Claims
- 1. A process of resolving a mixture of 5R and 5S pyridazinone esters of the following formula (A): ##STR12## wherein: R.sup.1 represents lower alkyl;
- R.sup.2 represents hydrogen, halogen, trifluoromethyl, cyano, lower alkyl or loweralkyloxy; and
- R represents alkyl,
- which comprises reacting said ester mixture with a hydrolytic lipase enzyme and recovering i) a mixture of esters of formula (A) optically enriched of formula (A) in one of the 5R or 5S enantiomers and ii) a mixture of the phenols of the following formula (B) optically enriched in the other of the 5R or 5S enantiomers: ##STR13##
- 2. The process of claim 1, wherein said mixture of esters (A) is enriched in the 5S ester and said mixture of phenols (B) is enriched in the 5R phenol.
- 3. The process of claim 1, wherein said reaction is conducted to about 40 to 60% completion.
- 4. The process of claim 1, wherein said reaction is conducted in about a ratio of about 100 to 10,000 units of enzyme per millimole of ester mixture (A).
- 5. The process of claim 1, wherein said reaction is conducted at a temperature of about 0.degree. to 40.degree. C.
- 6. The process of claim 1, wherein said reaction is conducted in an aqueous solution with an organic co-solvent.
- 7. The process of claim 6, wherein said co-solvent is an ether, an alkanol, a ketone or a halogenated hydrocarbon.
- 8. The process of claim 1, wherein said hydrolytic enzyme is lipase derived from Pseudomonas.
- 9. The process of claim 1, wherein R is alkyl of less than 20 carbons.
- 10. The process of claim 1, wherein R is alkyl of less than 4 carbons.
- 11. The process of claim 1, wherein R.sup.2 is hydrogen.
- 12. The process of claim 1, wherein R.sup.1 is methyl.
- 13. The process of claim 1, wherein said mixture (A) is derived from a mixture of approximately the same optical purity of phenols of the formula (B).
- 14. The process of claim 1, wherein said phenol mixture of formula (B) recovered in said reaction is further optically purified by esterification with a source of --C(O)R and reacted again with a hydrolytic enzyme to yield a mixture of phenols (B) having a greater optical purity.
- 15. The process of claim 1, wherein said ester mixture of formula (A) recovered in said reaction is racemized by chemical hydrolysis to recover the corresponding phenol mixture of formula (B) followed by acid catalyzed epimerization at C-5.
Parent Case Info
This application is a continuation-in-part of Ser. No. 402,179, filed 9/1/89, now abandoned.
Non-Patent Literature Citations (2)
Entry |
Kalaritis, Panos et al., Kinetic Resolution of 2-Substituted Esters Catalyzed by a Lipase Ex., J. Org. Chem. 1990, 55, 812-815. |
Whitesides, George M., Enzymes as Catalysts in Synthetic Organic Chemistry, vol. 24, No. 8, Aug. 1985, 617-718. |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
402179 |
Sep 1989 |
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