Claims
- 1. A composition comprising an enzyme exhibiting laccase activity and an enzyme enhancing agent selected from 2-thiouracil, sodium dimethyldithio carbonate hydrate, N-benzylidene-benzylamine, melamine, anthracene, dicyandiamide, sulfanilic acid, sulfanilamide,urea, salicylic acid, 3,4,5-trihydroxy-benzoic acid, ferric chloride, potassium ferricyanide, ascorbic acid, Zincon (o-[2-[alpha(2-hydroxy-5-sulfophenylazo)benzylidene]hydrazino]benzoic acid), diisopropanolamine, adenosine triphosphate, guanidine, cyanuric acid, Thiazol Yellow G, nicotinic acid, Metanil Yellow, hardwood black liquor, softwood black liquor, methanesulfonic acid, metanilic acid, sulfamide, 3-pyridine sulfonic acid, benzofuroxan, t-butyl hydroperoxide, pyruvic acid, imidazole, N-acetylcytosine, and phenol.
- 2. The composition of claim 1 wherein said enzyme exhibiting laccase activity is selected from a laccase enzyme of enzyme classification EC 1.10.3.2, a catechol oxidase enzyme of enzyme classification EC 1.10.3.1, a monophenol monooxygenase enzyme of enzyme classification EC 1.14.99.1, a bilirubin oxidase enzyme of enzyme classification EC 1.3.3.5, and an ascorbate oxidase enzyme of enzyme classification EC 1.10.3.3.
- 3. The composition of claim 1 that further comprises a hydrolase.
- 4. The composition of claim 3 wherein said hydrolase is a xylanase.
- 5. A process for oxidizing a substrate, comprising treating the substrate with a composition comprising an enzyme exhibiting laccase activity and an enzyme enhancing agent selected from 2-thiouracil, sodium dimethyldithio carbonate hydrate, N-benzylidene-benzylamine, melamine, anthracene, dicyandiamide, sulfanilic acid, sulfanilamide,urea, salicylic acid, 3,4,5-trihydroxy-benzoic acid, ferric chloride, potassium ferricyanide, ascorbic acid, Zincon (o-[2-[alpha(2-hydroxy-5-sulfophenylazo)benzylidene]hydrazino]benzoic acid), diisopropanolamine, adenosine triphosphate, guanidine, cyanuric acid, Thiazol Yellow G, nicotinic acid, Metanil Yellow, hardwood black liquor, softwood black liquor, methanesulfonic acid, metanilic acid, sulfamide, 3-pyridine sulfonic acid, benzofuroxan, t-butyl hydroperoxide, pyruvic acid, imidazole, N-acetylcytosine, and phenol.
- 6. The process of claim 5 wherein said enzyme exhibiting laccase activity is selected from a laccase enzyme of enzyme classification EC 1.10.3.2, a catechol oxidase enzyme of enzyme classification EC 1.10.3.1, a monophenol monooxygenase enzyme of enzyme classification EC 1.14.99.1, a bilirubin oxidase enzyme of enzyme classification EC 1.3.3.5, and an ascorbate oxidase enzyme of enzyme classification EC 1.10.3.3.
- 7. The process of claim 5 wherein said treating further includes a hydrolase.
- 8. The process of claim 7 wherein said hydrolase is a xylanase.
- 9. The process of claim 5 that further comprises adding an oxidizing agent.
- 10. The process of claim 9 wherein said oxidizing agent is at least one of air, oxygen, and hydrogen peroxide.
- 11. A process for bleaching a lignin-containing material, comprising treating the material with an enzyme exhibiting laccase activity and an enzyme enhancing agent selected from 2-thiouracil, sodium dimethyldithio carbonate hydrate, N-benzylidene-benzylamine, melamine, anthracene, dicyandiamide, sulfanilic acid, sulfanilamide,urea, salicylic acid, 3,4,5-trihydroxy-benzoic acid, ferric chloride, potassium ferricyanide, ascorbic acid, Zincon (o-[2-[alpha(2-hydroxy-5-sulfophenylazo)benzylidene]hydrazino]benzoic acid), diisopropanolamine, adenosine triphosphate, guanidine, cyanuric acid, Thiazol Yellow G, nicotinic acid, Metanil Yellow, hardwood black liquor, softwood black liquor, methanesulfonic acid, metanilic acid, sulfamide, 3-pyridine sulfonic acid, benzofuroxan, t-butyl hydroperoxide, pyruvic acid, imidazole, N-acetylcytosine, and phenol.
- 12. The process of claim 11 wherein said enzyme exhibiting laccase activity is selected from a laccase enzyme of enzyme classification EC 1.10.3.2, a catechol oxidase enzyme of enzyme classification EC 1.10.3.1, a monophenol monooxygenase enzyme of enzyme classification EC 1.14.99.1, a bilirubin oxidase enzyme of enzyme classification EC 1.3.3.5, and an ascorbate oxidase enzyme of enzyme classification EC 1.10.3.3.
- 13. The process of claim 11 wherein said treating further includes a hydrolase.
- 14. The process of claim 13 wherein said hydrolase is a xylanase.
- 15. The process of claim 11 that further comprises adding an oxidizing agent.
- 16. The process of claim 15 wherein said oxidizing agent is at least one of air, oxygen, and hydrogen peroxide.
- 17. The process of claim 11 wherein said material is a wood pulp.
- 18. The process of claim 17 wherein said wood pulp is a raw material used to form a polysaccharide or a cellulose derivative.
- 19. A process for enhancing the activity of an enzyme exhibiting laccase activity, comprising adding an enzyme enhancing agent to said enzyme, wherein said enzyme enhancing agent is selected from 2-thiouracil, sodium dimethyldithio carbonate hydrate, N-benzylidene-benzylamine, melamine, anthracene, dicyandiamide, sulfanilic acid, sulfanilamide,urea, salicylic acid, 3,4,5-trihydroxy-benzoic acid, ferric chloride, potassium ferricyanide, ascorbic acid, Zincon (o-[2-[alpha(2-hydroxy-5-sulfophenylazo)benzylidene]hydrazino]benzoic acid), diisopropanolamine, adenosine triphosphate, guanidine, cyanuric acid, Thiazol Yellow G, nicotinic acid, Metanil Yellow, hardwood black liquor, softwood black liquor, methanesulfonic acid, metanilic acid, sulfamide, 3-pyridine sulfonic acid, benzofuroxan, t-butyl hydroperoxide, pyruvic acid, imidazole, N-acetylcytosine, and phenol.
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application claims the benefit of U.S. Provisional Application No. 60/318,290, filed on Sep. 10, 2001.
Provisional Applications (1)
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Number |
Date |
Country |
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60318290 |
Sep 2001 |
US |