LARGE-SCALE PRODUCTION OF AVIAN MYELOBLASTOSIS VIRUS

Information

• Research Project
• 3491820

• ApplicationId

  3491820

• Core Project Number

  R43CA045876

• Full Project Number

  1R43CA045876-01

• Serial Number

  45876

• FOA Number

• Sub Project Id

• Project Start Date

  9/30/1987 - 37 years ago

• Project End Date

  3/31/1988 - 36 years ago

• Program Officer Name

• Budget Start Date

  9/30/1987 - 37 years ago

• Budget End Date

  3/31/1988 - 36 years ago

• Fiscal Year

  1987

• Support Year

  1

• Suffix

• Award Notice Date

  9/29/1987 - 37 years ago

Organizations

• MOLECULAR BIOLOGY RESOURCES, INC.

Avian myeloblastosis virus (AMV), a source of reverse transcriptase for use in recombinant DNA work, is currently produced commercially from the plasma of leukemic chicks. Production involves inoculation of several hundred day-old chicks with AMV and harvest of virus-containing blood by heart puncture 7 to 10 days later. The research proposed will investigate the feasibility of commercial-scale production of AMV from cell cultures of chicken myeloblasts. Procedures for in vitro transformation of chicken macrophage lineage cells with AMV and the in vitro growth of the transformed cells on a small scale are well known. Large-scale culture of AMV-transformed myeloblasts has also been achieved, but with specially constructed apparatus. The present proposal discusses adaptation of recent advances in cell culture conditions and apparatus for large-scale cell culture to production of AMV. Successful development of the cell culture method will give a procedure that is both more economical and more humane than the present in vivo method.

IC Name

• Activity

  R43

• Administering IC

  CA

• Application Type

  1

• Direct Cost Amount

• Indirect Cost Amount

• Total Cost

• Sub Project Total Cost

• ARRA Funded

• CFDA Code

  393

• Ed Inst. Type

• Funding ICs

• Funding Mechanism

• Study Section

  SSS

• Study Section Name

• Organization Name

  MOLECULAR BIOLOGY RESOURCES, INC.

• Organization Department

• Organization DUNS

• Organization City

  MILWAUKEE

• Organization State

  WI

• Organization Country

  UNITED STATES

• Organization Zip Code

  53210

• Organization District

  UNITED STATES