Claims
- 1. A composition comprising a substantially pure exo α-N-Acetylgalactosaminidase endogenous to Chryseobacterium.
- 2. A composition according to claim 1, wherein said α-N-Acetylgalactosaminidase is substantially free of contaminating proteases, endoglycosidases and other exoglycosidases.
- 3. A composition according to claim 1, wherein Chyrseobacterium further comprises Chyrseobacterium meningosepticum (ATCC No. 13253).
- 4. A composition according to claim 1, wherein said α-N-Acetylgalactosaminidase is isolated by:
(a) obtaining a Chryseobacterium preparation; (b) incubating the preparation of step (a) with a set of labelled oligosaccharide substrates or monosaccharide chromophores to detect exo α-N-Acetylgalactosaminidase activities in the presence of other glycosidase activities; and (c) isolating and substantially purifying at least one exo α-N-Acetylgalactosaminidase detected in step (b).
- 5. A composition according to claim 1, wherein said exo α-N-Acetylgalactosaminidase is capable of cleaving an αGalNAc 1-R linkage.
- 6. A composition comprising a recombinant exo α-N-Acetylgalactosaminidase from Chryseobacterium.
- 7. A composition according to claim 6, wherein said recombinant exo α-N-Acetylgalactosaminidase is cloned by:
(a) isolating DNA from an organism which produces said exo α-N-Acetylgalactosaminidase; (b) forming a gene library from the DNA of step (a) in a recombinant host organism; and (c) identifying recombinant clones of said recombinant host organism of step (b) having exo α-N-Acetylgalactosaminidase activity.
- 8. A composition according to claim 7, wherein step (c) further comprises the step of utilizing one or more substrates to identify recombinant clones having exo α-N-Acetylgalactosaminidase activity.
- 9. A composition according to claim 6, wherein Chyrseobacterium further comprises Chyrseobacterium meningosepticum (ATCC No. 13253).
- 10. Isolated DNA which codes for an exo α-N-Acetylgalactosaminidase, wherein the isolated DNA is obtainable from Chryseobacterium.
- 11. A recombinant DNA vector comprising a vector into which a DNA segment coding for the exo α-N-Acetylgalactosaminidase has been inserted.
- 12. Isolated DNA coding for the exo α-N-Acetylgalactosaminidase, wherein the isolated DNA is obtainable from Patent Deposit No. ______
- 13. A cloning vector which comprises the isolated DNA of claim 12.
- 14. A host cell transformed by the cloning vector of claims 11 or 13.
- 15. A method of producing a exo α-N-Acetylgalactosaminidase comprising culturing a host cell transformed with the vector of claim 11 or 13 under conditions suitable for expression of said glycosidase.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] The present application is a Continuation-In-Part of U.S. application Ser. No. 08/560,809 filed Nov. 25 1995 which is a Continuation-In-Part of U.S. Ser. No. 08/596,250 filed Jun. 24, 1996, now U.S. Pat. No. 5,770,405, which is a 371 of PCT/US94/10758 filed Sep. 22, 1994 which claims priority of U.S. Ser. No. 08/126,174 filed Sep. 23, 1993, now abandoned.
Divisions (1)
|
Number |
Date |
Country |
Parent |
09428979 |
Oct 1999 |
US |
Child |
09859698 |
May 2001 |
US |
Continuation in Parts (3)
|
Number |
Date |
Country |
Parent |
08560809 |
Nov 1995 |
US |
Child |
09428979 |
Oct 1999 |
US |
Parent |
08596250 |
Jun 1996 |
US |
Child |
08560809 |
Nov 1995 |
US |
Parent |
PCT/US94/10758 |
Sep 1994 |
US |
Child |
08560809 |
Nov 1995 |
US |