ISOLATION/PURIFICATION MAMMALIAN PROSTACYCLIN SYNTHASE

Information

  • Research Project
  • 3508703
  • ApplicationId
    3508703
  • Core Project Number
    R44HL042214
  • Full Project Number
    2R44HL042214-02A1
  • Serial Number
    42214
  • FOA Number
  • Sub Project Id
  • Project Start Date
    9/30/1991 - 33 years ago
  • Project End Date
    9/29/1993 - 31 years ago
  • Program Officer Name
  • Budget Start Date
    9/30/1991 - 33 years ago
  • Budget End Date
    9/29/1992 - 32 years ago
  • Fiscal Year
    1991
  • Support Year
    2
  • Suffix
    A1
  • Award Notice Date
    9/30/1991 - 33 years ago

ISOLATION/PURIFICATION MAMMALIAN PROSTACYCLIN SYNTHASE

Prostacyclin synthase catalyzes an intramolecular redox reaction in which prostaglandin endoperoxide, PGH2, is converted to prostacyclin. The potent biological activities of prostacyclin indicate an important role for prostacyclin synthase in the pathophysiology of thrombosis and cardiovascular diseases. Commercial availability of a purified preparation of mammalian prostacyclin synthase should therefore facilitate basic and pharmaceutical research, including studies of factors involved in the regulation of prostacyclin synthase gene expression, development of novel therapeutic agents for the treatment of cardiovascular diseases, and development of new strategies for the prevention and management of thromboembolic episodes. In Phase II, we will purify prostacyclin synthase from ram seminal vesicles to homogeneity using a variety of methodologies including ion- exchange chromatography, affinity chromatography, isoelectric focusing and gel permeation chromatography. The purified protein will be used to raise both poly- and monoclonal antibodies. The polyclonal antibodies will be used in conjunction with a lambda-gt11 expression library to isolate cDNA sequences specific for prostacyclin synthase. These cDNA sequences will then be used to produce DNA and RNA probes. Attempts will be made to use the monoclonal antibodies developed in a large scale, single step purification procedure. The purified enzyme, the antibodies and the nucleic acid probes will be marketed Oxford Biomedical Research.

IC Name
NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
  • Activity
    R44
  • Administering IC
    HL
  • Application Type
    2
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
  • Sub Project Total Cost
  • ARRA Funded
  • CFDA Code
    839
  • Ed Inst. Type
  • Funding ICs
  • Funding Mechanism
  • Study Section
    SSS
  • Study Section Name
  • Organization Name
    OXFORD BIOMEDICAL RESEARCH, INC.
  • Organization Department
  • Organization DUNS
  • Organization City
    ROCHESTER HILLS
  • Organization State
    MI
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    48309
  • Organization District
    UNITED STATES