Claims
- 1. In a method for recovering an extracellularly produced enzyme from a fungus in a fermentation medium, wherein the improvement comprises killing the fungal cells, without lysing, by a method comprising the following steps in either order:
- (a) adjusting the pH of the medium to a value equal to or less than 2.79 with a mineral acid;
- (b) adding from about 0.25 to about 10% by weight of acetic acid or a salt thereof to the medium
- to thereby kill the cells in the medium under conditions which are compatible with the extracellularly produced enzyme.
- 2. In a method for recovering an extracellulary produced enzyme from Aspergillus niger in a fermentation medium, wherein the improvement comprises killing the Aspergillus niger cells, without lysing, by a method comprising the following steps in either order:
- (a) adjusting the pH of the medium to a value equal to or less than 2.79 with a mineral acid;
- (b) adding from about 0.25 to about 10% by weight of acetic acid or a salt thereof to the medium
- to thereby kill the cells in the medium under conditions which are compatible with the extracellularly produced enzyme.
- 3. In a method for recovering an extracellularly produced enzyme from Saccharomyces cerevisiae in a fermentation medium, wherein the improvement comprises killing the Saccharomyces cerevisiae cells, without lysing, by a method comprising the following steps in either order:
- (a) adjusting the pH of the medium to a value equal to or less than 2.79 with a mineral acid;
- (b) adding from about 0.25 to about 10% by weight of acetic acid or a salt thereof to the medium
- to thereby kill the cells in the medium under conditions which are compatible with the extracellularly produced enzyme.
- 4. The method according to claims 1, 2, or 3 wherein the mineral acid is sulfuric acid or hydrochloric acid.
Parent Case Info
This application is a continuation of application Ser. No. 07/799,864, filed Nov. 27, 1991 now abandoned, which is a continuation of application Ser. No. 07/365,945, filed Jun. 13, 1989 now abandoned.
US Referenced Citations (16)
Foreign Referenced Citations (1)
Number |
Date |
Country |
0148709 |
Jul 1985 |
EPX |
Non-Patent Literature Citations (2)
Entry |
Rusul, G. et al., "Growth and Aflotoxin Production by Aspergillus parasiticis NRRL2999 in The Presence of Acetic or Propionic Acid and at Different Initial pH Values," J. Food Protection, 1987 vol. 50, No. 11, pp. 900 914 919. |
Hentges D. J., "Influence of pH on the Inhibitory Activity of Formic and Acetic Acids for Shigella" J. Bacteriology, vol. 93, No. 6, pp. 2029-2030, 1967. |
Continuations (2)
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Number |
Date |
Country |
Parent |
799864 |
Nov 1991 |
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Parent |
365945 |
Jun 1989 |
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