Lactobacillus reuteri and use thereof

Abstract

Provided is a strain CCFM8631 of Lactobacillus reuteri and use thereof. The strain CCFM8631 of Lactobacillus reuteri can significantly increase neurotransmitter 5-hydroxytryptamine level in peripheral blood, recover the hormone levels, for example testosterone and so on in peripheral blood of rat, normalize abnormal abundances of Blautia genus and Turicibacter genus, Oscillospira genus and Bifidobacterium genus in intestinal flora of rat affected by high-fat high-starch diet, show good tolerance to simulated gastrointestinal fluid and quickly colonize in intestinal, significantly improve pathological damages of tissues such as liver, duodenum and so on, and increase triglyceride and total cholesterol levels in serum and oral glucose tolerance of rat with metabolic syndrome caused by high-fat high-starch diet. The strain CCFM8631 of Lactobacillus reuteri can be used for preventing, relieving or treating metabolic disorder, such as metabolic syndrome, irritable bowel syndrome and mental diseases related to metabolic syndrome such as anxiety, depression and so on.

Description

CROSS REFERENCE TO RELATED APPLICATIONS

This application claims the priority of Chinese Patent Application No. 201710963119.2, filed on Oct. 17, 2017, and the disclosures of which are hereby incorporated by reference.

FIELD

The present disclosure relates to the field of microbe technology, specifically to a Lactobacillus reuteri and use thereof, especially to a Lactobacillus reuteri that is capable of modulating intestinal flora, modulating brain-gut axis and significantly alleviating metabolic syndrome, and use thereof.

BACKGROUND

Recent years, with the developing economy, life-styles of people in many countries have changed obviously. With the amount of physical activity has decreased, obesity rate has increased significantly, and prevalence rates of diabetes and metabolic syndrome have increased by a large margin. An epidemiology survey shows that 20% to 30% of the adults across the globe are suffering from metabolic syndrome. In 2013, a multicenter, multistage stratified, large-scale sampling survey carried out by Chinese Diabetes Society of Chinese Medical Association showed that among people over 20 years old in large and medium-sized cities, towns and countryside of China, prevalence rates of metabolic syndrome in men and women were 16.7% and 11.7%, respectively, and the total prevalence rate was 13.7%. Furthermore, the rate was continuously increasing. Analyses show that age, blood pressure, diabetes family history, obesity, hyperlipidemia, male, low income and little exercise are main related risk factors for metabolic syndrome. The survey also found that the rates of the overweight and the obesity among people have increased by a large margin, and prevalence rate of metabolic syndrome of male is significant higher than that of female at the same age among middle-aged crowd.

Metabolic syndrome is a clinical syndrome, which has simultaneous symptoms of central obesity, fasting blood glucose rising, high blood pressure, decrease of high-density lipoprotein cholesterol and increase of triacylglycerol, in which numerous hazardous factors basing on the abnormal pathological changes of carbohydrate metabolism, lipid metabolism and protein metabolism aggregates, and which promotes development of diabetes (type II) and cardiovascular diseases such as atherosclerosis and so on. Due to metabolic syndrome is a pathological condition in which numerous metabolic abnormalities aggregates, its occurrence is relates to insulin resistance, becoming a hot spot in the research field of cardiovascular diseases and diabetes, and raising many controversies at home and abroad. In addition, metabolic syndrome accompanies with disorder of intestinal microecosystem. Disorder of intestinal microecosystem may further lead to disorder of intestinal functions, nerve center functions and peripheral nerve functions, for example, intestinal inflammation, inflammatory bowel disease (IBD) and irritable bowel syndrome (IBS), abnormality of neurotransmitter 5-hydroxytryptamine level and level of some hormone and so on. Many researches have shown that abundances of intestinal microbes of some genus have intimate connection with intestinal diseases, for example, the abundance of Blautia genus will rise in intestinal flora of IBS patient, and the abundance of Oscillospira genus is closely related to the body mass index. At the same time, researches also show that mental diseases such as depression, anxiety and so on have intimate connection with metabolic syndrome, intestinal flora disorder, and low 5-hydroxytryptamine level in human body. Improving 5-hydroxytryptamine level in peripheral blood helps increasing neurotransmitter level of central nervous system, so that relieving symptoms of anxiety, depression and so on.

At present, all the drug treatments of metabolic syndrome aim to decrease all kinds of risk factors, and these drugs include: anti-obesity drugs for weight loss; dimethylbiguanide and thiazolidinediones for reducing insulin resistance; sulfonylurea and rosiglitazone for controlling blood glucose; fibrate and statin for improving disorder of lipid metabolism; captopril, amlodipine and so on for controlling the blood pressure; drugs for treating intestinal diseases such as IBD, IBS and so on, including glucocorticoid, immunosuppressant, psychotropic drugs and so on; drugs for mental diseases such as anxiety, depression and so on, including selective serotonin reuptake inhibitors such as paroxetine, noradrenaline, and specific 5-hydroxytryptamine antidepressant drugs such as mirtazapine and so on. All of the medicines above have certain therapeutic effects, but as the conditions getting worse, the amounts of medicine used increase, the interactions between the medicines as well as the toxic and side effects of medicines become significant, leading to adverse reaction of digestive tract and showing liver and renal toxicity in some degree. In consideration of problems of the medicines, early intervention in metabolic syndrome, intestinal disease and mental disease can effectively decrease onset risk of cardiovascular and cerebrovascular diseases, diabetes, inflammatory bowel diseases, depression and so on.

Probiotics are edible microbes that are beneficial to human health, which have potential functions of alleviating abnormal metabolism of blood glucose and blood lipid, and modulating intestinal flora proportion and brain-gut axis. Thus, there is important social and economy value to research and develop probiotic products that can effectively intervene the occurrence and development of metabolic syndrome, intestinal diseases and mental diseases.

At present, there is no patent about using probiotics to increase 5-hydroxytryptamine level so as to regulate brain-gut axis and relieve anxiety and depression. There is also no patent about modulating the abnormal abundance of Oscillospira genus to relieve metabolic diseases. Although there is related patent application (CN107083339A) that discloses adding Blautia bacteria to protect piglets from diarrhea, there is no patent about modulating the abnormal abundance of Blautia genus bacteria in intestinal tract so as to alleviate intestinal disease by the uptake of edible microbes (list of bacterium that can be permitted to be used in food, infant food, health products by National Health Commission of the People's Republic of China, 2014). In addition, there are some patents or patent application relating to compositions and preparation method thereof for preventing and curing metabolic syndrome. For example, CN104906263A discloses a composition consisting of tea polyphenol, procyanidin and POTENTILLAE DISCOLORIS HERBA extract, which is used to treat metabolic syndrome. CN105796674A discloses a traditional Chinese medicine composition comprising PLANTAGINIS SEMEN, COPTIDIS RHIZOMA and so on, which is capable of preventing and curing metabolic syndrome. In addition, a few patents relate to probiotics-containing compositions that are used to improve metabolic syndrome, and the methods for preparing the same. For example, CN105816623A discloses a probiotic-fermented traditional Chinese medicine composition being used to cure and improve metabolic syndrome, which is made from traditional Chinese medicine such as PANACIS QUINQUEFOLII RADIX, DIOSCOREAE RHIZOMA, MOUTAN CORTEX, PORIA by extracting and fermenting the extract with probiotics. All the above patents and patent applications are using traditional Chinese medicine components or a mixture of bacteria and traditional Chinese medicine to alleviate metabolic syndrome, in which the bacteria and the functions of the bacteria are not clear. CN105567586A discloses a Lactobacillus plantarum NCU116 with antidiabetic function, which achieves antidiabetic effects through modulating body blood glucose, blood lipid, hormone level and body metabolism. The Lactobacillus plantarum is screened and selected from bacteria in kimchi instead of human sources. No evidence shows that Lactobacillus plantarum can colonize in human intestinal tract and take effects. So far, there is no a human-sourced individual probiotic (such as Lactobacillus reuteri) that can colonize in human intestinal tract to relieve the metabolic syndrome, or to alleviate symptoms such as hyperglycemia, hyperlipidemia, intestinal flora imbalance, intestinal inflammation and so on, and related mental diseases.

SUMMARY

In view of above, an object of the present disclosure is to solve the problems in the prior art by providing a probiotics. The probiotics can colonize in intestinal tract of human body, improve 5-hydroxytryptamine level in peripheral blood, regulate brain-gut axis and recovering testosterone level in serum back to normal level, normalize abnormal abundances of Blautia genus, Turicibacter genus, Oscillospira genus and Bifidobacterium genus in intestinal flora, improve metabolic syndrome, relieve hyperglycemia, hyperlipidemia and inflammation of liver and duodenum, liver fibrosis and other symptoms.

The present disclosure provides a strain CCFM8631 of Lactobacillus reuteri, which is deposited at China General Microbiological Culture Collection Center (CGMCC, Address: Beijing Institute of Microbiology, Chinese Academy of Sciences, No. 1, Beichen West Road, Chaoyang District, Beijing, China) on Jul. 7, 2017, with an accession number CGMCC 14394.

In one embodiment, the present disclosure studies effect of strain CCFM8631 of Lactobacillus reuteri on intestinal flora imbalance caused by high-carbohydrate and high-fat diet. The results show that the uptake of strain CCFM8631 of Lactobacillus reuteri significantly recovers relative abundances of Bifidobacterium genus and Turicibacter genus in disordered intestinal microbes of rat feces, and also regulates the abundances of Oscillospira genus and Blautia genus in rat feces back to normal level. The intervention effect is obviously better than that of Lactobacillus rhamnosus LGG.

In one embodiment, the present disclosure studies protection effects of strain CCFM8631 of Lactobacillus reuteri on tissue damages of liver, duodenum and so on in rat with metabolic syndrome. The results show that administration of strain CCFM8631 of Lactobacillus reuteri by intragastric gavage significantly improves symptoms caused by high-fat high-starch diet, such as hepatocyte microvesicular steatosis, interstitial inflammatory cell infiltration, early fibrosis of liver tissue, duodenum villi broadening, interstitial edema, increasing of inflammatory cells and so on in rats, and the intervention effect is obviously better than that of Lactobacillus rhamnosus LGG.

In one embodiment, the present disclosure studies effect of strain CCFM8631 of Lactobacillus reuteri on (fasting) blood glucose level of rat with metabolic syndrome. The results show that administration of strain CCFM8631 of Lactobacillus reuteri by intragastric gavage obviously decreases the fasting blood glucose level of the model rat close to the blank control group. The ability of strain CCFM8631 of Lactobacillus reuteri on decreasing fasting blood glucose level of rat is better than that of rosiglitazone and Lactobacillus rhamnosus LGG by intragastric gavage administration.

In one embodiment, the present disclosure studies effect of strain CCFM8631 of Lactobacillus reuteri on oral glucose tolerance of rat with metabolic syndrome. The results show that strain CCFM8631 of Lactobacillus reuteri significantly improves oral glucose tolerance and the effect is better than that of Lactobacillus rhamnosus LGG, indicating that strain CCFM8631 of Lactobacillus reuteri can further decrease glucose level by improving glucose tolerance.

In one embodiment, the present disclosure studies effects of strain CCFM8631 of Lactobacillus reuteri on total cholesterol (TC) and triglyceride (TG) in serum of rat with metabolic syndrome, respectively. The results show that administration of strain CCFM8631 of Lactobacillus reuteri by intragastric gavage decreases levels of total cholesterol and triglyceride in serum.

Further, in one embodiment, the present disclosure studies effects of strain CCFM8631 of Lactobacillus reuteri on 5-HT and testosterone level in serum of rat with metabolic syndrome, respectively. The results show that administration of strain CCFM8631 of Lactobacillus reuteri by intragastric gavage improves 5-hydroxytryptamine (5-HT) level in rat serum, and reduces testosterone in serum back to normal level.

Therefore, the present disclosure provides use of strain CCFM8631 of Lactobacillus reuteri in preparing products that can improve metabolic syndrome, regulate intestinal flora or regulate brain-gut axis.

Therein, the improving metabolic syndrome is to relieve symptoms of hyperglycemia and hyperlipidemia, inflammation of liver and duodenum, and liver tissue fibrosis; the modulating intestinal flora is to normalize abnormal abundances of Blautia genus, Turicibacter genus, Oscillospira genus and Bifidobacterium genus in intestinal flora; and the modulating brain-gut axis as well as relieving anxiety and depression is to increase 5-hydroxytryptamine level in peripheral blood.

The product of the present disclosure includes but is not limited to health food or pharmaceutical preparation.

Therein, the health food includes but is not limited to microbial agent or fermented food.

Further, the present disclosure provides a microbial agent comprising the strain CCFM8631 of Lactobacillus reuteri.

Preferably, the viable count of the strain CCFM8631 of Lactobacillus reuteri in the microbial agent is more than 10⁶ CFU/g

The microbial agent of the present disclosure can be prepared by routine methods.

In some embodiments, the method for preparing the microbial agent is:

inoculating the strain CCFM8631 of Lactobacillus reuteri to a modified MRS medium at an inoculum size of 2 to 4 wt %, culturing for 18 to 20 h at 37° C. under anaerobic conditions, collecting bacteria, resuspending the bacteria with a protectant to reach a bacterial density of 10¹⁰ CFU/mL, culturing the suspension at 37° C. for 50 to 70 minutes under anaerobic conditions, and drying the resulting culture.

Therein, the modified MRS medium (MRS) in the present disclosure is prepared by the specific method as follows: dissolving 10 g of tryptone, 10 g of beef extract, 5 g of yeast powder, 20 g of glucose, 5 g of sodium acetate, 2 g of ammonium citrate dibasic, 2 g of dipotassium hydrogen phosphate, 0.5 g of magnesium sulfate heptahydrate, 1 mL of Tween-80, and 0.25 g of manganese sulfate monohydrate in water, diluting the mixture to 1000 mL with water, modulating the pH to 6.5, and autoclaving at 119-123° C. for 15 to 25 minutes.

The protectant in the method of the present disclosure is an aqueous solution that contains 100 g/L to 150 g/L of nonfat milk powder, 100 g/L to 150 g/L of maltodextrin and 140 g/L to 160 g/L of trehalose. That is, the protectant consists of nonfat milk powder, maltodextrin, trehalose and water, wherein the concentration of nonfat milk powder is from 100 g/L to 150 g/, the concentration of maltodextrin is from 100 g/L to 150 g/L, and the concentration of trehalose is from 140 g/L to 160 g/L.

Preferably, in the method of the present disclosure, bacteria collected after culturing in the modified MRS medium are subjected to washing with phosphate buffer solution for 2 to 4 times, and pH of the phosphate buffer solution is from 6.8 to 7.2.

The drying of the method in the present disclosure can be any of the drying procedures for bacteria solution, for example vacuum freeze-drying. In some embodiments, the drying of the present disclosure is vacuum freeze-drying after pre-freezing the bacteria at −15 to −20° C. for 8 to 14 h.

The present disclosure also provides a fermented food which is produced by using the strain CCFM8631 of Lactobacillus reuteri as a starter culture.

The fermented food is fermented dairy products, fermented bean products or fermented fruit and vegetable products.

The fermented dairy products include but are not limited to yogurt, sour cream and cheese. The fermented bean products include but are not limited to soymilk, fermented beans and bean paste. The fruits and vegetables in the fermented fruit and vegetable products include but are not limited to cucumber, carrot, beet, celery and cabbage.

The present disclosure also provides a pharmaceutical preparation, comprising an effective amount of the strain CCFM8631 of Lactobacillus reuteri and a pharmaceutically acceptable adjuvant.

The pharmaceutically acceptable adjuvant is one or more selected from the group consisting of filler, adhesive, wetting agent, disintegrating agent, lubricant, and flavoring agent.

In some embodiments of the present disclosure, the pharmaceutical preparation is a granule, a capsule, a tablet, a pill or an oral liquid.

The beneficial technical effects of the present disclosure are as follows.

The strain CCFM8631 of Lactobacillus reuteri of the present disclosure significantly increases neurotransmitter 5-hydroxytryptamine (5-HT) level in peripheral blood of rat, regulates brain-gut axis, relieves mental illnesses related to metabolic syndrome, for example anxiety, depression and so on, recovers the hormone level, for example testosterone and so on in peripheral blood of rat caused by high-fat high-starch diet, recovers abundances of Blautia genus, Turicibacter genus, Oscillospira genus and Bifidobacterium genus in abnormal intestinal flora of rat caused by high-fat high-starch diet. In addition, strain CCFM8631 of Lactobacillus reuteri has pretty good tolerance to simulated gastrointestinal fluid, and quickly colonizes in intestinal, significantly alleviates pathology damages of tissues, such as liver, duodenum and so on of rat with metabolic syndrome caused by high-fat high-starch diet; significantly improves oral glucose tolerance of rat with metabolic syndrome and decreases the under curve area of glucose tolerance test; significantly increases triglyceride and total cholesterol levels in serum of rat with metabolic syndrome caused by high-fat high-starch diet. The strain CCFM8631 of Lactobacillus reuteri of the present disclosure can be used to prepare health foods or medicines that improve metabolic syndrome, regulates intestinal flora, relieves irritable bowel syndrome, regulates brain-gut axis and alleviates mental illness such as anxiety, depression and so on, which has a pretty wide application prospect.

DESCRIPTION OF MICROBIOLOGICAL PRESERVATION

CCFM8631, classification name: Lactobacillus reuteri, is deposited at China General Microbiological Culture Collection Center (CGMCC, Address: Beijing Institute of Microbiology, Chinese Academy of Sciences, No. 1, Beichen West Road, Chaoyang District, Beijing, China) on Jul. 7, 2017, with an accession number CGMCC 14394.

BRIEF DESCRIPTION OF DRAWINGS

In order to describe the technical solutions in the examples of the present disclosure or the conventional art more clearly, the accompanying drawings used in description of the embodiments or the prior art will be illustrated briefly.

FIG. 1 shows colony morphology of strain CCFM8631 of Lactobacillus reuteri.

FIG. 2 shows effects of strain CCFM8631 of Lactobacillus reuteri on tissue structure of liver in rat with metabolic syndrome.

FIG. 3 shows effects of strain CCFM8631 of Lactobacillus reuteri on tissue structure of duodenum in rat with metabolic syndrome.

FIG. 4 shows effects of strain CCFM8631 of Lactobacillus reuteri on abundances of some intestinal microbes in rat with metabolic syndrome; and there are significant differences (P<0.05) between a, b and c groups.

FIG. 5 shows effects of strain CCFM8631 of Lactobacillus reuteri on oral glucose tolerance in rat with metabolic syndrome; and there are significant differences (P<0.05) between a, b and c groups.

FIG. 6 is a curve showing changes of blood glucose level in oral glucose tolerance test.

FIG. 7 shows the area under the curve (AUC_glucose) in oral glucose tolerance test.

FIG. 8 shows effects of strain CCFM8631 of Lactobacillus reuteri on total cholesterol (TC) in serum of rat with metabolic syndrome; and there are significant differences (P<0.05) between a, b and c groups.

FIG. 9 shows effects of strain CCFM8631 of Lactobacillus reuteri on triglyceride (TG) in serum of rat with metabolic syndrome; and there are significant differences (P<0.05) between a, b and c groups.

FIG. 10 shows effects of strain CCFM8631 of Lactobacillus reuteri on 5-hydroxytryptamine (5-HT) and testosterone levels of rat with metabolic syndrome; and there are significant differences (P<0.05) between a, b and c groups.

DETAILED DESCRIPTION

The present disclosure discloses a Lactobacillus reuteri and use thereof. One of ordinary skill in the art can learn from the contents herein and improve the process parameters appropriately. In particular, it shall be noted that all the similar substitutions and modifications are apparent to one of ordinary skill in the art and are to be considered within the scope of the present invention. The method and product of the present invention have been described with preferred examples. It is apparent that one of the ordinary skill in the art can make change or modify the combination to the method and product of the present invention without departing from the spirit, scope and spirit of the invention, therefore realizing and applying the techniques of the present invention. www

The strain CCFM8631 of Lactobacillus reuteri of the present disclosure has the following biology properties.

(1) Bacterium properties: Gram straining positive, slim rod-shape, no flagella, no spore

(2) Colony properties: convex colonies are formed after 36-hour culture, the edge is not smooth, the color is milky and non-transparent, the surface is moist and smooth, and no pigment is produce. See FIG. 1.

(3) Growth properties: the bacteria are cultured in MRS medium for about 18 hours at constant temperature of 37° C. to reach log phase.

(4) Good tolerance to simulate gastrointestinal fluid.

(5) Significantly improve pathological tissue damages such as liver, duodenum and so on of rat with from metabolic syndrome.

(6) Significantly improve oral glucose tolerance of rat with metabolic syndrome.

(7) Decrease area under the curve in glucose tolerance test.

(8) Regulate the levels of triglyceride and total cholesterol in serum back to normal level.

(9) Increase 5-hydroxytryptamine (5-HT) level in peripheral blood and regulate testosterone to normal level.

(10) Significantly recover abundances of Bifidobacterium genus, Turicibacter genus, Oscillospira genus, Blautia genus and so on in abnormal intestinal flora caused by high-fat high-starch diet.

The strain CCFM8631 of Lactobacillus reuteri of the present disclosure is obtained by the following method.

I. Isolation and Screening of Lactobacillus

(1) 1 g of fresh feces was diluted in gradient, spreaded on Lactobacillus highly selected solid medium plates (0.05 g bromocresol green, 0.02 g vancomycin, 20 g agar per 1 L MRS medium, pH 5.0±0.1), and the plates were incubated at 37° C. for 48-72 hours. The feces were obtained from a 57-year-old healthy female in Changshou Village, Bama Town in Guangxi Province, China.

(2) Morphology of the colonies were observed and recorded, and single colony was picked out and purified by streaking.

(3) The bacteria were cultured at 37° C. for 24 hours in MRS medium, and the colonies obtained were subjected to Gram staining. The morphologies of the colonies were recorded.

(4) The Gram-negative strains and Gram-positive cocci were discarded, the Gram-positive bacilli were selected.

(5) The bacteria were subjected to catalase analyzing, the catalase-positive strains were discarded and catalase-negative strains were retained.

II. Molecular Biological Identification of Lactobacillus reuteri

(1) Genome extraction of single bacterium (according to operation procedures of TIANamp Bacteria DNA kit)

A. The Lactobacillus obtained in Step I was cultured overnight. 1 mL culture was put into a 1.5 mL centrifuge tube and centrifuged at 10,000 rpm (˜11,500×g) for 1 minute. The supernatant was removed as much as possible.

B. 180 μL buffer (20 mg/mL lysozyme solution with 20 mM Tris (pH 8.0), 2 mM Na₂-EDTA, and 1.2% Triton) was added to the bacteria and incubated at 37° C. for more than 30 minutes. (The lysozyme solution should be prepared by dissolving lysozyme dry powder in the buffer, or the lysozyme would be inactive.

C. 20 μL Proteinase K solution was added to the tube and mixed well.

D. 220 μL buffer GB was added, shaken for 15 seconds, and placed at 70° C. for 10 minutes. The solution turned clean. The tube was centrifuged for a few seconds to remove water drops on inner wall of the tube.

E. 220 μL absolute alcohol was added adequately shaken for 15 seconds. Flocculent precipitates maybe appear. The tube was centrifuged for a few seconds to remove water drops on inner wall of the tube.

F. The solution and flocculent precipitate obtained in the last step were put into an adsorption column CB3 (the absorption column was disposed in a collecting tube), and subjected to centrifugation at 12,000 rpm (˜13,400×g) for 30 seconds. The flow-through liquor was discarded, and the adsorption column was put back into the collecting tube.

G. 500 μL of buffer GD (check for absolute alcohol adding before use) was added to the adsorption column CB3. The column was centrifuged at 12,000 rpm (˜13,400×g) for 30 seconds. The flow-through liquor was discarded, and the adsorption column was put back into the collecting tube.

H. 600 μL of washing solution PW (check for absolute alcohol adding before use) was added to the adsorption column CB3. The column was centrifuged at 12,000 rpm (˜13,400×g) for 30 seconds. The flow-through liquor was discarded, and the adsorption column was put back into the collecting tube. This step was repeated once.

I. The adsorption column CB3 was put back into the collecting tube, centrifuged at 12,000 rpm (˜13,400×g) for 2 minutes, and the flow-through liquor was discarded. The adsorption column CB3 was placed at room temperature for a few minutes to let the adsorption column totally dry.

J. The adsorption column CB3 was transferred to a clean centrifugal tube, and 50 to 200 μL of elution buffer TE was dropped to the middle of the adsorption film. The adsorption column was placed at room temperature for 2 to 5 minutes, and then centrifuged at 12,000 rpm (˜13,400×g) for 2 minutes. The eluted solution was collected into a centrifuge tube.

(2) Whole Genome Sequencing

The extracted whole genome was sent to a professional sequencing company and a third-generation sequencer was used to sequence the whole bacterial genome. The sequencing results were subjected to similarity comparison by BLAST software among GeneBank database. The results show that the strain provided by the present disclosure is a Lactobacillus reuteri, but different from known Lactobacillus reuteri, so that it is identified as a new strain. By blastn algorithm, the genome of strain CCFM8631 of Lactobacillus reuteri was compared with that of standard strain DSM20016 of Lactobacillus reuteri (https://wwwncbi.nlm.nih.gov/genome/?term=438), 571 genes in total show differences, which are shown in Table 1.

TABLE 1
Differential genes between strain CCFM8631 of Lactobacillus reuteri and standard strain DSM20016 of Lactobacillus
reuteri
				Corre-					Database	Database
	nr Database			lation			Gene	Gene	Article	Article
Gene ID	Article ID	Note of the Results	Similarity	Length	Mismatching	Gap	Start	End	Start	End	E	Score
TBCP-1560_g_0003	gi|970364679|	oxidoreductase, short chain dehydrogenase/	100	190	0	0	1	570	1	190	6.06E−143	394
	emb|CUR36395.1|	reductase family [Lactobacillus reuteri]
TBCP-1560_g_0004	gi|512154930|	short chain dehydrogenase [Lactobacillus	100	44	0	0	1	132	1	44	4.35E−25	85.5
	gb|AGN98213.1|	reuteri I5007]
TBCP-1560_g_0019	gi|970370732|	Transcriptional regulator, XRE family [Lactobacillus	97.059	34	1	0	1	102	1	34	1.46E−17	69.7
	emb|CUR40704.1|	reuteri]
TBCP-1560_g_0020	gi|953265103|	Insertion sequence IS232 putative ATP-b	100	245	0	0	1	735	1	245	0	504
	emb|CUU12464.1|	inding protein [Lactobacillus reuteri ATCC
		53608]
TBCP-1560_g_0023	gi|512154946|	hypothetical protein LRI_0020 [Lactobacillus	100	31	0	0	95	3	7	37	4.49E−16	62
	gb|AGN98229.1|	reuteri I5007]
TBCP-1560_g_0038	gi|518081627|	sulfurtransferase [Lactobacillus reuteri]	100	258	0	0	1	774	1	258	0	503
	ref|WP_019251835.1|
TBCP-1560_g_0051	gi|948618354|	OsmC family protein [Lactobacillus reuteri	100	142	0	0	1	426	19	160	3.75E−105	296
	gb|KRK50363.1|	DSM 20016]
TBCP-1560_g_0052	gi|1017201634|	lactate dehydrogenase [Lactobacillus reuteri]	99.698	331	1	0	1	993	1	331	0	644
	ref|WP_063164307.1|
TBCP-1560_g_0054	gi|489766316|	hypothetical protein [Lactobacillus reuteri]	100	73	0	0	1	219	1	73	3.14E−41	128
	ref|WP_003670244.1|
TBCP-1560_g_0069	gi|489771585|	GTP pyrophosphokinase [Lactobacillus reuteri]	100	206	0	0	1	618	1	206	1.73E−156	430
	ref|WP_003675493.1|
TBCP-1560_g_0080	gi|489771566|	guanosine monophosphate reductase [Lactobacillus	100	324	0	0	1	972	1	324	0	674
	ref|WP_003675474.1|	reuteri]
TBCP-1560_g_0084	gi|489771562|	hypothetical protein [Lactobacillus reuteri]	100	128	0	0	1	384	1	128	3.65E−93	263
	ref|WP_003675470.1|
TBCP-1560_g_0095	gi|489771550|	carbamoyl phosphate synthase large subunit	100	1056	0	0	1	3168	1	1056	0	2122
	ref|WP_003675458.1|	[Lactobacillus reuteri]
TBCP-1560_g_0109	gi|489771532|	DNA-binding response regulator [Lactobacillus	100	226	0	0	1	678	1	226	5.55E−155	428
	ref|WP_003675440.1|	reuteri]
TBCP-1560_g_0114	gi|489771525|	transcriptional regulator [Lactobacillus reuteri]	100	197	0	0	1	591	1	197	4.60E−149	410
	ref|WP_003675433.1|
TBCP-1560_g_0115	gi|489771524|	hypothetical protein [Lactobacillus reuteri]	100	226	0	0	1	678	1	226	6.77E−170	466
	ref|WP_003675432.1|
TBCP-1560_g_0116	gi|489771523|	NAD-dependent protein deacetylase [Lactobacillus	100	324	0	0	1	972	1	324	0	645
	ref|WP_003675431.1|	reuteri]
TBCP-1560_g_0120	gi|512155047|	acetyltransferase [Lactobacillus reuteri I5007]	100	176	0	0	1	528	1	176	1.40E−111	314
	gb|AGN98330.1|
TBCP-1560_g_0121	gi|489761202|	hypothetical protein [Lactobacillus reuteri]	98.507	67	1	0	1	201	1	67	1.44E−44	136
	ref|WP_003665145.1|
TBCP-1560_g_0122	gi|489771517|	cell surface protein [Lactobacillus reuteri]	100	286	0	0	1	858	1	286	0	584
	ref|WP_003675425.1|
TBCP-1560_g_0123	gi|737175530|	phenolic acid decarboxylase padC [Lactobacillus	100	179	0	0	1	537	1	179	3.28E−134	371
	ref|WP_035161724.1|	reuteri]
TBCP-1560_g_0125	gi|337728644|	putative NAD binding protein, possible epimerase/	100	208	0	0	1	624	1	208	7.71E−155	426
	emb|CCC03755.1|	dehydratase [Lactobacillus reuteri
		ATCC 53608]
TBCP-1560_g_0134	gi|512738680|	NADH-flavin reductase-like protein [Lactobacillus	100	214	0	0	1	642	1	214	5.25E−162	444
	ref|WP_016496408.1|	reuteri]
TBCP-1560_g_0135	gi|489765480|	HesB/YadR/YfhF-family protein [Lactobacillus	100	120	0	0	1	360	4	123	1.08E−87	249
	ref|WP_003669411.1|	reuteri]
TBCP-1560_g_0136	gi|489765479|	glutamine amidotransferase [Lactobacillus	100	223	0	0	1	669	1	223	5.25E−170	466
	ref|WP_003669410.1|	reuteri]
TBCP-1560_g_0139	gi|489771502|	diguanylate phosphodiesterase [Lactobacillus	100	211	0	0	1	633	1	211	2.57E−158	436
	ref|WP_003675410.1|	reuteri]
TBCP-1560_g_0148	gi|512738689|	hypothetical protein [Lactobacillus reuteri]	95.652	115	5	0	1	345	1	115	6.55E−67	207
	ref|WP_016496417.1|
TBCP-1560_g_0154	gi|518083879|	NAD-dependent dehydratase [Lactobacillus	100	48	0	0	1	144	1	48	6.74E−29	100
	ref|WP_019254087.1|	reuteri]
TBCP-1560_g_0156	gi|489771478|	GCN5 family N-acetyltransferase [Lactobacillus	100	145	0	0	1	435	1	145	3.98E−108	303
	ref|WP_003675387.1|	reuteri]
TBCP-1560_g_0158	gi|489761160|	cytochrome b5 [Lactobacillus reuteri]	100	72	0	0	1	216	1	72	2.40E−47	143
	ref|WP_003665104.1|
TBCP-1560_g_0159	gi|489771474|	cytochrome b5 [Lactobacillus reuteri]	100	81	0	0	1	243	1	81	3.19E−57	169
	ref|WP_003675383.1|
TBCP-1560_g_0161	gi|337728616|	hypothetical protein LRATCC53608_0972	100	38	0	0	1	114	1	38	6.70E−21	74.7
	emb|CCC03724.1|	[Lactobacillus reuteri ATCC 53608]
TBCP-1560_g_0180	gi|489771454|	TetR family transcriptional regulator [Lactobacillus	100	170	0	0	1	510	1	170	3.30E−127	353
	ref|WP_003675363.1|	reuteri]
TBCP-1560_g_0184	gi|489771446|	hypothetical protein [Lactobacillus reuteri]	100	78	0	0	1	234	1	78	2.35E−55	164
	ref|WP_003675355.1|
TBCP-1560_g_0190	gi|489771434|	hypothetical protein [Lactobacillus reuteri]	100	206	0	0	1	618	1	206	5.91E−156	429
	ref|WP_003675343.1|
TBCP-1560_g_0192	gi|489771430|	histidine--tRNA ligase [Lactobacillus reuteri]	100	425	0	0	1	1275	1	425	0	836
	ref|WP_003675339.1|
TBCP-1560_g_0197	gi|227186376|	hypothetical protein HMPREF0534_0218	94.231	52	3	0	1	156	1	52	1.84E−30	99.8
	gb|EEI66447.1|	[Lactobacillus reuteri CF48-3A]
TBCP-1560_g_0199	gi|489765400|	hypothetical protein [Lactobacillus reuteri]	99.541	218	1	0	1	654	1	218	1.28E−165	454
	ref|WP_003669331.1|
TBCP-1560_g_0200	gi|526246046|	hypothetical protein [Lactobacillus reuteri]	100	39	0	0	1	117	1	39	4.20E−17	67
	ref|WP_020843400.1|
TBCP-1560_g_0208	gi|512155129|	C4-dicarboxylate anaerobic carrier [Lactobacillus	98.592	71	1	0	1	213	1	71	2.23E−48	146
	gb|AGN98412.1|	reuteri I5007]
TBCP-1560_g_0216	gi|489771396|	RNA pseudouridine synthase [Lactobacillus	100	306	0	0	1	918	1	306	0	630
	ref|WP_003675305.1|	reuteri]
TBCP-1560_g_0217	gi|526246038|	membrane protein [Lactobacillus reuteri]	99.203	251	2	0	1	753	1	251	5.51E−174	478
	ref|WP_020843392.1|
TBCP-1560_g_0220	gi|512738724|	signal peptide [Lactobacillus reuteri]	99.914	1166	1	0	1	3498	1	1166	0	2135
	ref|WP_016496451.1|
TBCP-1560_g_0227	gi|1017201665|	patatin family protein [Lactobacillus reuteri]	100	74	0	0	1	222	1	74	2.62E−50	151
	ref|WP_063164338.1|
TBCP-1560_g_0229	gi|754210084|	hypothetical protein [Lactobacillus reuteri]	100	100	0	0	1	300	1	100	2.00E−70	204
	ref|WP_041821572.1|
TBCP-1560_g_0231	gi|489771374|	membrane protein [Lactobacillus reuteri]	99.614	259	1	0	1	777	1	259	0	535
	ref|WP_003675283.1|
TBCP-1560_g_0237	gi|512155158|	hypothetical protein LRI_0232 [Lactobacilitis	100	61	0	0	1	183	1	61	2.47E−41	127
	gb|AGN98441.1|	reuteri I5007]
TBCP-1560_g_0240	gi|489761065|	hypothetical protein [Lactobacillus reuteri]	100	106	0	0	1	318	1	106	3.15E−76	219
	ref|WP_003665009.1|
TBCP-1560_g_0241	gi|970363723|	Glutathione-regulated potassium-efflux system	100	227	0	0	1	681	1	227	3.80E−149	413
	emb|CUR37285.1|	ancillary protein KefG [Lactobacillus
		reuteri]
TBCP-1560_g_0242	gi|337728551|	hypothetical protein LRATCC53608_0905	100	57	0	0	1	171	1	57	5.59E−38	119
	emb|CCC03657.1|	[Lactobacillus reuteri ATCC 53608]
TBCP-1560_g_0245	gi|489771350|	ATPase [Lactobacillus reuteri]	99.738	381	1	0	1	1143	1	381	0	771
	ref|WP_003675259.1|
TBCP-1560_g_0246	gi|489771348|	peptidase [Lactobacillus reuteri]	99.535	430	2	0	1	1290	1	430	0	850
	ref|WP_003675257.1|
TBCP-1560_g_0248	gi|489771344|	peptidase M13 [Lactobacillus reuteri]	99.685	634	2	0	1	1902	1	634	0	1298
	ref|WP_003675253.1|
TBCP-1560_g_0249	gi|512738735|	transcriptional regulator [Lactobacillus reuteri]	99.528	212	1	0	1	636	1	212	5.98E−157	432
	ref|WP_016496462.1|
TBCP-1560_g_0250	gi|489771340|	RNase III inhibitor [Lactobacillus reuteri]	99.401	167	1	0	1	501	1	167	2.83E−124	345
	ref|WP_003675249.1|
TBCP-1560_g_0255	gi|512156691|	transposase [Lactobacillus reuteri I5007]	96.552	29	1	0	1	87	14	42	1.86E−15	60.5
	gb|AGN99974.1|
TBCP-1560_g_0276	gi|970366323|	Proton/glutamate symport protein @ Sodium/	99.771	437	1	0	1	1311	1	437	0	842
	emb|CUR42817.1|	glutamate symport protein [Lactobacillus
		reuteri]
TBCP-1560_g_0279	gi|512738751|	low temperature requirement protein LtrA	100	123	0	0	4	372	2	124	1.04E−86	247
	ref|WP_016496478.1|	[Lactobacillus reuteri]
TBCP-1560_g_0280	gi|489765927|	hypothetical protein [Lactobacillus reuteri]	100	175	0	0	1	525	35	209	1.27E−113	320
	ref|WP_003669856.1|
TBCP-1560_g_0291	gi|512738759|	ABC transporter ATP-binding protein [Lactobacillus	99.303	287	2	0	1	861	1	287	0	586
	ref|WP_016496485.1|	reuteri]
TBCP-1560_g_0292	gi|337728515|	hypothetical protein LRATCC53608_0867	99.425	174	1	0	1	522	5	178	1.84E−127	354
	emb|CCC03619.1|	[Lactobacillus reuteri ATCC 53608]
TBCP-1560_g_0293	gi|659900074|	hypothetical protein LR3_04370 [Lactobacillus	100	193	0	0	1	579	1	193	1.28E−122	343
	gb|KEK14882.1|	reuteri]
TBCP-1560_g_0294	gi|489760987|	MarR family transcriptional regulator [Lactobacillus	99.333	150	1	0	1	450	1	150	1.06E−107	302
	ref|WP_003664931.1|	reuteri]
TBCP-1560_g_0295	gi|489765281|	hypothetical protein [Lactobacillus reuteri]	100	241	0	0	1	723	1	241	6.83E−175	479
	ref|WP_003669212.1|
TBCP-1560_g_0298	gi|754210095|	hypothetical protein [Lactobacillus reuteri]	99.333	300	2	0	1	900	12	311	0	506
	ref|WP_041821582.1|
TBCP-1560_g_0300	gi|489760978|	glycerol transporter [Lactobacillus reuteri]	100	235	0	0	1	705	1	235	2.25E−169	465
	ref|WP_003664922.1|
TBCP-1560_g_0302	gi|512738768|	ABC transporter-like protein [Lactobacillus	99.655	580	2	0	1	1740	1	580	0	1146
	ref|WP_016496494.1|	reuteri]
TBCP-1560_g_0307	gi|489771190|	GntR family transcriptional regulator [Lactobacillus	100	243	0	0	1	729	1	243	1.74E−179	491
	ref|WP_003675099.1|	reuteri]
TBCP-1560_g_0308	gi|489771188|	phosphoketolase [Lactobacillus reuteri]	100	803	0	0	1	2409	1	803	0	1671
	ref|WP_003675097.1|
TBCP-1560_g_0311	gi|489771184|	alpha/beta hydrolase [Lactobacillus reuteri]	100	248	0	0	1	744	1	248	0	509
	ref|WP_003675093.1|
TBCP-1560_g_0313	gi|489771181|	transcriptional regulator [Lactobacillus reuteri]	100	300	0	0	1	900	1	300	0	595
	ref|WP_003675090.1|
TBCP-1560_g_0316	gi|489771178|	lipoate--protein ligase A [Lactobacillus reuteri]	100	337	0	0	1	1011	1	337	0	699
	ref|WP_003675087.1|
TBCP-1560_g_0318	gi|489765148|	hypothetical protein [Lactobacillus reuteri]	100	152	0	0	1	456	1	152	9.65E−111	310
	ref|WP_003669080.1|
TBCP-1560_g_0320	gi|489771175|	PTS galactitol transporter subunit IIC [Lactobacillus	100	470	0	0	1	1410	1	470	0	895
	ref|WP_003675084.1|	reuteri]
TBCP-1560_g_0324	gi|737175460|	alpha/beta hydrolase [Lactobacillus reuteri]	99.693	326	1	0	1	978	1	326	0	634
	ref|WP_035161655.1|
TBCP-1560_g_0325	gi|489760939|	membrane protein [Lactobacillus reuteri]	100	118	0	0	1	354	1	118	6.50E−68	199
	ref|WP_003664884.1|
TBCP-1560_g_0334	gi|489771154|	ferredoxin--NADP(+) reductase [Lactobacillus	100	332	0	0	1	996	1	332	0	681
	ref|WP_003675063.1|	reuteri]
TBCP-1560_g_0346	gi|489765111|	spermidine/purescine ABC transporter permease	100	272	0	0	1	816	1	272	0	516
	ref|WP_003669043.1|	[Lactobacillus reuteri]
TBCP-1560_g_0347	gi|489771138|	spermidine/putrescine ABC transporter substrate-	100	357	0	0	1	1071	1	357	0	687
	ref|WP_003675047.1|	binding protein [Lactobacillus reuteri]
TBCP-1560_g_0352	gi|489771133|	guanylate kinase [Lactobacillus reuteri]	100	190	0	0	1	570	1	190	4.51E−143	395
	ref|WP_003675042.1|
TBCP-1560_g_0354	gi|489760888|	hypothetical protein [Lactobacillus reuteri]	99.206	126	1	0	1	378	1	126	4.36E−46	147
	ref|WP_003664833.1|
TBCP-1560_g_0361	gi|953264637|	CsbD family protein [Lactobacillus reuteri	100	61	0	0	1	183	1	61	1.12E−08	45.4
	emb|CUU11948.1|	ATCC 53608]
TBCP-1560_g_0370	gi|489771105|	type I pantothenate kinase [Lactobacillus	100	307	0	0	1	921	1	307	0	630
	ref|WP_003675014.1|	reuteri]
TBCP-1560_g_0380	gi|337728374|	conserved hypothetical protein [Lactobacillus	100	204	0	0	1	612	4	207	4.94E−152	419
	emb|CCC03475.1|	reuteri ATCC 53608]
TBCP-1560_g_0381	gi|489760823|	peptidase M10 [Lactobacillus reuteri]	100	231	0	0	1	693	1	231	2.95E−138	386
	ref|WP_003664768.1|
TBCP-1560_g_0384	gi|194454116|	hypothetical protein Lreu23DRAFT_4532	92.683	41	3	0	1	123	1	41	2.22E−22	78.6
	gb|EDX43013.1|	[Lactobacillus reuteri 100-23]
TBCP-1560_g_0387	gi|512155310|	membrane protein [Lactobacillus reuteri	100	369	0	0	1	1107	1	369	0	515
	gb|AGN98593.1|	I5007]
TBCP-1560_g_0388	gi|489760812|	elongation factor P [Lactobacillus reuteri]	100	185	0	0	1	555	1	185	3.22E−136	377
	ref|WP_003664757.1|
TBCP-1560_g_0389	gi|489760811|	hypothetical protein [Lactobacillus reuteri]	97.714	350	8	0	1	1050	1	350	0	573
	ref|WP_003664756.1|
TBCP-1560_g_0394	gi|970366169|	FIG00746573: hypothetical protein [Lactobacillus	100	338	0	0	1	1014	1	338	0	699
	emb|CUR43008.1|	reuteri]
TBCP-1560_g_0396	gi|737167860|	hypothetical protein [Lactobacillus reuteri]	89.313	131	14	0	1	393	1	131	1.12E−84	242
	ref|WP_035154108.1|
TBCP-1560_g_0399	gi|1016922021|	phosphate ABC transporter substrate-binding	100	290	0	0	1	870	1	290	0	591
	gb|AMY14452.1|	protein [Lactobacillus reuteri]
TBCP-1560_g_0400	gi|489771084|	phosphate ABC transporter permease sub	99.666	299	1	0	1	897	1	299	0	530
	ref|WP_003674993.1|	unit PstC [Lactobacillus reuteri]
TBCP-1560_g_0403	gi|227184997|	phosphate transport system regulatory protein	100	234	0	0	1	702	10	243	4.44E−174	477
	gb|EEI65068.1|	PhoU [Lactobacillus reuteri CF48-
		3A]
TBCP-1560_g_0405	gi|970368823|	FIG00748601: hypothetical protein [Lactobacillus	100	287	0	0	1	861	1	287	0	578
	emb|CUR38312.1|	reuteri]
TBCP-1560_g_0406	gi|512155320|	hypothetical protein LRI_0394 [Lactobacillus	100	48	0	0	1	144	1	48	3.21E−28	93.6
	gb|AGN98603.1|	reuteri I5007]
TBCP-1560_g_0407	gi|512155321|	glycosyl transferase family protein [Lactobacillus	100	227	0	0	1	681	1	227	1.65E−160	442
	gb|AGN98604.1|	reuteri I5007]
TBCP-1560_g_0410	gi|512155324|	glycosyltransferase [Lactobacillus reuteri	100	191	0	0	1	573	1	191	5.67E−120	337
	gb|AGN98607.1|	I5007]
TBCP-1560_g_0411	gi|512738819|	glycoside hydrolase [Lactobacillus reuteri]	100	379	0	0	1	1137	1	379	0	798
	ref|WP_016496544.1|
TBCP-1560_g_0414	gi|512738822|	potassium uptake protein [Lactobacillus reuteri]	99.776	447	1	0	1	1341	1	447	0	823
	ref|WP_016496547.1|
TBCP-1560_g_0416	gi|970368831|	FIG00748017: hypothetical protein [Lactobacillus	100	164	0	0	52	543	18	181	7.57E−118	330
	emb|CUR38320.1|	reuteri]
TBCP-1560_g_0420	gi|970367179|	Cystathionine beta-synthase [Lactobacillus	100	304	0	0	1	912	1	304	0	531
	emb|CUR39802.1|	reuteri]
TBCP-1560_g_0423	gi|970367177|	Inner membrane protein [Lactobacillus reuteri]	100	339	0	0	1	1017	4	342	0	585
	emb|CUR39800.1|
TBCP-1560_g_0425	gi|512738831|	esterase [Lactobacillus reuteri]	100	238	0	0	1	714	1	238	4.41E−180	492
	ref|WP_016496555.1|
TBCP-1560_g_0430	gi|489771049|	hypothetical protein [Lactobacillus reuteri]	100	444	0	0	1	1332	1	444	0	917
	ref|WP_003674958.1|
TBCP-1560_g_0431	gi|489771047|	MFS transporter permease [Lactobacillus	100	405	0	0	1	1215	1	405	0	664
	ref|WP_003674956.1|	reuteri]
TBCP-1560_g_0435	gi|512155348|	D-alanyl-D-alanine carboxypeptidase [Lactobacillus	100	417	0	0	1	1251	1	417	0	768
	gb|AGN98631.1|	reuteri I5007]
TBCP-1560_g_0440	gi|512738842|	transposase [Lactobacillus reuteri]	100	386	0	0	1	1158	1	386	0	806
	ref|WP_016496566.1|
TBCP-1560_g_0441	gi|953264694|	hypothetical protein LRATCC53608_0483	99.643	561	2	0	1	1683	1	561	0	1136
	emb|CUU12019.1|	[Lactobacillus reuteri ATCC 53608]
TBCP-1560_g_0443	gi|489771033|	peptidyl-prolyl cis-trans isomerase [Lactobacillus	100	186	0	0	1	558	1	186	3.90E−128	357
	ref|WP_003674942.1|	reuteri]
TBCP-1560_g_0445	gi|1017201710|	NAD-dependent malic enzyme [Lactobacillus	100	542	0	0	1	1626	1	542	0	1083
	ref|WP_063164383.1|	reuteri]
TBCP-1560_g_0447	gi|489771029|	flavocytochrome c [Lactobacillus reuteri]	100	464	0	0	1	1392	1	464	0	944
	ref|WP_003674938.1|
TBCP-1560_g_0450	gi|227185979|	putative sugar-binding domain protein [Lactobacillus	98.403	313	5	0	1	939	55	367	0	641
	gb|EEI66050.1|	reuteri CF48-3A]
TBCP-1560_g_0452	gi|227185977|	[citrate (pro-3S)-lyase] ligase [Lactobacillus	99.424	347	2	0	1	1041	13	359	0	712
	gb|EEI66048.1|	reuteri CF48-3A]
TBCP-1560_g_0453	gi|489766774|	citrate lyase ACP [Lactobacillus reuteri]	100	97	0	0	1	291	1	97	3.27E−67	196
	ref|WP_003670701.1|
TBCP-1560_g_0454	gi|489766772|	citrate (pro-3S)-lyase subunit beta [Lactobacillus	100	301	0	0	1	903	1	301	0	598
	ref|WP_003670699.1|	reuteri]
TBCP-1560_g_0456	gi|518082469|	triphosphoribosyl-dephospho-CoA synthase	98.737	475	6	0	1	1425	1	475	0	966
	ref|WP_019252677.1|	[Lactobacillus reuteri]
TBCP-1560_g_0457	gi|518082470|	hydrolase [Lactobacillus reuteri]	98.099	263	5	0	1	789	1	263	0	538
	ref|WP_019252678.1|
TBCP-1560_g_0459	gi|489760694|	LysR family transcriptional regulator [Lactobacillus	100	295	0	0	1	885	1	295	0	599
	ref|WP_003664640.1|	reuteri]
TBCP-1560_g_0460	gi|489764917|	membrane protein [Lactobacillus reuteri]	99.341	455	3	0	1	1365	1	455	0	782
	ref|WP_003668849.1|
TBCP-1560_g_0461	gi|737167582|	uracil-DNA glycosylase [Lactobacillus reuteri]	99.078	217	2	0	1	651	1	217	2.67E−161	443
	ref|WP_035153834.1|
TBCP-1560_g_0463	gi|489771024|	alpha/beta hydrolase [Lactobacillus reuteri]	99.694	327	1	0	1	981	1	327	0	679
	ref|WP_003674933.1|
TBCP-1560_g_0466	gi|336448233|	pseudouridine synthase, RluA family [Lactobacillus	100	290	0	0	1	870	26	315	0	604
	gb|AEI56848.1|	reuteri SD2112]
TBCP-1560_g_0469	gi|489764899|	MerR family transcriptional regulator [Lactobacillus	100	148	0	0	1	444	1	148	1.45E−107	301
	ref|WP_003668831.1|	reuteri]
TBCP-1560_g_0470	gi|489771016|	hypothetical protein [Lactobacillus reuteri]	99.13	115	1	0	1	345	1	115	1.63E−82	236
	ref|WP_003674926.1|
TBCP-1560_g_0471	gi|489771015|	RNase J family beta-CASP ribonuclease	100	618	0	0	1	1854	1	618	0	1281
	ref|WP_003674925.1|	[Lactobacillus reuteri]
TBCP-1560_g_0472	gi|970364104|	FIG00743019: hypothetical protein [Lactobacillus	100	395	0	0	1	1185	1	395	0	757
	emb|CUR36934.1|	reuteri]
TBCP-1560_g_0477	gi|489764884|	universal stress protein UspA [Lactobacillus	100	158	0	0	1	474	1	158	2.14E−115	322
	ref|WP_003668816.1|	reuteri]
TBCP-1560_g_0479	gi|489771009|	membrane protein [Lactobacillus reuteri]	100	230	0	0	1	690	1	230	1.11E−144	402
	ref|WP_003674919.1|
TBCP-1560_g_0482	gi|489760658|	cold-shock protein [Lactobacillus reuteri]	100	66	0	0	1	198	1	66	8.39E−44	134
	ref|WP_003664604.1|
TBCP-1560_g_0485	gi|489764871|	phosphatase [Lactobacillus reuteri]	100	224	0	0	1	672	1	224	1.46E−159	439
	ref|WP_003668803.1|
TBCP-1560_g_0486	gi|489771002|	N-acetyltransferase GCN5 [Lactobacillus	100	151	0	0	1	453	1	151	9.53E−112	312
	ref|WP_003674912.1|	reuteri]
TBCP-1560_g_0489	gi|970366045|	membrane protein [Lactobacillus reuteri]	93.627	204	11	1	7	612	34	237	1.97E−144	400
	emb|CUR43096.1|
TBCP-1560_g_0490	gi|512155402|	membrane protein [Lactobacillus reuteri	100	48	0	0	1	144	1	48	4.28E−29	101
	gb|AGN9868 5.1|	I5007]
TBCP-1560_g_0491	gi|512155403|	membrane protein [Lactobacillus reuteri	100	47	0	0	1	141	1	47	3.79E−30	98.6
	gb|AGN98686.1|	I5007]
TBCP-1560_g_0493	gi|227071188|	transcriptional repressor of CtsR [Lactobacillus	100	155	0	0	1	465	13	167	3.41E−98	279
	gb|EEI09504.1|	reuteri MM2-3]
TBCP-1560_g_0500	gi|489760630|	30S ribosomal protein S12 [Lactobacillus	100	139	0	0	1	417	1	139	5.65E−88	251
	ref|WP_003664576.1|	reuteri]
TBCP-1560_g_0501	gi|489764856|	30S ribosomal protein S7 [Lactobacillus	100	156	0	0	1	468	1	156	1.98E−116	325
	ref|WP_003668788.1|	reuteri]
TBCP-1560_g_0504	gi|489760623|	30S ribosomal protein S10 [Lactobacillus	100	102	0	0	1	306	1	102	1.43E−70	204
	ref|WP_003664569.1|	reuteri]
TBCP-1560_g_0505	gi|489760621|	50S ribosomal protein L3 [Lactobacillus	100	219	0	0	1	657	1	219	1.69E−148	411
	ref|WP_003664567.1|	reuteri]
TBCP-1560_g_0506	gi|489760620|	50S ribosomal protein L4 [Lactobacillus	100	207	0	0	1	621	1	207	1.00E−140	390
	ref|WP_003664566.1|	reuteri]
TBCP-1560_g_0507	gi|489760618|	50S ribosomal protein L23 [Lactobacillus	100	98	0	0	1	294	1	98	1.39E−54	164
	ref|WP_003664564.1|	reuteri]
TBCP-1560_g_0508	gi|489760617|	50S ribosomal protein L2 [Lactobacillus	99.644	281	1	0	1	843	1	281	0	566
	ref|WP_003664563.1|	reuteri]
TBCP-1560_g_0509	gi|489760614|	30S ribosomal protein S19 [Lactobacillus	100	92	0	0	1	276	1	92	1.12E−64	189
	ref|WP_003664560.1|	reuteri]
TBCP-1560_g_0510	gi|489770978|	50S ribosomal protein L22 [Lactobacillus	100	115	0	0	1	345	1	115	4.01E−78	224
	ref|WP_003674888.1|	reuteri]
TBCP-1560_g_0511	gi_489760610|	30S ribosomal protein S3 [Lactobacillus	99.548	221	1	0	1	663	1	221	4.35E−147	407
	ref|WP_003664556.1|	reuteri]
TBCP-1560_g_0513	gi_489760606|	MULTISPECIES: 50S ribosomal protein	100	68	0	0	1	204	1	68	3.61E−43	132
	ref|WP_003664552.1|	L29 [Lactobacillus]
TBCP-1560_g_0514	gi|489760605|	30S ribosomal protein S17 [Lactobacillus	100	88	0	0	1	264	1	88	4.82E−61	179
	ref|WP_003664551.1|	reuteri]
TBCP-1560_g_0516	gi|489760603|	50S ribosomal protein L24 [Lactobacillus	100	102	0	0	1	306	1	102	3.16E−69	201
	ref|WP_003664549.1|	reuteri]
TBCP-1560_g_0517	gi|489764852|	50S ribosomal protein L5 [Lactobacillus	100	180	0	0	1	540	1	180	2.58E−132	367
	ref|WP_003668784.1|	reuteri]
TBCP-1560_g_0518	gi|489760600|	30S ribosomal protein S8 [Lactobacillus	100	132	0	0	1	396	1	132	3.69E−94	266
	ref|WP_003664546.1|	reuteri]
TBCP-1560_g_0522	gi|489760595|	50S ribosomal protein L30 [Lactobacillus	100	60	0	0	1	180	1	60	9.81E−39	121
	ref|WP_003664541.1|	reuteri]
TBCP-1560_g_0525	gi|970368280|	Adenylate kinase [Lactobacillus reuteri]	100	219	0	0	1	657	1	219	4.25E−165	453
	emb|CUR38822.1|
TBCP-1560_g_0526	gi|489770966|	translation initiation factor IF-1 [Lactobacillus	98.611	72	1	0	1	216	1	72	6.65E−48	145
	ref|WP_003674876.1|	reuteri]
TBCP-1560_g_0527	gi|489760589|	50S ribosomal protein L36 [Lactobacillus	100	39	0	0	1	117	1	39	1.68E−22	78.6
	ref|WP_003664535.1|	reuteri]
TBCP-1560_g_0534	gi|489770957|	cobalt ABC transporter ATP-binding protein	100	267	0	0	1	801	1	267	8.36E−167	461
	ref|WP_003674867.1|	[Lactobacillus reuteri]
TBCP-1560_g_0535	gi|489770955|	tRNA pseudouridine(38, 39, 40) synthase TruA	100	256	0	0	1	768	1	256	0	529
	ref|WP_003674865.1|	[Lactobacillus reuteri]
TBCP-1560_g_0537	gi|489764839|	30S ribosomal protein S9 [Lactobacillus	100	133	0	0	1	399	1	133	1.04E−94	268
	ref|WP_003668771.1|	reuteri]
TBCP-1560_g_0540	gi|489770952|	acetoin reductase [Lactobacillus reuteri]	99.611	257	1	0	1	771	1	257	0	515
	ref|WP_003674862.1|
TBCP-1560_g_0542	gi|489770949|	xanthine phosphoribosyltransferase [Lactobacillus	100	191	0	0	1	573	1	191	1.21E−129	361
	ref|WP_003674859.1|	reuteri]
TBCP-1560_g_0543	gi|489770948|	phosphoribosylaminoimidazole carboxylase	99.735	377	1	0	1	1131	1	377	0	773
	ref|WP_003674858.1|	[Lactobacillus reuteri]
TBCP-1560_g_0544	gi|489760563|	ATP-dependent DNA helicase PcrA [Lactobacillus	99.868	757	1	0	1	2271	1	757	0	1530
	ref|WP_003664509.1|	reuteri]
TBCP-1560_g_0546	gi|489770944|	CamS family sex pheromone protein [Lactobacillus	99.73	371	1	0	1	1113	1	371	0	681
	ref|WP_003674854.1|	reuteri]
TBCP-1560_g_0547	gi|489770942|	asparaginyl/glutamyl-tRNA amidotransferase	100	105	0	0	1	315	1	105	2.86E−73	211
	ref|WP_003674852.1|	subunit C [Lactobacillus reuteri]
TBCP-1560_g_0553	gi|489764819|	23S rRNA (uracil-5-)-methyltransferase RumA	98.954	478	5	0	1	1434	1	478	0	974
	ref|WP_003668751.1|	[Lactobacillus reuteri]
TBCP-1560_g_0560	gi|489767756|	preprotein translocase subunit TatB [Lactobacillus	89.011	91	10	0	1	273	88	178	8.68E−56	169
	ref|WP_003671680.1|	reuteri]
TBCP-1560_g_0562	gi|489767761|	hypothetical protein [Lactobacillus reuteri]	100	103	0	0	1	309	1	103	9.66E−63	186
	ref|WP_003671685.1|
TBCP-1560_g_0564	gi|512155472|	hypothetical protein LRI_0546 [Lactobacillus	99.213	127	1	0	1	381	1	127	3.88E−92	261
	gb|AGN98755.1|	reuteri I5007]
TBCP-1560_g_0567	gi|970364147|	Lead, cadmium, zinc and mercury transporting	99.21	633	5	0	1	1899	1	633	0	1215
	emb|CUR36896.1|	ATPase; Copper-translocating P-type
		ATPase [Lactobacillus reuteri]
TBCP-1560_g_0568	gi|489767767|	MULTISPECIES: copper-binding protein [Lactobacillus]	100	76	0	0	1	228	1	76	4.09E−51	153
	ref|WP_003671691.1|
TBCP-1560_g_0569	gi|489770925|	DNA-binding protein [Lactobacillus reuteri]	100	181	0	0	1	543	1	181	3.88E−132	366
	ref|WP_003674835.1|
TBCP-1560_g_0570	gi|953264799|	CRP/FNR family transcriptional regulator	99.052	211	2	0	1	633	4	214	8.52E−154	424
	emb|CUU12130.1|	[Lactobacillus reuteri ATCC 53608]
TBCP-1560_g_0574	gi|512155481|	DNA-binding protein [Lactobacillus reuteri	98.876	89	1	0	1	267	1	89	3.86E−60	177
	gb|AGN98764.1|	I5007]
TBCP-1560_g_0575	gi|512155482|	DNA-binding protein [Lactobacillus reuteri	100	39	0	0	76	192	1	39	3.82E−23	81.6
	gb|AGN98765.1|	I5007]
TBCP-1560_g_0583	gi|337728224|	conserved hypothetical protein [Lactobacillus	100	441	0	0	1	1323	1	441	0	904
	emb|CCC03316.1|	reuteri ATCC 53608]
TBCP-1560_g_0584	gi|489770905|	hypothetical protein [Lactobacillus reuteri]	100	237	0	0	1	711	1	237	1.80E−179	491
	ref|WP_003674815.1|
TBCP-1560_g_0586	gi|489770902|	nitroreductase [Lactobacillus reuteri]	100	217	0	0	1	651	1	217	1.28E−166	456
	ref|WP_003674812.1|
TBCP-1560_g_0593	gi|489770891|	GCN5 family N-acetyltransferase [Lactobacillus	100	169	0	0	1	507	1	169	8.64E−129	357
	ref|WP_003674801.1|	reuteri]
TBCP-1560_g_0596	gi|489770885|	N-acetyltransferase GCN5 [Lactobacillus	100	151	0	0	1	453	1	151	9.54E−101	285
	ref|WP_003674795.1|	reuteri]
TBCP-1560_g_0598	gi|489770881|	glutamate-cysteine ligase [Lactobacillus	100	509	0	0	1	1527	1	509	0	1053
	ref|WP_003674791.1|	reuteri]
TBCP-1560_g_0600	gi|489770878|	Rossman fold protein, TIGR00730 family	99.476	191	1	0	1	573	1	191	5.66E−144	397
	ref|WP_003674788.1|	[Lactobacillus reuteri]
TBCP-1560_g_0604	gi|489770875|	helicase [Lactobacillus reuteri]	99.307	433	3	0	1	1299	1	433	0	846
	ref|WP_003674785.1|
TBCP-1560_g_0609	gi|489764752|	type I methionyl aminopeptidase [Lactobacillus	100	285	0	0	1	855	1	285	0	524
	ref|WP_003668684.1|	reuteri]
TBCP-1560_g_0612	gi|489770861|	glutamate/gamma-aminobutyrate family transporter	99.8	501	1	0	1	1503	1	501	0	945
	ref|WP_003674771.1|	YjeM [Lactobacillus reuteri]
TBCP-1560_g_0613	gi|489770858|	hypothetical protein [Lactobacillus reuteri]	100	296	0	0	1	888	1	296	0	571
	ref|WP_003674768.1|
TBCP-1560_g_0617	gi|489770854|	exopolysaccharide biosynthesis protein [Lactobacillus	99.216	255	2	0	1	765	1	255	0	498
	ref|WP_003674764.1|	reuteri]
TBCP-1560_g_0621	gi|489770847|	hypothetical protein [Lactobacillus reuteri]	100	473	0	0	1	1419	1	473	0	915
	ref|WP_003674757.1|
TBCP-1560_g_0623	gi|489770844|	hypothetical protein [Lactobacillus reuteri]	100	343	0	0	1	1029	1	343	0	699
	ref|WP_003674754.1|
TBCP-1560_g_0625	gi|953264848|	hypothetical protein predicted by prodigal	100	41	0	0	1	123	1	41	4.54E−22	77.8
	emb|CUU12186.1|	[Lactobacillus reuteri ATCC 53608]
TBCP-1560_g_0634	gi|489770828|	hypothetical protein [Lactobacillus reuteri]	100	190	0	0	1	570	1	190	8.04E−70	209
	ref|WP_003674738.1|
TBCP-1560_g_0638	gi|737169510|	hypothetical protein [Lactobacillus reuteri]	84.314	51	8	0	1	153	351	401	4.85E−24	90.9
	ref|WP_035155744.1|
TBCP-1560_g_0642	gi|526245865|	AI-2E family transporter [Lactobacillus reuteri]	99.731	372	1	0	1	1116	1	372	0	580
	ref|WP_020843219.1|
TBCP-1560_g_0644	gi|489770813|	glycosyl transferase family 2 [Lactobacillus	100	239	0	0	1	717	1	239	1.92E−163	450
	ref|WP_003674724.1|	reuteri]
TBCP-1560_g_0658	gi|1017201776|	hypothetical protein [Lactobacillus reuteri]	96.889	225	7	0	1	675	1	225	3.13E−113	322
	ref|WP_063164449.1|
TBCP-1560_g_0663	gi|489770795|	hypothetical protein [Lactobacillus reuteri]	100	61	0	0	1	183	1	61	5.63E−41	126
	ref|WP_003674706.1|
TBCP-1560_g_0664	gi|489760395|	phosphocarrier protein HPr [Lactobacillus	100	88	0	0	1	264	1	88	2.79E−60	177
	ref|WP_003664342.1|	reuteri]
TBCP-1560_g_0667	gi|512738944|	glycosyl transferase [Lactobacillus reuteri]	99.708	342	1	0	1	1026	1	342	0	711
	ref|WP_016496666.1|
TBCP-1560_g_0669	gi|489760389|	hypothetical protein [Lactobacillus reuteri]	98.78	82	1	0	1	246	1	82	3.49E−55	164
	ref|WP_003664336.1|
TBCP-1560_g_0670	gi|970368806|	FIG00742798: hypothetical protein [Lactobacillus	98.913	184	2	0	1	552	1	184	1.61E−138	383
	emb|CUR38328.1|	reuteri]
TBCP-1560_g_0674	gi|512738946|	anaerobic ribonucleoside triphosphate reductase	99.866	744	1	0	1	2232	1	744	0	1570
	ref|WP_016496668.1|	[Lactobacillus reuteri]
TBCP-1560_g_0675	gi|489766847|	anaerobic ribonucleoside-triphosphate reductase	99.479	192	1	0	1	576	1	192	1.08E−144	399
	ref|WP_003670774.1|	activating protein [Lactobacillus reuteri]
TBCP-1560_g_0676	gi|489771954|	hypothetical protein [Lactobacillus reuteri]	100	296	0	0	1	888	1	296	0	615
	ref|WP_003675859.1|
TBCP-1560_g_0678	gi|489771950|	phosphoenolpyruvate carboxykinase [Lactobacillus	100	566	0	0	1	1698	1	566	0	1144
	ref|WP_003675855.1|	reuteri]
TBCP-1560_g_0682	gi|489760280|	dihydropyrimidine dehydrogenase subunit	100	432	0	0	1	1296	1	432	0	900
	ref|WP_003664227.1|	B [Lactobacillus reuteri]
TBCP-1560_g_0686	gi|1017201782|	hypothetical protein [Lactobacillus reuteri]	100	147	0	0	1	441	1	147	1.53E−109	306
	ref|WP_063164455.1|
TBCP-1560_g_0693	gi|489771933|	3-methyladenine DNA glycosylase [Lactobacillus	100	179	0	0	1	537	1	179	2.08E−136	377
	ref|WP_003675838.1|	reuteri]
TBCP-1560_g_0697	gi|512738953|	MOP superfamily multidrug/oligosaccharidyl-	100	549	0	0	1	1647	1	549	0	1082
	ref|WP_016496675.1|	lipid/poly saccharide flippase transporter
		[Lactobacillus reuteri]
TBCP-1560_g_0699	gi|970363487|	Acetylornithine deacetylase/Succinyl-diaminopimelate	100	463	0	0	1	1389	5	467	0	949
	emb|CUR37516.1|	desuccinylase and related deacylases
		[Lactobacillus reuteri]
TBCP-1560_g_0703	gi|489771917|	hypothetical protein [Lactobacillus reuteri]	100	202	0	0	1	606	1	202	2.15E−152	419
	ref|WP_003675822.1|
TBCP-1560_g_0704	gi|489771915|	LacI family transcriptional regulator [Lactobacillus	100	338	0	0	1	1014	1	338	0	694
	ref|WP_003675820.1|	reuteri]
TBCP-1560_g_0706	gi|489764600|	hypothetical protein [Lactobacillus reuteri]	100	81	0	0	1	243	1	81	4.84E−54	161
	ref|WP_003668533.1|
TBCP-1560_g_0707	gi|489771912|	abortive infection protein [Lactobacillus	100	225	0	0	1	675	1	225	1.39E−155	429
	ref|WP_003675817.1|	reuteri]
TBCP-1560_g_0708	gi|489760247|	hypothetical protein [Lactobacillus reuteri]	100	92	0	0	1	276	1	92	3.18E−65	190
	ref|WP_003664194.1|
TBCP-1560_g_0710	gi|489771901|	2-amino-4-hydroxy-6-hydroxymethyldihydropteridine	100	170	0	0	1	510	1	170	3.41E−127	353
	ref|WP_003675807.1|	pyrophosphokinase [Lactobacillus
		reuteri]
TBCP-1560_g_0711	gi|512738959|	GTP cyclohydrolase I FolE [Lactobacillus	100	192	0	0	1	576	1	192	2.48E−145	400
	ref|WP_016496681.1|	reuteri]
TBCP-1560_g_0712	gi|489771897|	bifunctional folylpolyglutamate synthase/dihydrofolate	99.761	419	1	0	1	1257	1	419	0	828
	ref|WP_003675803.1|	synthase [Lactobacillus reuteri]
TBCP-1560_g_0713	gi|1017201785|	non-canonical purine NTP pyrophosphatase	100	195	0	0	1	585	1	195	1.39E−145	401
	ref|WP_063164458.1|	[Lactobacillus reuteri]
TBCP-1560_g_0716	gi|970368862|	FIG00745914: hypothetical protein [Lactobacillus	96.667	90	3	0	1	270	1	90	1.02E−48	148
	emb|CUR38275.1|	reuteri]
TBCP-1560_g_0724	gi|737170764|	peptidylprolyl isomerase [Lactobacillus reuteri]	99.658	292	1	0	1	876	1	292	0	553
	ref|WP_035156980.1|
TBCP-1560_g_0727	gi|489766912|	multidrug ABC transporter ATP-binding	100	245	0	0	1	735	1	245	0	502
	ref|WP_003670839.1|	protein [Lactobacillus reuteri]
TBCP-1560_g_0729	gi|489760201|	tRNA (guanosine(46)-N7)-methyltransferase	99.531	213	1	0	1	639	1	213	1.20E−146	405
	ref|WP_003664148.1|	TrmB [Lactobacillus reuteri]
TBCP-1560_g_0731	gi|512155633|	YSIRK Gram-positive signal peptide [Lactobacillus	100	97	0	0	1	291	1	97	9.51E−54	161
	gb|AGN98916.1|	reuteri I5007]
TBCP-1560_g_0732	gi|512155634|	LPXTG-motif cell wall anchor domain protein	99.038	104	1	0	1	312	1	104	6.66E−75	218
	gb|AGN98917.1|	[Lactobacillus reuteri I5007]
TBCP-1560_g_0732	gi|512155634|	LPXTG-motif cell wall anchor domain protein	100	20	0	0	303	362	102	121	9.85E−07	44.3
	gb|AGN98917.1|	[Lactobacillus reuteri I5007]
TBCP-1560_g_0733	gi|337728871|	hypothetical protein LRATCC53608_1237	99.877	811	1	0	1	2433	1	811	0	1516
	emb|CCC03990.1|	[Lactobacillus reuteri ATCC 53608]
TBCP-1560_g_0740	gi|489771859|	tRNA-binding protein [Lactobacillus reuteri]	100	215	0	0	1	645	1	215	2.70E−159	438
	ref|WP_003675765.1|
TBCP-1560_g_0742	gi|489771857|	prevent-host-death family protein [Lactobacillus	100	97	0	0	1	291	1	97	1.50E−67	196
	ref|WP_003675763.1|	reuteri]
TBCP-1560_g_0746	gi|1017201790|	DNA polymerase I [Lactobacillus reuteri]	99.887	888	1	0	1	2664	1	888	0	1781
	ref|WP_063164463.1|
TBCP-1560_g_0747	gi|489771849|	DNA-formamidopyrimidine glycosylase	100	276	0	0	1	828	1	276	0	574
	ref|WP_003675755.1|	[Lactobacillus reuteri]
TBCP-1560_g_0748	gi|489771847|	dephospho-CoA kinase [Lactobacillus reuteri]	100	199	0	0	1	597	1	199	5.28E−146	403
	ref|WP_003675753.1|
TBCP-1560_g_0749	gi|489764556|	NrdR family transcriptional regulator [Lactobacillus	98.701	154	2	0	1	462	1	154	9.09E−112	313
	ref|WP_003668489.1|	reuteri]
TBCP-1560_g_0751	gi|489771843|	primosomal protein Dnal [Lactobacillus reuteri]	99.681	313	1	0	1	939	1	313	0	644
	ref|WP_003675749.1|
TBCP-1560_g_0752	gi|489760160|	translation initiation factor IF-3 [Lactobacillus	100	170	0	0	1	510	1	170	1.23E−111	313
	ref|WP_003664107.1|	reuteri]
TBCP-1560_g_0754	gi|489771841|	50S ribosomal protein L20 [Lactobacillus	100	117	0	0	1	351	1	117	6.34E−81	232
	ref|WP_003675747.1|	reuteri]
TBCP-1560_g_0755	gi|489771839|	haloacid dehalogenase [Lactobacillus reuteri]	99.432	176	1	0	1	528	1	176	1.67E−127	354
	ref|WP_003675745.1|
TBCP-1560_g_0757	gi|489760152|	RNA-binding protein [Lactobacillus reuteri]	100	103	0	0	1	309	1	103	1.50E−70	204
	ref|WP_003664099.1|
TBCP-1560_g_0758	gi|489771836|	nicotinate-nicotinamide nucleotide adenyl	99.533	214	1	0	1	642	1	214	1.94E−161	443
	ref|WP_003675742.1|	yltransferase [Lactobacillus reuteri]
TBCP-1560_g_0759	gi|489771834|	HD domain-containing protein [Lactobacillus	99.51	204	1	0	1	612	1	204	1.63E−151	417
	ref|WP_003675740.1|	reuteri]
TBCP-1560_g_0761	gi|489771832|	SAM-dependent methyltransferase [Lactobacillus	100	246	0	0	1	738	1	246	0	516
	ref|WP_003675738.1|	reuteri]
TBCP-1560_g_0762	gi|337728844|	conserved hypothetical protein [Lactobacillus	100	377	0	0	1	1131	1	385	0	732
	emb|CCC03963.1|	reuteri ATCC 53608]
TBCP-1560_g_0763	gi|489760146|	DNA-binding protein [Lactobacillus reuteri]	99.459	185	1	0	1	555	1	185	6.15E−123	343
	ref|WP_003664093.1|
TBCP-1560_g_0764	gi|489760145|	50S ribosomal protein L32 [Lactobacillus	100	59	0	0	1	177	1	59	6.08E−30	99
	ref|WP_003664092.1|	reuteri]
TBCP-1560_g_0767	gi|489771827|	two-component sensor histidine kinase [Lactobacillus	100	491	0	0	1	1473	1	491	0	946
	ref|WP_003675733.1|	reuteri]
TBCP-1560_g_0768	gi|489771825|	glutamate/gamma-aminobutyrate family transporter	99.799	497	1	0	1	1491	1	497	0	941
	ref|WP_003675731.1|	YjeM [Lactobacillus reuteri]
TBCP-1560_g_0774	gi|489764526|	hydrolase [Lactobacillus reuteri]	100	168	0	0	1	504	1	168	3.12E−112	315
	ref|WP_003668459.1|
TBCP-1560_g_0775	gi|489771813|	HxlR family transcriptional regulator [Lactobacillus	100	112	0	0	1	336	1	112	7.83E−81	231
	ref|WP_003675719.1|	reuteri]
TBCP-1560_g_0777	gi|512738987|	phenylalanine-tRNA ligase subunit beta	100	805	0	0	1	2415	1	805	0	1626
	ref|WP_016496708.1|	[Lactobacillus reuteri]
TBCP-1560_g_0779	gi|489764516|	uridine kinase [Lactobacillus reuteri]	100	218	0	0	1	654	1	218	9.30E−115	325
	ref|WP_003668449.1|
TBCP-1560_g_0782	gi|489771804|	heme biosynthesis protein HemY [Lactobacillus	100	129	0	0	1	387	1	129	1.27E−93	265
	ref|WP_003675710.1|	reuteri]
TBCP-1560_g_0785	gi|489760102|	MULTISPECIES: 50 S ribosomal protein	100	49	0	0	1	147	1	49	5.01E−31	100
	ref|WP_003664049.1|	L33 [Lactobacillus]
TBCP-1560_g_0786	gi|489771798|	5-formyltetrahydrofolate cyclo-ligase [Lactobacillus	100	184	0	0	1	552	1	184	1.27E−137	380
	ref|WP_003675704.1|	reuteri]
TBCP-1560_g_0789	gi|489764508|	hypothetical protein [Lactobacillus reuteri]	100	79	0	0	1	237	1	79	1.19E−53	160
	ref|WP_003668441.1|
TBCP-1560_g_0793	gi|512738993|	tRNA (adenosine(37)-N6)-dimethylallyltransferase	100	307	0	0	1	921	1	307	0	635
	ref|WP_016496714.1|	MiaA [Lactobacillus reuteri]
TBCP-1560_g_0794	gi|489771787|	type I glutamate--ammonia ligase [Lactobacillus	100	447	0	0	1	1341	1	447	0	934
	ref|WP_003675693.1|	reuteri]
TBCP-1560_g_0797	gi|194453641|	conserved hypothetical protein [Lactobacillus	100	41	0	0	1	123	1	41	1.43E−22	79
	gb|EDX42538.1|	reuteri 100-23]
TBCP-1560_g_0798	gi|512738995|	lipoprotein [Lactobacillus reuteri]	99.647	283	1	0	1	849	1	283	0	531
	ref|WP_016496716.1|
TBCP-1560_g_0799	gi|489771780|	peptide-methionine (S)-S-oxide reductase	100	185	0	0	1	555	1	185	2.30E−140	387
	ref|WP_003675686.1|	[Lactobacillus reuteri]
TBCP-1560_g_0802	gi|512738998|	hypothetical protein [Lactobacillus reuteri]	100	313	0	0	1	939	1	313	0	638
	ref|WP_016496719.1|
TBCP-1560_g_0803	gi|489771772|	hypothetical protein [Lactobacillus reuteri]	100	168	0	0	1	504	1	168	1.26E−122	341
	ref|WP_003675678.1|
TBCP-1560_g_0806	gi|489771771|	TetR family transcriptional regulator [Lactobacillus	98.039	204	4	0	1	612	1	204	3.44E−148	409
	ref|WP_003675677.1|	reuteri]
TBCP-1560_g_0808	gi|489760062|	50S ribosomal protein L21 [Lactobacillus	100	102	0	0	1	306	1	102	5.16E−57	170
	ref|WP_003664009.1|	reuteri]
TBCP-1560_g_0810	gi|489760059|	50S ribosomal protein L27 [Lactobacillus	100	93	0	0	1	279	1	93	1.32E−64	189
	ref|WP_003664006.1|	reuteri]
TBCP-1560_g_0816	gi|489771761|	bifunctional 5,10-methylene-tetrahydrofolate	99.65	286	1	0	1	858	1	286	0	520
	ref|WP_003675667.1|	dehydrogenase/5,10-methylene-tetrahydrofolate
		cyclohydrolase [Lactobacillus reuteri]
TBCP-1560_g_0819	gi|489771755|	farnesyl-diphosphate synthase [Lactobacillus	100	290	0	0	1	870	1	290	0	556
	ref|WP_003675661.1|	reuteri]
TBCP-1560_g_0820	gi|489764469|	cell division protein FtsJ [Lactobacillus reuteri]	100	273	0	0	1	819	1	273	0	554
	ref|WP_003668402.1|
TBCP-1560_g_0821	gi|489771753|	ArgR family transcriptional regulator [Lactobacillus	100	150	0	0	1	450	1	150	1.48E−111	312
	ref|WP_003675659.1|	reuteri]
TBCP-1560_g_0822	gi|489771751|	DNA repair protein RecN [Lactobacillus	99.821	559	1	0	1	1677	1	559	0	1071
	ref|WP_003675657.1|	reuteri]
TBCP-1560_g_0826	gi|512739002|	pantothenate metabolism flavoprotein [Lactobacillus	99.751	401	1	0	1	1203	1	401	0	786
	ref|WP_016496722.1|	reuteri]
TBCP-1560_g_0829	gi|512739004|	16S rRNA (cytosine(967)-C(5))-methyltransferase	99.555	449	2	0	1	1347	1	449	0	886
	ref|WP_016496724.1|	[Lactobacillus reuteri]
TBCP-1560_g_0830	gi|948620368|	protein phosphatase 2C-like protein [Lactobacillus	98.467	261	4	0	1	783	1	261	0	527
	gb|KRK52332.1|	reuteri DSM 20016]
TBCP-1560_g_0831	gi|1017201882|	protein kinase [Lactobacillus reuteri]	100	634	0	0	1	1902	1	634	0	1305
	ref|WP_063164555.1|
TBCP-1560_g_0832	gi|489771738|	ribosome small subunit-dependent GTPase	99.662	296	1	0	1	888	1	296	0	613
	ref|WP_003675644.1|	A [Lactobacillus reuteri]
TBCP-1560_g_0833	gi|227071790|	ribulose-phosphate 3-epimerase [Lactobacillus	100	217	0	0	1	651	15	231	7.04E−159	437
	gb|EEI10079.1|	reuteri MM2-3]
TBCP-1560_g_0835	gi|948620363|	hypothetical protein FC53_GL000029 [Lactobacillus	100	61	0	0	1	183	22	82	8.69E−40	124
	gb|KRK52327.1|	reuteri DSM 20016]
TBCP-1560_g_0836	gi|227185635|	hypothetical protein HMPREF0534_0958	100	120	0	0	1	360	3	122	2.50E−82	236
	gb|EEI65706.1|	[Lactobacillus reuteri CF48-3A]
TBCP-1560_g_0839	gi|1017201886|	phosphate acyltransferase [Lactobacillus reuteri]	100	343	0	0	1	1029	1	343	0	694
	ref|WP_063164559.1|
TBCP-1560_g_0844	gi|489759870|	DNA-binding protein [Lactobacillus reuteri]	100	113	0	0	1	339	1	113	1.34E−79	228
	ref|WP_003663818.1|
TBCP-1560_g_0846	gi|489759868|	30S ribosomal protein S16 [Lactobacillus	98.901	91	1	0	1	273	1	91	3.03E−64	187
	ref|WP_003663816.1|	reuteri]
TBCP-1560_g_0847	gi|489771725|	RNA-binding protein [Lactobacillus reuteri]	100	86	0	0	1	258	1	86	1.89E−58	172
	ref|WP_003675631.1|
TBCP-1560_g_0848	gi|970363287|	16S rRNA processing protein RimM [Lactobacillus	100	168	0	0	1	504	1	168	5.18E−110	309
	emb|CUR37646.1|	reuteri]
TBCP-1560_g_0851	gi|148531407|	LSU ribosomal protein L19P [Lactobacillus	100	128	0	0	1	384	1	128	5.23E−90	256
	gb|ABQ83406.1|	reuteri DSM 20016]
TBCP-1560_g_0853	gi|337728756|	hypothetical protein LRATCC53608_1122	100	110	0	0	1	330	2	111	1.99E−79	227
	emb|CCC03875.1|	[Lactobacillus reuteri ATCC 53608]
TBCP-1560_g_0854	gi|489771712|	exodeoxyribonuclease [Lactobacillus reuteri]	99.636	275	1	0	1	825	1	275	0	573
	ref|WP_003675618.1|
TBCP-1560_g_0857	gi|518083618|	subtype II CRISPR-associated endonuclease	97.682	302	7	0	1	906	1	302	0	610
	ref|WP_019253826.1|	Cas1 [Lactobacillus reuteri]
TBCP-1560_g_0858	gi|518083617|	CRISPR-associated endonuclease Cas2 [Lactobacillus	100	101	0	0	1	303	1	101	1.28E−71	207
	ref|WP_019253825.1|	reuteri]
TBCP-1560_g_0859	gi|518083616|	type II-A CRISPR-associated protein Csn2	98.206	223	4	0	1	669	1	223	2.39E−163	449
	ref|WP_019253824.1|	[Lactobacillus reuteri]
TBCP-1560_g_0861	gi|489763052|	PTS sugar transporter subunit IIA [Lactobacillus	99.507	203	1	0	1	609	1	203	4.87E−121	357
	ref|WP_003666989.1|	reuteri]
TBCP-1560_g_0862	gi|737166902|	phosphotransferase enzyme IIA component	96.078	408	16	0	1	1224	233	640	0	768
	ref|WP_035153222.1|	[Lactobacillus reuteri]
TBCP-1560_g_0863	gi|970370498|	Beta-galactosidase [Lactobacillus reuteri]	97.173	672	19	0	1	2016	1	672	0	1370
	emb|CUR40930.1|
TBCP-1560_g_0865	gi|512739014|	1,4-dihydroxy-2-naphthoate polyprenyltransferase	100	299	0	0	1	897	1	299	0	565
	ref|WP_016496734.1|	[Lactobacillus reuteri]
TBCP-1560_g_0868	gi|489771699|	metallophosphoesterase [Lactobacillus reuteri]	100	279	0	0	40	876	14	292	0	583
	ref|WP_003675605.1|
TBCP-1560_g_0869	gi|489763069|	30S ribosomal protein S14 [Lactobacillus	100	89	0	0	1	267	1	89	2.04E−61	180
	ref|WP_003667006.1|	reuteri]
TBCP-1560_g_0871	gi|489761765|	hypothetical protein [Lactobacillus reuteri]	100	18	0	0	172	225	213	230	1.92E−06	42.4
	ref|WP_003665706.1|
TBCP-1560_g_0872	gi|970364325|	L-serine dehydratase, beta subunit [Lactobacillus	99.091	220	2	0	1	660	1	220	1.03E−162	447
	emb|CUR36723.1|	reuteri]
TBCP-1560_g_0873	gi|489771691|	L-serine dehydratase, iron-sulfur-dependent	100	293	0	0	1	879	1	293	1.60E−174	483
	ref|WP_003675597.1|	subunit alpha [Lactobacillus reuteri]
TBCP-1560_g_0874	gi|489771689|	peroxiredoxin [Lactobacillus reuteri]	99.465	187	1	0	1	561	1	187	3.64E−140	387
	ref|WP_003675595.1|
TBCP-1560_g_0876	gi|512155769|	LPXTG-motif cell wall anchor domain protein	99.719	356	1	0	1	1068	1	356	0	632
	gb|AGN99052.1|	[Lactobacillus reuteri I5007]
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	42.133	966	535	3	472	3363	139	1082	7.25E−151	502
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	42.65	966	550	2	472	3363	539	1502	6.86E−150	499
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	41.362	984	537	3	472	3363	49	1012	4.45E−149	496
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	41.667	984	534	4	472	3363	69	1032	6.36E−149	496
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	41.786	974	537	3	472	3363	509	1462	8.84E−149	496
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	42.17	977	525	4	472	3342	1429	2385	1.24E−148	495
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	42.402	974	527	5	472	3357	1159	2110	1.71E−148	495
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	41.814	959	534	3	472	3342	419	1355	2.68E−148	494
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	42.547	966	541	3	472	3363	1699	2652	4.67E−148	494
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	41.159	984	539	3	472	3363	349	1312	6.86E−148	493
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	41.615	966	540	3	472	3363	239	1182	2.26E−147	491
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	41.684	974	538	3	472	3363	1369	2322	1.11E−146	489
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	42.641	992	539	4	472	3357	1009	2000	1.63E−146	489
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	41.786	974	543	3	472	3357	259	1220	1.70E−146	489
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	42.284	972	541	3	472	3357	849	1810	1.84E−146	489
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	41.018	1002	525	4	472	3363	1539	2512	2.50E−146	488
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	41.519	1014	533	7	472	3363	1559	2562	2.75E−146	488
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	42.427	964	541	3	472	3357	1269	2220	3.72E−146	488
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	42.029	966	536	3	472	3363	829	1772	4.08E−146	488
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	41.273	974	532	4	472	3363	2279	3222	4.44E−146	488
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	41.718	966	539	3	472	3363	989	1932	7.66E−146	487
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	42.186	979	542	2	472	3342	1819	2795	1.46E−145	486
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	41.822	966	538	3	472	3363	719	1662	1.56E−145	486
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	41.383	969	534	4	472	3342	679	1625	2.27E−145	486
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	41.889	974	546	2	472	3363	2129	3092	3.28E−145	485
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	41.833	1004	534	4	472	3363	1289	2282	3.50E−145	485
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	41.273	974	538	3	472	3357	19	970	8.00E−145	484
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	41.65	982	543	3	472	3357	1209	2180	9.04E−145	484
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	41.925	966	537	3	472	3363	1189	2132	1.41E−144	483
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	41.955	982	540	4	472	3357	1769	2740	1.78E−144	483
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	41.159	984	549	3	472	3363	649	1622	3.52E−144	482
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	40.744	994	539	4	472	3363	2079	3052	1.29E−143	481
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	41.803	976	534	5	472	3363	1879	2832	1.72E−143	480
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	40.895	961	534	3	511	3357	2	940	5.58E−142	476
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	41.147	994	535	6	472	3363	2149	3122	7.97E−142	475
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	40.335	1014	535	8	472	3363	2169	3162	2.26E−140	471
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	42.105	969	547	4	472	3342	1969	2935	6.28E−140	469
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	41.922	947	520	4	472	3252	2299	3235	1.19E−139	469
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	41.287	901	489	2	781	3363	2	902	6.78E−136	457
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	23.209	1103	749	9	203	3397	2026	3068	2.67E−66	246
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	23.727	923	636	8	761	3397	1960	2858	6.35E−66	245
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	23.911	941	652	14	761	3397	650	1588	1.25E−65	244
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	23.877	913	647	10	761	3397	1090	1988	5.28E−65	242
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	23.663	879	631	3	761	3397	1880	2718	9.74E−65	241
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	23.793	891	635	7	761	3397	200	1058	3.06E−64	239
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	42.126	508	284	1	472	1965	2729	3236	5.92E−64	239
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	23.749	939	636	14	761	3397	770	1688	1.14E−63	238
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	23.663	879	623	7	761	3355	2020	2864	1.42E−63	238
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	23.119	1103	710	17	203	3397	1646	2648	1.96E−63	237
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	23.619	923	647	11	761	3397	90	998	2.71E−63	236
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	23.161	1101	742	15	203	3397	1196	2228	8.72E−63	235
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	23.106	1069	732	10	203	3397	816	1798	1.54E−62	234
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	23.818	1079	762	12	203	3397	1466	2498	4.48E−62	233
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	23.432	909	666	5	761	3397	20	928	6.24E−62	232
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	22.477	1090	757	9	212	3397	319	1348	3.68E−61	230
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	22.341	1119	749	16	203	3397	456	1508	6.58E−61	229
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	22.53	1012	686	11	494	3397	1371	2328	1.93E−60	228
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	22.955	880	634	6	884	3397	1	878	3.91E−60	226
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	23.616	885	606	9	761	3355	1010	1844	1.26E−59	225
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	22.533	1074	710	11	266	3397	1157	2138	1.50E−59	224
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	23.367	903	634	10	761	3397	1150	2018	3.96E−59	223
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	21.875	1024	696	10	482	3397	647	1618	2.26E−58	221
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	22.857	980	682	7	494	3397	201	1118	3.39E−58	220
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	22.574	979	658	16	761	3397	270	1248	1.01E−57	219
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	19.671	1154	813	32	3402	199	2057	3182	6.99E−52	200
	ref|WP_050764451.1|
TBCP-1560_g_0881	gi|915327763|	hypothetical protein [Lactobacillus reuteri]	21.024	1113	807	25	3399	181	928	2008	1.24E−49	192
	ref|WP_050764451.1|
TBCP-1560_g_0882	gi|917061910|	hypothetical protein [Lactobacillus reuteri]	60	30	12	0	19	108	9	38	1.04E−06	42.7
	ref|WP_051668622.1|
TBCP-1560_g_0887	gi|489771675|	accessory Sec system protein Asp3 [Lactobacillus	100	295	0	0	1	885	1	295	0	602
	ref|WP_003675581.1|	reuteri]
TBCP-1560_g_0889	gi|489771671|	accessory Sec system glycosyltransferase	100	512	0	0	1	1536	1	512	0	1058
	ref|WP_003675577.1|	GtfA [Lactobacillus reuteri]
TBCP-1560_g_0891	gi|512739028|	hypothetical protein [Lactobacillus reuteri]	98.462	65	1	0	1	195	1	65	2.77E−17	67.4
	ref|WP_016496748.1|
TBCP-1560_g_0892	gi|1017201897|	adhesin [Lactobacillus reuteri]	99.065	107	1	0	1	321	1	107	1.29E−58	180
	ref|WP_063164570.1|
TBCP-1560_g_0893	gi|1017201898|	cell wall protein [Lactobacillus reuteri]	100	230	0	0	61	750	21	250	2.63E−141	420
	ref|WP_063164571.1|
TBCP-1560_g_0893	gi|1017201898|	cell wall protein [Lactobacillus reuteri]	99.462	186	1	0	1174	1731	382	567	1.43E−21	97.8
	ref|WP_063164571.1|
TBCP-1560_g_0894	gi|512155787|	IstB ATP binding domain-containing protein	99.519	208	1	0	1	624	1	208	2.11E−155	427
	gb|AGN99070.1|	[Lactobacillus reuteri I5007]
TBCP-1560_g_0897	gi|489772852|	potassium transporter [Lactobacillus reuteri]	100	609	0	0	1	1827	1	609	0	1095
	ref|WP_003676754.1|
TBCP-1560_g_0898	gi|489759560|	4-oxalocrotonate tautomerase [Lactobacillus	100	61	0	0	1	183	1	61	1.26E−39	123
	ref|WP_003663508.1|	reuteri]
TBCP-1560_g_0899	gi|970365238|	Glycerol kinase [Lactobacillus reuteri]	98.8	500	6	0	1	1500	1	500	0	948
	emb|CUR43848.1|
TBCP-1560_g_0900	gi|970364616|	FIG00745619: hypothetical protein [Lactobacillus	100	167	0	0	1	501	1	167	5.64E−124	345
	emb|CUR36442.1|	reuteri]
TBCP-1560_g_0903	gi|489772841|	exopolysaccharide biosynthesis protein [Lactobacillus	98.969	291	3	0	1	873	1	291	0	592
	ref|WP_003676743.1|	reuteri]
TBCP-1560_g_0904	gi|489772839|	exopolysaccharide biosynthesis protein [Lactobacillus	98.712	233	3	0	1	699	1	233	1.06E−169	467
	ref|WP_003676741.1|	reuteri]
TBCP-1560_g_0905	gi|970369186|	Lipid carrier: UDP-N-acetylgalactosaminyltransferase	68.72	211	64	1	10	642	5	213	2.66E−108	309
	emb|CUR42563.1|	[Lactobacillus reuteri]
TBCP-1560_g_0906	gi|659902175|	glycosyl transferase [Lactobacillus reuteri]	63.947	380	137	0	4	1143	9	388	5.68E−172	484
	gb|KEK16947.1|
TBCP-1560_g_0907	gi|526245771|	hypothetical protein [Lactobacillus reuteri]	25.714	420	266	13	10	1218	3	393	1.57E−36	137
	ref|WP_020843125.1|
TBCP-1560_g_0909	gi|970369097|	capsular polysaccharide biosynthesis protein	30.952	294	190	8	136	999	57	343	2.99E−24	102
	emb|CUR38065.1|	[Lactobacillus reuteri]
TBCP-1560_g_0915	gi|489772294|	ATPase AAA [Lactobacillus reuteri]	100	245	0	0	1	735	1	245	0	506
	ref|WP_003676198.1|
TBCP-1560_g_0918	gi|1017201766|	dTDP-glucose 4,6-dehydratase [Lactobacillus	99.711	346	1	0	1	1038	1	346	0	671
	ref|WP_063164439.1|	reuteri]
TBCP-1560_g_0924	gi|1017201899|	integrase [Lactobacillus reuteri]	97.778	315	7	0	10	954	66	380	0	644
	ref|WP_063164572.1|
TBCP-1560_g_0925	gi|953265103|	Insertion sequence IS232 putative ATP-binding	99.592	245	1	0	1	735	1	245	0	503
	emb|CUU12464.1|	protein [Lactobacillus reuteri ATCC
		53608]
TBCP-1560_g_0926	gi|1017201453|	hypothetical protein [Lactobacillus reuteri]	31.311	412	192	16	31	1137	5	368	3.68E−39	144
	ref|WP_063164126.1|
TBCP-1560_g_0927	gi|518082660|	hypothetical protein [Lactobacillus reuteri]	34.286	105	63	3	640	942	184	286	8.05E−07	51.2
	ref|WP_019252868.1|
TBCP-1560_g_0932	gi|970367365|	hypothetical protein LRLP16767_LR3C6_01581	90.244	82	8	0	1	246	1	82	6.22E−49	155
	emb|CUR39614.1|	[Lactobacillus reuteri]
TBCP-1560_g_0934	gi|489768400|	homocysteine S-methyltransferase [Lactobacillus	97.742	310	7	0	1	930	1	310	0	594
	ref|WP_003672322.1|	reuteri]
TBCP-1560_g_0936	gi|489763124|	hypothetical protein [Lactobacillus reuteri]	98.824	85	1	0	1	255	1	85	4.19E−55	164
	ref|WP_003667061.1|
TBCP-1560_g_0939	gi|970363280|	PemK family protein [Lactobacillus reuteri]	98.78	82	1	0	1	246	1	82	4.87E−57	169
	emb|CUR37717.1|
TBCP-1560_g_0940	gi|970363279|	FIG00751385: hypothetical protein [Lactobacillus	98.75	80	1	0	1	240	1	80	6.00E−54	160
	emb|CUR37716.1|	reuteri]
TBCP-1560_g_0941	gi|489772795|	hypothetical protein [Lactobacillus reuteri]	98.374	615	10	0	1	1845	1	615	0	1117
	ref|WP_003676697.1|
TBCP-1560_g_0942	gi|1017201365|	DEAD/DEAH box helicase [Lactobacillus	99.914	1161	1	0	1	3483	1	1161	0	2331
	ref|WP_063164038.1|	reuteri]
TBCP-1560_g_0945	gi|518081247|	exonuclease sbcCD subunit D [Lactobacillus	98.925	372	4	0	1	1116	1	372	0	763
	ref|WP_019251455.1|	reuteri]
TBCP-1560_g_0952	gi|489768426|	phosphoglycerate kinase [Lactobacillus reuteri]	98.561	278	4	0	1	834	1	278	0	544
	ref|WP_003672348.1|
TBCP-1560_g_0955	gi|657710404|	hypothetical protein [Lactobacillus reuteri]	96.875	192	6	0	1	576	144	335	9.14E−134	377
	ref|WP_029507401.1|
TBCP-1560_g_0966	gi|518083012|	hypothetical protein [Lactobacillus reuteri]	99.01	101	1	0	1	303	1	101	3.20E−69	201
	ref|WP_019253220.1|
TBCP-1560_g_0967	gi|489770069|	hypothetical protein [Lactobacillus reuteri]	97.946	633	13	0	1	1899	1	633	0	1228
	ref|WP_003673982.1|
TBCP-1560_g_0973	gi|754210193|	peptide ABC transporter substrate-binding	100	317	0	0	1	951	15	331	0	544
	ref|WP_041821667.1|	protein [Lactobacillus reuteri]
TBCP-1560_g_0978	gi|970363231|	FIG00742465: hypothetical protein [Lactobacillus	99.655	290	1	0	1	870	1	290	0	593
	emb|CUR37781.1|	reuteri]
TBCP-1560_g_0981	gi|489770084|	DNA-binding response regulator [Lactobacillus	100	217	0	0	1	651	1	217	7.49E−160	439
	ref|WP_003673997.1|	reuteri]
TBCP-1560_g_0983	gi|489763785|	hypothetical protein [Lactobacillus reuteri]	98.936	94	1	0	1	282	1	94	4.09E−51	154
	ref|WP_003667721.1|
TBCP-1560_g_0984	gi|512739103|	molybdopterin-guanine dinucleotide biosynthesis	98.947	190	2	0	1	570	1	190	4.66E−143	395
	ref|WP_016496821.1|	protein MobA [Lactobacillus reuteri]
TBCP-1560_g_0985	gi|489770088|	molybdopterin-guanine dinucleotide biosynthesis	99.383	162	1	0	1	486	1	162	1.55E−120	335
	ref|WP_003674001.1|	protein B [Lactobacillus reuteri]
TBCP-1560_g_0986	gi|512739104|	molybdopterin molybdenumtransferase MoeA	100	405	0	0	1	1215	1	405	0	839
	ref|WP_016496822.1|	[Lactobacillus reuteri]
TBCP-1560_g_0987	gi|512739105|	molybdenum cofactor biosynthesis protein	100	165	0	0	1	495	1	165	1.98E−109	308
	ref|WP_016496823.1|	[Lactobacillus reuteri]
TBCP-1560_g_0988	gi|512739106|	molybdopterin biosynthesis protein MoeB	99.41	339	1	1	1	1014	1	339	0	697
	ref|WP_016496824.1|	[Lactobacillus reuteri]
TBCP-1560_g_0989	gi|489770095|	nitrate reductase subunit alpha [Lactobacillus	99.918	1221	1	0	1	3663	1	1221	0	2561
	ref|WP_003674008.1|	reuteri]
TBCP-1560_g_0990	gi|489770097|	nitrate reductase subunit beta [Lactobacillus	99.615	519	2	0	1	1557	1	519	0	1087
	ref|WP_003674010.1|	reuteri]
TBCP-1560_g_0991	gi|489759699|	nitrate reductase molybdenum cofactor assembly	99.479	192	1	0	1	576	1	192	6.83E−138	382
	ref|WP_003663647.1|	chaperone [Lactobacillus reuteri]
TBCP-1560_g_0992	gi|489763803|	nitrate reductase subunit gamma [Lactobacillus	100	213	0	0	1	639	1	213	2.57E−157	434
	ref|WP_003667738.1|	reuteri]
TBCP-1560_g_0998	gi|948614637|	hypothetical protein FC53_GL001335 [Lactobacillus	98.246	57	1	0	1	171	1	57	1.67E−38	120
	gb|KRK46737.1|	reuteri DSM 20016]
TBCP-1560_g_1000	gi|489763814|	beta-hydroxyacyl-ACP dehydratase [Lactobacillus	100	145	0	0	1	435	4	148	2.35E−106	298
	ref|WP_003667749.1|	reuteri]
TBCP-1560_g_1002	gi|489770110|	3-oxoacyl-ACP synthase III [Lactobacillus	99.383	324	2	0	1	972	1	324	0	660
	ref|WP_003674023.1|	reuteri]
TBCP-1560_g_1003	gi|148531252|	phosphopantetheine-binding protein [Lactobacillus	100	82	0	0	1	246	1	82	2.24E−50	152
	gb|ABQ83251.11	reuteri DSM 20016]
TBCP-1560_g_1004	gi|489770114|	ACP S-malonyltransferase [Lactobacillus	100	316	0	0	1	948	1	316	0	656
	ref|WP_003674027.1|	reuteri]
TBCP-1560_g_1005	gi|489770116|	beta-ketoacyl-ACP reductase [Lactobacillus	100	244	0	0	1	732	1	244	0	497
	ref|WP_003674029.1|	reuteri]
TBCP-1560_g_1010	gi|489770124|	acetyl-CoA carboxylase subunit beta [Lactobacillus	100	289	0	0	1	867	1	289	0	605
	ref|WP_003674037.1|	reuteri]
TBCP-1560_g_1011	gi|489770126|	acetyl-CoA carboxylase carboxyl transferase	100	258	0	0	1	774	1	258	6.73E−171	471
	ref|WP_003674039.1|	subunit alpha [Lactobacillus reuteri]
TBCP-1560_g_1017	gi|868713964|	putative alpha/beta hydrolase [Lactobacillus	98.089	157	3	0	1	471	129	285	2.30E−111	317
	gb|AKP01217. 1|	reuteri]
TBCP-1560_g_1020	gi|489770141|	hypothetical protein [Lactobacillus reuteri]	100	93	0	0	82	360	28	120	2.43E−45	142
	ref|WP_003674054.1|
TBCP-1560_g_1033	gi|489763578|	two-component system response regulator	48.921	278	141	1	1	831	1	278	5.90E−99	290
	ref|WP_003667514.1|	[Lactobacillus reuteri]
TBCP-1560_g_1034	gi|659902062|	amino acid permease [Lactobacillus reuteri]	27.489	462	324	3	79	1464	23	473	1.64E−46	167
	gb|KEK16839.1|
TBCP-1560_g_1035	gi|148531853|	Transposase and inactivated derivatives IS30	98.75	160	2	0	1	480	1	160	4.94E−117	327
	gb|ABQ83852.1|	family-like protein [Lactobacillus reuteri
		DSM 20016]
TBCP-1560_g_1038	gi|970364069|	Phage protein [Lactobacillus reuteri]	99.76	417	1	0	1	1251	1	417	0	833
	emb|CUR37081.1|
TBCP-1560_g_1042	gi|970364066|	hypothetical protein LRLP16767_LRPG3B_00870	95.652	46	2	0	34	171	1	46	1.07E−26	90.1
	emb|CUR37078.1|	[Lactobacillus reuteri]
TBCP-1560_g_1043	gi|970364065|	hypothetical protein LRLP16767_LRPG3B_00869	98.857	175	2	0	1	525	1	175	7.26E−113	317
	emb|CUR37077.1|	[Lactobacillus reuteri]
TBCP-1560_g_1044	gi|970364064|	hypothetical protein LRLP16767_LRPG3B_00868	100	114	0	0	1	342	1	114	7.66E−83	236
	emb|CUR37076.1|	[Lactobacillus reuteri]
TBCP-1560_g_1045	gi|970364063|	Integrase [Lactobacillus reuteri]	99.728	367	1	0	1	1101	1	367	0	721
	emb|CUR37075.1|
TBCP-1560_g_1055	gi|489759851|	glyoxalase [Lactobacillus reuteri]	100	130	0	0	1	390	1	130	3.04E−95	269
	ref|WP_003663799.1|
TBCP-1560_g_1064	gi|489770177|	LysR family transcriptional regulator [Lactobacillus	100	294	0	0	1	882	1	294	0	602
	ref|WP_003674090.1|	reuteri]
TBCP-1560_g_1066	gi|489770178|	MFS transporter [Lactobacillus reuteri]	100	296	0	0	1	888	1	296	0	611
	ref|WP_003674091.1|
TBCP-1560_g_1067	gi|489762281|	hypothetical protein [Lactobacillus reuteri]	100	34	0	0	1	102	1	34	1.28E−16	64.7
	ref|WP_003666221.1|
TBCP-1560_g_1076	gi|489770192|	hypothetical protein [Lactobacillus reuteri]	100	560	0	0	1	1680	1	560	0	1070
	ref|WP_003674105.1|
TBCP-1560_g_1082	gi|489770201|	ribonuclease HI [Lactobacillus reuteri]	100	131	0	0	1	393	1	131	1.34E−81	234
	ref|WP_003674114.1|
TBCP-1560_g_1088	gi|489770207|	penicillin-binding protein 1A [Lactobacillus	100	676	0	0	1	2028	1	676	0	1342
	ref|WP_003674120.1|	reuteri]
TBCP-1560_g_1093	gi|489770214|	mevalonate kinase [Lactobacillus reuteri]	100	316	0	0	1	948	1	316	0	590
	ref|WP_003674127.1|
TBCP-1560_g_1094	gi|489770215|	diphosphomevalonate decarboxylase [Lactobacillus	100	323	0	0	1	969	1	323	0	535
	ref|WP_003674128.1|	reuteri]
TBCP-1560_g_1095	gi|489762243|	phosphomevalonate kinase [Lactobacillus	99.733	375	1	0	1	1125	1	375	0	728
	ref|WP_003666183.1|	reuteri]
TBCP-1560_g_1096	gi|489770216|	type 2 isopentenyl-diphosphate Delta-isomerase	100	348	0	0	1	1044	1	348	0	686
	ref|WP_003674129.1|	[Lactobacillus reuteri]
TBCP-1560_g_1099	gi|489770219|	LacI family transcriptional regulator [Lactobacillus	100	339	0	0	1	1017	1	339	0	698
	ref|WP_003674132.1|	reuteri]
TBCP-1560_g_1100	gi|489770220|	fructokinase [Lactobacillus reuteri]	99.658	292	1	0	1	876	1	292	0	579
	ref|WP_003674133.1|
TBCP-1560_g_1108	gi|194452845|	Cof-like hydrolase [Lactobacillus reuteri	100	272	0	0	1	816	1	272	0	560
	gb|EDX41743.1|	100-23]
TBCP-1560_g_1120	gi|489770238|	iron transporter FeoA [Lactobacillus reuteri]	100	67	0	0	1	201	1	67	1.31E−47	144
	ref|WP_003674151.1|
TBCP-1560_g_1121	gi|489770239|	ferrous iron transport protein B [Lactobacillus	100	663	0	0	1	1989	1	663	0	1322
	ref|WP_003674152.1|	reuteri]
TBCP-1560_g_1125	gi|489770243|	thioesterase [Lactobacillus reuteri]	100	124	0	0	1	372	1	124	1.99E−89	254
	ref|WP_003674156.1|
TBCP-1560_g_1128	gi|970365298|	FIG00744546: hypothetical protein [Lactobacillus	100	962	0	0	1	2886	1	962	0	1945
	emb|CUR43832.1|	reuteri]
TBCP-1560_g_1129	gi|970365299|	Potassium voltage-gated channel subfamily	99.17	241	2	0	1	723	1	241	4.53E−154	427
	emb|CUR43833.1|	KQT; possible potassium channel, VIC
		family [Lactobacillus reuteri]
TBCP-1560_g_1139	gi|659901251|	XRE family transcriptional regulator [Lactobacillus	91.139	79	7	0	1	237	1	79	1.42E−47	144
	gb|KEK16045.1|	reuteri]
TBCP-1560_g_1145	gi|970371392|	hypothetical protein LRLP16767_LR202_00134	100	197	0	0	1	591	1	197	1.83E−140	404
	emb|CUR40084.1|	[Lactobacillus reuteri]
TBCP-1560_g_1147	gi|489764038|	hypothetical protein [Lactobacillus reuteri]	99.387	163	1	0	1	489	1	163	3.94E−93	266
	ref|WP_003667972.1|
TBCP-1560_g_1150	gi|489770273|	hypothetical protein [Lactobacillus reuteri]	100	103	0	0	1	309	1	103	1.38E−46	144
	ref|WP_003674186.1|
TBCP-1560_g_1153	gi|970365843|	Gluconate permease, Bsu4004 homolog	98.913	276	3	0	55	882	19	294	0	544
	emb|CUR43297.1|	[Lactobacillus reuteri]
TBCP-1560_g_1154	gi|337727830|	gluconate transport protein [Lactobacillus	100	139	0	0	1	417	239	377	8.32E−71	216
	emb|CCC02917.1|	reuteri ATCC 53608]
TBCP-1560_g_1155	gi|915462619|	transcriptional regulator [Lactobacillus reuteri]	100	334	0	0	1	1002	6	339	0	688
	ref|WP_050802152.1|
TBCP-1560_g_1159	gi|489762152|	hypothetical protein [Lactobacillus reuteri]	100	63	0	0	1	189	1	63	8.23E−27	91.3
	ref|WP_003666092.1|
TBCP-1560_g_1170	gi|489762134|	glycerol-3-phosphate acyltransferase [Lactobacillus	100	209	0	0	1	627	1	209	5.19E−149	411
	ref|WP_003666074.1|	reuteri]
TBCP-1560_g_1180	gi|489762122|	hemolysin III [Lactobacillus reuteri]	100	214	0	0	1	642	1	214	5.90E−120	338
	ref|WP_003666062.1|
TBCP-1560_g_1203	gi|754210243|	integral membrane protein [Lactobacillus	100	81	0	0	1	243	1	81	9.49E−56	165
	ref|WP_041821711.1|	reuteri]
TBCP-1560_g_1240	gi|337727907|	conserved hypothetical protein [Lactobacillus	100	58	0	0	1	174	1	58	4.42E−37	116
	emb|CCC02996.1|	reuteri ATCC 53608]
TBCP-1560_g_1270	gi|227070166|	Acyltransferase [Lactobacillus reuteri MM2-	99.588	243	1	0	1	729	1	243	3.64E−179	490
	gb|EEI08541.1|	3]
TBCP-1560_g_1273	gi|489770423|	repressor LexA [Lactobacillus reuteri]	100	208	0	0	1	624	1	208	4.21E−156	429
	ref|WP_003674334.1|
TBCP-1560_g_1277	gi|489770427|	adenine phosphoribosyltransferase [Lactobacillus	100	172	0	0	1	516	1	172	8.74E−123	342
	ref|WP_003674338.1|	reuteri]
TBCP-1560_g_1293	gi|489762895|	30S ribosomal protein S20 [Lactobacillus	100	84	0	0	1	252	1	84	2.02E−54	162
	ref|WP_003666832.1|	reuteri]
TBCP-1560_g_1310	gi|489770468|	peptide deformylase [Lactobacillus reuteri]	100	186	0	0	1	558	1	186	7.87E−139	384
	ref|WP_003674379.1|
TBCP-1560_g_1312	gi|489762866|	hypothetical protein [Lactobacillus reuteri]	100	70	0	0	1	210	1	70	1.57E−47	144
	ref|WP_003666803.1|
TBCP-1560_g_1313	gi|489770470|	diacylglycerol kinase [Lactobacillus reuteri]	100	315	0	0	1	945	1	315	0	654
	ref|WP_003674381.1|
TBCP-1560_g_1320	gi|512739239|	sugar diacid utilization regulator [Lactobacillus	99.716	352	1	0	1	1056	1	352	0	673
	ref|WP_016496953.1|	reuteri]
TBCP-1560_g_1328	gi|489770493|	aspartate kinase [Lactobacillus reuteri]	100	452	0	0	1	1356	1	452	0	925
	ref|WP_003674404.1|
TBCP-1560_g_1381	gi|489762939|	hypothetical protein [Lactobacillus reuteri]	100	163	0	0	1	489	1	163	2.48E−120	335
	ref|WP_003666876.1|
TBCP-1560_g_1407	gi|489772128|	mechanosensitive ion channel protein [Lactobacillus	100	291	0	0	1	873	1	291	0	544
	ref|WP_003676032.1|	reuteri]
TBCP-1560_g_1455	gi|489762694|	30S ribosomal protein S4 [Lactobacillus	100	201	0	0	1	603	1	201	2.21E−149	412
	ref|WP_003666632.1|	reuteri]
TBCP-1560_g_1462	gi|489762674|	hypothetical protein [Lactobacillus reuteri]	100	247	0	0	1	741	1	247	0	502
	ref|WP_003666612.1|
TBCP-1560_g_1466	gi|489772205|	recombinase RarA [Lactobacillus reuteri]	100	434	0	0	1	1302	1	434	0	861
	ref|WP_003676109.1|
TBCP-1560_g_1467	gi|489762666|	hypothetical protein [Lactobacillus reuteri]	100	86	0	0	37	294	13	98	2.71E−59	176
	ref|WP_003666604.1|
TBCP-1560_g_1469	gi|489772208|	GntR family transcriptional regulator [Lactobacillus	99.178	365	3	0	1	1095	1	365	0	750
	ref|WP_003676112.1|	reuteri]
TBCP-1560_g_1512	gi|489772257|	glutamine amidotransferase [Lactobacillus	100	235	0	0	1	705	1	235	6.12E−174	476
	ref|WP_003676161.1|	reuteri]
TBCP-1560_g_1513	gi|489763609|	hypothetical protein [Lactobacillus reuteri]	99	100	1	0	1	300	1	100	1.60E−49	151
	ref|WP_003667545.1|
TBCP-1560_g_1519	gi|489762592|	hypothetical protein [Lactobacillus reuteri]	87.94	199	24	0	1	597	1	199	7.48E−53	167
	ref|WP_003666530.1|
TBCP-1560_g_1522	gi|970363739|	Protein of unknown function DUF1085	100	198	0	0	1	594	1	198	1.54E−149	412
	emb|CUR37248.1|	[Lactobacillus reuteri]
TBCP-1560_g_1527	gi|489767250|	hypothetical protein [Lactobacillus reuteri]	83.607	61	10	0	1	183	1	61	7.36E−23	81.3
	ref|WP_003671176.1|
TBCP-1560_g_1528	gi|1017201547|	hypothetical protein [Lactobacillus reuteri]	66.816	223	56	1	1	669	1	205	1.54E−99	286
	ref|WP_063164220.1|
TBCP-1560_g_1529	gi|970369384|	hypothetical protein LRLP16767_LR202_02008	97.802	91	2	0	1	273	1	91	7.44E−65	189
	emb|CUR42281.1|	[Lactobacillus reuteri]
TBCP-1560_g_1530	gi|1017201549|	hypothetical protein [Lactobacillus reuteri]	94.382	89	5	0	46	312	16	104	1.26E−59	177
	ref|WP_063164222.1|
TBCP-1560_g_1531	gi|1017201497|	hypothetical protein [Lactobacillus reuteri]	98.551	69	1	0	1	207	1	69	2.53E−45	138
	ref|WP_063164170.1|
TBCP-1560_g_1535	gi|948612947|	hypothetical protein FC53_GL001662 [Lactobacillus	98.496	133	2	0	1	399	14	146	2.76E−97	275
	gb|KRK45104.1|	reuteri DSM 20016]
TBCP-1560_g_1537	gi|1017201557|	hypothetical protein [Lactobacillus reuteri]	84.746	59	9	0	1	177	1	59	6.44E−32	103
	ref|WP_063164230.1|
TBCP-1560_g_1539	gi|512156405|	Na+/H+ antiporter [Lactobacillus reuteri	99.359	156	1	0	1	468	1	156	1.40E−103	292
	gb|AGN99688.1|	I5007]
TBCP-1560_g_1551	gi|970364845|	Xylose-responsive transcription regulator,	100	242	0	0	13	738	67	308	1.50E−179	494
	emb|CUR36302.1|	ROK family [Lactobacillus reuteri]
TBCP-1560_g_1574	gi|489772332|	hypothetical protein [Lactobacillus reuteri]	100	140	0	0	1	420	1	140	8.00E−104	291
	ref|WP_003676235.1|
TBCP-1560_g_1579	gi|489763547|	hypothetical protein [Lactobacillus reuteri]	99.485	194	1	0	1	582	1	194	1.62E−143	396
	ref|WP_003667483.1|
TBCP-1560_g_1589	gi|489762497|	type I glyceraldehyde-3-phosphate dehydrogenase	100	335	0	0	1	1005	1	335	0	688
	ref|WP_003666436.1|	[Lactobacillus reuteri]
TBCP-1560_g_1590	gi|737183747|	hypothetical protein [Lactobacillus reuteri]	100	39	0	0	7	123	24	62	1.06E−22	79.7
	ref|WP_035169635.1|
TBCP-1560_g_1592	gi|512739358|	MFS transporter [Lactobacillus reuteri]	100	451	0	0	1	1353	1	451	0	813
	ref|WP_016497054.1|
TBCP-1560_g_1594	gi|183224377|	hypothetical protein [Lactobacillus reuteri	100	43	0	0	1	129	1	43	2.07E−24	84
	dbj|BAG24894.1|	JCM 1112]
TBCP-1560_g_1595	gi|227071512|	hypothetical protein HMPREF0535_0374	96.429	56	2	0	1	168	1	56	1.21E−33	107
	gb|EEI09811.1|	[Lactobacillus reuteri MM2-3]
TBCP-1560_g_1611	gi|953265697|	Lactate utilization protein A [Lactobacillus	100	298	0	0	1	894	1	298	0	595
	emb|CUU13083.1|	reuteri ATCC 53608]
TBCP-1560_g_1626	gi|512739368|	YigZ family protein [Lactobacillus reuteri]	100	210	0	0	1	630	1	210	5.01E−157	432
	ref|WP_016497064.1|
TBCP-1560_g_1637	gi|489763487|	hypothetical protein [Lactobacillus reuteri]	100	59	0	0	1	177	1	59	1.11E−37	118
	ref|WP_003667423.1|
TBCP-1560_g_1647	gi|512739377|	pyrroline-5-carboxylate reductase [Lactobacillus	100	257	0	0	1	771	1	257	0	513
	ref|WP_016497072.1|	reuteri]
TBCP-1560_g_1666	gi|183224312|	putative glutaredoxin [Lactobacillus reuteri	100	73	0	0	1	219	3	75	1.64E−51	154
	dbj|BAG24829.1|	JCM 1112]
TBCP-1560_g_1667	gi|489772428|	ribonucleoside-diphosphate reductase subunit	99.862	723	1	0	1	2169	1	723	0	1501
	ref|WP_003676331.1|	alpha [Lactobacillus reuteri]
TBCP-1560_g_1669	gi|489772430|	bifunctional acetaldehyde-CoA/alcohol dehydrogenase	100	878	0	0	1	2634	1	878	0	1787
	ref|WP_003676333.1|	[Lactobacillus reuteri]
TBCP-1560_g_1676	gi|489772438|	hypothetical protein [Lactobacillus reuteri]	98.684	76	1	0	1	228	1	76	3.34E−52	156
	ref|WP_003676341.1|
TBCP-1560_g_1688	gi|489762353|	50S ribosomal protein L33 [Lactobacillus	100	49	0	0	1	147	1	49	4.99E−32	103
	ref|WP_003666293.1|	reuteri]
TBCP-1560_g_1695	gi|337729049|	hypothetical protein LRATCC53608_1419	100	98	0	0	1	294	1	98	9.06E−70	202
	emb|CCC04172.1|	[Lactobacillus reuteri ATCC 53608]
TBCP-1560_g_1704	gi|970363458|	FIG00746641: hypothetical protein [Lactobacillus	99.83	589	1	0	1	1767	1	589	0	1107
	emb|CUR37571.1|	reuteri]
TBCP-1560_g_1719	gi|337729072|	hypothetical protein LRATCC53608_1442	100	77	0	0	1	231	1	77	8.45E−52	155
	emb|CCC04195.1|	[Lactobacillus reuteri ATCC 53608]
TBCP-1560_g_1721	gi|512156590|	NADH oxidase [Lactobacillus reuteri I5007]	100	48	0	0	1	144	1	48	6.82E−31	100
	gb|AGN99873.1|
TBCP-1560_g_1725	gi|1017201595|	MarR family transcriptional regulator [Lactobacillus	100	122	0	0	1	366	1	122	3.54E−89	253
	ref|WP_063164268.1|	reuteri]
TBCP-1560_g_1727	gi|489772083|	2-Cys peroxiredoxin [Lactobacillus reuteri]	100	166	0	0	1	498	1	166	7.45E−123	342
	ref|WP_003675988.1|
TBCP-1560_g_1732	gi|489770765|	hypothetical protein [Lactobacillus reuteri]	100	107	0	0	1	321	1	107	2.08E−74	226
	ref|WP_003674676.1|
TBCP-1560_g_1733	gi|489770765|	hypothetical protein [Lactobacillus reuteri]	100	348	0	0	1	1044	123	470	0	674
	ref|WP_003674676.1|
TBCP-1560_g_1736	gi|1017201597|	hypothetical protein [Lactobacillus reuteri]	100	727	0	0	1	2181	22	748	0	1196
	ref|WP_063164270.1|
TBCP-1560_g_1738	gi|737175312|	hypothetical protein [Lactobacillus reuteri]	100	62	0	0	1	186	1	62	7.72E−40	124
	ref|WP_035161508.1|
TBCP-1560_g_1739	gi|512739413|	mucus-binding protein [Lactobacillus reuteri]	88.861	1185	116	9	1	3543	1	1173	0	1920
	ref|WP_016497108.1|
TBCP-1560_g_1739	gi|512739413|	mucus-binding protein [Lactobacillus reuteri]	99.567	693	3	0	10978	13056	570	1262	0	1300
	ref|WP_016497108.1|
TBCP-1560_g_1739	gi|512739413|	mucus-binding protein [Lactobacillus reuteri]	78.734	616	115	9	2326	4161	570	1173	0	917
	ref|WP_016497108.1|
TBCP-1560_g_1739	gi|512739413|	mucus-binding protein [Lactobacillus reuteri]	78.734	616	115	9	2944	4779	570	1173	0	917
	ref|WP_016497108.1|
TBCP-1560_g_1739	gi|512739413|	mucus-binding protein [Lactobacillus reuteri]	78.734	616	115	9	3562	5397	570	1173	0	917
	ref|WP_016497108.1|
TBCP-1560_g_1739	gi|512739413|	mucus-binding protein [Lactobacillus reuteri]	78.734	616	115	9	4180	6015	570	1173	0	917
	ref|WP_016497108.1|
TBCP-1560_g_1739	gi|512739413|	mucus-binding protein [Lactobacillus reuteri]	78.734	616	115	9	4798	6633	570	1173	0	917
	ref|WP_016497108.1|
TBCP-1560_g_1739	gi|512739413|	mucus-binding protein [Lactobacillus reuteri]	78.734	616	115	9	5416	7251	570	1173	0	917
	ref|WP_016497108.1|
TBCP-1560_g_1739	gi|512739413|	mucus-binding protein [Lactobacillus reuteri]	78.734	616	115	9	6034	7869	570	1173	0	917
	ref|WP_016497108.1|
TBCP-1560_g_1739	gi|512739413|	mucus-binding protein [Lactobacillus reuteri]	78.734	616	115	9	6652	8487	570	1173	0	917
	ref|WP_016497108.1|
TBCP-1560_g_1739	gi|512739413|	mucus-binding protein [Lactobacillus reuteri]	78.734	616	115	9	7270	9105	570	1173	0	917
	ref|WP_016497108.1|
TBCP-1560_g_1739	gi|512739413|	mucus-binding protein [Lactobacillus reuteri]	78.734	616	115	9	7888	9723	570	1173	0	917
	ref|WP_016497108.1|
TBCP-1560_g_1739	gi|512739413|	mucus-binding protein [Lactobacillus reuteri]	78.734	616	115	9	8506	10341	570	1173	0	917
	ref|WP_016497108.1|
TBCP-1560_g_1739	gi|512739413|	mucus-binding protein [Lactobacillus reuteri]	78.734	616	115	9	9124	10959	570	1173	0	917
	ref|WP_016497108.1|
TBCP-1560_g_1739	gi|512739413|	mucus-binding protein [Lactobacillus reuteri]	78.734	616	115	9	9742	11577	570	1173	0	917
	ref|WP_016497108.1|
TBCP-1560_g_1739	gi|512739413|	mucus-binding protein [Lactobacillus reuteri]	78.734	616	115	9	10360	12195	570	1173	0	917
	ref|WP_016497108.1|
TBCP-1560_g_1739	gi|512739413|	mucus-binding protein [Lactobacillus reuteri]	38.164	207	112	9	12211	12795	569	771	2.66E−22	105
	ref|WP_016497108.1|
TBCP-1560_g_1754	gi|337728141|	conserved hypothetical protein [Lactobacillus	100	212	0	0	1	636	1	212	1.63E−127	357
	emb|CCC03232.1|	reuteri ATCC 53608]
TBCP-1560_g_1762	gi|489761688|	cytochrome c554 [Lactobacillus reuteri]	100	46	0	0	1	138	1	46	4.72E−12	52.8
	ref|WP_003665629.1|
TBCP-1560_g_1765	gi|489761683|	D-alanine--poly(phosphoribitol) ligase subunit	100	79	0	0	1	237	1	79	5.13E−53	158
	ref|WP_003665624.1|	2 [Lactobacillus reuteri]
TBCP-1560_g_1778	gi|512156647|	phosphoenolpyruvate synthase [Lactobacillus	100	578	0	0	1	1734	1	578	0	1214
	gb|AGN99930.1|	reuteri I5007]
TBCP-1560_g_1787	gi|512739432|	lipoate--protein ligase A [Lactobacillus reuteri]	99.635	274	1	0	1	822	1	274	0	533
	ref|WP_016497126.1|
TBCP-1560_g_1804	gi|337728093|	conserved hypothetical protein [Lactobacillus	100	41	0	0	1	123	1	41	1.57E−24	84
	emb|CCC03183.1|	reuteri ATCC 53608]
TBCP-1560_g_1810	gi|512739440|	DNA (cytosine-5-)-methyltransferase [Lactobacillus	100	319	0	0	1	957	1	319	0	658
	ref|WP_016497134.1|	reuteri]
TBCP-1560_g_1816	gi|512739446|	endonuclease III [Lactobacillus reuteri]	100	213	0	0	1	639	1	213	6.68E−162	444
	ref|WP_016497140.1|
TBCP-1560_g_1817	gi|512739447|	FMN-binding domain protein [Lactobacillus	100	92	0	0	1	276	5	96	1.59E−64	188
	ref|WP_016497141.1|	reuteri]
TBCP-1560_g_1822	gi|512156691|	transposase [Lactobacillus reuteri I5007]	100	34	0	0	1	102	14	47	1.32E−19	71.2
	gb|AGN99974.1|
TBCP-1560_g_1824	gi|489767413|	ABC transporter substrate-binding protein	100	283	0	0	1	849	1	283	0	572
	ref|WP_003671339.1|	[Lactobacillus reuteri]
TBCP-1560_g_1826	gi|112944237|	ABC-type metal ion transport system, permease	100	229	0	0	1	687	1	229	7.61E−106	304
	gb|ABI26339.1|	component [Lactobacillus reuteri]
TBCP-1560_g_1827	gi|489761558|	succinyl-diaminopimelate desuccinylase	99.738	381	1	0	1	1143	1	381	0	784
	ref|WP_003665499.1|	[Lactobacillus reuteri]
TBCP-1560_g_1828	gi|970364154|	hypothetical protein LRLP16767_LRPG3B_00672	100	36	0	0	1	108	4	39	1.71E−08	43.1
	emb|CUR36880.1|	[Lactobacillus reuteri]
TBCP-1560_g_1852	gi|512739465|	esterase [Lactobacillus reuteri]	100	180	0	0	1	540	1	180	2.49E−134	372
	ref|WP_016497159.1|
TBCP-1560_g_1854	gi|970363126|	Cobalt-zinc-cadmium resistance protein	97.761	134	3	0	1	402	1	134	1.46E−96	273
	emb|CUR37858.1|	[Lactobacillus reuteri]
TBCP-1560_g_1855	gi|512156721|	cobalt-zinc-cadmium resistance protein [Lactobacillus	99.057	212	2	0	1	636	1	212	2.81E−137	382
	gb|AGO00005.1|	reuteri I5007]
TBCP-1560_g_1860	gi|489770581|	short-chain dehydrogenase [Lactobacillus	98.707	232	3	0	43	738	15	246	8.47E−171	469
	ref|WP_003674492.1|	reuteri]
TBCP-1560_g_1864	gi|489770573|	cyclic pyranopterin monophosphate synthase	100	162	0	0	1	486	1	162	4.27E−118	329
	ref|WP_003674484.1|	accessory protein [Lactobacillus reuteri]
TBCP-1560_g_1868	gi|970370004|	integrase [Lactobacillus reuteri]	100	411	0	0	1	1233	1	411	0	865
	emb|CUR41367.1|
TBCP-1560_g_1869	gi|489761612|	hypothetical protein [Lactobacillus reuteri]	66.667	60	20	0	1	180	1	60	4.55E−26	89.4
	ref|WP_003665553.1|
TBCP-1560_g_1870	gi|970370006|	hypothetical protein LRLP16767_LR202_01424	98.857	175	2	0	1	525	1	175	3.54E−130	361
	emb|CUR41369.1|	[Lactobacillus reuteri]
TBCP-1560_g_1872	gi|970370008|	hypothetical protein LRLP16767_LR202_01426	99.582	958	4	0	1	2874	1	958	0	1981
	emb|CUR41371.1|	[Lactobacillus reuteri]
TBCP-1560_g_1873	gi|970370009|	hypothetical protein LRLP16767_LR202_01427	100	122	0	0	1	366	1	122	1.50E−87	249
	emb|CUR41372.1|	[Lactobacillus reuteri]
TBCP-1560_g_1876	gi|503689749|	lysozyme M1 (1,4-beta-N-acetylmuramidase)	86.047	129	18	0	187	573	1	129	6.65E−81	237
	ref|WP_013923825.1|	[Lactobacillus reuteri]
TBCP-1560_g_1877	gi|489759748|	spore coat protein CotH [Lactobacillus reuteri]	26.557	305	188	9	10	888	1058	1338	1.70E−19	88.2
	ref|WP_003663696.1|
TBCP-1560_g_1882	gi|970369249|	hypothetical protein LRLP16767_LR202_02167	94.258	209	12	0	1	627	81	289	8.41E−120	340
	emb|CUR42509.1|	[Lactobacillus reuteri]
TBCP-1560_g_1883	gi|915462620|	mobilization protein [Lactobacillus reuteri]	97.512	402	10	0	1	1206	11	412	0	738
	ref|WP_050802153.1|
TBCP-1560_g_1884	gi|489770545|	hypothetical protein [Lactobacillus reuteri]	95.408	196	9	0	1	588	1	196	4.22E−127	355
	ref|WP_003674456.1|
TBCP-1560_g_1908	gi|489772006|	3,4-dihydroxy-2-butanone-4-phosphate synthase	100	220	0	0	1	660	1	220	6.96E−165	452
	ref|WP_003675911.1|	[Lactobacillus reuteri]
TBCP-1560_g_1909	gi|970364398|	Beta-phosphoglucomutase [Lactobacillus reuteri]	99.531	213	1	0	1	639	1	213	2.61E−144	400
	emb|CUR36668.1|
TBCP-1560_g_1944	gi|970364175|	hypothetical protein LRLP16767_LRPG3B_00662	100	37	0	0	1	111	1	37	7.76E−23	79.3
	emb|CUR36870.1|	[Lactobacillus reuteri]
TBCP-1560_g_1953	gi|512739498|	branched-chain amino acid transporter [Lactobacillus	100	107	0	0	1	321	1	107	2.65E−74	214
	ref|WP_016497191.1|	reuteri]
TBCP-1560_g_1954	gi|490698967|	branched-chain amino acid transporter AzlC	100	230	0	0	1	690	1	230	5.57E−139	388
	ref|WP_004562469.1|	[Lactobacillus reuteri]
TBCP-1560_g_1958	gi|337729002|	hypothetical protein LRATCC53608_1370	97.826	46	1	0	1	138	1	46	1.12E−26	89.7
	emb|CCC04122.1|	[Lactobacillus reuteri ATCC 53608]
TBCP-1560_g_1968	gi|512739505|	RNA-binding protein [Lactobacillus reuteri]	100	255	0	0	1	765	1	255	0	521
	ref|WP_016497198.1|
TBCP-1560_g_1969	gi|970370437|	Inner membrane protein translocase component	100	263	0	0	1	789	15	277	2.91E−158	440
	emb|CUR40976.1|	YidC, short form OxaI-like [Lactobacillus
		reuteri]
TBCP-1560_g_1970	gi|489761281|	ribonuclease P protein component [Lactobacillus	100	117	0	0	1	351	1	117	6.87E−84	239
	ref|WP_003665224.1|	reuteri]

The beneficial technical effects of the present disclosure are as follows.

The strain CCFM8631 of Lactobacillus reuteri of the present disclosure significantly increases neurotransmitter 5-hydroxytryptamine (5-HT) level in peripheral blood of rat, regulates brain-gut axis, relieves mental illnesses related to metabolic syndrome, for example anxiety, depression and so on, recovers the hormone level, for example testosterone and so on in peripheral blood of rat caused by high-fat high-starch diet, recovers abundances of Bifidobacterium genus, Turicibacter genus, Oscillospira genus and Blautia genus in abnormal intestinal flora of rat affected by high-fat high-starch diet. In addition, strain CCFM8631 of Lactobacillus reuteri has pretty good tolerance to simulated gastrointestinal fluid, and quickly colonizes in intestinal, significantly alleviates pathology damages of tissues, such as liver, duodenum and so on of rat with metabolic syndrome caused by high-fat high-starch diet; significantly improves oral glucose tolerance of rat with metabolic syndrome and decreases the under curve area of glucose tolerance test; significantly increases triglyceride and total cholesterol levels in serum of rat with metabolic syndrome caused by high-fat high-starch diet. The strain CCFM8631 of Lactobacillus reuteri of the present disclosure can be used to prepare health foods or medicines that improve metabolic syndrome, regulates intestinal flora, relieves irritable bowel syndrome, regulates brain-gut axis and alleviates mental illness such as anxiety, depression and so on, which has a pretty wide application prospect.

In order to understand the present disclosure further, the technical solutions in the examples of the present disclosure will be described clearly and completely herein in conjunction with the examples of the present disclosure. Apparently, the described examples are only a part of the examples of the present disclosure, rather than all examples. Based on the examples in the present disclosure, all of other examples, made by one of ordinary skill in the art without any creative efforts, fall into the protection scope of the present disclosure.

All of the reagents related to examples of the present disclosure are commercial products without special description, which can be purchased on market. All of the following examples are completed by theory and technology research group of probiotics of Research Center of Food Biotechnology in School of Food Science and Technology, Jiangnan University.

Example 1: Strain CCFM8631 of Lactobacillus reuteri has Good Tolerance to Simulated Gastrointestinal Fluid

The cryopreserved strain CCFM8631 of Lactobacillus reuteri were inoculated in the MRS medium and cultured at 37° C. for 48 hours under anaerobic cultivation, followed by 2 to 3 times subculture in MRS liquid medium. The medium with strain CCFM8631 of Lactobacillus reuteri was taken and centrifuged for 5 minutes at a speed of 8000 g, and then resuspended (1:1) in an artificial simulated gastric juice (MRS medium containing 1% pepsin, pH 2.5), followed by anaerobic cultivation at 37° C. Sampling was carried out at 0 hour and 3 hours, and the samples were cultured on MRS medium agar plate for colony counting. The viability numbers were counted and the survival rates were calculated. The survival rate is the rate of the viable count at the desired time point to the viable count at the 0 hour, which was expressed in %.

The medium with cultured strain CCFM8631 of Lactobacillus reuteri was taken and centrifuged at a speed of 8000×g for 5 minutes. The bacteria were collected and resuspened (1:1) in artificial simulated intestinal fluid (MRS medium containing 0.3% bile salt from ox, 1% trypsin, pH 8.0), followed by anaerobic cultivation at 37° C. Sampling was carried out at 0 hour and 4 hours, and the samples were cultured on MRS medium agar plate for colony counting. The viability numbers were counted and the survival rates were calculated. The survival rate is the rate of the viable count at the desired time point to the viable count at the 0 hour, which was expressed in %.

The experiment results were shown in Table 2. The results showed that strain CCFM8631 of Lactobacillus reuteri has a relative good tolerance to simulated gastrointestinal fluid.

TABLE 2
Tolerance of strain CCFM8631 of Lactobacillus reuteri
to simulated gastrointestinal fluid
	Simulated	Simulated
	Gastric Fluid	Gastric Fluid
	Treatment Time (h)	3	4
	Survival Rate (%)	88.1 ± 5.3	67.5 ± 7.2

Example 2: Strain CCFM8631 of Lactobacillus reuteri has No Toxic and Side Effects on SD Rat

The strain CCFM8631 of Lactobacillus reuteri bacteria were resuspended in 2% sucrose solution to give a bacterial suspension with a concentration of 3.0×10⁹ CFU/mL. 8 healthy male SD rats with a weight between 180 and 200 g were chosen and acclimated for 1 week before experiments. The rats were administered with the above bacteria suspension by intragastric gavage once daily at a dose of 2 mL/day/rat. The death and weight of the rats were observed and recorded for one week. The results were shown in Table 3.

TABLE 3
Death and changes of body weight in rats
	Time (day)
	1	2	3	4	5	6	7
Weight (g)	228.2 ± 0.9	235.3 ± 1.1	241.1 ± 0.5	246.7 ± 0.7	252.0 ± 0.2	258.2 ± 1.3	264.5 ± 0.4
Death	—	—	—	—	—	—	—
Comment:
“—”, no death.

The results showed that administration of strain CCFM8631 of Lactobacillus reuteri with a concentration of 3.0×10⁹ CFU/mL did not have significant influences on rats, there was no significant change on the body weight and no death. There were no obvious pathological symptoms in the appearance of the rats.

Example 3: Strain CCFM8631 of Lactobacillus reuteri has Good Recovery Effect on Tissue Damages of Liver, Duodenum and so on in Rats with Metabolic Syndrome

48 healthy male SD rats with weight from 180 to 200 g were chosen and acclimated for 1 week. The rats were divided into 6 groups randomly: non-specific control group (NC), high-fat high-starch (HFHS) diet model control group, simvastatin control group (SC), rosiglitazone hydrochloride control group (RH), strain CCFM8631 of Lactobacillus reuteri intervention group (CCFM8631), Lactobacillus rhamnosus LGG control group (LGG), 8 rats per group. The rats were administered with the bacteria suspension (3.0×10⁹ CFU/mL, in 2% sucrose solution) by intragastric gavage. Grouping and treatment method were shown in Table 4.

TABLE 4
Grouping and treatment method of the experiment
	Number			Treatment Method:
	of rats/	Treatment		administrated by intra-
Group	Group	Duration	Feed	gastric gavage daily
NC	8	12 Weeks	Normal feed	2 ml of 2% sucrose
				solution
HFHS	8	12 Weeks	High-fat	2 ml of 2% sucrose
			high-starch	solution
			feed
SC	8	12 Weeks	High-fat	2 ml of 2% sucrose
			high-starch	solution containing 3
			feed	mg/kg/BW/d of
				simvastatin
RH	8	12 Weeks	High-fat	2 ml of 2% sucrose
			high-starch	solution containing 10
			feed	mg/kg/BW/d of
				rosiglitazone
				hydrochloride
CCFM8631	8	12 Weeks	High-fat	2 ml of 2% sucrose
			high-starch	solution containing
			feed	3.0 × 109 CFU/mL
				of CCFM8631
LGG	8	12 Weeks	High-fat	2 ml of 2% sucrose
			high-starch	solution containing
			feed	3.0 × 109 CFU/mL
				of LGG of

At the end of the experiment, the rats were fasted (with access to water) for 12 hours. After administering 10% chloral hydrate by peritoneal injection, the rats were anesthetized, the blood samples were collected from the hearts, and the rats were sacrificed by cervical dislocation. The blood samples were centrifuged at a speed of 3000×g at 4° C. for 10 minutes. The supernatant was collected and frozen at −80° C. for later use. Liver, duodenum and so on were collected and quickly put into ice-cold physiological saline to wash away the blood, followed by fixation in paraformaldehyde. In addition, small intestine was collected and immediately frozen in liquid nitrogen.

Intestine, duodenum and so on were taken and prepared as paraffin sections, followed by HE straining. Morphology of the tissues were observed and imaged under optical microscope for pathological evaluation. The results were shown in FIGS. 2 and 3. The HE straining was performed by the following steps.

(1) Fixation: the tissue samples were washed with physiological saline and immediately put into neutral paraformaldehyde solution (4%) for fixation, and the duration of fixation was generally within 72 hours.

(2) Washing: the tissue samples were washed with running water or immersed in water for a few hours or overnight.

(3) Dehydration: the tissue samples were dehydrated by successively immersing in ethanol solutions of 70%, 80% and 90%, each for 30 minutes, and then immersing in 95% ethanol solution once for 20 minutes, immersing in 100% ethanol solution twice, each time for 10 minutes.

(4) Transparency: the tissue samples were immersed in a mixture of ½ absolute ethanol and ½ xylene for 10 minutes, xylene I for 10 minutes, and xylene II for 10 minutes (until the samples became transparent).

(5) Waxing: the tissue samples were placed in paraffin (at 62° C.) for 2 hours.

(6) Embedding: the largest side of the sample was placed in the bottom so that the sections have the largest tissue surface.

(7) Cutting: the wax blocks were cut by a manually operating microtome into slices with a thickness of 5 μm.

(8) Floatation and adhesion of sections (slice-salvaging): a water bath was used and the water was maintained at 42° C.; sections were placed onto the water surface smoothly.

(9) Drying: slides and slide rack were put into a 55° C. drying oven for about 2 hours until the wax melted.

(10) Hydration: slides were immersed in xylene I and II for 10 minutes respectively for dewaxing, and then immersed in ethanol solutions of 100%, 95%, 90%, 80% and 70% for 5 minutes respectively, and then immersed in distilled water for 3 minutes.

(11) Primary strain: the slides were put into hematoxylin solution and strained for about 20 seconds.

(12) Washing: the slides were washed with tap water for about 15 minutes until the slices became blue. Pay attention to the water flow to avoid the sections detaching from the slide.

(13) Differentiation: the slides were put into ethanol solution with 1% hydrochloric acid for 7 seconds until the slices turned red (the color became light).

(14) Rinsing: the slides were washed with tap water for 15 to 20 minutes until the color recovered blue.

(15) Re-stain: the slides were immersed in eosin solution and immediately taken out for dehydration.

(16) Dehydration: the slides were immersed in 95% ethanol solution I, 95% ethanol solution II and 70% ethanol solution successively, followed by immersing in 80% ethanol solution for 50 seconds and absolute ethanol for 2 minutes.

(17) Transparency: the slides were immersed in ½ of absolute ethanol and ½ of xylene for 1 minute, xylene I for 2 minutes and xylene II for 2 minutes, respectively.

(18) Sealing: after the treatment of xylene, the neutral balsam was used as mounting medium, which could be diluted to appropriate consistency with xylene.

FIG. 2 showed that high-fat high-starch diet caused hepatocyte microvesicular steatosis, and a number of rats have infiltration of inflammatory cell and hyperplasia of fibrous tissue. In high-fat high-starch diet model control group (HFHS), there was significant hyperplasia of fibrous tissue in liver tissue and morphologic features of early fibrosis. In strain CCFM8631 of Lactobacillus reuteri intervention group (CCFM8631), administration of strain CCFM8631 of Lactobacillus reuteri by intragastric gavage significantly improved the lesions above, and the effects were significantly better than that of group LGG FIG. 3 showed that under optical microscope, lesions of duodenum were villi broadening, interstitial edema, increase of inflammatory cells and increase of interstitial macrophages in a few cases. In strain CCFM8631 of Lactobacillus reuteri intervention group (CCFM8631), administration of strain CCFM8631 of Lactobacillus reuteri by intragastric gavage improved the lesions above, and the effects were significantly better than that of group LGG.

Example 4: Strain CCFM8631 of Lactobacillus reuteri has Recovery Effect on Intestinal Flora Imbalance Caused by High-Fat High-Starch Diet

Grouping, molding and treatment processes using SD rats were the same as described in Example 3. Before the end of the experiment, fresh feces of the rats were taken and metagenome samples were extracted. A second-generation sequencer was used for sequencing and the microbial community structure was analyzed.

The experiment results were shown in FIG. 4. In feces of high-fat high-starch diet model control group (HFHS), relative abundances of intestinal microbes Bifidobacterium genus and Turicibacter genus significantly decreased. In strain CCFM8631 of Lactobacillus reuteri intervention group (CCFM8631), intake of strain CCFM8631 of Lactobacillus reuteri leaded to a significant recovery of the relative abundances of these two genera, while drugs and LGG did not show recovery effect on the abundances of these two genera intestinal microbes. In addition, in rat feces of the high-fat high-starch diet model control group (HFHS), the relative abundance of intestinal microbes of Oscillospira genes and Blautia genus significantly increased. While in strain CCFM8631 of Lactobacillus reuteri intervention group (CCFM8631), intake of strain CCFM8631 of Lactobacillus reuteri regulated the abundance of Blautia genus back to normal level, and the effects were better than that of the drugs and LGG.

Example 5: Strain CCFM8631 of Lactobacillus reuteri Reduced (Fasting) Blood Glucose Level of Rats with Metabolic Syndrome

Grouping, molding and treatment processes using SD rats were the same as described in Example 3.

At the end of the experiment the rats were fasted (with access to water) for 12 hours and fasting blood glucose level of the rats was tested. The results were shown in FIG. 5.

In high-fat high-starch diet model control group (HFHS), fasting blood glucose level of rats significantly increased. In strain CCFM8631 of Lactobacillus reuteri intervention group (CCFM8631), administration of strain CCFM8631 of Lactobacillus reuteri by intragastric gavage significantly decreased fasting blood glucose level of model rats, approximately to non-specific control group, and its ability to decrease fasting blood glucose level of rat is better than that of rosiglitazone hydrochloride control group (RH) and Lactobacillus rhamnosus LGG control group (LGG).

Example 6: Strain CCFM8631 of Lactobacillus reuteri Increased Glucose Tolerance of Rat with Metabolic Syndrome

Grouping, molding and treatment processes using SD rats were the same as described in Example 3. At the end of the experiment, the rats were fasted (with access to water) for 12 hours. Glucose solution (2 g/kg) was injected by intraperitoneal injection and the blood glucose level was measured at 0, 30, 60 and 120 minutes. The experiment results were shown in FIGS. 6 and 7.

As shown in FIG. 6, glucose tolerance of rats in high-fat high-starch diet model control group (HFHS) was poor. After administration of glucose by intragastric gavage, blood glucose level rose significantly and decreased slowly. As shown in FIG. 7, in strain CCFM8631 of Lactobacillus reuteri intervention group (CCFM8631), administration of strain CCFM8631 of Lactobacillus reuteri by intragastric gavage significantly decreased AUC_glucose area, and there was no significant difference comparing with that of rosiglitazone hydrochloride control group (RH) and non-specific control group (NC). This indicated that strain CCFM8631 of Lactobacillus reuteri significantly improves oral glucose tolerance, and the effect was better than that of Lactobacillus rhamnosus LGG These results were consistent with that of the blood glucose indexes, indicating that strain CCFM8631 of Lactobacillus reuteri further decreased blood glucose level by increasing glucose tolerance.

Example 7: Strain CCFM8631 of Lactobacillus reuteri Decreased Total Cholesterol (TC) Level in Serum of Rat with Metabolic Syndrome

Grouping, molding and treatment processes using SD rats were the same as described in Example 3. At the end of the experiment, the rats were fasted (with access to water) for 12 hours. After administering 10% chloral hydrate by peritoneal injection for anesthetizing, blood sample was collected from the heart, and the rats were sacrificed by cervical dislocation. The blood samples were centrifuged at a speed of 3000×g at 4° C. for 10 minutes, and the supernatant was collected. The total cholesterol (TC) in the blood was measured according to the protocol of the detection kit. The experiment results were shown in FIG. 8.

As shown in FIG. 8, total cholesterol in serum of rats in high-fat high-starch diet model control group (HFHS) significantly increased. In strain CCFM8631 of Lactobacillus reuteri intervention group (CCFM8631), administration of strain CCFM8631 of Lactobacillus reuteri by intragastric gavage decreased level of total cholesterol in serum.

Example 8: Strain CCFM8631 of Lactobacillus reuteri Decreased Triglyceride (TG) Level in Serum of Metabolic Syndrome Rat

Grouping, molding and treatment processes using SD rats were the same as described in Example 3. At the end of the experiment, the rats were fasted (with access to water) for 12 hours. After administering 10% chloral hydrate by peritoneal injection for anesthetizing, blood sample was collected from the heart, and the rats were sacrificed by cervical dislocation. The blood samples were centrifuged at a speed of 3000×g at 4° C. for 10 minutes, and the supernatant was collected. The triglyceride (TG) level in the blood was measured according to the protocol of the detection kit. The experiment results were shown in FIG. 9.

As shown in the experiment results, comparing with non-specific control group (NC), triglyceride level in serum of rats in high-fat high-starch diet model control group significantly increased. In strain CCFM8631 of Lactobacillus reuteri intervention group, administration of strain CCFM8631 of Lactobacillus reuteri by intragastric gavage decreased triglyceride level in serum, and the effect was better than that of LGG

Example 9: Strain CCFM8631 of Lactobacillus reuteri Affected 5-HT and Testosterone Levels in Serum of Rat with Metabolic Syndrome

Grouping, molding and treatment processes using SD rats were the same as described in Example 3. At the end of the experiment, the rats were fasted (with access to water) for 12 hours. After administering 10% chloral hydrate by peritoneal injection for anesthetizing, blood sample was collected from the heart, and the rats were sacrificed by cervical dislocation. The blood samples were centrifuged at a speed of 3000×g at 4° C. for 10 minutes, and the supernatant was collected. The 5-HT and testosterone levels in the blood were measured according to the protocol of the detection kit.

The experiment results were shown in FIG. 10. As shown in the experiment results, strain CCFM8631 of Lactobacillus reuteri significantly increased 5-HT level in serum of rats, while LGG has no significant improvement on 5-HT level. Comparing with non-specific control group (NC), testosterone level in serum of rats in high-fat high-starch diet model control group (HFHS) significantly increased. In strain CCFM8631 of Lactobacillus reuteri intervention group (CCFM8631), administration of strain CCFM8631 of Lactobacillus reuteri by intragastric gavage reduced the testosterone level in serum back to normal, and LGG can decrease the testosterone level greatly lower than normal level in model rats.

Claims

1. A method of improving metabolic syndrome, modulating intestinal flora, improving irritable bowel syndrome, modulating brain-gut axis, relieving anxiety and/or depression, comprising administering a strain CCFM8631 of Lactobacillus reuteri which is deposited at China General Microbiological Culture Collection Center (CGMCC) with an accession number CGMCC 14394, to a subject in need thereof.

2. The method according to claim 1, wherein the improving of metabolic syndrome is to relieve the symptoms of hyperglycemia and hyperlipidemia, inflammation of liver and duodenum, and liver fibrosis; the modulating of intestinal flora is to normalize abnormal abundances of Blautia genus, Turicibacter genus, Oscillospira genus and Bifidobacterium genus in the intestinal flora; and the modulating of brain-gut axis and the relieving of anxiety and/or depression is to increase 5-hydroxytryptamine level in peripheral blood.

3. A method for preparing a composition, comprising: inoculating the strain CCFM8631 of Lactobacillus reuteri to MRS medium at an inoculum size of 2 to 4 wt %, culturing for 18 to 20 h at 37° C. under anaerobic condition, collecting bacteria, resuspending the bacteria with a protectant to a bacterial density of 1010 CFU/mL, culturing the suspension at 37° C. for 50 to 70 minutes under anaerobic condition, and drying the resulting culture, wherein the strain CCFM8631 is deposited at China General Microbiological Culture Collection Center (CGMCC) with an accession number CGMCC 14394.

4. The method according to claim 3, wherein the protectant is a aqueous solution containing 100 g/L to 150 g/L of nonfat milk powder, 100 g/L to 150 g/L of maltodextrin, and 140 g/L to 160 g/L of trehalose; the drying is vacuum freeze-drying after pre-freezing at −15 to −20° C. for 8 to 14 h.