L5 shuttle phasmids

FIELD OF THE INVENTION
This invention relates to L5 shuttle phasmids which are capable of delivering foreign DNA into mycobacteria. The L5 shuttle phasmids of the invention are used to generate mycobacterial mutations, which can be used to produce mycobacterial vaccines.
BACKGROUND OF THE INVENTION
Tuberculosis (which includes infection caused by M. tuberculosis, M. bovis, BCG and M. africanum) remains the largest cause of human death in the world from a single infectious disease, and is responsible for one in four voidable adult deaths in developing countries. In addition, in 1990, there was a 10% increase in the incidence of tuberculosis in the United States.
In the past, infection with drug-sensitive strains of the M. tuberculosis complex had been cured with certain antibiotics, including isoniazid, rifampicin, ethionamide and pyrazinamide. However, resistance to isoniazid and other antibiotic drugs has developed in many strains of M. tuberculosis. This has resulted in the search for an effective vaccine against M. tuberculosis. Further, this has enhanced the need to develop new drugs which are effective against drug-resistant strains of M. tuberculosis. It is therefore desirable to develop molecular and genetic tools which can be utilized to understand the pathways involved in invasion, survival and persistance of M. tuberculosis and in the development of vaccines and new drugs.
The creation of mutants in M. tuberculosis and BCG is of essential importance in the analysis of M. tuberculosis and BCG gene function. Auxotrophic mutants have been isolated in M. smegmatis by both shuttle mutagenesis and N-methylN'-nitroso-N-nitrosoguanidine treatment followed by isoniazid enrichment. These methods, however, are less effective in the M. tuberculosis complex (M. tuberculosis, M. bovis, M. miroti and M. africanum) due to current difficulties in performing homologous recombination, which is required by the shuttle mutagenesis procedure. Also, the tendency of mycobacteria to clump limits the use of traditional mutagens and makes positive selection advantageous.
Because the creation of mutants in M. tuberculosis and BCG is of essential importance in the analysis of gene function, it is desirable to develop effective means and methods for delivering foreign DNA into M. tuberculosis and BCG. The insertion of foreign DNA into M. tuberculosis and BCG mycobacteria would provide the necessary tools for understanding the mechanisms by which these mycobacteria survive and replicate. In addition, it would provide valuable tools for the development of vaccines and new drugs effective in the treatment of infection caused by M. tuberculosis and BCG.
It is therefore and object of this invention to provide shuttle phasmids capable of delivering foreign DNA into mycobacteria.
It is another object of this invention to provide a method of producing shuttle phasmids capable of delivering foreign DNA into mycobacteria.
It is a further object of this invention to provide a method of generating mycobacterial mutations.
It is another object of this invention to provide mycobacterial mutants.
It is a still further object of this invention to provide a method of producing a mycobacterial vaccine.
SUMMARY OF THE INVENTION
This invention is directed to L5 shuttle phasmids capable of delivering foreign DNA into mycobacteria, which shuttle phasmids comprise an L5 mycobacteriophage containing an E. coli bacteriophage lambda cosmid inserted into a non-essential region of the L5 mycobacteriophage genome.
This invention is further directed to a method of generating mycobacterial mutations comprising producing a mycobacteriophage L5 shuttle phasmid containing a cosmid and a transposon therein, propagating the L5 shuttle phasmid, and infecting mycobacteria with the propagated L5 shuttle phasmids so as to cause delivery of the transposons from the L5 shuttle phasmids to the chromosomes of the mycobacteria, thereby causing mutations in the genes of the mycobacteria to occur. Once mycobacterial mutations have occurred, a marker gene present in the transposons is selected for in order to identify mycobacterial mutants in which the transposons have been delivered, and the mutants can be screened to identify a mutant of interest, such as an avirulent mutant. Certain mutants, including avirulent mutants can be used as mycobacterial vaccines.
In addition, this invention is directed to a method of determining whether an L5 gene is essential for L5 propagation. In order to perform this, a mutation of an L5 gene is generated utilizing a cosmid form of an L5 shuttle phasmid and recombinant DNA methodology so as to obtain an L5 shuttle phasmid mutant. The L5 shuttle phasmid mutant is propagated and then transfected in M. smegmatis in order to determine whether plaques have formed, the presence of plaques indicating that the L5 gene is not essential for L5 propagation.

BRIEF DESCRIPTION OF THE DRAWINGS
The above brief description, as well as further objects and features of the present invention, will be more fully understood by reference to the following detailed description of the presently preferred, albeit illustrative, embodiments of the present invention when taken in conjunction with the accompanying drawings wherein:
FIG. 1 represents the double-cos vector pYUB328 derived from super cos vector. pYUB328 incorporates pak I sites blanking the BAMHI and NorI sites into which random fragments can be cloned. Since mycobacterial genomes do not have pacI sites in their chromosomes, the pYUB328 vector allows for the release of the cloned fragment. pYUB328 contains an E. coli origin in an ampicillin-resistant gene and bacteriophage lambda cos sequences;
FIG. 2 represents a schematic diagram of L5 shuttle phasmid construction;
FIG. 3 represents a schematic diagram of the L5 genome highlighting the L5 cohesive (cos) and the integration regions, immunity regions, and DNA polymerase regions;
FIG. 4 represents a schematic diagram of the L5 shuttle phasmid phAE41 in which the cosmid pYUB328 was inserted into the immunity region of mycobacteriophage L5;
FIG. 5 represents the cosmid pYUB216 which contains the firefly luciferase gene fused to the hsp60 promoter of BCG;
FIG. 6 represents the L5 luciferase shuttle phasmid phAE43 in which cosmid pYUB216 was inserted to replace pYUB328 in phAE41;
FIG. 7 represents the generation of light by the designated shuttle phasmids in M. smegmatis following infection with luciferase reporter phages and luciferase reporter shuttle phasmids;
FIG. 8 represents the M. smegmatis insertion element IS1096 and transposons derived from it. The IS element has two major ORFs; ORFA and ORFR (marker by filled arrows), bounded by inverted repeats (IRs). Transposons were constructed by inserting an aph gene (shaded arrow), between the ORFs, into the MluI site (Tn5368), or into a SalI site within ORFR (TN5368); or in an NcoI site within ORFA (Tn5369). Relevant restriction sites are indicated;
FIG. 9 represents plasmid constructs used to assess transposon activity in M. bovis BCG. All plasmids contain an aph gene for kanamycin resistance, which constitutes part of the transposons in pYUB285, pYUB297 and pYUB305. They also have a disabled oriM (represented by a single arrow) which contains a 556 bp deletion, marked by a bar. pYUB312 contains the aph gene outside the IS element, so transformants will only be seen if integration of the plasmid has occurred;
FIG. 10 is comprised of FIGS. 10A, 10B and 10C, and represents Southern blots of six clones resulting from transposition with Tn5367 (1-3) and Tn5368 (4-6). Lane 1, mc.sup.2 826; 2, mc.sup.2 827, 3, mc.sup.3 838; 4, mc.sup.2 829; 5, mc.sup.2 830 and 6, mc.sup.2 831. M, pYUB285; s, pYUB297. DNA was digested with A: KpnI, B: BamHI and C: Pvul. Blots were probed with pYUB285 and mobilities are indicated by markers in kbp;
FIG. 11A and 11B are graphical representations of the sizes of restriction fragments obtained from a total of nineteen BCG clones picked at random. FIG. 11A depicts the restriction fragments of those clones transformed with Tn5367-containing plasmid pYUB285. FIG. 11B depicts the restriction fragments of those clones transformed with Tn5368-containing plasmid pYUB297. The clones were digested with KpnI (black bars) or BamHI (hatched bars) and hybridized with pYUB285. They have been arranged in descending order according to the KpnI fragment size to show the randomness of transposition and allow comparison of clones having similar KpnI fragments but different restriction patterns with BamHI;
FIG. 12 represents a DNA sequence found on either side of the transposon in the six clones examined. An eight base-pair direct repeat is present in each clone, presumably from duplication of target DNA. mc.sup.2 826, mc.sup.2 827 and mc.sup.828 are clones containing Tn5367 and mc.sup.2 829, mc.sup.2 830 and mc.sup.2 831, contain Tn5368;
FIG. 13 represents the survival of transposon-derived auxotrophs of BCG in mice. Methionine auxotroph grows in a fashion similar to wild-type BCG as compared to the leucine auxotrophs, which are quickly lost from both the mouse spleen and lungs;
FIG. 14 represents a cosmid pYUB435 containing the IS1096 derived transposon TN5367; and
FIG. 15 represents a transposon delivery shuttle phasmid phAE46 derived from phAE41 containing the cosmid pYUB435.

DETAILED DESCRIPTION OF THE INVENTION
This invention is directed to L5 shuttle phasmids capable of delivering foreign DNA into mycobacteria. The L5 shuttle phasmids are produced by inserting a cosmid into a non-essential region of an L5 mycobacteriophage genome. In addition, other foreign DNA can be inserted into the L5 shuttle phasmid, including reporter genes (such as a luciferase gene), a transposon (such as IS1096), and a gene which encodes a mycobacterial inhibitor (such as anti-sense RNA which has a target of a mycobacterial gene necessary for mycobacterial survival). Further, a DNA-modifying enzyme, and RNA-modifying enzyme or a protein-modifying enzyme can be inserted into the L5 shuttle phasmids of the invention for subsequent insertion into mycobacteria, including M. tuberculosis, M. smeqmatis, BCG and M. bovis.
Once L5 shuttle phasmids are obtained, they can be utilized to generate mycobacterial mutations. These mutations can be used to study the mechanisms of mycobacteria as well as to develop vaccines and drugs effective in the treatment of mycobacteria.
In order to generate mycobacterial mutations, L5 shuttle phasmids are produced by inserting a cosmid, such as an E. coli bacteriophage lambda cosmid, and a transposon, such as IS1096, into the genome of an L5 mycobacteriophage. Once the cosmid and transposon are inserted into the L5 mycobacteriophage, an L5 shuttle phasmid is created and is then propagated in a conditional host. The propagated L5 shuttle phasmids are infected into mycobacteria so as to cause delivery of the transposons from the L5 shuttle phasmids to the chromosomes of the mycobacteria, thereby causing mutations in the genes of the mycobacteria to occur. A mycobacterial mutation library is thereby obtained.
In order to produce mycobacterial vaccines, the mycobacterial mutation library is utilized. The presence of a marker gene in the transposons is selected for. This identifies mycobacterial mutants in which the transposons have been delivered into the mycobacteria. Screening is then performed in order to identify a mutation of interest. For example, screening can be performed for an avirulent mutant. Avirulent mutants and other mutants can then be used as mycobacteiral vaccines. Examples of marker genes which can be selected for are kanamycin resistance genes, hydromycin resistance genes and L5 immunity genes. An example of an avirulent mutant which can be screened for is a leucine auxotroph.
Further, this invention is directed to a method of determining whether an L5 mycobacteriophage gene is essential for L5 propagation. In order to perform this, a mutation of an L5 gene is generated utilizing a cosmid form of an L5 shuttle phasmid and recombinant DNA technology so as to obtain an L5 shuttle phasmid mutant. The mutant is then propagated and transfected into M. smegmatis in order to determine whether plaques have formed. If plaques have formed, this indicates that the L5 gene is not essential for L5 propagation. If no plaques have formed, this indicates that the L5 gene is essential for L5 propagation. The construction of several L5 shuttle phasmids of the invention is described below.
EXAMPLE 1
Construction of L5 Shuttle Phasmids
In order to construct an L5 shuttle phasmid, an E. coli cosmid pYUB328 (see FIG. 1) was inserted into a non-essential region of the L5 mycobacteriophage genome (see FIG. 3) by the method described by. Jacobs, et al., Nature, Vol. 327, pp.532-536 (1987). Cosmid pYUB328 was deposited with the American Type Culture Collection, Rockville, Md., on May 20, 1994 and catalogued as ATCC No. 69631. FIG. 2 represents a schematic diagram of L5 shuttle phasmid construction in accordance with the present invention. This generated a cosmid library of partially-digested mycobacteriophage genomes which replicated in E. coli as recombinant plasmids. Individual mycobacteriophage-cosmid clones were analyzed and it was determined that construction of the library had generated a set of cosmid-insertions at random sites around the L5 mycobacteriophage genome, which is typically accompanied by the generation of a small deletion of the mycobacteriophage genome at the site of insertion. This library was transfected into M. smegmatis cells and resulted in the identification of recombinant mycobacteriophages which have the E. coli cosmid inserted at non-essential regions of the L5 mycobacteriophage.
Previously constructed shuttle phasmids, for example the L1 shuttle phasmid constructed by Snapper et al., Proc. Natl. Acad. Sci. USA, Vol. 85, pp. 6987-6991 (1988), have not been as useful for insertion of foreign DNA into mycobacteria as they have contained deletions of the cosmid. The inventors concluded that these deletions likely indicate that mycobacteriophage L5 has rigorous packaging constraints, and therefore used a smaller, double-cos, cosmid, pYUB328, which as constructed by Balasubramanian, et al., (1994). This yielded a 3.8 kb cosmid following in vitro packaging into lambda heads.
A library of over 5000 Ap.sup.r (ampicillin-resistant) pYUB328::L5 recombinant clones was generated. Cosmid DNA isolated from E. coli was transfected into M. smegmatis in order to propagate it. Transfection of plasmid DNA of the library isolated from E. coli into M. smegmatis cells yielded 34 plaques. Eleven of these plagues were found to hybridize to pYUB328. DNA was prepared from mycobacteriophage particles and analyzed by restriction analysis. Five different classes of pYUB328 insertions into L5 were generated, which represented different size deletions of the genome. All of the L5 shuttle phasmids contained pYUB328 inserted near the immunity gene of mycobacteriophage L5. One of the shuttle phasmids was designated phAE41 by the inventors (see FIG. 4). L5 shuttle phasmid phAE41 was deposited with the American Type Culture Collection, Rockville, Md. on May 20, 1994, and catalogued as ATCC No. 69624.
EXAMPLE 2
Molecular Characterization of L5 Shuttle Phasmid phAE41
Experiments were performed to confirm that phAE41 was a shuttle phasmid constructed from mycobacteriophage L5. DNA isolated from mycobacteriophage particles of phAE41 was ligated together to yield long concatamers and then packaged in vitro into bacteriophage lambda heads. The resulting bacteriophage-packaged particles were capable of transducing ampicillin-resistance at high frequencies. This was determined by mixing the in vitro packaged lysate with a-sensitive E. coli cells and then plating on agar containing 5 .mu.g/ml ampicillin. The phAE41 yielded 10.sup.4 ampicillin-resistant colonies. Comparisons of restriction analyses of plasmids isolated from E. coli and phage-digested molecules demonstrated identical patterns except for the unligated cohesive ends of the linear phage molecules. These results demonstrated that the phAE41 molecules were stable in both mycobacteria and E. coli. However, unlike the phAE15 shuttle phasmid derived from L1 (Snapper, et al., 1987), the entire cosmid in the L5 shuttle phasmid was stably maintained in shuttle phasmid phAE41. In addition, the E. coli cosmid portion of the shuttle phasmid could be completely removed from the phAE41 with any of three enzymes, PacI, NotI or EcoR1. Restriction analyses was used to determine the site of insertion of the pYUB328 cosmid, and thus the complete sequence of the phAE41 was deduced. The map of phAE41 is shown in FIG. 4. The sequence for phAE41 is shown below:
SEQ ID NO: 1__________________________________________________________________________ 1 GGCGCTCTCG CATCGCATCG AGTGTTTGCT GTGTCTCTCA TCGTCGCAGG TCAGAAGGGG - 61 TAGGGGGGTT CCCCCTAGGG GTCGGTCCTT GACCGGTCGG TTAGGTCGGT TATGCGGCC - 121 AGCCATCCTG TACGGGTTTC CAAGTCGATC AGAGGTAGGG GCCGGCACAG AAACCACTCA - 181 CATCAGGGCT GTGCGCCTCC AGGGCGCGTG AACTCCCACA CCCCGGTGTA GTTACATCCC - 241 GGAATTGTCT CAGCGCCTCT CAGGGCGCTT CTCATAAACA GTGATCTACG CCACTCCTGA - 301 CGGGTGGCTG TCAAGGATAC TCACCTTCCC TACTAATGAG GGGCTAAGAG CCCCTCTCTA - 361 TAGAGCGCCG CACAGGCGGC GCGATAAGAG CGCCACCAGG CGCTCATCTA AAGACCGGCC - 421 TTGAAGGGCC GGTCATAGAG ATCTATTCGA TCCGGCAACC GCCGGATCTC AAGGCCGCGC - 481 CAGTGCGCGG CCCTATAGAG GGGTGACTCA ACTGTGCATG GCACTCGCTC GAGTGCCCAC - 541 TGGAGCACTC AACCGGGGAA GTTCGACGTT CTCAACCTGC GAATGACGTT TGAATCGTCA - 601 TCCGCGTACG AAATCCCCGA TCTGCGGCCG ACCGACTTCG TGCCGGCCTA TCTCGCGGCC - 661 TGGAATATGC CGCGTCACCG CGATTACGCC GCCAAGAACG GCGGCGCGCT GCACTTCTTC - 721 CTTGACGATT ACCGGTTTGA GACCGCGTGG TCGTCCCCCG AGCGCCTTCT CGACCGCGTA - 781 AAGCAGGTCG GCGCTGCACT CACGCCGGAT TTCAGCCTCT GGACGAACAT GCCGAAGGCG - 841 GCGCAGCTAT GGAACGTCTA CCGCTCCCGC TGGTGTGGCG CGTATTGGCA GTCGGAAGGA - 901 ATCGAGGTGA TTCCGACGGC GTGTTGGGCG ACTCCCGACA CGTTCGATTT CTGTTTCGAC - 961 GGGATCCCGA TGGGATCGAC CGTCGCAATT TCTTCGATGG GCATTCGCTC TTCAAAAGTC - 1021 GACCAGGAGC TTTTCCGGTA CGGACTACGC GAACTCATCG ATCGCACTCA ACCGCAACTG - 1081 CTTTTGGCAT ATGGCCAGCT TCGGCATTGC GACGACATGG ATTTACCAGA GGTCCGCGAA - 1141 TACCCGACCT ACTGGGACAG ACGACGAAAG TGGGTAACTG CCGATGGGAG GCCGGGGAAG - 1201 TAAAGGCGGC CCCGGTCCCG GAACCGGAGC ACGCAACCGC AGAGGCGCTG GAGCCCCCGG - 1261 ATCGGGCGGC GTAGGCGGCG TCGGAGGCGG GGGTGGAGCT GCAGGGAGCA GCGGAGGCGG - 1321 CAAGGGAACG GCAGCGCCGG TACCGGAGGC GTCACCGGTG GCGGCGGAAG TGGAGCCGGC - 1381 GGCGGTGGCA GCAGCCCCAA CACCCCGGTG CCCCCCACCG AGCTGGAGAA GAAGCGCGGC - 1441 GAATACAACC AGATCGCCAT CGACGCCCAG AAACAGCACG CGCCCACCGA TGAGAAGCGC - 1501 GAGGCCAAGC GCAAGCAACT GATGGATCGA GTCGGAGGAG ACTGGCAGGC TTTGGACCCG - 1561 GATCACCACG ACGCCATCAA GGTGGCGATG GATGACGCCA TGCGGAAGAT CCTCTCCGAG - 1621 GAGGAGATCG TCCACCGCAC CAAGCACTTC GGCGACCTAC TCGACCATAC TCGACTCAAG - 1681 TCGCTGTTCG AGGTCGGCTT CTCAGCCGGT GGCGACACCC CGACCGAACG CGCCCTCCTC - 1741 GAGGACGCCT GGTTCGGCGC AGGCAAGGTT CCCCCGATCT ACTCGGCAAT CGAGTTCAAC - 1801 GGCGCTCCGA CAGCCGGCCT CGGCATGTAC GGCGGCACCA AGCTCTACAT GAAGGACTCG - 1861 GTCAAGGACC GCGTCACCGT GACCATCGGC GACTCGCTGA TGTCGAGCTG GGACGTATTC - 1921 CCCGGCCGTC CTGGCGACGG CGTGGGGCTG TGGGCCAGCC TGTCGAAGAT CGAGGGGCTG - 1981 GTCGATCCGA GCAAGACCCG CGAAGAGAAC ATGCAGGCGG TGTACGACTC GTTCAAGAAG - 2041 TACGGCACCC TGGACGGCTT CATCGAGGCG CAGATCCACG GCGGCGTCCT GGTCGAGGAC - 2101 ATCAAGAAGG TCGTGTTCAC GCAGCCGCCG AGCCCGATCT TCACCGATAA ACTGGACGAA - 2161 CTTGGAATCC CGTGGGAGGT GCAGTAATGG CGCAGATGCA GGCGACACAC ACAATCGAGG - 2221 GGTTCCTGGC TGTCGAGGTG GCCCCTCGGG CGTTCGTCGC AGAGAACGGC CACGTACTGA - 2281 CCCGGCTGTC GGCCACGAAG TGGGGCGGTG GCGAGGGTCT CGAGATCCTC AACTACGAGG - 2341 GTCCAGGGAC CGTCGAGGTC TCCGACGAGA AGCTCGCCGA AGCCCAGCGG GCCAGCGAGG - 2401 TCGAGGCTGA ACTTCGCCGC GAGGTCGGCA AGGAGTGAGC TGGGCCGGCT CAGGCCGGCG - 2461 ACAGGAACTA CCAGAGGACT GGGAGCTGAA TTACCGGCTC CCGGTCCTTT CTGCTGCCAA - 2521 CTGGCTTTGC CAGATCAACG GTCCCGGATG CGTAAGGGCC GCAACCGATG TCGACCACAT - 2581 CAAGCGCGGG AACGACCACA GCCGGTCCAA TCTGCAGGCA GCCTGCCATG TCTGTCACGG - 2641 CAAGAAATCA GCCGCCGAGG GCGTAGCCCG ACGGCGGGAA CTTAGAGCCC GGAGGAAGCG - 2701 ACCACCCGAA CGCCATCCTG GGCGTCGATA AGCGGGCCAG GTGCCCGCTC CACCCAGGAG - 2761 GTGAACAGTG GGCACGCGAG GCCCAATCGG AAAACGAGAT GAAGAGCGGG TTCGTCGGAA - 2821 CACCCCGGAC AGTCCAACCG ACACGATCCA GATGCCCGGT CTGGTGACGA TCCCCGAGAT - 2881 GGGCGATCTA AGCCACGACG GCCGCACGCA CCAGCTCGTC AAGGACATGT ACGAGTCGAT - 2941 CAAGCAGTCG GCAGCCGTGA AGTACTACGA GCCGACCGAC TGGCAGATGG CCCGACTCGC - 3001 CCTCTACACA CTTAACCAGG AACTCATCGC AGCCGAGAAC AACGGCAAGC CCGTGGGCGC - 3061 GATGAAGCTC ACTGCCATCA ACCAGATGCT CTCCGCGCTG CTGCTGACCG AAGGTGACCG - 3121 ACGCCGCGTC CGACTCGAAG TCGAACGAGC ACCCGCTGAC CCGACAGGCG GGAAGGTCGT - 3181 TGACGTGACC GACGTGCTCA AGCAGCGCCT CGCCAAGGCG AGCGGCGGGA GCTGATGGTC - 3241 CCCCGAGGGG TTTCTAGAGC CGCTGCCGCT ACCAGCCGCT CCCCCTCGGG GTAGACATCG - 3301 AAAGGAACCA CATGGCCGAC CTCGGCAACC CACTCGACCT CGAGATGCTC TGCCTGGTCA - 3361 CAGGCCGGGA CTTCCGCTGG ACCATCGATT ACCCGTGGGG TCCGGGAGAG CTGTTCCTCG - 3421 AACTCGAGAC CGGCGGCGAA CACAACGCGC TGCATCAGGT CTATGTCACC GGGGCGACCG - 3461 GAGGCACGTA CACGCTGAAC GTCAACGGCA CCAACACCCC GGCCATCGAC TACAACGACG - 3541 TGTCGGAGAA TCCGCAGGGG CTGGCAGGCG ACATCCAAGA CGCTCTGGAC GCAGCCGTCG - 3601 GAGCCGGAAA CGCTGTCGTG CATCCGGTCT CGCTGTTCCC TGCGTGGACA CTGAACTTCA - 3661 ACCTCAACGC CAGCAAGCCG CTCACCGAGC AGTTGGTCAA CACGATCAAC AAGGCCGCGA - 3721 ACGACTTCTT CGACACGTTC GACCAACTAC TTGGGGTCGA CGTGGAGATG ACGGTCACCG - 3781 ACACCCTGAA CTTCAAGCTC AAGGTGACCT CGCGGCGCTC GTTCGATGAG GTCGGTGTCG - 3841 TCACGTTCGC GGTCGACGTG ACCAGCCAGG CAGTCATCAA CTTCTTCAAC TCCGTCGCCG - 3901 AACTCACCGG AGCGGTGAAC ACCGTCAACG TCGACTTCTA CTGGAACCGG ACGTATGACA - 3961 TCGAGTTCAC CGGATCCCTT GGGCTGCAGC CGATTCCGGC TACTACAGCC GACATCACCA - 4021 ACCTGGCGGG TACCAGCAAG GCCGTCTCAG TCACGGTGGT CGAGCCAGGA AAGAAGAGGC - 4081 TGACCATCTG GCCGTTCACG GTCAACGGTG AAACCGCAAC CATCAAGGTC GAGTCCGAAG - 4141 AGGCCGACAA GATCCCCAAC CGCTGCCGCT GGCAGTTGGT TCACATGCCG ACCGGCGAGG - 4201 CAGCCGGCGG CGATGCAAAG CAGCTCGGCC GCGTTTACCG ACAGCCGAGG TAACACCGCA - 4261 CCCATCAGAG ATGGTGGGCC AGACGGCCTT CGGGCCGTCC CCTGACGTGT AGCTCAATGG - 4321 CAGAGCGCCC GACTGTTAAT CGGGTGGTTG AAGGTTCGAG TCCTTCCATG TCAGCGAGGG - 4381 CTGAACCGGA CCCGTGTCCG GTGTAGGCAC TTTCCGCAGG CGGTTCCCCA GAGCGTGGGG - 4441 AGCCCCTGCC CTGTACACGT AGCTCAATTG GTAGAGCAGC GGTCTCCAAA GCCGCCGGTT - 4501 CCAGGTTCGA CTCCTGGCGT GTATGCACAC ACCCCTGACT CCTGCTAGCG GAGTGTTCGC - 4561 CTTTCGGGCC TGGGGTCTTT TTCCCCGTTC GTCTAATCGG TAAGACACCC GGCTCTGGAC - 4621 CGGGCAATTG AGGTTCGAGT CCTTGGCGGG GAGCCAACTT GACATCCACC CGAAAGGAAC - 4681 AACATGACCT TCACAGTCAC CCGCGAGAGA GCGCAGTGGG TCCACGACAT GGCCCGCGCT - 4741 CGCGACGGTC TCCCCTACGC GTACGGCGGG GCGTTCACCA ACAACCCGAG GGTGTCGACT - 4801 GACTGCTCTG GCCTGGTGCT GCAGACCGGG GCTTGGTATG GAGGTCGCAC CGACTGGGTC - 4861 GGAAACCGTT ACGGCTCAAC CGAATCGTTC CGGCTCGACC ACAAGATCGT CTACGACCTA - 4921 GGGTTCAAGC GGATGCCCCG AGGCGGGCCA GCGGCCTTGC CGATCAAGCC GGTGATGCTC - 4981 GTCGGGCTCC AGCACGGAGG CGGCGGGGTC TACTCGCACA CCGCTTGCAC GTTGATGACG - 5041 ATGGACCACC CCGGTGGCCC GGTCAAGATG TCCGACCGAG GCGTCGACTG GGAGTCCCAC - 5101 GGCAACCGCA ACGGCGTAGG CGTCGAACTT TACGAGGGCG CACGGGCATG GAACGACCCT - 5161 CTGTTCCATG ACTTTTGGTA CCTGGACGCA GTCCTCGAAG ACGAAGGAGA CGATGACGAA - 5221 TTGGCTGACC CAGTTCTAGG GAAGATGATC CGCGAGATCC ACGCGTGCCT GTTCAATCAG - 5281 ACCGCGTCGA CCAGCGATCT GGCGACCCCT GGTGAAGGCG CTATCTGGCA GCTACACCAG - 5341 AAGATCCACT CGATTGACGG CATGCTCCAC CCGATCCACG CTGAGCGGCG CGCTCGCGCA - 5401 GGCGATCTCG GTGAGCTGCA CCGAATCGTG TTGGCCGCGA AGGGCTTGGG CGTGAAGCGC - 5461 GACGAGGTGA CCAAGCGGGT CTACCAGAGC ATCCTCGCCG ACATCGAGCG GGACAACCCC - 5521 GAAGTACTTC AGCGATACAT CGCAGAAAGA GGTGGCCTAT GAGCCCCAAG ATCCGACAGA - 5581 CCATCTACCT GCTCGGCACC GCCGCCCCGG CACTGCTGGG CATCGTCCTG ATCTGGGGCG - 5641 GGCTCGACGC TGAGTCGGCG GCTGACCTCG GTGACATCAT TGCGGGCGTC GTGTCGATAC - 5701 TAGTCTCCGG TGCGCCGGCC GTAGCGGCAG GCACCGTACG CAGCCAGCGC AAGGACGGCA - 5761 CGTTGTCCAC CAGCCCGGTG GATCAGGTCA CCAAGGGCGT CGAGCAGGTG CTCGCGGCCA - 5821 GGCAGAGTGC CGAGGCTGAA GTCGCGAAGG TCAAGCAGGC GCTGGAGACC GCCGTCAGCG - 5881 GTTCTCTCCC CCAGCTCGGC CCGCTGGCCA CGCAGATCCT CAACGTGGCT GACGACACCG - 5941 TCTGGCGTCC ATGAGCAAGC CCTGGCTGTT CACCGTCCAC GGCACAGGCC AGCCCGACCC - 6001 GCTCGGGCCT GGTCTGCCTG CCGATACCGC ACGGGACGTA CTTGACATCT ACCGGTGGCA - 6061 GCCCATCGGC AACTACCCGG CAGCGGCGTT CCCGATGTGG CCGTCGGTCG AAAAGGGTGT - 6121 CGCTGAGCTG ATCCTGCAGA TCGAGCTGAA GCTGGACGCA GATCCGTACG CGGACTTCGC - 6181 GCTGGCCGGC TACTCGCAGG GAGCCATCGT GGTGGGCCAG GTGCTCAAGC ACCACATCAT - 6241 CAACCCGAGA GGTCGACTGC ACCGGTTCCT GCACCGGCTC AGGAAGGTCA TCTTCTGGGG - 6301 TAATCCGATG CGGCAGAAGG GCTTTGCCCA CACCGACGAG TGGATTCACC AGGTCGCTGC - 6361 CTCGGACACG ATGGGCATCC TCGAGGACCG ACTGGAGAAC CTCGAGCAGT ACGGCTTTGA - 6421 GGTCCGCGAC TACGCGCACG ACGGCGACAT GTACGCCTCC ATCAAGGAGG ACGACATGCA - 6481 CGAGTACGAG GTGGCCATTG GCCGAATCGT GATGAGCGCT AGGCGATTCA TCGGAGGTAA - 6541 GGACTCCGTC ATCGCCCAGC TCATCGAGCT TGGACAGCGT CCGATCTGGG AGGGAATCGC - 6601 GATGGCCAGA GCCATCATCG ACGCCCTCAC GTTCTTCGCC AAGTCGACCC AAGGCCCGAG - 6661 CTGGCCGCAT TTGTACAACC GCTTCCCGGC GGTCGAGTTC CTACGACGAA TCTGAGAAAG - 6721 GAGGCGGGGT GAGCCTCAAC AACCACCACC CGGAGCTTGC CCCGTCTCCC CCTCACATCA - 6781 TCGGCCCGTC CTGGCAGAAG ACGGTCGATG GTGAGTGGTA TCTGCCTGAG AAGACCCTCG - 6841 GCTGGGGAGT CCTGAAGTGG CTCTCCGAGT ACGTGAATAC CCCGGGCGGG CATGACGATC - 6901 CGAACCGTCT GGCGACGTTG ATCGCGCTCT CCGAGGCAGG TCTTCTCGAC AACGAGAACA - 6961 TGTTCATCCC CACCGACGAG CAGGTACGCC TGGTCCTCTG GTGGTACGCA GTAGATGACC - 7021 AGGGCCAGTA CATCTACCGC GAGGGCGTGA TCCGCCGGCT CAAGGGCTGG GGCAAGGATC - 7081 CGTTCACCGC CGCGCTCTGC TTGGCGGAAC TCTGTGGCCC CGTAGCCTTT TCACACTTCG - 7141 ACGCCGACGG TAACCCGGTC GGCAAGCCGC GTTCAGCCGC GTGGATCACC GTCGCGGCCG - 7201 TCAGCCAGGA CCAGACGAAG AACACGTTCT CGCTGTTCCC GGTGATGATC AGCAAGAAGC - 7261 TGAAGGCCGA GTACGGCCTG GACGTGAACC GCTTCATCAT CTACTCCGCA GCCGGTGGCC - 7321 GTATTGAGGC AGCGACCTCG AGCCCCGCGT CGATGGAGGG TAACCGCCCG ACGTTCGTCG - 7381 TCCAGAACGA GACGCAGTGG TGGGGCCAAG GCCCCGACGG CAAGGTCAAT GAAGGCCACG - 7441 CGATGGCAGA GGTCATCGAA GGCAACATGA CCAAGGTCGA GGGCTCCCGC ACCCTGTCGA - 7501 TCTGCAACGC CCACATCCCC GGCACCGAGA CGGTCGCCGA GAAGGCATGG GACGAGTACC - 7561 AGAAGGTCCA GGCAGGCGAC TCTGTCGACA CCGGGATGAT GTACGACGCG CTGGAAGCGC - 7621 CGGCCGACAC CCCGGTCTCC GAGATCCCCC CGCAGAAGGA GGATCCCGAG GGATTCGAGA - 7681 AGGGCATCGA GAAGCTCCGC GAGGGCCTGC TCATCGCCCG AGGCGACTCC ACCTGGCTGC - 7741 CGATAGACGA CATCATCAAG TCGATTCTGT CGACCAAGAA CCCGATCACC GAGTCGCGGC - 7801 GCAAGTTCCT GAATCAGGTA AACGCCGCTG AGGACTCGTG GCTCTCACCG CAGGAATGGA - 7861 ACCGGTGCCA GGTCGACCTG GCCAAGTACC TGGATAAGCA CGGCAGGGAG TTCGCTCCGC - 7921 TGCAGCGCGG TGACCGGATC ACCCTCGGGT TCGACGGGTC GAAGTCCAAC GACTGGACCG - 7981 CGCTCGTCGG CTGCCGTGTC AGCGACGGCC TGCTGTTCGT CATCGACATC TGGGATCCCC - 8041 AGAAGTACGG CGGGGAGGTT CCCCGCGAAG ACGTTGACGC CAAGGTCCAT TCGGCGTTCG - 8101 CCCACTACGA CGTGGTGGCG TTCCGCGCCG ACGTGAAGGA GTTCGAGGCG TACGTCGACC - 8161 AGTGGGGCCG GACCTACAAG AAGAAGCTCA AGGTCAACGC CAGCCCGAAC AACCCGGTGG - 8221 CGTTCGACAT GCGCGGACAG CAGAAGAGGT TCGCGTTCGA CTGCGAGCGA CTCGAGGACG - 8281 CGGTCCTTGA GGGCGAGGTC TGGCACGACG GCAATCCCGT TCTGCGCCAA CACGTTCTGA - 8341 ACGCCAAACG ACACCCAACG AACTACGACG CCATCGCGAT TCGCAAGGTC ACGAAGGACT - 8401 CCAGCAAGAA AATCGACGCT GCAGTCTGCG CTGTCCTCGC GTTCGGGGCG AGACAGGACT - 8461 ACCTCATGAG CAAGAAGGCC CGTAGCGGCC GGGTGGTGAT GGTTCGATGA CAGCACCGCT - 8521 CCCCGGTATG GAGGAGATCG AAGACCCCGC AGTCGTACGA GAAGAGATGA TCTCGGCCTT - 8581 CGAGGATGCT TCCAAGGATC TCGCCAGCAA CACCAGCTAC TACGACGCTG AGCGCCGGCC - 8641 AGAGGCCATC GGCGTCACCG TCCCGAGAGA GATGCAGCAA CTGCTGGCTC ACGTCGGATA - 8701 CCCCAGGCTC TACGTCGACT CAGTCGCCGA GCGCCAGGCC GTCGAGGGTT TCCGCCTCGG - 8761 CGATGCCGAC GAGGCTGACG AAGAGCTGTG GCAGTGGTGG CAGGCCAACA ACCTCGACAT - 8821 CGAGGCACCA CTGGGCTACA CCGACGCTTA CGTTCACGGC CGGTCGTTCA TCACGATCAG - 8881 CAAGCCAGAC CCGCAGCTCG ACCTGGGTTG GGATCAGAAC GTCCCGATCA TCCGCGTCGA - 8941 GCCGCCCACC CGAATGCACG CCGAGATCGA CCCCCGGATC AACCGGGTGT CCAAGGCCAT - 9001 CCGAGTCGCA TATGACAAGG AGGGCAACGA GATTCAGGCT GCCACGCTGT ACACGCCGAT - 9061 GGAGACCATC GGCTGGTTCC GCGCTGACGG TGAGTGGGCT GAGTGGTTCA ACGTCCCGCA - 9121 CGGTCTGGGC GTCGTTCCCG TTGTGCCGCT TCCGAACCGG ACCCGGCTCT CGGACCTGTA - 9181 CGGCACCAGT GAGATCACGC CCGAGCTTCG GTCGATGACC GACGCGGCGG CGCGCATCCT - 9241 CATGTTGATG CAGGCGACCG CCGAGCTGAT GGGTGTCCCC CAGCGCCTGA TCTTCGGCAT - 9301 CAAGCCCGAA GAGATCGGCG TCGACTCCGA GACCGGCCAG ACGCTGTTCG ATGCGTACCT - 9361 GGCCCGGATC CTGGCGTTCG AGGACGCTGA GGGCAAGATC CAGCAGTTCT CTGCAGCCGA - 9421 GCTGGCCAAC TTCACCAACG CGCTCGATCA GATCGCCAAA CAGGTCGCTG CGTACACGGG - 9481 ATTGCCTCCC CAGTACCTGA GTACCGCCGC AGACAATCCG GCCTCCGCTG AGGCGATCAG - 9541 GGCCGCTGAG AGCCGACTCA TCAAGAAGGT CGAGCGGAAG AACCTGATGT TCGGCGGCGC - 9601 ATGGGAAGAG GCCATGCGGA TCGCCTACCG GATCATGAAG GGCGGCGACG TTCCCCCGGA - 9661 CATGCTCCGC ATGGAGACCG TCTGGCGAGA CCCGAGCACT CCCACCTACG CGGCCAAGGC - 9721 CGACGCAGCC ACGAAGCTGT ACGGCAACGG CCAGGGTGTC ATCCCGCGTG AACGTGCTCG - 9781 CATCGACATG GGCTACTCCG TCAAGGAGCG CGAAGAGATG CGCCGATGGG ACGAGGAAGA - 9841 GGCCGCAATG GGTCTCGGCC TGTTGGGCAC GATGGTCGAC GCCGACCCGA CGGTCCCAGG - 9901 CTCCCCGAGC CCCACGGCAC CGCCGAAGCC ACAGCCGGCC ATCGAGTCGT CTGGTGGTGA - 9961 TGCGTGACCG CAGAGGAGTA CGCGGCGGCT CAAGCCGCGA TCACTGCGGG TCTTGCCACA - 10021 TACGTCCAGA GGTTCGCTTC GCTCTTCGTC GGTCCAGCTC TCGCTGTAGG TGAGTGGCTG - 10081 CGACTGCTGC AGGTGCTGTT CCCCGAAATC CAACGGCGGT ATGCAGATGC TGCCGCCTTG - 10141 GGCAGGGACT TCTACGACTC CCAACGCGCA CTACACCACC CAGAGCTGCC CCGGAACGAG - 10201 AGGTTCCGGG GAGAGCTTCG GTGGGAGTGG TTCGTCCAGA ACATGGAGCC CGCTCGAAAA - 10261 GAGATGTCGC AGGCCGACTC TCCGCCGAGT GCGACCTCTA AGTTGGCTCT GGCCGCAGTT - 10321 CGCGAAGTGG AGATGGCAGC ACGCCGACAG ATCATCGGCG CTGTCAAGAA CGATCCGGCC - 10381 CCGCAGATCG TGCAGGGCTG GGCGAGGGTC GCCACCGGGC GCGAAACATG CGCCTGGTGT - 10441 CTGATGCTCA TCTCACGGGG TGCCGAGCTG AATCACAAGG GCAACTTCGC CTACAGCTCA - 10501 GCGGAAGCCG CAGGGCTCAA CCTCGATGAC GAGACCGTGA TCGACCTCTG GAACGAGTCC - 10561 GGTCACGACC TTGAGAAGTT CCGCGAGGAG ACCAGAGAGG ACTTCGAGAA GTGGCACGCA - 10621 GGGTGCGACT GTCTGGTGGT CCCGGTCTTC GATGTGCAGA ACTGGCCCGG AAGAGACGCT - 10681 GCCCTACGGG CGCAGCAACT TTGGATCGAA GCCAGCGACG AAGCTGACGA CCTCATTGCG - 10741 TCAGGCAAGG CCCGCTCCAA GAACAAGAAC ACGGAGACGC TCAACGCGCT CCGACGCCGC - 10801 CTAGCACGCG GCGAAATCAC CATGTCCAAC TACGCCCTCG CTGCGTAGTC CCTCGAACCC - 10861 CAGGTGGGTT CTCTCAACAT GCCCAGGAGG CGAAAACACA TGTCCGACAA CCCCACTCCC - 10921 GAGAGCACCC CAGAGGCCGA GACCCCGGAG GTCGAGAAGC CGATGGAACC GCAGGGCAAG - 10981 GTCTTCGATG AAGCGTACGT TCAGTCGCTT CGCCAGGAGG CTGCAGCCGC TCGGGTGGCG - 11041 AAGAAGGACG CCGTAGAAGC GGCAGAGGCT CGAGTGAAGG CCGAGTACGA GGCCAAGCTC - 11101 GCTGAGCGCG ACACCGCTTA CACCGAACTG CAGAACCAGT TGGGACAGGC GTGGATTGAG - 11161 CTGGAGAAGG TCTACCTCTC TCTCGACGCC AAGGTGCCCA ACGACAAGGT TCGGGCGTTT - 11221 GTCGAGATCC TCGAAGGCAA CGACAGGGAC AGCATCGCTG AGTCAGTGAA GTCCCGTCTG - 11281 GAGCTGGTCG GCGGATTCGG CAACAAGACC CCGAGTCCTG CGTTCGACCC GTCTCAGGGT - 11341 CGCGGCGGTA AGCCGCCGAT CCCGCTGAAC GGTGACCCGA TCCTCGAGGC CATCAAGGCC - 11401 GCTGTCGGGA TCAAGAAGTA ACCCACCCAA CAGATCTCAA GGAGAGATAA ACAATGGCAG - 11461 TCAACCCTGA CCGCACCACG CCGTTCCTCG GCGTGAACGA CCCCAAGGTC GCGCAGACCG - 11521 GCGACTCGAT GTTCGAGGGC TACCTCGAGC CCGAGCAGGC CCAGGACTAC TTCGCCGAAG - 11581 CGGAGAAGAT CTCCATCGTC CAGCAGTTCG CCCAGAAGAT CCCGATGGGC ACGACCGGCC - 11641 AGAAGATCCC GCACTGGACC GGCGACGTGA GTGCGTCGTG GATCGGTGAA GGCGACATGA - 11701 AGCCCATCAC CAAGGGCAAC ATGACCTCGC AGACCATCGC CCCCCACAAG ATCGCGACGA - 11761 TCTTCGTGGC CTCGGCGGAA ACCGTCCGTG CGAACCCGGC CAACTACCTG GGCACCATGC - 11821 GGACCAAGGT CGCGACCGCC TTCGCGATGG CGTTCGACAA CGCCGCGATC AACGGCACCG - 11881 ACAGCCCGTT CCCGACCTTC CTAGCGCAGA CCACCAAGGA GGTCTCGCTG GTGGACCCGG - 11941 ACGGCACCGG CTCCAACGCC GACCTCACCG TCTACGACGC GGTCGCCGTC AACGCCCTGT - 12001 CGCTGTTGGT CAATGCCGGC AAGAAGTGGA CCCACACTCT GCTGGACGAC ATCACCGAGC - 12061 CGATCCTCAA CGGCGCGAAG GACAAGAGCG GTCGCCCGCT GTTCATCGAG TCGACCTACA - 12121 CCGAGGAGAA CAGCCCGTTC CGCCTCGGTC GGATTGTGGC CCGTCCGACC ATCCTGAGCG - 12181 ACCACGTCGC CTCGGGCACG GTCGTCGGCT ACCAGGGTGA CTTCCGCCAG CTCGTCTGGG - 12241 GCCAGGTCGG CGGCCTGTCC TTCGACGTGA CGGATCAGGC GACTCTGAAC CTGGGCACCC - 12301 CCCAGGCTCC GAACTTCGTC TCGCTGTGGC AGCACAACCT CGTCGCAGTC CGAGTCGAGG - 12361 CCGAGTACGC CTTCCACTGC AACGACAAGG ACGCGTTCGT CAAGCTCACG AACGTGGACG - 12421 CCACCGAAGC CTGATCCAGG CTTGACATCC ACCGGGAGGG GGCTCCTTCG GGAGCCCTCT - 12481 CCTGATGTGG AGCAGGAAGG ACCACATGCG AATCCAGTCC ACCCTCAACG GCGGTTTCGC - 12541 CGAGGTTTCC GAGGAGTTCG CCAAGCAGTT GATCGCCACT GGCGGCTGGA AGGTGCCCCG - 12601 GAAACCGCGC AACACCAAGA CCAAGACCGC TCCTGAGGAG CCCAAGAACG AGGAGTAACC - 12661 CGTGGCCTAC GCGACCGCCG AAGACGTTGT GACGTTGTGG GCCAAGGAGC CTGAGCCCGA - 12721 AGTGATGGCG CTGATCGAGC GCCGGCTCCA GCAGATCGAG CGCATGATCA AGCGCCGGAT - 12781 CCCCGACCTG GACGTGAAAG CCGCTGCGTC GGCGACGTTC CGGGCCGATC TGATCGACAT - 12841 CGAAGCTGAT GCTGTTCTGC GCCTCGTGCG TAACCCGGAG GGCTACCTCT CGGAGACCGA - 12901 CGGTGCGTAC ACCTATCAGC TCCAGGCCGA CCTGTCGCAA GGCAAGCTCA CCATCCTCGA - 12961 TGAGGAGTGG GAGATCCTCG GGGTCAACTC CCAGAAGCGC ATGGCGGTCA TCGTCCCGAA - 13021 CGTGGTGATG CCGACGTGAG CGCGAGCGAC CGACACCGCG CCCCGATTGT CTATCCGCCT - 13081 GGCACTCAGG CGGTTACGCC GGATCGGGTC AACGCGTTTG ACTGCGATCA CGAAGCTGAT - 13141 CCTCCGGTGT GCCGGTGCGT CCACGACTGG CGCATCGAGT GGGGAAACGT CAAGAAGGCC - 13201 ACCGCCAGAT CACGGTCGGC GGTGCTCTGA TGAGCCTCCT CGACACCGGT GCCCGGTACC - 13261 AGACCTGCAT CGTCTACCCC GAAGAGATGG TCATCGACTC CGATGGCAAC AAGCGGACCA - 13321 GGCCGTCGAA TACCGGCATC CCGGCCATCG CACGGTTCCA GGTAGCCAAC CAGTCTGGTA - 13381 CGTCGGCACG ACGTGCTGAG CAGGACAACG AGGGGTTCGA GACCGAGAAG GTCTACCGGA - 13441 TGCGGTTTCC CCGCTCGTTC ACCAAGGAGC ACGGCATCCT CGGGGCCCAG TCCCAGATCG - 13501 AGTGGCGAGA CCAGCGGTGG GCGCTCTTCG GAGACGCCAC CGTCTACGAC TCATCCCCTG - 13561 CGTTGGCGCG GGTCGACTAC ACGATCAAGA GGTACTGATG GCCAAGGTCT ACGCGAACGC - 13621 GAACAAGGTC GCGGCCCGGT ACGTCGAGAC GAGGGACGCC GTCCGAGACG AGCGGAACAA - 13681 GGTCACCCGT CGAGCCAAAG CCAATCTGGC GCGGCAGAAC TCGACCACCC GCATCACCGA - 13741 CGAGGGCTAC TTCCCGGCCA CCATCACCGA GCAAGACGGC GATGTCGACT TCCACACGAT - 13801 CCTCAACGCG CCCAACGCGT TGGCGCTTGA GTTCGGCCAC GCGCCGTCTG GCTTCTTCGC - 13861 TGGCACCGAC ACGAAACCAC CGGAGGCCAC TTACATCCTC ACCCGAGCCG CCATCGGCGG - 13921 CACCGTCTCA TAAGGAGGTC ACATGGCGCG AATGCCTCGC GTCCAGGCAG TAGCGGCCCC - 13981 GATCCTCCGG TCAGACCCCC GACTGGAGGG AGTGACGGTC ACGACATGGG TTCCAGACGT - 14041 GGACTTCCGA GAGTTCCCGA TGATCAACCT CCGCCGCATA GGCGGGACGA GGAACCCCAA - 14101 CGCACCGACG CTGCACACGC TGCCGGTGGT CGAAATGACC GCCTACACCA GAGACGGTCT - 14161 CATCGAGACT GAGGAGCTGT ACGAGACCGC GCTAGAGGTT CTCTACGACG CGGTGGAGAA - 14221 CGGAACACAA ACTCCCGCAG GGTATTTGAC CTCCATCTTC GAGACGATGG GCGCCACTCA - 14281 GTTCAGCTCC CTCTACCAGG ACTCCTGGCG CATCCAGGGT CTGATCAGGC TCGGCGTCCG - 14341 CAGACCGAGA ACCACCCTCT AACCGAAAGG TAAAGCCACA TGGCTGAAAA CGACGACGCA - 14401 GTGTTGACTG CGGCGGTCGG CTACGTGTAC GTCGGTGCTG CAGGCACCGC TGCTCCTACG - 14461 CCGGCCTTGC TCAAGACCAT CGACCTCAGC AAGCCCGAGA CCTGGACCGG TGCTACCGGT - 14521 TGGACGAGCG TCGGCCACAC CAGCCGAGGC ACGCTCCCTG AGTTCGGCTT CGAAGGCGGC - 14581 GAGTCCGAGG TCAAGGGCTC CTGGCAGAAG AAGAAGCTCC GCGAGATCAC CACCGAGGAT - 14641 CCCATCGACT ACGTCACGGT CCTACTGCAC CAGTTCGATG AGCAGTCGCT GGGTCTGTAC - 14701 TACGGCCCCA ACGCCTCTGA GACTCCTGGT GTGTTCGGTG TGAAGACCGG CCAGACCAAC - 14761 GAGAAGGCCG TGCTGGTCGT GATCGAAGAC GGCGACATGC GCCTGGGGCA TCACGCCCAC - 14821 AAGGCTGGAG TTCGCCGCGA CGACGCGATT GAGCTGCCCA TCGATGACCT GGCTGCGCTG - 14881 CCCGTCCGGT TCACCTACCT GGACCACGAA GACGAGCTGC CGTTCTCCTG GATCAACGAA - 14941 GACCTCTTCA ACGTGCCCGA GGTTCCCGAG GGCTGATCCC AACTTGACAG CCACCCGGCT - l5001 GTCTACCCCG GAGGGGGAGG TTTCCTTGGC GGGCCTGGCC TCCCCCTCCT CCCGCCACTC - 15061 ACAGACCCGC CGACACTGAA AGGTTCGCCA TGACAAACGT ATTCACCATC GACGCATTCC - 15121 GCGAAGAGGT CAAGAAGAAG TACGCTCCGG TCCTCATCGG CCTGTCCGAC GATGTGACCG - 15181 TCGAGCTGAA GCCGCTGCTG AAGCTGGGCC AGAAGGCCCG CGAAGCGGTG GTCGAGGTGT - 15241 TCAAGGAGTT CGCGGACATC CCCGACCTCG AAGAGGACGA CGACGACGAG TTGGTCGATG - 15301 AGTACTCGCT CCAGGTCTGC GACATCATCG CCAAGGCGTT CCGGCTGATC GCCACGAAGC - 15361 CCAAGAAGCT GATCGCCGCC TTGGACGAGG AGCCGGATCC CCGTATCCGC GCAGAGCTGT - 15421 ATGCAGCGGT ACTCAACACC TGGAAGCGAG AGACGCAACT GGGGGAAGCC GCGCCCTCGC - 15481 CGAGCTGATC GACAAGTTCG GCGGGGCGAT CCTCGCAGAC CTGCTCCAGT ACTACCGGGT - 15541 AGACCTGCGC GACCTGTTCC GCGACGAGGA TCCGCTTTCG CCGAGATTCG TTCTGTCCCT - 15601 GGTGCTCTGC CTTCCCAAAG ACGGCGCGTT CTACGCAGAA CGTCGTGGTG GGCAGCAGTA - 15661 CCGGGGCTGG ACCGAGGACC GCTACGCGCT CGCGGACATC TACGACGCCA TCCAGGCGGG - 15721 CAACCACATC CTGCTGCTGG CGAATCGTGA TCCGAAGAAG CCAAAGCCCA AGGCACCCAA - 15781 GTCATACCCG CGTCCCGACG ACCTAGAGAA GACCACACCG AAGCCGGGTT CGTTCGCCGC - 15841 AATGGTCGTG CGAGCGAAGA AGGCGGCTCG AGAGAGAAGG GAAAGGGAGG AGGAGAGTGC - 15901 CGAATAGTGC TGGCGTAGAA GTCGCCCGGA TCTCGGTCAA GGTCAGCCCG AACACCAAGG - 15961 AGTTCCGCCG GGAACTCAAG ACCGAACTCG AGAAGATCGA GCGGGAGCTT AAGGGCGATG - 16021 TCGAGATCAA CGGTCATCTC GATGCGGCCC AGGCCAAGGC CGACTTCAAG CGCATGATGA - 16081 TGCAGCTCAA GACCGAAGCT GCCAAGGGCG TTCACGTCCC GGTCGACGTA ACCGTCGACA - 16141 AGAAGAGCAA GAAGGGAGGT CTCCTCGGAG GTCTCCTCGG CGGCAGCCGG GGGCTCGGAG - 16201 ATCTAGGCGA TGACGCCGAG AAGGCGTCGT CTCAAGTACA ACACCTTGGC AAGTCGTTCC - 16261 TGGGCCTCAC ACGAGCCGCC TGGATAGGCG TAGGCATCGT CGCCGTAGCA GCTCCGCTGG - 16321 TCGGCATCGT GGCCGGTCTG CTGGCCGGTC TGCCGTCGCT GCTGTCTGCG TTCGGAGCCG - 16381 GCGCTGGCGT AGTCGCGCTC GGCATGGACG GCATCAAGGC AGCCGCCTCG ACGCTGGCCC - 16441 CGACGCTGGA GACGGTCAAG GCCGCTGTCT CCTCGACGTT CCAGCAGGGA CTCACCCCGG - 16501 TGTTCCAGCA GCTCGGCCCG ATGCTGACCG CGATCACCCC CAACCTGCAG AACGTGGCCT - 16561 CGGGCCTCGT GAACATGGCC GGGTCGATCA CCGACGTGAT CACCCAGGCT CCTGGTCTGC - 16621 AGCAGATCCA GAACATCCTC ACCAAGACCG GAGAGTTCTT CACGGGCCTC GGCCCTGTGC - 16681 TCGCTACCGG CACGCAGGCG TTCCTGACGC TGTCCAACGC CGGCGCGAAC TCGTTCGGCA - 16741 CGCTCCTGGC TCCCCTGCAG GAGTTCACCA ACGGCTTCAA CGACATGGTC AACCGAGTCA - 16801 CGTCCAACGG CGTGTTCGAG GGTGCCATGC AAGGGCTTTC GCAGACGCTG GGCAGCGTCC - 16861 TCAACCTGTT CAACCGGCTC ATGGAGTCCG GTCTGCAGGC GATGGGACAG CTCGGCGGTC - 16921 CGCTGTCGAC GTTCATCAAC GGGTTCGGAG ATCTCTTCGT CTCGCTGATG CCGGCGCTGA - 16981 CTTCGGTCTC TGGTCTGATC GGCAACGTCC TCGGGACGCT GGGCACACAG CTCGCTCCCA - 17041 TCGTCACGGC GCTCACGCCG GCCTTCCAGA CGCTGGCGAG CACGCTCGGC ACGATGCTCA - 17101 CCGGAGCCCT CCAAGCTCTG GGTCCGATCC TGACTCAGGT CGCTACGTTG ATCGGCACGA - 17161 CGCTGAACAC GGCGCTGCAG GCTCTCCAGC CGATGCTGCC GTCGCTCATG CAGAGCTTCC - 17221 AGCAGATCTC CGACGTACTG GTGACCAGTC TGGCCCCGCA CATCCCGGCG CTGGCGACGG - 17281 CCCTCGGCCA GGTCGCAGGC GCGGTGCTGC AGCTCGCTCC GACGATCATC TCGACGTTGG - 17341 TTCCGGCGTT CGTTCAGTTG GTCCCAAAGG TCGCTGAGCT AGTTCCGACC ATCGTCAACC - 17401 TGGTCCAGTC GTTCGCCAAC CTGATGCCGG TGGTTCTGCC CCTGGCGCAG GCTCTGGTCA - 17461 GCGTTGCTGG CGCGGTGATT TCTCCATCGG CAGGTGGGTG CGGCGCGCTC ATCGGCGCGC - 17521 TGGCGAACCT CACGGAGATC ATCTCCAACG TCATCAAGAA GGTGTCCGAG TGGGTCAGCA - 17581 GCTTCTCCAG CGGAGCCCAG CAGATCGCTG CGAAGGGCAG GGAACTGCCG GGGATGATCC - 17641 AGTCGGCTCT CGCCAACCTG ATGGCCATCG GCCTGCAGGC CGGTAAGGAT CTCGTCCAGG - 17701 GCCTGATCAA CGGCATCGGC GGGATGGTCA GCGCAGCGGT CAACAAGGCC AAGGAGCTGG - 17761 CGTCCAGCGT GGCGGGTGCA GTGAAGGGCT TCCTGGGCAT CGAGTCCCCG TCGAAGTTGT - 17821 TCACCGAGTA CGGCCAGTTC ACCGCCGAGG GATTCGGCAA CGGCATGGAG GCAGGGTTCA - 17881 AGCCCGTCAT CGAACGGGCC AAGGATCTCG CGGCTGAGCT GTCCAGGGCG ATGGAGTCGG - 17941 GCACCGACCC CTCCGGGATT CTCGCGGGGC TGGATCAGAA TGAGCTGAAG CAGATGCTGG - 18001 CGGCTCTCGA AGAGGAGCGC AAGCGACTCA AGGTCGAGAA GAACGGTATC CCCAAGGGAG - 18061 ACAAGGCAGG CCGAGAGGCG CTGCAGAACC AGCTCGACCA GATCCAGGCG CAGAAGGACA - 18121 TCCTGTCCTA CCAGCGTGAC CGCATCAAGA ACGAGTCTGA GTACGGCGAC ATGGCCGGCG - 18181 AAGACCCGTT GGTGAAGGCA GCCTCCGGGC TGATGAGCGC ACCGGTCGAC TTCGCGAAAG - 18241 CGACTGGCAA GCAGTTCCTT TCGGACATCG GCATCAGCGG AGATGGGTTC ATCTCGAAGG - 18301 CCATCACCGA GGGCATCCAG TACATCTTCC AGATCGGCTC TGTCGATGAG GCGCTGTCGA - 18361 TCAAGGACCG CGAGGAGTCG AAGAACGCGC TGTCCGTCGT TGGCCGCTGA CTTGACATCC - 18421 ACCAGGAGGT AAGCATTGAT CACCGACACC ATCGTTGAAC TCGAGGGTGT CAATGGTGAG - 18481 CGTTTCAACT TGACGACCGG TGACCAGGGT GTGTACCTGG CCACAGACGT GGAGGGTTGT - 18541 TTCTACGACC CTCCCGTCAA GGTCGTTGTT GAAGAGCCGG GGAACTACCC CGGCGCTCGC - 18601 TACTTGTCCC ACCGAGCCCT GAAGCGAGAC ATCGTCTTTG GGGTCGTCAT CCTCAACGAC - 18661 GCGAAGCAGG GGCCGCGCTC CTGGCTGTCG CGAGACTCCG AGTGGCGCAA GGCGTGGGCG - 18721 TTCAACCGCA CCTGCAAGCT CTACGTCACC ACCCCGGACT CCGGTACCCG CTACCTGAAG - 18781 CTGGCGCTGT TCGAGTCCCC CACCGTCAAG ATGGACACCG ACCCAAGAGG TAAACCCCTT - 18841 GAGGTCACGG TGATGTCGTG CATCGCGTAC GACCCGTTCT GGTACGAGGA CGACAAGGTC - 18901 TTCTCGGCCA AGACCAAGAC CGACACCCGG TTCGACCCGT CGTTCTGGAC GCCGCCGTGG - 18961 CCGTGGGAGG AACTGCCCAA GGAGACGCTG CGGATCAAGG TCGGCCGCGA GCAGGGTGGG - 19021 CTAAACCCCA CCGACCAGTA CATCTTCCCG AAGTGGACCG TTCCCGGCTC CACCGAGAAG - 19081 GTGCCGAACT TCCCCTGGCC GTTCCCCCCG AACGTCCCGA TCCCGTGGGA GACAGCACCG - 19141 TTCACTCAGT TCGTCATCCC GGACTACTCG TTCGAGGATG AGGAGTTCCG CAACCGCCGG - 19201 CTCAAGACGC CGGGGTTGAT CTACGGCGAG AACTGCGTCA TCGACACCGA CCGGCGCGAG - 19261 GAGCAGATCG CTTCCGAGTC GGGCTCCCCG GTGTGGGCTC GGATGAACGG TGTCCGGTTC - 19321 CGCAACTCGA TCCCGCCCTA CACCGAAGAG GCTGAGTTCG TCATAGACGC ATCGGGATGC - 19381 GCTCCGGGAC AGGTAGTTAC CCTCCGGCTC ACGAGGCCGT GGTCGCGCTG CTGGGGGCTA - 19441 GAGTGAGTGG TCTGACGAGC GTTCGTGAGG CCGAAGATCT CTGGCAGAAG ATCCAATTGC - 19501 GGCGCTGCAA GCGCGAGCAG GAACGGCTCA AGCATCCCGA CGTAGAGCTG CGCGATGGCG - 19561 ACTTCCGCCT GCGCGGCCTG GTCGCTGGCG AGCGGGTGCT CGAGTGGGAG TTCATCGAGA - 19621 ACGAGACTGG CACCTGCACC TTGCAGCTCT CACTGAGCCA TTACCTGGCG AAGTGGGTGA - 19681 TGGACCACCG GGGTCGAGCA AAGCGCAACG TCATCATCAA CATCGAGAAG CAAGGCGCTC - 19741 GATGGACCGG GATGATGGAC CACTACCGGG TCATCAAGAC CGACGCAGGG GACGCCTACA - 19801 TCGAGATCGT GTTTTTGCAC GACTTCGAGC AGACCAAGCA TATCCGGGTA TGGTGCAACC - 19861 CGTTCCTACG CCCCGAGCTG CAGTTCCCCA AGGTGTGGAT CATCTTCGGG CCGGCCAAGT - 19921 GGTGTTTGCT GGTGACACTG TTCGTCAACC TGCTCAGGCT CGAGACGAGC TTGTGGACGC - 19981 TGCCTGATGA CCCCACGGAC ATCAACGAGT GGATGGGTCC GAGCTTCAAC CCAGCAAATT - 20041 GGCGGAACAT CGTCAAGCCG TTCCCGTTCC TGGCCGACAA CTCACCGGTC ACGATGGTGT - 20101 TCAGCCGGTT CGGGACGTTC TACGACACCG CCAAGAAGAT CCTCGAAGAC CATCAGCTCA - 20161 CGCTGACGTG TCGTCGGTAC ATCAAGGACC GCGACCCGCA TCCGTTCGAA GATCTCAAGG - 20221 GGCTCTGGGG AATTGATCCT GTCGAAGACC TGCTGCAGAA GATCCCGCTC CGGGACGGCT - 20281 GCGTGGTCTG GGACATCGAG GACAACTCAG GTTGGGGCAC TCAGACCGCG TTCGGCGGTT - 20341 CGTGGCTGAC CGGGTTCGTC CGAGGGATGG TCCAACTGGC CGGCGACGGC CAGGTCGAGG - 20401 GCGTCGATGT GTTCACCGGG GACTACACGT TCCCAGGCGA GTACTACTCC CCCTGGTTCA - 20461 TGGGCACCAG CCCGATAGCA CCCCACGTCG TGTTCGAAGA AGGACCGCTG ACCGGGATCA - 20521 AGTCGTCGGA GTTCTCGTAC TACGAGGCCA CCGACACCAG CTTCCTGGCT GGTGGACAGA - 20581 GCGCACCTGG CATCAACGAG GGCATCTCGG CCCTGGTGAA CATCGGTGGC GACCTGCTGA - 20641 CCTCGTTCAT CAACAGCCAG CTCGCCGCGC TCGGCGCGGT CGGTGGAGCG ATTGACCTCC - 20701 CGCCTCTGGG CGGTCTGCTC GATGCGGTGT TGCAGCCTCT GTACTCCGAT GTGTTCGGCG - 20761 CGTTCATGGA AGTTCCGACT CTGCGTGCGA TGGGCATCTC GCTCCCGATC TCCGGGCTCG - 20821 AGGACATCGT CACCGGACTG GGCGACTTCC ACTACTTCGA GAACATGGCC GACGGGGCGA - 20881 TGAAGGCGTT CACGCTGTCA GCGTTCGCAG CCATCGCATC GCAGATCCAC AAGACGAGGG - 20941 CTCGAACGAC CCACACCCTC AAGGTGTCTG ACGCCGCTCC GTACATCTTC GCGCCAAAGC - 21001 CCTACGGGCA CTGCTGGATC GGAGATCGCG TCGGCACGTC GGTCCTCGGC TACCCGGTCG - 21061 AGCACCAGTT GTTCGTGGAG CGCATCCGCA AGGTGAAGTA CCGCATCGAC AAAGACGGCA - 21121 TGAAGCCGTT GGAGATCGAG ATCGGTTACC GCGAACCGAA GAACCCAGCA CTACACATCC - 21181 TCGAAGAGAT CAAGCGCGTC AACGGCGCTC TTGGCACTGC GGGGATTCTC TAAACCGAAA - 21241 GGCACGCCGC ATGATTCCCT CACAAGAGTC TCACAATCCG AACGACCCGC GACAGCACGT - 21301 CATGTGGGCG CTACGCAATC TCCCGATGAT TGCTGGCGTC GGGGCGATCA CGCATCCGGG - 21361 TTACCTGGCG GATTGGTCAG AGCACTTGTG GAAGTGCGGC TTTCGGCACG TCGACTGGCT - 21421 CCGGGAGCTG GCTGATGAGG ACGGCAACAT CCACGTCAGT CAGCTTCCTG ACCAGGAGAT - 21481 CAAGTTTCAG CAGCCCTTCC GGGGCCAGCG AAGCGACTAC AACAACGCAG CTCGATGGGT - 21541 CGGCAAAGAC GATCCTGACC CAGAGCCCGT GCGTATTCCA GACATTCGCA AGCTCACAGA - 21601 CCAGGAGAAC AGAGCGATGA TCGCGCAGTA CGAACGAGAC GGTTGGATCA AGGATGGATC - 21661 CCCCGGCCCA GCGATAGCCG AGGTCGTGGA GTGACCCCGT TCAACCCAGA CTCCATAGGC - 21721 GACTACGTGA CACTGCTCGG CGTTGCGTTC CTGACCTTCT CGGTTCCCGC ATGGTTCACC - 21781 GGACGAGCAC GCAAGCACAG CAGTGACATC GGCGAAATCA AGGAACAGGT ATGTAACACC - 21841 CACGACACGA ACCTGCGCGA TGACCTCGAC AGCGTCAAGG CAGACATCAG CGACTTGAAA - 21901 GAGATTGTGT TGCAAGGGTT CCACCAGGTG AACGAGTCGA TCAACCTCGA GCGCCGTGAG - 21961 CGGATCGAAG GAGACCGCCG AAAGGAGGTT GCGTGACCTA CCCCACCAAC CCACTAGAGG - 22021 CCATCGGCGC TGACGGCGCA TTCGAGATCG GTGGGGGCGA CTGGAGCTTC GGCCAGGACT - 22081 ACACCGAACA GGCCATCCGG GCTCTGTTCA CGATGCCAGC GGTCACGATG GAGAACGCTC - 22141 TCGGCCTGCT CGAAGAGCAC CTGCTGAAGC TGCCTCTGGA GGCGCTGCAG GGCTTCAAAG - 22201 ACATGATCCC GGACTGGGTC GAAGGAGCAT TCGACACGGT CACCGGCGCT GTGCAGGCGA - 22261 TCATGAACGC GCTCCAAGAC GGCCCGCTGT TCCTGAAGTT CGCCGAGTTC CAGCTCTTCC - 22321 TGCAGCGTCT GCTGAACAAC CCGGCCGAGG TCATCGGCGA GATCCCCCAG ACGTTGATCG - 22381 ACGGCCTACA GGACGCGCTC AACACCGTCA ACAACACCAT CCAGACCATC GTGGACATGC - 22441 TCCTGCAGGC GCTGGGCATC ACCCCGGAGG GGGAGCTGAT CGACCGGATC TTCGACCTGA - 22501 GCGATGAGAT GGAGTGGCTG CAGACCGCAG CCTCGAATGC AGCTACCGGC ATCCAGGACA - 22561 CCTGGAACAA GTTCTGGGGA GCCCTCACCG GGCGCGTCCC AGACCAGGAC CAGACCGTCG - 22621 CTGAGCCCGC CGAGCGTATC GGCGAGCTGG CCGGCACCAC GTCTGCTAAC TCGTCTGCCA - 22681 TCGCGGAGCT GCAGCGTCGA CTGGACAACC AGCAGAACGC TGGCGGCGTG GCCGGCGGTG - 22741 ACGACTTCGA GCGACTGAAC ATATCCGGTT GGGACATCAG GTATTCCAAC GGATCCAGCG - 22801 GCCGAGGGTA CTACCGTGCC GACGGCCACC AACTGGTCTG GATGGACGAA GGCAACCAGC - 22861 AGAACACCGC GACGTTCGTC CGCACCAACC CCGCAGACGA GAAGACAGCC ACCGACTACC - 22921 AGAAGATGAC GTTGGTCGTC GGGACTATCT CCGGTGAGGT ACAGACCGTG TTCCCGCCGC - 22981 AGGGAGGTTC GCACACCCGG CTATGGGTCC GCGTCAACGA CAACGCTCCG ACCGTCGGCA - 23041 TCACCGACGG CGTGTTCGTA GAGATCGGCG GCGTATCGAA GGCCCAGATC GGCTACCGCC - 23101 GCAACGGCAA TGACACGTTC GTCGGATCTA TGGTCGACTG CACCTGGGGT GCTGGATCGA - 23161 TCTTCGCTCT GACCGCCGGC ACGGCCAACG GTGCTGAGAA GTTCGAGGTC TCGAAGAACG - 23221 GCCCCGTGCT GGCCACATGG TCGGACGACG GCGTCGTCTC CGCGATGGGT GCGAACTACC - 23281 GCCGCTGGGG CTGGGAAGGC CAGGCTCGTA ACCGCAACCT CGGCCAGGGC ACTCCGAACT - 23341 CGGTCACCCG AGTGACGATC ACCGACAACG ATCCTACCGG CGCAGGCGGT GGAGCTGTCA - 23401 ACGTCGGAGG AGATGTCGTA GGTGTACTCC CCATAGAGAA CGGAGGCACC GGAGCTTCGA - 23461 CAGCTTCGGC AGCCCGTACC GCTCTCGGAA TCGATGACCT GGTCGAAGAT ATGTCCGACG - 23521 TAGTTCGTGG ATCCGTCGAA GGACTCCCGT TGATACCGAA GATCTGGGTA GGAACAGAAG - 23581 CTCAGTACAC GGCTCTCGCC ACCAAGGATC AGTCCACGCT ATACTTCAGG ACCGCTTAAT - 23641 GACTGGTATC TCGTTGGGTG TCAACGACAT CCGCAACCTC TCGATATTCT TAGGCGTCAG - 23701 CAACAAGATA TTGAAGGTCA GTCTAGGCAC AGAAAAGGTC TGGCCTGCGT TCACCCCGGT - 23761 GCTGACCACG TTCGCCACGG TCGGCACGTA CACCTACAAC ATCCCCGACG GGGCCAAGTT - 23821 CATCGACGTC ATCCTCCTCG GAGGAGGCGG CGGGGGTAAA GGCATGGCCC TGGCTGACGG - 23881 CTGGGGCAGA GGTGGAGACG CCGGAAGCTG GGCTATCGTC ACTCTCGAAC GCGGGGTACA - 23941 CATCCCGTTG TCGACCAAGA CGATCACCGG GCTCGTCGGA GCTGGAGGCG CAGCGGGAGC - 24001 TGGCTCTGTA TTCTCAGGCA AGGCCGGAGG CCCTGGAGGA AACACCACGG CGTCCGCTGT - 24061 CGGATGGTCA GGTTTGACCG CAACCGGCGG TCCCGGAGGC TCTGTGATCG ACATCCTCAG - 24121 CGTCGCCGGA AAGTCGCCTG GAGATCGGAC CTACAACGAC CAGCTCTACA TAGGCGGCGC - 24181 ACAACAGAAC TCAGCTGGCG GGAACGGCAA TGCTCCTGGC GGCGGCGGGG CTGGTGCCCA - 24241 GGTCTCCGCA CAGAGCGGCG GTGCTGGCGC TCGCGGCCAG GCGTGGTTCT TCGCGTACTG - 24301 ACAAGAAACC CCCCTCTTTA GGACTCAGTG TCCTTGGGAG GGGGGCTTTT TGCGTTTCAG - 24361 GAGGTCTTGG CCAGCTTGGA CATCGCCTCA GCGATAGCCT CGTCGCGGGC CTCAGACGCC - 24421 ATCTGGTACT TCATCGCCAT CCTAGGAGTC GTGTGACCGA GACGGGCCAT CAGCTCCTTG - 24481 GTCGTCGCAC CTGCCTGAGC GGCGAACGTA GCGCCGACAG CGCGGAGGTC GTGGATGCGG - 24541 AGTTCCGGCC GACCGATCTT GGCGTAGCCA CGCTTCAGCG ACTGGGTGAA CGCGGACTTC - 24601 GACAGCCGGT TGCCCTGCGT CGTGGTCACC AGGAATGCCT CGGGGCCCTT GTTCATCTTC - 24661 GTACGGTCCT TCATGTGCGC TCGGATCATC TCCGCGACGT GAGGCGGAAC CGTCACAGGA - 24721 CGCTTCGACC GGACGGTCTT GGCGTTGCCA ACGACGATCT TGTTCCCCAC GCGGGAAGCG - 24781 CCACGGCGCA CCCGGAGCTT CATCGTCATG CCGTCGTCCA CGATGTCCTI GCGGCGAAGC - 24841 TCGATCAGCT CTCCGAACCG GAGGCTCGTC CACGCCAGGA TGTATGCCGC GATCCGGTAG - 24901 TGCTCGAAGA TCTCAGCGGC GACGATGTCC AGCTCCTCAG GCGTCAGCGC CTCTACGTCG - 24961 CGCTCATCGG CTGCCTTCTG CTCGATCCGG CACGGGTTCT CTGCGATCAG CTTGTCCTCG - 25021 ACCGCTGTGT TCATCACCGC CCGGAGGACG TTGTAGGCAT GCCGGCGGGC AGTCGGGTGC - 25081 TTCCTACCCA TCCCGGCCCA CCACGCACGC ACCAGAGCTG GCGTCATCTC TGTGACCGCC - 25141 ACTTCACCTA GCACCGGGTA GATGCGGCGC TCCGCGTGCC CGCTGTACAG ATCCCGGGTG - 25201 CCGTCTGCGA GGTCGCGCTC CACGAGCCAC TTCCGGGTGT ACTCCTCCAG CGTGATGGCG - 25261 CTGGCGGCTG CCTTCTTCGC CCGGTCCTGT GGAGGGGTCC AGGTCTCCAT CTCGATGAGC - 25321 CGCTTCTCGC CCGCGAGCCA GGCTTCGGCG TCCATCTTGT TGTCGTAGGT CTGCAGCGCG - 25381 TAGTACCTCA CACCGTCCTG CGGGTTGACG TATGAGGCTT GGATCCTCCC GCTGCGCTGA - 25441 GTCTTCAGCG ATCCCCATCC GCGACGTGCC AACTAGGTCT CCTCTCGTCG TGAACAAGGC - 25501 TACCGGGTTG CAACTCCTGT GCAACTCTCA GGCTTCAACG CGCTTCTACG ACCTGCAATT - 25561 TCTTTCCACT TAGAGGATGC AGCCGAGAGG GGGTAAAAAC CTATCTTGAC CGGCCCATAT - 25621 GTGGTCGGCA GACACCCATT CTTCCAAACT AGCTACGCGG GTTCGATTCC CGTCGCCCGC - 25681 TCCGCTGGTC AGAGGGTGTT TTCGCCCTCT GGCCATTTTT CTTTCCAGGG GTCTGCAACT - 25741 CTTGTGCGAC TCTTCTGACC TGGGCATACG CGGTTGCAAC GCATCCCTGA TCTGGCTACT - 25801 TTCGATGCTG ACAAACGAAT AGAGCCCCCC GCCTGCGCGA ACAGACGAGG GGCATTCACA - 25861 CCAGATTGGA GCTGGTGCAG TGAAGAGAAT AGACCGGGAC AAGGTTGCAC CGGGAGTTGC - 25921 AGCGGTCGGA ACCCTCGCCG TCGGCGGGCT GGCGTTCGCC CTGTCGTTCA CGGCTCTCAG - 25981 CGAGCTGGCT GCGGCCAACG GGGTGGCCCA AGCAGAGATG GTGCCCTTGG TGGTCGACGG - 26041 CCTGACGCTC GTCGCCACGG TCGCCACAGT GGCCCTCAAG CAGAACAGTT GGTACGCGTG - 26101 GTCGCTGCTG ATCCTGTCCA CCGTCGTATC GGTGGCCGGC AACGTGGCAC ACGCCTACCC - 26161 CCACGGCATC ATCGCGATGG TGATCGCTGC GATCCCTCCG CTCTGGCTAC TGGCGTCGAC - 26221 CCACCTAACC GTGATGCTGG CGAAGCAGCA CTCGGAGCAC GCCGAAGTAC CTGTCTCGCG - 26281 GCCAGAACCC GCGCCTCGGG GCCTGGAGCC CGCTGCCGCT TGACTGCGCC CGACCGGGAC - 26341 AGAAATACAT AGAGAACCTA TGGATGTAGG AGGCACAAAA AAATACCCCC CGAGCCAGCC - 26401 CGAAGGCCAG CCCAGGGGGC ATGGTTCTGC TTCAGTAGAC CTTGCGAGTC CGACCCGAGT - 26461 TGATCATCGC CATGATGACC CAGACGGGCA ACCACATTCC GCAGGTGATG AGCGAAAGCA - 26521 ACAGGTGCAT CGCGTGGTTC GTCCTGACAG GCATGACAGT GGGCTGCGGC ATCGGAGGAG - 26581 GCGCGACCGG GTACGGCGAG CCCGCGTACC ACTGAGGTCG ATCTTGTTGG GGCGGATACT - 26641 GATTGGTCAT CCCGACAGCC TACTTGCCGA TGGGTCGCAT CAGCTCCTCG ACCGACTCGC - 26701 GCTCCACGCG GATCAGCCGG GGACCGAGCC GAACGGCCTT GAGCCGGCCG TCGGCGATGT - 26761 AGTTGCGGAC GGTCTTGGTG CTGACACCGA GGTAGTCAGC GGTCTCCTGG ATGGATGCTC - 26821 TCGGGGGCAT CAGCGCGGTC CTCCGTGCTT CATCGGTTGT CTCCCGAACC CTGGATCACG - 26881 CCACGATCCT TGCGGCTCTG GAGCTTGTTG AGGTTCCTCT GGGTGACGGT GCTCAACCAG - 26941 ACATCGAGCT GGTTGGCTAG CTGGGCGACG TACCACATCA CGTCTCCGAG TTCCGCCTGG - 27001 AGGTCGTCTC GGTTCTCCTG GGTGATGACA CCGTCTTTAT CCCGGAGGAT TTTCTTGACC - 27061 TTGTTGGCGA TCTCGCCGGC TTCGCCTACG AGACCCATCG TCACGTAGGA GAGACCCTCG - 27121 ATGCTGTCGC AGTCGCCTGC ACCGGGGTAG ATCGCTGTGT CGCTCGCGGC GATCTGGTAG - 27181 ATGTCGACGT GCATCAGATC ATCACCGGGA ACAACTGGCC ACCGGGCATC TGGATGAACA - 27241 CCGGGACGCT GGGGGTGTAG TCCGACGAAC CCGTGCCGCC CTCACAGGCG GACAGGCTCA - 27301 GGGTGGCGGC AAGGCCGATG ATGGCTGCTG CGATGGTCTT CTTCATCTGT TGCTCCAGTA - 27361 GCTAAGTTCG GACTCCAGTT CGCGGATACG CTCCTGTAGC CCTTGGTTTT CCAGGTACGC - 27421 CTCGGCGAGG TTGGCCTCGG CGCGGTCACG GGCCTCGTCC TTCGACGTGG CCTCATCGAT - 27481 TGCCTCGTGT AGCCGGCGGA TCAGATCTGG GATGGCACCG TGCAGACCGC ATATGAAGTC - 27541 GGCGTCTGCC TCGGAGAGGT GGGACGCCAC CAGATCCTTG TCCTGGGTCT CCTGGTTGAC - 27601 CGCCCAGATG ACGTGATCCT CTAGCCCGTG GTCGGTCTCG CAGATAGAAG GCGGTTCTAC - 27661 CTCCTCTGGC ATCCAGTAAG TCTTCTCAGC CCCGGTGGAC TTCGCCCACT GCTGGTAGAG - 27721 GATGTCGAAG AACTCGTGGT CCTGTTCGTC GGCGGTAATC ACAGATCGTC CTCTTCATCC - 27781 CATTCGTCGT AGTAACACGT ACAGCCGCAG CAGGTGCAGC AGCCGCACTC GTAGGTGCCG - 27841 TAGTCGTAGT CATCCCAGTC GTCTTCGTCC ATCTAGCTGT ACTCCTTCAT GATTCGGTCG - 27901 AACGCACGCG TCTGCACGCG CATCTCCAGG TCGACCGTTC GCTTCAACCA CGCCCATTCG - 27961 CCGTCGTGGT TGATCTCCCA CTGGCTCTTG AATGTCGCTG TCTCAACGAG GAACTCGACA - 28021 GTCAACGTGT GCAGTCCGTT GTTGCTGGGC TGGAATCCGA TACCGTCCTC AGCGATGTAC - 28081 CAGGGCAACT CCTGGCCGTC GAAGTAGACG GCCTTGTCGG TCACCAGTAC TTCAGGGAAG - 28141 GTGTGCTCGG TCAACGGCGT CCCAGGTATG GGATGACGCT GGCCCGGAAC TCAAGGAACA - 28201 CCATGTTGTC CGGGCAGTCC TCGGGGACGT TGTCGGGGCG TTCGGCGGTG TAGACGCCGA - 28261 TCTCGTTGCC CTCCAGGGTT CCAAGCTCGT TGAGCTTGTA GATCGCCAGA CCCATCAGCT - 28321 CTTCATCGAG ACCGTTCGGT GCTGGCAGTA CAACTTTGGC TTGTGGCATT AGCCCTCCCT - 28381 CGGAATTACG TATGCGCTGA ACTCGACGGC CGTAATGCCG TCTGGCAGTT GGAATCCGAA - 28441 CCGCTCTTCG AACTCCTCGT TGGTGATGGG GCCGTACTCG AAGGTTCCGG GCACTACCTC - 28501 GCCCTCCCCC TCGATCAGGA GGTACGCACC GGCGGCGTAC ACCTCCTCGT CGTTCGGCCA - 28561 TCCGACTACG GTCCCGAGGA CCGTGAACTT CCTCGGCTCC ATCAGGGCAC GTCCACTTCG - 28621 TTGATGAGGA ACCGCATCGG AGGTGGAGTG AGCATTGCCT CGGCTATGGC GATGAGGGCG - 28681 TTCAACTGAC CCTTCAGCAG CTTCTCCTCG TCGCCTGCGG GAAGGTGGCG CACTCGGCGC - 28741 TCCATCTCCT TGGCGCGTTC CAGATATTCG GTGGCTGTCA AGTTGTCCTC CTTAGTAATC - 28801 AGCGCCGTAG AGCGAACCCC ACGAACGCTT TCCGACCTCG GGGTCGGTGC CAACCAGCAC - 28861 CGGACCCATC TGTTCTTGCA TCAGGTGGCC AATGTGTGCA GCGGCTCTCT CAGCCTCTGA - 28921 GGCGGGCAGA GACGCGACGA TCTCGTCGTG GATAGGCAAC CGTAGGTACG GGGTGTATCC - 28981 GGCCTCGTGG AGGCGAATCA GAGCCCGACA GGTCACGTCC CGCGACGACG ACTGGATCAT - 29041 GTAGTTCAGC GCGGAGTATG TCCGCGAGCT GTCCACCGGC AGCCGCCGGC CCATCGCGTT - 29101 GACGATGTAG CCGTTGCGGC CAGCTTCCAT CGCCAGCTTC TTGCTCAGCC GCTCCACACC - 29161 GGGGTATGTC GCAGAGAACG CCTCATGAAC TCGCTTGGCC ACAGGGATCG AGATCCCCAC - 29221 TGCCTCAGCG AGAGCCTTCG CCCCACCGCC GTAGACCTTC TGAAAGTTGG CGGTCTTCCC - 29281 AACCTTTCGC GGCACCTGGG CTGCGTCAGC GGTCATCTGG TGGAGGTCCG CACCGTTCTC - 29341 GAATGCCTCG ATCATGTTGC GGTCGCCCGA CAGCGCCGCC AGGACGCGAA GCTCCTGCGC - 29401 CTGGTAGTCG ACTGAGGCCA TCACATCGCC TGGCTCAGCG ATGAAGCATC GCCGCACGAT - 29461 CCAGTCCGAC GACGGCAGCG TCTGCGCCGG GATGCCGGTG ATCGACATGC GCGAGGTCCG - 29521 CGCCTGCAGT GGGTTGATGA ACGTGTGGCA GCGGTCCTCA GAGTCCCTGG TGTCGATGAA - 29581 CTTCTGGACC CAGGTCTTCC GCCACTTCCC CAGCTTCTTA GCCTCCTGAG CGATGGCGGC - 29641 AAGCTCGTTG CCATCTTCGA CCAGCTTGTC GAGCAGAGCC GCGTTGACCT GGCGCTTGCC - 29701 AGTCTCGGTG CGACCGGTGA TCTTGACGCC CATCTCCTCA AGCCCCTCGG CCAGATCCTC - 29761 GGTCGAGTTG ACCTTCTCCA CGCCGTACTC GGTGAAAGCG ATTGCCTCCC AGACCTCCTG - 29821 ATCGGCCAAC CACTTCTCGG CGAGCGACCG CGAGTACTCC ACATCGAGCA GGAAGCCCTG - 29881 CCTGTCGATG TAGCTGCAGA TCTCACTGAT CTTGTGCTCG TACGGCACCA GCGACCGACT - 29941 CACGTCGGGC ACCAACGGTG TCAGGCTCTT GCAGACCCTC GCGGTGAAGA TCGTGTCCAT - 30001 CCCGGCGTAC AGCAGGTACT CCGGGTGGAA CAGGTCGATG GTCGACCAGA TCTTGGCCTT - 30061 GGTCGTCTTG TGCTCGGCGG CTAGCTTGGC CATGAGCTTC TTGACGTTCT CGGCCTGGTC - 30121 CTCGGAGATG AACTTCGCGA TCAGCTCTTC GAGCGAGTGC CCGAACCCGC CGGCCTCGAA - 30181 GGGCCGGGGG TCCACCAGCT TCGCCAGGAT CTGCGTGTCA AGCACGCGGG GCCACAGACC - 30241 CTCCATCTCG ATCCCGAAGC ACTGGTCGAG CACCTGGAGG TCGAAGGAGG CGTTCTGGAG - 30301 CACCATGCGC TTGAGAGCGC CGATGGCGAT CCGCACGTCC TCGATGAACA CGTCTCCCAG - 30361 CTCCACCGGC ACCACCCAGG CTTCGTCCTG AGTACCGAAC TGGACGAGGC GGCACTCGAA - 30421 GGTGTCGCTG TAGATGTCCA GCCCGGTGGT CTCAGTGTCG ACGGCGAGGC AGTTCAGGTG - 30481 AGCCCGGATG AAGTTGCGGA AGCCTTCCAG ATCCTCTGGG GTTTCAACGA CGTTGACGGT - 30541 GACGAGGTCT CCCTGAACCT CATGCCGCAG CTCGATCAAA ATGCTCTCCT ACTGGAAGTA - 30601 CTGAGGCGGA ATCCAGGTGG CTGAGGCCAT CTCCTTGATG GCCTGCTGCA TGGCCGCTTC - 30661 GAACGGACAG TCCGGGTCGA TGTCCGGCTT GTAATGGGTG ACGATGATCC GGCTGTTGCC - 30721 GCCGAAGTCG TGGCTGACCA AGCCCTTTGG GGGCAGCTTC TTCAGCGCCT TGATCAGTTC - 30781 CTCAACCGTG GTCCCGGTAG GGGCCTTGCC GTCAGGCAAT GCCTCCCCTC CGTACGGCAC - 30841 GTCCAATGGG ATCGTGTACC GCTCAACGTC TTTGATCTTC ATCGAGCCTC TTCCTCTTCG - 30901 ACTACCTCGT CTACCCGGCG GAATAACTCC GCTAGTTCTG CGGGTAGCAA TACTGGGTAC - 30961 TTCTCTCGGG CTTCCTGCAT CGCTACCGCG ATCCCAATCA GGGCAGCGAG CAGTTCATTG - 31021 ACGGAGTACG CCAACAGCTC TTCGCGGATC TCTTCTCGGG TCATTAGTGG TAGATCCCCC - 31081 GGACGGTGCG CGAGATCGTG GCAGGGTTCA CGCCGTAGTT CTCGGCGAGA TCCTTCTGCT - 31141 TCATACCGCC CAGGTACGCC TGGCGGATGT CCTTGACCTC GCGCTCGGTG AGCTTCTTGC - 31201 GGTTCGGCCG GCTCGGGCCG GTCTCAGGCT TGACCTGAGC CAGCGCCTTG CCGAACAGCT - 31261 CGTTCTGCGT CCGCTGCTTG ATCGCGTACC GACGGTTCGC TGCAAGCACC TCGTTGAGCC - 31321 GCTGGGACAA CTTGACATTG GCCTCACGCA CTACCTCGAC CTCTCCGAGC AAGTTCGTGA - 31381 TCCGGTAGTC CTTGTCCTGG TTCTCGATGG CCAACCGGTT GTTCTCCTCG GAAAGCATCG - 31441 AGACCTTGTA TTGCGCCTCT CCCAGCGCAG CTTTCAGGTG CTTCTTCCTC ATTCAGCGCC - 31501 CCTCTCTCGG CGGAACTGTT CGTACTCGTC TTCGGTCATG TAGTAGTAGT AGTCAACGAC - 31561 CTTGTCCCAG TTGAAGGTTC GGGACGTGCC GTCATCGAAC GCGATGATCA GGACACCCTC - 31621 TTGGGTGTCT AGGATCGGCT CGCCAGCCAC GACGTGGAAG CGGTCCTCGA GGGTCACCGC - 31681 AGTCGCTCTG CGTGCCATGT CAGTTCCTCT CAGTAGCTGT AGGGGACATC CGGGATGTCC - 31741 TGGTAGGTGT TGGGTGCGAT CTGTCGGAGC TGCCGAAGCA ATTCCCCTGC CAGCTCACGG - 31801 ATCTCGGCAT CCGCGGCCTC GTGCCAGCGG GCCTTGATGA CGTACCGCCA CGCCCGATGG - 31861 TTGCCCGTGA CGACCATCGG TGAGTTCGTC ATGTTCGGCA GGACAGCTCG CGCTGCCTCG - 31921 CGGGCCTGCT TGCGCGGCAA GCCCCGGTCA GCCAGCCGGT TGACGATGTG TTCGTAGACA - 31981 GCGTCAATCT CAGAGCTGAC GGACTCCATG ATGTGGACGA GGTCGTCTCG GTCGTCGGGG - 32041 TGGAGCTTGA ACAGAGCCGG GGGCAGATGG ATGCCAAGGT CGGTCGGATC CACATATCGC - 32101 TGAGACACCA CCGAGAAGCT CAAGTGACGG TGACGCTCCA GCTCGGTCAG CACCGACCTG - 32161 CTGGCCTCGA TGTAGAACGT CGCCGAGGCG TGCTCGAACA CGCTCTCGTG GCCCAGATCG - 32221 ATGATGTGGT TGAGGTAGTC CTCGTTCTCG GCAGTTGCCG GGTTCGGTCG GTGGAACGAC - 32281 CGGTAGCAGT TCCGGCCCGC GAACTCGGCC AGCTCGTCGG CATCGAAGTC GCCGAAGTAG - 32341 GGATCTTCGT CCTTGGATTC TTCGAAGTCA TCGACCTCGA ATCCGATGTC CCGCAACGCA - 32401 CCCGGATCGA TCTCGGTGGC AGCGATCAGT TTGGCTTTCA TACTCTCCGC TCAGAGTTGG - 32461 TGGAACGAGG TCAGCCAGGG GGCAGCGAAG CCCTTCTACA GCTCCCCTTG GCTCGTTACC - 32521 GGCTTCTCGA CCTCGGTGGA TGTCAAGTAG TCGAGATGAC TACTTCTTGT CGGGCCATTG - 32581 CGCGTCACAC TGCTGATCGC GAGGTGCGGT GCAGGAGAAC AGCGCGTACG GCTTGCCCGT - 32641 CTTCTTCGAG ACGCCCGACT TGTAGACCAT CTCGCCGTGC TGGCAGTACC GCTTCTCGCC - 32701 ACCAGGCGCT TCCTGAGCTG CCTGCGGGGC GCGAGACTGC TGCTGGCCAC CGCCGCCGCC - 32761 GTTGGCCGGC GCGGATCCAC CGGAGCCTGC GTAGTGGCCT GCGATCTGCT GGACCTTGTC - 32821 CATCAGCGCC TTGAACTCGG CGGTGTTGAC CTTGGCCAGC ACGTCGGCCG GGTCCGCACC - 32881 CTTCACGACC ACCCACGGGT CGCTGTACTG ACCGGCGAAC TTGAACGTGG CCGACACCCC - 32941 ATCGGTGGAG TGCTGGACCG CCATCGAGTC GCGCACAGCA GCCGAGGCCG TCGTCACCGT - 33001 CGCCGACGGC GCGGTCTCAG GCTCAGGAGC CGGGGCCGGC TCGGGCTGGG CAGGGGCGGT - 33061 GCTCCACGGA TCGTCGTAGG ACAACTGGTT ACCTTTCACT TAATGGGGCA TGCGCCGTTG - 33121 GCGCACTCTT CATCGACACC GTCTTCGACG GCTTTGGCCG CAGCAGATTC GTACTGCTGC - 33181 TTGGTGATTC GCTCGTACGG AGCCTGCGGG AAGCTGGACT CCGGGAAGAT CGTGGAGCCC - 33241 TTGATGAGCC CCGCGAACCT CTTGAGATCG GCTGCGACAT CCTCGGCCTC GTAGGCGTCT - 33301 GGATGGACGT TGGCGGTGAA CGACACCGCG TTGTCAGCCC AGCACATCTG GTAGAGCGCC - 33361 TGGAACGCCA GGAGCTGGTG GAGGGTCAAC TCGTCGGCTG ACTCAACGAT CTCCTCGTCC - 33421 CAACCGAGTT CCTCGACAGC CTGGACCAAC GTGTCCTTGG TCGGGATCGA AACCACCTCG - 33481 GTGTTCGGAG CGAAGAGATC CTTCTCGATC TCGTAACCCT CGGCTGCCAA CCTCCGCAGC - 33541 TCGGCCATGT CGCTGTTGAG GTTGAACCGC ACACGCCGGA TGAAGTACCG CGAGAAGATC - 33601 GGGTGGATCC CCTCGGAGAC TCCTGGCATC TTCGCCACCG TGCCTGTGGG AGCGATGGTT - 33661 CGCTTCTTCA CCGGGACAGG GATCCTCAGA TCATGGGCGA ACCGTTCGGC CTCTGAGTCG - 33721 ACCTCAGCGG CCATCTCCCG CAAGAACTGG GTGAACCGCT TATCTCCGGG TGCCTCGGAG - 33781 TACCTGCTAC CTGTGAGGGC CAAATAGGAG GCAACTCCGA GATGACCCAC GCCGATGCGA - 33841 CGGTTTCGGT CCAGAACCTC CCGGCTCTTC GGGTCGGCCA CTTCCGAGAA CGTCGCCCGG - 33901 ATCAGGAATC TCGTCATCAG ACGATGCGCC CGGATCAGGT CGAGGTAGTC GGTCTTGCCG - 33961 GCCGGCGTCA CGAACGCCGC CAGGTTGATG TGGCCGAGGT TGCACGGCTC CCACGGTTCG - 34021 AGAGTGATCT CGCCGCATGG GTTGGTGCAG ACCACCCGGT TGGGCTCACC GACGTTGGAC - 34081 AGTGACGAGT CCCACATCCC CGGCTCTCCG TTGCGTACGG CTCCCTCGGA GAGTGCCTTG - 34141 AGCACTCGGT GGGCTCGCTT CTGCTTGGGC ATGTCCTCGC GGGCGACCGC GAAGCTGCCG - 34201 TAGCCCTCCT TGGCCAGACG CCAGAACTCG TCGTCAACCT CGACCGAGAT GTTCGTCGTC - 34261 CAGTGCTCGC CCGTGCTCGC CTTGATGTTG ATGAACTTGT CGATCTGGTA GTCGTCCCAG - 34321 TGCATCATCG ACATCCGCGC CGACCGGCGC ACACCGCCGG CCACAACACA CTGAGCGATG - 34381 GCGTGGTCGA CCTCCATCGC GGCGATGCCG TCGAGCGTGA TCCCTGCGTA CTCCGAGAAG - 34441 ATGTTGGCGA CCTTCTGCAG CATCACAGCG AACGGCAGCG GGCCGCTGGC CACTCCACCG - 34501 AACGTCTTGA GCTTGGCCCC TTGCGGCCGG ATGCGGCTCA CGTCGTACAC CCGCTGGTAG - 34561 TGGACCGTGC CGGGTCGGTA GTGCGTGTCG ATCAGATCGA CCAGCGCAGC AGCCCAGCCC - 34621 TCTCGTGAGT CCTCGATGGC GTAGGCACCG GCCCAGTCGT GGCTGTAGTG CTCCGACAGA - 34681 ATGCCTACAT CCTTCATCGC CTGGTAGTCG ACATGCTCTG GATCACAGAC GATCTCGACC - 34741 CGCAGGGGGT TTACGACCTC GGGGTAGCCT TCGAGGTAGT GGTTCGAGTA GTTCGCCCCG - 34801 ACTCCCCCGC CCTCCATCAG GCGCATGAAC GTGAACTGGA AGTGGTCCGA GATCTTCTCG - 34861 GGCCAGCCAG CTACCCAGCA GTTGAAGAGG TGCTGCGCGT TCTTGACCCC CGAGGCCCAC - 34921 AGATGCCGAC CTGCCGGCAG CACCTTGAAC TTGGTCATCA GACGAACGAG ATCTTCTCGC - 34981 TCTCCTTCCA ACATATGTCG CCGGTCGACA AGAGCAAGAT TGCCGTCCAC GACCCTCTCG - 35041 ACCGTTTCCG GCCAGGTTTC CTTCGAGCCG TCAGGCTTGG TCCTGGCGTA GGTTCGGTTG - 35101 TAAACGAGTT CACCGGTTGG TCCCCAAGGG ATTTCGTCAG TCAACTACTT CCTCTCAGTC - 35161 AGTTCGTATC GCTTGAAATA GGCGTCGGCA GAGTCGCCGC CAGAGAACGA GACCCCGTAC - 35221 TCGACCGGGC CTGCACCACG CACCTCGCAG GTAACGACGC CCTTCCTTCC CCGGAACATC - 35281 GGCCAGGTTC CCTTGGAGGG GTGCTTGGTC TCGTCCCGCT GGACGATGAC CTTGGTGCCC - 35341 TTCTTCATGC CGACTTCCGT TCTCCGTAGC CGGGAGTGAA GCAACCCCCG ACGTACAGCT - 35401 CGAGATCTTC TTGCGACCAG TTCTCCAGTC GCATCGGCGG CTGGTGCGGG AACAGCTCCG - 35461 GGAACACCTC GGCCCGGTAC AGCTCCGAAC CGGGCATCCC GTTGAACGTC GGATCAAGAA - 35521 TGTTGTGCAT GGCACCTCCC TCCCAAGAAC TCGGAGATCG GCGGCTCGTA GAGGTAGCCA - 35581 TCGCGCAGCT CGGGGTTCTC GATGAGCATG ATCGCGATGT TCGCTGTGGG GTCAGAGTGC - 35641 CCATCCCCCT GCGACTTTCG GATGTCTGGG AAGATAGCGT GCTTGCTGCC CGGACCATCC - 35701 TTGACGATGA CCTTGCCCTT GTCGTCCTTC TCCACGCCAG CCGTGATCGC GATGATGTTG - 35761 ACGTGCTCGG TCAGCGACTT GTGAGCGCGG AACAACCGGT TCTGCCCGCT CTTATCCTTC - 35821 GGGGAGATCC CGTCGGTGTA GCGGCTCCTG ATCGCCTCTG CATAGCCCCC GTTCTGAGCG - 35881 TCCAGAGCCT TCATCGCCAG CGGGAGGATG TCGACCAGGT ACCGATTGGT CGACTCCCCC - 35941 TGCAGAGCCT CTTTGACGTT CTCGGACGAG TAGTGGCTGC GCTCCTGGAA CAAGTCGCGG - 36001 GCCTTGGCCG CTCCCGACAG GATGTTGCGA ACCTGATTGC GTACGTAGTG AACTGCCTCA - 36061 CCACGGTGCA AGCTCTCCAG CGTCTTCTGG ATGTACGGGC TCTCGAGGTA CCAGACCCAC - 36121 AGCTCTTGGA TGATCTCCTC GGCTGTCAGG TTGGTCTCCC AACCGATCAG CGCCTTCCGG - 36181 GTGGCCCTGC TGAACAGCTT GCTGATGTCG TCGGTCAAGG CATCACCTTT CGTAGGTACT - 36241 CCTCCCGGTC CAATCGGCGG TCGAGGTGTC GAGTGACCTC CTCCGCGAAG ACCTCGCGGA - 36301 CTTCGCTGGA GGTGATCTGG CGCGAACGTG CGTTCTTGTG CAGGTACGGC AGCTTGGTGG - 36361 CTGTCAAGTT CTAGACCTCC CAGACTCGGC CGTCGACCGA GAACCGGCCT CCGACAATCG - 36421 GAACAAGCTC AGGCTTGACG TGCTGGCCGT CGACCGTCAG CAGAGCAAAA CCACTCTGCC - 36481 AGTTGGCTGT TGCACCCTTG AGGTACTGAG CTAGCTTCAT GTTCATCAGG TTGCCGACCT - 36541 CCATCGACCA CAGCACCTTC TGGTTGCCGC CGTAGCCCAG CGTGTGTGGC TTGATGCCCT - 36601 GGCGGTGGGT GTGTCCGATG ATCACCGACG TGCCGAACCG CATCATCGCG TTGTACGCGG - 36661 TGTCAGCGGA CTTCTGCGTC ACCCGGACCC CACCACGGTG GCCGTGGGTG GAGATCCAGC - 36721 CTGGAGCGAT CTTGTAGAAC TCAGGCAGCA CGTCAACACC GAACCCGTCG AAGTCCAGCA - 36781 GGTTCTGGAA CTGGAACGAG CTGACGTACT CGACCAGCGC CGGGGCGAAC TGGTGCAGGT - 36841 AGTCGACTGG CCGGCGGTCG TGGTTGCCCT CGTGGACACC AACCGGGCCG TCGTAGACCT - 36901 GGCGCAGCGG CTCCAGGAAC CGCCGCTTGC ACTGCTCGGA GTCGGGCTTG ATCCGCTGAG - 36961 CGAACTCTTC CTTGGTGCCC TTGGTCCACC GAGACGGGCT CGGGTAGTCC ATCAGGTCAC - 37021 CGATGTGGAC GACCTCGTCA GGCTGGGTGT CCCCGATGTA GCCGATGACC GCCTTCAACT - 37081 GCTTGCGATC ATCGAACGGA ATCTGGGTGT CCGAGATGAC GACGATGCGC TTGCTCACTC - 37141 AGCGACCTCG GTGAAGGGGC CCCGCATACG TTCCTCGTGG GAGCTGGCGT TGCCTCCTGA - 37201 CCAGCGTCGC TTGCCCACCT TGGTGTGGTG CAACCCGTTG GGGTAGTAGA TCCACTTCAC - 37261 TCCTGTGGCG TTGGTGACGG TCTTCACATC GGCAGGAACG TCCAGCAAGG TGTCCCACTG - 37321 GCGAGGCCCC TTGGGATACC GCTCGTCCTC GGGGAGCTGC ATCTTCTCCA GAACGCCTGC - 37381 GTAACCGGCG ATGTCGACCA CCGTGTCCTG GTGGTAGCCG TTCTCCATGA ACCGGGCGAT - 37441 CTTCAGCAGG ATCATCATGA CGGCCACGTC CTCCGGGGTG AACTCGACGC CGCGCTTGTA - 37501 CGCGCCCCAC AGGGTCGCGA TGCGTTCGTG GTTCTCCTTG GCGTCCCCGT AGTCCTGGGC - 37561 TCGCTGTCCG TTGATGATCT CTTCGGCGGT GGTCAGAATG CTCACAGTCC AGTCTCCGAT - 37621 GCGGTGTAGT AGTCGATCAG CTCATCGAGC TGGTCCGGTT GATAGCCGAG GATCGGCTTG - 37681 TGGGTGTCAG TGACGACGAC GGGAACCGAC ATCGCGTTGA GCACCTTGGT GACGTAGTCG - 37741 TACGCCTCCG AGTTGGCCGT GACATCGACT GCGTCGAAGT CGATCCCGGC AGCCGTCAGC - 37801 TTGTCTTTGA CTCGCTCGCA TGGCTTGCAG CCGGGACGGG TGTACACCGT GACCGGCGCG - 37861 AACAGCGTTC TCACGTGAGC ACCATCCCAG TCGATGTATC GGTCTCCATA CATCAGATCC - 37921 TTTCCAGCAG AGCAGCTTTG CCCTGCGATG TGACTAGTGA GTTGACATCC TCGCCTTCTG - 37981 GCATCGGGAT GATTCGGGCG TTCGGCAGCG TCTTCGCCAC CGACCGGGCG AACTCCATAC - 38041 CGGCGTCGTC GCCGTCGGCC AGGATGTTCA CGTTGCGGTA GCCCAGGAAC AGCTCTCGGA - 38101 AGTACGGCTT CCACTTCTGG GCTCCGCTGA GCCCCACCGT CGGCAGCCCA CACAGCTCGG - 38161 CGGTGATCGT GTCGAGTTCT CCCTCGCAGA TCGCCATGTC CTTGCTGTAT TTGGTCAGCG - 38221 CGTAGGTGTT GTAGAGCCGG TCCTTCTCCC CTGGCATCGA CAGGTACTTC GGTGTGCCAC - 38281 CGTCGATTCG GCGATACCGG ATCGCAGCTA CCGTCCAGTG ACGCCAGGGC GACCACCGCA - 38341 TATACGGAAT CGCCAGGCAG CCCCGGTACA TCTCATGTCC AGGGAGTGGG TCGTCCACGA - 38401 ATCCCAGACC GAACCGGCTT AGTTCCGCTC GGCCGGCCAG CCCGCGACTC GCCAAATACT - 38461 CGTCGGCTGG GCTTCCGGGC AGGCTTTCTC TGTACCGGGA CGTTGCCTCC CACAGATAGG - 38521 TTCTCTGCGA TTCGCTTAGC CTCTGCAAAT GTCACCTCCT CTTCGTGACG AATGATCGAG - 38581 ATCACGTCTC CACGGACCCC GCAGGCCATG CAGTTGTAGC CCTGTAGGTC GTAACTGACT - 38641 GCGGCAGACG GCGTTTCGTC GCCGTGGAAG GGGCACAGGC ACTTGTTCCA CTCGTGGTGG - 38701 TCAGGTGGTG GTTCCCAATC CGGGTGGTAG CGAAGAATCG CCCTCGCGAT GGGCGAGTCG - 38761 TTCATTCGTC CTCGTCAAGC TCCTCGGGAG AGAGCCCTTC GAAGATCCCG TTCAGGACGG - 38821 CGGCGAAGCC CTCGCCGGTC TCCGCTGCGT CGAGCATCTC TGCAATCGTC TTTGCCATGT - 38881 TTCCTCCTGG TGGATGTCAA GTTCGAGACA GCTTGTCAGC CTCGACTGGA GCGATGCGCT - 38941 CCCCGATGAC TTGGACGGCC GGCGGGTTCA GCAGGTACTC GATGGCCCGT TTGAAGAACT - 39001 CGATGCAGTC CCTCGCCCAG CCCAGCGTGT ACTTGTTGCA CATCGTGCAG AGCAACCCTC - 39061 GGACGATGCC TGTCTTGTGA TCGTGGTCGA CCGACAGGCG CTTCTTCTTA CCGTTGGCTC - 39121 GCTGGCAGAT GTAGCACCGA CCACCTTGGA ACTCGTAGAT CTGCCAATAC TCATCGCCGG - 39181 TGATGCCGTA GGTGGCCAGG ATCCGGGTCT CCCAGCTCGT AGAGCTGCGA GCCGTCCTGA - 39241 ACTCTCGGTG ATGAGTAGCG CATCGTGGCC CTGGATACTT GGCGTCTCGC GTGAGCGGGA - 39301 GCCCCTGTGC GACACAGTCT TTGCAAGGCT TCCGCTTGTG CTTACGGTTC TGCACCCGGT - 39361 ACCCCGGAGA CCTCTTCGCC GCCCTCGGCA CGCGCGTCCT CCTCCCGGTT CTCCATCACC - 39421 ATGCAGAACC ACGACAGCAG CCCTGCCAGG GAGATGTAGA AGGCCACCAG AACTTGGCCG - 39481 CTCACTTCAC CATTCCTCGA ACCCACCAGC GAGACAGCGC CTTACGCCCT TTGTCGAGCG - 39541 GGGTCAGCTC GCGCTCATCG TCCTCACCGA AGTCGAACTC GATGCTGGCG ATCTCGTAGC - 39601 CGAGGATCTT GAACGACACG TTCATAGGCG GTCTCCGAAG TTGATGACGG GAATGCCGGC - 39661 CCTTTCGGCC TCTCGCATGC AGTGCCGGGT GCCGACTGAG TTGCCGAGGG GGAACGCCAG - 39721 ACAGATGTCC GCACCGGCCC TGACCATCTC GATGTTGCGG AGGATGCCAG CCCGCTTGCC - 39781 GTAGCGTTCC CAGTCGGCTC GGTGCAGCTC GGGGAGCACG TCCCATCCCT CCTGCTTCAT - 39841 CCCCCAGGCC CAGCGGTCTG CGATGTCGTC AGCGCCGCGA GCGCCGCCGT GGACGACCGT - 39901 GAGACCGGAG AAGGACCGGT GGTACTCAGT GGCCAACGCT TCCCAGACCG TGGTGCGGTC - 39961 CTTCCAGATC CGAGATCCGG TGATCAGTAC TCGCCGCATC AGATCGCCTC CCACTGCAGG - 40021 CCGTCGTGCG ACGTGACCAG CTCCGCTTCG TAGACGCCGT AGCGGGTGGC CAGGAACTGG - 40081 ATCATCTGCG CCTGCTTGTA CCCGAAGGGA CATTCGTGGA CGCCGCTGAT CGGGTATCTG - 40141 ACTCCGTATT TCACTTGATC CACCGCTTCG CGATTCGGTC GACGTTCTCC TCGGAGACGT - 40201 TGCGGGCGAG GCCGGTGAAC TCCTGGCCGT GGACCTTGGT CTCGATCACG CGAGGCTTGC - 40261 GGGGATCCGG GCTCTCCGGG TCGATCCGCT TGTGGGTCCA GACGGTCGGC TTCGTCTTGA - 40321 TCAGAGCGCC CAGCACCTGC TGGCGCAGTG GGTTGGTCTT GCGGGGCATA GCGTTTGGAG - 40381 TGGTCATCTG GATCCTTTCC TCGGTGGCTG TCAAGTCGGT GTGCGTAGTG AAGCCCCCCC - 40441 AGGCATGCGC GCCCCGCCTG GGGAGAGTTG ATCAGCGCAG TTCGATGTCG GGCAGGATCG - 40501 CCTGCGGCTT GAAGTTGACC TGGTAGAAGT CGGTCGAGAC GTTTGCGCCA TCGACCTGCT - 40561 CCATGAAGTA GGAGACGTTG TCCGACAGGC CCAGGAAGTG CTTCTTGATC CCGTCCTTGG - 40621 TCTTGCAGGT CACGTCGAGC TTCTTCGACG CGGTGTCCGC GTTGATTGAG CACCGGCCCT - 40681 GGATCTCGAG CAGGTACTTG TCCGTGATCC CGTTGAAGAA CACGATCCGG CGATTGATCT - 40741 CGAAGTTGTC AGCGGCCTTG CTGACGTTCT CCGATGCGAC GTCGGCGTCG GAGGTACACG - 40801 CGGAGAGGCC CAGGATCGCC GATCCGGCGA TGAGTGCGGT GGCGATGATC TTCTTCATGT - 40861 TCGCTACTTT CTGTTTGGTG GATGTCAAGT TAGTGACCGA AGTCGTTGAT CTGCATAGTG - 40921 TCTCCGACGA ACTCCAAGGA AGCGAAGTCT TGTCCCGACG GGTCCGACTT CCCCCCTCGG - 40981 TTCTTGACCG TGGAGACGTT GAGCATGTCC GGGCCGAACC CGTCCGATAC TCGGTGGAGA - 41041 GTGAGGATCA TCTCAGGAAC ACGCCCGATC TGACCTTTGA TGCCCGACAA CGGGATCGGC - 41101 TTGTCGCCGT CGTTGTGCGG GCCGGTGACG TGGTGGAGCC CGACGACGCA TGAGCCTGTC - 41161 TCACGGCCCA TCTCGTGTAG GTAGTCCATC AGCGACTCCA GACCCGAGAA CGGGTCGTCT - 41221 CCCTCGCTTG AATCGGTGCG GACGTTGGTG ATGTTGTCCA CGACGATCAA CGCTGGGAAG - 41281 TCCTCGTACA GCGCGTCATA CGCGGCCAGA GCGTTCTCGA TCTCGTCCAA CGACGGTGAT - 41341 GCCTTGTAGT TGAACCGGAT CGGGATCTCG TCTAGTGAGT CAGCTACCGC GTCCTCGATG - 41401 TTCTGCTCGC GAACAGCCCG CGTAGCTCGT TCGAGCGACC ATCCGCTGAG GATGGACACC - 41461 GAACGGGAGA GCTGGGTGAA CGCATCAGAG TCGGCCGAGA AGTACAACGT CGGCACCTTC - 41521 GACTTGAGCG CGTAGGCGAG GACGAACGCC GACTTCCCGG TGCCGGGGCC GGCGCAGACC - 41581 AGGACTAGCT GGCCTCGTCG GAGATGTGTA CCTTTCTGGT CAAGCGCGGC CCAGACCGGG - 41641 GGTAGCGGAT CCCCCGCCGA CCCTCGGATG TAGAGCGATT GTCTAGGTGT GTACACCTTC - 41701 CTCCTCGTGG ATGTGATTGA CCAGGTCATA GATCTCGTCG CGAGAGACCA GCCGGCCCCA - 41761 GGCGTCGATC CCCACGTGGA TCTGTCTCCG GTGGATGTGT CGGGACAGGA TCATCGGCGA - 41821 ATGCGTGTGC CCGTGGATCA GGATCTTGCC ATCGTCACGG AGCCTCCACT GGGTGTGTCG - 41881 GTCCTCGCTG GTGTGGTCCC CGACGTATGG GAAGTGGCTC AGCAGAACAT CTGTGTGCCC - 41941 GCCAGCGTCC CCGTACAGCG GCACCCGGAT ACGAGCTGCC GTCGACACAT GCTCGAACAC - 42001 CATCCAGTAC GCACCAACCA GCTTGTGAGC ATCGCGGTTC ATCGGGTGGG GCCCATCGTG - 42061 GTTGCCCAGG ATCAGCCGTT TGCGGCCTGG CCGATCCGAG ATCCACCCGA GGGCATGTAT - 42121 CTGCCCCTTG GTGGAGCCAG AGGAGATGTC ACCTAGGATC CAGACCGTGT CGTCCTTGCC - 42181 GACGACCGAG TCCCACGCCT TCGCCAGGGT GGCGTCGTGC TCTTCGACAT CATCCGCCAG - 42241 GTTGCGGATC TCCATCAGCC GCTTGTGTCC GATGTGTAGA TCGGACGTGA ACCAGGTGTT - 42301 GCTCATGGCT TCCTTTCAGA ACGGCGGGCC GTACAGCTCG ATCACCAGCG CGTGCAGCTC - 42361 CTCTGCCGCG TCGTCACGCT CGAATCCGCA GCAGGAATCG TGCCGGTCGA GGATTGCGAC - 42421 GATCTGGTCG TAGAGGCTGG GCCTCACTTC ACCTTCTTCG GATCGATCAA GGCGTCGTGA - 42481 ATCGGCCGAC CGGCGCGAGC CGCGTGCGTC TCGGCGTCCA AGGCTCGCTG CATCTGGTTC - 42541 ATCAGCCGGG TGCCGCGCAG CTTGAGGATC TTCATGGTCG CCCGACCCTT GTATCCAGCG - 42601 CGGTGCATCC GTAGGACGCA GGCTGTCTCG TGCGGGGCTA TAGGTGACCT CAGCGACGGG - 42661 TGGTTTGGAT CCCAGTTCGT CATGTCTTCC TCTCGGTGGC TGTCAAGTTG GTCACAGACC - 42721 GAACTCTTCC TGGTACTGCG GGATGAAGTG GCCGGCCGTT CATGTTCGGC TCGATACCTC - 42781 TCGCGTCACG AACTCCTGCC CGTTCCATCT CCGACCGTCC TCGAACTCGA TCACGATCTC - 42841 TCGTCCGGGA TGACGCACGG CCTCCGCTTG GGCAAACCTG CGTGCAGCCT CTGGGGTCGG - 42901 GAACGGAAAC TTCTGCGAGG CGTACAGCTC CTGGTGCCAC TTCGGCTTGT CAGGAATCGG - 42961 CCCCATTTCC ACGTACGTGT AACCCGCGTC GGGGTCGAGT TCGAGCGTTT TCTTGTATTC - 43021 CTTCGTGCCT GCCTTAGAGG GAAGGTGAGT ATCGGTGGCT GTCAAGGTGA CCTCACTTAA - 43081 AAACAGGGCA GCTGTAATTC ACATCACAGA AGCCGCATTT GTCAGGTTCA GGCAGAGGCT - 43141 CGAAGTCACC AGCCTGGATC CGAGCCTCGA CCTCATGGAA CCTCTCGGTG ATCCGCTCCC - 43201 GCGTCCAATC GGTCAGGTCG TAGGGCGCAG TGGGCTTCGC CTTGATGCCC TTCTTCCCCG - 43261 CCATGAAGTA GTCGCCCGTC TTCGGAGCCT CCACGTCATA GGTCATCGCG ACCGCGAGCG - 43321 CGTACACGCC GAGCTGGAAG TCGTCACCCG GCGAGTTGCC GGTCTTGTAG TCCCGGACTC - 43381 GAAGCTCACC GTTGACCACG ACGACCGCGT CGATGAACCC TCGGACGCGG ATGCCGTCCA - 43441 GCTCGATGTT GAACGGAAGC TCGATGGCCG GCTTGGGCTG TTCACACTCC TTGCAGTTGG - 43501 TGTCTTTCCA CGCCTCCGTA GAGCAGATCC CTCGCCCAGG GGTAGTCCAG ATCTGCTGGC - 43561 CCTTGTCCTT CCGCCACGCG ATGAACTTCT CTACCTGCTC CAGTCCAAGG TGGAACCGGC - 43621 GCTCGATGTC ACGCTCACCG TTGTACGGCC CGGACCAAAA CCACCACTCG AAGTTCGGGG - 43681 TTTCGTCGCA CAGTGCTCCG ATGTCCTTGG CGTACTCCTC GCGGAAGATC TCTTGTGCCC - 43741 GTTCGAGGCT CATCTCGCGG CCCTCGGCCA GAGCCTTCTC GTAGACCTCA GCGACGGTGT - 43801 GAAACGCGGT GCCCTGCGGC AACCACGCCG CAGGACGAGC CCATACCTTG TCGATGCGAG - 43861 CCAGCTTGTA CGCCTGCGGG CAACGTGTGT ATTGGTTCAA CTGGCTGACG CTTCGCAGCG - 43921 GCAGCAATGT CTTGGTGTCT GTCACGCAGC GGCCATCCTT CCCTTGCCTA TCGTCTCGTT - 43981 CAGCGCCCCG TCGACAGCGA CACTGAGCAG TTTTGCGACC TCCGACATGT CAATCGGATC - 44041 CTTGGGGAAT TGGTCAGCCT GAGTCATCCT GAGCACCATC CACTCGGTGC CCTTGTCGCA - 44101 GTGGATCATG GTCGGATCCT TAATTAAGAT CCTTTAGTGA GGGTTAATTG CGGCCGCGAA - 44161 TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT - 44221 AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG - 44281 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT - 44341 GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT - 44401 TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT - 44461 AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG - 44521 CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA - 44581 AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG - 44641 CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT - 44701 TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC - 44761 TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA - 44821 CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT - 44881 ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT - 44941 ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC - 45001 GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA - 45061 TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG - 45121 TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG - 45181 AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA - 45241 AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA - 45301 GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA - 45361 CTGAGCGTCA GACCCCTTAA TAAGATGATC TTCTTGAGAT CGTTTTGGTC TGCGCGTAAT - 45421 CTCTTGCTCT GAAAACGAAA AAACCGCCTT GCAGGGCGGT TTTTCGAAGG TTCTCTGAGC - 45481 TACCAACTCT TTGAACCGAG GTAACTGGCT TGGAGGAGCG CAGTCACCAA AACTTGTCCT - 45541 TTCAGTTTAG CCTTAACCGG CGCATGACTT CAAGACTAAC TCCTCTAAAT CAATTACCAG - 45601 TGGCTGCTGC CAGTGGTGCT TTTGCATGTC TTTCCGGGTT GGACTCAAGA CGATAGTTAC - 45661 CGGATAAGGC GCAGCGGTCG GACTGAACGG GGGGTTCGTG CATACAGTCC AGCTTGGAGC - 45721 GAACTGCCTA CCCGGAACTG AGTGTCAGGC GTGGAATGAG ACAAACGCGG CCATAACAGC - 45781 GGAATGACAC CGGTAAACCG AAAGGCAGGA ACAGGAGAGC GCACGAGGGA GCCGCCAGGG - 45841 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCACTGATT TGAGCGTCAG - 45901 ATTTCGTGAT GCTTGTCAGG GGGGCGGAGC CTATGGAAAA ACGGCTTTGC CGCGGCCCTC - 45961 TCACTTCCCT GTTAAGTATC TTCCTGGCAT CTTCCAGGAA ATCTCCGCCC CGTTCGTAAG - 46021 CCATTTCCGC TCGCCGCAGT CGAACGACCG AGCGTAGCGA GTCAGTGAGC GAGGAAGCGG - 46081 AATATATCCT GTATCACATA TTCTGCTGAC GCACCGGTGC AGCCTTTTTT CTCCTGCCAC - 46141 ATGAAGCACT TCACTGACAC CCTCATCAGT GCCAACATAG TAAGCCAGTA TACACTCCGC - 46201 TAGCGCTGAG GTCTGCCTCG TGAAGAAGGT GTTGCTGACT CATACCAGGC CTGAATCGCC - 46261 CCATCATCCA GCCAGAAAGT GAGGGAGCCA CGGTTGATGA GAGCTTTGTT GTAGGTGGAC - 46321 CAGTTGGTGA TTTTGAACTT TTGCTTTGCC ACGGAACGGT CTGCGTTGTC GGGAAGATGC - 46381 GTGATCTGAT CCTTCAACTC AGCAAAAGTT CGATTTATTC AACAAAGCCA CCGAACGCCA - 46441 GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA - 46501 AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGGAAG ATTCCGAATA - 46561 CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GGGGTCCTCG CCGAAAATGA - 46621 CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG - 46681 CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA - 46741 AGGGCATCGG TCGAGGAACT TTCGGCGGCT TTGCTGTGCG ACAGGCTCAC GTCTAAAAGG - 46801 AAATAAATCA TGGGTCATAA AAATTATCAC GTTGTCGGCG CGGCGACGGA TGTTCTGTAT - 46861 GCGCTGTTTT CCGTTGGCCG TTGCTGTCTG GTGATCTGCC TTCTAAATCT GCACAGCCGA - 46921 ATTGCGCGAG CGGGGTTTTG CTGAAACCGA CACACAGCAA CTGAATACCA GAAAGAAAAT - 46981 CACTTTGCCT TTCTGACATC AGAAGGGCAG AAATTTGCCG TTGAACACCT GGTCAATACG - 47041 CGTTTTGGTG AGCAGCAATA TTGCGCTTCG ATGAGCCTTG GCGTTGAGAT TGATACCTCT - 47101 GCTGCACAAA AGGCAATCGA CCGAGCTGGA CCAGCGCATT CGTGACACCG TCTCCTTCGA - 47161 ACTTATTCGC AATGGAGTGT CATTCATCAA GGACNGCCTG ATCGCAAATG GTGCTATCCA - 47221 CGCAGCGGCA ATCGAAAACC CTCAGCCGGT GACCAATATC TACAACATCA GCCTTGGTAT - 47281 CCTGCGTGAT GAGCCAGCGC AGAACAAGGT AACCGTCAGT GCCGATAAGT TCAAAGTTAA - 47341 ACCTGGTGTT GATACCAACA TTGAAACGTT GATCGAAAAC GCGCTGAAAA ACGCTGCTGA - 47401 ATGTGCGGCG CTGGATGTCA CAAAGCAAAT GGCAGCAGAC AAGAAAGCGA TGGATGAACT - 47461 GGCTTCCTAT GTCCGCACGG CCATCATGAT GGAATGTTTC CCCGGTGGTG TTATCTGGCA - 47521 GCAGTGCCGT CGATAGTATG CAATTGATAA TTATTATCAT TTGCGGGTCC TTTCCGGCGA - 47581 TCCGCCTTGT TACGGGGCGG CGACCTCGCG GGTTTTCGCT ATTTATGAAA ATTTTCCGGT - 47641 TTAAGGCGTT TCCGTTCTTC TTCGTCATAA CTTAATGTTT TTATTTAAAA TACCCTCTGA - 47701 AAAGAAAGGA AACGACAGGT GCTGAAAGCG AGCTTTTTGG CCTCTGTCGT TTCCTTTCTC - 47761 TGTTTTTGTC CGTGGAATGA ACAATGGAAG TCAACAAAAA GCAGAGCTTA TCGATGATAA - 47821 GCGGTCAAAC ATGAGAATTC GCGGCCGCAT AATACGACTC ACTATAGGGA TCTTAATTAA - 47881 GGCGCCTGAT CAGGATCAGG TCGATGGCTT TGTTGTTCTC CGGGCAGCGC ACCGCCGTCG - 47941 GAAACTCGGC CTTGCCTTTG GCGAACGTGG TGTCGACGTA GGCGATGTTG ATGCCCTTGT - 48001 CTTCCAAGAA GCGCGCCACG TCGATGTTGT CCGGGTCTGC GCTGAAGTAC AGCGCCAGGT - 48061 TGTCGAGCCT CTGCGAGTGC AGGTAGACAG CCGCCGTCTG AACCCTTGTG TAGGCCCAGA - 48121 ACTGGACATC CGGGTTGTCG CGGATGACTC GACCCCAAGC GGCCACATAG GTGGGGCTGA - 48181 AGAAGTCTCC ATCCCAGTGG ATGCGGAACA GCTTCGGAGC CTTGCGACGG TCGCAATCCT - 48241 TGACGAACTC GGCGACCATC TCGGACAGCA GCGTCACGGT GTCTGTCAAG TCAGCGTCAC - 48241 TGACGAACTC GGCGACCATC TCGGACAGCA GCGTCACGGT GTCTGTCAAG TCAGCGTCAC - 48301 GCAACAGTTC CCAGTTGTGC AGCAGGACCG AGCTGACAGC CTTGCGAACT TTCTCCAGCT - 48361 TGCCGGCGTA GCACACCTTG GCACAGAAGG CCGTCGCGTC CGGGCAGGAG AAGCCTTGAC - 48421 CGGAGGGCAG GCCGATGCTG TTGGCGATAC CTACGGTGGC GTTGCCGCCC TTGGTGACGT - 48481 GGACGTAGTT GGTGACCTTG CGGTCGTTCG AACGCTTCAG CTTGGCCATA CCTAGCCTTC - 48541 CTTCGGTGGC TGTCAAGTTG TTGGATACAA AGCGCCCCGA GAGGGAGTCG AACCCTCACA - 48601 CCGCGAACCG TCGCGGGGCC ACCGTGCCTA GTCGATAGAG GTCACTCGAC TCTCGTGGAC - 48661 GTAGACCACG GTGTTGCCTA CGTTCACCGC GTAGTACAGG CCATCGGCAC CTCGTAGCTT - 48721 GTGCCGAACC GTGCCCGACG TGGCCGTCAT GTCTTCGCCC CAGTCGGCGT TAGGTGCCCA - 48781 GGTGACTCGC ATGGTGATCC CTTCAGTAGT CGGTGGCTGT CAAGTCAGCG GATACGGACG - 48841 TACCCGTTGC CTCGAGCGAC GTAGATCTTG CCGTCGATGT AAACGCGCTG CTGCTGGTTC - 48901 ATAATCCTAT TCCTTTCGGT GGCTGTCAAG TCTCAGGCCC AGCGACGAGT CGTCGGCCGG - 48961 GGGCGGCGCA CCTTGGGCGC GTTGGCTCGC GGTGCCTTAC GGATGGCGGT GCCTACCGTG - 49021 ATCTCTTCCA ACTGGCGTTC AGCCAGGCCG ACAGGCCGGG CGTCACCGGG CAGTTCGATC - 49081 TTGTAATCGA AGTCAGTCCA CCCCTTCAGA CCCTTCTCCA GCTCGCGATC CAACAGACGC - 49141 GGAGCCGACA GCTCAGGCGC AACAAACGGT GTCTTGACGC TCTCGCGGGC AGTAACCCGA - 49201 ACCTCACGGT GCTCAGCGAA GACTGGCATA GTTCACCCCT TTGGTGGATG TCAAGCCTGA - 49261 GCACCAAAGC TCAGGCGTAG TGGGTAGTCG GGAATCGAAC CCGATAGCTT CATAGCCACG - 49321 TTCTACGGCT CAGCCATAGC TCAGCGATCA TTCCATCGCG CCAAGAGCTA CCCTCCCGAA - 49381 TGCCGAACCA AAGCTCAGCA TTCGTAAGTG TGTATTCTCC CCGTGGCTCA GACAGTATCT - 49441 ATCAGAACCT AACCACAGGT CTACATTTAG TTATCCGCAG TGCTCGCACT TTAACGGCAT - 49501 CGAGCTTCCG CCGACCCTCA GTCCTCTGGC AGCGAACTAA AGGTTTGAGT CGGGCTGCGG - 49561 CCCTTCTCGG TCTTGCGTGA TTCTCACTCT ACCGGATGTT TCGGTGGCTG TCAAGCGGGC - 49621 CGTTTTGGTG TTGCAACGAT GCCCTCGTTT AGCGCCGCTG GCGTAATGCG CTACCCGCCT - 49681 GATCTCACCG GTCCAAGTTG GTGATGCTTG CAGCTTACCC GATAACCGGG TGGCTGTCAA - 49741 ACCGGAGAAT CTTGCCGCCG GATTTTCACC GGCACCGGCA CGATCCTCTC GGATCCGCCT - 49801 ACCGCCTTGC TGCTGCGGTG ACACAAGAAT GCACTACTGG CCGGGTGGCT GTCAAGCCCT - 49861 AATCGCAAAT TGGTGCCCTA GCTGCAGATA TGGCGCGTTC TCGGTGGCTG TAAAGGGCAC - 49921 TACGTGCCGC TATCCGCTGG TCACGCTGGA CAGTCCCGGC AGCCCGTGCC GCGCATAGGC - 49981 TGCTCACTAC GTGCCCGGTA TCGGCGTTGT CGTGCCGCTG TCGTGGTCGT CGCCCCGTCG - 50041 CTGTCGCTGG TCTCGGTGGC ATCGCTTGAC AGTCGCCCCG CTATCCCCCG TTGCCGCTGG - 50101 TCAGACGCTA ATCCGCTTAT TTCGCATAGG CTGCTCACTA TCGCATCGGT ATGCGTATGC - 50161 GCTGGTCACA TATGCGTGTG GTGGTGGTGT GGTGTGCGTG TGTTTGCGCT GGTCAGCCGT - 50221 GTGCGTACCG TATCCGCACA CTGTGCTTGT GCGTTTGCTG TGTGTCGAGG CCGGCTCTCG - 50281 CATCGTCGCA TGTCAGCGCG GGTATGGGCG TGTATCGCAC GCTTTGCTAG CCGCGTGCCG - 50341 C__________________________________________________________________________
EXAMPLE 3
Construction of L5 Polymerase Complementing M. smeqmatis Strains mc.sup.2 889 and mc.sup.2 890
Transposon delivery shuttle phasmids should not be able to replicate in the recipient strain to which the transposon is to be delivered. However, in order to maintain and propagate the shuttle phasmid, a conditional host in which this vector will be able to replicate should be available. In the case of phAE41 pol or phAE42 pol, this host should be one from which a high titer phage lysate can be obtained. Therefore, a trans-complementing derivative of M. smegmatis mc.sup.2 155 carrying the L5 polymerase gene integrated in its chromosome was constructed. phAE41 was cleaved with PvuII and SacII and 7620 bp fragment containing the pol gene, was agarose purified and subsequently cleaved with AsuII. Cleavage with the AsuII produced a 2211 bp internal DNA fragment containing the intact pol-gene. As a cloning vector, an integration proficient expression vector pMV361 was used (see Stover et al., Nature, Vol. 351, pp. 456-460 (1991)). The cloning vector was digested with ClaI, dephosphorylated and subsequently transformed in E. coli DH5.alpha. from Bethesda Research Laboratories. Kanamycin resistant colonies were screened for the presence and orientation of pol gene downstream from the hsp 60 promoter. An E. coli clone was selected. Plasmid DNA was isolated and electroporated into M. smegmatis mc.sup.2 155. Two types of kanamycin resistant colonies were isolated: small and large. The small colonies were designated mc.sup.2 889, and the large colonies were designated mc.sup.2 890.
EXAMPLE 4
Construction of int attP xis (phAE42) and pol (phAE45) Deletion Mutants of L5 Shuttle Phasmid phAE41
Since the entire sequence of the L5 genome is known the entire sequence of the shuttle phasmid phAE41 was deduced after determining the exact site of insertion. As discussed above, restriction analysis allowed for the determination of the site of insertion.
L5 shuttle phasmids have several advantages over L5 phages. Specifically, they are stable constructs for which the sequences can easily be deduced. In addition, they can be manipulated as cosmids in E. coli. These properties allow for easy manipulation of the L5 genome. Manipulation of the L5 genome of phAE41 shuttle phasmid was performed in order to produce shuttle phasmids phAE42 and phAE45, as described below.
In order to generate transposon delivery vectors, it is necessary that the delivery phage not be able to replicate in the recipient cell. Therefore, a derivative of phAE41 which was devoid of the integration functions of L5 was constructed. This construct was achieved by recombining a previously characterized mutant of L5, L5cd31 (see Donelly-Wu, et al., Molec. Microbiol., Vol. 7, pp. 407-417 (1993)), that contained a deletion of the region containing integration functions. In order to achieve this, the L5cd3l mutant and phAE41 were both cleaved with XbaI, an enzyme that cleaves each molecule twice. The XbaI-Fragment containing the deletion of L5cd31 was isolated and ligated to the purified arm containing E. coli cosmid of phAE41. These two fragments were ligated together and packaged into bacteriophage lambda heads. The resulting transducing particles were transduced into E. coli, and clones conferring ampicillin-resistance were identified. Two different orientations of the L5cd31 fragment were found in analyzed E. coli recombinants. The fragment in the correct orientation was capable of producing plaques following transfection of M. smegmatis mc.sup.2 155 cells. Restriction analysis confirmed that phAE41 had acquired the deletion of L5cd31, thus generating shuttle phasmid phAE42, to which the integration genes had been deleted. Shuttle phasmid phAE42 was deposited with the American Type Culture Collection, Rockville, Md., on May 20, 1994 and catalogued as ATCC No. 69625. This demonstrates that novel L5 shuttle phasmid derivatives can easily be constructed by common cloning methodologies and cosmid technologies known to those skilled in the art, which methods employ packaging in vitro techniques.
It was then determined whether specific genes are essential for the propagation of the L5 mycobacteriophage. The first gene studied was the polymerase (pol) gene of mycobacteriophage L5. A deletion of this gene was constructed in order to determine whether it is essential for replication of the L5 phage in M. smegmatis. To achieve this, the 7.6 kb PvuII-SacII fragment containing the pol gene of L5 was cloned into a pKS Blueskript derivative vector. An internal deletion of the pol gene was then generated with by cleaving this molecule with CleI. This removed 290 bp of the pol gene. The 7.3 kb PvuII-SacII L5 fragment containing the pol deletion was then mixed with the other purified PvuII-SacII fragments of phAE41, ligated, in vitro packaged, and in transduced in E. coli selecting for ampicillin-resistant colonies. Plasmids were isolated and characterized by restriction analysis and one out of 20 was found to have the full complement of the phAE41 parent, except that the 7.6 PvuII-SacII fragment had been replaced with the 7.3 kb deleted PvuII-SacII fragment. One such mutant was denoted phAE45, which was deposited with the American Type Culture Collection, Rockville, Md., on May 20, 1994, and catalogued as ATCC No. 69628. This demonstrates that specific deletion can be readily incorporated into specific genes of L5 and that the genome of the resulting phasmid can be readily amplified for subsequent analyses in M. smegmatis and other mycobacteria. In addition, transfection of 1 .mu.g of phAE45 DNA isolated from E. coli yielded no plaques following transfection of mc.sup.2 155 cells. In contrast, transfection of mc.sup.2 155 cells with 1 .mu.g phAE41 DNA yielded 2800 plagues. This inability of phAE45 to form plagues on mc.sup.2 155 establishes that the L5 pol gene is essential for L5 propagation.
EXAMPLE 5
Construction of L5 Luciferase Shuttle Phasmids phAE43 and phAE44
PacI, NotI and EcoRI sites flanking the unique BamHl restriction site of pYUB328 were incorporated into L5 shuttle phasmids. Since L5 does not contain PacI, NotI or EcoRI sites, L5 shuttle phasmids can be cleaved with any of these enzymes in order to release virtually the entire pYUB328 cosmid. This facilitates the incorporation of any new cosmid into the L5 backbone. In order to demonstrate this, phAE41 and phAE42 were cleaved with NotI, and the L5 sequences were isolated away from the cosmid portion of these shuttle phasmids. A ligation was set up between the isolated NotI fragment of phAE41 containing the L5 sequences and pYUB216 (see FIG. 5) by the method described by Jacobs, et al., Science, Vol. 260, pp. 819-822 (1993). The resulting ligation was in vitro packaged into E. coli and ampicillin-resistant colonies were selected for. The resulting cosmid molecules were screened. It was found that pYUB216 had been introduced into both orientations into the NotI backbone of phAE41. Transfection of the set of 4 different phAE41 and phAE42 derivative plasmids revealed that only those cosmids in which the Hsp60 promoter was in the same orientation as the leftward promoter of L5 yielded plaques. A single plaque derived from phAE41, which was designated phAE43 (see FIG. 6), was deposited in with the American Type Culture Collection, Rockville, Md., on May 20, 1994 and catalogued as ATCC No. 69626. A single plaque derived from phAE42, which was designated phAE44, was deposited with the American Type Culture Collection, Rockville, Md., on May 20, 1994 and catalogued as ATCC No. 69627. Both phAE43 and phAE44 were then characterized.
The L5 luciferase shuttle phasmids, phAE43 and phAE44, were compared with the TM4 derived luciferase shuttle phasmid phAE40, which was deposited with the American Type Culture Collection, Rockville, Md., on Apr. 29, 1993, and catalogued as ATCC No. 75457 for their abilities to generate light in M. smegmatis. FIG. 7 demonstrates that both phAE43 and phAE44 luciferase shuttle phasmids yielded the production of photons in M. smegmatis in 1-3 hours post infection with yields surpassing the phAE40 yield 48 hours post infection. As a control, TM4-derived luciferase shuttle phasmid phAE40 yielded a very rapid burst of light, with maximal yields two to three hours post infection. This demonstrates that L5 shuttle phasmids can deliver luciferase genes to mycobacteria. In addition, this shows that L5 shuttle phasmids allow for the altering of every gene to determine the effect of each gene on luciferase production, and thereby provide tools for the further development of luciferase reporter phages.
EXAMPLE 6
Generation of Insertion Mutations with IS1096-derived Transposons
As discussed hereinabove, it is desirable to obtain plasmids containing transposons for use in obtaining mycobacterial mutants. In order to perform this, several plasmids were utilized. Plasmids used in transposition experiments and their features are indicated in Table 1, below.
TABLE 1__________________________________________________________________________Bacterial strains, plasmids and transposable elementsStrain, plasmid, or transposable element Relevant characteristics Source or reference__________________________________________________________________________E. coli DH5.alpha. F .phi.80dl.alpha.cZ.DELTA.M15 endA1 recA1 hsdR17 glnV thi-1 Bethesda Research HU - gyrA96 relA1 .DELTA.(l.alpha.cZYA-.alpha.rgF)U169 Laboratories M. bovis BCG Pasteur strain Institut Pasteur mc.sup.2 789 M. bovis BCG[chr::Tn5367]met This study mc.sup.2 797 M. bovis BCG[chr::Tn5367]leu-1 This study mc.sup.2 798 M. bovis BCG[chr::Tn5366]leu-2 This study mc.sup.2 826 M. bovis BCG#12[chr::Tn5367] This study mc.sup.2 827 M. bovis BCG#13[chr::Tn5367] This study mc.sup.2 828 M. bovis BCG#14[chr::Tn5367] This study mc.sup.2 829 M. bovis BCG#21[chr::Tn5368] This study mc.sup.2 830 M. bovis BCG#22tchr::Tn5368] This study mc.sup.2 831 M. bovis BCG#23[chr::Tn5368] This study mc.sup.2 849 M. bovis BCG#11[chr::Tn5367] This study mc.sup.2 850 M. bovis BCG#15[chr::Tn5367] This study mc.sup.2 851 M. bovis BCG#16[chr::Tn5367] This study mc.sup.2 852 M. hovis BCG#17[chr::Tn5368] This study mc.sup.2 853 M. bovis BCG#18[chr::Tn5368] This study mc.sup.2 854 M. bovis BCG#19[chr::Tn5368] This study mc.sup.2 855 M. bovis BCG#20[chr::Tn5368] This study mc.sup.2 856 M. bovis BCG#24[chr::Tn5368] This study mc.sup.2 857 M. bovis BCG#25[chr::Tn5367] This study mc.sup.2 858 M. bovis BCG#26[chr::Tn5367] This study mc.sup.2 859 M. bovis BCG#27[chr::Tn5367] This study mc.sup.2 860 M. bovis BCG#28[chr::Tn5368] This study mc.sup.2 861 M. bovis BCG#29[chr::Tn5368] This study Bluescript II KS+/- pUC derivative, Amp.sup.r Stratagene pMV261 contains oriE, oriM and .alpha.ph genes (38) PYUB8 pBR322 derivative containing oriE, .alpha.ph and tet genes pYUB53 pYUB8 derivative containing oriE, oriM, .alpha.ph and tet genes (22) pYUB285 .DELTA.oriM, contains oriE and tet genes and Tn5367 This study pYUB297 .alpha.oriM, contains oriE and tet genes and Tn5368 This study pYUB305 .alpha.oriM, contains oriE and tet genes and Tn5369 This study PYUB312 .alpha.oriM, contains oriE, .alpha.ph and tet genes This study IS1096 M. Smegmatis insertion sequence (7) Tn5366 IS1096 derivative containing .alpha.ph gene This study Tn5367 IS1096 derivative containing .alpha.ph gene This study Tn5368 IS1096 derivative containing .alpha.ph gene This study Tn5369 IS1096 derivative containing .alpha.ph gene This study__________________________________________________________________________
All plasmids have a ColE1 origin and an aminoglycoside 3'-phosphotransferase (aph) gene from Tn903 encoding kanamycin/neomycin resistance. This aph gene was PCR amplified from pKD348 (Derbyshire) to include a trp transcriptional terminator for use in transposon constructs. pYUB8 additionally has a tet gene; pYUB53 was derived from pYUB8 with the addition of the entire mycobacterial origin of replication from pAL5000; and pMV261 has a fully functional oriM, consisting of ORFs 1 and 2 from pAL5000. The remaining plasmids containing IS1096 were constructed as described herein. The DraIIi deletion internal to the oriM was performed by digestion followed by T4 DNA polymerase treatment (Pharmacia). E. coli was transformed with plasmids by electroporation (Bio-Rad); or the CaCl.sub.2 procedure using pretreated cells (Bethesda Research Laboratories). Plasmids were prepared from E. coli by both Birmboim/CsCl.sub.2 and column (Quiagen) methods. M. bovis BCG cells were transformed by electroporation after washing in 10% glycerol, and then 4 mls complete media M-ADC-TW containing 0.5% casamino acids and 20 .mu.g/ml tryptophan were added, followed by incubation overnight at 30.degree. C. and plating on Middlebrook 7H10 with glycerol, ADC, cyclohexamide, amino acid supplements and kanamycin at 20 .mu.g/ml. Colonies were counted after 3 weeks incubation at 37.degree. C.
In order to perform Southern blotting and hybridization, single M. bovis BCG colonies were grown in 10 mls MADCTW containing kanamycin and expanded 1:50 for preparation of DNA. Whole DNA was prepared from 50 ml cultures, by a ten fold scale up of the CTAB method (see van Soolingen et al., J. Clin. Microbiol., Vol. 29, pp. 2578-2586 (1991)). DNA concentration was estimated by agarose gel electrophoresis and approximately 2 .mu.g was digested with restriction enzyme and run on a 0.7% or 1% agarose gel at 40 V overnight. The DNA was transferred to nylon membrane (ICN). Hybridization was performed using plasmid pYUB285 as a probe labelled with [.alpha.-.sup.32 P]dCTP as described by Cirillo et al., J. Bacteriol., Vol. 173, pp. 7772-7780 (1991). The sizes of fragments hybridizing on KpnI and BamHI Southern blots were estimated using the mobilities of standard DNA markers run on each gel.
In order to isolate integrated transposons and perform sequence analyses, KpnI-digested fragments containing the integrated transposon were cut from an agarose gel and cloned into Bluescript II KS+ (Promega) using kanamycin selection. Outward primers based on the sequence of IS1096 were used with the Sequenase version 2.0 and the Longranger (United States Biochemical) acrylamide gel reagents. Sequences of the insertion sites were obtained for both DNA strands.
Auxotrophs were then isolated. Colonies obtained on pYUB284 and pYUB297 transformation were picked using wooden sticks into 96-well plates containing complete medium. They were grown up and washed twice in minimal (M-ADC-TW) medium before being replicated onto agar plates with and without amino acid supplement using a 96-prong template (Dankar). Candidates were further streaked from the original 96-well plate to check their auxotrophy, and auxonographic analysis was performed on washed cultures as described by Kaplana et al., Methods Enzymol., Vol. 204, pp. 139-180 (1991).
IS1096-derived transposons were constructed. The kanamycin resistance gene (aph) from Tn903 was PCR-amplified and cloned into the unique SalI, MluI or NcoI sites in the insertion sequence IS1096. This created a set of three transposons, having the aph gene in each of the open reading frames of IS1096, as well as between them. The elements are shown in FIG. 8. Tn5368 has the aph gene inserted into ORFR, Tn5369 has the insertion in ORFA, and in Tn5367, the aph gene does not disturb either ORF. Tn5366 is identical to Tn5367 but has the aph gene in the reverse orientation. TnpA and TnpR have been denoted ORFA and ORFR, respectively.
In order to construct transposon delivery plasmids, IS1096, with its adjacent lacZ sequences, (100-200 base pairs on each side), was cloned in both orientations into the multicloning site of pGEM7Zf+ (Promega) to create plasmids pYUB234 and pYUB235, thereby enabling excision of the element with EcoRI and HindIII. A third vector, pYUB272, was constructed, with EcoRI and HindIII sites, as well as origins of replication for E. coli and mycobacteria and a tetracycline resistance,. tet gene. This plasmid was derived from pMV261 by replacement of the NotI-PstI fragment with a tetR gene obtained by PCR from pYUB53. The transposons Tn5368 and Tn5369 which were created on pYUB234 were inserted into pYUB272 by ligation after digestion with EcoRI and HindIII. In order to obtain a delivery plasmid which is unable to replicate in mycobacteria, the mycobacterial origin of replication (oriM) was inactivated in each of the constructs by an internal deletion using DraIII, which removed 556 base pairs of DNA and a significant part of an open reading frame in the origin of replication.
Tn5367, having an aph gene in the MluI site, was created after ligation of the IS element to pYUB272. Insertion of the aph gene into the MluI site of the IS element necessitated a partial digestion since there is also an MluI site in pYUB272. The construct obtained from insertion into this second site provided a plasmid having the aph gene outside the transposon, which could then be used as a control to monitor any illegitimate integration of the plasmids. DraIII deletions within the oriM were also performed on these two plasmids. The four plasmid delivery constructs, pYUB285, pYUB297, pYUB305 and pYUB312, are shown in FIG. 9.
M. bovis-BCG with transposon delivery constructs were transformed. The numbers of kanamycin-resistant colonies resulting from five transformation experiments are shown in Table 2, below. In addition to the transposon delivery plasmids, three additional plasmids were used as controls. The efficiency of transformation was determined with an oriM-containing vector. Either pMV261, or pYUB53 and any illegitimate integration was monitored using either one of two plasmids: pYUB312 with oriM deletion but the aph gene outside the insertion element; or pYUB8 which has no insertion element or mycobacterial origin, but has an aph gene. A control of cells alone having no plasmid was also electroporated and plated in an identical fashion to the samples to estimate the level of spontaneous resistance to kanamycin ("background").
TABLE 2__________________________________________________________________________M. bovis BCG transformation PlasmidExperiment.sup.a pYUB285 pYUB297 pYUB305 pYUB312 pYUB8 none oriM.sup.+ /.mu.g.sup.c__________________________________________________________________________1 255 -- -- -- 14 -- 4.5 .times. 10.sup.4 2 155 104 -- -- 16 31 1.5 .times. 10.sup.4 3 .sup. 426.sup.b 225 -- 59 -- 60 5 .times. 10.sup.4 4 67 98 18 3 -- 6 4.4 .times. 10.sup.4 5 325 -- 60 41 -- 36 1.3 .times. 10.sup.5__________________________________________________________________________ .sup.1 Within each experiment, the same amount of plasmid was transformed Experiment 1: 02 .mu.g; Experiment 2: 5 .mu.g and Experiment 3-5: 400 ng, except; .sup.b 1 .mu.g plasmid was used. .sup.c For the oriM.sup.+ control plasmid, 0.2 .mu.g-0.4 .mu.g was used, and numbers are represented as transformants per .mu.g. Two different plasmids were used; Experiments 1 and 2: pYUB53 and Experiments 3-5: pMV261. .sup.d Recovery conditions after electroporation were 37.degree. C. instead of 30.degree. C. -- Dashes represent data not obtained.
The results show that in each experiment there is a transformation frequency of 10.sup.4 -10.sup.5 /.mu.g with the oriM-containing plasmid, and a number of kanamycin-resistant colonies arising from transformation with pYUB285 or pYUB297. These numbers are well above background from any of the controls and shows transposition. Furthermore, while pYUB285 contains Tn5367 having both ORFs of the IS element intact, pYUB297 carries Tn5368 in which ORFR is disrupted, implying that this ORF is not required for transposition. In addition, the numbers of colonies obtained for pYUB305, the construct carrying Tn5369, in which ORFA is disrupted, are sharply reduced to close to background levels, suggesting that this ORF is required for transposition and is likely to be the transposase. There is no evidence of illegitimate integration of plasmids since transformation with pYUB312 or PYUB8 yields numbers at background levels.
In order to perform analysis of kanamycin-resistant colonies using Southern blotting, twenty colonies arising from transformation of pYUB285 and pYUB297 were picked at random, grown up in the presence of kanamycin and examined by Southern blotting using three different enzymes and plasmid pYUB285 as probe. The Southern blot results of six of such clones, strains mc.sup.2 826-mc.sup.2 831, are shown in FIG. 10. Using KpnI, one fragment hybridizes in each clone (see FIG. 10A), since there is no KpnI site in the transposon. To verify that insertion was random, BamHI was also used. This enzyme cuts once within the transposon, and when probed with plasmid, two bands are seen, corresponding to fragments obtained from the transposon's insertion into the chromosome (see FIG. 10B). These fragments appear to be of random size, and indicate that mc.sup.2 830 and mc.sup.2 831, which yield similar-sized KpnI fragments, are not identical clones.
Digestion with PvuI and hybridization with plasmid was carried out in order to determine whether any plasmid sequences were present which would suggest either the presence of cointegrate intermediates or an illegitimate recombination event. The results are shown in FIG. 10C. Digestion of the delivery plasmids with PvuI yields several fragments, including one internal to the transposon (see FIG. 9). If transposition had occurred by simple insertion rather than replication and cointegrate formation, the expected bands from the transposon-containing clones would include this internal fragment and two additional bands resulting from the insertion of the transposon into the chromosome. Three bands were seen with Tn5367 insertions mc.sup.2 826, mc.sup.2 827, mc.sup.2 828, each with expected internal band as well as two unique bands of differing size. Results for the clones transformed with Tn5368 show that one of the clones (mc.sup.2 829) gives three bands, but the other two (mc.sup.2 820 and mc.sup.2 831) display only one additional band along with the expected internal fragment, suggesting that deletions of the transposon are occurring. The absence of extra bands, however excludes the possibility of plasmid integration.
Of the 20 colonies analyzed in total, one clone did not hybridize on Southern blot, suggesting that its kanamycin resistance was due to a spontaneous mutation. The results of Southern blots of the remaining 19 clones with KpnI and BamHI were analyzed and are represented graphically in FIG. 11, to illustrate the random nature of insertion of Tn5367 and Tn5368 into different restriction fragments in each clone. The KpnI fragment sizes were plotted in descending order and the BamHI fragments corresponding to each lone are superimposed. This representation of the data was chosen so that results for each enzyme could be combined to show that the insertion site of the transposon differs for each clone. Even clones showing similar sized KpnI fragments differ in the size of the BamHI fragment into which the transposon was inserted. No plasmid sequences were detected after Southern blotting using PvuI.
Insertion junctions were then sequenced. IS1096 was previously found to create 8-base pair direct repeats on insertion into its target site (see Cirillo et al., J. Bacteriol., Vol. 173, pp. 7772-7780 (1991)). Therefore, sequencing was performed on the six clones (mc.sup.2 826-mc.sup.2 831) described above in order to confirm that the transposons retained this property, and in order to investigate any target-site preference. The sequences of the duplicated target sites are given in FIG. 12. There is a weak consensus at the insertion junctions of XXXTA/TXC/GX, where T always stands at position 4, and there is a preference in the target site for an AT-rich center and GC-rich ends. No similarities were seen between clones comparing 50 base pairs of flanking DNA.
Auxotrophic mutants were isolated and characterized. 923 kanamycin-resistant colonies resulting from the first and second experiments (see Table 2) were arrayed in 96-well plates, grown up, washed and tested for auxotrophy by patching onto plates lacking amino acid supplement. Candidates were tested in auxonography and three auxotrophs were found, one for methionine (Mc.sup.2 789) and two for leucine (mc.sup.2 797 and mc.sup.2 798). The leucine mutants appeared to be distinct, as mc.sup.2 798 grows more slowly than mc.sup.2 797. The growth of all three auxotrophs could be supported in liquid or solid minimal media by the addition of the relevant amino acid.
A transposon capable of random insertional mutagenesis in the M. tuberculosis complex allows for the isolation of auxotrophic mutants of M. bovis BCG and M. tuberculosis, which can be used for vaccine development and in new approaches to the study of virulence, such as in vivo expression technology (IVET). It also facilitates the analysis of mechanisms of invasion and survival in the host, and can lead to the identification of new drug targets in M. tuberculosis. The ability to mutate genes and locate them easily, is essential in the M. tuberculosis complex where gene replacement by homologous recombination prior hereto, has been is difficult.
The inventors have shown that transposons derived from IS1096 do transpose in BCG. The products of transposition appear to be the insertion of one copy of the transposon, with no cointegrates or plasmid sequences. The inference that ORFA is a transposase, and should be referred to as TnpA, is also supported by the recent isolation of insertion element IS1001 from Bordetella pertussis and ISAE1 from Alcaligenes eutrophus, which both have an open reading frame with homology to ORFA of IS1096 (see Van der Zee et al., J. Bacteriol., Vol. 175, pp. 141-147 (1993) and Kung et al., J. Bacteriol., Vol. 174, pp. 8023-8029 (1992)). Tn610 from M. fortuitum and transposon derivatives of IS900 from M. paratuberculosis, transpose by a replicative mechanism which can involve cointegrate formation (see Martin et al., Nature, Vol. 345,k pp. 739-743 (1990) and England et al., Mol. Microbiol., Vol. 5, pp. 2047-2052 (1991)). It is not uncommon for insertion sequences to exhibit more than one open reading frame. These can be involved in the transposition mechanism, or in the regulation of transposition. If ORFR is indeed a resolvase, it is not required for transposition or resolution in M. bovis BCG. However, two of the clones analyzed by Southern blot using PvuI showed evidence of deletions, and both of these clones were derived from transformation with Tn5368, having an insertion in ORFR.
In nineteen out of twenty clones isolated at random, the transposons were found in different sites in the chromosome, as evidenced by restriction fragment size on Southern blotting. In each of the six insertions sequenced, a target duplication of eight base pairs was seen on either side of the element. The DNA sequence of the insertion site differed in each clone analyzed, with only a weak consensus, indicating that there is little constraint on insertion site. Two of the clones have the sequence ATAA at the center. The related IS1001 and ISAE1 both recognize AT-rich targets. IS1001 recognizes runs of As or Ts, and ISAE1 has a preference for A or T at the ends. Both bacteria are also high %G+C organisms. The random nature of the transposition and the ability of the transposons to insert into coding regions is also demonstrated by the isolation of methionine and leucine auxotrophs in BCG.
EXAMPLE 7
Characterization of Methionine and Leucine Auxotrophs of BCG Generated by IS1096-derived Transposons
IS1096-derived transposons are able to transpose in BCG. In order to show this, a library of approximately 1000 random insertions of IS1096-derived transposons in BCG was screened for the ability to grow on minimal media after originally selecting on media containing casamino acids. Three auxotrophic mutants of BCG were identified. Auxonography analysis revealed that two of the auxotrophs were leucine-requiring mutants, and one was a methionine-requiring mutant. The reversion frequencies were determined to be between 10.sup.-7 to 10.sup.-8, as shown in Table 3, below. When the BCG auxotrophs were then grown in mice, it was determined that the auxotrophs possessed very properties. The methionine auxotroph resembled BCG when inoculated in mice. In contrast, both of the leucine auxotrophs were cleared rapidly from both the spleens and lungs of the mice (see FIG. 13). This confirms that the IS1096-derived transposons caused different mutations in BCG.
TABLE 3______________________________________M. bovis-BCG Strains Strain Genotype Phenotype Reversion Frequency______________________________________BCG-Pasteur wild-type prototroph NA mc.sup.2 797 leuD1::Tn5367 leucine auxotroph 1.6 .times. 10.sup.-7 mc.sup.2 798 leuD2::Tn5366 leucine auxotroph 2.0 .times. 10.sup.-7 mc.sup.2 789 met-2::Tn5367 met auxotroph 1.0 .times. 10.sup.-7 mc.sup.2 796 zzz::Tn5366 prototroph NA______________________________________
EXAMPLE 8
Construction of L5 Transposon Delivery Vectors phAE46 and phAE47
By utilizing the cosmid replacement technology in L5 replacement shuttle phasmid phAE41, the pYUB328 cosmid was replaced by the cosmid pYUB435. The pYUB435 cosmid was cloned into the EcoR1 site of both phAE41 and phAE42 in order to generate L5 shuttle phasmids phAE46 (see FIG. 14) and phAE47 (see FIG. 15), respectively. The resulting transposon delivery vectors phAE46 and phAE47 were deposited with the American Type Culture Collection, Rockville, Md., on May 20, 1994, and catalogued as ATCC Nos. 69629 and 69630, respectively. Both of these vectors contain the IS1096-derived transposon Tn5367 which was shown to be able to transpose and make mutations in BCG (see Examples 6 and 7).
Although the invention herein has been described with reference to particular embodiments, it is to be understood that these embodiments are merely illustrative of various aspects of the invention. Thus, it is to be understood that numerous modifications may be made in the illustrative embodiments and other arrangements may be devised without departing from the spirit and scope of the invention.
__________________________________________________________________________# SEQUENCE LISTING - - - - (1) GENERAL INFORMATION: - - (iii) NUMBER OF SEQUENCES: 1 - - - - (2) INFORMATION FOR SEQ ID NO:1: - - (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 50341 (B) TYPE: nucleic a - #cid (C) STRANDEDNESS: sing - #le (D) TOPOLOGY: linear - - (ii) MOLECULE TYPE: (A) DESCRIPTION: L5 shu - #ttle phasmid sequence - - (iii) HYPOTHETICAL: No - - (iv) ANTI-SENSE: - - (v) FRAGMENT TYPE: - - (vi) ORIGINAL SOURCE: (A) ORGANISM: L5 my - #cobacteriophage (B) STRAIN: (C) INDIVIDUAL ISOLATE: (D) DEVELOPMENTAL STAGE: (E) HAPLOTYPE: (F) TISSUE TYPE: (G) CELL TYPE: (H) CELL LINE: (I) ORGANELLE: - - (vii) IMMEDIATE SOURCE: - - (viii) POSITION IN GENOME: (A) CHROMOSOME/SEGMENT: - - (ix) FEATURE: (A) NAME/KEY: (B) LOCATION: (C) IDENTIFICATION METHOD: (D) OTHER INFORMATION: - - (x) PUBLICATION INFORMATION: None (A) AUTHORS: (B) TITLE: (C) JOURNAL: (D) VOLUME: (F) PAGES: (G) DATE: (H) DOCUMENT NUMBER: (I) FILING DATE: (J) PUBLICATION DATE: (K) RELEVANT RESIDUES I - #N SEQ ID NO: - - (xi) SEQUENCE DESCRIPTION: SEQ ID NO: - # 1: - - GGCGCTCTCG CATCGCATCG AGTGTTTGCT GTGTCTCTCA TCGTCGCAGG TC -#AGAAGGGG 60 - - TAGGGGGGTT CCCCCTAGGG GTCGGTCCTT GACCGGTCGG TTAGGTCGGT TA -#TGCGGCCG 120 - - AGCCATCCTG TACGGGTTTC CAAGTCGATC AGAGGTAGGG GCCGGCACAG AA -#ACCACTCA 180 - - CATCAGGGCT GTGCGCCTCC AGGGCGCGTG AACTCCCACA CCCCGGTGTA GT -#TACATCCC 240 - - GGAATTGTCT CAGCGCCTCT CAGGGCGCTT CTCATAAACA GTGATCTACG CC -#ACTCCTGA 300 - - CGGGTGGCTG TCAAGGATAC TCACCTTCCC TACTAATGAG GGGCTAAGAG CC -#CCTCTCTA 360 - - TAGAGCGCCG CACAGGCGGC GCGATAAGAG CGCCACCAGG CGCTCATCTA AA -#GACCGGCC 420 - - TTGAAGGGCC GGTCATAGAG ATCTATTCGA TCCGGCAACC GCCGGATCTC AA -#GGCCGCGC 480 - - CAGTGCGCGG CCCTATAGAG GGGTGACTCA ACTGTGCATG GCACTCGCTC GA -#GTGCCCAC 540 - - TGGAGCACTC AACCGGGGAA GTTCGACGTT CTCAACCTGC GAATGACGTT TG -#AATCGTCA 600 - - TCCGCGTACG AAATCCCCGA TCTGCGGCCG ACCGACTTCG TGCCGGCCTA TC -#TCGCGGCC 660 - - TGGAATATGC CGCGTCACCG CGATTACGCC GCCAAGAACG GCGGCGCGCT GC -#ACTTCTTC 720 - - CTTGACGATT ACCGGTTTGA GACCGCGTGG TCGTCCCCCG AGCGCCTTCT CG -#ACCGCGTA 780 - - AAGCAGGTCG GCGCTGCACT CACGCCGGAT TTCAGCCTCT GGACGAACAT GC -#CGAAGGCG 840 - - GCGCAGCTAT GGAACGTCTA CCGCTCCCGC TGGTGTGGCG CGTATTGGCA GT -#CGGAAGGA 900 - - ATCGAGGTGA TTCCGACGGC GTGTTGGGCG ACTCCCGACA CGTTCGATTT CT -#GTTTCGAC 960 - - GGGATCCCGA TGGGATCGAC CGTCGCAATT TCTTCGATGG GCATTCGCTC TT -#CAAAAGTC 1020 - - GACCAGGAGC TTTTCCGGTA CGGACTACGC GAACTCATCG ATCGCACTCA AC -#CGCAACTG 1080 - - CTTTTGGCAT ATGGCCAGCT TCGGCATTGC GACGACATGG ATTTACCAGA GG -#TCCGCGAA 1140 - - TACCCGACCT ACTGGGACAG ACGACGAAAG TGGGTAACTG CCGATGGGAG GC -#CGGGGAAG 1200 - - TAAAGGCGGC CCCGGTCCCG GAACCGGAGC ACGCAACCGC AGAGGCGCTG GA -#GCCCCCGG 1260 - - ATCGGGCGGC GTAGGCGGCG TCGGAGGCGG GGGTGGAGCT GCAGGGAGCA GC -#GGAGGCGG 1320 - - CAAGGGAACG GCAGCGCCGG TACCGGAGGC GTCACCGGTG GCGGCGGAAG TG -#GAGCCGGC 1380 - - GGCGGTGGCA GCAGCCCCAA CACCCCGGTG CCCCCCACCG AGCTGGAGAA GA -#AGCGCGGC 1440 - - GAATACAACC AGATCGCCAT CGACGCCCAG AAACAGCACG CGCCCACCGA TG -#AGAAGCGC 1500 - - GAGGCCAAGC GCAAGCAACT GATGGATCGA GTCGGAGGAG ACTGGCAGGC TT -#TGGACCCG 1560 - - GATCACCACG ACGCCATCAA GGTGGCGATG GATGACGCCA TGCGGAAGAT CC -#TCTCCGAG 1620 - - GAGGAGATCG TCCACCGCAC CAAGCACTTC GGCGACCTAC TCGACTCCGG TC -#GACTCAAG 1680 - - TCGCTGTTCG AGGTCGGCTT CTCAGCCGGT GGCGACACCC CGACCGAACG CG -#CCCTCCTC 1740 - - GAGGACGCCT GGTTCGGCGC AGGCAAGGTT CCCCCGATCT ACTCGGCAAT CG -#AGTTCAAC 1800 - - GGCGCTCCGA CAGCCGGCCT CGGCATGTAC GGCGGCACCA AGCTCTACAT GA -#AGGACTCG 1860 - - GTCAAGGACC GCGTCACCGT GACCATCGGC GACTCGCTGA TGTCGAGCTG GG -#ACGTATTC 1920 - - CCCGGCCGTC CTGGCGACGG CGTGGGGCTG TGGGCCAGCC TGTCGAAGAT CG -#AGGGGCTG 1980 - - GTCGATCCGA GCAAGACCCG CGAAGAGAAC ATGCAGGCGG TGTACGACTC GT -#TCAAGAAG 2040 - - TACGGCACCC TGGACGGCTT CATCGAGGCG CAGATCCACG GCGGCGTCCT GG -#TCGAGGAC 2100 - - ATCAAGAAGG TCGTGTTCAC GCAGCCGCCG AGCCCGATCT TCACCGATAA AC -#TGGACGAA 2160 - - CTTGGAATCC CGTGGGAGGT GCAGTAATGG CGCAGATGCA GGCGACACAC AC -#AATCGAGG 2220 - - GGTTCCTGGC TGTCGAGGTG GCCCCTCGGG CGTTCGTCGC AGAGAACGGC CA -#CGTACTGA 2280 - - CCCGGCTGTC GGCCACGAAG TGGGGCGGTG GCGAGGGTCT CGAGATCCTC AA -#CTACGAGG 2340 - - GTCCAGGGAC CGTCGAGGTC TCCGACGAGA AGCTCGCCGA AGCCCAGCGG GC -#CAGCGAGG 2400 - - TCGAGGCTGA ACTTCGCCGC GAGGTCGGCA AGGAGTGAGC TGGGCCGGCT CA -#GGCCGGCG 2460 - - ACAGGAACTA CCAGAGGACT GGGAGCTGAA TTACCGGCTC CCGGTCCTTT CT -#GCTGCCAA 2520 - - CTGGCTTTGC CAGATCAACG GTCCCGGATG CGTAAGGGCC GCAACCGATG TC -#GACCACAT 2580 - - CAAGCGCGGG AACGACCACA GCCGGTCCAA TCTGCAGGCA GCCTGCCATG TC -#TGTCACGG 2640 - - CAAGAAATCA GCCGCCGAGG GCGTAGCCCG ACGGCGGGAA CTTAGAGCCC GG -#AGGAAGCG 2700 - - ACCACCCGAA CGCCATCCTG GGCGTCGATA AGCGGGCCAG GTGCCCGCTC CA -#CCCAGGAG 2760 - - GTGAACAGTG GGCACGCGAG GCCCAATCGG AAAACGAGAT GAAGAGCGGG TT -#CGTCGGAA 2820 - - CACCCCGGAC AGTCCAACCG ACACGATCCA GATGCCCGGT CTGGTGACGA TC -#CCCGAGAT 2880 - - GGGCGATCTA AGCCACGACG GCCGCACGCA CCAGCTCGTC AAGGACATGT AC -#GAGTCGAT 2940 - - CAAGCAGTCG GCAGCCGTGA AGTACTACGA GCCGACCGAC TGGCAGATGG CC -#CGACTCGC 3000 - - CCTCTACACA CTTAACCAGG AACTCATCGC AGCCGAGAAC AACGGCAAGC CC -#GTGGGCGC 3060 - - GATGAAGCTC ACTGCCATCA ACCAGATGCT CTCCGCGCTG CTGCTGACCG AA -#GGTGACCG 3120 - - ACGCCGCGTC CGACTCGAAG TCGAACGAGC ACCCGCTGAC CCGACAGGCG GG -#AAGGTCGT 3180 - - TGACGTGACC GACGTGCTCA AGCAGCGCCT CGCCAAGGCG AGCGGCGGGA GC -#TGATGGTC 3240 - - CCCCGAGGGG TTTCTAGAGC CGCTGCCGCT ACCAGCCGCT CCCCCTCGGG GT -#AGACATCG 3300 - - AAAGGAACCA CATGGCCGAC CTCGGCAACC CACTCGACCT CGAGATGCTC TG -#CCTGGTCA 3360 - - CAGGCCGGGA CTTCCGCTGG ACCATCGATT ACCCGTGGGG TCCGGGAGAG CT -#GTTCCTCG 3420 - - AACTCGAGAC CGGCGGCGAA CACAACGCGC TGCATCAGGT CTATGTCACC GG -#GGCGACCG 3480 - - GAGGCACGTA CACGCTGAAC GTCAACGGCA CCAACACCCC GGCCATCGAC TA -#CAACGACG 3540 - - TGTCGGAGAA TCCGCAGGGG CTGGCAGGCG ACATCCAAGA CGCTCTGGAC GC -#AGCCGTCG 3600 - - GAGCCGGAAA CGCTGTCGTG CATCCGGTCT CGCTGTTCCC TGCGTGGACA CT -#GAACTTCA 3660 - - ACCTCAACGC CAGCAAGCCG CTCACCGAGC AGTTGGTCAA CACGATCAAC AA -#GGCCGCGA 3720 - - ACGACTTCTT CGACACGTTC GACCAACTAC TTGGGGTCGA CGTGGAGATG AC -#GGTCACCG 3780 - - ACACCCTGAA CTTCAAGCTC AAGGTGACCT CGCGGCGCTC GTTCGATGAG GT -#CGGTGTCG 3840 - - TCACGTTCGC GGTCGACGTG ACCAGCCAGG CAGTCATCAA CTTCTTCAAC TC -#CGTCGCCG 3900 - - AACTCACCGG AGCGGTGAAC ACCGTCAACG TCGACTTCTA CTGGAACCGG AC -#GTATGACA 3960 - - TCGAGTTCAC CGGATCCCTT GGGCTGCAGC CGATTCCGGC TACTACAGCC GA -#CATCACCA 4020 - - ACCTGGCGGG TACCAGCAAG GCCGTCTCAG TCACGGTGGT CGAGCCAGGA AA -#GAAGAGGC 4080 - - TGACCATCTG GCCGTTCACG GTCAACGGTG AAACCGCAAC CATCAAGGTC GA -#GTCCGAAG 4140 - - AGGCCGACAA GATCCCCAAC CGCTGCCGCT GGCAGTTGGT TCACATGCCG AC -#CGGCGAGG 4200 - - CAGCCGGCGG CGATGCAAAG CAGCTCGGCC GCGTTTACCG ACAGCCGAGG TA -#ACACCGCA 4260 - - CCCATCAGAG ATGGTGGGCC AGACGGCCTT CGGGCCGTCC CCTGACGTGT AG -#CTCAATGG 4320 - - CAGAGCGCCC GACTGTTAAT CGGGTGGTTG AAGGTTCGAG TCCTTCCATG TC -#AGCGAGGG 4380 - - CTGAACCGGA CCCGTGTCCG GTGTAGGCAC TTTCCGCAGG CGGTTCCCCA GA -#GCGTGGGG 4440 - - AGCCCCTGCC CTGTACACGT AGCTCAATTG GTAGAGCAGC GGTCTCCAAA GC -#CGCCGGTT 4500 - - CCAGGTTCGA CTCCTGGCGT GTATGCACAC ACCCCTGACT CCTGCTAGCG GA -#GTGTTCGC 4560 - - CTTTCGGGCC TGGGGTCTTT TTCCCCGTTC GTCTAATCGG TAAGACACCC GG -#CTCTGGAC 4620 - - CGGGCAATTG AGGTTCGAGT CCTTGGCGGG GAGCCAACTT GACATCCACC CG -#AAAGGAAC 4680 - - AACATGACCT TCACAGTCAC CCGCGAGAGA GCGCAGTGGG TCCACGACAT GG -#CCCGCGCT 4740 - - CGCGACGGTC TCCCCTACGC GTACGGCGGG GCGTTCACCA ACAACCCGAG GG -#TGTCGACT 4800 - - GACTGCTCTG GCCTGGTGCT GCAGACCGGG GCTTGGTATG GAGGTCGCAC CG -#ACTGGGTC 4860 - - GGAAACCGTT ACGGCTCAAC CGAATCGTTC CGGCTCGACC ACAAGATCGT CT -#ACGACCTA 4920 - - GGGTTCAAGC GGATGCCCCG AGGCGGGCCA GCGGCCTTGC CGATCAAGCC GG -#TGATGCTC 4980 - - GTCGGGCTCC AGCACGGAGG CGGCGGGGTC TACTCGCACA CCGCTTGCAC GT -#TGATGACG 5040 - - ATGGACCACC CCGGTGGCCC GGTCAAGATG TCCGACCGAG GCGTCGACTG GG -#AGTCCCAC 5100 - - GGCAACCGCA ACGGCGTAGG CGTCGAACTT TACGAGGGCG CACGGGCATG GA -#ACGACCCT 5160 - - CTGTTCCATG ACTTTTGGTA CCTGGACGCA GTCCTCGAAG ACGAAGGAGA CG -#ATGACGAA 5220 - - TTGGCTGACC CAGTTCTAGG GAAGATGATC CGCGAGATCC ACGCGTGCCT GT -#TCAATCAG 5280 - - ACCGCGTCGA CCAGCGATCT GGCGACCCCT GGTGAAGGCG CTATCTGGCA GC -#TACACCAG 5340 - - AAGATCCACT CGATTGACGG CATGCTCCAC CCGATCCACG CTGAGCGGCG CG -#CTCGCGCA 5400 - - GGCGATCTCG GTGAGCTGCA CCGAATCGTG TTGGCCGCGA AGGGCTTGGG CG -#TGAAGCGC 5460 - - GACGAGGTGA CCAAGCGGGT CTACCAGAGC ATCCTCGCCG ACATCGAGCG GG -#ACAACCCC 5520 - - GAAGTACTTC AGCGATACAT CGCAGAAAGA GGTGGCCTAT GAGCCCCAAG AT -#CCGACAGA 5580 - - CCATCTACCT GCTCGGCACC GCCGCCCCGG CACTGCTGGG CATCGTCCTG AT -#CTGGGGCG 5640 - - GGCTCGACGC TGAGTCGGCG GCTGACCTCG GTGACATCAT TGCGGGCGTC GT -#GTCGATAC 5700 - - TAGTCTCCGG TGCGCCGGCC GTAGCGGCAG GCACCGTACG CAGCCAGCGC AA -#GGACGGCA 5760 - - CGTTGTCCAC CAGCCCGGTG GATCAGGTCA CCAAGGGCGT CGAGCAGGTG CT -#CGCGGCCA 5820 - - GGCAGAGTGC CGAGGCTGAA GTCGCGAAGG TCAAGCAGGC GCTGGAGACC GC -#CGTCAGCG 5880 - - GTTCTCTCCC CCAGCTCGGC CCGCTGGCCA CGCAGATCCT CAACGTGGCT GA -#CGACACCG 5940 - - TCTGGCGTCC ATGAGCAAGC CCTGGCTGTT CACCGTCCAC GGCACAGGCC AG -#CCCGACCC 6000 - - GCTCGGGCCT GGTCTGCCTG CCGATACCGC ACGGGACGTA CTTGACATCT AC -#CGGTGGCA 6060 - - GCCCATCGGC AACTACCCGG CAGCGGCGTT CCCGATGTGG CCGTCGGTCG AA -#AAGGGTGT 6120 - - CGCTGAGCTG ATCCTGCAGA TCGAGCTGAA GCTGGACGCA GATCCGTACG CG -#GACTTCGC 6180 - - GCTGGCCGGC TACTCGCAGG GAGCCATCGT GGTGGGCCAG GTGCTCAAGC AC -#CACATCAT 6240 - - CAACCCGAGA GGTCGACTGC ACCGGTTCCT GCACCGGCTC AGGAAGGTCA TC -#TTCTGGGG 6300 - - TAATCCGATG CGGCAGAAGG GCTTTGCCCA CACCGACGAG TGGATTCACC AG -#GTCGCTGC 6360 - - CTCGGACACG ATGGGCATCC TCGAGGACCG ACTGGAGAAC CTCGAGCAGT AC -#GGCTTTGA 6420 - - GGTCCGCGAC TACGCGCACG ACGGCGACAT GTACGCCTCC ATCAAGGAGG AC -#GACATGCA 6480 - - CGAGTACGAG GTGGCCATTG GCCGAATCGT GATGAGCGCT AGGCGATTCA TC -#GGAGGTAA 6540 - - GGACTCCGTC ATCGCCCAGC TCATCGAGCT TGGACAGCGT CCGATCTGGG AG -#GGAATCGC 6600 - - GATGGCCAGA GCCATCATCG ACGCCCTCAC GTTCTTCGCC AAGTCGACCC AA -#GGCCCGAG 6660 - - CTGGCCGCAT TTGTACAACC GCTTCCCGGC GGTCGAGTTC CTACGACGAA TC -#TGAGAAAG 6720 - - GAGGCGGGGT GAGCCTCAAC AACCACCACC CGGAGCTTGC CCCGTCTCCC CC -#TCACATCA 6780 - - TCGGCCCGTC CTGGCAGAAG ACGGTCGATG GTGAGTGGTA TCTGCCTGAG AA -#GACCCTCG 6840 - - GCTGGGGAGT CCTGAAGTGG CTCTCCGAGT ACGTGAATAC CCCTGGCGGG CA -#TGACGATC 6900 - - CGAACCGTCT GGCGACGTTG ATCGCGCTCT CCGAGGCAGG TCTTCTCGAC AA -#CGAGAACA 6960 - - TGTTCATCCC CACCGACGAG CAGGTACGCC TGGTCCTCTG GTGGTACGCA GT -#AGATGACC 7020 - - AGGGCCAGTA CATCTACCGC GAGGGCGTGA TCCGCCGGCT CAAGGGCTGG GG -#CAAGGATC 7080 - - CGTTCACCGC CGCGCTCTGC TTGGCGGAAC TCTGTGGCCC CGTAGCCTTT TC -#ACACTTCG 7140 - - ACGCCGACGG TAACCCGGTC GGCAAGCCGC GTTCAGCCGC GTGGATCACC GT -#CGCGGCCG 7200 - - TCAGCCAGGA CCAGACGAAG AACACGTTCT CGCTGTTCCC GGTGATGATC AG -#CAAGAAGC 7260 - - TGAAGGCCGA GTACGGCCTG GACGTGAACC GCTTCATCAT CTACTCCGCA GC -#CGGTGGCC 7320 - - GTATTGAGGC AGCGACCTCG AGCCCCGCGT CGATGGAGGG TAACCGCCCG AC -#GTTCGTCG 7380 - - TCCAGAACGA GACGCAGTGG TGGGGCCAAG GCCCCGACGG CAAGGTCAAT GA -#AGGCCACG 7440 - - CGATGGCAGA GGTCATCGAA GGCAACATGA CCAAGGTCGA GGGCTCCCGC AC -#CCTGTCGA 7500 - - TCTGCAACGC CCACATCCCC GGCACCGAGA CGGTCGCCGA GAAGGCATGG GA -#CGAGTACC 7560 - - AGAAGGTCCA GGCAGGCGAC TCTGTCGACA CCGGGATGAT GTACGACGCG CT -#GGAAGCGC 7620 - - CGGCCGACAC CCCGGTCTCC GAGATCCCCC CGCAGAAGGA GGATCCCGAG GG -#ATTCGAGA 7680 - - AGGGCATCGA GAAGCTCCGC GAGGGCCTGC TCATCGCCCG AGGCGACTCC AC -#CTGGCTGC 7740 - - CGATAGACGA CATCATCAAG TCGATTCTGT CGACCAAGAA CCCGATCACC GA -#GTCGCGGC 7800 - - GCAAGTTCCT GAATCAGGTA AACGCCGCTG AGGACTCGTG GCTCTCACCG CA -#GGAATGGA 7860 - - ACCGGTGCCA GGTCGACCTG GCCAAGTACC TGGATAAGCA CGGCAGGGAG TT -#CGCTCCGC 7920 - - TGCAGCGCGG TGACCGGATC ACCCTCGGGT TCGACGGGTC GAAGTCCAAC GA -#CTGGACCG 7980 - - CGCTCGTCGG CTGCCGTGTC AGCGACGGCC TGCTGTTCGT CATCGACATC TG -#GGATCCCC 8040 - - AGAAGTACGG CGGGGAGGTT CCCCGCGAAG ACGTTGACGC CAAGGTCCAT TC -#GGCGTTCG 8100 - - CCCACTACGA CGTGGTGGCG TTCCGCGCCG ACGTGAAGGA GTTCGAGGCG TA -#CGTCGACC 8160 - - AGTGGGGCCG GACCTACAAG AAGAAGCTCA AGGTCAACGC CAGCCCGAAC AA -#CCCGGTGG 8220 - - CGTTCGACAT GCGCGGACAG CAGAAGAGGT TCGCGTTCGA CTGCGAGCGA CT -#CGAGGACG 8280 - - CGGTCCTTGA GGGCGAGGTC TGGCACGACG GCAATCCCGT TCTGCGCCAA CA -#CGTTCTGA 8340 - - ACGCCAAACG ACACCCAACG AACTACGACG CCATCGCGAT TCGCAAGGTC AC -#GAAGGACT 8400 - - CCAGCAAGAA AATCGACGCT GCAGTCTGCG CTGTCCTCGC GTTCGGGGCG AG -#ACAGGACT 8460 - - ACCTCATGAG CAAGAAGGCC CGTAGCGGCC GGGTGGTGAT GGTTCGATGA CA -#GCACCGCT 8520 - - CCCCGGTATG GAGGAGATCG AAGACCCCGC AGTCGTACGA GAAGAGATGA TC -#TCGGCCTT 8580 - - CGAGGATGCT TCCAAGGATC TCGCCAGCAA CACCAGCTAC TACGACGCTG AG -#CGCCGGCC 8640 - - AGAGGCCATC GGCGTCACCG TCCCGAGAGA GATGCAGCAA CTGCTGGCTC AC -#GTCGGATA 8700 - - CCCCAGGCTC TACGTCGACT CAGTCGCCGA GCGCCAGGCC GTCGAGGGTT TC -#CGCCTCGG 8760 - - CGATGCCGAC GAGGCTGACG AAGAGCTGTG GCAGTGGTGG CAGGCCAACA AC -#CTCGACAT 8820 - - CGAGGCACCA CTGGGCTACA CCGACGCTTA CGTTCACGGC CGGTCGTTCA TC -#ACGATCAG 8880 - - CAAGCCAGAC CCGCAGCTCG ACCTGGGTTG GGATCAGAAC GTCCCGATCA TC -#CGCGTCGA 8940 - - GCCGCCCACC CGAATGCACG CCGAGATCGA CCCCCGGATC AACCGGGTGT CC -#AAGGCCAT 9000 - - CCGAGTCGCA TATGACAAGG AGGGCAACGA GATTCAGGCT GCCACGCTGT AC -#ACGCCGAT 9060 - - GGAGACCATC GGCTGGTTCC GCGCTGACGG TGAGTGGGCT GAGTGGTTCA AC -#GTCCCGCA 9120 - - CGGTCTGGGC GTCGTTCCCG TTGTGCCGCT TCCGAACCGG ACCCGGCTCT CG -#GACCTGTA 9180 - - CGGCACCAGT GAGATCACGC CCGAGCTTCG GTCGATGACC GACGCGGCGG CG -#CGCATCCT 9240 - - CATGTTGATG CAGGCGACCG CCGAGCTGAT GGGTGTCCCC CAGCGCCTGA TC -#TTCGGCAT 9300 - - CAAGCCCGAA GAGATCGGCG TCGACTCCGA GACCGGCCAG ACGCTGTTCG AT -#GCGTACCT 9360 - - GGCCCGGATC CTGGCGTTCG AGGACGCTGA GGGCAAGATC CAGCAGTTCT CT -#GCAGCCGA 9420 - - GCTGGCCAAC TTCACCAACG CGCTCGATCA GATCGCCAAA CAGGTCGCTG CG -#TACACGGG 9480 - - ATTGCCTCCC CAGTACCTGA GTACCGCCGC AGACAATCCG GCCTCCGCTG AG -#GCGATCAG 9540 - - GGCCGCTGAG AGCCGACTCA TCAAGAAGGT CGAGCGGAAG AACCTGATGT TC -#GGCGGCGC 9600 - - ATGGGAAGAG GCCATGCGGA TCGCCTACCG GATCATGAAG GGCGGCGACG TT -#CCCCCGGA 9660 - - CATGCTCCGC ATGGAGACCG TCTGGCGAGA CCCGAGCACT CCCACCTACG CG -#GCCAAGGC 9720 - - CGACGCAGCC ACGAAGCTGT ACGGCAACGG CCAGGGTGTC ATCCCGCGTG AA -#CGTGCTCG 9780 - - CATCGACATG GGCTACTCCG TCAAGGAGCG CGAAGAGATG CGCCGATGGG AC -#GAGGAAGA 9840 - - GGCCGCAATG GGTCTCGGCC TGTTGGGCAC GATGGTCGAC GCCGACCCGA CG -#GTCCCAGG 9900 - - CTCCCCGAGC CCCACGGCAC CGCCGAAGCC ACAGCCGGCC ATCGAGTCGT CT -#GGTGGTGA 9960 - - TGCGTGACCG CAGAGGAGTA CGCGGCGGCT CAAGCCGCGA TCACTGCGGG TC -#TTGCCACA 10020 - - TACGTCCAGA GGTTCGCTTC GCTCTTCGTC GGTCCAGCTC TCGCTGTAGG TG -#AGTGGCTG 10080 - - CGACTGCTGC AGGTGCTGTT CCCCGAAATC CAACGGCGGT ATGCAGATGC TG -#CCGCCTTG 10140 - - GGCAGGGACT TCTACGACTC CCAACGCGCA CTACACCACC CAGAGCTGCC CC -#GGAACGAG 10200 - - AGGTTCCGGG GAGAGCTTCG GTGGGAGTGG TTCGTCCAGA ACATGGAGCC CG -#CTCGAAAA 10260 - - GAGATGTCGC AGGCCGACTC TCCGCCGAGT GCGACCTCTA AGTTGGCTCT GG -#CCGCAGTT 10320 - - CGCGAAGTGG AGATGGCAGC ACGCCGACAG ATCATCGGCG CTGTCAAGAA CG -#ATCCGGCC 10380 - - CCGCAGATCG TGCAGGGCTG GGCGAGGGTC GCCACCGGGC GCGAAACATG CG -#CCTGGTGT 10440 - - CTGATGCTCA TCTCACGGGG TGCCGAGCTG AATCACAAGG GCAACTTCGC CT -#ACAGCTCA 10500 - - GCGGAAGCCG CAGGGCTCAA CCTCGATGAC GAGACCGTGA TCGACCTCTG GA -#ACGAGTCC 10560 - - GGTCACGACC TTGAGAAGTT CCGCGAGGAG ACCAGAGAGG ACTTCGAGAA GT -#GGCACGCA 10620 - - GGGTGCGACT GTCTGGTGGT CCCGGTCTTC GATGTGCAGA ACTGGCCCGG AA -#GAGACGCT 10680 - - GCCCTACGGG CGCAGCAACT TTGGATCGAA GCCAGCGACG AAGCTGACGA CC -#TCATTGCG 10740 - - TCAGGCAAGG CCCGCTCCAA GAACAAGAAC ACGGAGACGC TCAACGCGCT CC -#GACGCCGC 10800 - - CTAGCACGCG GCGAAATCAC CATGTCCAAC TACGCCCTCG CTGCGTAGTC CC -#TCGAACCC 10860 - - CAGGTGGGTT CTCTCAACAT GCCCAGGAGG CGAAAACACA TGTCCGACAA CC -#CCACTCCC 10920 - - GAGAGCACCC CAGAGGCCGA GACCCCGGAG GTCGAGAAGC CGATGGAACC GC -#AGGGCAAG 10980 - - GTCTTCGATG AAGCGTACGT TCAGTCGCTT CGCCAGGAGG CTGCAGCCGC TC -#GGGTGGCG 11040 - - AAGAAGGACG CCGTAGAAGC GGCAGAGGCT CGAGTGAAGG CCGAGTACGA GG -#CCAAGCTC 11100 - - GCTGAGCGCG ACACCGCTTA CACCGAACTG CAGAACCAGT TGGGACAGGC GT -#GGATTGAG 11160 - - CTGGAGAAGG TCTACCTCTC TCTCGACGCC AAGGTGCCCA ACGACAAGGT TC -#GGGCGTTT 11220 - - GTCGAGATCC TCGAAGGCAA CGACAGGGAC AGCATCGCTG AGTCAGTGAA GT -#CCCGTCTG 11280 - - GAGCTGGTCG GCGGATTCGG CAACAAGACC CCGAGTCCTG CGTTCGACCC GT -#CTCAGGGT 11340 - - CGCGGCGGTA AGCCGCCGAT CCCGCTGAAC GGTGACCCGA TCCTCGAGGC CA -#TCAAGGCC 11400 - - GCTGTCGGGA TCAAGAAGTA ACCCACCCAA CAGATCTCAA GGAGAGATAA AC -#AATGGCAG 11460 - - TCAACCCTGA CCGCACCACG CCGTTCCTCG GCGTGAACGA CCCCAAGGTC GC -#GCAGACCG 11520 - - GCGACTCGAT GTTCGAGGGC TACCTCGAGC CCGAGCAGGC CCAGGACTAC TT -#CGCCGAAG 11580 - - CGGAGAAGAT CTCCATCGTC CAGCAGTTCG CCCAGAAGAT CCCGATGGGC AC -#GACCGGCC 11640 - - AGAAGATCCC GCACTGGACC GGCGACGTGA GTGCGTCGTG GATCGGTGAA GG -#CGACATGA 11700 - - AGCCCATCAC CAAGGGCAAC ATGACCTCGC AGACCATCGC CCCCCACAAG AT -#CGCGACGA 11760 - - TCTTCGTGGC CTCGGCGGAA ACCGTCCGTG CGAACCCGGC CAACTACCTG GG -#CACCATGC 11820 - - GGACCAAGGT CGCGACCGCC TTCGCGATGG CGTTCGACAA CGCCGCGATC AA -#CGGCACCG 11880 - - ACAGCCCGTT CCCGACCTTC CTAGCGCAGA CCACCAAGGA GGTCTCGCTG GT -#GGACCCGG 11940 - - ACGGCACCGG CTCCAACGCC GACCTCACCG TCTACGACGC GGTCGCCGTC AA -#CGCCCTGT 12000 - - CGCTGTTGGT CAATGCCGGC AAGAAGTGGA CCCACACTCT GCTGGACGAC AT -#CACCGAGC 12060 - - CGATCCTCAA CGGCGCGAAG GACAAGAGCG GTCGCCCGCT GTTCATCGAG TC -#GACCTACA 12120 - - CCGAGGAGAA CAGCCCGTTC CGCCTCGGTC GGATTGTGGC CCGTCCGACC AT -#CCTGAGCG 12180 - - ACCACGTCGC CTCGGGCACG GTCGTCGGCT ACCAGGGTGA CTTCCGCCAG CT -#CGTCTGGG 12240 - - GCCAGGTCGG CGGCCTGTCC TTCGACGTGA CGGATCAGGC GACTCTGAAC CT -#GGGCACCC 12300 - - CCCAGGCTCC GAACTTCGTC TCGCTGTGGC AGCACAACCT CGTCGCAGTC CG -#AGTCGAGG 12360 - - CCGAGTACGC CTTCCACTGC AACGACAAGG ACGCGTTCGT CAAGCTCACG AA -#CGTGGACG 12420 - - CCACCGAAGC CTGATCCAGG CTTGACATCC ACCGGGAGGG GGCTCCTTCG GG -#AGCCCTCT 12480 - - CCTGATGTGG AGCAGGAAGG ACCACATGCG AATCCAGTCC ACCCTCAACG GC -#GGTTTCGC 12540 - - CGAGGTTTCC GAGGAGTTCG CCAAGCAGTT GATCGCCACT GGCGGCTGGA AG -#GTGCCCCG 12600 - - GAAACCGCGC AACACCAAGA CCAAGACCGC TCCTGAGGAG CCCAAGAACG AG -#GAGTAACC 12660 - - CGTGGCCTAC GCGACCGCCG AAGACGTTGT GACGTTGTGG GCCAAGGAGC CT -#GAGCCCGA 12720 - - AGTGATGGCG CTGATCGAGC GCCGGCTCCA GCAGATCGAG CGCATGATCA AG -#CGCCGGAT 12780 - - CCCCGACCTG GACGTGAAAG CCGCTGCGTC GGCGACGTTC CGGGCCGATC TG -#ATCGACAT 12840 - - CGAAGCTGAT GCTGTTCTGC GCCTCGTGCG TAACCCGGAG GGCTACCTCT CG -#GAGACCGA 12900 - - CGGTGCGTAC ACCTATCAGC TCCAGGCCGA CCTGTCGCAA GGCAAGCTCA CC -#ATCCTCGA 12960 - - TGAGGAGTGG GAGATCCTCG GGGTCAACTC CCAGAAGCGC ATGGCGGTCA TC -#GTCCCGAA 13020 - - CGTGGTGATG CCGACGTGAG CGCGAGCGAC CGACACCGCG CCCCGATTGT CT -#ATCCGCCT 13080 - - GGCACTCAGG CGGTTACGCC GGATCGGGTC AACGCGTTTG ACTGCGATCA CG -#AAGCTGAT 13140 - - CCTCCGGTGT GCCGGTGCGT CCACGACTGG CGCATCGAGT GGGGAAACGT CA -#AGAAGGCC 13200 - - ACCGCCAGAT CACGGTCGGC GGTGCTCTGA TGAGCCTCCT CGACACCGGT GC -#CCGGTACC 13260 - - AGACCTGCAT CGTCTACCCC GAAGAGATGG TCATCGACTC CGATGGCAAC AA -#GCGGACCA 13320 - - GGCCGTCGAA TACCGGCATC CCGGCCATCG CACGGTTCCA GGTAGCCAAC CA -#GTCTGGTA 13380 - - CGTCGGCACG ACGTGCTGAG CAGGACAACG AGGGGTTCGA GACCGAGAAG GT -#CTACCGGA 13440 - - TGCGGTTTCC CCGCTCGTTC ACCAAGGAGC ACGGCATCCT CGGGGCCCAG TC -#CCAGATCG 13500 - - AGTGGCGAGA CCAGCGGTGG GCGCTCTTCG GAGACGCCAC CGTCTACGAC TC -#ATCCCCTG 13560 - - CGTTGGCGCG GGTCGACTAC ACGATCAAGA GGTACTGATG GCCAAGGTCT AC -#GCGAACGC 13620 - - GAACAAGGTC GCGGCCCGGT ACGTCGAGAC GAGGGACGCC GTCCGAGACG AG -#CGGAACAA 13680 - - GGTCACCCGT CGAGCCAAAG CCAATCTGGC GCGGCAGAAC TCGACCACCC GC -#ATCACCGA 13740 - - CGAGGGCTAC TTCCCGGCCA CCATCACCGA GCAAGACGGC GATGTCGACT TC -#CACACGAT 13800 - - CCTCAACGCG CCCAACGCGT TGGCGCTTGA GTTCGGCCAC GCGCCGTCTG GC -#TTCTTCGC 13860 - - TGGCACCGAC ACGAAACCAC CGGAGGCCAC TTACATCCTC ACCCGAGCCG CC -#ATCGGCGG 13920 - - CACCGTCTCA TAAGGAGGTC ACATGGCGCG AATGCCTCGC GTCCAGGCAG TA -#GCGGCCCC 13980 - - GATCCTCCGG TCAGACCCCC GACTGGAGGG AGTGACGGTC ACGACATGGG TT -#CCAGACGT 14040 - - GGACTTCCGA GAGTTCCCGA TGATCAACCT CCGCCGCATA GGCGGGACGA GG -#AACCCCAA 14100 - - CGCACCGACG CTGCACACGC TGCCGGTGGT CGAAATGACC GCCTACACCA GA -#GACGGTCT 14160 - - CATCGAGACT GAGGAGCTGT ACGAGACCGC GCTAGAGGTT CTCTACGACG CG -#GTGGAGAA 14220 - - CGGAACACAA ACTCCCGCAG GGTATTTGAC CTCCATCTTC GAGACGATGG GC -#GCCACTCA 14280 - - GTTCAGCTCC CTCTACCAGG ACTCCTGGCG CATCCAGGGT CTGATCAGGC TC -#GGCGTCCG 14340 - - CAGACCGAGA ACCACCCTCT AACCGAAAGG TAAAGCCACA TGGCTGAAAA CG -#ACGACGCA 14400 - - GTGTTGACTG CGGCGGTCGG CTACGTGTAC GTCGGTGCTG CAGGCACCGC TG -#CTCCTACG 14460 - - CCGGCCTTGC TCAAGACCAT CGACCTCAGC AAGCCCGAGA CCTGGACCGG TG -#CTACCGGT 14520 - - TGGACGAGCG TCGGCCACAC CAGCCGAGGC ACGCTCCCTG AGTTCGGCTT CG -#AAGGCGGC 14580 - - GAGTCCGAGG TCAAGGGCTC CTGGCAGAAG AAGAAGCTCC GCGAGATCAC CA -#CCGAGGAT 14640 - - CCCATCGACT ACGTCACGGT CCTACTGCAC CAGTTCGATG AGCAGTCGCT GG -#GTCTGTAC 14700 - - TACGGCCCCA ACGCCTCTGA GACTCCTGGT GTGTTCGGTG TGAAGACCGG CC -#AGACCAAC 14760 - - GAGAAGGCCG TGCTGGTCGT GATCGAAGAC GGCGACATGC GCCTGGGGCA TC -#ACGCCCAC 14820 - - AAGGCTGGAG TTCGCCGCGA CGACGCGATT GAGCTGCCCA TCGATGACCT GG -#CTGCGCTG 14880 - - CCCGTCCGGT TCACCTACCT GGACCACGAA GACGAGCTGC CGTTCTCCTG GA -#TCAACGAA 14940 - - GACCTCTTCA ACGTGCCCGA GGTTCCCGAG GGCTGATCCC AACTTGACAG CC -#ACCCGGCT 15000 - - GTCTACCCCG GAGGGGGAGG TTTCCTTGGC GGGCCTGGCC TCCCCCTCCT CC -#CGCCACTC 15060 - - ACAGACCCGC CGACACTGAA AGGTTCGCCA TGACAAACGT ATTCACCATC GA -#CGCATTCC 15120 - - GCGAAGAGGT CAAGAAGAAG TACGCTCCGG TCCTCATCGG CCTGTCCGAC GA -#TGTGACCG 15180 - - TCGAGCTGAA GCCGCTGCTG AAGCTGGGCC AGAAGGCCCG CGAAGCGGTG GT -#CGAGGTGT 15240 - - TCAAGGAGTT CGCGGACATC CCCGACCTCG AAGAGGACGA CGACGACGAG TT -#GGTCGATG 15300 - - AGTACTCGCT CCAGGTCTGC GACATCATCG CCAAGGCGTT CCGGCTGATC GC -#CACGAAGC 15360 - - CCAAGAAGCT GATCGCCGCC TTGGACGAGG AGCCGGATCC CCGTATCCGC GC -#AGAGCTGT 15420 - - ATGCAGCGGT ACTCAACACC TGGAAGCGAG AGACGCAACT GGGGGAAGCC GC -#GCCCTCGC 15480 - - CGAGCTGATC GACAAGTTCG GCGGGGCGAT CCTCGCAGAC CTGCTCCAGT AC -#TACCGGGT 15540 - - AGACCTGCGC GACCTGTTCC GCGACGAGGA TCCGCTTTCG CCGAGATTCG TT -#CTGTCCCT 15600 - - GGTGCTCTGC CTTCCCAAAG ACGGCGCGTT CTACGCAGAA CGTCGTGGTG GG -#CAGCAGTA 15660 - - CCGGGGCTGG ACCGAGGACC GCTACGCGCT CGCGGACATC TACGACGCCA TC -#CAGGCGGG 15720 - - CAACCACATC CTGCTGCTGG CGAATCGTGA TCCGAAGAAG CCAAAGCCCA AG -#GCACCCAA 15780 - - GTCATACCCG CGTCCCGACG ACCTAGAGAA GACCACACCG AAGCCGGGTT CG -#TTCGCCGC 15840 - - AATGGTCGTG CGAGCGAAGA AGGCGGCTCG AGAGAGAAGG GAAAGGGAGG AG -#GAGAGTGC 15900 - - CGAATAGTGC TGGCGTAGAA GTCGCCCGGA TCTCGGTCAA GGTCAGCCCG AA -#CACCAAGG 15960 - - AGTTCCGCCG GGAACTCAAG ACCGAACTCG AGAAGATCGA GCGGGAGCTT AA -#GGGCGATG 16020 - - TCGAGATCAA CGGTCATCTC GATGCGGCCC AGGCCAAGGC CGACTTCAAG CG -#CATGATGA 16080 - - TGCAGCTCAA GACCGAAGCT GCCAAGGGCG TTCACGTCCC GGTCGACGTA AC -#CGTCGACA 16140 - - AGAAGAGCAA GAAGGGAGGT CTCCTCGGAG GTCTCCTCGG CGGCAGCCGG GG -#GCTCGGAG 16200 - - ATCTAGGCGA TGACGCCGAG AAGGCGTCGT CTCAAGTACA ACACCTTGGC AA -#GTCGTTCC 16260 - - TGGGCCTCAC ACGAGCCGCC TGGATAGGCG TAGGCATCGT CGCCGTAGCA GC -#TCCGCTGG 16320 - - TCGGCATCGT GGCCGGTCTG CTGGCCGGTC TGCCGTCGCT GCTGTCTGCG TT -#CGGAGCCG 16380 - - GCGCTGGCGT AGTCGCGCTC GGCATGGACG GCATCAAGGC AGCCGCCTCG AC -#GCTGGCCC 16440 - - CGACGCTGGA GACGGTCAAG GCCGCTGTCT CCTCGACGTT CCAGCAGGGA CT -#CACCCCGG 16500 - - TGTTCCAGCA GCTCGGCCCG ATGCTGACCG CGATCACCCC CAACCTGCAG AA -#CGTGGCCT 16560 - - CGGGCCTCGT GAACATGGCC GGGTCGATCA CCGACGTGAT CACCCAGGCT CC -#TGGTCTGC 16620 - - AGCAGATCCA GAACATCCTC ACCAAGACCG GAGAGTTCTT CACGGGCCTC GG -#CCCTGTGC 16680 - - TCGCTACCGG CACGCAGGCG TTCCTGACGC TGTCCAACGC CGGCGCGAAC TC -#GTTCGGCA 16740 - - CGCTCCTGGC TCCCCTGCAG GAGTTCACCA ACGGCTTCAA CGACATGGTC AA -#CCGAGTCA 16800 - - CGTCCAACGG CGTGTTCGAG GGTGCCATGC AAGGGCTTTC GCAGACGCTG GG -#CAGCGTCC 16860 - - TCAACCTGTT CAACCGGCTC ATGGAGTCCG GTCTGCAGGC GATGGGACAG CT -#CGGCGGTC 16920 - - CGCTGTCGAC GTTCATCAAC GGGTTCGGAG ATCTCTTCGT CTCGCTGATG CC -#GGCGCTGA 16980 - - CTTCGGTCTC TGGTCTGATC GGCAACGTCC TCGGGACGCT GGGCACACAG CT -#CGCTCCCA 17040 - - TCGTCACGGC GCTCACGCCG GCCTTCCAGA CGCTGGCGAG CACGCTCGGC AC -#GATGCTCA 17100 - - CCGGAGCCCT CCAAGCTCTG GGTCCGATCC TGACTCAGGT CGCTACGTTG AT -#CGGCACGA 17160 - - CGCTGAACAC GGCGCTGCAG GCTCTCCAGC CGATGCTGCC GTCGCTCATG CA -#GAGCTTCC 17220 - - AGCAGATCTC CGACGTACTG GTGACCAGTC TGGCCCCGCA CATCCCGGCG CT -#GGCGACGG 17280 - - CCCTCGGCCA GGTCGCAGGC GCGGTGCTGC AGCTCGCTCC GACGATCATC TC -#GACGTTGG 17340 - - TTCCGGCGTT CGTTCAGTTG GTCCCAAAGG TCGCTGAGCT AGTTCCGACC AT -#CGTCAACC 17400 - - TGGTCCAGTC GTTCGCCAAC CTGATGCCGG TGGTTCTGCC CCTGGCGCAG GC -#TCTGGTCA 17460 - - GCGTTGCTGG CGCGGTGATT CAGGTGGGTG TCTCCATCGG CGGCGCGCTC AT -#CGGCGCGC 17520 - - TGGCGAACCT CACGGAGATC ATCTCCAACG TCATCAAGAA GGTGTCCGAG TG -#GGTCAGCA 17580 - - GCTTCTCCAG CGGAGCCCAG CAGATCGCTG CGAAGGCAGC GGAACTGCCG GG -#GATGATCC 17640 - - AGTCGGCTCT CGCCAACCTG ATGGCCATCG GCCTGCAGGC CGGTAAGGAT CT -#CGTCCAGG 17700 - - GCCTGATCAA CGGCATCGGC GGGATGGTCA GCGCAGCGGT CAACAAGGCC AA -#GGAGCTGG 17760 - - CGTCCAGCGT GGCTGGTGCA GTGAAGGGCT TCCTGGGCAT CGAGTCCCCG TC -#GAAGTTGT 17820 - - TCACCGAGTA CGGCCAGTTC ACCGCCGAGG GATTCGGCAA CGGCATGGAG GC -#AGGGTTCA 17880 - - AGCCCGTCAT CGAACGGGCC AAGGATCTCG CGGCTGAGCT GTCCAGGGCG AT -#GGAGTCGG 17940 - - GCACCGACCC CTCCGGGATT CTCGCTGGGC TGGATCAGAA TGAGCTGAAG CA -#GATGCTGG 18000 - - CGGCTCTCGA AGAGGAGCGC AAGCGACTCA AGGTCGAGAA GAACGGTATC CC -#CAAGGGAG 18060 - - ACAAGGCAGG CCGAGAGGCG CTGCAGAACC AGCTCGACCA GATCCAGGCG CA -#GAAGGACA 18120 - - TCCTGTCCTA CCAGCGTGAC CGCATCAAGA ACGAGTCTGA GTACGGCGAC AT -#GGCCGGCG 18180 - - AAGACCCGTT GGTGAAGGCA GCCTCCGGGC TGATGAGCGC ACCGGTCGAC TT -#CGCGAAAG 18240 - - CGACTGGCAA GCAGTTCCTT TCGGACATCG GCATCAGCGG AGATGGGTTC AT -#CTCGAAGG 18300 - - CCATCACCGA GGGCATCCAG TACATCTTCC AGATCGGCTC TGTCGATGAG GC -#GCTGTCGA 18360 - - TCAAGGACCG CGAGGAGTCG AAGAACGCGC TGTCCGTCGT TGGCCGCTGA CT -#TGACATCC 18420 - - ACCAGGAGGT AAGCATTGAT CACCGACACC ATCGTTGAAC TCGAGGGTGT CA -#ATGGTGAG 18480 - - CGTTTCAACT TGACGACCGG TGACCAGGGT GTGTACCTGG CCACAGACGT GG -#AGGGTTGT 18540 - - TTCTACGACC CTCCCGTCAA GGTCGTTGTT GAAGAGCCGG GGAACTACCC CG -#GCGCTCGC 18600 - - TACTTGTCCC ACCGAGCCCT GAAGCGAGAC ATCGTCTTTG GGGTCGTCAT CC -#TCAACGAC 18660 - - GCGAAGCAGG GGCCGCGCTC CTGGCTGTCG CGAGACTCCG AGTGGCGCAA GG -#CGTGGGCG 18720 - - TTCAACCGCA CCTGCAAGCT CTACGTCACC ACCCCGGACT CCGGTACCCG CT -#ACCTGAAG 18780 - - CTGGCGCTGT TCGAGTCCCC CACCGTCAAG ATGGACACCG ACCCAAGAGG TA -#AACCCCTT 18840 - - GAGGTCACGG TGATGTCGTG CATCGCGTAC GACCCGTTCT GGTACGAGGA CG -#ACAAGGTC 18900 - - TTCTCGGCCA AGACCAAGAC CGACACCCGG TTCGACCCGT CGTTCTGGAC GC -#CGCCGTGG 18960 - - CCGTGGGAGG AACTGCCCAA GGAGACGCTG CGGATCAAGG TCGGCCGCGA GC -#AGGGTGGG 19020 - - CTAAACCCCA CCGACCAGTA CATCTTCCCG AAGTGGACCG TTCCCGGCTC CA -#CCGAGAAG 19080 - - GTGCCGAACT TCCCCTGGCC GTTCCCCCCG AACGTCCCGA TCCCGTGGGA GA -#CAGCACCG 19140 - - TTCACTCAGT TCGTCATCCC GGACTACTCG TTCGAGGATG AGGAGTTCCG CA -#ACCGCCGG 19200 - - CTCAAGACGC CGGGGTTGAT CTACGGCGAG AACTGCGTCA TCGACACCGA CC -#GGCGCGAG 19260 - - GAGCAGATCG CTTCCGAGTC GGGCTCCCCG GTGTGGGCTC GGATGAACGG TG -#TCCGGTTC 19320 - - CGCAACTCGA TCCCGCCCTA CACCGAAGAG GCTGAGTTCG TCATAGACGC AT -#CGGGATGC 19380 - - GCTCCGGGAC AGGTAGTTAC CCTCCGGCTC ACGAGGCCGT GGTCGCGCTG CT -#GGGGGCTA 19440 - - GAGTGAGTGG TCTGACGAGC GTTCGTGAGG CCGAAGATCT CTGGCAGAAG AT -#CCAATTGC 19500 - - GGCGCTGCAA GCGCGAGCAG GAACGGCTCA AGCATCCCGA CGTAGAGCTG CG -#CGATGGCG 19560 - - ACTTCCGCCT GCGCGGCCTG GTCGCTGGCG AGCGGGTGCT CGAGTGGGAG TT -#CATCGAGA 19620 - - ACGAGACTGG CACCTGCACC TTGCAGCTCT CACTGAGCCA TTACCTGGCG AA -#GTGGGTGA 19680 - - TGGACCACCG GGGTCGAGCA AAGCGCAACG TCATCATCAA CATCGAGAAG CA -#AGGCGCTC 19740 - - GATGGACCGG GATGATGGAC CACTACCGGG TCATCAAGAC CGACGCAGGG GA -#CGCCTACA 19800 - - TCGAGATCGT GTTTTTGCAC GACTTCGAGC AGACCAAGCA TATCCGGGTA TG -#GTGCAACC 19860 - - CGTTCCTACG CCCCGAGCTG CAGTTCCCCA AGGTGTGGAT CATCTTCGGG CC -#GGCCAAGT 19920 - - GGTGTTTGCT GGTGACACTG TTCGTCAACC TGCTCAGGCT CGAGACGAGC TT -#GTGGACGC 19980 - - TGCCTGATGA CCCCACGGAC ATCAACGAGT GGATGGGTCC GAGCTTCAAC CC -#AGCAAATT 20040 - - GGCGGAACAT CGTCAAGCCG TTCCCGTTCC TGGCCGACAA CTCACCGGTC AC -#GATGGTGT 20100 - - TCAGCCGGTT CGGGACGTTC TACGACACCG CCAAGAAGAT CCTCGAAGAC CA -#TCAGCTCA 20160 - - CGCTGACGTG TCGTCGGTAC ATCAAGGACC GCGACCCGCA TCCGTTCGAA GA -#TCTCAAGG 20220 - - GGCTCTGGGG AATTGATCCT GTCGAAGACC TGCTGCAGAA GATCCCGCTC CG -#GGACGGCT 20280 - - GCGTGGTCTG GGACATCGAG GACAACTCAG GTTGGGGCAC TCAGACCGCG TT -#CGGCGGTT 20340 - - CGTGGCTGAC CGGGTTCGTC CGAGGGATGG TCCAACTGGC CGGCGACGGC CA -#GGTCGAGG 20400 - - GCGTCGATGT GTTCACCGGG GACTACACGT TCCCAGGCGA GTACTACTCC CC -#CTGGTTCA 20460 - - TGGGCACCAG CCCGATAGCA CCCCACGTCG TGTTCGAAGA AGGACCGCTG AC -#CGGGATCA 20520 - - AGTCGTCGGA GTTCTCGTAC TACGAGGCCA CCGACACCAG CTTCCTGGCT GG -#TGGACAGA 20580 - - GCGCACCTGG CATCAACGAG GGCATCTCGG CCCTGGTGAA CATCGGTGGC GA -#CCTGCTGA 20640 - - CCTCGTTCAT CAACAGCCAG CTCGCCGCGC TCGGCGCGGT CGGTGGAGCG AT -#TGACCTCC 20700 - - CGCCTCTGGG CGGTCTGCTC GATGCGGTGT TGCAGCCTCT GTACTCCGAT GT -#GTTCGGCG 20760 - - CGTTCATGGA AGTTCCGACT CTGCGTGCGA TGGGCATCTC GCTCCCGATC TC -#CGGGCTCG 20820 - - AGGACATCGT CACCGGACTG GGCGACTTCC ACTACTTCGA GAACATGGCC GA -#CGGGGCGA 20880 - - TGAAGGCGTT CACGCTGTCA GCGTTCGCAG CCATCGCATC GCAGATCCAC AA -#GACGAGGG 20940 - - CTCGAACGAC CCACACCCTC AAGGTGTCTG ACGCCGCTCC GTACATCTTC GC -#GCCAAAGC 21000 - - CCTACGGGCA CTGCTGGATC GGAGATCGCG TCGGCACGTC GGTCCTCGGC TA -#CCCGGTCG 21060 - - AGCACCAGTT GTTCGTGGAG CGCATCCGCA AGGTGAAGTA CCGCATCGAC AA -#AGACGGCA 21120 - - TGAAGCCGTT GGAGATCGAG ATCGGTTACC GCGAACCGAA GAACCCAGCA CT -#ACACATCC 21180 - - TCGAAGAGAT CAAGCGCGTC AACGGCGCTC TTGGCACTGC GGGGATTCTC TA -#AACCGAAA 21240 - - GGCACGCCGC ATGATTCCCT CACAAGAGTC TCACAATCCG AACGACCCGC GA -#CAGCACGT 21300 - - CATGTGGGCG CTACGCAATC TCCCGATGAT TGCTGGCGTC GGGGCGATCA CG -#CATCCGGG 21360 - - TTACCTGGCG GATTGGTCAG AGCACTTGTG GAAGTGCGGC TTTCGGCACG TC -#GACTGGCT 21420 - - CCGGGAGCTG GCTGATGAGG ACGGCAACAT CCACGTCAGT CAGCTTCCTG AC -#CAGGAGAT 21480 - - CAAGTTTCAG CAGCCCTTCC GGGGCCAGCG AAGCGACTAC AACAACGCAG CT -#CGATGGGT 21540 - - CGGCAAAGAC GATCCTGACC CAGAGCCCGT GCGTATTCCA GACATTCGCA AG -#CTCACAGA 21600 - - CCAGGAGAAC AGAGCGATGA TCGCGCAGTA CGAACGAGAC GGTTGGATCA AG -#GATGGATC 21660 - - CCCCGGCCCA GCGATAGCCG AGGTCGTGGA GTGACCCCGT TCAACCCAGA CT -#CCATAGGC 21720 - - GACTACGTGA CACTGCTCGG CGTTGCGTTC CTGACCTTCT CGGTTCCCGC AT -#GGTTCACC 21780 - - GGACGAGCAC GCAAGCACAG CAGTGACATC GGCGAAATCA AGGAACAGGT AT -#GTAACACC 21840 - - CACGACACGA ACCTGCGCGA TGACCTCGAC AGCGTCAAGG CAGACATCAG CG -#ACTTGAAA 21900 - - GAGATTGTGT TGCAAGGGTT CCACCAGGTG AACGAGTCGA TCAACCTCGA GC -#GCCGTGAG 21960 - - CGGATCGAAG GAGACCGCCG AAAGGAGGTT GCGTGACCTA CCCCACCAAC CC -#ACTAGAGG 22020 - - CCATCGGCGC TGACGGCGCA TTCGAGATCG GTGGGGGCGA CTGGAGCTTC GG -#CCAGGACT 22080 - - ACACCGAACA GGCCATCCGG GCTCTGTTCA CGATGCCAGC GGTCACGATG GA -#GAACGCTC 22140 - - TCGGCCTGCT CGAAGAGCAC CTGCTGAAGC TGCCTCTGGA GGCGCTGCAG GG -#CTTCAAAG 22200 - - ACATGATCCC GGACTGGGTC GAAGGAGCAT TCGACACGGT CACCGGCGCT GT -#GCAGGCGA 22260 - - TCATGAACGC GCTCCAAGAC GGCCCGCTGT TCCTGAAGTT CGCCGAGTTC CA -#GCTCTTCC 22320 - - TGCAGCGTCT GCTGAACAAC CCGGCCGAGG TCATCGGCGA GATCCCCCAG AC -#GTTGATCG 22380 - - ACGGCCTACA GGACGCGCTC AACACCGTCA ACAACACCAT CCAGACCATC GT -#GGACATGC 22440 - - TCCTGCAGGC GCTGGGCATC ACCCCGGAGG GGGAGCTGAT CGACCGGATC TT -#CGACCTGA 22500 - - GCGATGAGAT GGAGTGGCTG CAGACCGCAG CCTCGAATGC AGCTACCGGC AT -#CCAGGACA 22560 - - CCTGGAACAA GTTCTGGGGA GCCCTCACCG GGCGCGTCCC AGACCAGGAC CA -#GACCGTCG 22620 - - CTGAGCCCGC CGAGCGTATC GGCGAGCTGG CCGGCACCAC GTCTGCTAAC TC -#GTCTGCCA 22680 - - TCGCGGAGCT GCAGCGTCGA CTGGACAACC AGCAGAACGC TGGCGGCGTG GC -#CGGCGGTG 22740 - - ACGACTTCGA GCGACTGAAC ATATCCGGTT GGGACATCAG GTATTCCAAC GG -#ATCCAGCG 22800 - - GCCGAGGGTA CTACCGTGCC GACGGCCACC AACTGGTCTG GATGGACGAA GG -#CAACCAGC 22860 - - AGAACACCGC GACGTTCGTC CGCACCAACC CCGCAGACGA GAAGACAGCC AC -#CGACTACC 22920 - - AGAAGATGAC GTTGGTCGTC GGGACTATCT CCGGTGAGGT ACAGACCGTG TT -#CCCGCCGC 22980 - - AGGGAGGTTC GCACACCCGG CTATGGGTCC GCGTCAACGA CAACGCTCCG AC -#CGTCGGCA 23040 - - TCACCGACGG CGTGTTCGTA GAGATCGGCG GCGTATCGAA GGCCCAGATC GG -#CTACCGCC 23100 - - GCAACGGCAA TGACACGTTC GTCGGATCTA TGGTCGACTG CACCTGGGGT GC -#TGGATCGA 23160 - - TCTTCGCTCT GACCGCCGGC ACGGCCAACG GTGCTGAGAA GTTCGAGGTC TC -#GAAGAACG 23220 - - GCCCCGTGCT GGCCACATGG TCGGACGACG GCGTCGTCTC CGCGATGGGT GC -#GAACTACC 23280 - - GCCGCTGGGG CTGGGAAGGC CAGGCTCGTA ACCGCAACCT CGGCCAGGGC AC -#TCCGAACT 23340 - - CGGTCACCCG AGTGACGATC ACCGACAACG ATCCTACCGG CGCAGGCGGT GG -#AGCTGTCA 23400 - - ACGTCGGAGG AGATGTCGTA GGTGTACTCC CCATAGAGAA CGGAGGCACC GG -#AGCTTCGA 23460 - - CAGCTTCGGC AGCCCGTACC GCTCTCGGAA TCGATGACCT GGTCGAAGAT AT -#GTCCGACG 23520 - - TAGTTCGTGG ATCCGTCGAA GGACTCCCGT TGATACCGAA GATCTGGGTA GG -#AACAGAAG 23580 - - CTCAGTACAC GGCTCTCGCC ACCAAGGATC AGTCCACGCT ATACTTCAGG AC -#CGCTTAAT 23640 - - GACTGGTATC TCGTTGGGTG TCAACGACAT CCGCAACCTC TCGATATTCT TA -#GGCGTCAG 23700 - - CAACAAGATA TTGAAGGTCA GTCTAGGCAC AGAAAAGGTC TGGCCTGCGT TC -#ACCCCGGT 23760 - - GCTGACCACG TTCGCCACGG TCGGCACGTA CACCTACAAC ATCCCCGACG GG -#GCCAAGTT 23820 - - CATCGACGTC ATCCTCCTCG GAGGAGGCGG CGGGGGTAAA GGCATGGCCC TG -#GCTGACGG 23880 - - CTGGGGCAGA GGTGGAGACG CCGGAAGCTG GGCTATCGTC ACTCTCGAAC GC -#GGGGTACA 23940 - - CATCCCGTTG TCGACCAAGA CGATCACCGG GCTCGTCGGA GCTGGAGGCG CA -#GCGGGAGC 24000 - - TGGCTCTGTA TTCTCAGGCA AGGCCGGAGG CCCTGGAGGA AACACCACGG CG -#TCCGCTGT 24060 - - CGGATGGTCA GGTTTGACCG CAACCGGCGG TCCCGGAGGC TCTGTGATCG AC -#ATCCTCAG 24120 - - CGTCGCCGGA AAGTCGCCTG GAGATCGGAC CTACAACGAC CAGCTCTACA TA -#GGCGGCGC 24180 - - ACAACAGAAC TCAGCTGGCG GGAACGGCAA TGCTCCTGGC GGCGGCGGGG CT -#GGTGCCCA 24240 - - GGTCTCCGCA CAGAGCGGCG GTGCTGGCGC TCGCGGCCAG GCGTGGTTCT TC -#GCGTACTG 24300 - - ACAAGAAACC CCCCTCTTTA GGACTCAGTG TCCTTGGGAG GGGGGCTTTT TG -#CGTTTCAG 24360 - - GAGGTCTTGG CCAGCTTGGA CATCGCCTCA GCGATAGCCT CGTCGCGGGC CT -#CAGACGCC 24420 - - ATCTGGTACT TCATCGCCAT CCTAGGAGTC GTGTGACCGA GACGGGCCAT CA -#GCTCCTTG 24480 - - GTCGTCGCAC CTGCCTGAGC GGCGAACGTA GCGCCGACAG CGCGGAGGTC GT -#GGATGCGG 24540 - - AGTTCCGGCC GACCGATCTT GGCGTAGCCA CGCTTCAGCG ACTTGGTGAA CG -#CGGACTTC 24600 - - GACAGCCGGT TGCCCTGCGT CGTGGTCACC AGGAATGCCT CGGGGCCCTT GT -#TCATCTTC 24660 - - GTACGGTCCT TCATGTGCGC TCGGATCATC TCCGCGACGT GAGGCGGAAC CG -#TCACAGGA 24720 - - CGCTTCGACC GGACGGTCTT GGCGTTGCCA ACGACGATCT TGTTCCCCAC GC -#GGGAAGCG 24780 - - CCACGGCGCA CCCGGAGCTT CATCGTCATG CCGTCGTCCA CGATGTCCTT GC -#GGCGAAGC 24840 - - TCGATCAGCT CTCCGAACCG GAGGCTCGTC CACGCCAGGA TGTATGCCGC GA -#TCCGGTAG 24900 - - TGCTCGAAGA TCTCAGCGGC GACGATGTCC AGCTCCTCAG GCGTCAGCGC CT -#CTACGTCG 24960 - - CGCTCATCGG CTGCCTTCTG CTCGATCCGG CACGGGTTCT CTGCGATCAG CT -#TGTCCTCG 25020 - - ACCGCTGTGT TCATCACCGC CCGGAGGACG TTGTAGGCAT GCCGGCGGGC AG -#TCGGGTGC 25080 - - TTCCTACCCA TCCCGGCCCA CCACGCACGC ACCAGAGCTG GCGTCATCTC TG -#TGACCGCC 25140 - - ACTTCACCTA GCACCGGGTA GATGCGGCGC TCCGCGTGCC CGCTGTACAG AT -#CCCTGGTG 25200 - - CCGTCTGCGA GGTCGCGCTC CACGAGCCAC TTCCGGGTGT ACTCCTCCAG CG -#TGATGGCG 25260 - - CTGGCGGCTG CCTTCTTCGC CCGGTCCTGT GGAGGGGTCC AGGTCTCCAT CT -#CGATGAGC 25320 - - CGCTTCTCGC CCGCGAGCCA GGCTTCGGCG TCCATCTTGT TGTCGTAGGT CT -#GCAGCGCG 25380 - - TAGTACCTCA CACCGTCCTG CGGGTTGACG TATGAGGCTT GGATCCTCCC GC -#TGCGCTGA 25440 - - GTCTTCAGCG ATCCCCATCC GCGACGTGCC AACTAGGTCT CCTCTCGTCG TG -#AACAAGGC 25500 - - TACCGGGTTG CAACTCCTGT GCAACTCTCA GGCTTCAACG CGCTTCTACG AC -#CTGCAATT 25560 - - TCTTTCCACT TAGAGGATGC AGCCGAGAGG GGGTAAAAAC CTATCTTGAC CG -#GCCCATAT 25620 - - GTGGTCGGCA GACACCCATT CTTCCAAACT AGCTACGCGG GTTCGATTCC CG -#TCGCCCGC 25680 - - TCCGCTGGTC AGAGGGTGTT TTCGCCCTCT GGCCATTTTT CTTTCCAGGG GT -#CTGCAACT 25740 - - CTTGTGCGAC TCTTCTGACC TGGGCATACG CGGTTGCAAC GCATCCCTGA TC -#TGGCTACT 25800 - - TTCGATGCTG ACAAACGAAT AGAGCCCCCC GCCTGCGCGA ACAGACGAGG GG -#CATTCACA 25860 - - CCAGATTGGA GCTGGTGCAG TGAAGAGAAT AGACCGGGAC AAGGTTGCAC CG -#GGAGTTGC 25920 - - AGCGGTCGGA ACCCTCGCCG TCGGCGGGCT GGCGTTCGCC CTGTCGTTCA CG -#GCTCTCAG 25980 - - CGAGCTGGCT GCGGCCAACG GGGTGGCCCA AGCAGAGATG GTGCCCTTGG TG -#GTCGACGG 26040 - - CCTGACGCTC GTCGCCACGG TCGCCACAGT GGCCCTCAAG CAGAACAGTT GG -#TACGCGTG 26100 - - GTCGCTGCTG ATCCTGTCCA CCGTCGTATC GGTGGCCGGC AACGTGGCAC AC -#GCCTACCC 26160 - - CCACGGCATC ATCGCGATGG TGATCGCTGC GATCCCTCCG CTCTGGCTAC TG -#GCGTCGAC 26220 - - CCACCTAACC GTGATGCTGG CGAAGCAGCA CTCGGAGCAC GCCGAAGTAC CT -#GTCTCGCG 26280 - - GCCAGAACCC GCGCCTCGGG GCCTGGAGCC CGCTGCCGCT TGACTGCGCC CG -#ACCGGGAC 26340 - - AGAAATACAT AGAGAACCTA TGGATGTAGG AGGCACAAAA AAATACCCCC CG -#AGCCAGCC 26400 - - CGAAGGCCAG CCCAGGGGGC ATGGTTCTGC TTCAGTAGAC CTTGCGAGTC CG -#ACCCGAGT 26460 - - TGATCATCGC CATGATGACC CAGACGGGCA ACCACATTCC GCAGGTGATG AG -#CGAAAGCA 26520 - - ACAGGTGCAT CGCGTGGTTC GTCCTGACAG GCATGACAGT GGGCTGCGGC AT -#CGGAGGAG 26580 - - GCGCGACCGG GTACGGCGAG CCCGCGTACC ACTGAGGTCG ATCTTGTTGG GG -#CGGATACT 26640 - - GATTGGTCAT CCCGACAGCC TACTTGCCGA TGGGTCGCAT CAGCTCCTCG AC -#CGACTCGC 26700 - - GCTCCACGCG GATCAGCCGG GGACCGAGCC GAACGGCCTT GAGCCGGCCG TC -#GGCGATGT 26760 - - AGTTGCGGAC GGTCTTGGTG CTGACACCGA GGTAGTCAGC GGTCTCCTGG AT -#GGATGCTC 26820 - - TCGGGGGCAT CAGCGCGGTC CTCCGTGCTT CATCGGTTGT CTCCCGAACC CT -#GGATCACG 26880 - - CCACGATCCT TGCGGCTCTG GAGCTTGTTG AGGTTCCTCT GGGTGACGGT GC -#TCAACCAG 26940 - - ACATCGAGCT GGTTGGCTAG CTGGGCGACG TACCACATCA CGTCTCCGAG TT -#CCGCCTGG 27000 - - AGGTCGTCTC GGTTCTCCTG GGTGATGACA CCGTCTTTAT CCCGGAGGAT TT -#TCTTGACC 27060 - - TTGTTGGCGA TCTCGCCGGC TTCGCCTACG AGACCCATCG TCACGTAGGA GA -#GACCCTCG 27120 - - ATGCTGTCGC AGTCGCCTGC ACCGGGGTAG ATCGCTGTGT CGCTCGCGGC GA -#TCTGGTAG 27180 - - ATGTCGACGT GCATCAGATC ATCACCGGGA ACAACTGGCC ACCGGGCATC TG -#GATGAACA 27240 - - CCGGGACGCT GGGGGTGTAG TCCGACGAAC CCGTGCCGCC CTCACAGGCG GA -#CAGGCTCA 27300 - - GGGTGGCGGC AAGGCCGATG ATGGCTGCTG CGATGGTCTT CTTCATCTGT TG -#CTCCAGTA 27360 - - GCTAAGTTCG GACTCCAGTT CGCGGATACG CTCCTGTAGC CCTTGGTTTT CC -#AGGTACGC 27420 - - CTCGGCGAGG TTGGCCTCGG CGCGGTCACG GGCCTCGTCC TTCGACGTGG CC -#TCATCGAT 27480 - - TGCCTCGTGT AGCCGGCGGA TCAGATCTGG GATGGCACCG TGCAGACCGC AT -#ATGAAGTC 27540 - - GGCGTCTGCC TCGGAGAGGT GGGACGCCAC CAGATCCTTG TCCTGGGTCT CC -#TGGTTGAC 27600 - - CGCCCAGATG ACGTGATCCT CTAGCCCGTG GTCGGTCTCG CAGATAGAAG GC -#GGTTCTAC 27660 - - CTCCTCTGGC ATCCAGTAAG TCTTCTCAGC CCCGGTGGAC TTCGCCCACT GC -#TGGTAGAG 27720 - - GATGTCGAAG AACTCGTGGT CCTGTTCGTC GGCGGTAATC ACAGATCGTC CT -#CTTCATCC 27780 - - CATTCGTCGT AGTAACACGT ACAGCCGCAG CAGGTGCAGC AGCCGCACTC GT -#AGGTGCCG 27840 - - TAGTCGTAGT CATCCCAGTC GTCTTCGTCC ATCTAGCTGT ACTCCTTCAT GA -#TTCGGTCG 27900 - - AACGCACGCG TCTGCACGCG CATCTCCAGG TCGACCGTTC GCTTCAACCA CG -#CCCATTCG 27960 - - CCGTCGTGGT TGATCTCCCA CTGGCTCTTG AATGTCGCTG TCTCAACGAG GA -#ACTCGACA 28020 - - GTCAACGTGT GCAGTCCGTT GTTGCTGGGC TGGAATCCGA TACCGTCCTC AG -#CGATGTAC 28080 - - CAGGGCAACT CCTGGCCGTC GAAGTAGACG GCCTTGTCGG TCACCAGTAC TT -#CAGGGAAG 28140 - - GTGTGCTCGG TCAACGGCGT CCCAGGTATG GGATGACGCT GGCCCGGAAC TC -#AAGGAACA 28200 - - CCATGTTGTC CGGGCAGTCC TCGGGGACGT TGTCGGGGCG TTCGGCGGTG TA -#GACGCCGA 28260 - - TCTCGTTGCC CTCCAGGGTT CCAAGCTCGT TGAGCTTGTA GATCGCCAGA CC -#CATCAGCT 28320 - - CTTCATCGAG ACCGTTCGGT GCTGGCAGTA CAACTTTGGC TTGTGGCATT AG -#CCCTCCCT 28380 - - CGGAATTACG TATGCGCTGA ACTCGACGGC CGTAATGCCG TCTGGCAGTT GG -#AATCCGAA 28440 - - CCGCTCTTCG AACTCCTCGT TGGTGATGGG GCCGTACTCG AAGGTTCCGG GC -#ACTACCTC 28500 - - GCCCTCCCCC TCGATCAGGA GGTACGCACC GGCGGCGTAC ACCTCCTCGT CG -#TTCGGCCA 28560 - - TCCGACTACG GTCCCGAGGA CCGTGAACTT CCTCGGCTCC ATCAGGGCAC GT -#CCACTTCG 28620 - - TTGATGAGGA ACCGCATCGG AGGTGGAGTG AGCATTGCCT CGGCTATGGC GA -#TGAGGGCG 28680 - - TTCAACTGAC CCTTCAGCAG CTTCTCCTCG TCGCCTGCGG GAAGGTGGCG CA -#CTCGGCGC 28740 - - TCCATCTCCT TGGCGCGTTC CAGATATTCG GTGGCTGTCA AGTTGTCCTC CT -#TAGTAATC 28800 - - AGCGCCGTAG AGCGAACCCC ACGAACGCTT TCCGACCTCG GGGTCGGTGC CA -#ACCAGCAC 28860 - - CGGACCCATC TGTTCTTGCA TCAGGTGGCC AATGTGTGCA GCGGCTCTCT CA -#GCCTCTGA 28920 - - GGCGGGCAGA GACGCGACGA TCTCGTCGTG GATAGGCAAC CGTAGGTACG GG -#GTGTATCC 28980 - - GGCCTCGTGG AGGCGAATCA GAGCCCGACA GGTCACGTCC CGCGACGACG AC -#TGGATCAT 29040 - - GTAGTTCAGC GCGGAGTATG TCCGCGAGCT GTCCACCGGC AGCCGCCGGC CC -#ATCGCGTT 29100 - - GACGATGTAG CCGTTGCGGC CAGCTTCCAT CGCCAGCTTC TTGCTCAGCC GC -#TCCACACC 29160 - - GGGGTATGTC GCAGAGAACG CCTCATGAAC TCGCTTGGCC ACAGGGATCG AG -#ATCCCCAC 29220 - - TGCCTCAGCG AGAGCCTTCG CCCCACCGCC GTAGACCTTC TGAAAGTTGG CG -#GTCTTCCC 29280 - - AACCTTTCGC GGCACCTGGG CTGCGTCAGC GGTCATCTGG TGGAGGTCCG CA -#CCGTTCTC 29340 - - GAATGCCTCG ATCATGTTGC GGTCGCCCGA CAGCGCCGCC AGGACGCGAA GC -#TCCTGCGC 29400 - - CTGGTAGTCG ACTGAGGCCA TCACATCGCC TGGCTCAGCG ATGAAGCATC GC -#CGCACGAT 29460 - - CCAGTCCGAC GACGGCAGCG TCTGCGCCGG GATGCCGGTG ATCGACATGC GC -#GAGGTCCG 29520 - - CGCCTGCAGT GGGTTGATGA ACGTGTGGCA GCGGTCCTCA GAGTCCCTGG TG -#TCGATGAA 29580 - - CTTCTGGACC CAGGTCTTCC GCCACTTCCC CAGCTTCTTA GCCTCCTGAG CG -#ATGGCGGC 29640 - - AAGCTCGTTG CCATCTTCGA CCAGCTTGTC GAGCAGAGCC GCGTTGACCT GG -#CGCTTGCC 29700 - - AGTCTCGGTG CGACCGGTGA TCTTGACGCC CATCTCCTCA AGCCCCTCGG CC -#AGATCCTC 29760 - - GGTCGAGTTG ACCTTCTCCA CGCCGTACTC GGTGAAAGCG ATTGCCTCCC AG -#ACCTCCTG 29820 - - ATCGGCCAAC CACTTCTCGG CGAGCGACCG CGAGTACTCC ACATCGAGCA GG -#AAGCCCTG 29880 - - CCTGTCGATG TAGCTGCAGA TCTCACTGAT CTTGTGCTCG TACGGCACCA GC -#GACCGACT 29940 - - CACGTCGGGC ACCAACGGTG TCAGGCTCTT GCAGACCCTC GCGGTGAAGA TC -#GTGTCCAT 30000 - - CCCGGCGTAC AGCAGGTACT CCGGGTGGAA CAGGTCGATG GTCGACCAGA TC -#TTGGCCTT 30060 - - GGTCGTCTTG TGCTCGGCGG CTAGCTTGGC CATGAGCTTC TTGACGTTCT CG -#GCCTGGTC 30120 - - CTCGGAGATG AACTTCGCGA TCAGCTCTTC GAGCGAGTGC CCGAACCCGC CG -#GCCTCGAA 30180 - - GGGCCGGGGG TCCACCAGCT TCGCCAGGAT CTGCGTGTCA AGCACGCGGG GC -#CACAGACC 30240 - - CTCCATCTCG ATCCCGAAGC ACTGGTCGAG CACCTGGAGG TCGAAGGAGG CG -#TTCTGGAG 30300 - - CACCATGCGC TTGAGAGCGC CGATGGCGAT CCGCACGTCC TCGATGAACA CG -#TCTCCCAG 30360 - - CTCCACCGGC ACCACCCAGG CTTCGTCCTG AGTACCGAAC TGGACGAGGC GG -#CACTCGAA 30420 - - GGTGTCGCTG TAGATGTCCA GCCCGGTGGT CTCAGTGTCG ACGGCGAGGC AG -#TTCAGGTG 30480 - - AGCCCGGATG AAGTTGCGGA AGCCTTCCAG ATCCTCTGGG GTTTCAACGA CG -#TTGACGGT 30540 - - GACGAGGTCT CCCTGAACCT CATGCCGCAG CTCGATCAAA ATGCTCTCCT AC -#TGGAAGTA 30600 - - CTGAGGCGGA ATCCAGGTGG CTGAGGCCAT CTCCTTGATG GCCTGCTGCA TG -#GCCGCTTC 30660 - - GAACGGACAG TCCGGGTCGA TGTCCGGCTT GTAATGGGTG ACGATGATCC GG -#CTGTTGCC 30720 - - GCCGAAGTCG TGGCTGACCA AGCCCTTTGG GGGCAGCTTC TTCAGCGCCT TG -#ATCAGTTC 30780 - - CTCAACCGTG GTCCCGGTAG GGGCCTTGCC GTCAGGCAAT GCCTCCCCTC CG -#TACGGCAC 30840 - - GTCCAATGGG ATCGTGTACC GCTCAACGTC TTTGATCTTC ATCGAGCCTC TT -#CCTCTTCG 30900 - - ACTACCTCGT CTACCCGGCG GAATAACTCC GCTAGTTCTG CGGGTAGCAA TA -#CTGGGTAC 30960 - - TTCTCTCGGG CTTCCTGCAT CGCTACCGCG ATCCCAATCA GGGCAGCGAG CA -#GTTCATTG 31020 - - ACGGAGTACG CCAACAGCTC TTCGCGGATC TCTTCTCGGG TCATTAGTGG TA -#GATCCCCC 31080 - - GGACGGTGCG CGAGATCGTG GCAGGGTTCA CGCCGTAGTT CTCGGCGAGA TC -#CTTCTGCT 31140 - - TCATACCGCC CAGGTACGCC TGGCGGATGT CCTTGACCTC GCGCTCGGTG AG -#CTTCTTGC 31200 - - GGTTCGGCCG GCTCGGGCCG GTCTCAGGCT TGACCTGAGC CAGCGCCTTG CC -#GAACAGCT 31260 - - CGTTCTGCGT CCGCTGCTTG ATCGCGTACC GACGGTTCGC TGCAAGCACC TC -#GTTGAGCC 31320 - - GCTGGGACAA CTTGACATTG GCCTCACGCA CTACCTCGAC CTCTCCGAGC AA -#GTTCGTGA 31380 - - TCCGGTAGTC CTTGTCCTGG TTCTCGATGG CCAACCGGTT GTTCTCCTCG GA -#AAGCATCG 31440 - - AGACCTTGTA TTGCGCCTCT CCCAGCGCAG CTTTCAGGTG CTTCTTCCTC AT -#TCAGCGCC 31500 - - CCTCTCTCGG CGGAACTGTT CGTACTCGTC TTCGGTCATG TAGTAGTAGT AG -#TCAACGAC 31560 - - CTTGTCCCAG TTGAAGGTTC GGGACGTGCC GTCATCGAAC GCGATGATCA GG -#ACACCCTC 31620 - - TTGGGTGTCT AGGATCGGCT CGCCAGCCAC GACGTGGAAG CGGTCCTCGA GG -#GTCACCGC 31680 - - AGTCGCTCTG CGTGCCATGT CAGTTCCTCT CAGTAGCTGT AGGGGACATC CG -#GGATGTCC 31740 - - TGGTAGGTGT TGGGTGCGAT CTGTCGGAGC TGCCGAAGCA ATTCCCCTGC CA -#GCTCACGG 31800 - - ATCTCGGCAT CCGCGGCCTC GTGCCAGCGG GCCTTGATGA CGTACCGCCA CG -#CCCGATGG 31860 - - TTGCCCGTGA CGACCATCGG TGAGTTCGTC ATGTTCGGCA GGACAGCTCG CG -#CTGCCTCG 31920 - - CGGGCCTGCT TGCGCGGCAA GCCCCGGTCA GCCAGCCGGT TGACGATGTG TT -#CGTAGACA 31980 - - GCGTCAATCT CAGAGCTGAC GGACTCCATG ATGTGGACGA GGTCGTCTCG GT -#CGTCGGGG 32040 - - TGGAGCTTGA ACAGAGCCGG GGGCAGATGG ATGCCAAGGT CGGTCGGATC CA -#CATATCGC 32100 - - TGAGACACCA CCGAGAAGCT CAAGTGACGG TGACGCTCCA GCTCGGTCAG CA -#CCGACCTG 32160 - - CTGGCCTCGA TGTAGAACGT CGCCGAGGCG TGCTCGAACA CGCTCTCGTG GC -#CCAGATCG 32220 - - ATGATGTGGT TGAGGTAGTC CTCGTTCTCG GCAGTTGCCG GGTTCGGTCG GT -#GGAACGAC 32280 - - CGGTAGCAGT TCCGGCCCGC GAACTCGGCC AGCTCGTCGG CATCGAAGTC GC -#CGAAGTAG 32340 - - GGATCTTCGT CCTTGGATTC TTCGAAGTCA TCGACCTCGA ATCCGATGTC CC -#GCAACGCA 32400 - - CCCGGATCGA TCTCGGTGGC AGCGATCAGT TTGGCTTTCA TACTCTCCGC TC -#AGAGTTGG 32460 - - TGGAACGAGG TCAGCCAGGG GGCAGCGAAG CCCTTCTACA GCTCCCCTTG GC -#TCGTTACC 32520 - - GGCTTCTCGA CCTCGGTGGA TGTCAAGTAG TCGAGATGAC TACTTCTTGT CG -#GGCCATTG 32580 - - CGCGTCACAC TGCTGATCGC GAGGTGCGGT GCAGGAGAAC AGCGCGTACG GC -#TTGCCCGT 32640 - - CTTCTTCGAG ACGCCCGACT TGTAGACCAT CTCGCCGTGC TGGCAGTACC GC -#TTCTCGCC 32700 - - ACCAGGCGCT TCCTGAGCTG CCTGCGGGGC GCGAGACTGC TGCTGGCCAC CG -#CCGCCGCC 32760 - - GTTGGCCGGC GCGGATCCAC CGGAGCCTGC GTAGTGGCCT GCGATCTGCT GG -#ACCTTGTC 32820 - - CATCAGCGCC TTGAACTCGG CGGTGTTGAC CTTGGCCAGC ACGTCGGCCG GG -#TCCGCACC 32880 - - CTTCACGACC ACCCACGGGT CGCTGTACTG ACCGGCGAAC TTGAACGTGG CC -#GACACCCC 32940 - - ATCGGTGGAG TGCTGGACCG CCATCGAGTC GCGCACAGCA GCCGAGGCCG TC -#GTCACCGT 33000 - - CGCCGACGGC GCGGTCTCAG GCTCAGGAGC CGGGGCCGGC TCGGGCTGGG CA -#GGGGCGGT 33060 - - GCTCCACGGA TCGTCGTAGG ACAACTGGTT ACCTTTCACT TAATGGGGCA TG -#CGCCGTTG 33120 - - GCGCACTCTT CATCGACACC GTCTTCGACG GCTTTGGCCG CAGCAGATTC GT -#ACTGCTGC 33180 - - TTGGTGATTC GCTCGTACGG AGCCTGCGGG AAGCTGGACT CCGGGAAGAT CG -#TGGAGCCC 33240 - - TTGATGAGCC CCGCGAACCT CTTGAGATCG GCTGCGACAT CCTCGGCCTC GT -#AGGCGTCT 33300 - - GGATGGACGT TGGCGGTGAA CGACACCGCG TTGTCAGCCC AGCACATCTG GT -#AGAGCGCC 33360 - - TGGAACGCCA GGAGCTGGTG GAGGGTCAAC TCGTCGGCTG ACTCAACGAT CT -#CCTCGTCC 33420 - - CAACCGAGTT CCTCGACAGC CTGGACCAAC GTGTCCTTGG TCGGGATCGA AA -#CCACCTCG 33480 - - GTGTTCGGAG CGAAGAGATC CTTCTCGATC TCGTAACCCT CGGCTGCCAA CC -#TCCGCAGC 33540 - - TCGGCCATGT CGCTGTTGAG GTTGAACCGC ACACGCCGGA TGAAGTACCG CG -#AGAAGATC 33600 - - GGGTGGATCC CCTCGGAGAC TCCTGGCATC TTCGCCACCG TGCCTGTGGG AG -#CGATGGTT 33660 - - CGCTTCTTCA CCGGGACAGG GATCCTCAGA TCATGGGCGA ACCGTTCGGC CT -#CTGAGTCG 33720 - - ACCTCAGCGG CCATCTCCCG CAAGAACTGG GTGAACCGCT TATCTCCGGG TG -#CCTCGGAG 33780 - - TACCTGCTAC CTGTGAGGGC CAAATAGGAG GCAACTCCGA GATGACCCAC GC -#CGATGCGA 33840 - - CGGTTTCGGT CCAGAACCTC CCGGCTCTTC GGGTCGGCCA CTTCCGAGAA CG -#TCGCCCGG 33900 - - ATCAGGAATC TCGTCATCAG ACGATGCGCC CGGATCAGGT CGAGGTAGTC GG -#TCTTGCCG 33960 - - GCCGGCGTCA CGAACGCCGC CAGGTTGATG TGGCCGAGGT TGCACGGCTC CC -#ACGGTTCG 34020 - - AGAGTGATCT CGCCGCATGG GTTGGTGCAG ACCACCCGGT TGGGCTCACC GA -#CGTTGGAC 34080 - - AGTGACGAGT CCCACATCCC CGGCTCTCCG TTGCGTACGG CTCCCTCGGA GA -#GTGCCTTG 34140 - - AGCACTCGGT GGGCTCGCTT CTGCTTGGGC ATGTCCTCGC GGGCGACCGC GA -#AGCTGCCG 34200 - - TAGCCCTCCT TGGCCAGACG CCAGAACTCG TCGTCAACCT CGACCGAGAT GT -#TCGTCGTC 34260 - - CAGTGCTCGC CCGTGCTCGC CTTGATGTTG ATGAACTTGT CGATCTGGTA GT -#CGTCCCAG 34320 - - TGCATCATCG ACATCCGCGC CGACCGGCGC ACACCGCCGG CCACAACACA CT -#GAGCGATG 34380 - - GCGTGGTCGA CCTCCATCGC GGCGATGCCG TCGAGCGTGA TCCCTGCGTA CT -#CCGAGAAG 34440 - - ATGTTGGCGA CCTTCTGCAG CATCACAGCG AACGGCAGCG GGCCGCTGGC CA -#CTCCACCG 34500 - - AACGTCTTGA GCTTGGCCCC TTGCGGCCGG ATGCGGCTCA CGTCGTACAC CC -#GCTGGTAG 34560 - - TGGACCGTGC CGGGTCGGTA GTGCGTGTCG ATCAGATCGA CCAGCGCAGC AG -#CCCAGCCC 34620 - - TCTCGTGAGT CCTCGATGGC GTAGGCACCG GCCCAGTCGT GGCTGTAGTG CT -#CCGACAGA 34680 - - ATGCCTACAT CCTTCATCGC CTGGTAGTCG ACATGCTCTG GATCACAGAC GA -#TCTCGACC 34740 - - CGCAGGGGGT TTACGACCTC GGGGTAGCCT TCGAGGTAGT GGTTCGAGTA GT -#TCGCCCCG 34800 - - ACTCCCCCGC CCTCCATCAG GCGCATGAAC GTGAACTGGA AGTGGTCCGA GA -#TCTTCTCG 34860 - - GGCCAGCCAG CTACCCAGCA GTTGAAGAGG TGCTGCGCGT TCTTGACCCC CG -#AGGCCCAC 34920 - - AGATGCCGAC CTGCCGGCAG CACCTTGAAC TTGGTCATCA GACGAACGAG AT -#CTTCTCGC 34980 - - TCTCCTTCCA ACATATGTCG CCGGTCGACA AGAGCAAGAT TGCCGTCCAC GA -#CCCTCTCG 35040 - - ACCGTTTCCG GCCAGGTTTC CTTCGAGCCG TCAGGCTTGG TCCTGGCGTA GG -#TTCGGTTG 35100 - - TAAACGAGTT CACCGGTTGG TCCCCAAGGG ATTTCGTCAG TCAACTACTT CC -#TCTCAGTC 35160 - - AGTTCGTATC GCTTGAAATA GGCGTCGGCA GAGTCGCCGC CAGAGAACGA GA -#CCCCGTAC 35220 - - TCGACCGGGC CTGCACCACG CACCTCGCAG GTAACGACGC CCTTCCTTCC CC -#GGAACATC 35280 - - GGCCAGGTTC CCTTGGAGGG GTGCTTGGTC TCGTCCCGCT GGACGATGAC CT -#TGGTGCCC 35340 - - TTCTTCATGC CGACTTCCGT TCTCCGTAGC CGGGAGTGAA GCAACCCCCG AC -#GTACAGCT 35400 - - CGAGATCTTC TTGCGACCAG TTCTCCAGTC GCATCGGCGG CTGGTGCGGG AA -#CAGCTCCG 35460 - - GGAACACCTC GGCCCGGTAC AGCTCCGAAC CGGGCATCCC GTTGAACGTC GG -#ATCAAGAA 35520 - - TGTTGTGCAT GGCACCTCCC TCCCAAGAAC TCGGAGATCG GCGGCTCGTA GA -#GGTAGCCA 35580 - - TCGCGCAGCT CGGGGTTCTC GATGAGCATG ATCGCGATGT TCGCTGTGGG GT -#CAGAGTGC 35640 - - CCATCCCCCT GCGACTTTCG GATGTCTGGG AAGATAGCGT GCTTGCTGCC CG -#GACCATCC 35700 - - TTGACGATGA CCTTGCCCTT GTCGTCCTTC TCCACGCCAG CCGTGATCGC GA -#TGATGTTG 35760 - - ACGTGCTCGG TCAGCGACTT GTGAGCGCGG AACAACCGGT TCTGCCCGCT CT -#TATCCTTC 35820 - - GGGGAGATCC CGTCGGTGTA GCGGCTCCTG ATCGCCTCTG CATAGCCCCC GT -#TCTGAGCG 35880 - - TCCAGAGCCT TCATCGCCAG CGGGAGGATG TCGACCAGGT ACCGATTGGT CG -#ACTCCCCC 35940 - - TGCAGAGCCT CTTTGACGTT CTCGGACGAG TAGTGGCTGC GCTCCTGGAA CA -#AGTCGCGG 36000 - - GCCTTGGCCG CTCCCGACAG GATGTTGCGA ACCTGATTGC GTACGTAGTG AA -#CTGCCTCA 36060 - - CCACGGTGCA AGCTCTCCAG CGTCTTCTGG ATGTACGGGC TCTCGAGGTA CC -#AGACCCAC 36120 - - AGCTCTTGGA TGATCTCCTC GGCTGTCAGG TTGGTCTCCC AACCGATCAG CG -#CCTTCCGG 36180 - - GTGGCCCTGC TGAACAGCTT GCTGATGTCG TCGGTCAAGG CATCACCTTT CG -#TAGGTACT 36240 - - CCTCCCGGTC CAATCGGCGG TCGAGGTGTC GAGTGACCTC CTCCGCGAAG AC -#CTCGCGGA 36300 - - CTTCGCTGGA GGTGATCTGG CGCGAACGTG CGTTCTTGTG CAGGTACGGC AG -#CTTGGTGG 36360 - - CTGTCAAGTT CTAGACCTCC CAGACTCGGC CGTCGACCGA GAACCGGCCT CC -#GACAATCG 36420 - - GAACAAGCTC AGGCTTGACG TGCTGGCCGT CGACCGTCAG CAGAGCAAAA CC -#ACTCTGCC 36480 - - AGTTGGCTGT TGCACCCTTG AGGTACTGAG CTAGCTTCAT GTTCATCAGG TT -#GCCGACCT 36540 - - CCATCGACCA CAGCACCTTC TGGTTGCCGC CGTAGCCCAG CGTGTGTGGC TT -#GATGCCCT 36600 - - GGCGGTGGGT GTGTCCGATG ATCACCGACG TGCCGAACCG CATCATCGCG TT -#GTACGCGG 36660 - - TGTCAGCGGA CTTCTGCGTC ACCCGGACCC CACCACGGTG GCCGTGGGTG GA -#GATCCAGC 36720 - - CTGGAGCGAT CTTGTAGAAC TCAGGCAGCA CGTCAACACC GAACCCGTCG AA -#GTCCAGCA 36780 - - GGTTCTGGAA CTGGAACGAG CTGACGTACT CGACCAGCGC CGGGGCGAAC TG -#GTGCAGGT 36840 - - AGTCGACTGG CCGGCGGTCG TGGTTGCCCT CGTGGACACC AACCGGGCCG TC -#GTAGACCT 36900 - - GGCGCAGCGG CTCCAGGAAC CGCCGCTTGC ACTGCTCGGA GTCGGGCTTG AT -#CCGCTGAG 36960 - - CGAACTCTTC CTTGGTGCCC TTGGTCCACC GAGACGGGCT CGGGTAGTCC AT -#CAGGTCAC 37020 - - CGATGTGGAC GACCTCGTCA GGCTGGGTGT CCCCGATGTA GCCGATGACC GC -#CTTCAACT 37080 - - GCTTGCGATC ATCGAACGGA ATCTGGGTGT CCGAGATGAC GACGATGCGC TT -#GCTCACTC 37140 - - AGCGACCTCG GTGAAGGGGC CCCGCATACG TTCCTCGTGG GAGCTGGCGT TG -#CCTCCTGA 37200 - - CCAGCGTCGC TTGCCCACCT TGGTGTGGTG CAACCCGTTG GGGTAGTAGA TC -#CACTTCAC 37260 - - TCCTGTGGCG TTGGTGACGG TCTTCACATC GGCAGGAACG TCCAGCAAGG TG -#TCCCACTG 37320 - - GCGAGGCCCC TTGGGATACC GCTCGTCCTC GGGGAGCTGC ATCTTCTCCA GA -#ACGCCTGC 37380 - - GTAACCGGCG ATGTCGACCA CCGTGTCCTG GTGGTAGCCG TTCTCCATGA AC -#CGGGCGAT 37440 - - CTTCAGCAGG ATCATCATGA CGGCCACGTC CTCCGGGGTG AACTCGACGC CG -#CGCTTGTA 37500 - - CGCGCCCCAC AGGGTCGCGA TGCGTTCGTG GTTCTCCTTG GCGTCCCCGT AG -#TCCTGGGC 37560 - - TCGCTGTCCG TTGATGATCT CTTCGGCGGT GGTCAGAATG CTCACAGTCC AG -#TCTCCGAT 37620 - - GCGGTGTAGT AGTCGATCAG CTCATCGAGC TGGTCCGGTT GATAGCCGAG GA -#TCGGCTTG 37680 - - TGGGTGTCAG TGACGACGAC GGGAACCGAC ATCGCGTTGA GCACCTTGGT GA -#CGTAGTCG 37740 - - TACGCCTCCG AGTTGGCCGT GACATCGACT GCGTCGAAGT CGATCCCGGC AG -#CCGTCAGC 37800 - - TTGTCTTTGA CTCGCTCGCA TGGCTTGCAG CCGGGACGGG TGTACACCGT GA -#CCGGCGCG 37860 - - AACAGCGTTC TCACGTGAGC ACCATCCCAG TCGATGTATC GGTCTCCATA CA -#TCAGATCC 37920 - - TTTCCAGCAG AGCAGCTTTG CCCTGCGATG TGACTAGTGA GTTGACATCC TC -#GCCTTCTG 37980 - - GCATCGGGAT GATTCGGGCG TTCGGCAGCG TCTTCGCCAC CGACCGGGCG AA -#CTCCATAC 38040 - - CGGCGTCGTC GCCGTCGGCC AGGATGTTCA CGTTGCGGTA GCCCAGGAAC AG -#CTCTCGGA 38100 - - AGTACGGCTT CCACTTCTGG GCTCCGCTGA GCCCCACCGT CGGCAGCCCA CA -#CAGCTCGG 38160 - - CGGTGATCGT GTCGAGTTCT CCCTCGCAGA TCGCCATGTC CTTGCTGTAT TT -#GGTCAGCG 38220 - - CGTAGGTGTT GTAGAGCCGG TCCTTCTCCC CTGGCATCGA CAGGTACTTC GG -#TGTGCCAC 38280 - - CGTCGATTCG GCGATACCGG ATCGCAGCTA CCGTCCAGTG ACGCCAGGGC GA -#CCACCGCA 38340 - - TATACGGAAT CGCCAGGCAG CCCCGGTACA TCTCATGTCC AGGGAGTGGG TC -#GTCCACGA 38400 - - ATCCCAGACC GAACCGGCTT AGTTCCGCTC GGCCGGCCAG CCCGCGACTC GC -#CAAATACT 38460 - - CGTCGGCTGG GCTTCCGGGC AGGCTTTCTC TGTACCGGGA CGTTGCCTCC CA -#CAGATAGG 38520 - - TTCTCTGCGA TTCGCTTAGC CTCTGCAAAT GTCACCTCCT CTTCGTGACG AA -#TGATCGAG 38580 - - ATCACGTCTC CACGGACCCC GCAGGCCATG CAGTTGTAGC CCTGTAGGTC GT -#AACTGACT 38640 - - GCGGCAGACG GCGTTTCGTC GCCGTGGAAG GGGCACAGGC ACTTGTTCCA CT -#CGTGGTGG 38700 - - TCAGGTGGTG GTTCCCAATC CGGGTGGTAG CGAAGAATCG CCCTCGCGAT GG -#GCGAGTCG 38760 - - TTCATTCGTC CTCGTCAAGC TCCTCGGGAG AGAGCCCTTC GAAGATCCCG TT -#CAGGACGG 38820 - - CGGCGAAGCC CTCGCCGGTC TCCGCTGCGT CGAGCATCTC TGCAATCGTC TT -#TGCCATGT 38880 - - TTCCTCCTGG TGGATGTCAA GTTCGAGACA GCTTGTCAGC CTCGACTGGA GC -#GATGCGCT 38940 - - CCCCGATGAC TTGGACGGCC GGCGGGTTCA GCAGGTACTC GATGGCCCGT TT -#GAAGAACT 39000 - - CGATGCAGTC CCTCGCCCAG CCCAGCGTGT ACTTGTTGCA CATCGTGCAG AG -#CAACCCTC 39060 - - GGACGATGCC TGTCTTGTGA TCGTGGTCGA CCGACAGGCG CTTCTTCTTA CC -#GTTGGCTC 39120 - - GCTGGCAGAT GTAGCACCGA CCACCTTGGA ACTCGTAGAT CTGCCAATAC TC -#ATCGCCGG 39180 - - TGATGCCGTA GGTGGCCAGG ATCCGGGTCT CCCAGCTCGT AGAGCTGCGA GC -#CGTCCTGA 39240 - - ACTCTCGGTG ATGAGTAGCG CATCGTGGCC CTGGATACTT GGCGTCTCGC GT -#GAGCGGGA 39300 - - GCCCCTGTGC GACACAGTCT TTGCAAGGCT TCCGCTTGTG CTTACGGTTC TG -#CACCCGGT 39360 - - ACCCCGGAGA CCTCTTCGCC GCCCTCGGCA CGCGCGTCCT CCTCCCGGTT CT -#CCATCACC 39420 - - ATGCAGAACC ACGACAGCAG CCCTGCCAGG GAGATGTAGA AGGCCACCAG AA -#CTTGGCCG 39480 - - CTCACTTCAC CATTCCTCGA ACCCACCAGC GAGACAGCGC CTTACGCCCT TT -#GTCGAGCG 39540 - - GGGTCAGCTC GCGCTCATCG TCCTCACCGA AGTCGAACTC GATGCTGGCG AT -#CTCGTAGC 39600 - - CGAGGATCTT GAACGACACG TTCATAGGCG GTCTCCGAAG TTGATGACGG GA -#ATGCCGGC 39660 - - CCTTTCGGCC TCTCGCATGC AGTGCCGGGT GCCGACTGAG TTGCCGAGGG GG -#AACGCCAG 39720 - - ACAGATGTCC GCACCGGCCC TGACCATCTC GATGTTGCGG AGGATGCCAG CC -#CGCTTGCC 39780 - - GTAGCGTTCC CAGTCGGCTC GGTGCAGCTC GGGGAGCACG TCCCATCCCT CC -#TGCTTCAT 39840 - - CCCCCAGGCC CAGCGGTCTG CGATGTCGTC AGCGCCGCGA GCGCCGCCGT GG -#ACGACCGT 39900 - - GAGACCGGAG AAGGACCGGT GGTACTCAGT GGCCAACGCT TCCCAGACCG TG -#GTGCGGTC 39960 - - CTTCCAGATC CGAGATCCGG TGATCAGTAC TCGCCGCATC AGATCGCCTC CC -#ACTGCAGG 40020 - - CCGTCGTGCG ACGTGACCAG CTCCGCTTCG TAGACGCCGT AGCGGGTGGC CA -#GGAACTGG 40080 - - ATCATCTGCG CCTGCTTGTA CCCGAAGGGA CATTCGTGGA CGCCGCTGAT CG -#GGTATCTG 40140 - - ACTCCGTATT TCACTTGATC CACCGCTTCG CGATTCGGTC GACGTTCTCC TC -#GGAGACGT 40200 - - TGCGGGCGAG GCCGGTGAAC TCCTGGCCGT GGACCTTGGT CTCGATCACG CG -#AGGCTTGC 40260 - - GGGGATCCGG GCTCTCCGGG TCGATCCGCT TGTGGGTCCA GACGGTCGGC TT -#CGTCTTGA 40320 - - TCAGAGCGCC CAGCACCTGC TGGCGCAGTG GGTTGGTCTT GCGGGGCATA GC -#GTTTGGAG 40380 - - TGGTCATCTG GATCCTTTCC TCGGTGGCTG TCAAGTCGGT GTGCGTAGTG AA -#GCCCCCCC 40440 - - AGGCATGCGC GCCCCGCCTG GGGAGAGTTG ATCAGCGCAG TTCGATGTCG GG -#CAGGATCG 40500 - - CCTGCGGCTT GAAGTTGACC TGGTAGAAGT CGGTCGAGAC GTTTGCGCCA TC -#GACCTGCT 40560 - - CCATGAAGTA GGAGACGTTG TCCGACAGGC CCAGGAAGTG CTTCTTGATC CC -#GTCCTTGG 40620 - - TCTTGCAGGT CACGTCGAGC TTCTTCGACG CGGTGTCCGC GTTGATTGAG CA -#CCGGCCCT 40680 - - GGATCTCGAG CAGGTACTTG TCCGTGATCC CGTTGAAGAA CACGATCCGG CG -#ATTGATCT 40740 - - CGAAGTTGTC AGCGGCCTTG CTGACGTTCT CCGATGCGAC GTCGGCGTCG GA -#GGTACACG 40800 - - CGGAGAGGCC CAGGATCGCC GATCCGGCGA TGAGTGCGGT GGCGATGATC TT -#CTTCATGT 40860 - - TCGCTACTTT CTGTTTGGTG GATGTCAAGT TAGTGACCGA AGTCGTTGAT CT -#GCATAGTG 40920 - - TCTCCGACGA ACTCCAAGGA AGCGAAGTCT TGTCCCGACG GGTCCGACTT CC -#CCCCTCGG 40980 - - TTCTTGACCG TGGAGACGTT GAGCATGTCC GGGCCGAACC CGTCCGATAC TC -#GGTGGAGA 41040 - - GTGAGGATCA TCTCAGGAAC ACGCCCGATC TGACCTTTGA TGCCCGACAA CG -#GGATCGGC 41100 - - TTGTCGCCGT CGTTGTGCGG GCCGGTGACG TGGTGGAGCC CGACGACGCA TG -#AGCCTGTC 41160 - - TCACGGCCCA TCTCGTGTAG GTAGTCCATC AGCGACTCCA GACCCGAGAA CG -#GGTCGTCT 41220 - - CCCTCGCTTG AATCGGTGCG GACGTTGGTG ATGTTGTCCA CGACGATCAA CG -#CTGGGAAG 41280 - - TCCTCGTACA GCGCGTCATA CGCGGCCAGA GCGTTCTCGA TCTCGTCCAA CG -#ACGGTGAT 41340 - - GCCTTGTAGT TGAACCGGAT CGGGATCTCG TCTAGTGAGT CAGCTACCGC GT -#CCTCGATG 41400 - - TTCTGCTCGC GAACAGCCCG CGTAGCTCGT TCGAGCGACC ATCCGCTGAG GA -#TGGACACC 41460 - - GAACGGGAGA GCTGGGTGAA CGCATCAGAG TCGGCCGAGA AGTACAACGT CG -#GCACCTTC 41520 - - GACTTGAGCG CGTAGGCGAG GACGAACGCC GACTTCCCGG TGCCGGGGCC GG -#CGCAGACC 41580 - - AGGACTAGCT GGCCTCGTCG GAGATGTGTA CCTTTCTGGT CAAGCGCGGC CC -#AGACCGGG 41640 - - GGTAGCGGAT CCCCCGCCGA CCCTCGGATG TAGAGCGATT GTCTAGGTGT GT -#ACACCTTC 41700 - - CTCCTCGTGG ATGTGATTGA CCAGGTCATA GATCTCGTCG CGAGAGACCA GC -#CGGCCCCA 41760 - - GGCGTCGATC CCCACGTGGA TCTGTCTCCG GTGGATGTGT CGGGACAGGA TC -#ATCGGCGA 41820 - - ATGCGTGTGC CCGTGGATCA GGATCTTGCC ATCGTCACGG AGCCTCCACT GG -#GTGTGTCG 41880 - - GTCCTCGCTG GTGTGGTCCC CGACGTATGG GAAGTGGCTC AGCAGAACAT CT -#GTGTGCCC 41940 - - GCCAGCGTCC CCGTACAGCG GCACCCGGAT ACGAGCTGCC GTCGACACAT GC -#TCGAACAC 42000 - - CATCCAGTAC GCACCAACCA GCTTGTGAGC ATCGCGGTTC ATCGGGTGGG GC -#CCATCGTG 42060 - - GTTGCCCAGG ATCAGCCGTT TGCGGCCTGG CCGATCCGAG ATCCACCCGA GG -#GCATGTAT 42120 - - CTGCCCCTTG GTGGAGCCAG AGGAGATGTC ACCTAGGATC CAGACCGTGT CG -#TCCTTGCC 42180 - - GACGACCGAG TCCCACGCCT TCGCCAGGGT GGCGTCGTGC TCTTCGACAT CA -#TCCGCCAG 42240 - - GTTGCGGATC TCCATCAGCC GCTTGTGTCC GATGTGTAGA TCGGACGTGA AC -#CAGGTGTT 42300 - - GCTCATGGCT TCCTTTCAGA ACGGCGGGCC GTACAGCTCG ATCACCAGCG CG -#TGCAGCTC 42360 - - CTCTGCCGCG TCGTCACGCT CGAATCCGCA GCAGGAATCG TGCCGGTCGA GG -#ATTGCGAC 42420 - - GATCTGGTCG TAGAGGCTGG GCCTCACTTC ACCTTCTTCG GATCGATCAA GG -#CGTCGTGA 42480 - - ATCGGCCGAC CGGCGCGAGC CGCGTGCGTC TCGGCGTCCA AGGCTCGCTG CA -#TCTGGTTC 42540 - - ATCAGCCGGG TGCCGCGCAG CTTGAGGATC TTCATGGTCG CCCGACCCTT GT -#ATCCAGCG 42600 - - CGGTGCATCC GTAGGACGCA GGCTGTCTCG TGCGGGGCTA TAGGTGACCT CA -#GCGACGGG 42660 - - TGGTTTGGAT CCCAGTTCGT CATGTCTTCC TCTCGGTGGC TGTCAAGTTG GT -#CACAGACC 42720 - - GAACTCTTCC TGGTACTGCG GGATGAAGTG GCCGGCCGTT CATGTTCGGC TC -#GATACCTC 42780 - - TCGCGTCACG AACTCCTGCC CGTTCCATCT CCGACCGTCC TCGAACTCGA TC -#ACGATCTC 42840 - - TCGTCCGGGA TGACGCACGG CCTCCGCTTG GGCAAACCTG CGTGCAGCCT CT -#GGGGTCGG 42900 - - GAACGGAAAC TTCTGCGAGG CGTACAGCTC CTGGTGCCAC TTCGGCTTGT CA -#GGAATCGG 42960 - - CCCCATTTCC ACGTACGTGT AACCCGCGTC GGGGTCGAGT TCGAGCGTTT TC -#TTGTATTC 43020 - - CTTCGTGCCT GCCTTAGAGG GAAGGTGAGT ATCGGTGGCT GTCAAGGTGA CC -#TCACTTAA 43080 - - AAACAGGGCA GCTGTAATTC ACATCACAGA AGCCGCATTT GTCAGGTTCA GG -#CAGAGGCT 43140 - - CGAAGTCACC AGCCTGGATC CGAGCCTCGA CCTCATGGAA CCTCTCGGTG AT -#CCGCTCCC 43200 - - GCGTCCAATC GGTCAGGTCG TAGGGCGCAG TGGGCTTCGC CTTGATGCCC TT -#CTTCCCCG 43260 - - CCATGAAGTA GTCGCCCGTC TTCGGAGCCT CCACGTCATA GGTCATCGCG AC -#CGCGAGCG 43320 - - CGTACACGCC GAGCTGGAAG TCGTCACCCG GCGAGTTGCC GGTCTTGTAG TC -#CCGGACTC 43380 - - GAAGCTCACC GTTGACCACG ACGACCGCGT CGATGAACCC TCGGACGCGG AT -#GCCGTCCA 43440 - - GCTCGATGTT GAACGGAAGC TCGATGGCCG GCTTGGGCTG TTCACACTCC TT -#GCAGTTGG 43500 - - TGTCTTTCCA CGCCTCCGTA GAGCAGATCC CTCGCCCAGG GGTAGTCCAG AT -#CTGCTGGC 43560 - - CCTTGTCCTT CCGCCACGCG ATGAACTTCT CTACCTGCTC CAGTCCAAGG TG -#GAACCGGC 43620 - - GCTCGATGTC ACGCTCACCG TTGTACGGCC CGGACCAAAA CCACCACTCG AA -#GTTCGGGG 43680 - - TTTCGTCGCA CAGTGCTCCG ATGTCCTTGG CGTACTCCTC GCGGAAGATC TC -#TTGTGCCC 43740 - - GTTCGAGGCT CATCTCGCGG CCCTCGGCCA GAGCCTTCTC GTAGACCTCA GC -#GACGGTGT 43800 - - GAAACGCGGT GCCCTGCGGC AACCACGCCG CAGGACGAGC CCATACCTTG TC -#GATGCGAG 43860 - - CCAGCTTGTA CGCCTGCGGG CAACGTGTGT ATTGGTTCAA CTGGCTGACG CT -#TCGCAGCG 43920 - - GCAGCAATGT CTTGGTGTCT GTCACGCAGC GGCCATCCTT CCCTTGCCTA TC -#GTCTCGTT 43980 - - CAGCGCCCCG TCGACAGCGA CACTGAGCAG TTTTGCGACC TCCGACATGT CA -#ATCGGATC 44040 - - CTTGGGGAAT TGGTCAGCCT GAGTCATCCT GAGCACCATC CACTCGGTGC CC -#TTGTCGCA 44100 - - GTGGATCATG GTCGGATCCT TAATTAAGAT CCTTTAGTGA GGGTTAATTG CG -#GCCGCGAA 44160 - - TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TC -#ATGATAAT 44220 - - AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CC -#CCTATTTG 44280 - - TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CC -#TGATAAAT 44340 - - GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TC -#GCCCTTAT 44400 - - TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TG -#GTGAAAGT 44460 - - AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG AT -#CTCAACAG 44520 - - CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GC -#ACTTTTAA 44580 - - AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AA -#CTCGGTCG 44640 - - CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AA -#AAGCATCT 44700 - - TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GT -#GATAACAC 44760 - - TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CT -#TTTTTGCA 44820 - - CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA AT -#GAAGCCAT 44880 - - ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TG -#CGCAAACT 44940 - - ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GG -#ATGGAGGC 45000 - - GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TT -#ATTGCTGA 45060 - - TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GG -#CCAGATGG 45120 - - TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TG -#GATGAACG 45180 - - AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TG -#TCAGACCA 45240 - - AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AA -#AGGATCTA 45300 - - GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TT -#TCGTTCCA 45360 - - CTGAGCGTCA GACCCCTTAA TAAGATGATC TTCTTGAGAT CGTTTTGGTC TG -#CGCGTAAT 45420 - - CTCTTGCTCT GAAAACGAAA AAACCGCCTT GCAGGGCGGT TTTTCGAAGG TT -#CTCTGAGC 45480 - - TACCAACTCT TTGAACCGAG GTAACTGGCT TGGAGGAGCG CAGTCACCAA AA -#CTTGTCCT 45540 - - TTCAGTTTAG CCTTAACCGG CGCATGACTT CAAGACTAAC TCCTCTAAAT CA -#ATTACCAG 45600 - - TGGCTGCTGC CAGTGGTGCT TTTGCATGTC TTTCCGGGTT GGACTCAAGA CG -#ATAGTTAC 45660 - - CGGATAAGGC GCAGCGGTCG GACTGAACGG GGGGTTCGTG CATACAGTCC AG -#CTTGGAGC 45720 - - GAACTGCCTA CCCGGAACTG AGTGTCAGGC GTGGAATGAG ACAAACGCGG CC -#ATAACAGC 45780 - - GGAATGACAC CGGTAAACCG AAAGGCAGGA ACAGGAGAGC GCACGAGGGA GC -#CGCCAGGG 45840 - - GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCACTGATT TG -#AGCGTCAG 45900 - - ATTTCGTGAT GCTTGTCAGG GGGGCGGAGC CTATGGAAAA ACGGCTTTGC CG -#CGGCCCTC 45960 - - TCACTTCCCT GTTAAGTATC TTCCTGGCAT CTTCCAGGAA ATCTCCGCCC CG -#TTCGTAAG 46020 - - CCATTTCCGC TCGCCGCAGT CGAACGACCG AGCGTAGCGA GTCAGTGAGC GA -#GGAAGCGG 46080 - - AATATATCCT GTATCACATA TTCTGCTGAC GCACCGGTGC AGCCTTTTTT CT -#CCTGCCAC 46140 - - ATGAAGCACT TCACTGACAC CCTCATCAGT GCCAACATAG TAAGCCAGTA TA -#CACTCCGC 46200 - - TAGCGCTGAG GTCTGCCTCG TGAAGAAGGT GTTGCTGACT CATACCAGGC CT -#GAATCGCC 46260 - - CCATCATCCA GCCAGAAAGT GAGGGAGCCA CGGTTGATGA GAGCTTTGTT GT -#AGGTGGAC 46320 - - CAGTTGGTGA TTTTGAACTT TTGCTTTGCC ACGGAACGGT CTGCGTTGTC GG -#GAAGATGC 46380 - - GTGATCTGAT CCTTCAACTC AGCAAAAGTT CGATTTATTC AACAAAGCCA CC -#GAACGCCA 46440 - - GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TT -#CTCGCCGA 46500 - - AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGGAAG AT -#TCCGAATA 46560 - - CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GGGGTCCTCG CC -#GAAAATGA 46620 - - CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GT -#CATAAGTG 46680 - - CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AA -#GGCTCTCA 46740 - - AGGGCATCGG TCGAGGAACT TTCGGCGGCT TTGCTGTGCG ACAGGCTCAC GT -#CTAAAAGG 46800 - - AAATAAATCA TGGGTCATAA AAATTATCAC GTTGTCGGCG CGGCGACGGA TG -#TTCTGTAT 46860 - - GCGCTGTTTT CCGTTGGCCG TTGCTGTCTG GTGATCTGCC TTCTAAATCT GC -#ACAGCCGA 46920 - - ATTGCGCGAG CTTGGTTTTG CTGAAACCGA CACACAGCAA CTGAATACCA GA -#AAGAAAAT 46980 - - CACTTTGCCT TTCTGACATC AGAAGGGCAG AAATTTGCCG TTGAACACCT GG -#TCAATACG 47040 - - CGTTTTGGTG AGCAGCAATA TTGCGCTTCG ATGAGCCTTG GCGTTGAGAT TG -#ATACCTCT 47100 - - GCTGCACAAA AGGCAATCGA CCGAGCTGGA CCAGCGCATT CGTGACACCG TC -#TCCTTCGA 47160 - - ACTTATTCGC AATGGAGTGT CATTCATCAA GGACNGCCTG ATCGCAAATG GT -#GCTATCCA 47220 - - CGCAGCGGCA ATCGAAAACC CTCAGCCGGT GACCAATATC TACAACATCA GC -#CTTGGTAT 47280 - - CCTGCGTGAT GAGCCAGCGC AGAACAAGGT AACCGTCAGT GCCGATAAGT TC -#AAAGTTAA 47340 - - ACCTGGTGTT GATACCAACA TTGAAACGTT GATCGAAAAC GCGCTGAAAA AC -#GCTGCTGA 47400 - - ATGTGCGGCG CTGGATGTCA CAAAGCAAAT GGCAGCAGAC AAGAAAGCGA TG -#GATGAACT 47460 - - GGCTTCCTAT GTCCGCACGG CCATCATGAT GGAATGTTTC CCCGGTGGTG TT -#ATCTGGCA 47520 - - GCAGTGCCGT CGATAGTATG CAATTGATAA TTATTATCAT TTGCGGGTCC TT -#TCCGGCGA 47580 - - TCCGCCTTGT TACGGGGCGG CGACCTCGCG GGTTTTCGCT ATTTATGAAA AT -#TTTCCGGT 47640 - - TTAAGGCGTT TCCGTTCTTC TTCGTCATAA CTTAATGTTT TTATTTAAAA TA -#CCCTCTGA 47700 - - AAAGAAAGGA AACGACAGGT GCTGAAAGCG AGCTTTTTGG CCTCTGTCGT TT -#CCTTTCTC 47760 - - TGTTTTTGTC CGTGGAATGA ACAATGGAAG TCAACAAAAA GCAGAGCTTA TC -#GATGATAA 47820 - - GCGGTCAAAC ATGAGAATTC GCGGCCGCAT AATACGACTC ACTATAGGGA TC -#TTAATTAA 47880 - - GGCGCCTGAT CAGGATCAGG TCGATGGCTT TGTTGTTCTC CGGGCAGCGC AC -#CGCCGTCG 47940 - - GAAACTCGGC CTTGCCTTTG GCGAACGTGG TGTCGACGTA GGCGATGTTG AT -#GCCCTTGT 48000 - - CTTCCAAGAA GCGCGCCACG TCGATGTTGT CCGGGTCTGC GCTGAAGTAC AG -#CGCCAGGT 48060 - - TGTCGAGCCT CTGCGAGTGC AGGTAGACAG CCGCCGTCTG AACCCTTGTG TA -#GGCCCAGA 48120 - - ACTGGACATC CGGGTTGTCG CGGATGACTC GACCCCAAGC GGCCACATAG GT -#GGGGCTGA 48180 - - AGAAGTCTCC ATCCCAGTGG ATGCGGAACA GCTTCGGAGC CTTGCGACGG TC -#GCAATCCT 48240 - - TGACGAACTC GGCGACCATC TCGGACAGCA GCGTCACGGT GTCTGTCAAG TC -#AGCGTCAC 48300 - - GCAACAGTTC CCAGTTGTGC AGCAGGACCG AGCTGACAGC CTTGCGAACT TT -#CTCCAGCT 48360 - - TGCCGGCGTA GCACACCTTG GCACAGAAGG CCGTCGCGTC CGGGCAGGAG AA -#GCCTTGAC 48420 - - CGGAGGGCAG GCCGATGCTG TTGGCGATAC CTACGGTGGC GTTGCCGCCC TT -#GGTGACGT 48480 - - GGACGTAGTT GGTGACCTTG CGGTCGTTCG AACGCTTCAG CTTGGCCATA CC -#TAGCCTTC 48540 - - CTTCGGTGGC TGTCAAGTTG TTGGATACAA AGCGCCCCGA GAGGGAGTCG AA -#CCCTCACA 48600 - - CCGCGAACCG TCGCGGGGCC ACCGTGCCTA GTCGATAGAG GTCACTCGAC TC -#TCGTGGAC 48660 - - GTAGACCACG GTGTTGCCTA CGTTCACCGC GTAGTACAGG CCATCGGCAC CT -#CGTAGCTT 48720 - - GTGCCGAACC GTGCCCGACG TGGCCGTCAT GTCTTCGCCC CAGTCGGCGT TA -#GGTGCCCA 48780 - - GGTGACTCGC ATGGTGATCC CTTCAGTAGT CGGTGGCTGT CAAGTCAGCG GA -#TACGGACG 48840 - - TACCCGTTGC CTCGAGCGAC GTAGATCTTG CCGTCGATGT AAACGCGCTG CT -#GCTGGTTC 48900 - - ATAATCCTAT TCCTTTCGGT GGCTGTCAAG TCTCAGGCCC AGCGACGAGT CG -#TCGGCCGG 48960 - - GGGCGGCGCA CCTTGGGCGC GTTGGCTCGC GGTGCCTTAC GGATGGCGGT GC -#CTACCGTG 49020 - - ATCTCTTCCA ACTGGCGTTC AGCCAGGCCG ACAGGCCGGG CGTCACCGGG CA -#GTTCGATC 49080 - - TTGTAATCGA AGTCAGTCCA CCCCTTCAGA CCCTTCTCCA GCTCGCGATC CA -#ACAGACGC 49140 - - GGAGCCGACA GCTCAGGCGC AACAAACGGT GTCTTGACGC TCTCGCGGGC AG -#TAACCCGA 49200 - - ACCTCACGGT GCTCAGCGAA GACTGGCATA GTTCACCCCT TTGGTGGATG TC -#AAGCCTGA 49260 - - GCACCAAAGC TCAGGCGTAG TGGGTAGTCG GGAATCGAAC CCGATAGCTT CA -#TAGCCACG 49320 - - TTCTACGGCT CAGCCATAGC TCAGCGATCA TTCCATCGCG CCAAGAGCTA CC -#CTCCCGAA 49380 - - TGCCGAACCA AAGCTCAGCA TTCGTAAGTG TGTATTCTCC CCGTGGCTCA GA -#CAGTATCT 49440 - - ATCAGAACCT AACCACAGGT CTACATTTAG TTATCCGCAG TGCTCGCACT TT -#AACGGCAT 49500 - - CGAGCTTCCG CCGACCCTCA GTCCTCTGGC AGCGAACTAA AGGTTTGAGT CG -#GGCTGCGG 49560 - - CCCTTCTCGG TCTTGCGTGA TTCTCACTCT ACCGGATGTT TCGGTGGCTG TC -#AAGCGGGC 49620 - - CGTTTTGGTG TTGCAACGAT GCCCTCGTTT AGCGCCGCTG GCGTAATGCG CT -#ACCCGCCT 49680 - - GATCTCACCG GTCCAAGTTG GTGATGCTTG CAGCTTACCC GATAACCGGG TG -#GCTGTCAA 49740 - - ACCGGAGAAT CTTGCCGCCG GATTTTCACC GGCACCGGCA CGATCCTCTC GG -#ATCCGCCT 49800 - - ACCGCCTTGC TGCTGCGGTG ACACAAGAAT GCACTACTGG CCGGGTGGCT GT -#CAAGCCCT 49860 - - AATCGCAAAT TGGTGCCCTA GCTGCAGATA TGGCGCGTTC TCGGTGGCTG TA -#AAGGGCAC 49920 - - TACGTGCCGC TATCCGCTGG TCACGCTGGA CAGTCCCGGC AGCCCGTGCC GC -#GCATAGGC 49980 - - TGCTCACTAC GTGCCCGGTA TCGGCGTTGT CGTGCCGCTG TCGTGGTCGT CG -#CCCCGTCG 50040 - - CTGTCGCTGG TCTCGGTGGC ATCGCTTGAC AGTCGCCCCG CTATCCCCCG TT -#GCCGCTGG 50100 - - TCAGACGCTA ATCCGCTTAT TTCGCATAGG CTGCTCACTA TCGCATCGGT AT -#GCGTATGC 50160 - - GCTGGTCACA TATGCGTGTG GTGGTGGTGT GGTGTGCGTG TGTTTGCGCT GG -#TCAGCCGT 50220 - - GTGCGTACCG TATCCGCACA CTGTGCTTGT GCGTTTGCTG TGTGTCGAGG CC -#GGCTCTCG 50280 - - CATCGTCGCA TGTCAGCGCG GGTATGGGCG TGTATCGCAC GCTTTGCTAG CC -#GCGTGCCG 50340 - - C - # - # - # 50341__________________________________________________________________________

	Number	Date	Country
Parent	057531	Apr 1993
Parent	833431	Feb 1992

L5 shuttle phasmids

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CPC

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Abstract

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CROSS REFERENCE TO RELATED APPLICATIONS

STATEMENT OF GOVERNMENT INTEREST

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