Claims
- 1. An isolated nucleic acid sequence encoding a microtubule motor protein, wherein the motor protein has the following properties: (i) the protein's activity includes microtubule depolymerization activity; and (ii) the protein has a sequence that has greater than 90% amino acid sequence identity to SEQ ID NO:2 as measured using a sequence comparison algorithm.
- 2. An isolated nucleic acid sequence of claim 1, wherein the encoded protein specifically binds to polyclonal antibodies to a protein comprising SEQ ID NO:2.
- 3. An isolated nucleic acid sequence of claim 1, wherein the nucleic acid encodes SEQ ID NO:2.
- 4. An isolated nucleic acid sequence of claim 1, wherein the nucleic acid comprises nucleotides 840-1805 of SEQ ID NO:1.
- 5. An isolated nucleic acid sequence of claim 1, wherein the nucleic acid selectively hybridizes to a nucleic acid molecule of SEQ ID NO:1 in 50% (v/v) formamide, 0.1% bovine serum albumin, 0.1% Ficoll, 0.1% polyvinylpyrrolidone, 50 mM sodium phosphate buffer pH 6.5, 750 mM sodium chloride and 75 mM sodium citrate at 42° C.
- 6. An expression vector comprising a nucleic acid encoding a microtubule motor protein, wherein the motor protein has the following properties: (i) the protein's activity includes microtubule depolymerization activity; and (ii) the protein has a sequence that has greater than 90% amino acid sequence identity to SEQ ID NO:2 as measured using a sequence comparison algorithm.
- 7. A host cell transfected with the vector of claim 6.
- 8. The nucleic acid of claim 1, wherein the microtubule motor protein consists of amino acids 183 to 546 of SEQ ID NO:2.
- 9. The nucleic acid of claim 1, wherein the microtubule motor protein consists of amino acids 195-566 of SEQ ID NO:2.
- 10. The nucleic acid of claim 1, wherein the microtubule motor protein consists of amino acids 213-546 of SEQ ID NO:2.
- 11. The nucleic acid of claim 1, wherein the microtubule motor protein consists of amino acids 213-566 of SEQ ID NO:2.
- 12. An isolated nucleic acid sequence encoding a microtubule motor protein, wherein the motor protein has the following properties: (i) the protein's activity includes microtubule depolymerization activity; and (ii) the protein has a sequence that has greater than 90% amino acid sequence identity to SEQ ID NO:4 as measured using a sequence comparison algorithm.
CROSS-REFERENCE TO RELATED APPLICATION
The present application is a division of 09/967,908 filed Sep. 28, 2001, now U. S. Pat. No. 6,514,738 continuation-in-part of U.S. Ser. No. 09/675,227, filed Sep. 29, 2000, now U.S. Pat. No. 6,461,855, which is incorporated by reference in its entirety for all purposes.
Foreign Referenced Citations (1)
Number |
Date |
Country |
WO 9953295 |
Oct 1999 |
WO |
Non-Patent Literature Citations (5)
Entry |
Bray-Allen, “Homo Sapiens Chromosome 9 clone RP11-571F15, ***Sequencing in Progress***, 81 unordered places,” GenBank ID No. AL353661, 139 pages. |
Miller et al, “Kinesin homolog (Monodelphis demostica)”, GenBank ID No. AAB62895, 2 pages. |
Miller, et al., “Monodelphis domestica kinesin homolog mRNA, partial CDS,” GenBank ID No. AF005901, 2 pages. |
Neto et al., “Shotgun sequencing of the human Transcription with ORF expressed sequence tags,” PNAS, (97):3491-3496 (2000). |
Wordeman et al, “Identification and Partial Characterization of Mitotic Centromere-associated Kinesin, a Kinesin-related Protein that associates with Centromeres during Mitosis,” J. Cell Biol., 128(1-2):95-105 (1995). |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
09/675227 |
Sep 2000 |
US |
Child |
09/967908 |
|
US |