlastp

7

LAB665_H18
ambrosia|11v1|SRR346943.1
2487
7190
520
83.66
glotblastn

8
10084_T1

LAB665_H18
wheat|10v2|DR737429
2488
7191
520
83.25
glotblastn

9

LAB665_H19
clover|gb162|BB916672_P1
2489
7192
520
83.2
globlastp

0

LAB665_H19
arnica|11v1|SRR099034X123
2490
7193
520
83.17
glotblastn

1
140_T1

LAB665_H19
vinca|11v1|SRR098690X1068
2491
7194
520
83.17
glotblastn

2
49

LAB665_H19
centaurea|gb166|EH717510_T
2492
7195
520
82.67
glotblastn

3
1

LAB665_H19
ceratodon|10v1|AW087005_P
2493
7196
520
82.2
globlastp

4
1

LAB665_H19
marchantia|gb166|BJ844093_—
2494
7197
520
82.2
globlastp

5
P1

LAB665_H19
physcomitrella|10v1|BJ19829
2495
7196
520
82.2
globlastp

6
5_P1

LAB665_H19
eschscholzia|11v1|SRR01411
2496
7198
520
81.2
globlastp

7
6.109721_P1

LAB665_H21
olea|13v1|SRR014464X40446
2497
7199
520
80.7
globlastp

9
D1_P1

LAB665_H19
spikemoss|gb165|FE452878
2498
7200
520
80.7
globlastp

8

LAB665_H19
ambrosial|11v1|SRR346947.1
2499
7201
520
80.3
glotblastn

9
63642_T1

LAB665_H20
cedrus|11v1|SRR065007X109
2500
7202
520
80.2
globlastp

0
893_P1

LAB666_H1
millet|10v1|CD725078_P1
2501
7203
521
94.3
globlastp

LAB666_H2
switchgrass|12v1|DN144222_—
2502
7204
521
94.3
globlastp

P1

LAB666_H2
switchgrass|gb167|DN144222
2503
7204
521
94.3
globlastp

LAB666_H3
switchgrass|12v1|FE633933_—
2504
7205
521
94.3
globlastp

P1

LAB666_H3
switchgrass|gb167|FE633933
2505
7205
521
94.3
globlastp

LAB666_H4
sorghum|12v1|SB09G021980
2506
7206
521
93.1
globlastp

LAB666_H5
maize|10v1|AI372214_P1
2507
7207
521
92.6
globlastp

LAB666_H6
cynodon|10v1|ES296768_P1
2508
7208
521
90.3
globlastp

LAB666_H7
sugarcane|10v1|CA080464
2509
7209
521
90.3
globlastp

LAB666_H8
sorghum|12v1|SB04G004800
2510
7210
521
88.6
globlastp

LAB666_H9
maize|10v1|BG837406_T1
2511
7211
521
86.86
glotblastn

LAB667_H1
switchgrass|12v1|DN143859_—
2512
7212
522
93.6
globlastp

P1

LAB667_H1
switchgrass|gb167|DN143859
2513
7213
522
93.6
globlastp

LAB667_H12
switchgrass|12v1|FE604583_—
2514
7214
522
92.8
globlastp

P1

LAB667_H2
sorghum|12v1|SB02G029480
2515
7215
522
91.5
globlastp

LAB667_H3
sugarcane|10v1|CA069945
2516
7216
522
90.6
globlastp

LAB667_H4
maize|10v1|BE511154_P1
2517
7217
522
87.8
globlastp

LAB667_H5
maize|10v1|BG837077_P1
2518
7218
522
87.7
globlastp

LAB667_H6
rice|11v1|BI813601
2519
7219
522
81.9
globlastp

LAB667_H7
oat|11v1|GR343968_T1
2520
7220
522
81.82
glotblastn

LAB667_H10
wheat|12v3|CA646540_P1
2521
7221
522
81.7
globlastp

LAB667_H8
millet|10v1|EVO454PM0335
2522
7222
522
81.3
globlastp

71_P1

LAB667_H9
barley|10v2|BQ761834
2523
7223
522
81.2
globlastp

LAB667_H10
wheat|10v2|AL826210
2524
7224
522
81.19
glotblastn

LAB667_H11
brachypodium|12v1|BRADI4
2525
7225
522
80.4
globlastp

G34820_P1

LAB668_H1
millet|10v1|EVO454PM0105
2526
7226
523
97.7
globlastp

18_P1

LAB668_H2
cenchrus|gb166|EB661950_P
2527
7227
523
97
globlastp

1

LAB668_H3
switchgrass|12v1|FE614179_—
2528
7228
523
96.6
globlastp

P1

LAB668_H3
switchgrass|gb167|DN145311
2529
7229
523
96.2
globlastp

LAB668_H4
sugarcane|10v1|CA072523
2530
7230
523
95.9
globlastp

LAB668_H5
maize|10v1|AI979603_P1
2531
7231
523
94.7
globlastp

LAB668_H6
sorghum|12v1|SB02G040620
2532
7232
523
93.7
globlastp

LAB668_H7
cynodon|10v1|ES299677_P1
2533
7233
523
92.9
globlastp

LAB668_H8
maize|10v1|AI622591_P1
2534
7234
523
92.9
globlastp

LAB668_H9
rice|11v1|AU101972
2535
7235
523
91
globlastp

LAB668_H10
pseudoroegneria|gb167|FF345
2536
7236
523
89.3
globlastp

069

LAB668_H11
barley|10v2|BE421662
2537
7237
523
88.5
globlastp

LAB668_H12
fescue|gb161|DT685490_P1
2538
7238
523
88.5
globlastp

LAB668_H13
oat|11v1|GR352405_P1
2539
7239
523
88.1
globlastp

LAB668_H14
rye|12v1|DRR001012.103256
2540
7240
523
88.1
globlastp

LAB668_H24
wheat|12v3|BE403245_P1
2541
7241
523
87.8
globlastp

LAB668_H15
leymus|gb166|EG398694_P1
2542
7242
523
87.8
globlastp

LAB668_H16
rye|12v1|DRR001012.113351
2543
7243
523
87.8
globlastp

LAB668_H17
wheat|10v2|BE400825
2544
7244
523
87.8
globlastp

LAB668_H17
wheat|12v3|BE400825_P1
2545
7245
523
87.4
globlastp

LAB668_H18
brachypodium|12v1|BRADI1
2546
7246
523
85.8
globlastp

G20080_P1

LAB668_H19
rye|12v1|DRR001012.132819
2547
7247
523
82.5
globlastp

LAB668_H20
foxtail_millet|11v3|PHY7SI0
2548
7248
523
80.8
globlastp

36853M_P1

LAB668_H21
millet|10v1|EVO454PM0074
2549
7249
523
80.6
globlastp

82_P1

LAB668_H22
pseudoroegneria|gb167|FF360
2550
7250
523
80.2
globlastp

520

LAB668_H25
switchgrass|12v1|FL793498_—
2551
7251
523
80.1
globlastp

P1

LAB668_H23
switchgrass|gb167|DN141485
2552
7252
523
80.1
globlastp

LAB669_H1
switchgrass|12v1|FL750676_—
2553
7253
524
98.1
globlastp

P1

LAB669_H89
switchgrass|12v1|E597846_—
2554
7254
524
97.9
globlastp

P1

LAB669_H1
switchgrass|gb167|FE597846
2555
7255
524
97.8
globlastp

LAB669_H2
sorghum|12v1|SB01G037170
2556
7256
524
97.65
glotblastn

LAB669_H3
maize|10v1|AW120158_P1
2557
7257
524
97
globlastp

LAB669_H4
maize|10v1|AI396559_P1
2558
7258
524
96.8
globlastp

LAB669_H5
rye|12v1|DRR001012.115999
2559
7259
524
96
globlastp

LAB669_H6
wheat|10v2|BQ166811
2560
7259
524
96
globlastp

LAB669_H6
wheat|12v3|BQ246981_P1
2561
7260
524
95.7
globlastp

LAB669_H8
brachypodium|12v1|BRADI1
2562
7261
524
95.3
globlastp

G64190_P1

LAB669_H9
fescue|gb161|CK801052_P1
2563
7262
524
94.9
globlastp

LAB669_H90
wheat|12v3|BE516939_P1
2564
7263
524
92.1
globlastp

LAB669_H91
banana|12v1|ES436911_P1
2565
7264
524
89.4
globlastp

LAB669_H10
clementine|11v1|CX071916_—
2566
7265
524
88.8
globlastp

P1

LAB669_H11
orange|11v1|CX071916_P1
2567
7265
524
88.8
globlastp

LAB669_H12
soybean|11v1|GLYMA19G40
2568
7266
524
88.5
globlastp

600

LAB669_H12
soybean|12v1|GLYMA19G40
2569
7266
524
88.5
globlastp

600_P1

LAB669_H13
pigeonpea|11v1|SRR054580X
2570
7267
524
88.2
globlastp

100999_P1

LAB669_H14
soybean|11v1|GLYMA02G01
2571
7268
524
88.2
globlastp

390

LAB669_H14
soybean|12v1|GLYMA02G01
2572
7268
524
88.2
globlastp

390_P1

LAB669_H15
pigeonpea|11v1|SRR054580X
2573
7269
524
88.1
globlastp

100166_P1

LAB669_H16
soybean|11v1|GLYMA03G37
2574
7270
524
88.1
globlastp

980

LAB669_H16
soybean|12v2|GLYMA03G37
2575
7270
524
88.1
globlastp

980_P1

LAB669_H17
zostera|10v1|SRR057351S000
2576
7271
524
87.97
glotblastn

6025

LAB669_H18
medicago|12v1|AA660189_P
2577
7272
524
87.8
globlastp

1

LAB669_H19
eucalyptus|11v2|ES590896_P
2578
7273
524
87.7
globlastp

1

LAB669_H20
amborella|12v3|FD432109_P
2579
7274
524
87.6
globlastp

1

LAB669_H92
bean|12v2|FG229598_P1
2580
7275
524
87.5
globlastp

LAB669_H93
sesame|12v1|SESI12V138279
2581
7276
524
87.5
globlastp

5_P1

LAB669_H21
bean|12v1|FG229598
2582
7275
524
87.5
globlastp

LAB669_H22
cotton|11v1|CO111529XX1_—
2583
7277
524
87.5
globlastp

P1

LAB669_H23
arabidopsis_lyrata|09v1|CRP
2584
7278
524
87.4
globlastp

ALE021972_P1

LAB669_H24
gossypium_raimondii|12v1|B
2585
7279
524
87.4
globlastp

Q405139_P1

LAB669_H25
grape|11v1|GSVIVT0102815
2586
7280
524
87.4
globlastp

3001_P1

LAB669_H26
trigonella|11v1|SRR066194X
2587
7281
524
87.4
glotblastn

147217

LAB669_H27
cacao|10v1|CU469910_P1
2588
7282
524
87.3
globlastp

LAB669_H28
cucumber|09v1|AM727579_P
2589
7283
524
87.3
globlastp

1

LAB669_H29
castorbean|12v1|EG663818_P
2590
7284
524
87.2
globlastp

1

LAB669_H30
watermelon|11v1|AM727579
2591
7285
524
87.2
globlastp

LAB669_H31
poppy|11v1|SRR030259.1029
2592
7286
524
87.1
globlastp

63_P1

LAB669_H94
chickpea|13v2|FE670490_P1
2593
7287
524
87
globlastp

LAB669_H32
cannabis|12v1|JK494800_P1
2594
7288
524
87
globlastp

LAB669_H33
thellungiella_parvulum|11v1|
2595
7289
524
87
globlastp

BY816163

LAB669_H95
lettuce|12v1|DW070893_P1
2596
7290
524
86.9
globlastp

LAB669_H96
prunus_mume|13v1|DY63657
2597
7291
524
86.9
globlastp

6_P1

LAB669_H34
lettuce|10v1|DW070893
2598
7292
524
86.87
glotblastn

LAB669_H35
amsonia|11v1|SRR098688X1
2599
7293
524
86.8
globlastp

04922_P1

LAB669_H36
apple|11v1|CN496452_P1
2600
7294
524
86.8
globlastp

LAB669_H37
chickpea|11v1|FE670490
2601
7295
524
86.8
globlastp

LAB669_H38
eschscholzia|11v1|CD478433
2602
7296
524
86.8
globlastp

_P1

LAB669_H39
gnetum|10v1|SRR064399S00
2603
7297
524
86.76
glotblastn

52953_T1

LAB669_H97
nicotiana_benthamiana|12v1|
2604
7298
524
86.7
globlastp

EB447116_P1

LAB669_H40
ambrosial|11v1|SRR346935.1
2605
7299
524
86.7
globlastp

53110_P1

LAB669_H41
arnica|11v1|SRR099034X133
2606
7300
524
86.7
globlastp

722_P1

LAB669_H42
prunus|10v1|CN496452
2607
7301
524
86.7
globlastp

LAB669_H43
thellungiella_halophilum|11v
2608
7302
524
86.7
globlastp

1|BY816163

LAB669_H98
aquilegia|10v2|DR919918_P1
2609
7303
524
86.6
globlastp

LAB669_H44
arabidopsis|10v1|AT2G47250
2610
7304
524
86.6
globlastp

_P1

LAB669_H45
b_rapa|11v1|CD824590_P1
2611
7305
524
86.6
globlastp

LAB669_H46
sunflower|12v1|CX944665
2612
7306
524
86.6
globlastp

LAB669_H47
vinca|11v1|SRR098690X1019
2613
7307
524
86.59
glotblastn

11

LAB669_H99
aquilegia|10v2|DR919468_P1
2614
7308
524
86.5
globlastp

LAB669_H48
apple|11v1|MDCRP102529_P
2615
7309
524
86.4
globlastp

1

LAB669_H49
euonymus|11v1|SRR070038X
2616
7310
524
86.4
globlastp

102350_P1

LAB669_H50
poplar|10v1|BI123886
2617
7311
524
86.4
globlastp

LAB669_H51
ambrosia|11v1|SRR346935.1
2618
7312
524
86.26
glotblastn

07427_T1

LAB669_H10
pepper|12v1|CA513944_P1
2619
7313
524
86.2
globlastp

0

LAB669_H52
arabidopsis|10v1|AT3G62310
2620
7314
524
86.2
globlastp

_P1

LAB669_H53
canola|11v1|CN737393_P1
2621
7315
524
86.2
globlastp

LAB669_H54
canola|11v1|DY002306_P1
2622
7316
524
86.2
globlastp

LAB669_H56
cassava|09v1|DB920266_P1
2623
7317
524
86.1
globlastp

LAB669_H57
chelidonium|11v1|SRR08475
2624
7318
524
86.03
glotblastn

2X109346_T1

LAB669_H58
strawberry|11v1|DY671908
2625
7319
524
86
globlastp

LAB669_H59
silene|11v1|SRR096785X101
2626
7320
524
85.9
globlastp

082

LAB669_H60
valeriana|11v1|SRR099039X1
2627
7321
524
85.8
globlastp

02700

LAB669_H61
amorphophallus|11v2|SRR08
2628
7322
524
85.7
globlastp

9351X130574_P1

LAB669_H62
maritime_pine|10v1|BX25500
2629
7323
524
85.7
globlastp

0_P1

LAB669_H63
pine|10v2|BX255000_P1
2630
7324
524
85.6
globlastp

LAB669_H64
poplar|10v1|BI068513
2631
7325
524
85.6
globlastp

LAB669_H65
centaurea|gb166|EL932094_T
2632
7326
524
85.56
glotblastn

1

LAB669_H66
b_rapa|11v1|CX193394_P1
2633
7327
524
85.4
globlastp

LAB669_H67
pseudotsuga|10v1|SRR06511
2634
7328
524
85.3
globlastp

9S0006982

LAB669_H68
canola|11v1|EE440040_P1
2635
7329
524
85.2
globlastp

LAB669_H69
monkeyflower|10v1|GO94485
2636
7330
524
85.1
globlastp

1

LAB669_H69
monkeyflower|12v1|GO94485
2637
7330
524
85.1
globlastp

2_P1

LAB669_H70
euphorbia|11v1|SRR098678X
2638
7331
524
84.8
globlastp

114200_P1

LAB669_H71
physcomitrella|10v1|AW5988
2639
7332
524
84.8
globlastp

57_P1

LAB669_H72
physcomitrella|10v1|BJ17236
2640
7332
524
84.8
globlastp

5_P1

LAB669_H73
plantago|11v2|SRR066373X1
2641
7333
524
84.8
globlastp

22098_P1

LAB669_H74
abies|11v2|SRR098676X1047
2642
7334
524
84.7
globlastp

33_P1

LAB669_H75
ceratodon|10v1|SRR074890S
2643
7335
524
84.7
globlastp

0001291_P1

LAB669_H76
physcomitrella|10v1|BJ60615
2644
7336
524
84.7
globlastp

8_P1

LAB669_H77
flaveria|11v1|SRR149229.164
2645
7337
524
83.8
globlastp

120_P1

LAB669_H78
aristolochia|10v1|FD749754_—
2646
7338
524
83.62
glotblastn

T1

LAB669_H79
lotus|09v1|AV424955_P1
2647
7339
524
83.2
globlastp

LAB669_H80
oak|10v1|DN949716_T1
2648
7340
524
83.09
glotblastn

LAB669_H81
solanum_phureja|09v1|SPHB
2649
7341
524
82.79
glotblastn

G125221

LAB669_H82
tomato|11v1|BG125221
2650
7342
524
82.65
glotblastn

LAB669_H83
tabernaemontana|11v1|SRR09
2651
7343
524
81.89
glotblastn

8689X111497XX1

LAB669_H10
barley|12v1|BF625487_T1
2652
7344
524
81.68
glotblastn

1

LAB669_H84
pseudotsuga|10v1|SRR06511
2653
7345
524
81.63
glotblastn

9S0089962

LAB669_H85
radish|gb164|EV546238
2654
7346
524
81.32
glotblastn

LAB669_H86
podocarpus|10v1|SRR065014
2655
7347
524
80.43
glotblastn

S0008929_T1

LAB669_H87
pine|10v2|CF668741_P1
2656
7348
524
80.2
globlastp

LAB669_H88
cichorium|gb171|DT212456_—
2657
7349
524
80.08
glotblastn

T1

LAB670_H24
switchgrass|12v1|FL727682_—
2658
7350
525
97.9
globlastp

P1

LAB670_H1
sugarcane|10v1|CA154508
2659
7350
525
97.9
globlastp

LAB670_H2
switchgrass|12v1|FE654834_—
2660
7350
525
97.9
globlastp

P1

LAB670_H2
switchgrass|gb167|FE654834
2661
7350
525
97.9
globlastp

LAB670_H3
millet|10v1|EVO454PM0126
2662
7351
525
96.9
globlastp

40_P1

LAB670_H4
sorghum|12v1|SB01G034530
2663
7352
525
96.9
globlastp

LAB670_H5
maize|10v1|AI738313_P1
2664
7353
525
94.8
globlastp

LAB670_H6
cynodon|10v1|ES306689_P1
2665
7354
525
89.7
globlastp

LAB670_H7
rice|11v1|AU092825
2666
7355
525
88.8
globlastp

LAB670_H8
oat|11v1|GO584324_P1
2667
7356
525
88.7
globlastp

LAB670_H9
lolium|10v1|AU245997_P1
2668
7357
525
86.6
globlastp

LAB670_H10
cenchrus|gb166|EB671644_P
2669
7358
525
85.7
globlastp

1

LAB670_H11
cynodon|10v1|ES292573_P1
2670
7359
525
84.7
globlastp

LAB670_H12
foxtail_millet|11v3|PHY7SI0
2671
7360
525
84.7
globlastp

31250M_P1

LAB670_H13
rice|11v1|AF009413
2672
7361
525
84.7
globlastp

LAB670_H14
switchgrass|gb167|FL698409
2673
7362
525
84.7
globlastp

LAB670_H15
switchgrass|gb167|FL701923
2674
7363
525
84.7
globlastp

LAB670_H14,
switchgrass|12v1|FL698408_—
2675
7362
525
84.7
globlastp

LAB670_H15
P1

LAB670_H16
millet|10v1|CD725161_T1
2676
7364
525
84.69
glotblastn

LAB670_H17
lovegrass|gb167|EH192478_P
2677
7365
525
83.7
globlastp

1

LAB670_H18
maize|10v1|W21683_P1
2678
7366
525
83.7
globlastp

LAB670_H19
sorghum|12v1|SB02G040870
2679
7366
525
83.7
globlastp

LAB670_H20
sugarcane|10v1|CA071951
2680
7366
525
83.7
globlastp

LAB670_H21
brachypodium|12v1|BRDI1
2681
7367
525
82
globlastp

G19860_P1

LAB670_H22
maize|10v1|AI901988_P1
2682
7368
525
81.6
globlastp

LAB670_H23
maize|10v1|BI361016_P1
2683
7368
525
81.6
globlastp

LAB671_H1
switchgrass|gb167|FL721656
2684
7369
526
86.51
glotblastn

LAB671_H1
switchgrass|12v1|FL721656_—
2685
7370
526
85.9
globlastp

P1

LAB671_H2
sorghum|12v1|SB01G021260
2686
7371
526
84.8
globlastp

LAB671_H3
maize|10v1|AI600834_T1
2687
7372
526
81.13
glotblastn

LAB672_H1
cotton|11v1|CO122741_T1
2688
7373
527
98.63
glotblastn

LAB673_H1
sorghum|12v1|SB06G022000
2689
7374
528
95.5
globlastp

LAB673_H2
foxtail_millet|11v3|PHY7S10
2690
7375
528
90.7
globlastp

11035M_P1

LAB673_H3
rice|11v1|AU166606
2691
7376
528
85.8
globlastp

LAB673_H4
brachypodium|12v1|BRADI5
2692
7377
528
85.4
globlastp

G15167_P1

LAB673_H5
barley|10v2|BF255786
2693
7378
528
85.3
globlastp

LAB673_H6
wheat|12v3|BE490542_P1
2694
7379
528
85.2
globlastp

LAB673_H6
wheat|10v2|BE490542
2695
7380
528
84.5
globlastp

LAB673_H8
switchgrass|12v1|FL789100_—
2696
7381
528
81.78
glotblastn

T1

LAB673_H7
switchgrass|gb167|FL789100
2697
7382
528
81.78
glotblastn

LAB675_H66
switchgrass|12v1|FL870695_—
2698
7383
529
97.6
globlastp

P1

LAB675_H1
switchgrass|gb167|DN151324
2699
7383
529
97.6
globlastp

LAB675_H1
switchgrass|12v1|DN151324_—
2700
7384
529
97.1
globlastp

P1

LAB675_H2
foxtail_millet|11v3|PHY7SI0
2701
7385
529
96.6
globlastp

13034M_P1

LAB675_H3
sorghum|12v1|SB06G020680
2702
7386
529
96.6
globlastp

LAB675_H4
rice|11v1|AU032090
2703
7387
529
89.9
globlastp

LAB675_H5
aristolochia|10v1|SRR039082
2704
7388
529
88.5
globlastp

S0029633_P1

LAB675_H6
brachypodium|12v1|BRADI5
2705
7389
529
88
globlastp

G13910_P1

LAB675_H67
banana|12v1|FL666095_P1
2706
7390
529
87.5
globlastp

LAB675_H7
rose|12v1|BQ104338
2707
7391
529
87.1
globlastp

LAB675_H8
amorphophallus|11v2|SRR34
2708
7392
529
87.02
glotblastn

6502.1025374_T1

LAB675_H68
onion|12v1|CF434926_P1
2709
7393
529
86.5
globlastp

LAB675_H69
poplar|13v1|AI167041_P1
2710
7394
529
86.5
globlastp

LAB675_H9
poplar|10v1|AI167041
2711
7395
529
86.12
glotblastn

LAB675_H70
bean|12v2|CA916584_P1
2712
7396
529
86.1
globlastp

LAB675_H11
grape|11v1|GSVIVT0101032
2713
7397
529
86.1
globlastp

5001_P1

LAB675_H12
soybean|11v1|GLYMA11G37
2714
7398
529
86.1
globlastp

380

LAB675_H12
soybean|12v1|GLYMA11G37
2715
7398
529
86.1
globlastp

380_P1

LAB675_H13
soybean|11v1|GLYMA18G01
2716
7399
529
86.1
globlastp

350

LAB675_H13
soybean|12v1|GLYMA18G01
2717
7399
529
86.1
globlastp

350_P1

LAB675_H21
chickpea|13v2|SRR133517.12
2718
7400
529
86.1
globlastp

982_P1

LAB675_H14
cacao|10v1|CU591694_P1
2719
7401
529
85.6
globlastp

LAB675_H15
cassava|09v1|JGICASSAVA1
2720
7402
529
85.6
globlastp

1842VALIDM1_P1

LAB675_H16
clementine|11v1|CV718826_—
2721
7403
529
85.6
globlastp

P1

LAB675_H17
cotton|11v1|BF270786XX1_P
2722
7404
529
85.6
globlastp

1

LAB675_H18
gossypium_raimondii|12v1|B
2723
7404
529
85.6
globlastp

F270786_P1

LAB675_H19
orange|11v1|CV718826_P1
2724
7403
529
85.6
globlastp

LAB675_H20
pigeonpea|11v1|SRR054580X
2725
7405
529
85.6
globlastp

104885_P1

LAB675_H21
chickpea|11vl|SRR133520.19
2726
7406
529
85.58
glotblastn

1624

LAB675_H22
b_rapa|11v1|CX189658_P1
2727
7407
529
85.2
globlastp

LAB675_H23
canola|11v1|EE453892_P1
2728
7408
529
85.2
globlastp

LAB675_H24
canola|11v1|EE465570_P1
2729
7407
529
85.2
globlastp

LAB675_H25
ipomoea_nil|10v1|CJ737822_—
2730
7409
529
85.2
globlastp

P1

LAB675_H71
olea|13v1|SRR596247X17347
2731
7410
529
85.1
glotblastn

2D1_T1

LAB675_H72
sesame|12v1|SESI12V139738
2732
7411
529
85.1
globlastp

1_P1

LAB675_H26
barley|10v2|BG365552
2733
7412
529
85.1
globlastp

LAB675_H26
barley|12v1|BG365552_P1
2734
7412
529
85.1
globlastp

LAB675_H27
centaurea|gb166|EH729047_P
2735
7413
529
85.1
globlastp

1

LAB675_H28
cotton|11v1|DR463033_P1
2736
7414
529
85.1
globlastp

LAB675_H29
gossypium_raimondii|12v1|D
2737
7415
529
85.1
globlastp

R463033_P1

LAB675_H30
medicago|12v1|AL371889_P1
2738
7416
529
85.1
globlastp

LAB675_H31
oak|10v1|FN721100_P1
2739
7417
529
85.1
globlastp

LAB675_H32
ambrosial|11v1|SRR346935.3
2740
7418
529
84.6
globlastp

40220_P1

LAB675_H33
apple|11v1|MDP0000778575
2741
7419
529
84.6
globlastp

_P1

LAB675_H34
castorbean|12v1|XM_002514
2742
7420
529
84.6
globlastp

921_P1

LAB675_H35
cynara|gb167|GE599973_P1
2743
7421
529
84.6
globlastp

LAB675_H36
rye|12v1|DRR001012.207137
2744
7422
529
84.6
globlastp

LAB675_H37
sunflower|12v1|DY92149
2745
7423
529
84.6
globlastp

LAB675_H38
sunflower|12v1|EE627136
2746
7423
529
84.6
globlastp

LAB675_H39
trigonella|11v1|SRR066194X
2747
7424
529
84.6
globlastp

204719

LAB675_H40
wheat|10v2|CA643743
2748
7425
529
84.6
globlastp

LAB675_H73
pepper|12v1|CA525786_P1
2749
7426
529
84.2
globlastp

LAB675_H41
sunflower|12v1|EL488921
2750
7427
529
84.13
glotblastn

LAB675_H74
blueberry|12v1|SRR353283X
2751
7428
529
84.1
globlastp

44451D1_P1

LAB675_H40
wheat|12v3|BE404886_P1
2752
7429
529
84.1
globlastp

LAB675_H42
dandelion|10v1|DR402042_P
2753
7430
529
84.1
globlastp

1

LAB675_H43
monkeyflower|10v1|DV20671
2754
7431
529
84.1
globlastp

7

LAB675_H43
monkeyflower|12v1|DV20671
2755
7431
529
84.1
globlastp

7_P1

LAB675_H44
poplar|10v1|BI135599
2756
7432
529
84.1
globlastp

LAB675_H44
poplar|13v1|BI135599_P1
2757
7432
529
84.1
globlastp

LAB675_H45
safflower|gb162|EL408381
2758
7433
529
84.1
globlastp

LAB675_H75
lettuce|12v1|DW061226_P1
2759
7434
529
83.7
globlastp

LAB675_H46
eucalyptus|11v2|SRR001658
2760
7435
529
83.7
globlastp

X12855_P1

LAB675_H47
fagopyrum|11v1|SRR063689
2761
7436
529
83.7
globlastp

X117737_P1

LAB675_H48
lettuce|10v1|DW061226
2762
7434
529
83.7
globlastp

LAB675_H49
radish|gb164|EV546146
2763
7437
529
83.7
globlastp

LAB675_H50
radish|gb164|EW731123
2764
7438
529
83.7
globlastp

LAB675_H51
solanum_phureja|09v1|SPHB
2765
7439
529
83.7
globlastp

G135787

LAB675_H52
cotton|11v1|SRR032367.3522
2766
7440
529
83.65
glotblastn

0_T1

LAB675_H53
thellungiella_halophilum|11v
2767
7441
529
83.3
globlastp

1|EHJGI11019829

LAB675_H54
tomato|11v1|BG135787
2768
7442
529
83.3
globlastp

LAB675_H55
prunus|10v1|CN878790
2769
7443
529
83.25
glotblastn

LAB675_H56
cucurbita|11v1|SRR091276X
2770
7444
529
83.2
globlastp

101901_P1

LAB675_H57
amborella|12v3|SRR038637.3
2771
7445
529
82.7
globlastp

55166_P1

LAB675_H58
ambrosia|11v1|SRR346943.1
2772
7446
529
82.69
glotblastn

31332_T1

LAB675_H59
cassava|09v1|JGICASSAVA1
2773
7447
529
82.5
globlastp

5133M1_P1

LAB675_H60
arabidopsis|10v1|AT3G25980
2774
7448
529
82.3
globlastp

_P1

LAB675_H61
beet|12v1|BV12V1534509_P
2775
7449
529
82.3
globlastp

1

LAB675_H62
artemisia|10v1|SRR019254S0
2776
7450
529
82.2
globlastp

211742_P1

LAB675_H63
tobacco|gb162|CV017227
2777
7451
529
81.25
glotblastn

LAB675_H64
cucurbita|11v1|SRR091276X
2778
7452
529
81.2
globlastp

146710_P1

LAB675_H65
melon|10v1|AM722866_P1
2779
7453
529
80.8
globlastp

LAB676_H5
switchgrass|12v1|FE626861_—
2780
7454
530
83.1
globlastp

P1

LAB676_H3
switchgrass|gb167|FE626861
2781
7454
530
83.1
globlastp

LAB677_H1
sorghum|12v1|SB06G018390
2782
7455
531
82.8
globlastp

LAB678_H1
sorghum|12v1|SB03G010630
2783
7456
532
93.8
globlastp

LAB678_H2
foxtail_millet|11v3|PHY7SI0
2784
7457
532
93.5
globlastp

00454M_P1

LAB678_H3
switchgrass|gb167|FE616854
2785
7458
532
91.8
globlastp

LAB678_H4
rice|11v1|AA752999
2786
7459
532
87.6
globlastp

LAB678_H5
brachypodium|12v1|BRADI2
2787
7460
532
87.2
globlastp

G10040_P1

LAB678_H6
rye|12v1|DRR001012.168834
2788
7461
532
87.2
globlastp

LAB678_H7
wheat|10v2|BE637505
2789
7462
532
87.2
globlastp

LAB678_H9
wheat|12v3|CJ588987_P1
2790
7463
532
81.4
globlastp

LAB678_H8
millet|10v1|EVO454PM0191
2791
7464
532
80.06
glotblastn

63_T1

LAB679_H1
sugarcane|10v1|CA116003
2792
7465
533
94
globlastp

LAB679_H2
sorghum|12v1|SB05G006160
2793
7466
533
93
globlastp

LAB679_H3
foxtail_millet|11v3|PHY7SI0
2794
7467
533
89.6
globlastp

26156M_P1

LAB679_H4
millet|10v1|EVO454PM0179
2795
7468
533
88.6
globlastp

29_P1

LAB679_H6
switchgrass|12v1|FE621446_—
2796
7469
533
88.3
globlastp

P1

LAB679_H5
maize|10v1|BM080434_P1
2797
7470
533
88.1
globlastp

LAB679_H11
switchgrass|12v1|FE627552_—
2798
7471
533
87.1
globlastp

P1

LAB679_H6
switchgrass|gb167|FE621446
2799
7472
533
86.6
globlastp

LAB679_H7
barley|10v2|BG299288
2800
7473
533
81
globlastp

LAB679_H8
brachypodium|12v1|BRADI4
2801
7474
533
80.8
globlastp

G23190_P1

LAB679_H12
wheat|12v3|BE400518_P1
2802
7475
533
80.6
globlastp

LAB679_H9
wheat|10v2|BE400518
2803
7476
533
80.6
globlastp

LAB679_H10
wheat|10v2|BE516031
2804
7477
533
80.4
globlastp

LAB679_H9, L
wheat_|12v3|BE516031_P1
2805
7478
533
80.3
globlastp

AB679_H10

LAB680_H7
rice|11v1|BE041040
2806
7479
534
92
globlastp

LAB680_H14
oil_palm|11v1|GH636163XX
2807
7480
534
83.1
globlastp

1_P1

LAB680_H18
banana|12v1|FF562231_P1
2808
7481
534
80.6
globlastp

LAB680_H16
phalaenopsis|11v1|CB034153
2809
7482
534
80.6
globlastp

_P1

LAB680_H17
poppy|11v1|SRR030259.1058
2810
7483
534
80.43
glotblastn

84_T1

LAB681_H1
maize|10v1|AY109311_P1
2811
7484
535
95.8
globlastp

LAB681_H17
switchgrass|12v1|FE607070_—
2812
7485
535
94.2
globlastp

P1

LAB681_H2
cynodon|10v1|ES300487_P1
2813
7486
535
94.2
globlastp

LAB681_H3
switchgrass|gb167|FE607070
2814
7485
535
94.2
globlastp

LAB681_H4
millet|10v1|CD725692_P1
2815
7487
535
93.3
globlastp

LAB681_H5
sugarcane|10v1|CA228389
2816
7488
535
93.3
globlastp

LAB681_H6
foxtail_millet|11v3|EC612789
2817
7489
535
92.5
globlastp

_P1

LAB681_H7
rice|11v1|GFXAC084319X5
2818
7490
535
92.5
globlastp

LAB681_H8
sorghum|12v1|SB01G016440
2819
7491
535
92.5
globlastp

LAB681_H9
barley|10v2|BE422135
2820
7492
535
90.8
globlastp

LAB681_H10
wheat|10v2|BE604188
2821
7493
535
90
globlastp

LAB681_H11
brachypodium|12v1|BRADI1
2822
7494
535
89.2
globlastp

G15760_P1

LAB681_H12
pseudoroegneria|gb167|FF363
2823
7495
535
89.2
globlastp

392

LAB681_H13
rye|12v1|DRR001012.217860
2824
7496
535
89.2
globlastp

LAB681_H14
rye|12v1|DRR001012.272760
2825
7496
535
89.2
globlastp

LAB681_H10,
wheat|12v3|BE604188_P1
2826
7497
535
89.2
globlastp

LAB681_H15

LAB681_H15
wheat|10v2|BF474954
2827
—
535
88.3
globlastp

LAB681_H16
oat|11v1|GR343370_P1
2828
7498
535
87.5
globlastp

LAB682_H1
sorghum|12v1|SB07G002540
2829
7499
536
87.6
globlastp

LAB683_H1
sorghum|12v1|SB07G022480
2830
7500
537
85.4
globlastp

LAB684_H1
sorghum|12v1|SB03G035190
2831
7501
538
97.1
globlastp

LAB684_H2
foxtail_millet|11v3 |PHY7SI0
2832
7502
538
95.4
globlastp

01007M_P1

LAB684_H3
switchgrass|gb167|DN148777
2833
7503
538
94.8
globlastp

LAB684_H4
brachypodium|12v1|BRADI2
2834
7504
538
92.9
globlastp

G50460_P1

LAB684_H5
rice|11v1|BI804968
2835
7505
538
92.9
globlastp

LAB684_H6
wheat|10v2|BE399172
2836
7506
538
92.7
globlastp

LAB684_H6, L
wheat|12v3|BE399172_P1
2837
7506
538
92.7
globlastp

AB684_H8

LAB684_H7
rye|12v1|DRR001012.148862
2838
7507
538
92.6
globlastp

LAB684_H8
wheat|10v2|BE413970
2839
7508
538
92.6
globlastp

LAB684_H9
barley|10v2|BI949795
2840
7509
538
92.2
globlastp

LAB684_H9
barley|12v1|BI949795_P1
2841
7509
538
92.2
globlastp

LAB684_H17
switchgrass|12v1|FE630210_—
2842
7510
538
83.4
globlastp

P1

LAB684_H18
banana|12v1|MAGEN201200
2843
7511
538
82.6
globlastp

6904_P1

LAB684_H19
banana_|12v1|MAGEN201201
2844
7512
538
82.1
globlastp

1409_P1

LAB684_H10
oil_palm|11v1|EL690470_T1
2845
7513
538
81.68
glotblastn

LAB684_H11
phalaenopsis|11v1|SRR12577
2846
7514
538
81.3
globlastp

1.1004404_P1

LAB684_H20
banana|12v1|MAGEN201203
2847
7515
538
81
globlastp

3981_P1

LAB684_H12
aristolochia|10v1|FD763239_—
2848
7516
538
80.9
globlastp

P1

LAB684_H21
banana|12v1|FL658885_P1
2849
7517
538
80.6
globlastp

LAB684_H13
rice|11v1|CA999854
2850
7518
538
80.4
globlastp

LAB684_H22
sesame|12v1|SESI12v137273
2851
7519
538
80.3
globlastp

7_P1

LAB684_H14
cucumber|09v1|DN910773_P
2852
7520
538
80.2
globlastp

1

LAB684_H15
monkeyflower|10v1|SRR0372
2853
7521
538
80
globlastp

27S0022628

LAB684_H15
monkeyflower|12v1|SRR0372
2854
7521
538
80
globlastp

27.112598_P1

LAB684_H16
watermelon|11v1|VMEL0640
2855
7522
538
80
globlastp

9116432216

LAB685_H1
sorghum|12v1|SB05G002630
2856
7523
539
94.2
globlastp

LAB685_H2
foxtail_millet|11v3|PHY7SI0
2857
7524
539
92.5
globlastp

09692M_P1

LAB685_H3
switchgrass|gb167|FL699752
2858
7525
539
92.32
glotblastn

LAB685_H4
foxtail_millet|11v3|PHY7SI0
2859
7526
539
92
globlastp

26158M_P1

LAB685_H3
switchgrass|12v1|FL699752_—
2860
7527
539
91.81
glotblastn

T1

LAB685_H5
rice|11v1|AA750137
2861
7528
539
86.9
globlastp

LAB685_H6
rice|11v1|CA760354
2862
7529
539
86.9
globlastp

LAB685_H7
brachypodium|12v1|BRADI4
2863
7530
539
86.6
globlastp

G43070_P1

LAB685_H10
wheat|12v3|AL816971_P1
2864
7531
539
86.6
globlastp

LAB685_H8
barley|10v2|BG299773
2865
7532
539
86.2
globlastp

LAB685_H8
barley|12v1|BG299773_P1
2866
7532
539
86.2
globlastp

LAB685_H9
rye|12v1|DRR001012.133899
2867
7533
539
85.9
globlastp

LAB685_H10
wheat|10v2|AL816971
2868
7534
539
85.4
globlastp

LAB685_H11
switchgrass|12v1|FL968831_—
2869
7535
539
81.39
glotblastn

T1

LAB686_H1
foxtail_millet|11v3|EC613092
2870
7536
540
84.2
globlastp

_P1

LAB686_H2
switchgrass|12v1|DN142530_—
2871
7537
540
84.2
globlastp

P1

LAB686_H2
switchgrass|gb167|DN142530
2872
7538
540
84.2
globlastp

LAB686_H3
sorghum|12v1|SB10G002370
2873
7539
540
83.9
globlastp

LAB686_H4
switchgrass|12v1|FE652809_—
2874
7540
540
83.3
globlastp

P1

LAB686_H4
switchgrass|gb167|FE652809
2875
7540
540
83.3
globlastp

LAB686_H5
millet|10v1|EVO454PM0101
2876
7541
540
83
globlastp

64_P1

LAB687_H1
foxtail_millet|11v3|SOLX000
2877
7542
541
92.7
globlastp

14586_P1

LAB687_H2
sorghum|12v1|SB09G028160
2878
7543
541
92.7
globlastp

LAB687_H3
sugarcane|10v1|CA094332
2879
7544
541
92.59
glotblastn

LAB687_H6
switchgrass|12v1|FE600848
2880
7545
541
91.5
globlastp

P1

LAB687_H37
switchgrass|12v1|FL779738_—
2881
7546
541
91.4
globlastp

P1

LAB687_H38
switchgrass|12v1|FE600847_—
2882
7547
541
90.2
globlastp

P1

LAB687_H4
rice|11v1|BI811755
2883
7548
541
90.2
globlastp

LAB687_H5
rice|11v1|OSCRP063273
2884
7548
541
90.2
globlastp

LAB687_H6
switchgrass|gb167|FE600848
2885
7549
541
90.2
globlastp

LAB687_H39
switchgrass|12v1|Pv12v1PR
2886
—
541
90.12
glotblastn

D029268_T1

LAB687_H40
switchgrass|12v1|SRR408048
2887
7550
541
87.8
globlastp

.128884_P1

LAB687_H7
rice|11v1|OSCRP038479
2888
7551
541
87.8
globlastp

LAB687_H8
oat|11v1|GR342437_P1
2889
7552
541
87.7
globlastp

LAB687_H9
brachypodium|12v1|BRADI4
2890
7553
541
84.1
globlastp

G19960T2_P1

LAB687_H10
eucalyptus|11v2|CT986128_P
2891
7554
541
84
globlastp

1

LAB687_H11
wheat|10v2|BE493166
2892
7555
541
84
globlastp

LAB687_H11
wheat|12v3|CA677345_P1
2893
7555
541
84
globlastp

LAB687_H41
barley|12v1|BQ460833_P1
2894
7556
541
82.9
globlastp

LAB687_H12
barley|10v2|BQ460833
2895
7556
541
82.9
globlastp

LAB687_H13
cynodon|10v1|ES306345_T1
2896
7557
541
82.72
glotblastn

LAB687_H14
sorghum|12v1|XM_00244068
2897
7558
541
82.72
glotblastn

5

LAB687_H15
bupleurum|11v1|SRR301254.
2898
7559
541
82.7
globlastp

113180_P1

LAB687_H16
papaya|gb165|EX264968_P1
2899
7560
541
82.7
globlastp

LAB687_H17
watermelon|11v1|AM722624
2900
7561
541
82.7
globlastp

LAB687_H18
wheat|10v2|BQ162388
2901
7562
541
82.7
globlastp

LAB687_H19
rye|12v1|DRR001012.180020
2902
7563
541
81.7
globlastp

LAB687_H42
nicotiana_benthamiana|12v1|
2903
7564
541
81.5
globlastp

AM835788_P1

LAB687_H20
beech|11v1|SRR364434.1955
2904
7565
541
81.5
globlastp

83_P1

LAB687_H21
bupleurum|11v1|SRR301254.
2905
7566
541
81.5
globlastp

132912_P1

LAB687_H22
cowpea|12v1|FF385151_P1
2906
7567
541
81.5
globlastp

LAB687_H23
cowpea|gb166|FF400922
2907
7567
541
81.5
globlastp

LAB687_H24
cucurbita|11v1|SRR091276X
2908
7568
541
81.5
globlastp

125323_P1

LAB687_H25
melon|10v1|AM721919_P1
2909
7569
541
81.5
globlastp

LAB687_H26
phyla|11v2|SRR099035X118
2910
7570
541
81.5
globlastp

819_P1

LAB687_H27
poplar|10v1|BU871381
2911
7571
541
81.5
globlastp

LAB687_H27
poplar|13v1|BU871381_P1
2912
7571
541
81.5
globlastp

LAB687_H28
rye|12v1|DRR001012.277585
2913
7572
541
80.5
globlastp

LAB687_H43
nicotiana_benthamiana|12v1|
2914
7573
541
80.2
globlastp

EH370596_P1

LAB687_H29
cucumber|09v1|AM721919_P
2915
7574
541
80.2
globlastp

1

LAB687_H30
hornbeam|12v1|SRR364455.1
2916
7575
541
80.2
globlastp

91699_P1

LAB687_H31
peanut|10v1|GO259629_P1
2917
7576
541
80.2
globlastp

LAB687_H32
pigeonpea|11v1|SRR054580X
2918
7577
541
80.2
globlastp

190183_P1

LAB687_H33
primula|11v1|SRR098679X10
2919
7578
541
80.2
globlastp

3891_P1

LAB687_H34
solanum_phureja|09v1|SPHE
2920
7579
541
80.2
globlastp

S892312

LAB687_H35
tea|10v1|GE651037
2921
7580
541
80.2
globlastp

LAB687_H36
tobacco|gb162|AM835788
2922
7573
541
80.2
globlastp

LAB689_H1
sorghum|12v1|SB02G029060
2923
7581
543
94.6
globlastp

LAB689_H2
sugarcane|10v1|CA108580
2924
7582
543
94.6
globlastp

LAB689_H3
switchgrass|gb167|FE600816
2925
7583
543
89.4
globlastp

LAB689_H4
switchgrass|gb167|FE607114
2926
7584
543
89.4
globlastp

LAB689_H3, L
switchgrass|12v1|FE600816_—
2927
7583
543
89.4
globlastp

AB689_H4
P1

LAB689_H5
wheat|10v2|CA625248
2928
7585
543
88.51
glotblastn

LAB689_H6
foxtail_millet|11v3|PHY7SI0
2929
7586
543
88
globlastp

31396M_P1

LAB689_H7
oat|11v1|CN816454_T1
2930
7587
543
87.16
glotblastn

LAB689_H8
millet|10v1|EVO454PM0020
2931
7588
543
86.1
globlastp

62_P1

LAB689_H11
wheat|12v3|BE415104_P1
2932
7589
543
84.9
globlastp

LAB689_H9
oat|11v1|GO589657_P1
2933
7590
543
84.4
globlastp

LAB689_H10
oat|11v1|GO592864_P1
2934
7591
543
84.4
globlastp

LAB689_H11
wheat|10v2|BE415104
2935
7592
543
84.2
globlastp

LAB689_H12
brachypodium|12v1|BRADI4
2936
7593
543
84.1
globlastp

G34370_P1

LAB689_H13
barley|10v2|AJ228929
2937
7594
543
83.8
globlastp

LAB689_H13
barley|12v1|AJ228929_P1
2938
7594
543
83.8
globlastp

LAB689_H14
pseudoroegneria|gb167|FF341
2939
7595
543
83.8
globlastp

080

LAB689_H15
rye|12v1|DRR001012.133105
2940
7596
543
83.8
globlastp

LAB689_H16
rye|12v1|DRR001012.159530
2941
7597
543
83.1
globlastp

LAB689_H17
rice|11v1|AA752467
2942
7598
543
82.5
globlastp

LAB689_H18
rye|12v1|BE495345
2943
7599
543
81.2
globlastp

LAB690_H1
sugarcane|10v1|CA072415
2944
7600
544
97.2
globlastp

LAB690_H2
sorghum|12v1|SB01G038560
2945
7601
544
96.5
globlastp

LAB690_H3
foxtail_millet|11v3|PHY7SI0
2946
7602
544
94.5
globlastp

35270M_P1

LAB690_H12
switchgrass|12v1|FL697833_—
2947
7603
544
94.1
globlastp

P1

LAB690_H4
millet|10v1|EVO454PM0088
2948
7604
544
93.1
globlastp

72_P1

LAB690_H5
switchgrass|gb167|FE633218
2949
7605
544
92.73
glotblastn

LAB690_H5
switchgrass|12v1|FE633218_—
2950
7606
544
92.7
globlastp

P1

LAB690_H6
rice|11v1|OSU55768
2951
7607
544
89
globlastp

LAB690_H7
oat|11v1|GO589427_P1
2952
7608
544
88.8
globlastp

LAB690_H8
brachypodium|12v1|BRADI1
2953
7609
544
88.6
globlastp

G65630_P1

LAB690_H9
rice|11v1|D82035
2954
7610
544
88.6
globlastp

LAB690_H13
barley|12v1|AV836293_P1
2955
7611
544
88.4
globlastp

LAB690_H14
wheat|12v3|AL808704_P1
2956
7612
544
87.2
globlastp

LAB690_H15
wheat|12v3|BE592020_P1
2957
7612
544
87.2
globlastp

LAB690_H10
wheat|10v2|BE444447
2958
7612
544
87.2
globlastp

LAB690_H16
wheat|12v3|BG263813_P1
2959
7613
544
87
globlastp

LAB690_H11
rye|12v1|DRR001012.102457
2960
7614
544
87
globlastp

LAB692_H1
sorghum|12v1|SB03G043060
2961
7615
546
96.4
globlastp

LAB692_H6
switchgrass|12v1|FE657867_—
2962
7616
546
93.1
globlastp

P1

LAB692_H7
switchgrass|12v1|FL729331_—
2963
7617
546
92.85
glotblastn

T1

LAB692_H2
foxtail_millet|11v3|PHY7SI0
2964
7618
546
92.8
globlastp

00101M_P1

LAB692_H3
rice|11v1|C93430
2965
7619
546
88.9
globlastp

LAB692_H4
brachypodium|12v1|BRADI2
2966
7620
546
88.2
globlastp

G57967_P1

LAB692_H5
barley|12v1|AV833881_P1
2967
7621
546
86.8
globlastp

LAB692_H5
barley|10v2|AV833881
2968
7622
546
86.7
globlastp

LAB693_H1
maize|10v1|AI372139_P1
2969
7623
547
95.6
globlastp

LAB693_H2
sugarcane|10v1|AA644745
2970
7624
547
91.2
globlastp

LAB693_H3
sorghum|12v1|SB04G000960
2971
7625
547
89.7
globlastp

LAB693_H4
sorghum|12v1|SB04G029240
2972
7626
547
88.4
globlastp

LAB693_H5
foxtail_millet|11v3|PHY7SI0
2973
7627
547
88.2
globlastp

03313M_P1

LAB693_H6
switchgrass|gb167|FL779138
2974
7628
547
87.5
globlastp

LAB693_H6, L
switchgrass|12v1|FE635175_—
2975
7628
547
87.5
globlastp

AB693_H10
P1

LAB693_H7
millet|10v1|EVO454PM0015
2976
7629
547
86.8
globlastp

50_P1

LAB693_H8
cynodon|10v1|ES294205_P1
2977
7630
547
86
globlastp

LAB693_H9
lovegrass|gb167|EH185159_P
2978
7631
547
86
globlastp

1

LAB693_H10
switchgrass|gb167|FE635175
2979
7632
547
86
globlastp

LAB693_H11
switchgrass|gb167|FE633314
2980
7633
547
85.9
globlastp

LAB693_H11
switchgrass|12v1|FE633314_—
2981
7634
547
84.6
globlastp

P1

LAB693_H12
foxtail_millet|11v3|PHY7SI0
2982
7635
547
84.4
globlastp

18665M_P1

LAB693_H13
maize|10v1|BQ163241_P1
2983
7636
547
83.7
globlastp

LAB693_H14
lovegrass|gb167|DN480293_P
2984
7637
547
81.6
globlastp

1

LAB693_H15
rice|11v1|AA754449
2985
7638
547
81.6
globlastp

LAB693_H16
rye|12v1|DRR001012.154309
2986
7639
547
80.9
globlastp

LAB693_H17
oat|11v1|GO589172_P1
2987
7640
547
80.7
globlastp

LAB693_H18
pseudoroegneria|gb167|FF359
2988
7641
547
80.1
globlastp

042

LAB693_H19
wheat|10v2|BE424555
2989
7642
547
80.1
globlastp

LAB693_H20
wheat|10v2|BE428937
2990
7641
547
80.1
globlastp

LAB693_H21
wheat|10v2|SRR043334S000
2991
7643
547
80.1
globlastp

1266

LAB693_H19,
wheat|12v3|BE398538_P1
2992
7643
547
80.1
globlastp

LAB693_H20,

LAB693_H21

LAB693_H22
oat|11v1|GO586361_P1
2993
7644
547
80
globlastp

LAB694_H1
foxtail_millet|11v3|PHY7SI0
2994
548
548
100
globlastp

26975M_P1

LAB694_H2
sorghum|12v1|SB05G007040
2995
548
548
100
globlastp

LAB694_H3
sugarcane|10v1|CA112859X
2996
548
548
100
globlastp

X1

LAB694_H19
prunus_mume|13v1|CB82108
2997
7645
548
99.3
globlastp

5
6_P1

LAB694_H4
apple|11v1|CN496750_T1
2998
7646
548
99.3
glotblastn

LAB694_H5
beech|11v1|FR606329_P1
2999
7645
548
99.3
globlastp

LAB694_H6
beet|12v1|Bv12v1528255_P
3000
7647
548
99.3
globlastp

1

LAB694_H7
cacao|10v1|CU472468_P1
3001
7645
548
99.3
globlastp

LAB694_H8
cassava|09v1|JGICASSAVA1
3002
7648
548
99.3
glotblastn

526VALIDM1_T1

LAB694_H9
castorbean|12v1|EE255215_P
3003
7645
548
99.3
globlastp

1

LAB694_H10
chestnut|gb170|SRR006295S0
3004
7645
548
99.3
globlastp

031341_P1

LAB694_H11
euphorbia|11v1|DV122250X
3005
7645
548
99.3
globlastp

X1_P1

LAB694_H12
heritiera|10v1|SRR005794S00
3006
7645
548
99.3
globlastp

02990_P1

LAB694_H13
humulus|11v1|EX516771_P1
3007
7645
548
99.3
globlastp

LAB694_H14
oak|10v1|FP028821_P1
3008
7645
548
99.3
globlastp

LAB694_H15
papaya|gb165|EX259283_P1
3009
7645
548
99.3
globlastp

LAB694_H16
prunus|10v1|CB821086
3010
7645
548
99.3
globlastp

LAB694_H17
spurge|gb161|DV122250
3011
7645
548
99.3
globlastp

LAB694_H18
strawberry|11v1|DY672990
3012
7645
548
99.3
globlastp

LAB694_H19
switchgrass|12v1|DN141012_—
3013
7649
548
99.3
globlastp

P1

LAB694_H19
switchgrass|gb167|DN141012
3014
7649
548
99.3
globlastp

LAB694_H20
switchgrass|gb167|FL953740
3015
7649
548
99.3
globlastp

LAB694_H19
wheat|12v3|BE404291_P1
3016
7650
548
98.6
globlastp

6

LAB694_H21
barley|10v2|BE216724
3017
7650
548
98.6
globlastp

LAB694_H22
brachypodium|12v1|BRADI4
3018
7650
548
98.6
globlastp

G40287_P1

LAB694_H23
cannabis|12v1|JK501983_P1
3019
7651
548
98.6
globlastp

LAB694_H24
chickpea|11v1|SRR133517.19
3020
7652
548
98.6
globlastp

5513

LAB694_H24
chickpea|13v2|SRR133517.10
3021
7652
548
98.6
globlastp

3174_P1

LAB694_H25
clementine|11v1|CF831846_P
3022
7653
548
98.6
globlastp

1

LAB694_H26
cotton|11v1|AY273900XX1_—
3023
7654
548
98.6
globlastp

P1

LAB694_H27
cucumber|09v1|DV634835_P
3024
7655
548
98.6
globlastp

1

LAB694_H28
cucurbita|11v1|FG227008_P1
3025
7655
548
98.6
globlastp

LAB694_H29
euonymus|11v1|SRR070038X
3026
7656
548
98.6
globlastp

168347_P1

LAB694_H30
euonymus|11v1|SRR070038X
3027
7651
548
98.6
globlastp

317309_P1

LAB694_H31
fescue|gb161|DT709312_P1
3028
7650
548
98.6
globlastp

LAB694_H32
gossypium_raimondii|12v1|A
3029
7654
548
98.6
globlastp

Y273900_P1

LAB694_H33
leymus|gb166|EG375011_P1
3030
7650
548
98.6
globlastp

LAB694_H34
lolium|10v1|AU249760_P1
3031
7650
548
98.6
globlastp

LAB694_H35
maize|10v1|CF040831_P1
3032
7657
548
98.6
globlastp

LAB694_H36
medicago|12v1|BF634954_P1
3033
7652
548
98.6
globlastp

LAB694_H37
medicago|12v1|GE352227_P1
3034
7652
548
98.6
globlastp

LAB694_H38
melon|10v1|DV634835_P1
3035
7655
548
98.6
globlastp

LAB694_H39
millet|10v1|EVO454PM0275
3036
7658
548
98.6
globlastp

17_P1

LAB694_H40
oat|11v1|GO595130_P1
3037
7650
548
98.6
globlastp

LAB694_H41
orange|11v1|CF831846_P1
3038
7653
548
98.6
globlastp

LAB694_H42
peanut|10v1|GO258592_P1
3039
7652
548
98.6
globlastp

LAB694_H43
rice|11v1|AF443601
3040
7659
548
98.6
globlastp

LAB694_H44
rye|12v1|DRR001012.134935
3041
7650
548
98.6
globlastp

LAB694_H45
silene|11v1|GH295102
3042
7660
548
98.6
globlastp

LAB694_H46
sorghum|12v1|SB08G006500
3043
7659
548
98.6
globlastp

LAB694_H47
soybean|11v1|GLYMA14G40
3044
7661
548
98.6
globlastp

430

LAB694_H47
soybean|12v1|GLYMA14G40
3045
7661
548
98.6
globlastp

430T2_P1

LAB694_H48
trigonella|11v1|SRR066194X
3046
7652
548
98.6
globlastp

112195

LAB694_H49
watermelon|11v1|DV634835
3047
7655
548
98.6
globlastp

LAB694_H50
wheat|10v2|BE404291
3048
7650
548
98.6
globlastp

LAB694_H51
hevea|10v1|EC608004_T1
3049
7662
548
98.59
glotblastn

LAB694_H19
bean|12v2|CA907762_P1
3050
7663
548
97.9
globlastp

7

LAB694_H52
bean|12v1|CA907762
3051
7663
548
97.9
globlastp

LAB694_H53
brachypodium|12v1|BRADI4
3052
7664
548
97.9
globlastp

G21080_P1

LAB694_H54
cleome_gynandra|10v1|SRR0
3053
7665
548
97.9
globlastp

15532S0022054_P1

LAB694_H55
cleome_spinosa|10v1|SRR015
3054
7665
548
97.9
globlastp

531S0047808_P1

LAB694_H56
cowpea|12v1|FC460339_P1
3055
7663
548
97.9
globlastp

LAB694_H56
cowpea|gb166|FC460339
3056
7663
548
97.9
globlastp

LAB694_H57
cyamopsis|10v1|EG975466_P
3057
7666
548
97.9
globlastp

1

LAB694_H58
cynodon|10v1|ES299836_P1
3058
7667
548
97.9
globlastp

LAB694_H59
foxtail_millet|11v3|PHY7SI0
3059
7668
548
97.9
globlastp

23579M_P1

LAB694_H60
lotus|09v1|LLAV778204_P1
3060
7663
548
97.9
globlastp

LAB694_H61
lovegrass|gb167|EH185358_P
3061
7669
548
97.9
globlastp

1

LAB694_H62
nasturtium|11v1|SRR032558.
3062
7670
548
97.9
globlastp

109441_P1

LAB694_H63
oat|11v1|CN814910_P1
3063
7671
548
97.9
globlastp

LAB694_H64
oil_palm|11v1|EL691369_P1
3064
7672
548
97.9
globlastp

LAB694_H65
oil_palm|11v1|SRR190698.15
3065
7672
548
97.9
globlastp

5291_P1

LAB694_H66
pigeonpea|11v1|SRR054580X
3066
7663
548
97.9
globlastp

111872_P1

LAB694_H67
poplar|10v1|BI120072
3067
7673
548
97.9
globlastp

LAB694_H67
poplar|13v1|BI120072_P1
3068
7673
548
97.9
globlastp

LAB694_H68
poplar|10v1|BU881768
3069
7674
548
97.9
globlastp

LAB694_H68
poplar|13v1|BI129424_P1
3070
7674
548
97.9
globlastp

LAB694_H69
poppy|11v1|SRR030259.1210
3071
7675
548
97.9
globlastp

18_P1

LAB694_H70
rhizophora|10v1|SRR005793S
3072
7676
548
97.9
globlastp

0006666

LAB694_H71
soybean|11v1|GLYMA17G37
3073
7663
548
97.9
globlastp

730

LAB694_H71
soybean|12v1|GLYMA17G37
3074
7663
548
97.9
globlastp

730_P1

LAB694_H72
sugarcane|10v1|CA073458
3075
7677
548
97.9
globlastp

LAB694_H73
switchgrass|gb167|FL770052
3076
7678
548
97.9
globlastp

LAB694_H74
tripterygium|11v1|SRR09867
3077
7679
548
97.9
globlastp

7X129706

LAB694_H19
onion|12v1|SRR073446X114
3078
7680
548
97.2
globlastp

8
144D1_P1

LAB694_H19
zostera|12v1|AM769512_P1
3079
7681
548
97.2
globlastp

9

LAB694_H75
acacia|10v1|FS587854_P1
3080
7682
548
97.2
globlastp

LAB694_H76
aristolochia|10v1|FD751198_—
3081
7681
548
97.2
globlastp

P1

LAB694_H77
coffea|10v1|EE196618_P1
3082
7683
548
97.2
globlastp

LAB694_H78
eschscholzia|11v1|CD478156_—
3083
7684
548
97.2
globlastp

P1

LAB694_H79
eucalyptus|11v2|CT985757_P
3084
7685
548
97.2
globlastp

1

LAB694_H80
fagopyrum|11v1|SRR063689
3085
7686
548
97.2
globlastp

X146746_P1

LAB694_H81
fagopyrum|11v1|SRR063703
3086
7686
548
97.2
globlastp

X148072_P1

LAB694_H82
flax|11v1|GW867762_P1
3087
7687
548
97.2
globlastp

LAB694_H83
flax|11v1|JG021336_P1
3088
7687
548
97.2
globlastp

LAB694_H84
ginger|gb164|DY345753_P1
3089
7688
548
97.2
globlastp

LAB694_H85
ginseng|10v1|GR872664_P1
3090
7683
548
97.2
globlastp

LAB694_H86
monkeyflower|10v1|GO97796
3091
7689
548
97.2
globlastp

3

LAB694_H86
monkeyflower|12v1|GR10773
3092
7689
548
97.2
globlastp

0_P1

LAB694_H87
phalaenopsis|11v1|SRR12577
3093
7690
548
97.2
globlastp

1.1298511_P1

LAB694_H88
plantago|11v2|SRR066373X1
3094
7683
548
97.2
globlastp

54054_P1

LAB694_H89
tabernaemontana|11v1|SRR09
3095
7683
548
97.2
globlastp

8689X119104

LAB694_H90
valeriana|11v1|SRR099039X1
3096
7691
548
97.2
globlastp

2880

LAB694_H91
zostera|10v1|AM769512
3097
7681
548
97.2
globlastp

LAB694_H20
b_juncea|12v1|E6ANDIZ01C
3098
7692
548
96.5
globlastp

0
FKST_P1

LAB694_H20
olea|13v1|SRR592583X12085
3099
7693
548
96.5
globlastp

1
0D1_P1

LAB694_H92
ambrosia|11v1|SRR346935.5
3100
7694
548
96.5
globlastp

01299_P1

LAB694_H93
amorphophallus|11v2|SRR08
3101
7695
548
96.5
globlastp

9351X152278_P1

LAB694_H94
amsonia|11v1|SRR098688X1
3102
7696
548
96.5
globlastp

04811_P1

LAB694_H95
b_juncea|10v2|E6ANDIZ01B
3103
7692
548
96.5
globlastp

SMB1

LAB694_H96
b_juncea|10v2|E6ANDIZ02G
3104
7697
548
96.5
globlastp

YOM9

LAB694_H97
b_oleracea|gb161|AM060664
3105
7692
548
96.5
globlastp

_P1

LAB694_H98
b_rapa|11v1|CD812122_P1
3106
7692
548
96.5
globlastp

LAB694_H99
b_rapa|11v1|CX191685_P1
3107
7697
548
96.5
globlastp

LAB694_H10
banana|10v1|BBS858T3
3108
7698
548
96.5
globlastp

0

LAB694_H10
canola|11v1|CN731514_P1
3109
7692
548
96.5
globlastp

1

LAB694_H10
canola|11v1|DY024877_P1
3110
7692
548
96.5
globlastp

2

LAB694_H10
canola|11v1|EE456290_P1
3111
7697
548
96.5
globlastp

3

LAB694_H10
canola|11v1|EE475817_P1
3112
7697
548
96.5
globlastp

4

LAB694_H10
flaveria|11v1|SRR149229.204
3113
7694
548
96.5
globlastp

5
976_P1

LAB694_H10
grape|11v1|GSVIVT0101878
3114
7699
548
96.5
globlastp

6
5001_P1

LAB694_H10
guizotia|10v1|GE558589_P1
3115
7694
548
96.5
globlastp

7

LAB694_H10
phyla|11v2|SRR099035X105
3116
7693
548
96.5
globlastp

8
10_P1

LAB694_H10
phyla|11v2|SRR099037X116
3117
7700
548
96.5
globlastp

9
576_P1

LAB694_H11
poppy|11v1|SRR030259.1151
3118
7701
548
96.5
globlastp

0
19_P1

LAB694_H11
poppy|11v1|SRR030259.1173
3119
7701
548
96.5
globlastp

1
04_P1

LAB694_H11
poppy|11v1|SRR030259.1198
3120
7701
548
96.5
globlastp

2
27_P1

LAB694_H11
radish|gb164|EV545467
3121
7697
548
96.5
globlastp

3

LAB694_H11
radish|gb164|EW726273
3122
7697
548
96.5
globlastp

4

LAB694_H11
salvia|10v1|SRR014553S0006
3123
7700
548
96.5
globlastp

5
774

LAB694_H11
sarracenia|11v1|SRR192669.1
3124
7702
548
96.5
globlastp

6
84054

LAB694_H11
sunflower|12v1|DY905323
3125
7694
548
96.5
globlastp

7

LAB694_H11
utricularia|11v1|SRR094438.1
3126
7693
548
96.5
globlastp

8
12373

LAB694_H11
vinca|11v1|SRR098690X1160
3127
7703
548
96.5
globlastp

9
75

LAB694_H15
monkeyflower|12v1|SRR0372
3128
7704
548
96.5
globlastp

2
27.142254_P1

LAB694_H20
b_juncea|12v1|E6ANDIZ01B
3129
7705
548
95.8
globlastp

2
SMB1_P1

LAB694_H20
lettuce|12v1|DW051620_P1
3130
7706
548
95.8
globlastp

3

LAB694_H20
nicotiana_benthamiana|12v1|
3131
7707
548
95.8
globlastp

4
EB678422_P1

LAB694_H20
olea|13v1|SRR014463X14404
3132
7708
548
95.8
globlastp

5
D1_P1

LAB694_H12
amborella|12v2|FD434153
3133
7709
548
95.8
globlastp

0

LAB694_H12
amborella|12v3|FD431880_P
3134
7709
548
95.8
globlastp

0
1

LAB694_H12
artemisia|10v1|EY036010_P1
3135
7706
548
95.8
globlastp

1

LAB694_H12
centaurea|gb166|EH740157_P
3136
7706
548
95.8
globlastp

2
1

LAB694_H12
centaurea|gb166|EH763796_P
3137
7706
548
95.8
globlastp

3
1

LAB694_H12
ceratodon|10v1|SRR074890S
3138
7710
548
95.8
globlastp

4
0075823_P1

LAB694_H12
ceratodon|10v1|SRR074890S
3139
7710
548
95.8
globlastp

5
0109969_P1

LAB694_H12
cichorium|gb171|EH707098_—
3140
7706
548
95.8
globlastp

6
P1

LAB694_H12
cirsium|11v1|SRR346952.109
3141
7706
548
95.8
globlastp

7
89_P1

LAB694_H12
cirsium|11v1|SRR346952.114
3142
7706
548
95.8
globlastp

8
0614_P1

LAB694_H12
cynara|gb167|GE586596_P1
3143
7706
548
95.8
globlastp

9

LAB694_H13
eggplant|10v1|FS007058_P1
3144
7707
548
95.8
globlastp

0

LAB694_H13
fraxinus|11v1|FR644488_P1
3145
7711
548
95.8
globlastp

1

LAB694_H13
gerbera|09v1|AJ750857_P1
3146
7712
548
95.8
globlastp

2

LAB694_H13
ipomoea_batatas|10v1|EE876
3147
7713
548
95.8
globlastp

3
602_P1

LAB694_H13
ipomoea_nil|10v1|CJ737915_—
3148
7713
548
95.8
globlastp

4
P1

LAB694_H13
kiwi|gb166|FG409618_P1
3149
7714
548
95.8
globlastp

5

LAB694_H13
lettuce|10v1|DW051620
3150
7706
548
95.8
globlastp

6

LAB694_H13
marchantia|gb166|BJ847803_—
3151
7715
548
95.8
globlastp

7
P1

LAB694_H13
orobanche|10v1|SRR023189S
3152
7716
548
95.8
globlastp

8
0022046_P1

LAB694_H13
pepper|gb171|CA526095
3153
7707
548
95.8
globlastp

9

LAB694_H14
physcomitrella|10v1|BI43667
3154
7717
548
95.8
globlastp

0
7_P1

LAB694_H14
radish|gb164|EV537666
3155
7718
548
95.8
globlastp

1

LAB694_H14
radish|gb164|EX894968
3156
7718
548
95.8
globlastp

2

LAB694_H14
radish|gb164|EX905361
3157
7718
548
95.8
globlastp

3

LAB694_H14
safflower|gb162|EL407116
3158
7706
548
95.8
globlastp

4

LAB694_H14
solanum_phureja|09v1|SPHA
3159
7707
548
95.8
globlastp

5
W035089

LAB694_H14
tobacco|gb162|EB678422
3160
7707
548
95.8
globlastp

6

LAB694_H14
tomato|11v1|AW035089
3161
7707
548
95.8
globlastp

7

LAB694_H14
tragopogon|10v1|SRR020205
3162
7706
548
95.8
globlastp

8
S0010100

LAB694_H14
triphysaria|10v1|EY009345
3163
7719
548
95.8
globlastp

9

LAB694_H20
onion|12v1|SRR073446X122
3164
7720
548
95.77
glotblastn

6
006D1_T1

LAB694_H15
antirrhinum|gb166|AJ800387
3165
—
548
95.77
glotblastn

0
_T1

LAB694_H15
bupleurum|11v1|SRR301254.
3166
—
548
95.77
glotblastn

1
101222_T1

LAB694_H15
monkeyflower|10v1|SRR0372
3167
—
548
95.77
glotblastn

2
27S0060648

LAB694_H15
monkeyflower|12v1|SRR0372
3168
—
548
95.77
glotblastn

2
27.142254_T1

LAB694_H15
cassava|09v1|JGICASSAVA1
3169
7721
548
95.2
globlastp

3
8156VALIDM1_P1

LAB694_H20
banana|12v1|BBS858T3_P1
3170
7722
548
95.1
globlastp

7

LAB694_H20
nicotiana_benthamiana|12v1|
3171
7723
548
95.1
globlastp

8
AM810338_P1

LAB694_H15
arabidopsis_lyrata|09v1|JGIA
3172
7724
548
95.1
globlastp

4
L004125_P1

LAB694_H15
arabidopsis|10v1|AT1G47830
3173
7724
548
95.1
globlastp

5
_P1

LAB694_H15
cryptomeria|gb166|BP175884
3174
7725
548
95.1
globlastp

6
_P1

LAB694_H15
physcomitrella|10v1|BQ0418
3175
7726
548
95.1
globlastp

7
34_P1

LAB694_H15
radish|gb164|EY911232
3176
7727
548
95.1
globlastp

8

LAB694_H15
valeriana|11v1|SRR099039X1
3177
7728
548
95.1
globlastp

9
02186

LAB694_H16
primula|11v1|SRR098679X11
3178
7729
548
95.07
glotblastn

0
5451_T1

LAB694_H16
sarracenia|11v1|SRR192669.1
3179
7730
548
95.07
glotblastn

1
20878

LAB694_H20
blueberry|12v1|SRR353282X
3180
—
548
95.07
glotblastn

9
35870D1_T1

LAB694_H16
bupleurum|11v1|SRR301254.
3181
—
548
95.07
glotblastn

2
134612_T1

LAB694_H16
gnetum|10v1|SRR064399S00
3182
7731
548
94.4
globlastp

3
06898_P1

LAB694_H16
maritime_pine|10v1|SRR0733
3183
7732
548
94.4
globlastp

4
17S0017091_P1

LAB694_H16
petunia|gb171|FN003954_P1
3184
7733
548
94.4
globlastp

5

LAB694_H16
pine|10v2|AI813210_P1
3185
7732
548
94.4
globlastp

6

LAB694_H16
pseudotsuga|10v1|SRR06511
3186
7732
548
94.4
globlastp

7
9S0008047

LAB694_H16
spikemoss|gb165|FE483659
3187
7734
548
94.4
globlastp

8

LAB694_H16
spruce|11v1|CO239102
3188
7732
548
94.4
globlastp

9

LAB694_H17
spruce|11v1|ES857773
3189
7732
548
94.4
globlastp

0

LAB694_H21
wheat|12v3|CA686278_P1
3190
7735
548
93.7
globlastp

0

LAB694_H17
abies|11v2|SRR098676X1085
3191
7736
548
93.7
globlastp

1
35_P1

LAB694_H17
podocarpus|10v1|SRR065014
3192
7737
548
93.7
globlastp

2
S0127784_P1

LAB694_H17
pteridium|11v1|SRR043594X
3193
7738
548
93.7
globlastp

3
135194

LAB694_H17
sarracenia|11v1|SRR192669.1
3194
7739
548
93.7
globlastp

4
12327

LAB694_H17
spikemoss|gb165|DN838907
3195
7740
548
93.7
globlastp

5

LAB694_H17
platanus|11v1|SRR096786X1
3196
7741
548
91.55
glotblastn

6
12983_T1

LAB694_H21
nicotiana_benthamiana|12v1|
3197
7742
548
91.5
globlastp

1
FS393800_P1

LAB694_H17
arnica|11v1|SRR099034X100
3198
7743
548
91.5
globlastp

7
13_P1

LAB694_H17
momordica|10v1|SRR071315
3199
7744
548
91.2
globlastp

8
S0031575_P1

LAB694_H17
thellungiella_halophilum|11v
3200
7745
548
91.2
globlastp

9
1|BY815295

LAB694_H18
thellungiella_halophilum|11v
3201
7746
548
90.6
globlastp

0
1|EHJGI11028290

LAB694_H18
avocado|10v1|CO997229_P1
3202
7747
548
90.1
globlastp

1

LAB694_H18
peanut|10v1|ES720451_P1
3203
7748
548
90.1
globlastp

2

LAB694_H18
wheat|10v2|CA619060
3204
7749
548
88.89
glotblastn

3

LAB694_H18
sequoia|10v1|SRR065044S00
3205
7750
548
88.73
glotblastn

4
10927

LAB694_H18
grape|11v1|EC946505_P1
3206
7751
548
88
globlastp

5

LAB694_H18
curcuma|10v1|DY384636_P1
3207
7752
548
87.3
globlastp

6

LAB694_H18
fern|gb171|DK954821_P1
3208
7753
548
87.3
globlastp

7

LAB694_H18
fescue|gb161|DT714279_P1
3209
7754
548
85.9
globlastp

8

LAB694_H18
cephalotaxus|11v1|SRR06439
3210
7755
548
84.8
globlastp

9
5X245354_P1

LAB694_H19
canola|11v1|EV010615_P1
3211
7756
548
84.5
globlastp

0

LAB694_H19
catharanthus|11v1|EG562525
3212
7757
548
83.9
globlastp

1
_P1

LAB694_H19
cichorium|gb171|EH710523_—
3213
—
548
83.8
glotblastn

2
T1

LAB694_H19
phyla|11v2|SRR099037X132
3214
7758
548
82.4
globlastp

3
061_P1

LAB694_H19
dandelion|10v1|DY827100_T
3215
—
548
81.29
glotblastn

4
1

LAB695_H1
sorghum|12v1|SB04G006540
3216
7759
549
92.7
globlastp

LAB695_H2
sugarcane|10v1|CA100382
3217
7760
549
91.6
globlastp

LAB695_H3
switchgrass|gb167|FL701429
3218
7761
549
90.1
globlastp

LAB695_H4
maize|10v1|CB924276_P1
3219
7762
549
89.3
globlastp

LAB695_H3
switchgrass|12v1|FL701429_—
3220
7763
549
88.8
globlastp

P1

LAB695_H5
foxtail_millet|11v3|PHY7SI0
3221
7764
549
86.9
globlastp

17983M_P1

LAB695_H11
switchgrass|12v1|FL924134_—
3222
7765
549
86.3
globlastp

P1

LAB695_H6
rice|11v1|AU166398
3223
7766
549
83.2
globlastp

LAB695_H7
brachypodium|12v1|BRADI3
3224
7767
549
82.2
globlastp

G07070_P1

LAB695_H8
millet|10v1|EVO454PM1271
3225
7768
549
81.7
globlastp

47_P1

LAB695_H9
rye|12v1|DRR001012.111811
3226
7769
549
81.1
globlastp

LAB695_H10
wheat|10v2|BE423238
3227
7770
549
81
globlastp

LAB695_H10
wheat|12v3|BE423238_P1
3228
7771
549
80.6
globlastp

LAB696_H1
sorghum|12v1|SB06G017320
3229
7772
550
95.6
globlastp

LAB696_H2
foxtail_millet|11v3|PHY7SI0
3230
7773
550
91.8
globlastp

10524M_P1

LAB696_H3
millet|10v1|EVO454PM0324
3231
7774
550
90.6
globlastp

52_P1

LAB696_H4
leymus|gb166|EG381869_P1
3232
7775
550
84.1
globlastp

LAB696_H5
brachypodium|12v1|BRADI5
3233
7776
550
83.8
globlastp

G10730_P1

LAB696_H6
rice|11v1|AU064635
3234
7777
550
83.5
globlastp

LAB696_H7
barley|10v2|BM817310
3235
7778
550
83.2
globlastp

LAB696_H7
barley|12v1|BM817310_P1
3236
7778
550
83.2
globlastp

LAB696_H8, L
wheat|12v3|AL821258_P1
3237
7779
550
83.2
globlastp

AB696_H10

LAB696_H11
switchgrass|12v1|SRR187765
3238
7780
550
83.09
glotblastn

.633226_T1

LAB696_H8
wheat|10v2|AL821258
3239
7781
550
82.9
globlastp

LAB696_H9
rye|12v1|BE494540
3240
7782
550
82.6
globlastp

LAB696_H10
wheat|10v2|CA619481
3241
7783
550
81.7
globlastp

LAB697_H1
wheat|10v2|CA617636
3242
7784
551
100
glotblastn

LAB697_H2
lovegrass|gb167|DN481243_P
3243
7785
551
90.7
globlastp

1

LAB697_H3
lovegrass|gb167|DN481668_P
3244
7786
551
87
globlastp

1

LAB697_H4
millet|10v1|EVO454PM0070
3245
7787
551
86
globlastp

51_P1

LAB697_H5
sorghum|12v1|SB04G029780
3246
7788
551
86
globlastp

_P1

LAB697_H6
sugarcane|10v1|CA102094_P
3247
7789
551
86
globlastp

1

LAB697_H7
switchgrass|gb167|DN143428
3248
7790
551
86
globlastp

LAB697_H8
switchgrass|gb167|FE637922
3249
7790
551
86
globlastp

LAB697_H9
foxtail_millet|11v3|EC613006
3250
7791
551
85.96
glotblastn

_T1

LAB698_H1
maize|10v1|BM378106_P1
3251
7792
552
87.6
globlastp

LAB698_H7
switchgrass|12v1|DN142545_—
3252
7793
552
86.6
globlastp

P1

LAB698_H2
foxtail_millet|11v3|PHY7SI0
3253
7794
552
85.8
globlastp

02106M_P1

LAB698_H3
switchgrass|12v1|DN149952_—
3254
7795
552
85.2
globlastp

P1

LAB698_H3
switchgrass|gb167|DN142545
3255
7796
552
85.2
globlastp

LAB698_H4
foxtail_millet|11v3|PHY7SI0
3256
7797
552
83.5
globlastp

02078M_P1

LAB698_H5
brachypodium|12v1|BRADI2
3257
7798
552
80.7
globlastp

G53050_P1

LAB698_H6
brachypodium|12v1|SRR0317
3258
7798
552
80.7
globlastp

96.16539_P1

LAB700_H1
sugarcane|10v1|CA066187
3259
7799
553
91.9
globlastp

LAB700_H2
sorghum|12v1|SB03G006150
3260
7800
553
91.6
globlastp

LAB700_H3
maize|10v1|BG354490_P1
3261
7801
553
89.7
globlastp

LAB700_H4
foxtail_millet|11v3|PHY7SI0
3262
7802
553
83.9
globlastp

00510M_P1

LAB701_H1
sorghum|12v1|SB06G023830
3263
7803
554
95.5
globlastp

LAB701_H2
foxtail_millet|11v3|PHY7SI0
3264
7804
554
91.6
globlastp

09698M_P1

LAB701_H12
switchgrass|12v1|FL718964_—
3265
7805
554
90
glotblastn

T1

LAB701_H3
rice|11v1|BI802373
3266
7806
554
83.7
globlastp

LAB701_H4
wheat|10v2|BE604112
3267
7807
554
82.7
globlastp

LAB701_H5
brachypodium|12v1|BRADI5
3268
7808
554
82.2
globlastp

G16970_P1

LAB701_H6
rye|12v1|BE586784
3269
7809
554
82.2
globlastp

LAB701_H7
switchgrass|12v1|DN149962_—
3270
7810
554
81.9
globlastp

P1

LAB701_H7
switchgrass|gb167|DN149962
3271
7811
554
81.9
globlastp

LAB701_H8
barley|10v2|BI948007
3272
7812
554
81.6
globlastp

LAB701_H9
switchgrass|gb167|FE613267
3273
7813
554
81.2
globlastp

LAB701_H10
rice|11v1|U40143
3274
7814
554
81.1
globlastp

LAB701_H11
foxtail_millet|11v3|PHY7SI0
3275
7815
554
80.9
globlastp

16749M_P1

LAB702_H1
sugarcane|10v1|BQ534855
3276
7816
555
94
globlastp

LAB702_H2
sorghum|12v1|SB05G009740
3277
7817
555
92.3
globlastp

LAB702_H3
switchgrass|gb167|DN145709
3278
7818
555
86.4
globlastp

LAB702_H4
foxtail_millet|11v3|PHY7SI0
3279
7819
555
85.3
globlastp

26693M_P1

LAB702_H5
millet|10v1|EVO454PM0022
3280
7820
555
84.9
globlastp

09_P1

LAB703_H1
sorghum|12v1|SB07G028890
3281
7821
556
95.2
globlastp

LAB703_H2
switchgrass|gb167|FL757721
3282
7822
556
89.5
globlastp

LAB703_H2
switchgrass|12v1|FE639357_—
3283
7823
556
88.9
globlastp

P1

LAB703_H3
switchgrass|gb167|DN144427
3284
7824
556
88.9
globlastp

LAB703_H7
switchgrass|12v1|DN144427_—
3285
7825
556
88.6
globlastp

P1

LAB703_H4
millet|10v1|PMSLX0036944
3286
7826
556
88.3
globlastp

D1_P1

LAB703_H5
lovegrass|gb167|DN480383_P
3287
7827
556
87.1
globlastp

1

LAB703_H6
rice|11v1|AU031650
3288
7828
556
83.8
globlastp

LAB704_H1
sorghum|12v1|SB0012S00600
3289
7829
557
85.7
globlastp

0

LAB705_H1
sorghum|12v1|CF760129
3290
7830
558
94.8
globlastp

LAB705_H2
maize|10v1|CD963129_P1
3291
7831
558
84.5
globlastp

LAB705_H3
cynodon|10v1|ES299804_T1
3292
7832
558
81.03
glotblastn

LAB706_H1
sorghum|12v1|SB03G035650
3293
7833
559
93.1
globlastp

LAB706_H3
switchgrass|12v1|SRR187765
3294
7834
559
91.1
globlastp

.191507_P1

LAB706_H2
foxtail_millet|11v3|PHY7SI0
3295
7835
559
91.1
globlastp

03462M_P1

LAB707_H6
switchgrass|12v1|FL815132_—
3296
7836
560
88.2
globlastp

P1

LAB707_H3
brachypodium|12v1|BRADI2
3297
7837
560
84
globlastp

G44900_P1

LAB707_H5
rye|12v1|DRR001012.123408
3298
7838
560
82.9
globlastp

LAB707_H7
wheat|12v3|SRR073321X191
3299
7839
560
82.3
globlastp

628D1_P1

LAB707_H6
switchgrass|gb167|FL815132
3300
7840
560
81.9
glotblastn

LAB708_H1
sorghum|12v1|SB03G040270
3301
7841
561
90.7
globlastp

LAB708_H2
maize|10v1|CF244321_P1
3302
7842
561
88.6
globlastp

LAB708_H3
foxtail_millet|11v3|PHY7SI0
3303
7843
561
88
globlastp

01075M_P1

LAB709_H1
sugarcane|10v1|CA273032
3304
7844
562
90.9
globlastp

LAB709_H2
sorghum|12v1|SB02G022990
3305
7845
562
87.1
globlastp

T2

LAB709_H3
sorghum|12v1|SB02G022990
3306
7845
562
87.1
globlastp

LAB709_H4
foxtail_millet|11v3|PHY7SI0
3307
7846
562
86.4
globlastp

31494M_P1

LAB709_H5
millet|10v1|EVO454PM0293
3308
7847
562
84.8
globlastp

18_P1

LAB709_H6
switchgrass|gb167|DN147166
3309
7848
562
84.1
globlastp

LAB711_H2
sorghum|12v1|SB06G019730
3310
7849
564
91.7
globlastp

LAB711_H3
foxtail_millet|11v3|PHY7SI0
3311
7850
564
83.3
globlastp

09453M_P1

LAB712_H1
sorghum|12v1|SB03G004180
3312
7851
565
92.8
globlastp

LAB712_H9
switchgrass|12v1|FL747718_—
3313
7852
565
89.6
globlastp

P1

LAB712_H10
switchgrass|12v1|JG812654_—
3314
7853
565
89.3
globlastp

P1

LAB712_H2
foxtail_millet|11v3|PHY7SI0
3315
7854
565
88.7
globlastp

00570M_P1

LAB712_H11
switchgrass|12v1|FL754616_—
3316
7855
565
88.5
globlastp

P1

LAB712_H3
brachypodium|12v1|BRADI2
3317
7856
565
82.9
globlastp

G04690_P1

LAB712_H4
rice|11v1|CB621206
3318
7857
565
81.6
globlastp

LAB712_H5
wheat|10v2|BQ620782
3319
7858
565
81.1
globlastp

LAB712_H6
barley|10v2|BG365756
3320
7859
565
81
globlastp

LAB712_H6
barley|12v1|BJ468992_P1
3321
7859
565
81
globlastp

LAB712_H7
rice|11v1|AU225333
3322
7860
565
81
globlastp

LAB712_H8
rye|12v1|DRR001012.106807
3323
7861
565
80.8
globlastp

LAB712_H12
barley|12v1|AV909159_P1
3324
7862
565
80.7
globlastp

LAB712_H5
wheat|12v3|CA498610_P1
3325
7863
565
80.7
globlastp

LAB712_H13
wheat|12v3|BQ903871_P1
3326
7864
565
80.3
globlastp

LAB712_H14
wheat|12v3|SRR073321X235
3327
7865
565
80.09
glotblastn

771D1_T1

LAB713_H1
foxtail_millet|11v3|PHY7SI0
3328
7866
566
84.9
globlastp

30717M_P1

LAB714_H1
sorghum|12v1|SB02G011890
3329
7867
567
98.4
globlastp

LAB714_H2
sugarcane|10v1|BQ534031
3330
7868
567
98.4
globlastp

LAB714_H3
cenchrus|gb166|BM084717_P
3331
7869
567
95.9
globlastp

1

LAB714_H4
millet|10v1|CD724639_P1
3332
7870
567
95.9
globlastp

LAB714_H5
switchgrass|gb167|DN143551
3333
7871
567
95.9
globlastp

LAB714_H6
foxtail_millet|11v3|GT091003
3334
7872
567
95.6
globlastp

_P1

LAB714_H7
switchgrass|gb167|FE609866
3335
7873
567
95.2
globlastp

LAB714_H5, L
switchgrass|12v1|FE609866_—
3336
7874
567
94.5
globlastp

AB714_H7
P1

LAB714_H8
rice|11v1|U38088
3337
7875
567
93.6
globlastp

LAB714_H9
brachypodium|12v1|BRADI4
3338
7876
567
89.9
globlastp

G38430_P1

LAB714_H10
oat|11v1|GR323343_P1
3339
7877
567
88
globlastp

LAB714_H11
wheat|10v2|BE402383
3340
7878
567
87.6
globlastp

LAB714_H11
wheat|12v3|BE402383_P1
3341
7879
567
87.6
globlastp

LAB714_H12
leymus|gb166|EG374984_P1
3342
7880
567
87.4
globlastp

LAB714_H13
oat|11v1|GR325623_P1
3343
7881
567
87.4
globlastp

LAB714_H15
wheat|12v3|BE398504_P1
3344
7882
567
87.4
globlastp

LAB714_H14
rye|12v1|DRR001012.42071
3345
7883
567
87.2
globlastp

LAB714_H60
wheat|12v3|BE400715_P1
3346
7884
567
86.9
globlastp

LAB714_H15
wheat|10v2|BE398504
3347
7885
567
86.9
globlastp

LAB714_H16
rye|12v1|BE495078
3348
7886
567
86.24
glotblastn

LAB714_H17
barley|12v1|AJ228944_P1
3349
7887
567
85.8
globlastp

LAB714_H17
barley|10v2|AJ228944
3350
7888
567
85.6
globlastp

LAB714_H61
switchgrass|12v1|DN149887_—
3351
7889
567
84.4
globlastp

P1

LAB714_H18
rye|12v1|BE494878
3352
7890
567
84.4
globlastp

LAB714_H19
foxtail_millet|11v3|EC611969
3353
7891
567
84.2
globlastp

_P1

LAB714_H20
millet|10v1|EVO454PM0077
3354
7892
567
84.2
globlastp

22_P1

LAB714_H21
barley|10v2|BE412785
3355
7893
567
84.1
globlastp

LAB714_H21
barley|12v1|BE412785_P1
3356
7893
567
84.1
globlastp

LAB714_H22
rye|12v1|BE636848
3357
7894
567
84.1
globlastp

LAB714_H23
wheat|10v2|BF483187
3358
7895
567
84.1
globlastp

LAB714_H24
sorghum|12v1|SB07G024320
3359
7896
567
83.9
globlastp

LAB714_H25
wheat|10v2|BE404185
3360
7897
567
83.9
globlastp

LAB714_H62
switchgrass|12v1|DN143551_—
3361
7898
567
83.88
glotblastn

T1

LAB714_H26
rice|11v1|BE228256
3362
7899
567
83.7
globlastp

LAB714_H27
switchgrass|gb167|DN142429
3363
7900
567
83.7
globlastp

LAB714_H28
wheat|10v2|BE404223
3364
7901
567
83.7
globlastp

LAB714_H23,
wheat|12v3|BE404185_P1
3365
7901
567
83.7
globlastp

LAB714_H25,

LAB714_H28

LAB714_H29
maize|10v1|AI649518_P1
3366
7902
567
83.4
globlastp

LAB714_H27
switchgrass|12v1|DN142429_—
3367
7903
567
83
globlastp

P1

LAB714_H30
sugarcane|10v1|CA069340
3368
7904
567
82.8
globlastp

LAB714_H63
banana|12v1|BBS72T3_P1
3369
7905
567
82.3
globlastp

LAB714_H31
cassava|09v1|CK645547_P1
3370
7906
567
81.8
globlastp

LAB714_H32
curcuma|10v1|DY391188_P1
3371
7907
567
81.8
globlastp

LAB714_H33
oat|11v1|GO583345_T1
3372
7908
567
81.61
glotblastn

LAB714_H64
banana|12v1|FL648823_P1
3373
7909
567
81.6
globlastp

LAB714_H34
humulus|11v1|EX516702_P1
3374
7910
567
81.6
globlastp

LAB714_H35
cotton|11v1|AI726365_P1
3375
7911
567
81.4
globlastp

LAB714_H36
oil_palm|11v1|EL563741_P1
3376
7912
567
81.4
globlastp

LAB714_H37
brachypodium|12v1|BRADI3
3377
7913
567
81.1
globlastp

G42760_P1

LAB714_H38
cotton|11v1|BE053857_P1
3378
7914
567
81.1
globlastp

LAB714_H39
gossypium_raimondii|12v1|AI
3379
7915
567
81.1
globlastp

726365_P1

LAB714_H40
cannabis|12v1|EW700796_P1
3380
7916
567
80.9
globlastp

LAB714_H41
gossypium_raimondii|12v1|AI
3381
7917
567
80.9
globlastp

054614_P1

LAB714_H42
poplar|10v1|BI122179
3382
7918
567
80.9
globlastp

LAB714_H42
poplar|13v1|BI071680_P1
3383
7918
567
80.9
globlastp

LAB714_H43
cotton|11v1|AI726597_P1
3384
7919
567
80.7
globlastp

LAB714_H44
fraxinus|11v1|SRR058827.10
3385
7920
567
80.69
glotblastn

0723_T1

LAB714_H45
eschscholzia|11v1|CD476791
3386
7921
567
80.5
globlastp

_P1

LAB714_H46
liquorice|gb171|FS268815_P1
3387
7922
567
80.5
globlastp

LAB714_H47
tabernaemontana|11v1|SRR09
3388
7923
567
80.5
globlastp

8689X107321

LAB714_H48
cacao|10v1|CF972720_P1
3389
7924
567
80.2
globlastp

LAB714_H49
cotton|11v1|CO074090_P1
3390
7925
567
80.2
globlastp

LAB714_H50
grape|11v1|GSVIVT0103381
3391
7926
567
80.2
globlastp

5001_P1

LAB714_H51
oak|10v1|CU656124_P1
3392
7927
567
80.2
globlastp

LAB714_H52
olea|11v1|SRR014463.12170
3393
7928
567
80.2
globlastp

LAB714_H52
olea|13v1|SRR014463X12170
3394
7928
567
80.2
globlastp

D1_P1

LAB714_H53
platanus|11v1|SRR096786X1
3395
7929
567
80.2
globlastp

00025_P1

LAB714_H54
amsonia|11v1|SRR098688X1
3396
7930
567
80
globlastp

00870_P1

LAB714_H55
cassava|09v1|DV441055_P1
3397
7931
567
80
globlastp

LAB714_H56
chestnut|gb170|SRR006295S0
3398
7932
567
80
globlastp

005317_P1

LAB714_H57
euonymus|11v1|SRR070038X
3399
7933
567
80
globlastp

115555_P1

LAB714_H58
utricularia|11v1|SRR094438.1
3400
7934
567
80
globlastp

00060

LAB714_H59
valeriana|11v1|SRR099039X1
3401
7935
567
80
globlastp

13898

LAB716_H1
poplar|10v1|BU884744
3402
7936
568
82.5
globlastp

LAB716_H1
poplar|13v1|BU884744_P1
3403
7937
568
82.2
globlastp

LAB717_H1
cacao|10v1|CU532745_P1
3404
7938
569
84.3
globlastp

LAB717_H2
cassava|09v1|DB922354_T1
3405
7939
569
84.16
glotblastn

LAB717_H3
clementine|11v1|CB292561_—
3406
7940
569
84.02
glotblastn

T1

LAB717_H4
eucalyptus|11v2|SRR001658
3407
7941
569
82
globlastp

X126_P1

LAB717_H5
orange|11v1|CB292561_P1
3408
7942
569
82
globlastp

LAB717_H6
tabernaemontana|11v1|SRR09
3409
7943
569
81.8
globlastp

8689X104386

LAB717_H7
grape|11v1|GSVIVT0103647
3410
7944
569
81.7
globlastp

7001_P1

LAB717_H8
cotton|11v1|CO086111_P1
3411
7945
569
81.6
globlastp

LAB717_H9
gossypium_raimondii|12v1|D
3412
7946
569
81.6
globlastp

R460390_P1

LAB717_H10
prunus|10v1|DT003514
3413
7947
569
81.19
glotblastn

LAB717_H15
prunus_mume|13v1|SRR3456
3414
7948
569
80.99
glotblastn

75.22354_T1

LAB717_H11
amsonia|11v1|SRR098688X1
3415
7949
569
80.8
globlastp

32103_P1

LAB717_H12
catharanthus|11v1|SRR09869
3416
7950
569
80.4
glotblastn

1X102623_T1

LAB717_H13
strawberry|11v1|SRR034865S
3417
7951
569
80.2
glotblastn

0023400

LAB717_H14
watermelon|11v1|AM720745
3418
7952
569
80.04
glotblastn

LAB718_H1
poplar|10v1|CA927391
3419
7953
570
86.7
globlastp

LAB718_H1
poplar|13v1|CA927391_P1
3420
7954
570
86.7
globlastp

LAB720_H1
rice|11v1|AA749572
3421
7955
572
84.8
globlastp

LAB720_H2
barley|10v2|BE195688
3422
7956
572
83.3
globlastp

LAB720_H3
pseudoroegneria|gb167|FF340
3423
7957
572
83.1
globlastp

188

LAB720_H4
wheat|10v2|BE490629
3424
7958
572
83.1
globlastp

LAB720_H5
wheat|10v2|BE399464
3425
7959
572
82.7
globlastp

LAB720_H6
rye|12v1|BE494380
3426
7960
572
81.8
globlastp

LAB720_H7
rye|12v1|DRR001012.114722
3427
7961
572
81.8
globlastp

LAB723_H1
sugarcane|10v1|CA148016
3428
7962
575
90.5
globlastp

LAB723_H2
foxtail_millet|11v3|PHY7SI0
3429
7963
575
90.48
glotblastn

25301M_T1

LAB723_H3
switchgrass|12v1|FE647037_—
3430
7964
575
90.2
globlastp

P1

LAB723_H3
switchgrass|gb167|FE647037
3431
7964
575
90.2
globlastp

LAB723_H13
switchgrass|12v1|HO264921_—
3432
7965
575
89.6
globlastp

P1

LAB723_H4
sorghum|12v1|SB09G004990
3433
7966
575
89.4
globlastp

LAB723_H14
wheat|12v3|BE412047_P1
3434
7967
575
88.8
globlastp

LAB723_H5
pseudoroegneria|gb167|FF343
3435
7968
575
88.5
globlastp

540

LAB723_H7
wheat|12v3|BG906361_P1
3436
7969
575
88.5
globlastp

LAB723_H6
rye|12v1|DRR001012.121959
3437
7970
575
88.2
glotblastn

LAB723_H7
wheat|10v2|BE412047
3438
7971
575
88.2
globlastp

LAB723_H15
barley|12v1|BI952425_P1
3439
7972
575
88
globlastp

LAB723_H16
wheat|12v3|CA731684_P1
3440
7973
575
88
globlastp

LAB723_H8
barley|10v2|BI952425
3441
7972
575
88
globlastp

LAB723_H9
oat|11v1|GO594497_P1
3442
7974
575
87.7
globlastp

LAB723_H10
brachypodium|12v1|BRADI2
3443
7975
575
87.4
globlastp

G34490_P1

LAB723_H11
fescue|gb161|DT681092_P1
3444
7976
575
83.6
globlastp

LAB723_H12
maize|10v1|AW129873_T1
3445
7977
575
82.08
glotblastn

LAB726_H37
switchgrass|12v1_DN146436_—
3446
7978
578
94.8
globlastp

2
P1

LAB726_H1
foxtail_millet|11v3|PHY7SI0
3447
7979
578
94.8
globlastp

22299M_P1

LAB726_H2
lovegrass|gb167|EH185063_P
3448
7980
578
94.8
globlastp

1

LAB726_H3
switchgrass|gb167|DN146436
3449
7978
578
94.8
globlastp

LAB726_H4
sorghum|12v1|SB09G030530
3450
7981
578
94.4
globlastp

LAB726_H5
cenchrus|gb166|EB655327_P
3451
7982
578
94.1
globlastp

1

LAB726_H6
sugarcane|10v1|BQ530112
3452
7983
578
94.1
globlastp

LAB726_H7
maize|10v1|BG354255_P1
3453
7984
578
93.4
globlastp

LAB726_H8
brachypodium|12v1|BRADI4
3454
7985
578
93.1
globlastp

G31150T2_P1

LAB726_H9
cucumber|09v1|DV634738_P
3455
7986
578
93.1
globlastp

1

LAB726_H10
switchgrass|gb167|FE600087
3456
7987
578
93.1
globlastp

LAB726_H3, L
switchgrass|12v1|FE600087_—
3457
7987
578
93.1
globlastp

AB726_H10
P1

LAB726_H11
millet|10v1|EVO454PM0238
3458
7988
578
92.71
glotblastn

06_T1

LAB726_H12
melon|10v1|DV634738_P1
3459
7989
578
92.7
globlastp

LAB726_H13
watermelon|11v1|DV634738
3460
7990
578
92.4
globlastp

LAB726_H37
wheat|12v3|BI750581_P1
3461
7991
578
91.7
globlastp

3

LAB726_H37
wheat|12v3|BM135383_P1
3462
7991
578
91.7
globlastp

4

LAB726_H37
wheat|12v3|CJ963454_P1
3463
7991
578
91.7
globlastp

5

LAB726_H14
wheat|10v2|BE402061
3464
7991
578
91.7
globlastp

LAB726_H15
cucurbita|11v1|SRR091276X
3465
7992
578
91.67
glotblastn

101454_T1

LAB726_H16
rye|12v1|DRR001012.100493
3466
7993
578
91.32
glotblastn

LAB726_H17
rye|12v1|DRR001012.163270
3467
7994
578
91.32
glotblastn

LAB726_H18
leymus|gb166|EG377183_P1
3468
7995
578
91.3
globlastp

LAB726_H19
rye|12v1|DRR001012.105610
3469
7996
578
91.3
globlastp

LAB726_H20
rye|12v1|DRR001012.119697
3470
7996
578
91.3
globlastp

LAB726_H37
barley|12v1|BI946680_P1
3471
7997
578
91
globlastp

6

LAB726_H21
barley|10v2|BI946680
3472
7997
578
91
globlastp

LAB726_H21
barley|12v1|BQ458873_P1
3473
7997
578
91
globlastp

LAB726_H22
oat|11v1|GO587381_P1
3474
7998
578
91
globlastp

LAB726_H23
oil_palm|11v1|EL688560_P1
3475
7999
578
91
globlastp

LAB726_H24
papaya|gb165|EF512303_P1
3476
8000
578
91
globlastp

LAB726_H28,
wheat|12v3|BF473864_P1
3477
8001
578
91
globlastp

LAB726_H29

LAB726_H25
rye|12v1|DRR001012.157345
3478
8002
578
90.97
glotblastn

LAB726_H26
catharanthus|11v1|EG557587
3479
8003
578
90.7
globlastp

_P1

LAB726_H27
tripterygium|11v1|SRR09867
3480
8004
578
90.62
glotblastn

7X109403

LAB726_H28
wheat|10v2|BF473864
3481
8005
578
90.6
globlastp

LAB726_H29
wheat|10v2|BQ171535
3482
8006
578
90.6
globlastp

LAB726_H30
tabernaemontana|11v1|SRR09
3483
8007
578
90.3
globlastp

8689X127031

LAB726_H31
cannabis|12v1|GR220740_P1
3484
8008
578
90.1
globlastp

LAB726_H32
cannabis|12v1|SOLX0001290
3485
8008
578
90.1
globlastp

2_P1

LAB726_H33
humulus|11v1|EX516029_P1
3486
8008
578
90.1
globlastp

LAB726_H37
olea|13v1|JK749148_P1
3487
8009
578
90
globlastp

7

LAB726_H34
cotton|11v1|BF270336_P1
3488
8010
578
90
globlastp

LAB726_H35
gossypium_raimondii|12v1|B
3489
8010
578
90
globlastp

F270336_P1

LAB726_H36
rye|12v1|DRR001015.101690
3490
8011
578
89.93
glotblastn

LAB726_H37
antirrhinum|gb166|AJ568109
3491
8012
578
89.7
globlastp

_P1

LAB726_H38
oat|11v1|GR315380_P1
3492
8013
578
89.7
globlastp

LAB726_H39
amsonia|11v1|SRR098688X1
3493
8014
578
89.6
globlastp

11782_P1

LAB726_H40
liriodendron|gb166|CK74336
3494
8015
578
89.6
globlastp

2_P1

LAB726_H41
rye|12v1|DRR001012.118302
3495
8016
578
89.58
glotblastn

LAB726_H42
grape|11v1|GSVIVT0100806
3496
8017
578
89.3
globlastp

0001_P1

LAB726_H37
b_juncea|12v1|E6ANDIZ01C
3497
8018
578
89.2
globlastp

8
ONDG_P1

LAB726_H37
lettuce|12v1|DW045312_P1
3498
8019
578
89.2
globlastp

9

LAB726_H43
b_rapa|11v1|CD813356_P1
3499
8018
578
89.2
globlastp

LAB726_H44
b_rapa|11v1|CD815135_P1
3500
8020
578
89.2
globlastp

LAB726_H45
b_rapa|11v1|CD815732_P1
3501
8018
578
89.2
globlastp

LAB726_H46
canola|11v1|DY005251_P1
3502
8020
578
89.2
globlastp

LAB726_H47
canola|11v1|EE456687_P1
3503
8018
578
89.2
globlastp

LAB726_H48
euonymus|11v1|SRR070038X
3504
8021
578
89.2
globlastp

1252_P1

LAB726_H49
euonymus|11v1|SRR070038X
3505
8022
578
89.2
globlastp

175591_P1

LAB726_H50
lettuce|10v1|DW045312
3506
8019
578
89.2
globlastp

LAB726_H51
olea|11v1|SRR014463.15174
3507
8023
578
89.2
globlastp

LAB726_H52
phalaenopsis|11v1|CB03219
3508
8024
578
89.2
globlastp

5_P1

LAB726_H53
rose|12v1|BQ104032
3509
8025
578
89.2
globlastp

LAB726_H54
strawberry|11v1|EX658465
3510
8026
578
89.2
globlastp

LAB726_H55
thellungiella_parvulum|11v1|
3511
8027
578
89.2
globlastp

DN772889

LAB726_H56
tragopogon|10v1|SRR020205
3512
8028
578
89.2
globlastp

S0024830

LAB726_H38
olea|13v1|SRR014463X14498
3513
8029
578
89
globlastp

0
D1_P1

LAB726_H57
cacao|10v1|CGD0019194_P1
3514
8030
578
89
globlastp

LAB726_H58
rye|12v1|DRR001012.175646
3515
8031
578
88.93
glotblastn

LAB726_H38
b_juncea|12v1|E6ANDIZ01A
3516
8032
578
88.9
globlastp

1
Z57D_P1

LAB726_H59
canola|11v1|EG020885_P1
3517
8033
578
88.9
globlastp

LAB726_H60
chestnut|gb170|SRR006295S0
3518
8034
578
88.9
globlastp

058525_P1

LAB726_H61
cynara|gb167|GE586766_P1
3519
8035
578
88.9
globlastp

LAB726_H62
phalaenopsis|11v1|SRR12577
3520
8036
578
88.9
globlastp

1.1014877_P1

LAB726_H63
prunus|10v1|CN494116
3521
8037
578
88.9
globlastp

LAB726_H64
radish|gb164|EV535720
3522
8038
578
88.9
globlastp

LAB726_H65
radish|gb164|EV544540
3523
8038
578
88.9
globlastp

LAB726_H66
safflower|gb162|EL404368
3524
8039
578
88.9
globlastp

LAB726_H67
sunflower|12v1|DY916997
3525
8040
578
88.9
globlastp

LAB726_H68
coffea|10v1|CF588907_P1
3526
8041
578
88.6
globlastp

LAB726_H69
solanum_phureja|09v1|SPHB
3527
8042
578
88.6
globlastp

G600514

LAB726_H70
walnuts|gb166|CV198105
3528
8043
578
88.6
globlastp

LAB726_H38
aquilegia|10v2|DR914566_P1
3529
8044
578
88.5
globlastp

2

LAB726_H38
prunus_mume|13v1|DT45543
3530
8045
578
88.5
globlastp

3
0_P1

LAB726_H71
aquilegia|10v1|DR914566
3531
8044
578
88.5
globlastp

LAB726_H72
b_oleracea|gb161|DY029722_—
3532
8046
578
88.5
globlastp

P1

LAB726_H73
cacao|10v1|CU485077_P1
3533
8047
578
88.5
globlastp

LAB726_H74
cassava|09v1|CK645417_P1
3534
8048
578
88.5
globlastp

LAB726_H75
cichorium|gb171|EH685765_—
3535
8049
578
88.5
globlastp

P1

LAB726_H76
euonymus|11v1|SRR070038X
3536
8050
578
88.5
globlastp

479018_P1

LAB726_H77
orange|11v1|CV716284_P1
3537
8051
578
88.5
globlastp

LAB726_H78
sunflower|12v1|EE633258
3538
8052
578
88.5
globlastp

LAB726_H79
poppy|11v1|SRR030259.3222
3539
8053
578
88.3
globlastp

79_P1

LAB726_H38
blueberry|12v1|SRR353282X
3540
8054
578
88.2
globlastp

4
100556D1_P1

LAB726_H80
artemisia|10v1|EY037814_P1
3541
8055
578
88.2
globlastp

LAB726_H81
cotton|11v1|BF279079_P1
3542
8056
578
88.2
globlastp

LAB726_H82
flaveria|11v1|SRR149229.101
3543
8057
578
88.2
globlastp

075_P1

LAB726_H83
flaveria|11v1|SRR149229.259
3544
8058
578
88.2
globlastp

124_P1

LAB726_H84
flaveria|11v1|SRR149229.320
3545
8058
578
88.2
globlastp

303_P1

LAB726_H85
gossypium_raimondii|12v1|B
3546
8056
578
88.2
globlastp

F279079_P1

LAB726_H86
radish|gb164|EX777452
3547
8059
578
88.2
globlastp

LAB726_H87
triphysaria|10v1|BM357487
3548
8060
578
88.2
globlastp

LAB726_H88
flaveria|11v1|SRR149229.104
3549
8061
578
88.19
glotblastn

464_T1

LAB726_H89
gossypium_raimondii|12v1|S
3550
8062
578
88.19
glotblastn

RR032881.551349_T1

LAB726_H38
pepper|12v1|AA840647_P1
3551
8063
578
87.9
globlastp

5

LAB726_H90
aristolochia|10v1|FD762042_—
3552
8064
578
87.9
globlastp

P1

LAB726_H91
pepper|gb171|AA840647
3553
8063
578
87.9
globlastp

LAB726_H92
tripterygium|11v1|SRR09867
3554
8065
578
87.9
globlastp

7X105253

LAB726_H38
nicotiana_benthamiana|12v1|
3555
8066
578
87.8
globlastp

6
EG650260_P1

LAB726_H93
arabidopsis_lyrata|09v1|JGIA
3556
8067
578
87.8
globlastp

L030925_P1

LAB726_H94
arnica|11v1|SRR099034X102
3557
8068
578
87.8
globlastp

462_P1

LAB726_H95
clementine|11v1|CO913282_—
3558
8069
578
87.8
globlastp

P1

LAB726_H96
clementine|11v1|CV716284_—
3559
8070
578
87.8
globlastp

P1

LAB726_H97
flaveria|11v1|SRR149229.102
3560
8071
578
87.8
globlastp

383_P1

LAB726_H98
flaveria|11v1|SRR149229.237
3561
8072
578
87.8
globlastp

964_P1

LAB726_H99
flaveria|11v1|SRR149232.131
3562
8073
578
87.8
globlastp

224_P1

LAB726_H10
flaveria|11v1|SRR149232.168
3563
8074
578
87.8
globlastp

0
973_P1

LAB726_H10
ipomoea_nil|10v1|BJ560716_—
3564
8075
578
87.8
globlastp

1
P1

LAB726_H10
oak|10v1|FP054742_P1
3565
8076
578
87.8
globlastp

2

LAB726_H10
orange|11v1|CO913282_P1
3566
8069
578
87.8
globlastp

3

LAB726_H10
sunflower|12v1|DY914832
3567
8077
578
87.8
globlastp

4

LAB726_H10
thellungiella_halophilum|11v
3568
8078
578
87.8
globlastp

5
1|DN772889

LAB726_H13
chickpea|13v2|SRR133517.16
3569
8079
578
87.8
globlastp

1
7801_P1

LAB726_H10
tomato|11v1|AA840647
3570
8080
578
87.6
globlastp

6

LAB726_H38
onion|12v1|CF441482_P1
3571
8081
578
87.5
globlastp

7

LAB726_H10
flaveria|11v1|SRR149229.117
3572
8082
578
87.5
globlastp

7
438_P1

LAB726_H10
flaveria|11v1|SRR149229.141
3573
8083
578
87.5
globlastp

8
013_P1

LAB726_H10
flax|11v1|JG086905_P1
3574
8084
578
87.5
globlastp

9

LAB726_H11
orobanche|10v1|SRR023189S
3575
8085
578
87.5
globlastp

0
0004745_P1

LAB726_H11
peanut|10v1|CX128243_P1
3576
8086
578
87.5
globlastp

1

LAB726_H11
pigeonpea|11v1|SRR054580X
3577
8087
578
87.5
globlastp

2
113696_P1

LAB726_H11
potato|10v1|BG351119_P1
3578
8088
578
87.5
globlastp

3

LAB726_H11
rye|12v1|DRR001012.117176
3579
8089
578
87.5
glotblastn

4

LAB726_H11
solanum_phureja|09v1|SPHB
3580
8088
578
87.5
globlastp

5
G128123

LAB726_H11
triphysaria|10v1|EY134479
3581
8090
578
87.5
globlastp

6

LAB726_H12
poplar|13v1|BI128207_P1
3582
8091
578
87.5
globlastp

3

LAB726_H15
nicotiana_benthamiana|12v1|
3583
8092
578
87.5
globlastp

9
BP749565_P1

LAB726_H11
eschscholzia|11v1|CK746554
3584
8093
578
87.3
globlastp

7
_P1

LAB726_H38
banana|12v1|MAGEN201203
3585
8094
578
87.2
globlastp

8
1235_P1

LAB726_H38
nicotiana_benthamiana|12v1|
3586
8095
578
87.2
globlastp

9
CN498792_P1

LAB726_H39
pepper|12v1|BM064710_P1
3587
8096
578
87.2
globlastp

0

LAB726_H11
arabidopsis|10v1|AT5G62740
3588
8097
578
87.2
globlastp

8
_P1

LAB726_H11
banana|10v1|FF557992
3589
8098
578
87.2
globlastp

9

LAB726_H12
eucalyptus|11v2|ES589326_P
3590
8099
578
87.2
globlastp

0
1

LAB726_H12
oak|10v1|FP053562_P1
3591
8100
578
87.2
globlastp

1

LAB726_H12
pepper|gb171|BM064710
3592
8096
578
87.2
globlastp

2

LAB726_H12
poplar|10v1|CX182839
3593
8101
578
87.2
globlastp

3

LAB726_H12
potato|10v1|BG590188_P1
3594
8102
578
87.2
globlastp

4

LAB726_H12
solanum_phureja|09v1|SPHB
3595
8102
578
87.2
globlastp

5
G128859

LAB726_H12
soybean|11v1|GLYMA17G10
3596
8103
578
87.2
globlastp

6
520

LAB726_H12
soybean|12v1|GLYMA17G10
3597
8103
578
87.2
globlastp

6
520T3_P1

LAB726_H12
sunflower|12v1|DY906726
3598
8104
578
87.2
globlastp

7

LAB726_H12
sunflower|12v1|DY938575
3599
8105
578
87.2
globlastp

8

LAB726_H12
tomato|11v1|BG128859
3600
8106
578
87.2
globlastp

9

LAB726_H13
tomato|11v1|NTU66271
3601
8107
578
87.2
globlastp

0

LAB726_H13
chickpea|11v1|SRR133517.16
3602
8108
578
87.15
glotblastn

1
7801

LAB726_H13
euphorbia|11v1|BP962714_P1
3603
8109
578
86.9
globlastp

2

LAB726_H13
guizotia|10v1|GE555049_T1
3604
8110
578
86.9
glotblastn

3

LAB726_H13
platanus|11v1|SRR096786X1
3605
8111
578
86.9
globlastp

4
09212_P1

LAB726_H39
aquilegia|10v2|DR936172_P1
3606
8112
578
86.8
globlastp

1

LAB726_H39
banana|12v1|FF557992_P1
3607
8113
578
86.8
globlastp

2

LAB726_H39
pepper|12v1|CA514695_P1
3608
8114
578
86.8
globlastp

3

LAB726_H13
ambrosia|11v1|SRR346935.1
3609
8115
578
86.8
globlastp

5
04157_P1

LAB726_H13
aquilegia|10v1|DR936172
3610
8112
578
86.8
globlastp

6

LAB726_H13
cirsium|11v1|SRR346952.100
3611
8116
578
86.8
globlastp

7
1426_P1

LAB726_H13
cirsium|11v1|SRR346952.103
3612
8117
578
86.8
globlastp

8
6905_P1

LAB726_H13
kiwi|gb166|FG406996_P1
3613
8118
578
86.8
globlastp

9

LAB726_H14
oak|10v1|SRR006307S00055
3614
8119
578
86.8
globlastp

0
97_P1

LAB726_H14
pepper|gb171|CA514695
3615
8114
578
86.8
globlastp

1

LAB726_H14
tobacco|gb162|EB444324
3616
8120
578
86.8
globlastp

2

LAB726_H14
valeriana|11v1|SRR099039X1
3617
8121
578
86.8
globlastp

3
01065

LAB726_H39
lettuce|12v1|DW055218_P1
3618
8122
578
86.5
globlastp

4

LAB726_H39
nicotiana_benthamiana|12v1|
3619
8123
578
86.5
globlastp

5
CK294708_P1

LAB726_H14
ambrosia|11v1|SRR346935.1
3620
8124
578
86.5
globlastp

4
81380_P1

LAB726_H14
amorphophallus|11v2|SRR08
3621
8125
578
86.5
globlastp

5
9351X258636_P1

LAB726_H14
artemisia|10v1|EY039911_P1
3622
8126
578
86.5
globlastp

6

LAB726_H14
cichorium|gb171|EH688063_—
3623
8127
578
86.5
globlastp

7
P1

LAB726_H14
lettuce|10v1|DW055218
3624
8128
578
86.5
globlastp

8

LAB726_H14
oil_palm|11v1|EY405316_P1
3625
8129
578
86.5
globlastp

9

LAB726_H39
bean|12v2|SRR001334.19699
3626
8130
578
86.2
globlastp

6
8_P1

LAB726_H15
medicago|12v1|AL367969_P1
3627
8131
578
86.2
globlastp

1

LAB726_H15
medicago|12v1|AW776032_P
3628
8131
578
86.2
globlastp

2
1

LAB726_H15
tobacco|gb162|EB677602
3629
8132
578
86.2
globlastp

3

LAB726_H15
trigonella|11v1|SRR066194X
3630
8133
578
86.2
globlastp

4
138067

LAB726_H15
trigonella|11v1|SRR066194X
3631
8134
578
86.2
globlastp

5
169402

LAB726_H15
cotton|11v1|DT550327_P1
3632
8135
578
86.1
globlastp

6

LAB726_H15
ginger|gb164|DY364165_P1
3633
8136
578
86.1
globlastp

7

LAB726_H15
gossypium_raimondii|12v1|D
3634
8135
578
86.1
globlastp

8
T550327_P1

LAB726_H15
nicotiana_benthamiana|gb162|
3635
8137
578
86.1
globlastp

9
CK287433

LAB726_H16
oil_palm|11v1|ES414433_P1
3636
8138
578
86.1
globlastp

0

LAB726_H16
platanus|11v1|SRR096786X1
3637
8139
578
86.1
globlastp

1
04589_P1

LAB726_H39
nicotiana_benthamiana|12v1|
3638
8140
578
85.9
globlastp

7
EB677602_P1

LAB726_H16
castorbean|11v1|EG657011
3639
8141
578
85.8
globlastp

2

LAB726_H16
castorbean|12v1|EG657011_P
3640
8141
578
85.8
globlastp

2
1

LAB726_H16
chestnut|gb170|SRR006295S0
3641
8142
578
85.8
globlastp

3
004975_P1

LAB726_H16
dandelion|10v1|DR402791_P
3642
8143
578
85.8
globlastp

4
1

LAB726_H16
medicago|12v1|AL384603_P1
3643
8144
578
85.8
globlastp

5

LAB726_H16
oil_palm|11v1|EL694397_P1
3644
8145
578
85.8
globlastp

6

LAB726_H16
poplar|10v1|BI124137
3645
8146
578
85.8
globlastp

7

LAB726_H16
poplar|13v1|BI124137_P1
3646
8146
578
85.8
globlastp

7

LAB726_H16
scabiosa|11v1|SRR063723X1
3647
8147
578
85.8
globlastp

8
20961

LAB726_H16
trigonella|11v1|SRR066194X
3648
8148
578
85.8
globlastp

9
238507

LAB726_H39
onion|12v1|FS209770_T1
3649
8149
578
85.76
glotblastn

8

LAB726_H17
medicago|12v1|AW685772_T
3650
8150
578
85.76
glotblastn

0
1

LAB726_H17
amborella|12v3|CK744765_P
3651
8151
578
85.5
globlastp

1
1

LAB726_H17
catharanthus|11v1|EG559311
3652
8152
578
85.42
glotblastn

2
_T1

LAB726_H39
lettuce|12v1|DW051519_P1
3653
8153
578
85.4
globlastp

9

LAB726_H17
cassava|09v1|BM260131_P1
3654
8154
578
85.4
globlastp

3

LAB726_H17
cirsium|11v1|SRR346952.135
3655
8155
578
85.4
globlastp

4
170_P1

LAB726_H17
lettuce|10v1|DW051519
3656
8153
578
85.4
globlastp

5

LAB726_H17
melon|10v1|AM723504_P1
3657
8156
578
85.4
globlastp

6

LAB726_H17
monkeyflower|10v1|GO96636
3658
8157
578
85.4
globlastp

7
5

LAB726_H17
monkeyflower|12v1|GR00275
3659
8157
578
85.4
globlastp

7
9_P1

LAB726_H17
oak|10v1|CU657562_P1
3660
8158
578
85.4
globlastp

8

LAB726_H17
silene|11v1|GH293451
3661
8159
578
85.4
globlastp

9

LAB726_H18
strawberry|11v1|CO379965
3662
8160
578
85.4
globlastp

0

LAB726_H18
tripterygium|11v1|SRR09867
3663
8161
578
85.4
globlastp

1
7X130177

LAB726_H40
prunus_mume|13v1|BF71713
3664
8162
578
85.1
globlastp

0
0_P1

LAB726_H18
cacao|10v1|CA797754_P1
3665
8163
578
85.1
globlastp

2

LAB726_H18
cucumber|09v1|CK085635_P
3666
8164
578
85.1
globlastp

3
1

LAB726_H18
dandelion|10v1|DY830349_P
3667
8165
578
85.1
globlastp

4
1

LAB726_H18
euphorbia|11v1|SRR098678X
3668
8166
578
85.1
globlastp

5
10570_P1

LAB726_H18
euphorbia|11v1|SRR098678X
3669
8167
578
85.1
globlastp

6
116144_P1

LAB726_H18
flaveria|11v1|SRR149232.247
3670
8168
578
85.1
globlastp

7
44_P1

LAB726_H18
prunus|10v1|BF717130
3671
8162
578
85.1
globlastp

8

LAB726_H18
watermelon|11v1|AY365247
3672
8169
578
85.1
globlastp

9

LAB726_H19
beech|11v1|SRR006293.1687
3673
8170
578
84.72
glotblastn

0
2_T1

LAB726_H40
zostera|12v1|AM767854_P1
3674
8171
578
84.7
globlastp

1

LAB726_H19
beech|11v1|AM062955XX2_—
3675
8172
578
84.7
globlastp

1
P1

LAB726_H19
clementine|11v1|BQ623057_—
3676
8173
578
84.7
globlastp

2
P1

LAB726_H19
eucalyptus|11v2|CD669443_P
3677
8174
578
84.7
globlastp

3
1

LAB726_H19
kiwi|gb166|FG397185_P1
3678
8175
578
84.7
globlastp

4

LAB726_H19
orange|11v1|BQ623057_P1
3679
8176
578
84.7
globlastp

5

LAB726_H19
phalaenopsis|11v1|SRR12577
3680
8177
578
84.7
globlastp

6
1.1537798_P1

LAB726_H19
poplar|10v1|AI162733
3681
8178
578
84.7
globlastp

7

LAB726_H19
poplar|13v1|AI162232_P1
3682
8178
578
84.7
globlastp

7

LAB726_H19
silene|11v1|GH292050
3683
8179
578
84.7
globlastp

8

LAB726_H19
soybean|11v1|GLYMA05G01
3684
8180
578
84.7
globlastp

9
360

LAB726_H19
soybean|12v1|GLYMA05G01
3685
8180
578
84.7
globlastp

9
360_P1

LAB726_H20
zostera|10v1|AM767854
3686
8171
578
84.7
globlastp

0

LAB726_H40
nicotiana_benthamiana|12v1|
3687
8181
578
84.5
globlastp

2
FS398095_P1

LAB726_H20
poppy|11v1|SRR030259.1517
3688
8182
578
84.5
globlastp

1
4_P1

LAB726_H40
nicotiana_benthamiana|12v1|
3689
8183
578
84.4
globlastp

3
EH616463_P1

LAB726_H20
amborella|12v2|CK760197
3690
8184
578
84.4
globlastp

2

LAB726_H20
amborella|12v3|CK760197_P
3691
8184
578
84.4
globlastp

2
1

LAB726_H20
aquilegia|10v1|DR916092
3692
8185
578
84.4
globlastp

3

LAB726_H20
beet|12v1|BQ585853_P1
3693
8186
578
84.4
globlastp

4

LAB726_H20
cucurbita|11v1|SRR091276X
3694
8187
578
84.4
globlastp

5
100558_P1

LAB726_H20
ginger|gb164|DY345315_P1
3695
8188
578
84.4
globlastp

6

LAB726_H20
grape|11v1|GSVIVT0102007
3696
8189
578
84.4
globlastp

7
1001_P1

LAB726_H20
pseudoroegneria|gb167|FF353
3697
8190
578
84.4
globlastp

8
788

LAB726_H20
rice|11v1|BE040344
3698
8191
578
84.4
globlastp

9

LAB726_H21
trigonella|11v1|SRR066197X
3699
8192
578
84.4
globlastp

0
134539

LAB726_H21
vinca|11v1|SRR098690X1383
3700
8193
578
84.4
globlastp

1
94

LAB726_H21
beech|11v1|SRR364508.2109
3701
8194
578
84.38
glotblastn

2
1_T1

LAB726_H21
oil_palm|11v1|SRR190698.10
3702
8195
578
84.2
globlastp

3
5728_P1

LNU449_H18
apple|11v1|CN492377_T1
3703
8196
578
84.14
glotblastn

LAB726_H40
aquilegia|10v2|DR916092_P1
3704
8197
578
84.1
globlastp

4

LAB726_H21
rye|12v1|DRR001012.157522
3705
8198
578
84.1
globlastp

4

LAB726_H21
rye|12v1|DRR001012.255806
3706
8198
578
84.1
globlastp

5

LAB726_H21
beech|11v1|SRR364434.1069
3707
8199
578
84.03
glotblastn

6
34_T1

LAB726_H40
banana|12v1|FF557627_P1
3708
8200
578
84
globlastp

5

LAB726_H21
b_juncea|10v2|E6ANDIZ01A
3709
8201
578
84
globlastp

7
Z57D

LAB726_H21
clementine|11v1|CF828859_P
3710
8202
578
84
globlastp

8
1

LAB726_H21
fagopyrum|11v1|SRR063703
3711
8203
578
84
globlastp

9
X101349_P1

LAB726_H22
orange|11v1|CF828859_P1
3712
8202
578
84
globlastp

0

LAB726_H40
banana|12v1|BBS2437T3_T1
3713
8204
578
83.78
glotblastn

6

LAB726_H40
blueberry|12v1|SRR353283X
3714
8205
578
83.7
globlastp

7
47351D1_P1

LAB726_H40
onion|12v1|CF443554_P1
3715
8206
578
83.7
globlastp

8

LAB726_H22
apple|11v1|CN489572_P1
3716
8207
578
83.7
globlastp

1

LAB726_H22
avocado|10v1|CO997738_P1
3717
8208
578
83.7
globlastp

2

LAB726_H22
fagopyrum|11v1|SRR063689
3718
8209
578
83.7
globlastp

3
X113457_P1

LAB726_H22
lettuce|10v1|DW088506
3719
8210
578
83.7
globlastp

4

LAB726_H22
lotus|09v1|BW600147_P1
3720
8211
578
83.7
globlastp

5

LAB726_H22
onion|gb162|CF443554
3721
8206
578
83.7
globlastp

6

LAB726_H22
papaya|gb165|EX231164_P1
3722
8212
578
83.7
globlastp

7

LAB726_H22
phalaenopsis|11v1|SRR12577
3723
8213
578
83.7
globlastp

8
1.101540_P1

LAB726_H22
phyla|11v2|SRR099037X102
3724
8214
578
83.7
globlastp

9
964_P1

LAB726_H23
rose|12v1|BI978292
3725
8215
578
83.7
globlastp

0

LAB726_H23
sorghum|12v1|SB07G019760
3726
8216
578
83.7
globlastp

1

LAB726_H23
flaveria|11v1|SRR149241.369
3727
8217
578
83.68
glotblastn

2
236_T1

LAB726_H23
antirrhinum|gb166|AJ795647
3728
8218
578
83.3
globlastp

3
_P1

LAB726_H23
beech|11v1|SRR006293.1183
3729
8219
578
83.3
globlastp

4
1_P1

LAB726_H23
chelidonium|11v1|SRR08475
3730
8220
578
83.3
globlastp

5
2X105402_P1

LAB726_H23
curcuma|10v1|DY387908_P1
3731
8221
578
83.3
globlastp

6

LAB726_H23
eschscholzia|11v1|CD478579
3732
8222
578
83.3
globlastp

7
_P1

LAB726_H23
eucalyptus|11v2|CB967735_P
3733
8223
578
83.3
globlastp

8
1

LAB726_H23
lotus|09v1|CRPLJ030056_P1
3734
8224
578
83.3
globlastp

9

LAB726_H24
maize|10v1|AI372347_P1
3735
8225
578
83.3
globlastp

0

LAB726_H24
soybean|11v1|GLYMA13G05
3736
8226
578
83.3
globlastp

1
120

LAB726_H24
soybean|12v1|GLYMA13G05
3737
8226
578
83.3
globlastp

1
120T3_P1

LAB726_H24
sugarcane|10v1|BQ536230
3738
8227
578
83.3
globlastp

2

LAB726_H24
triphysaria|10v1|EX995758
3739
8228
578
83.3
globlastp

3

LAB726_H24
cowpea|12v1|AM748429_P1
3740
8229
578
83.3
globlastp

4

LAB726_H40
blueberry|12v1|CV189924_P1
3741
8230
578
83
globlastp

9

LAB726_H41
switchgrass|12v1|FL772727_—
3742
8231
578
83
globlastp

0
P1

LAB726_H24
cowpea|gb166|AM748429
3743
8232
578
83
globlastp

4

LAB726_H24
fagopyrum|11v1|SRR063689
3744
8233
578
83
globlastp

5
X14225_P1

LAB726_H24
peanut|10v1|EE124298_P1
3745
8234
578
83
globlastp

6

LAB726_H24
plantago|11v2|SRR066373X1
3746
8235
578
83
globlastp

7
15177_P1

LAB726_H24
fagopyrum|11v1|SRR063703
3747
8236
578
82.99
glotblastn

8
X105112_T1

LAB726_H24
fagopyrum|11v1|SRR063689
3748
8237
578
82.64
glotblastn

9
X114976_T1

LAB726_H41
bean|12v2|CB541531_P1
3749
8238
578
82.6
globlastp

1

LAB726_H41
nicotiana_benthamiana|12v1|
3750
8239
578
82.6
globlastp

2
AJ608729_P1

LAB726_H41
switchgrass|12v1|FE603314_—
3751
8240
578
82.6
globlastp

3
P1

LAB726_H25
bean|12v1|CB541531
3752
8238
578
82.6
globlastp

0

LAB726_H25
chestnut|gb170|FK868407_P1
3753
8241
578
82.6
globlastp

1

LAB726_H25
cotton|11v1|CD485760_P1
3754
8242
578
82.6
globlastp

2

LAB726_H25
eucalyptus|11v2|DRR000893
3755
8243
578
82.6
globlastp

3
X1127112_P1

LAB726_H25
foxtail_millet|11v3|PHY7SI0
3756
8244
578
82.6
globlastp

4
14209M_P1

LAB726_H25
gossypium_raimondii|12v1|D
3757
8242
578
82.6
globlastp

5
T461613_P1

LAB726_H25
ipomoea_nil|10v1|CJ739745_—
3758
8245
578
82.6
globlastp

6
P1

LAB726_H25
medicago|12v1|AW683995_P
3759
8246
578
82.6
globlastp

7
1

LAB726_H25
millet|10v1|CD724348_P1
3760
8247
578
82.6
globlastp

8

LAB726_H25
pigeonpea|11v1|SRR054580X
3761
8248
578
82.6
globlastp

9
109882_P1

LAB726_H26
rice|11v1|CA759391
3762
8249
578
82.6
globlastp

0

LAB726_H26
soybean|11v1|GLYMA19G02
3763
8250
578
82.6
globlastp

1
370

LAB726_H26
soybean|12v1|GLYMA19G02
3764
8250
578
82.6
globlastp

1
370_P1

LAB726_H26
switchgrass|gb167|FE603314
3765
8240
578
82.6
globlastp

2

LAB726_H26
tobacco|gb162|EB425808
3766
8251
578
82.6
globlastp

3

LAB726_H26
trigonella|11v1|SRR066194X
3767
8246
578
82.6
globlastp

4
123567

LAB726_H26
walnuts|gb166|CB303515
3768
8252
578
82.6
globlastp

5

LAB726_H26
b_rapa|11v1|CD816120_P1
3769
8253
578
82.3
globlastp

6

LAB726_H26
canola|11v1|CN732109_P1
3770
8253
578
82.3
globlastp

7

LAB726_H26
canola|11v1|DY006893_P1
3771
8253
578
82.3
globlastp

8

LAB726_H26
canola|11v1|SRR019556.3558
3772
8253
578
82.3
globlastp

9
3_P1

LAB726_H27
eucalyptus|11v2|SRR001659
3773
8254
578
82.3
globlastp

0
X118178_P1

LAB726_H27
oak|10v1|CU656743_P1
3774
8255
578
82.3
globlastp

1

LAB726_H27
oak|10v1|FN724764_P1
3775
8255
578
82.3
globlastp

2

LAB726_H27
oak|10v1|FP038640_P1
3776
8256
578
82.3
globlastp

3

LAB726_H27
platanus|11v1|SRR096786X1
3777
8257
578
82.3
globlastp

4
00632_P1

LAB726_H27
poppy|11v1|FE964336_P1
3778
8258
578
82.3
globlastp

5

LAB726_H27
primula|11v1|SRR098679X11
3779
8259
578
82.3
globlastp

6
0745_P1

LAB726_H27
thellungiella_parvulum|11v1|
3780
8260
578
82.3
globlastp

7
DN774558

LAB726_H27
triphysaria|10v1|EY131978
3781
8261
578
82.3
globlastp

8

LAB726_H27
vinca|11v1|SRR098690X1055
3782
8262
578
82.3
globlastp

9
50

LAB726_H28
humulus|11v1|GD252421_T1
3783
8263
578
82.29
glotblastn

0

LAB726_H28
olea|11v1|SRR014463.24300
3784
8264
578
82.29
glotblastn

1

LAB726_H28
sarracenia|11v1|SRR192669.1
3785
8265
578
82.29
glotblastn

2
22477

LAB726_H28
lotus|09v1|AW719394_P1
3786
8266
578
82
globlastp

3

LAB726_H41
switchgrass|12v1|FL881186_—
3787
8267
578
81.94
glotblastn

4
T1

LAB726_H28
ipomoea_batatas|10v1|EE879
3788
8268
578
81.94
glotblastn

4
156_T1

LAB726_H28
radish|gb164|EW738149
3789
8269
578
81.94
glotblastn

5

LAB726_H28
sarracenia|11v1|SRR192669.1
3790
8270
578
81.94
glotblastn

6
34548

LAB726_H28
artemisia|10v1|EY080949_P1
3791
8271
578
81.9
globlastp

7

LAB726_H28
b_oleracea|gb161|DY027613_—
3792
8272
578
81.9
globlastp

8
P1

LAB726_H28
brachypodium|12v1|BRADI3
3793
8273
578
81.9
globlastp

9
G35370_P1

LAB726_H29
chickpea|11v1|AJ131049
3794
8274
578
81.9
globlastp

0

LAB726_H29
chickpea|13v2|AJ131049_P1
3795
8274
578
81.9
globlastp

0

LAB726_H29
cirsium|11v1|SRR346952.101
3796
8275
578
81.9
globlastp

1
5181_P1

LAB726_H29
fraxinus|11v1|SRR058827.10
3797
8276
578
81.9
globlastp

2
2459_P1

LAB726_H29
nasturtium|11v1|GH163445_P
3798
8277
578
81.9
globlastp

3
1

LAB726_H29
potato|10v1|BG590845_P1
3799
8278
578
81.9
globlastp

4

LAB726_H29
primula|11v1|SRR098679X22
3800
8279
578
81.9
globlastp

5
1763_P1

LAB726_H29
solanum_phureja|09v1|SPHB
3801
8278
578
81.9
globlastp

6
G590845

LAB726_H29
thellungiella_halophilum|11v
3802
8280
578
81.9
globlastp

7
1|DN774558

LAB726_H29
valeriana|11v1|SRR099039X1
3803
8281
578
81.9
globlastp

8
03719

LAB726_H29
cephalotaxus|11v1|SRR06439
3804
8282
578
81.7
globlastp

9
5X100524_P1

LAB726_H30
gossypium_raimondii|12v1|S
3805
8283
578
81.66
glotblastn

0
RR032881.204042_T1

LAB726_H41
switchgrass|12v1|FE635964_—
3806
8284
578
81.6
globlastp

5
P1

LAB726_H41
switchgrass|12v1|GD018323_—
3807
8284
578
81.6
globlastp

6
P1

LAB726_H30
amorphophallus|11v2|SRR08
3808
8285
578
81.6
glotblastn

1
9351X300449_T1

LAB726_H30
cassava|09v1|JGICASSAVA4
3809
8286
578
81.6
glotblastn

2
3762VALIDM1_T1

LAB726_H30
foxtail_millet|11v3|PHY7SI0
3810
8287
578
81.6
glotblastn

3
36858M_T1

LAB726_H30
monkeyflower|12v1|GR15395
3811
8288
578
81.6
globlastp

4
8_P1

LAB726_H30
peanut|10v1|ES711659_P1
3812
8289
578
81.6
globlastp

5

LAB726_H30
radish|gb164|EW732570
3813
8290
578
81.6
globlastp

6

LAB726_H30
scabiosa|11v1|SRR063723X1
3814
8291
578
81.6
globlastp

7
01769

LAB726_H30
soybean|11v1|GLYMA02G02
3815
8292
578
81.6
globlastp

8
540

LAB726_H30
soybean|12v1|GLYMA02G02
3816
8292
578
81.6
globlastp

8
530_P1

LAB726_H30
switchgrass|gb167|FE635964
3817
8284
578
81.6
globlastp

9

LAB726_H31
fagopyrum|11v1|SRR063703
3818
8293
578
81.31
glotblastn

0
X106005_T1

LAB726_H31
sequoia|10v1|SRR065044S00
3819
8294
578
81.3
globlastp

1
06791

LAB726_H31
rye|12v1|DRR001012.205262
3820
8295
578
81.25
glotblastn

2

LAB726_H31
sarracenia|11v1|SRR192669.1
3821
8296
578
81.25
glotblastn

3
2655

LAB726_H31
wheat|10v2|BE405232
3822
8295
578
81.25
glotblastn

4

LAB726_H31
wheat|12v3|BE405232_T1
3823
8295
578
81.25
glotblastn

4

LAB726_H34
switchgrass|12v1|FL995735_—
3824
8297
578
81.25
glotblastn

1
T1

LAB726_H31
sarracenia|11v1|SRR192669.1
3825
8298
578
81.23
glotblastn

5
04561

LAB726_H41
wheat|12v3|BG274974_P1
3826
8299
578
81.2
globlastp

7

LAB726_H41
wheat|12v3|CA606376_P1
3827
8299
578
81.2
globlastp

8

LAB726_H41
wheat|12v3|CA656686_P1
3828
8299
578
81.2
globlastp

9

LAB726_H42
wheat|12v3|CA670179_P1
3829
8299
578
81.2
globlastp

0

LAB726_H42
zostera|12v1|SRR057351X11
3830
8300
578
81.2
globlastp

1
838D1_P1

LAB726_H31
arabidopsis|10v1|AT1G69840
3831
8301
578
81.2
globlastp

6
_P1

LAB726_H31
brachypodium|12v1|BRADI4
3832
8302
578
81.2
globlastp

7
G28640_P1

LAB726_H31
cannabis|12v1|GR221241_P1
3833
8303
578
81.2
globlastp

8

LAB726_H31
cenchrus|gb166|AF325721_P
3834
8304
578
81.2
globlastp

9
1

LAB726_H32
leymus|gb166|EG384892_P1
3835
8305
578
81.2
globlastp

0

LAB726_H32
petunia|gb171|CV293421_P1
3836
8306
578
81.2
globlastp

1

LAB726_H32
radish|gb164|EV525651
3837
8307
578
81.2
globlastp

2

LAB726_H32
sorghum|12v1|SB02G022890
3838
8308
578
81.2
globlastp

3

LAB726_H32
wheat|10v2|BE416273
3839
8299
578
81.2
globlastp

4

LAB726_H32
wheat|12v3|BE416273_P1
3840
8299
578
81.2
globlastp

4

LAB726_H32
zostera|10v1|SRR057351S001
3841
8300
578
81.2
globlastp

5
1839

LAB726_H32
centaurea|gb166|EL934388_T
3842
8309
578
81.08
glotblastn

6
1

LAB726_H32
distylium|11v1|SRR065077X
3843
8310
578
81
globlastp

7
100271_P1

LAB726_H42
wheat|12v3|BE417232_P1
3844
8311
578
80.9
globlastp

2

LAB726_H32
arabidopsis_lyrata|09v1|JGIA
3845
8312
578
80.9
globlastp

8
L007199_P1

LAB726_H32
barley|10v2|AK248988
3846
8313
578
80.9
glotblastn

9

LAB726_H32
barley|12v1|AK248988_T1
3847
8313
578
80.9
glotblastn

9

LAB726_H33
fraxinus|11v1|SRR058827.10
3848
8314
578
80.9
glotblastn

0
8481_T1

LAB726_H33
millet|10v1|EVO454PM0158
3849
8315
578
80.9
globlastp

1
46_P1

LAB726_H33
oak|10v1|FN732286_P1
3850
8316
578
80.9
globlastp

2

LAB726_H33
oat|11v1|CN814781_P1
3851
8317
578
80.9
globlastp

3

LAB726_H33
pseudoroegneria|gb167|FF340
3852
8318
578
80.9
globlastp

4
799

LAB726_H33
rye|12v1|BE587309
3853
8318
578
80.9
globlastp

5

LAB726_H33
rye|12v1|DRR001012.100122
3854
8318
578
80.9
globlastp

6

LAB726_H33
rye|12v1|DRR001012.102416
3855
8318
578
80.9
globlastp

7

LAB726_H33
rye|12v1|DRR001012.197214
3856
8318
578
80.9
globlastp

8

LAB726_H33
rye|12v1|DRR001015.137689
3857
8319
578
80.9
globlastp

9

LAB726_H34
spruce|11v1|ES853572
3858
8320
578
80.9
globlastp

0

LAB726_H34
switchgrass|gb167|FL881186
3859
8321
578
80.9
glotblastn

1

LAB726_H34
maritime_pine|10v1|BX68055
3860
8322
578
80.69
glotblastn

2
9_T1

LAB726_H42
olea|13v1|SRR014463X41682
3861
8323
578
80.6
globlastp

3
D1_P1

LAB726_H42
prunus_mume|13v1|DRR0019
3862
8324
578
80.6
globlastp

4
05.20720_P1

LAB726_H34
b_rapa|11v1|CX192388_P1
3863
8325
578
80.6
globlastp

3

LAB726_H34
barley|10v2|BE413353
3864
8326
578
80.6
globlastp

4

LAB726_H34
barley|12v1|BE413353_P1
3865
8326
578
80.6
globlastp

4

LAB726_H34
cirsium|11v1|SRR346952.101
3866
8327
578
80.6
globlastp

5
596_P1

LAB726_H34
foxtail_millet|11v3|PHY7SI0
3867
8328
578
80.6
globlastp

6
30729M_P1

LAB726_H34
oat|11v1|GO589928_P1
3868
8329
578
80.6
globlastp

7

LAB726_H34
peanut|10v1|EG028649_P1
3869
8330
578
80.6
globlastp

8

LAB726_H34
prunus|10v1|PPA020997M
3870
8331
578
80.6
globlastp

9

LAB726_H35
rye|12v1|DRR001012.205576
3871
8332
578
80.6
globlastp

0

LAB726_H35
pseudoroegneria|gb167|FF345
3872
8333
578
80.56
glotblastn

1
177

LAB726_H35
rice|11v1|GFXAC027038X15
3873
8334
578
80.56
glotblastn

2

LAB726_H35
spruce|11v1|ES868640
3874
8335
578
80.56
glotblastn

3

LAB726_H35
cannabis|12v1|JK501803_P1
3875
8336
578
80.4
globlastp

4

LAB726_H42
cowpea|12v1|FF383069_T1
3876
8337
578
80.28
glotblastn

5

LAB726_H35
podocarpus|10v1|SRR065014
3877
8338
578
80.28
glotblastn

5
S0000335_T1

LAB726_H35
barley|10v2|CA027627
3878
8339
578
80.21
glotblastn

6

LAB726_H35
barley|12v1|BE413100_T1
3879
8339
578
80.21
glotblastn

6

LAB726_H35
cirsium|11v1|SRR346952.125
3880
8340
578
80.21
glotblastn

7
051_T1

LAB726_H35
cleome_spinosa|10v1|GR9336
3881
8341
578
80.21
glotblastn

8
11_T1

LAB726_H35
gerbera|09v1|AJ754218_T1
3882
8342
578
80.21
glotblastn

9

LAB726_H36
maize|10v1|AF236374_T1
3883
8343
578
80.21
glotblastn

0

LAB726_H36
oil_palm|11v1|GH636081_T1
3884
8344
578
80.21
glotblastn

1

LAB726_H36
rye|12v1|DRR001012.140984
3885
8345
578
80.21
glotblastn

2

LAB726_H36
rye|12v1|DRR001012.312907
3886
8346
578
80.21
glotblastn

3

LAB726_H42
bean|12v2|SRR001334.30029
3887
8347
578
80.2
globlastp

6
5_P1

LAB726_H36
amsonia|11v1|SRR098688X1
3888
8348
578
80.2
globlastp

4
11084_P1

LAB726_H36
canola|11v1|Ev171301_P1
3889
8349
578
80.2
globlastp

5

LAB726_H36
fescue|gb161|DT689570_P1
3890
8350
578
80.2
globlastp

6

LAB726_H36
pigeonpea|11v1|SRR054580X
3891
8351
578
80.2
globlastp

7
134167_P1

LAB726_H36
pseudotsuga|10v1|SRR06511
3892
8352
578
80.2
globlastp

8
9S0005689

LAB726_H36
rye|12v1|BE493928
3893
8353
578
80.2
globlastp

9

LAB726_H37
rye|12v1|DRR001012.163121
3894
8353
578
80.2
globlastp

0

LAB726_H37
abies|11v2|SRR098676X1214
3895
8354
578
80
glotblastn

1
16_T1

LAB727_H1
foxtail_millet|11v3|PHY7SI0
3896
8355
579
87.8
globlastp

01678M_P1

LAB727_H2
maize|10v1|BG319933_P1
3897
8356
579
86.1
globlastp

LAB727_H3
wheat|12v3|AL823037_T1
3898
8357
579
84.47
glotblastn

LAB728_H1
brachypodium|12v1|BRADI3
3899
8358
580
93.4
globlastp

G45600_P1

LAB728_H2
foxtail_millet|11v3|PHY7SI0
3900
8359
580
92.4
globlastp

16733M_P1

LAB728_H3
sorghum|12v1|SB04G022500
3901
8360
580
92
globlastp

LAB728_H14
switchgrass|12v1|FL904190_—
3902
8361
580
90.8
globlastp

0
P1

LAB728_H4
maize|10v1|AW126454_P1
3903
8362
580
90.7
globlastp

LAB728_H14
switchgrass|12v1|FL762967_—
3904
8363
580
88.4
globlastp

1
P1

LAB728_H14
switchgrass|12v1|FL690101_—
3905
8364
580
85.6
globlastp

2
P1

LAB728_H5
foxtail_millet|11v3|PHY7SI0
3906
8365
580
85.6
globlastp

26154M_P1

LAB728_H6
sorghum|12v1|SB05G005240
3907
8366
580
85.6
globlastp

LAB728_H7
switchgrass|gb167|FE606045
3908
8367
580
85.4
globlastp

LAB728_H14
banana|12v1|BBS3230T3_P1
3909
8368
580
85.3
globlastp

3

LAB728_H14
banana|12v1|MAGEN201203
3910
8369
580
85.3
globlastp

4
1954_P1

LAB728_H8
oil_palm|11v1|EL685212_P1
3911
8370
580
85.3
globlastp

LAB728_H9
rice|11v1|AA231730
3912
8371
580
85.3
globlastp

LAB728_H14
banana|12v1|MAGEN201200
3913
8372
580
84.7
globlastp

5
6094_P1

LAB728_H10
cotton|11v1|BG443576_P1
3914
8373
580
84.7
globlastp

LAB728_H11
maize|10v1|AW355852_P1
3915
8374
580
84.7
globlastp

LAB728_H12
oil_palm|11v1|GH637480_P1
3916
8375
580
84.7
globlastp

LAB728_H13
brachypodium|12v1|BRADI4
3917
8376
580
84.6
globlastp

G23990_P1

LAB728_H14
grape|11v1|GSVIVT0102334
3918
8377
580
84.1
globlastp

3001_P1

LAB728_H15
prunus|10v1|BU048263
3919
8378
580
84
globlastp

LAB728_H14
prunus_mume|13v1|BU04826
3920
8379
580
83.7
globlastp

6
3_P1

LAB728_H16
strawberry|11v1|DV438068
3921
8380
580
83.7
globlastp

LAB728_H17
wheat|10v2|BE400578
3922
8381
580
83.7
globlastp

LAB728_H14
barley|12v1|AV916799_P1
3923
8382
580
83.6
globlastp

7

LAB728_H14
switchgrass|12v1|FE608007_—
3924
8383
580
83.6
globlastp

8
P1

LAB728_H18
aristolochia|10v1|SRR039082
3925
8384
580
83.6
globlastp

S0017898_P1

LAB728_H19
solanum_phureja|09v1|SPHA
3926
8385
580
83.6
globlastp

W218930

LAB728_H20
rye|12v1|DRR001012.117287
3927
8386
580
83.58
glotblastn

LAB728_H21
rye|12v1|DRR001012.101144
3928
8387
580
83.4
globlastp

LAB728_H22
orange|11v1|CF505289_T1
3929
8388
580
83.31
glotblastn

LAB728_H14
aquilegia|10v2|DR916618_P1
3930
8389
580
83.3
globlastp

9

LAB728_H23
aquilegia|10v1|DR916618
3931
8389
580
83.3
globlastp

LAB728_H24
clementine|11v1|CF505289_P
3932
8390
580
83.3
globlastp

1

LAB728_H25
eucalyptus|11v2|CD669654_P
3933
8391
580
83.3
globlastp

1

LAB728_H26
sorghum|12v1|SB08G004730
3934
8392
580
83.2
globlastp

LAB728_H27
sugarcane|10v1|BQ533242
3935
8393
580
83.2
globlastp

LAB728_H28
tomato|11v1|AW218930
3936
8394
580
83.2
globlastp

LAB728_H35
wheat|12v3|BE401438_P1
3937
8395
580
83.2
globlastp

LAB728_H29
foxtail_millet|11v3|PHY7SI0
3938
8396
580
83.1
globlastp

09738M_P1

LAB728_H30
rice|11v1|AU091909
3939
8397
580
83.1
globlastp

LAB728_H31
apple|11v1|CN488913_P1
3940
8398
580
83
globlastp

LAB728_H32
maize|10v1|AI600968_P1
3941
8399
580
83
globlastp

LAB728_H33
maize|10v1|AI612399_P1
3942
8400
580
83
globlastp

LAB728_H34
monkeyflower|12v1|DV20841
3943
8401
580
83
globlastp

9_P1

LAB728_H35
wheat|10v2|BE400911
3944
8402
580
83
globlastp

LAB728_H36
potato|10v1|BG095839_P1
3945
8403
580
82.9
globlastp

LAB728_H37
rye|12v1|BE588002
3946
8404
580
82.9
globlastp

LAB728_H38
solanum_phureja|09v1|SPHB
3947
8403
580
82.9
globlastp

G130883

LAB728_H15
nicotiana_benthamiana|12v1|
3948
8405
580
82.8
globlastp

0
BP746865_P1

LAB728_H39
cotton|11v1|BQ401499_P1
3949
8406
580
82.8
globlastp

LAB728_H40
cotton|11v1|BQ411048XX1_—
3950
8407
580
82.8
glotblastn

T1

LAB728_H15
wheat|12v3|BE499310_P1
3951
8408
580
82.7
globlastp

1

LAB728_H15
wheat|12v3|BG263848_P1
3952
8409
580
82.7
globlastp

2

LAB728_H41
apple|11v1|CN444346_P1
3953
8410
580
82.7
globlastp

LAB728_H42
tomato|11v1|BG130883
3954
8411
580
82.6
globlastp

LAB728_H15
banana|12v1|ES432280_P1
3955
8412
580
82.5
globlastp

3

LAB728_H15
wheat|12v3|BE499606_P1
3956
8413
580
82.5
globlastp

4

LAB728_H43
brachypodium|12v1|BRADI4
3957
8414
580
82.5
globlastp

G41270_P1

LAB728_H44
cotton|11v1|AI725838_P1
3958
8415
580
82.5
globlastp

LAB728_H68
poplar|13v1|BU834302_P1
3959
8416
580
82.5
globlastp

LAB728_H15
zostera|12v1|SRR057351X10
3960
8417
580
82.4
globlastp

5
0431D1_P1

LAB728_H15
soybean|12v1|GLYMA08G20
3961
8418
580
82.3
globlastp

6
100_P1

LAB728_H45
chestnut|gb170|SRR006295S0
3962
8419
580
82.3
globlastp

007143_P1

LAB728_H46
euonymus|11v1|SRR070038X
3963
8420
580
82.3
globlastp

161748_P1

LAB728_H47
pigeonpea|11v1|GR466200_P
3964
8421
580
82.3
globlastp

1

LAB728_H48
ambrosia|11v1|SRR346935.1
3965
8422
580
82.27
glotblastn

0455_T1

LAB728_H15
bean|12v2|SRR001334.10811
3966
8423
580
82.24
glotblastn

7
0_T1

LAB728_H50
barley|10v2|BG299366
3967
8424
580
82.2
globlastp

LAB728_H51
humulus|11v1|EX516617XX1
3968
8425
580
82.2
globlastp

_P1

LAB728_H52
oak|10v1|CU656922_P1
3969
8426
580
82.2
globlastp

LAB728_H53
chelidonium|11v1|SRR08475
3970
8427
580
82.12
glotblastn

2X101408_T1

LAB728_H54
euonymus|11v1|SRR070038X
3971
8428
580
82.1
globlastp

167447_P1

LAB728_H55
poppy|11v1|FE964330XX1_P
3972
8429
580
82.1
globlastp

1

LAB728_H56
poppy|11v1|SRR030259.1061
3973
8430
580
82.1
globlastp

14_P1

LAB728_H57
soybean|11v1|GLYMA12G29
3974
8431
580
82.1
globlastp

120

LAB728_H57
soybean|12v1|GLYMA12G29
3975
8431
580
82.1
globlastp

120_P1

LAB728_H15
switchgrass|12v1|FL704878_—
3976
8432
580
82
globlastp

8
P1

LAB728_H58
watermelon|11v1|CO995207
3977
8433
580
82
globlastp

LAB728_H59
eschscholzia|11v1|SRR01411
3978
8434
580
81.96
glotblastn

6.117210_T1

LAB728_H60
cassava|09v1|DR085771_P1
3979
8435
580
81.9
globlastp

LAB728_H61
valeriana|11v1|SRR099039X1
3980
8436
580
81.9
globlastp

02448

LAB728_H62
amorphophallus|11v2|SRR08
3981
8437
580
81.8
glotblastn

9351X204773_T1

LAB728_H63
gossypium_raimondii|12v1|B
3982
8438
580
81.8
globlastp

Q401499_P1

LAB728_H64
sunflower|12v1|AJ828364
3983
8439
580
81.8
globlastp

LAB728_H65
cassava|09v1|CK645768_T1
3984
8440
580
81.79
glotblastn

LAB728_H15
switchgrass|12v1|DN152589_—
3985
8441
580
81.7
globlastp

9
P1

LAB728_H66
amsonia|11v1|SRR098688X1
3986
8442
580
81.7
globlastp

01148_P1

LAB728_H67
flaveria|11v1|SRR149229.121
3987
8443
580
81.7
globlastp

129_P1

LAB728_H68
poplar|10v1|BU834302
3988
8444
580
81.7
globlastp

LAB728_H69
melon|10v1|AM725980_P1
3989
8445
580
81.6
globlastp

LAB728_H70
flaveria|11v1|SRR149229.248
3990
8446
580
81.59
glotblastn

624_T1

LAB728_H71
ambrosia|11v1|SRR346943.1
3991
8447
580
81.51
glotblastn

79042_T1

LAB728_H16
wheat|12v3|BE400578_P1
3992
8448
580
81.5
globlastp

0

LAB728_H50
barley|12v1|BG299366_P1
3993
8449
580
81.5
globlastp

LAB728_H72
arabidopsis_lyrata|09v1|JGIA
3994
8450
580
81.5
globlastp

L001111_P1

LAB728_H73
arabidopsis|10v1|AT1G10950
3995
8451
580
81.5
globlastp

_P1

LAB728_H74
cucumber|09v1|CO995207_P
3996
8452
580
81.5
globlastp

1

LAB728_H75
tripterygium|11v1|SRR09867
3997
8453
580
81.45
glotblastn

7X101310

LAB728_H76
castorbean|12v1|EG668282_P
3998
8454
580
81.4
globlastp

1

LAB728_H77
phalaenopsis|11v1|SRR12577
3999
8455
580
81.4
globlastp

1.102425_P1

LAB728_H16
olea|13v1|SRR014463X15625
4000
8456
580
81.3
globlastp

1
D1_P1

LAB728_H78
distylium|11v1|SRR065077X
4001
8457
580
81.3
glotblastn

102421_T1

LAB728_H79
gossypium_raimondii|12v1|AI
4002
8458
580
81.3
globlastp

725838_P1

LAB728_H80
sequoia|10v1|SRR065044S00
4003
8459
580
81.3
globlastp

08008

LAB728_H81
soybean|11v1|GLYMA08G20
4004
8460
580
81.3
globlastp

100

LAB728_H82
sunflower|12v1|EE634605
4005
8461
580
81.3
globlastp

LAB728_H83
thellungiella_halophilum|11v
4006
8462
580
81.3
globlastp

1|DN776428

LAB728_H97
poplar|13v1|AI161458_P1
4007
8463
580
81.3
globlastp

LAB728_H84
canola|11v1|EE438738XX1_—
4008
8464
580
81.28
glotblastn

T1

LAB728_H85
amborella|12v3|SRR038634.2
4009
8465
580
81.2
globlastp

3347_P1

LAB728_H86
phalaenopsis|11v1|SRR12577
4010
8466
580
81.2
globlastp

1.1045236_P1

LAB728_H87
b_rapa|11v1|DY029522_T1
4011
8467
580
81.11
glotblastn

LAB728_H88
canola|11v1|EE482164_T1
4012
8468
580
81.11
glotblastn

LAB728_H16
chickpea|13v2|SRR133517.12
4013
8469
580
81.1
globlastp

2
4960_P1

LAB728_H89
flaveria|11v1|SRR149232.235
4014
8470
580
81.1
globlastp

751_P1

LAB728_H90
plantago|11v2|SRR066373X1
4015
8471
580
81.1
globlastp

23829_P1

LAB728_H91
tomato|11v1|AW980045
4016
8472
580
81.1
globlastp

LAB728_H16
zostera|12v1|SRR057351X10
4017
8473
580
81.08
glotblastn

3
4986D1_T1

LAB728_H92
ambrosia|11v1|SRR346935.1
4018
8474
580
81.08
glotblastn

73589_T1

LAB728_H93
zostera|10v1|SRR057351S000
4019
8475
580
81.08
glotblastn

3050

LAB728_H94
abies|11v2|SRR098676X1083
4020
8476
580
81
globlastp

00_P1

LAB728_H95
flaveria|11v1|SRR149229.177
4021
8477
580
81
glotblastn

296_T1

LAB728_H96
phalaenopsis|11v1|CB035062
4022
8478
580
81
globlastp

_P1

LAB728_H97
poplar|10v1|BI129900
4023
8479
580
81
globlastp

LAB728_H98
soybean|11v1|GLYMA05G30
4024
8480
580
81
globlastp

210

LAB728_H98
soybean|12v1|GLYMA05G30
4025
8480
580
81
globlastp

210_P1

LAB728_H99
thellungiella_parvulum|11v1|
4026
8481
580
80.94
glotblastn

BY802665

LAB728_H10
vinca|11v1|SRR098690X2070
4027
8482
580
80.91
glotblastn

0
17

LAB728_H10
arnica|11v1|SRR099034X103
4028
8483
580
80.9
globlastp

1
714_P1

LAB728_H10
artemisia|10v1|SRR019254S0
4029
8484
580
80.9
globlastp

2
005312_P1

LAB728_H10
canola|11v1|EE536558_P1
4030
8485
580
80.9
globlastp

3

LAB728_H10
thellungiella_halophilum|11v
4031
8486
580
80.9
globlastp

4
1|EC599452

LAB728_H16
bean|12v2|CB540471_P1
4032
8487
580
80.8
globlastp

4

LAB728_H16
zostera|12v1|SRR057351X10
4033
8488
580
80.8
globlastp

5
8977D1_P1

LAB728_H10
b_rapa|11v1|CD814617_P1
4034
8489
580
80.8
globlastp

5

LAB728_H10
bean|12v1|CB540471
4035
8487
580
80.8
globlastp

6

LAB728_H10
canola|11v1|EE482091_P1
4036
8490
580
80.8
globlastp

7

LAB728_H10
grape|11v1|GSVIVT0102605
4037
8491
580
80.8
globlastp

8
7001_P1

LAB728_H10
medicago|12v1|AA660311_P
4038
8492
580
80.8
globlastp

9
1

LAB728_H11
pigeonpea|11v1|GW355237_—
4039
8493
580
80.8
globlastp

0
P1

LAB728_H11
solanum_phureja|09v1|SPHA
4040
8494
580
80.8
globlastp

1
W980045

LAB728_H11
soybean|11v1|GLYMA08G13
4041
8495
580
80.8
globlastp

2
370

LAB728_H11
soybean|12v1|GLYMA08G13
4042
8495
580
80.8
globlastp

2
370_P1

LAB728_H11
tabernaemontana|11v1|SRR09
4043
8496
580
80.8
globlastp

3
8689X121083

LAB728_H11
sciadopitys|10v1|SRR065035
4044
8497
580
80.74
glotblastn

4
S0101685

LAB728_H11
cedrus|11v1|SRR065007X115
4045
8498
580
80.7
globlastp

5
59_P1

LAB728_H11
orobanche|10v1|SRR023189S
4046
8499
580
80.7
globlastp

6
0001474_P1

LAB728_H11
peanut|10v1|EG029513_P1
4047
8500
580
80.7
globlastp

7

LAB728_H11
ambrosia|11v1|SRR346935.1
4048
8501
580
80.64
glotblastn

8
08182_T1

LAB728_H11
cannabis|12v1|GR221358_T1
4049
8502
580
80.64
glotblastn

9

LAB728_H12
zostera|10v1|SRR057351S000
4050
8503
580
80.61
glotblastn

0
7836

LAB728_H12
pine|10v2|AW290741_P1
4051
8504
580
80.6
globlastp

1

LAB728_H12
pseudotsuga|10v1|SRR06511
4052
8505
580
80.6
globlastp

2
9S0022990

LAB728_H12
trigonella|11v1|SRR066194X
4053
8506
580
80.6
globlastp

3
128074

LAB728_H16
amborella|12v3|SRR038644.5
4054
8507
580
80.57
glotblastn

6
08290_T1

LAB728_H12
ambrosia|11v1|SRR346935.2
4055
8508
580
80.5
glotblastn

4
22870_T1

LAB728_H12
euphorbia|11v1|DV119884_P
4056
8509
580
80.5
globlastp

5
1

LAB728_H12
thellungiella_parvulum|11v1|
4057
8510
580
80.5
globlastp

6
DN776428

LAB728_H12
triphysaria|10v1|EY173064
4058
8511
580
80.41
glotblastn

7

LAB728_H12
orobanche|10v1|SRR023189S
4059
8512
580
80.4
globlastp

8
0004653_P1

LAB728_H12
ambrosia|11v1|SRR346935.1
4060
8513
580
80.3
globlastp

9
78351_P1

LAB728_H13
trigonella|11v1|SRR066194X
4061
8514
580
80.3
globlastp

0
115429

LAB728_H13
lotus|09v1|BP041471_T1
4062
8515
580
80.27
glotblastn

1

LAB728_H13
centaurea|gb166|EH713087_T
4063
8516
580
80.24
glotblastn

2
1

LAB728_H16
blueberry|12v1|Cv191186_T
4064
8517
580
80.23
glotblastn

7
1

LAB728_H16
cowpea|12v1|FF395857_P1
4065
8518
580
80.2
globlastp

8

LAB728_H13
monkeyflower|12v1|DV20854
4066
8519
580
80.2
globlastp

3
5_P1

LAB728_H13
ambrosia|11v1|SRR346935.3
4067
8520
580
80.13
glotblastn

4
34999_T1

LAB728_H13
amorphophallus|11v2|SRR08
4068
8521
580
80.1
globlastp

5
9351X102383_P1

LAB728_H13
b_rapa|11v1|CD828399_P1
4069
8522
580
80.1
globlastp

6

LAB728_H13
canola|11v1|EE562156_T1
4070
8523
580
80.1
glotblastn

7

LAB728_H13
cirsium|11v1|SRR346952.102
4071
8524
580
80.1
globlastp

8
5144_P1

LAB728_H13
medicago|12v1|AI974779_P1
4072
8525
580
80.1
globlastp

9

LAB729_H1
barley|10v2|AV834870
4073
8526
581
80.59
glotblastn

LAB729_H2
foxtail_millet|11v3|PHY7SI0
4074
8527
581
80.59
glotblastn

06747M_T1

LAB729_H5
switchgrass|12v1|FE635085_—
4075
8528
581
80.59
glotblastn

T1

LAB729_H3
maize|10v1|BI233587_P1
4076
8529
581
80.2
globlastp

LAB729_H4
lovegrass|gb167|EH188628_T
4077
8530
581
80.17
glotblastn

1

LAB729_H5
switchgrass|gb167|FE635086
4078
8531
581
80.17
glotblastn

LAB733_H1
rice|11v1|BE228642
4079
8532
585
97.5
globlastp

LAB733_H2
brachypodium|12v1|BRADI2
4080
8533
585
84.1
globlastp

G15630_P1

LAB733_H3
foxtail_millet|11v3|EC613863
4081
8534
585
81.4
globlastp

_P1

LAB733_H4
oat|11v1|GR316970_P1
4082
8535
585
81
globlastp

LAB733_H7
switchgrass|12v1|FE644413_—
4083
8536
585
80.7
globlastp

P1

LAB733_H8
switchgrass|12v1|DN143122_—
4084
8537
585
80.4
globlastp

P1

LAB733_H9
wheat|12v3|BE591325_T1
4085
8538
585
80
glotblastn

LAB733_H5
barley|10v2|BE437797
4086
8539
585
80
globlastp

LAB733_H6
maize|10v1|BM501431_T1
4087
8540
585
80
glotblastn

LAB734_H1
sorghum|12v1|SB04G030260
4088
8541
586
80.7
globlastp

LAB735_H1
foxtail_millet|11v3|PHY7SI0
4089
8542
587
86.8
globlastp

29502M_P1

LAB735_H2
sorghum|12v1|SB02G038090
4090
8543
587
86.1
globlastp

LAB735_H6
barley|12v1|AW982945_P1
4091
8544
587
85.3
globlastp

LAB735_H3
maize|10v1|T70636_P1
4092
8545
587
85
globlastp

LAB735_H4
brachypodium|12v1|BRADI1
4093
8546
587
82.9
globlastp

G22870_P1

LAB735_H5
maize|10v1|CD568493_P1
4094
8547
587
82.3
globlastp

LAB736_H1
sugarcane|10v1|AA644740
4095
8548
588
94.2
globlastp

LAB736_H2
foxtail_millet|11v3|EC613905
4096
8549
588
93.82
glotblastn

_T1

LAB736_H3
foxtail_millet|11v3|EC612809
4097
8550
588
93.8
globlastp

_P1

LAB736_H4
millet|10v1|CD725278_P1
4098
8551
588
93.8
globlastp

LAB736_H5
switchgrass|gb167|DW17724
4099
8552
588
93.8
globlastp

3

LAB736_H6
switchgrass|gb167|FE642331
4100
8552
588
93.8
globlastp

LAB736_H5, L
switchgrass|12v1|FE598015_—
4101
8552
588
93.8
globlastp

AB736_H6
P1

LAB736_H7
maize|10v1|AA030697_P1
4102
8553
588
93.4
globlastp

LAB736_H8
millet|10v1|EVO454PM0101
4103
8554
588
93.4
globlastp

91_P1

LAB736_H9
rice|11v1|AA750586
4104
8555
588
93.4
globlastp

LAB736_H10
rice|11v1|AA751627
4105
8555
588
93.4
globlastp

LAB736_H11
sugarcane|10v1|CA068735
4106
8556
588
93.4
globlastp

LAB736_H12
switchgrass|gb167|DN143437
4107
8557
588
93.4
globlastp

LAB736_H13
switchgrass|12v1|FE598001_—
4108
8558
588
93.4
globlastp

P1

LAB736_H13
switchgrass|gb167|FE598001
4109
8558
588
93.4
globlastp

LAB736_H15
barley|10v2|BE412864
4110
8559
588
93.1
globlastp

LAB736_H16
cenchrus|gb166|BM084845_P
4111
8560
588
93.1
globlastp

1

LAB736_H17
cenchrus|gb166|EB653522_P
4112
8561
588
93.1
globlastp

1

LAB736_H18
foxtail_millet|11v3|PHY7SI0
4113
8562
588
93.1
globlastp

07119M_P1

LAB736_H19
leymus|gb166|CN465854_P1
4114
8559
588
93.1
globlastp

LAB736_H22
sorghum|12v1|SB02G029380
4115
8563
588
93.1
globlastp

LAB736_H23
sorghum|12v1|SB02G029400
4116
8563
588
93.1
globlastp

LAB736_H12
switchgrass|12v1|DN143437_—
4117
8564
588
93.05
glotblastn

T1

LAB736_H94
wheat|12v3|BE398874_P1
4118
8565
588
92.7
globlastp

LAB736_H15
barley|12v1|BE412864_P1
4119
8565
588
92.7
globlastp

LAB736_H20
rye|12v1|DRR001012.109618
4120
8565
588
92.7
globlastp

LAB736_H24
maize|10v1|AI665475_P1
4121
8566
588
92.7
globlastp

LAB736_H25
maize|10v1|AI491408_P1
4122
8567
588
92.3
globlastp

LAB736_H14
rye|12v1|DRR001013.119239
4123
8568
588
92.28
glotblastn

LAB736_H21
rye|12v1|DRR001012.137575
4124
8569
588
91.9
globlastp

LAB736_H27
fescue|gb161|DT676666_P1
4125
8570
588
91.9
globlastp

LAB736_H28
leymus|gb166|CN466276_P1
4126
8571
588
91.9
globlastp

LAB736_H31
pseudoroegneria|gb167|FF340
4127
8571
588
91.9
globlastp

278

LAB736_H33
wheat|10v2|BE398359
4128
8572
588
91.9
globlastp

LAB736_H26
barley|10v2|BE413337
4129
8573
588
91.5
globlastp

LAB736_H26
barley|12v1|BE413337_P1
4130
8573
588
91.5
globlastp

LAB736_H29
oat|11v1|GO583356_P1
4131
8574
588
91.5
globlastp

LAB736_H32
rye|12v1|BE588084
4132
8573
588
91.5
globlastp

LAB736_H33
wheat|12v3|BE398359_P1
4133
8573
588
91.5
globlastp

LAB736_H34
oat|11v1|GO587864_P1
4134
8575
588
91.5
globlastp

LAB736_H95
banana|12v1|ES432602_P1
4135
8576
588
91.1
globlastp

LAB736_H96
banana|12v1|FF560412_P1
4136
8577
588
91.1
globlastp

LAB736_H97
banana|12v1|MAGEN201202
4137
8576
588
91.1
globlastp

9387_P1

LAB736_H30
oil_palm|11v1|EL595260XX2
4138
8578
588
90.7
globlastp

_P1

LAB736_H38
brachypodium|12v1|BRADI4
4139
8579
588
90.7
globlastp

G34750_P1

LAB736_H39
brachypodium|12v1|BRADI5
4140
8579
588
90.7
globlastp

G24680_P1

LAB736_H98
banana|12v1|FF559351_P1
4141
8580
588
90.3
globlastp

LAB736_H35
rye|12v1|BE586513
4142
8581
588
90.3
globlastp

LAB736_H41
amorphophallus|11v2|SRR08
4143
8582
588
90.3
globlastp

9351X108750-P1

LAB736_H36
oil_palm|11v1|EL685376_P1
4144
8583
588
90
globlastp

LAB736_H37
rye|12v1|BE495288
4145
8584
588
90
globlastp

LAB736_H44
platanus|11v1|SRR096786X1
4146
8585
588
90
globlastp

01987_P1

LAB736_H45
amborella|12v2|CK763796
4147
8586
588
90
globlastp

LAB736_H99
banana|12v1|FL650674_P1
4148
8587
588
89.6
globlastp

LAB736_H40
oil_palm|11v1|EL693924_P1
4149
8588
588
89.6
globlastp

LAB736_H49
phalaenopsis|11v1|CB032502
4150
8589
588
89.6
globlastp

_P1

LAB736_H43
banana|10v1|FF559351
4151
8590
588
89.58
glotblastn

LAB736_H47
liriodendron|gb166|CK74700
4152
8591
588
89.2
globlastp

1_P1

LAB736_H42
amorphophallus|11v2|SRR08
4153
8592
588
89.19
glotblastn

9351X100023_T1

LAB736_H10
banana|12v1|FF560304_P1
4154
8593
588
88.5
globlastp

0

LAB736_H55
chestnut|gb170|SRR006295S0
4155
8594
588
88.5
globlastp

000018_P1

LAB736_H57
oak|10v1|DN950219_P1
4156
8594
588
88.5
globlastp

LAB736_H58
oak|10v1|FN710662_P1
4157
8594
588
88.5
globlastp

LAB736_H52
avocado|10v1|CV005126_P1
4158
8595
588
88.4
globlastp

LAB736_H61
nuphar|gb166|CK743266_P1
4159
8596
588
88.4
globlastp

LAB736_H46
clementine|11v1|CF834680_P
4160
8597
588
88.1
globlastp

1

LAB736_H48
orange|11v1|CF834680_P1
4161
8597
588
88.1
globlastp

LAB736_H10
prunus_mume|13v1|SRR3456
4162
8598
588
88
globlastp

1
74.29976_P1

LAB736_H10
zostera|12v1|AM771243_P1
4163
8599
588
88
globlastp

2

LAB736_H51
rye|12v1|DRR001012.132032
4164
8600
588
88
globlastp

LAB736_H63
phalaenopsis|11v1|SRR12577
4165
8601
588
88
globlastp

1.1002084_P1

LAB736_H69
eschscholzia|11v1|CK746882
4166
8602
588
88
globlastp

_P1

LAB736_H70
eschscholzia|11v1|SRR01411
4167
8603
588
88
globlastp

6.107174_P1

LAB736_H74
zostera|10v1|AM771243
4168
8599
588
88
globlastp

LAB736_H10
amborella|12v3|CK763796_P
4169
8604
588
87.9
globlastp

3
1

LAB736_H54
rye|12v1|BE496010
4170
8605
588
87.9
globlastp

LAB736_H56
chestnut|gb170|SRR006295S0
4171
8606
588
87.7
globlastp

000020_P1

LAB736_H93
thalictrum|11v1|SRR096787X
4172
8607
588
87.64
glotblastn

118621

LAB736_H53
peanut|10v1|ES703917_P1
4173
8608
588
87.6
globlastp

LAB736_H59
cotton|11v1|CO092918_P1
4174
8609
588
87.6
globlastp

LAB736_H60
gossypium_raimondii|12v1|D
4175
8609
588
87.6
globlastp

R453902_P1

LAB736_H66
chelidonium|11v1|SRR08475
4176
8610
588
87.6
globlastp

2X105613_P1

LAB736_H87
platanus|11v1|SRR096786X1
4177
8611
588
87.6
globlastp

00009_P1

LAB736_H88
poppy|11v1|SRR030259.1186
4178
8612
588
87.6
globlastp

12_P1

LAB736_H10
aristolochia|10v1|SRR039082
4179
8613
588
87.3
globlastp

4
S0167593_P1

LAB736_H10
eschscholzia|11v1|SRR01411
4180
8614
588
87.3
globlastp

5
6.101239_P1

LAB736_H62
peanut|10v1|EE124632_P1
4181
8615
588
87.3
globlastp

LAB736_H65
prunus|10v1|PPA018912M
4182
8616
588
87.3
globlastp

LAB736_H67
cotton|11v1|AI054819_P1
4183
8617
588
87.3
globlastp

LAB736_H68
cotton|11v1|BQ402123XX1_—
4184
8618
588
87.3
globlastp

P1

LAB736_H71
gossypium_raimondii|12v1|B
4185
8619
588
87.3
globlastp

Q407784_P1

LAB736_H77
aristolochia|10v1|FD760421_—
4186
8620
588
87.3
globlastp

P1

LAB736_H86
nasturtium|11v1|SRR032558.
4187
8621
588
87.3
globlastp

104348_P1

LAB736_H91
spruce|11v1|ES878796
4188
8622
588
87.3
globlastp

LAB736_H10
amborella|12v3|FD431280_T
4189
8623
588
87.26
glotblastn

6
1

LAB736_H10
cacao|10v1|CA796703_P1
4190
8624
588
86.9
globlastp

7

LAB736_H10
chelidonium|11v1|SRR08475
4191
8625
588
86.9
globlastp

8
2X112713_P1

LAB736_H10
chickpea|13v2|GR396963_P1
4192
8626
588
86.9
globlastp

9

LAB736_H11
eschscholzia|11v1|SRR01411
4193
8627
588
86.9
globlastp

0
6.101817_P1

LAB736_H11
grape|11v1|GSVIVT0103305
4194
8628
588
86.9
globlastp

1
0001_P1

LAB736_H11
hornbeam|12v1|SRR364455.1
4195
8629
588
86.9
globlastp

2
06623_P1

LAB736_H11
nasturtium|11v1|SRR032558.
4196
8630
588
86.9
globlastp

3
105454_P1

LAB736_H11
poppy|11v1|SRR030259.1453
4197
8631
588
86.9
globlastp

4
18_P1

LAB736_H11
zostera|12v1|SRR057351X10
4198
8632
588
86.9
globlastp

5
7705D1_P1

LAB736_H72
pigeonpea|11v1|EE604706_P
4199
8633
588
86.9
globlastp

1

LAB736_H73
soybean|11v1|GLYMA11G11
4200
8634
588
86.9
globlastp

040

LAB736_H73
soybean|12v1|GLYMA11G11
4201
8634
588
86.9
globlastp

040_P1

LAB736_H78
cacao|10v1|CU471632_P1
4202
8635
588
86.9
globlastp

LAB736_H81
cotton|11v1|BE055064XX1_P
4203
8636
588
86.9
globlastp

1

LAB736_H83
gossypium_raimondii|12v1|AI
4204
8636
588
86.9
globlastp

054819_P1

LAB736_H84
gossypium_raimondii|12v1|B
4205
8637
588
86.9
globlastp

E051919_P1

LAB736_H89
rose|12v1|BI978658
4206
8638
588
86.9
globlastp

LAB736_H92
strawberry|11v1|DV440629
4207
8639
588
86.9
globlastp

LAB736_H75
apple|11v1|CO052839_T1
4208
8640
588
86.87
glotblastn

LAB736_H11
abies|11v2|SRR098676X1069
4209
8641
588
86.5
globlastp

6
54_P1

LAB736_H11
blueberry|12v1|CF810829_P1
4210
8642
588
86.5
globlastp

7

LAB736_H11
cedrus|11v1|SRR065007X112
4211
8643
588
86.5
globlastp

8
685_P1

LAB736_H11
eschscholzia|11v1|SRR01411
4212
8644
588
86.5
globlastp

9
6.12977_P1

LAB736_H12
eucalyptus|11v2|DR410025_P
4213
8645
588
86.5
globlastp

0
1

LAB736_H12
euonymus|11v1|SRR070038X
4214
8646
588
86.5
globlastp

1
10220_P1

LAB736_H12
grape|11v1|GSVIVT0100858
4215
8647
588
86.5
globlastp

2
5001_P1

LAB736_H12
hornbeam|12v1|SRR364455.1
4216
8648
588
86.5
globlastp

3
09238_P1

LAB736_H12
ipomoea_nil|10v1|BJ553366_—
4217
8649
588
86.5
globlastp

4
P1

LAB736_H12
kiwi|gb166|FG400583_P1
4218
8650
588
86.5
globlastp

5

LAB736_H12
nasturtium|11v1|SRR032558.
4219
8651
588
86.5
globlastp

6
113300_P1

LAB736_H12
pigeonpea|11v1|SRR054580X
4220
8652
588
86.5
globlastp

7
122588_P1

LAB736_H12
poppy|11v1|FE966899_P1
4221
8653
588
86.5
globlastp

8

LAB736_H12
poppy|11v1|SRR030259.6629
4222
8654
588
86.5
globlastp

9
6_P1

LAB736_H13
poppy|11v1|SRR030263.2680
4223
8655
588
86.5
globlastp

0
44_P1

LAB736_H13
rose|12v1|EC589036_P1
4224
8656
588
86.5
globlastp

1

LAB736_H79
cassava|09v1|CK644576_P1
4225
8657
588
86.5
globlastp

LAB736_H80
cotton|11v1|BE051919_P1
4226
8658
588
86.5
globlastp

LAB736_H82
cotton|11v1|CO074939_P1
4227
8659
588
86.5
globlastp

LAB736_H90
soybean|11v1|GLYMA12G03
4228
8660
588
86.5
globlastp

230

LAB736_H90
soybean|12v1|GLYMA12G03
4229
8660
588
86.5
globlastp

230_P1

LAB736_H13
blueberry|12v1|Cv191532_T
4230
8661
588
86.49
glotblastn

2
1

LAB736_H13
pigeonpea|11v1|SRR054580X
4231
8662
588
86.49
glotblastn

3
120749_T1

LAB736_H13
spruce|11v1|SRR065813X172
4232
8663
588
86.49
glotblastn

4
800XX2_T1

LAB736_H13
clover|gb162|BB902472_P1
4233
8664
588
86.2
globlastp

5

LAB736_H13
soybean|12v1|GLYMA02G00
4234
8665
588
86.2
globlastp

6
540T2_P1

LAB736_H13
clementine|11v1|EY756332_P
4235
8666
588
86.1
globlastp

7
1

LAB736_H13
distylium|11v1|SRR065077X
4236
8667
588
86.1
globlastp

8
101515_P1

LAB736_H13
euonymus|11v1|SRR070038X
4237
8668
588
86.1
globlastp

9
105593_P1

LAB736_H14
ginger|gb164|DY366887_P1
4238
8669
588
86.1
globlastp

0

LAB736_H14
ipomoea_nil|10v1|BJ554172_—
4239
8670
588
86.1
globlastp

1
P1

LAB736_H14
kiwi|gb166|FG403536_P1
4240
8671
588
86.1
globlastp

2

LAB736_H14
maritime_pine|10v1|BX24993
4241
8672
588
86.1
globlastp

3
7_P1

LAB736_H14
nasturtium|11v1|GH167097_—
4242
8673
588
86.1
glotblastn

4
T1

LAB736_H14
orange|11v1|SRR099042X12
4243
8666
588
86.1
globlastp

5
0439_P1

LAB736_H14
pine|10v2|AA556512_P1
4244
8674
588
86.1
globlastp

6

LAB736_H14
pine|10v2|AA739809_P1
4245
8675
588
86.1
globlastp

7

LAB736_H14
poppy|11v1|SRR030263.3019
4246
8676
588
86.1
globlastp

8
41_P1

LAB736_H14
primula|11v1|SRR098679X11
4247
8677
588
86.1
globlastp

9
5137_P1

LAB736_H15
prunus_mume|13v1|BU03950
4248
8678
588
86.1
globlastp

0
4_P1

LAB736_H15
prunus|10v1|BU039504_P1
4249
8678
588
86.1
globlastp

1

LAB736_H15
sciadopitys|10v1|SRR065035
4250
8679
588
86.1
globlastp

2
S0002289_P1

LAB736_H15
sequoia|10v1|SRR065044S00
4251
8680
588
86.1
globlastp

3
04259_P1

LAB736_H15
sequoia|10v1|SRR065044S00
4252
8681
588
86.1
globlastp

4
13081_P1

LAB736_H15
spruce|11v1|CO226532XX2_—
4253
8682
588
86.1
glotblastn

5
T1

LAB736_H15
spruce|11v1|ES245525_P1
4254
8683
588
86.1
globlastp

6

LAB736_H15
spruce|11v1|ES246400_P1
4255
8683
588
86.1
globlastp

7

LAB736_H15
spruce|11v1|EX346680_P1
4256
8683
588
86.1
globlastp

8

LAB736_H15
spruce|11v1|EX357435_P1
4257
8683
588
86.1
globlastp

9

LAB736_H16
spruce|11v1|GW735401_T1
4258
8684
588
86.1
glotblastn

0

LAB736_H16
walnuts|gb166|EL890883_P1
4259
8685
588
86.1
globlastp

1

LAB736_H64
avocado|10v1|CK748322_P1
4260
8686
588
86.1
globlastp

LAB736_H85
hevea|10v1|EC601153_P1
4261
8687
588
86.1
globlastp

LAB736_H16
beech|11v1|SRR006294.1279
4262
8688
588
85.8
globlastp

2
2_P1

LAB736_H16
soybean|12v1|GLYMA10G00
4263
8689
588
85.8
globlastp

3
890_P1

LAB736_H16
trigonella|11v1|SRR066194X
4264
8690
588
85.8
globlastp

4
107494_P1

LAB736_H16
chelidonium|11v1|SRR08475
4265
8691
588
85.71
glotblastn

5
2X103293_T1

LAB736_H16
flaveria|11v1|SRR149229.103
4266
8692
588
85.71
glotblastn

6
791_T1

LAB736_H16
poppy|11v1|SRR030259.1024
4267
8693
588
85.71
glotblastn

7
52_T1

LAB736_H16
spruce|11v1|EX347396XX1_—
4268
8694
588
85.71
glotblastn

8
T1

LAB736_H16
bean|12v2|CA898006_P1
4269
8695
588
85.7
globlastp

9

LAB736_H17
beech|11v1|SRR006293.1351
4270
8696
588
85.7
globlastp

0
_P1

LAB736_H17
cassava|09v1|CK649579_P1
4271
8697
588
85.7
globlastp

1

LAB736_H17
cassava|09v1|CK652178_P1
4272
8698
588
85.7
globlastp

2

LAB736_H17
cassava|09v1|DB921837_P1
4273
8699
588
85.7
globlastp

3

LAB736_H17
catharanthus|11v1|EG556652
4274
8700
588
85.7
globlastp

4
_P1

LAB736_H17
cucumber|09v1|GD179495_P
4275
8701
588
85.7
globlastp

5
1

LAB736_H17
flaveria|11v1|SRR149229.120
4276
8702
588
85.7
globlastp

6
920_P1

LAB736_H17
flaveria|11v1|SRR149229.151
4277
8703
588
85.7
globlastp

7
152_P1

LAB736_H17
flaveria|11v1|SRR149232.107
4278
8703
588
85.7
globlastp

8
239_P1

LAB736_H17
flaveria|11v1|SRR149232.110
4279
8704
588
85.7
globlastp

9
412XX2_P1

LAB736_H18
flaveria|11v1|SRR149238.161
4280
8703
588
85.7
globlastp

0
140_P1

LAB736_H18
ipomoea_batatas|10v1|BM878
4281
8705
588
85.7
globlastp

1
730_P1

LAB736_H18
kiwi|gb166|FG396383_P1
4282
8706
588
85.7
globlastp

2

LAB736_H18
lettuce|12v1|DW047012_P1
4283
8707
588
85.7
globlastp

3

LAB736_H18
poppy|11v1|SRR030259.1582
4284
8708
588
85.7
globlastp

4
49_P1

LAB736_H18
primula|11v1|SRR098679X11
4285
8709
588
85.7
globlastp

5
5190_P1

LAB736_H18
pseudotsuga|10v1|SRR06511
4286
8710
588
85.7
globlastp

6
9S0016570_P1

LAB736_H18
valeriana|11v1|SRR099039X1
4287
8711
588
85.7
globlastp

7
05607_P1

LAB736_H18
coffea|10v1|DV663644_P1
4288
8712
588
85.4
globlastp

8

LAB736_H18
poppy|11v1|SRR030259.1044
4289
8713
588
85.33
glotblastn

9
91_T1

LAB736_H19
amsonia|11v1|SRR098688X1
4290
8714
588
85.3
globlastp

0
01879XX1_P1

LAB736_H19
aquilegia|10v2|DT730656_P1
4291
8715
588
85.3
globlastp

1

LAB736_H19
basilicum|10v1|DY328816_P
4292
8716
588
85.3
globlastp

2
1

LAB736_H19
bean|12v2|CA898001_P1
4293
8717
588
85.3
globlastp

3

LAB736_H19
cucurbita|11v1|SRR091276X
4294
8718
588
85.3
globlastp

4
116595_P1

LAB736_H19
eucalyptus|11v2|CT982618_P
4295
8719
588
85.3
globlastp

5
1

LAB736_H19
flaveria|11v1|SRR149229.106
4296
8720
588
85.3
globlastp

6
615XX1_P1

LAB736_H19
flaveria|11v1|SRR149229.213
4297
8721
588
85.3
globlastp

7
778_P1

LAB736_H19
flaveria|11v1|SRR149232.124
4298
8722
588
85.3
globlastp

8
299_P1

LAB736_H19
flaveria|11v1|SRR149244.108
4299
8723
588
85.3
globlastp

9
574_P1

LAB736_H20
lettuce|12v1|DW046271_P1
4300
8724
588
85.3
globlastp

0

LAB736_H20
podocarpus|10v1|SRR065014
4301
8725
588
85.3
globlastp

1
S0033625_P1

LAB736_H20
poplar|13v1|AI161831_P1
4302
8726
588
85.3
globlastp

2

LAB736_H20
poplar|13v1|BU830427_P1
4303
8727
588
85.3
globlastp

3

LAB736_H20
sunflower|12v1|CD854677_P
4304
8728
588
85.3
globlastp

4
1

LAB736_H20
sunflower|12v1|CF076744_P1
4305
8728
588
85.3
globlastp

5

LAB736_H20
sunflower|12v1|DY915995_P
4306
8729
588
85.3
globlastp

6
1

LAB736_H20
sunflower|12v1|DY916466_P
4307
8728
588
85.3
globlastp

7
1

LAB736_H20
sunflower|12v1|DY933489_P
4308
8728
588
85.3
globlastp

8
1

LAB736_H20
sunflower|12v1|EE632782_P1
4309
8728
588
85.3
globlastp

9

LAB736_H21
tabernaemontana|11v1|SRR09
4310
8730
588
85.3
globlastp

0
8689X100198_P1

LAB736_H21
tragopogon|10v1|SRR020205
4311
8731
588
85.3
globlastp

1
S0000111_P1

LAB736_H21
tragopogon|10v1|SRR020205
4312
8732
588
85.3
globlastp

2
S0001935_P1

LAB736_H21
tripterygium|11v1|SRR09867
4313
8733
588
85.3
globlastp

3
7X10006_P1

LAB736_H21
tripterygium|11v1|SRR09867
4314
8733
588
85.3
globlastp

4
7X106720_P1

LAB736_H21
castorbean|12v1|CF981381_T
4315
8734
588
84.94
glotblastn

5
1

LAB736_H21
catharanthus|11v1|AM232370
4316
8735
588
84.94
glotblastn

6
_T1

LAB736_H21
phyla|11v2|SRR099037X150
4317
8736
588
84.94
glotblastn

7
513_T1

LAB736_H21
sarracenia|11v1|SRR192669.1
4318
8737
588
84.94
glotblastn

8
07869_T1

LAB736_H21
sorghum|12v1|SB07G026082
4319
8738
588
84.94
glotblastn

9
_T1

LAB736_H22
tragopogon|10v1|SRR020205
4320
8739
588
84.94
glotblastn

0
S0037787_T1

LAB736_H22
ambrosia|11v1|SRR346943.4
4321
8740
588
84.9
globlastp

1
0528_P1

LAB736_H22
amsonia|11v1|SRR098688X1
4322
8741
588
84.9
globlastp

2
02951_P1

LAB736_H22
arnica|11v1|SRR099034X100
4323
8742
588
84.9
globlastp

3
590_P1

LAB736_H22
blueberry|12v1|SRR353282X
4324
8743
588
84.9
globlastp

4
23303D1_P1

LAB736_H22
castorbean|12v1|EG660381_P
4325
8744
588
84.9
globlastp

5
1

LAB736_H22
cephalotaxus|11v1|SRR06439
4326
8745
588
84.9
globlastp

6
5X105184_P1

LAB736_H22
cichorium|gb171|EH672726_—
4327
8746
588
84.9
globlastp

7
P1

LAB736_H22
cotton|11v1|DR459437_P1
4328
8747
588
84.9
globlastp

8

LAB736_H22
cowpea|12v1|FG813262_P1
4329
8748
588
84.9
globlastp

9

LAB736_H23
cucurbita|11v1|SRR091276X
4330
8749
588
84.9
globlastp

0
103724_P1

LAB736_H23
cucurbita|11v1|SRR091276X
4331
8750
588
84.9
globlastp

1
105006_P1

LAB736_H23
cynara|gb167|GE585720_P1
4332
8751
588
84.9
globlastp

2

LAB736_H23
euonymus|11v1|SRR070038X
4333
8752
588
84.9
globlastp

3
103272_P1

LAB736_H23
flax|11v1|JG029731_P1
4334
8753
588
84.9
globlastp

4

LAB736_H23
gossypium_raimondii|12v1|D
4335
8747
588
84.9
globlastp

5
R459437_P1

LAB736_H23
kiwi|gb166|FG408675_P1
4336
8754
588
84.9
globlastp

6

LAB736_H23
poplar|13v1|AI162308_P1
4337
8755
588
84.9
globlastp

7

LAB736_H23
poplar|13v1|BI132334_P1
4338
8756
588
84.9
globlastp

8

LAB736_H23
sesame|12v1|JK047611_P1
4339
8757
588
84.9
globlastp

9

LAB736_H24
sunflower|12v1|DY943109_P
4340
8758
588
84.9
globlastp

0
1

LAB736_H24
sunflower|12v1|EL422321_P1
4341
8759
588
84.9
globlastp

1

LAB736_H24
tabernaemontana|11v1|SRR09
4342
8760
588
84.9
globlastp

2
8689X10126_P1

LAB736_H24
tripterygium|11v1|SRR09867
4343
8761
588
84.9
globlastp

3
7X113122_P1

LAB736_H24
valeriana|11v1|SRR099039X1
4344
8762
588
84.9
globlastp

4
08870_P1

LAB736_H24
valeriana|11v1|SRR099039X1
4345
8763
588
84.9
globlastp

5
34053_P1

LAB736_H24
nicotiana_benthamiana|12v1|
4346
8764
588
84.62
glotblastn

6
EB427301_T1

LAB736_H24
ambrosia|11v1|SRR346935.1
4347
8765
588
84.6
globlastp

7
21008_P1

LAB736_H24
ambrosia|11v1|SRR346935.1
4348
8766
588
84.6
globlastp

8
28177_P1

LAB736_H24
castorbean|12v1|EE254608_P
4349
8767
588
84.6
globlastp

9
1

LAB736_H25
cichorium|gb171|EH685572_—
4350
8768
588
84.6
globlastp

0
P1

LAB736_H25
cirsium|11v1|SRR346952.107
4351
8769
588
84.6
globlastp

1
645_P1

LAB736_H25
cirsium|11v1|SRR346952.113
4352
8770
588
84.6
globlastp

2
099_P1

LAB736_H25
cryptomeria|gb166|BW99429
4353
8771
588
84.6
globlastp

3
4_P1

LAB736_H25
cucumber|09v1|AM721621_P
4354
8772
588
84.6
globlastp

4
1

LAB736_H25
cucurbita|11v1|SRR091276X
4355
8773
588
84.6
globlastp

5
134320_P1

LAB736_H25
flax|11v1|GW864708_P1
4356
8774
588
84.6
globlastp

6

LAB736_H25
grape|11v1|GSVIVT0103654
4357
8775
588
84.6
globlastp

7
0001_P1

LAB736_H25
lotus|09v1|AI967802_P1
4358
8776
588
84.6
globlastp

8

LAB736_H25
melon|10v1|AM721621_P1
4359
8777
588
84.6
globlastp

9

LAB736_H26
momordica|10v1|SRR071315
4360
8778
588
84.6
globlastp

0
S0003271_P1

LAB736_H26
phyla|11v2|SRR099035X121
4361
8779
588
84.6
globlastp

1
827_P1

LAB736_H26
phyla|11v2|SRR099035X227
4362
8780
588
84.6
globlastp

2
65_P1

LAB736_H26
podocarpus|10v1|SRR065014
4363
8781
588
84.6
globlastp

3
S0011977_P1

LAB736_H26
poplar|13v1|AI161478_P1
4364
8782
588
84.6
globlastp

4

LAB736_H26
pseudotsuga|10v1|SRR06511
4365
8783
588
84.6
globlastp

5
9S0007565_P1

LAB736_H26
rye|12v1|DRR001012.194009
4366
8784
588
84.6
globlastp

6
_P1

LAB736_H26
thalictrum|11v1|SRR096787X
4367
8785
588
84.6
globlastp

7
113214_P1

LAB736_H26
tobacco|gb162|CV017258_P1
4368
8786
588
84.6
globlastp

8

LAB736_H26
valeriana|11v1|SRR099039X1
4369
8787
588
84.6
globlastp

9
10882_P1

LAB736_H27
vinca|11v1|SRR098690X1149
4370
8788
588
84.6
globlastp

0
77_P1

LAB736_H27
watermelon|11v1|AM721621
4371
8789
588
84.6
globlastp

1
_P1

LAB736_H27
arnica|11v1|SRR099034X115
4372
8790
588
84.56
glotblastn

2
562_T1

LAB736_H27
cirsium|11v1|SRR346952.100
4373
8791
588
84.56
glotblastn

3
2504_T1

LAB736_H27
flaveria|11v1|SRR149232.100
4374
8792
588
84.56
glotblastn

4
37_T1

LAB736_H27
phyla|11v2|SRR099037X120
4375
8793
588
84.56
glotblastn

5
018_T1

LAB736_H27
thalictrum|11v1|SRR096787X
4376
8794
588
84.56
glotblastn

6
102413_T1

LAB736_H27
ambrosia|11v1|SRR346935.2
4377
8795
588
84.2
globlastp

7
00534_P1

LAB736_H27
aquilegia|10v2|JGIAC009830
4378
8796
588
84.2
globlastp

8
_P1

LAB736_H27
arnica|11v1|SRR099034X116
4379
8797
588
84.2
globlastp

9
220XX1_P1

LAB736_H28
catharanthus|11v1|EG560971
4380
8798
588
84.2
globlastp

0
_P1

LAB736_H28
centaurea|gb166|EH714103_P
4381
8799
588
84.2
globlastp

1
1

LAB736_H28
centaurea|gb166|EH717477_P
4382
8800
588
84.2
globlastp

2
1

LAB736_H28
centaurea|gb166|EH719179_P
4383
8799
588
84.2
globlastp

3
1

LAB736_H28
centaurea|gb166|EH777742_P
4384
8800
588
84.2
globlastp

4
1

LAB736_H28
chickpea|13v2|FE669101_P1
4385
8801
588
84.2
globlastp

5

LAB736_H28
cirsium|11v1|SRR346952.105
4386
8799
588
84.2
globlastp

6
6417_P1

LAB736_H28
cirsium|11v1|SRR346952.113
4387
8800
588
84.2
globlastp

7
6935_P1

LAB736_H28
dandelion|10v1|DY820686_P
4388
8802
588
84.2
globlastp

8
1

LAB736_H28
dandelion|10v1|DY823032_P
4389
8803
588
84.2
globlastp

9
1

LAB736_H29
medicago|12v1|AA660765_P
4390
8804
588
84.2
globlastp

0
1

LAB736_H29
monkeyflower|12v1|DV20622
4391
8805
588
84.2
globlastp

1
9_P1

LAB736_H29
nicotiana_benthamiana|12v1|
4392
8806
588
84.2
globlastp

2
AJ718619_P1

LAB736_H29
nicotiana_benthamiana|12v1|
4393
8807
588
84.2
globlastp

3
AY310805_P1

LAB736_H29
nicotiana_benthamiana|12v1|
4394
8808
588
84.2
globlastp

4
BP751876_P1

LAB736_H29
safflower|gb162|EL378862_P
4395
8800
588
84.2
globlastp

5
1

LAB736_H29
safflower|gb162|EL386347_P
4396
8799
588
84.2
globlastp

6
1

LAB736_H29
salvia|10v1|CV166698_P1
4397
8809
588
84.2
globlastp

7

LAB736_H29
spurge|gb161|BI993548_P1
4398
8810
588
84.2
globlastp

8

LAB736_H29
sunflower|12v1|CD851537_P
4399
8811
588
84.2
globlastp

9
1

LAB736_H30
sunflower|12v1|CD851768_P
4400
8812
588
84.2
globlastp

0
1

LAB736_H30
sunflower|12v1|CD851975_P
4401
8813
588
84.2
globlastp

1
1

LAB736_H30
sunflower|12v1|CX946573_P
4402
8814
588
84.2
globlastp

2
1

LAB736_H30
sunflower|12v1|DY916584_P
4403
8811
588
84.2
globlastp

3
1

LAB736_H30
tobacco|gb162|EB446005_P1
4404
8807
588
84.2
globlastp

4

LAB736_H30
vinca|11v1|SRR098690X1041
4405
8815
588
84.2
globlastp

5
83_P1

LAB736_H30
vinca|11v1|SRR098690X1180
4406
8816
588
84.2
globlastp

6
43_P1

LAB736_H30
ambrosia|11v1|SRR346943.1
4407
8817
588
84.17
glotblastn

7
03510_T1

LAB736_H30
ginger|gb164|DY365135_T1
4408
8818
588
84.17
glotblastn

8

LAB736_H30
guizotia|10v1|GE568006_T1
4409
8819
588
84.17
glotblastn

9

LAB736_H31
epimedium|11v1|SRR013502.
4410
8820
588
84
globlastp

0
12372_P1

LAB736_H31
apple|11v1|CN444563_P1
4411
8821
588
83.8
globlastp

1

LAB736_H31
artemisia|10v1|EY040905_P1
4412
8822
588
83.8
globlastp

2

LAB736_H31
bupleurum|11v1|SRR301254.
4413
8823
588
83.8
globlastp

3
109062_P1

LAB736_H31
cephalotaxus|11v1|SRR06439
4414
8824
588
83.8
globlastp

4
5X100755_P1

LAB736_H31
cleome_spinosa|10v1|SRR015
4415
8825
588
83.8
globlastp

5
531S0004626_P1

LAB736_H31
cucumber|09v1|CK085809_P
4416
8826
588
83.8
globlastp

6
1

LAB736_H31
euphorbia|11v1|SRR098678X
4417
8827
588
83.8
globlastp

7
114301_P1

LAB736_H31
fagopyrum|11v1|SRR063689
4418
8828
588
83.8
globlastp

8
X10433_P1

LAB736_H31
flax|11v1|JG030614_P1
4419
8829
588
83.8
globlastp

9

LAB736_H32
fraxinus|11v1|SRR058827.11
4420
8830
588
83.8
globlastp

0
8860_P1

LAB736_H32
nicotiana_benthamiana|12v1|
4421
8831
588
83.8
globlastp

1
BP744671_P1

LAB736_H32
sunflower|12v1|DY946843_P
4422
8832
588
83.8
globlastp

2
1

LAB736_H32
taxus|10v1|SRR032523S0000
4423
8833
588
83.8
globlastp

3
376_P1

LAB736_H32
taxus|10v1|SRR032523S0007
4424
8834
588
83.8
globlastp

4
416_P1

LAB736_H32
watermelon|11v1|AM714282
4425
8835
588
83.8
globlastp

5
_P1

LAB736_H32
ambrosia|11v1|SRR346935.1
4426
8836
588
83.78
glotblastn

6
39842_T1

LAB736_H32
ambrosia|11v1|SRR346943.1
4427
8836
588
83.78
glotblastn

7
00107_T1

LAB736_H32
bruguiera|gb166|BP939977_T
4428
8837
588
83.77
glotblastn

8
1

LAB736_H32
eggplant|10v1|FS002632_P1
4429
8838
588
83.5
globlastp

9

LAB736_H33
fagopyrum|11v1|SRR063689
4430
8839
588
83.46
glotblastn

0
X29084_T1

LAB736_H33
arnica|11v1|SRR099034X102
4431
8840
588
83.4
globlastp

1
482_P1

LAB736_H33
artemisia|10v1|SRR019254S0
4432
8841
588
83.4
globlastp

2
015330_P1

LAB736_H33
cannabis|12v1|JK493102_P1
4433
8842
588
83.4
globlastp

3

LAB736_H33
cirsium|11v1|SRR346952.103
4434
8843
588
83.4
globlastp

4
512_P1

LAB736_H33
euonymus|11v1|SRR070038X
4435
8844
588
83.4
globlastp

5
10672_P1

LAB736_H33
euphorbia|11v1|DV126326_P
4436
8845
588
83.4
globlastp

6
1

LAB736_H33
humulus|11v1|EX517745_P1
4437
8842
588
83.4
globlastp

7

LAB736_H33
melon|10v1|AM714282_P1
4438
8846
588
83.4
globlastp

8

LAB736_H33
orobanche|10v1|SRR023189S
4439
8847
588
83.4
globlastp

9
0012506_P1

LAB736_H34
rye|12v1|DRR001012.121140
4440
8848
588
83.4
glotblastn

0
_T1

LAB736_H34
senecio|gb170|DY663749_P1
4441
8849
588
83.4
globlastp

1

LAB736_H34
trigonella|11v1|SRR066194X
4442
8850
588
83.4
globlastp

2
10492_P1

LAB736_H34
triphysaria|10v1|SRR023500S
4443
8851
588
83.4
globlastp

3
0004223_P1

LAB736_H34
gnetum|10v1|EX946344_P1
4444
8852
588
83.1
globlastp

4

LAB736_H34
ambrosia|11v1|SRR346935.1
4445
8853
588
83.01
glotblastn

5
52382_T1

LAB736_H34
flaveria|11v1|SRR149232.105
4446
8854
588
83.01
glotblastn

6
25_T1

LAB736_H34
lotus|09v1|LLBW594340_T1
4447
8855
588
83.01
glotblastn

7

LAB736_H34
utricularia|11v1|SRR094438.1
4448
8856
588
83.01
glotblastn

8
00830_T1

LAB736_H34
cleome_spinosa|10v1|GR9316
4449
8857
588
83
globlastp

9
81_P1

LAB736_H35
cleome_spinosa|10v1|GR9316
4450
8858
588
83
globlastp

0
94_P1

LAB736_H35
dandelion|10v1|DY809241_P
4451
8859
588
83
globlastp

1
1

LAB736_H35
olea|13v1|SRR014463X11285
4452
8860
588
83
globlastp

2
D1_P1

LAB736_H35
olea|13v1|SRR014463X20456
4453
8861
588
83
globlastp

3
D1_P1

LAB736_H35
olea|13v1|SRR014463X50558
4454
8862
588
83
globlastp

4
D1_P1

LAB736_H35
triphysaria|10v1|BM356527_—
4455
8863
588
83
globlastp

5
P1

LAB736_H35
triphysaria|10v1|EX992837_P
4456
8863
588
83
globlastp

6
1

LAB736_H35
tripterygium|11v1|SRR09867
4457
8864
588
83
globlastp

7
7X107213_P1

LAB736_H35
fagopyrum|11v1|SRR063689
4458
8865
588
82.7
globlastp

8
X102658_P1

LAB736_H35
tomato|11v1|BG124605_P1
4459
8866
588
82.7
globlastp

9

LAB736_H36
fagopyrum|11v1|SRR063703
4460
8867
588
82.69
glotblastn

0
X101240_T1

LAB736_H36
utricularia|11v1|SRR094438.1
4461
8868
588
82.63
glotblastn

1
1242 _T1

LAB736_H36
wheat|12v3|CA484492_T1
4462
8869
588
82.63
glotblastn

2

LAB736_H36
olea|13v1|SRR014464X37685
4463
8870
588
82.6
globlastp

3
D1_P1

LAB736_H36
orobanche|10v1|SRR023189S
4464
8871
588
82.6
globlastp

4
0006337_P1

LAB736_H36
thellungiella_parvulum|11v1|
4465
8872
588
82.6
globlastp

5
BY802561_P1

LAB736_H36
tripterygium|11v1|SRR09867
4466
8873
588
82.6
globlastp

6
7X101979_P1

LAB736_H36
fagopyrum|11v1|GO898729_—
4467
8874
588
82.4
globlastp

7
P1

LAB736_H36
fagopyrum|11v1|SRR063689
4468
8875
588
82.4
globlastp

8
X111618_P1

LAB736_H36
fern|gb171|BP916158_P1
4469
8876
588
82.4
globlastp

9

LAB736_H37
liquorice|gb171|FS241495_P1
4470
8877
588
82.3
globlastp

0

LAB736_H37
nicotiana_benthamiana|12v1|
4471
8878
588
82.3
globlastp

1
BP746782_P1

LAB736_H37
potato|10v1|BF053135_P1
4472
8879
588
82.3
globlastp

2

LAB736_H37
potato|10v1|BF460091_P1
4473
8880
588
82.3
globlastp

3

LAB736_H37
solanum_phureja|09v1|SPHB
4474
8880
588
82.3
globlastp

4
G124605_P1

LAB736_H37
artemisia|10v1|EY045386_T1
4475
8881
588
82.24
glotblastn

5

LAB736_H37
b_rapa|11v1|H74713_P1
4476
8882
588
82.2
globlastp

6

LAB736_H37
canola|11v1|DY028402_P1
4477
8883
588
82.2
globlastp

7

LAB736_H37
cleome_spinosa|10v1|SRR015
4478
8884
588
82.2
globlastp

8
531S0002670_P1

LAB736_H37
humulus|11v1|GD249260_P1
4479
8885
588
82.2
globlastp

9

LAB736_H38
monkeyflower|12v1|DV20648
4480
8886
588
82.2
globlastp

0
8_P1

LAB736_H38
olea|13v1|SRR014463X29930
4481
8887
588
82.2
globlastp

1
D1_P1

LAB736_H38
thellungiella_halophilum|11v
4482
8888
588
82.2
globlastp

2
1|BY802561_P1

LAB736_H38
thellungiella_halophilum|11v
4483
8889
588
82.2
globlastp

3
1|BY805880_P1

LAB736_H38
arabidopsis|10v1|AT2G47610
4484
8890
588
81.9
globlastp

4
_P1

LAB736_H38
b_juncea|12v1|E6ANDIZ01A
4485
8891
588
81.9
globlastp

5
9AN0_P1

LAB736_H38
canola|11v1|CN829492_P1
4486
8892
588
81.9
globlastp

6

LAB736_H38
cleome_gynandra|10v1|SRR0
4487
8893
588
81.9
globlastp

7
15532S0000623_P1

LAB736_H38
potato|10v1|BG351194_P1
4488
8894
588
81.9
globlastp

8

LAB736_H38
sarracenia|11v1|SRR192669.1
4489
8895
588
81.9
globlastp

9
62752XX1_P1

LAB736_H39
solanum_phureja|09v1|SPHB
4490
8894
588
81.9
globlastp

0
G126430_P1

LAB736_H39
strawberry|11v1|SRR034859S
4491
8896
588
81.9
globlastp

1
0009620_P1

LAB736_H39
thellungiella_halophilum|11v
4492
8897
588
81.9
globlastp

2
1|BY801480_P1

LAB736_H39
triphysaria|10v1|EY129416_P
4493
8898
588
81.9
globlastp

3
1

LAB736_H39
fraxinus|11v1|SRR058827.11
4494
8899
588
81.85
glotblastn

4
8836_T1

LAB736_H39
b_juncea|12v1|E6ANDIZ01A
4495
8900
588
81.5
globlastp

5
DW5C_P1

LAB736_H39
b_oleracea|gb161|AM385950
4496
8901
588
81.5
globlastp

6
_P1

LAB736_H39
b_rapa|11v1|CD813355_P1
4497
8900
588
81.5
globlastp

7

LAB736_H39
b_rapa|11v1|H07664_P1
4498
8901
588
81.5
globlastp

8

LAB736_H39
canola|11v1|CN731188_P1
4499
8901
588
81.5
globlastp

9

LAB736_H40
radish|gb164|EV527247_P1
4500
8902
588
81.5
globlastp

0

LAB736_H40
solanum_phureja|09v1|SPHB
4501
8903
588
81.5
globlastp

1
G131735_P1

LAB736_H40
tomato|11v1|BG126430_P1
4502
8904
588
81.5
globlastp

2

LAB736_H40
b_oleracea|gb161|DY027254_—
4503
8905
588
81.47
glotblastn

3
T1

LAB736_H40
fraxinus|11v1|SRR058827.10
4504
8906
588
81.47
glotblastn

4
7830_T1

LAB736_H40
fraxinus|11v1|SRR058827.10
4505
8907
588
81.47
glotblastn

5
8879XX1_T1

LAB736_H76
apple|11v1|MDP0000243029
4506
8908
588
81.2
globlastp

_P1

LAB736_H40
b_juncea|12v1|E6ANDIZ01A
4507
8909
588
81.1
globlastp

6
K52I_P1

LAB736_H40
b_juncea|12v1|E6ANDIZ01A
4508
8910
588
81.1
globlastp

7
SS71_P1

LAB736_H40
b_juncea|12v1|E6ANDIZ01B
4509
8911
588
81.1
globlastp

8
EY25_P1

LAB736_H40
b_juncea|12v1|E6ANDIZ01B
4510
8912
588
81.1
globlastp

9
IGDC_P1

LAB736_H41
b_oleracea|gb161|DY025922_—
4511
8913
588
81.1
globlastp

0
P1

LAB736_H41
b_rapa|11v1|CD817380_P1
4512
8914
588
81.1
globlastp

1

LAB736_H41
canola|11v1|CN726276_P1
4513
8915
588
81.1
globlastp

2

LAB736_H41
canola|11v1|CN730269_P1
4514
8915
588
81.1
globlastp

3

LAB736_H41
canola|11v1|CN732143_P1
4515
8913
588
81.1
globlastp

4

LAB736_H41
canola|11v1|CN735686_P1
4516
8916
588
81.1
globlastp

5

LAB736_H41
canola|11v1|DW999229_P1
4517
8915
588
81.1
globlastp

6

LAB736_H41
canola|11v1|EE474766_P1
4518
8915
588
81.1
globlastp

7

LAB736_H41
canola|11v1|H07610_P1
4519
8917
588
81.1
globlastp

8

LAB736_H41
canola|11v1|H07664_P1
4520
8918
588
81.1
globlastp

9

LAB736_H42
canola|11v1|SRR001111.1192
4521
8915
588
81.1
globlastp

0
0_P1

LAB736_H42
canola|11v1|SRR001111.1347
4522
8918
588
81.1
globlastp

1
3_P1

LAB736_H42
ginger|gb164|DY365119_P1
4523
8919
588
81.1
globlastp

2

LAB736_H42
iceplant|gb164|BE035811_P1
4524
8920
588
81.1
globlastp

3

LAB736_H42
radish|gb164|EV526734_P1
4525
8915
588
81.1
globlastp

4

LAB736_H42
radish|gb164|EV539295_P1
4526
8921
588
81.1
globlastp

5

LAB736_H42
radish|gb164|EW713801_P1
4527
8922
588
81.1
globlastp

6

LAB736_H42
radish|gb164|EW714466_P1
4528
8915
588
81.1
globlastp

7

LAB736_H42
safflower|gb162|EL403192_P
4529
8923
588
81.1
globlastp

8
1

LAB736_H42
b_juncea|12v1|E6ANDIZ01B
4530
8924
588
81.08
glotblastn

9
GI91_T1

LAB736_H43
fraxinus|11v1|SRR058827.10
4531
8925
588
81.08
glotblastn

0
6101_T1

LAB736_H43
ceratodon|10v1|SRR074890S
4532
8926
588
81
globlastp

1
0013604_P1

LAB736_H43
pteridium|11v1|SRR043594X
4533
8927
588
80.9
globlastp

2
107257_P1

LAB736_H43
pteridium|11v1|SRR043594X
4534
8928
588
80.9
globlastp

3
11299_P1

LAB736_H43
watermelon|11v1|VMEL0027
4535
8929
588
80.9
globlastp

4
1614810744_P1

LAB736_H43
pepper|12v1|BM059772_P1
4536
8930
588
80.8
globlastp

5

LAB736_H43
arabidopsis_lyrata|09v1|JGIA
4537
8931
588
80.7
globlastp

6
L019637_P1

LAB736_H43
arabidopsis|10v1|AT3G62870
4538
8932
588
80.7
globlastp

7
_P1

LAB736_H43
b_juncea|12v1|E6ANDIZ01A
4539
8933
588
80.7
globlastp

8
2BTE_P1

LAB736_H43
b_juncea|12v1|E6ANDIZ01A
4540
8934
588
80.7
globlastp

9
WD04_P1

LAB736_H44
b_oleracea|gb161|DY027226_—
4541
8935
588
80.7
globlastp

0
P1

LAB736_H44
b_rapa|11v1|CD815533_P1
4542
8936
588
80.7
globlastp

1

LAB736_H44
b_rapa|11v1|CX188832_P1
4543
8937
588
80.7
globlastp

2

LAB736_H44
b_rapa|11v1|H07610_P1
4544
8938
588
80.7
globlastp

3

LAB736_H44
canola|11v1|CN732426_P1
4545
8938
588
80.7
globlastp

4

LAB736_H44
canola|11v1|EE454032_P1
4546
8938
588
80.7
globlastp

5

LAB736_H44
canola|11v1|EE554163XX1_—
4547
8937
588
80.7
globlastp

6
P1

LAB736_H44
cynara|gb167|GE606374_P1
4548
8939
588
80.7
globlastp

7

LAB736_H44
flaveria|11v1|SRR149229.148
4549
8940
588
80.7
globlastp

8
826_P1

LAB736_H44
radish|gb164|EV525677_P1
4550
8941
588
80.7
globlastp

9

LAB736_H45
radish|gb164|EV541257_P1
4551
8942
588
80.7
globlastp

0

LAB736_H45
radish|gb164|EW717163_P1
4552
8943
588
80.7
globlastp

1

LAB736_H45
b_juncea|12v1|E6ANDIZ01A
4553
8944
588
80.69
glotblastn

2
8D7A_T1

LAB736_H45
flaveria|11v1|SRR149238.240
4554
8945
588
80.69
glotblastn

3
1_T1

LAB736_H45
physcomitrella|10v1|BQ0405
4555
8946
588
80.6
globlastp

4
18_P1

LAB736_H50
platanus|11v1|SRR096786X1
4556
8947
588
80.6
globlastp

35848_P1

LAB736_H45
cirsium|11v1|SRR346952.610
4557
8948
588
80.5
globlastp

5
836_P1

LAB736_H45
arabidopsis_lyrata|09v1|JGIA
4558
8949
588
80.31
glotblastn

6
L016184_T1

LAB736_H45
flaveria|11v1|SRR149229.379
4559
8950
588
80.31
glotblastn

7
853_T1

LAB736_H45
cannabis|12v1|JK493528_P1
4560
8951
588
80.3
globlastp

8

LAB736_H45
canola|11v1|EE460183_P1
4561
8952
588
80.3
globlastp

9

LAB736_H46
fraxinus|11v1|SRR058827.11
4562
8953
588
80.3
globlastp

0
248_P1

LAB736_H46
fraxinus|11v1|SRR058827.14
4563
8954
588
80.3
globlastp

1
3853_P1

LAB736_H46
radish|gb164|EV570087_P1
4564
8955
588
80.3
globlastp

2

LAB736_H46
silene|11v1|SRR096785X119
4565
8956
588
80.3
globlastp

3
902_P1

LAB736_H46
thellungiella_parvulum|11v1|
4566
8957
588
80.3
globlastp

4
BY801481_P1

LAB736_H46
marchantia|gb166|AU081821
4567
8958
588
80.2
globlastp

5
_P1

LAB738, LAB
rice|11v1|AA751333
4568
8959
589
99.9
globlastp

738_H1

LAB738_H2
barley|10v2|AV833411
4569
8960
589
96.4
globlastp

LAB738_H2
barley|12v1|AV833411_P1
4570
8960
589
96.4
globlastp

LAB738_H3
brachypodium|12v1|BRADI1
4571
8961
589
96.3
globlastp

G35960_P1

LAB738_H4
rye|12v1|DRR001012.111848
4572
8962
589
96.3
globlastp

LAB738_H5
rye|12v1|DRR001012.116924
4573
8962
589
96.3
globlastp

LAB738_H6
wheat|10v2|BE423931
4574
8963
589
96.1
globlastp

LAB738_H6
wheat|12v3|BE423931_P1
4575
8963
589
96.1
globlastp

LAB738_H94
wheat|12v3|AL818007_P1
4576
8964
589
96
globlastp

LAB738_H95
wheat|12v3|CV066767_P1
4577
8965
589
96
globlastp

LAB738_H7
sugarcane|10v1|CA074185
4578
8966
589
95.2
globlastp

LAB738_H8
sorghum|12v1|SB10G024200
4579
8967
589
95.1
globlastp

LAB738_H96
switchgrass|12v1|FL697837_—
4580
8968
589
94.7
globlastp

P1

LAB738_H97
switchgrass|12v1|PV12v1PR
4581
8968
589
94.7
globlastp

D050484_P1

LAB738_H11
switchgrass|12v1|FE599555_—
4582
8969
589
94.5
globlastp

P1

LAB738_H9
foxtail_millet|11v3|PHY7SI0
4583
8970
589
94.2
globlastp

05897M_P1

LAB738_H10
foxtail_millet|11v3|SICRP101
4584
8970
589
94.2
globlastp

553_P1

LAB738_H11
switchgrass|gb167|FE599555
4585
8971
589
94.2
globlastp

LAB738_H12
millet|10v1|EVO454PM0359
4586
8972
589
93.9
globlastp

93_P1

LAB738_H13
maize|10v1|AI691519_P1
4587
8973
589
93.5
globlastp

LAB738_H14
maize|10v1|AW171782_P1
4588
8974
589
93.2
globlastp

LAB738_H98
banana|12v1|MAGEN201202
4589
8975
589
89.3
globlastp

7451_P1

LAB738_H15
oil_palm|11v1|EL685913_P1
4590
8976
589
89.2
globlastp

LAB738_H16
aristolochia|10v1|FD749188_—
4591
8977
589
85.3
globlastp

P1

LAB738_H17
clementine|11v1|CF828766_P
4592
8978
589
85.1
globlastp

1

LAB738_H18
orange|11v1|CF828766_P1
4593
8978
589
85.1
globlastp

LAB738_H19
poplar|10v1|BI135550
4594
8979
589
84.92
glotblastn

LAB738_H20
phalaenopsis|11v1|SRR12577
4595
8980
589
84.9
globlastp

1.1009671_P1

LAB738_H21
poplar|10v1|BI138396
4596
8981
589
84.9
globlastp

LAB738_H19,
poplar|13v1|BI135550_P1
4597
8981
589
84.9
globlastp

LAB738_H21

LAB738_H22
amorphophallus|11v2|SRR08
4598
8982
589
84.66
glotblastn

9351X379368_T1

LAB738_H23
vinca|11v1|SRR098690X1345
4599
8983
589
84.66
glotblastn

08

LAB738_H99
castorbean|12v1|EE258884_T
4600
8984
589
84.54
glotblastn

1

LAB738_H24
apple|11v1|CN491356_P1
4601
8985
589
84.5
globlastp

LAB738_H10
aquilegia|10v2|DT746718_P1
4602
8986
589
84.3
globlastp

0

LAB738_H10
sesame|12v1|SESI12V126493
4603
8987
589
84.3
globlastp

1
6_P1

LAB738_H25
tabernaemontana|11v1|SRR09
4604
8988
589
84.3
globlastp

8689X112973

LAB738_H26
cotton|11v1|CO079317_P1
4605
8989
589
84.1
globlastp

LAB738_H27
euphorbia|11v1|SRR098678X
4606
8990
589
84.1
globlastp

104741_P1

LAB738_H28
vinca|11v1|SRR098690X1039
4607
8991
589
84.1
globlastp

65

LAB738_H29
cotton|11v1|AI726119_T1
4608
8992
589
84.02
glotblastn

LAB738_H10
bean|12v2|SRR001334.13297
4609
8993
589
84
globlastp

2
8_P1

LAB738_H10
prunus_mume|13v1|BU04086
4610
8994
589
84
globlastp

3
5_P1

LAB738_H31
cacao|10v1|CU484549_P1
4611
8995
589
84
globlastp

LAB738_H32
cucumber|09v1|BGI454G004
4612
8996
589
84
globlastp

6050_P1

LAB738_H33
gossypium_raimondii|12v1|AI
4613
8997
589
84
globlastp

726119_P1

LAB738_H34
grape|11v1|DV221675_P1
4614
8998
589
84
globlastp

LAB738_H35
grape|11v1|GSVIVT0103352
4615
8998
589
84
globlastp

9001_P1

LAB738_H36
tomato|11v1|CK266233
4616
8999
589
84
globlastp

LAB738_H43
poplar|13v1|AI166027_P1
4617
9000
589
84
globlastp

LAB738_H37
arabidopsis|10v1|AT3G57880
4618
9001
589
83.9
globlastp

_P1

LAB738_H38
plantago|11v2|SRR066373X1
4619
9002
589
83.9
globlastp

1771_P1

LAB738_H39
watermelon|11v1|VMEL0487
4620
9003
589
83.9
globlastp

5107700323

LAB738_H40
arabidopsis_lyrata|09v1|JGIA
4621
9004
589
83.8
globlastp

L019081_P1

LAB738_H41
eucalyptus|11v2|ES596977_P
4622
9005
589
83.8
globlastp

1

LAB738_H42
flaveria|11v1|SRR149229.933
4623
9006
589
83.8
globlastp

24_P1

LAB738_H43
poplar|10v1|AI166027
4624
9007
589
83.8
globlastp

LAB738_H44
solanum_phureja|09v1|SPHC
4625
9008
589
83.8
globlastp

K266233

LAB738_H45
tripterygium|11v1|SRR09867
4626
9009
589
83.8
globlastp

7X101001

LAB738_H46
tomato|11v1|AI486042
4627
9010
589
83.7
globlastp

LAB738_H47
beech|11v1|SRR006293.3256
4628
9011
589
83.66
glotblastn

3_T1

LAB738_H48
pigeonpea|11v1|SRR054580X
4629
9012
589
83.6
globlastp

105301_P1

LAB738_H10
bean|12v2|CA913595_P1
4630
9013
589
83.5
globlastp

4

LAB738_H10
nicotiana_benthamiana|12v1|
4631
9014
589
83.5
globlastp

5
EH370031_P1

LAB738_H49
bean|12v1|CA913595
4632
9013
589
83.5
globlastp

LAB738_H50
cucumber|09v1|AM714320_P
4633
9015
589
83.5
globlastp

1

LAB738_H51
pigeonpea|11v1|CCIIPG1102
4634
9016
589
83.5
globlastp

9439_P1

LAB738_H52
pigeonpea|11v1|SRR054580X
4635
9016
589
83.5
globlastp

127989_P1

LAB738_H53
thellungiella_halophilum|11v
4636
9017
589
83.5
globlastp

1|DN774551

LAB738_H54
thellungiella_halophilum|11v
4637
9017
589
83.5
globlastp

1|EHCRP065122

LAB738_H10
blueberry|12v1|SRR353282X
4638
9018
589
83.4
glotblastn

6
25634D1_T1

LAB738_H55
strawberry|11v1|DY674884
4639
9019
589
83.4
globlastp

LAB738_H56
watermelon|11v1|AM714320
4640
9020
589
83.4
globlastp

LAB738_H57
beet|12v1|BQ583781_P1
4641
9021
589
83.3
globlastp

LAB738_H58
monkeyflower|10v1|DV20774
4642
9022
589
83.3
globlastp

2

LAB738_H58
monkeyflower|12v1|DV20774
4643
9022
589
83.3
globlastp

2_P1

LAB738_H59
valeriana|11v1|SRR099039X1
4644
9023
589
83.3
globlastp

1064

LAB738_H60
silene|11v1|SRR096785X105
4645
9024
589
83.2
globlastp

314

LAB738_H10
zostera|12v1|SRR057351X10
4646
9025
589
83.1
globlastp

7
1106D1_P1

LAB738_H61
zostera|10v1|SRR057351S000
4647
9025
589
83.1
globlastp

9902

LAB738_H10
aquilegia|10v2|DR913521_P1
4648
9026
589
83
globlastp

8

LAB738_H62
amborella|12v3|SRR038634.1
4649
9027
589
83
globlastp

6406_P1

LAB738_H63
b_rapa|11v1|CD813540_P1
4650
9028
589
83
globlastp

LAB738_H64
soybean|11v1|GLYMA19G32
4651
9029
589
83
globlastp

730

LAB738_H64
soybean|12v1|GLYMA19G32
4652
9029
589
83
globlastp

730_P1

LAB738_H65
thellungiella_parvulum|11v1|
4653
9030
589
83
globlastp

DN774551

LAB738_H10
banana|12v1|MAGEN201202
4654
9031
589
82.9
globlastp

9
0855_P1

LAB738_H66
medicago|12v1|BG448505_P
4655
9032
589
82.9
globlastp

1

LAB738_H67
soybean|11v1|GLYMA03G29
4656
9033
589
82.9
globlastp

840

LAB738_H67
soybean|12v1|GLYMA03G29
4657
9033
589
82.9
globlastp

840T3_P1

LAB738_H68
chickpea|11v1|GR914055
4658
9034
589
82.8
globlastp

LAB738_H68
chickpea|13v2|GR914055_P1
4659
9034
589
82.8
globlastp

LAB738_H69
sunflower|12v1|DY904454
4660
9035
589
82.7
globlastp

LAB738_H70
poppy|11v1|SRR030259.1209
4661
9036
589
82.6
globlastp

54_P1

LAB738_H71
canola|11v1|EE553143_P1
4662
9037
589
82.5
globlastp

LAB738_H72
solanum_phureja|09v1|SPHC
4663
9038
589
82.5
globlastp

RPSP045467

LAB738_H73
tomato|11v1|FN000203
4664
9039
589
82.5
globlastp

LAB738_H11
lettuce|12v1|CV699924_P1
4665
9040
589
82.4
globlastp

0

LAB738_H74
b_rapa|11v1|CD826734_P1
4666
9041
589
82.4
globlastp

LAB738_H75
amorphophallus|11v2|SRR08
4667
9042
589
82.3
globlastp

9351X104690_P1

LAB738_H76
chickpea|11v1|SRR133517.11
4668
9043
589
82.1
globlastp

0375

LAB738_H76
chickpea|13v2|SRR133517.11
4669
9043
589
82.1
globlastp

0375_P1

LAB738_H77
nasturtium|11v1|SRR032558.
4670
9044
589
81.8
globlastp

127153_P1

LAB738_H78
orobanche|10v1|SRR023189S
4671
9045
589
81.8
globlastp

0037981_P1

LAB738_H11
nicotiana_benthamiana|12v1|
4672
9046
589
81.7
globlastp

1
FG132662_P1

LAB738_H79
prunus|10v1|BU040865
4673
9047
589
81.7
globlastp

LAB738_H11
nicotiana_benthamiana|12v1|
4674
9048
589
81.6
globlastp

2
EB434069_P1

LAB738_H11
lettuce|12v1|DY960088_P1
4675
9049
589
81.5
globlastp

3

LAB738_H80
cotton|11v1|AI728115_P1
4676
9050
589
81.5
globlastp

LAB738_H81
cotton|11v1|BG440202_P1
4677
9051
589
81.5
globlastp

LAB738_H82
gossypium_raimondii|12v1|AI
4678
9051
589
81.5
globlastp

728115_P1

LAB738_H83
arabidopsis_lyrata|09v1|JGIA
4679
9052
589
81.4
globlastp

L004632_P1

LAB738_H84
eschscholzia|11v1|SRR01411
4680
9053
589
81.4
glotblastn

6.104771_T1

LAB738_H85
cacao|10v1|CU471395_P1
4681
9054
589
81.3
globlastp

LAB738_H86
soybean|11v1|GLYMA10G11
4682
9055
589
81.2
globlastp

910

LAB738_H86
soybean|12v1|GLYMA10G11
4683
9055
589
81.2
globlastp

910_P1

LAB738_H11
nicotiana_benthamiana|12v1|
4684
9056
589
81.1
globlastp

4
NB12v1CRP063262_P1

LAB738_H87
orange|11v1|BQ623995_P1
4685
9057
589
81.1
globlastp

LAB738_H88
thellungiella_halophilum|11v
4686
9058
589
81
globlastp

1|EHJGI11005539

LAB738_H89
clementine|11v1|BQ623995_—
4687
9059
589
80.9
globlastp

P1

LAB738_H90
arabidopsis|10v1|AT1G51570
4688
9060
589
80.8
globlastp

_P1

LAB738_H91
gossypium_raimondii|12v1|D
4689
9061
589
80.4
globlastp

T544433_P1

LAB738_H92
cotton|11v1|CO108032_P1
4690
9062
589
80.3
globlastp

LAB738_H93
ambrosia|11v1|SRR346935.1
4691
9063
589
80.2
globlastp

03505_P1

LAB740_H1
sorghum|12v1|SB03G000860
4692
9064
591
90.3
globlastp

LAB740_H2
maize|10v1|AW438269_T1
4693
9065
591
89.16
glotblastn

LAB740_H3
leymus|gb166|EG375183_P1
4694
9066
591
80.4
globlastp

LAB740_H4
wheat|10v2|BE492719
4695
9067
591
80.4
globlastp

LAB741_H1
maize|10v1|AI947275_P1
4696
9068
592
92.2
globlastp

LAB741_H2
foxtail_millet|11v3|PHY7SI0
4697
9069
592
87.6
globlastp

01484M_P1

LAB741_H3
switchgrass|12v1|GD020272_—
4698
9070
592
85.6
globlastp

P1

LAB741_H4
switchgrass|12v1|GD032484_—
4699
9071
592
85.2
globlastp

P1

LAB744_H3
switchgrass|12v1|FL909921_—
4700
9072
594
87.13
glotblastn

T1

LAB744_H5
switchgrass|12v1|HO323691_—
4701
9073
594
86.14
glotblastn

T1

LAB744_H2
millet|10v1|EVO454PM0862
4702
9074
594
86.14
glotblastn

63_T1

LAB744_H3
switchgrass|gb167|FL909921
4703
9075
594
86.14
glotblastn

LAB744_H4
foxtail_millet|11v3|PHY7SI0
4704
9076
594
85.15
glotblastn

01466M_T1

LAB748_H1
sugarcane|10v1|CA121279
4705
596
596
100
globlastp

LAB748_H2
maize|10v1|AW054474_P1
4706
9077
596
97.6
globlastp

LAB748_H3
foxtail_millet|11v3|PHY7SI0
4707
9078
596
96.4
globlastp

37580M_P1

LAB748_H4
maize|10v1|AW147094_P1
4708
9079
596
95.2
globlastp

LAB748_H5
millet|10v1|EVO454PM0344
4709
9080
596
95.2
globlastp

30_P1

LAB748_H6
wheat|10v2|CA617591
4710
9079
596
95.2
globlastp

LAB748_H7
rice|11v1|GFXAC091247X34
4711
9081
596
92.8
globlastp

LAB748_H8
oat|11v1|GO581442_P1
4712
9082
596
91.7
globlastp

LAB748_H9
fescue|gb161|DT697589_P1
4713
9083
596
90.5
globlastp

LAB748_H10
brachypodium|12v1|BRANDI1
4714
9084
596
89.2
globlastp

G03410_P1

LAB748_H11
switchgrass|gbl67|FL840000
4715
9085
596
89.2
globlastp

LAB748_H11
switchgrass|12v1|DN141373_—
4716
9086
596
88
globlastp

P1

LAB748_H12
lovegrass|gb167|EH186314_P
4717
9087
596
88
globlastp

1

LAB748_H13
foxtail_millet|11v3|PHY7SI0
4718
9088
596
86.7
globlastp

31695M_P1

LAB748_H14
millet|10v1|EVO454PM0328
4719
9089
596
86.7
globlastp

37_P1

LAB748_H15
switchgrass|12v1|FE617806_—
4720
9090
596
85.5
globlastp

P1

LAB748_H15
switchgrass|gb167|FE617806
4721
9090
596
85.5
globlastp

LAB748_H29
banana|12v1|MAGEN201201
4722
9091
596
84.7
globlastp

8813_P1

LAB748_H16
oil_palm|11v1|EY397345_P1
4723
9092
596
84.5
globlastp

LAB748_H17
epimedium|11v1|SRR013502.
4724
9093
596
83.3
globlastp

17003_P1

LAB748_H18
avocado|10v1|CV459198_P1
4725
9094
596
82.1
globlastp

LAB748_H19
switchgrass|12v1|FE656831_—
4726
9095
596
81.93
glotblastn

T1

LAB748_H19
switchgrass|gb167|FE656831
4727
9096
596
81.9
globlastp

LAB748_H30
zostera|12v1|SRR057351X12
4728
9097
596
81
globlastp

2968D1_P1

LAB748_H20
liriodendron|gb166|FD488260
4729
9098
596
81
globlastp

_P1

LAB748_H21
oil_palm|11v1|SRR190698.14
4730
9099
596
81
globlastp

1100XX1_P1

LAB748_H22
zostera|10v1|SRR057351S002
4731
9097
596
81
globlastp

4218

LAB748_H23
amborella|12v3|CK762188_T
4732
9100
596
80.95
glotblastn

1

LAB748_H24
poppy|11v1|SRR030259.1009
4733
9101
596
80.95
glotblastn

38_T1

LAB748_H25
poppy|11v1|SRR030259.1892
4734
9101
596
80.95
glotblastn

87_T1

LAB748_H26
rice|11v1|CV730687
4735
9102
596
80
globlastp

LAB748_H27
sarracenia|11v1|SRR192669.1
4736
9103
596
80
globlastp

09235

LAB748_H28
sunflower|12v1|DY955975
4737
9104
596
80
globlastp

LAB749_H2
foxtail_millet|11v3|PHY7SI0
4738
9105
597
81.3
globlastp

36600M_P1

LAB750_H3
switchgrass|gb167|DN149820
4739
9106
598
85.75
glotblastn

LAB750_H3
switchgrass|12v1|DN149820_—
4740
9107
598
85.4
globlastp

P1

LAB752_H1
maize|10v1|AI795704_P1
4741
9108
600
97.1
globlastp

LAB752_H2
foxtail_millet|11v3|PHY7SI0
4742
9109
600
94
globlastp

16661M_P1

LAB752_H3
millet|10v1|EVO454PM0030
4743
9110
600
89.3
globlastp

18_P1

LAB752_H4
rice|11v1|CB630474
4744
9111
600
88.2
globlastp

LAB752_H5
rice|11v1|HS335780
4745
9112
600
87.82
glotblastn

LAB752_H6
rye|12v1|DRR001012.105294
4746
9113
600
85.3
globlastp

LAB752_H7
brachypodium|12v1|BRADI3
4747
9114
600
84.8
globlastp

G03990_P1

LAB752_H8
wheat|10v2|BE418284
4748
9115
600
84.8
globlastp

LAB753_H2
foxtail_millet|11v3|PHY7SI0
4749
9116
601
83.3
globlastp

34194M_P1

LAB753_H3
switchgrass|12v1|SRR187770
4750
9117
601
81.5
globlastp

.885396_P1

LAB755_H1
sugarcane|10v1|CA072083
4751
9118
603
99
globlastp

LAB755_H2
maize|10v1|AI891244_P1
4752
9119
603
97.7
globlastp

LAB755_H3
maize|10v1|BG319910_P1
4753
9120
603
97.4
globlastp

LAB755_H19
switchgrass|12v1|FE601494_—
4754
9121
603
95.7
globlastp

P1

LAB755_H4
foxtail_millet|11v3|EC612069
4755
9122
603
95.7
globlastp

_P1

LAB755_H5
millet|10v1|EVO454PM0175
4756
9123
603
95.7
globlastp

12_P1

LAB755_H6
switchgrass|gb167|FE601494
4757
9121
603
95.7
globlastp

LAB755_H7
cenchrus|gb166|EB662143_P
4758
9124
603
95.4
globlastp

1

LAB755_H8
switchgrass|gb167|FE630750
4759
9125
603
95.4
globlastp

LAB755_H20
switchgrass|12v1|FE630750_—
4760
9126
603
95.38
glotblastn

T1

LAB755_H9
wheat|10v2|BE401944
4761
9127
603
93.4
globlastp

LAB755_H9
wheat|12v3|BE401262_P1
4762
9127
603
93.4
globlastp

LAB755_H10
rye|12v1|BE586997
4763
9128
603
93.1
globlastp

LAB755_H11
barley|10v2|BI950683
4764
9129
603
92.4
globlastp

LAB755_H12
brachypodium|12v1|BRADI1
4765
9130
603
92.4
globlastp

G56720_P1

LAB755_H13
rye|12v1|DRR001012.129717
4766
9131
603
91.42
glotblastn

LAB755_H14
rice|11v1|BF430488
4767
9132
603
91.4
globlastp

LAB755_H21
wheat|12v3|BE516195_T1
4768
9133
603
88.45
glotblastn

LAB755_H15
oat|11v1|GO589959_P1
4769
9134
603
86.8
globlastp

LAB755_H16
leymus|gb166|EG375676_P1
4770
9135
603
85.8
globlastp

LAB755_H17
rye|12v1|BE705438
4771
9136
603
85.5
globlastp

LAB755_H18
rye|12v1|DRR001013.103314
4772
9137
603
85.2
glotblastn

LAB755_H22
barley|12v1|BG418473_P1
4773
9138
603
82.1
globlastp

LAB755_H11
barley|12v1|BF259611_P1
4774
9139
603
82
globlastp

LAB756_H1
maize|10v1|BE509971_P1
4775
9140
604
95.2
globlastp

LAB756_H2
foxtail_millet|11v3|PHY7SI0
4776
9141
604
92.7
globlastp

29403M_P1

LAB756_H9
wheat|12v3|CA698703_P1
4777
9142
604
86.2
globlastp

LAB756_H10
wheat|12v3|BE500675_P1
4778
9143
604
86
globlastp

LAB756_H4
wheat|10v2|BE500675
4779
9144
604
85.6
globlastp

LAB756_H4
wheat|12v3|BF482273_P1
4780
9144
604
85.6
globlastp

LAB756_H5
barley|10v2|BF625584
4781
9145
604
85.5
globlastp

LAB756_H5
barley|12v1|BF625584_P1
4782
9145
604
85.5
globlastp

LAB756_H6
rye|12v1|BE637164
4783
9146
604
84.9
globlastp

LAB756_H7
brachypodium|12v1|BRADI2
4784
9147
604
84.6
globlastp

G36950_P1

LAB756_H11
switchgrass|12v1|FE611271_—
4785
9148
604
82.16
glotblastn

T1

LAB756_H8
oat|11v1|GR314603_T1
4786
9149
604
82.01
glotblastn

LAB757_H1
maize|10v1|BU098544_P1
4787
9150
605
92.1
globlastp

LAB757_H4
brachypodium|12v1|BRADI4
4788
9151
605
83
globlastp

G33500_P1

LAB758_H1
maize|10v1|AI943758_P1
4789
9152
606
88.3
globlastp

LAB758_H2
sorghum|12v1|SB01G022280
4790
9153
606
84.8
globlastp

LAB758_H3
maize|10v1|AI586577_P1
4791
9154
606
83.3
globlastp

LAB758_H4
switchgrass|gb167|FE611481
4792
9155
606
82.3
globlastp

LAB759_H1
sugarcane|10v1|CF569861
4793
9156
607
99.2
globlastp

LAB759_H2
maize|10v1|BG840584_P1
4794
9157
607
96.2
globlastp

LAB759_H6
switchgrass|12v1|FL762763_—
4795
9158
607
94.7
globlastp

P1

LAB759_H3
foxtail_millet|11v3|PHY7SI0
4796
9159
607
94.7
globlastp

31493M_P1

LAB759_H4
millet|10v1|PMSLX0019079
4797
9159
607
94.7
globlastp

D1_P1

LAB759_H5
switchgrass|12v1|FL788295_—
4798
9158
607
94.7
globlastp

P1

LAB759_H5
switchgrass|gb167|FL762763
4799
9158
607
94.7
globlastp

LAB760_H1
sugarcane|10v1|CA177853
4800
9160
608
94.3
globlastp

LAB760_H2
sugarcane|10v1|CA077053
4801
9161
608
93
globlastp

LAB760_H8
switchgrass|12v1|DN140645_—
4802
9162
608
92.4
globlastp

P1

LAB760_H3
switchgrass|12v1|DN143706_—
4803
9163
608
92
globlastp

P1

LAB760_H3
switchgrass|gb167|DN143706
4804
9163
608
92
globlastp

LAB760_H4
maize|10v1|ZMU17350_P1
4805
9164
608
91.9
globlastp

LAB760_H5
maize|10v1|ZMU17351_P1
4806
9165
608
91.9
globlastp

LAB760_H6
foxtail_millet|11v3|PHY7SI0
4807
9166
608
91.5
globlastp

06473M_P1

LAB760_H7
cenchrus|gb166|EB653187_P
4808
9167
608
91.2
globlastp

1

LAB760_H8
switchgrass|gb167|DN140645
4809
9168
608
91
globlastp

LAB760_H9
sorghum|12v1|SB03G025520
4810
9169
608
90.8
globlastp

LAB760_H10
foxtail_millet|11v3|PHY7SI0
4811
9170
608
88.4
globlastp

30346M_P1

LAB760_H11
pseudoroegneria|gb167|FF350
4812
9171
608
87.8
globlastp

486

LAB760_H12
fescue|gb161|DT679269_P1
4813
9172
608
87.1
globlastp

LAB760_H13
oat|11v1|GR318697_P1
4814
9173
608
87.1
globlastp

LAB760_H14
oat|11v1|CN817771_P1
4815
9174
608
86.8
globlastp

LAB760_H15
oat|11v1|GR315717_P1
4816
9174
608
86.8
globlastp

LAB760_H16
oat|11v1|CN819042_P1
4817
9175
608
86.5
globlastp

LAB760_H17
brachypodium|12v1|BRADI1
4818
9176
608
86.3
globlastp

G25860_P1

LAB760_H18
barley|10v2|BE413112
4819
9177
608
86.2
globlastp

LAB760_H18
barley|12v1|BE413112_P1
4820
9177
608
86.2
globlastp

LAB760_H19
leymus|gb166|CD808458_P1
4821
9178
608
86
globlastp

LAB760_H20
pseudoroegneria|gb167|FF340
4822
9179
608
86
globlastp

160

LAB760_H21
rye|12v1|BE704669
4823
9180
608
86
globlastp

LAB760_H22
wheat|10v2|BE216960
4824
9181
608
86
globlastp

LAB760_H22
wheat|12v3|BE216960_P1
4825
9181
608
86
globlastp

LAB760_H23
rice|11v1|AA753367
4826
9182
608
85.8
globlastp

LAB761_H1
maize|10v1|AI901825_P1
4827
9183
609
95
globlastp

LAB761_H2
switchgrass|12v1|FL804327_—
4828
9184
609
93.9
globlastp

P1

LAB761_H2
switchgrass|gb167|FE620812
4829
9185
609
93.6
globlastp

LAB761_H3
sugarcane|10v1|CA073413
4830
9186
609
88.3
globlastp

LAB761_H4
rice|11v1|BI807111
4831
9187
609
86.9
globlastp

LAB761_H5
brachypodium|12v1|BRADI2
4832
9188
609
86.1
globlastp

G03370_P1

LAB761_H7
wheat|12v3|BE637769_P1
4833
9189
609
83.8
globlastp

LAB761_H6
rye|12v1|DRR001012.319088
4834
9190
609
83.6
globlastp

LAB761_H7
wheat|10v2|BE637769
4835
9191
609
83.6
globlastp

LAB761_H8
rye|12v1|DRR001012.129842
4836
9192
609
83.3
globlastp

LAB761_H9
rye|12v1|DRR001012.202045
4837
9193
609
83.3
globlastp

LAB761_H10
rye|12v1|DRR001012.363435
4838
9194
609
83.29
glotblastn

LAB761_H11
rye|12v1|DRR001013.43321
4839
9195
609
80.94
glotblastn

LAB761_H12
foxtail_millet|11v3|PHY7SI0
4840
9196
609
80.8
globlastp

02366M_P1

LAB761_H13
switchgrass|12v1|FE600995_—
4841
9197
609
80.2
globlastp

P1

LAB762_H1
sugarcane|10v1|CA094266
4842
9198
610
98.6
globlastp

LAB762_H2
maize|10v1|AI901314_P1
4843
9199
610
94.9
globlastp

LAB762_H3
foxtail_millet|11v3|PHY7SI0
4844
9200
610
93.2
globlastp

02044M_P1

LAB762_H10
switchgrass|12v1|FL817879_—
4845
9201
610
92.7
globlastp

P1

LAB762_H11
switchgrass|12v1|FL851031_—
4846
9202
610
92.7
globlastp

P1

LAB762_H4
millet|10v1|EVO454PM0454
4847
9203
610
92.4
globlastp

29_P1

LAB762_H5
rice|11v1|AU058134
4848
9204
610
87.9
globlastp

LAB762_H6
brachypodium|12v1|BRADI2
4849
9205
610
85.6
globlastp

G42932_P1

LAB762_H7
rye|12v1|DRR001012.137316
4850
9206
610
85.6
globlastp

LAB762_H8
barley|10v2|AJ485891
4851
9207
610
85
globlastp

LAB762_H12
wheat|12v3|BE402159_P1
4852
9208
610
84.7
globlastp

LAB762_H9
wheat|10v2|BE402159
4853
9209
610
84.7
globlastp

LAB763_H1
maize|10v1|BG349143_P1
4854
9210
611
97.1
globlastp

LAB763_H2
maize|10v1|CD998134_P1
4855
9211
611
95.4
globlastp

LAB763_H8
switchgrass|12v1|DN151198_—
4856
9212
611
92.1
globlastp

P1

LAB763_H3
foxtail_millet|11v3|PHY7SI0
4857
9213
611
91.6
globlastp

02741M_P1

LAB763_H4
millet|10v1|EVO454PM0216
4858
9214
611
90.8
globlastp

69_P1

LAB763_H5
rice|11v1|CB483512
4859
9215
611
90.8
globlastp

LAB763_H6
rice|11v1|BI796638
4860
9216
611
90
globlastp

LAB763_H7
switchgrass|gb167|FE605980
4861
9217
611
89.17
glotblastn

LAB763_H7
switchgrass|12v1|FE605980_—
4862
9218
611
88.75
glotblastn

T1

LAB763_H8
switchgrass|gb167|DN151198
4863
9219
611
88.7
globlastp

LAB763_H9
brachypodium|12v1|BRADI2
4864
9220
611
86.2
globlastp

G45690T2_P1

LAB763_H10
leymus|gb166|EG374812_P1
4865
9221
611
85.4
globlastp

LAB763_H11
wheat|10v2|BE406566
4866
9222
611
84.9
globlastp

LAB763_H11
wheat|12v3|BE406566_P1
4867
9222
611
84.9
globlastp

LAB763_H12
rye|12v1|DRR001012.117963
4868
9223
611
84.5
globlastp

LAB763_H13
barley|10v2|BF621257
4869
9224
611
84.1
globlastp

LAB763_H13
barley|12v1|BF621257_P1
4870
9224
611
84.1
globlastp

LAB763_H14
oat|11v1|GO592659_P1
4871
9225
611
83.7
globlastp

LAB764_H1
maize|10v1|AI491605_P1
4872
9226
612
96.8
globlastp

LAB764_H2
foxtail_millet|11v3|PHY7SI0
4873
9227
612
95.5
globlastp

01129M_P1

LAB764_H3
millet|10v1|EVO454PM0347
4874
9228
612
92.7
globlastp

17_P1

LAB764_H19
switchgrass|12v1|FE622905_—
4875
9229
612
91.78
glotblastn

T1

LAB764_H4
rice|11v1|AA750113
4876
9230
612
90.7
globlastp

LAB764_H5
brachypodium|12v1|BRADI2
4877
9231
612
84.3
globlastp

G49460_P1

LAB764_H6
millet|10v1|CD724502_P1
4878
9232
612
84
globlastp

LAB764_H7
foxtail_millet|11v3|PHY7SI0
4879
9233
612
83.6
globlastp

21809M_P1

LAB764_H8
maize|10v1|AI677105_P1
4880
9234
612
83.6
globlastp

LAB764_H9
sorghum|12v1|SB09G026080
4881
9235
612
83.6
globlastp

LAB764_H20
switchgrass|12v1|DN147696_—
4882
9236
612
83.4
globlastp

P1

LAB764_H21
switchgrass|12v1|FE652151_—
4883
9237
612
83.2
globlastp

P1

LAB764_H10
rye|12v1|DRR001012.107471
4884
9238
612
83.2
globlastp

LAB764_H11
barley|10v2|BF255578
4885
9239
612
83
globlastp

LAB764_H11
barley|12v1|BI950337_P1
4886
9239
612
83
globlastp

LAB764_H12
leymus|gb166|EG375107_P1
4887
9240
612
83
globlastp

LAB764_H13
brachypodium|12v1|BRADI2
4888
9241
612
82.9
globlastp

G19390_P1

LAB764_H14
maize|10v1|AI612416_P1
4889
9242
612
81.7
globlastp

LAB764_H15
rice|11v1|AA753037
4890
9243
612
81.6
globlastp

LAB764_H16
wheat|10v2|BE404250XX1
4891
9244
612
81.6
globlastp

LAB764_H22
switchgrass|12v1|FL699860_—
4892
9245
612
81.4
globlastp

P1

LAB764_H16
wheat|12v3|BE404250_T1
4893
9246
612
81.25
glotblastn

LAB764_H17
switchgrass|gb167|FE622905
4894
9247
612
81.2
globlastp

LAB765_H1
sugarcane|10v1|CA071302
4895
9248
613
98
globlastp

LAB765_H2
maize|10v1|AW066821_P1
4896
9249
613
95.2
globlastp

LAB765_H4, L
switchgrass|12v1|FE601807_—
4897
9250
613
94
globlastp

AB765_H5
P1

LAB765_H3
foxtail_millet|11v3|PHY7SI0
4898
9251
613
93.8
globlastp

01717M_P1

LAB765_H4
switchgrass|gb167|FE601808
4899
9252
613
93.8
globlastp

LAB765_H5
switchgrass|gb167|FE601807
4900
9253
613
93
globlastp

LAB765_H6
millet|10v1|EVO454PM0122
4901
9254
613
92.8
globlastp

48_P1

LAB765_H7
rice|11v1|AF071063
4902
9255
613
90.5
globlastp

LAB765_H8
rice|11v1|CK043743
4903
9256
613
90
glotblastn

LAB765_H9
maize|10v1|AI714826_P1
4904
9257
613
89.9
globlastp

LAB765_H20
switchgrass|12v1|FE636720_—
4905
9258
613
87.9
globlastp

P1

LAB765_H10
brachypodium|12v1|BRADI2
4906
9259
613
85.5
globlastp

G50250_P1

LAB765_H11
fescue|gb161|DT675095_P1
4907
9260
613
84.3
globlastp

LAB765_H12
leymus|gb166|EG375598_P1
4908
9261
613
83.2
globlastp

LAB765_H13
barley|10v2|BE422148
4909
9262
613
83
globlastp

LAB765_H13
barley|12v1|BE422148_P1
4910
9263
613
82.8
globlastp

LAB765_H14
wheat|10v2|BE415693
4911
9264
613
82.8
globlastp

LAB765_H14,
wheat|12v3|BE415693_P1
4912
9264
613
82.8
globlastp

LAB765_H17

LAB765_H15
rye|12v1|BE495414
4913
9265
613
82.6
globlastp

LAB765_H16
rye|12v1|BE704883
4914
9266
613
81.8
globlastp

LAB765_H17
wheat|10v2|BE489787
4915
9267
613
81.8
globlastp

LAB765_H18
rye|12v1|DRR001012.200584
4916
9268
613
81.75
glotblastn

LAB765_H19
rye|12v1|DRR001012.264867
4917
9269
613
81.5
globlastp

LAB767_H1
maize|10v1|AW331209_P1
4918
9270
614
89.9
globlastp

LAB767_H2
foxtail_millet|11v3|PHY7SI0
4919
9271
614
89.1
globlastp

02131M_P1

LAB767_H3
switchgrass|12v1|FL851255_—
4920
9272
614
87.5
globlastp

P1

LAB769_H1
maize|10v1|CO522797_P1
4921
9273
616
92.3
globlastp

LAB769_H2
foxtail_millet|11v3|PHY7SI0
4922
9274
616
91.2
globlastp

02070M_P1

LAB769_H3
switchgrass|12v1|DN148033_—
4923
9275
616
89.5
globlastp

P1

LAB637
barley|12v1|BG299942_P1
4924
496
617
89.4
globlastp

LAB770_H8
sorghum|12v1|SB12V2PRD0
4925
9276
617
84.6
globlastp

19448_P1

LAB770_H9
switchgrass|12v1|PV12v1PR
4926
9277
617
81.4
globlastp

D049054_P1

LAB771_H1
sugarcane|10v1|CA081882
4927
9278
618
93.6
globlastp

LAB771_H2
maize|10v1|AI941802_P1
4928
9279
618
87.4
globlastp

LAB771_H3
switchgrass|12v1|FL692950_—
4929
9280
618
80.1
globlastp

P1

LAB772_H1
maize|10v1|BE519282_P1
4930
9281
619
85.2
globlastp

LAB772_H2
foxtail_millet|11v3|PHY7SI0
4931
9282
619
81.3
globlastp

30470M_P1

LAB772_H3
switchgrass|12v1|FL754977_—
4932
9283
619
80.7
globlastp

P1

LAB772_H4
switchgrass|12v1|FL709979_—
4933
9284
619
80.4
globlastp

P1

LAB774_H1
sugarcane|10v1|CA110976
4934
9285
621
85.9
globlastp

LAB774_H2
maize|10v1|BG360671_P1
4935
9286
621
84.2
globlastp

LAB774_H3
maize|10v1|AW433466_P1
4936
9287
621
82.6
globlastp

LAB774_H4
foxtail_millet|11v3|PHY7SI0
4937
9288
621
82
globlastp

26915M_P1

LAB774_H5
cenchrus|gb166|EB657496_P
4938
9289
621
80.1
globlastp

1

LAB776_H1
maize|10v1|AI820267_T1
4939
9290
623
97.4
glotblastn

LAB776_H2
maize|10v1|AI964431_P1
4940
9291
623
96.8
globlastp

LAB776_H8
switchgrass|12v1|FL735004_—
4941
9292
623
90.3
globlastp

P1

LAB776_H3
switchgrass|gb167|FE607266
4942
9293
623
90.3
globlastp

LAB776_H4
foxtail_millet|11v3|PHY7SI0
4943
9294
623
89
globlastp

11233M_P1

LAB776_H3
switchgrass|12v1|FE607266_—
4944
9295
623
88.3
globlastp

P1

LAB776_H5
millet|10v1|EVO454PM1006
4945
9296
623
88.3
globlastp

62_P1

LAB776_H6
cynodon|10v1|ES306077_T1
4946
9297
623
83.44
glotblastn

LAB776_H7
sugarcane|10v1|CF572448
4947
9298
623
83.12
glotblastn

LAB777_H1
sugarcane|10v1|CA073655
4948
9299
624
97.9
globlastp

LAB777_H2
cenchrus|gb166|EB652913_P
4949
9300
624
95
globlastp

1

LAB777_H3
sugarcane|10v1|BQ529871
4950
9301
624
95
globlastp

LAB777_H4
switchgrass|12v1|DN150112_—
4951
9302
624
95
globlastp

P1

LAB777_H4
switchgrass|gb167|FL738222
4952
9302
624
95
globlastp

LAB777_H5
foxtail_millet|11v3|PHY7SI0
4953
9303
624
94.3
globlastp

07467M_P1

LAB777_H6
millet|10v1|EVO454PM4822
4954
9304
624
92.9
globlastp

04_P1

LAB777_H7
switchgrass|gb167|DN150112
4955
9305
624
92.9
globlastp

LAB777_H8
maize|10v1|BE224777_P1
4956
9306
624
90.8
globlastp

LAB777_H9
maize|10v1|BE512607_P1
4957
9306
624
90.8
globlastp

LAB777_H10
rice|11v1|OSU19030
4958
9307
624
90.8
globlastp

LAB777_H11
lolium|10v1|AU248896_P1
4959
9308
624
81.6
globlastp

LAB778_H1
sugarcane|10v1|CA067949
4960
9309
625
99.2
globlastp

LAB778_H2
foxtail_millet|11v3|PHY7SI0
4961
9310
625
98.9
globlastp

10401M_P1

LAB778_H3
maize|10v1|AW067269_P1
4962
9311
625
98.9
globlastp

LAB778_H5
switchgrass|12v1|FE615622_—
4963
9312
625
98.6
globlastp

P1

LAB778_H4
maize|10v1|AI622258_P1
4964
9313
625
98.4
globlastp

LAB778_H5
switchgrass|gb167|FE615622
4965
9314
625
98.4
globlastp

LAB778_H6
cynodon|10v1|ES294272_T1
4966
9315
625
97.83
glotblastn

LAB778_H7
rice|11v1|BM421296
4967
9316
625
96.7
globlastp

LAB778_H8
brachypodium|12v1|BRADI5
4968
9317
625
96.2
globlastp

G12000_P1

LAB778_H14
wheat|12v3|BM135910_P1
4969
9318
625
95.4
globlastp

6

LAB778_H9
pseudoroegneria|gb167|FF347
4970
9319
625
95.4
globlastp

577

LAB778_H10
rye|12v1|DRR001012.144060
4971
9318
625
95.4
globlastp

LAB778_H11
wheat|10v2|BF293546
4972
9318
625
95.4
globlastp

LAB778_H12
barley|10v2|BF623086
4973
9320
625
95.1
globlastp

LAB778_H11
wheat|12v3|CD925906_P1
4974
9321
625
94.9
globlastp

LAB778_H14
banana|12v1|MAGEN201201
4975
9322
625
88.6
globlastp

7
0475_P1

LAB778_H14
aquilegia|10v2|DR925484_P1
4976
9323
625
87.6
globlastp

8

LAB778_H13
aquilegia|10v1|DR925484
4977
9323
625
87.6
globlastp

LAB778_H14
oil_palm|11v1|ES414401_P1
4978
9324
625
87.5
globlastp

LAB778_H15
grape|11v1|GSVIVT0100485
4979
9325
625
87.1
globlastp

9001_P1

LAB778_H16
oil_palm|11v1|SRR190698.11
4980
9326
625
87
globlastp

166_P1

LAB778_H17
cassava|09v1|CK644949_P1
4981
9327
625
86.8
globlastp

LAB778_H18
castorbean|12v1|EG657108_P
4982
9328
625
86.8
globlastp

1

LAB778_H19
amborella|12v3|FD439662_P
4983
9329
625
86.5
globlastp

1

LAB778_H20
papaya|gb165|EX253111_P1
4984
9330
625
86.4
globlastp

LAB778_H21
gossypium_raimondii|12v1|D
4985
9331
625
86.3
globlastp

T557358_P1

LAB778_H22
chelidonium|11v1|SRR08475
4986
9332
625
86.29
glotblastn

2X110977_T1

LAB778_H23
apple|11v1|DT042056_P1
4987
9333
625
86
globlastp

LAB778_H24
clementine|11v1|CX069420_—
4988
9334
625
86
globlastp

P1

LAB778_H25
cotton|11v1|CO094851_P1
4989
9335
625
86
globlastp

LAB778_H26
orange|11v1|CX069420_P1
4990
9334
625
86
globlastp

LAB778_H27
pigeonpea|11v1|SRR054580X
4991
9336
625
86
globlastp

203000_P1

LAB778_H28
soybean|11v1|GLYMA18G02
4992
9337
625
86
globlastp

020

LAB778_H28
soybean|12v1|GLYMA18G02
4993
9337
625
86
globlastp

020_P1

LAB778_H29
cacao|10v1|EH057818_P1
4994
9338
625
85.9
globlastp

LAB778_H30
oil_palm|11v1|SRR190698.20
4995
9339
625
85.9
globlastp

4428_P1

LAB778_H31
thellungiella_halophilum|11v
4996
9340
625
85.9
globlastp

1|EHJGI11028729

LAB778_H14
prunus_mume|13v1|CV04992
4997
9341
625
85.7
globlastp

9
4_P1

LAB778_H32
apple|11v1|CN928566_P1
4998
9342
625
85.7
globlastp

LAB778_H33
cotton|11v1|DT557358_P1
4999
9343
625
85.7
globlastp

LAB778_H34
prunus|10v1|CN928566
5000
9344
625
85.7
globlastp

LAB778_H35
soybean|11v1|GLYMA05G31
5001
9345
625
85.7
globlastp

170

LAB778_H35
soybean|12v1|GLYMA05G31
5002
9345
625
85.7
globlastp

170_P1

LAB778_H36
soybean|11v1|GLYMA11G38
5003
9346
625
85.7
globlastp

090

LAB778_H36
soybean|12v1|GLYMA11G38
5004
9346
625
85.7
globlastp

090_P1

LAB778_H37
amsonia|11v1|SRR098688X1
5005
9347
625
85.64
glotblastn

02716_T1

LAB778_H15
banana|12v1|MAGEN201202
5006
9348
625
85.6
globlastp

0
4778_P1

LAB778_H38
potato|10v1|BG592780_P1
5007
9349
625
85.6
globlastp

LAB778_H39
solanum_phureja|09v1|SPHB
5008
9349
625
85.6
globlastp

G629261

LAB778_H40
cotton|11v1|DW485628_P1
5009
9350
625
85.4
globlastp

LAB778_H41
gossypium_raimondii|12v1|D
5010
9350
625
85.4
globlastp

R453115_P1

LAB778_H42
lotus|09v1|GO031508_P1
5011
9351
625
85.4
globlastp

LAB778_H43
soybean|11v1|GLYMA08G14
5012
9352
625
85.4
globlastp

360

LAB778_H43
soybean|12v1|GLYMA08G14
5013
9352
625
85.4
globlastp

360_P1

LAB778_H44
strawberry|11v1|SRR034865S
5014
9353
625
85.4
globlastp

0053110

LAB778_H45
chestnut|gb170|SRR006295S0
5015
9354
625
85.2
globlastp

011045_P1

LAB778_H46
euphorbia|11v1|DV153284X
5016
9355
625
85.2
globlastp

X1_P1

LAB778_H47
oak|10v1|DB997278_P1
5017
9356
625
85.2
globlastp

LAB778_H48
poplar|10v1|BI130238
5018
9357
625
85.2
globlastp

LAB778_H48
poplar|13v1|BI130238_P1
5019
9357
625
85.2
globlastp

LAB778_H49
solanum_phureja|09v1|SPHB
5020
9358
625
85.2
globlastp

G097630

LAB778_H50
trigonella|11v1|SRR066194X
5021
9359
625
85.2
globlastp

205048

LAB778_H51
watermelon|11v1|AM724931
5022
9360
625
85.2
globlastp

LAB778_H57
cowpea|12v1|FF393869_P1
5023
9361
625
85.2
globlastp

LAB778_H52
cotton|11v1|DR452607_P1
5024
9362
625
85.1
globlastp

LAB778_H53
gossypium_raimondii|12v1|D
5025
9363
625
85.1
globlastp

R452607_P1

LAB778_H54
ambrosia|11v1|SRR346935.1
5026
9364
625
84.9
globlastp

23282_P1

LAB778_H55
ambrosia|11v1|SRR346935.2
5027
9365
625
84.9
globlastp

08678_P1

LAB778_H56
arabidopsis|10v1|AT2G22310
5028
9366
625
84.9
globlastp

_P1

LAB778_H57
cowpea|gb166|FF393869
5029
9367
625
84.9
globlastp

LAB778_H58
peanut|10v1|CD037908_P1
5030
9368
625
84.9
globlastp

LAB778_H59
pigeonpea|11v1|SRR054580X
5031
9369
625
84.9
globlastp

102551_P1

LAB778_H60
poplar|10v1|BU890899
5032
9370
625
84.9
globlastp

LAB778_H60
poplar|13v1|BU890899_P1
5033
9370
625
84.9
globlastp

LAB778_H15
nicotiana_benthamiana|12v1|
5034
9371
625
84.8
globlastp

1
BP749169_P1

LAB778_H15
bean|12v2|FG231136_P1
5035
9372
625
84.6
globlastp

2

LAB778_H61
bean|12v1|FG231136
5036
9372
625
84.6
globlastp

LAB778_H62
cannabis|12v1|JK494861_P1
5037
9373
625
84.6
globlastp

LAB778_H63
cirsium|11v1|SRR346952.104
5038
9374
625
84.6
globlastp

5820XX1_P1

LAB778_H64
cucumber|09v1|DV631552_P
5039
9375
625
84.6
globlastp

1

LAB778_H65
sunflower|12v1|BQ966718
5040
9376
625
84.6
globlastp

LAB778_H66
sunflower|12v1|EE617132
5041
9377
625
84.6
globlastp

LAB778_H67
ambrosia|11v1|SRR346935.2
5042
9378
625
84.55
glotblastn

44240_T1

LAB778_H15
nicotiana_benthamiana|12v1|
5043
9379
625
84.4
globlastp

3
EH624032_P1

LAB778_H68
chickpea|11v1|GR408350
5044
9380
625
84.4
globlastp

LAB778_H69
cirsium|11v1|SRR346952.100
5045
9381
625
84.4
globlastp

0105_P1

LAB778_H70
tomato|11v1|BG097630
5046
9382
625
84.4
globlastp

LAB778_H71
tomato|11v1|BG629261
5047
9383
625
84.3
globlastp

LAB778_H15
blueberry|12v1|SRR353282X
5048
9384
625
84.28
glotblastn

4
35736D1_T1

LAB778_H72
ambrosia|11v1|SRR346935.1
5049
9385
625
84.28
glotblastn

91390_T1

LAB778_H73
ambrosia|11v1|SRR346935.2
5050
9386
625
84.28
glotblastn

12665_T1

LAB778_H74
flaveria|11v1|SRR149229.219
5051
9387
625
84.28
glotblastn

118_T1

LAB778_H75
cirsium|11v1|SRR346952.100
5052
9388
625
84.1
globlastp

3793_P1

LAB778_H76
flaveria|11v1|SRR149229.106
5053
9389
625
84.1
globlastp

79_P1

LAB778_H77
thellungiella_parvulum|11v1|
5054
9390
625
84.1
globlastp

DN774189

LAB778_H78
ambrosia|11v1|SRR346943.1
5055
9391
625
84.01
glotblastn

11704_T1

LAB778_H79
humulus|11v1|SRR098683X7
5056
9392
625
84
globlastp

1787_P1

LAB778_H80
canola|11v1|EE440813_P1
5057
9393
625
83.9
globlastp

LAB778_H81
centaurea|gb166|EH713812_P
5058
9394
625
83.8
globlastp

1

LAB778_H82
oil_palm|11v1|EY412217_P1
5059
9395
625
83.7
globlastp

LAB778_H15
bean|12v2|EC911834_P1
5060
9396
625
83.6
globlastp

5

LAB778_H15
lettuce|12v1|DW109814_P1
5061
9397
625
83.6
globlastp

6

LAB778_H83
arabidopsis_lyrata|09v1|JGIA
5062
9398
625
83.6
globlastp

L024106_P1

LAB778_H84
arabidopsis|10v1|AT4G39910
5063
9399
625
83.6
globlastp

_P1

LAB778_H86
thellungiella_halophilum|11v
5064
9400
625
83.6
globlastp

1|DN774189

LAB778_H87
cirsium|11v1|SRR346952.106
5065
9401
625
83.56
glotblastn

910_T1

LAB778_H15
zostera|12v1|SRR057351X10
5066
9402
625
83.33
glotblastn

7
4064D1_T1

LAB778_H88
artemisia|10v1|EY048740_P1
5067
9403
625
83.3
globlastp

LAB778_H89
eucalyptus|11v2|CU396941_P
5068
9404
625
83.3
globlastp

1

LAB778_H90
flaveria|11v1|SRR149229.120
5069
9405
625
83.3
globlastp

455_P1

LAB778_H91
b_rapa|11v1|CB686216_P1
5070
9406
625
83.2
globlastp

LAB778_H92
canola|11v1|DY000736_P1
5071
9406
625
83.2
globlastp

LAB778_H93
canola|11v1|EG020175_P1
5072
9406
625
83.2
globlastp

LAB778_H94
flaveria|11v1|SRR149232.110
5073
9407
625
83.15
glotblastn

533_T1

LAB778_H95
b_rapa|11v1|CD815757_P1
5074
9408
625
83.1
globlastp

LAB778_H15
sesame|12v1|BU668130_P1
5075
9409
625
83
globlastp

8

LAB778_H96
arnica|11v1|SRR099034X117
5076
9410
625
83
globlastp

913_P1

LAB778_H97
oat|11v1|CN820234_P1
5077
9411
625
82.9
globlastp

LAB778_H98
silene|11v1|SRR096785X127
5078
9412
625
82.88
glotblastn

418

LAB778_H99
lettuce|10v1|DW067326
5079
9413
625
82.8
globlastp

LAB778_H10
poppy|11v1|SRR030259.1214
5080
9414
625
82.6
globlastp

0
84_P1

LAB778_H10
triphysaria|10v1|DR171314
5081
9415
625
82.4
globlastp

1

LAB778_H10
radish|gb164|EW725241
5082
9416
625
82.35
glotblastn

2

LAB778_H10
utricularia|11v1|SRR094438.1
5083
9417
625
82.2
globlastp

3
03840

LAB778_H10
brachypodium|12v1|BRADI1
5084
9418
625
82.1
globlastp

4
G71830_P1

LAB778_H10
rice|11v1|BI813450
5085
9419
625
82.1
globlastp

5

LAB778_H10
maritime_pine|10v1|CT57718
5086
9420
625
82
globlastp

6
3_P1

LAB778_H10
lettuce|10v1|DW046238
5087
9421
625
81.8
globlastp

7

LAB778_H10
monkeyflower|10v1|GO98713
5088
9422
625
81.77
glotblastn

8
0

LAB778_H10
monkeyflower|12v1|SRR0372
5089
9423
625
81.77
glotblastn

8
27.103136_T1

LAB778_H10
pine|10v2|CF391012_P1
5090
9424
625
81.7
globlastp

9

LAB778_H11
spruce|11v1|ES877270
5091
9425
625
81.7
globlastp

0

LAB778_H11
b_rapa|11v1|SRR019556.174
5092
9426
625
81.6
globlastp

1
88_P1

LAB778_H11
sugarcane|10v1|BQ478957
5093
9427
625
81.6
globlastp

2

LAB778_H11
oat|11v1|CN820191_T1
5094
9428
625
81.52
glotblastn

3

LAB778_H11
foxtail_millet|11v3|PHY7SI0
5095
9429
625
81.5
globlastp

4
36284M_P1

LAB778_H11
maize|10v1|AI783101_P1
5096
9430
625
81.5
globlastp

5

LAB778_H11
cassava|09v1|DV457465_P1
5097
9431
625
81.4
globlastp

6

LAB778_H15
banana|12v1|MAGEN201200
5098
9432
625
81.3
globlastp

9
4141_P1

LAB778_H11
ceratodon|10v1|SRR074890S
5099
9433
625
81.3
globlastp

7
0131228_P1

LAB778_H11
barley|10v2|AV832449
5100
9434
625
81.2
globlastp

8

LAB778_H11
barley|12v1|AV832449_P1
5101
9434
625
81.2
globlastp

8

LAB778_H11
physcomitrella|10v1|BJ17350
5102
9435
625
81.2
globlastp

9
3_P1

LAB778_H12
spruce|11v1|ES664529
5103
9436
625
81.2
globlastp

0

LAB778_H12
cichorium|gb171|EH696895_—
5104
9437
625
81.13
glotblastn

1
T1

LAB778_H16
wheat|12v3|CA616068_P1
5105
9438
625
81
globlastp

0

LAB778_H12
physcomitrella|10v1|BJ15958
5106
9439
625
81
globlastp

2
8_P1

LAB778_H12
wheat|10v2|CA498148
5107
9440
625
81
globlastp

3

LAB778_H12
wheat|12v3|CA498148_P1
5108
9440
625
81
globlastp

3

LAB778_H12
brachypodium|12v1|BRADI3
5109
9441
625
80.98
glotblastn

4
G46610_T1

LAB778_H12
millet|10v1|EVO454PM0182
5110
9442
625
80.98
glotblastn

5
50_T1

LAB778_H12
canola|11v1|ES979784_T1
5111
9443
625
80.91
glotblastn

6

LAB778_H12
pseudotsuga|10v1|SRR06511
5112
9444
625
80.9
globlastp

7
9S0049871

LAB778_H12
amorphophallus|11v2|SRR08
5113
9445
625
80.76
glotblastn

8
9351X312027XX1_T1

LAB778_H12
silene|11v1|SRR096785X148
5114
9446
625
80.76
glotblastn

9
997

LAB778_H13
orobanche|10v1|SRR023189S
5115
9447
625
80.71
glotblastn

0
0000975_T1

LAB778_H13
millet|10v1|EVO454PM0188
5116
9448
625
80.7
globlastp

1
21_P1

LAB778_H13
rye|12v1|BE494881
5117
9449
625
80.7
globlastp

2

LAB778_H13
switchgrass|gb167|FE601077
5118
9450
625
80.7
globlastp

3

LAB778_H13
pine|10v2|BX253084_P1
5119
9451
625
80.6
globlastp

4

LAB778_H13
pseudotsuga|10v1|SRR06511
5120
9452
625
80.6
globlastp

5
9S0041451

LAB778_H13
sorghum|12v1|SB01G044530
5121
9453
625
80.5
globlastp

6

LAB778_H13
radish|gb164|EX905823
5122
9454
625
80.43
glotblastn

7

LAB778_H16
wheat|12v3|CA604484_P1
5123
9455
625
80.4
globlastp

1

LAB778_H13
cephalotaxus|11v1|SRR06439
5124
9456
625
80.38
glotblastn

8
5X140948_T1

LAB778_H13
phyla|11v2|SRR099035X135
5125
9457
625
80.2
globlastp

9
649_P1

LAB778_H14
valeriana|11v1|SRR099039X1
5126
9458
625
80.2
globlastp

0
04838

LAB778_H14
artemisia|10v1|SRR019254S0
5127
9459
625
80.16
glotblastn

1
050962_T1

LAB778_H14
physcomitrella|10v1|BY9466
5128
9460
625
80.16
glotblastn

2
60_T1

LAB778_H14
abies|11v2|SRR098676X1018
5129
9461
625
80.1
globlastp

3
86_P1

LAB778_H14
vinca|11v1|SRR098690X1392
5130
9462
625
80.1
globlastp

4
84

LAB778_H14
triphysaria|10v1|BM356629
5131
9463
625
80
globlastp

5

LAB779_H1
maize|10v1|AI861392_P1
5132
9464
626
97.9
globlastp

LAB779_H2
switchgrass|12v1|FE621281_—
5133
9465
626
96.1
globlastp

P1

LAB779_H2
switchgrass|gb167|FE621280
5134
9466
626
96.1
globlastp

LAB779_H3
foxtail_millet|11v3|EC612909
5135
9467
626
95.3
globlastp

_P1

LAB779_H4
rice|11v1|BE040494
5136
9468
626
93.8
globlastp

LAB779_H5
barley|10v2|BF257464
5137
9469
626
90.4
globlastp

LAB779_H5
barley|12v1|BF257464_P1
5138
9469
626
90.4
globlastp

LAB779_H6
brachypodium|12v1|BRADI5
5139
9470
626
90.2
globlastp

G12110_P1

LAB779_H7
rye|12v1|DRR001012.173397
5140
9471
626
89.9
globlastp

LAB779_H8
wheat|10v2|BE404593
5141
9472
626
89.9
globlastp

LAB779_H8
wheat|12v3|BE404593_P1
5142
9472
626
89.9
globlastp

LAB779_H9
fescue|gb161|DT688231_P1
5143
9473
626
88.9
globlastp

LAB779_H10
aristolochia|10v1|FD760907_—
5144
9474
626
83.4
globlastp

P1

LAB779_H11
castorbean|11v1|XM_002513
5145
9475
626
82.9
globlastp

850

LAB779_H11
castorbean|12v1|XM_002513
5146
9475
626
82.9
globlastp

850_P1

LAB779_H12
poplar|10v1|BU826815
5147
9476
626
82.9
globlastp

LAB779_H12
poplar|13v1|BU826815_P1
5148
9477
626
82.6
globlastp

LAB779_H13
cacao|10v1|CF973691_P1
5149
9478
626
82.4
globlastp

LAB779_H14
nasturtium|11v1|GH169581_P
5150
9479
626
82.4
globlastp

1

LAB779_H15
pigeonpea|11v1|GW348250_—
5151
9480
626
82.4
globlastp

P1

LAB779_H16
soybean|11v1|GLYMA14G34
5152
9481
626
82.4
globlastp

600

LAB779_H16
soybean|12v1|GLYMA14G34
5153
9481
626
82.4
globlastp

600_P1

LAB779_H44
bean|12v2|CA847635_P1
5154
9482
626
82.1
globlastp

LAB779_H17
bean|12v1|CA847635
5155
9482
626
82.1
globlastp

LAB779_H18
beech|11v1|SRR006293.2506
5156
9483
626
82.1
globlastp

7_P1

LAB779_H19
tabernaemontana|11v1|SRR09
5157
9484
626
82.1
globlastp

8689X114013

LAB779_H45
aquilegia|10v2|DR917100_P1
5158
9485
626
81.9
globlastp

LAB779_H20
aquilegia|10v1|DR917100
5159
9485
626
81.9
globlastp

LAB779_H21
cannabis|12v1|GR222042_P1
5160
9486
626
81.9
globlastp

LAB779_H22
grape|11v1|GSVIVT0100900
5161
9487
626
81.9
globlastp

9001_P1

LAB779_H46
prunus_mume|13v1|CB81982
5162
9488
626
81.6
globlastp

9_P1

LAB779_H47
switchgrass|12v1|FE621280_—
5163
9489
626
81.6
globlastp

P1

LAB779_H23
euonymus|11v1|SRR070038X
5164
9490
626
81.3
globlastp

15779_P1

LAB779_H24
papaya|gb165|EX245984_P1
5165
9491
626
81.3
globlastp

LAB779_H25
prunus|10v1|CB819829
5166
9492
626
81.3
globlastp

LAB779_H48
banana|12v1|BBS173T3_P1
5167
9493
626
81.1
globlastp

LAB779_H26
apple|11v1|CN494364_P1
5168
9494
626
81.1
globlastp

LAB779_H27
cotton|11v1|AI728059_P1
5169
9495
626
81.1
globlastp

LAB779_H28
gossypium_raimondii|12v1|AI
5170
9496
626
81.1
globlastp

728059_P1

LAB779_H29
humulus|11v1|GD248389_P1
5171
9497
626
81.1
globlastp

LAB779_H30
soybean|11v1|GLYMA13G01
5172
9498
626
81.1
globlastp

920

LAB779_H30
soybean|12v1|GLYMA13G01
5173
9498
626
81.1
globlastp

920_P1

LAB779_H31
amsonia|11v1|SRR098688X1
5174
9499
626
81.09
glotblastn

70183_T1

LAB779_H32
flax|11v1|EB711727_T1
5175
9500
626
81.09
glotblastn

LAB779_H33
strawberry|11v1|SRR034860S
5176
9501
626
80.83
glotblastn

0006032

LAB779_H34
cotton|11v1|CO085575_P1
5177
9502
626
80.8
globlastp

LAB779_H35
oak|10v1|DN950218_P1
5178
9503
626
80.8
globlastp

LAB779_H36
poplar|10v1|BU823969
5179
9504
626
80.8
globlastp

LAB779_H36
poplar|13v1|BU823969_P1
5180
9504
626
80.8
globlastp

LAB779_H37
peanut|10v1|ES719251_P1
5181
9505
626
80.6
globlastp

LAB779_H38
rhizophora|10v1|SRR005792S
5182
9506
626
80.6
globlastp

0003758

LAB779_H49
amborella|12v3|FD439477_P
5183
9507
626
80.3
globlastp

1

LAB779_H50
banana|12v1|FL665645_P1
5184
9508
626
80.3
globlastp

LAB779_H51
olea|13v1|SRR014463X48161
5185
9509
626
80.3
globlastp

D1_P1

LAB779_H39
monkeyflower|12v1|DV20764
5186
9510
626
80.3
globlastp

9_P1

LAB779_H43
chickpea|13v2|GR399123_P1
5187
9511
626
80.3
globlastp

LAB779_H40
b_rapa|11v1|CX190909_P1
5188
9512
626
80.1
globlastp

LAB779_H41
orange|11v1|CF418185_P1
5189
9513
626
80.1
globlastp

LAB779_H42
rose|12v1|EC587962
5190
9514
626
80.1
globlastp

LAB779_H43
chickpea|11v1|GR399123
5191
9515
626
80.05
glotblastn

LAB780_H1
maize|10v1|AI600277_P1
5192
9516
627
87
globlastp

LAB780_H3
switchgrass|12v1|HO265040_—
5193
9517
627
84.6
globlastp

P1

LAB780_H4
switchgrass|12v1|FL887464_—
5194
9518
627
83.7
globlastp

P1

LAB780_H2
foxtail_millet|11v3|PHY7SI0
5195
9519
627
83.4
globlastp

10679M_P1

LAB781_H1
sugarcane|10v1|CA128240X
5196
9520
628
87.3
globlastp

X1

LAB781_H4
switchgrass|12v1|SRR187765
5197
9521
628
80.95
glotblastn

.495706_T1

LAB781_H2
maize|10v1|SRR014551S037
5198
9522
628
80.95
glotblastn

7837_T1

LAB781_H3
switchgrass|12v1|FL777489_—
5199
9523
628
80.95
glotblastn

T1

LAB781_H3
switchgrass|gb167|FL777489
5200
9524
628
80.95
glotblastn

LAB782_H1
sugarcane|10v1|CA066605
5201
9525
629
94.1
globlastp

LAB782_H2
maize|10v1|BM078700_P1
5202
9526
629
88.5
globlastp

LAB782_H3
foxtail_millet|11v3|PHY7SI0
5203
9527
629
86.4
globlastp

09956M_P1

LAB782_H4
millet|10v1|EVO454PM0124
5204
9528
629
83.4
globlastp

28_P1

LAB782_H5
switchgrass|gb167|FE623288
5205
9529
629
81.79
glotblastn

LAB783_H1
foxtail_millet|11v3|PHY7SI0
5206
9530
630
92.1
globlastp

10613M_P1

LAB783_H2
maize|10v1|CD941129_P1
5207
9531
630
91.6
globlastp

LAB783_H6
switchgrass|12v1|SRR187770
5208
9532
630
87.2
globlastp

.838615_P1

LAB783_H7
switchgrass|12v1|SRR187766
5209
9533
630
87.1
globlastp

.338407_P1

LAB783_H3
wheat|10v2|BF484446
5210
9534
630
81.3
globlastp

LAB783_H4
rice|11v1|BI305734
5211
9535
630
80.5
globlastp

LAB783_H5
rice|11v1|AU031195
5212
9536
630
80.27
glotblastn

LAB783_H8
wheat|12v3|BQ901541_P1
5213
9537
630
80
globlastp

LAB784_H1
maize|10v1|CO442798_P1
5214
9538
631
90.9
globlastp

LAB784_H2
foxtail_millet|11v3|PHY7SI0
5215
9539
631
88.6
globlastp

09889M_P1

LAB784_H3
switchgrass|12v1|DN142888_—
5216
9540
631
86.3
globlastp

P1

LAB784_H3
switchgrass|gb167|DN142888
5217
9541
631
85.9
globlastp

LAB784_H4
switchgrass|gb167|DN142031
5218
9542
631
85.3
globlastp

LAB784_H4
switchgrass|12v1|DN142031_—
5219
9543
631
85.1
globlastp

P1

LAB784_H11
switchgrass|12v1|SRR187767
5220
9544
631
83.5
globlastp

.875872_P1

LAB784_H5
foxtail_millet|11v3|PHY7SI0
5221
9545
631
83.2
globlastp

12090M_P1

LAB784_H12
wheat|12v3|BE497227_T1
5222
9546
631
82.91
glotblastn

LAB784_H6
maize|10v1|DT944637_P1
5223
9547
631
82.7
globlastp

LAB784_H7
rye|12v1|DRR001012.122081
5224
9548
631
82.7
globlastp

LAB784_H13
barley|12v1|BU972778_P1
5225
9549
631
82.4
globlastp

LAB784_H8
foxtail_millet|11v3|PHY7SI0
5226
9550
631
82.1
globlastp

09893M_P1

LAB784_H9
sorghum|12v1|SB06G025990
5227
9551
631
81.8
globlastp

LAB784_H10
brachypodium|12v1|BRADI5
5228
9552
631
81.29
glotblastn

G18920_T1

LAB785_H1
maize|10v1|AI943649_P1
5229
9553
632
93.9
globlastp

LAB785_H2
maize|10v1|CO522204_P1
5230
9554
632
92.7
globlastp

LAB785_H3
foxtail_millet|11v3|PHY7SI0
5231
9555
632
89.8
globlastp

09822M_P1

LAB785_H4
switchgrass|gb167|DN145209
5232
9556
632
89.5
globlastp

LAB785_H5
maize|10v1|DT652662_T1
5233
9557
632
88.91
glotblastn

LAB785_H6
maize|10v1|AW447873_T1
5234
9558
632
88.34
glotblastn

LAB785_H7
rice|11v1|CA762994
5235
9559
632
87.5
globlastp

LAB785_H9
wheat|12v3|BE415088XXl_P
5236
9560
632
86
globlastp

1

LAB785_H10
barley|12v1|BU996159_P1
5237
9561
632
85.5
globlastp

LAB785_H8
brachypodium|12v1|BRADI5
5238
9562
632
84.6
globlastp

G19170_P1

LAB786_H1
maize|10v1|CD940584_P1
5239
9563
633
87.8
globlastp

LAB787_H1
maize|10v1|CO517647_P1
5240
9564
634
89
globlastp

LAB787_H2
switchgrass|12v1|FL887410_—
5241
9565
634
85.8
globlastp

P1

LAB787_H3
switchgrass|12v1|FL887409_—
5242
9566
634
85.5
globlastp

P1

LAB787_H4
foxtail_millet|11v3|PHY7SI0
5243
9567
634
85
globlastp

14111M_P1

LAB787_H5
fescue|gb161|DT679567_T1
5244
9568
634
80.19
glotblastn

LAB787_H6
wheat|12v3|BQ904010_T1
5245
9569
634
80.19
glotblastn

LAB787_H7
brachypodium|12v1|BRADI3
5246
9570
634
80.1
globlastp

G38777_P1

LAB788_H1
sorghum|12v1|SB12V2PRD0
5247
—
635
98.4
glotblastn

17103

LAB788_H2
sugarcane|10v1|CA260219
5248
9571
635
97.6
globlastp

LAB788_H3
millet|10v1|EVO454PM1313
5249
9572
635
96.8
globlastp

84_P1

LAB788_H4
wheat|10v2|CA485481
5250
9573
635
96
globlastp

LAB788_H5
foxtail_millet|11v3|PHY7SI0
5251
9574
635
94.4
globlastp

38047M_P1

LAB788_H6
switchgrass|gb167|FL786981
5252
9575
635
94.4
globlastp

LAB788_H7
switchgrass|gb167|GD025782
5253
9576
635
93.5
globlastp

LAB788_H6, L
switchgrass|12v1|FE618625_—
5254
9576
635
93.5
globlastp

AB788_H7
P1

LAB788_H8
maize|10v1|CD943276_P1
5255
9577
635
92.8
globlastp

LAB788_H23
switchgrass|12v1|SRR187766
5256
9578
635
92.74
glotblastn

.237753_T1

LAB788_H9
maize|10v1|BG517888_P1
5257
9579
635
92.7
globlastp

LAB788_H10
maize|10v1|BG354557_P1
5258
9580
635
92
globlastp

LAB788_H11
maize|10v1|EU972756_P1
5259
9581
635
91.2
globlastp

LAB788_H12
cynodon|10v1|ES292289_P1
5260
9582
635
91.1
globlastp

LAB788_H13
sorghum|12v1|SB01G038765
5261
9583
635
90.32
glotblastn

LAB788_H14
wheat|10v2|AL825356
5262
9584
635
85.6
globlastp

LAB788_H14
wheat|12v3|AL825356_P1
5263
9584
635
85.6
globlastp

LAB788_H15
rice|11v1|CA761785
5264
9585
635
84.1
globlastp

LAB788_H16
wheat|10v2|CA485751
5265
9585
635
84.1
globlastp

LAB788_H16
wheat|12v3|CA485751_P1
5266
9585
635
84.1
globlastp

LAB788_H17
brachypodium|12v1|BRADI1
5267
9586
635
84
globlastp

G65850_P1

LAB788_H18
rye|12v1|DRR001012.11660
5268
9587
635
84
globlastp

LAB788_H19
rye|12v1|DRR001012.217026
5269
9587
635
84
globlastp

LAB788_H20
barley|10v2|AV923028
5270
9588
635
83.2
globlastp

LAB788_H21
wheat|10v2|CA650666
5271
9589
635
83.2
globlastp

LAB788_H22
oat|11v1|GO598161_P1
5272
9590
635
82.4
globlastp

LAB791_H1
sugarcane|10v1|BQ529695
5273
9591
638
99.5
globlastp

LAB791_H2
maize|10v1|AI783079_P1
5274
9592
638
97.9
globlastp

LAB791_H3
foxtail_millet|11v3|PHY7SI0
5275
9593
638
97.4
globlastp

23315M_P1

LAB791_H4
sugarcane|10v1|CA081540
5276
9594
638
97.4
globlastp

LAB791_H24
switchgrass|12v1|DN143962_—
5277
9595
638
96.9
globlastp

P1

LAB791_H5
millet|10v1|EVO454PM0059
5278
9596
638
96.9
globlastp

58_P1

LAB791_H6
switchgrass|gb167|DN143962
5279
9595
638
96.9
globlastp

LAB791_H7
switchgrass|12v1|FE625453_—
5280
9597
638
96.4
globlastp

P1

LAB791_H7
switchgrass|gb167|FE625453
5281
9597
638
96.4
globlastp

LAB791_H8
maize|10v1|AW018263_P1
5282
9598
638
95.9
globlastp

LAB791_H25
wheat|12v3|BE445087_P1
5283
9599
638
93.9
globlastp

LAB791_H9
barley|10v2|BI950704
5284
9600
638
93.9
globlastp

LAB791_H9
barley|12v1|BI950704_P1
5285
9600
638
93.9
globlastp

LAB791_H10
brachypodium|12v1|BRADI4
5286
9601
638
93.9
globlastp

G06510_P1

LAB791_H11
rice|11v1|AU102059
5287
9602
638
93.8
globlastp

LAB791_H26
wheat|12v3|BE442756_P1
5288
9603
638
93.4
globlastp

LAB791_H27
wheat|12v3|SRR400826X517
5289
9603
638
93.4
globlastp

916D1_P1

LAB791_H12
pseudoroegneria|gb167|FF343
5290
9604
638
93.4
globlastp

861

LAB791_H13
wheat|10v2|BE442756
5291
9603
638
93.4
globlastp

LAB791_H13
wheat|12v3|BE499038_P1
5292
9605
638
93.4
globlastp

LAB791_H14
rye|12v1|BF146081
5293
9606
638
92.3
globlastp

LAB791_H15
lovegrass|gb167|EH184524_P
5294
9607
638
91.8
globlastp

1

LAB791_H16
fescue|gb161|DT702783_P1
5295
9608
638
90.8
globlastp

LAB791_H17
oat|11v1|CN814680_P1
5296
9609
638
90.8
globlastp

LAB791_H18
cynodon|10v1|BQ826443_P1
5297
9610
638
87.8
globlastp

LAB791_H28
banana|12v1|MAGEN201201
5298
9611
638
83.1
globlastp

6842_P1

LAB791_H29
banana|12v1|FF559352_P1
5299
9612
638
82.6
globlastp

LAB791_H30
banana|12v1|MAGEN201202
5300
9613
638
82.2
globlastp

6839_P1

LAB791_H19
phalaenopsis|11v1|SRR12577
5301
9614
638
81.12
glotblastn

1.1007715_T1

LAB791_H20
cacao|10v1|CU550744_T1
5302
9615
638
80.41
glotblastn

LAB791_H21
lotus|09v1|BW617043_T1
5303
9616
638
80.1
glotblastn

LAB791_H22
oil_palm|11v1|EL681923_T1
5304
9617
638
80.1
glotblastn

LAB791_H23
cowpea|12v1|FF384610_T1
5305
9618
638
80
glotblastn

LAB791_H23
cowpea|gb166|FF384610
5306
9618
638
80
glotblastn

LAB792_H1
sugarcane|10v1|CA096552
5307
9619
639
95.9
globlastp

LAB792_H18
switchgrass|12v1|FE600294_—
5308
9620
639
92.8
globlastp

P1

LAB792_H2
foxtail_millet|11v3|PHY7SI0
5309
9621
639
92.8
globlastp

23310M_P1

LAB792_H3
switchgrass|gb167|FE600294
5310
9620
639
92.8
globlastp

LAB792_H4
switchgrass|12v1|DN143401_—
5311
9622
639
91.8
globlastp

P1

LAB792_H4
switchgrass|gb167|DN143401
5312
9622
639
91.8
globlastp

LAB792_H5
millet|10v1|EVO454PM0416
5313
9623
639
89.7
globlastp

50_P1

LAB792_H6
cynodon|10v1|ES296132_P1
5314
9624
639
87.2
globlastp

LAB792_H7
lovegrass|gb167|EH184941_P
5315
9625
639
84.6
globlastp

1

LAB792_H8
brachypodium|12v1|BRADI2
5316
9626
639
84.2
globlastp

G37770_P1

LAB792_H9
fescue|gb161|DT680806_P1
5317
9627
639
83.1
globlastp

LAB792_H10
pseudoroegneria|gb167|FF346
5318
9628
639
82.1
globlastp

074

LAB792_H11
barley|10v2|BI949166
5319
9629
639
81.6
globlastp

LAB792_H11
barley|12v1|BI949166_P1
5320
9629
639
81.6
globlastp

LAB792_H12
leymus|gb166|EG393802_P1
5321
9630
639
81.1
globlastp

LAB792_H19
wheat|12v3|BE400950_P1
5322
9631
639
80.8
globlastp

LAB792_H13
rye|12v1|DRR001012.129875
5323
9632
639
80.7
globlastp

LAB792_H14
rye|12v1|DRR001012.219766
5324
9632
639
80.7
globlastp

LAB792_H15
oat|11v1|GR340758_P1
5325
9633
639
80.6
globlastp

LAB792_H16
rice|11v1|BI809637
5326
9634
639
80.5
globlastp

LAB792_H17
wheat|10v2|BE400950
5327
9635
639
80.3
globlastp

LAB792_H17
wheat|12v3|CA485207_P1
5328
9635
639
80.3
globlastp

LAB793_H1
sugarcane|10v1|CA083729
5329
9636
640
97.6
globlastp

LAB793_H2
maize|10v1|BG349275_P1
5330
9637
640
94.9
globlastp

LAB793_H3
foxtail_millet|11v3|PHY7SI0
5331
9638
640
93.9
globlastp

22841M_P1

LAB793_H4
millet|10v1|EVO454PM0059
5332
9639
640
93.5
globlastp

49_P1

LAB793_H5
switchgrass|gb167|FE629745
5333
9640
640
92.9
globlastp

LAB793_H6
switchgrass|12v1|FE623446_—
5334
9641
640
91.2
globlastp

P1

LAB793_H6
switchgrass|gb167|FE623446
5335
9641
640
91.2
globlastp

LAB793_H7
rice|11v1|BI811508
5336
9642
640
89.1
globlastp

LAB793_H8
brachypodium|12v1|BRADI2
5337
9643
640
86.7
globlastp

G37950_P1

LAB793_H16
wheat|12v3|BE213352_P1
5338
9644
640
85.4
globlastp

LAB793_H9
wheat|10v2|BE470964
5339
9645
640
85.4
globlastp

LAB793_H9
wheat|12v3|BE470964_P1
5340
9645
640
85.4
globlastp

LAB793_H10
rye|12v1|DRR001012.126664
5341
9646
640
84.7
globlastp

LAB793_H11
rye|12v1|DRR001012.149681
5342
9646
640
84.7
globlastp

LAB793_H12
rye|12v1|DRR001012.169047
5343
9646
640
84.7
globlastp

LAB793_H13
pseudoroegneria|gb167|FF353
5344
9647
640
84.4
globlastp

233

LAB793_H14
barley|10v2|BE420584XX1
5345
9648
640
84
globlastp

LAB793_H14
barley|12v1|BE420584_P1
5346
9648
640
84
globlastp

LAB793_H15
oat|11v1|GR344693_P1
5347
9649
640
81.3
globlastp

LAB794_H1
maize|10v1|AI372267_P1
5348
9650
641
87.7
globlastp

LAB794_H2
sorghum|12v1|SB09G004660
5349
9651
641
83
globlastp

LAB794_H3
maize|10v1|BG841443_P1
5350
9652
641
80.8
globlastp

LAB795_H1
maize|10v1|AI833936_P1
5351
9653
642
90.1
globlastp

LAB795_H2
foxtail_millet|11v3|PHY7SI0
5352
9654
642
88.9
globlastp

21709M_P1

LAB795_H3
maize|10v1|CB351667_P1
5353
9655
642
88.3
globlastp

LAB795_H5
switchgrass|12v1|FL750760_—
5354
9656
642
87.8
globlastp

P1

LAB795_H4
rice|11v1|AU085763
5355
9657
642
81.5
globlastp

LAB795_H6
barley|12v1|BI949478_P1
5356
9658
642
80.5
globlastp

LAB796_H1
maize|10v1|AI622188_P1
5357
9659
643
94.4
globlastp

LAB796_H2
foxtail_millet|11v3|PHY7SI0
5358
9660
643
89.6
globlastp

22955M_P1

LAB796_H3
switchgrass|12v1|FE644404_—
5359
9661
643
89.6
globlastp

P1

LAB796_H3
switchgrass|gb167|FE621433
5360
9662
643
88.4
globlastp

LAB796_H12
switchgrass|12v1|FE621433_—
5361
9663
643
88.1
globlastp

P1

LAB796_H4
brachypodium|12v1|BRADI2
5362
9664
643
83.2
globlastp

G23570_P1

LAB796_H5
barley|10v2|BI949038
5363
9665
643
82.9
globlastp

LAB796_H13
wheat|12v3|BQ903448_P1
5364
9666
643
82.6
globlastp

LAB796_H6
leymus|gb166|EG399451_P1
5365
9667
643
82.5
globlastp

LAB796_H8
wheat|12v3|BF484746_P1
5366
9668
643
82.5
globlastp

LAB796_H7
rice|11v1|BE229824
5367
9669
643
82.2
globlastp

LAB796_H8
wheat|10v2|BF484746
5368
9670
643
82.2
globlastp

LAB796_H9
millet|10v1|EVO454PM0377
5369
9671
643
81.7
globlastp

11_P1

LAB796_H11
wheat|12v3|BQ806352_P1
5370
9672
643
81.5
globlastp

LAB796_H10
rye|12v1|BF146028
5371
9673
643
80.7
globlastp

LAB796_H11
wheat|10v2|BQ806352
5372
9674
643
80.37
glotblastn

LAB797_H1
maize|10v1|AW267570_P1
5373
9675
644
96.5
globlastp

LAB797_H2
foxtail_millet|11v3|PHY7SI0
5374
9676
644
95
globlastp

05771M_P1

LAB797_H5
switchgrass|12v1|FL761828_—
5375
9677
644
94.5
globlastp

P1

LAB797_H3
brachypodium|12v1|BRADI1
5376
9678
644
89.2
globlastp

G52230_P1

LAB797_H4
rice|11v1|BM418583
5377
9679
644
88.51
glotblastn

LAB797_H6
rye|12v1|DRR001012.283982
5378
9680
644
80.8
globlastp

_P1

LAB797_H7
eucalyptus|11v2|SRR001659
5379
9681
644
80.5
globlastp

X105674_P1

LAB797_H8
grape|11v1|GSVIVT0103743
5380
9682
644
80
globlastp

7001_P1

LAB797_H9
watermelon|11v1|VMEL0022
5381
9683
644
80
globlastp

6737851839_P1

LAB798_H1
maize|10v1|AI820398_P1
5382
9684
645
89.1
globlastp

LAB798_H6
switchgrass|12v1|FE646593_—
5383
9685
645
88.1
globlastp

P1

LAB798_H2
sugarcane|10v1|CA138369
5384
9686
645
86
globlastp

LAB798_H3
foxtail_millet|11v3|PHY7SI0
5385
9687
645
84.9
globlastp

07347M_P1

LAB798_H4
switchgrass|gb167|FE613718
5386
9688
645
84.2
globlastp

LAB798_H4
switchgrass|12v1|FE613718_—
5387
9689
645
82.1
globlastp

P1

LAB798_H5
millet|10v1|EVO454PM1030
5388
9690
645
81.8
globlastp

80_P1

LAB798_H6
switchgrass|gb167|FE646593
5389
9691
645
80.7
globlastp

LAB799_H1
sorghum|12v1|SB03G033410
5390
9692
646
95.2
globlastp

LAB799_H2
maize|10v1|AI978026_P1
5391
9693
646
88.8
globlastp

LAB799_H4
switchgrass|12v1|FE624429_—
5392
9694
646
82.2
globlastp

P1

LAB799_H3
foxtail_millet|11v3|PHY7SI0
5393
9695
646
81.1
globlastp

21475M_P1

LAB799_H5
switchgrass|12v1|FL868889_—
5394
9696
646
80.7
globlastp

P1

LAB800_H1
pigeonpea|11v1|GW352087_—
5395
9697
647
96.2
globlastp

P1

LAB800_H2
soybean|11v1|GLYMA20G01
5396
9698
647
94.9
globlastp

930

LAB800_H2
soybean|12v1|GLYMA20G01
5397
9698
647
94.9
globlastp

930_P1

LAB800_H3
soybean|11v1|GLYMA06G05
5398
9699
647
94.3
globlastp

740

LAB800_H3
soybean|12v1|GLYMA06G05
5399
9699
647
94.3
globlastp

740_P1

LAB800_H84
bean|12v2|CA898282_P1
5400
9700
647
91.8
globlastp

LAB800_H4
bean|12v1|CA898282
5401
9700
647
91.8
globlastp

LAB800_H5
cowpea|12v1|FC460650_P1
5402
9701
647
91.1
globlastp

LAB800_H5
cowpea|gb166|FC460650
5403
9701
647
91.1
globlastp

LAB800_H6
trigonella|11v1|SRR066194X
5404
9702
647
88.1
globlastp

142200

LAB800_H7
lotus|09v1|BW602763_P1
5405
9703
647
86.2
globlastp

LAB800_H8
peanut|10v1|DT044355_P1
5406
9704
647
85.6
globlastp

LAB800_H9
chickpea|11v1|FE672070
5407
9705
647
85.5
globlastp

LAB800_H9
chickpea|13v2|FE672070_P1
5408
9705
647
85.5
globlastp

LAB800_H10
medicago|12v1|BG450559_P
5409
9706
647
85
globlastp

1

LAB800_H11
apple|11v1|CN868712_P1
5410
9707
647
84.8
globlastp

LAB800_H12
apple|11v1|CN994167_P1
5411
9707
647
84.8
globlastp

LAB800_H13
euonymus|11v1|SRR070038X
5412
9708
647
84.8
globlastp

104203_P1

LAB800_H14
grape|11v1|GSVIVT0103542
5413
9709
647
84.8
globlastp

9001_P1

LAB800_H15
pea|11v1|PEAHSP177A_P1
5414
9710
647
84.3
globlastp

LAB800_H16
walnuts|gb166|CB303500
5415
9711
647
84.3
globlastp

LAB800_H17
apple|11v1|CN445232_P1
5416
9712
647
84.2
globlastp

LAB800_H18
hevea|10v1|EC603261_P1
5417
9713
647
84.2
globlastp

LAB800_H19
cowpea|12v1|FF400274_P1
5418
9714
647
83.9
globlastp

LAB800_H19
cowpea|gb166|FF400274
5419
9714
647
83.9
globlastp

LAB800_H85
nicotiana_benthamiana|12v1|
5420
9715
647
83.6
globlastp

EB431431_P1

LAB800_H86
poplar|13v1|BU831700_P1
5421
9716
647
83.6
globlastp

LAB800_H20
jatropha|09v1|FM887248_P1
5422
9717
647
83.6
globlastp

LAB800_H21
apple|11v1|CN444285_P1
5423
9718
647
83.5
globlastp

LAB800_H22
euonymus|11v1|SRR070038X
5424
9719
647
83.5
globlastp

104955_P1

LAB800_H23
prunus|10v1|AF159562
5425
9720
647
83.5
globlastp

LAB800_H24
strawberry|11v1|DY667157
5426
9721
647
83.5
globlastp

LAB800_H25
cannabis|12v1|SOLX0007978
5427
9722
647
83.2
globlastp

2_P1

LAB800_H26,
castorbean|12v1|XM_002516
5428
9723
647
83.12
glotblastn

LAB800_H27
059_T1

LAB800_H87
castorbean|12v1|RIC12V1CU
5429
9724
647
83.1
globlastp

FF9072T1_P1

LAB800_H88
castorbean|12v1|XM_002516
5430
9724
647
83.1
globlastp

060_P1

LAB800_H27
castorbean|11v1|XM_002516
5431
9724
647
83.1
globlastp

060

LAB800_H28
catharanthus|11v1|AM232372
5432
9725
647
83.02
glotblastn

_T1

LAB800_H89
nicotiana_benthamiana|12v1|
5433
9726
647
83
globlastp

BP748306_P1

LAB800_H29
poplar|10v1|BU836876
5434
9727
647
83
globlastp

LAB800_H30
tobacco|gb162|AY329075
5435
9728
647
83
globlastp

LAB800_H31
tobacco|gb162|EB430311
5436
9729
647
83
globlastp

LAB800_H32
apple|11v1|CN868486_T1
5437
9730
647
82.91
glotblastn

LAB800_H90
prunus_mume|13v1|AF15956
5438
9731
647
82.9
globlastp

2_P1

LAB800_H33
beech|11v1|SRR006293.1347
5439
9732
647
82.6
globlastp

9_P1

LAB800_H34
pigeonpea|11v1|SRR054580X
5440
9733
647
82.5
globlastp

130347_P1

LAB800_H91
nicotiana_benthamiana|12v1|
5441
9734
647
82.4
globlastp

EB430305_P1

LAB800_H92
nicotiana_benthamiana|12v1|
5442
9735
647
82.4
globlastp

EB436662_P1

LAB800_H35
rose|12v1|BQ104014
5443
9736
647
82.4
globlastp

LAB800_H36
tabernaemontana|11v1|SRR09
5444
9737
647
82.4
globlastp

8689X11203

LAB800_H71
nicotiana_benthamiana|12v1|
5445
9738
647
82.4
globlastp

EB451782_P1

LAB800_H37
cacao|10v1|CA794569_P1
5446
9739
647
82.3
globlastp

LAB800_H38
cassava|09v1|DR084476_P1
5447
9740
647
82.3
globlastp

LAB800_H39
coffea|10v1|DV676785_P1
5448
9741
647
82.3
globlastp

LAB800_H40
grape|11v1|GSVIVT0103543
5449
9742
647
82.3
globlastp

6001_P1

LAB800_H41
nasturtium|11v1|SRR032558.
5450
9743
647
82
globlastp

100245_P1

LAB800_H42
nasturtium|11v1|SRR032558.
5451
9743
647
82
globlastp

102872_P1

LAB800_H43
humulus|11v1|EX521136_P1
5452
9744
647
81.9
globlastp

LAB800_H93
nicotiana_benthamiana|12v1|
5453
9745
647
81.8
globlastp

DV999708_P1

LAB800_H94
nicotiana_benthamiana|12v1|
5454
9746
647
81.8
globlastp

EB430311_P1

LAB800_H95
olea|13v1|SRR592583X10394
5455
9747
647
81.8
globlastp

7D1_P1

LAB800_H44
fraxinus|11v1|SRR058827.10
5456
9748
647
81.76
glotblastn

3419_T1

LAB800_H45
eucalyptus|11v2|SRR001659
5457
9749
647
81.6
globlastp

X280547_P1

LAB800_H46
watermelon|11v1|CLICUGI11
5458
9750
647
81.6
globlastp

014604

LAB800_H47
watermelon|11v1|DV632800
5459
9750
647
81.6
globlastp

LAB800_H48
lotus|09v1|CRPLJ032993_P1
5460
9751
647
81.2
globlastp

LAB800_H49
tomato|11v1|LEU72396
5461
9752
647
81.2
globlastp

LAB800_H50
monkeyflower|12v1|GR09012
5462
9753
647
81.13
glotblastn

0_T1

LAB800_H96
monkeyflower|12v1|GO98065
5463
9754
647
81.1
globlastp

0_P1

LAB800_H97
nicotiana_benthamiana|12v1|
5464
9755
647
81.1
globlastp

AY329075_P1

LAB800_H50
monkeyflower|10v1|GO98064
5465
9754
647
81.1
globlastp

9

LAB800_H51
orobanche|10v1|SRR023189S
5466
9756
647
81.1
globlastp

0028578_P1

LAB800_H52
tobacco|gb162|DV999708
5467
9757
647
81.1
globlastp

LAB800_H53
tobacco|gb162|EB435563
5468
9758
647
81.1
globlastp

LAB800_H54
walnuts|gb166|EL894787
5469
9759
647
81.1
globlastp

LAB800_H55
cucurbita|11v1|SRR091276X
5470
9760
647
81.01
glotblastn

219629_T1

LAB800_H56
eucalyptus|11v2|SRR001659
5471
9761
647
81
globlastp

X164791_P1

LAB800_H57
grape|11v1|GSVIVT0103543
5472
9762
647
81
globlastp

1001_P1

LAB800_H58
nasturtium|11v1|GH164946_P
5473
9763
647
80.9
globlastp

1

LAB800_H59
clementine|11v1|BQ624198_—
5474
9764
647
80.86
glotblastn

T1

LAB800_H60
orange|11v1|BQ624198_T1
5475
9765
647
80.86
glotblastn

LAB800_H61
nasturtium|11v1|SRR032558.
5476
9766
647
80.75
glotblastn

103689_T1

LAB800_H62
beech|11v1|SRR006293.1294
5477
9767
647
80.7
globlastp

1_P1

LAB800_H63
lotus|09v1|CRPLJ008753_P1
5478
9768
647
80.7
globlastp

LAB800_H64
nasturtium|11v1|SRR032558.
5479
9769
647
80.7
globlastp

100084_P1

LAB800_H65
soybean|11v1|GLYMA14G11
5480
9770
647
80.7
globlastp

420

LAB800_H65
soybean|12v1|GLYMA14G11
5481
9770
647
80.7
globlastp

420_P1

LAB800_H98
bean|12v2|CA906174_P1
5482
9771
647
80.6
globlastp

LAB800_H99
bean|12v2|CA906176_P1
5483
9772
647
80.6
globlastp

LAB800_H66
bean|12v1|CA906176
5484
9772
647
80.6
globlastp

LAB800_H67
euphorbia|11v1|DV120470_P
5485
9773
647
80.6
globlastp

1

LAB800_H68
solanum_phureja|09v1|SPHAI
5486
9774
647
80.6
globlastp

776971

LAB800_H69
spurge|gb161|DV120470
5487
9773
647
80.6
globlastp

LAB800_H70
amsonia|11v1|SRR098688X1
5488
9775
647
80.5
globlastp

86168_P1

LAB800_H71
nicotiana_benthamiana|gb162|
5489
9776
647
80.5
glotblastn

ES886792

LAB800_H72
orobanche|10v1|SRR023189S
5490
9777
647
80.5
globlastp

0041905_P1

LAB800_H73
papaya|gb165|AY242075_P1
5491
9778
647
80.5
globlastp

LAB600_H14
grape|11v1|GSVIVT0103543
5492
9779
647
80.4
globlastp

3001_P1

LAB800_H74
cotton|11v1|BE053976_P1
5493
9780
647
80.4
globlastp

LAB800_H75
cotton|11v1|CO080002_P1
5494
9781
647
80.4
globlastp

LAB800_H76
gossypium_raimondii|12v1|B
5495
9780
647
80.4
globlastp

E053976_P1

LAB800_H77
grape|11v1|GSVIVT0103542
5496
9782
647
80.38
glotblastn

7001_T1

LAB800_H78
hornbeam|12v1|SRR364455.1
5497
9783
647
80.38
glotblastn

17533_T1

LAB800_H79
beech|11v1|SRR364434.1743
5498
9784
647
80.12
glotblastn

07_T1

LAB800_H80
oak|10v1|FP056783_T1
5499
9785
647
80.12
glotblastn

LAB800_H81
nasturtium|11v1|SRR032558.
5500
9786
647
80.1
globlastp

215294_P1

LAB800_H82
soybean|11v1|GLYMA14G11
5501
9787
647
80.1
globlastp

430

LAB800_H82
soybean|12v1|GLYMA14G11
5502
9787
647
80.1
globlastp

430_P1

LAB800_H10
pepper|12v1|CA522182_P1
5503
9788
647
80
globlastp

0

LAB800_H83
solanum_phureja|09v1|SPHL
5504
9789
647
80
globlastp

EU72396

LAB801_H1
soybean|11v1|GLYMA06G48
5505
9790
648
91.8
globlastp

220

LAB801_H1
soybean|12v1|GLYMA06G48
5506
9790
648
91.8
globlastp

220_P1

LAB801_H2
pigeonpea|11v1|SRR054580X
5507
9791
648
86.9
globlastp

100068_P1

LAB801_H3
lotus|09v1|LLAW720413_P1
5508
9792
648
85.9
globlastp

LAB801_H5
bean|12v2|SRR001334.17602
5509
9793
648
83.6
globlastp

8_P1

LAB801_H6
chickpea|13v2|SRR133517.14
5510
9794
648
82.6
globlastp

7443_P1

LAB802_H1
soybean|11v1|GLYMA08G23
5511
9795
649
94.9
globlastp

980

LAB802_H1
soybean|12v1|GLYMA08G23
5512
9795
649
94.9
globlastp

980_P1

LAB802_H2
pigeonpea|11v1|SRR054580X
5513
9796
649
89.7
globlastp

104838_P1

LAB802_H13
bean|12v2|CA900965_T1
5514
9797
649
89.05
glotblastn

LAB802_H3
bean|12v1|CA900965
5515
9798
649
89.05
glotblastn

LAB802_H4
chickpea|11v1|FE671183
5516
9799
649
87.6
globlastp

LAB802_H4
chickpea|13v2|FE671183_P1
5517
9799
649
87.6
globlastp

LAB802_H5
cowpea|12v1|FF384470_P1
5518
9800
649
87.6
globlastp

LAB802_H5
cowpea|gb166|FF384470
5519
9800
649
87.6
globlastp

LAB802_H14
chickpea|13v2|GR393338_T1
5520
9801
649
87.59
glotblastn

LAB802_H6
medicago|12v1|AL388023_P1
5521
9802
649
86.8
globlastp

LAB802_H7
trigonella|11v1|SRR066194X
5522
9803
649
86.8
globlastp

225634

LAB802_H8
cyamopsis|10v1|EG975063_P
5523
9804
649
85.5
globlastp

1

LAB802_H9
lotus|09v1|LLGO006934_P1
5524
9805
649
84.7
globlastp

LAB802_H10
peanut|10v1|SRR042421S003
5525
9806
649
81.9
globlastp

9205_P1

LAB802_H11
peanut|10v1|GO330236_P1
5526
9807
649
80.4
globlastp

LAB802_H12
acacia|10v1|FS584980_P1
5527
9808
649
80.3
globlastp

LAB803_H4
bean|12v2|SRR001335.33140
5528
9809
650
82.7
globlastp

1_P1

LAB803_H1
bean|12v1|SRR001334.15231
5529
9809
650
82.7
globlastp

4

LAB803_H2
pigeonpea|11v1|SRR054580X
5530
9810
650
80.7
globlastp

100809_P1

LAB803_H3
pigeonpea|11v1|SRR054580X
5531
9811
650
80.3
globlastp

214670_P1

LAB804_H26
cyamopsis|10v1|EG983307_P
5532
9812
651
97.6
globlastp

1

LAB804_H27
cowpea|12v1|FC458549_P1
5533
9813
651
97.1
globlastp

LAB804_H27
cowpea|gb166|FC458549
5534
9813
651
97.1
globlastp

LAB804_H11
bean|12v2|CA898997_P1
5535
9814
651
96.6
globlastp

1

LAB804_H28
bean|12v1|CA898997
5536
9814
651
96.6
globlastp

LAB804_H29
liquorice|gb171|FS243799_P1
5537
9815
651
91.8
globlastp

LAB804_H30
chickpea|13v2|FE671625_P1
5538
9816
651
90.4
globlastp

LAB804_H30
chickpea|11v1|FE671625
5539
9817
651
90.38
glotblastn

LAB804_H31
lotus|09v1|LLAI967448_P1
5540
9818
651
89.9
globlastp

LAB804_H32
trigonella|11v1|SRR066194X
5541
9819
651
89.9
globlastp

125989

LAB804_H33
medicago|12v1|BF519737_P1
5542
9820
651
89.4
globlastp

LAB804_H34
pea|11v1|GH720686_P1
5543
9821
651
88.9
globlastp

LAB804_H35
poplar|10v1|BU821970
5544
9822
651
88.5
globlastp

LAB804_H35
poplar|13v1|BU821970_P1
5545
9823
651
88.5
globlastp

LAB804_H11
prunus_mume|13v1|DY64090
5546
9824
651
87.3
globlastp

2
2_P1

LAB804_H36
castorbean|12v1|EE254317_P
5547
9825
651
87.1
globlastp

1

LAB804_H37
prunus|10v1|CN496867
5548
9826
651
86.9
globlastp

LAB804_H38
cassava|09v1|DV449879_P1
5549
9827
651
86.6
globlastp

LAB804_H39
hevea|10v1|EC601072_P1
5550
9828
651
86.6
globlastp

LAB804_H40
scabiosa|11v1|SRR063723X1
5551
9829
651
86.5
globlastp

062

LAB804_H41
apple|11v1|CN861796_P1
5552
9830
651
86.4
globlastp

LAB804_H42
rose|12v1|BQ105267
5553
9831
651
86.4
globlastp

LAB804_H43
centaurea|gb166|EH751391_P
5554
9832
651
86.1
globlastp

1

LAB804_H44
flax|11v1|GW863971_T1
5555
9833
651
85.71
glotblastn

LAB804_H45
ambrosia|11v1|SRR346935.2
5556
9834
651
85.6
globlastp

60059_P1

LAB804_H46
ambrosia|11v1|SRR346943.1
5557
9834
651
85.6
globlastp

00164_P1

LAB804_H47
arnica|11v1|SRR099034X110
5558
9835
651
85.6
globlastp

457_P1

LAB804_H48
cirsium|11v1|SRR346952.100
5559
9836
651
85.6
globlastp

4150_P1

LAB804_H49
dandelion|10v1|DR400376_P
5560
9837
651
85.6
globlastp

1

LAB804_H50
euphorbia|11v1|BP961242_P1
5561
9838
651
85.6
globlastp

LAB804_H51
euphorbia|11v1|DV113328_P
5562
9839
651
85.6
globlastp

1

LAB804_H52
flaveria|11v1|SRR149229.432
5563
9840
651
85.6
globlastp

41_P1

LAB804_H53
acacia|10v1|FS586585_P1
5564
9841
651
85.4
globlastp

LAB804_H54
cassava|09v1|CK646847_P1
5565
9842
651
85.2
globlastp

LAB804_H55
centaurea|gb166|EH724339_P
5566
9843
651
85.1
globlastp

1

LAB804_H56
cichorium|gb171|EH699860_—
5567
9844
651
85.1
globlastp

P1

LAB804_H57
valeriana|11v1|SRR099039X1
5568
9845
651
85.1
glotblastn

18027

LAB804_H58
apple|11v1|CN496867_P1
5569
9846
651
85
globlastp

LAB804_H59
kiwi|gb166|FG435327_P1
5570
9847
651
85
globlastp

LAB804_H11
lettuce|12v1|DW055702_P1
5571
9848
651
84.6
globlastp

3

LAB804_H60
chestnut|gb170|SRR006295S0
5572
9849
651
84.6
globlastp

045248_P1

LAB804_H61
flaveria|11v1|SRR149232.106
5573
9850
651
84.6
globlastp

461_P1

LAB804_H62
lettuce|10v1|DW055702
5574
9848
651
84.6
globlastp

LAB804_H63
sunflower|12v1|CD849704
5575
9851
651
84.6
globlastp

LAB804_H64
euonymus|11v1|SRR070039X
5576
9852
651
84.5
globlastp

102276_P1

LAB804_H65
valeriana|11v1|SRR099039X1
5577
9853
651
84.13
glotblastn

36746

LAB804_H66
beech|11v1|SRR006293.1633
5578
9854
651
84.1
globlastp

1_P1

LAB804_H67
cucurbita|11v1|SRR091276X
5579
9855
651
84
globlastp

100208_P1

LAB804_H68
tabernaemontana|11v1|SRR09
5580
9856
651
84
globlastp

8689X110632

LAB804_H69
watermelon|11v1|AM718428
5581
9857
651
84
globlastp

LAB804_H70
tragopogon|10v1|SRR020205
5582
9858
651
83.7
globlastp

S0025271

LAB804_H71
tea|10v1|CV013617
5583
9859
651
83.65
glotblastn

LAB804_H72
monkeyflower|10v1|DV20949
5584
9860
651
83.6
globlastp

4

LAB804_H72
monkeyflower|12v1|DV20949
5585
9860
651
83.6
globlastp

4_P1

LAB804_H73
strawberry|11v1|EX658292
5586
9861
651
83.6
globlastp

LAB804_H11
blueberry|12v1|SRR353282X
5587
9862
651
83.5
globlastp

4
10613D1_P1

LAB804_H74
artemisia|10v1|GW328359_P
5588
9863
651
83.2
globlastp

1

LAB804_H75
senecio|gb170|DY661167
5589
9864
651
83.2
globlastp

LAB804_H11
aquilegia|10v2|DR920468_P1
5590
9865
651
83.1
globlastp

5

LAB804_H76
amsonia|11v1|SRR098688X1
5591
9866
651
83.1
globlastp

07893_P1

LAB804_H77
aquilegia|10v1|DR920468
5592
9865
651
83.1
globlastp

LAB804_H78
basilicum|10v1|DY326359_P
5593
9867
651
83.1
globlastp

1

LAB804_H79
catharanthus|11v1|EG561880
5594
9868
651
83.1
globlastp

_P1

LAB804_H80
potato|10v1|BE923319_P1
5595
9869
651
83.1
globlastp

LAB804_H81
tomato|11v1|BG127204
5596
9869
651
83.1
globlastp

LAB804_H82
triphysaria|10v1|DR171477
5597
9870
651
83.1
globlastp

LAB804_H83
vinca|11v1|SRR098690X1603
5598
9871
651
82.7
globlastp

93

LAB804_H11
nicotiana_benthamiana|12v1|
5599
9872
651
82.6
globlastp

6
EB425761_P1

LAB804_H11
sesame|12v1|JK082889_P1
5600
9873
651
82.6
globlastp

7

LAB804_H84
eggplant|10v1|FS020089_P1
5601
9874
651
82.6
globlastp

LAB804_H85
phyla|11v2|SRR099037X129
5602
9875
651
82.6
globlastp

737_P1

LAB804_H86
solanum_phureja|09v1|SPHB
5603
9876
651
82.6
globlastp

G127204

LAB804_H87
fagopyrum|11v1|SRR063689
5604
9877
651
82.3
globlastp

X12687_P1

LAB804_H88
vinca|11v1|SRR098690X1003
5605
9878
651
82.3
globlastp

16

LAB804_H89
flaveria|11v1|SRR149241.124
5606
9879
651
82.21
glotblastn

263_T1

LAB804_H90
guizotia|10v1|GE553427_T1
5607
9880
651
82.21
glotblastn

LAB804_H11
pepper|12v1|BM065058_P1
5608
9881
651
82.2
globlastp

8

LAB804_H91
fraxinus|11v1|SRR058827.10
5609
9882
651
82.2
globlastp

2106_P1

LAB804_H92
kiwi|gb166|FG408013_P1
5610
9883
651
82.2
globlastp

LAB804_H93
melon|10v1|AM718428_P1
5611
9884
651
82.2
globlastp

LAB804_H94
olea|11v1|SRR014463.31738
5612
9885
651
82.2
globlastp

LAB804_H94
olea|13v1|SRR014463X31738
5613
9885
651
82.2
globlastp

D1_P1

LAB804_H95
pepper|gb171|BM065058
5614
9881
651
82.2
globlastp

LAB804_H96
petunia|gb171|CV295360_P1
5615
9886
651
82.2
globlastp

LAB804_H97
antirrhinum|gb166|AJ797616
5616
9887
651
82.16
glotblastn

_T1

LAB804_H11
nicotiana_benthamiana|12v1|
5617
9888
651
81.7
globlastp

9
DV158338_P1

LAB804_H98
platanus|11v1|SRR096786X1
5618
9889
651
81.7
globlastp

93192_P1

LAB804_H99
salvia|10v1|FE536438
5619
9890
651
81.7
globlastp

LAB804_H10
tobacco|gb162|EB425761
5620
9891
651
81.7
globlastp

0

LAB804_H10
fagopyrum|11v1|SRR063703
5621
9892
651
81.34
glotblastn

1
X119223_T1

LAB804_H10
arnica|11v1|SRR099034X119
5622
9893
651
81.2
globlastp

2
831_P1

LAB804_H10
liriodendron|gb166|CK76152
5623
9894
651
81.2
globlastp

3
5_P1

LAB804_H10
silene|11v1|SRR096785X107
5624
9895
651
81.2
globlastp

4
416XX1

LAB804_H10
utricularia|11v1|SRR094438.1
5625
9896
651
81.2
globlastp

5
05376

LAB804_H10
plantago|11v2|SRR066373Xl
5626
9897
651
81.04
glotblastn

6
19026_T1

LAB804_H10
ipomoea_batatas|10v1|EE880
5627
9898
651
80.8
globlastp

7
037_P1

LAB804_H10
chelidonium|11v1|SRR08475
5628
9899
651
80.75
glotblastn

8
2X105206_T1

LAB804_H10
coffea|10v1|DV665876_P1
5629
9900
651
80.6
globlastp

9

LAB804_H12
olea|13v1|SRR014463X10905
5630
9901
651
80.3
globlastp

0
D1_P1

LAB804_H11
beet|12v1|EG551047_P1
5631
9902
651
80.1
globlastp

0

LAB805_H1
soybean|11v1|GLYMA13G20
5632
9903
652
89.8
globlastp

830

LAB805_H1
soybean|12v1|GLYMA13G20
5633
9903
652
89.8
globlastp

830_P1

LAB805_H2
pigeonpea|11v1|SRR054580X
5634
9904
652
87.9
globlastp

103638_P1

LAB807_H76
soybean|12v1|GLYMA20G28
5635
9905
653
96.2
globlastp

900_P1

LAB807_H1
soybean|11v1|GLYMA20G28
5636
9905
653
96.2
globlastp

900

LAB807_H2
pigeonpea|11v1|SRR054580X
5637
9906
653
94.3
globlastp

125671_P1

LAB807_H77
bean|12v2|CA898563_P1
5638
9907
653
92.9
globlastp

LAB807_H3
bean|12v1|CA898563
5639
9907
653
92.9
globlastp

LAB807_H4
cowpea|12v1|FF383219_P1
5640
9908
653
92.9
globlastp

LAB807_H4
cowpea|gb166|FF383219
5641
9908
653
92.9
globlastp

LAB807_H5
cyamopsis|10v1|EG975757_P
5642
9909
653
92.9
globlastp

1

LAB807_H6
peanut|10v1|ES711872_P1
5643
9910
653
90.3
globlastp

LAB807_H7
medicago|12v1|AL372542_P1
5644
9911
653
88.7
globlastp

LAB807_H8
lotus|09v1|GO005260_P1
5645
9912
653
88.3
globlastp

LAB807_H9
chickpea|11v1|FE671026
5646
9913
653
87
globlastp

LAB807_H9
chickpea|13v2|FE671026_P1
5647
9913
653
87
globlastp

LAB807_H10
trigonella|11v1|SRR066194X
5648
9914
653
86.6
globlastp

10284XX1

LAB807_H11
cannabis|12v1|SOLX0001394
5649
9915
653
83.1
globlastp

9_P1

LAB807_H12
cacao|10v1|CU519533_P1
5650
9916
653
83
globlastp

LAB807_H13
heritiera|10v1|SRR005795S00
5651
9917
653
83
globlastp

02179_P1

LAB807_H78
poplar|13v1|BU818914_P1
5652
9918
653
82.9
globlastp

LAB807_H14
momordica|10v1|SRR071315
5653
9919
653
82.9
globlastp

S0002442_P1

LAB807_H15
salvia|10v1|CV162575
5654
9920
653
82.86
glotblastn

LAB807_H16
beech|11v1|FR602378_P1
5655
9921
653
82.7
globlastp

LAB807_H17
chestnut|gb170|SRR006295S0
5656
9922
653
82.6
globlastp

010330_P1

LAB807_H18
tabernaemontana|11v1|SRR09
5657
9923
653
82.55
glotblastn

8689X118501

LAB807_H19
monkeyflower|10v1|GR00015
5658
9924
653
82.46
glotblastn

7

LAB807_H20
cassava|09v1|DV441208_P1
5659
9925
653
82.4
globlastp

LAB807_H21
watermelon|11v1|VMEL0079
5660
9926
653
82.4
globlastp

8506531714

LAB807_H22
tripterygium|11v1|SRR09867
5661
9927
653
82.16
glotblastn

7X117580

LAB807_H19
monkeyflower|12v1|GR02544
5662
9928
653
81.99
glotblastn

4_T1

LAB807_H23
castorbean|11v1|EE254989
5663
9929
653
81.9
globlastp

LAB807_H23
castorbean|12v1|EE254989_P
5664
9929
653
81.9
globlastp

1

LAB807_H24
papaya|gb165|EX267214_P1
5665
9930
653
81.9
globlastp

LAB807_H25
oak|10v1|CU657001_P1
5666
9931
653
81.7
globlastp

LAB807_H26
amsonia|11v1|SRR098688X1
5667
9932
653
81.6
glotblastn

24319_T1

LAB807_H27
b_rapa|11v1|L46438_T1
5668
9933
653
81.6
glotblastn

LAB807_H28
gerbera|09v1|AJ750112_P1
5669
9934
653
81.6
globlastp

LAB807_H29
vinca|11v1|SRR098690X1007
5670
9935
653
81.6
glotblastn

73

LAB807_H79
olea|13v1|SRR014464X39879
5671
9936
653
81.43
glotblastn

D1_T1

LAB807_H30
rhizophora|10v1|SRR005792S
5672
9937
653
81.43
glotblastn

0007617

LAB807_H73
olea|13v1|SRR014463X59571
5673
9938
653
81.43
glotblastn

D1_T1

LAB807_H31
cleome_spinosa|10v1|GR9321
5674
9939
653
81.4
globlastp

25_P1

LAB807_H32
cucurbita|11v1|SRR091276X
5675
9940
653
81.4
globlastp

106181_P1

LAB807_H33
cotton|11v1|AI727096_P1
5676
9941
653
81.2
globlastp

LAB807_H34
catharanthus|11v1|SRR09869
5677
9942
653
81.13
glotblastn

1X123316_T1

LAB807_H35
vinca|11v1|SRR098690X2354
5678
9943
653
81.13
glotblastn

82

LAB807_H36
flaveria|11v1|SRR149229.113
5679
9944
653
81.1
globlastp

015_P1

LAB807_H37
ipomoea_batatas|10v1|CB330
5680
9945
653
81.1
globlastp

389_P1

LAB807_H38
thellungiella_parvulum|11v1|
5681
9946
653
81.04
glotblastn

BY807252

LAB807_H39
tragopogon|10v1|SRR020205
5682
9947
653
81.04
glotblastn

S0017796

LAB807_H40
euphorbia|11v1|SRR098678X
5683
9948
653
81
globlastp

112186_P1

LAB807_H41
valeriana|11v1|SRR099039X1
5684
9949
653
80.95
glotblastn

16893

LAB807_H42
antirrhinum|gb166|AJ559323
5685
9950
653
80.8
globlastp

_P1

LAB807_H80
sesame|12v1|SESI12V137350
5686
9951
653
80.7
globlastp

5_P1

LAB807_H43
cynara|gb167|GE591023_P1
5687
9952
653
80.7
globlastp

LAB807_H44
orobanche|10v1|SRR023189S
5688
9953
653
80.7
globlastp

0007423_P1

LAB807_H45
phyla|11v2|SRR099035X112
5689
9954
653
80.7
globlastp

372_P1

LAB807_H46
cleome_spinosa|10v1|GR9325
5690
9955
653
80.6
globlastp

45_P1

LAB807_H47
poppy|11v1|FE964957_P1
5691
9956
653
80.6
globlastp

LAB807_H48
centaurea|gb166|EH716099_T
5692
9957
653
80.57
glotblastn

1

LAB807_H49
centaurea|gb166|EH760760_T
5693
9958
653
80.57
glotblastn

1

LAB807_H50
cichorium|gb171|EH678948_—
5694
9959
653
80.57
glotblastn

T1

LAB807_H51
cirsium|11v1|SRR346952.103
5695
9960
653
80.57
glotblastn

242_T1

LAB807_H52
safflower|gb162|EL377033
5696
9961
653
80.57
glotblastn

LAB807_H53
melon|10v1|VMEL00061337
5697
9962
653
80.5
globlastp

043403_P1

LAB807_H54
eschscholzia|11v1|SRR01411
5698
9963
653
80.48
glotblastn

6.12846_T1

LAB807_H55
fraxinus|11v1|SRR058827.10
5699
9964
653
80.48
glotblastn

9687_T1

LAB807_H56
rose|12v1|EC588505
5700
9965
653
80.48
glotblastn

LAB807_H57
ipomoea_nil|10v1|BJ560618_—
5701
9966
653
80.3
globlastp

P1

LAB807_H58
cirsium|11v1|SRR346952.100
5702
9967
653
80.2
globlastp

4278_P1

LAB807_H59
cleome_gynandra|10v1|SRR0
5703
9968
653
80.2
globlastp

15532S0016159_P1

LAB807_H60
flaveria|11v1|SRR149229.266
5704
9969
653
80.2
globlastp

171_P1

LAB807_H61
hornbeam|12v1|SRR364455.1
5705
9970
653
80.2
globlastp

03918_P1

LAB807_H62
sarracenia|11v1|SRR192669.1
5706
9971
653
80.19
glotblastn

33211

LAB807_H63
thellungiella_halophilum|11v
5707
9972
653
80.19
glotblastn

1|BY807252

LAB807_H64
apple|11v1|CN908808_P1
5708
9973
653
80.1
globlastp

LAB807_H65
grape|11v1|GSVIVT0101804
5709
9974
653
80.1
globlastp

9001_P1

LAB807_H81
lettuce|12v1|DW051953_T1
5710
9975
653
80.09
glotblastn

LAB807_H66
blueberry|10v1|CF810446
5711
9976
653
80.09
glotblastn

LAB807_H66
blueberry|12v1|CF810446_T1
5712
9977
653
80.09
glotblastn

LAB807_H67
dandelion|10v1|DR399867_T
5713
9978
653
80.09
glotblastn

1

LAB807_H68
lettuce|10v1|DW051953
5714
9975
653
80.09
glotblastn

LAB807_H69
cucumber|09v1|BGI454H003
5715
9979
653
80
globlastp

5161_P1

LAB807_H70
euphorbia|11v1|DV131535_P
5716
9980
653
80
globlastp

1

LAB807_H71
gossypium_raimondii|12v1|AI
5717
9981
653
80
glotblastn

054902_T1

LAB807_H72
liriodendron|gb166|CK75932
5718
9982
653
80
glotblastn

0_T1

LAB807_H73
olea|11v1|SRR014463.59571
5719
9983
653
80
globlastp

LAB807_H74
platanus|11v1|SRR096786X1
5720
9984
653
80
globlastp

04408_P1

LAB807_H75
triphysarial|10v1|EY129212
5721
9985
653
80
glotblastn

LAB809_H1
cowpea|12v1|FF387716_P1
5722
9986
654
88.4
globlastp

LAB809_H1
cowpea|gb166|FF387716
5723
9986
654
88.4
globlastp

LAB810_H3
cowpea|12v1|FF393926_P1
5724
9987
655
89.6
globlastp

LAB810_H1
pigeonpea|11v1|SRR054580X
5725
9988
655
88.5
globlastp

149151_P1

LAB810_H4
bean|12v2|CB539392_P1
5726
9989
655
88.4
globlastp

LAB810_H2
bean|12v1|CB539392
5727
9989
655
88.4
globlastp

LAB810_H3
cowpea|gb166|FF393926
5728
9990
655
83
globlastp

LAB811_H3
bean|12v2|HO779763_P1
5729
9991
656
83.7
globlastp

LAB811_H2
pigeonpea|11v1|SRR054580X
5730
9992
656
83.2
globlastp

120722_P1

LAB813_H2
flaveria|11v1|SRR149232.586
5731
9993
657
86.5
globlastp

94_P1

LAB813_H3
arnica|11v1|SRR099034X150
5732
9994
657
85.7
globlastp

573_P1

LAB813_H4
flaveria|11v1|SRR149229.127
5733
9995
657
85
globlastp

958_P1

LAB813_H6
flaveria|11v1|SRR149229.231
5734
9996
657
83.5
globlastp

209_P1

LAB813_H7
cynara|gb167|GE586857_P1
5735
9997
657
82.7
globlastp

LAB813_H8
gerbera|09v1|AJ759966_P1
5736
9998
657
81.2
globlastp

LAB815_H1
ambrosia|11v1|SRR346935.3
5737
9999
659
87.3
globlastp

71050XX1_P1

LAB815_H2
ambrosia|11v1|SRR346935.3
5738
9999
659
87.3
globlastp

71050XX2_P1

LAB815_H3
flaveria|11v1|SRR149229.130
5739
10000
659
86.2
globlastp

401_P1

LAB815_H4
flaveria|11v1|SRR149232.147
5740
10001
659
84.1
globlastp

918_P1

LAB817_H1
parthenium|10v1|GW778454_—
5741
10002
661
94.8
globlastp

P1

LAB817_H2
guizotia|10v1|GE568746_T1
5742
10003
661
89.01
glotblastn

LAB817_H3
flaveria|11v1|SRR149229.111
5743
10004
661
86
globlastp

492_P1

LAB817_H4
ambrosia|11v1|SRR346935.1
5744
10005
661
85.2
globlastp

55079_P1

LAB820_H1
ambrosia|11v1|SRR346935.1
5745
10006
662
94.7
globlastp

02627XX2_P1

LAB820_H2
ambrosia|11v1|SRR346935.1
5746
10007
662
94.5
globlastp

02627XX1_P1

LAB820_H3
arnica|11v1|SRR099034X107
5747
10008
662
92.8
globlastp

213_P1

LAB820_H4
flaveria|11v1|SRR149232.208
5748
10009
662
91.87
glotblastn

106_T1

LAB820_H5
flaveria|11v1|SRR149241.258
5749
10010
662
91.3
globlastp

488_P1

LAB820_H6
flaveria|11v1|SRR149229.110
5750
10011
662
90.3
globlastp

735_P1

LAB820_H7
cynara|gb167|GE580304_T1
5751
10012
662
88.14
glotblastn

LAB820_H15
lettuce|12v1|DW056285_P1
5752
10013
662
87.7
globlastp

LAB820_H8
centaurea|gb166|EH717678_P
5753
10014
662
87.2
globlastp

1

LAB820_H9
cirsium|11v1|SRR346952.111
5754
10015
662
86.1
globlastp

862_P1

LAB820_H10
cirsium|11v1|SRR346952.109
5755
10016
662
85.2
globlastp

4403_P1

LAB820_H11
conyza|10v1|SRR035294S000
5756
10017
662
85
globlastp

2216_P1

LAB820_H12
artemisia|10v1|EY072500_P1
5757
10018
662
83.8
globlastp

LAB820_H13
safflower|gb162|EL378890
5758
10019
662
82.9
globlastp

LAB820_H14
cirsium|11v1|SRR346952.154
5759
10020
662
81.5
globlastp

085_P1

LAB824_H1
potato|10v1|BE924463_P1
5760
10021
665
95.4
globlastp

LAB824_H2
solanum_phureja|09v1|SPHAI
5761
10022
665
95.1
globlastp

773686

LAB824_H3
tobacco|gb162|CV018980
5762
10023
665
89.5
globlastp

LAB824_H19
nicotiana_benthamiana|12v1|
5763
10024
665
88.8
globlastp

EB425396_P1

LAB824_H20
nicotiana_benthamiana|12v1|
5764
10025
665
88.1
globlastp

EB443773_P1

LAB824_H4
tomato|11v1|AI484585
5765
10026
665
88.1
globlastp

LAB824_H21
pepper|12v1|CF270167_P1
5766
10027
665
87.4
globlastp

LAB824_H5
pepper|gb171|CF270167
5767
10028
665
87.4
globlastp

LAB824_H6
solanum_phureja|09v1|SPHAI
5768
10029
665
87.1
globlastp

484585

LAB824_H7
catharanthus|11v1|SRR09869
5769
10030
665
86.7
globlastp

1X111577XX2_P1

LAB824_H22
nicotiana_benthamiana|12v1|
5770
10031
665
86
globlastp

FS406104_P1

LAB824_H23
sesame|12v1|BU670215_P1
5771
10032
665
84.3
globlastp

LAB824_H8
monkeyflower|10v1|CV51546
5772
10033
665
83.9
globlastp

0

LAB824_H8
monkeyflower|12v1|CV51546
5773
10033
665
83.9
globlastp

0_P1

LAB824_H9
monkeyflower|12v1|GO95917
5774
10034
665
83.2
globlastp

8_P1

LAB824_H9
monkeyflower|10v1|GO95598
5775
10035
665
82.8
globlastp

6

LAB824_H10
plantago|11v2|SRR066373X1
5776
10036
665
82.8
globlastp

11857_P1

LAB824_H11
fraxinus|11v1|SRR058827.10
5777
10037
665
82.5
globlastp

0103_P1

LAB824_H12
tabernaemontana|11v1|SRR09
5778
10038
665
82.5
globlastp

8689X124168

LAB824_H13
amsonia|11v1|SRR098688X1
5779
10039
665
82.1
globlastp

06403_P1

LAB824_H14
ipomoea_nil|10v1|CJ750973_—
5780
10040
665
81.8
globlastp

P1

LAB824_H15
monkeyflower|10v1|SRR0372
5781
10041
665
81.4
glotblastn

28S0189464

LAB824_H16
phyla|11v2|SRR099035X100
5782
10042
665
81.12
glotblastn

790_T1

LAB824_H17
triphysaria|10v1|EX988972
5783
10043
665
81.1
globlastp

LAB824_H18
fraxinus|11v1|SRR058827.10
5784
10044
665
80.4
globlastp

1599_P1

LAB824_H24
blueberry|12v1|CF811616_P1
5785
10045
665
80
globlastp

LAB825_H1
solanum_phureja|09v1|SPHAI
5786
10046
666
92
globlastp

778113

LAB825_H2
pepper|gb171|BM063860
5787
10047
666
85.6
globlastp

LAB825_H3
nicotiana_benthamiana|12v1|
5788
10048
666
84.3
globlastp

EB692345_P1

LAB825_H4
pepper|12v1|BM063860_P1
5789
10049
666
84
globlastp

LAB827_H1
potato|10v1|BF052863_P1
5790
10050
667
90.2
globlastp

LAB827_H2
solanum_phureja|09v1|SPHA
5791
10051
667
84.9
globlastp

W035009

LAB829_H1
solanum_phureja|09v1|SPHA
5792
10052
668
99
globlastp

W217661

LAB829_H2
tobacco|gb162|DQ340760
5793
10053
668
90.8
globlastp

LAB829_H7
nicotiana_benthamiana|12v1|
5794
10054
668
86.8
globlastp

DQ340760_P1

LAB829_H3
phyla|11v2|SRR099035X159
5795
10055
668
83.99
glotblastn

24_T1

LAB829_H8
monkeyflower|12v1|CV51881
5796
10056
668
82.6
globlastp

3_P1

LAB829_H4
amsonia|11v1|SRR098688X1
5797
10057
668
81.5
globlastp

02695_P1

LAB829_H5
orobanche|10v1|SRR023189S
5798
10058
668
80.4
globlastp

0012386_P1

LAB829_H6
tabernaemontana|11v1|SRR09
5799
10059
668
80.33
glotblastn

8689X121124

LAB830_H1
solanum_phureja|09v1|SPHA
5800
10060
669
96
globlastp

W615872

LAB830_H2
nicotiana_benthamiana|12v1|
5801
10061
669
89.7
globlastp

BP134056_P1

LAB830_H3
nicotiana_benthamiana|12v1|
5802
10062
669
88.7
globlastp

FG636634_P1

LAB831_H1
potato|10v1|BG589440_P1
5803
10063
670
97.1
globlastp

LAB831_H2
solanum_phureja|09v1|SPHB
5804
10064
670
96.6
globlastp

G125080

LAB831_H9
nicotiana_benthamiana|12v1|
5805
10065
670
93.8
globlastp

FS391047_P1

LAB831_H10
nicotiana_benthamiana|12v1|
5806
10066
670
93.3
globlastp

CN747454_P1

LAB831_H3
tobacco|gb162|CV018371
5807
10066
670
93.3
globlastp

LAB831_H4
eggplant|10v1|FS011292_P1
5808
10067
670
88.9
globlastp

LAB831_H11
pepper|12v1|DQ677335_P1
5809
10068
670
88.5
globlastp

LAB831_H6
tobacco|gb162|EB102915
5810
10069
670
86.1
globlastp

LAB831_H7
potato|10v1|BQ516860_P1
5811
10070
670
85.1
globlastp

LAB831_H8
solanum_phureja|09v1|SPHB
5812
10071
670
84.6
globlastp

Q516860

LAB832_H2
potato|10v1|BF153848_T1
5813
10072
671
87.17
glotblastn

LAB832_H1
solanum_phureja|09v1|SPHB
5814
10073
671
86.84
glotblastn

G125441

LAB832_H3
potato|10v1|BE922162_T1
5815
10074
671
86.52
glotblastn

LAB832_H5
potato|10v1|BE920375_T1
5816
10075
671
84.96
glotblastn

LAB833_H1
solanum_phureja|09v1|SPHB
5817
10076
672
94.6
globlastp

G126165

LAB833_H2
nicotiana_benthamiana|12v1|
5818
10077
672
83.7
globlastp

DV999481_P1

LAB833_H3
pepper|12v1|SRR203275X36
5819
10078
672
82
globlastp

332D1_P1

LAB834_H1
solanum_phureja|09v1|SPHB
5820
10079
673
92.6
globlastp

G128255

LAB834_H2
potato|10v1|BF053452_T1
5821
10080
673
83.62
glotblastn

LAB834_H3
eggplant|10v1|FS086065_T1
5822
10081
673
82.39
glotblastn

LAB835_H1
potato|10v1|BG597496_P1
5823
10082
674
97.9
globlastp

LAB835_H2
solanum_phureja|09v1|SPHB
5824
10082
674
97.9
globlastp

G128279

LAB835_H3
eggplant|10v1|FS015792_P1
5825
10083
674
92.8
globlastp

LAB835_H4
tobacco|gb162|AJ717989
5826
10084
674
90.8
globlastp

LAB835_H6
nicotiana_benthamiana|12v1|
5827
10085
674
90.3
globlastp

BP750228_P1

LAB835_H5
petunia|gb171|DY395411_P1
5828
10086
674
85.6
globlastp

LAB836_H1
potato|10v1|CK720403_P1
5829
10087
675
99.3
globlastp

LAB836_H2
solanum_phureja|09v1|SPHB
5830
10087
675
99.3
globlastp

G132326

LAB836_H3
eggplant|10v1|FS003483_P1
5831
10088
675
97.8
globlastp

LAB836_H12
pepper|12v1|BM062380_P1
5832
10089
675
96.3
globlastp

LAB836_H4
pepper|gb171|BM062380
5833
10089
675
96.3
globlastp

LAB836_H13
nicotiana_benthamiana|12v1|
5834
10090
675
92.6
globlastp

BP131040_P1

LAB836_H5
tobacco|gb162|BP192508
5835
10091
675
91.9
globlastp

LAB836_H14
nicotiana_benthamiana|12v1|
5836
10092
675
91.1
globlastp

EB429839_P1

LAB836_H6
petunia|gb171|FN008026_T1
5837
10093
675
89.71
glotblastn

LAB836_H7
ipomoea_nil|10v1|BJ574118_—
5838
10094
675
83.7
globlastp

P1

LAB836_H8
amsonia|11v1|SRR098688X1
5839
10095
675
82.2
globlastp

59842_P1

LAB836_H9
ipomoea_batatas|10v1|EE882
5840
10096
675
82.2
globlastp

586_P1

LAB836_H10
catharanthus|11v1|SRR09869
5841
10097
675
80.15
glotblastn

1X209596XX2_T1

LAB836_H11
castorbean|12v1|XM_002519
5842
10098
675
80
glotblastn

258_T1

LAB837_H1
potato|10v1|BF154003_P1
5843
10099
676
96
globlastp

LAB837_H2
solanum_phureja|09v1|SPHB
5844
10099
676
96
globlastp

G626094

LAB837_H5
pepper|12v1|CA518431_P1
5845
10100
676
90.8
globlastp

LAB837_H3
pepper|gb171|CA518431
5846
10100
676
90.8
globlastp

LAB837_H6
nicotiana_benthamiana|12v1|
5847
10101
676
89.6
globlastp

EB695326_P1

LAB837_H7
nicotiana_benthamiana|12v1|
5848
10102
676
88.5
globlastp

AF124370_P1

LAB837_H4
solanum_phureja|09v1|SPHB
5849
10103
676
81.7
globlastp

F187055

LAB839_H1
solanum_phureja|09v1|SPHB
5850
10104
677
92.6
globlastp

G629779

LAB839_H2
potato|10v1|BQ509981_P1
5851
10105
677
92.3
globlastp

LAB840_H1
solanum_phureja|09v1|SPHA
5852
10106
678
97.4
globlastp

W092358

LAB840_H2
potato|10v1|BQ515444_P1
5853
10107
678
91
globlastp

LAB840_H4
nicotiana_benthamiana|12v1|
5854
10108
678
88.4
globlastp

EB678287_P1

LAB840_H3
tobacco|gb162|EB678029
5855
10109
678
82.5
globlastp

LAB841_H1
solanum_phureja|09v1|SPHB
5856
10110
679
98.5
globlastp

G734916

LAB841_H3
pepper|12v1|CA521776_P1
5857
10111
679
92.6
globlastp

LAB841_H2
pepper|gb171|CA521776
5858
10111
679
92.6
globlastp

LAB841_H4
nicotiana_benthamiana|12v1|
5859
10112
679
88.7
globlastp

CK281783_P1

LAB842_H1
potato|10v1|BQ512667_P1
5860
10113
680
96.9
globlastp

LAB842_H2
solanum_phureja|09v1|SPHB
5861
10114
680
95.4
globlastp

Q512667

LAB842_H3
eggplant|10v1|FS052532_P1
5862
10115
680
90.8
globlastp

LAB842_H10
pepper|12v1|GD067875_P1
5863
10116
680
84.6
globlastp

LAB842_H11
nicotiana_benthamiana|12v1|
5864
10117
680
83.8
globlastp

EB695138_P1

LAB842_H12
nicotiana_benthamiana|12v1|
5865
10118
680
83.08
glotblastn

EB690970_T1

LAB842_H4
tobacco|gb162|EB432004
5866
10119
680
82.31
glotblastn

LAB842_H5
pepper|gb171|GD067875
5867
10120
680
81.5
globlastp

LAB842_H13
olea|13v1|SRR014463X38844
5868
10121
680
80.77
glotblastn

D1_T1

LAB842_H6
grape|11v1|GSVIVT0102846
5869
10122
680
80.77
glotblastn

2001_T1

LAB842_H7
olea|11v1|SRR014464.23049
5870
10123
680
80.77
glotblastn

LAB842_H8
antirrhinum|gb166|AJ559242
5871
10124
680
80
glotblastn

_T1

LAB842_H9
oak|10v1|FP050868_T1
5872
10125
680
80
glotblastn

LAB843_H1
solanum_phureja|09v1|SPHC
5873
10126
681
96
globlastp

O751611

LAB843_H2
solanum_phureja|09v1|SPHA
5874
10127
681
80.3
globlastp

M908029_P1

LAB844_H5
oat|11v1|GR358375_T1
5875
10128
682
85.78
glotblastn

LAB844_H6
rye|12v1|DRR001012.119017
5876
10129
682
85
globlastp

LAB844_H15
switchgrass|12v1|SRR187765
5877
10130
682
82.4
globlastp

.432740_P1

LAB845_H4
wheat|12v3|BF201349_T1
5878
10131
683
96.97
glotblastn

LAB845_H2
oat|11v1|CN815375_T1
5879
10132
683
86.25
glotblastn

LAB845_H3
brachypodium|12v1|BRADI5
5880
10133
683
85.7
globlastp

G24290_P1

LAB847_H1
rye|12v1|BE493992
5881
10134
684
96.5
globlastp

LAB847_H2
brachypodium|12v1|BRADI1
5882
10135
684
83.8
globlastp

G35477_P1

LAB850_H1
rye|12v1|DRR001012.107924
5883
10136
687
98.5
globlastp

LAB850_H6
sorghum|12v1|SB07G023430
5884
10137
687
81.1
globlastp

LAB621_H11
wheat|12v3|BE638019_T1
5885
10138
691
97.23
glotblastn

LAB621_H12
wheat|12v3|CD918539_T1
5886
10139
691
97.23
glotblastn

LAB621_H3
rye|12v1|DRR001012.108778
5887
10140
691
96.44
glotblastn

LAB621_H13
switchgrass|12v1|FL697470_—
5888
10141
691
83.4
globlastp

P1

LAB621_H5
foxtail_millet|11v3|PHY7SI0
5889
10142
691
83.4
glotblastn

05901M_T1

LAB621_H6
sorghum|12v1|SB10G000870
5890
10143
691
83.4
glotblastn

P1

LAB621_H7
switchgrass|gb167|FL697470
5891
10144
691
83.4
glotblastn

LAB621_H8
millet|10v1|EVO454PM0194
5892
10145
691
83
glotblastn

14_T1

LAB621_H7
switchgrass|12v1|FL802319_—
5893
10146
691
82.21
glotblastn

T1

LAB621_H9
maize|10v1|BG841651_T1
5894
10147
691
82.21
glotblastn

LAB621_H10
maize|10v1|AI586625_T1
5895
10148
691
81.82
glotblastn

LAB621_H14
switchgrass|12v1|GD006220_—
5896
10149
691
81.5
glotblastn

T1

LAB621_H15
switchgrass|12v1|FL821514_—
5897
10150
691
81.4
globlastp

P1

LAB624_H1
rye|12v1|DRR001012.115387
5898
10151
692
96.85
glotblastn

LAB624_H2
wheat|10v2|BE500006
5899
10152
692
96.85
glotblastn

LAB627_H1
rye|12v1|DRR001012.207497
5900
10153
693
99.38
glotblastn

LAB627_H6
switchgrass|gb167|FE614553
5901
10154
693
93.44
glotblastn

LAB627_H8
millet|10v1|EVO454PM0307
5902
10155
693
93.12
glotblastn

66_T1

LAB627_H10
rye|12v1|DRR001012.287479
5903
10156
693
88.8
globlastp

LAB627_H32
switchgrass|12v1|FE614553_—
5904
10157
693
86.9
globlastp

P1

LAB627_H13
orange|11v1|CK701542_T1
5905
10158
693
82.24
glotblastn

LAB627_H14
poppy|11v1|SRR030259.1504
5906
10159
693
82.24
glotblastn

82_T1

LAB627_H15
poppy|11v1|FE966907_T1
5907
10160
693
81.93
glotblastn

LYD577_H7
eucalyptus|11v2|CD668107_T
5908
10161
693
81.62
glotblastn

1

LYD577_H13
clementine|11v1|CK701542_—
5909
10162
693
81.62
glotblastn

T1

LAB627_H16
amsonia|11v1|SRR098688X1
5910
10163
693
81.56
glotblastn

03406_T1

LAB627_H17
solanum_phureja|09v1|SPHA
5911
10164
693
81.56
glotblastn

W160103

LAB627_H18
tomato|11v1|AW160103
5912
10165
693
81.56
glotblastn

LAB627_H19
beet|12v1|BQ584887_T1
5913
10166
693
81
glotblastn

LAB627_H20
artemisia|10v1|EY073341_T1
5914
10167
693
80.69
glotblastn

LAB627_H21
cirsium|11v1|SRR346952.115
5915
10168
693
80.69
glotblastn

416XX1_T1

LAB627_H22
euonymus|11v1|SRR070038X
5916
10169
693
80.69
glotblastn

198354_T1

LAB627_H23
silene|11v1|SRR096785X103
5917
10170
693
80.69
glotblastn

972

LYD577_H14
valeriana|11v1|SRR099039X1
5918
10171
693
80.62
glotblastn

10137

LAB627_H24
aristolochia|10v1|SRR039082
5919
10172
693
80.4
globlastp

S0042321_P1

LAB627_H25
euonymus|11v1|SRR070038X
5920
10173
693
80.37
glotblastn

108931_T1

LAB627_H26
sunflower|12v1|DY906340
5921
10174
693
80.37
glotblastn

LAB627_H27
thellungiella_parvulum|11v1|
5922
10175
693
80.37
glotblastn

BY808300

LYD577_H16
thellungiella_halophilum|11v
5923
10176
693
80.37
glotblastn

1|BY808300

LAB627_H28
ambrosia|11v1|SRR346935.1
5924
10177
693
80.06
glotblastn

0122_T1

LAB627_H29
fagopyrum|11v1|SRR063689
5925
10178
693
80.06
glotblastn

X204788_T1

LAB627_H30
tripterygium|11v1|SRR09867
5926
10179
693
80.06
glotblastn

7X106452

LYD577_H1
soybean|11v1|GLYMA04G39
5927
10180
693
80.06
glotblastn

980

LYD577_H1
soybean|12v1|GLYMA04G39
5928
10181
693
80.06
glotblastn

980_T1

LYD577_H5
apple|11v1|CN911043_T1
5929
10182
693
80.06
glotblastn

LAB627_H33
nicotiana_benthamiana|12v1|
5930
10183
693
80
glotblastn

EB691614_T1

LAB627_H31
vinca|11v1|SRR098690X1206
5931
10184
693
80
glotblastn

06

LAB641_H6
millet|10v1|EVO454PM0160
5932
10185
694
85.86
glotblastn

78_T1

LAB641_H22
wheat|12v3|CA677082_P1
5933
10186
694
84.8
globlastp

LAB641_H9
sugarcane|10v1|CA080518
5934
10187
694
82.2
globlastp

LAB641_H23
switchgrass|12v1|FL754120_—
5935
10188
694
81.44
glotblastn

T1

LAB641_H11
millet|10v1|EVO454PM0109
5936
10189
694
81.44
glotblastn

06_T1

LAB641_H24
switchgrass|12v1|FL710156_—
5937
10190
694
81.19
glotblastn

T1

LAB641_H12
sugarcane|10v1|CA078774
5938
10191
694
80.94
glotblastn

LAB641_H13
switchgrass|gb167|FL710156
5939
10192
694
80.94
glotblastn

LAB641_H16
barley|10v2|BG417194
5940
10193
694
80.15
glotblastn

LAB641_H16
barley|12v1|BG417194_T1
5941
10194
694
80.15
glotblastn

LAB644_H3
wheat|12v3|GH725267_T1
5942
10195
695
86.97
glotblastn

LAB644_H1
rye|12v1|DRR001012.45195
5943
10196
695
86.55
glotblastn

LAB644_H4
wheat|12v3|SRR400828X433
5944
10197
695
85.7
globlastp

857D1_P1

LAB644_H2
rye|12v1|BE637332
5945
10198
695
83.61
glotblastn

LAB645_H1
wheat|10v2|BE400082
5946
10199
696
85.31
glotblastn

LAB645_H1
wheat|12v3|BE400082_T1
5947
10200
696
85.31
glotblastn

LAB645_H2
rye|12v1|DRR001012.27223
5948
10201
696
83.41
glotblastn

LAB647_H1
rye|12v1|DRR001012.248065
5949
10202
697
97.75
glotblastn

LAB647_H2
brachypodium|12v1|BRADI2
5950
10203
697
91.01
glotblastn

G02320_T1

LAB647_H3
rice|11v1|BI805418
5951
10204
697
85.39
glotblastn

LAB647_H4
foxtail_millet|11v3|PHY7SI0
5952
10205
697
84.27
glotblastn

00630M_T1

LAB647_H5
millet|10v1|PMSLX0290525_—
5953
10206
697
84.27
glotblastn

T1

LAB647_H8
switchgrass|12v1|FE639638_—
5954
10207
697
83.15
glotblastn

T1

LAB647_H6
switchgrass|gb167|FE639637
5955
10208
697
83.15
glotblastn

LAB647_H6
switchgrass|12v1|FL746877_—
5956
10209
697
82.02
glotblastn

T1

LAB647_H7
sorghum|12v1|SB03G006950
5957
10210
697
82.02
glotblastn

LAB676_H1
sorghum|12v1|SB01G034220
5958
10211
699
89.6
globlastp

LAB676_H2
foxtail_millet|11v3|PHY7SI0
5959
10212
699
86.8
globlastp

36429M_P1

LAB676_H4
millet|10v1|EVO454PM0037
5960
10213
699
83.1
globlastp

38_P1

LAB711_H1
sugarcane|10v1|CA071910
5961
10214
700
88.26
glotblastn

LAB773_H1
maize|10v1|AI629496_P1
5962
10215
704
93.4
globlastp

LAB773_H2
foxtail_millet|11v3|PHY7SI0
5963
10216
704
88.6
globlastp

16120M_P1

LAB821_H1
ambrosia|11v1|SRR346935.1
5964
10217
707
93.9
globlastp

01779_P1

LAB821_H2
flaveria|11v1|SRR149232.116
5965
10218
707
85.47
glotblastn

790_T1

LAB821_H7
lettuce|12v1|DW045432_P1
5966
10219
707
84.5
globlastp

LAB821_H3
artemisia|10v1|EY110784_T1
5967
10220
707
83.72
glotblastn

LAB821_H4
cirsium|11v1|SRR346952.117
5968
10221
707
82.16
glotblastn

2833_T1

LAB821_H5
cirsium|11v1|SRR346952.221
5969
10222
707
82.16
glotblastn

629_T1

LAB821_H6
flaveria|11v1|SRR149229.280
5970
10223
707
81.5
globlastp

972_P1

LAB843_H3
nicotiana_benthamiana|12v1|
5971
10224
709
90
globlastp

BP748932_P1

LAB848, LAB
wheat|12v3|BE401010_P1
5972
10225
710
96.3
globlastp

848_H4, LAB8

48_H8

LAB848_H1
rye|12v1|DRR001012.562753
5973
10226
710
95.6
globlastp

LAB848_H2
rye|12v1|DRR001012.507278
5974
10227
710
94.9
globlastp

LAB848_H3
rye|12v1|DRR001016.182526
5975
10228
710
93.4
globlastp

LAB850_H8
barley|12v1|BE214655_T1
5976
10229
711
98.38
glotblastn

LAB850_H2
brachypodium|12v1|BRADI3
5977
10230
711
93.52
glotblastn

G12830T2_T1

LAB850_H3
oat|11v1|GR341709_T1
5978
10231
711
91.53
glotblastn

LAB850_H5
switchgrass|gb167|FL703371
5979
10232
711
89.47
glotblastn

LAB850_H9
switchgrass|12v1|FL703371_—
5980
10233
711
88.8
glotblastn

T1

LAB850_H7
maize|10v1|AI629577_T1
5981
10234
711
86
glotblastn

LAB619_H1
arabidopsis_lyrata|09v1|JGIA
5982
10235
712
96.5
globlastp

L024115_P1

LAB619_H2
thellungiella_parvulum|11v1|
5983
10236
712
89.8
globlastp

DN773103

LAB619_H3
thellungiella_halophilum|11v
5984
10237
712
88.7
globlastp

1|DN773103

LAB622_H1
rye|12v1|DRR001012.343588
5985
10238
713
98
globlastp

LAB622_H2
rye|12v1|DRR001012.34592
5986
10239
713
97.64
glotblastn

LAB622_H3
wheat|10v2|BE424934
5987
10240
713
97.2
globlastp

LAB622_H3
wheat|12v3|BE424934_P1
5988
10240
713
97.2
globlastp

LAB622_H4
pseudoroegneria|gb167|FF349
5989
10241
713
95.74
glotblastn

085

LAB622_H5
leymus|gb166|EG384339_P1
5990
10242
713
95.7
globlastp

LAB622_H6
oat|11v1|GO593206_P1
5991
10243
713
93.7
globlastp

LAB622_H7
brachypodium|12v1|BRADI5
5992
10244
713
88.9
globlastp

G01430_P1

LAB622_H9
rye|12v1|DRR001013.143989
5993
10245
713
83.33
glotblastn

LAB627_H34
wheat|12v3|BJ269172_P1
5994
10246
714
99.3
globlastp

LAB627_H2
wheat|10v2|BM136409
5995
10247
714
99.3
globlastp

LAB627_H3
brachypodium|12v1|BRADI1
5996
10248
714
96.4
globlastp

G76480_P1

LAB627_H4
sorghum|12v1|SB01G048480
5997
10249
714
92
globlastp

LAB627_H5
rice|11v1|AU068209
5998
10250
714
91.6
globlastp

LAB627_H9
foxtail_millet|11v3|PHY7SI0
5999
10251
714
91.2
globlastp

34117M_P1

LAB627_H7
maize|10v1|AI966896_P1
6000
10252
714
91.1
globlastp

LAB627_H11
oil_palm|11v1|EY396859_P1
6001
10253
714
84
globlastp

LAB627_H35
barley|12v1|BLYBJ_P1
6002
10254
714
83.3
globlastp

LAB627_H36
banana|12v1|MAGEN201200
6003
10255
714
81.5
globlastp

3843_P1

LYD577_H11
aquilegia|10v2|DR932473_P1
6004
10256
714
81
globlastp

LAB627_H12
phalaenopsis|11v1|SRR12577
6005
10257
714
80.9
globlastp

1.1005010_P1

LYD577_H4
grape|11v1|GSVIVT0102230
6006
10258
714
80.3
globlastp

0001_P1

LAB639_H6
wheat|12v3|CA606398_P1
6007
10259
716
93.8
globlastp

LAB639_H1
wheat|10v2|BE499292
6008
10260
716
92.48
glotblastn

LAB639_H2
rye|12v1|DRR001012.193437
6009
10261
716
90.85
glotblastn

LAB639_H3
brachypodium|12v1|BRADI2
6010
10262
716
84.3
globlastp

G28000_P1

LAB639_H5
rye|12v1|DRR001012.345503
6011
10263
716
83.3
globlastp

LAB651_H4
maize|10v1|AI861682_P1
6012
10264
720
82.9
globlastp

LAB656_H2
switchgrass|12v1|FL745052_—
6013
10265
721
91.8
globlastp

P1

LAB656_H1
maize|10v1|AW231879_P1
6014
10266
721
84.9
globlastp

LAB656_H3
maize|10v1|AI974917_T1
6015
10267
721
80.89
glotblastn

LAB680_H1
switchgrass|gb167|DN142749
6016
10268
725
96.6
globlastp

LAB680_H2
sugarcane|10v1|CA082081
6017
10269
725
96.3
globlastp

LAB680_H3
maize|10v1|AI973393_P1
6018
10270
725
96
globlastp

LAB680_H4
foxtail_millet|11v3|PHY7SI0
6019
10271
725
95.7
globlastp

17764M_P1

LAB680_H5
millet|10v1|EVO454PM0182
6020
10272
725
95.1
globlastp

61_P1

LAB680_H6
sorghum|12v1|SB04G029970
6021
10273
725
94.3
globlastp

LAB680_H8
brachypodium|12v1|BRADI3
6022
10274
725
90.5
globlastp

G53290_P1

LAB680_H9
cenchrus|gb166|EB652422_P
6023
10275
725
89.2
globlastp

1

LAB680_H10
leymus|gb166|EG375031_P1
6024
10276
725
87.5
globlastp

LAB680_H11
barley|10v2|BE411574
6025
10277
725
87.2
globlastp

LAB680_H12
wheat|10v2|BE428977
6026
10278
725
87
globlastp

LAB680_H12
wheat|12v3|BE637717_P1
6027
10278
725
87
globlastp

LAB680_H11
barley|12v1|BE411574_P1
6028
10279
725
86.9
globlastp

LAB680_H13
rye|12v1|DRR001012.106309
6029
10280
725
86.4
globlastp

LAB680_H19
switchgrass|12v1|DN142749_—
6030
10281
725
84.3
globlastp

P1

LAB680_H15
ginger|gb164|DY377432_P1
6031
10282
725
83.1
globlastp

LAB680_H20
banana|12v1|MAGEN201201
6032
10283
725
82.8
globlastp

2892_P1

LAB680_H21
switchgrass|12v1|FE603214_—
6033
10284
725
80.92
glotblastn

T1

LAB681_H18
banana|12v1|MAGEN201203
6034
10285
726
80
globlastp

3734_P1

LAB691_H1
sorghum|12v1|SB08G016990
6035
10286
730
87.9
globlastp

LAB691_H2
sugarcane|10v1|CA078344
6036
10287
730
87.1
globlastp

LAB707_H1
sorghum|12v1|SB03G029230
6037
10288
735
96.8
globlastp

LAB707_H2
foxtail_millet|11v3|PHY7SI0
6038
10289
735
88.4
globlastp

01649M_P1

LAB707_H4
rice|11v1|CF277431
6039
10290
735
83.5
globlastp

LAB710_H1
sorghum|12v1|SB06G017090
6040
10291
736
87.6
globlastp

LAB713_H2
sorghum|12v1|SB02G041680
6041
10292
737
82.6
globlastp

LAB727_H11
switchgrass|12v1|FL978719_—
6042
10293
741
87.4
globlastp

P1

LAB727_H3
wheat|10v2|CA729536
6043
10294
741
85.2
globlastp

LAB727_H4
millet|10v1|EVO454PM0299
6044
10295
741
85
globlastp

44_P1

LAB727_H5
brachypodium|12v1|BRADI2
6045
10296
741
84.8
globlastp

G62280_P1

LAB727_H6
sorghum|12v1|SB03G047400
6046
10297
741
84.5
globlastp

LAB727_H7
wheat|10v2|BE415712
6047
10298
741
84.4
globlastp

LAB727_H7
wheat|12v3|BE415712_P1
6048
10298
741
84.4
globlastp

LAB727_H8
barley|10v2|BF623281
6049
10299
741
84.1
globlastp

LAB727_H8
barley|12v1|BF623281_P1
6050
10299
741
84.1
globlastp

LAB727_H9
rye|12v1|DRR001012.128753
6051
10300
741
84.1
globlastp

LAB727_H10
rye|12v1|DRR001012.206796
6052
10301
741
82.1
globlastp

LAB736_H46
cowpea|12v1|FF383571_P1
6053
10302
743
85.2
globlastp

6

LAB736_H46
flaveria|11v1|SRR149229.136
6054
10303
743
83.5
globlastp

7
186_P1

LAB736_H46
potato|10v1|AJ489132_P1
6055
10304
743
82
globlastp

8

LAB736_H46
tomato|11v1|BG131735_P1
6056
10304
743
82
globlastp

9

LAB736_H47
pepper|12v1|CA516335_P1
6057
10305
743
80.5
globlastp

0

LAB736_H47
marchantia|gb166|AU081872
6058
10306
743
80.3
globlastp

1
_P1

LAB742_H1
maize|10v1|AI947648_P1
6059
10307
744
88.1
globlastp

LAB742_H2
maize|10v1|BG320342_T1
6060
10308
744
85.27
glotblastn

LAB742_H4
switchgrass|12v1|FL874193_—
6061
10309
744
82.1
globlastp

P1

LAB742_H3
foxtail_millet|11v3|PHY7SI0
6062
10310
744
81.6
globlastp

09776M_P1

LAB749_H1
maize|10v1|BI361262_P1
6063
10311
745
84.7
globlastp

LAB749_H3
switchgrass|12v1|SRR187766
6064
10312
745
81.7
globlastp

.246857_P1

LAB750_H1
foxtail_millet|11v3|PHY7SI0
6065
10313
746
88
globlastp

36012M_P1

LAB750_H2
maize|10v1|AI861360_P1
6066
10314
746
87.5
globlastp

LAB750_H5
switchgrass|12v1|FE618981_—
6067
10315
746
87.2
globlastp

P1

LAB750_H4
foxtail_millet|11v3|PHY7SI0
6068
10316
746
83
globlastp

35943M_P1

LAB750_H5
switchgrass|gb167|FE618981
6069
10317
746
82.8
globlastp

LAB750_H6
brachypodium|12v1|BRADI3
6070
10318
746
82.3
globlastp

G20810_P1

LAB750_H7
pseudoroegneria|gb167|FF342
6071
10319
746
81.8
globlastp

468

LAB750_H8
barley|10v2|AV833391
6072
10320
746
81.5
globlastp

LAB750_H9
sorghum|12v1|SB01G025580
6073
10321
746
81.2
globlastp

LAB750_H13,
wheat|12v3|BQ578674_P1
6074
10322
746
81.2
globlastp

LAB750_H14

LAB750_H18
wheat|12v3|CA610088_P1
6075
10323
746
81
globlastp

LAB750_H10
rye|12v1|BE586383
6076
10324
746
81
globlastp

LAB750_H11
rye|12v1|DRR001012.193313
6077
10325
746
80.75
glotblastn

LAB750_H19
wheat|12v3|CA598769_P1
6078
10326
746
80.5
globlastp

LAB750_H12
rye|12v1|DRR001014.529135
6079
10327
746
80.5
globlastp

LAB750_H13
wheat|10v2|BG908748XX2
6080
10328
746
80.5
globlastp

LAB750_H14
wheat|10v2|BQ578674
6081
10329
746
80.5
globlastp

LAB750_H15
rice|11v1|U38132
6082
10330
746
80.4
globlastp

LAB750_H16
leymus|gb166|EG394412_P1
6083
10331
746
80.2
globlastp

LAB750_H17
rye|12v1|DRR001014.492971
6084
10332
746
80.2
globlastp

LAB751_H1
foxtail_millet|11v3|PHY7SI0
6085
10333
747
86.9
globlastp

35965M_P1

LAB751_H2
foxtail_millet|11v3|SICRP057
6086
10333
747
86.9
globlastp

625_P1

LAB751_H3
maize|10v1|CD439407_P1
6087
10334
747
85.2
globlastp

LAB751_H4
switchgrass|12v1|FE619135_—
6088
10335
747
80
globlastp

P1

LAB753_H1
maize|10v1|AW927437_P1
6089
10336
748
84.3
globlastp

LAB754_H1
maize|10v1|CX129591_T1
6090
10337
749
86.3
glotblastn

LAB757_H2
foxtail_millet|11v3|PHY7SI0
6091
10338
750
88
globlastp

30463M_P1

LAB757_H3
rice|11v1|CI075004
6092
10339
750
85.3
globlastp

LAB757_H5
barley|12v1|BQ471173_P1
6093
10340
750
82
globlastp

LAB764_H23
barley|12v1|BG368747_P1
6094
10341
751
84.3
globlastp

LAB764_H18
wheat|12v3|BQ903096_P1
6095
10342
751
83.4
globlastp

LAB764_H18
wheat|10v2|BQ903096
6096
10343
751
80.28
glotblastn

LAB789_H1
maize|10v1|AW061708_P1
6097
10344
752
95.8
globlastp

LAB789_H2
foxtail_millet|11v3|PHY7SI0
6098
10345
752
90.7
globlastp

09435M_P1

LAB789_H3
brachypodium|12v1|BRADI4
6099
10346
752
85.1
globlastp

G41137_P1

LAB789_H5
wheat|12v3|BE400988_P1
6100
10347
752
83.6
globlastp

LAB789_H4
rice|11v1|BM421752
6101
10348
752
83.51
glotblastn

LAB790_H1
sugarcane|10v1|CA275434
6102
10349
753
82
globlastp

LAB809_H2
pigeonpea|11v1|SRR054580X
6103
10350
754
88.4
globlastp

171437_P1

LAB809_H5
bean|12v2|FE898175_P1
6104
10351
754
85.8
globlastp

LAB809_H3
bean|12v1|FE898175
6105
10351
754
85.8
globlastp

LAB809_H4
pigeonpea|11v1|SRR054580X
6106
10352
754
84.1
globlastp

112350_P1

LAB809_H6
medicago|12v1|AW288006_T
6107
10353
754
80.09
glotblastn

1

LAB813_H1
sunflower|12v1|CF084564
6108
—
755
98.46
glotblastn

LAB813_H5
flaveria|11v1|SRR149239.619
6109
10354
755
85.5
globlastp

3_P1

LAB813_H9
ambrosia|11v1|SRR346943.1
6110
10355
755
81.2
glotblastn

03968_T1

LAB813_H10
lettuce|10v1|DW057004
6111
10356
755
80.5
globlastp

LAB814_H1
ambrosia|11v1|SRR346935.1
6112
10357
756
96.3
globlastp

47078_P1

LAB814_H2
flaveria|11v1|SRR149229.104
6113
10358
756
92
globlastp

448_P1

LAB814_H3
cirsium|11v1|SRR346952.135
6114
10359
756
86.9
globlastp

881XX1_P1

LAB814_H4
cirsium|11v1|SRR346952.100
6115
10360
756
86.7
globlastp

8592_P1

LAB814_H5
centaurea|gb166|EH721858_P
6116
10361
756
86.4
globlastp

1

LAB814_H6
arnica|11v1|SRR099034X139
6117
10362
756
85.5
globlastp

53_P1

LAB814_H8
artemisia|10v1|EY087660_P1
6118
10363
756
85.3
globlastp

LAB814_H7
ambrosia|11v1|SRR346935.3
6119
10364
756
85.06
glotblastn

5133_T1

LAB814_H9
sunflower|12v1|DY904337
6120
10365
756
81.29
glotblastn

LAB814_H10
cichorium|gb171|EH673207_—
6121
10366
756
80.76
glotblastn

T1

LAB814_H11
flaveria|11v1|SRR149229.114
6122
10367
756
80.53
glotblastn

421_T1

LAB814_H12
flaveria|11v1|SRR149229.334
6123
10368
756
80.35
glotblastn

922_T1

LAB816_H1
sunflower|12v1|EE616901
6124
10369
757
99.3
globlastp

LAB816_H2
sunflower|12v1|EL413033
6125
10370
757
95.6
globlastp

LAB816_H3
ambrosia|11v1|SRR346935.1
6126
10371
757
86.54
glotblastn

54181_T1

LAB816_H4
ambrosia|11v1|SRR346935.1
6127
10372
757
85.15
glotblastn

33162_T1

LAB816_H6
flaveria|11v1|SRR149232.324
6128
10373
757
84.5
globlastp

073_P1

LAB816_H5
ambrosia|11v1|SRR346935.1
6129
10374
757
84.49
glotblastn

86086_T1

LAB816_H7
ambrosia|11v1|SRR346935.1
6130
10375
757
80.65
glotblastn

5882_T1

LAB823_H1
ambrosia|11v1|SRR346935.1
6131
10376
760
84.13
glotblastn

11608_T1

LAB832_H4
solanum_phureja|09v1|SPHB
6132
10377
762
87.5
globlastp

G132941

LAB832_H6
eggplant|10v1|FS008181_P1
6133
10378
762
85.3
globlastp

LAB832_H7
eggplant|10v1|FS031703_P1
6134
10379
762
84.5
globlastp

LAB832_H8
tobacco|gb162|AJ632902_P1
6135
10380
762
81.4
globlastp

LAB837_H8
blueberry|12v1|SRR353282X
6136
10381
763
80.7
globlastp

2808D1_P1

LAB844_H1
rye|12v1|DRR001012.234482
6137
10382
765
97.3
globlastp

LAB844_H2
barley|10v2|BI951883
6138
10383
765
95.6
globlastp

LAB844_H2
barley|12v1|BI946897_P1
6139
10383
765
95.6
globlastp

LAB844_H3
rye|12v1|BE586821
6140
10384
765
91.1
globlastp

LAB844_H4
pseudoroegneria|gb167|FF364
6141
10385
765
86.7
globlastp

452

LAB844_H8
barley|10v2|BI947264
6142
10386
765
84.9
globlastp

LAB844_H16
barley|12v1|BI947264_P1
6143
10387
765
84.4
globlastp

LAB844_H7
millet|10v1|CD726687_P1
6144
10388
765
84.1
globlastp

LAB844_H10
wheat|12v3|BE401102_P1
6145
10389
765
84.1
globlastp

LAB844_H9
wheat|10v2|BE401102
6146
10390
765
83.6
globlastp

LAB844_H10
wheat|10v2|BG908694
6147
10391
765
82.74
glotblastn

LAB844_H12
sorghum|12v1|SB01G018490
6148
10392
765
82.3
globlastp

LAB844_H13
brachypodium|12v1|BRADI3
6149
10393
765
81.3
globlastp

G29710_P1

LAB844_H14
maize|10v1|AW355832_P1
6150
10394
765
80.7
globlastp

LAB844_H11
foxtail_millet|11v3|PHY7SI0
6151
10395
765
80.2
globlastp

39939M_P1

LAB845_H1
barley|10v2|BE194359
6152
10396
766
92.8
globlastp

LAB851, LAB
barley|12v1|BE194359_P1
6153
10397
766
92.8
globlastp

845_H1

LAB852
barley|12v1|AV836427_P1
6154
10398
767
95.4
globlastp

LAB848_H4
wheat|10v2|BE446148
6155
10399
768
94
globlastp

LAB848_H5
barley|10v2|BI954458
6156
10400
768
91
globlastp

LAB848_H6
barley|10v2|BF258149
6157
10401
768
90.4
globlastp

LAB848_H7
rye|12v1|DRR001012.120131
6158
10402
768
87.6
globlastp

LAB848_H8
wheat|10v2|BE401010
6159
10403
768
86.6
globlastp

LAB848_H9
oat|11v1|CN815872_P1
6160
10404
768
85.4
globlastp

LAB848_H10
oat|11v1|CN817079_P1
6161
10405
768
84.7
globlastp

LAB848_H11
brachypodium|12v1|BRADI3
6162
10406
768
83.2
globlastp

G42650_P1

LAB848_H12
rice|11v1|AA749628_T1
6163
—
768
80.74
glotblastn

LAB849
wheat|12v3|BG604521_P1
6164
10407
769
98.7
globlastp

LAB849_H9
wheat|12v3|HX128100_P1
6165
10408
769
97
globlastp

LAB849_H1
barley|12v1|BJ478174_P1
6166
10409
769
95.4
globlastp

LAB849_H1
barley|10v2|BJ478174
6167
10410
769
94.7
globlastp

LAB849_H2
leymus|gb166|EG380222_P1
6168
10411
769
94.4
globlastp

LAB849_H3
brachypodium|12v1|BRADI1
6169
10412
769
89.1
globlastp

G52180_P1

LAB849_H4
foxtail_millet|11v3|PHY7SI0
6170
10413
769
86.4
globlastp

06989M_P1

LAB849_H5
rice|11v1|CK034673
6171
10414
769
86.4
globlastp

LAB849_H6
sorghum|12v1|SB10G000450
6172
10415
769
85.1
globlastp

LAB849_H7
switchgrass|12v1|FL717341_—
6173
10416
769
84.8
globlastp

P1

LAB849_H7
switchgrass|gb167|FL717341
6174
10417
769
84.77
glotblastn

LAB849_H8
switchgrass|gb167|FE621912
6175
10418
769
84.44
glotblastn

LAB849_H8
switchgrass|12v1|FE621912_—
6176
10419
769
84.4
globlastp

P1

LAB850_H5
switchgrass|12v1|FE656940_—
6177
10420
770
86.6
globlastp

P1

LAB850_H4
foxtail_millet|11v3|PHY7SI0
6178
10421
770
85.3
globlastp

13129M_P1

Table 152: Provided are the homologous polypeptides (polyp.) and polynucleotides (polyn.) of the genes for increasing abiotic stress tolerance, yield, growth rate, vigor, oil content, fiber yield, fiber quality, biomass, nitrogen use efficiency, water use efficiency and fertilizer use efficiency genes of a plant which are listed in Table 151 above. Homology was calculated as % of identity over the aligned sequences. The query sequences were polynucleotide and polypeptides depicted in Table 151 above, and the subject sequences are protein and polynucleotide sequences identified in the database based on greater than 80% global identity to the query nucleotide and/or polypeptide sequences. Hom. = Homology; Glob. = Global; Algor. = Algorithm.

The output of the functional genomics approach described herein is a set of genes highly predicted to improve AB ST, yield and/or other agronomic important traits such as growth rate, vigor, biomass, growth rate, oil content, nitrogen use efficiency, water use efficiency and fertilizer use efficiency of a plant by increasing their expression. Although each gene is predicted to have its own impact, modifying the mode of expression of more than one gene is expected to provide an additive or synergistic effect on the plant yield and/or other agronomic important yields performance. Altering the expression of each gene described here alone or set of genes together increases the overall yield and/or other agronomic important traits, hence expects to increase agricultural productivity.

Example 20
Gene Cloning and Generation of Binary Vectors for Plant Expression

To validate their role in improving yield, selected genes were over-expressed in plants, as follows.

Cloning Strategy

Selected genes from those presented in Examples 1-19 hereinabove were cloned into binary vectors for the generation of transgenic plants. For cloning, the full-length open reading frames (ORFs) were identified. EST clusters and in some cases mRNA sequences were analyzed to identify the entire open reading frame by comparing the results of several translation algorithms to known proteins from other plant species.

In order to clone the full-length cDNAs, reverse transcription (RT) followed by polymerase chain reaction (PCR; RT-PCR) was performed on total RNA extracted from leaves, roots or other plant tissues, growing under normal/limiting or stress conditions. Total RNA extraction, production of cDNA and PCR amplification was performed using standard protocols described elsewhere (Sambrook J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning. A Laboratory Manual, 2nd Ed. Cold Spring Harbor Laboratory Press, New York.) which are well known to those skilled in the art. PCR products were purified using PCR purification kit (Qiagen).

Usually, 2 sets of primers were prepared for the amplification of each gene, via nested PCR (if required). Both sets of primers were used for amplification on cDNA. In case no product was obtained, a nested PCR reaction was performed. Nested PCR was performed by amplification of the gene using external primers and then using the produced PCR product as a template for a second PCR reaction, where the internal set of primers were used. Alternatively, one or two of the internal primers were used for gene amplification, both in the first and the second PCR reactions (meaning only 2-3 primers are designed for a gene). To facilitate further cloning of the cDNAs, an 8-12 base pairs (bp) extension was added to the 5′ of each internal primer. The primer extension includes an endonuclease restriction site. The restriction sites were selected using two parameters: (a) the restriction site does not exist in the cDNA sequence; and (b) the restriction sites in the forward and reverse primers were designed such that the digested cDNA was inserted in the sense direction into the binary vector utilized for transformation.

PCR products were digested with the restriction endonucleases (New England BioLabs Inc) according to the sites designed in the primers. Each digested PCR product was inserted into a high copy vector pUC19 (New England BioLabs Inc], or into plasmids originating from this vector. In some cases the undigested PCR product was inserted into pCR-Blunt II-TOPO (Invitrogen) or into pJET1.2 (CloneJET PCR Cloning Kit, Thermo Scientific) or directly into the binary vector. The digested products and the linearized plasmid vector were ligated using T4 DNA ligase enzyme (Roche, Switzerland).

Sequencing of the inserted genes was performed, using the ABI 377 sequencer (Applied Biosystems). In some cases, after confirming the sequences of the cloned genes, the cloned cDNA was introduced into a modified pGI binary vector containing the At6669 promoter (e.g., pQFNc) and the NOS terminator (SEQ ID NO: 10457) via digestion with appropriate restriction endonucleases.

Several DNA sequences of the selected genes were synthesized by GeneArt (Life Technologies, Grand Island, N.Y., USA). Synthetic DNA was designed in silico. Suitable restriction enzymes sites were added to the cloned sequences at the 5′ end and at the 3′ end to enable later cloning into the desired binary vector.

Binary vectors—The pPI plasmid vector was constructed by inserting a synthetic poly-(A) signal sequence, originating from pGL3 basic plasmid vector (Promega, GenBank Accession No. U47295; nucleotides 4658-4811) into the HindIII restriction site of the binary vector pBI101.3 (Clontech, GenBank Accession No. U12640). pGI is similar to pPI, but the original gene in the backbone is GUS-Intron and not GUS.

The modified pGI vector (e.g., pQFN, pQFNc, pQYN_—6669, pQNa_RP, pQFYN or pQXNc) is a modified version of the pGI vector in which the cassette is inverted between the left and right borders so the gene and its corresponding promoter are close to the right border and the NPTII gene is close to the left border.

At6669, the new Arabidopsis thaliana promoter sequence (SEQ ID NO:10446) was inserted in the modified pGI binary vector, upstream to the cloned genes, followed by DNA ligation and binary plasmid extraction from positive E. coli colonies, as described above. Colonies were analyzed by PCR using the primers covering the insert which were designed to span the introduced promoter and gene. Positive plasmids were identified, isolated and sequenced.

In case genomic DNA was cloned, the genes were amplified by direct PCR on genomic DNA extracted from leaf tissue using the DNAeasy kit (Qiagen Cat. No. 69104).

Selected genes cloned by the present inventors are provided in Table 153 below.

TABLE 153

Gene

Polynucleotide
Polypeptide

Name
High copy plasmid
Organism
Primers used SEQ ID NOs:
SEQ ID NO:
SEQ ID NO:

LAB616
pUC19c_LAB616

arabidopsis

11010, 11055, 10978, 11062
263
475

LAB617
pQFNc_LAB617

arabidopsis

10583, 10862, 10583, 10862
264
476

LAB618
pMA-T_LAB618_GA

arabidopsis

265
477

LAB619
pQFNc_LAB619

arabidopsis

10780, 10874, 10691, 10876
266
712

LAB620
pUC19c_LAB620
barley
10721, 10833, 10709, 10872
267
479

LAB622
pQFNc_LAB622
barley
11002, 10537, 11002, 10537
268
713

LAB623
pUC19c_LAB623
barley
10642, 10940, 10643, 10948
269
482

LAB624
pQFNc_LAB624
barley
10706, 10881, 10699, 10917
270
483

LAB625
pUC19c_LAB625
barley
11001, 10501, 11001, 10501
271
484

LAB626
pQFNc_LAB626
barley
11017, 10550, 11017, 10550
272
485

LAB627
pUC19c_LAB627
barley
10483, 11065, 10483, 11065
273
714

LAB628
pUC19_LAB628
barley
10977, 10539, 11025, 10535
274
487

LAB629
pUC19c_LAB629
barley
11030, 10458, 11011, 10465
275
488

LAB630
pMA-RQ_LAB630_GA
barley

276
489

LAB631
pQFNc_LAB631
barley
11003, 10526, 11007, 10531
277
490

LAB632
pQFNc_LAB632
barley
10686, 10858, 10632, 10866
278
491

LAB633
pQFNc_LAB633
barley
10646, 10893, 10646, 10893
279
492

LAB634
pUC19c_LAB634
barley
10594, 10813, 10594, 10813
280
493

LAB635
TopoB_LAB635
barley
10994, 10534, 11005, 10534
281
715

LAB636
pUC19c_LAB636
barley
10983, 10795, 10991, 10961
282
495

LAB637
pUC19c_LAB637
barley
10741, 10935, 10741, 10935
283
496

LAB638
pUC19c_LAB638
barley
10694, 11080, 10694, 11080
284
497

LAB639
pUC19c_LAB639
barley
10753, 10942, 10667, 10789
285
716

LAB640
pQFNc_LAB640
barley
10484, 10517, 10484, 10517
286
499

LAB641
TopoB_LAB641
barley
11009, 10564, 11038, 10560
287
500

LAB642
pUC19c_LAB642
barley
10522, 10474, 10515, 10480
288
717

LAB645
pQFNc_LAB645
barley
10558, 11056, 10558, 11056
289
718

LAB647
pMA_LAB647_GA
barley

290
504

LAB649
pQFNc_LAB649
canola
11018, 10538, 11018, 10538
291
505

LAB650
pUC19c_LAB650
cotton
10782, 10915, 10598, 10834
292
719

LAB651
pUC19c_LAB651
foxtail_millet
10972, 10460, 10972, 10460
293
720

LAB652
pQFNc_LAB652
foxtail_millet
10688, 10939, 10739, 10934
294
508

LAB653
pUC19c_LAB653
foxtail_millet
10700, 10528, 10700, 10528
295
509

LAB654
pUC19c_LAB654
foxtail_millet
10735, 11084, 10670, 11083
296
510

LAB655
pUC19c_LAB655
foxtail_millet
10714, 10845, 11044, 10788
297
511

LAB656
pQFNc_LAB656
foxtail_millet
10740, 10836, 10740, 10836
298
721

LAB657
pQFNc_LAB657
foxtail_millet
10690, 10849, 10593, 10873
299
513

LAB659
pQFNc_LAB659
foxtail_millet
10703, 10949, 10681, 10906
300
514

LAB660
pUC19c_LAB660
foxtail_millet
11050, 10812, 10719, 10884
301
515

LAB661
pUC19c_LAB661
foxtail_millet
10997, 10595, 10963, 10581
302
516

LAB662
pQFNc_LAB662
foxtail_millet
10713, 10901, 10713, 10891
303
517

LAB663
pUC19_LAB663
foxtail_millet
10498, 10556, 10485, 10548
304
518

LAB664
pQFNc_LAB664
foxtail_millet
10623, 10796, 10623, 10925
305
519

LAB665
pQFNc_LAB665
foxtail_millet
10968, 10678, 10967, 10674
306
520

LAB666
pQFNc_LAB666
foxtail_millet
11032, 10608, 11008, 10738
307
521

LAB667
pUC19c_LAB667
foxtail_millet
10981, 10756, 10981, 10756
308
522

LAB668
pQFNc_LAB668
foxtail_millet
11037, 10462, 11092, 10472
309
523

LAB669_H7
pMA-RQ_LAB669_H7_GA
rice

470
688

LAB670
pQFNc_LAB670
foxtail_millet
10654, 10883, 10635, 10931
310
525

LAB671
pUC19c_LAB671
foxtail_millet
11021, 10640, 11021, 10640
311
526

LAB672
pUC19c_LAB672
gossypium_raimondii
10995, 10459, 11016, 10467
312
722

LAB673
pQFNc_LAB673
maize
10591, 10829, 10591, 10829
313
723

LAB675
pQFNc_LAB675
maize
10503, 11061, 10544, 11042
314
529

LAB676
pMA-T_LAB676_GA
maize

315
530

LAB677
pQFNc_LAB677
maize
10494, 10466, 10493, 10473
316
724

LAB678
pMA-RQ_LAB678_GA
maize

317
532

LAB679
pQFNc_LAB679
maize
10572, 10547, 10572, 10547
318
533

LAB680
pQFNc_LAB680
maize
11013, 11067, 11013, 11067
319
725

LAB681
pUC19_LAB681
maize
10701, 10842, 10701, 10842
320
726

LAB682
TopoB_LAB682
maize
10971, 10570, 10971, 10565
321
727

LAB683
pQFNc_LAB683
maize
10610, 10800, 10758, 10912
322
537

LAB684
pUC19c_LAB684
maize
10614, 11047, 11051, 10807
323
538

LAB685
pMA-RQ_LAB685_GA
maize

324
539

LAB686
pUC19d_LAB686
maize
10974, 10573, 10990, 10574
325
728

LAB687
pQFNc_LAB687
maize
11045, 10897, 10661, 10863
326
541

LAB688
pQFNc_LAB688
maize
10553, 11064, 10553, 11064
327
729

LAB689
pQFNc_LAB689
maize
10668, 10817, 10600, 10932
328
543

LAB690
pMA-RQ_LAB690_GA
maize

329
544

LAB691
pQFNc_LAB691
maize
10621, 10952, 11043, 11052
330
730

LAB692
pUC19c_LAB692
maize
10586, 10848, 10586, 10848
331
546

LAB693
pQFNc_LAB693
maize
10757, 10919, 10720, 10841
332
547

LAB694
pQFNc_LAB694
maize
10712, 10885, 10606, 10910
333
548

LAB695
pQFNc_LAB695
maize
10618, 10823, 10648, 10889
334
549

LAB696
pUC19c_LAB696
maize
10984, 10476, 11015, 10479
335
550

LAB697
pQFNc_LAB697
maize
10630, 10888, 10616, 10854
336
551

LAB698
TopoB_LAB698
maize
11033, 10748, 11033, 10765
337
731

LAB700
TopoB_LAB700
maize
10609, 10951, 10734, 10846
338
732

LAB701
pUC19c_LAB701
maize
10659, 10859, 10620, 10922
339
554

LAB702
pMA-RQ_LAB702_GA
maize

340
555

LAB703
pUC19c_LAB703
maize
10577, 10832, 10592, 10843
341
733

LAB704
pUC19c_LAB704
maize
10781, 10916, 10677, 10824
342
557

LAB705
pQFNc_LAB705
maize
10987, 10563, 10987, 10519
343
734

LAB706
pQFNc_LAB706
maize
10736, 10929, 10578, 10895
344
559

LAB707
pUC19c_LAB707
maize
10965, 10554, 10970, 10506
345
735

LAB708
pUC19c_LAB708
maize
10551, 10477, 10551, 10477
346
561

LAB709
pUC19_LAB709
maize
10727, 10827, 10687, 10875
347
562

LAB710
pQFNc_LAB710
maize
10976, 10742, 10976, 10742
348
736

LAB711
pUC19c_LAB711
maize
10724, 10937, 10726, 11090
349
564

LAB712
pUC19c_LAB712
maize
11004, 10549, 10979, 10541
350
565

LAB713
pQFNc_LAB713
maize
10530, 11059, 10530, 11059
351
737

LAB714
pUC19c_LAB714
maize
10613, 10899, 10658, 10867
352
567

LAB716
pMA-T_LAB716_GA
poplar

353
568

LAB717
pMA-RQ_LAB717_GA
poplar

354
569

LAB718
pMA-RQ_LAB718_GA
poplar

355
570

LAB719
pQFNc_LAB719
rice
10680, 10902, 10666, 10831
356
571

LAB721
pUC19c_LAB721
rice
10973, 10559, 11026, 10540
357
738

LAB722
pUC19c_LAB722
rice
10486, 10513, 10486, 10513
358
739

LAB723
pMA-T_LAB723_GA
rice

359
575

LAB724
pUC19c_LAB724
rice
10619, 10892, 10619, 10930
360
576

LAB725
pUC19c_LAB725
rice
11020, 10612, 11040, 10672
361
740

LAB726
pQFNc_LAB726
rice
10641, 10927, 10603, 10953
362
578

LAB727
pQFNc_LAB727
rice
10496, 10524, 10496, 10524
363
741

LAB728
pUC19c_LAB728
rice
11034, 10746, 11027, 10584
364
580

LAB729
pQFNc_LAB729
rice
11041, 11058, 10998, 11057
365
742

LAB730
pUC19c_LAB730
rice
10745, 10908, 10750, 10880
366
582

LAB731
pUC19c_LAB731
rice
11036, 10557, 11036, 10557
367
583

LAB732
pQFNc_LAB732
rice
10512, 10469, 10546, 10463
368
584

LAB733
pUC19c_LAB733
rice
10692, 10959, 10647, 10814
369
585

LAB734
pMA-RQ_LAB734_GA
rice

370
586

LAB735
pQFNc_LAB735
rice
10500, 11071, 10500, 11071
371
587

LAB736
pQFNc_LAB736
rice
10492, 10552, 10492, 10552
372
743

LAB738
pUC19c_LAB738
rice
10771, 10837, 10749, 10815
373
589

LAB739
pUC19_LAB739
rice
10760, 10805, 10760, 10805
374
590

LAB740
pUC19c_LAB740

sorghum

10665, 11066, 10779, 11073
375
591

LAB741
pUC19_LAB741

sorghum

11000, 11053, 11000, 11053
376
592

LAB742
pUC19c_LAB742

sorghum

10705, 10533, 10705, 10533
377
744

LAB744_H1
pMA-RQ_LAB744_H1_GA
maize

471
689

LAB745
pQFNc_LAB745p

sorghum

10986, 11072, 10986, 11072
473
—

LAB746
pUC19_LAB746

sorghum

10585, 10962, 10585, 10962
378
595

LAB747
pQFNc_LAB747

sorghum

10980, 10523, 10980, 10523
474
—

LAB748
pQFNc_LAB748

sorghum

10722, 10921, 10722, 10946
379
596

LAB749
pQFNc_LAB749

sorghum

10571, 10529, 10571, 10529
380
745

LAB750
pUC19c_LAB750

sorghum

10491, 10536, 10491, 10536
381
746

LAB751
pUC19_LAB751

sorghum

10982, 10509, 10982, 10509
382
747

LAB752
pUC19c_LAB752

sorghum

10652, 10525, 10693, 10521
383
600

LAB753
pUC19c_LAB753

sorghum

10644, 10804, 10639, 10907
384
748

LAB754
TopoB_LAB754

sorghum

10587, 10938, 10707, 10793
385
749

LAB755
pUC19c_LAB755

sorghum

10684, 10809, 10683, 10943
386
603

LAB756_H3
pMA-RQ_LAB756_H3_GA
rice

472
690

LAB757
pQFNc_LAB757

sorghum

10673, 10818, 10673, 10818
387
750

LAB758
TopoB_LAB758

sorghum

10964, 10543, 11039, 10543
388
606

LAB759
pQFNc_LAB759

sorghum

10725, 10944, 10766, 10882
389
607

LAB760
pUC19c_LAB760

sorghum

10645, 10924, 10611, 10816
390
608

LAB761
pMA-RQ_LAB761_GA

sorghum

391
609

LAB762
pUC19c_LAB762

sorghum

10489, 10514, 10495, 10542
392
610

LAB763
pQFNc_LAB763

sorghum

10596, 10505, 10596, 10505
393
611

LAB764
pUC19c_LAB764

sorghum

10487, 10516, 10487, 10508
394
751

LAB765
pUC19c_LAB765

sorghum

10597, 10890, 10597, 10869
395
613

LAB767
pMA-RQ_LAB767_GA

sorghum

396
614

LAB768
pQFNc_LAB768

sorghum

10698, 10838, 10698, 10955
397
615

LAB769
pQFNc_LAB769

sorghum

398
616

LAB770
pQFNc_LAB770

sorghum

11046, 11081, 11046, 11081
399
617

LAB771
pUC19c_LAB771

sorghum

10988, 10729, 11006, 11049
400
618

LAB772
pQFNc_LAB772

sorghum

10777, 10797, 10626, 10790
401
619

LAB773
TopoB_LAB773

sorghum

10481, 10602, 10482, 10607
402
620

LAB774
pQFNc_LAB774

sorghum

10629, 11079, 10629, 11079
403
621

LAB775
pUC19c_LAB775

sorghum

10511, 11060, 10511, 11060
404
622

LAB776
pQFNc_LAB776

sorghum

10787, 11070, 10773, 11068
405
623

LAB777
pQFNc_LAB777

sorghum

10624, 10840, 10624, 10840
406
624

LAB778
pQFNc_LAB778

sorghum

11023, 11076, 11023, 11076
407
625

LAB779
pQFNc_LAB779

sorghum

10579, 10896, 10697, 10914
408
626

LAB780
pQFNc_LAB780

sorghum

10996, 10650, 10996, 10675
409
627

LAB781
pQFNc_LAB781

sorghum

10993, 10676, 11031, 10679
410
628

LAB782
pUC19c_LAB782

sorghum

10702, 11074, 10759, 11069
411
629

LAB783
TopoB_LAB783

sorghum

412
630

LAB784
pUC19c_LAB784

sorghum

10628, 10464, 10628, 10464
413
631

LAB785
pMA_LAB785_GA

sorghum

414
632

LAB786
pQFNc_LAB786

sorghum

11024, 10723, 11024, 10622
415
633

LAB787
pUC19c_LAB787

sorghum

10718, 10850, 10747, 10920
416
634

LAB788
pQFNc_LAB788

sorghum

10768, 10886, 10730, 10894
417
635

LAB789
pUC19c_LAB789

sorghum

10704, 10851, 10786, 10839
418
752

LAB790
pQFNc_LAB790

sorghum

11012, 10561, 10969, 10532
419
753

LAB791
pQFNc_LAB791

sorghum

10625, 10798, 10663, 10958
420
638

LAB792
pUC19c_LAB792

sorghum

10655, 10954, 10637, 10819
421
639

LAB793
pQFNc_LAB793

sorghum

10774, 10909, 10716, 10801
422
640

LAB794
pUC19c_LAB794

sorghum

10651, 10926, 10649, 10855
423
641

LAB795
pUC19c_LAB795

sorghum

10497, 10567, 10490, 10569
424
642

LAB796
pQFNc_LAB796

sorghum

10769, 10900, 10633, 10830
425
643

LAB797
TopoB_LAB797

sorghum

10568, 10821, 10566, 10957
426
644

LAB798
pQFNc_LAB798

sorghum

10615, 10828, 10682, 11085
427
645

LAB799
pUC19c_LAB799

sorghum

10527, 10475, 10518, 10478
428
646

LAB800
pQFNc_LAB800
soybean
10767, 10868, 10669, 10864
429
647

LAB801
pUC19c_LAB801
soybean
10733, 10847, 10775, 10960
430
648

LAB802
TopoB_LAB802
soybean
10770, 10904, 10653, 10825
431
649

LAB803
pUC19c_LAB803
soybean
10732, 10947, 10590, 10905
432
650

LAB804
pQFNc_LAB804
soybean
10784, 10956, 10631, 10808
433
651

LAB805
pUC19c_LAB805
soybean
10627, 10933, 10599, 10860
434
652

LAB807
pQFNc_LAB807
soybean
10695, 10806, 10638, 10870
435
653

LAB809
pQFNc_LAB809
soybean
10772, 10791, 10580, 10887
436
754

LAB810
pQFNc_LAB810
soybean
10783, 10844, 10634, 10826
437
655

LAB811
pQFNc_LAB811
soybean
10715, 10950, 10576, 10941
438
656

LAB813
pQFNc_LAB813
sunflower
10617, 10852, 10778, 10903
439
755

LAB814
TopoB_LAB814
sunflower
11035, 10545, 11029, 10545
440
756

LAB815
pQFNc_LAB815
sunflower
10985, 10755, 11091, 11077
441
659

LAB816
pQFNc_LAB816
sunflower
10582, 10856, 10717, 10857
442
757

LAB817
pQFNc_LAB817
sunflower
10710, 10810, 10710, 10835
443
661

LAB820
pUC19c_LAB820
sunflower
10499, 10504, 10499, 10555
444
758

LAB821
pQFNc_LAB821
sunflower
10761, 10911, 10761, 10911
445
759

LAB823
pUC19c_LAB823
sunflower
10989, 10471, 11019, 10470
446
760

LAB824
pQFNc_LAB824
tomato
11048, 11087, 11075, 10923
447
665

LAB825
pQFNc_LAB825
tomato
10696, 10913, 10751, 10820
448
761

LAB827
pUC19_LAB827
tomato
10601, 10865, 10601, 10865
449
667

LAB829
pUC19c_LAB829
tomato
11093, 10744, 10975, 10776
450
668

LAB830
pQFNc_LAB830
tomato
10662, 10802, 10656, 10898
451
669

LAB831
pQFNc_LAB831
tomato
10588, 10803, 10588, 11086
452
670

LAB832
pQFNc_LAB832
tomato
10689, 10799, 10689, 10799
453
762

LAB833
pQFNc_LAB833
tomato
10731, 10792, 10737, 10871
454
672

LAB834
pUC19c_LAB834
tomato
10657, 10877, 10636, 10861
455
673

LAB835
pUC19c_LAB835
tomato
10752, 10879, 10754, 10878
456
674

LAB836
pUC19c_LAB836
tomato
10785, 10928, 10671, 10936
457
675

LAB837
pQFNc_LAB837
tomato
10708, 10794, 10589, 10822
458
763

LAB839
pQFNc_LAB839
tomato
11028, 10664, 10966, 10763
459
677

LAB840
pQFNc_LAB840
tomato
10762, 11054, 10711, 10918
460
678

LAB841
pQFNc_LAB841
tomato
10999, 10562, 11014, 10510
461
764

LAB842
pQFNc_LAB842
tomato
10575, 11088, 10743, 11089
462
680

LAB843
TopoB_LAB843
tomato
10764, 10945, 10764, 10945
463
681

LAB844
pQFNc_LAB844
wheat
10685, 10811, 10685, 10811
464
765

LAB845
pQFNc_LAB845
wheat
10604, 11082, 10604, 11082
465
766

LAB847
pQFNc_LAB847
wheat
11022, 11078, 11022, 11078
466
767

LAB848
pQFNc_LAB848
wheat
10728, 10853, 10728, 10853
467
768

LAB849
pQFNc_LAB849
wheat
10605, 10461, 10660, 10468
468
769

LAB850
pUC19c_LAB850
wheat
10507, 11063, 10507, 11063
469
770

Table 153: Provided are the sequence identifiers of the cloned genes, the primers used for cloning, genes' names, vectors used for cloning, and the plant species from which the genes were cloned.

Example 21
Transforming Agrobacterium Tumefaciens Cells with Binary Vectors Harboring the Polynucleotides of the Invention

Each of the binary vectors described in Example 20 above was used to transform Agrobacterium cells. Two additional binary constructs, having only the At6669 or the 35S promoter or no additional promoter were used as negative controls.

The binary vectors were introduced to Agrobacterium tumefaciens GV301, or LB4404 competent cells (about 10⁹cells/mL) by electroporation. The electroporation was performed using a MicroPulser electroporator (Biorad), 0.2 cm cuvettes (Biorad) and EC-2 electroporation program (Biorad). The treated cells were cultured in LB liquid medium at 28° C. for 3 hours, then plated over LB agar supplemented with gentamycin (50 mg/L; for Agrobacterium strains GV301) or streptomycin (300 mg/L; for Agrobacterium strain LB4404) and kanamycin (50 mg/L) at 28° C. for 48 hours. Abrobacterium colonies, which were developed on the selective media, were further analyzed by PCR using the primers designed to span the inserted sequence in the pPI plasmid. The resulting PCR products were isolated and sequenced to verify that the correct polynucleotide sequences of the invention are properly introduced to the Agrobacterium cells.

Example 22
Transformation of Arabidopsis Thaliana Plants with the Polynucleotides of the Invention

Arabidopsis thaliana Columbia plants (T₀plants) were transformed using the Floral Dip procedure described by Clough and Bent, 1998 (Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16:735-43) and by Desfeux et al., 2000 (Female Reproductive Tissues Are the Primary Target of Agrobacterium-Mediated Transformation by the Arabidopsis Floral-Dip Method. Plant Physiol, July 2000, Vol. 123, pp. 895-904), with minor modifications. Briefly, T₀Plants were sown in 250 ml pots filled with wet peat-based growth mix. The pots were covered with aluminum foil and a plastic dome, kept at 4° C. for 3-4 days, then uncovered and incubated in a growth chamber at 18-24° C. under 16/8 hour light/dark cycles. The T0 plants were ready for transformation six days before anthesis.

Single colonies of Agrobacterium carrying the binary constructs were generated as described in Example 21 above. Colonies were cultured in LB medium supplemented with kanamycin (50 mg/L) and gentamycin (50 mg/L). The cultures were incubated at 28° C. for 48 hours under vigorous shaking and then centrifuged at 4000 rpm for 5 minutes. The pellets comprising the Agrobacterium cells were re-suspended in a transformation medium containing half-strength (2.15 g/L) Murashige-Skoog (Duchefa); 0.044 μM benzylamino purine (Sigma); 112 μg/L B5 Gambourg vitamins (Sigma); 5% sucrose; and 0.2 ml/L Silwet L-77 (OSI Specialists, CT) in double-distilled water, at pH of 5.7.

Transformation of T₀plants was performed by inverting each plant into an Agrobacterium suspension, such that the above ground plant tissue was submerged for 3-5 seconds. Each inoculated T₀plant was immediately placed in a plastic tray, then covered with clear plastic dome to maintain humidity and was kept in the dark at room temperature for 18 hours, to facilitate infection and transformation. Transformed (transgenic) plants were then uncovered and transferred to a greenhouse for recovery and maturation. The transgenic T₀plants were grown in the greenhouse for 3-5 weeks until siliques were brown and dry. Seeds were harvested from plants and kept at room temperature until sowing.

For generating T₁and T₂transgenic plants harboring the genes, seeds collected from transgenic T₀plants were surface-sterilized by soaking in 70% ethanol for 1 minute, followed by soaking in 5% sodium hypochloride and 0.05% triton for 5 minutes. The surface-sterilized seeds are thoroughly washed in sterile distilled water then placed on culture plates containing half-strength Murashige-Skoog (Duchefa); 2% sucrose; 0.8% plant agar; 50 mM kanamycin; and 200 mM carbenicylin (Duchefa). The culture plates were incubated at 4° C. for 48 hours then transferred to a growth room at 25° C. for an additional week of incubation. Vital T₁Arabidopsis plants were transferred to a fresh culture plates for another week of incubation. Following incubation the T₁plants were removed from culture plates and planted in growth mix contained in 250 ml pots. The transgenic plants were allowed to grow in a greenhouse to maturity. Seeds harvested from T₁plants were cultured and grown to maturity as T₂plants under the same conditions as used for culturing and growing the T₁plants.

Example 23
Evaluation of Transgenic Arabidopsis Abst, Yield and Plant Growth Rate Under Abiotic Stress as Well as Under Standard Growth Conditions in Greenhouse Assay (GH-SM Assays)

Assay 1—ABST Measured Until Seed Yield (Seed Maturation Assay)

Seed yield, plant biomass and plant growth rate under drought conditions and standard growth conditions in greenhouse experiments—This assay follows seed yield production, the biomass formation and the rosette area growth of plants grown in the greenhouse under drought conditions and under standard growth conditions. Transgenic Arabidopsis seeds were sown in phytogel media supplemented with ½ MS medium and a selection agent (Kanamycin). The T₂transgenic seedlings were then transplanted to 1.7 trays filled with peat and perlite in a 1:2 ratio and tuff at the bottom of the tray and a net below the trays (in order to facilitate water drainage). Half of the plants were irrigated with tap water (standard growth conditions) when tray weight reached 50% of its field capacity. The other half of the plants were irrigated with tap water when tray weight reached 20% of its field capacity in order to induce drought stress. All plants were grown in the greenhouse until seeds maturation. Seeds were harvested, extracted and weighted. The remaining plant biomass (the above ground tissue) was also harvested, and weighted immediately or following drying in oven at 50° C. for 24 hours.

Each construct was validated at its T₂generation (under the control of the At6669 promoter, SEQ ID NO:10446). Transgenic plants transformed with a construct conformed by an empty vector carrying the At6669 (SEQ ID NO:10446) promoter and the selectable marker was used as control.

The plants were analyzed for their overall size, growth rate, flowering, seed yield, 1,000-seed weight, dry matter and harvest index (HI—seed yield/dry matter). Transgenic plants performance was compared to control plants grown in parallel under the same conditions. Mock-transgenic plants with no gene at all, under the same promoter were used as control.

The experiment was planned in nested randomized plot distribution. For each gene of the invention three to five independent transformation events were analyzed from each construct.

Digital imaging—A laboratory image acquisition system, which consists of a digital reflex camera (Canon EOS 300D) attached with a 55 mm focal length lens (Canon EF-S series), mounted on a reproduction device (Kaiser RS), which includes 4 light units (4×150 Watts light bulb) is used for capturing images of plant samples.

The image capturing process was repeated every 2 days starting from day 1 after transplanting till day 15. Same camera, placed in a custom made iron mount, was used for capturing images of larger plants sawn in white tubs in an environmental controlled greenhouse. The tubs were square shape include 1.7 liter trays. During the capture process, the tubs were placed beneath the iron mount, while avoiding direct sun light and casting of shadows.

An image analysis system was used, which consists of a personal desktop computer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ 1.39 [Java based image processing program which was developed at the U.S. National Institutes of Health and freely available on the internet at Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/]. Images were captured in resolution of 10 Mega Pixels (3888×2592 pixels) and stored in a low compression JPEG (Joint Photographic Experts Group standard) format. Next, analyzed data was saved to text files and processed using the JMP statistical analysis software (SAS institute).

Leaf analysis—Using the digital analysis leaves data was calculated, including leaf number, rosette area, rosette diameter, leaf blade area, Petiole Relative Area and leaf petiole length.

Vegetative growth rate: the relative growth rate (RGR) of leaf number [formula XXI (described above)], rosette area (Formula XXII, above), plot coverage (Formula XXIV, above) and harvest index (Formula IV) was calculated with the indicated formulas.

Seeds average weight—At the end of the experiment all seeds were collected. The seeds were scattered on a glass tray and a picture was taken. Using the digital analysis, the number of seeds in each sample was calculated.

Dry weight and seed yield—On about day 80 from sowing, the plants were harvested and left to dry at 30° C. in a drying chamber. The biomass and seed weight of each plot were measured and divided by the number of plants in each plot. Dry weight=total weight of the vegetative portion above ground (excluding roots) after drying at 30° C. in a drying chamber; Seed yield per plant=total seed weight per plant (gr.). 1000 seed weight (the weight of 1000 seeds) (gr.).

The harvest index (HI) was calculated using Formula IV as described above.

Statistical analyses—To identify genes conferring significantly improved tolerance to abiotic stresses, the results obtained from the transgenic plants were compared to those obtained from control plants. To identify outperforming genes and constructs, results from the independent transformation events tested were analyzed separately. Data was analyzed using Student's t-test and results were considered significant if the p value was less than 0.1. The JMP statistics software package was used (Version 5.2.1, SAS Institute Inc., Cary, N.C., USA).

Tables 154-163 summarize the observed phenotypes of transgenic plants exogenously expressing the gene constructs using the greenhouse seed maturation (GH-SM) assays standard growth conditions (Tables 154-158) conditions or under drought conditions (Tables 159-163). Transgenic plants expressing these genes exhibit higher biomass (Tables 154, 155, 157, 159, 160, 162), yield (Tables 157, 158, 162, 163), vigor and growth rate (Table 154, 156, 159 and 161), and flowering time (Tables 154 and 159) as compared to control plants grown under identical growth conditions. In addition, the increased of plant performance under drought stress, as compared to control plants, indicated increased tolerance to an abiotic stress condition such as drought. The evaluation of each gene was performed by testing the performance of different number of events. Event with p-value <0.1 was considered statistically significant. It should be noted that a negative increment (in percentages) when found in flowering or inflorescence emergence indicates drought avoidance of the plant.

TABLE 154

Genes showing improved plant performance at normal

growth conditions under regulation of At6669 promoter

Inflorescence

Dry Weight [mg]
Flowering (days)
Emergence (days)

Gene

P-
%

P-
%

P-
%

Name
Event #
Ave.
Val.
Incr.
Ave.
Val.
Incr.
Ave.
Val.
Incr.

LAB759
74848.1
—
—
—
—
—
—
17.2
0.09
−5

LAB759
74850.1
—
—
—
21.5
0.09
−3
—
—
—

LAB748
74976.2
—
—
—
—
—
—
17.3
0.20
−4

LAB719
74804.1
—
—
—
21.5
0.09
−3
—
—
—

LAB719
74805.2
—
—
—
21.7
0.28
−2
17.0
L
−6

LAB719
74807.2
—
—
—
21.5
0.09
−3
17.7
0.26
−2

LAB706
74800.2
—
—
—
21.7
0.28
−2
17.3
0.20
−4

LAB706
74803.4
—
—
—
21.7
0.28
−2
17.7
0.26
−2

LAB697
74949.1
—
—
—
21.5
0.09
−3
—
—
—

LAB697
74949.3
—
—
—
21.5
0.09
−3
—
—
—

LAB695
74796.1
—
—
—
21.5
0.09
−3
—
—
—

LAB693
74787.3
—
—
—
21.7
0.28
−2
—
—
—

LAB689
74756.3
—
—
—
21.5
0.09
−3
17.6
0.27
−3

LAB689
74760.1
—
—
—
21.0
0.20
−5
16.2
L
−10

LAB683
74874.1
—
—
—
21.7
0.28
−2
—
—
—

LAB675
74750.3
—
—
—
21.7
0.28
−2
—
—
—

LAB659
74835.1
—
—
—
21.2
0.14
−4
16.9
0.20
−7

LAB659
74836.1
—
—
—
21.7
0.28
−2
—
—
—

LAB659
74838.1
—
—
—
—
—
—
17.5
0.12
−3

LAB652
74859.2
—
—
—
21.5
0.09
−3
16.2
L
−10

LAB652
74861.1
—
—
—
21.5
0.09
−3
16.7
0.04
−8

LAB652
74863.1
—
—
—
21.5
0.09
−3
—
—
—

LAB626
74771.1
—
—
—
21.7
0.28
−2
—
—
—

CONT.
—
—
—
—
22.1
—
—
18.1
—
—

LAB759
74846.1
—
—
—
23.5
0.26
−1
—
—
—

LAB759
74850.1
—
—
—
23.5
0.26
−1
—
—
—

LAB748
74972.1
—
—
—
23.5
0.26
−1
—
—
—

LAB748
74974.1
1250.9
0.15
8
—
—
—
—
—
—

LAB748
74976.2
1365.2
0.05
18
—
—
—
—
—
—

LAB748
74977.3
—
—
—
23.5
0.26
−1
—
—
—

LAB732
74966.2
—
—
—
23.2
0.26
−2
—
—
—

LAB719
74804.1
—
—
—
22.7
0.03
−4
—
—
—

LAB719
74805.1
1212.5
0.20
5
—
—
—
—
—
—

LAB719
74805.2
—
—
—
22.9
L
−3
—
—
—

LAB706
74800.2
—
—
—
23.1
0.05
−3
—
—
—

LAB695
74793.1
—
—
—
23.2
0.26
−2
—
—
—

LAB694
74762.1
—
—
—
23.2
0.27
−2
—
—
—

LAB694
74764.3
1444.8
0.13
25
—
—
—
—
—
—

LAB693
74786.3
—
—
—
23.5
0.26
−1
—
—
—

LAB693
74787.1
—
—
—
23.2
0.27
−2
—
—
—

LAB693
74787.3
—
—
—
23.2
0.26
−2
—
—
—

LAB689
74756.2
—
—
—
—
—
—
16.4
L
−11

LAB689
74759.2
—
—
—
22.8
0.07
−4
16.3
L
−11

LAB689
74760.1
—
—
—
21.7
0.24
−9
16.0
L
−13

LAB683
74871.3
1492.8
0.17
29
—
—
—
—
—
—

LAB680
74775.1
1307.1
0.30
13
—
—
—
16.2
L
−12

LAB668
74864.2
—
—
—
23.5
0.26
−1
—
—
—

LAB668
74865.1
—
—
—
22.5
L
−5
16.2
L
−12

LAB668
74867.3
1255.0
0.17
9
23.3
0.22
−2
—
—
—

LAB666
74840.2
—
—
—
—
—
—
15.9
L
−14

LAB659
74839.1
1259.1
0.07
9
—
—
—
—
—
—

LAB626
74771.1
1483.6
0.08
29
—
—
—
16.0
L
−13

LAB626
74771.3
—
—
—
23.5
0.26
−1
—
—
—

CONT.
—
1153.9
—
—
23.7
—
—
18.4
—
—

Table 154. “CONT.” - Control; “Ave.” - Average; “% Incr.” = % increment; “p-val.” - p-value, L- p < 0.01.

TABLE 155

Genes showing improved plant performance at normal

growth conditions under regulation of At6669 promoter

Leaf Blade Area [cm²]
Leaf Number
Plot Coverage [cm²]

Gene

P-
%

P-
%

P-
%

Name
Event #
Ave.
Val.
Incr.
Ave.
Val.
Incr.
Ave.
Val.
Incr.

LAB748
74972.1
—
—
—
11.5
0.07
5
88.0
0.24
7

LAB748
74976.2
1.49
0.06
10
—
—
—
87.7
0.3
6

LAB732
74969.1
1.47
0.11
9
—
—
—
91.5
0.05
11

LAB697
74948.1
1.42
0.3
5
—
—
—
—
—
—

LAB689
74760.1
1.49
0.14
10
—
—
—
—
—
—

LAB675
74750.3
—
—
—
—
—
—
88.6
0.17
7

LAB659
74835.1
1.44
0.28
6
—
—
—
—
—
—

LAB659
74838.1
1.55
0.01
15
—
—
—
93.0
0.19
13

LAB652
74861.1
1.46
0.11
8
—
—
—
91.6
0.04
11

LAB652
74863.1
1.69
0.06
25
—
—
—
98.5
L
19

CONT.
—
1.35
—
—
11.0
—
—
82.6
—
—

LAB759
74846.1
1.59
0.2
5
10.9
0.08
4
91.9
0.05
12

LAB759
74850.1
—
—
—
11.2
L
7
—
—
—

LAB748
74972.1
—
—
—
10.9
0.13
4
—
—
—

LAB748
74977.2
1.59
0.22
5
—
—
—
88.6
0.16
8

LAB732
74966.1
—
—
—
11.1
0.16
6
—
—
—

LAB732
74966.2
—
—
—
10.9
0.08
4
—
—
—

LAB732
74967.1
—
—
—
—
—
—
87.0
0.28
6

LAB732
74971.1
—
—
—
11.1
0.02
6
—
—
—

LAB719
74804.1
—
—
—
—
—
—
90.6
0.08
11

LAB697
74950.2
—
—
—
11.1
0.03
6
88.1
0.18
8

LAB697
74951.1
1.66
0.03
10
—
—
—
89.1
0.22
9

LAB695
74792.3
—
—
—
10.8
0.28
3
—
—
—

LAB695
74796.1
—
—
—
10.9
0.05
4
—
—
—

LAB694
74767.2
1.69
0.02
12
—
—
—
92.9
0.04
13

LAB693
74787.3
—
—
—
10.8
0.29
3
—
—
—

LAB689
74756.2
—
—
—
—
—
—
90.4
0.09
10

LAB689
74759.2
1.72
0.29
14
—
—
—
92.4
0.15
13

LAB689
74760.1
1.68
0.07
12
11.4
L
9
95.1
0.02
16

LAB683
74874.1
—
—
—
10.9
0.08
4
—
—
—

LAB680
74775.1
1.83
0.09
21
11.0
0.07
5
—
—
—

LAB675
74750.3
1.61
0.28
7
—
—
—
91.4
0.08
12

LAB668
74864.2
1.58
0.25
5
—
—
—
—
—
—

LAB668
74865.1
1.70
0.19
13
—
—
—
93.1
0.27
14

LAB668
74867.3
—
—
—
11.4
0.03
9
—
—
—

LAB666
74840.2
1.79
0.08
19
10.8
0.29
3
99.2
0.12
21

LAB652
74860.2
—
—
—
10.8
0.2
3
—
—
—

LAB626
74771.1
1.61
0.24
7
—
—
—
—
—
—

CONT.
—
1.51
—
—
10.5
—
—
82.0
—
—

Table 155. “CONT.” - Control; “Ave.” - Average; “% Incr.” = % increment; “p-val.” - p-value, L- p < 0.01.

TABLE 156

Genes showing improved plant performance at normal

growth conditions under regulation of At6669 promoter

RGR Of Leaf
RGR Of Plot
RGR Of Rosette

Number (number
Coverage
Diameter

per day)
(cm²per day)
(cm per day)

Gene

P-
%

P-
%

P-
%

Name
Event #
Ave.
Val.
Incr.
Ave.
Val.
Incr.
Ave.
Val.
Incr.

LAB748
74972.1
0.84
0.29
15
—
—
—
—
—
—

LAB689
74760.1
—
—
—
13.2
0.25
15
0.56
0.27
11

LAB659
74838.1
—
—
—
—
—
—
0.57
0.24
11

LAB652
74863.1
—
—
—
13.9
0.1
21
0.57
0.19
12

CONT.
—
0.73
—
—
11.5
—
—
0.51
—
—

LAB759
74850.1
0.66
0.27
18
—
—
—
—
—
—

LAB732
74966.1
0.68
0.12
22
—
—
—
—
—
—

LAB732
74966.2
—
—
—
—
—
—
0.56
0.28
12

LAB732
74971.1
0.68
0.14
21
—
—
—
—
—
—

LAB719
74805.1
0.64
0.27
14
—
—
—
—
—
—

LAB719
74807.2
0.68
0.15
21
—
—
—
—
—
—

LAB697
74951.1
—
—
—
—
—
—
0.57
0.22
13

LAB695
74796.1
0.65
0.24
16
—
—
—
—
—
—

LAB689
74756.2
0.64
0.27
15
—
—
—
—
—
—

LAB689
74759.2
—
—
—
13.0
0.29
14
—
—
—

LAB689
74760.1
0.70
0.1
24
13.5
0.18
18
—
—
—

LAB683
74874.1
0.68
0.12
22
—
—
—
—
—
—

LAB680
74775.1
—
—
—
13.3
0.26
16
0.57
0.22
13

LAB668
74864.2
0.66
0.28
18
—
—
—
—
—
—

LAB668
74865.1
—
—
—
13.2
0.25
15
—
—
—

LAB668
74867.3
0.68
0.14
21
—
—
—
—
—
—

LAB666
74840.2
—
—
—
14.1
0.09
23
0.59
0.08
19

LAB666
74841.2
0.74
0.04
32
—
—
—
—
—
—

LAB666
74844.3
0.69
0.14
23
—
—
—
—
—
—

LAB659
74836.2
0.66
0.22
18
—
—
—
—
—
—

LAB652
74859.1
0.66
0.25
18
—
—
—
—
—
—

CONT.
—
0.56
—
—
11.5
—
—
0.50
—
—

Table 156. “CONT.” - Control; “Ave.” - Average; “% Incr.” = % increment; “p-val.” - p-value, L- p < 0.01.

TABLE 157

Genes showing improved plant performance at normal

growth conditions under regulation of At6669 promoter

Harvest Index
Rosette Area [cm²]
Rosette Diameter [cm]

Gene

P-
%

P-
%

P-
%

Name
Event #
Ave.
Val.
Incr.
Ave.
Val.
Incr.
Ave.
Val.
Incr.

LAB748
74972.1
—
—
—
11.0
0.24
7
—
—
—

LAB748
74976.2
—
—
—
11.0
0.3
6
5.5
0.21
4

LAB732
74969.1
—
—
—
11.4
0.05
11
5.6
0.14
6

LAB697
74948.1
—
—
—
—
—
—
5.5
0.29
3

LAB675
74750.3
—
—
—
11.1
0.17
7
—
—
—

LAB659
74835.1
—
—
—
—
—
—
5.5
0.28
4

LAB659
74838.1
—
—
—
11.6
0.19
13
5.8
0.07
9

LAB652
74861.1
—
—
—
11.5
0.04
11
5.6
0.05
6

LAB652
74863.1
—
—
—
12.3
L
19
5.9
L
12

CONT.
—
—
—
—
10.3
—
—
5.3
—
—

LAB759
74846.1
—
—
—
11.5
0.05
12
5.6
0.05
6

LAB759
74850.1
0.50
0.14
12
—
—
—
—
—
—

LAB748
74972.1
0.50
0.03
13
—
—
—
—
—
—

LAB748
74977.2
0.53
L
18
11.1
0.16
8
—
—
—

LAB732
74966.2
0.52
0.02
17
—
—
—
5.5
0.27
4

LAB732
74967.1
—
—
—
10.9
0.28
6
—
—
—

LAB719
74804.1
0.53
0.07
19
11.3
0.08
11
5.5
0.12
4

LAB719
74807.2
0.50
0.27
13
—
—
—
—
—
—

LAB706
74803.4
0.48
0.2
7
—
—
—
—
—
—

LAB697
74949.1
0.48
0.25
7
—
—
—
—
—
—

LAB697
74950.2
0.50
0.03
13
11.0
0.18
8
5.5
0.28
3

LAB697
74951.1
—
—
—
11.1
0.22
9
5.7
0.03
8

LAB695
74792.3
0.48
0.26
8
—
—
—
—
—
—

LAB695
74793.1
0.49
0.06
11
—
—
—
—
—
—

LAB695
74796.1
0.51
0.07
14
—
—
—
—
—
—

LAB695
74797.4
0.48
0.16
7
—
—
—
—
—
—

LAB694
74767.1
0.53
0.07
18
—
—
—
—
—
—

LAB694
74767.2
0.48
0.29
7
11.6
0.04
13
5.5
0.11
4

LAB689
74756.2
—
—
—
11.3
0.09
10
5.6
0.09
5

LAB689
74756.3
0.49
0.07
11
—
—
—
—
—
—

LAB689
74759.2
—
—
—
11.6
0.15
13
5.7
0.06
7

LAB689
74760.1
—
—
—
11.9
0.02
16
5.7
0.03
7

LAB683
74872.3
0.53
L
18
—
—
—
—
—
—

LAB683
74873.1
0.53
L
20
—
—
—
—
—
—

LAB680
74774.1
0.49
0.08
10
—
—
—
—
—
—

LAB680
74775.1
—
—
—
12.8
0.12
25
5.9
0.1
10

LAB680
74779.1
0.49
0.2
10
—
—
—
—
—
—

LAB675
74750.3
—
—
—
11.4
0.08
12
5.5
0.13
4

LAB675
74751.4
0.54
0.1
21
—
—
—
—
—
—

LAB668
74864.2
—
—
—
11.2
0.13
9
—
—
—

LAB668
74865.1
0.49
0.23
9
11.6
0.27
14
5.7
0.1
7

LAB666
74840.2
—
—
—
12.4
0.12
21
5.9
0.04
11

LAB666
74840.3
0.51
0.09
15
—
—
—
—
—
—

LAB666
74844.3
0.50
0.18
13
—
—
—
—
—
—

LAB659
74836.1
0.49
0.21
10
—
—
—
—
—
—

LAB659
74838.1
—
—
—
—
—
—
5.5
0.28
4

LAB626
74770.2
0.49
0.15
10
—
—
—
—
—
—

LAB626
74771.2
0.53
0.11
20
—
—
—
—
—
—

CONT.
—
0.44
—
—
10.2
—
—
5.3
—
—

Table 157. “CONT.” - Control; “Ave.” - Average; “% Incr.” = % increment; “p-val.” - p-value, L- p < 0.01.

TABLE 158

Genes showing improved plant performance at normal

growth conditions under regulation of At6669 promoter

Gene

Seed Yield [mg]
1000 Seed Weight [mg]

Name
Event #
Ave.
P-Val.
% Incr.
Ave.
P-Val.
% Incr.

LAB748
74972.1
563.2
0.07
11
—
—
—

LAB748
74976.2
—
—
—
27.2
0.04
11

LAB732
74966.2
587.1
0.04
15
—
—
—

LAB719
74804.1
535.1
0.27
5
—
—
—

LAB719
74805.1
—
—
—
27.2
0.02
11

LAB719
74805.2
—
—
—
28.2
0.02
15

LAB719
74808.1
—
—
—
28.9
L
18

LAB706
74801.4
676.9
0.10
33
—
—
—

LAB695
74796.1
617.2
0.23
21
—
—
—

LAB694
74764.3
—
—
—
28.2
0.21
15

LAB689
74756.3
538.9
0.16
6
—
—
—

LAB683
74871.3
—
—
—
32.0
L
31

LAB683
74872.3
558.1
0.06
10
—
—
—

LAB683
74873.1
610.9
0.16
20
—
—
—

LAB680
74777.1
—
—
—
25.6
0.20
5

LAB680
74777.2
—
—
—
28.2
L
15

LAB675
74750.3
541.4
0.13
6
—
—
—

LAB675
74751.4
608.9
L
20
—
—
—

LAB668
74864.1
564.4
0.03
11
—
—
—

LAB668
74865.1
583.0
0.14
14
—
—
—

LAB668
74867.3
540.8
0.13
6
—
—
—

LAB668
74867.6
547.2
0.16
7
—
—
—

LAB666
74840.3
586.5
L
15
—
—
—

LAB659
74835.1
—
—
—
26.3
0.01
7

LAB659
74836.2
568.4
0.02
12
—
—
—

LAB652
74859.1
—
—
—
26.3
0.01
7

LAB652
74861.1
—
—
—
26.2
0.22
7

LAB652
74863.1
—
—
—
27.2
L
11

LAB626
74771.1
—
—
—
28.2
0.15
15

LAB626
74771.2
594.1
L
17
—
—
—

CONT.
—
509.3
—
—
24.5
—
—

Table 158. “CONT.” - Control; “Ave.” - Average; “% Incr.” = % increment; “p-val.” - p-value, L- p < 0.01.

TABLE 159

Genes showing improved plant performance at drought growth

conditions under regulation of At6669 promoter

Inflorescence

Dry Weight [mg]
Flowering (days)
Emergence (days)

Gene

%

P-
%

P-
%

Name
Event #
Ave
P-Val.
Incr.
Ave.
Val.
Incr.
Ave.
Val.
Incr.

LAB759
74848.1
—
—
—
—
—
—
17.7
0.09
−5

LAB759
74850.1
—
—
—
—
—
—
18.0
0.13
−4

LAB719
74804.1
—
—
—
—
—
—
17.7
0.10
−5

LAB719
74805.2
—
—
—
—
—
—
17.7
0.10
−5

LAB706
74800.2
—
—
—
—
—
—
17.7
0.04
−5

LAB697
74949.3
—
—
—
—
—
—
18.1
0.21
−3

LAB695
74793.1
—
—
—
—
—
—
18.1
0.17
−3

LAB695
74796.1
—
—
—
—
—
—
17.7
0.10
−5

LAB693
74787.1
—
—
—
—
—
—
18.0
0.13
−4

LAB693
74787.3
—
—
—
—
—
—
17.9
0.14
−4

LAB689
74756.2
—
—
—
—
—
—
18.0
0.13
−4

LAB689
74759.2
—
—
—
—
—
—
17.6
0.04
−6

LAB689
74760.1
—
—
—
20.8
L
−7
16.4
0.10
−12

LAB683
74872.3
—
—
—
—
—
—
18.1
0.17
−3

LAB683
74874.1
—
—
—
—
—
—
18.1
0.20
−3

LAB680
74775.1
—
—
—
21.2
0.09
−5
17.3
0.05
−7

LAB675
74751.3
—
—
—
21.5
0.03
−4
17.0
0.16
−9

LAB668
74864.1
—
—
—
—
—
—
18.1
0.17
−3

LAB668
74864.2
—
—
—
—
—
—
18.1
0.17
−3

LAB659
74835.1
—
—
—
21.7
0.11
−3
17.2
0.02
−8

LAB652
74861.1
—
—
—
—
—
—
18.1
0.17
−3

LAB652
74863.1
—
—
—
—
—
—
17.9
0.23
−4

CONT.
—
—
—
—
22.3
—
—
18.7
—
—

LAB759
74846.3
874.4
0.29
5
—
—
—
—
—
—

LAB759
74848.4
896.9
0.03
7
—
—
—
—
—
—

LAB719
74805.1
906.8
0.02
8
—
—
—
—
—
—

LAB719
74805.2
996.2
0.20
19
—
—
—
—
—
—

LAB719
74807.2
—
—
—
—
—
—
19.2
0.17
−3

LAB706
74801.2
995.9
0.01
19
23.7
0.04
−2
—
—
—

LAB706
74801.4
886.2
0.25
6
—
—
—
—
—
—

LAB706
74802.2
879.4
0.19
5
—
—
—
—
—
—

LAB697
74951.1
956.9
0.28
14
—
—
—
—
—
—

LAB695
74792.3
—
—
—
—
—
—
18.9
0.27
−5

LAB695
74797.4
881.2
0.17
5
—
—
—
—
—
—

LAB694
74762.1
899.4
0.24
8
23.5
L
−3
19.1
L
−4

LAB694
74767.2
—
—
—
23.4
0.01
−3
18.7
0.10
−5

LAB693
74786.3
891.2
0.15
7
—
—
—
—
—
—

LAB693
74786.4
891.9
0.05
7
—
—
—
—
—
—

LAB693
74787.3
—
—
—
23.5
0.10
−3
—
—
—

LAB689
74759.2
—
—
—
22.9
0.09
−5
—
—
—

LAB689
74760.1
—
—
—
23.2
0.10
−4
16.4
L
−17

LAB683
74871.3
1045.6
0.05
25
—
—
—
—
—
—

LAB680
74775.1
1039.8
0.06
24
23.7
0.04
−2
—
—
—

LAB680
74777.2
948.8
0.14
14
—
—
—
19.0
0.20
−4

LAB680
74779.1
883.8
0.21
6
—
—
—
—
—
—

LAB675
74750.2
1046.2
0.08
25
—
—
—
—
—
—

LAB675
74751.3
—
—
—
23.1
L
−5
18.6
0.02
−6

LAB675
74751.4
878.1
0.10
5
—
—
—
—
—
—

LAB668
74867.3
905.6
0.27
8
—
—
—
—
—
—

LAB666
74840.2
885.6
0.06
6
22.9
0.24
−5
16.3
L
−17

LAB666
74844.3
—
—
—
—
—
—
19.1
0.23
−3

LAB659
74835.1
1028.8
0.05
23
—
—
—
—
—
—

LAB659
74836.1
906.2
0.02
8
—
—
—
—
—
—

LAB659
74839.1
1023.3
L
22
—
—
—
—
—
—

LAB652
74859.1
1109.2
0.24
33
—
—
—
—
—
—

LAB652
74861.1
1050.5
0.01
26
—
—
—
18.8
0.30
−5

LAB652
74863.1
953.1
L
14
—
—
—
—
—
—

LAB626
74770.2
—
—
—
23.7
0.04
−2
—
—
—

LAB626
74771.1
1108.1
L
33
—
—
—
—
—
—

LAB626
74771.2
882.2
0.07
6
—
—
—
—
—
—

LAB626
74773.1
909.4
0.29
9
—
—
—
—
—
—

CONT.
—
835.9
—
—
24.2
—
—
19.8
—
—

Table 159. “CONT.” - Control; “Ave.” - Average; “% Incr.” = % increment; “p-val.” - p-value, L- p < 0.01.

TABLE 160

Genes showing improved plant performance at drought growth

conditions under regulation of At6669 promoter

Leaf Blade Area [cm²]
Leaf Number
Plot Coverage [cm²]

Gene

P-
%

p-
%

p-
%

Name
Event #
Ave.
Val.
Incr.
Ave.
Val.
Incr.
Ave.
Val.
Incr.

LAB759
74848.1
—
—
—
11.5
L
9
—
—
—

LAB732
74966.2
—
—
—
10.9
0.09
3
—
—
—

LAB732
74969.1
—
—
—
11.3
0.06
7
—
—
—

LAB719
74805.2
—
—
—
11.1
0.02
5
—
—
—

LAB706
74800.2
1.51
L
27
12.2
0.19
15
93.1
0.01
30

LAB706
74803.4
—
—
—
11.1
0.2
5
—
—
—

LAB695
74793.1
1.24
0.24
5
—
—
—
—
—
—

LAB695
74796.1
1.28
0.22
8
—
—
—
—
—
—

LAB693
74787.1
—
—
—
11.3
0.2
7
76.0
0.3
6

LAB693
74787.3
1.29
0.17
9
10.8
0.24
2
—
—
—

LAB689
74756.1
—
—
—
10.8
0.24
2
—
—
—

LAB689
74759.2
—
—
—
11.0
0.1
4
—
—
—

LAB683
74874.1
1.37
0.19
15
11.1
0.04
5
84.7
0.16
18

LAB675
74751.3
—
—
—
—
—
—
74.6
0.29
4

LAB668
74864.2
1.27
0.12
7
—
—
—
74.9
0.24
4

LAB659
74835.1
1.28
0.08
8
—
—
—
81.0
0.01
13

LAB652
74861.1
1.41
0.04
19
—
—
—
—
—
—

LAB652
74863.1
—
—
—
11.4
0.01
8
74.8
0.26
4

CONT.
—
1.19
—
—
10.6
—
—
71.9
—
—

LAB759
74848.4
—
—
—
—
—
—
80.9
0.04
16

LAB759
74850.1
—
—
—
—
—
—
76.2
0.17
9

LAB748
74972.1
—
—
—
—
—
—
75.6
0.28
8

LAB732
74966.1
—
—
—
—
—
—
77.6
0.17
11

LAB732
74967.1
—
—
—
11.1
0.17
6
—
—
—

LAB719
74804.1
—
—
—
11.1
0.09
6
—
—
—

LAB706
74800.2
1.38
0.2
7
—
—
—
76.0
0.29
9

LAB695
74797.4
1.39
0.21
7
—
—
—
—
—
—

LAB694
74762.1
—
—
—
10.9
0.19
4
—
—
—

LAB694
74767.2
1.46
0.07
13
—
—
—
81.7
0.25
17

LAB693
74786.3
1.42
0.09
10
—
—
—
—
—
—

LAB693
74787.1
—
—
—
11.2
0.06
7
—
—
—

LAB689
74756.2
1.43
0.21
11
11.1
0.11
6
86.3
0.02
23

LAB689
74756.3
1.48
0.1
14
—
—
—
80.7
0.06
15

LAB689
74759.2
1.43
0.12
11
—
—
—
77.2
0.14
10

LAB689
74760.1
1.39
0.3
8
—
—
—
75.0
0.3
7

LAB683
74874.1
—
—
—
11.2
0.06
7
77.5
0.2
11

LAB675
74751.3
—
—
—
11.4
0.03
9
—
—
—

LAB668
74864.2
—
—
—
10.9
0.27
4
—
—
—

LAB668
74865.1
1.54
L
19
—
—
—
83.5
0.02
19

LAB666
74840.2
1.52
0.01
18
—
—
—
84.8
0.01
21

LAB666
74840.3
—
—
—
11.2
0.2
7
—
—
—

LAB666
74841.2
—
—
—
11.1
0.17
6
79.3
0.07
13

CONT.
—
1.29
—
—
10.5
—
—
69.9
—
—

“CONT.”—Control;

“Ave.”—Average;

“% Incr.” = % increment;

“p-val.”—p-value,

L—p < 0.01.

TABLE 161

Genes showing improved plant performance at drought growth

conditions under regulation of At6669 promoter

RGR Of
RGR Of

Leaf Number
Plot Coverage
RGR Of Rosette

(number/day)
(cm²/day)
Diameter (cm/day)

Gene

P-
%

P-
%

P-
%

Name
Event #
Ave.
Val.
Incr.
Ave.
Val.
Incr.
Ave.
Val.
Incr.

LAB759
74848.1
—
—
—
11.7
0.13
17
0.505
0.14
11

LAB732
74969.1
—
—
—
—
—
—
0.508
0.13
11

LAB706
74800.2
—
—
—
12.9
L
29
0.518
0.06
14

LAB693
74787.1
0.8
0.15
21
—
—
—
—
—
—

LAB693
74787.3
—
—
—
—
—
—
0.496
0.21
9

LAB683
74874.1
—
—
—
11.7
0.11
17
—
—
—

LAB659
74835.1
—
—
—
11.3
0.18
14
—
—
—

LAB652
74861.1
—
—
—
11.7
0.14
17
—
—
—

CONT.
—
0.7
—
—
10.0
—
—
0.456
—
—

LAB759
74848.4
—
—
—
11.3
0.16
19
—
—
—

LAB694
74767.2
—
—
—
11.3
0.17
19
—
—
—

LAB689
74756.2
—
—
—
11.8
0.08
24
—
—
—

LAB689
74756.3
—
—
—
10.8
0.29
14
—
—
—

LAB675
74751.3
0.7
0.13
31
—
—
—
—
—
—

LAB668
74864.2
0.7
0.23
24
—
—
—
—
—
—

LAB668
74865.1
—
—
—
11.2
0.20
17
—
—
—

LAB666
74840.2
—
—
—
11.6
0.10
23
—
—
—

LAB666
74841.2
0.7
0.29
21
10.9
0.26
15
—
—
—

CONT.
—
0.6
—
—
9.5
—
—
—
—
—

“CONT.”—Control;

“Ave.”—Average;

“% Incr.” = % increment;

“p-val.”—p-value,

L—p < 0.01.

TABLE 162

Genes showing improved plant performance at drought growth

conditions under regulation of At6669 promoter

Rosette

Gene

Harvest Index
Rosette Area [cm2]
Diameter [cm]

Name
Event #
Ave.
P-Val.
% Incr.
Ave.
P-Val.
% Incr.
Ave.
P-Val.
% Incr.

LAB732
74969.1
—
—
—
—
—
—
5.35
0.25
8

LAB706
74800.2
—
—
—
11.6
0.01
30
5.69
L
15

LAB693
74787.1
—
—
—
9.5
0.3
6
5.05
0.3
2

LAB689
74759.2
—
—
—
—
—
—
5.10
0.21
3

LAB683
74874.1
—
—
—
10.6
0.16
18
5.32
0.03
7

LAB675
74751.3
—
—
—
9.3
0.29
4
—
—
—

LAB668
74864.2
—
—
—
9.4
0.24
4
5.16
0.25
4

LAB659
74835.1
—
—
—
10.1
0.01
13
5.24
0.13
6

LAB652
74863.1
—
—
—
9.3
0.26
4
—
—
—

CONT.
—
—
—
—
9.0
—
—
4.96
—
—

LAB759
74846.1
—
—
—
—
—
—
5.14
0.24
4

LAB759
74847.1
0.46
0.15
21
—
—
—
—
—
—

LAB759
74848.4
—
—
—
10.1
0.04
16
5.13
0.26
4

LAB759
74850.1
0.48
0.14
25
9.5
0.17
9
—
—
—

LAB748
74972.1
—
—
—
9.5
0.28
8
—
—
—

LAB748
74977.2
0.44
0.21
16
—
—
—
—
—
—

LAB748
74977.3
0.53
0.01
40
—
—
—
—
—
—

LAB732
74966.1
—
—
—
9.7
0.17
11
5.12
0.3
3

LAB732
74967.1
0.47
L
23
—
—
—
—
—
—

LAB706
74800.2
—
—
—
9.5
0.29
9
—
—
—

LAB697
74949.1
0.43
0.08
13
—
—
—
—
—
—

LAB697
74949.3
0.43
0.27
12
—
—
—
—
—
—

LAB695
74796.1
0.42
0.23
9
—
—
—
—
—
—

LAB695
74797.4
0.43
0.03
13
—
—
—
—
—
—

LAB694
74767.2
—
—
—
10.2
0.25
17
5.32
0.28
7

LAB693
74786.3
—
—
—
—
—
—
5.19
0.28
5

LAB689
74756.1
0.47
0.25
22
—
—
—
—
—
—

LAB689
74756.2
0.40
0.28
6
10.8
0.02
23
5.45
0.03
10

LAB689
74756.3
—
—
—
10.1
0.06
15
5.25
0.25
6

LAB689
74759.2
—
—
—
9.6
0.14
10
—
—
—

LAB689
74760.1
0.46
0.04
20
9.4
0.3
7
—
—
—

LAB683
74874.1
—
—
—
9.7
0.2
11
—
—
—

LAB680
74774.1
0.42
0.19
10
—
—
—
—
—
—

LAB675
74750.2
—
—
—
—
—
—
5.17
0.2
4

LAB675
74750.3
0.43
0.19
12
—
—
—
—
—
—

LAB675
74751.4
0.42
0.04
10
—
—
—
—
—
—

LAB675
74753.1
0.43
0.07
13
—
—
—
—
—
—

LAB668
74865.1
—
—
—
10.4
0.02
19
5.20
0.17
5

LAB666
74840.2
0.42
0.08
9
10.6
0.01
21
5.29
0.06
7

LAB666
74841.2
—
—
—
9.9
0.07
13
—
—
—

LAB626
74771.3
0.42
0.11
11
—
—
—
—
—
—

CONT.
—
0.38
—
—
8.7
—
—
4.95
—
—

“CONT.”—Control;

“Ave.”—Average;

“% Incr.” = % increment;

“p-val.”—p-value,

L—p < 0.01.

TABLE 163

Genes showing improved plant performance at drought growth

conditions under regulation of At6669 promoter

Seed Yield [mg]
1000 Seed Weight [mg]

Gene Name
Event #
Ave.
P-Val.
% Incr.
Ave.
P-Val.
% Incr.

LAB759
74847.1
363.3
0.06
14
—
—
—

LAB759
74850.1
397.4
0.16
24
—
—
—

LAB748
74977.3
414.7
0.05
30
—
—
—

LAB732
74967.1
371.5
L
16
—
—
—

LAB719
74805.2
—
—
—
27.9
0.15
17

LAB719
74807.2
377.4
0.04
18
—
—
—

LAB697
74949.1
367.0
L
15
—
—
—

LAB697
74949.3
362.1
0.26
13
—
—
—

LAB695
74795.1
—
—
—
24.8
0.15
4

LAB695
74796.1
345.7
0.11
8
—
—
—

LAB695
74797.4
380.6
0.06
19
—
—
—

LAB694
74762.1
—
—
—
26.3
L
10

LAB694
74764.3
—
—
—
26.8
0.21
13

LAB689
74760.1
377.7
0.08
18
—
—
—

LAB683
74871.3
—
—
—
29.4
0.14
23

LAB680
74775.1
—
—
—
28.5
0.04
19

LAB680
74777.2
—
—
—
26.5
L
11

LAB675
74750.3
379.1
L
19
—
—
—

LAB675
74751.3
—
—
—
26.5
0.26
11

LAB675
74751.4
370.2
L
16
—
—
—

LAB675
74753.1
360.2
0.06
13
—
—
—

LAB668
74864.1
376.4
0.14
18
—
—
—

LAB668
74865.1
378.2
0.18
18
—
—
—

LAB668
74867.3
339.9
0.21
6
—
—
—

LAB666
74840.2
370.0
L
16
—
—
—

LAB659
74835.1
—
—
—
26.9
L
13

LAB659
74836.1
—
—
—
24.5
0.21
3

LAB659
74839.1
—
—
—
28.1
0.11
18

LAB652
74859.1
—
—
—
27.2
L
14

LAB652
74861.1
—
—
—
28.1
L
18

LAB626
74771.1
—
—
—
29.0
0.03
22

CONT.
—
319.3
—
—
23.8
—
—

“CONT.”—Control;

“Ave.”—Average;

“% Incr.” = % increment;

“p-val.”—p-value,

L—p < 0.01.

Example 24
Evaluation of Transgenic Arabidopsis Abst, Biomass and Plant Growth Rate Under Abiotic Stress as Well as Under Standard Conditions in Greenhouse Assay (GH-SB Assays)

Assay 2—Abst Measured Until Bolting Stage.

Plant biomass and plant growth rate under drought conditions and standard growth conditions in greenhouse experiments—This assay follows the plant biomass formation and the rosette area growth of plants grown in the greenhouse under drought conditions and standard growth conditions. Transgenic Arabidopsis seeds were sown in phytogel media supplemented with ½ MS medium and a selection agent (Kanamycin). The T₂transgenic seedlings were then transplanted to 1.7 trays filled with peat and perlite in a 1:2 ratio and tuff at the bottom of the tray and a net below the trays (in order to facilitate water drainage). Half of the plants were irrigated with tap water (standard growth conditions) when tray weight reached 50% of its field capacity. The other half of the plants were irrigated with tap water when tray weight reached 20% of its field capacity in order to induce drought stress (drought conditions). All plants are grown in the greenhouse until bolting stage. At harvest, plant biomass (the above ground tissue) was weighted directly after harvesting the rosette (plant fresh weight [FW]). Thereafter, plants were dried in an oven at 50° C. for 48 hours and weighted (plant dry weight [DW]).

Each construct was validated at its T₂generation (under the control of the At6669 promoter, SEQ ID NO:10446). Transgenic plants transformed with a construct conformed by an empty vector carrying the At6669 promoter (SEQ ID NO:10446) and the selectable marker was used as control.

The plants were analyzed for their overall size, growth rate, fresh weight and dry matter. Transgenic plants performance was compared to control plants grown in parallel under the same conditions. Mock-transgenic plants with no gene at all, under the same promoter were used as control.

The experiment was planned in nested randomized plot distribution. For each gene of the invention three to five independent transformation events were analyzed from each construct.

Digital imaging—A laboratory image acquisition system, which consists of a digital reflex camera (Canon EOS 300D) attached with a 55 mm focal length lens (Canon EF-S series), mounted on a reproduction device (Kaiser RS), which included 4 light units (4×150 Watts light bulb) is used for capturing images of plant samples.

The image capturing process was repeated every 2 days starting from day 1 after transplanting till day 16. Same camera, placed in a custom made iron mount, was used for capturing images of larger plants sawn in white tubs in an environmental controlled greenhouse. The tubs were square shape include 1.7 liter trays. During the capture process, the tubs were placed beneath the iron mount, while avoiding direct sun light and casting of shadows.

An image analysis system was used, which consists of a personal desktop computer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ 1.39 (Java based image processing program which was developed at the U.S National Institutes of Health and freely available on the internet at Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/). Images were captured in resolution of 10 Mega Pixels (3888×2592 pixels) and stored in a low compression JPEG (Joint Photographic Experts Group standard) format. Next, analyzed data was saved to text files and processed using the JMP statistical analysis software (SAS institute).

Leaf analysis—Using the digital analysis leaves data was calculated, including leaf number, rosette area, rosette diameter, leaf blade area, Petiole Relative Area and leaf petiole length.

Plant Fresh and Dry weight—On about day 80 from sowing, the plants were harvested and directly weight for the determination of the plant fresh weight (FW) and left to dry at 50° C. in a drying chamber for about 48 hours before weighting to determine plant dry weight (DW).

Experimental Results:

The genes were cloned under the regulation of a constitutive (At6669; SEQ ID NO: 10446). The evaluation of each gene was performed by testing the performance of different number of events. Event with p-value <0.1 was considered statistically significant.

The genes listed in Tables 164-166 improved plant ABST when grown under drought conditions. These genes produced larger plants with a larger photosynthetic area and increased biomass and growth rate [dry weight, fresh weight, leaf number, rosette diameter, rosette area, plot coverage, growth rate (RGR) of leaf number, growth rate (RGR) of plot coverage, growth rate (RGR) of rosette diameter] when grown under drought conditions.

The genes listed in Tables 167-169 improved plant performance when grown under standard (normal) growth conditions. These genes produced larger plants with a larger photosynthetic area and increased biomass and growth rate [dry weight, fresh weight, leaf number, rosette diameter, rosette area, plot coverage, growth rate (RGR) of leaf number, growth rate (RGR) of plot coverage, growth rate (RGR) of rosette diameter] when grown under normal conditions.

TABLE 164

Genes showing improved plant performance at drought growth

conditions under regulation of A6669 promoter

Dry Weight [mg]
Fresh Weight [mg]
Leaf Number

P-
%

P-
%

P-
%

Gene Name
Event #
Ave.
Val.
Incr.
Ave.
Val.
Incr.
Ave.
Val.
Incr.

LAB824
75505.8
106.9
0.19
16
—
—
—
—
—
—

LAB795
76491.3
116.9
0.08
26
1381.2
0.14
26
10.2
0.05
9

LAB795
76494.5
102.1
0.28
10
—
—
—
—
—
—

LAB795
76494.6
—
—
—
—
—
—
9.9
0.14
6

LAB792
75876.1
—
—
—
—
—
—
9.8
0.28
5

LAB792
75876.3
—
—
—
—
—
—
10.1
0.08
8

LAB790
75713.2
114.4
0.03
24
1387.5
0.03
27
9.9
0.27
6

LAB789
77081.1
—
—
—
1237.5
0.22
13
—
—
—

LAB784
76466.3
108.6
0.09
17
1225.9
0.26
12
—
—
—

LAB784
76467.4
121.2
0.20
31
—
—
—
10.6
0.02
13

LAB784
76468.4
102.5
0.26
11
—
—
—
—
—
—

LAB784
76470.2
—
—
—
—
—
—
10.2
0.25
9

LAB769
75348.4
113.7
0.06
23
—
—
—
—
—
—

LAB750
75447.2
116.2
0.22
26
1418.8
0.02
30
9.8
0.28
4

LAB730
75180.2
113.5
0.04
23
1364.3
0.07
25
10.5
0.04
12

LAB729
77303.10
—
—
—
—
—
—
9.8
0.28
5

LAB729
77303.7
113.8
0.06
23
1362.5
0.27
25
—
—
—

LAB713
75092.1
—
—
—
1243.8
0.21
14
—
—
—

LAB670
75303.1
—
—
—
1429.5
0.02
31
—
—
—

LAB656
77320.6
109.4
0.09
18
1393.8
0.22
27
10.6
0.22
13

LAB649
77041.3
—
—
—
1298.2
0.17
19
—
—
—

LAB649
77044.2
107.0
0.16
16
—
—
—
—
—
—

LAB649
77045.3
118.8
0.02
28
1293.8
0.11
18
—
—
—

LAB637
75105.5
103.7
0.22
12
—
—
—
—
—
—

LAB637
75105.7
—
—
—
1305.4
0.09
19
—
—
—

LAB637
75105.8
—
—
—
—
—
—
10.4
0.17
12

LAB636
75100.3
122.5
0.03
32
1556.2
0.09
42
10.3
0.11
10

LAB636
75104.8
105.0
0.27
14
1425.0
0.15
30
10.2
0.05
9

CONT.
—
92.5
—
—
1093.9
—
—
9.4
—
—

LAB839
75624.1
57.5
0.13
14
531.2
0.02
30
—
—
—

LAB809
75653.2
60.0
0.01
19
519.6
L
28
—
—
—

LAB805
76117.2
—
—
—
462.5
0.20
14
—
—
—

LAB798
75615.4
—
—
—
450.0
0.11
10
—
—
—

LAB798
75616.2
54.7
0.16
8
473.2
0.01
16
—
—
—

LAB792
75876.1
58.1
0.29
15
437.5
0.15
7
—
—
—

LAB792
75876.2
—
—
—
—
—
—
11.6
L
7

LAB790
75711.1
—
—
—
—
—
—
11.8
L
8

LAB790
75713.2
66.5
L
32
558.9
L
37
—
—
—

LAB740
75265.3
53.8
0.28
6
—
—
—
—
—
—

LAB733
75158.2
—
—
—
—
—
—
11.4
0.03
5

LAB733
75158.5
—
—
—
518.8
0.20
27
11.8
0.21
8

LAB686
75364.6
56.2
0.08
11
—
—
—
—
—
—

LAB686
75365.2
—
—
—
—
—
—
11.9
0.18
9

LAB681
75418.2
—
—
—
—
—
—
11.8
0.24
9

LAB679
75629.1
—
—
—
492.0
0.06
21
—
—
—

LAB679
75629.4
—
—
—
480.4
L
18
11.2
0.28
3

LAB679
75631.1
—
—
—
537.5
0.27
32
12.3
0.01
13

LAB665
75040.3
57.5
0.13
14
518.8
0.28
27
—
—
—

LAB665
75042.3
60.4
0.12
20
504.5
0.15
24
—
—
—

LAB655
75119.1
60.7
0.04
20
500.0
0.01
23
11.6
0.23
7

LAB655
75119.2
—
—
—
443.8
0.25
9
—
—
—

LAB655
75122.1
59.4
0.13
18
525.0
0.05
29
11.5
0.16
6

LAB640
76268.1
—
—
—
—
—
—
11.1
0.26
2

CONT.
—
50.5
—
—
407.3
—
—
10.9
—
—

LAB815
76027.1
—
—
—
—
—
—
10.3
0.10
6

LAB815
76027.2
—
—
—
1042.9
0.29
8
10.3
0.10
6

LAB815
76029.4
—
—
—
—
—
—
10.2
0.22
6

LAB815
76030.1
103.1
0.26
15
1162.5
0.03
20
—
—
—

LAB794
75881.2
97.5
0.14
9
—
—
—
—
—
—

LAB794
75883.1
—
—
—
—
—
—
10.2
0.05
6

LAB794
75883.2
—
—
—
1071.4
0.28
10
—
—
—

LAB794
75884.1
—
—
—
1027.7
0.18
6
—
—
—

LAB794
75884.2
—
—
—
—
—
—
10.3
L
6

LAB774
76372.2
98.5
0.12
10
1069.6
0.20
10
—
—
—

LAB774
76375.1
103.1
0.05
15
—
—
—
—
—
—

LAB774
76375.3
101.9
0.02
13
—
—
—
10.0
0.22
3

LAB762
75866.2
—
—
—
—
—
—
10.2
0.22
6

LAB762
75869.2
—
—
—
1112.5
0.27
15
—
—
—

LAB762
75869.3
—
—
—
—
—
—
10.3
0.26
6

LAB748
74972.1
—
—
—
—
—
—
10.3
0.26
6

LAB748
74972.3
—
—
—
1037.5
0.18
7
10.4
0.16
7

LAB748
74972.4
—
—
—
—
—
—
10.5
0.25
8

LAB748
74976.2
—
—
—
—
—
—
10.7
L
10

LAB748
74977.2
—
—
—
—
—
—
10.4
0.16
7

LAB732
74966.2
—
—
—
—
—
—
10.4
0.02
7

LAB732
74967.1
—
—
—
1026.7
0.17
6
—
—
—

LAB732
74971.1
—
—
—
—
—
—
10.1
0.08
4

LAB732
74971.3
94.4
0.27
5
—
—
—
—
—
—

LAB732
74971.5
—
—
—
—
—
—
10.2
0.03
5

LAB712
76208.1
101.9
0.14
13
1100.0
0.03
13
—
—
—

LAB712
76208.3
—
—
—
—
—
—
10.4
0.02
7

LAB712
76208.4
104.4
0.04
16
1062.5
0.08
10
—
—
—

LAB712
76210.1
108.8
0.03
21
1118.8
L
15
—
—
—

LAB706
74800.1
106.9
0.29
19
1143.8
0.12
18
10.1
0.10
4

LAB695
74793.1
—
—
—
1043.8
0.19
8
10.0
0.22
3

LAB695
74793.2
—
—
—
—
—
—
10.1
0.26
4

LAB689
74759.2
—
—
—
—
—
—
9.9
0.23
2

LAB689
74760.1
97.5
0.14
9
1058.9
0.06
9
11.1
0.17
14

LAB683
74871.2
96.2
0.15
7
—
—
—
10.3
0.10
6

LAB683
74871.4
—
—
—
—
—
—
10.1
0.08
4

LAB683
74872.1
108.6
0.11
21
—
—
—
10.4
0.07
7

LAB683
74872.3
—
—
—
1035.7
0.27
7
—
—
—

LAB680
74774.1
—
—
—
1050.0
0.10
8
—
—
—

LAB680
74774.3
—
—
—
1112.5
L
15
10.8
L
11

LAB680
74774.4
103.8
0.27
16
1062.5
0.08
10
—
—
—

LAB680
74775.1
103.1
0.02
15
1025.0
0.26
6
—
—
—

LAB680
74777.2
—
—
—
—
—
—
10.2
0.03
5

LAB666
74840.2
—
—
—
—
—
—
10.6
0.21
9

LAB666
74840.3
—
—
—
—
—
—
10.6
L
9

LAB624
77108.1
—
—
—
1041.1
0.11
7
—
—
—

LAB624
77110.4
101.2
0.19
13
—
—
—
—
—
—

LAB619
77314.2
—
—
—
1050.0
0.12
8
10.0
0.22
3

CONT.
—
89.8
—
—
969.6
—
—
9.7
—
—

LAB837
76256.3
160.4
L
29
1680.4
0.11
22
—
—
—

LAB837
76256.5
150.6
L
21
1656.2
0.06
20
—
—
—

LAB837
76259.4
152.0
L
23
1750.0
L
27
9.8
0.08
13

LAB837
76259.6
139.2
0.08
12
—
—
—
—
—
—

LAB835
75896.4
132.4
0.27
7
—
—
—
—
—
—

LAB835
75896.5
138.1
0.07
11
—
—
—
9.4
0.15
8

LAB833
75465.1
151.2
0.20
22
—
—
—
—
—
—

LAB833
75466.1
152.5
0.04
23
—
—
—
9.4
L
8

LAB833
75467.3
137.8
0.22
11
—
—
—
9.2
0.19
7

LAB800
75331.5
—
—
—
—
—
—
9.4
0.20
9

LAB786
76226.1
—
—
—
—
—
—
9.1
0.01
6

LAB786
76229.1
—
—
—
—
—
—
8.9
0.13
3

LAB780
75772.2
134.4
0.21
8
—
—
—
—
—
—

LAB778
75966.2
150.0
0.24
21
—
—
—
9.1
0.02
5

LAB778
75966.3
140.0
0.16
13
1587.5
0.27
15
9.8
L
14

LAB778
75967.2
—
—
—
—
—
—
9.1
0.20
5

LAB778
75970.1
144.8
0.28
17
—
—
—
9.5
0.23
10

LAB776
75749.1
—
—
—
—
—
—
8.9
0.08
3

LAB776
75749.5
134.5
0.15
8
—
—
—
9.0
0.04
4

LAB776
75750.3
141.9
0.21
14
1487.5
0.18
8
—
—
—

LAB776
75750.4
—
—
—
—
—
—
9.4
0.15
8

LAB772
75461.3
—
—
—
—
—
—
8.9
0.28
3

LAB772
75461.6
—
—
—
—
—
—
9.1
0.20
5

LAB772
75462.5
135.0
0.22
9
—
—
—
9.1
0.01
6

LAB752
75165.2
139.9
0.03
13
—
—
—
—
—
—

LAB752
75168.1
143.8
0.25
16
1587.5
0.23
15
9.7
0.04
12

LAB752
75168.2
135.8
0.30
9
—
—
—
8.9
0.08
3

LAB752
75168.3
149.4
L
20
1525.0
0.08
10
—
—
—

LAB752
75170.1
140.5
0.14
13
—
—
—
—
—
—

LAB746
75507.6
—
—
—
—
—
—
9.2
0.13
6

LAB746
75507.8
—
—
—
—
—
—
9.5
0.23
10

LAB746
75508.2
—
—
—
—
—
—
8.8
0.28
2

LAB739
75442.1
142.9
0.26
15
1550.0
0.27
12
—
—
—

LAB739
75444.5
—
—
—
—
—
—
9.9
0.07
14

LAB733
75155.2
—
—
—
—
—
—
9.2
0.03
7

LAB733
75157.2
—
—
—
—
—
—
8.8
0.28
2

LAB718
75436.1
—
—
—
—
—
—
9.0
0.25
5

LAB718
75437.2
—
—
—
1525.0
0.15
10
—
—
—

LAB718
75439.2
139.4
0.05
12
—
—
—
—
—
—

LAB718
75439.3
147.5
0.20
19
—
—
—
—
—
—

LAB716
75431.2
167.5
L
35
1756.2
0.13
27
9.6
0.10
11

LAB681
75419.1
153.0
0.03
23
1596.4
0.04
15
9.1
0.22
5

LAB681
75421.2
—
—
—
—
—
—
9.1
0.02
5

LAB681
75421.4
—
—
—
—
—
—
9.6
0.05
11

LAB681
75421.5
138.5
0.06
12
—
—
—
—
—
—

CONT.
—
124.0
—
—
1382.9
—
—
8.6
—
—

LAB824
75504.1
126.3
0.10
15
—
—
—
—
—
—

LAB824
75505.7
120.9
0.10
10
—
—
—
—
—
—

LAB795
76491.3
123.1
0.19
12
1356.2
0.05
7
11.4
0.06
9

LAB795
76500.3
—
—
—
1375.0
0.14
8
—
—
—

LAB790
75713.2
—
—
—
1381.2
0.17
9
—
—
—

LAB789
77085.1
130.6
0.19
19
—
—
—
—
—
—

LAB784
76469.1
—
—
—
—
—
—
11.4
0.26
9

LAB769
75311.5
115.0
0.28
5
—
—
—
—
—
—

LAB730
75180.3
130.9
0.02
19
—
—
—
—
—
—

LAB713
75090.2
120.6
0.05
10
—
—
—
—
—
—

LAB713
75092.4
120.6
0.05
10
—
—
—
—
—
—

LAB708
75340.2
116.9
0.24
6
—
—
—
—
—
—

LAB649
77042.4
—
—
—
1477.7
0.15
16
—
—
—

LAB649
77044.2
130.0
0.04
18
—
—
—
—
—
—

LAB636
75100.3
—
—
—
1343.8
0.05
6
11.4
0.20
9

LAB636
75104.1
128.8
L
17
1400.0
L
10
11.3
0.07
8

LAB618
77782.2
—
—
—
—
—
—
11.2
0.09
8

LAB618
77784.2
128.1
0.05
17
1331.2
0.13
5
11.4
0.10
9

CONT.
—
109.9
—
—
1269.9
—
—
10.5
—
—

LAB848
77339.2
—
—
—
—
—
—
11.7
0.19
11

LAB842
76348.1
—
—
—
—
—
—
11.2
0.13
7

LAB842
76350.2
—
—
—
—
—
—
10.9
0.19
4

LAB841
76803.1
—
—
—
475.0
0.09
23
11.4
0.03
9

LAB832
77332.4
—
—
—
425.0
0.14
10
—
—
—

LAB829
76442.2
—
—
—
—
—
—
11.2
0.22
6

LAB827
76795.5
—
—
—
—
—
—
10.9
0.15
3

LAB816
76719.1
53.1
0.26
31
—
—
—
—
—
—

LAB803
76760.1
52.1
0.04
29
500.0
L
29
11.0
0.07
5

LAB803
76760.2
—
—
—
—
—
—
11.0
0.03
5

LAB779
77306.4
48.3
0.30
19
432.1
0.16
12
—
—
—

LAB770
77765.2
—
—
—
443.8
0.27
15
—
—
—

LAB767
77701.1
45.7
0.28
13
421.4
0.13
9
—
—
—

LAB767
77705.1
—
—
—
—
—
—
10.8
0.16
3

LAB765
76113.3
—
—
—
—
—
—
10.9
0.15
3

LAB764
76107.5
—
—
—
—
—
—
11.2
0.13
7

LAB764
76110.5
—
—
—
—
—
—
11.2
0.01
7

LAB758
76490.4
—
—
—
469.6
0.28
21
11.9
0.21
14

LAB757
77326.3
—
—
—
437.5
0.06
13
11.2
L
6

LAB757
77329.1
51.0
0.20
26
433.0
0.04
12
—
—
—

LAB753
76439.3
—
—
—
—
—
—
10.8
0.16
3

LAB753
76440.2
46.2
0.24
14
—
—
—
—
—
—

LAB751
76929.2
—
—
—
—
—
—
11.4
L
8

LAB749
77757.4
50.6
0.07
25
456.2
0.07
18
—
—
—

LAB742
76434.3
—
—
—
—
—
—
11.3
0.29
8

LAB735
77323.1
—
—
—
—
—
—
11.1
0.02
5

LAB735
77324.4
—
—
—
—
—
—
10.9
0.05
4

LAB731
77133.1
—
—
—
433.0
0.04
12
—
—
—

LAB717
76430.1
—
—
—
—
—
—
11.8
0.05
12

LAB703
76422.2
—
—
—
—
—
—
10.9
0.15
3

LAB691
74784.2
59.4
0.20
47
—
—
—
11.4
0.03
9

LAB691
74785.4
—
—
—
—
—
—
11.1
0.17
6

LAB690
77276.2
—
—
—
—
—
—
11.2
0.22
6

LAB678
77823.3
47.1
0.18
16
—
—
—
—
—
—

LAB676
77275.2
—
—
—
—
—
—
11.0
0.03
5

LAB671
76411.1
49.4
0.10
22
487.5
0.17
26
11.5
0.07
9

LAB671
76411.6
—
—
—
—
—
—
10.9
0.15
3

LAB669_H7
77879.4
—
—
—
—
—
—
11.1
0.12
5

LAB667
76097.3
—
—
—
—
—
—
11.3
0.29
8

LAB664
76224.4
—
—
—
—
—
—
10.9
0.15
3

LAB663
77047.2
—
—
—
456.2
L
18
—
—
—

LAB656
77316.1
—
—
—
449.1
0.30
16
11.4
L
8

LAB641
77221.2
—
—
—
—
—
—
11.9
L
13

LAB641
77224.1
46.2
0.24
14
—
—
—
—
—
—

LAB641
77225.2
55.6
0.26
38
562.5
0.14
45
12.0
L
14

LAB633
74831.4
—
—
—
—
—
—
11.0
0.03
5

LAB630
77786.1
—
—
—
—
—
—
11.4
0.09
8

LAB629
76896.2
—
—
—
462.5
0.01
19
11.1
0.28
5

LAB622
75036.3
—
—
—
426.8
0.07
10
—
—
—

CONT.
—
40.4
—
—
387.1
—
—
10.5
—
—

LAB840
75832.5
129.1
0.02
18
1314.3
0.15
8
—
—
—

LAB840
75833.1
138.8
0.02
26
1393.8
0.16
14
—
—
—

LAB840
75833.2
123.1
0.08
12
—
—
—
—
—
—

LAB840
75834.2
126.8
0.02
15
—
—
—
—
—
—

LAB839
75624.1
—
—
—
1393.8
0.26
14
—
—
—

LAB830
76057.3
123.8
0.04
13
—
—
—
12.1
0.02
6

LAB830
76060.4
139.4
L
27
1356.2
0.11
11
—
—
—

LAB815
76029.2
148.1
L
35
1475.0
0.28
21
—
—
—

LAB804
76281.3
125.0
0.23
14
1368.8
0.06
12
—
—
—

LAB804
76282.1
133.8
L
22
1406.2
0.14
15
—
—
—

LAB801
75886.5
—
—
—
—
—
—
11.9
0.26
4

LAB801
75886.7
120.6
0.13
10
—
—
—
—
—
—

LAB801
75889.1
130.7
0.10
19
1357.1
0.11
11
—
—
—

LAB800
75331.2
125.0
0.23
14
1393.8
0.10
14
—
—
—

LAB794
75881.2
123.1
0.08
12
—
—
—
—
—
—

LAB794
75884.2
145.0
L
32
1300.0
0.21
7
—
—
—

LAB788
76232.1
138.8
0.08
26
1475.0
0.25
21
—
—
—

LAB788
76233.1
146.2
0.22
33
—
—
—
—
—
—

LAB786
76230.2
131.9
0.03
20
1375.0
0.22
13
12.0
0.05
5

LAB786
76230.7
123.8
0.20
13
—
—
—
—
—
—

LAB777
76272.2
130.6
0.07
19
—
—
—
—
—
—

LAB777
76273.1
132.5
L
21
1333.0
0.09
9
—
—
—

LAB777
76273.3
148.8
0.02
35
—
—
—
—
—
—

LAB741
75609.1
126.2
0.03
15
1406.2
0.28
15
—
—
—

LAB741
75609.3
123.8
0.05
13
—
—
—
—
—
—

LAB741
75611.4
130.4
0.04
19
1390.2
0.07
14
—
—
—

LAB741
75612.1
119.4
0.13
9
—
—
—
—
—
—

LAB712
76208.4
118.1
0.29
8
—
—
—
—
—
—

LAB704
75132.1
136.2
0.27
24
1350.0
0.07
11
—
—
—

LAB704
75132.2
141.9
0.24
29
1456.2
0.02
20
—
—
—

LAB704
75132.3
117.9
0.22
7
—
—
—
—
—
—

LAB704
75133.1
121.9
0.10
11
—
—
—
—
—
—

LAB665
75039.2
118.8
0.30
8
—
—
—
—
—
—

LAB665
75040.3
126.7
0.02
15
—
—
—
—
—
—

CONT.
—
109.8
—
—
1217.9
—
—
11.4
—
—

LAB835
75896.4
—
—
—
—
—
—
10.8
0.10
5

LAB835
75896.5
111.5
0.30
33
—
—
—
—
—
—

LAB833
75465.1
109.4
0.12
30
962.5
0.02
21
—
—
—

LAB833
75468.4
92.5
0.20
10
1062.5
0.13
33
—
—
—

LAB796
75321.2
100.5
0.11
20
1028.3
L
29
—
—
—

LAB796
75322.1
111.9
L
33
987.5
0.01
24
—
—
—

LAB796
75324.1
99.4
0.08
18
893.8
0.22
12
—
—
—

LAB780
75771.2
99.4
0.25
18
968.8
0.03
22
10.9
0.09
5

LAB780
75771.3
111.2
L
32
1012.5
L
27
—
—
—

LAB780
75774.1
—
—
—
—
—
—
10.9
0.24
6

LAB778
75967.2
114.0
L
36
1089.3
0.04
37
—
—
—

LAB778
75969.2
103.8
0.25
24
—
—
—
10.9
0.24
6

LAB778
75969.4
100.8
0.02
20
958.0
0.19
20
—
—
—

LAB778
75970.1
105.6
L
26
1018.8
L
28
—
—
—

LAB776
75746.2
104.4
0.04
24
1056.2
0.04
33
11.3
0.02
10

LAB776
75749.1
106.9
L
27
1006.3
0.03
26
—
—
—

LAB776
75750.3
109.4
L
30
1012.5
0.09
27
—
—
—

LAB776
75750.4
108.1
0.13
29
1106.2
0.12
39
—
—
—

LAB772
75461.6
97.1
0.26
16
977.7
0.14
23
—
—
—

LAB772
75462.5
95.4
0.08
14
957.1
0.13
20
—
—
—

LAB768
75453.1
96.9
0.06
15
906.2
0.09
14
—
—
—

LAB768
75453.2
—
—
—
—
—
—
10.8
0.17
4

LAB768
75453.3
100.1
0.26
19
—
—
—
—
—
—

LAB768
75455.1
108.1
L
29
1006.3
0.17
26
—
—
—

LAB752
75165.2
101.9
0.02
21
943.8
0.03
18
—
—
—

LAB752
75168.1
103.4
0.01
23
969.6
0.10
22
11.0
0.14
7

LAB752
75168.2
—
—
—
900.0
0.23
13
—
—
—

LAB752
75170.1
105.0
0.23
25
—
—
—
—
—
—

LAB746
75507.2
90.6
0.27
8
868.8
0.28
9
—
—
—

LAB746
75508.3
—
—
—
862.5
0.26
8
—
—
—

LAB739
75441.2
100.6
0.02
20
887.5
0.14
11
—
—
—

LAB739
75442.3
—
—
—
881.2
0.28
11
—
—
—

LAB739
75444.4
91.9
0.20
9
—
—
—
—
—
—

LAB739
75445.1
—
—
—
—
—
—
10.7
0.29
4

LAB733
75154.3
101.9
0.02
21
—
—
—
—
—
—

LAB733
75155.2
94.9
0.15
13
—
—
—
—
—
—

LAB724
75143.2
—
—
—
—
—
—
10.7
0.29
4

LAB724
75146.2
—
—
—
987.5
0.03
24
—
—
—

LAB718
75435.2
92.5
0.17
10
918.8
0.09
15
—
—
—

LAB718
75436.1
100.0
0.27
19
900.0
0.12
13
—
—
—

LAB716
75430.1
91.9
0.25
9
887.5
0.14
11
—
—
—

LAB716
75430.5
109.3
L
30
1128.6
L
42
10.7
0.17
4

LAB705
74879.2
113.8
0.11
35
—
—
—
10.8
0.13
4

LAB705
74879.3
107.5
0.20
28
950.0
0.23
19
—
—
—

LAB705
74881.2
101.9
0.05
21
887.5
0.28
11
—
—
—

LAB686
75364.4
121.1
0.02
44
1090.2
0.01
37
10.8
0.10
5

LAB681
75419.1
—
—
—
861.2
0.26
8
—
—
—

CONT.
—
84.0
—
—
796.9
—
—
10.3
—
—

LAB791
75319.5
—
—
—
—
—
—
10.4
0.13
4

LAB769
75311.3
135.6
0.26
20
—
—
—
10.2
0.29
3

LAB755
75175.1
—
—
—
—
—
—
10.3
0.29
3

LAB750
75448.2
—
—
—
—
—
—
10.4
0.18
5

LAB704
75132.2
—
—
—
—
—
—
10.6
0.11
6

LAB670
75306.3
—
—
—
—
—
—
10.5
0.08
5

LAB653
75113.4
—
—
—
—
—
—
10.7
0.05
7

LAB623
75096.2
—
—
—
—
—
—
10.4
0.13
4

CONT.
—
113.5
—
—
—
—
—
10.0
—
—

LAB834
75892.5
—
—
—
—
—
—
10.2
0.04
4

LAB834
75895.3
123.9
0.15
38
1183.9
0.01
27
10.4
0.17
6

LAB834
75895.5
110.8
0.03
23
—
—
—
—
—
—

LAB825
76394.1
—
—
—
—
—
—
10.4
L
7

LAB825
76394.2
104.0
0.06
16
—
—
—
—
—
—

LAB825
76395.2
111.3
L
24
—
—
—
—
—
—

LAB793
75751.1
—
—
—
—
—
—
10.1
0.13
3

LAB793
75751.3
105.6
0.25
17
—
—
—
10.4
0.22
7

LAB793
75755.1
112.5
0.29
25
—
—
—
—
—
—

LAB793
75755.3
106.2
0.09
18
—
—
—
10.4
L
7

LAB787
76753.1
100.7
0.30
12
—
—
—
—
—
—

LAB787
76754.1
115.3
0.15
28
1139.3
L
23
—
—
—

LAB760
75862.2
104.4
0.04
16
1068.8
0.11
15
10.4
0.17
6

LAB760
75863.1
—
—
—
—
—
—
10.7
0.01
10

LAB760
75863.2
114.1
0.14
27
1289.3
L
39
10.7
0.03
10

LAB760
75865.2
—
—
—
—
—
—
10.8
L
10

LAB760
75865.3
—
—
—
—
—
—
10.2
0.02
5

LAB736
76368.1
—
—
—
1093.8
0.02
18
10.6
0.18
8

LAB736
76368.2
110.6
0.26
23
1168.8
L
26
10.8
0.27
10

LAB736
76370.1
—
—
—
—
—
—
10.4
0.22
7

LAB736
76370.4
120.7
L
34
—
—
—
10.4
0.17
6

LAB727
76387.3
—
—
—
1212.5
L
31
10.7
L
10

LAB727
76389.2
103.8
0.26
15
—
—
—
10.4
L
6

LAB726
76381.1
112.6
0.17
25
—
—
—
10.1
0.06
4

LAB726
76382.1
—
—
—
—
—
—
10.4
0.07
7

LAB726
76385.2
—
—
—
—
—
—
10.4
0.17
6

LAB726
76385.3
111.9
0.24
24
1100.0
0.02
18
—
—
—

LAB707
75855.5
116.5
0.10
30
1001.8
0.27
8
—
—
—

LAB698
76201.1
100.0
0.11
11
—
—
—
—
—
—

LAB698
76202.2
104.4
0.15
16
1037.5
0.22
12
10.1
0.13
3

LAB698
76204.1
—
—
—
1031.2
0.28
11
—
—
—

LAB688
75829.1
—
—
—
—
—
—
10.5
0.13
8

LAB687
75045.2
—
—
—
—
—
—
10.4
L
7

LAB687
75047.2
114.1
0.14
27
—
—
—
11.5
L
18

LAB677
76521.3
115.7
0.04
29
1085.7
0.04
17
—
—
—

LAB677
76522.2
140.6
0.18
56
1212.5
0.12
31
—
—
—

LAB673
76537.1
—
—
—
1142.0
0.01
23
10.4
L
7

LAB650
75838.4
—
—
—
—
—
—
10.5
0.02
8

LAB639
76504.1
112.5
L
25
—
—
—
—
—
—

LAB639
76504.3
—
—
—
1325.0
0.10
43
10.6
L
9

LAB639
76505.1
97.9
0.19
9
—
—
—
—
—
—

LAB639
76510.1
—
—
—
1131.2
L
22
10.0
0.29
3

LAB625
76452.1
121.9
0.21
35
1231.2
0.03
33
—
—
—

LAB625
76452.2
—
—
—
—
—
—
10.3
0.11
6

LAB625
76452.5
—
—
—
—
—
—
10.8
0.18
10

LAB625
76472.1
—
—
—
—
—
—
10.9
0.16
12

CONT.
—
89.9
—
—
929.1
—
—
9.8
—
—

“CONT ”—Control;

“Ave.”—Average;

“% Incr.” = % increment;

“p-val.”—p-value,

L—p < 0.01.

TABLE 165

Genes showing improved plant performance at drought growth

conditions under regulation of At6669 promoter

Rosette Diameter

Gene

Plot Coverage [cm²]
Rosette Area [cm²]
[cm]

Name
Event #
Ave.
P-Val.
% Incr.
Ave.
P-Val.
% Incr.
Ave.
P-Val.
% Incr.

LAB795
76491.3
77.4
0.02
41
9.7
0.02
41
5.2
0.09
19

LAB795
76494.3
66.4
0.29
21
8.3
0.29
21
—
—
—

LAB792
75876.3
68.8
0.08
26
8.6
0.08
26
4.9
0.07
13

LAB790
75713.2
71.4
0.07
31
8.9
0.07
31
4.9
0.12
11

LAB789
77081.1
—
—
—
—
—
—
4.7
0.22
8

LAB789
77085.3
63.2
0.26
16
7.9
0.26
16
—
—
—

LAB784
76467.4
76.0
0.19
39
9.5
0.19
39
5.2
0.18
19

LAB769
75309.1
77.4
0.02
42
9.7
0.02
42
5.1
0.03
18

LAB750
75447.2
71.8
0.04
31
9.0
0.04
31
5.1
0.03
17

LAB730
75180.2
68.4
0.10
25
8.5
0.10
25
4.9
0.17
11

LAB729
77303.7
72.0
0.09
32
9.0
0.09
32
5.0
0.08
13

LAB713
75092.1
71.3
0.05
30
8.9
0.05
30
4.9
0.09
12

LAB708
75288.5
—
—
—
—
—
—
4.7
0.23
8

LAB670
75303.1
68.5
0.08
25
8.6
0.08
25
5.0
0.06
14

LAB656
77320.6
82.3
0.24
51
10.3
0.24
51
5.3
0.20
21

LAB649
77041.3
65.4
0.28
20
8.2
0.28
20
—
—
—

LAB649
77044.4
66.3
0.15
21
8.3
0.15
21
4.8
0.23
9

LAB649
77045.3
65.9
0.14
21
8.2
0.14
21
4.8
0.13
10

LAB637
75105.8
68.3
0.09
25
8.5
0.09
25
4.8
0.13
10

LAB636
75100.3
81.8
0.02
50
10.2
0.02
50
5.4
L
24

LAB636
75104.8
74.6
0.02
37
9.3
0.02
37
5.1
0.03
17

CONT.
—
54.7
—
—
6.8
—
—
4.4
—
—

LAB839
75624.1
114.6
0.05
22
14.3
0.05
22
6.2
0.19
11

LAB809
75653.2
111.4
0.25
19
13.9
0.25
19
—
—
—

LAB805
76117.2
106.4
0.17
13
13.3
0.17
13
5.9
0.17
5

LAB798
75615.4
—
—
—
—
—
—
5.9
L
5

LAB798
75616.2
107.8
0.11
15
13.5
0.11
15
6.0
0.16
7

LAB792
75876.1
100.9
0.06
7
12.6
0.06
7
6.0
0.02
8

LAB790
75713.2
125.5
0.24
34
15.7
0.24
34
6.4
0.16
15

LAB740
75265.2
97.5
0.27
4
12.2
0.27
4
5.8
0.05
4

LAB740
75265.3
107.8
0.11
15
13.5
0.11
15
—
—
—

LAB733
75158.5
124.4
0.18
33
15.5
0.18
33
6.2
0.16
11

LAB681
75418.2
114.6
0.21
22
14.3
0.21
22
6.1
0.05
9

LAB679
75629.1
112.2
0.30
20
14.9
L
27
6.3
0.08
13

LAB679
75629.4
99.3
0.13
6
12.4
0.13
6
—
—
—

LAB679
75631.1
129.7
0.13
38
16.2
0.13
38
6.2
0.11
12

LAB665
75042.3
110.4
0.27
18
13.8
0.27
18
6.2
0.16
10

LAB664
76224.3
99.1
0.19
6
12.4
0.19
6
5.7
0.14
2

LAB655
75119.1
111.5
0.29
19
14.9
L
27
6.1
L
9

LAB655
75122.1
118.5
L
26
14.8
L
26
6.2
0.11
11

LAB640
76267.4
—
—
—
—
—
—
5.8
0.23
5

CONT.
—
93.8
—
—
11.7
—
—
5.6
—
—

LAB815
76030.1
73.9
0.12
11
9.2
0.12
11
5.0
0.07
6

LAB794
75884.1
81.2
L
21
10.1
L
21
5.2
0.02
10

LAB762
75869.2
—
—
—
—
—
—
5.0
0.30
5

LAB762
75869.3
74.0
0.12
11
9.2
0.12
11
4.9
0.27
4

LAB762
75870.2
71.6
0.28
7
9.0
0.28
7
4.9
0.21
4

LAB748
74972.1
76.6
0.22
15
9.6
0.22
15
—
—
—

LAB748
74972.3
74.6
0.22
12
9.3
0.22
12
—
—
—

LAB748
74976.2
73.7
0.24
10
9.2
0.24
10
—
—
—

LAB732
74966.2
73.0
0.25
9
9.1
0.25
9
—
—
—

LAB732
74969.1
75.8
0.17
13
9.5
0.17
13
5.0
0.10
6

LAB732
74971.3
73.8
0.28
10
9.2
0.28
10
—
—
—

LAB732
74971.5
80.9
0.01
21
10.1
0.01
21
5.2
L
11

LAB706
74799.1
75.0
0.12
12
9.4
0.12
12
5.0
0.06
6

LAB706
74800.1
72.3
0.22
8
9.0
0.22
8
4.9
0.25
3

LAB695
74797.4
—
—
—
—
—
—
4.9
0.22
4

LAB689
74756.3
72.9
0.17
9
9.1
0.17
9
5.0
0.13
5

LAB689
74758.4
76.9
0.04
15
9.6
0.04
15
4.9
0.26
3

LAB689
74760.1
81.9
L
23
10.2
L
23
5.2
0.02
9

LAB683
74872.1
72.3
0.22
8
9.0
0.22
8
—
—
—

LAB683
74872.3
76.0
0.17
14
9.5
0.17
14
5.0
0.19
6

LAB683
74873.1
77.6
0.11
16
9.7
0.11
16
5.0
0.06
7

LAB680
74774.3
83.1
L
24
10.4
L
24
5.2
0.01
9

LAB680
74774.4
78.4
0.02
17
9.8
0.02
17
5.1
0.03
8

LAB680
74775.1
77.3
0.10
16
9.7
0.10
16
4.9
0.24
4

LAB680
74777.1
—
—
—
—
—
—
4.9
0.19
4

LAB680
74777.2
73.0
0.26
9
9.1
0.26
9
5.0
0.06
7

LAB666
74845.1
75.7
0.12
13
9.5
0.12
13
5.0
0.12
5

LAB619
77311.1
74.3
0.15
11
9.3
0.15
11
5.0
0.08
6

LAB619
77314.2
—
—
—
—
—
—
4.9
0.24
4

CONT.
—
66.9
—
—
8.4
—
—
4.7
—
—

LAB837
76256.3
71.7
0.04
39
9.0
0.04
39
5.1
0.04
18

LAB837
76256.5
61.2
0.13
19
7.7
0.13
19
4.8
0.17
11

LAB837
76259.4
68.3
0.18
32
8.5
0.18
32
5.2
0.17
18

LAB835
75896.5
56.8
0.18
10
7.1
0.18
10
4.7
0.04
8

LAB835
75900.1
—
—
—
—
—
—
4.6
0.08
5

LAB833
75465.1
62.2
0.28
21
7.8
0.28
21
4.9
0.09
12

LAB833
75466.1
57.5
0.21
11
7.2
0.21
11
—
—
—

LAB800
75331.5
—
—
—
—
—
—
4.9
0.11
11

LAB786
76230.2
57.9
0.01
12
7.2
0.01
12
4.8
L
9

LAB778
75966.2
—
—
—
—
—
—
4.6
0.26
5

LAB778
75966.3
65.9
L
28
8.2
L
28
4.9
0.01
11

LAB776
75750.3
56.0
0.06
9
7.0
0.06
9
4.7
0.02
7

LAB772
75462.2
—
—
—
6.9
0.09
7
4.7
0.11
9

LAB768
75453.1
—
—
—
—
—
—
4.6
0.06
5

LAB768
75453.2
—
—
—
—
—
—
4.6
0.11
5

LAB752
75165.2
—
—
—
—
—
—
4.6
0.19
4

LAB752
75168.1
62.4
0.07
21
7.8
0.07
21
4.8
0.06
11

LAB752
75168.3
62.7
0.06
22
7.8
0.06
22
4.7
0.01
8

LAB746
75507.6
54.3
0.19
5
6.8
0.19
5
4.6
0.24
5

LAB746
75507.8
62.5
0.17
21
7.8
0.17
21
4.8
0.05
11

LAB739
75444.4
55.3
0.11
7
6.9
0.11
7
—
—
—

LAB739
75444.5
58.6
0.09
14
7.3
0.09
14
4.7
0.07
7

LAB718
75437.2
54.9
0.19
6
6.9
0.19
6
4.6
0.13
5

LAB718
75439.3
65.7
0.23
28
8.2
0.23
28
4.9
0.16
11

LAB716
75429.3
56.2
0.26
9
7.0
0.26
9
4.7
0.28
8

LAB716
75431.2
65.4
L
27
8.2
L
27
5.1
0.08
17

LAB686
75364.1
—
—
—
—
—
—
4.6
0.10
4

LAB681
75419.1
—
—
—
7.3
L
13
4.7
0.11
8

LAB681
75421.4
55.1
0.10
7
6.9
0.10
7
4.6
0.12
5

CONT.
—
51.6
—
—
6.4
—
—
4.4
—
—

LAB824
75504.1
88.8
0.10
10
11.1
0.10
10
5.5
0.16
5

LAB792
75876.3
91.6
0.04
13
11.5
0.04
13
5.6
0.08
6

LAB636
75100.3
86.7
0.21
7
10.8
0.21
7
5.5
0.27
4

LAB636
75104.1
100.3
0.01
24
12.5
0.01
24
5.9
0.03
12

LAB618
77784.2
—
—
—
—
—
—
5.6
0.09
7

CONT.
—
80.9
—
—
10.1
—
—
5.3
—
—

LAB850
77714.3
71.0
0.04
9
8.9
0.14
8
5.0
0.13
7

LAB848
77336.7
70.8
0.03
9
8.8
0.14
7
5.0
0.08
6

LAB844
76787.4
—
—
—
—
—
—
4.9
0.27
5

LAB842
76350.2
75.3
0.14
16
9.4
0.15
14
5.1
0.24
8

LAB842
76350.3
74.7
L
15
9.3
0.02
13
5.2
0.02
10

LAB841
76803.1
82.3
L
27
10.3
L
25
5.3
L
14

LAB832
77332.4
79.2
0.05
22
9.9
0.04
20
5.2
L
11

LAB827
76791.2
78.3
0.18
21
9.8
0.18
19
5.2
0.11
10

LAB827
76794.3
69.5
0.08
7
8.7
0.27
5
5.0
0.12
6

LAB823
77026.4
75.6
0.25
17
9.5
0.27
15
5.1
0.14
9

LAB823
77027.1
73.5
0.28
13
—
—
—
5.2
0.15
12

LAB823
77028.2
—
—
—
—
—
—
4.9
0.20
4

LAB816
76717.2
—
—
—
—
—
—
4.9
0.25
4

LAB816
76719.1
70.5
0.04
9
8.8
0.16
7
4.8
0.25
3

LAB811
76343.1
69.8
0.12
8
8.7
0.28
6
5.0
0.05
8

LAB803
76760.1
75.7
0.09
17
9.5
0.10
15
5.0
0.02
8

LAB803
76760.2
78.6
0.04
21
9.8
0.04
19
5.3
0.21
14

LAB770
77765.2
80.2
0.23
24
10.0
0.23
22
5.2
0.06
12

LAB767
77701.1
—
—
—
—
—
—
5.0
0.05
6

LAB765
76113.3
72.5
0.09
12
9.1
0.14
10
—
—
—

LAB765
76113.5
—
—
—
—
—
—
4.9
0.23
4

LAB764
76107.5
72.6
0.09
12
9.1
0.14
10
5.0
0.23
7

LAB764
76110.1
—
—
—
—
—
—
4.9
0.21
4

LAB758
76490.4
85.7
L
32
10.7
L
30
5.4
0.02
15

LAB757
77326.3
82.9
L
28
10.4
L
26
5.4
0.06
15

LAB757
77326.7
79.4
L
22
9.9
L
20
5.2
0.02
10

LAB757
77329.1
73.7
0.24
14
9.2
0.27
12
5.0
0.15
7

LAB753
76439.3
—
—
—
—
—
—
5.0
0.28
7

LAB753
76440.2
77.1
L
19
9.6
L
17
5.2
L
10

LAB751
76929.2
82.0
0.01
26
10.2
L
24
5.2
L
12

LAB749
77757.4
78.4
0.03
21
9.8
0.03
19
5.3
0.04
13

LAB742
76432.1
75.9
0.27
17
9.5
0.29
15
5.0
0.25
7

LAB742
76434.3
—
—
—
—
—
—
4.9
0.13
5

LAB722
76464.1
74.2
L
14
9.3
0.02
12
5.1
0.05
8

LAB722
76465.2
77.9
0.28
20
9.7
0.29
18
5.2
0.27
12

LAB717
76430.1
83.3
0.26
28
10.4
0.26
26
5.2
0.26
12

LAB711
77076.1
85.3
0.06
31
10.7
0.05
29
5.3
L
14

LAB711
77077.3
—
—
—
—
—
—
5.0
0.10
7

LAB692
76418.1
68.7
0.14
6
—
—
—
4.9
0.11
5

LAB691
74784.2
74.2
0.16
14
9.3
0.18
13
5.1
0.13
8

LAB691
74785.4
72.8
0.24
12
9.1
0.28
10
5.1
0.06
9

LAB690
77276.2
77.4
0.07
19
9.7
0.07
17
5.3
0.05
13

LAB672
77699.2
73.3
0.04
13
9.2
0.07
11
5.1
0.05
10

LAB672
77700.1
68.3
0.22
5
—
—
—
—
—
—

LAB671
76411.1
87.0
0.04
34
10.9
0.03
32
5.5
L
18

LAB671
76411.6
70.1
0.07
8
8.8
0.22
6
—
—
—

LAB664
76224.4
71.2
0.03
10
8.9
0.11
8
5.0
0.10
6

LAB663
77046.1
73.4
0.17
13
9.2
0.20
11
—
—
—

LAB663
77047.2
77.0
L
19
9.6
L
17
5.2
L
10

LAB663
77048.1
69.0
0.12
6
—
—
—
4.9
0.15
5

LAB662
77296.3
71.9
0.03
11
9.0
0.10
9
5.0
0.03
7

LAB656
77316.1
80.2
0.01
24
10.0
0.01
22
5.2
0.15
11

LAB656
77318.2
73.9
0.08
14
9.2
0.11
12
5.0
0.05
7

LAB653
75113.1
71.1
0.15
10
8.9
0.23
8
5.1
0.01
9

LAB641
77224.1
78.7
L
21
9.8
L
19
5.1
L
10

LAB641
77225.2
101.0
0.11
56
12.6
0.10
53
5.9
0.09
25

LAB633
74828.2
67.7
0.26
4
—
—
—
4.8
0.29
3

LAB633
74831.4
74.2
0.30
14
—
—
—
5.1
0.23
10

LAB633
74832.1
—
—
—
—
—
—
5.0
0.27
8

LAB629
76896.2
86.5
0.08
33
10.8
0.07
31
5.4
0.03
16

LAB627
77694.2
70.5
0.09
9
8.8
0.21
7
—
—
—

LAB622
75036.3
82.4
0.23
27
10.3
0.24
25
5.3
0.06
14

CONT.
—
64.9
—
—
8.2
—
—
4.7
—
—

LAB840
75833.2
107.7
0.28
18
13.5
0.28
18
5.9
0.18
8

LAB830
76056.1
98.3
0.30
8
12.3
0.30
8
5.8
0.25
6

LAB815
76029.4
110.2
0.04
21
13.8
0.04
21
6.0
0.02
10

LAB804
76281.3
103.4
0.10
14
12.9
0.10
14
5.9
0.10
8

LAB804
76282.1
111.1
0.14
22
13.9
0.14
22
6.1
0.13
12

LAB788
76232.1
101.2
0.28
11
12.6
0.28
11
6.0
0.27
10

LAB777
76274.7
118.9
0.10
31
14.9
0.10
31
6.1
0.25
13

LAB704
75132.2
—
—
—
—
—
—
5.7
0.28
5

CONT.
—
91.0
—
—
11.4
—
—
5.4
—
—

LAB835
75896.3
—
—
—
—
—
—
4.8
0.11
4

LAB833
75465.1
64.8
0.30
21
8.1
0.30
21
5.1
0.08
10

LAB833
75466.1
—
—
—
—
—
—
4.9
0.17
5

LAB833
75467.4
57.2
0.24
7
7.1
0.24
7
—
—
—

LAB796
75322.1
66.6
0.15
25
8.3
0.15
25
5.2
0.07
11

LAB796
75323.2
62.8
L
18
7.9
L
18
5.0
0.02
8

LAB796
75324.1
63.1
L
18
7.9
L
18
5.0
L
8

LAB780
75771.2
67.1
L
26
8.4
L
26
5.2
L
13

LAB780
75771.3
72.4
0.08
36
9.1
0.08
36
5.4
0.04
16

LAB780
75773.3
63.8
0.22
19
8.0
0.22
19
5.0
L
8

LAB780
75774.1
58.3
0.10
9
7.3
0.10
9
—
—
—

LAB778
75967.2
76.5
0.12
43
9.6
0.12
43
5.5
L
19

LAB778
75969.2
68.1
0.02
27
8.5
0.02
27
5.2
0.27
13

LAB778
75969.4
66.3
L
24
8.3
L
24
5.1
L
10

LAB776
75746.2
70.4
0.16
32
8.8
0.16
32
5.2
0.28
11

LAB776
75750.3
66.2
L
24
8.3
L
24
5.1
L
10

LAB776
75750.4
66.3
L
24
8.3
L
24
5.1
L
10

LAB772
75459.1
56.5
0.26
6
7.1
0.26
6
4.9
0.06
6

LAB768
75453.1
61.9
0.11
16
7.7
0.11
16
4.9
0.13
5

LAB768
75453.2
64.4
0.24
21
8.1
0.24
21
—
—
—

LAB768
75455.1
67.0
0.19
25
8.4
0.19
25
5.2
0.24
13

LAB752
75168.1
64.6
L
21
8.1
L
21
4.9
0.01
6

LAB746
75507.2
58.1
0.08
9
7.3
0.08
9
—
—
—

LAB739
75441.2
63.0
0.27
18
7.9
0.27
18
—
—
—

LAB739
75444.4
59.4
0.15
11
7.4
0.15
11
4.9
0.27
5

LAB739
75445.1
65.1
L
22
8.1
L
22
5.1
L
10

LAB733
75158.2
59.2
0.27
11
7.4
0.27
11
—
—
—

LAB724
75142.1
68.1
0.02
28
8.5
0.02
28
5.2
L
12

LAB724
75143.2
63.0
L
18
7.9
L
18
5.1
L
9

LAB718
75435.2
58.3
0.12
9
7.3
0.12
9
—
—
—

LAB718
75436.1
—
—
—
—
—
—
5.2
0.28
11

LAB718
75437.2
60.0
0.07
12
7.5
0.07
12
4.8
0.05
4

LAB718
75439.2
58.9
0.04
10
7.4
0.04
10
—
—
—

LAB716
75429.3
62.4
0.04
17
7.8
0.04
17
5.0
0.03
7

LAB716
75430.5
65.9
0.21
23
8.8
L
32
5.4
L
16

LAB705
74879.2
72.2
L
35
9.0
L
35
5.4
L
16

LAB705
74879.3
65.5
0.23
23
8.2
0.23
23
5.1
0.05
9

LAB705
74881.1
63.7
0.18
19
8.0
0.18
19
5.1
0.07
10

LAB686
75364.4
68.7
0.02
29
8.6
0.02
29
5.2
L
12

CONT.
—
53.4
—
—
6.7
—
—
4.6
—
—

LAB791
75318.2
—
—
—
—
—
—
5.3
0.30
13

LAB670
75306.3
78.8
0.21
30
9.8
0.21
30
5.3
0.27
13

CONT.
—
60.5
—
—
7.6
—
—
4.7
—
—

LAB834
75895.3
91.7
L
30
11.5
L
30
5.8
L
14

LAB807
76240.1
76.9
0.29
9
9.6
0.29
9
—
—
—

LAB793
75755.1
78.6
0.17
11
9.8
0.17
11
5.4
0.07
7

LAB760
75863.2
97.0
0.07
37
12.1
0.07
37
5.6
0.16
11

LAB736
76368.2
95.3
0.01
35
11.9
0.01
35
5.9
0.01
17

LAB736
76370.1
87.8
0.07
24
11.0
0.07
24
5.4
0.11
7

LAB727
76387.3
95.5
L
35
11.9
L
35
5.9
0.01
16

LAB727
76389.2
83.2
0.01
18
10.4
0.01
18
5.5
0.08
9

LAB698
76201.1
81.8
0.02
16
10.2
0.02
16
5.3
0.18
5

LAB698
76202.2
—
—
—
—
—
—
5.4
0.28
7

LAB698
76204.1
90.6
L
28
11.3
L
28
5.7
L
13

LAB698
76205.1
75.8
0.21
7
9.5
0.21
7
—
—
—

LAB688
75829.1
88.9
0.11
26
11.1
0.11
26
5.5
0.24
9

LAB687
75046.4
76.9
0.18
9
9.6
0.18
9
—
—
—

LAB687
75047.2
83.3
0.17
18
10.4
0.17
18
5.3
0.12
5

LAB677
76528.1
78.3
0.08
11
9.8
0.08
11
5.2
0.26
4

LAB673
76537.1
77.5
0.21
10
—
—
—
—
—
—

LAB650
75838.2
78.2
0.11
11
9.8
0.11
11
5.3
0.25
4

LAB650
75838.3
79.0
0.06
12
9.9
0.06
12
—
—
—

LAB639
76504.3
98.3
L
39
12.3
L
39
6.0
L
18

LAB639
76510.1
77.3
0.13
10
9.7
0.13
10
5.3
0.17
5

LAB625
76452.1
86.3
L
22
10.8
L
22
5.7
0.17
12

LAB625
76452.5
85.3
L
21
10.7
L
21
5.6
0.03
11

LAB625
76472.1
84.9
0.26
20
10.6
0.26
20
5.3
0.22
5

CONT.
—
70.6
—
—
8.8
—
—
5.1
—
—

“CONT.”—Control;

“Ave.”—Average;

“% Incr.” = % increment;

“p-val.”—p-value,

L—p < 0.01.

TABLE 166

Genes showing improved plant performance at drought growth

conditions under regulation of At6669 promoter

RGR Of Leaf

Number
RGR Of Plot
RGR Of Rosette

(Number/day)
Coverage (cm²/day)
Diameter (cm/day)

P-
%

P-
%

P-
%

Gene Name
Event #
Ave.
Val.
Incr.
Ave.
Val.
Incr.
Ave.
Val.
Incr.

LAB795
76491.3
—
—
—
10.2
0.08
41
—
—
—

LAB790
75713.2
—
—
—
9.4
0.19
31
—
—
—

LAB784
76467.4
—
—
—
10.0
0.11
39
—
—
—

LAB769
75309.1
—
—
—
10.2
0.07
42
—
—
—

LAB750
75447.2
—
—
—
9.4
0.18
31
0.49
0.23
19

LAB730
75180.2
0.78
0.18
25
8.9
0.29
24
—
—
—

LAB729
77303.7
—
—
—
9.4
0.18
31
—
—
—

LAB713
75092.1
—
—
—
9.4
0.19
30
—
—
—

LAB670
75303.1
—
—
—
9.0
0.27
25
—
—
—

LAB656
77320.6
0.78
0.22
25
10.9
0.04
51
0.49
0.26
19

LAB637
75105.7
—
—
—
9.0
0.3
25
—
—
—

LAB636
75100.3
—
—
—
10.8
0.04
51
0.50
0.17
22

LAB636
75104.1
—
—
—
9.0
0.3
25
—
—
—

LAB636
75104.8
—
—
—
9.9
0.11
37
0.48
0.3
16

CONT.
—
0.62
—
—
7.2
—
—
0.41
—
—

LAB839
75624.1
—
—
—
14.4
0.04
22
0.53
0.18
8

LAB809
75653.2
—
—
—
14.1
0.08
19
—
—
—

LAB805
76117.2
—
—
—
13.4
0.22
13
0.53
0.25
7

LAB798
75615.4
—
—
—
—
—
—
0.56
0.03
13

LAB798
75616.2
—
—
—
13.4
0.22
13
0.52
0.29
6

LAB792
75876.1
—
—
—
—
—
—
0.56
0.02
14

LAB790
75695.2
—
—
—
—
—
—
0.54
0.15
9

LAB790
75713.2
—
—
—
16.0
L
35
0.59
L
19

LAB740
75265.2
—
—
—
—
—
—
0.54
0.11
9

LAB740
75265.3
—
—
—
13.5
0.17
14
0.53
0.26
6

LAB733
75155.2
—
—
—
—
—
—
0.54
0.14
9

LAB733
75158.5
—
—
—
15.7
L
32
0.53
0.21
7

LAB686
75365.2
0.75
0.12
25
—
—
—
—
—
—

LAB681
75418.2
—
—
—
14.4
0.04
22
—
—
—

LAB681
75421.1
—
—
—
—
—
—
0.54
0.14
8

LAB679
75629.1
—
—
—
13.9
0.14
17
0.55
0.04
11

LAB679
75631.1
0.70
0.27
18
16.2
L
37
—
—
—

LAB665
75040.3
—
—
—
14.6
0.07
23
—
—
—

LAB665
75042.3
—
—
—
13.8
0.14
16
0.54
0.1
10

LAB655
75119.1
—
—
—
14.2
0.07
20
0.54
0.11
9

LAB655
75122.1
—
—
—
14.9
0.02
26
0.55
0.04
11

LAB640
76267.4
—
—
—
—
—
—
0.55
0.06
10

CONT.
—
0.60
—
—
11.8
—
—
0.49
—
—

LAB815
76027.1
0.85
0.14
26
—
—
—
—
—
—

LAB794
75884.1
—
—
—
10.8
0.17
22
—
—
—

LAB748
74972.3
0.83
0.22
22
—
—
—
—
—
—

LAB748
74972.4
0.89
0.08
31
—
—
—
—
—
—

LAB748
74976.2
0.81
0.23
20
—
—
—
—
—
—

LAB732
74966.2
0.80
0.29
19
—
—
—
—
—
—

LAB732
74971.5
—
—
—
10.7
0.19
21
0.49
0.29
11

LAB712
76208.3
0.80
0.27
19
—
—
—
—
—
—

LAB689
74760.1
0.80
0.28
18
10.8
0.16
22
—
—
—

LAB680
74774.3
0.80
0.29
18
11.0
0.12
25
—
—
—

LAB680
74774.4
—
—
—
10.3
0.28
17
—
—
—

LAB666
74840.2
0.81
0.24
20
—
—
—
—
—
—

CONT.
—
0.67
—
—
8.8
—
—
0.44
—
—

LAB837
76256.3
—
—
—
12.1
L
40
0.57
0.03
25

LAB837
76256.5
0.73
0.24
27
10.2
0.07
19
0.52
0.17
15

LAB837
76259.4
0.71
0.28
24
11.5
L
33
0.58
0.02
27

LAB835
75896.5
0.72
0.24
25
9.7
0.22
12
0.54
0.09
19

LAB835
75900.1
—
—
—
—
—
—
0.51
0.26
13

LAB833
75465.1
—
—
—
10.5
0.05
21
0.53
0.17
16

LAB833
75466.1
—
—
—
9.7
0.21
12
0.52
0.18
15

LAB833
75467.3
0.75
0.19
31
—
—
—
—
—
—

LAB800
75331.2
0.79
0.11
37
—
—
—
—
—
—

LAB800
75331.5
—
—
—
10.5
0.05
22
0.51
0.3
12

LAB786
76230.2
—
—
—
9.6
0.24
12
0.52
0.22
14

LAB778
75966.2
—
—
—
9.6
0.28
11
—
—
—

LAB778
75966.3
0.73
0.24
27
11.0
L
27
0.51
0.28
11

LAB778
75970.1
0.73
0.26
27
9.9
0.25
14
—
—
—

LAB776
75749.1
0.71
0.26
24
—
—
—
—
—
—

LAB776
75750.3
—
—
—
—
—
—
0.51
0.25
13

LAB772
75462.2
—
—
—
—
—
—
0.52
0.22
13

LAB768
75455.1
0.80
0.07
38
—
—
—
—
—
—

LAB752
75165.2
—
—
—
—
—
—
0.52
0.17
15

LAB752
75168.1
—
—
—
10.3
0.06
19
—
—
—

LAB752
75168.3
—
—
—
10.6
0.03
23
—
—
—

LAB746
75507.2
0.72
0.24
25
—
—
—
—
—
—

LAB746
75507.8
—
—
—
10.5
0.04
21
0.51
0.27
12

LAB739
75444.5
0.79
0.11
37
9.7
0.22
12
—
—
—

LAB733
75154.3
—
—
—
10.4
0.09
20
—
—
—

LAB733
75158.2
0.71
0.25
24
—
—
—
—
—
—

LAB718
75436.1
0.76
0.12
33
—
—
—
—
—
—

LAB718
75437.2
0.76
0.17
33
—
—
—
—
—
—

LAB718
75439.3
—
—
—
11.0
0.02
28
0.52
0.23
14

LAB716
75431.2
—
—
—
11.0
0.01
27
0.56
0.05
23

LAB681
75419.1
—
—
—
—
—
—
0.53
0.14
16

LAB681
75421.2
0.75
0.16
30
—
—
—
—
—
—

LAB681
75421.4
0.72
0.25
25
—
—
—
—
—
—

CONT.
—
0.58
—
—
8.6
—
—
0.45
—
—

LAB636
75104.1
—
—
—
13.1
0.13
22
—
—
—

LAB632
74960.3
0.87
0.26
16
—
—
—
—
—
—

CONT.
—
0.75
—
—
10.7
—
—
—
—
—

LAB848
77339.2
—
—
—
10.8
0.13
20
—
—
—

LAB842
76350.2
0.80
0.24
22
10.6
0.14
17
—
—
—

LAB842
76350.3
—
—
—
10.3
0.23
14
—
—
—

LAB841
76803.1
—
—
—
11.3
0.04
25
—
—
—

LAB832
77332.4
—
—
—
10.8
0.09
20
—
—
—

LAB829
76442.2
0.81
0.2
23
—
—
—
—
—
—

LAB827
76791.2
—
—
—
10.9
0.08
21
—
—
—

LAB823
77026.4
—
—
—
10.6
0.16
17
—
—
—

LAB823
77027.1
—
—
—
10.2
0.28
13
0.50
0.15
18

LAB803
76760.1
—
—
—
10.5
0.17
16
—
—
—

LAB803
76760.2
—
—
—
10.9
0.08
21
—
—
—

LAB782
76848.6
0.82
0.19
24
—
—
—
—
—
—

LAB770
77765.2
—
—
—
11.2
0.05
23
0.48
0.28
13

LAB767
77705.1
—
—
—
10.6
0.17
17
—
—
—

LAB765
76113.3
—
—
—
10.3
0.25
13
—
—
—

LAB764
76107.5
0.81
0.24
22
10.1
0.3
12
—
—
—

LAB764
76110.5
0.81
0.22
23
10.3
0.27
14
—
—
—

LAB758
76490.4
0.86
0.09
31
12.0
L
32
0.49
0.22
15

LAB757
77326.3
—
—
—
11.5
0.02
27
0.50
0.15
18

LAB757
77326.7
—
—
—
11.0
0.07
21
—
—
—

LAB753
76440.2
—
—
—
10.7
0.13
18
—
—
—

LAB751
76929.2
—
—
—
11.4
0.03
26
0.49
0.22
15

LAB749
77757.4
—
—
—
10.6
0.14
17
—
—
—

LAB742
76432.1
—
—
—
10.5
0.17
16
—
—
—

LAB731
77135.3
—
—
—
11.4
0.08
26
0.50
0.2
19

LAB728
75152.2
0.79
0.29
19
—
—
—
—
—
—

LAB722
76464.1
—
—
—
10.3
0.23
14
—
—
—

LAB722
76465.2
—
—
—
10.9
0.1
20
0.49
0.19
17

LAB717
76430.1
—
—
—
11.8
0.02
31
—
—
—

LAB711
77076.1
—
—
—
11.8
0.01
31
0.49
0.2
16

LAB691
74784.2
—
—
—
10.2
0.25
13
—
—
—

LAB691
74785.4
—
—
—
10.2
0.28
13
0.48
0.25
14

LAB690
77276.2
—
—
—
10.5
0.16
16
0.48
0.27
14

LAB676
77275.1
—
—
—
10.4
0.21
15
—
—
—

LAB672
77699.2
—
—
—
10.3
0.21
14
0.48
0.29
13

LAB672
77700.1
0.80
0.27
20
—
—
—
—
—
—

LAB671
76411.1
0.79
0.28
20
12.1
L
34
0.51
0.11
20

LAB669_H7
77879.4
—
—
—
11.4
0.06
26
—
—
—

LAB663
77046.1
—
—
—
10.4
0.22
14
—
—
—

LAB663
77047.2
—
—
—
10.9
0.08
21
0.50
0.14
18

LAB662
77296.3
—
—
—
10.2
0.27
13
0.48
0.29
13

LAB656
77316.1
—
—
—
11.2
0.04
24
—
—
—

LAB656
77318.2
0.80
0.27
22
10.4
0.18
15
—
—
—

LAB653
75113.1
—
—
—
—
—
—
0.50
0.13
19

LAB641
77221.2
—
—
—
11.2
0.06
23
—
—
—

LAB641
77224.1
0.80
0.28
21
11.0
0.07
21
—
—
—

LAB641
77225.2
—
—
—
13.7
L
51
0.53
0.05
24

LAB633
74831.4
—
—
—
10.3
0.26
14
—
—
—

LAB633
74832.1
—
—
—
10.3
0.25
14
—
—
—

LAB630
77786.1
0.85
0.12
29
—
—
—
—
—
—

LAB629
76896.2
—
—
—
12.0
L
32
0.49
0.22
15

LAB622
75036.2
0.79
0.29
20
—
—
—
—
—
—

LAB622
75036.3
—
—
—
11.6
0.02
28
0.51
0.12
19

CONT.
—
0.66
—
—
9.0
—
—
0.42
—
—

LAB840
75833.2
—
—
—
15.3
0.22
19
—
—
—

LAB839
75624.1
—
—
—
16.1
0.11
25
—
—
—

LAB837
76256.5
0.84
0.22
19
—
—
—
—
—
—

LAB830
76056.1
0.84
0.19
19
—
—
—
—
—
—

LAB830
76060.2
0.84
0.23
18
—
—
—
—
—
—

LAB815
76029.2
—
—
—
15.4
0.19
20
—
—
—

LAB815
76029.4
—
—
—
15.9
0.11
23
—
—
—

LAB804
76281.3
—
—
—
14.8
0.29
15
—
—
—

LAB804
76282.1
—
—
—
16.0
0.11
24
0.56
0.21
16

LAB794
75883.1
0.85
0.18
20
—
—
—
—
—
—

LAB786
76226.1
0.82
0.29
15
—
—
—
—
—
—

LAB777
76274.7
—
—
—
16.8
0.04
31
—
—
—

LAB740
75265.2
0.84
0.25
18
—
—
—
—
—
—

CONT.
—
0.71
—
—
12.9
—
—
0.49
—
—

LAB835
75896.4
—
—
—
8.3
0.19
19
—
—
—

LAB835
75896.5
0.74
0.29
15
—
—
—
—
—
—

LAB835
75900.1
0.74
0.27
15
—
—
—
—
—
—

LAB833
75465.1
—
—
—
8.5
0.14
21
0.48
0.27
10

LAB833
75466.1
—
—
—
—
—
—
0.48
0.25
9

LAB796
75322.1
0.75
0.24
16
8.8
0.08
26
0.49
0.19
11

LAB796
75323.2
—
—
—
8.3
0.19
18
—
—
—

LAB796
75324.1
—
—
—
8.4
0.17
19
0.48
0.27
9

LAB780
75771.2
—
—
—
8.9
0.06
27
0.52
0.06
17

LAB780
75771.3
—
—
—
9.6
0.01
36
0.52
0.06
17

LAB780
75773.3
—
—
—
8.4
0.16
20
—
—
—

LAB778
75967.2
—
—
—
10.1
L
43
0.53
0.02
19

LAB778
75969.2
—
—
—
9.0
0.05
29
0.50
0.13
14

LAB778
75969.4
—
—
—
8.7
0.08
24
—
—
—

LAB776
75746.2
0.76
0.15
18
9.3
0.03
33
0.49
0.22
11

LAB776
75749.1
—
—
—
8.7
0.11
23
0.50
0.15
13

LAB776
75750.3
—
—
—
8.7
0.08
24
0.49
0.2
11

LAB776
75750.4
—
—
—
8.8
0.08
25
0.50
0.11
14

LAB772
75459.1
—
—
—
—
—
—
0.48
0.27
9

LAB768
75453.1
—
—
—
8.3
0.21
18
—
—
—

LAB768
75453.2
—
—
—
8.4
0.16
19
—
—
—

LAB768
75455.1
—
—
—
8.9
0.07
26
0.49
0.21
11

LAB752
75168.1
—
—
—
8.6
0.11
22
0.48
0.27
9

LAB752
75168.3
0.74
0.29
15
—
—
—
—
—
—

LAB739
75441.2
—
—
—
8.4
0.18
19
—
—
—

LAB739
75445.1
—
—
—
8.6
0.1
22
0.48
0.24
10

LAB724
75142.1
—
—
—
9.0
0.04
28
0.51
0.09
14

LAB724
75143.2
—
—
—
8.3
0.2
18
0.48
0.28
9

LAB724
75146.2
—
—
—
8.2
0.25
16
0.49
0.22
11

LAB718
75436.1
—
—
—
8.4
0.17
20
0.50
0.12
14

LAB716
75429.3
—
—
—
8.3
0.2
17
0.48
0.28
9

LAB716
75430.5
—
—
—
8.7
0.1
24
0.54
0.02
22

LAB705
74879.2
—
—
—
9.5
0.02
35
0.53
0.02
20

LAB705
74879.3
—
—
—
8.7
0.11
23
0.49
0.18
11

LAB705
74881.1
—
—
—
8.4
0.17
19
—
—
—

LAB686
75364.4
—
—
—
9.1
0.04
30
0.51
0.09
15

CONT.
—
0.64
—
—
7.0
—
—
0.44
—
—

LAB670
75306.3
—
—
—
10.6
0.24
31
—
—
—

CONT.
—
—
—
—
8.1
—
—
—
—
—

LAB834
75895.3
—
—
—
13.4
0.05
30
0.59
0.16
17

LAB793
75751.3
—
—
—
13.4
0.12
31
0.60
0.22
19

LAB793
75755.3
0.45
0.29
38
—
—
—
—
—
—

LAB787
76754.1
0.46
0.27
42
—
—
—
—
—
—

LAB760
75863.1
0.56
0.07
74
—
—
—
—
—
—

LAB760
75863.2
—
—
—
14.0
0.03
36
—
—
—

LAB736
76368.2
—
—
—
13.6
0.04
32
—
—
—

LAB736
76370.1
—
—
—
12.8
0.12
24
—
—
—

LAB727
76387.3
—
—
—
13.8
0.03
34
0.58
0.22
15

LAB727
76389.2
—
—
—
12.0
0.26
17
—
—
—

LAB726
76382.1
0.46
0.28
41
—
—
—
—
—
—

LAB698
76204.1
—
—
—
12.9
0.1
25
—
—
—

LAB688
75829.1
—
—
—
13.2
0.08
28
—
—
—

LAB687
75047.2
—
—
—
12.1
0.25
17
—
—
—

LAB650
75838.4
—
—
—
12.5
0.24
21
—
—
—

LAB639
76504.3
—
—
—
14.3
0.01
39
0.59
0.16
17

LAB625
76452.1
—
—
—
12.7
0.13
23
—
—
—

LAB625
76452.5
—
—
—
12.5
0.16
21
0.58
0.25
14

LAB625
76472.1
—
—
—
12.0
0.29
17
—
—
—

CONT.
—
0.32
—
—
10.3
—
—
0.51
—
—

“CONT.”—Control;

“Ave.”—Average;

“% Incr.” = % increment;

“p-val.”—p-value,

L—p < 0.01.

TABLE 167

Genes showing improved plant performance at normal growth conditions under

regulation of At6669 promoter

Dry Weight [mg]
Fresh Weight [mg,]
Leaf Number

Gene

P-
%

P-
%

P-
%

Name
Event #
Ave.
Val.
Incr.
Ave.
Val.
Incr.
Ave.
Val.
Incr.

LAB824
75502.2
175.0
L
21
1975.0
0.15
15
—
—
—

LAB824
75504.2
169.2
0.02
17
1929.5
0.13
13
—
—
—

LAB824
75505.3
189.4
0.09
31
—
—
—
—
—
—

LAB795
76491.3
161.5
0.18
12
2033.0
0.11
19
—
—
—

LAB795
76494.6
—
—
—
1946.4
0.20
14
—
—
—

LAB792
75876.3
162.5
0.09
13
1887.5
0.24
10
—
—
—

LAB792
75878.3
161.9
0.12
12
—
—
—
9.9
0.06
4

LAB792
75880.4
—
—
—
2150.0
0.26
25
10.9
0.05
14

LAB790
75695.1
—
—
—
1862.5
0.28
9
—
—
—

LAB790
75713.2
154.4
0.23
7
—
—
—
—
—
—

LAB789
77081.4
159.8
0.22
11
—
—
—
—
—
—

LAB789
77085.3
160.0
0.13
11
1968.8
0.15
15
—
—
—

LAB784
76467.4
176.9
0.02
23
2012.5
0.09
17
9.8
0.29
2

LAB784
76468.3
180.0
0.15
25
2118.8
0.13
24
9.8
0.17
2

LAB784
76470.2
168.8
0.21
17
1881.2
0.27
10
—
—
—

LAB769
75309.1
166.9
0.06
16
1925.0
0.11
12
9.9
0.13
4

LAB769
75311.5
—
—
—
—
—
—
9.8
0.11
3

LAB769
75348.4
183.8
0.20
27
2056.2
0.28
20
10.2
L
7

LAB750
75448.4
—
—
—
—
—
—
9.7
0.29
2

LAB730
75180.3
—
—
—
—
—
—
10.0
0.06
5

LAB729
77303.7
—
—
—
—
—
—
9.8
0.17
2

LAB649
77041.3
164.6
0.04
14
1915.2
0.12
12
—
—
—

LAB649
77041.4
175.6
L
22
2000.0
0.04
17
—
—
—

LAB649
77045.3
183.1
0.24
27
—
—
—
10.1
0.12
6

LAB637
75105.8
185.0
0.17
28
2143.8
0.17
25
10.4
0.16
10

LAB636
75100.3
180.0
0.06
25
2093.8
0.20
22
—
—
—

LAB636
75104.8
163.8
0.28
14
—
—
—
10.1
0.12
6

LAB632
74960.2
172.5
0.01
20
1918.8
0.11
12
—
—
—

CONT.
—
144.2
—
—
1713.6
—
—
9.5
—
—

LAB839
75623.2
—
—
—
—
—
—
10.9
0.25
6

LAB839
75623.3
—
—
—
—
—
—
11.5
0.03
12

LAB839
75624.1
—
—
—
881.2
0.24
19
12.4
0.13
20

LAB831
76254.1
—
—
—
—
—
—
11.0
0.12
7

LAB809
75651.2
—
—
—
—
—
—
11.0
0.12
7

LAB809
75651.3
—
—
—
—
—
—
10.9
0.17
5

LAB792
75876.1
—
—
—
—
—
—
10.8
0.21
5

LAB783
75980.5
—
—
—
—
—
—
10.8
0.27
4

LAB783
75980.6
—
—
—
—
—
—
11.2
0.06
9

LAB762
75866.2
—
—
—
900.0
0.17
22
11.2
0.24
8

LAB762
75870.2
—
—
—
—
—
—
10.8
0.25
5

LAB740
75264.1
—
—
—
—
—
—
11.4
0.06
10

LAB740
75265.3
—
—
—
—
—
—
11.0
0.27
7

LAB733
75158.5
—
—
—
—
—
—
11.1
0.12
8

LAB686
75364.6
—
—
—
903.6
0.17
23
12.2
0.20
19

LAB686
75365.2
—
—
—
—
—
—
10.9
0.24
5

LAB681
75418.2
—
—
—
—
—
—
11.4
0.04
11

LAB679
75631.1
—
—
—
—
—
—
11.4
0.14
10

LAB665
75040.3
88.8
0.17
18
881.2
0.28
19
12.2
0.04
19

LAB655
75119.2
—
—
—
—
—
—
11.2
0.14
8

LAB655
75122.1
—
—
—
—
—
—
10.9
0.17
5

CONT.
—
75.4
—
—
737.5
—
—
10.3
—
—

LAB815
76027.2
—
—
—
1558.3
0.28
7
—
—
—

LAB815
76030.1
—
—
—
—
—
—
10.6
0.08
4

LAB794
75881.2
—
—
—
—
—
—
10.9
0.08
7

LAB794
75883.1
—
—
—
1512.5
0.16
4
—
—
—

LAB794
75883.2
—
—
—
—
—
—
11.1
0.28
8

LAB774
76372.2
145.0
0.08
23
1606.2
L
10
—
—
—

LAB774
76374.1
127.5
0.16
8
—
—
—
—
—
—

LAB774
76374.2
—
—
—
1601.8
0.02
10
—
—
—

LAB774
76375.3
—
—
—
—
—
—
10.6
0.25
4

LAB762
75866.5
128.1
0.12
9
1531.2
0.08
5
—
—
—

LAB762
75869.2
126.9
0.21
8
1512.5
0.16
4
10.6
0.08
4

LAB762
75869.3
—
—
—
—
—
—
10.9
L
7

LAB748
74972.3
—
—
—
—
—
—
11.1
0.01
9

LAB748
74972.4
—
—
—
1540.2
0.06
6
10.8
0.11
6

LAB748
74976.2
—
—
—
—
—
—
10.5
0.17
3

LAB732
74966.2
133.1
0.25
13
—
—
—
—
—
—

LAB732
74969.1
138.8
0.05
18
—
—
—
—
—
—

LAB732
74971.3
142.8
0.13
21
1661.6
0.17
14
—
—
—

LAB712
76210.1
133.1
0.19
13
1584.8
0.04
9
—
—
—

LAB706
74799.1
—
—
—
—
—
—
10.5
0.04
3

LAB695
74793.1
—
—
—
1507.1
0.20
4
—
—
—

LAB695
74797.4
137.4
0.20
17
1558.9
0.03
7
—
—
—

LAB689
74756.1
127.1
0.19
8
—
—
—
—
—
—

LAB689
74756.2
—
—
—
1567.9
0.03
8
10.8
0.23
5

LAB689
74756.3
125.4
0.24
7
—
—
—
10.8
0.05
5

LAB689
74758.4
126.9
0.26
8
1625.0
L
12
11.0
0.02
8

LAB689
74760.1
—
—
—
—
—
—
10.8
L
6

LAB680
74774.3
150.0
L
28
1620.8
L
11
10.4
0.18
2

LAB680
74774.4
131.9
0.07
12
—
—
—
10.4
0.12
2

LAB680
74775.1
—
—
—
—
—
—
10.7
0.16
5

LAB680
74777.1
139.7
0.07
19
—
—
—
—
—
—

LAB666
74840.5
—
—
—
1525.0
0.17
5
—
—
—

LAB666
74841.2
137.5
0.06
17
—
—
—
—
—
—

LAB666
74844.3
125.8
0.21
7
—
—
—
—
—
—

LAB666
74845.1
135.4
0.27
15
1637.5
0.03
13
—
—
—

LAB624
77106.1
—
—
—
1600.0
0.06
10
—
—
—

LAB624
77110.4
141.9
0.28
21
—
—
—
—
—
—

LAB619
77311.1
138.1
0.12
17
1556.2
0.10
7
—
—
—

LAB619
77311.3
—
—
—
1562.5
0.03
7
—
—
—

LAB619
77312.2
126.9
0.18
8
—
—
—
—
—
—

LAB619
77314.2
128.1
0.12
9
—
—
—
—
—
—

CONT.
—
117.6
—
—
1454.6
—
—
10.2
—
—

LAB837
76256.2
—
—
—
2021.4
0.22
14
—
—
—

LAB837
76256.3
—
—
—
2069.6
0.23
17
—
—
—

LAB837
76259.4
—
—
—
1922.3
0.13
9
9.4
0.24
3

LAB833
75468.4
—
—
—
2018.7
0.03
14
—
—
—

LAB800
75331.2
—
—
—
—
—
—
9.4
0.24
3

LAB800
75331.5
—
—
—
1943.8
0.08
10
—
—
—

LAB786
76229.1
—
—
—
1887.5
0.21
7
—
—
—

LAB786
76230.2
—
—
—
—
—
—
9.9
0.15
7

LAB786
76230.7
—
—
—
2075.0
0.18
17
9.5
0.22
3

LAB780
75771.2
—
—
—
2059.8
0.01
17
—
—
—

LAB780
75773.3
—
—
—
1894.6
0.18
7
—
—
—

LAB778
75966.2
192.9
0.21
12
—
—
—
—
—
—

LAB778
75966.3
—
—
—
—
—
—
9.5
0.22
3

LAB778
75969.4
—
—
—
1907.1
0.14
8
—
—
—

LAB776
75750.3
—
—
—
1963.4
0.09
11
—
—
—

LAB772
75461.6
—
—
—
2000.0
0.18
13
—
—
—

LAB772
75462.5
—
—
—
2035.7
0.24
15
—
—
—

LAB768
75453.1
—
—
—
1890.2
0.26
7
9.9
0.15
7

LAB768
75453.3
—
—
—
—
—
—
9.6
0.09
4

LAB752
75165.2
—
—
—
1887.5
0.23
7
—
—
—

LAB746
75508.3
—
—
—
—
—
—
9.6
0.10
5

LAB739
75442.1
—
—
—
1967.9
0.05
11
—
—
—

LAB739
75444.5
—
—
—
2012.5
0.06
14
—
—
—

LAB733
75154.3
—
—
—
—
—
—
9.4
0.24
3

LAB733
75155.2
—
—
—
—
—
—
9.8
0.09
7

LAB733
75158.3
—
—
—
1950.0
0.07
10
—
—
—

LAB718
75437.2
—
—
—
—
—
—
9.7
0.04
5

LAB716
75429.3
—
—
—
1943.8
0.21
10
9.9
L
7

LAB686
75363.1
205.5
0.06
20
1918.8
0.12
9
—
—
—

LAB681
75419.1
—
—
—
1875.0
0.24
6
—
—
—

LAB681
75421.4
—
—
—
—
—
—
9.7
0.04
5

CONT.
—
171.9
—
—
1767.6
—
—
9.2
—
—

LAB824
75502.2
165.0
0.07
10
—
—
—
—
—
—

LAB824
75504.1
161.7
0.17
8
—
—
—
—
—
—

LAB795
76500.3
176.2
0.05
18
2012.5
0.13
16
—
—
—

LAB792
75876.3
177.9
0.09
19
1956.2
0.03
13
—
—
—

LAB789
77084.4
—
—
—
—
—
—
11.2
0.24
5

LAB789
77085.3
167.8
0.04
12
1966.1
0.18
14
—
—
—

LAB784
76469.1
164.4
0.10
10
—
—
—
11.4
0.08
6

LAB769
75345.1
166.1
0.27
11
—
—
—
—
—
—

LAB730
75182.1
161.2
0.11
8
1831.2
0.14
6
—
—
—

LAB729
77303.7
—
—
—
1906.2
0.01
10
—
—
—

LAB708
75288.5
175.4
L
17
1930.4
0.08
12
—
—
—

LAB670
75306.3
—
—
—
—
—
—
11.0
0.27
2

LAB649
77041.4
161.9
0.16
8
1806.2
0.19
4
—
—
—

LAB649
77042.1
162.5
0.08
8
1825.0
0.13
5
—
—
—

LAB649
77044.2
158.1
0.25
5
—
—
—
—
—
—

LAB649
77045.3
—
—
—
1881.2
0.02
9
—
—
—

LAB637
75105.6
—
—
—
—
—
—
11.2
0.24
5

LAB637
75105.8
183.8
0.01
22
2031.2
0.01
17
11.6
0.18
8

LAB637
75105.9
173.2
0.26
15
—
—
—
—
—
—

LAB636
75100.3
176.9
0.11
18
—
—
—
—
—
—

LAB636
75104.1
158.8
0.20
6
1893.8
0.02
9
11.5
0.13
7

LAB632
74960.3
161.9
0.22
8
—
—
—
—
—
—

LAB618
77782.1
162.5
0.07
8
1793.8
0.25
4
—
—
—

CONT.
—
150.0
—
—
1731.0
—
—
10.8
—
—

LAB840
75832.6
195.9
0.27
9
—
—
—
—
—
—

LAB840
75833.1
—
—
—
—
—
—
11.5
0.15
3

LAB840
75833.2
—
—
—
—
—
—
11.8
0.08
6

LAB839
75623.4
197.2
0.25
10
—
—
—
—
—
—

LAB837
76256.2
188.8
0.25
5
—
—
—
—
—
—

LAB830
76060.2
—
—
—
—
—
—
11.6
0.06
4

LAB830
76060.4
189.1
0.12
6
—
—
—
—
—
—

LAB815
76027.2
—
—
—
—
—
—
11.4
0.22
2

LAB804
76281.1
—
—
—
—
—
—
11.4
0.15
3

LAB804
76284.1
—
—
—
1906.2
0.26
7
—
—
—

LAB801
75886.5
—
—
—
—
—
—
11.4
0.15
3

LAB801
75887.2
—
—
—
1925.0
0.29
8
—
—
—

LAB801
75889.1
186.0
0.06
4
—
—
—
—
—
—

LAB800
75355.3
—
—
—
1993.8
0.01
12
—
—
—

LAB794
75881.2
—
—
—
—
—
—
11.5
0.15
3

LAB794
75883.1
199.4
0.21
11
—
—
—
—
—
—

LAB794
75884.2
—
—
—
—
—
—
11.8
0.18
5

LAB788
76232.2
—
—
—
—
—
—
11.5
0.15
3

LAB788
76234.1
226.9
0.27
27
2143.8
0.03
21
—
—
—

LAB786
76229.1
—
—
—
—
—
—
11.8
0.03
5

LAB786
76230.2
186.8
0.06
4
—
—
—
—
—
—

LAB786
76230.6
190.0
0.13
6
—
—
—
—
—
—

LAB777
76273.3
190.0
0.04
6
—
—
—
—
—
—

LAB777
76274.7
195.0
0.30
9
—
—
—
—
—
—

LAB704
75132.1
194.4
L
9
1968.8
0.23
11
—
—
—

LAB642
75912.1
—
—
—
2062.5
0.11
16
—
—
—

LAB642
75914.1
185.6
0.07
4
—
—
—
—
—
—

LAB642
75936.2
—
—
—
1868.8
0.23
5
11.6
0.20
4

CONT.
—
179.1
—
—
1776.8
—
—
11.1
—
—

LAB835
75896.3
147.5
0.26
19
1655.4
0.14
33
10.6
L
11

LAB835
75896.4
153.1
0.21
23
1562.5
0.01
26
10.2
0.02
8

LAB835
75900.1
171.2
0.24
38
1512.5
0.01
22
10.1
0.14
6

LAB833
75466.1
148.8
0.17
20
1600.0
0.10
29
9.8
0.24
3

LAB833
75467.3
144.7
0.19
16
—
—
—
9.8
0.05
3

LAB796
75321.2
—
—
—
—
—
—
9.7
0.19
2

LAB796
75322.1
160.0
0.03
29
1631.2
0.15
31
10.6
0.05
11

LAB796
75323.2
—
—
—
1431.2
0.24
15
—
—
—

LAB796
75325.2
—
—
—
1325.0
0.26
7
10.2
0.24
7

LAB780
75771.2
—
—
—
1418.8
0.26
14
10.6
0.15
11

LAB780
75771.3
136.9
0.20
10
1425.0
0.22
15
10.2
0.02
8

LAB780
75773.3
146.9
0.29
18
1481.2
0.08
19
10.4
0.13
9

LAB778
75967.2
142.7
0.05
15
1650.0
L
33
11.1
0.18
17

LAB778
75969.2
—
—
—
1406.2
0.09
13
—
—
—

LAB778
75969.4
—
—
—
—
—
—
10.3
0.08
9

LAB776
75746.2
139.8
0.10
13
1509.8
0.03
21
10.6
0.22
11

LAB776
75749.1
158.8
0.18
28
1556.2
0.02
25
9.9
0.01
5

LAB776
75750.3
166.2
L
34
1762.5
L
42
10.8
L
14

LAB776
75750.4
154.4
0.01
24
1581.2
0.06
27
10.6
0.09
12

LAB772
75459.1
—
—
—
1587.5
L
28
10.2
L
8

LAB772
75461.6
148.8
0.06
20
1531.2
L
23
10.5
L
11

LAB772
75462.5
—
—
—
1325.9
0.26
7
—
—
—

LAB768
75453.1
159.4
0.03
28
1706.2
L
37
10.4
0.06
10

LAB768
75453.2
142.3
0.09
15
—
—
—
10.4
0.24
9

LAB768
75453.3
166.2
0.11
34
1743.8
0.13
40
10.2
L
7

LAB768
75455.1
141.8
0.07
14
1492.9
L
20
10.6
0.15
11

LAB768
75456.1
—
—
—
—
—
—
9.7
0.19
2

LAB752
75168.1
141.2
0.09
14
1512.5
L
22
10.5
0.28
11

LAB746
75507.2
—
—
—
1406.2
0.05
13
10.2
0.02
8

LAB746
75508.2
—
—
—
—
—
—
9.8
0.07
3

LAB746
75508.3
—
—
—
1339.3
0.19
8
—
—
—

LAB739
75442.1
—
—
—
—
—
—
10.2
0.10
7

LAB739
75444.4
148.8
0.13
20
1525.0
L
23
10.3
0.30
9

LAB739
75445.1
—
—
—
1512.5
L
22
10.5
L
11

LAB733
75154.3
—
—
—
—
—
—
10.5
L
11

LAB733
75157.2
—
—
—
—
—
—
9.8
0.24
3

LAB733
75158.2
147.5
0.02
19
1475.0
0.01
19
10.1
L
7

LAB724
75142.1
170.6
0.20
37
1531.2
0.01
23
—
—
—

LAB724
75143.2
141.9
0.06
14
1537.5
L
24
—
—
—

LAB724
75144.4
—
—
—
—
—
—
10.1
0.30
6

LAB724
75146.2
141.9
0.07
14
1600.0
L
29
10.5
L
11

LAB718
75435.2
—
—
—
—
—
—
10.0
0.06
5

LAB718
75437.2
163.1
L
31
1593.8
0.18
28
10.1
0.14
6

LAB718
75439.2
151.9
0.23
22
1481.2
0.01
19
—
—
—

LAB718
75439.3
—
—
—
—
—
—
10.0
L
5

LAB716
75429.3
155.5
L
25
1497.3
0.02
20
10.1
L
6

LAB716
75430.5
171.0
L
38
1665.2
L
34
10.2
0.25
7

LAB716
75431.2
159.8
0.03
29
1507.1
0.14
21
10.8
0.08
13

LAB716
75431.4
—
—
—
1395.5
0.19
12
—
—
—

LAB705
74876.2
—
—
—
—
—
—
10.0
0.06
5

LAB705
74879.2
158.1
0.28
27
1600.0
L
29
—
—
—

LAB705
74879.3
—
—
—
—
—
—
10.1
L
7

LAB705
74881.1
143.1
0.05
15
—
—
—
—
—
—

LAB705
74881.2
—
—
—
1528.6
0.14
23
—
—
—

LAB686
75364.1
—
—
—
1500.0
0.13
21
—
—
—

LAB686
75364.4
—
—
—
—
—
—
10.6
0.23
11

LAB681
75419.1
—
—
—
—
—
—
9.9
0.01
5

LAB681
75420.4
—
—
—
1368.8
0.11
10
10.4
L
10

LAB681
75421.4
—
—
—
1433.0
0.27
15
10.8
0.08
13

CONT.
—
124.3
—
—
1243.6
—
—
9.5
—
—

LAB800
75331.6
248.8
0.11
47
2937.5
0.16
37
—
—
—

LAB800
75355.3
208.1
0.26
23
—
—
—
—
—
—

LAB791
75316.2
281.2
0.03
66
2759.8
0.23
29
—
—
—

LAB791
75317.2
245.6
0.07
45
—
—
—
—
—
—

LAB791
75318.2
308.5
0.21
82
3308.0
0.07
55
—
—
—

LAB791
75319.5
226.2
0.24
33
—
—
—
—
—
—

LAB755
75172.7
225.4
0.18
33
—
—
—
—
—
—

LAB730
75177.3
209.4
0.23
23
—
—
—
—
—
—

LAB713
75090.2
261.2
0.20
54
—
—
—
—
—
—

LAB708
75342.1
223.1
0.23
31
—
—
—
—
—
—

LAB704
75132.3
219.4
0.16
29
2825.0
0.19
32
—
—
—

LAB704
75132.4
230.5
0.13
36
—
—
—
—
—
—

LAB704
75133.1
264.4
0.05
56
2787.5
0.21
30
—
—
—

LAB637
75105.1
205.9
0.26
21
2683.9
0.27
25
—
—
—

CONT.
—
169.7
—
—
2138.9
—
—
—
—
—

LAB834
75892.5
171.9
0.11
39
1606.2
0.09
24
10.4
0.19
4

LAB834
75895.5
150.7
0.12
22
—
—
—
—
—
—

LAB807
76240.1
153.8
0.27
24
—
—
—
10.6
0.02
6

LAB807
76240.4
—
—
—
1462.5
0.28
13
—
—
—

LAB793
75751.2
175.9
0.21
42
1743.8
L
34
10.7
0.21
7

LAB793
75755.1
—
—
—
1718.8
L
32
—
—
—

LAB736
76368.2
—
—
—
1462.5
0.18
13
—
—
—

LAB736
76370.1
—
—
—
—
—
—
10.4
0.19
4

LAB736
76370.3
168.9
L
37
1505.4
0.17
16
—
—
—

LAB736
76370.4
169.6
0.29
37
—
—
—
—
—
—

LAB727
76387.3
154.6
0.16
25
1735.4
0.30
34
10.5
0.04
5

LAB726
76385.3
—
—
—
1512.5
0.08
17
—
—
—

LAB707
75855.4
161.2
L
30
—
—
—
—
—
—

LAB698
76204.1
163.8
0.18
32
1560.7
0.18
20
—
—
—

LAB688
75829.1
145.6
0.06
18
1725.0
0.01
33
10.3
0.17
3

LAB687
75046.4
148.8
0.13
20
1675.0
0.22
29
—
—
—

LAB687
75047.2
—
—
—
—
—
—
11.4
L
14

LAB677
76524.1
—
—
—
—
—
—
10.4
0.19
4

LAB677
76528.1
—
—
—
1583.0
0.24
22
—
—
—

LAB673
76532.1
—
—
—
—
—
—
10.5
0.23
5

LAB673
76533.1
137.9
0.21
11
1635.0
0.01
26
10.5
0.07
5

LAB673
76537.1
—
—
—
—
—
—
10.6
0.11
6

LAB650
75838.3
—
—
—
—
—
—
10.9
L
9

LAB639
76504.3
—
—
—
1679.5
0.08
29
10.3
0.17
3

LAB625
76452.1
176.4
0.27
43
1614.3
0.07
24
—
—
—

LAB625
76453.1
—
—
—
—
—
—
10.3
0.29
3

LAB625
76473.1
—
—
—
1565.2
0.09
21
—
—
—

LAB616
76732.4
163.2
0.03
32
1479.5
0.25
14
—
—
—

CONT.
—
123.8
—
—
1298.2
—
—
10.0
—
—

Table 167. “CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment; “p-val.”—p-value, L—p < 0.01.

TABLE 168

Genes showing improved plant performance at normal growth conditions under

regulation of At6669 promoter

Plot Coverage [cm²]
Rosette Area [cm²]
Rosette Diameter [cm]

Gene

P-
%

P-
%

P-
%

Name
Event #
Ave.
Val.
Incr.
Ave.
Val.
Incr.
Ave.
Val.
Incr.

LAB824
75502.2
68.8
0.19
17
8.6
0.19
17
5.0
0.16
8

LAB795
76491.3
71.1
0.20
21
8.9
0.20
21
—
—
—

LAB795
76494.3
73.0
0.30
24
9.1
0.30
24
5.1
0.26
10

LAB795
76494.6
70.0
0.24
19
8.7
0.24
19
—
—
—

LAB792
75876.3
70.7
0.19
20
8.8
0.19
20
—
—
—

LAB792
75878.3
74.5
0.02
27
9.3
0.02
27
5.2
0.03
13

LAB792
75880.4
73.7
0.13
25
9.2
0.13
25
5.1
0.09
10

LAB789
77085.3
71.4
0.16
21
8.9
0.16
21
5.1
0.08
11

LAB784
76467.4
70.3
0.06
20
8.8
0.06
20
5.0
0.10
9

LAB784
76468.3
73.1
0.16
24
9.1
0.16
24
5.1
0.05
12

LAB784
76470.2
73.9
0.02
26
9.2
0.02
26
5.0
0.07
10

LAB769
75309.1
72.4
0.04
23
9.1
0.04
23
5.1
0.06
11

LAB769
75348.4
75.5
0.02
28
9.4
0.02
28
5.1
0.04
12

LAB649
77041.4
67.9
0.18
16
8.5
0.18
16
5.0
0.24
8

LAB649
77045.3
77.6
0.28
32
9.7
0.28
32
—
—
—

LAB637
75105.8
82.3
0.12
40
10.3
0.12
40
5.4
0.14
17

LAB636
75100.3
76.9
0.04
31
9.6
0.04
31
5.2
0.21
13

LAB636
75104.8
66.0
0.22
12
8.2
0.22
12
—
—
—

CONT.
—
58.8
—
—
7.3
—
—
4.6
—
—

LAB839
75624.1
145.5
0.09
33
18.2
0.09
33
6.9
0.09
13

LAB783
75980.6
—
—
—
—
—
—
6.6
0.16
8

LAB762
75866.2
134.2
0.16
23
16.8
0.16
23
6.7
0.10
9

LAB686
75364.6
134.0
0.22
23
16.7
0.22
23
—
—
—

LAB679
75631.1
127.8
0.21
17
16.0
0.21
17
6.4
0.24
6

LAB665
75040.3
132.2
0.25
21
16.5
0.25
21
—
—
—

LAB665
75042.3
139.8
0.08
28
17.5
0.08
28
6.8
0.06
11

CONT.
—
109.0
—
—
13.6
—
—
6.1
—
—

LAB815
76026.1
83.9
0.02
13
10.5
0.02
13
—
—
—

LAB815
76027.2
79.5
0.14
8
9.9
0.14
8
—
—
—

LAB815
76030.1
87.6
0.11
19
11.0
0.11
19
5.5
0.19
9

LAB794
75881.2
85.7
0.30
16
10.7
0.30
16
5.4
0.28
8

LAB794
75883.2
81.6
0.04
10
10.2
0.04
10
—
—
—

LAB794
75884.1
82.9
0.26
12
10.4
0.26
12
5.3
0.27
6

LAB774
76372.2
—
—
—
—
—
—
5.3
0.26
5

LAB762
75866.5
80.5
0.20
9
10.1
0.20
9
5.5
0.01
8

LAB762
75869.2
81.0
0.06
10
10.1
0.06
10
5.3
0.19
6

LAB762
75870.3
78.7
0.17
6
9.8
0.17
6
5.2
0.26
3

LAB748
74972.1
77.6
0.27
5
9.7
0.27
5
—
—
—

LAB748
74972.3
81.9
0.04
11
10.2
0.04
11
5.3
0.08
5

LAB748
74972.4
83.3
0.06
13
10.4
0.06
13
5.3
0.19
6

LAB748
74976.2
86.5
0.12
17
10.8
0.12
17
—
—
—

LAB732
74966.2
—
—
—
—
—
—
5.4
0.03
6

LAB732
74971.5
84.9
0.01
15
10.6
0.01
15
5.3
0.10
4

LAB706
74803.4
78.8
0.17
7
9.8
0.17
7
—
—
—

LAB695
74797.4
84.8
0.21
15
10.6
0.21
15
—
—
—

LAB689
74756.2
—
—
—
—
—
—
5.2
0.14
4

LAB689
74758.4
96.3
0.01
30
12.0
0.01
30
5.7
L
13

LAB689
74760.1
93.4
0.05
26
11.7
0.05
26
5.8
L
14

LAB683
74871.3
82.7
0.27
12
10.3
0.27
12
—
—
—

LAB683
74873.1
84.4
0.22
14
10.5
0.22
14
5.4
0.13
6

LAB680
74774.3
—
—
—
—
—
—
5.2
0.23
3

LAB680
74774.4
79.7
0.10
8
10.0
0.10
8
5.3
0.11
6

LAB680
74777.1
—
—
—
—
—
—
5.3
0.20
5

LAB666
74845.1
—
—
—
—
—
—
5.2
0.16
4

LAB619
77311.3
84.3
0.13
14
10.5
0.13
14
5.3
0.11
6

LAB619
77314.3
80.0
0.15
8
10.0
0.15
8
—
—
—

CONT.
—
73.9
—
—
9.2
—
—
5.0
—
—

LAB837
76256.3
62.8
0.02
10
7.8
0.02
10
4.9
0.02
5

LAB837
76259.4
67.9
0.13
19
8.5
0.13
19
5.2
0.26
10

LAB833
75468.4
—
—
—
—
—
—
4.8
0.29
2

LAB800
75331.5
65.0
0.01
14
8.1
0.01
14
5.0
L
7

LAB786
76229.1
60.5
0.12
6
7.6
0.12
6
4.9
0.21
4

LAB786
76230.2
68.1
L
19
8.5
L
19
5.2
0.08
11

LAB780
75771.2
—
—
—
—
—
—
4.9
0.02
5

LAB780
75773.3
60.1
0.15
5
7.5
0.15
5
—
—
—

LAB776
75750.3
—
—
—
—
—
—
4.9
0.11
5

LAB772
75461.6
—
—
—
8.4
L
17
5.0
0.10
7

LAB768
75453.1
62.0
0.12
8
7.7
0.12
8
—
—
—

LAB752
75165.2
64.3
L
12
8.0
L
12
5.0
L
7

LAB752
75168.1
62.0
0.03
8
7.7
0.03
8
4.8
0.06
3

LAB746
75507.8
—
—
—
—
—
—
4.9
0.19
4

LAB746
75508.3
64.2
0.03
12
8.0
0.03
12
5.0
0.15
7

LAB739
75442.1
60.2
0.30
5
7.5
0.30
5
—
—
—

LAB739
75444.5
—
—
—
—
—
—
5.1
0.19
10

LAB718
75437.2
62.9
0.08
10
7.9
0.08
10
5.0
0.11
7

LAB716
75429.3
—
—
—
—
—
—
5.1
0.13
10

LAB716
75431.2
66.6
0.03
16
8.3
0.03
16
5.0
0.04
7

LAB681
75419.1
64.1
0.13
12
8.0
0.13
12
5.0
L
6

CONT.
—
57.2
—
—
7.2
—
—
4.7
—
—

LAB792
75876.3
98.1
0.25
18
12.3
0.25
18
—
—
—

LAB789
77085.3
—
—
—
11.8
0.10
14
—
—
—

LAB730
75182.1
95.9
0.03
15
12.0
0.03
15
—
—
—

LAB649
77042.1
91.3
0.11
10
11.4
0.11
10
—
—
—

LAB649
77045.3
94.8
0.04
14
11.9
0.04
14
6.0
0.03
9

LAB637
75105.8
97.5
0.13
17
12.2
0.13
17
5.7
0.24
4

LAB636
75100.3
92.9
0.07
12
11.6
0.07
12
5.8
0.14
5

LAB636
75104.1
110.2
L
33
13.8
L
33
6.2
0.08
13

LAB618
77785.2
—
—
—
—
—
—
5.9
0.19
7

CONT.
—
83.0
—
—
10.4
—
—
5.5
—
—

LAB840
75833.2
—
—
—
—
—
—
6.0
0.25
4

LAB801
75887.2
114.4
0.29
15
14.3
0.29
15
6.4
0.09
11

LAB800
75355.3
108.5
0.20
9
13.6
0.20
9
6.0
0.28
4

LAB788
76234.1
—
—
—
—
—
—
6.3
0.23
10

LAB786
76230.6
—
—
—
—
—
—
6.0
0.26
4

LAB777
76274.7
114.6
0.09
15
14.3
0.09
15
6.1
0.11
6

LAB704
75132.1
106.8
0.19
7
13.4
0.19
7
6.1
0.14
5

LAB704
75132.2
112.8
0.04
13
14.1
0.04
13
6.3
0.02
9

LAB642
75912.1
108.0
0.14
8
13.5
0.14
8
6.2
0.05
7

CONT.
—
99.7
—
—
12.5
—
—
5.8
—
—

LAB835
75896.3
62.7
0.06
30
7.8
0.06
30
4.8
L
11

LAB835
75896.4
59.2
0.19
23
7.4
0.19
23
4.8
0.28
9

LAB835
75900.1
59.7
0.21
24
7.5
0.21
24
4.8
0.11
11

LAB833
75466.1
63.1
0.05
31
7.9
0.05
31
5.2
L
21

LAB833
75467.3
52.6
0.22
9
6.6
0.22
9
—
—
—

LAB833
75468.4
54.4
0.01
13
6.8
0.01
13
4.5
0.29
3

LAB796
75322.1
66.0
0.20
37
8.2
0.20
37
5.1
0.17
17

LAB780
75771.2
56.6
0.26
18
7.1
0.26
18
4.7
0.05
8

LAB780
75771.3
58.7
L
22
7.3
L
22
4.7
0.01
8

LAB780
75773.3
60.5
0.12
26
7.6
0.12
26
4.7
0.02
9

LAB778
75967.2
65.4
L
36
8.2
L
36
4.9
L
13

LAB778
75969.2
56.6
L
18
7.1
L
18
4.7
0.04
9

LAB776
75746.2
60.7
0.21
26
8.1
L
34
5.1
L
17

LAB776
75749.1
—
—
—
—
—
—
4.8
0.06
9

LAB776
75750.3
68.6
L
43
8.6
L
43
5.2
L
20

LAB776
75750.4
64.1
L
33
8.0
L
33
5.0
0.02
15

LAB772
75459.1
54.1
0.02
12
6.8
0.02
12
4.6
0.22
5

LAB772
75461.6
52.6
0.04
9
6.6
0.04
9
—
—
—

LAB768
75453.1
63.3
0.08
31
7.9
0.08
31
5.0
0.05
16

LAB768
75453.2
55.4
L
15
6.9
L
15
4.7
0.01
8

LAB768
75453.3
60.0
0.08
25
7.5
0.08
25
4.8
0.12
10

LAB768
75455.1
58.5
0.07
22
7.3
0.07
22
4.8
L
10

LAB752
75168.1
59.6
0.05
24
7.4
0.05
24
4.8
0.02
11

LAB746
75507.2
58.2
L
21
7.3
L
21
4.8
L
11

LAB739
75444.4
61.0
0.04
27
7.6
0.04
27
4.9
L
12

LAB739
75445.1
59.7
L
24
7.5
L
24
4.9
0.02
12

LAB733
75158.2
53.2
0.07
11
6.7
0.07
11
4.5
0.22
4

LAB724
75142.1
56.9
0.10
18
7.1
0.10
18
4.7
0.09
8

LAB724
75143.2
63.0
L
31
7.9
L
31
5.1
L
16

LAB724
75145.1
53.6
0.02
11
6.7
0.02
11
4.6
0.06
6

LAB724
75146.2
69.6
0.15
45
8.7
0.15
45
5.2
0.09
20

LAB718
75437.2
—
—
—
—
—
—
4.7
0.10
8

LAB718
75439.2
60.4
L
26
7.5
L
26
4.9
0.01
13

LAB716
75429.3
64.0
L
33
8.0
L
33
5.0
L
16

LAB716
75430.5
59.7
L
24
8.0
0.11
33
4.9
0.03
14

LAB716
75431.2
56.3
0.11
17
7.0
0.11
17
—
—
—

LAB705
74879.2
62.7
L
30
7.8
L
30
4.9
L
14

LAB705
74879.3
51.2
0.12
6
6.4
0.12
6
4.5
0.24
3

LAB705
74881.1
55.9
0.03
16
7.0
0.03
16
4.7
0.08
8

LAB686
75364.1
54.7
0.02
14
6.8
0.02
14
—
—
—

LAB686
75364.4
56.6
0.25
18
7.1
0.25
18
—
—
—

LAB686
75364.6
—
—
—
—
—
—
4.5
0.23
3

LAB681
75420.4
58.4
L
21
7.3
L
21
4.8
L
11

LAB681
75421.4
51.9
0.15
8
6.5
0.15
8
—
—
—

CONT.
—
48.1
—
—
6.0
—
—
4.3
—
—

LAB800
75331.6
84.7
0.28
28
10.6
0.28
28
5.6
0.22
17

LAB791
75318.2
88.1
0.22
33
11.0
0.22
33
5.7
0.18
18

LAB769
75345.1
86.2
0.28
30
10.8
0.28
30
5.7
0.19
19

LAB704
75132.3
—
—
—
—
—
—
5.5
0.28
14

LAB704
75133.1
—
—
—
—
—
—
5.5
0.27
14

CONT.
—
66.2
—
—
8.3
—
—
4.8
—
—

LAB807
76240.1
104.3
0.21
18
13.0
0.21
18
6.3
0.16
8

LAB793
75751.2
103.3
0.07
17
12.9
0.07
17
6.1
0.16
5

LAB793
75755.1
107.8
0.06
22
13.5
0.06
22
6.4
0.08
10

LAB727
76387.3
—
—
—
13.7
0.24
24
—
—
—

LAB726
76382.1
—
—
—
—
—
—
6.2
0.11
7

LAB688
75829.1
107.4
0.18
21
13.4
0.18
21
6.5
0.13
11

LAB625
76472.1
95.3
0.22
8
11.9
0.22
8
—
—
—

CONT.
—
88.5
—
—
11.1
—
—
5.8
—
—

Table 168. “CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment; “p-val.”—p-value, L—p < 0.01.

TABLE 169

Genes showing improved plant performance at normal growth conditions under

regulation of At6669 promoter

RGR Of Leaf Number
RGR Of Plot
RGR Of Rosette

(number/day)
Coverage (cm²/day)
Diameter (cm/day)

Gene

P-
%

P-
%

P-
%

Name
Event #
Ave.
Val.
Incr.
Ave.
Val.
Incr.
Ave.
Val.
Incr.

LAB795
76491.2
0.76
0.28
17
9.75
0.19
27
—
—
—

LAB795
76491.3
—
—
—
9.28
0.28
21
—
—
—

LAB795
76494.3
—
—
—
9.50
0.23
23
—
—
—

LAB792
75878.3
—
—
—
9.75
0.17
27
—
—
—

LAB792
75880.4
0.86
0.04
33
9.73
0.18
26
—
—
—

LAB789
77085.3
—
—
—
9.36
0.26
22
—
—
—

LAB784
76468.3
—
—
—
9.58
0.2
25
—
—
—

LAB784
76470.2
—
—
—
9.54
0.21
24
—
—
—

LAB769
75309.1
—
—
—
9.51
0.22
24
—
—
—

LAB769
75348.4
—
—
—
9.87
0.14
28
—
—
—

LAB649
77044.2
—
—
—
9.62
0.23
25
—
—
—

LAB649
77045.3
—
—
—
10.22
0.1
33
0.50
0.28
16

LAB637
75105.8
—
—
—
10.74
0.05
40
—
—
—

LAB636
75100.3
—
—
—
10.09
0.11
31
—
—
—

LAB636
75104.1
0.79
0.23
22
10.97
0.07
43
0.51
0.23
18

CONT.
—
0.65
—
—
7.69
—
—
0.43
—
—

LAB839
75623.3
0.67
0.16
40
—
—
—
—
—
—

LAB839
75624.1
0.69
0.13
44
18.32
0.09
34
—
—
—

LAB809
75651.2
0.65
0.2
36
—
—
—
—
—
—

LAB809
75651.3
0.70
0.12
46
—
—
—
—
—
—

LAB783
75980.5
0.70
0.13
45
—
—
—
—
—
—

LAB762
75866.2
—
—
—
16.95
0.21
24
—
—
—

LAB740
75264.1
0.63
0.26
31
—
—
—
—
—
—

LAB686
75364.6
0.70
0.14
46
17.05
0.2
25
—
—
—

LAB681
75418.2
0.66
0.24
37
—
—
—
—
—
—

LAB681
75421.4
0.64
0.25
33
—
—
—
—
—
—

LAB665
75040.3
0.73
0.08
53
16.46
0.29
20
—
—
—

LAB665
75042.3
—
—
—
17.69
0.14
29
0.65
0.16
17

LAB655
75119.2
0.68
0.14
42
—
—
—
—
—
—

CONT.
—
0.48
—
—
13.66
—
—
0.55
—
—

LAB815
76026.1
—
—
—
—
—
—
0.53
0.21
11

LAB815
76030.1
—
—
—
11.57
0.23
18
—
—
—

LAB794
75881.2
—
—
—
11.34
0.29
16
—
—
—

LAB762
75870.3
0.86
0.23
15
—
—
—
—
—
—

LAB748
74972.3
0.93
0.05
25
—
—
—
—
—
—

LAB748
74976.2
—
—
—
11.36
0.29
16
—
—
—

LAB706
74802.2
—
—
—
—
—
—
0.52
0.29
9

LAB689
74756.2
0.86
0.22
15
—
—
—
—
—
—

LAB689
74758.4
—
—
—
12.66
0.06
29
0.54
0.17
12

LAB689
74760.1
—
—
—
12.36
0.09
26
0.55
0.09
15

LAB666
74840.3
0.85
0.26
14
—
—
—
—
—
—

LAB624
77106.1
0.90
0.11
20
—
—
—
—
—
—

LAB624
77108.5
0.86
0.26
15
—
—
—
—
—
—

CONT.
—
0.74
—
—
9.80
—
—
0.48
—
—

LAB837
76259.4
—
—
—
11.47
0.08
18
0.57
0.27
12

LAB800
75331.5
—
—
—
11.03
0.17
14
—
—
—

LAB786
76230.2
0.81
0.21
24
11.58
0.05
19
0.60
0.08
17

LAB778
75966.3
—
—
—
11.75
0.05
21
0.59
0.14
16

LAB776
75750.3
—
—
—
—
—
—
0.58
0.18
13

LAB776
75750.4
0.82
0.12
27
—
—
—
—
—
—

LAB772
75461.6
—
—
—
—
—
—
0.59
0.11
16

LAB768
75453.1
0.81
0.18
24
—
—
—
—
—
—

LAB768
75453.3
0.82
0.13
27
—
—
—
—
—
—

LAB752
75165.2
—
—
—
11.17
0.13
15
0.57
0.2
12

LAB746
75508.3
—
—
—
10.96
0.19
13
0.57
0.26
11

LAB739
75444.5
0.84
0.12
30
11.98
0.04
23
0.57
0.2
13

LAB733
75155.2
0.83
0.12
28
10.86
0.24
12
—
—
—

LAB733
75158.3
—
—
—
10.84
0.28
12
0.57
0.24
12

LAB718
75437.2
—
—
—
10.76
0.27
11
0.57
0.2
12

LAB716
75429.3
—
—
—
11.37
0.1
17
0.59
0.1
16

LAB716
75431.2
—
—
—
11.33
0.1
17
—
—
—

LAB686
75363.1
—
—
—
—
—
—
0.57
0.26
11

LAB681
75419.1
—
—
—
11.02
0.17
13
0.57
0.21
12

CONT.
—
0.65
—
—
9.72
—
—
0.51
—
—

LAB792
75876.3
—
—
—
12.86
0.29
17
—
—
—

LAB636
75104.1
—
—
—
14.45
0.06
32
—
—
—

CONT.
—
—
—
—
10.98
—
—
—
—
—

LAB801
75887.2
—
—
—
16.60
0.21
16
—
—
—

LAB788
76234.1
—
—
—
16.97
0.16
18
—
—
—

LAB704
75132.2
—
—
—
16.76
0.17
17
—
—
—

CONT.
—
—
—
—
14.36
—
—
—
—
—

LAB835
75896.3
—
—
—
8.27
0.02
31
0.45
0.22
12

LAB835
75896.4
0.67
0.22
22
7.81
0.08
24
0.45
0.26
11

LAB835
75896.5
—
—
—
—
—
—
0.45
0.25
13

LAB835
75900.1
—
—
—
7.78
0.09
24
0.46
0.14
15

LAB833
75466.1
—
—
—
8.34
0.02
33
0.52
L
29

LAB833
75467.4
0.68
0.22
24
8.03
0.09
28
—
—
—

LAB833
75468.4
0.66
0.3
20
—
—
—
—
—
—

LAB796
75322.1
—
—
—
8.63
0.01
37
0.47
0.08
18

LAB780
75771.2
0.67
0.25
21
7.43
0.17
18
—
—
—

LAB780
75771.3
—
—
—
7.74
0.09
23
0.44
0.3
10

LAB780
75773.3
0.70
0.14
26
7.82
0.07
24
—
—
—

LAB778
75967.2
0.74
0.06
34
8.57
0.01
36
0.46
0.17
13

LAB778
75969.2
—
—
—
7.42
0.17
18
0.44
0.29
10

LAB778
75969.4
0.69
0.16
24
—
—
—
—
—
—

LAB776
75746.2
0.72
0.09
31
8.05
0.04
28
0.50
0.02
23

LAB776
75749.1
—
—
—
7.42
0.18
18
0.46
0.18
13

LAB776
75750.3
0.70
0.12
27
9.06
L
44
0.50
0.01
25

LAB776
75750.4
0.70
0.13
27
8.42
0.01
34
0.47
0.09
16

LAB772
75459.1
0.69
0.2
24
—
—
—
—
—
—

LAB772
75461.6
0.70
0.14
26
—
—
—
—
—
—

LAB768
75453.1
—
—
—
8.27
0.03
31
0.47
0.08
18

LAB768
75453.2
0.67
0.24
21
7.33
0.2
17
0.46
0.13
15

LAB768
75453.3
—
—
—
7.82
0.07
24
—
—
—

LAB768
75455.1
—
—
—
7.77
0.08
23
0.46
0.18
13

LAB752
75168.1
0.69
0.17
25
7.73
0.09
23
0.45
0.23
12

LAB746
75507.2
—
—
—
7.64
0.1
21
0.45
0.25
11

LAB746
75508.3
0.69
0.17
25
—
—
—
0.44
0.3
10

LAB739
75444.4
—
—
—
7.99
0.05
27
0.46
0.12
15

LAB739
75445.1
—
—
—
7.80
0.07
24
0.46
0.14
15

LAB733
75154.3
0.66
0.27
19
—
—
—
—
—
—

LAB724
75142.1
—
—
—
7.50
0.14
19
—
—
—

LAB724
75143.2
—
—
—
8.18
0.03
30
0.47
0.12
16

LAB724
75146.2
0.67
0.24
22
9.15
L
45
0.49
0.03
22

LAB718
75437.2
—
—
—
7.61
0.13
21
0.45
0.25
11

LAB718
75439.2
—
—
—
7.99
0.04
27
0.46
0.13
14

LAB718
75439.3
—
—
—
7.20
0.26
15
—
—
—

LAB716
75429.3
—
—
—
8.37
0.02
33
0.48
0.05
19

LAB716
75430.5
—
—
—
7.83
0.08
24
0.47
0.07
18

LAB716
75431.2
0.76
0.04
37
7.42
0.17
18
—
—
—

LAB705
74879.2
—
—
—
8.15
0.03
30
0.46
0.18
13

LAB705
74879.3
0.68
0.19
24
—
—
—
—
—
—

LAB705
74881.1
—
—
—
7.26
0.23
15
—
—
—

LAB686
75364.1
—
—
—
7.27
0.24
16
0.45
0.21
12

LAB686
75364.4
0.68
0.21
23
7.36
0.2
17
—
—
—

LAB681
75420.4
0.72
0.1
30
7.70
0.09
22
0.47
0.09
16

LAB681
75421.4
0.71
0.13
28
—
—
—
—
—
—

CONT.
—
0.55
—
—
6.29
—
—
0.40
—
—

LAB800
75331.6
—
—
—
11.43
0.25
29
0.58
0.22
20

LAB791
75318.2
—
—
—
11.82
0.2
33
0.58
0.24
19

LAB769
75345.1
—
—
—
11.56
0.25
30
0.58
0.23
20

CONT.
—
—
—
—
8.87
—
—
0.49
—
—

LAB793
75755.1
—
—
—
16.33
0.17
23
—
—
—

LAB787
76755.5
0.61
0.01
82
—
—
—
—
—
—

LAB736
76368.2
0.49
0.23
45
—
—
—
—
—
—

LAB736
76370.1
0.51
0.1
52
—
—
—
—
—
—

LAB736
76370.3
0.46
0.22
38
—
—
—
—
—
—

LAB727
76387.3
—
—
—
—
—
—
0.74
0.19
17

LAB726
76382.1
—
—
—
15.92
0.27
20
—
—
—

LAB698
76201.1
0.46
0.24
38
—
—
—
—
—
—

LAB688
75829.1
—
—
—
16.35
0.16
23
—
—
—

LAB687
75043.1
0.47
0.24
38
—
—
—
—
—
—

LAB687
75046.4
—
—
—
17.11
0.14
29
—
—
—

LAB677
76528.1
0.50
0.14
47
—
—
—
—
—
—

LAB673
76533.1
0.47
0.28
41
—
—
—
—
—
—

LAB625
76472.1
0.47
0.23
41
—
—
—
—
—
—

CONT.
—
0.34
—
—
13.29
—
—
0.64
—
—

Table 169. “CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment; “p-val.”—p-value, L—p < 0.01.

Example 25
Evaluating Transgenic Arabidopsis Plant Growth Under Abiotic Stress as Well as Under Favorable Conditions by Seedlind Analyses

Assay 3: Seedling analysis of plants growth under osmotic stress [poly(ethylene glycol) (PEG)]—One of the consequences of drought is the induction of osmotic stress in the area surrounding the roots; therefore, in many scientific studies, PEG (e.g., 2.2% PEG) is used to simulate the osmotic stress conditions resembling the high osmolarity found during drought stress.

Assay 4: Seedling analysis of plants growth under high salinity conditions (NaCl)—High salinity is an abiotic stress that challenges the root systems of plants. Thus, an assay in which plants are grown under high salinity (110-120 mM NaCl) was conducted and plant performance in terms of shoot and root growth was evaluated.

Assay 5: Seedling analysis of plants growth under low and favorable nitrogen concentration levels—Low nitrogen is another abiotic stress that impact root growth and seedling growth. Therefore, an assay that examines plant performance under low (0.75 mM Nitrogen) and favorable (15 mM Nitrogen) nitrogen concentrations was performed.

Description of Experiment for Assays 3 and 4:

Surface sterilized seeds were sown in basal media [50% Murashige-Skoog medium (MS) supplemented with 0.8% plant agar as solidifying agent] in the presence of Kanamycin (for selecting only transgenic plants). After sowing, plates were transferred for 2-3 days for stratification at 4° C. and then grown at 25° C. under 12-hour light 12-hour dark daily cycles for 7 to 10 days. At this time point, seedlings randomly chosen were carefully transferred to plates containing either 2.2% PEG: 0.5 MS media (assay 3), 110-120 mM NaCl: 0.5 MS media (assay 4), or Normal growth conditions (0.5 MS media). Each plate contained 5 seedlings of the same transgenic event, and 3-4 different plates (replicates) for each event. For each polynucleotide of the invention at least four independent transformation events were analyzed from each construct. Plants expressing the polynucleotides of the invention were compared to the average measurement of the control plants (empty vector or GUS reporter gene under the same promoter) used in the same experiment.

Description of Experiment for Assay 5:

Surface sterilized seeds were sown in basal media [50% Murashige-Skoog medium (MS) supplemented with 0.8% plant agar as solidifying agent] in the presence of Kanamycin (used as a selecting agent). After sowing, plates were transferred for 2-3 days for stratification at 4° C. and then grown at 25° C. under 12-hour light 12-hour dark daily cycles for 7 to 10 days. At this time point, seedlings randomly chosen were carefully transferred to plates containing ½ MS media (15 mM N) for the normal nitrogen concentration treatment and 0.75 mM nitrogen for the low nitrogen concentration treatments. For experiments performed in T₂lines, each plate contained 5 seedlings of the same transgenic event, and 3-4 different plates (replicates) for each event. For each polynucleotide of the invention at least four-five independent transformation events were analyzed from each construct. For experiments performed in T₁lines, each plate contained 5 seedlings of 5 independent transgenic events and 3-4 different plates (replicates) were planted. In total, for T₁lines, 20 independent events were evaluated. Plants expressing the polynucleotides of the invention were compared to the average measurement of the control plants (empty vector or GUS reporter gene under the same promoter) used in the same experiment.

Digital imaging—A laboratory image acquisition system, which consists of a digital reflex camera (Canon EOS 300D) attached with a 55 mm focal length lens (Canon EF-S series), mounted on a reproduction device (Kaiser RS), which included 4 light units (4×150 Watts light bulb) and located in a darkroom, was used for capturing images of plantlets sawn in agar plates.

The image capturing process was repeated every 3-4 days starting at day 1 till day 10 (see for example the images in FIGS. 3A-F).

An image analysis system was used, which consists of a personal desktop computer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ 1.39 (Java based image processing program which was developed at the U.S. National Institutes of Health and freely available on the internet at Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/). Images were captured in resolution of 10 Mega Pixels (3888×2592 pixels) and stored in a low compression JPEG (Joint Photographic Experts Group standard) format. Next, analyzed data was saved to text files and processed using the JMP statistical analysis software (SAS institute).

Seedling analysis—Using the digital analysis seedling data was calculated, including leaf area, root coverage and root length.

The relative growth rate for the various seedling parameters was calculated according to the following Formulas XXVI (below) and XVII (above).

Relative growth rate of leaf area=Regression coefficient of leaf area along time course (measured in cm²per day). Formula XXVI:

At the end of the experiment, plantlets were removed from the media and weighed for the determination of plant fresh weight. Plantlets were then dried for 24 hours at 60° C., and weighed again to measure plant dry weight for later statistical analysis. Growth rate was determined by comparing the leaf area coverage, root coverage and root length, between each couple of sequential photographs, and results were used to resolve the effect of the gene introduced on plant vigor, under osmotic stress, as well as under optimal conditions. Similarly, the effect of the gene introduced on biomass accumulation, under osmotic stress as well as under optimal conditions, was determined by comparing the plants' fresh and dry weight to that of control plants (containing an empty vector or the GUS reporter gene under the same promoter). From every construct created, 3-5 independent transformation events are examined in replicates.

Statistical analyses—To identify genes conferring significantly improved tolerance to abiotic stresses or enlarged root architecture, the results obtained from the transgenic plants were compared to those obtained from control plants. To identify outperforming genes and constructs, results from the independent transformation events tested were analyzed separately. To evaluate the effect of a gene event over a control the data was analyzed by Student's t-test and the p value was calculated. Results were considered significant if p<0.1. The JMP statistics software package was used (Version 5.2.1, SAS Institute Inc., Cary, N.C., USA).

Experimental Results:

The genes listed in Table 170 improved plant biomass when grown at standard conditions. These genes produced larger plant biomass (plant fresh and dry weight) when grown under standard conditions, compared to control plants. Larger plant biomass under these growth conditions indicates the high ability of the plant to better metabolize the nutrients present in the medium. Plants were evaluated in T2 generation (Table 170). The genes were cloned under the regulation of a constitutive promoter (At6669; SEQ ID NO:10446). The evaluation of each gene was performed by testing the performance of different number of events. Some of the genes were evaluated in more than one seedling analysis resulting in positive results as well.

TABLE 170

Genes showing improved plant performance at Normal growth

conditions under regulation of At6669 promoter (T2 generation)

Gene

Dry Weight [mg]
Fresh Weight [mg]

Name
Event #
Ave.
P-Val.
% Incr.
Ave.
P-Val.
% Incr.

LAB799
77468.3
8.0
0.11
40
—
—
—

LAB738
75163.4
12.8
0.03
123
236.5
0.02
119

CONT.
—
5.7
—
—
108.0
—
—

LAB682
77859.1
7.6
0.11
153
—
—
—

CONT.
—
3.0
—
—
—
—
—

LAB823
77027.1
6.5
0.04
84
109.0
0.03
47

LAB782
76850.4
5.7
0.11
61
—
—
—

LAB751
76930.2
5.2
0.26
48
—
—
—

LAB717
76430.1
4.7
0.26
33
—
—
—

LAB703
76422.2
5.1
0.24
44
—
—
—

CONT.
—
3.5
—
—
74.0
—
—

LAB832
77332.1
6.5
L
72
101.6
L
34

LAB832
77332.4
7.3
L
93
120.3
0.02
59

LAB832
77333.7
5.3
0.01
39
98.9
0.04
31

LAB770
77763.2
4.9
0.05
28
91.1
0.22
21

LAB755
75172.7
6.4
0.15
68
135.3
0.02
79

LAB722
76464.4
—
—
—
98.7
0.29
31

LAB678
77821.4
5.1
L
35
94.0
0.15
24

LAB678
77823.3
6.3
0.22
66
104.9
L
39

LAB660
75841.3
5.5
0.20
43
90.1
0.06
19

LAB653
75116.3
4.5
0.26
18
98.8
0.27
31

LAB630
77786.2
5.2
0.07
36
—
—
—

CONT.
—
3.8
—
—
75.6
—
—

LAB834
75891.1
7.8
L
40
175.9
0.09
31

LAB834
75892.5
8.1
0.07
45
192.5
0.17
44

LAB807
76236.3
6.6
0.26
18
—
—
—

LAB807
76237.1
11.8
L
111
277.7
L
107

LAB726
76381.1
10.0
0.05
79
194.4
0.19
45

LAB726
76382.1
8.8
L
57
168.3
0.19
26

LAB726
76385.1
7.5
0.03
34
185.3
0.26
38

LAB696
75849.2
—
—
—
200.3
0.15
50

LAB696
75850.4
6.7
0.29
20
—
—
—

LAB687
75045.2
7.0
0.03
26
182.0
0.14
36

LAB687
75046.1
7.9
0.12
41
—
—
—

LAB687
75046.4
6.6
0.12
19
179.8
0.27
34

LAB687
75047.2
6.6
0.08
19
—
—
—

LAB673
76532.2
8.3
0.07
48
231.8
0.16
73

LAB673
76533.1
8.4
0.12
50
189.9
0.25
42

LAB673
76534.3
7.1
0.22
27
—
—
—

LAB650
75836.2
7.6
L
36
200.0
0.29
49

LAB650
75838.2
8.2
0.05
46
—
—
—

LAB650
75839.2
6.5
0.26
17
—
—
—

LAB626
74770.1
7.6
0.01
37
181.4
0.13
35

LAB626
74771.2
7.4
0.02
33
196.6
0.05
47

CONT.
—
5.6
—
—
133.9
—
—

LAB848
77339.2
6.6
0.07
69
125.8
0.28
42

LAB811
78053.6
4.9
0.19
26
111.7
0.20
26

LAB785
77997.3
6.5
0.03
65
—
—
—

LAB728
75151.2
6.2
0.29
59
—
—
—

CONT.
—
3.9
—
—
88.8
—
—

LAB813
76245.2
—
—
—
145.3
0.27
41

CONT.
—
—
—
—
103.1
—
—

LAB725
75857.2
—
—
—
134.5
0.23
61

LAB657
75733.4
8.2
0.14
93
138.9
0.27
66

CONT.
—
4.3
—
—
83.8
—
—

LAB721
76102.3
7.2
0.22
24
—
—
—

LAB696
75850.2
8.0
0.14
37
152.2
0.23
16

LAB654
76745.2
9.4
0.24
62
—
—
—

LAB651
76739.3
8.0
0.15
37
—
—
—

CONT.
—
5.8
—
—
131.2
—
—

LAB803
76760.1
3.6
0.11
45
—
—
—

LAB799
77466.1
4.2
0.04
71
100.5
0.11
41

LAB799
77470.3
5.2
L
111
143.4
L
101

LAB779
77308.1
4.9
L
98
—
—
—

LAB779
77309.3
4.2
0.02
69
121.4
0.02
70

LAB761
77291.1
6.1
L
146
135.9
0.11
91

LAB761
77292.8
—
—
—
142.2
0.20
99

LAB761
77295.2
4.5
L
79
151.2
0.30
112

LAB731
77131.2
6.3
L
154
113.5
0.16
59

LAB731
77134.1
5.7
0.10
128
119.6
0.20
68

LAB727
76386.1
4.6
0.06
85
—
—
—

LAB727
76386.2
—
—
—
120.4
0.22
69

LAB727
76386.4
5.9
L
139
147.6
0.08
107

LAB727
76387.3
3.7
0.03
50
—
—
—

LAB617
76336.1
4.8
L
94
128.4
0.16
80

LAB616
76732.4
6.6
L
164
142.2
L
100

LAB616
76732.6
3.1
0.28
27
—
—
—

LAB616
76732.8
3.7
0.08
50
123.8
0.07
74

CONT.
—
2.5
—
—
71.3
—
—

Table 170. “CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment; “p-val.”—p-value; L—p < 0.01.

The genes listed in Tables 171-172 improved leaf and root performance when grown at standard conditions. These genes produced larger leaf area and root biomass (leaf area, root length and root coverage) when grown under standard growth conditions, compared to control plants. Plants producing larger root biomass have better possibilities to absorb larger amount of water and nitrogen from soil. Plants producing larger leafs have better ability to produce assimilates. Plants were evaluated in T2 generation (Table 171) or T1 generation (Table 172). The genes were cloned under the regulation of a constitutive promoter (At6669; SEQ ID NO:10446). The evaluation of each gene was performed by testing the performance of different number of events. Some of the genes were evaluated in more than one seedling analysis resulting in positive results as well.

TABLE 171

Genes showing improved plant performance at Normal growth conditions under

regulation of At6669 promoter (T2 generation)

Leaf Area [cm²]
Roots Coverage [cm²]
Roots Length [cm]

Gene

P-
%

P-
%

P-
%

Name
Event #
Ave.
Val.
Incr.
Ave.
Val.
Incr.
Ave.
Val.
Incr.

LAB817
75756.5
0.607
0.25
24
9.392
0.07
43
7.073
0.05
12

LAB810
76287.1
—
—
—
8.715
0.10
32
—
—
—

LAB799
77468.3
0.639
0.10
31
9.902
0.03
50
7.286
0.13
15

LAB738
75163.4
0.835
0.12
71
11.803
0.20
79
7.086
0.29
12

CONT.
—
0.489
—
—
6.584
—
—
6.316
—
—

LAB849
75686.2
—
—
—
10.671
0.12
44
7.274
0.17
13

LAB849
75706.2
—
—
—
—
—
—
7.427
0.01
16

LAB813
76243.3
—
—
—
9.594
0.12
29
7.411
0.05
16

LAB773
78332.2
—
—
—
9.581
0.17
29
—
—
—

LAB770
77762.1
—
—
—
—
—
—
7.115
0.21
11

LAB691
74780.3
—
—
—
8.991
0.22
21
6.746
0.23
5

CONT.
—
—
—
—
7.425
—
—
6.413
—
—

LAB844
76787.5
—
—
—
—
—
—
6.167
0.12
12

LAB842
76350.2
—
—
—
—
—
—
6.752
0.04
22

LAB842
76350.3
—
—
—
6.261
0.13
44
6.447
0.15
17

LAB841
76804.2
—
—
—
6.615
0.12
53
6.364
0.21
15

LAB829
76441.5
—
—
—
—
—
—
6.146
0.22
11

LAB823
77027.1
0.549
0.04
40
5.836
0.16
35
—
—
—

LAB823
77028.2
—
—
—
—
—
—
6.740
0.04
22

LAB816
76717.2
—
—
—
—
—
—
6.195
0.14
12

LAB782
76850.4
0.483
0.26
23
—
—
—
6.480
0.06
17

LAB753
76440.2
—
—
—
—
—
—
6.111
0.16
11

LAB717
76428.1
—
—
—
6.080
0.13
40
6.808
0.04
23

LAB717
76428.4
—
—
—
—
—
—
6.708
0.03
21

LAB717
76430.1
0.483
0.25
23
5.581
0.29
29
6.229
0.27
13

LAB703
76422.2
0.493
0.18
25
—
—
—
6.322
0.08
14

LAB671
76411.5
—
—
—
5.536
0.21
28
6.404
0.06
16

LAB671
76411.6
0.443
0.19
13
—
—
—
—
—
—

LAB667
76097.3
—
—
—
—
—
—
6.127
0.19
11

LAB629
76896.2
0.464
0.10
18
—
—
—
—
—
—

LAB629
76899.2
—
—
—
—
—
—
6.319
0.08
14

CONT.
—
0.393
—
—
4.335
—
—
5.529
—
—

LAB749
77758.1
—
—
—
—
—
—
6.893
0.29
9

LAB749
77759.4
—
—
—
8.134
0.10
41
—
—
—

LAB682
77859.1
0.629
0.24
57
7.609
0.13
32
7.306
0.14
16

CONT.
—
0.401
—
—
5.760
—
—
6.307
—
—

LAB832
77332.1
0.577
0.02
44
8.519
0.07
82
6.795
0.15
20

LAB832
77332.4
0.635
L
59
7.713
0.13
65
—
—
—

LAB832
77333.7
0.529
0.03
32
7.228
L
54
6.685
0.14
18

LAB832
77333.8
—
—
—
—
—
—
6.263
0.15
10

LAB770
77762.1
0.534
0.02
33
6.495
0.03
39
6.417
0.07
13

LAB770
77763.1
—
—
—
5.614
0.09
20
6.212
0.19
10

LAB770
77763.2
0.526
L
31
—
—
—
—
—
—

LAB770
77765.2
0.503
0.05
26
—
—
—
—
—
—

LAB755
75171.1
—
—
—
6.615
0.03
41
6.869
L
21

LAB755
75172.3
0.443
0.28
11
5.257
0.17
12
6.198
0.20
9

LAB755
75172.7
0.565
0.04
41
6.693
L
43
6.287
0.20
11

LAB755
75175.1
0.479
0.30
20
—
—
—
—
—
—

LAB722
76465.2
0.483
0.02
21
5.241
0.18
12
—
—
—

LAB678
77821.4
0.480
0.11
20
5.652
0.19
21
—
—
—

LAB678
77823.3
0.619
0.08
55
6.960
L
49
6.870
0.01
21

LAB660
75841.3
0.516
0.30
29
6.426
0.06
37
—
—
—

LAB660
75841.5
—
—
—
—
—
—
6.465
0.04
14

LAB660
75841.6
—
—
—
5.369
0.24
15
6.285
0.14
11

LAB653
75116.3
0.502
0.04
25
6.051
0.01
29
6.382
0.08
13

LAB630
77786.2
0.535
0.06
34
7.268
0.12
55
6.686
0.02
18

CONT.
—
0.400
—
—
4.684
—
—
5.670
—
—

LAB834
75891.1
0.656
L
33
9.746
0.02
33
—
—
—

LAB834
75892.5
0.706
0.02
43
10.924
L
49
7.747
0.02
12

LAB834
75893.1
0.537
0.22
9
8.914
0.12
21
7.534
0.20
9

LAB834
75895.1
0.609
0.23
23
8.715
0.25
19
—
—
—

LAB834
75895.4
—
—
—
—
—
—
7.731
0.04
12

LAB807
76236.3
0.584
0.18
18
9.518
0.03
30
7.447
0.08
8

LAB807
76237.1
0.945
L
91
12.607
L
72
8.101
L
17

LAB807
76239.1
—
—
—
—
—
—
7.442
0.14
8

LAB807
76240.4
—
—
—
8.404
0.26
14
7.337
0.16
6

LAB726
76381.1
0.731
0.08
48
10.370
0.04
41
—
—
—

LAB726
76382.1
0.793
0.04
61
—
—
—
—
—
—

LAB726
76385.1
0.574
0.08
16
9.020
0.13
23
—
—
—

LAB696
75849.2
0.562
0.29
14
—
—
—
7.675
0.06
11

LAB696
75850.4
0.564
0.27
14
8.424
0.28
15
—
—
—

LAB687
75043.1
—
—
—
8.811
0.10
20
7.562
0.04
9

LAB687
75045.2
0.678
0.12
37
—
—
—
—
—
—

LAB687
75046.1
0.618
0.05
25
9.211
0.28
25
—
—
—

LAB687
75046.4
0.641
0.04
30
10.822
L
47
7.833
0.02
13

LAB687
75047.2
0.611
0.02
24
9.130
0.23
24
7.488
0.28
8

LAB673
76532.2
0.646
0.11
31
10.397
0.09
41
7.558
0.16
9

LAB673
76533.1
0.678
0.10
37
—
—
—
—
—
—

LAB673
76534.3
0.603
0.06
22
8.691
0.14
18
—
—
—

LAB650
75836.1
—
—
—
—
—
—
7.507
0.12
9

LAB650
75836.2
0.650
L
32
10.045
0.02
37
7.964
0.03
15

LAB650
75838.2
0.674
0.04
37
11.304
L
54
7.963
L
15

LAB650
75839.2
0.614
0.01
24
8.870
0.15
21
7.737
0.07
12

LAB626
74770.1
0.663
L
34
9.874
0.01
34
7.513
0.07
9

LAB626
74771.2
0.618
0.05
25
—
—
—
—
—
—

CONT.
—
0.494
—
—
7.349
—
—
6.908
—
—

LAB728
75151.2
—
—
—
7.053
0.28
53
—
—
—

CONT.
—
—
—
—
4.615
—
—
—
—
—

LAB813
76243.3
—
—
—
6.815
0.28
25
7.211
0.04
21

LAB813
76245.2
0.636
0.29
25
—
—
—
—
—
—

LAB714
75139.4
0.627
0.23
23
—
—
—
—
—
—

LAB631
74855.3
—
—
—
7.441
0.16
37
6.547
0.26
10

CONT.
—
0.509
—
—
5.441
—
—
5.949
—
—

LAB809
75652.3
—
—
—
5.866
0.27
20
—
—
—

LAB798
75616.2
—
—
—
6.006
0.17
23
6.467
0.07
13

CONT.
—
—
—
—
4.889
—
—
5.707
—
—

LAB820
76121.2
—
—
—
—
—
—
7.132
0.24
15

LAB820
76123.2
—
—
—
7.576
0.27
33
6.984
0.19
13

LAB725
75857.2
—
—
—
8.502
0.30
50
7.242
0.16
17

LAB725
75857.3
—
—
—
—
—
—
6.986
0.17
13

LAB725
75858.3
—
—
—
—
—
—
6.783
0.29
10

LAB657
75733.4
0.641
0.16
39
8.445
0.14
49
6.859
0.29
11

LAB638
76092.1
—
—
—
8.710
0.17
53
7.266
0.14
17

CONT.
—
0.463
—
—
5.681
—
—
6.185
—
—

LAB763
76262.1
0.700
0.17
30
10.551
0.20
40
7.339
0.05
18

LAB763
76264.3
—
—
—
—
—
—
6.674
0.17
7

LAB721
76102.3
—
—
—
9.848
0.03
31
7.728
L
24

LAB721
76105.3
—
—
—
—
—
—
6.792
0.24
9

LAB696
75850.2
0.651
0.24
21
—
—
—
6.954
0.25
11

LAB654
76745.2
0.746
0.18
39
9.833
0.14
30
7.007
0.04
12

LAB651
76739.3
0.666
0.28
24
—
—
—
—
—
—

LAB635
76082.1
—
—
—
—
—
—
7.139
0.01
14

LAB635
76085.2
—
—
—
8.573
0.23
14
7.176
0.11
15

CONT.
—
0.538
—
—
7.542
—
—
6.240
—
—

LAB803
76760.1
0.495
L
40
8.459
L
55
—
—
—

LAB799
77466.1
0.474
0.13
34
6.713
0.20
23
—
—
—

LAB799
77468.3
0.385
0.29
9
6.339
0.28
16
—
—
—

LAB799
77470.3
0.547
0.15
54
—
—
—
—
—
—

LAB779
77308.1
0.494
L
39
7.780
0.03
42
—
—
—

LAB779
77309.3
0.493
L
39
8.842
0.01
62
7.806
0.04
13

LAB779
77310.3
0.496
0.15
40
6.744
0.04
23
—
—
—

LAB761
77291.1
0.571
0.06
61
9.783
0.02
79
8.053
0.03
17

LAB761
77292.8
0.427
0.27
21
—
—
—
—
—
—

LAB761
77295.2
0.484
0.16
37
7.752
0.14
42
—
—
—

LAB731
77131.2
0.653
0.05
84
—
—
—
—
—
—

LAB731
77133.5
—
—
—
7.104
0.15
30
—
—
—

LAB731
77134.1
0.546
0.10
54
7.595
0.21
39
—
—
—

LAB727
76386.1
0.500
L
41
7.535
0.05
38
7.515
0.13
9

LAB727
76386.4
0.543
L
53
9.748
L
78
—
—
—

LAB727
76387.3
0.460
L
30
—
—
—
—
—
—

LAB727
76389.2
0.507
0.05
43
6.639
0.11
21
—
—
—

LAB617
76336.1
0.561
L
58
9.636
L
76
7.554
0.11
10

LAB616
76732.4
0.503
0.09
42
8.151
0.14
49
—
—
—

LAB616
76732.8
0.475
0.05
34
7.409
0.08
35
—
—
—

CONT.
—
0.354
—
—
5.469
—
—
6.898
—
—

LAB825
76394.1
—
—
—
—
—
—
7.939
0.13
6

LAB758
76490.4
0.691
0.23
25
12.982
0.18
34
8.283
0.07
10

LAB747
76799.1
—
—
—
—
—
—
8.018
0.09
7

CONT.
—
0.553
—
—
9.654
—
—
7.502
—
—

LAB620

—
—
—
—
—
—
4.328
0.15
15

CONT.

—
—
—
—
—
—
3.768
—
—

Table 171. “CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment; “p-val.”—p-value, L—p < 0.01.

TABLE 172

Genes showing improved plant performance at Normal growth conditions

under regulation of At6669 promoter (T1 generation)

Roots Coverage [cm²]

Gene Name
Ave.
P-Val.
% Incr.

LAB845
9.6
0.08
15

LAB702
9.4
0.19
13

LAB645
9.6
0.21
15

CONT.
8.3
—
—

Table 172. “CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment; “p-val.”—p-value, L- p < 0.01.

The genes listed in Tables 173-174 improved plant growth rate (leaf area, root length and root coverage growth rate) when grown at standard growth conditions. These produced plants that grew faster than control plants when grown under standard growth conditions. Plants showing fast growth rate show a better plant establishment in soil. Faster growth was observed when growth rate of leaf area and root length and coverage was measured. Plants were evaluated in T2 generation (Table 173) or T1 generation (Table 174). The genes were cloned under the regulation of a constitutive promoter (At6669; SEQ ID NO:10446). Evaluation of each gene was performed by testing the performance of different number of events. Some of the genes were evaluated in more than one seedling analysis resulting in positive results as well.

TABLE 173

Genes showing improved plant performance at Normal growth conditions under

regulation of At6669 promoter (T2 generation)

RGR Of Leaf Area
RGR Of Roots
RGR Of Root

(cm²/day)
Coverage (cm²/day)
Length (cm/day)

P-
%

P-
%

P-
%

Gene Name
Event #
Ave.
Val.
Incr.
Ave.
Val.
Incr.
Ave.
Val.
Incr.

LAB849
75686.2
—
—
—
1.300
0.03
48
0.706
0.11
17

LAB849
75706.2
—
—
—
1.374
0.04
57
0.687
0.16
14

LAB813
76243.3
—
—
—
1.159
0.06
32
—
—
—

LAB773
78332.2
—
—
—
1.130
0.13
29
—
—
—

LAB691
74780.3
—
—
—
1.089
0.20
24
—
—
—

LAB669_H7
77878.9
—
—
—
1.161
0.19
33
0.725
0.13
21

CONT.
—
—
—
—
0.875
—
—
0.601
—
—

LAB817
75756.5
0.059
0.21
31
1.074
0.11
35
—
—
—

LAB810
76287.1
—
—
—
1.020
0.20
29
—
—
—

LAB799
77468.3
0.062
0.14
38
1.170
0.05
48
0.686
0.30
11

LAB738
75163.4
0.078
0.03
74
1.373
0.03
73
—
—
—

LAB701
75123.4
—
—
—
1.133
0.14
43
—
—
—

CONT.
—
0.045
—
—
0.793
—
—
0.616
—
—

LAB783
75976.2
0.051
0.14
35
—
—
—
—
—
—

LAB749
77757.4
—
—
—
—
—
—
0.612
0.21
11

LAB749
77758.1
—
—
—
0.803
0.25
20
0.633
0.23
15

LAB749
77759.4
0.050
0.28
32
0.970
0.02
44
—
—
—

LAB682
77859.1
0.062
0.01
64
0.897
0.12
34
0.678
0.15
23

LAB682
77859.4
—
—
—
—
—
—
0.618
0.11
12

CONT.
—
0.038
—
—
0.671
—
—
0.550
—
—

LAB842
76350.2
—
—
—
—
—
—
0.551
L
23

LAB842
76350.3
—
—
—
0.724
0.10
45
—
—
—

LAB841
76804.2
—
—
—
0.739
0.10
48
0.552
0.09
23

LAB827
76794.3
—
—
—
—
—
—
0.512
0.19
15

LAB823
77027.1
0.052
0.06
36
0.650
0.18
30
—
—
—

LAB823
77028.2
—
—
—
—
—
—
0.583
0.01
31

LAB816
76717.2
—
—
—
—
—
—
0.519
0.13
16

LAB782
76850.4
0.051
0.10
32
0.769
0.14
54
0.599
L
34

LAB753
76440.2
—
—
—
—
—
—
0.504
0.28
13

LAB742
76434.2
—
—
—
0.767
0.27
53
—
—
—

LAB717
76428.1
—
—
—
0.674
0.14
35
0.567
L
27

LAB717
76428.4
—
—
—
0.717
0.20
43
0.558
0.02
25

LAB717
76430.1
0.046
0.25
20
—
—
—
—
—
—

LAB703
76422.2
0.049
0.15
28
0.632
0.26
26
0.553
0.03
24

LAB671
76411.5
—
—
—
—
—
—
0.542
0.05
21

LAB671
76411.6
—
—
—
—
—
—
0.567
0.03
27

LAB667
76097.3
—
—
—
—
—
—
0.534
0.18
20

LAB629
76896.2
0.045
0.21
19
—
—
—
—
—
—

LAB629
76899.2
—
—
—
—
—
—
0.511
0.13
14

CONT.
—
0.038
—
—
0.501
—
—
0.447
—
—

LAB832
77332.1
0.056
L
43
1.037
L
88
0.672
0.04
29

LAB832
77332.4
0.059
L
50
0.935
L
69
—
—
—

LAB832
77333.7
0.049
0.06
24
0.864
L
56
0.634
0.08
22

LAB770
77762.1
0.054
L
39
0.789
L
43
0.639
0.05
22

LAB770
77763.1
—
—
—
0.678
0.12
23
0.604
0.12
16

LAB770
77763.2
0.051
0.04
32
—
—
—
—
—
—

LAB770
77765.2
0.045
0.21
16
—
—
—
—
—
—

LAB755
75171.1
—
—
—
0.802
L
45
0.668
0.02
28

LAB755
75172.7
0.056
L
43
0.797
L
44
0.608
0.16
17

LAB755
75175.1
0.046
0.24
17
—
—
—
—
—
—

LAB722
76465.2
0.050
0.08
27
—
—
—
—
—
—

LAB678
77821.4
0.045
0.21
16
0.681
0.12
23
—
—
—

LAB678
77823.3
0.058
L
48
0.837
L
52
0.649
0.09
24

LAB660
75841.3
0.048
0.15
24
0.768
0.04
39
—
—
—

LAB660
75841.5
—
—
—
0.663
0.27
20
—
—
—

LAB653
75116.3
0.049
0.05
26
0.717
0.06
30
—
—
—

LAB630
77786.2
0.055
L
40
0.878
L
59
0.652
0.08
25

CONT.
—
0.039
—
—
0.552
—
—
0.522
—
—

LAB834
75891.1
0.064
L
32
1.140
0.02
33
—
—
—

LAB834
75892.5
0.069
L
41
1.289
L
50
0.678
0.26
10

LAB834
75893.1
—
—
—
1.035
0.15
21
0.705
0.12
14

LAB834
75895.1
0.058
0.25
20
—
—
—
—
—
—

LAB834
75895.4
—
—
—
—
—
—
0.708
0.10
15

LAB807
76236.3
0.059
0.12
20
1.117
0.04
30
—
—
—

LAB807
76237.1
0.091
L
87
1.435
L
67
0.680
0.25
10

LAB807
76239.1
—
—
—
—
—
—
0.685
0.19
11

LAB726
76381.1
0.074
L
52
1.215
0.01
42
—
—
—

LAB726
76382.1
0.079
L
61
1.287
0.08
50
—
—
—

LAB726
76385.1
0.058
0.09
19
1.033
0.16
21
—
—
—

LAB687
75043.1
—
—
—
1.010
0.20
18
—
—
—

LAB687
75045.2
0.068
0.02
39
—
—
—
—
—
—

LAB687
75046.1
0.064
0.02
31
1.069
0.18
25
—
—
—

LAB687
75046.4
0.060
0.08
23
1.258
L
47
0.678
0.28
10

LAB687
75047.2
0.061
0.03
25
1.057
0.17
23
0.683
0.28
11

LAB673
76532.2
0.063
0.05
30
1.218
0.02
42
0.679
0.29
10

LAB673
76533.1
0.066
0.03
35
—
—
—
—
—
—

LAB673
76534.3
0.062
0.04
27
1.046
0.12
22
—
—
—

LAB650
75836.2
0.063
0.02
30
1.162
0.03
36
0.696
0.19
13

LAB650
75838.2
0.070
L
43
1.332
L
55
0.731
0.04
19

LAB650
75839.2
0.060
0.05
22
1.025
0.18
20
0.698
0.17
13

LAB626
74770.1
0.062
0.03
27
1.141
0.02
33
—
—
—

LAB626
74771.2
0.062
0.03
28
—
—
—
—
—
—

CONT.
—
0.049
—
—
0.857
—
—
0.616
—
—

LAB848
77339.2
0.052
0.27
32
0.725
0.25
34
—
—
—

LAB728
75151.2
0.055
0.29
40
0.846
0.08
56
—
—
—

CONT.
—
0.039
—
—
0.541
—
—
—
—
—

LAB813
76243.3
—
—
—
0.791
0.26
26
0.613
0.30
15

LAB631
74855.3
—
—
—
0.875
0.11
40
—
—
—

CONT.
—
—
—
—
0.626
—
—
0.535
—
—

LAB798
75616.2
—
—
—
0.719
0.21
24
0.573
0.27
13

CONT.
—
—
—
—
0.582
—
—
0.508
—
—

LAB820
76121.2
—
—
—
—
—
—
0.670
0.16
24

LAB820
76123.2
—
—
—
0.898
0.19
33
0.647
0.11
19

LAB725
75857.2
—
—
—
1.027
0.18
52
0.678
0.11
25

LAB725
75857.3
—
—
—
0.897
0.21
33
0.661
0.06
22

LAB725
75858.3
—
—
—
—
—
—
0.623
0.21
15

LAB657
75733.4
0.064
0.23
42
1.005
0.17
49
—
—
—

LAB638
76092.1
—
—
—
1.049
0.09
55
—
—
—

LAB634
76087.1
—
—
—
—
—
—
0.642
0.25
18

CONT.
—
0.045
—
—
0.676
—
—
0.542
—
—

LAB763
76262.1
0.070
0.09
30
1.233
0.07
39
0.606
0.24
14

LAB763
76264.3
—
—
—
—
—
—
0.612
0.06
15

LAB721
76102.3
0.060
0.29
12
1.180
0.03
33
0.721
L
35

LAB696
75850.2
0.065
0.17
22
—
—
—
0.665
0.06
25

LAB654
76745.2
0.075
0.06
40
1.154
0.10
30
0.630
0.07
18

LAB651
76739.3
0.069
0.11
28
—
—
—
—
—
—

LAB635
76082.1
—
—
—
—
—
—
0.594
0.27
12

CONT.
—
0.054
—
—
0.887
—
—
0.532
—
—

LAB803
76760.1
0.048
L
36
1.016
L
58
—
—
—

LAB799
77466.1
0.047
0.09
31
0.797
0.15
24
—
—
—

LAB799
77468.3
—
—
—
0.754
0.29
18
—
—
—

LAB799
77470.3
0.057
L
58
—
—
—
—
—
—

LAB779
77308.1
0.050
L
40
0.930
0.01
45
—
—
—

LAB779
77309.3
0.052
L
46
1.059
L
65
0.712
0.05
18

LAB779
77310.3
0.049
L
37
0.787
0.17
23
—
—
—

LAB761
77291.1
0.058
L
64
1.170
L
82
0.736
0.09
22

LAB761
77292.8
0.041
0.25
14
—
—
—
—
—
—

LAB761
77295.2
0.050
L
40
0.918
0.02
43
—
—
—

LAB731
77131.2
0.065
L
82
0.883
0.12
38
—
—
—

LAB731
77133.5
—
—
—
0.851
0.08
33
—
—
—

LAB731
77134.1
0.055
L
55
0.920
0.05
43
—
—
—

LAB727
76386.1
0.047
L
31
0.885
0.04
38
—
—
—

LAB727
76386.2
—
—
—
0.764
0.30
19
—
—
—

LAB727
76386.4
0.054
L
52
1.164
L
81
—
—
—

LAB727
76387.3
0.046
L
27
—
—
—
—
—
—

LAB727
76389.2
0.051
L
42
0.783
0.19
22
—
—
—

LAB617
76336.1
0.056
L
57
1.155
L
80
—
—
—

LAB616
76732.4
0.052
L
46
0.972
L
52
—
—
—

LAB616
76732.6
0.048
0.02
36
0.845
0.13
32
—
—
—

LAB616
76732.8
0.047
0.02
30
0.889
0.04
39
—
—
—

CONT.
—
0.036
—
—
0.641
—
—
0.602
—
—

LAB758
76490.4
—
—
—
1.533
0.12
33
—
—
—

CONT.
—
—
—
—
1.154
—
—
—
—
—

Table 173. “CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment; “p-val.”—p-value, L—p < 0.01

TABLE 174

Genes showing improved plant performance at Normal growth conditions

under regulation ofAt6669 promoter (T1 generation)

RGR Of Roots Coverage (cm²/day)

Gene Name
Ave.
P-Val.
% Incr.

LAB845
1.124
0.22
14

LAB702
1.143
0.24
16

LAB645
1.142
0.30
16

CONT.
0.988
—
—

Table 174. “CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment; “p-val.”—p-value, L- p < 0.01.

The genes presented in Tables 175 showed a significant improvement in plant ABST. These genes produced larger plant biomass (plant fresh and dry weight) when grown under high salinity conditions (assay 4), compared to control plants. Larger plant biomass under this growth conditions indicates the high ability of the plant to better metabolize the nutrients present in the medium. Plants were evaluated in T2 generation (Table 175). The genes were cloned under the regulation of a constitutive promoter (At6669; SEQ ID NO:10446). The evaluation of each gene was performed by testing the performance of different number of events. Some of the genes were evaluated in more than one seedling analysis resulting in positive results as well.

TABLE 175

Genes showing improved plant performance at High

Salinity growth conditions under regulation

of At6669 promoter (T2 generation)

Dry Weight
FreshWeight

[mg]
[mg]

Gene

P-
%

P-
%

Name
Event #
Ave.
Val.
Incr.
Ave.
Val.
Incr.

LAB843
77710.5
4.7
0.24
18
—
—
—

LAB783
75977.3
—
—
—
102.3
0.28
13

LAB767
77704.2
4.9
0.26
23
128.9
0.07
42

LAB749
77759.4
5.1
0.04
27
120.0
0.03
32

LAB682
77856.4
5.0
0.18
24
149.7
0.02
65

LAB682
77859.1
6.0
L
50
121.0
0.03
34

LAB627
77694.1
—
—
—
110.5
0.10
22

LAB627
77695.3
—
—
—
188.8
L
108

CONT.
—
4.0
—
—
90.6
—
—

LAB832
77333.7
5.9
0.10
43
116.4
0.15
60

LAB755
75171.2
—
—
—
128.1
0.07
76

LAB660
75841.5
6.5
0.10
55
132.5
0.12
82

LAB630
77787.1
—
—
—
100.6
0.27
38

LAB630
77789.1
5.1
0.25
22
128.7
0.12
77

CONT.
—
4.2
—
—
72.7
—
—

LAB814
76705.3
7.7
0.08
63
151.9
0.20
41

LAB763
76262.1
6.8
0.14
44
144.1
0.26
34

LAB763
76264.3
7.5
0.09
57
—
—
—

CONT.
—
4.7
—
—
107.5
—
—

LAB836
75981.1
—
—
—
125.3
0.10
37

LAB771
75873.2
5.5
0.26
36
142.2
0.09
55

LAB661
75268.3
—
—
—
114.3
0.26
25

CONT.
—
4.0
—
—
91.6
—
—

LAB831
76251.3
—
—
—
76.1
0.18
23

LAB831
76255.1
10.4
0.13
247
180.4
0.19
193

LAB809
75651.3
4.8
0.15
59
98.5
0.08
60

LAB809
75652.3
6.0
0.03
99
114.7
0.02
86

LAB798
75616.1
—
—
—
73.9
0.22
20

LAB664
76224.4
—
—
—
104.2
0.07
69

LAB664
76224.6
—
—
—
76.4
0.16
24

LAB655
75119.5
3.9
0.27
31
87.4
0.06
42

LAB655
75120.1
4.8
0.24
60
100.3
0.23
63

LAB655
75121.3
4.2
0.29
39
96.7
0.03
57

LAB640
76266.4
4.9
0.23
62
—
—
—

CONT.
—
3.0
—
—
61.6
—
—

LAB616
76732.8
5.1
0.30
57
—
—
—

CONT.
—
3.2
—
—
—
—
—

“CONT.”—Control;

“Ave.”—Average;

“% Incr.” = % increment;

“p-val.”—p-value;

L—p < 0.01.

The genes presented in Table 176 showed a significant improvement in plant ABST. These genes produced larger leaf area and root biomass (leaf area, root length and root coverage) when grown under high salinity growth conditions (assay 4), compared to control plants. Plants producing larger root biomass have better possibilities to absorb larger amount of water from soil. Plants were evaluated in T2 generation (Table 176). The genes were cloned under the regulation of a constitutive promoter (At6669; SEQ ID NO:10446). The evaluation of each gene was performed by testing the performance of different number of events. Some of the genes were evaluated in more than one seedling analysis resulting in positive results as well.

TABLE 176

Genes showing improved plant performance at high salinity growth conditions under

regulation of At6669 promoter (T2 generation)

Leaf Area [cm²]
Roots Coverage [cm²]
Roots Length [cm]

P-
%

P-
%

P-
%

Gene Name
Event #
Ave.
Val.
Incr.
Ave.
Val.
Incr.
Ave.
Val.
Incr.

LAB820
76121.3
0.320
0.09
22
—
—
—
4.895
0.10
9

LAB725
75857.3
0.365
0.02
39
—
—
—
4.993
0.05
11

LAB725
75860.2
0.324
0.13
24
—
—
—
—
—
—

LAB657
75733.4
0.438
L
67
—
—
—
5.004
0.02
11

LAB638
76092.2
—
—
—
—
—
—
4.999
0.10
11

LAB634
76086.4
0.316
0.12
21
—
—
—
—
—
—

LAB634
76087.1
0.386
0.07
47
—
—
—
—
—
—

CONT.
—
0.262
—
—
—
—
—
4.491
—
—

LAB843
77707.2
0.445
0.09
46
—
—
—
—
—
—

LAB843
77710.4
0.435
0.30
43
5.921
0.02
41
—
—
—

LAB843
77710.5
0.386
0.13
27
5.934
0.17
42
—
—
—

LAB805
76116.1
0.343
0.02
13
—
—
—
—
—
—

LAB805
76117.4
0.421
L
39
5.366
0.06
28
—
—
—

LAB805
76120.4
0.433
0.29
43
—
—
—
—
—
—

LAB783
75976.2
0.418
0.05
38
6.673
0.03
59
5.145
0.04
13

LAB783
75977.3
0.388
0.27
28
—
—
—
—
—
—

LAB767
77704.2
0.476
0.03
57
6.389
L
52
5.581
L
22

LAB749
77756.2
0.438
L
44
4.819
0.24
15
—
—
—

LAB749
77757.4
0.495
0.03
63
6.199
L
48
—
—
—

LAB749
77758.1
0.395
0.05
30
6.607
0.07
58
5.586
0.03
23

LAB749
77759.4
0.523
0.12
72
8.949
0.02
113
6.170
L
35

LAB682
77856.4
0.539
L
77
7.506
0.07
79
5.454
0.15
20

LAB682
77859.1
0.509
L
68
7.564
0.02
80
5.366
L
18

LAB627
77694.1
0.342
0.02
13
5.665
0.17
35
—
—
—

LAB627
77695.3
0.654
0.08
115
9.146
0.08
118
5.501
0.15
21

CONT.
—
0.304
—
—
4.193
—
—
4.559
—
—

LAB814
76705.3
—
—
—
8.392
0.28
42
—
—
—

LAB763
76265.4
—
—
—
—
—
—
6.154
0.25
17

CONT.
—
—
—
—
5.927
—
—
5.255
—
—

LAB832
77333.8
—
—
—
7.269
0.22
30
5.383
0.27
13

LAB755
75171.2
—
—
—
—
—
—
5.131
0.14
8

CONT.
—
—
—
—
5.590
—
—
4.743
—
—

LAB836
75981.1
0.521
0.02
32
—
—
—
—
—
—

LAB813
76242.2
—
—
—
—
—
—
5.954
0.25
16

LAB771
75873.2
0.462
0.07
17
—
—
—
—
—
—

CONT.
—
0.395
—
—
—
—
—
5.135
—
—

LAB831
76255.1
0.489
0.15
72
—
—
—
5.393
0.28
20

LAB809
75651.3
—
—
—
5.408
0.29
25
5.368
0.03
19

LAB809
75652.3
0.387
0.04
36
—
—
—
—
—
—

LAB664
76224.4
0.374
0.06
31
—
—
—
—
—
—

LAB664
76224.6
—
—
—
—
—
—
4.940
0.18
10

LAB655
75119.5
0.351
0.12
23
—
—
—
—
—
—

LAB655
75120.1
—
—
—
5.988
0.23
39
—
—
—

LAB640
76266.4
0.373
0.06
31
—
—
—
4.860
0.28
8

CONT.
—
0.285
—
—
4.310
—
—
4.504
—
—

LAB616
76732.8
0.444
0.02
51
—
—
—
—
—
—

CONT.
—
0.294
—
—
—
—
—
—
—
—

“CONT.”—Control;

“Ave.”—Average;

“% Incr.” = % increment;

“p-val.”—p-value,

L—p < 0.01.

The genes presented in Table 177 showed a significant improvement in plant ABST. These genes improved plant growth rate (leaf area, root length and root coverage growth rate) when grown under high salinity growth conditions (assay 4), compared to control plants. Plants showing fast growth rate show a better plant establishment in soil. Faster growth was observed when growth rate of leaf area and root length and coverage was measured. Plants were evaluated in T2 generation (Table 177). The genes were cloned under the regulation of a constitutive promoter (At6669; SEQ ID NO:10446). Evaluation of each gene was performed by testing the performance of different number of events. Some of the genes were evaluated in more than one seedling analysis resulting in positive results as well.

TABLE 177

Genes showing improved plant performance at high salinity conditions

under regulation of At6669 promoter (T2 generation)

RGR Of Leaf Area
RGR Of Roots
RGR Of Root Length

(cm²/day)
Coverage (cm²/day)
(cm/day)

Gene

P-
%

P-
%

P-
%

Name
Event #
Ave.
Val.
Incr.
Ave.
Val.
Incr.
Ave.
Val.
Incr.

LAB820
76121.2
0.028
0.15
71
—
—
—
—
—
—

LAB820
76121.3
0.030
0.04
82
—
—
—
0.398
L
24

LAB725
75857.2
0.023
0.24
41
—
—
—
—
—
—

LAB725
75857.3
0.032
L
95
—
—
—
0.395
0.02
23

LAB725
75860.2
0.029
0.03
81
—
—
—
—
—
—

LAB657
75733.4
0.038
L
134
—
—
—
0.384
L
19

LAB638
76092.2
—
—
—
—
—
—
0.393
0.02
22

LAB634
76086.4
0.029
0.03
80
—
—
—
—
—
—

LAB634
76087.1
0.036
L
121
—
—
—
—
—
—

CONT.
—
0.016
—
—
—
—
—
0.321
—
—

LAB843
77707.2
0.041
0.02
51
—
—
—
—
—
—

LAB843
77710.4
0.040
0.02
47
0.706
0.07
42
—
—
—

LAB843
77710.5
0.033
0.18
22
0.704
0.07
41
—
—
—

LAB805
76117.4
0.040
L
48
0.651
0.17
31
—
—
—

LAB805
76120.4
0.039
0.03
45
—
—
—
—
—
—

LAB783
75976.2
0.039
L
44
0.809
L
62
0.469
0.04
23

LAB783
75977.3
0.035
0.12
31
—
—
—
—
—
—

LAB767
77704.2
0.046
L
70
0.761
L
53
0.449
0.08
17

LAB749
77756.2
0.039
L
45
—
—
—
—
—
—

LAB749
77757.4
0.046
L
69
0.742
0.04
49
0.438
0.25
15

LAB749
77758.1
0.036
0.03
32
0.790
0.01
59
0.468
0.04
22

LAB749
77759.4
0.048
L
80
1.077
L
116
0.566
L
48

LAB682
77856.4
0.050
L
87
0.894
L
79
0.486
0.08
27

LAB682
77859.1
0.048
L
78
0.901
L
81
0.456
0.05
19

LAB627
77694.1
0.032
0.19
19
0.669
0.14
34
—
—
—

LAB627
77695.3
0.063
L
135
1.103
L
121
0.497
0.02
30

CONT.
—
0.027
—
—
0.498
—
—
0.383
—
—

LAB814
76705.3
—
—
—
0.996
0.24
42
0.496
0.29
21

LAB763
76265.4
—
—
—
—
—
—
0.513
0.12
25

CONT.
—
—
—
—
0.702
—
—
0.412
—
—

LAB832
77333.8
—
—
—
0.876
0.21
29
—
—
—

CONT.
—
—
—
—
0.677
—
—
—
—
—

LAB836
75981.1
0.050
0.05
32
—
—
—
—
—
—

LAB771
75873.2
0.046
0.18
20
—
—
—
—
—
—

CONT.
—
0.038
—
—
—
—
—
—
—
—

LAB831
76255.1
0.043
0.02
71
0.761
0.20
50
0.459
0.16
24

LAB809
75651.3
—
—
—
0.639
0.20
26
0.430
0.21
16

LAB809
75652.3
0.033
0.10
33
—
—
—
—
—
—

LAB664
76224.4
0.034
0.07
35
0.725
0.22
42
0.475
0.13
28

LAB655
75119.5
0.032
0.10
28
—
—
—
—
—
—

LAB655
75120.1
0.042
0.17
68
0.719
0.17
41
—
—
—

LAB640
76266.4
0.032
0.16
27
—
—
—
—
—
—

CONT.
—
0.025
—
—
0.509
—
—
0.371
—
—

LAB616
76732.8
0.043
0.01
72
—
—
—
—
—
—

CONT.
—
0.025
—
—
—
—
—
—
—
—

“CONT.”—Control;

“Ave.”—Average;

“% Incr.” = % increment;

“p-val.”—p-value,

L—p < 0.01.

The genes presented in Tables 178-179 showed a significant improvement in plant NUE since they produced larger plant biomass (plant fresh and dry weight) in T2 generation (Table 178) or T1 generation (Table 179) when grown under limiting nitrogen growth conditions (assay 5), compared to control plants that were grown under identical growth conditions. Larger plant biomass under these growth conditions indicates the high ability of the plant to better metabolize the nutrients present in the medium. The genes were cloned under the regulation of a constitutive promoter (At6669; SEQ ID NO:10446). The evaluation of each gene was performed by testing the performance of different number of events. Some of the genes were evaluated in more than one seedling analysis resulting in positive results as well.

TABLE 178

Genes showing improved plant performance at Low Nitrogen growth

conditions under regulation of At6669 promoter (T2 generation)

Dry Weight [mg]
Fresh Weight [mg]

Gene Name
Event #
Ave.
P-Val.
% Incr.
Ave.
P-Val.
% Incr.

LAB849
75706.2
5.4
0.10
26
254.1
0.28
99

LAB836
75984.4
5.5
0.10
30
280.6
0.26
120

LAB773
78332.2
4.9
0.19
14
261.9
0.26
105

LAB771
75873.4
5.6
0.08
32
—
—
—

LAB771
75875.1
—
—
—
264.1
0.25
107

LAB727
76386.1
5.6
0.06
31
—
—
—

LAB727
76386.2
5.2
0.05
23
—
—
—

LAB727
76387.1
5.6
0.06
33
—
—
—

LAB702
78293.8
5.9
0.12
38
—
—
—

LAB700
77192.2
6.4
0.07
50
—
—
—

LAB691
74780.3
5.3
0.04
24
—
—
—

LAB691
74782.3
5.9
0.03
38
273.4
0.23
114

LAB688
75827.2
6.4
L
50
—
—
—

LAB669_H7
77878.9
5.5
0.03
28
—
—
—

LAB669_H7
77880.2
4.9
0.26
16
—
—
—

LAB661
75272.1
5.6
0.04
31
297.9
0.16
133

CONT.
—
4.3
—
—
127.7
—
—

LAB810
76287.1
6.0
0.27
23
97.2
0.06
27

LAB799
77468.3
—
—
—
105.3
0.06
37

LAB779
77306.4
7.7
0.26
60
—
—
—

LAB779
77308.1
—
—
—
89.3
0.16
16

LAB738
75163.4
—
—
—
92.5
0.24
21

LAB731
77131.2
—
—
—
90.2
0.12
18

LAB721
76102.3
—
—
—
89.3
0.09
16

LAB641
77225.2
6.9
0.12
44
108.0
0.11
41

LAB635
76084.5
—
—
—
113.1
0.18
47

LAB631
74856.6
—
—
—
111.4
0.10
45

CONT.
—
4.8
—
—
76.7
—
—

LAB844
76787.4
5.3
L
95
77.2
L
48

LAB844
76789.3
4.4
0.01
61
68.7
0.05
31

LAB842
76348.1
5.2
L
89
97.8
L
87

LAB842
76350.2
—
—
—
58.8
0.17
12

LAB842
76350.3
3.4
0.01
24
62.3
0.15
19

LAB841
76804.2
4.3
0.02
58
73.4
0.09
40

LAB841
76804.6
3.4
0.01
25
—
—
—

LAB829
76441.5
3.7
0.29
35
71.1
0.19
36

LAB829
76442.2
3.0
0.23
11
—
—
—

LAB829
76443.1
3.1
0.19
14
60.7
0.14
16

LAB827
76791.2
6.0
L
118
88.6
L
69

LAB827
76794.3
3.4
0.08
26
70.0
0.06
34

LAB823
77027.1
5.1
0.04
87
78.6
0.09
50

LAB823
77028.2
3.4
0.16
27
67.9
0.02
30

LAB816
76718.3
3.4
0.08
25
62.1
0.04
19

LAB816
76719.1
5.5
L
100
79.8
L
52

LAB782
76847.1
3.9
0.02
43
63.1
0.12
21

LAB782
76848.6
5.9
L
115
91.4
L
75

LAB782
76850.4
4.7
0.10
72
81.6
0.04
56

LAB753
76438.4
3.9
0.01
43
74.3
0.11
42

LAB753
76439.3
4.1
L
51
66.3
0.02
27

LAB751
76930.2
4.0
L
48
69.6
L
33

LAB742
76432.1
3.4
0.16
24
68.3
0.04
31

LAB742
76434.2
3.1
0.08
14
—
—
—

LAB742
76434.3
3.4
L
23
62.1
0.22
19

LAB717
76428.1
3.2
0.06
17
—
—
—

LAB717
76428.4
3.1
0.24
16
—
—
—

LAB717
76430.1
3.4
0.05
25
—
—
—

LAB703
76422.2
3.6
L
30
71.9
L
37

LAB703
76422.4
4.6
0.09
70
75.3
0.12
44

LAB703
76425.1
3.8
L
38
68.5
0.02
31

LAB671
76411.1
4.3
0.02
57
83.1
0.02
59

LAB671
76411.5
4.3
L
57
72.3
0.07
38

LAB667
76096.1
3.8
0.02
38
65.6
0.11
25

LAB629
76899.1
3.3
0.12
21
66.0
0.07
26

LAB629
76899.2
3.4
0.13
26
65.2
0.13
25

CONT.
—
2.7
—
—
52.3
—
—

LAB820
76121.1
6.1
0.08
34
183.4
0.13
101

LAB820
76121.2
5.5
0.19
22
105.0
0.20
15

LAB820
76122.2
6.1
0.06
35
138.6
0.11
52

LAB781
75334.1
5.3
0.25
18
109.5
0.13
20

LAB750
75447.2
5.6
0.20
24
—
—
—

LAB750
75448.1
—
—
—
144.6
0.25
58

LAB725
75857.1
—
—
—
117.7
0.17
29

LAB657
75732.2
5.6
0.09
23
181.5
0.17
99

LAB657
75732.5
—
—
—
126.2
L
38

LAB638
76092.1
6.7
L
48
116.2
0.06
27

CONT.
—
4.5
—
—
91.2
—
—

LAB834
75891.1
—
—
—
168.6
0.28
53

LAB834
75892.5
5.8
0.02
29
—
—
—

LAB834
75895.4
5.6
0.04
27
—
—
—

LAB807
76236.3
5.3
0.13
20
—
—
—

LAB807
76237.1
6.9
0.03
56
153.5
0.25
39

LAB807
76239.1
—
—
—
159.2
0.27
45

LAB726
76381.1
6.3
0.04
43
—
—
—

LAB726
76382.1
6.1
0.05
38
—
—
—

LAB696
75850.4
6.5
0.06
46
147.6
0.20
34

LAB687
75046.1
—
—
—
159.7
0.02
45

LAB687
75046.4
6.6
L
48
150.3
0.06
36

LAB650
75836.2
6.0
0.03
35
—
—
—

LAB650
75838.2
5.9
0.10
32
—
—
—

LAB650
75839.2
—
—
—
153.8
0.11
40

LAB626
74770.1
6.2
0.02
39
—
—
—

LAB626
74771.2
6.6
L
49
—
—
—

CONT.
—
4.5
—
—
110.1
—
—

LAB848
77339.2
5.2
0.10
33
88.0
0.07
22

LAB848
77339.4
5.0
0.08
27
80.4
0.30
11

LAB811
76345.4
4.5
0.09
17
91.2
0.04
26

LAB811
78053.6
—
—
—
87.4
0.26
21

LAB785
77997.3
4.5
0.09
16
—
—
—

LAB785
77998.1
4.8
0.06
24
—
—
—

LAB728
75150.2
4.5
0.23
15
84.8
0.18
17

LAB728
75151.2
—
—
—
90.8
0.10
26

LAB692
76418.1
—
—
—
82.4
0.21
14

LAB692
76418.4
—
—
—
88.8
0.05
23

LAB692
78068.2
4.9
0.01
25
81.0
0.25
12

LAB692
78069.7
—
—
—
86.0
0.27
19

LAB672
77698.1
5.0
0.01
29
81.9
0.25
13

LAB672
77698.3
5.0
0.04
29
89.0
0.05
23

LAB672
77699.1
—
—
—
85.1
0.21
18

LAB672
77699.2
4.8
0.04
23
94.5
0.03
31

LAB672
77700.1
5.0
L
28
88.5
0.07
23

LAB662
77296.3
5.2
0.02
32
94.4
0.07
31

LAB662
77299.2
5.0
0.03
29
81.8
0.25
13

LAB633
74828.2
5.5
0.02
40
91.1
0.12
26

LAB633
74828.4
4.4
0.24
12
95.9
0.03
33

CONT.
—
3.9
—
—
72.2
—
—

LAB850
77714.2
5.3
0.17
18
—
—
—

LAB850
77714.3
5.3
0.28
18
131.5
0.09
45

LAB825
76392.1
5.7
0.08
26
—
—
—

LAB793
75755.1
—
—
—
129.5
0.25
43

LAB765
76114.3
5.6
0.06
24
115.8
0.14
28

LAB760
75863.1
5.5
0.19
23
116.5
0.10
29

LAB760
75865.3
—
—
—
116.8
0.15
29

LAB758
76490.4
5.6
0.07
25
146.1
0.08
62

LAB747
76796.1
5.3
0.28
17
—
—
—

LAB735
77323.1
6.5
0.02
45
—
—
—

LAB735
77325.5
—
—
—
147.8
0.07
63

LAB723
77282.2
5.8
0.17
29
137.1
0.29
52

LAB723
77282.3
5.2
0.15
17
—
—
—

LAB711
77076.1
—
—
—
125.0
0.25
38

LAB711
77079.4
6.0
0.11
35
—
—
—

LAB710
76365.1
6.3
0.05
42
129.1
0.08
43

LAB698
76202.2
—
—
—
117.0
0.23
29

LAB698
76204.1
5.2
0.16
15
—
—
—

LAB625
76472.1
5.2
0.22
15
—
—
—

CONT.
—
4.5
—
—
90.4
—
—

LAB849
75706.2
—
—
—
77.1
0.25
16

LAB817
75758.1
5.5
0.29
22
83.3
0.14
25

LAB799
77468.1
—
—
—
85.3
0.22
28

LAB799
77470.3
—
—
—
81.4
0.23
22

LAB714
75137.2
5.5
0.13
21
75.3
0.15
13

LAB709
75427.8
5.2
0.26
13
84.2
0.05
27

LAB701
75123.4
5.5
0.26
20
87.8
0.02
32

LAB701
75124.1
5.8
0.19
28
81.7
0.04
23

LAB684
75278.4
—
—
—
75.0
0.24
13

LAB661
75272.1
5.5
0.28
21
78.0
0.24
17

LAB654
76742.3
5.2
0.23
15
89.6
0.05
35

LAB654
76745.1
5.8
0.21
27
73.0
0.28
10

LAB651
76738.2
—
—
—
76.1
0.19
14

LAB628
75499.1
—
—
—
83.1
0.05
25

CONT.
—
4.5
—
—
66.5
—
—

“CONT.”—Control;

“Ave.”—Average;

“% Incr.” = % increment;

“p-val.”—p-value,

L—p < 0.01.

TABLE 179

Genes showing improved plant performance at Low Nitrogen growth

conditions under regulation of At6669 promoter (T1 generation)

Dry Weight [mg]
Fresh Weight [mg]

Gene Name
Ave.
P-Val.
% Incr.
Ave.
P-Val.
% Incr.

LAB702
8.2
0.24
14
195.4
0.11
29

LAB647
7.9
0.21
10
—
—
—

LAB645
8.5
0.02
18
199.3
0.22
31

CONT.
7.2
—
—
151.8
—
—

LAB821
7.5
0.15
22
149.2
0.04
39

CONT.
6.1
—
—
107.5
—
—

“CONT.”—Control;

“Ave.”—Average;

“% Incr.” = % increment;

“p-val.”—p-value,

L—p < 0.01.

The genes presented in Tables 180-181 showed a significant improvement in plant NUE since they improved leaf and root performance when grown at low nitrogen conditions (assay 5). These genes produced larger leaf area and root biomass (leaf area, root length and root coverage) when grown under low nitrogen growth conditions, compared to control plants. Plants producing larger root biomass have better possibilities to absorb larger amount of water and nitrogen from soil. Plants producing larger leafs have better ability to produce assimilates. Plants were evaluated in T2 generation (Table 180) or T1 generation (Table 181). The genes were cloned under the regulation of a constitutive promoter (At6669; SEQ ID NO:10446). The evaluation of each gene was performed by testing the performance of different number of events. Some of the genes were evaluated in more than one seedling analysis resulting in positive results as well.

TABLE 180

Genes showing improved plant performance at Low Nitrogen growth

conditions under regulation of At6669 promoter (T2 generation)

Leaf
Roots
Roots

Area [cm²]
Coverage [cm²]
Length [cm]

P-
%

P-
%

P-
%

Gene Name
Event #
Ave.
Val.
Incr.
Ave.
Val.
Incr.
Ave.
Val.
Incr.

LAB817
75756.5
—
—
—
13.489
0.26
23
7.380
0.06
15

LAB817
75758.1
—
—
—
13.205
0.29
20
7.154
0.19
12

LAB810
76287.1
0.471
0.28
11
—
—
—
7.053
0.26
10

LAB803
76760.1
0.485
0.24
14
16.859
0.20
54
7.234
0.14
13

LAB799
77468.3
—
—
—
15.018
0.11
37
7.206
0.14
12

LAB779
77306.4
—
—
—
13.031
0.30
19
—
—
—

LAB779
77309.3
—
—
—
15.635
0.08
42
—
—
—

LAB738
75163.4
0.483
0.26
13
—
—
—
7.241
0.13
13

LAB721
76102.3
0.503
0.09
18
14.133
0.14
29
6.973
0.23
9

LAB641
77225.2
0.490
0.25
15
—
—
—
—
—
—

LAB631
74856.6
—
—
—
14.689
0.23
34
7.042
0.21
10

LAB617
76336.1
—
—
—
—
—
—
7.254
0.09
13

CONT.
—
0.426
—
—
10.973
—
—
6.408
—
—

LAB849
75706.2
—
—
—
14.097
0.03
23
—
—
—

LAB836
75984.4
—
—
—
13.140
0.14
14
—
—
—

LAB813
76243.3
—
—
—
12.626
0.21
10
—
—
—

LAB773
78332.2
—
—
—
13.788
0.13
20
7.493
0.14
5

LAB771
75873.2
—
—
—
12.865
0.13
12
—
—
—

LAB771
75873.4
—
—
—
13.329
0.24
16
—
—
—

LAB771
75875.1
0.521
0.27
6
—
—
—
—
—
—

LAB727
76386.1
0.569
0.03
16
14.528
0.02
26
7.764
L
8

LAB727
76386.2
—
—
—
14.075
0.17
22
—
—
—

LAB727
76387.1
—
—
—
14.026
L
22
—
—
—

LAB702
78293.8
0.598
0.14
22
14.508
0.19
26
7.579
0.19
6

LAB700
77192.2
0.551
0.04
13
16.202
0.10
41
—
—
—

LAB700
77195.3
—
—
—
13.565
0.05
18
—
—
—

LAB691
74782.3
0.540
0.11
10
13.916
0.15
21
—
—
—

LAB688
75827.2
—
—
—
14.166
0.10
23
—
—
—

LAB669_H7
77878.2
—
—
—
13.005
0.19
13
—
—
—

LAB669_H7
77878.9
0.536
0.16
10
14.635
0.05
27
7.643
0.18
7

LAB669_H7
77880.2
0.549
0.27
12
13.612
0.30
18
—
—
—

LAB628
75496.5
—
—
—
13.368
0.29
16
—
—
—

CONT.
—
0.490
—
—
11.493
—
—
7.163
—
—

LAB844
76787.4
0.471
L
47
9.195
0.16
47
—
—
—

LAB844
76789.3
0.389
0.03
21
8.157
0.25
31
—
—
—

LAB842
76348.1
0.510
L
59
11.503
L
84
7.463
L
24

LAB842
76350.2
0.346
0.21
8
—
—
—
6.702
0.08
12

LAB842
76350.3
0.380
0.02
19
7.319
0.28
17
—
—
—

LAB841
76804.2
0.436
0.01
36
8.628
0.08
38
6.464
0.18
8

LAB841
76804.6
0.386
L
20
7.227
0.05
16
6.509
0.06
8

LAB829
76441.5
0.420
0.17
31
9.470
0.08
52
7.050
0.02
18

LAB829
76442.2
0.376
L
17
7.832
L
25
6.732
L
12

LAB829
76443.1
0.357
0.07
11
7.775
0.04
24
—
—
—

LAB827
76791.2
0.430
0.22
34
13.957
L
123
7.432
L
24

LAB827
76794.3
0.399
0.02
24
7.698
0.06
23
6.475
0.12
8

LAB823
77026.4
0.390
0.26
22
—
—
—
—
—
—

LAB823
77027.1
0.453
L
41
10.460
0.04
68
6.665
0.06
11

LAB823
77028.2
0.403
0.03
26
8.458
0.02
35
7.315
L
22

LAB816
76717.2
0.384
0.09
20
8.092
0.13
30
6.747
0.06
12

LAB816
76718.3
0.350
0.13
9
9.385
L
50
6.638
0.02
11

LAB816
76719.1
—
—
—
12.716
0.01
104
7.175
L
20

LAB782
76848.6
0.478
L
49
12.055
L
93
7.217
L
20

LAB782
76850.4
0.467
0.01
46
9.867
L
58
7.119
L
19

LAB753
76438.4
0.428
0.07
34
—
—
—
—
—
—

LAB753
76439.3
0.398
L
24
8.250
0.04
32
—
—
—

LAB753
76440.2
—
—
—
7.286
0.08
17
—
—
—

LAB751
76928.2
0.372
0.09
16
—
—
—
—
—
—

LAB751
76929.2
0.342
0.19
7
7.652
0.02
23
6.560
0.02
9

LAB751
76930.2
0.435
L
36
8.470
L
36
6.425
0.05
7

LAB742
76432.1
0.381
0.15
19
—
—
—
—
—

LAB742
76434.3
0.378
0.05
18
—
—
—
—
—
—

LAB717
76428.1
0.374
0.02
17
7.748
0.15
24
6.926
L
15

LAB717
76428.4
0.373
0.23
16
8.190
0.12
31
6.739
0.08
12

LAB717
76430.1
0.412
0.02
29
7.364
0.29
18
—
—
—

LAB703
76422.2
0.386
0.03
20
7.385
0.18
18
6.540
0.22
9

LAB703
76422.4
0.472
0.08
47
11.313
0.06
81
7.173
0.04
20

LAB703
76425.1
0.407
0.01
27
7.494
0.23
20
6.428
0.29
7

LAB671
76411.1
0.458
L
43
8.991
0.13
44
6.848
0.19
14

LAB671
76411.5
0.446
L
39
11.056
L
77
7.049
L
17

LAB667
76096.1
0.459
L
43
9.440
0.04
51
6.622
0.10
10

LAB667
76097.3
0.349
0.27
9
7.724
0.01
24
6.471
0.05
8

LAB629
76896.2
0.387
0.23
21
—
—
—
—
—
—

LAB629
76899.1
0.379
0.03
18
8.452
0.15
35
6.588
0.06
10

LAB629
76899.2
0.429
L
34
7.866
0.03
26
6.900
0.02
15

CONT.
—
0.321
—
—
6.245
—
—
6.000
—
—

LAB820
76121.1
0.551
0.03
24
15.500
0.07
33
7.712
L
10

LAB820
76121.2
0.487
0.15
10
13.353
0.09
14
7.419
0.27
5

LAB820
76122.2
0.604
L
36
17.317
L
48
7.894
L
12

LAB781
75334.1
0.543
L
23
14.190
0.29
22
7.639
0.02
9

LAB781
75335.1
0.473
0.29
7
—
—
—
—
—
—

LAB755
75171.2
0.509
0.14
15
13.144
0.19
13
7.877
L
12

LAB750
75447.2
0.519
L
17
14.431
0.26
24
—
—
—

LAB750
75448.1
—
—
—
—
—
—
7.298
0.19
4

LAB747
76799.1
0.497
0.08
12
—
—
—
—
—
—

LAB725
75857.1
0.500
0.16
13
—
—
—
—
—
—

LAB725
75859.1
0.541
0.02
22
—
—
—
—
—
—

LAB657
75732.2
0.505
0.02
14
14.793
0.10
27
7.803
L
11

LAB657
75732.5
0.551
0.12
24
—
—
—
—
—
—

LAB638
76092.1
0.524
0.01
18
15.885
L
36
7.449
0.24
6

LAB638
76092.2
0.492
0.23
11
—
—
—
7.774
0.03
10

LAB638
76095.1
0.519
0.07
17
—
—
—
7.692
L
9

LAB623
75097.1
0.491
0.09
11
—
—
—
7.389
0.28
5

LAB617
76336.1
0.542
0.02
22
—
—
—
7.733
0.08
10

CONT.
—
0.444
—
—
11.671
—
—
7.036
—
—

LAB834
75891.1
0.531
0.04
26
12.885
0.02
26
7.129
0.06
8

LAB834
75892.5
0.506
0.04
20
15.302
0.06
50
7.366
0.03
11

LAB834
75893.1
0.514
0.08
22
12.167
0.13
19
—
—
—

LAB834
75895.1
0.505
0.03
20
11.194
0.26
10
—
—
—

LAB834
75895.4
0.491
0.23
17
13.794
0.06
35
7.284
0.05
10

LAB807
76236.3
0.486
0.03
15
12.459
0.16
22
—
—
—

LAB807
76237.1
0.555
L
32
15.978
0.05
57
7.415
0.02
12

LAB807
76239.1
0.506
0.01
20
13.500
0.06
32
7.496
L
13

LAB807
76240.4
0.473
0.01
12
—
—
—
—
—
—

LAB726
76381.1
0.475
L
13
15.821
L
55
7.430
L
12

LAB726
76382.1
0.606
0.07
44
13.801
0.08
35
7.524
L
14

LAB726
76382.2
0.441
0.30
5
—
—
—
—
—
—

LAB726
76385.1
0.486
L
15
—
—
—
7.419
L
12

LAB696
75849.2
0.542
0.05
29
11.300
0.26
11
7.353
0.17
11

LAB696
75850.2
—
—
—
12.764
L
25
7.192
0.13
9

LAB696
75850.4
0.503
0.02
20
17.559
0.02
72
7.829
L
18

LAB687
75043.1
—
—
—
12.099
0.03
19
7.115
0.05
8

LAB687
75046.1
0.498
0.02
18
13.567
0.09
33
7.368
0.04
11

LAB687
75046.4
0.512
0.02
21
16.421
L
61
7.357
0.02
11

LAB673
76533.1
0.440
0.22
5
—
—
—
—
—
—

LAB673
76540.2
0.495
0.10
17
—
—
—
—
—
—

LAB650
75836.1
0.461
0.04
10
—
—
—
—
—
—

LAB650
75836.2
0.486
0.03
15
13.521
0.01
32
6.994
0.03
6

LAB650
75838.1
—
—
—
12.076
0.04
18
6.931
0.30
5

LAB650
75838.2
0.482
0.14
14
14.586
0.04
43
7.394
L
12

LAB650
75839.2
0.471
0.06
12
11.902
0.07
17
7.025
0.07
6

LAB626
74770.1
—
—
—
14.036
0.11
37
7.242
0.14
10

LAB626
74771.2
0.475
L
13
15.173
L
49
7.038
0.04
6

LAB626
74771.3
—
—
—
—
—
—
6.960
0.17
5

CONT.
—
0.421
—
—
10.210
—
—
6.610
—
—

LAB848
77336.7
—
—
—
9.694
0.09
24
6.777
0.15
11

LAB848
77339.2
0.475
0.10
18
13.015
L
66
7.433
0.01
22

LAB848
77339.3
0.451
0.28
12
10.170
0.13
30
6.918
0.12
13

LAB848
77339.4
—
—
—
11.455
0.05
46
6.829
0.17
12

LAB811
76343.1
—
—
—
10.681
0.13
36
7.149
0.05
17

LAB811
76345.3
—
—
—
10.760
0.02
37
—
—
—

LAB811
76345.4
0.542
L
34
12.819
L
63
7.946
L
30

LAB811
78053.6
0.461
0.17
14
12.229
L
56
7.617
0.01
25

LAB811
78054.5
—
—
—
9.674
0.13
23
—
—
—

LAB785
77998.1
—
—
—
10.069
0.06
28
—
—
—

LAB728
75147.1
—
—
—
9.915
0.21
26
—
—
—

LAB728
75150.2
—
—
—
14.093
0.02
80
6.898
0.13
13

LAB728
75151.2
—
—
—
12.036
0.17
53
6.996
0.16
14

LAB728
75152.2
—
—
—
10.154
0.07
29
—
—
—

LAB692
76418.1
0.469
0.14
16
—
—
—
—
—
—

LAB692
76418.4
—
—
—
9.034
0.27
15
—
—
—

LAB692
78068.2
0.535
0.01
32
14.228
L
81
7.942
L
30

LAB692
78069.5
—
—
—
—
—
—
6.824
0.16
12

LAB692
78069.7
0.462
0.21
14
11.533
0.07
47
6.914
0.10
13

LAB676
77271.2
—
—
—
9.733
0.22
24
6.768
0.17
11

LAB676
77275.1
—
—
—
10.240
0.19
31
—
—
—

LAB676
77275.3
0.450
0.30
12
10.579
0.05
35
7.022
0.07
15

LAB672
77698.1
—
—
—
12.177
L
55
7.053
0.05
15

LAB672
77698.3
—
—
—
12.196
L
56
7.011
0.06
15

LAB672
77699.1
0.474
0.12
17
10.182
0.06
30
6.584
0.30
8

LAB672
77699.2
0.547
L
35
11.043
0.02
41
7.325
0.02
20

LAB672
77700.1
0.536
L
33
11.200
0.11
43
7.272
0.03
19

LAB662
77296.3
0.522
0.01
29
11.806
L
51
7.298
0.02
19

LAB662
77298.3
—
—
—
11.613
0.02
48
6.662
0.24
9

LAB662
77299.2
—
—
—
13.283
L
69
6.973
0.10
14

LAB633
74828.2
0.526
0.03
30
15.578
L
99
7.657
L
25

LAB633
74828.4
0.511
0.02
27
10.402
0.07
33
6.780
0.15
11

LAB633
74831.4
—
—
—
11.600
0.13
48
7.367
0.05
21

LAB633
74832.1
—
—
—
10.095
0.11
29
—
—
—

LAB633
74833.2
—
—
—
9.544
0.16
22
—
—
—

LAB622
75036.1
—
—
—
10.027
0.06
28
—
—
—

CONT.
—
0.404
—
—
7.841
—
—
6.110
—
—

LAB850
77714.2
—
—
—
13.837
0.21
25
—
—
—

LAB850
77714.3
0.486
0.11
18
14.480
0.10
31
—
—
—

LAB825
76392.1
—
—
—
15.126
0.07
37
—
—
—

LAB825
76394.1
—
—
—
13.788
0.08
25
7.483
0.12
7

LAB793
75753.1
—
—
—
13.411
0.16
21
—
—
—

LAB765
76113.3
0.476
0.10
15
14.622
0.03
32
7.491
0.21
7

LAB765
76113.5
0.493
0.18
20
16.378
0.05
48
—
—
—

LAB765
76114.3
0.582
L
41
14.719
0.06
33
—
—
—

LAB760
75862.2
—
—
—
12.615
0.27
14
—
—
—

LAB760
75865.3
0.491
0.06
19
—
—
—
—
—
—

LAB758
76490.2
0.492
0.09
19
—
—
—
—
—
—

LAB758
76490.4
0.522
0.02
27
15.459
0.01
40
7.690
0.09
10

LAB754
77289.3
0.465
0.24
13
—
—
—
—
—
—

LAB747
76796.1
—
—
—
14.309
0.12
29
7.485
0.13
7

LAB747
76799.1
0.510
0.09
24
—
—
—
—
—
—

LAB735
77323.1
0.465
0.26
13
15.428
L
39
—
—
—

LAB723
77282.2
0.513
0.02
24
15.411
0.01
39
7.997
0.06
14

LAB723
77282.3
—
—
—
13.855
0.11
25
—
—
—

LAB711
77077.3
—
—
—
15.600
0.19
41
—
—
—

LAB711
77079.4
0.474
0.17
15
15.173
0.02
37
—
—
—

LAB710
76362.2
0.484
0.16
17
13.536
0.23
22
—
—
—

LAB710
76365.1
0.527
0.07
28
14.684
0.09
33
7.659
0.14
9

LAB698
76202.2
0.461
0.25
12
—
—
—
—
—
—

LAB698
76204.1
0.458
0.22
11
—
—
—
—
—
—

LAB663
77048.1
—
—
—
14.175
0.06
28
7.474
0.17
6

LAB656
77316.1
—
—
—
—
—
—
7.638
0.06
9

LAB656
77320.5
—
—
—
14.043
0.07
27
—
—
—

LAB625
76451.1
0.482
0.08
17
—
—
—
—
—
—

LAB625
76452.1
—
—
—
15.092
0.20
36
—
—
—

LAB625
76472.1
0.486
0.13
18
13.634
0.08
23
—
—
—

CONT.
—
0.412
—
—
11.066
—
—
7.018
—
—

LAB817
75756.5
—
—
—
—
—
—
6.446
0.07
11

LAB810
76287.1
0.461
0.29
17
12.056
0.16
41
6.646
0.09
15

LAB799
77468.3
0.447
0.10
13
11.681
0.16
37
6.744
0.04
16

LAB738
75163.2
0.438
0.29
11
—
—
—
6.594
0.03
14

LAB714
75137.2
0.458
0.18
16
12.210
0.01
43
6.876
0.01
19

LAB709
75427.8
0.439
0.24
11
10.770
0.29
26
6.719
0.04
16

LAB701
75123.4
0.487
0.05
23
13.244
0.15
55
6.666
0.06
15

LAB701
75124.1
0.451
0.03
14
13.271
0.11
55
6.521
0.13
13

LAB684
75274.2
—
—
—
—
—
—
6.946
0.05
20

LAB661
75272.1
—
—
—
13.001
0.01
52
6.691
0.03
15

LAB654
76742.3
—
—
—
11.114
0.05
30
6.439
0.08
11

LAB654
76745.1
0.439
0.13
11
—
—
—
6.364
0.19
10

LAB631
74856.6
—
—
—
10.699
0.22
25
—
—
—

LAB628
75496.5
—
—
—
10.708
0.25
25
6.613
0.06
14

LAB628
75499.1
0.466
0.06
18
12.502
0.02
46
6.861
0.01
18

CONT.
—
0.395
—
—
8.543
—
—
5.794
—
—

“CONT.”—Control;

“Ave.”—Average;

“% Incr.” = % increment;

“p-val.”—p-value,

L—p < 0.01.

TABLE 181

Genes showing improved plant performance at Low Nitrogen

growth conditions under regulation of At6669 promoter

(T1 generation)

Leaf
Roots
Roots

Area [cm²]
Coverage [cm²]
Length [cm]

Gene

P-
%

P-
%

P-
%

Name
Ave.
Val.
Incr.
Ave.
Val.
Incr.
Ave.
Val.
Incr.

LAB745
0.525
L
17
15.341
0.19
37
6.741
0.06
11

LAB734
0.499
0.29
11
—
—
—
—
—
—

LAB685
0.533
0.13
19
—
—
—
—
—
—

CONT.
0.449
—
—
11.159
—
—
6.047
—
—

“CONT.”—Control;

“Ave.”—Average;

“% Incr.” = % increment;

“p-val.”—p-value,

L—p < 0.01.

The genes presented in Tables 182-183 showed a significant improvement in plant NUE. These genes improved plant growth rate (leaf area, root length and root coverage growth rate) when grown under low nitrogen growth conditions (assay 5), compared to control plants. Plants showing fast growth rate show a better plant establishment in soil. Faster growth was observed when growth rate of leaf area and root length and coverage was measured. Plants were evaluated in T2 generation (Table 182) or T1 generation (Table 183). The genes were cloned under the regulation of a constitutive promoter (At6669; SEQ ID NO:10446). Evaluation of each gene was performed by testing the performance of different number of events. Some of the genes were evaluated in more than one seedling analysis resulting in positive results as well.

TABLE 182

Genes showing improved plant performance at Low Nitrogen growth

conditions under regulation of At6669 promoter (T2 generation)

RGR Of Leaf Area
RGR Of Roots
RGR Of Root

(cm²/day)
Coverage (cm²/day)
Length (cm/day)

P-
%

P-
%

P-
%

Gene Name
Event #
Ave.
Val.
Incr.
Ave.
Val.
Incr.
Ave.
Val.
Incr.

LAB803
76760.1
—
—
—
2.026
0.08
54
—
—
—

LAB799
77468.3
—
—
—
1.804
0.12
37
—
—
—

LAB779
77309.3
—
—
—
1.895
0.08
44
—
—
—

LAB721
76102.3
—
—
—
1.713
0.18
30
—
—
—

LAB631
74856.6
—
—
—
1.784
0.17
36
—
—
—

CONT.
—
—
—
—
1.314
—
—
—
—
—

LAB849
75706.2
—
—
—
1.699
0.04
22
—
—
—

LAB836
75984.4
—
—
—
1.611
0.13
16
—
—
—

LAB773
78332.2
—
—
—
1.660
0.09
19
—
—
—

LAB771
75873.2
—
—
—
1.574
0.18
13
—
—
—

LAB771
75873.4
—
—
—
1.614
0.18
16
—
—
—

LAB727
76386.1
0.051
0.29
11
1.759
L
27
—
—
—

LAB727
76386.2
—
—
—
1.723
0.06
24
—
—
—

LAB727
76387.1
—
—
—
1.710
0.02
23
—
—
—

LAB702
78293.8
0.057
0.06
25
1.750
0.05
26
—
—
—

LAB700
77192.2
—
—
—
2.004
L
44
0.780
0.02
13

LAB700
77194.1
—
—
—
1.610
0.18
16
—
—
—

LAB700
77195.3
—
—
—
1.654
0.08
19
—
—
—

LAB691
74782.3
—
—
—
1.638
0.12
18
—
—
—

LAB688
75827.2
—
—
—
1.710
0.04
23
—
—
—

LAB684
75274.2
—
—
—
1.566
0.25
13
—
—
—

LAB669_H7
77878.2
—
—
—
1.601
0.16
15
—
—
—

LAB669_H7
77878.9
—
—
—
1.784
0.01
28
0.750
0.15
9

LAB669_H7
77880.2
—
—
—
1.671
0.12
20
—
—
—

LAB661
75272.1
—
—
—
1.610
0.22
16
—
—
—

LAB628
75496.5
—
—
—
1.621
0.21
17
—
—
—

CONT.
—
0.045
—
—
1.390
—
—
0.688
—
—

LAB844
76787.4
0.043
L
43
1.094
0.03
46
—
—
—

LAB844
76789.3
0.037
0.14
22
0.984
0.07
32
—
—
—

LAB842
76348.1
0.044
L
47
1.352
L
81
0.627
0.02
19

LAB842
76350.2
—
—
—
—
—
—
0.598
0.16
13

LAB842
76350.3
0.035
0.25
15
—
—
—
—
—
—

LAB841
76804.2
0.040
0.04
32
0.997
0.04
33
—
—
—

LAB841
76804.6
0.038
0.06
25
0.881
0.19
18
0.627
0.02
19

LAB829
76441.5
0.038
0.21
24
1.104
0.02
48
0.590
0.22
12

LAB829
76442.2
—
—
—
0.933
0.06
25
0.577
0.24
9

LAB829
76443.1
—
—
—
0.931
0.08
24
—
—
—

LAB827
76791.2
0.037
0.25
22
1.668
L
123
0.666
L
26

LAB827
76794.3
0.039
0.05
27
0.922
0.11
23
0.600
0.14
13

LAB823
77027.1
0.039
0.05
30
1.246
L
67
0.585
0.22
11

LAB823
77028.2
0.040
0.02
33
0.991
0.03
32
0.642
L
22

LAB816
76717.2
0.036
0.26
17
0.970
0.06
30
0.590
0.21
12

LAB816
76718.3
—
—
—
1.129
L
51
0.597
0.11
13

LAB816
76719.1
—
—
—
1.510
L
102
0.607
0.08
15

LAB782
76848.6
0.043
L
43
1.445
L
93
0.664
L
26

LAB782
76850.4
0.046
L
53
1.188
L
59
0.659
L
25

LAB753
76438.4
0.036
0.25
18
—
—
—
—
—
—

LAB753
76439.3
0.035
0.20
16
1.012
0.03
35
0.589
0.20
11

LAB753
76440.2
—
—
—
0.867
0.22
16
—
—
—

LAB751
76929.2
—
—
—
0.901
0.12
20
—
—
—

LAB751
76930.2
0.039
0.04
29
0.969
0.04
30
—
—
—

LAB742
76434.3
0.037
0.12
21
—
—
—
—
—
—

LAB717
76428.1
—
—
—
0.913
0.12
22
0.574
0.28
9

LAB717
76428.4
—
—
—
0.950
0.10
27
0.591
0.17
12

LAB717
76430.1
0.038
0.07
25
—
—
—
—
—
—

LAB703
76422.2
—
—
—
0.874
0.23
17
0.577
0.29
9

LAB703
76422.4
0.045
0.01
47
1.324
L
77
0.647
0.03
22

LAB703
76425.1
0.039
0.05
27
0.889
0.20
19
—
—
—

LAB671
76411.1
0.039
0.07
28
1.034
0.03
38
0.588
0.29
11

LAB671
76411.5
0.041
0.01
34
1.288
L
72
0.620
0.04
17

LAB667
76096.1
0.042
L
39
1.106
L
48
—
—
—

LAB667
76097.3
—
—
—
0.920
0.10
23
0.574
0.29
9

LAB629
76899.1
0.036
0.15
18
1.023
0.03
37
0.585
0.19
11

LAB629
76899.2
0.041
0.01
35
0.944
0.05
26
0.593
0.14
12

CONT.
—
0.030
—
—
0.748
—
—
0.529
—
—

LAB834
75891.1
0.052
L
32
1.517
0.03
26
—
—
—

LAB834
75892.5
0.046
0.06
17
1.846
L
54
0.674
0.01
16

LAB834
75893.1
0.048
0.04
22
1.445
0.10
20
—
—
—

LAB834
75895.1
0.048
0.02
22
—
—
—
0.617
0.26
6

LAB834
75895.4
0.047
0.11
19
1.656
L
38
0.647
0.09
12

LAB807
76236.3
0.046
0.05
17
1.488
0.07
24
—
—
—

LAB807
76237.1
0.045
0.14
14
1.922
L
60
0.651
0.06
12

LAB807
76239.1
0.049
0.02
24
1.607
0.02
34
0.685
L
18

LAB807
76240.4
0.044
0.20
11
1.365
0.28
14
—
—
—

LAB726
76381.1
—
—
—
1.897
L
58
0.633
0.09
9

LAB726
76382.1
0.054
L
36
1.643
0.01
37
0.649
0.05
12

LAB726
76385.1
0.050
L
25
—
—
—
0.692
L
19

LAB696
75849.2
0.051
L
29
—
—
—
—
—
—

LAB696
75850.2
—
—
—
1.524
0.03
27
0.653
0.05
13

LAB696
75850.4
0.043
0.28
9
2.116
L
76
0.710
L
22

LAB687
75043.1
—
—
—
1.442
0.09
20
—
—
—

LAB687
75046.1
0.046
0.09
15
1.600
0.02
33
0.634
0.13
9

LAB687
75046.4
—
—
—
1.942
L
62
—
—
—

LAB687
75047.2
—
—
—
1.410
0.24
18
0.614
0.30
6

LAB673
76533.1
—
—
—
1.380
0.28
15
0.651
0.07
12

LAB673
76540.2
0.045
0.14
14
—
—
—
—
—
—

LAB650
75836.1
0.044
0.14
11
—
—
—
—
—
—

LAB650
75836.2
—
—
—
1.614
L
35
0.627
0.10
8

LAB650
75838.1
—
—
—
1.456
0.08
21
0.620
0.28
7

LAB650
75838.2
0.046
0.08
17
1.764
L
47
0.658
0.02
13

LAB650
75839.2
0.046
0.07
15
1.411
0.13
18
0.616
0.23
6

LAB626
74770.1
—
—
—
1.692
L
41
0.649
0.05
12

LAB626
74771.2
—
—
—
1.837
L
53
0.671
L
16

CONT.
—
0.040
—
—
1.199
—
—
0.580
—
—

LAB848
77336.5
—
—
—
—
—
—
0.642
0.21
16

LAB848
77336.7
—
—
—
1.186
0.14
26
0.697
0.04
26

LAB848
77339.2
0.046
0.29
18
1.572
L
67
0.678
0.07
22

LAB848
77339.3
—
—
—
1.236
0.11
31
0.657
0.13
19

LAB848
77339.4
—
—
—
1.387
0.02
47
—
—
—

LAB811
76343.1
—
—
—
1.268
0.09
34
—
—
—

LAB811
76345.3
—
—
—
1.314
0.04
39
0.652
0.14
18

LAB811
76345.4
0.054
0.03
40
1.561
L
65
0.753
L
36

LAB811
78053.6
0.046
0.23
20
1.494
L
58
0.729
0.01
32

LAB811
78054.5
—
—
—
1.187
0.16
26
0.632
0.24
14

LAB785
77997.3
—
—
—
1.184
0.23
26
—
—
—

LAB785
77998.1
—
—
—
1.232
0.10
31
0.649
0.16
17

LAB728
75147.1
—
—
—
1.205
0.17
28
—
—
—

LAB728
75150.2
—
—
—
1.723
L
83
0.673
0.09
21

LAB728
75151.2
—
—
—
1.471
0.03
56
0.678
0.10
22

LAB728
75152.2
—
—
—
1.241
0.10
32
—
—
—

LAB692
76418.1
0.048
0.14
25
—
—
—
—
—
—

LAB692
76418.4
—
—
—
—
—
—
0.625
0.28
13

LAB692
78068.2
0.050
0.08
29
1.742
L
85
0.744
L
34

LAB692
78069.7
—
—
—
1.413
0.03
50
0.668
0.15
21

LAB676
77271.2
—
—
—
1.174
0.21
24
0.666
0.09
20

LAB676
77275.1
—
—
—
1.250
0.12
33
0.641
0.20
16

LAB676
77275.3
—
—
—
1.284
0.05
36
0.663
0.11
20

LAB672
77698.1
—
—
—
1.487
L
58
0.671
0.08
21

LAB672
77698.3
—
—
—
1.485
L
57
0.647
0.16
17

LAB672
77699.1
0.050
0.10
28
1.249
0.09
32
0.679
0.07
22

LAB672
77699.2
0.054
0.03
39
1.343
0.03
42
0.694
0.03
25

LAB672
77700.1
0.052
0.06
34
1.312
0.08
39
0.656
0.14
18

LAB662
77296.3
0.053
0.04
36
1.438
L
53
0.705
0.02
27

LAB662
77298.3
—
—
—
1.418
0.01
50
0.641
0.18
16

LAB662
77299.2
—
—
—
1.623
L
72
0.666
0.10
20

LAB662
77299.4
—
—
—
—
—
—
0.639
0.21
15

LAB633
74828.2
0.048
0.16
25
1.892
L
101
0.716
0.02
29

LAB633
74828.4
0.051
0.07
31
1.261
0.08
34
0.639
0.20
15

LAB633
74831.4
—
—
—
1.408
0.03
49
0.689
0.07
24

LAB633
74832.1
—
—
—
1.229
0.16
30
—
—
—

LAB633
74833.2
—
—
—
1.155
0.22
23
—
—
—

LAB622
75036.1
—
—
—
1.227
0.11
30
0.687
0.05
24

LAB622
75037.3
—
—
—
—
—
—
0.635
0.23
15

CONT.
—
0.039
—
—
0.943
—
—
0.554
—
—

LAB820
76121.1
0.048
0.21
12
1.729
0.11
23
—
—
—

LAB820
76121.2
—
—
—
1.601
0.19
14
—
—
—

LAB820
76122.2
0.052
0.02
21
2.035
L
44
—
—
—

LAB781
75334.1
0.051
0.02
19
1.723
0.17
22
0.722
0.27
9

LAB755
75171.2
0.047
0.27
10
—
—
—
—
—
—

LAB750
75447.2
0.048
0.08
13
1.764
0.15
25
0.726
0.25
9

LAB725
75859.1
0.049
0.06
15
—
—
—
—
—
—

LAB657
75732.2
0.046
0.28
7
1.764
0.05
25
—
—
—

LAB638
76092.1
—
—
—
1.873
0.01
33
—
—
—

LAB638
76092.2
0.047
0.26
11
—
—
—
—
—
—

LAB617
76336.1
0.048
0.26
11
—
—
—
—
—
—

CONT.
—
0.043
—
—
1.409
—
—
0.663
—
—

LAB817
75756.5
—
—
—
—
—
—
0.632
0.24
13

LAB810
76287.1
0.044
0.21
20
1.483
0.07
42
0.678
0.08
22

LAB799
77468.3
0.041
0.26
14
1.436
0.10
37
0.650
0.17
17

LAB799
77470.3
—
—
—
1.365
0.23
31
—
—
—

LAB738
75163.2
—
—
—
—
—
—
0.643
0.18
15

LAB714
75137.2
0.042
0.22
17
1.496
0.03
43
0.647
0.17
16

LAB709
75427.8
—
—
—
1.318
0.22
26
0.645
0.19
16

LAB701
75123.4
0.043
0.15
20
1.630
0.03
56
0.653
0.16
17

LAB701
75124.1
—
—
—
1.629
0.03
56
—
—
—

LAB684
75274.2
—
—
—
—
—
—
0.671
0.12
20

LAB661
75272.1
—
—
—
1.598
0.02
53
0.637
0.23
14

LAB654
76742.3
—
—
—
1.362
0.17
30
—
—
—

LAB631
74856.6
—
—
—
1.318
0.22
26
—
—
—

LAB628
75496.3
—
—
—
1.386
0.18
33
—
—
—

LAB628
75496.5
—
—
—
1.309
0.23
25
0.628
0.29
12

LAB628
75499.1
0.043
0.12
20
1.536
0.03
47
0.661
0.11
19

CONT.
—
0.036
—
—
1.045
—
—
0.558
—
—

LAB850
77714.2
—
—
—
1.703
0.14
29
—
—
—

LAB850
77714.3
—
—
—
1.758
0.09
33
—
—
—

LAB825
76392.1
—
—
—
1.838
0.05
39
—
—
—

LAB825
76394.1
—
—
—
1.639
0.17
24
—
—
—

LAB793
75753.1
—
—
—
1.617
0.21
22
—
—
—

LAB765
76113.3
—
—
—
1.769
0.05
34
—
—
—

LAB765
76113.5
—
—
—
1.980
0.03
50
—
—
—

LAB765
76114.3
0.051
0.08
26
1.767
0.06
34
—
—
—

LAB758
76490.4
0.048
0.27
17
1.876
0.02
42
—
—
—

LAB747
76796.1
—
—
—
1.746
0.13
32
—
—
—

LAB747
76799.1
0.048
0.28
19
—
—
—
—
—
—

LAB735
77323.1
—
—
—
1.860
0.05
41
—
—
—

LAB723
77282.2
—
—
—
1.848
0.05
40
—
—
—

LAB723
77282.3
—
—
—
1.681
0.17
27
—
—
—

LAB711
77077.3
—
—
—
1.900
0.06
44
—
—
—

LAB711
77079.4
—
—
—
1.852
0.03
40
—
—
—

LAB710
76362.2
—
—
—
1.614
0.24
22
—
—
—

LAB710
76365.1
0.049
0.21
21
1.781
0.07
35
—
—
—

LAB698
76204.1
—
—
—
1.692
0.27
28
—
—
—

LAB663
77046.1
—
—
—
1.696
0.19
28
—
—
—

LAB663
77048.1
—
—
—
1.704
0.10
29
—
—
—

LAB656
77316.1
—
—
—
—
—
—
0.751
0.28
13

LAB656
77320.5
—
—
—
1.711
0.15
30
—
—
—

LAB625
76452.1
—
—
—
1.785
0.12
35
—
—
—

LAB625
76472.1
—
—
—
1.647
0.30
25
—
—
—

CONT.
—
0.040
—
—
1.320
—
—
0.667
—
—

“CONT.”—Control

“Ave.”—Average;

“% Incr.” = % increment;

“p-val.”—p-value,

L—p < 0.01.

TABLE 183

Genes showing improved plant performance

at Low Nitrogen growth conditions under

regulation of At6669 promoter (T1 generation)

RGR Of Leaf Area
RGR Of Roots

(cm²/day)
Coverage (cm²/day)

Gene Name
Ave.
P-Val.
% Incr.
Ave.
P-Val.
% Incr.

LAB745
0.044
0.13
16
1.841
0.10
34

LAB734
0.043
0.26
13
—
—
—

LAB685
0.048
0.03
26
—
—
—

CONT.
0.038
—
—
1.373
—
—

“CONT.”—Control;

“Ave.”—Average;

“% Incr.” = % increment;

“p-val.”—p-value,

L—p < 0.01.

These results demonstrate that the polynucleotides of the invention are capable of improving yield and additional valuable important agricultural traits such as increase of biomass, abiotic stress tolerance, nitrogen use efficiency, yield, vigor, fiber yield and/or quality. Thus, transformed plants showing improved fresh and dry weight demonstrate the gene capacity to improve biomass a key trait of crops for forage and plant productivity; transformed plants showing improvement of seed yield demonstrate the genes capacity to improve plant productivity; transformed plants showing improvement of plot coverage and rosette diameter demonstrate the genes capacity to improve plant drought resistance as they reduce the loss of soil water by simple evaporation and reduce the competition with weeds; hence reduce the need to use herbicides to control weeds. Transformed plants showing improvement of relative growth rate of various organs (leaf and root) demonstrate the gene capacity to promote plant growth and hence shortening the needed growth period and/or alternatively improving the utilization of available nutrients and water leading to increase of land productivity; Transformed plants showing improvement of organ number as demonstrated by the leaf number parameter exhibit a potential to improve biomass yield important for forage crops and improve the plant productivity; Transformed plants showing increased root length and coverage demonstrate the gene capacity to improve drought resistance and better utilization of fertilizers as the roots can reach larger soil volume; Transformed plants showing improvement of leaf petiole relative area and leaf blade area demonstrate the genes capacity to cope with limited light intensities results from increasing the plant population densities and hence improve land productivity.

Although the invention has been described in conjunction with specific embodiments thereof, it is evident that many alternatives, modifications and variations will be apparent to those skilled in the art. Accordingly, it is intended to embrace all such alternatives, modifications and variations that fall within the spirit and broad scope of the appended claims.

All publications, patents and patent applications mentioned in this specification are herein incorporated in their entirety by reference into the specification, to the same extent as if each individual publication, patent or patent application was specifically and individually indicated to be incorporated herein by reference. In addition, citation or identification of any reference in this application shall not be construed as an admission that such reference is available as prior art to the present invention. To the extent that section headings are used, they should not be construed as necessarily limiting.

	Number	Date	Country
	61754587	Jan 2013	US
	61693392	Aug 2012	US

ISOLATED POLYNUCLEOTIDES, POLYPEPTIDES AND METHODS OF USING SAME FOR INCREASING ABIOTIC STRESS TOLERANCE, BIOMASS AND YIELD OF PLANTS

Information

Publication Number

Date Filed

Date Published

Inventors

Original Assignees

CPC

International Classifications

Abstract

Description

Claims

PCT Information

Provisional Applications (2)