Claims
- 1. An isolated yeast promoter, which is native to Schwanniomyces castellii (ATCC 26077) and located upstream of and in control of a glucoamylase gene.
- 2. The isolated yeast promoter of claim 1, wherein said promoter has a sequence of 1662 base pairs prior to the initiation codon of the glucoamylase gene.
- 3. A vector comprising the yeast promoter of claim 1.
- 4. The vector of claim 3, wherein said vector is a plasmid vector.
- 5. The vector of claim 4, wherein said plasmid vector is a chromosomal integration vector.
- 6. A method of expressing a gene of interest in bacterial, yeast, mold, and plant/plant cell species, comprising the steps of:
a. fusing a gene of interest to an isolated yeast promoter which is native to Schwanniomyces castellii (ATCC 26077) and located upstream and in control of a glucoamylase gene, and b. integrating the said promoter and the gene of interest within the genomic DNA of a bacterial, yeast, mold, and plant/plant cell, such that the said promoter regulates the expression of a gene of interest in bacterial, yeast, mold, and plant/plant cells.
- 7. A method of expressing a gene of interest in bacterial, yeast, mold, and plant/plant cell species, comprising replicating a vector comprising an isolated yeast promoter which is native to Schwanniomyces castellii (ATCC 26077) and located upstream of and in control of a glucoamylase gene and a gene of interest within a bacterial, yeast, mold, and plant/plant cell genomic DNA such that the said promoter regulates the expression of the gene of interest in bacterial, yeast, mold, and plant tissue/plant cells.
Parent Case Info
[0001] This application is a continuation-in-part of application Ser. No. 09/632,314 filed Aug. 4, 2000.
Government Interests
[0002] The U.S. Government has a paid-up license in this invention and the right in limited circumstances to require the patent owner to license others on reasonable terms as provided for by the terms of Contract DE-AC0676RLO1830 awarded by the U.S. Department of Energy.
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
09632314 |
Aug 2000 |
US |
Child |
09921944 |
Aug 2001 |
US |