LARGE SEQUENCE PAN-CORONAVIRUS VACCINE COMPOSITIONS

FIELD OF THE INVENTION

The present invention relates to vaccines, for example viral vaccines, such as those directed to coronaviruses, e.g., pan-coronavirus vaccines.

BACKGROUND OF THE INVENTION

Over the last two decades, there have been three deadly human outbreaks of Coronaviruses (CoVs) caused by emerging zoonotic CoVs: SARS-CoV, MERS-CoV, and the latest highly transmissible and deadly SARS-CoV-2, which has caused the current COVID-19 global pandemic. All three deadly CoVs originated from bats, the natural hosts, and transmitted to humans via various intermediate animal reservoirs (e.g., pangolins, civet cats and camels). Because there is currently no universal pan-Coronavirus vaccine available, it remains highly possible that other global COVID-like pandemics will emerge in the coming years, caused by yet another spillover of an unknown zoonotic bat-derived SARS-like Coronavirus (SL-CoV) into an unvaccinated human population.

Neutralizing antibodies and antiviral effector CD4⁺ and CD8⁺ T cells appear to be crucial in reducing viral load in the majority of infected asymptomatic and convalescent patients. However, very little information exists on the antigenic landscape and the repertoire of B-cell and CD4⁺ and CD8⁺ T cell epitopes that are conserved among human and bat Coronavirus strains.

SUMMARY OF THE INVENTION

Current vaccines on the market primarily are directed to the spike antigen only and focus on induction of antibody responses. However, this strategy excludes other more conserved antigens of the virus and misses the opportunity to target long-term cross-reactive memory T cell responses. The present invention aims to create a vaccine that not only includes the spike antigen but also includes other conserved antigens of the virus to induce an immune response with both antibodies and T cells.

Determining the antigen and epitope landscapes that are antigenic, immunogenic, protective and conserved among human and animal Coronaviruses as well as the repertoire, phenotype and function of B cells and CD4+ and CD8+ T cells that correlate with resistance seen in asymptomatic COVID-19 patients may inform in the development of future pan-Coronavirus vaccines. The present invention describes using several immuno-informatics and sequence alignment approaches and several immunological assays both in vitro in humans and in vivo in animal models (e.g. mice, hamster and monkeys) to identify several antigenic, immunogenic, protective highly conserved large sequences that include human B cell, CD4+ and CD8+ T cell epitopes that are highly conserved, e.g., highly conserved in: (i) greater than 81,000 SARS-CoV-2 human strains identified in 190 countries on six continents: (ii) six circulating CoVs that caused previous human outbreaks of the “Common Cold”; (iii) nine SL-CoVs isolated from bats; (iv) nine SL-CoV isolated from pangolins; (v) three SL-CoVs isolated from civet cats; and (vi) four MERS strains isolated from camels. Furthermore, the present invention describes the identification of cross-reactive epitopes that: recalled B cell, CD4+ and CD8+ T cells from both COVID-19 patients and healthy individuals who were never exposed to SARS-CoV-2; and induced strong B cell and T cell responses in “humanized” Human Leukocyte Antigen (HLA)-DR1/HLA-A*02:01 double transgenic mice as well as in humans that do not express HLA-DR-1 or HLA-A*02:01 haplotypes. Unlike small epitopes that are restricted to certain HLA haplotype, the large sequences encompass several epitopes restricted to large numbers of HLA haplotypes, thus ascertaining large vaccine coverage of human population regardless of HLA haplotypes and regardless of race and ethnicity.

The present invention is not limited to vaccine compositions for use in humans. The present invention includes vaccine compositions for use in other pet animals such as dogs, cats, etc.

The vaccine compositions herein have the potential to provide lasting B and T cell immunity regardless of Coronaviruses mutations. This may be due at least partly because the vaccine compositions target highly conserved structural and non-structural Coronavirus antigens, such as Coronavirus nucleoprotein (also known as nucleocapsid), in combination with other Coronavirus structural and non-structural antigens with a low mutation rate found in perhaps every human and animal Coronaviruses variants and strains.

The present invention is also related to selecting highly conserved structural (e.g., spike protein) and non-structural Coronavirus antigens inside the virus (e.g., non-spike protein such as nucleocapsid), which may be viral proteins that are normally not necessarily under mutation pressure by the immune system.

The present invention provides pan-Coronavirus recombinant vaccine compositions that induces board, strong and long-lasting B and T cell protective immune responses in humans and pets and animals.

In certain embodiments, the vaccine compositions are for use in humans. In certain embodiments, the vaccine compositions are for use in animals, such as but not limited to mice, cats, dogs, non-human primates, other animals susceptible to coronavirus infection, other animals that may function as preclinical animal models for coronavirus infections, etc.

As used herein, the term “multi-epitope” refers to a composition comprising more than one B and T cell epitope wherein at least: one CD4 and/or CD8 T cell epitope is MHC-restricted and recognized by a TCR, and at least one epitope is a B cell epitope. For example, the vaccine compositions herein may be multi-epitope pan-coronavirus vaccine compositions.

As used herein, the term “recombinant vaccine composition” may refer to one or more proteins or peptides encoded by one or more recombinant genes, e.g. genes that have been cloned into one or more systems that support the expression of said gene(s). The term “recombinant vaccine composition” may refer to the recombinant genes or the system that supports the expression of said recombinant genes.

For example, the present invention provides a pan-coronavirus recombinant vaccine composition comprising one or more large sequences, wherein each of the one or more large sequences comprise at least one of: one or more conserved coronavirus B-cell target epitopes; one or more conserved coronavirus CD4+ T cell target epitopes; and/or one or more conserved coronavirus CD8+ T cell target epitopes; wherein at least one epitope is derived from a non-spike protein.

The present invention also features a pan-coronavirus recombinant vaccine composition, the composition comprising two or more large sequences, wherein each of the two or more large sequences comprise at least one of: one or more conserved coronavirus B-cell target epitopes; one or more conserved coronavirus CD4+ T cell target epitopes; and/or one or more conserved coronavirus CD8+ T cell target epitopes; wherein at least one epitope is derived from a non-spike protein.

The present invention also features a pan-coronavirus recombinant vaccine composition, the composition comprising whole spike protein; and one or both of: one or more conserved coronavirus CD4+ T cell target epitopes; and/or one or more conserved coronavirus CD8+ T cell target epitopes; wherein at least one epitope is derived from a non-spike protein.

The present invention also features a pan-coronavirus recombinant vaccine composition, the composition comprising at least a portion of spike protein, the portion of spike protein comprising a trimerized SARS-CoV-2 receptor-binding domain (RBD); and one or both of: one or more conserved coronavirus CD4+ T cell target epitopes; one or more conserved coronavirus CD8+ T cell target epitopes; wherein at least one epitope is derived from a non-spike protein.

The present invention also features a pan-coronavirus recombinant vaccine composition, the composition comprising whole spike protein; and one or more conserved coronavirus CD4+ T cell target epitopes; and one or more conserved coronavirus CD8+ T cell target epitopes; wherein at least one epitope is derived from a non-spike protein.

The present invention also features a pan-coronavirus recombinant vaccine composition, the composition comprising at least a portion of spike protein, the portion of spike protein comprising a trimerized SARS-CoV-2 receptor-binding domain (RBD); and one or more conserved coronavirus CD4+ T cell target epitopes; and one or more conserved coronavirus CD8+ T cell target epitopes; wherein at least one epitope is derived from a non-spike protein.

The present invention also features a pan-coronavirus recombinant vaccine composition, the composition comprising an antigen delivery system encoding one or more large sequences, wherein each of the one or more large sequences comprise at least one of: one or more conserved coronavirus B-cell target epitopes; one or more conserved coronavirus CD4+ T cell target epitopes: and/or one or more conserved coronavirus CD8+ T cell target epitopes; wherein at least one epitope is from a non-spike protein.

The present invention also features a pan-coronavirus recombinant vaccine composition, the composition comprising an antigen delivery system encoding two or more large sequences, wherein each of the two or more large sequences comprise at least one of: one or more conserved coronavirus B-cell target epitopes; one or more conserved coronavirus CD4+ T cell target epitopes; and/or one or more conserved coronavirus CD8+ T cell target epitopes; wherein at least one epitope is derived from a non-spike protein.

The present invention also features a pan-coronavirus recombinant vaccine composition, the composition comprising an antigen delivery system encoding whole spike protein, and one or both of: one or more conserved coronavirus CD4+ T cell target epitopes; and/or one or more conserved coronavirus CD8+ T cell target epitopes; wherein at least one epitope is derived from a non-spike protein.

The present invention also features a pan-coronavirus recombinant vaccine composition, the composition comprising an antigen delivery system encoding at least a portion of spike protein, the portion of spike protein comprising a trimerized SARS-CoV-2 receptor-binding domain (RBD); and one or both of: one or more conserved coronavirus CD4+ T cell target epitopes; one or more conserved coronavirus CD8+ T cell target epitopes; wherein at least one epitope is derived from a non-spike protein.

The present invention also features a pan-coronavirus recombinant vaccine composition, the composition comprising an antigen delivery system encoding whole spike protein; and one or more conserved coronavirus CD4+ T cell target epitopes; and one or more conserved coronavirus CD8+ T cell target epitopes; wherein at least one epitope is derived from a non-spike protein.

The present invention also features a pan-coronavirus recombinant vaccine composition, the composition comprising an antigen delivery system encoding at least a portion of spike protein, the portion of spike protein comprising a trimerized SARS-CoV-2 receptor-binding domain (RBD); and one or more conserved coronavirus CD4+ T cell target epitopes, and one or more conserved coronavirus CD8+ T cell target epitopes; wherein at least one epitope is derived from a non-spike protein.

Referring to the aforementioned compositions and the embodiments herein, in some embodiments, the non-spike protein is ORF1ab protein, ORF3a protein, Envelope protein, Membrane glycoprotein, ORF6 protein, ORF7a protein, ORF7b protein, ORF8 protein, Nucleocapsid protein and ORF10 protein.

In some embodiments, the one or more large sequences are highly conserved among human and animal coronaviruses. In some embodiments, the one or more large sequences are derived from at least one of SARS-CoV-2 protein. In some embodiments, the one or more large sequences are derived from one or more of: one or more SARS-CoV-2 human strains or variants in current circulation; one or more coronaviruses that has caused a previous human outbreak; one or more coronaviruses isolated from animals selected from a group consisting of bats, pangolins, civet cats, minks, camels, and other animal receptive to coronaviruses; or one or more coronaviruses that cause the common cold. In some embodiments, the one or more SARS-CoV-2 human strains or variants in current circulation are selected from: variant B.1.177; variant B.1.160, variant B.1.1.7 (UK), variant P.1 (Japan % Brazil), variant B.1.351 (South Africa), variant B.1.427 (California), variant B.1.429 (California), variant B.1.258; variant 8.1.221; variant B.1.367; variant 8.1.1.277; variant 8.1.1.302; variant B.1.525; variant B.1.526, variant S:677H; variant S:677P; B.1.617.2-Delta, variant B.1.1.529-Omicron (BA.1); sub-variant Omicron (BA.1); sub-variant Omicron (BA.2); sub-variant Omicron (BA.3); sub-variant Omicron (BA.4); sub-variant Omicron (BA.5). In some embodiments, the one or more coronaviruses that cause the common cold are selected from: 229E alpha coronavirus, NL63 alpha coronavirus, OC43 beta coronavirus, and HKU1 beta coronavirus. In some embodiments, the conserved large sequences are selected from Variants Of Concern or Variants Of Interest.

In some embodiments, the composition comprises two or more large sequences. In some embodiments, the composition comprises three or more large sequences. In some embodiments, the composition comprises two large sequences. In some embodiments, the composition comprises three large sequences. In some embodiments, the composition comprises four large sequences. In some embodiments, the composition comprises five large sequences.

In some embodiments, the large sequences are derived from structural proteins, non-structural proteins, or a combination thereof. In some embodiments, the large sequences or target epitopes are derived from a SARS-CoV-2 protein selected from a group consisting of: ORF1ab protein, Spike glycoprotein, ORF3a protein, Envelope protein, Membrane glycoprotein, ORF6 protein, ORF7a protein, ORF7b protein, ORF8 protein, Nucleocapsid protein an ORF10 protein.

In some embodiments, the large sequence or the target epitope derived from the Spike glycoprotein is RBD. In some embodiments, the large sequence or the target epitope derived from the Spike glycoprotein is NTD. In some embodiments, the large sequence or the target epitope derived from the Spike glycoprotein includes both the RBD and NTD regions. In some embodiments, the large sequence or the target epitope derived from the spike glycoprotein are recognized by neutralizing and blocking antibodies.

In some embodiments, the large sequence or the target epitope derived from the spike glycoprotein induces neutralizing and blocking antibodies. In some embodiments, the large sequence or the target epitope derived from the spike glycoprotein induces neutralizing and blocking antibodies that recognize and neutralize the virus.

In some embodiments, the large sequence or the target epitope derived from the spike glycoprotein induces neutralizing and blocking antibodies that recognize the spike protein.

In some embodiments, the ORF1ab protein comprises nonstructural protein (Nsp) 1, Nsp2, Nsp3, Nsp4, Nsp5, Nsp6, Nsp7, Nsp3, Nsp9, Nsp10, Nsp11, Nsp12, Nsp13, Nsp14, Nsp15 and Nsp16. In some embodiments, the one or more conserved coronavirus CD8+ T cell target epitopes are selected from: spike glycoprotein, Envelope protein, ORF1ab protein, ORF7a protein, ORF8a protein, ORF10 protein, or a combination thereof. In some embodiments, the one or more conserved coronavirus CD8+ T cell target epitopes are selected from: S_2-10, S_1225-1228, S_1000-1008, S_958-966, E_20-28, ORF1ab_1678-1683, ORF1ab_2363-2371, ORF1ab_3013-3021, ORF1ab_3183-3191, ORF1ab_5470-5478, ORF1ab_6749-6757, ORF7b_26-34, ORF8a_73-81, ORF1_03-11, and ORF1s-13. In some embodiments, the one or more conserved coronavirus CD8+ T cell target epitopes are selected from SEQ ID NO: 2-29 or SEQ ID NO: 194-213. In some embodiments, the one or more conserved coronavirus CD8+ T cell target epitopes are selected from SEQ ID NO: 30-57 or SEQ ID NO: 214-234. In some embodiments, the one or more conserved coronavirus CD4+ T cell target epitopes are selected from: spike glycoprotein, Envelope protein, Membrane protein, Nucleocapsid protein, ORF1a protein, ORF1ab protein, ORF6 protein, ORF7a protein, ORF7b protein, ORF8 protein, or a combination thereof. In some embodiments, the one or more conserved coronavirus CD4-+ T cell target epitopes are selected from: ORF1a_1350-1365, ORF1ab_5019-5033, ORF6_12-28, ORF1ab_6088-6102, ORF1ab_6420-6434, ORF1a_1801-1815, S_1-13, E_26-40, E_20-34, M_178-190, N_388-403, ORF7a_3-17, ORF7a_1-15, ORF7b_8-22, ORF7a_98-112, and ORF8_1-15. In some embodiments, the one or more conserved coronavirus CD4+ T cell target epitopes are selected from SEQ ID NO: 58-73 or SEQ ID NO: 235-253. In some embodiments, the one or more conserved coronavirus CD4+ T cell target epitopes are selected from SEQ ID NO: 74-105 or SEQ ID NO: 254-272. In some embodiments, the one or more conserved coronavirus B cell target epitopes are selected from Spike glycoprotein. In some embodiments, the one or more conserved coronavirus B cell target epitopes are selected from: S_287-317, S_524-598, S_601-440, S_802-819, S_883-909, S_369-393, S_440-501, S_1133-1172, S_329-363, and S_13-37. In some embodiments, the one or more coronavirus B cell target epitopes are selected from SEQ ID NO: 106-116 or SEQ ID NO: 273 to 280. In some embodiments, the one or more coronavirus B cell target epitopes are selected from SEQ ID NO: 117-138 or SEQ ID NO: 281-294.

In some embodiments, the one or more conserved coronavirus B cell target epitopes are in the form of a large sequence. In some embodiments, the large sequence is full length spike glycoprotein. In some embodiments, the large sequence is a partial spike glycoprotein. In some embodiments, the spike glycoprotein has two consecutive proline substitutions at amino acid positions 986 and 987. In some embodiments, the spike glycoprotein has single amino acid substitutions at amino acid positions comprising Tyr-83 and Tyr-489, Gln-24 and Asn-487. In some embodiments, the transmembrane anchor of the spike protein has an intact S1-S2 cleavage site. In some embodiments, the spike protein is in its stabilized conformation. In some embodiments, the spike protein is stabilized with proline substitutions at amino acid positions 986 and 987 at the top of the central helix in the S2 subunit.

In some embodiments, the one or more large sequences are derived from a whole protein sequence expressed by SARS-CoV-2. In some embodiments, the one or more large sequences are derived from a partial protein sequence expressed by SARS-CoV-2. In some embodiments, the one or more large conserved sequences from the spike protein is from a full-length spike glycoprotein. In some embodiments, the one or more large conserved sequences from the spike protein is from a partial spike glycoprotein. In some embodiments, the one or more large sequences comprises Spike glycoprotein (5) or a portion thereof, Nucleoprotein or a portion thereof, Membrane protein or a portion thereof, and ORF1a/b or a portion thereof. In some embodiments, the one or more large sequences comprises Spike glycoprotein (S) or a portion thereof, Nucleoprotein or a portion thereof, and ORF1a/b or a portion thereof. In some embodiments, the portion of the Spike glycoprotein is RBD. In some embodiments, the one or more large sequences is selected from the group consisting of: ORF1ab protein, Spike glycoprotein, ORF3a protein, Envelope protein, Membrane glycoprotein, ORF6 protein, ORF7a protein, ORF7b protein, ORF8 protein, Nucleocapsid protein an ORF10 protein. In some embodiments, the ORF1ab protein comprises nonstructural protein (Nsp) 1, Nsp2, Nsp3, Nsp4, Nsp5, Nsp6, Nsp7, Nsp8, Nsp9, Nsp10, Nsp11, Nsp12, Nsp13, Nsp14, Nsp15 and Nsp16. In some embodiments, one or more of the large sequences comprises a T-cell epitope restricted to a large number of human class 1 and class 2 HLA haplotypes and are not restricted to HLA-0201 for class 1 or HLA-DR for class 2.

In some embodiments, the large sequences are derived from structural proteins, non-structural proteins, or a combination thereof.

The present invention also features a recombinant vaccine composition comprising full-length spike protein. The present invention also features a recombinant vaccine composition comprising full-length spike protein or partial spike protein.

In some embodiments, the spike protein comprises Tyr-489 and Asn-487. In some embodiments, Tyr-489 and Asn-487 help with interaction with Tyr 83 and Gln-24 on ACE-2. In some embodiments, the spike protein comprises Gln-493. In some embodiments, Gln-493 helps with interaction with Glu-35 and Lys-31 on ACE-2. In some embodiments, the spike protein comprises Tyr-505. In some embodiments, Tyr-505 helps with interaction with Glu-37 and Arg-393 on ACE-2.

In some embodiments, the composition comprises a trimerized SARS-CoV-2 receptor-binding domain (RBD) sequence. In some embodiments, the trimerized SARS-CoV-2 receptor-binding domain (RBD) sequence is modified by the addition of a T4 fibritin-derived foldon trimerization domain. In some embodiments, the addition of a T4 fibritin-derived foldon trimerization domain increases immunogenicity by multivalent display. In some embodiments, the composition encodes the trimerized SARS-CoV-2 spike glycoprotein RBD antigen together with the one or more highly conserved structural and non-structural SARS-CoV-2 antigens. In some embodiments, the sequence for the antigen is GenBank accession number, MN908947.3. In some embodiments, the conserved large sequences are selected from the Variants Of Concern and Variants Of Interest. In some embodiments, the composition comprises a mutation 682-RRAR-685→682-QQAQ-685 in the S1-S2 cleavage site.

In some embodiments, the composition comprises at least one proline substitution. In some embodiments, the composition comprises at least two proline substitutions. In some embodiments, the proline substitution is at position K986 and V987. In some embodiments, the composition comprises K986P and V987P mutations.

In some embodiments, the large sequences are selected from SEQ ID NO. 182-185 (Table 1) or SEQ ID NO: 148-159 or SEQ ID NO: 348-359 (Table 10).

In some embodiments, the composition further comprises a pharmaceutical carrier. [0044] in some embodiments, the linker comprises T2A. In some embodiments, the linker is selected from T2A, E2A, and P2A. In some embodiments, a different linker is disposed between each open reading frame. [0045] in some embodiments, the vaccine constructs are for humans. In some embodiments, the composition comprises human CXCL-11 and IL-7 or IL-2 or IL-15. In some embodiments, the vaccine constructs are for animals. In some embodiments, the composition comprises animal CXCL-11 and IL-7 or IL-2 or IL-15. In some embodiments, the animals are cats and dogs.

In some embodiments, the delivery system is an adenovirus system. In some embodiments, the adenovirus delivery system is Ad26, Ad5, Ad35, or a combination thereof. In some embodiments, one or more of the large sequences are operatively linked to a generic promoter. In some embodiments, the generic promoter is a CMV or a CAG promoter. In some embodiments, the one or more large sequences are operatively linked to a lung-specific promoter. In some embodiments, the lung-specific promoter is SpB or CD144. In some embodiments, the composition further comprises a T cell attracting chemokine.

In some embodiments, the antigen delivery system further encodes a T cell attracting chemokine.

In some embodiments, the antigen delivery system comprises two delivery systems, wherein a second delivery system encodes the T cell attracting chemokine. In some embodiments, the T cell attracting chemokine is CCL5, CXCL9, CXCL10, CXCL11, or a combination thereof. In some embodiments, the T cell attracting chemokine is operatively linked to a lung-specific promoter. In some embodiments, the T cell attracting chemokine is operatively linked to a generic promoter. In some embodiments, the composition further comprises a composition that promotes T cell proliferation.

In some embodiments, the antigen delivery system further encodes a composition that promotes T cell proliferation. In some embodiments, the antigen delivery system comprises two delivery systems, wherein a second delivery system encodes the composition that promotes T cell proliferation. In some embodiments, the composition that promotes T cell proliferation is IL-7, IL-2, or IL-15. In some embodiments, the composition that promotes T cell proliferation is operatively linked to a lung-specific promoter. In some embodiments, the composition that promotes T cell proliferation is operatively linked to a generic promoter. In some embodiments, the T cell attracting chemokine and the composition that promotes T cell proliferation are driven by the same promoter. In some embodiments, the vaccine further encodes a peptide comprising a T cell attracting chemokine and a composition that promotes T cell proliferation. In some embodiments, the peptide is operatively linked to a lung-specific promoter. In some embodiments, the peptide is operatively linked to a generic promoter. In some embodiments, the lung-specific promoter is SpB or CD144. In some embodiments, the generic promoter is a CMV or a CAG promoter.

In some embodiments, the antigen delivery system further encodes a molecular adjuvant. In some embodiments, the antigen delivery system comprises two delivery systems, wherein a second delivery system encodes the molecular adjuvant. In some embodiments, the molecular adjuvant is CpG. In some embodiments, the molecular adjuvant is a CpG polymer. In some embodiments, the molecular adjuvant is flagellin. In some embodiments, the molecular adjuvant is operatively linked to a promoter. In some embodiments, the promoter is a lung-specific promoter or a generic promoter.

In some embodiments, one or more of the large sequences are separated by a linker. In some embodiments, each of the large sequences are separated by a linker. In some embodiments, the linker is from 2 to 10 amino acids in length.

In some embodiments, the recombinant vaccine composition comprises a tag, e.g., one or more of the large sequences comprises a tag. In some embodiments, the tag is a His tag.

The present invention also includes a rVSV-panCoV recombinant vaccine composition comprising any of the vaccine compositions herein.

The present invention also includes a rAdV-panCoV recombinant vaccine composition comprising any of the vaccine compositions herein.

In some embodiments, the compositions are for use as a vaccine. In some embodiments, the compositions are for use as immunotherapy for the prevention and treatment of Coronaviruses infections and diseases. In some embodiments, the composition is used to prevent a coronavirus disease in a subject.

In some embodiments, the composition is used to prevent a coronavirus infection prophylactically in a subject. In some embodiments, the composition elicits an immune response in a subject. In some embodiments, the composition prolongs an immune response induced by the pan-coronavirus recombinant vaccine composition and increases T-cell migration to the lungs.

The present invention also includes a pan-coronavirus recombinant vaccine composition comprising SEQ ID NO: 139-147 (Table 10).

Non-spike proteins include any of the coronavirus proteins other than spike, such as but not limited to Envelope protein, Membrane protein, Nucleocapsid protein, ORF1a protein, ORF1ab protein, ORF6 protein, ORF7a protein, ORF7b protein, ORF8 protein, etc.

In certain embodiments, the compositions of the present invention, e.g., the large sequences, comprise one or more conserved target epitopes, e.g.; one or more conserved coronavirus B-cell target epitopes; one or more conserved coronavirus CD4+ T cell target epitopes; and/or one or more conserved coronavirus CD8+ T cell target epitopes. In some embodiments, a conserved target epitope is one that is one of the 5 most conserved epitopes (for its epitope type, e.g., B cell, CD4 T cell, CD8 T cell) identified in a sequence alignment and analysis. In some embodiments, a conserved target epitope is one that is one of the 10 most conserved epitopes (for its epitope type, e.g., B cell, CD4 T cell, CD8 T cell) identified in a sequence alignment and analysis. In some embodiments, a conserved target epitope is one that is one of the 15 most conserved epitopes (for its epitope type, e.g., B cell. CD4 T cell, CD8 T cell) identified in a sequence alignment and analysis. In some embodiments, a conserved target epitope is one that is one of the 20 most conserved epitopes (for its epitope type, e.g., B cell, CD4 T cell. CD8 T cell) identified in a sequence alignment and analysis. In some embodiments, a conserved target epitope is one that is one of the 25 most conserved epitopes (for its epitope type, e.g., B cell, CD4 T cell, CD8 T cell) identified in a sequence alignment and analysis. In some embodiments, a conserved target epitope is one that is one of the 30 most conserved epitopes (for its epitope type, e.g. B cell, CD4 T cell, CD8 T cell) identified in a sequence alignment and analysis. In some embodiments, a conserved target epitope is one that is one of the 35 most conserved epitopes (for its epitope type, e.g. B cell, CD4 T cell, CD8 T cell) identified in a sequence alignment and analysis. In some embodiments, a conserved target epitope is one that is one of the 40 most conserved epitopes (for its epitope type, e.g., B cell, CD4 T cell, CD8 T cell) identified in a sequence alignment and analysis. In some embodiments, a conserved target epitope is one that is one of the 50 most conserved epitopes (for its epitope type, e.g., B cell, CD4 T cell, CD8 T cell) identified in a sequence alignment and analysis. Examples of sequence alignments and analyses. Are described herein. For example, steps or methods for selecting or identifying conserved large sequences may first include performing a sequence alignment and analysis of a particular number of coronavirus sequences to determine sequence similarity or identity amongst the group of analyzed sequences. In some embodiments, the sequences used for alignments may include human and animal sequences. In certain embodiments, the sequences used for alignments include one or more SARS-CoV-2 human strains or variants in current circulation; one or more coronaviruses that has caused a previous human outbreak; one or more coronaviruses isolated from animals selected from a group consisting of bats, pangolins, civet cats, minks, camels, and other animal receptive to coronaviruses; and/or one or more coronaviruses that cause the common cold. In some embodiments, the conserved large sequences are identified by: performing a sequence alignment and analysis of a particular number of coronavirus sequences to determine sequence similarity or identity amongst the group of analyzed sequences. The conserved large sequences are those that are among the most highly conserved sequences identified in the analysis. For example, the conserved large sequences may be the 2 most highly conserved sequences identified. In some embodiments, the conserved large sequences may be the 5 most highly conserved sequences identified. In some embodiments, the conserved large sequences may be the 8 most highly conserved sequences identified. In some embodiments, the conserved large sequences may be the 10 most highly conserved sequences identified. In some embodiments, the conserved large sequences may be the 15 most highly conserved sequences identified. In some embodiments, the conserved large sequences may be the 20 most highly conserved sequences identified. In some embodiments, the conserved large sequences may be the 30 most highly conserved sequences identified. In some embodiments, the conserved large sequences may be the 40 most highly conserved sequences identified. The present invention is not limited to the aforementioned thresholds. In some embodiments, the alignment and analysis for 50 or more sequences, 100 or more sequences, 200 or more sequences, 300 or more sequences, 400 or more sequences, 500 or more sequences, 1000 or more sequences, 2000 or more sequences, 3000 or more sequences, 4000 or more sequences, 5000 or more sequences, 10,000 or more sequences, 15,000 or more sequences, more than 15,000 sequences, etc., In some embodiments, the sequences used for alignments may include human and animal sequences. In certain embodiments, the sequences used for alignments include one or more SARS-CoV-2 human strains or variants in current circulation; one or more coronaviruses that has caused a previous human outbreak; one or more coronaviruses isolated from animals selected from a group consisting of bats, pangolins, civet cats, minks, camels, and other animal receptive to coronaviruses; and/or one or more coronaviruses that cause the common cold. In some embodiments, the one or more SARS-CoV-2 human strains or variants in current circulation are selected from: variant B.1.177; variant B.1.160, variant B.1.1.7 (UK), variant P.1 (Japan/Brazil), variant B.1.351 (South Africa), variant B.1.427 (California), variant B.1.429 (California), variant B.1.258; variant B.1.221; variant B.1.367; variant B.1.1.277; variant B.1.1.302; variant B.1.525; variant B.1.526, variant S:677H; variant S:677P: B.1.617.2-Delta, variant B.1.1.529-Omicron (BA.1); sub-variant Omicron (BA.1); sub-variant Omicron (BA.2); sub-variant Omicron (BA.3); sub-variant Omicron (BA.4); sub-variant Omicron (BA.5Y As discussed herein, the one or more conserved large sequences comprising target epitopes, are highly conserved among human and animal coronaviruses. For any of the embodiments herein, the epitopes that are selected may be those that achieve a particular score in a binding assay (for binding to an HLA molecule, for example.)

In certain embodiments, the one or more conserved coronavirus CD8+ T cell target epitopes are selected from: spike glycoprotein, Envelope protein, ORF1ab protein, ORF7a protein, ORF8a protein, ORF10 protein, or a combination thereof. In certain embodiments, the one or more conserved coronavirus CD8+ T cell target epitopes are selected from: S_2-10, S_1220-1228, S_1000-1008, S_958-966, E_20-28, ORF1ab_1675-1683, ORF1ab_2366-2371, ORF1ab_3013-3021, ORF1ab_3183-3191, ORF1ab_5470-5478, ORF1ab_6749-6757, ORF7b_28-34, ORF8a_73-81, ORF1_03-11, and ORF1_05-13. In certain embodiments, the one or more conserved coronavirus CD8+ T cell target epitopes are selected from SEQ ID NO: 2-29. In certain embodiments, the one or more conserved coronavirus CD8+ T cell target epitopes are selected from SEQ ID NO: 30-57.

In certain embodiments, the one or more conserved coronavirus CD4+ T cell target epitopes are selected from: spike glycoprotein, Envelope protein, Membrane protein, Nucleocapsid protein, ORF1a protein, ORF1ab protein, ORF6 protein, ORF7a protein, ORF7b protein, ORF8 protein, or a combination thereof. In certain embodiments, the one or more conserved coronavirus CD4+ T cell target epitopes are selected from: ORF1a_1350-1385, ORF1ab_5019-5033, ORF6_12-28s ORF1ab_6088-6102, ORF1ab_6420-6434, ORF1a_1801-1815, S_1-13, E_26-40, E_20-34, M_176-190, N_388-403, ORF7a_3-17, ORF7a_1-15, ORF7b_8-22, ORF7a_98-112, and ORF8_1-15. In certain embodiments, the one or more conserved coronavirus CD4+ T cell target epitopes are selected from SEQ ID NO: 58-73. In certain embodiments, the one or more conserved coronavirus CD4+ T cell target epitopes are selected from SEQ ID NO: 74-105.

In certain embodiments, the one or more conserved coronavirus B cell target epitopes are selected from Spike glycoprotein. In certain embodiments, the one or more conserved coronavirus B cell target epitopes are selected from: S_287-317, S_524-598, S_601-640, S_802-819, S_888-909, S_369-393, S_440-501, S_1133-1172, S_329-363, and S_13-37. In certain embodiments, the one or more coronavirus B cell target epitopes are selected from SEQ ID NO: 106-116 in certain embodiments, the one or more coronavirus B cell target epitopes are selected from SEQ ID NO: 117-138.

As previously discussed, in certain embodiments, the one or more conserved coronavirus B cell target epitopes are in the form of a large sequence, e.g., whole spike protein or partial spike protein (e.g., a portion of whole spike protein). In some embodiments, the whole spike protein or portion thereof is in its stabilized conformation. In certain embodiments, the transmembrane anchor of the spike protein (or portion thereof) has an intact S1-S2 cleavage site. In certain embodiments, the spike glycoprotein has two consecutive proline substitutions at amino acid positions 986 and 987, e.g., for stabilization. In certain embodiments, the spike protein or portion thereof has an amino acid substitution at amino acid position Tyr-83. In certain embodiments, the spike protein or portion thereof has an amino acid substitution at amino acid position Tyr-489. In certain embodiments, the spike protein or portion thereof has an amino acid substitution at amino acid position Gln-24. In certain embodiments, the spike protein or portion thereof has an amino acid substitution at amino acid position Asn-487. In certain embodiments, the spike protein or portion thereof has an amino acid substitution at one or more of: Tyr-83, Tyr-489, Gln-24, Gln-493, and Asn-487, e.g., the spike protein or portion thereof may comprise Tyr-489 and Asn-487, the spike protein or portion thereof may comprise Gln-493, the spike protein or portion thereof may comprise Tyr-505, etc. Tyr-489 and Asn-487 may help with interaction with Tyr 83 and Gln-24 on ACE-2. Gln-493 may help with interaction with Glu-35 and Lys-31 on ACE-2. Tyr-505 may help with interaction with Glu-37 and Arg-393 on ACE-2.

In certain embodiments, the composition comprises a mutation 682-RRAR-685→682-QQAQ-685 in the S1-32 cleavage site. In certain embodiments, the composition comprises at least one proline substitution. In certain embodiments, the composition comprises at least two proline substitutions, e.g., at position K986 and V987.

In certain embodiments, a large sequence derived from the spike glycoprotein is RBD. In certain embodiments, a large sequence derived from the spike glycoprotein is NTD. In certain embodiments, a large sequence derived from the spike glycoprotein is one or more large sequences, e.g., comprising both the RBD and NTD regions. In certain embodiments, a large sequence derived from the spike glycoprotein is recognized by neutralizing and blocking antibodies. In certain embodiments, a large sequence derived from the spike glycoprotein induces neutralizing and blocking antibodies. In certain embodiments, a large sequence derived from the spike glycoprotein induces neutralizing and blocking antibodies that recognize and neutralize the virus. In certain embodiments, a large sequence derived from the spike glycoprotein induces neutralizing and blocking antibodies that recognize the spike protein.

In certain embodiments, linkers are used, e.g., between epitopes, between large sequences, etc. In certain embodiments, the linker is from 2-10 amino acids in length. In certain embodiments, the linker is from 3-12 amino acids in length. In certain embodiments, the linker is from 5-15 amino acids in length. In certain embodiments, the linker is 10 or more amino acids in length. Non-limiting examples of linkers include AAY, KK, and GPGPG.

In some embodiments, the composition comprises the addition of a T4 fibritin-derived foldon trimerization domain. In some embodiments, the addition of a T4 fibritin-derived foldon trimerization domain increases immunogenicity by multivalent display. [0066] in certain embodiments, the composition further comprises a T cell attracting chemokine. For example, the composition may further comprise one or a combination of CCL5, CXCL9, CXCL10, CXCL11, or a combination thereof.

In certain embodiments, the composition further comprises a composition that promotes T cell proliferation. For example, the composition may further comprise IL-7, IL-15, IL-2, or a combination thereof.

In certain embodiments, the composition further comprises a molecular adjuvant. For example, the composition may further comprise one or a combination of CpG (e.g., CpG polymer) or flagellin.

In certain embodiments, the composition comprises a tag. For example, one or more of the large sequences may comprise a tag. In certain embodiments, the epitopes are in the form of two or more antigens, wherein one or more of the antigens comprise a tag. Non-limiting examples of tags include a His tag.

In certain embodiments, the “antigen delivery system” may refer to two delivery systems, e.g., a portion of the large sequences (or other components such as chemokines, etc.) may be encoded by one delivery system and a portion of the large sequences (or other components) may be encoded by a second delivery system (or a third delivery system, etc.).

Referring to the antigen delivery system, in certain embodiments the antigen delivery system is a vesicular stomatitis virus (VSV) vector. In certain embodiments, the antigen delivery system is an adenovirus (e.g., Ad26, Ad5, Ad35, etc.)

The large sequences are operatively linked to a promoter. In certain embodiments, the promoter is a generic promoter (e.g., CMV, CAG, etc.). In certain embodiments, the promoter is a lung-specific promoter (e.g., SpB, CD144). In certain embodiments, large sequences are operatively linked to the same promoter. In certain embodiments, one or more of the large sequences are operatively linked to a first promoter and one or more large sequences are operatively linked to a second promoter. In certain embodiments, the large sequences are operatively linked to two or more promoters, e.g., a portion are operatively linked to a first promoter, a portion are operatively linked to a second promoter, etc. In certain embodiments, the large sequences are operatively linked to three or more promoters, e.g., a portion is operatively linked to a first promoter, a portion is operatively linked to a second promoter, a portion is operatively linked to a third promoter, etc. In certain embodiments, the first promoter is the same as the second promoter. In certain embodiments the second promoter is different from the first promoter. In certain embodiments, the promoter is a generic promoter (e.g., CMV, CAG, etc.). In certain embodiments, the promoter is a lung-specific promoter (e.g., SpB, CD144) promoter.

In certain embodiments, the antigen delivery system or a separate antigen delivery system encodes a T cell attracting chemokine. In certain embodiments, the antigen delivery system or a separate antigen delivery system encodes a composition that promotes T cell proliferation. In certain embodiments, the antigen delivery system or a separate antigen delivery system encodes both a T cell attracting chemokine and a composition that promotes T cell proliferation. In certain embodiments, the antigen delivery system or a separate antigen delivery system encodes a molecular adjuvant. In certain embodiments, the antigen delivery system or a separate antigen delivery system encodes a T cell attracting chemokine, a composition that promotes T cell proliferation and a molecular adjuvant. In certain embodiments, the antigen delivery system or a separate antigen delivery system encodes a T cell attracting chemokine and a molecular adjuvant. In some embodiments, the antigen delivery system or a separate antigen delivery system encodes a composition that promotes T cell proliferation and a molecular adjuvant.

In certain embodiments, the T cell attracting chemokine is CCL5, CXCL9, CXCL10, CXCL11, or a combination thereof. In certain embodiments, the composition that promotes T cell proliferation is IL-7 or IL-15 or IL-2. In some embodiments, the molecular adjuvant is CpG (e.g., CpG polymer), flagellin, etc.).

In certain embodiments, the T cell attracting chemokine is operatively linked to a lung-specific promoter (e.g., SpB, CD144). In certain embodiments, the T cell attracting chemokine is operatively linked to a generic promoter (e.g., CMV, CAG, etc.). In certain embodiments, the composition that promotes T cell proliferation is operatively linked to a lung-specific promoter (e.g., SpB, CD144). In certain embodiments, the composition that promotes T cell proliferation is operatively linked to a generic promoter (e.g., CMV, CAG, etc.). In certain embodiments, the molecular adjuvant is operatively linked to a lung-specific promoter (e.g., SpB, CD144). In certain embodiments, the molecular adjuvant is operatively linked to a generic promoter (e.g., CMV, CAG, etc.). In certain embodiments, the T cell attracting chemokine and the composition that promotes T cell proliferation are driven by the same promoter. In certain embodiments, the T cell attracting chemokine and the composition that promotes T cell proliferation are driven by different promoters. In certain embodiments, the molecular adjuvant, the T cell attracting chemokine, and the composition that promotes T cell proliferation are driven by the same promoter. In certain embodiments, the molecular adjuvant, the T cell attracting chemokine, and the composition that promotes T cell proliferation are driven by different promoters. In certain embodiments, the molecular adjuvant and the composition that promotes T cell proliferation are driven by different promoters. In certain embodiments, the molecular adjuvant and the T cell attracting chemokine are driven by different promoters.

In certain embodiments, the T cell attracting chemokine and the composition promoting T cell proliferation are separated by a linker, in certain embodiments, the linker comprises T2A. In certain embodiments, the linker comprises E2A. In certain embodiments, the linker comprises P2A. In certain embodiments, the linker is selected from T2A, E2A, and P2A.

Referring to the antigen delivery system, in certain embodiments, a linker is disposed between each open reading frame. In certain embodiments, a different linker is disposed between each open reading frame. In certain embodiments, the same linker may be used between particular open reading frames and a different linker may be used between other open reading frames.

In some embodiments, the vaccine composition is administered using an adenovirus.

The composition herein may be used to prevent a coronavirus disease in a subject. The composition herein may be used to prevent a coronavirus infection prophylactically in a subject. The composition herein may be used to elicit an immune response in a subject. The term “subject” herein may refer to a human, a non-human primate, an animal such as a mouse, rat, cat, dog, other animal that is susceptible to coronavirus infection, or other animal used for preclinical modeling. The composition herein may prolong an immune response induced by the pan-coronavirus recombinant vaccine composition and increases T-cell migration to the lungs. In certain embodiments, the composition induces resident memory T cells (Trm). In some embodiments, the vaccine composition induces efficient and powerful protection against the coronavirus disease or infection. In some embodiments, the vaccine composition induces production of antibodies (Abs), CD4+ T helper (Th1) cells, and CD8+ cytotoxic T-cells (CTL). In some embodiments, the composition that promotes T cell proliferation helps to promote long term immunity. In some embodiments, the T-cell attracting chemokine helps pull T-cells from circulation into the lungs.

In certain embodiments, the composition further comprises a pharmaceutical carrier.

The present invention includes any of the vaccine compositions described herein, e.g., the aforementioned vaccine compositions for delivery with nanoparticles, e.g., lipid nanoparticles. For example, the present invention includes the vaccine compositions herein encapsulated in a lipid nanoparticle.

The present invention includes the compositions described herein comprising and/or encoding a trimerized SARS-CoV-2 receptor-binding domain (RBD) and one or more highly conserved SARS-CoV-2 sequences selected from structural proteins (e.g., nucleoprotein, etc.) and non-structural protein (e.g., Nsp4, etc.). In some embodiments, the trimerized SARS-CoV-2 receptor-binding domain (RBD) sequence is modified by the addition of a T4 fibritin-derived foldon trimerization domain. In some embodiments, the addition of a T4 fibritin-derived foldon trimerization domain increases immunogenicity by multivalent display.

The present invention also features methods of producing a pan-coronavirus recombinant vaccine compositions of the present invention.

For example, in some embodiments, the method comprises selecting at least conserved large sequences comprising: one or more coronavirus B-cell epitopes; one or more coronavirus CD4+ T cell epitopes; one or more coronavirus CD8+ T cell epitopes. In other embodiments, the method comprises selecting at least two conserved large sequences comprising: one or more coronavirus B-cell epitopes; one or more coronavirus CD4-+ T cell epitopes; one or more coronavirus CD8+ T cell epitopes. At least one large sequence is derived from a non-spike protein. The method further comprises synthesizing an antigen or antigens comprising the selected large sequences. In some embodiments, the method comprises selecting: one or more conserved large sequences comprising one or more coronavirus B-cell epitopes; one or more coronavirus CD4+ T cell epitopes; and one or more coronavirus CD8+ T cell epitopes. At least one large sequence is derived from a non-spike protein. The method further comprises synthesizing an antigen or antigens comprising the selected large sequences. In some embodiments, the method further comprises introducing the vaccine composition to a pharmaceutical carrier. The steps for selecting the one or more conserved large sequences are disclosed herein. Methods for synthesizing recombinant proteins are well known to one of ordinary skill in the art. The vaccine compositions are disclosed herein. In some embodiments, the vaccine composition is in the form of DNA, RNA, modified RNA, protein (or peptide), or a combination thereof.

In some embodiments, the method comprises selecting: at least one conserved large sequence comprising: one or more coronavirus B-cell epitopes; one or more coronavirus CD4+ T cell epitopes; and one or more coronavirus CD8+ T cell epitopes. At least one large sequence is derived from a non-spike protein. The method further comprises synthesizing an antigen delivery system encoding the selected large sequences. In some embodiments, the method further comprises introducing the vaccine composition to a pharmaceutical carrier. The steps for selecting the one or more conserved large sequences are disclosed herein. Methods for synthesizing antigen delivery systems are well known to one of ordinary skill in the art. The vaccine compositions are disclosed herein. In some embodiments, the vaccine composition is in the form of DNA, RNA, modified RNA, protein (or peptide), or a combination thereof.

As an example, steps or methods for selecting or identifying conserved large sequences may first include performing a sequence alignment and analysis of a particular number of coronavirus sequences, e.g., 50 or more sequences, 100 or more sequences, 200 or more sequences, 300 or more sequences, 400 or more sequences, 500 or more sequences, 1000 or more sequences, 2000 or more sequences, 3000 or more sequences, 4000 or more sequences, 5000 or more sequences, 10,000 or more sequences, 15,000 or more sequences, more than 15,000 sequences, etc., to determine sequence similarity or identity amongst the group of analyzed sequences. In some embodiments, the sequences used for alignments may include human and animal sequences. In certain embodiments, the sequences used for alignments include one or more SARS-CoV-2 human strains or variants in current circulation; one or more coronaviruses that has caused a previous human outbreak; one or more coronaviruses isolated from animals selected from a group consisting of bats, pangolins, civet cats, minks, camels, and other animal receptive to coronaviruses; and/or one or more coronaviruses that cause the common cold. In some embodiments, the one or more SARS-CoV-2 human strains or variants in current circulation are selected from: variant B.1.177; variant B.1.160, variant B.1.1.7 (UK), variant P.1 (Japan/Brazil), variant 8.1.351 (South Africa), variant B.1.427 (California), variant B.1.429 (California), variant B.1.258; variant B.1.221; variant B.1.367; variant B.1.1.277; variant B.1.1.302; variant B.1.525; variant B.1.526, variant S:677H; variant S:677P; B.1.617.2-Delta, variant B.1.1.529-Omicron (BA.1); sub-variant Omicron (BA.1); sub-variant Omicron (BA.2); sub-variant Omicron (BA.3); sub-variant Omicron (BA.4); sub-variant Omicron (BA.5). In some embodiments, the one or more coronaviruses that cause the common cold are selected from: 229E alpha coronavirus, NL63 alpha coronavirus, OC43 beta coronavirus, and HKU1 beta coronavirus. In some embodiments, the conserved large sequences may be considered the 2 most highly conserved sequences of the identified large sequences in the alignment. In some embodiments, the conserved large sequences may be considered the 5 most highly conserved sequences of the identified large sequences in the alignment. In some embodiments, the conserved large sequences may be considered the 10 most highly conserved sequences of the identified large sequences in the alignment. In some embodiments, the conserved large sequences may be considered the 15 most highly conserved sequences of the identified large sequences in the alignment.

The present invention also features methods for preventing coronavirus disease. The method comprises administering to a subject a therapeutically effective amount of a pan-coronavirus recombinant vaccine composition according to the present invention, wherein the composition elicits an immune response in the subject and helps prevent coronavirus disease.

The present invention also features methods for preventing a coronavirus infection prophylactically in a subject. In some embodiments, the method comprises administering to the subject a prophylactically effective amount of a pan-coronavirus recombinant vaccine composition according to the present invention, wherein the vaccine composition prevents coronavirus infection.

The present invention also features methods for eliciting an immune response in a subject, comprising administering to the subject a composition according to the present invention, wherein the vaccine composition elicits an immune response in the subject. The present invention also features methods comprising: administering to a subject a pan-coronavirus recombinant vaccine composition according to the present invention, wherein the composition prevents virus replication in the lungs, the brain, and other compartments where the virus replicates. The present invention also features methods comprising: administering to the subject a pan-coronavirus recombinant vaccine composition according to the present invention, wherein the composition prevents cytokine storm in the lungs, the brain, and other compartments where the virus replicates. The present invention also features methods comprising: administering to the subject a pan-coronavirus recombinant vaccine composition according to the present invention, wherein the composition prevents inflammation or inflammatory response in the lungs, the brain, and other compartments where the virus replicates. The present invention also features methods comprising: administering to the subject a pan-coronavirus recombinant vaccine composition according to the present invention, wherein the composition improves homing and retention of T cells in the lungs, the brain, and other compartments where the virus replicates. The present invention also features methods for preventing coronavirus disease in a subject; the method comprising: administering to the subject a pan-coronavirus recombinant vaccine composition according to the present invention, wherein the composition induces memory B and T cells. The present invention also features methods for prolonging an immune response induced by a pan-coronavirus recombinant vaccine and increasing T-cell migration to the lungs, the method comprising: co-expressing a T-cell attracting chemokine, a composition that promotes T cell proliferation, and a pan-coronavirus recombinant vaccine according to the present invention. The present invention also features methods for prolonging the retention of memory T-cell into the lungs induced by a pan coronavirus vaccine and increasing virus-specific tissue resident memory T-cells (TRM cells), the method comprising: co-expressing a T-cell attracting chemokine, a composition that promotes T cell proliferation, and a pan-coronavirus recombinant vaccine according to the present invention. The present invention also features methods comprising: administering to the subject a pan-coronavirus recombinant vaccine composition according to the present invention, wherein the composition prevents the development of mutation and variants of a coronavirus.

For the sake of brevity, it is noted that the vaccine compositions referred to in the aforementioned methods include the vaccine compositions previously discussed, the embodiments described below, and the embodiments in the figures.

In some embodiments, the vaccine composition is administered through an intravenous route (i.v.), an intranasal route (i.n.), or a sublingual route (s.l.) route.

In some embodiments, the vaccine composition is administered using an adenovirus or other appropriate delivery system.

As previously discussed, the composition herein may be used to prevent a coronavirus disease in a subject. The composition herein may be used to prevent a coronavirus infection prophylactically in a subject. The composition herein may be used to elicit an immune response in a subject. The term “subject” herein may refer to a human, a non-human primate, an animal such as a mouse, rat, cat, dog, other animal that is susceptible to coronavirus infection, or other animal used for preclinical modeling. The composition herein may prolong an immune response induced by the pan-coronavirus recombinant vaccine composition and increases T-cell migration to the lungs. In certain embodiments, the composition induces resident memory T cells (Trm). In some embodiments, the vaccine composition induces efficient and powerful protection against the coronavirus disease or infection. In some embodiments, the vaccine composition induces production of antibodies (Abs), CD4+ T helper (Th1) cells, and CD8+ cytotoxic T-cells (CTL). In some embodiments, the composition that promotes T cell proliferation helps to promote long term immunity. In some embodiments, the T-cell attracting chemokine helps pull T-cells from circulation into the lungs.

The present invention also features oligonucleotide compositions. For example, the present invention includes oligonucleotides disclosed in the sequence listings. The present invention also includes oligonucleotides in the form of antigen delivery systems. The present invention also includes oligonucleotides encoding the conserved large sequences disclosed herein. The present invention also includes oligonucleotide compositions comprising one or more oligonucleotides encoding any of the vaccine compositions according to the present invention. In some embodiments, the oligonucleotide comprises DNA. In some embodiments, the oligonucleotide comprises modified DNA. In some embodiments, the oligonucleotide comprises RNA. In some embodiments, the oligonucleotide comprises modified RNA. In some embodiments, the oligonucleotide comprises mRNA. In some embodiments, the oligonucleotide comprises modified mRNA.

The present invention also features peptide compositions. For example, the present invention includes peptides disclosed in the sequence listings. The present invention also includes peptide compositions comprising any of the vaccine compositions according to the present invention. The present invention also includes peptide compositions comprising any of the conserved large sequences according to the present invention.

For the sake of brevity, it is noted that the vaccine compositions referred to in the aforementioned oligonucleotide and peptide compositions include the vaccine compositions previously discussed, the embodiments described below, and the embodiments in the figures.

The present invention also features a pan-coronavirus recombinant vaccine composition comprising SEQ ID NO 139-147 (Table 9). The present invention also features a pan-coronavirus recombinant vaccine composition at least 99% identical to SEQ ID NO: 139-147 (Table 9).

The present invention also features a method comprising: administering a first pan-coronavirus recombinant vaccine dose using a first delivery system, and administering a second vaccine dose using a second delivery system, wherein the first and second delivery system are different. In some embodiments, the first delivery system may comprise a RNA, a modified mRNA, or a peptide delivery system. In some embodiments, the second delivery system may comprise a RNA, a modified mRNA, or a peptide delivery system. In some embodiments, the peptide delivery system is an adenovirus. In some embodiments, the adenovirus delivery system is Ad26, Ad5, Ad35, or a combination thereof. In some embodiments, the peptide delivery system is a vesicular stomatitis virus (VSV) vector. In some embodiments, the second vaccine dose is administered 14 days after the first vaccine dose.

The present invention also features a method comprising: administering a pan-coronavirus recombinant vaccine composition according to the present invention: and administering at least one T-cell attracting chemokine after administering the pan-coronavirus recombinant vaccine composition. In some embodiments, the vaccine composition is administered via a RNA, a modified mRNA, or a peptide delivery system. In some embodiments, the T-cell attracting chemokine is administered via a RNA, a modified mRNA, or a peptide delivery system. In some embodiments, the peptide delivery system is an adenovirus.

In some embodiments, the adenovirus delivery system is Ad26, Ad5, Ad35, or a combination thereof. In some embodiments, the peptide delivery system is a vesicular stomatitis virus (VSV) vector. In some embodiments, the T-cell attracting chemokine is administered 8 days after administering days after the vaccine composition. In some embodiments, the T-cell attracting chemokine is administered 14 days after administering days after the vaccine composition. In some embodiments, the T-cell attracting chemokine is administered 30 days after administering days after the vaccine composition. In some embodiments, the T-cell attracting chemokine is CCL5, CXCL9, CXCL10, CXCL11, or a combination thereof.

The present invention also features a method comprising: administering a pan-coronavirus recombinant vaccine composition according to the present invention; administering at least one T-cell attracting chemokine after administering the pan-coronavirus recombinant vaccine composition; and administering at least one cytokine after administering the T-cell attracting chemokine. In some embodiments, the vaccine composition is administered via a RNA, a modified mRNA, or a peptide delivery system. In some embodiments, the T-cell attracting chemokine is administered via a RNA, a modified mRNA, or a peptide delivery system. In some embodiments, the cytokine is administered via a RNA, a modified mRNA, or a peptide delivery system. In some embodiments, the peptide delivery system is an adenovirus. In some embodiments, the adenovirus delivery system is Ad26, Ad5, Ad35, or a combination thereof. In some embodiments, the peptide delivery system is a vesicular stomatitis virus (VSV) vector. In some embodiments, the T-cell attracting chemokine is administered 14 days after administering the vaccine composition. In some embodiments, the T-cell attracting chemokine is CCL5, CXCL9, CXCL10, CXCL11, or a combination thereof. In some embodiments, the cytokine is administered 10 days after administering the T-cell attracting chemokine. In some embodiments, the cytokine is IL-7, IL-15, IL2 or a combination thereof.

The present invention also features a method comprising: administering a pan-coronavirus recombinant vaccine composition according to the present invention; administering one or more T-cell attracting chemokine after administering the pan-coronavirus recombinant vaccine composition; and administering one or more mucosal chemokine(s). In some embodiments, the vaccine composition is administered using an adenovirus. In some embodiments, the T-cell attracting chemokine is administered via a RNA, a modified mRNA, or a peptide delivery system, or other delivery system. In some embodiments, the mucosal chemokine is administered via a RNA, a modified mRNA, or a peptide delivery system, or other delivery system. In some embodiments, the adenovirus is Ad26, Ad5, Ad35, or a combination thereof. In some embodiments, the T-cell attracting chemokine is administered 14 days after administering the vaccine composition. In some embodiments, the T-cell attracting chemokine is CCL5, CXCL9, CXCL10, CXCL11, or a combination thereof. In some embodiments, the mucosal chemokine is administered 10 days after administering the T-cell attracting chemokine. In some embodiments, the mucosal chemokine is CCL25, CCL28, CXCL14, or CXCL17, or a combination thereof.

As previously discussed, in some embodiments, the vaccine compositions are for use in humans. In some embodiments, the vaccine compositions are for use in animals, e.g., cats, dogs, etc. In some embodiments, the vaccine composition comprises human CXCL-11 and/or human IL-7 (or IL-15, IL-2). In some embodiments, the vaccine composition comprises animal CLCL-11 and/or animal IL-7 (or IL-15, IL-2).

The present invention includes vaccine compositions in the form of a rVSV-panCoV vaccine composition. The present invention includes vaccine compositions in the form of a rAdV-panCoV vaccine composition.

The present invention also includes nucleic acids for use in the vaccine compositions herein. The present invention also includes vectors for use in the vaccine compositions herein. The present invention also includes fusion proteins for use in the vaccine compositions herein. The present invention also includes immunogenic compositions for use in the vaccine compositions herein.

The vaccine compositions herein may be designed to elicit both high levels of virus-blocking and virus-neutralizing antibodies as well as CD4+ T cells and CD8+ T cells in adults 18 to 55 years. The vaccine compositions herein may be designed to elicit both high levels of virus-blocking and virus-neutralizing antibodies as well as CD4+ T cells and CD8+ T cells in adults 55 to 65 years of age. The vaccine compositions herein may be designed to elicit both high levels of virus-blocking and virus-neutralizing antibodies as well as CD4+ T cells and CD8+ T cells in adults 65 to 85 years of age. The vaccine compositions herein may be designed to elicit both high levels of virus-blocking and virus-neutralizing antibodies as well as CD4+ T cells and CD8+ T cells in adults 85 to 100 years of age. The vaccine compositions herein may be designed to elicit both high levels of virus-blocking and virus-neutralizing antibodies as well as CD4+ T cells and CD8+ T cells in children 12 to 18 years of age. The vaccine compositions herein may be designed to elicit both high levels of virus-blocking and virus-neutralizing antibodies as well as CD4+ T cells and CD8+ T cells in children under 12 years of age.

The present invention is not limited to vaccine compositions. For example, in certain embodiments, one or more of the conserved large sequences are used for detecting coronavirus and/or diagnosing coronavirus infection.

As previously discussed, in some embodiments, the one or more conserved large sequences are highly conserved among human and animal coronaviruses. In some embodiments, the conserved large sequence is one that is among the most highly conserved large sequences identified in a sequence alignment and analysis of a particular number of coronavirus sequences. For example, the conserved large sequence may be the 2 most highly conserved large sequences identified. In some embodiments, the conserved large sequences may be the 5 most highly conserved large sequences identified. In some embodiments, the conserved large sequences may be the 8 most highly conserved large sequences identified. In some embodiments, the conserved large sequences may be the 10 most highly conserved large sequences identified. In some embodiments, the conserved large sequences may be the 15 most highly conserved large sequences identified. In some embodiments, the conserved large sequences may be the 20 most highly conserved large sequences identified. In some embodiments, the conserved large sequences may be the 30 most highly conserved large sequences identified. In some embodiments, the conserved large sequences may be the 40 most highly conserved large sequences identified. In some embodiments, the one or more conserved. In some embodiments, the conserved large sequences may be the 5 most highly conserved large sequences identified are derived from at least one of SARS-CoV-2 protein. In some embodiments, the one or more conserved In some embodiments, the conserved large sequences may be the 5 most highly conserved large sequences identified are derived from one or more of: one or more SARS-CoV-2 human strains or variants in current circulation; one or more coronaviruses that has caused a previous human outbreak; one or more coronaviruses isolated from animals selected from a group consisting of bats, pangolins, civet cats, minks, camels, and other animal receptive to coronaviruses; or one or more coronaviruses that cause the common cold. In some embodiments, the one or more SARS-CoV-2 human strains or variants in current circulation are selected from variant B.1.177; variant B.1.160, variant B.1.1.7 (UK), variant P.1 (Japan/Brazil), variant B.1.351 (South Africa), variant B.1.427 (California), variant B.1.429 (California), variant B.1.258; variant B.1.221; variant 8.1.367; variant 8.1.1.277; variant B.1.1.302; variant B.1.525; variant B1.526, variant S:677H; variant S:677P; B.1.617.2-Delta, variant B.1.1.529-Omicron (BA.1); sub-variant Omicron (BA.1); sub-variant Omicron (BA.2); sub-variant Omicron (BA.3); sub-variant Omicron (BA.4); sub-variant Omicron (BA.5). In some embodiments, the one or more coronaviruses that cause the common cold are selected from: 229E alpha coronavirus, NL63 alpha coronavirus, OC43 beta coronavirus, and HKU1 beta coronavirus. In some embodiments, the vaccine composition is for humans. In some embodiments, the vaccine composition is for animals.

The present invention also features a method of producing a pan-coronavirus composition, the method comprising selecting at least one large sequence(s) according to the present invention and synthesizing one or more antigens comprising the selected large sequence(s). The present invention also features a method of producing a pan-coronavirus composition, the method comprising selecting at least one conserved large sequence(s); and synthesizing an antigen delivery system that encodes the selected large sequence(s).

The present invention also includes a pan-coronavirus recombinant vaccine composition, the composition comprising one or more large sequences, each of the one or more large sequences comprises at least one of: whole spike protein or a portion thereof; one or more conserved coronavirus CD4+ T cell target epitope; and one or more conserved coronavirus CD8+ T cell target epitope; wherein at least one epitope is derived from a non-spike protein.

In some embodiments, the one or more conserved epitopes are highly conserved among human and animal coronaviruses. In some embodiments, the one or more conserved epitopes are derived from at least one of SARS-CoV-2 protein. In some embodiments, the composition comprises 2-20 CD8+ T cell target epitopes. In some embodiments, the composition comprises 2-20 CD4+ T cell target epitopes. In some embodiments, the one or more conserved coronavirus CD4+ T cell target epitopes selected from SEQ ID NO: 58-105 (ORF1a1350-1365, ORF1ab5019-5033, ORF612-26, ORF1ab6088-6102, ORF1ab6420-6434, ORF1a1801-1815, S1-13, E26-40, E20-34, M176-190, N388-403, ORF7a3-17, ORF7a1-15, ORF7b8-22, ORF7a98-112, and ORF81-15.). In some embodiments, the one or more conserved coronavirus CD8+ T cell target epitopes selected from SEQ ID NO: 106-138 (S287-317, S524-598, S601-640, S802-819, S888-909, S369-393, S440-501, S1133-1172, S329-363, and S13-37).

The present invention also features a pan-coronavirus recombinant vaccine composition, the composition comprising one or more large sequences, each of the one or more large sequences comprises at least one of: one or more conserved coronavirus B-cell target epitope; one or more conserved coronavirus CD4+ T cell target epitope; and/or one or more conserved coronavirus CD8+ T cell target epitope, wherein at least one epitope is derived from a non-spike protein.

In some embodiments, the one or more conserved epitopes are derived from at least one of SARS-CoV-2 proteins. In some embodiments, the composition comprises 2-20 CD8+ T cell target epitopes.

In some embodiments, the composition comprises 2-20 CD4+ T cell target epitopes. In some embodiments, the one or more conserved coronavirus CD4+ T cell target epitopes selected from SEQ ID NO 58-105 (ORF1a1350-1365, ORF1ab5019-5033, ORF612-26, ORF1ab6088-6102, ORF1ab6420-6434, ORF1a1801-1815, S1-13, E26-40, E20-34, M176-190, N388-403, ORF7a3-17, ORF7a1-15, ORF7b8-22, ORF7a98-112, and ORF81-15.).

In some embodiments, the one or more conserved coronavirus CD8+ T cell target epitopes selected from SEQ ID NO: 106-138 (S287-317, 3524-598, S601-640, S802-819, 3888-909, S369-393, S440-501, S1133-1172, S329-363, and S13-37).

The present invention also features a pan-coronavirus recombinant vaccine composition, the composition comprising an antigen delivery system encoding one or more large sequences, the large sequences comprise at least one of: one or more conserved coronavirus B-cell target epitopes; one or more conserved coronavirus CD4+ T cell target epitopes; and/or one or more conserved coronavirus CD8+ T cell target epitopes; wherein at least one epitope is derived from a non-spike protein.

In some embodiments, the antigen delivery system is an adenovirus-based antigen delivery system. In some embodiments, the adenovirus-based antigen delivery system is Ad26, Ad5, Ad35, or a combination thereof. In some embodiments, the antigen delivery system further encodes a T cell attracting chemokine. In some embodiments, the antigen delivery system further encodes a composition that promotes T cell proliferation. In some embodiments, the antigen delivery system further encodes a molecular adjuvant. In some embodiments, the large sequences are operatively linked to a lung-specific promoter.

In some embodiments, the one or more conserved coronavirus B-cell target epitopes selected from SEQ ID NO: 2-57 (S2-10, S1220-1228, S1000-1008, S958-966, E20-28, ORF1ab1675-1683, ORF1ab2363-2371, ORF1ab3013-3021, ORF1ab3183-3191, ORF1ab5470-5478, ORF1ab6749-6757, ORF7b26-34, ORF8a73-81, ORF103-11, and ORF105-13). In some embodiments, the one or more conserved coronavirus CD4+ T cell target epitopes selected from SEQ ID NO: 58-105 (ORF1a1350-1365, ORF1ab5019-5033, ORF612-26, ORF1ab6088-6102. ORF1ab6420-6434, ORF1a1801-1815, S1-13, E26-40, E20-34, M176-190, N388-403, ORF7a3-17, ORF7a1-15, ORF7b8-22, ORF7a98-112, and ORF81-15.). In some embodiments, the one or more conserved coronavirus CD8+ T cell target epitopes selected from SEQ ID NO: 106-138 (S287-317, S524-598, S601-640, S802-819, 3888-909, S369-393, S440-501, S1133-1172, S329-363, and S13-37).

In some embodiments, the partial spike protein comprises a trimerized SARS-CoV-2 receptor-binding domain (RBD). In some embodiments, the whole spike protein or partial spike protein has an intact S1-S2 cleavage site. In some embodiments, the spike protein is stabilized with proline substitutions at amino acid positions 986 and 987.

The present invention also features a pan-coronavirus recombinant vaccine composition comprising one of SEQ ID NO: 139-147.

The present invention also includes the corresponding nucleic acid sequences for any of the protein sequences herein. The present invention also includes the corresponding protein sequences for any of the nucleic acid sequences herein.

Embodiments herein may comprise whole spike protein or a portion of spike protein. Whole spike protein and a portion thereof is not limited to a wild type or original sequence and may include spike protein or a portion thereof with one or more modifications and/or mutations, such as point mutations, deletions, etc.; including the mutations described herein such as those for improving stability.

Embodiments of the present invention can be freely combined with each other if they are not mutually exclusive.

Any feature or combination of features described herein are included within the scope of the present invention provided that the features included in any such combination are not mutually inconsistent as will be apparent from the context, this specification, and the knowledge of one of ordinary skill in the art.

Additional advantages and aspects of the present invention are apparent in the following detailed description and claims.

DESCRIPTION OF THE SEVERAL VIEWS OF THE DRAWING(S)

The features and advantages of the present invention will become apparent from a consideration of the following detailed description presented in connection with the accompanying drawings in which:

FIG. 1 shows a schematic view of an example of a large sequence pan-coronavirus recombinant vaccine composition. Each large sequence in the recombinant vaccine composition may comprise epitopes. CD8+ T cell epitopes are shown with a square, CD4+ T cell epitopes are shown with a circle and B-cell epitopes are shown with a diamond. Each shape (square, circle, or diamond) may represent a variety of different epitopes and is not limited to a singular epitope. The multi-epitope pan-coronavirus vaccines are not limited to a specific combination of large sequences as shown. The large sequence pan-coronavirus vaccines may comprise a various number of large sequences.

FIG. 2A shows an evolutionary comparison of genome sequences among beta-Coronavirus strains isolated from humans and animals. A phylogenetic analysis performed between SARS-CoV-2 strain sp (obtained from humans (Homo Sapiens (black)), along with the animal's SARS-like Coronaviruses genome sequence (SL-CoVs) sequences obtained from bats (Rhinolophus affinis, Rhinolophus malayanus (red)), pangolins (Manis javanica (blue)), civet cats (Paguma larvata (green)), and camels (Camelus dromedaries (Brown)). The included SARS-CoV/MERS-CoV strains are from previous outbreaks (obtained from humans (Urbani, MERS-CoV, OC43, NL63, 229E, HKU1-genotype-B), bats (WIV16, WIV1, YNLF-31C, Rs672, recombinant strains), camel (Camelus dromedaries, (KT368891.1, MN514967.1, KF917527.1, NC_028752.1), and civet (Civet007, A022, B039)). The human SARS-CoV-2 genome sequences are represented from six continents.

FIG. 28 shows an evolutionary analysis performed among the human-SARS-CoV-2 genome sequences reported from six continents and SARS-CoV-2 genome sequences obtained from bats (Rhinolophus affinis, Rhinolophus malayanus), and pangolins (Manis javanica)).

FIG. 3A shows lungs, heart, kidneys, intestines, brain, and testicles express ACE2 receptors and are targeted by SARS-CoV-2 virus. SARS-CoV-2 virus docks on the Angiotensin converting enzyme 2 (ACE2) receptor via spike surface protein.

FIG. 3B shows a System Biology Analysis approach utilized in the present invention.

FIG. 4 shows sequence homology analysis for SARS-CoV-2, common cold CoV strains, MERS, SARS-CoV-Urbani and animal CoVs with SARS-CoV-2 Wuhan Strain (Query strain; hCoV-19batYN01). Five fragments SARS-CoV-2 genome were found to be highly conserved (1 bp-1580 bp (fragment 1), 3547 bp-12830 bp (fragment 2), 17472 bp-21156 bp (fragment 3), 22584 bp-24682 bp (fragment 4), and 26193 bp-27421 bp (fragment 5).

FIG. 5 shows sequence homology analysis for fragment 1 (1 bp-1580 bp) which comprises portions of ORF1a/b. The Query sequence (1-1580 bp hCoV-19/batYN1) was BLAST against all the SARS-CoV-2 VOCs, human CoV strains, CoV strains from bats, pangolin, civet cat. 28 variants/strains were found with significant homology for this queried region.

FIG. 6 shows sequence homology analysis for fragment 2 (3547 bp-12830 bp). The Query sequence (3547-12830 bp hCoV-19/batYN01) was BLAST against all the SARS-CoV-2 VOCs, human CoV strains; CoV strains from bats, pangolin, civet cats. 30 variants/strains were found with significant homology for this queried region.

FIG. 7 shows sequence homology analysis for fragment 3 (17472 bp-21156 bp). The Query sequence (17472-21156 bp hCoV-19/batYN01) was BLAST against all the SARS-CoV-2 VOCs, human CoV strains, CoV strains from bats, pangolin, civet cats. 29 variants/strains were found with significant homology for this queried region.

FIG. 8 shows sequence homology analysis for fragment 4 (22584 bp-24682 bp) which comprises the spike protein. The Query sequence (22584-24682 bp hCoV-19/batYN01) was BLAST against all the SARS-CoV-2 VOCs, human CoV strains, CoV strains from bats, pangolin, civet cats. 29 variants/strains were found with significant homology for this queried region.

FIG. 9 shows sequence homology analysis for fragment 5 (26193 bp-27421 bp). The Query sequence (26193-27421 bp hCoV-19/batYN01) was BLAST against all the SARS-CoV-2 VOCs, human CoV strains, CoV strains from bats, pangolin, civet cats. 31 variants/strains were found with significant homology for this queried region.

FIG. 10 shows a sequence homology analysis to screen conservancy of potential SARS-CoV-2-derived human CD8+ T cell epitopes. Shown are the comparison of sequence homology for the potential CD8+ T cell epitopes among 81,963 SARS-CoV-2 strains (that currently circulate in 190 countries on 6 continents), the 4 major “common cold” Coronaviruses that cased previous outbreaks (i.e. hCoV-OC43, hCoV-229E, hCoV-HKU1-Genotype B, and hCoV-NL63), and the SL-CoVs that were isolated from bats, civet cats, pangolins and camels. Epitope sequences highlighted in yellow present a high degree of homology among the currently circulating 81,963 SARS-CoV-2 strains and at least a 50% conservancy among two or more humans SARS-CoV strains from previous outbreaks, and the SL-CoV strains isolated from bats, civet cats, pangolins and camels, as described herein. Homo Sapiens—black, bats (Rhinolophus affinis, Rhinolophus malayanus—red), pangolins (Manis javanica—blue), civet cats (Paguma larvata—green), and camels (Camelus dromedaries-brown).

FIG. 11A shows docking of highly conserved SARS-CoV-2-derived human CD8+ T cell epitopes to HLA-A*02:01 molecules, e.g., docking of the 27 high-affinity CD8+ T cell binder peptides to the groove of HLA-A*02:01 molecules.

FIG. 11B shows a summary of the interaction similarity scores of the 27 high-affinity CDB8+ T cell epitope peptides to HLA-A*02:01 molecules determined by protein-peptide molecular docking analysis. Black columns depict CD8+ T cell epitope peptides with high interaction similarity scores.

FIG. 12A shows an experimental design show CD8+ T cells are specific to highly conserved SARS-CoV-2 epitopes detected in COVID-19 patients and unexposed healthy individuals: PBMCs from HLA-A*02:01 positive COVID-19 patients (n=30) and controls unexposed healthy individuals (n=10) were isolated and stimulated overnight with 10 μM of each of the 27 SARS-CoV-2-derived CD8+ T cell epitopes. The number of IFN-γ-producing cells were quantified using ELISpot assay.

FIG. 12B shows the results from FIG. 12A. Dotted lines represent threshold to evaluate the relative magnitude of the response: a mean SFCs between 25 and 50 correspond to a medium/intermediate response whereas a strong response is defined for a mean SFCs >50.

FIG. 12C shows the results from experiments where PBMCs from HLA-A*02:01 positive COVID-19 patients were further stimulated for an additional 5 hours in the presence of mAbs specific to CD107a and CD107b, and Golgi-plug and Golgi-stop. Tetramers specific to Spike epitopes, CD107a/b and CD69 and TNF-expression were then measured by FACS. Representative FACS plot showing the frequencies of Tetramer+CD8+ T cells, CD107a % b+CD8+ T cells, CD69+CD8+ T cells and TNF-+CDB8+ T cells following priming with a group of 4 Spike CD8+ T cell epitope peptides. Average frequencies of tetramer+CD8+ T cells, CD107a/b+CD8+ T cells, CD69+CD8+ T cells and TNF-+CD8+ T cells.

FIG. 13A shows a timeline of immunization and immunological analyses for experiments testing the immunogenicity of genome-wide identified human SARS-CoV-2 CD8+ T epitopes in HLA-A*02:01/HLA-DRB1 double transgenic mice. Eight groups of age-matched HLA-A*02:01 transgenic mice (n=3) were immunized subcutaneously, on days 0 and 14, with a mixture of four SARS-CoV-2-derived human CD8+ T cell peptide epitopes mixed with PADRE CD4+ T helper epitope, delivered in alum and CpG1826 adjuvants. As a negative control, mice received adjuvants alone (mock-immunized).

FIG. 13B shows the gating strategy used to characterize spleen-derived CD8+ T cells. Lymphocytes were identified by a low forward scatter (FSC) and low side scatter (SSC) gate. Singlets were selected by plotting forward scatter area (FSC-A) vs. forward scatter height (FSC-H). CD8 positive cells were then gated by the expression of CD8 and CD3 markers.

FIG. 13C shows a representative ELISpot image (left panel) and average frequencies (right panel) of IFN-γ-producing cell spots from splenocytes (106 cells/well) stimulated for 48 hours with 10 μM of 10 immunodominant CD8+ T cell peptides and 1 subdominant CD8+ T cell peptide out of the total pool of 27 CD8+ T cell peptides derived from SARS-CoV-2 structural and non-structural proteins. The number on the top of each ELISpot image represents the number of IFN-γ-producing spot forming T cells (SFC) per one million splenocytes.

FIG. 13D shows a representative FACS plot (left panel) and average frequencies (right panel) of IFN-γ and TNF-production by, and CD107a/b and CD69 expression on 10 immunodominant CD8+ T cell peptides and 1 subdominant CD8+ T cell peptide out of the total pool of 27 CD8+ T cell peptides derived from SARS-CoV-2 structural and non-structural proteins determined by FACS. Numbers indicate frequencies of IFN-γ+CD8+ T cells, CD107+CD8+ T cells, CD69+CD8+ T cells and TNF-+CD8+ T cells, detected in 3 immunized mice.

FIG. 14 shows the SARS-CoV/SARS-CoV-2 genome encodes two large non-structural genes ORF1a (green) and ORF1b (gray), encoding 16 non-structural proteins (NSP1-NSP16). The genome encodes at least six accessory proteins (shades of light grey) that are unique to SARS-CoV/SARS-CoV-2 in terms of number, genomic organization, sequence, and function. The common SARS-CoV, SARS-CoV-2 and SL-CoVs-derived human B (blue); CD4+ (green) and CD8+ (black) T cell epitopes are shown. Structural and non-structural open reading frames utilized in this study were from SARS-CoV-2-Wuhan-Hu-1 strain (NCBI accession number MN908947.3, SEQ ID NO: 1). The amino acid sequence of the SARS-CoV-2-Wuhan-Hu-1 structural and non-structural proteins was screened for human B, CD4+ and CD8+ T cell epitopes using different computational algorithms as described herein. Shown are genome-wide identified SARS-CoV-2 human B cell epitopes (in blue), CD4+ T cell epitopes (in green), CD8+ T cell epitopes (in black) that are highly conserved between human and animal Coronaviruses.

FIG. 15 shows the identification of highly conserved potential SARS-CoV-2-derived human CD4+ T cell epitopes that bind with high affinity to HLA-DR molecules: Out of a total of 9,594 potential HLA-DR-restricted CD4+ T cell epitopes from the whole genome sequence of SARS-CoV-2-Wuhan-Hu-1 strain (MN908947.3), 16 epitopes that bind with high affinity to HLA-DRB1 molecules were selected. The conservancy of the 16 CD4+ T cell epitopes was analyzed among human and animal Coronaviruses. Shown are the comparison of sequence homology for the 16 CD4+ T cell epitopes among 81,963 SARS-CoV-2 strains (that currently circulate in 6 continents), the 4 major “common cold” Coronaviruses that cased previous outbreaks (i.e. hCoV-OC43, hCoV-229E, hCoV-HKU1, and hCoV-NL63), and the SL-CoVs that were isolated from bats, civet cats, pangolins and camels. Epitope sequences highlighted in green present high degree of homology among the currently circulating 81,963 SARS-CoV-2 strains and at least a 50% conservancy among two or more humans SARS-CoV strains from previous outbreaks, and the SL-CoV strains isolated from bats, civet cats, pangolins and camels, as described in Materials and Methods. Homo Sapiens—black, bats (Rhinolophus affinis, Rhinolophus malayanus—red), pangolins (Manis javanica—blue), civet cats (Paguma larvata—green), and camels (Camelus dromedaries—brown).

FIG. 16A the molecular docking of highly conserved SARS-CoV-2 CD4+ T cell epitopes to HLA-DRBI molecules. Molecular docking of 16 CD4+ T cell epitopes, conserved among human SARS-CoV-2 strains, previous humans SARS/MERS-CoV and bat SL-CoVs into the groove of the HLA-DRB1 protein crystal structure (PDB accession no: 4UQ3) was determined using the GalaxyPepDock server. The 16 CD4+ T cell epitopes are promiscuous restricted to HLA-DRB1*01:01, HLA-DRB1*11:01, HLA-DRB1*15:01, HLA-DRB1*03:01 and HLA-DRB1*04:01 alleles. The CD4+ T cell peptides are shown in ball and stick structures, and the HLA-DRB1 protein crystal structure is shown as a template. The prediction accuracy is estimated from a linear model as the relationship between the fraction of correctly predicted binding site residues and the template-target similarity measured by the protein structure similarity score (TM score) and interaction similarity score (Sinter) obtained by linear regression. Sinter shows the similarity of the amino acids of the CD8+ T cell peptides aligned to the contacting residues in the amino acids of the HLA-DRB1 template structure.

FIG. 16B shows histograms representing interaction similarity score of CD4+ T cells specific epitopes observed from the protein-peptide molecular docking analysis.

FIG. 17A shows an experimental design to show CD4+ T cells are specific to highly conserved SARS-CoV-2 epitopes detected in COVID-19 patients and unexposed healthy individuals: PBMCs from HLA-DRB1 positive COVID-19 patients (n=30) and controls unexposed healthy individuals (n=10) were isolated and stimulated for 48 hrs. with 10 μM of each of the 16 SARS-CoV-2-derived CD4+ T cell epitopes. The number of IFN-producing cells were quantified using ELISpot assay.

FIG. 17B shows the results from FIG. 17A. Dotted lines represent a threshold to evaluate the relative magnitude of the response: a mean SFCs between 25 and 50 correspond to a medium/intermediate response, whereas a strong response is defined for a mean SFCs >50. PBMCs from HLA-DRB1-positive COVID-19 patients

FIG. 17C shows the results from further stimulating for an additional 5 hours in the presence of mAbs specific to CD107a and CD107b, and Golgi-plug and Golgi-stop. Tetramers specific to two Spike epitopes, CD107a/b and CD69 and TNF-alpha expression were then measured by FACS. Representative FACS plot showing the frequencies of Tetramer+CD4+ T cells, CD107a/b+CD4+ T cells, CD69+CD4+ T cells and TNF-+CD4+ T cells following priming with a group of 2 Spike CD4+ T cell epitope peptides. Average frequencies are shown for tetramer+CD4+ T cells, CD107a/b+CD4+ T cells, CD69+CD4+ T cells and TNF-+CD4+ T cells.

FIG. 18A shows a timeline of immunization and immunological analyses for testing immunogenicity of genome-wide identified human SARS-CoV-2 CD4.+ T epitopes in HLA-A*02:01/HLA-DRB1 double transgenic mice. Four groups of age-matched HLA-DRB1 transgenic mice (n=3) were immunized subcutaneously, on days 0 and 14, with a mixture of four SARS-CoV-2-derived human CD4+ T cell peptide epitopes delivered in alum and CpG1826 adjuvants. As a negative control, mice received adjuvants alone (mock-immunized).

FIG. 18B shows the gating strategy used to characterize spleen-derived CD4+ T cells. CD4 positive cells were gated by the CD4 and CD3 expression markers.

FIG. 18C shows the representative ELISpot images (left panel) and average frequencies (right panel) of IFN-γ-producing cell spots from splenocytes (106 cells/well) stimulated for 48 hours with 10 μM of 7 immunodominant CD4+ T cell peptides and 1 subdominant CD4+ T cell peptide out of the total pool of 16 CD4+ T cell peptides derived from SARS-CoV-2 structural and non-structural proteins. The number of IFN-γ-producing spot forming T cells (SFC) per one million of total cells is presented on the top of each ELISpot image.

FIG. 18D shows the representative FACS plot (left panel) and average frequencies (right panel) show IFN-γ and TNF-α-production by, and CD107a/b and CD69 expression on 7 immunodominant CD4+ T cell peptides and 1 subdominant CD4+ T cell peptide out of the total pool of 16 CD4+ T cell peptides derived from SARS-CoV-2 determined by FACS. The numbers indicate percentages of IFN-γ+CD4+ T cells, CD107+CD4+ T cells, CD69+CD4+ T cells and TNF-α+CD4+ T cells detected in 3 immunized mice.

FIG. 19 shows the conservation of Spike-derived B cell epitopes among human, bat, civet cat, pangolin, and camel coronavirus strains: Multiple sequence alignment performed using ClustalW among 29 strains of SARS coronavirus (SARS-CoV) obtained from human, bat, civet, pangolin, and camel. This includes 7 human SARS/MERS-CoV strains (SARS-CoV-2-Wuhan (MN908947.3), SARS-HCoV-Urbani (AY278741.1), CoV-HKU1-Genotype-B (AY884001), CoV-OC43 (KF923903), CoV-NL63 (NC005831), CoV-229E (KY983587), MERS (NC019843)); 8 bat SARS-CoV strains (BAT-SL-CoV-WIV16 (KT444582), BAT-SL-CoV-WIV1 (KF367457.1), BAT-SL-CoV-YNLF31C (KP886808.1), BAT-SARS-CoV-RS672 (FJ588686.1), BAT-CoV-RATG13 (MN996532.1), BAT-CoV-YN01 (EPIISL412976), BAT-CoV-YNO2 (EPIISL412977), BAT-CoV-19-ZXC21 (MG772934.1); 3 Civet SARS-CoV strains (SARS-CoV-Civet007 (AY572034.1), SARS-CoV-A022 (AY686863.1), SARS-CoV-B039 (AY686864.1)); 9 pangolin SARS-CoV strains (PCoV-GX-P2V (MT072864.1), PCoV-GX-P5E (MT040336.1), PCoV-GX-P5L (MT040335.1), PCoV-GX-P1E (MT040334.1), PCoV-GX-P4L (MT040333.1), PCoV-MP789 (MT084071.1), PCoV-GX-P3B (MT072865.1), PCoV-Guangdong-P2S (EPIISL410544), PCoV-Guangdong (EPHSL410721)); 4 camel SARS-CoV strains (Camel-CoV-HKU23 (KT368891.1), DcCoV-HKU23 (MN514967.1), MERS-CoV-Jeddah (KF917527.1), Riyadh/RY141 (NC028752.1)) and 1 recombinant strain (FJ211859.1)). Regions highlighted with blue color represent the sequence homology. The B cell epitopes, which showed at least 50% conservancy among two or more strains of the SARS Coronavirus or possess receptor-binding domain (RBD) specific amino acids were selected as candidate epitopes.

FIG. 20A shows the docking of SARS-CoV-2 Spike glycoprotein-derived B cell epitopes to human ACE2 receptor, e.g., molecular docking of 22 B-cell epitopes, identified from the SARS-CoV-2 Spike glycoprotein, with ACE2 receptors. B cell epitope peptides are shown in ball and stick structures whereas the ACE2 receptor protein is shown as a template. S471-501 and S369-393 peptide epitopes possess receptor binding domain region specific amino acid residues. The prediction accuracy is estimated from a linear model as the relationship between the fraction of correctly predicted binding site residues and the template-target similarity measured by the protein structure similarity score and interaction similarity score (Sinter) obtained by linear regression. Sinter shows the similarity of amino acids of the B-cell peptides aligned to the contacting residues in the amino acids of the ACE2 template structure. Higher Sinter score represents a more significant binding affinity among the ACE2 molecule and B-cell peptides.

FIG. 20B shows the summary of the interaction similarity score of 22 B cells specific epitopes observed from the protein-peptide molecular docking analysis. B cell epitopes with high interaction similarity scores are indicated in black.

FIG. 21A shows the timeline of immunization and immunological analyses for testing to show IgG antibodies are specific to SARS-CoV-2 Spike protein-derived B-cell epitopes in immunized B6 mice and in convalescent COVID-19 patients. A total of 22 SARS-CoV-2 derived B-cell epitope peptides selected from SARS-CoV-2 Spike protein and tested in B6 mice were able to induce antibody responses. Four groups of age-matched B6 mice (n=3) were immunized subcutaneously, on days 0 and 14, with a mixture of 4 or 5 SARS-CoV-2 derived B-cell peptide epitopes emulsified in alum and CpG1826 adjuvants. AlumCpG1826 adjuvants alone were used as negative controls (mock-immunized).

FIG. 21B shows the frequencies of IgG-producing CD3(−)CD138(+)B220(+) plasma B cells were determined in the spleen of immunized mice by flow cytometry. For example, FIG. 21B shows the gating strategy was as follows: Lymphocytes were identified by a low forward scatter (FSC) and low side scatter (SSC) gate. Singlets were selected by plotting forward scatter area (FSC-A) versus forward scatter height (FSC-H). B cells were then gated by the expression of CD3(−) and B220(+) cells and CD138 expression on plasma B cells determined.

FIG. 21C shows the frequencies of IgG-producing CD3(−)CD138(+)B220(+) plasma B cells were determined in the spleen of immunized mice by flow cytometry. For example, FG 15C shows a representative FACS plot (left panels) and average frequencies (right panel) of plasma B cells detected in spleen of immunized mice. The percentages of plasma CD138(−)B220(+)B cells are indicated on the top left of each dot plot,

FIG. 21D shows SARS-CoV-2 derived B-cell epitopes-specific IgG responses were quantified in immune serum, 14 days post-second immunization (i.e. day 28), by ELISpot (Number of IgG(+)Spots). Representative ELISpot images (left panels) and average frequencies (right panel) of anti-peptide specific IgG-producing B cell spots (1×106 splenocytes/well) following 4 days in vitro B cell polyclonal stimulation with mouse Poly-S (Immunospot). The top/left of each ELISpot image shows the number of IgG-producing B cells per half a million cells. ELISA plates were coated with each individual immunizing peptide.

FIG. 21E shows the B-cell epitopes-specific IgG concentrations (μg/mL) measured by ELISA in levels of IgG detected in peptide-immunized 86 mice, after subtraction of the background measured from mock-vaccinated mice. The dashed horizontal line indicates the limit of detection.

FIG. 21F and FIG. 21G show the B-cell epitopes-specific IgG concentrations (μg/mL) measured by ELISA in Level of IgG specific to each of the 22 Spike peptides detected SARS-CoV-2 infected patients (n=40), after subtraction of the background measured from healthy non-exposed individuals (n=10). Black bars and gray bars show high and medium immunogenic B cell peptides, respectively. The dashed horizontal line indicates the limit of detection.

FIG. 22 shows an example of a whole spike protein comprising mutations including 6 proline mutations. The 6 proline mutations comprise single point mutations F817P, A892P, A899P, A942P, K986P and V987P. Additionally, the spike protein comprises a 682-QQAQ-685 mutation of the furin cleavage site for protease resistance. In some embodiments, the K986P and V987P Mutations allow for perfusion stabilization. FIG. 22 also shows the following sequences: MFVFLVLLPLVSS (SEQ ID NO: 63), ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGC (SEQ ID NO: 422), CAGCAGGCCCAG (SEQ ID NO: 423), and CCCCCC.

FIG. 23 shows non-limiting examples of how the large sequences of the compositions described herein may be arranged.

FIG. 24 shows a schematic representation of a prototype Coronavirus vaccine of the present invention. The present invention is not limited to the prototype coronavirus vaccines as shown.

FIG. 25A shows a non-limiting example of a method for delivering the vaccine composition described herein using a “prime/pull” regimen in humans. The method comprises administering a pan-coronavirus recombinant vaccine composition and further administering at least one T-cell attracting chemokine (e.g. CXCL11) after administering the pan-coronavirus recombinant vaccine composition.

FIG. 25B shows a non-limiting example of a method for delivering the vaccine composition described herein using a “prime/boost” regimen in humans. The method comprises administering a first composition, e.g., a first pan-coronavirus recombinant vaccine composition dose using a first delivery system and further administering a second composition, e.g., a second vaccine composition dose using a second delivery system. In some embodiments, the first delivery system and the second delivery system are different.

FIG. 25C shows a non-limiting example of a method for delivering the vaccine composition described herein using a “prime/pull/keep” regimen in humans to increase the size and maintenance of lung-resident B-cells, CD4+ T cells and CD8+ T cells to protect against SARS-CoV-2. The method comprises administering a pan-coronavirus recombinant vaccine composition and administering at least one T-cell attracting chemokine (e.g. CXCL11 or CXCL17) after administering the pan-coronavirus recombinant vaccine composition.

FIG. 25D shows a non-limiting example of a method for delivering the vaccine composition described herein using a “prime/pull/boost” regimen in humans to increase the size and maintenance of lung-resident B-cells, CD4+ T cells and CD8+ T cells to protect against SARS-CoV-2. The method comprises administering a pan-coronavirus recombinant vaccine composition and administering at least one T-cell attracting chemokine (e.g. CXCL11 or CXCL17) after administering the pan-coronavirus recombinant vaccine composition. The method further comprises administering at least one cytokine after administering the T-cell attracting chemokine (e.g. IL-7, IL-5, or IL-2).

FIG. 26A shows a non-limiting example of a method for delivering the vaccine composition described herein using a “prime/pull” regimen in domestic animals (e.g. cats or dogs). The method comprises administering a pan-coronavirus recombinant vaccine composition and further administering at least one T-cell attracting chemokine (e.g. CXCL11) after administering the pan-coronavirus recombinant vaccine composition.

FIG. 26B shows a non-limiting example of a method for delivering the vaccine composition described herein using a “prime/boost” regimen in domestic animals (e.g. cats or dogs). The method comprises administering a first composition, e.g., a first pan-coronavirus recombinant vaccine composition dose using a first delivery system and further administering a second composition, e.g., a second vaccine composition dose using a second delivery system. In some embodiments, the first delivery system and the second delivery system are different.

FIG. 26C shows a non-limiting example of a method for delivering the vaccine composition described herein using a “prime/pull/keep” regimen in domestic animals (e.g. cats or dogs) to increase the size and maintenance of lung-resident B-cells, CD4+ T cells and CD8+ T cells to protect against SARS-CoV-2. The method comprises administering a pan-coronavirus recombinant vaccine composition and administering at least one T-cell attracting chemokine (e.g. CXCL11 or CXCL17) after administering the pan-coronavirus recombinant vaccine composition.

FIG. 26D shows a non-limiting example of a method for delivering the vaccine composition described herein using a “prime/pull/boost” regimen in domestic animals (e.g. cats or dogs) to increase the size and maintenance of lung-resident B-cells, CD4+ T cells and CD8+ T cells to protect against SARS-CoV-2. The method comprises administering a pan-coronavirus recombinant vaccine composition and administering at least one T-cell attracting chemokine (e.g. CXCL11 or CXCL17) after administering the pan-coronavirus recombinant vaccine composition. The method further comprises administering at least one cytokine after administering the T-cell attracting chemokine (e g. IL-7, IL-5, or IL-2),

FIGS. 27A, 27B, and 27C shows experimental evidence showing immunization with pool of CD8⁺ T cell, CD4⁺ T cell, and B cell peptides to provide protection against all the current SARS-CoV-2 variants of concerns in triple transgenic h-ACE2-HLA-A2/DR mice. Viral titration (FIG. 27A), weight loss (FIG. 27B), and survival (FIG. 27C) data have been shown.

FIG. 28 shows non-limiting configurations of recombinant vaccine compositions described herein.

FIGS. 29A and 29B show non-limiting examples of recombinant hybrid vaccine compositions described herein. The proteins may be covalently or non-covalently linked together for administration of the vaccine composition. Note: “Nsp” may refer to Nsp1, Nsp2, Nsp3, Nsp5, Nsp6, Nsp7, Nsp8, Nsp9, Nsp10, Nsp12, Nsp13, Nsp14, Nsp15, Nsp16, or a combination thereof, “Spike protein” may refer to a portion of the spike protein, or a spike protein with one or more mutations (e.g., a spike protein with two or six proline substitutions).

FIG. 30 shows the results of a sequence alignment of various influenza viruses and variants and the resulting conserved region.

FIG. 31 shows non-limiting examples of recombinant vaccine compositions described herein.

FIG. 32A shows non-limiting examples of influenza proteins and SARS-CoV2 proteins that may be used in the vaccine compositions as described herein; these proteins may be used in any combination to create said compositions. Note: Nsp may refer to Nsp1, Nsp2, Nsp3, Nsp5, Nsp6, Nsp7, Nsp8, Nsp9, Nsp10, Nsp12, Nsp13. Nsp14. Nsp15, Nsp16, or a combination thereof.

FIG. 32B shows non-limiting examples of recombinant hybrid vaccine compositions described herein. The proteins may be covalently or non-covalently linked together for administration of the vaccine composition. Note: “Nsp” may refer to Nsp1, Nsp2, Nsp3, Nsp5, Nsp6, Nsp7, Nsp8, Nsp9, Nsp10, Nsp12, Nsp13, Nsp14, Nsp15, Nsp16, or a combination thereof, “Spike protein” may refer to a portion of the spike protein, or a spike protein with one or more mutations (e.g., a spike protein with two or six proline substitutions).

FIG. 33 shows schematic views of non-limiting examples of vaccine compositions showing an optional molecular adjuvant, T cell attracting chemokine, and/or composition for promoting T cell proliferation, as well as non-limiting examples of orientations of said optional molecular adjuvant, T cell attracting chemokine, and/or composition for promoting T cell proliferation.

FIG. 34A shows an experimental plan to determine the efficacy of (1) Ad5-Pan-CoV-Vaccines and (2) mRNA-LNP vaccine against the SARS-CoV-2 Delta (B.1.617.2) variant in golden hamsters. Briefly, at Day 0 the hamsters were immunized with mRNA-LNP vaccines (off the shelf LNP; volume: 50 μl); at Day 14 the hamsters were immunized intramuscularly with Ad5 vaccines (as per doses mentioned FIG. 34B: Volume: 50 μl (Ad5 vector+PBS)); at Day 21 the hamsters were immunized with mRNA-LNP vaccines (off the shelf LNP: Volume: 50 μl). For mRNA-LNP immunized hamsters: Infection 42 days after first immunization with 5×10⁵pfu of SARS-CoV-2 B.617.2 variant (Delta) intranasally (total in both nostril) Total volume of virus given: 208 μl by the intranasal route. For Ad5 immunized hamsters: Infection 28 days after first immunization with 5×10⁵pfu of SARS-CoV-2 B.617.2 variant (Delta) intranasally (total in both nostril) Total volume of virus given: 208 μl by the intranasal route.

FIG. 34B shows the doses given of each vaccine.

FIG. 35 de-risking of program demonstration of additive protective effect of T cells and antibodies. qRT-PCR data shows a reduction of viral copy number for hamsters immunized with Ad-5 Pan-CoV-Vaccines (i) Spike-6P (10⁸)+Nucleocapsid (10¹⁰) and (fi) Spike-6P (10⁸)+NSP3 (10¹⁰) against the SARS-CoV-2 Delta (B.1.6117.2) variant in golden hamsters.

FIGS. 36A and 36B show de-risking of program demonstration of additive protective effect of T cells and antibodies. FIG. 36A shows Physical estimation for hamsters immunized with Ad-5 Pan-CoV-Vaccines (i) Spike-6P (10⁸), (ii) Spike-61P (10⁸)+NSP3 (10¹⁰), (iii) NSP3 (10¹⁰) against the SARS-CoV-2 Delta (B.1.617.2) variant. FIG. 36B shows physical estimation for hamsters immunized with Ad-5 Pan-CoV-Vaccines (I) Spike-6P (10⁸), (ii) Spike-6P (10⁸)+Nucleocapsid (10¹⁰), (iii) Nucleocapsid (10¹⁰) against the SARS-CoV-2 Delta (B.1.617.2) variant. The arrows indicate an early additive or synergetic beneficial protective effect of adding Nucleoprotein to Spike.

FIGS. 37A and 378 show hamsters challenged with SARS-CoV-2 Delta variant then rechallenged with Omicron variant.

FIG. 38 shows qRT-PCR data demonstrating reduced viral copy number for hamsters immunized with Ad-5 Pan-CoV-Vaccines (i) Spike-6P (10⁸)+Nucleocapsid (10¹⁰) and (ii) Spike-6P (10⁸)+NSP3 (10¹⁰) against the SARS-CoV-2 Omicron variant in golden hamsters. The hamsters were immunized with Ad5 vectors and challenged initially with Delta variant on Day 28 post immunization. Subsequently the same group of animals were re-challenged with Omicron variant to see the beneficial additive or synergetic protective effect of adding Nucleoprotein to Spike.

FIG. 39 shows de-risking of program demonstration of additive protective effect of T cells and antibodies. Hamsters challenged with SARS-CoV-2 Delta variant.

FIG. 40 shows neutralizing antibodies produced against SARS-CoV-2 Delta variant.

FIG. 41 shows preclinical PoC of prototype T plus B cell SARS-CoV-2 vaccines Nucleoprotein plus Spike. Hamsters challenged with SARS-CoV-2 Washington variant.

FIG. 42 shows anti-SARS-CoV-2 Spike specific IgG measured in the serum of Hamsters immunized with, LNP encapsulated mRNA containing Spike-Hexapro alone and Spike-Hexapro mixed with Nucleoprotein on day 10, 40 and 70 after second immunization. Time kinetics based on ELISA data showing statistically significant increase in spike specific antibody in the serum of hamsters immunized with Spike-Hexapro mixed with Nucleoprotein compared to Spike-Hexapro alone. Plates were coated with 100 ng of Spike (S1+S2) from Sino biological.

FIG. 43 shows the protective role of T cells against virus. ACE-2/HLA-I/HLA-II transgenic mice were challenge 10⁴pfu of SARS-CoV-2 Washington variant.

FIG. 44 shows non-limiting examples of how the vaccine compositions described herein may be administered.

TERMS

Unless otherwise explained, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which a disclosed invention belongs. The singular terms “a,” “an,” and “the” include plural referents unless context clearly indicates otherwise. Similarly, the word “or” is intended to include “and” unless the context clearly indicates otherwise. The term “comprising” means that other elements can also be present in addition to the defined elements presented. The use of “comprising” indicates inclusion rather than limitation. Stated another way, the term “comprising” means “including principally, but not necessary solely”. Furthermore, variation of the word “comprising”, such as “comprise” and “comprises”, have correspondingly the same meanings. In one respect, the technology described herein related to the herein described compositions, methods, and respective component(s) thereof, as essential to the invention, yet open to the inclusion of unspecified elements, essential or not (“comprising”).

Suitable methods and materials for the practice and/or testing of embodiments of the disclosure are described below. Such methods and materials are illustrative only and are not intended to be limiting. Other methods and materials similar or equivalent to those described herein can be used. For example, conventional methods well known in the art to which the disclosure pertains are described in various general and more specific references, including, for example, Sambrook et. al., Molecular Cloning: A Laboratory Manual, 2d ed., Cold Spring Harbor Laboratory Press, 1989; Sambrook et al., Molecular Cloning: A Laboratory Manual, 3d ed., Cold Spring Harbor Press, 2001; Ausubel et al., Current Protocols in Molecular Biology, Greene Publishing Associates, 1992 (and Supplements to 2000); Ausubel et al., Short Protocols in Molecular Biology: A Compendium of Methods from Current Protocols in Molecular Biology, 4th ed., Wiley & Sons, 1999; Harlow and Lane, Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory Press, 1990; and Harlow and Lane, Using Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory Press, 1999, Gene Expression Technology (Methods in Enzymology, Vol. 185, edited by D. Goeddel, 1991. Academic Press, San Diego, Calif.), “Guide to Protein Purification” in Methods in Enzymology (M. P. Deutshcer, ed., (1990) Academic Press, Inc.); PCR Protocols: A Guide to Methods and Applications (Innis, et al. 1990. Academic Press, San Diego, Calif.), Culture of Animal Cells: A Manual of Basic Technique, 2^ndEd. (R. I. Freshney. 1987. Liss, Inc. New York, N.Y.), Gene Transfer and Expression Protocols, pp. 109-128, ed. E. J. Murray, The Humana Press Inc., Clifton, N.J.), and the Ambion 1998 Catalog (Ambion, Austin, Tex.), the disclosures of which are incorporated in their entirety herein by reference.

Although methods and materials similar or equivalent to those described herein can be used to practice or test the disclosed technology, suitable methods and materials are described below. The materials, methods, and examples are illustrative only and not intended to be limiting.

As used herein, the terms “immunogenic protein, polypeptide, or peptide” or “antigen” refer to polypeptides or other molecules (or combinations of polypeptides and other molecules) that are immunologically active in the sense that once administered to the host, it is able to evoke an immune response of the humoral and/or cellular type directed against the protein. In embodiments, the protein fragment has substantially the same immunological activity as the total protein. Thus, a protein fragment according to the disclosure can comprise or consist essentially of or consist of at least one epitope or antigenic determinant. An “immunogenic” protein or polypeptide, as used herein, may include the full-length sequence of the protein, analogs thereof, or immunogenic fragments thereof. “Immunogenic fragment” refers to a fragment of a protein which includes one or more epitopes and thus elicits the immunological response described above.

Synthetic antigens are also included within the definition, for example, poly-epitopes, flanking epitopes, and other recombinant or synthetically derived antigens. Immunogenic fragments for purposes of the disclosure may feature at least about 1 amino acid, at least about 3 amino acids, at least about 5 amino acids, at least about 10-15 amino acids, or about 15-25 amino acids or more amino acids, of the molecule. There is no critical upper limit to the length of the fragment, which could comprise nearly the full-length of the protein sequence, or the full-length of the protein sequence, or even a fusion protein comprising at least one epitope of the protein.

As used herein, the term “epitope” refers to the site on an antigen or hapten to which specific B cells and/or T cells respond. The term is also used interchangeably with “antigenic determinant” or “antigenic determinant site”. Antibodies that recognize the same epitope can be identified in a simple immunoassay showing the ability of one antibody to block the binding of another antibody to a target antigen.

As used herein, the term “immunological response” to a composition or vaccine refers to the development in the host of a cellular and/or antibody-mediated immune response to a composition or vaccine of interest. Usually, an “immunological response” includes but is not limited to one or more of the following effects: the production of antibodies, B cells, helper T cells, and/or cytotoxic T cells, directed specifically to an antigen or antigens included in the composition or vaccine of interest. The host may display either a therapeutic or protective immunological response so resistance to new infection will be enhanced and/or the clinical severity of the disease reduced. Such protection will be demonstrated by either a reduction or lack of symptoms normally displayed by an infected host, a quicker recovery time and/or a lowered viral titer in the infected host.

As used herein, the term “variant” refers to a substantially similar sequence. For polynucleotides, a variant comprises a deletion and/or addition and/or change of one or more nucleotides at one or more sites within the native polynucleotide and/or a substitution of one or more nucleotides at one or more sites in the native polynucleotide. As used herein, a “native” polynucleotide or polypeptide comprises a naturally occurring nucleotide sequence or an amino acid sequence, respectively. Variants of a particular polynucleotide of the disclosure (e.g., the reference polynucleotide) can also be evaluated by comparison of the percent sequence identity between the polypeptide encoded by a variant polynucleotide and the polypeptide encoded by the reference polynucleotide. “Variant” protein is intended to mean a protein derived from the native protein by deletion or addition of one or more amino acids at one or more sites in the native protein and/or substitution of one or more amino acids at one or more sites in the native protein. Variant proteins encompassed by the present disclosure are biologically active, that is they have the ability to elicit an immune response.

The HLA-DR/HLA-A*0201/hACE2 triple transgenic mouse model referred to herein is a novel susceptible animal model for pre-clinical testing of human COVID-19 vaccine candidates derived from crossing ACE2 transgenic mice with the unique HLA-DR/HLA-A*0201 double transgenic mice. ACE2 transgenic mice are a hACE2 transgenic mouse model expressing human ACE2 receptors in the lung, heart, kidney and intestine (Jackson Laboratory, Bar Harbor, Me.). The HLA-DR/HLA-A*0201 double transgenic mice are “humanized” HLA double transgenic mice expressing Human Leukocyte Antigen HLA-A*0201 class I and HLA DR*0101 class II in place of the corresponding mouse MHC molecules (which are knocked out). The HLA-A*0201 haplotype was chosen because it is highly represented (>50%) in the human population, regardless of race or ethnicity. The HLA-DR/HLA-A*0201/hACE2 triple transgenic mouse model is a “humanized” transgenic mouse model and has three advantages: (1) it is susceptible to human SARS-CoV2 infection; (2) it develops symptoms similar to those seen in COVID-19 in humans; and (3) it develops CD4⁺ T cells and CD8⁺ T cells response to human epitopes. The novel HLA-DR/HLA-A*0201/hACE2 triple transgenic mouse model of the present invention may be used in the pre-clinical testing of safety, immunogenicity and protective efficacy of the human multi-epitope COVID-19 vaccine candidates of the present invention.

As used herein, the terms “treat” or “treatment” or “treating” refers to both therapeutic treatment and prophylactic or preventative measures, wherein the object is to prevent or slow the development of the disease, such as slow down the development of a disorder, or reducing at least one adverse effect or symptom of a condition, disease or disorder, e.g., any disorder characterized by insufficient or undesired organ or tissue function. Treatment is generally “effective” if one or more symptoms or clinical markers are reduced as that term is defined herein. Alternatively, a treatment is “effective” if the progression of a disease is reduced or halted. That is, “treatment” includes not just the improvement of symptoms or decrease of markers of the disease, but also a cessation or slowing of progress or worsening of a symptom that would be expected in absence of treatment. Beneficial or desired clinical results include, but are not limited to, alleviation of one or more symptom(s), diminishment of extent of disease, stabilized (e.g., not worsening) state of disease, delay or slowing of disease progression, amelioration or palliation of the disease state, and remission (whether partial or total), whether detectable or undetectable. “Treatment” can also mean prolonging survival as compared to expected survival if not receiving treatment. “Treatment” also includes ameliorating a disease, lessening the severity of its complications, preventing it from manifesting, preventing it from recurring, merely preventing it from worsening, mitigating an inflammatory response included therein, or a therapeutic effort to affect any of the aforementioned, even if such therapeutic effort is ultimately unsuccessful.

As used herein, the term “carrier” or “pharmaceutically acceptable carrier” or “pharmaceutically acceptable vehicle” refers to any appropriate or useful carrier or vehicle for introducing a composition to a subject. Pharmaceutically acceptable carriers or vehicles may be conventional but are not limited to conventional vehicles. For example, E. W. Martin, Remington's Pharmaceutical Sciences, Mack Publishing Co., Easton, Pa., 15th Edition (1975) and D. B. Troy, ed. Remington: The Science and Practice of Pharmacy, Lippincott Williams & Wilkins, Baltimore Md. and Philadelphia, Pa., 21^stEdition (2006) describe compositions and formulations suitable for pharmaceutical delivery of one or more therapeutic compounds or molecules. Carriers (e.g., pharmaceutical carriers, pharmaceutical vehicles, pharmaceutical compositions, pharmaceutical molecules, etc.) are materials generally known to deliver molecules, proteins, cells and/or drugs and/or other appropriate material into the body. In general, the nature of the carrier will depend on the nature of the composition being delivered as well as the particular mode of administration being employed. In addition to biologically-neutral carriers, pharmaceutical compositions administered may contain minor amounts of non-toxic auxiliary substances, such as wetting or emulsifying agents, preservatives, and pH buffering agents and the like. Patents that describe pharmaceutical carriers include, but are not limited to: U.S. Pat. Nos. 6,667,371; 6,613,355; 6,596,296; 6,413,536; 5,968,543; 4,079,038; 4,093,709; 4,131,648; 4,138,344; 4,180,646; 4,304,767; 4,946,931, the disclosures of which are incorporated in their entirety by reference herein. The carrier may, for example, be solid, liquid (e.g., a solution), foam, a gel, the like, or a combination thereof. In some embodiments, the carrier comprises a biological matrix (e.g., biological fibers, etc.). In some embodiments, the carrier comprises a synthetic matrix (e.g., synthetic fibers, etc.). In certain embodiments, a portion of the carrier may comprise a biological matrix and a portion may comprise synthetic matrix.

As used herein “coronavirus” may refer to a group of related viruses such as but not limited to severe acute respiratory syndrome (SARS), middle east respiratory syndrome (MERS), and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). All the coronaviruses cause respiratory tract infection that range from mild to lethal in mammals. Several non-limiting examples of Coronavirus strains are described herein. In some embodiments, the compositions may protect against any Sarbecoviruses including but not limited to SARS-CoV1 or SARS-CoV2. As used herein, “severe acute respiratory syndrome coronavirus 2 (SARS-CoV2)” is a betacoronavirus that causes Coronavirus Disease 19 (COVID-19).

A “subject” is an individual and includes, but is not limited to, a mammal (e.g., a human, horse, pig, rabbit; dog, sheep, goat, non-human primate, cow, cat, guinea pig, or rodent), a fish, a bird, a reptile or an amphibian. The term does not denote a particular age or sex. Thus, adult and newborn subjects, as well as fetuses, whether male or female, are intended to be included. A “patient” is a subject afflicted with a disease or disorder. The term “patient” includes human and veterinary subjects

The terms “administering” and “administration” refer to methods of providing a pharmaceutical preparation to a subject. Such methods are well known to those skilled in the art and include, but are not limited to, administering the compositions orally, parenterally (e.g., intravenously and subcutaneously), by intramuscular injection, by intraperitoneal injection, intrathecally, transdermally, extracorporeally, topically or the like.

A composition can also be administered by topical intranasal administration (intranasally) or administration by inhalant. As used herein, “topical intranasal administration” means delivery of the compositions into the nose and nasal passages through one or both of the nares and can comprise delivery by a spraying mechanism (device) or droplet mechanism (device), or through aerosolization of the composition. Administration of the compositions by inhalant can be through the nose or mouth via delivery by a spraying or droplet mechanism. As used herein, “an inhaler” can be a spraying device or a droplet device for delivering a composition comprising the vaccine composition, in a pharmaceutically acceptable carrier, to the nasal passages and the upper and/or lower respiratory tracts of a subject. Delivery can also be directly to any area of the respiratory system (e.g., lungs) via intratracheal intubation. The exact amount of the compositions required will vary from subject to subject, depending on the species, age, weight and general condition of the subject, the severity of the disorder being treated, the particular composition used, its mode of administration and the like. Thus, it is not possible to specify an exact amount for every composition. However, an appropriate amount can be determined by one of ordinary skill in the art using only routine experimentation given the teachings herein.

A composition can also be administered by buccal delivery or by sublingual delivery. As used herein “buccal delivery” may refer to a method of administration in which the compound is delivered through the mucosal membranes lining the cheeks. In some embodiment, for a buccal delivery the vaccine composition is placed between the gum and the cheek of a patient. As used herein “sublingual delivery” may refer to a method of administration in which the compound is delivered through the mucosal membrane under the tongue. In some embodiments, for a sublingual delivery the vaccine composition is administered under the tongue of a patient.

Parenteral administration of the composition, if used, is generally characterized by injection. Injectables can be prepared in conventional forms, either as liquid solutions or suspensions, solid forms suitable for solution of suspension in liquid prior to injection, or as emulsions. A more recently revised approach for parenteral administration involves use of a slow release or sustained release system such that a constant dosage is maintained. See, for example, U.S. Pat. No. 3,610,795, which is incorporated by reference herein.

DETAILED DESCRIPTION OF THE INVENTION

Before the present compounds, compositions, and/or methods are disclosed and described, it is to be understood that this invention is not limited to specific synthetic methods or to specific compositions, as such may, of course, vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting. Embodiments of the present invention can be freely combined with each other if they are not mutually exclusive.

Pan-Coronavirus Vaccines

The present invention features preemptive pan-coronavirus vaccines, methods of use, and methods of producing said vaccines, methods of preventing coronavirus infections, etc. The present invention also provides methods of testing said vaccines, e.g., using particular animal models and clinical trials. The vaccine compositions herein can induce efficient and powerful protection against the coronavirus disease or infection, e.g., by inducing the production of antibodies (Abs), CD4+ T helper (Th1) cells, and CD8+ cytotoxic T-cells (CTL).

The vaccine compositions, e.g., the antigens, herein feature multiple large sequences which may comprise multiple conserved epitopes, that helps provide multiple opportunities for the body to develop an immune response for preventing an infection. Further, the vaccines herein may be designed to be effective against past, current, and future coronavirus outbreaks.

The vaccine composition comprises multiple large sequences. In certain embodiments, the large sequences are conserved large sequences, e.g., sequences that are highly conserved among human coronaviruses and/or animal coronaviruses (e.g., coronaviruses isolated from animals susceptible to coronavirus infections).

The present invention describes the identification of conserved large sequences comprising B cell, CD4+ T cell, and CD8+ T cell epitopes. For example, FIG. 1 shows a schematic of the development of a pre-emptive pan coronavirus vaccine featuring multiple conserved large sequences comprising multiple B cell epitopes, multiple conserved CD8⁺ T cell epitopes, and multiple CD4+ T cell epitopes. The large sequences are derived from sequence analysis of many coronaviruses.

Coronaviruses used for determining conserved large sequences may include human SARS-CoVs as well as animal CoVs (e.g. bats, pangolins, civet cats, minks, camels, etc.) as described herein. As an example, FIG. 2A and FIG. 2B show an evolutionary comparison of genome sequences among beta-coronavirus strains isolated from humans and animals. FIG. 2A shows a phylogenetic analysis performed between SARS-CoV-2 strains (obtained from humans (Homo sapiens (black)), along with the animal's SARS-like Coronaviruses genome sequence (SL-CoVs) sequences obtained from bats (Rhinolophus affinis, Rhinolophus malayanus (red)), pangolins (Manis javanica (blue)), civet cats (Paguma larvata (green)), and camels (Camelus dromedarius (Brown)). The included SARS-CoV/MERS-CoV strains are from previous outbreaks (obtained from humans (Urbani, MERS-CoV, OC43, NL63, 229E, HKU1-genotype-B), bats (WIV16, WIV1, YNLF-31C, Rs672, recombinant strains), camel (Camelus dromedarius, (KT368891.1, MN514967.1, KF917527.1, NC_028752.1), and civet (Civet007, A022, 8039)). The human SARS-CoV-2 genome sequences are represented from six continents. FIG. 2B shows an evolutionary analysis performed among the human-SARS-CoV-2 genome sequences reported from six continents and SARS-CoV-2 genome sequences obtained from bats (Rhinolophus affinis, Rhinolophus malayanus), and pangolins (Manis javanica)).

Additionally, other coronaviruses may be used for determining conserved large sequences (including human SARS-CoVs as well as animal CoVs (e.g., bats, pangolins, civet cats, minks, camels, etc.)) that meet the criteria to be classified as “variants of concern” or “variants of interest.” Coronavirus variants that appear to meet one or more of the undermentioned criteria may be labeled “variants of interest” or “variants under investigation” pending verification and validation of these properties. In some embodiments, the criteria may include increased transmissibility, increased morbidity, increased mortality, increased risk of “long COVID”, ability to evade detection by diagnostic tests, decreased susceptibility to antiviral drugs (if and when such drugs are available), decreased susceptibility to neutralizing antibodies, either therapeutic (e.g., convalescent plasma or monoclonal antibodies) or in laboratory experiments, ability to evade natural immunity (e.g., causing reinfections), ability to infect vaccinated individuals, increased risk of particular conditions such as multisystem inflammatory syndrome or long-haul COVID or increased affinity for particular demographic or clinical groups, such as children or immunocompromised individuals. Once validated variants of interest are renamed “variant of concern” by monitoring organizations, such as the CDC.

The conserved large sequences may be derived from structural (e.g., spike glycoprotein, envelope protein, membrane protein, nucleoprotein) or non-structural proteins of the coronaviruses (e.g., any of the 16 NSPs encoded by ORF1a/b).

In some embodiments, the large sequences are each highly conserved among one or a combination of: SARS-CoV-2 human strains, SL-CoVs isolated from bats, SL-CoVs isolated from pangolin, SL-CoVs isolated from civet cats; and MERS strains isolated from camels. For example, in certain embodiments, the large sequences are each highly conserved among one or a combination of: at least 50,000 SARS-CoV-2 human strains, five SL-CoVs isolated from bats, five SL-CoVs isolated from pangolin, three SL-CoVs isolated from civet cats, and four MERS strains isolated from camels. In certain embodiments, the large sequences are each highly conserved among one or a combination of: at least 80,000 SARS-CoV-2 human strains, five SL-CoVs isolated from bats, five SL-CoVs isolated from pangolin, three SL-CoVs isolated from civet cats, and four MERS strains isolated from camels. In certain embodiments, the large sequences are each highly conserved among one or a combination of: at least 50,000 SARS-CoV-2 human strains in circulation during the COVID-19 pandemic, at least one CoV that caused a previous human outbreak, five SL-CoVs isolated from bats, five SL-CoVs isolated from pangolin, three SL-CoVs isolated from civet cats, and four MERS strains isolated from camels. In certain embodiments, the large sequences are each highly conserved among at least 1 SARS-CoV-2 human strain in current circulation, at least one CoV that has caused a previous human outbreak, at least one SL-CoV isolated from bats, at least one SL-CoV isolated from pangolin, at least one SL-CoV isolated from civet cats, and at least one MERS strain isolated from camels. In certain embodiments, the large sequences are each highly conserved among at least 1,000 SARS-CoV-2 human strains in current circulation, at least two CoVs that has caused a previous human outbreak, at least two SL-CoVs isolated from bats, at least two SL-CoVs isolated from pangolin, at least two SL-CoVs isolated from civet cats, and at least two MERS strains isolated from camels. In certain embodiments, the large sequences are each highly conserved among one or a combination of: at least one SARS-CoV-2 human strain in current circulation, at least one CoV that has caused a previous human outbreak, at least one SL-CoV isolated from bats, at least one SL-CoV isolated from pangolin, at least one SL-CoV isolated from civet cats, and at least one MERS strain isolated from camels. The present invention is not limited to the aforementioned coronavirus strains that may be used to identify conserved large sequences.

In certain embodiments, one or more of the conserved large sequences are derived from one or more SARS-CoV-2 human strains or variants in current circulation; one or more coronaviruses that has caused a previous human outbreak; one or more coronaviruses isolated from animals selected from a group consisting of bats, pangolins, civet cats, minks, camels, and other animal receptive to coronaviruses; and/or one or more coronaviruses that cause the common cold. SARS-CoV-2 human strains and variants in current circulation may include the original SARS-CoV-2 strain (SARS-CoV-2 isolate Wuhan-Hu-1), and several variants of SARS-CoV-2 including but not limited to variant B.1.177 (Spain); variant B.1.160 (Australia), variant B.1.1.7 (UK), variant P.1 (Japan/Brazil), variant B.1,351 (South Africa), variant B.1.427 (California), variant B.1.429 (California), variant B.1.258 (Scotland); variant B.1.221 (Belgium/Netherlands); variant 8.1.367 (Norway/France); variant 8.1.1.277 (UK); variant 8.1.1.302 (Sweden); variant 8.1.525 (North America, Europe, Asia, Africa, and Australia); variant B.1.526 (New York), variant S:677H; variant S:677P; B.1.617.2-Delta, variant 8.1.1.529-Omicron (BA.1); sub-variant Omicron (BA.1); sub-variant Omicron (BA.2); sub-variant Omicron (BA.3); sub-variant Omicron (BA.4); sub-variant Omicron (BA.5). The present invention is not limited to the aforementioned variants of SARS-CoV-2 and encompasses variants identified in the future. The one or more coronaviruses that cause the common cold may include but are not limited to strains 229E (alpha coronavirus), NL63 (alpha coronavirus), OC43 (beta coronavirus), HKU1 (beta coronavirus).

As used herein, the term “conserved” refers to a large sequence that is among the most highly conserved large sequences identified in a sequence alignment and analysis. For example, the conserved large sequences may be the 2 most highly conserved sequences identified. In some embodiments, the conserved large sequences may be the 3 most highly conserved sequences identified. In some embodiments, the conserved large sequences may be the 4 most highly conserved sequences identified. In some embodiments, the conserved large sequences may be the 5 most highly conserved sequences identified. In some embodiments, the conserved large sequences may be the 6 most highly conserved sequences identified. In some embodiments, the conserved large sequences may be the 7 most highly conserved sequences identified, in some embodiments, the conserved large sequences may be the 8 most highly conserved sequences identified. In some embodiments, the conserved large sequences may be the 9 most highly conserved sequences identified. In some embodiments, the conserved large sequences may be the 10 most highly conserved sequences identified. In some embodiments, the conserved large sequences may be the 15 most highly conserved sequences identified. In some embodiments, the conserved large sequences may be the 20 most highly conserved sequences identified. In some embodiments, the conserved large sequences may be the 25 most highly conserved sequences identified. In some embodiments, the conserved large sequences may be the 30 most highly conserved sequences identified. In some embodiments, the conserved large sequences may be the 40 most highly conserved sequences identified. In some embodiments, the conserved large sequences may be the 50 most highly conserved sequences identified. In some embodiments, the conserved sequences may be the 50% most highly conserved large sequences identified. In some embodiments, the conserved large sequences may be the 60% most highly conserved sequences identified. In some embodiments, the large conserved sequences may be the 70% most highly conserved sequences identified. In some embodiments, the conserved large sequences may be the 80% most highly conserved sequences identified. In some embodiments, the conserved large sequences may be the 90% most highly conserved sequences identified. In some embodiments, the conserved large sequences may be the 95% most highly conserved sequences identified. In some embodiments, the conserved large sequences may be the 99% most highly conserved sequences identified. The present invention is not limited to the aforementioned thresholds.

FIG. 3A shows an example of a systems biology approach utilized in the present invention.

In some embodiments, the composition comprises one or more large sequences. In some embodiments, the one or more large sequences comprises at least one of one or more conserved coronavirus B-cell target epitopes; one or more conserved coronavirus CD4⁺ T cell target epitopes; and one or more conserved coronavirus CD8⁺ T cell target epitopes

In other embodiments, the vaccine composition comprises two or more large sequences. In some embodiments, the two or more large sequences comprises at least one of one or more conserved coronavirus B-cell target epitopes; one or more conserved coronavirus CD4⁺ T cell target epitopes; and one or more conserved coronavirus CD8⁺ T cell target epitopes

In some embodiments, the large sequences comprise one or more conserved coronavirus B-cell target epitopes and one or more conserved coronavirus CD4⁺ T cell target epitopes. In some embodiments, the large sequences comprise one or more conserved coronavirus B-cell target epitopes and one or more conserved coronavirus CD8⁺ T cell target epitopes. In some embodiments, the large sequences comprise one or more conserved coronavirus CD8⁺ target epitopes and one or more conserved coronavirus CD4⁺ T cell target epitopes. In some embodiments, the large sequences comprise one or more conserved coronavirus CD8⁺ target epitopes. In some embodiments, the large sequences comprise one or more conserved coronavirus CD4⁺ target epitopes. In some embodiments, the large sequences comprise one or more conserved coronavirus B cell target epitopes.

In some embodiments, the vaccine composition comprises one or more conserved coronavirus CDB8⁺ target epitopes. In some embodiments, the vaccine composition comprises one or more conserved coronavirus CD4⁺ target epitopes. In some embodiments, the vaccine composition comprises one or more conserved coronavirus B cell target epitopes.

As will be discussed herein, in certain embodiments, the vaccine composition comprises whole spike protein, one or more coronavirus CD4+ T cell target epitopes; and one or more coronavirus CD8+ T cell target epitopes. In certain embodiments, the vaccine composition comprises at least a portion of the spike protein (e.g., wherein the portion comprises a trimerized SARS-CoV-2 receptor-binding domain (RBD)), one or more coronavirus CD4+ T cell target epitopes; and one or more coronavirus CD8+ T cell target epitopes. In some embodiments, the one or more coronavirus CD4+ T cell target epitopes; and one or more coronavirus CD8+ T cell target epitopes may be in the form of a large sequence.

The large sequences may be each separated by a linker. In certain embodiments, the linker allows for an enzyme to cleave between the large sequences. The present invention is not limited to particular linkers or particular lengths of linkers. As an example, in certain embodiments, one or more large sequences may be separated by a linker 2 amino acids in length or a linker 3 amino acids in length, or a linker 4 amino acids in length, or a linker 5 amino acids in length, or a linker 6 amino acids in length, or a linker 7 amino acids in length, or a linker 8 amino acids in length, or a linker 9 amino acids in length, or a linker 10 amino acids in length. In certain embodiments, one or more large sequences may be separated by a linker from 2 to 10 amino acids in length.

Linkers are well known to one of ordinary skill in the art. Non-limiting examples of linkers include AAY, KK, and GPGPG.

The large sequences may be derived from structural proteins, non-structural proteins, or a combination thereof. For example, structural proteins may include spike proteins (S), envelope proteins (E), membrane proteins (M), or nucleoproteins (N).

In some embodiments, the large sequences are derived from at least one SARS-CoV-2 protein. The SARS-CoV-2 proteins may include ORF1ab protein, Spike glycoprotein, ORF3a protein, Envelope protein, Membrane glycoprotein, ORF6 protein, ORF7a protein, ORF7b protein, ORF8 protein, Nucleocapsid protein, and ORF10 protein. The ORF1ab protein provides nonstructural proteins (Nsp) such as Nsp1, Nsp2, Nsp3 (Papain-like protease), Nsp4, Nsp5 (3C-like protease), Nsp6, Nsp7, Nsp8, Nsp9, Nsp10, Nsp11, Nsp12 (RNA polymerase), Nsp13 (5′ RNA triphosphatase enzyme), Nsp14 (guanosineN7-methyltransferase), Nsp15 (endoribonuclease), and Nsp16 (2′O-ribose-methyltransferase).

The SARS-CoV-2 has a genome length of 29,903 base pairs (bps) ssRNA (SEQ ID NO: 1). Generally, the region between 266-21555 bps codes for ORF1ab polypeptide; the region between 21563-25384 bps codes for one of the structural proteins (spike protein or surface glycoprotein); the region between 25393-26220 bps codes for the ORF3a gene; the region between 26245-26472 bps codes for the envelope protein; the region between 26523-27191 codes for the membrane glycoprotein (or membrane protein); the region between 27202-27387 bps codes for the ORF6 gene: the region between 27394-27759 bps codes for the ORF7a gene; the region between 27894-28259 bps codes for the ORF8 gene; the region between 28274-29533 bps codes for the nucleocapsid phosphoprotein (or the nucleocapsid protein); and the region between 29558-29674 bps codes for the ORF10 gene.

The large sequences may comprise a T-cell epitope restricted to a large number of human class 1 and class 2 HLA haplotypes and not restricted to HLA-0201 for class 1 or HLA-DR for class 2. The conserved large sequences may be restricted to human HLA class 1 and 2 haplotypes. In some embodiments, the conserved epitopes are restricted to cat and dog MHC class 1 and 2 haplotypes.

Large Sequences

The antigen may comprise large sequences, such as conserved large sequences that are highly conserved among human and animal coronaviruses. As used herein, the term large sequence refers to a sequence having at least 25 amino acids or at least 75 nucleotides. The large sequences comprise epitopes, such as the conserved epitopes described herein.

In some embodiments, the large sequence has at least 75 nt. In some embodiments, the large sequence has at least 150 nt. In some embodiments, the large sequence has at least 200 nt. In some embodiments, the large sequence has at least 250 nt. In some embodiments, the large sequence has at least 300 nt. In some embodiments, the large sequence has at least 400 nt. In some embodiments, the large sequence has at least 500 nt. In some embodiments, the large sequence has at least 600 nt. In some embodiments, the large sequence has at least 700 nt. In some embodiments, the large sequence has at least 800 nt. In some embodiments, the large sequence has at least 900 nt. In some embodiments, the large sequence has at least 1000 nt. In some embodiments, the large sequence has at least 1500 nt. In some embodiments, the large sequence has at least 2000 nt. In some embodiments, the large sequence has at least 2500 nt. In some embodiments, the large sequence has at least 3000 nt. In some embodiments, the large sequence has at least 3500 nt. In some embodiments, the large sequence has at least 4000 nt. In some embodiments, the large sequence has at least 4500 nt. In some embodiments, the large sequence has at least 5000 nt.

In order to identify the conserved large sequences, sequence alignments and analysis were performed as described herein as well as below.

Sequence comparison among SARS-CoV-2 and previous coronavirus strains: Sequence homology analysis we performed and compare the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) isolate Wuhan-Hu-1, to complete genome with sequences of SARS-CoV-2 variants, common cold corona virus strains (HKU1 genotype B, CoV-OC43, CoV-NL63, and CoV-229E), SARS-CoV-Urbani, MERS and coronavirus strains from bats (Rhinolophus affinis and R. malayanus), pangolin (Manis javanica), civet cats (Paguma larvata), and camel (Camelus dromedarius and C. bactrianus).

The human SARS-CoV-2 variant genome sequences were retrieved from the GISAID database, representing major Variants of Concern which are known for their high degree of transmissibility and pathogenicity. The sequences used in this study are 20A.EU1 from Spain (EPI_ISL_691726-hCoV-19-VOC-20A.EU1), 20A.EU2 from Australia (EPI_ISL_418799-hCoV-19-VOC-20A.EU2), B.1.1.7 from England (EPI_ISL_581117-hCoV-19-VOC-B.1.1.7), B.1.351 from South Africa (EPI_ISL_660615-hCoV-19-VOC-B.1.351), P.1 from Brazil (EPI_ISL_581117-hCoV-19-VOC-P.1), CAL.20C from California (EPI_ISL_730092-hCoV-19-VOC-B.1.427/B.1.429), B.1.258 from Scotland (EPI_ISL_858559-hCoV-19-VOC-B.1.258), B.1.221 from Belgium/Netherlands (EPI_ISL_734790-hCoV-19-VOC-B.1.221), B.1.367 from Norway/France (EPI_ISL_541518-hCoV-19-VOC-B.1.367), B.1.1.277 from Netherlands/Denmark/UK (EPI_ISL_500783-hCoV-19-VOC-B.1.1.277), B.1.1.302 from Sweden (EPI_ISL_717929-hCoV-19-VOC-B.1.1.302). Similarly, HKU1 genotype B (AY884001), CoV-OC43 (KF923903), CoV-NL63 (NC_005831), and CoV-229E (KY983587), SARS-CoV-Urbani (AY278741.1), MERS (NC_019843).

Bat CoV strains used in this analysis include strains RaTG13 (MN996532.2), Rs672/2006 (FJ588686.1), YNLF_31C (KP886808.1), WIV1 (KF367457.1), WIV16 (KT444582.1), ZXC21 (MG772934.1), RmYN02 (EPI_ISL_412977), bat-RmYN01 (EPI_ISL_412976), MERS-Bat-CoV/P. khulii/Italy/206645-63/2011 (MG596803.1). More-so, five genome sequences representing Pangolin (MT040333.1-PCoV_GX-P4L, MT040334.1-PCoV_GX-P1E, MT040335.1-PCoV_GX-P5L, MT040336.1-PCoV_GX-P5E, MT072864.1-PCoV_GX-P2V, MT121216.1-PCoV-MP789), three Civet cat specific genome sequences (AY572034.1, AY686864.1, AY686863.1), and four CoV sequences from camels (NC_028752.1, KF917527.1, MN514967.1, KT368891.1) were included in this sequence homology analysis aimed at evaluating the most conserved regions in different structural and non-structural proteins in CoV genome. These sequences were obtained either from National Center for Biotechnology Information (NCBI) or Global initiative on sharing all influenza data (GISAID). For phylogenetic analyses, SARS-CoV-2 full-genome sequences were aligned with CLUSTAL W using MEGAX. All the SARS-CoV-2 sequences were compared to existing genomes using online NCBI BLAST.

Determination of SARS-COV-2 Sequence Conservation: Each Wuhan-Hu-1 (GeneBank: NC_045512.2) specific structural (Spike glycoprotein (YP_009724390.1), Membrane protein (YP_009724393.1), Envelope protein (YP_009724392.1), Nucleocapsid phosphoprotein (YP_009724397.2)), and non-structural proteins (ORF1a/b polyprotein (YP_009724389.1), ORF3a (YP_009724391.1), ORF6 (YP_009724394.1), ORF7a (YP_009724395.1), ORF7b (YP_009725318.1), ORF8 (YP_009724396.1), and ORF10 (YP_009725255.1)) protein sequences were compared against the consensus protein sequences from SARSCoV and MERS-CoV and the protein sequences from closest relative cross species CoV strains using the Nucleotide BLAST (blastn) algorithm to compute the pairwise identity between Wuhan-Hu-1 proteins and their comparison target.

Further as the present invention is interested in the highly similar sequences across CoV strains, megablast was performed. For each of the queried sequences, Query coverage, E value. Percent identity were determined. The queried homology obtained against one bat CoV strain RmYN01, which was found earlier to be phylogenetically less similar to SARS-CoV-2, but has more genetic similarities with SARS-CoV-Urbani was taken as a standard to ascertain the homologous sequences across CoV strains. The strategy was helpful to find out how genetically more conserved regions among different CoVs. This sequence has a query coverage of 59%, and a percent identity of 78.73% when compared against the SARS-CoV-2 genome sequence. It has five matched regions which further showed sequence homology among other CoVs as well. Matched region 1 spanned between 1 bp-1580 bp (fragment) showed sequence homology with nsp1 (leader protein), nsp2, and nsp3, whereas matched region 2 spanned between 3547 bp-7096 bp (fragment 2) showed sequence homology with multiple subunits of ORF1a/b like 3CLpro, nsp6, nsp7, nsp8, nsp9, nsp10, RNA dependent RNA polymerase, helicase, nsp14, nsp15, and nsp16. Interestingly, a major region spanning in the non-annotated region of the ORF1a/b between 17472 bp-21156 bp (fragment 3) also showed sequence identity. The fourth stretch of sequence identity spanned through 22584 bp-24682 bp (fragment 4) covering a section of the Spike glycoprotein, that importantly covers the major Receptor Binding Domain in the SARS-CoV-2 as well. The last segment of the homologous sequence showed percent identity with regions specific to the ORF3a, Envelope protein, Membrane protein, ORF6, and ORF7a (26193 bp-27421 bp; fragment 5).

In some embodiments, five fragments from the SARS-CoV-2 Wuhan Strain were found to be highly conserved (1 bp-1580 bp (fragment 1), 3547 bp-12830 bp (fragment 2), 17472 bp-21156 bp (fragment 3), 22584 bp-24682 bp (fragment 4), and 26193 bp-27421 bp (fragment 5). Next, each fragment underwent another round of sequence homology analysis.

In some embodiments, the vaccine composition comprises one large sequence. In some embodiments, the vaccine composition comprises one or more large sequences. In some embodiments, the vaccine composition comprises two or more large sequences. In some embodiments, the vaccine composition comprises three or more large sequences. In some embodiments, the vaccine composition comprises four or more large sequences. In some embodiments, the vaccine composition comprises five or more large sequences, e.g., 5, 6, 7, 8, etc.

In some embodiments, the large sequences are derived from a whole protein sequence expressed by SARS-CoV-2. In other embodiments, large sequences are derived from a partial protein sequence expressed by SARS-CoV-2. In some embodiments, the large sequence of said proteins comprise B cell epitopes and T-cell epitopes that are restricted to a large number, e.g., from 3 to 10, different haplotypes that encompass 100% of the population regardless of race and ethnicity) of human class 1 and class 2 HLA haplotypes, so they are not restricted only to HLA-0201 for class 1 or HLA-DR1 for class 2.

As previously discussed, the large sequences may be highly conserved among human and animal coronaviruses. In some embodiments, the large sequences are derived from one or a combination of: one or more SARS-CoV-2 human strains or variants in current circulation; one or more coronaviruses that has caused a previous human outbreak; one or more coronaviruses isolated from animals selected from a group consisting of bats, pangolins, civet cats, minks, camels, and other animal receptive to coronaviruses; and/or one or more coronaviruses that cause the common cold.

As previously discussed, the SARS-CoV-2 human strains or variants in current circulation may include variant 6.1.177; variant B.1.160, variant 6.1.1.7 (UK), variant P.1 (Japan/Brazil), variant B.1.351 (South Africa), variant 8.1.427 (California), variant 6.1.429 (California), variant B.1.258; variant B.1.221; variant B.1.367; variant B.1.1.277; variant B.1.1.302; variant B.1.525; variant 6.1.526, variant S:677H; variant S:677P; B.1.617.2-Delta, variant B.1.1.529-Omicron (BA.1); sub-variant Omicron (BA.1); sub-variant Omicron (BA.2); sub-variant Omicron (BA.3); sub-variant Omicron (BA.4); sub-variant Omicron (BA.5). In some embodiments, the one or more coronaviruses that cause the common cold are selected from 229E alpha coronavirus, NL63 alpha coronavirus, OC43 beta coronavirus, and HKU1 beta coronavirus.

The large sequence(s) may be derived from structural proteins, non-structural proteins, or a combination thereof. The large sequence(s) may be selected from ORF1ab protein, Spike glycoprotein (e.g., the RBD), ORF3a protein, Envelope protein, Membrane glycoprotein, ORF6 protein, ORF7a protein, ORF7b protein, ORF8 protein, Nucleocapsid protein, and/or an ORF10 protein. Note the ORF1ab protein comprises nonstructural protein (Nsp) 1, Nsp2, Nsp3, Nsp4, Nsp5, Nsp6, Nsp7, Nsp8, Nsp9, Nsp10, Nsp11, Nsp12, Nsp13, Nsp14, Nsp15 and Nsp16.

In some embodiments, a large sequence comprises conserved fragments from over 150,000 CoV strains circulating in the majority of countries around the world (Table 1, FIG. 4). In some embodiments, fragment 1 comprises the base pairs 1-1580. In some embodiments, fragment 1 may comprise the proteins Nsp1, Nsp2, and Nsp3 as well as unannotated regions (FIG. 5). In some embodiments, fragment 2, comprises the base pairs 3547-12830. In some embodiments, fragment 2 may comprise the proteins Nsp5, Nsp6, Nsp7, Nsp8, Nsp9, Nsp10, Nsp11, Nsp12, Nsp13, Nsp14, Nsp15, Nsp16, as well as unannotated regions (FIG. 6). In some embodiments, fragment 3 comprises the base pairs 17472-21156. In some embodiments, fragment 3 comprises unannotated regions (FIG. 7). In some embodiments, fragment 4 comprises the base pairs 22584-24682. In some embodiments, fragment 4 comprises the spike glycoprotein (FIG. 8). In some embodiments, fragment 5 comprises the base pairs 26193-27421. In some embodiments, fragment 5 comprises the proteins ORF3a, Envelope (E), Membrane (M), ORF6, ORF7a, as well as unannotated regions (FIG. 9).

TABLE 1

SEQ

Fragment

ID

No:
Proteins
Sequence
NO:

1
Nspt
GACGTGCTAGTACGTGGCTTCGGGGACTCTGTGGAAGAGGCCCTA
182

(1 bp-
Nsp2,
TCGGAGGCACGTGAACATCTTAAAAGTGGCACTTGTGGCATAGTAG

1580 bp)
Nsp3
AGCTGGAAAAAGGCGTATTGCCTCAGCTTGAACAGCCCTATGTGTT

CATTAAACGATCTGACGCCCAGGGCACTGGTCATGGCCACAAGGT

CTGTGAGCTAGTTGCTGAATTGGATGGCGTTCAGTTCGGTCGTAGC

GGTATAACACTGGGAGTACTCGTGCCACACGTTGGCGAGACCCCA

ATTGCATACCGCACTGTTCTTCTTCGTAAGAATGGTAATAAGGGAGC

CGGTGGCCATAGCTTTGGCATCGATCTAAAGTCATATGACTTAGGT

GACGAGCTTGGCACTGATCCCATTGAAGATTATGAACAAAACTGGA

ACACTAAACATGGCAGTGGTGCCCTTCGTGAACTCACTCGTGAGCT

CAATGGAGGAGTAGTTACTCGCTATGTCGACAACAATTTCTGTGGC

CCAGATGGCTACCCCCTTGAATGCATTAAAGACCTTCTCGCTCGTG

CGGGCAAGTCAATGTGCACTCTTTCTGAACAACTTGATTATCGAG

TCGAAGAGAGGTGTCTACTGCTGTCGTGAACATGAGCATGAAATTG

CTTGGTTTACCGAACGCTCTGAAAAGAGCTATGAGCACCAGACACC

CTTCGAGATCAAGAGTGCCAAGAAATTTGACACTTTCAAAGGGGAA

TGCCCAAAGTTTGTATTTCCTCTCAATTCTAGAGTCAAAGTCATTCA

ACCACGTGTTGAAAAGAAAAAGACTGAAGGTTTCATGGGGCGTATA

CGCTCTGTGTACCCTGTTGCATCCCCTGGGGATTGTAACGATATGC

ACTTGTCTACCTTGATGAAATGTAATCATTGTGATGAAGTTTCATGG

CAGACGTGCGACTTTCTCAAAGCCACTTGTGAACAATGTGGCACTG

AAAACTTAGTCTGTGAAGGACCCACTACATGTGGATACCTACCTACT

AATGCTGTACTTAAAATGCCTTGTCCTGCTTGTCAAGATCCAGAGAT

TGGACCTGAGCATAGTGTTGCAGACTATCACAACCACTCAAACATT

GAAACTCGACTCCGCAAGGGAGGTAGGACTAAATGTTTTGGTGGGT

GTGTGTTTGCCTACGTTGGCTGCTATAACAAGCGTGCCTACTGGGT

TCCTCGTGCTAGTGCCGATATTGGTGCAAACCATACTGGCATTACT

GGAGACAATGTGGAGACTTTAAATGAAGATCTCCTGGAGATACTGC

ATCGTGAACGTGTTAATGTTAACATTGTTGGCGATTTTCAGTTGAAT

GAAGAGGTTGCTATTATTCTAGCATCTTTCTCTGCTTCTACTAGTGC

CTTTATTGACACTGTAAAGGGCCTTGACTACAAGACCTTCAAAGCCA

TTGTTGAATCCTGTGGAAACTACAAAGTTACCAAAGGAAAACCTGTC

CAAGGAGCTTGGAACATTGGCCAGCAAAAATCTATTTTGACACCGC

TGTGTGGTTTTCCATCACAGGCTGCCAGTGTCATTAGATCAATCTTT

TCTCGCAC

2
Nsp5_,
AAAATTAAGGCTTGCATCGAAGAGGTCACTACAACACTGGAAGAGA
183

(2547 bp-
Nsp6,
CTAAGTTTCTTACCAATAAGTTGCTTCTTTTTGCTGATATCAGCGGTA

12830 bp)
Nsp7,
AACTTTACCAAGATTCTCAGAATATGCTTAGAGGTGAGGACGTGTCT

Nsp8,
TTCCTTGAGAGAGATGCGCCTTACATGGTAGGTGATGTTATCAATA

Nsp9,
GTGGTGATATTACCTGCGTTGTAATACCTTCTAAGAAGGCTGGTGG

Nsp10,
TACTACAGAAATGCTTGCAAGAGCATTGAAGAAAGTGCCARTTGAT

Nsp11,
GAGTATATAACCACATAYCCTGGWCAAGGWTGTGCTGGTTATACAC

Nsp12,
TTGAKGAAGCTARGACTGCTCTTAARAARTGCAAATCTGCAYTKTAY

Nsp13,
GTKTTACCTTCAGAATCACCTAATGCTAAGGAAGAGATTCTAGGAAC

Nsp14,
CGTATCTTGGAATTTGAGAGAAATGCTTGCTCACGCTGAAGAGACA

Nsp15,
AGAAAATTAATGCCTATCTGCATGGATGTCAGAGCCATAATGGCCA

Nsp16
CCATCCAACGCAAGTACAAAGGAATTAAAATTCAAGAAGGCATCGTT

GACTATGGTGTCCGATTCTTCTTTTATACTAGTAAAGAGCCTGTAGC

TTCTATTATTACGAAGCTGAACTCTCTAAATGAGCCACTTGTCACAA

TGCCAATTGGTTATGTGACACATGGTTTTAATCTTGAAGAGGCTGCG

CGCTGTATGCGTTCTCTTAAAGCTCCTGCCGTAGTGTCAGTATCATC

ACCAGATGCYGTTACTACATATAATGGATACCTCACTTCGTCATCAA

AGACATCTGAGGAGCACTTTGTGGAAACAGTTTCTTTGGCTGGCTC

TTACAGAGATTGGTCCTATTCAGGACAGCGTACAGAGTTAGGTGTT

GAATTTCTTAAGCGTGGTGACAAAATTGTGTACCACACTTTGGAGAG

CCCCGTCAAGTTCCATCTTGACGGTGAGGTTCTTCCACTTGACAAA

TTAAAGAGTCTCTTATCCCTACGGGAGGTTAAGACTATAAAAGTGTT

CACAACTGTGGACAATACTAATCTCCACACACATCTTGTGGATATGT

CTATGACATATGGACAGCAGTTTGGTCCAACATATTTGGATGGTGCT

GATGTTACAAAAATTAAACCTCATGTAAATCATGAGGGTAAGACTTT

CTTTGTATTACCTAGTGATGACACACTACGTAGTGAAGCTTTTGAGT

ACTACCACGCTCTTGATGAGAGTTTCCTTGGTAGATACATGTCTGCT

TTAAACCACACAAAGAAATGGAAATTCCCTCAAGTTGGTGGTTTGAC

TTCCATTAAGTGGGCTGATAACAATTGTTATTTGTCTAGTGTTTTATT

AGCACTTCAACAAATTGAAGTTAAATTTAATGCCCCAGCACTACAAG

AAGCTTACTATAGAGCTCGTGCTGGTGATGCTGCTAATTTTTGTGCA

CTTATACTCGCTTACAGTAATAAAACTGTTGGCGAGCTGGGTGATGT

CAGAGAAACTATGGCCCATCTTTTACAGCATGCTAATTTGGAATCTG

CAAAGCGAGTTCTTAATGTGGTGTGTAAACATTGCGGCCAGAAAAC

TACTACCTTAACGGGTGTAGAGGCTGTGATGTACATGGGTACTCTG

TCTTATGATAATCTTAAGACAGGTGTTTCTGTTCCATGTGTGTGTGG

TCGTGACGCTACACAATATTTAGTACAACAAGAGTCTTCTTTTGTTAT

GATGTCCGCACCACCTGCTGAATATAAATTACAGCAAGGTACATTCT

TATGTGCAAATGAATACACTGGTAATTATCAGTGTGGTCATTACACT

CATATAACTGCTAAGGAGACCCTCTATCGTATTGATGGAGCTCACCT

TACAAAGATGTCAGAGTATAAAGGGCCAGTGACTGATGTGTTCTAC

AAGGAAACATCTTACACTACAACCATCAAGCCTGTGTCATATAAACT

CGATGGAGTTACTTACACAGAGATTGAACCAAAATTGGATGGGTATT

ATAAAAAGGATAATGCTTACTATACGGAGCAGCCTATAGACCTTGTA

CCAACTCAACCACTACCAAATGCGAGTTTTGATAATTTCAAACTCAC

ATGTTCTAATATAAAATTCGCTGATGATTTAAATCAAATGACAGGCTT

CACAAAGCCAGCTTCACGAGAGCTATCTGTCACATTCTTTCCAGACT

TGAATGGCGATGTAGTGGCTATTGACTATAGACACTACTCAGCGAG

TTTCAAGAAAGGTGCTAGATTACTGCATAAGCCAATTGTTTGGCATA

TCAATCAGGCTACAACCAAGACAACGTTCAGACCAAACACTTGGTG

TTTACGTTGTCTTTGGAGTACAAAACCAGTAGATACTTCAAATTCATT

TGAAGTTCTGGCAGTAGAAGACACACAAGGAATGGACAATCTTGCT

TGTGAAAGTCAAAGACCCACCTCTGAAGAAGTAGTGGAAAATCCTA

CCATACAGAAGGAAGTCATAGAGTGTGACGTGAAAACTACCGAAGT

TGTAGGCAATGTCATACTTAAACCATCAGATGAAGGTGTTAAAGTAA

CACAAGAGTTAGGGCATGAGGATCTTATGGCTGCCTATGTGGAAAA

TACAAGCATTACCATTAAGAAACCTAATGAGCTTTCATTAGCCTTAG

GTTTAAAAACAATTGCCACTCATGGTATTGCTGCAATTAACAGTGTT

CCGTGGAGTAAAATTTTGGCTTATGTCAGACCATTCCTAGGACGAA

CAGCAATCACAACATCAAACTGTGCTAAGAGATTAGTACAGCGTGT

ATTTAACAACTACATGCCCTATGTGCTTACATTATTGTTCCAATTGTG

TAGTTTTACCAAAAGTACAAATTCTAGAATTAGAGCTTCACTACCTAC

GACTATTGCTAAAAATAGTGTTAAGGGTGTTGCTAAATTATGTTTGG

ATGCTGGCATCAATTATGTAAAGTCACCCAAATTTTCTAAATTGTTCA

CTATTGCAATGTGGCTATTATTGTTAAGCATTTGCTTAGGTTCACTAA

TCTATGTAACTGCAGCTTTAGGTGTATTATTGTCCAACTTTGGAGCT

CCTTCCTATTGTAGTGGCGTTAGAGAATCGTATCTCAATTCCTCTAA

TGTTACTACTATGGACTTCTGTGAAGGTTCTTTTCCTTGCAGCGTTT

GTTTAAGTGGATTAGACTCGCTTGATTCCTATCCAGCTCTTGAAACC

ATACAGGTAACGATTTCATCGTATAAGCTAGACTTGACAATTTTAGG

TCTGGCTGCTGAGTGGTTTTTGGCATATATGTTGTTCACAAAATTCT

TTTATTTATTAGGTCTTTCAGCTATAATGCAGGTGTTCTTTGGCTATT

TTGCTAGTCATTTCATCAGCAATTCTTGGCTTATGTGGTTTATCATTA

GTATCGTACAAATGGCACCCGTTTCCGCAATGGTTAGGATGTACAT

TTTCGTTGCTTCTTTCTACTACATATGGAAGAGCTATGTTCATATTAT

GGATGGTTGTACTTCATCTACTTGCATGATGTGCT

4
spike
TACCAAGCTACTAGAGTAGTGGTACTTTCATTTGAGCTTCTAAATGC
184

(22584 bp-
glyco-
ACCTGCCACAGTGTGTGGACCAAAATTGTCCACATCACTAATTAAGA

24683 bp)
protein
ACCAGTGTGTCAATTTTAATTTCAATGGACTCAAGGGTACTGGTGTG

TTGACTGACTCGTCCAAAAAGTTTCAGTCTTTTCAACAATTTGGAAG

GGATGCATCTGATTTTACTGACTCAGTACGCGACCCTCAGACACTT

CAAATACTTGACATTTCACCATGTTCATTTGGTGGTGTGAGTGTAAT

AACACCAGGAACAAATGCTTCATCTGAAGTAGCCGTTCTATACCAA

GATGTAAACTGCACTGATGTTCCCACGGCCATACGTGCTGACCAAC

TCACACCTGCTTGGCGTGTTTACTCTGCTGGAGTAAATGTGTTTCAA

ACTCAGGCTGGCTGTTTAATAGGAGCGGAACATGTCAATGCTTCAT

ATGAGTGTGACATTCCCATTGGTGCAGGCATTTGTGCTAGTTACCAT

ACAGCTTCCCTTTTACGTAATACAGGCCAGAAATCAATTGTGGCCTA

TACTATGTCACTTGGTGCTGAAAACTCAATTGCTTATGCTAATAACT

CAATTGCCATACCTACAAATTTTTCAATCAGTGTCACAACTGAAGTG

ATGCCTGTTTCAATGGCTAAGACATCAGTAGATTGTACAATGTACAT

CTGTGGTGACTCTCAGGAGTGCAGCAACTTACTACTTCAGTATGGT

AGCTTTTGCACACAATTAAATCGTGCCCTTTCAGGCATTGCTGTTGA

ACAGGACAAAAACACTCAAGAGGTTTTTGCCCAAGTTAAACAAATGT

ATAAGACACCAGCCATAAAAGATTTTGGTGGCTTTAATTTCTCACAA

ATATTGCCTGACCCTTCTAAGCCAACAAAAAGATCATTTATTGAGGA

TTTACTCTTCAACAAAGTGACTCTCGCTGATGCTGGCTTTATGAAGC

AATACGGCGAATGCCTAGGCGATATTAGTGCTAGAGATCTCATTTG

TGCGCAGAAGTTCAATGGACTCACTGTCCTTCCACCTCTACTCACG

GATGAAATGATTGCTGCTTACACCGCCGCTCTTGTCAGCGGTACTG

CTACTGCTGGTTGGACATTTGGTGCAGGTGCTGCTCTACAAATACC

TTTTGCTATGCAAATGGCTTATAGGTTCAATGGCATTGGAGTTACTC

AAAATGTTCTCTATGAGAACCAGAAGCAGATCGCTAACCAATTTAAC

AAGGCGATCAGTCAAATTCAAGAATCACTTACTACTACTTCAACTGC

ATTGGGCAAGCTGCAAGACGTCGTCAACCAGAATGCTCAAGCATTG

AACACACTTGTTAAACAACTAAGTTCTAACTTTGGTGCAATTTCAAGT

GTTTTAAATGACATTCTGTCTCGACTYGACAAAGTTGAGGCTGAAGT

GCAAATTGATAGGTTGATTACTGGCAGATTACAAAGCCTTCAGACCT

ATGTAACACAACAACTAATCAGAGCTGCTGAAATCAGAGCTTCTGC

CAATCTTGCTGCCACTAAGATGTCCGAGTGTGTTCTTGGACAATCAA

AAAGAGTTGACTTTTGTGGAAAAGGCTATCATCTTATGTCTTTCCCT

CAAGCAGCCCCACATGGTGTCGTCTTCTTACATGTCACATACGTGC

CATCGCAAGAAAGAAACTTCACCACTGCCCCAGCAATCTGCCATCA

AGGCAAGGCACACTTCCCTCGTGAAGGTGTTTTTGTATCTAATGGC

ACTTCTTGGTTTATCACACAGAGGAACTTCTTCACCACAAATAATT

ACAACAGACAATACATTTGTCTCTGGAAATTGTGATGTCGTTATTGG

CATCATCAACAATACTGTTTATGATCCTCTGCAACCTGAGCTTGACT

CATTTAAAGAAGAGCTGGACAAGTACTTCAAAAACCACACGTCACCT

GATGTRGATCTTGGCGACATCTCAGGCATTAATGCTTCAGTCGTCA

ATATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAAAATCTA

AATGAATCGCTCATCGATCTTCAAGAACTTGGAAAATATGAGCA

5
ORF3a,
CAGTAACACTTGCTTGCTTTGTGCTTGCTGCTGTTTACAGAATTAAT
185

(26193 bp-
Envelope
TGGGTGACTGGCGGAATTGCRATTGCAATGGCTTGTATTGTAGGCT

27421 bp)
(E),
TGATGTGGCTTAGCTACTTCRTTGCTTCTTTCAGGCTGTTTGCGCGC

Membrane
ACCCGCTCWATGTGGTCATTCAACCCAGAAACYAACATTCTTCTCA

(M), ORF6,
ATGTGCCTCTTCGRGGRACAATYTTGACCAGACCGCTCATGGARAG

ORF7a
TGAACTTGTCATTGGTGCTGTGATCATTCGTGGTCACCTGCGAATG

GCTGGACACTCYCTWGGGCGCTGTGACATTAAGGACCTGCCAAAA

GAGATCACTGTGGCTACATCACGAACGCTTTCTTATTACAAATTAGG

AGCTTCGCAGCGTGTAGGCACTGACTCAGGTTTTGCTGCATACAAC

CGCTACCGTATTGGAAACTACAAATTAAATACAGACCACGCCGGTA

GCAACGACAATATTGCTTTGCTAGTACAGTAAGTGACAACAGATGTT

TCATCTAGTTGACTTCCAGGTTACAATAGCGGAGATATTGATTATCA

TTATGAGGACTTTCAGGATTGCCATCTGGAATCTTGATGTAATAATA

AGTTCAATAGTGAGACAATTATTTAAGCCTCTAACTAAGAAGAATTAT

TCTGAGTTAGATGATGAAGAACYTATGGAGATTGATTATCCATAAAA

CGAACATGAAAATTATCCTCTTCCTGACTTTGATTTCACTTGCATTTT

GTGAGTTATATCATTATCAGGAGTGTGTTAGAGGTACAACTGTACTA

TTAAAAGAACCTTGCCCATCRGGAACGTACGAGGGCAATTCACCAT

TTCACCCTCTTGCTGACAACAAATTTGCACTAACTTGCATTAGCACA

CATTTTGCTTTTGCTTGTGCTGACGGTACTCGACATACCTATCAGCT

TCGTGCAAGATCAGTTTCTCCAAAACTCTTCATCAGGCAAGAGGAAT

TTCATCAAGAGCTCTATTCACCACTTTTTCTCATTGTTGCCGCTCTA

GTATTTATAATACTTTGCTTCACCATTAAGAGAAAGACCGAATGAGT

GAGCTCACTTTAATTGACTTCTATTTGTGCTTTAGCCTTTCTGCTA

TTCCTTGTTTTAATAATGCTCATCATATTTTGGTTCTCCTTGGAGATT

CAAGATTCTGAAGAGCCATGTCCAAAAGTCTAAACGAACATGAAACT

TCTCATTGTTTT

6
Nsp2
ATGGCATACACTCGCTATGTCGATAACAACTTCTGTGGCCCTGATG
360

GCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAA

AGCTTCATGCACTTTGTCCGAACAACTGGACTTTATTGACACTAAGA

GGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTA

CACGGAACGTTCTGAAAAGAGCTATGAATTGCAGACACCTTTGAA

ATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTCCAAA

TTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGT

TGAAAAGAAAAAGCTTGATGGCTTTATGGGTAGAATTCGATCTGTCT

ATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACT

CTCATGAAGTGTGATCATTGTGGTGAAACTTCATGGCAGACGGGCG

ATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACT

AAAGAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGT

TAAAATTTATTGTCCAGCATGTCACAATTCAGAAGTAGGACCTGAGC

ATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTT

CGTAAGGGTGGTCGCACTATTGCCTTTGGAGGCTGTGTGTTCTCTT

ATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAG

CGCTAACATAGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCC

GAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAAAGT

CAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCA

TTATTTTGGCATCTTTTTCTGCTTCCACAAGTGCTTTTGTGGAAACTG

TGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGT

GGTAATTTTAAAGTTACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAA

TATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCAT

CAGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGA

AACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATAACAA

TACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATG

ATGTTCACATCTGATTTGGCTACTAACAATCTAGTTGTAATGGCCTA

CATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAAC

ATCTTTGGCACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCT

TGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACGGTTGG

GAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGG

ACAAATTGTCACCTGTGCAAAGGAAATTAAGGAGAGTGTTCAGACA

TTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATC

ATTATTGGTGGAGCTAAACTTAAAGCCTTGAATTTAGGTGAAACATT

TGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGA

GAAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTAT

CTTCTTAGAGGGAGAAACACTTCCCACAGAAGTGTTAACAGAGGAA

GTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAG

TGAAGCTGTTGAAGCTCCATTGGTTGGTACACCAGTTTGTATTAACG

GGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCT

TGCACCTAATATGATGGTAACAAACAATACCTTCACACTCAAAGGCG

GTTGATGA

7
Nsp2
ATGGCCTATACCAGGTACGTGGATAATAATTTCTGTGGGCCTGACG
361

(codon
GCTACCCTCTGGAATGCATCAAAGACCTGTTGGCTAGGGCAGGAAA

optimized)
AGCTTCATGCACCCTGTCTGAGCAACTTGACTTCATTGACACAAAG

CGGGGAGTCTACTGCTGCCGCGAACATGAGCACGAGATTGCTTGG

TACACAGAGCGAAGCGAAAAATCTTATGAACTGCAGACACCCTTTG

AAATCAAGTTAGCAAAGAAGTTCGACACCTTTAATGGCGAGTGCCC

AAACTTTGTGTTCCCTCTGAATTCGATCATTAAAACCATCCAGCCTA

GAGTAGAAAAAAAAAAGCTCGATGGTTTCATGGGCCGCATAAGATC

TGTGTATCCTGTGGCTAGCCCCAACGAGTGTAACCAGATGTGTTTG

TCAACCCTGATGAAATGTGATCACTGTGGAGAAACGAGTTGGCAGA

CTGGAGACTTTGTTAAAGCTACTTGCGAATTCTGCGGGACAGAGAA

CCTCACCAAGGAGGGAGCAACCACGTGCGGTTACCTGCCCCAAAA

TGCTGTAGTCAAAATCTACTGTCCGGCATGCCATAACTCAGAGGTT

GGGCCAGAGCATAGCTTGGCAGAGTACCATAATGAATCGGGACTTA

AAACAATCCTGCGGAAGGGTGGCCGTACGATTGCCTTTGGAGGGT

GCGTCAGCTATGTGGGCTGCCACAACAAGTGCGCCTACTGGGT

GCCCAGAGCCAGTGCCAACATTGGATGTAACCACACAGGCGTCGT

TGGGGAGGGCAGTGAGGGCTTGAATGACAATCTTCTGGAGATTCTA

CAAAAGGAAAAGGTCAACATCAACATAGTTGGGGATTTCAAGCTGA

ACGAGGAGATTGCCATCATTTTAGCGTCCTTTTCTGCCTCAACAAGT

GCCTTCGTGGAAACTGTGAAAGGGCTTGACTACAAGGCATTTAAGC

AGATCGTGGAGTCCTGTGGCAACTTTAAAGTGACTAAGGGCAAGGC

CAAAAAGGGGGCCTGGAATATAGGCGAACAGAAGTCCATCCTGAG

CCCTCTCTATGCTTTTGCTAGTGAAGCTGCCCGCGTTGTCCGGTCT

ATCTTCAGCCGAACTTTGGAGACTGCTCAGAACTCTGTCAGAGTCC

TGCAGAAGGCAGCCATTACCATACTTGATGGGATATCTCAATACAG

CCTCCGTCTGATTGATGCCATGATGTTTACTTCAGATCTCGCAACCA

ACAATCTCGTTGTGATGGCTTATATCACTGGGGGTGTGGTACAGCT

GACGTCCCAGTGGCTGACTAATATATTCGGGACCGTATATGAGAAG

CTCAAGCCAGTGCTGGACTGGCTGGAAGAGAAATTCAAGGAAGGT

GTGGAATTTTTGAGGGACGGCTGGGAGATTGTGAAGTTTATTAGCA

CCTGTGCATGTGAGATTGTTGGCGGACAGATCGTGACATGTGCTAA

GGAAATAAAAGAGAGTGTACAAACGTTCTTCAAACTCGTGAATAAAT

TTTTAGCGCTATGTGCTGACTCCATCATAATTGGCGGAGCCAAGTTA

AAAGCGCTCAACTTGGGAGAGACATTTGTCACCCACTCCAAAGGTC

TGTATAGGAAGTGTGTAAAGTCCAGAGAGGAGACTGGACTACTAAT

GCCACTGAAGGCTCCCAAAGAGATCATCTTCTTAGAAGGAGAGACA

CTTCCGACTGAGGTCCTTACAGAAGAAGTGGTCCTCAAAACTGGTG

ATCTCCAGCCCCTAGAGCAGCCCACATCCGAGGCAGTCGAAGCCC

CACTGGTGGGCACACCTGTGTGCATCAATGGACTCATGTTACTCGA

AATCAAGGATACCGAGAAGTACTGCGCACTGGCCCCTAACATGATG

GTAACCAATAACACATTCACCCTGAAAGGTGGTTGATAA

8
Nsp3

ATGCTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCT
362

CCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGT

GGTTATACCTACTAAAAAGGCTAGTGGCACTACTGAAATGCTAGCG

AAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCC

GGGTCAGGGTTTAAATGGTTACACTGTAGAGGAGGCAAAGACAGTG

CTTAAAAAGTGTAAAAGTGCTTTTTACATTCTACCATCTATTATCTCT

AATGAGAAGCAAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGA

AATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTCTGT

GTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGG

TATTAAAATACAAGAGGGTGTGGTTGATTATGGTGCTAGATTTTACT

TTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAAC

GATCTAAATGAAACTCTTGTTACAATGCCACTTGGCTATGTAACACA

TGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAG

TGCCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTAT

AATGGTTATCTTACTTCTTCTTCTAAAACACCTGAAGAACATATT

GAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGG

ACAATCTACACAACTAGGTATAGAATTTCTTAAGAGAGGTGATAAAA

GTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAA

GTTATCACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGT

GAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAACCTCCACA

CGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCC

AACTTATTTGGATGGAGCTGATGTTACTAAAATAAAACCTCATAATTC

ACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTAC

GTGTTGAGGCTTTTGAGTACTACCACACAACTGATCCTAGTTTTCTG

GGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATACCC

ACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTT

ATCTTGCCACTGCATTGTTAACACTCCAACAAATAGAGTTGAAGTTT

AATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTG

AAGCTGCTAACTTTTGTGCACTTATCTTAGCCTACTGTAATAAGACA

GTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCA

ACATGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTA

AAACTTGTGGACAACAGCAGACAACCCTTAAGGGTGTAGAAGCTGT

TATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTC

AGATACCTTGTACGTGTGGTAAACAAGCTACAAAATATCTAGTACAA

CAGGAGTCACCTTGTTATGATGTCAGCACCACCTGCTCAGTATG

AACTTAAGCATGGTACATTTACTTGTGCTAGTGAGTACACTGGTAAT

TACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTTTGTAT

TGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCC

TATTACGGATGTCTACAAAGAAAACAGTTACACAACAACCATAA

AACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGAC

CCTAAGTTGGACAATTATTATAAGAAAGACAATTCTTATTTCACAGA

GCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCT

TCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATT

TAAACCAGTTAACTGGTTATAAGAAACCTGCTTCAAGAGAGCTTAAA

GTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTA

TAAACACTACACACCCTCTTTTAAGAAAGGAGCTAAATTGTTACATA

AACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTAT

AAACCAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGT

TGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAGGACGCGCAG

GGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAG

AAGTAGTGGAAAATCCTACCATACAGAAAGACGTTCTTGAGTGTAAT

GTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAA

TAATAGTTTAAAAATTACAGAAGAGGTTGGCCACACAGATCTAATGG

CTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAATGAAT

TATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCT

GCTGTTAATAGTGTCCCTTGGGATACTATAGCTAATTATGCTAAGCC

TTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTG

TTTAAACCGTGTTTGTACTAATTATATGCCTTATTTCTTTACTTTATTG

CTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAAGCA

TCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAA

ATTTTGTCTAGAGGCTTCATTTAATTATTTGAAGTCACCTAATTTTTC

TAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTA

GGTTCTTTAATCTACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAAT

TTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTGAA

CTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTT

GTAGTGTTTGTCTTAGTGGTTTAGATTCTTTAGACACCTATCCTTCTT

TAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTG

CTTTTGGCTTAGTTGCAGAGTGGTTTTTGGCATATATTCTTTTCACTA

GGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTCA

GCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAA

TAATTAATCTTGTACAAATGGCCCCGATTTCAGCTATGGTTAGAATG

TACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGTGCAT

GTTGTAGACGGTTGTAATTCATCAACTTGTATGATGTGTTACAAACG

TAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTA

GAAGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAA

CTACACAATTGGAATTGTGTTAATTGTGATACATTCTGTGCTGGTAG

TACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTA

AAAGACCAATAAATCCTACTGACCAGTCTTCTTACATCGTTGATAGT

GTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGG

TCAAAAGACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGA

CAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTATTAATGTTA

TAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCA

GCGTCTGTTTACTACAGTCAGCTTATGTGTCAACCTATACTGTTACT

AGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCA

GTTAAAATGTTTGATGCTTACGTTAATACGTTTTCATCAACTTTTAAC

GTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGCTG

AACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTT

CAGCAGCTCGGCAAGGGTTTGTTGATTCAGATGTAGAAACTAAAGA

TGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTAC

TGGCGATAGTTGTAATAACTATATGCTCACCTATAACAAAGTTGAAA

ACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCG

TCATATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATAT

GGAACGTTAAAGATTTCATGTCATTGTCTGAACAACTACGAAAACAA

ATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATG

TGCAACTACTAGACAAGTTGTTAATGTTGTAACAACAAAGATAGCAC

TTAAGGGTGGTTGATGA

9
Nsp3

ATGCTCTTACTTGTTATTGACATCACCTTTCTAAAGAAAGATGCCCC
363

(codon
TTATATTGTGGGAGACGTTGTCCAGGAGGGAGTCCTTACGGCCGTG

optimized)
GTGATTCCAACAAAGAAGGCTAGTGGCACCACAGAAATGCTGGCCA

AGGCCTTGCGCAAGGTGCCGACAGACAACTACATCACTACATATCC

TGGACAAGGCCTGAACGGATATACAGTGGAAGAGGCAAAGACTGT

GCTAAAGAAGTGCAAGTCTGCCTTTTACATCCTTCCAAGCATTATTA

GCAACGAGAAGCAGGAAATACTCGGAACCGTAAGCTGGAACCTTA

GAGAGATGCTTGCGCATGCTGAGGAAACCCGAAAACTGATGCCTGT

GTGTGTTGAAACCAAAGCCATCGTTTCTACCATCCAGCGAAAGTATA

AGGGTATCAAGATCCAAGAGGGAGTGGTAGACTATGGCGCTAGATT

CTACTTCTATACCTCTAAAACAACTGTCGCCTCTCTTATCAATACTCT

GAATGACCTGAATGAGACTCTTGTGACCATGCCCCTGGGATACGTA

ACCCATGGGCTTAACTTGGAAGAAGCCGCTCGCTACATGCGTAGCC

TGAAGGTCCCTGCTACTGTTAGTGTCTCTTCCCCCGATGCCGTCAC

AGCTTACAACGGGTATCTGACGTCAAGCTCCAAAACTCCTGAAGAG

CACTTCATCGAGACCATTAGTCTAGCAGGATCCTATAAAGACTGGA

GTTACTCAGGCCAGAGCACCCAGCTGGGGATAGAGTTCTTGAAAC

GTGGAGACAAGTCCGTCTACTACACATCAAATCCCACGACGTTCCA

CCTGGATGGGGAAGTGATAACCTTTGATAACTTAAAAACCCTGCTG

AGCCTGAGGGAAGTCCGGACTATCAAGGTTTTTACTACAGTGGATA

ATATTAACCTTCACACACAGGTGGTGGACATGTCCATGACCTATGG

GCAGCAGTTCGGTCCTACCTACCTGGACGGAGCTGACGTTACCAAA

ATCAAACCCCACAACTCTCATGAGGGAAAGACGTTCTACGTGCTGC

CCAATGATGACACCTTGCGAGTGGAGGCATTTGAGTATTACCACAC

TACCGATCCGTCTTTCTTAGGCCGCTATATGTCCGCTTTAAATCATA

CAAAGAAGTGGAAGTACCCTCAGGTAAACGGCCTGACATCTATTAA

ATGGGCCGACAACAACTGCTATCTGGCAACTGCTTTGCTCACACTT

CAGCAGATTGAGTTGAAATTTAACCCTCCCGCACTGCAGGACGCTT

ATTATCGGGCAAGGGCCGGTGAGGCGGCTAACTTCTGTGCTCTCAT

CCTTGCTTACTGTAACAAAACAGTGGGCGAGCTAGGAGATGTCAGG

GAGACAATGTCTTACTTGTTTCAGCACGCCAACTTAGACTCCTGCAA

AAGAGTGCTCAATGTAGTCTGCAAAACCTGCGGTCAGCAGCAGACT

ACCTTGAAGGGGGTCGAAGCAGTGATGTACATGGGTACACTATCCT

ATGAGCAATTTAAAAAGGGTGTTCAGATCCCCTGCACATGTGGCAA

GCAGGCAACAAAATACCTCGTGCAGCAAGAATCCCCATTTGTTATG

ATGAGCGCACCTCCAGCCCAGTACGAACTGAAACATGGAACATTTA

CCTGCGCTTCGGAGTACACTGGCAATTACCAGTGTGGGCATTACAA

GCACATCACGTCCAAAGAAACACTCTACTGCATAGATGGAGCCCTG

TTGACCAAGTCCAGCGAATATAAAGGCCCTATCACAGATGTTTTCTA

CAAGGAGAATTCATATACAACCACCATCAAGCCCGTGACATACAAG

TTAGACGGCGTGGTATGTACAGAAATTGACCCCAAGCTGGACAATT

ACTACAAAAAAGACAATAGCTATTTTACTGAACAACCAATCGATCTT

GTCCCTAATCAGCCCTACCCCAATGCGTCATTTGATAACTTTAAGTT

TGTGTGTGATAATATTAAATTTGCAGATGATCTAAACCAGTTGACGG

GATACAAGAAACCCGCCTCGCGCGAACTGAAAGTGACTTTTTTTCC

AGATCTGAATGGGGATGTCGTGGCCATAGATTATAAGCATTATACTC

CAAGTTTCAAGAAAGGCGCTAAGTTATTACATAAGCCTATTGTCTGG

CATGTCAACAATGCTACAAATAAAGCCACTTATAAGCCAAACACATG

GTGTATTAGGTGCCTGTGGAGCACAAAACCAGTGGAGACTAGCAAT

TCCTTTGACGTCCTGAAGAGTGAAGATGCACAAGGCATGGATAACT

TGGCCTGTGAGGACCTGAAACCAGTCTCAGAGGAAGTGGTGGAAA

ATCCAACCATCCAGAAAGACGTACTGGAGTGTAACGTGAAAACCAC

TGAGGTTGTGGGGGACATTATCTTAAAACCTGCTAACAACAGCCTG

AAGATTACCGAGGAGGTAGGACACACTGATTTAATGGCAGCTTACG

TAGATAACTCCAGTCTGACCATCAAGAAGCCTAACGAGCTGAGTCG

GGTGCTAGGCCTCAAAACTCTGGCCACCCACGGCCTAGCGGCCGT

TAATTCTGTACCGTGGGATACAATCGCTAATTATGCCAAGCCCTTCC

TCAACAAGGTTGTCAGCACGACCACCAACATCGTGACCCGATGCTT

GAACCGTGTGTGCACGAACTATATGCCCTATTTCTTTACCCTTCTAC

TCCAGCTGTGTACTTTCACCCGGTCTACAAACAGTCGGATCAAGGC

CAGCATGCCAACCACAATTGCCAAGAACACCGTTAAATCCGTTGGG

AAGTTCTGCCTTGAGGCTAGCTTCAACTACCTGAAATCACCAAACTT

CTCGAAGCTGATTAACATTATAATATGGTTCCTGCTCCTGTCCGTGT

GTCTTGGAAGCCTTATCTACTCCACAGCTGCCCTGGGCGTCCTCAT

GTCTAATCTGGGTATGCCTAGTTACTGCACAGGCTACCGGGAAGGT

TATTTGAATAGCACTAACGTTACAATCGCCACATACTGCACAGGGA

GCATTCCTTGCTCCGTTTGTTTGTCAGGTTTGGATTCTCTCGATACT

TATCCCTCTCTGGAAACTATTCAGATCACGATTTCTAGTTTCAAATG

GGACCTCACGGCATTTGGGCTCGTGGCCGAGTGGTTCCTGGCCTA

TATACTCTTCACTAGGTTCTTCTATGTCCTGGGGCTGGCTGCCATCA

TGCAGCTCTTTTTCAGTTATTTCGCAGTCCATTTTATCAGCAATAGCT

GGCTCATGTGGCTCATAATAAATTTAGTACAGATGGCACCAATCTCG

GCCATGGTGAGGATGTATATCTTTTTCGCATCCTTTTACTATGTGTG

GAAATCATATGTCCACGTGGTGGACGGATGCAATAGTAGCACGTGC

ATGATGTGCTACAAACGGAATAGAGCGACCCGCGTGGAATGTACCA

CCATTGTGAATGGGGTTCGCAGATCATTCTACGTATATGCCAACGG

CGGCAAGGGCTTCTGCAAGCTCCATAACTGGAACTGTGTCAATTGT

GACACATTGTGCAGGATCAACTTTCATCAGTGACGAGGTCGCGC

GGGACCTCAGTCTGCAATTCAAGAGACCGATCAACCCTACCGACCA

GTCATCTTACATCGTGGATTCAGTTACCGTCAAGAATGGATCCATTC

ACTTATACTTCGATAAAGCAGGCCAAAAGACTTACGAGAGGCACTC

TCTCAGCCACTTTGTGAACCTGGACAATCTGAGGGCAAATAACACC

AAGGGGTCTCTTCCCATTAATGTGATAGTGTTTGACGGGAAGAGCA

AGTGTGAGGAGAGCAGCGCCAAATCTGCCTCAGTGTACTACAGCC

AGCTCATGTGTCAACCAATACTCCTTCTAGACCAAGCCCTGGTCTC

AGACGTTGGGGACAGTGCTGAAGTGGCTGTGAAGATGTTCGATGC

TTATGTGAACACATTTTCTTCCACTTTCAACGTCCCTATGGAGAAAC

TAAAGACGCTGGTGGCAACGGCCGAGGCCGAGCTGGCCAAGAAC

GTTTCATTGGACAATGTGCTCTCGACATTCATCTCCGCTGCAAGACA

GGGCTTTGTGGACTCAGATGTAGAAACCAAGGATGTGGTCGAGTG

CTTGAAGTTGTCGCACCAGAGCGACATCGAAGTCACAGGTGACAG

CTGCAATAATTACATGCTGACCTATAACAAGGTAGAGAACATGACAC

CACGCGATTTGGGAGCCTGCATAGACTGTAGTGCTAGGCACATTAA

TGCACAGGTCGCAAAATCTCATAACATCGCGCTAATCTGGAATGTC

AAAGATTTCATGAGCCTGTCTGAGCAATTGAGAAAGCAAATCAGGA

GTGCAGCTAAAAAAAATAACCTCCCGTTCAAGCTAACATGTGCCAC

AACTAGACAGGTGGTGAACGTGGTAACTACTAAAATTGCGCTGAAG

GGTGGTTGATAA

10
Nsp4
ATGAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACTT
364

GTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCAT

GTCATGTCTAAACATACTGACTTTTCAAGTGAAATCATAGGATACAA

GGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACT

TGTTTTGCTAACAAACATGCTGATTTTGACACATGGTTTAGCCAGCG

TGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAG

TCATAACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCAC

GATATTACGCACAACTAATGGTGACTTTTTGCATTTCTTACCTAGAG

TTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATA

GAGTACACTGACTTTGCAACATCAGCTTGTGTTTTGGCTGCTGAATG

TACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGA

TACCAATGTACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTG

ACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTCCTAAC

ACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGA

GTACTGTAGGCACGGCACTTGTGAAAGATCAGAAGCTGGTGTTTGT

GTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATC

TTTACCAGGAGTTTTCTGTGGTGTAGATGCTGTAAATTTATTTACTAA

TATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAG

CATCTATAGTAGCTGGTGGTATTGTGGCTATCGTAGTAACATGCCTT

GCCTACTATTTTATGAGGTTTAGAAGAGCTTTTGGTGAATACAGTCA

TGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCATTGTACT

CTGTTTAACACCAGTTTACTCATTCTTACCTGGTGTTTATTCTGTTAT

TTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCA

CATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGAT

AACAATTGCTTATATCATTTGTATTTCCACAAAGCATTTCTATTGGTT

CTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTT

TAGTACTTTTGAAGAAGCTGCGCTGTGCACCTTTTTGTTAAATAAAG

AAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTTTTACGCAA

TATAATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGA

GCAATGGATACAACTAGCTACAGAGAAGCTGCTTGTTGTCATCTCG

CAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTAC

CAACCACCACAAATCTCTATCACCTCAGCTGTTTTGCAGTGATGA

11
Nsp4
ATGAAAATCGTCAACAACTGGCTGAAACAGCTGATCAAAGTCACCTT
365

(codon
AGTGTTCCTGTTCGTGGCAGCCATTTTCTATCTGATCACCCCTGTGC

optimized)
ATGTCATGTCAAAGCATACAGATTTTTCTAGTGAAATTATCGGTTATA

AAGCTATCGACGGGGGGGTGACTAGGGACATAGCGTCGACTGATA

CTTGCTTTGCAAACAAGCACGCAGATTTGATACCTGGTTTTCTCAA

CGCGGAGGCTCCTACACAAATGACAAGGCCTGCCCACTCATCGCC

GCTGTTATTACCAGAGAGGTAGGCTTCGTTGTTCCTGGGCTCCCAG

GAACCATTCTGCGGACGACTAACGGAGATTTTCTCCACTTTCTGCC

TAGAGTGTTTTCAGCTGTAGGAAACATATGTTACACACCCTCGAAGC

TGATTGAGTACACCGACTTCGCAACTTCAGCATGTGTTCTCGCTGC

AGAGTGCACCATCTTCAAGGACGCCTCCGGGAAGCCGGTCCCTTA

CTGCTATGACACCAATGTGTTGGAAGGTTCCGTGGCCTATGAGTCA

CTCCGTCCTGACACTCGGTATGTGTTGATGGATGGCAGCATCATTC

AGTTTCCCAACACATATCTCGAGGGGAGTGTGAGGGTCGTGACCAC

ATTTGACAGTGAATATTGCAGGCACGGAACCTGTGAACGATCCGAG

GCCGGAGTATGCGTAAGCACATCAGGGAGGTGGGTACTCAATAAT

GATTATTACCGTTCTTTGCCAGGGGTGTTCTGTGGTGTGGATGCGG

TCAATCTCTTTACAAACATGTTTACTCCGTTAATTCAGCCAATAGGC

GCTCTGGACATATCTGCTTCTATAGTAGCTGGCGGCATTGTTGCCA

TTGTTGTGACATGCCTCGCATACTACTTCATGAGGTTCCGGCGAGC

CTTCGGTGAGTATTCTCATGTGGTGGCGTTCAATACACTGCTGTTC

CTGATGAGCTTCATTGTGCTGTGTCTGACACCAGTGTATTCCTTTTT

GCCTGGTGTCTATTCTGTGATATACCTGTATTTGACCTTCTACCTTA

CCAATGATGTGTCGTTCCTAGCGCACATCCAGTGGATGGTGATGTT

CACTCCCTTAGTCCCCTTCTGGATCACAATCGCCTACATCATCTGCA

TCAGCACCAAACATTTTTACTGGTTCTTCTCAAACTACCTGAAGCGC

CGCGTGGTCTTTAATGGAGTCTCCTTCAGCACTTTTGAAGAGGCTG

CCCTATGTACGTTTCTTCTCAACAAGGAGATGTACCTGAAACTAAGA

TCTGATGTTCTGCTCCCATTCACGCAGTACAACAGATATCTTGCACT

TTACAACAAATACAAGTACTTTAGTGGCGCCATGGACACAACGTCCT

ACCGGGAAGCTGCTTGTTGTCACCTGGCCAAGGCCCTGAATGACTT

TAGCAACAGCGGCAGTGACGTATTGTATCAGCCCCCCCAGATCTCC

ATTACTAGCGCAGTGTTACAATGATAA

12
Nsp5,
ATGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGG
366

Nsp6,
GTTGTATGGTACAAGTAACTTGTGGTACAACTACACTTAACGGTCTT

Nsp7,
TGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCT

Nsp8,
CTGAAGATATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGT

Nsp9,
CTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACTCAGGGTT

Nsp10
ATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATAC

AGCCAATCCTAAGACACCTAAGTATAAGTTTGTTCGCATTCAACCAG

GACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGT

GTTTACCAATGTGCTATGAGACACAATTTCACTATTAAGGGTTCATT

CCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGACT

GTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTT

CATGCTGGCACAGACTTAGAAGGTAACTTTTATGGACCTTTTGTTGA

CAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTT

AATGTTTTAGCTTGGTTGTACGCTGCTGTTATAAATGGAGACAGGTG

GTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGG

CTATGAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATA

CTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTTTAGATATGTG

TGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCA

TATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTG

TTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACA

ATCAAGGGTACACACCACTGGTTGTTACTCACAATTTTGACTTCACT

TTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTTTTTTGTA

TGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGC

TTTTGCAATGATGTTTGTCAAACATAAGCATGCATTTCTCTGTTTGTT

TTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATAT

GCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATATGGTT

GATACTAGTTTGAAGCTAAAAGACTGTGTTATGTATGCATCAGCTGT

AGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTG

CTAGGAGAGTGTGGACACTTATGAATGTCTTGACACTCGTTTATAAA

GTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCT

TATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCAT

GTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGTATTGCCCTA

TTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATT

GTTTCTTAGGCTATTTTTGTACTTGTTACTTTGGCCTCTTTTGTTTAC

TCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTT

CTACACAGGAGTTTAGATATATGAATTCACAGGGACTACTCCCACC

CAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGTTGGGTG

TTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATG

TCAGATGTAAAGTGCACATCAGTAGTCTTACTCTCAGTTTTGCAACA

ACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGT

TACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAA

AAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTGT

AGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACC

TTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGC

TTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGT

GATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGAATGTGGC

TAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAA

AAGATGGCTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGAT

CTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAATGCT

TTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTA

TCAACAATGCAAGAGATGGTTGTGTTCCCTTGAACATAATACCTCTT

ACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATA

TAAAAATACGTGTGATGGTACAACATTTACTTATGCATCAGCATTGT

GGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTT

AGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTAT

TGTAACAGCTTTAAGGGCCAATTCTGCTGTCAAATTACAGAATAATG

AGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTAC

TACACAAACTGCTTGCACTGATGACAATGCGTTAGCTTACTACAACA

CAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACA

GGATTTGAAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACT

ATTTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGACACACC

TAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAA

CCTAAATAGAGGTATGGTACTTGGTAGTTTAGCTGCCACAGTACGT

CTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATT

ATCTTTCTGTGCTTTTGCTGTAGATGCTGCTAAAGCTTACAAAGATT

ATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTTG

TGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAG

CCAATATGGATCAAGAATCCTTTGGTGGTGCATCGTGTTGTCTGTAC

TGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTT

AAAAGGTAAGTATGTACAAATACCTACAACTTGTGCTAATGACCCTG

TGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTG

GAAAGGTTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTT

CAGTGATGA

13
Nsp5_,
ATGAGTGGCTTCCGGAAGATGGCTTTTCCATCAGGCAAAGTGGAAG
367

Nsp6,
GCTGCATGGTCCAGGTGACCTGTGGAACCACTACATTGAACGGCCT

Nsp7,
CTGGCTGGATGACGTCGTATACTGTCCTAGACACGTGATCTGCACG

Nsp8,
TCAGAGGACATGCTCAATCCTAATTATGAAGATCTACTCATACGGAA

Nsp9,
GTCGAACCATAATTTCTTGGTGCAAGCAGGGAATGTGCAGCTGCGC

Nsp10
GTCATCGGCCACAGCATGCAAAACTGTGTACTGAAGTTGAAGGTGG

(codon
ACACTGCCAATCCTAAAACCCCCAAGTATAAGTTCGTGAGGATCCA

optimized)
GCCAGGACAAACTTTCAGTGTACTGGCGTGCTACAATGGCTCCCCT

TCTGGCGTCTATCAGTGTGCTATGCGCCATAATTTTACGATTAAGGG

TTCCTTCCTAAACGGATCATGCGGCAGTGTGGGCTTTAATATAGACT

ACGACTGCGTGAGTTTCTGCTATATGCATCACATGGAGCTACCAAC

AGGGGTCCATGCTGGAACTGACCTGGAGGGCAATTTCTATGGGCC

TTTTGTGGATCGTCAGACAGCACAAGCCGCCGGCACCGACACCAC

CATTACGGTAAATGTGTTAGCCTGGCTTTATGCTGCTGTAATCAATG

GAGACCGTTGGTTCCTGAACCGCTTTACGACAACCCTCAATGACTT

CAATCTAGTAGCCATGAAGTACAACTATGAGCCCTTAACACAGGAC

CACGTGGATATTCTGGGCCCGCTTAGCGCCCAGACCGGCATCGCG

GTTCTGGATATGTGTGCATCACTGAAAGAGCTTCTGCAGAACGGAA

TGAATGGAAGGACCATATTGGGTTCTGCGTTGCTTGAAGATGAGTT

CACCCCGTTTGATGTTGTGCGCCAGTGTAGCGGGGTCACCTTTCAG

TCTGCTGTGAAGCGGACTATAAAGGGAACACATCACTGGTTACTGC

TGACGATACTGACGTCTCTGCTGGTACTGGTACAGAGTACCCAGTG

GTCTTTGTTCTTTTTTCTCTACGAAAACGCGTTCCTGCCCTTTGCGA

TGGGCATTATTGCTATGTCCGCGTTCGCCATGATGTTTGTGAAGCA

CAAACATGCCTTCCTCTGCTTGTTTTTGCTCCCTTCTCTAGCAACTG

TGGCCTATTTCAACATGGTTTACATGCCAGCAAGCTGGGTGATGAG

GATTATGACCTGGCTTGACATGGTTGACACATCTCTGAAGCTTAAG

GACTGTGTCATGTACGCCAGCGCTGTAGTTCTGCTGATTCTCATGA

CAGCTCGCACCGTCTACGATGATGGGGCCAGACGAGTGTGGACAT

TAATGAACGTGTTAACTTTGGTTTACAAGGTTTACTATGGAAATGCC

CTTGATCAGGCAATCAGCATGTGGGCATTAATCATCTCAGTCACTA

GCAACTACAGTGGGGTCGTTACCACAGTGATGTTCCTAGCTCGAGG

AATCGTGTTCATGTGCGTGGAGTACTGTCCCATCTTCTTCATCACTG

GTAACACATTGCAGTGCATCATGCTGGTATATTGCTTCCTGGGCTA

CTTCTGTACATGTTACTTTGGACTATTTTGCCTGCTGAACCGCTACT

TCCGGCTGACGTTGGGAGTGTACGACTACCTCGTCTCGACCCAGG

AGTTCAGGTACATGAATAGTCAGGGCCTCCTGCCGCCCAAGAACTC

CATTGACGCTTTCAAGCTGAACATCAAACTTCTTGGGGTGGGTGGC

AAGCCCTGTATTAAGGTGGCCACTGTGCAATCCAAGATGTCTGACG

TCAAGTGTACCAGCGTCGTGTTACTGTCAGTGCTACAGCAGTTACG

AGTGGAAAGTTCTAGCAAGCTTTGGGCTCAATGCGTCCAGCTGCAC

AATGACATTCTTCTAGCCAAAGACACCACTGAAGCTTTTGAGAAAAT

GGTCTCGTTGCTCTCCGTGCTGTTGTCCATGCAGGGGGCTGTGGA

CATCAACAAGCTATGTGAAGAGATGCTGGACAACAGAGCTACCCTC

CAAGCCATTGCCTCAGAATTTTCCTCCCTACCCTCTTACGCTGCATT

TGCTACAGCGCAGGAAGCCTACGAGCAGGCAGTGGCAAACGGTGA

TAGTGAAGTAGTTCTGAAAAAGCTGAAGAAGTCCCTGAATGTCGCA

AAATCCGAGTTTGACAGAGATGCCGCTATGCAGCGGAAACTGGAGA

AGATGGCTGACCAGGCAATGACTCAGATGTATAAGCAGGCAAGGTC

AGAGGACAAAAGGGCTAAGGTCACATCGGCTATGCAGACGATGCT

GTTTACCATGCTCAGAAAACTCGACAATGACGCGCTCAACAATATTA

TCAATAACGCCCGCGATGGCTGTGTTCCACTAAACATCATCCCCCT

TACCACTGCTGCTAAACTCATGGTTGTGATTCCAGATTACAACACCT

ATAAAAATACTTGCGATGGTACCACATTTACATATGCCAGTGCCCTC

TGGGAGATTCAGCAAGTGGTGGATGCCGACAGCAAAATTGTGCAG

CTTTCTGAAATCTCTATGGATAACAGCCCCAACCTAGCTTGGCCACT

GATCGTGACTGCACTCCGGGCCAATTCCGCAGTCAAGTTGCAGAAT

AATGAGCTGTCCCCTGTTGCCCTTCGACAGATGAGCTGCGCAGCA

GGAACAACACAGACAGCCTGTACAGATGATAATGCTTTGGCCTATT

ACAACACAACCAAGGGTGGAAGATTTGTCCTGGCCCTGCTTTCAGA

CCTGCAAGACCTTAAGTGGGCCAGATTCCCTAAGTCTGACGGGACT

GGTACTATCTATACCGAATTGGAGCCTCCATGCAGGTTCGTCACTG

ATACTCCCAAAGGGCCGAAGGTGAAATATTTATATTTTATCAAGGGA

TTAAATAACCTGAACCGGGGCATGGTGCTGGGGAGCTTGGCAGCC

ACGGTAAGGCTACAAGCTGGAAACGCCACAGAGGTTCCAGCGAAC

AGTACAGTTCTGAGCTTCTGTGCGTTCGCCGTAGATGCAGCAAAAG

CATACAAAGATTACCTGGCCAGCGGAGGACAGCCTATCACAAACTG

CGTCAAAATGCTGTGCACCCACACGGGAACTGGACAGGCCATAACT

GTTACCCCAGAGGCTAACATGGACCAGGAGTCTTTTGGGGGTGCTT

CCTGCTGTCTGTACTGCCGTTGCCACATTGATCATCCAAACCCTAAA

GGGTTTTGTGATCTTAAGGGTAAATATGTGCAGATACCTACAACATG

CGCCAACGATCCCGTGGGGTTCACTCTGAAAAATACCGTGTGTACC

GTCTGTGGCATGTGGAAAGGCTATGGTTGCAGCTGCGATCAACTCA

GAGAACCCATGCTTCAATGATAA

14
Nsp12

ATGTCAGCTTGCACAATCGTTTTTAAACGGGTTTGCGGTGTAAGTG
368

CAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGT

ATACAGGGCTTTTGACATCTACAATGATAAAGTAGUGGTTTTGCTA

AATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGAT

GACAATTTAATTGATTCTTACTTTGTAGTTAAGAGACACACTTTCTCT

AACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTGTCCA

GCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACAT

GGTACCACATATATCACGTCAACGTCTTACTAAATACACAATGGCAG

ACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACA

TTAAAAGAAATACTTGTCACATACAATTGTTGTGATGATGATTATTTC

AATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACG

CGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAA

ACAGTACAATTCTGTGATGCCATGCGAAATGCTGGTATTGTTGGTGT

ACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCG

GTGATTTCATACAAACCACGCCAGGTAGTGGAGTTCCTGTTGTAGA

TTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTT

AACTGCAGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTA

AGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGTTAAAACTC

TTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTG

TGTTAACTGTTTGGATGACAGATGCATTCTGCATTGTGCAAACTTTA

ATGTTTTATTCTCTACAGTGTTCCCACTTACAAGMTTGGACCACTA

GTGAGAAAAATATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGG

ATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAAACT

TACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCT

GACCCTGCTATGCACGCTGCTTCTGGTAATCTATTACTAGATAAACG

CACTACGTGCTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTC

AAACTGTCAAACCCGGTAATTAACAAAGACTTCTATGACTTTGCT

GTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACA

CTTCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACT

ACTATCGTTATAATCTACCAACAATGTGTGATATCAGACAACTACTAT

TTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGC

TGTATTAATGCTAACCAAGTCATCGTCAACAACCTAGACAAATCAGC

TGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTC

AATGAGTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTA

ATGTCATCCCTACTATAACTCAAATGAATCTTAAGTATGCCATTAGT

GCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTA

CTATGACCAATAGACAGTTTCATCAAAAATTATTGAAATCAATAGCC

GCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATG

GTGGTTGGCACAACATGTTAAAAACTGTTTATAGTGATGTAGAAAAC

CCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATGC

CTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACAT

ACAACGTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGA

GTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTA

TATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTT

ATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTT

AATGCACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGT

CCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAGAAATAGAG

ATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAA

CATTTCTCAATGATGATACTTTCTGACGATGCTGTTGTGTGTTTCAAT

AGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAA

GTCAGTTCTTTATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATG

TTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTGCTCTC

AACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCT

TACCCAGATCCATCAAGAATCCTAGGGGCCGGCTGTTTTGTAGATG

ATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCT

TTAGCTATAGATGCTTACCCACTTACTAAACATCCTAATCAGGAGTA

TGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGA

TGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTA

ATGATAACACTTCAAGGTATTGGGAACCTGAGTTTTATGAGGCTATG

TACACACCGCATACAGTCTTACAGTGATGA

15
Nsp
ATGTCTGCCTGCACAATTGTGTTCAAGCGGGTGTGTGGAGTGTCTG
369

(codon
CAGCGCGATTAACTCCCTGTGGAACCGGCACCTCAACAGACGTAGT

optimized)
GTACCGTGCCTTCGATATTTACAATGATAAGGTGGCCGGGTTCGCG

AAATTCCTAAAGACGAACTGTTGCAGGTTCCAGGAGAAGGATGAAG

ATGACAATCTCATAGATTCTTATTTCGTGGTTAAACGGCATACATTTA

GTAATTACCAACATGAAGAAACAATCTACAACCTCCTCAAAGACTGT

CCTGCTGTGGCAAAACATGACTTCTTCAAGTTCCGGATTGACGGCG

ACATGGTTCCACACATCTCTCGGCAGAGATTAACAAAGTACACCAT

GGCTGACCTTGTATATGCACTGCGACACTTTGATGAAGGAAATTGC

GATACTCTGAAAGAGATTCTTGTTACCTACAACTGCTGTGATGACGA

CTACTTCAACAAGAAAGACTGGTACGACTTTGTAGAAAACCCAGATA

TCCTCAGAGTTTACGCCAACTTAGGAGAGCGCGTAAGACAAGCCCT

GTTAAAAACAGTTCAGTTCTGTGATGCCATGAGGAATGCAGGAATC

GTGGGAGTCTTGACCCTGGACAACCAGGACTTGAATGGGAACTGG

TATGACTTCGGGGATTTCATCCAGACTACACCCGGCAGCGGAGTGC

CAGTGGTGGATAGCTATTACTCCTTGCTGATGCCCATCCTTACGTT

GACAAGAGCCCTGACAGCAGAGAGCCATGTGGACACTGACCTTAC

CAAACCCTACATCAAGTGGGACTTACTCAAGTATGACTTCACAGAA

GAGCGGCTAAAGTTGTTCGATCGCTACTTTAAATATTGGGATCAGA

CCTATCATCCCAACTGTGTGAATTGTCTGGATGACAGGTGCATATTG

CACTGTGCAAACTTCAACGTTCTTTTTTCCACGGTTTTCCCCCTGAC

CAGCTTTGGGCCTCTGGTGAGAAAGATCTTTGTCGACGGGGTACCA

TTTGTTGTCAGTACCGGCTACCATTTCAGGGAACTCGGTGTCGTGC

ACAATCAGGATGTAAACCTGCACAGCAGCCGGCTAAGCTTTAAAGA

ACTGCTTGTTTATGCTGCTGATCCAGCCATGCACGCTGCCAGCGGA

AATTTACTCCTGGACAAGCGTACTACCTGTTTCAGTGTGGCAGCCC

TCACCAACAACGTAGCCTTCCAAACCGTGAAACCAGGCAACTTTAA

CAAAGATTTCTACGACTTTGCCGTTTCAAAGGGGTTTTTTAAGGAGG

GGAGTTCCGTGGAACTGAAGCACTTTGCACAAGACGGTAAT

GCTGCTATAAGCGACTATGATTATTACCGGTATAATCTGCCAACGAT

GTGTGATATTAGGCAACTGCTCTTCGTGGTAGAGGTCGTGGACAAA

TACTTTGACTGCTACGATGGCGGCTGCATCAATGCTAATCAGGTGA

TTGTCAACAATCTCGACAAGAGTGCGGGCTTTCCTTTCAATAAATGG

GGCAAGGCCCGCCTCTACTATGACAGCATGAGCTACGAGGACCAA

GATGCTCTGTTTGCTTATACAAAGAGAAATGTGATCCCTACCATCAC

ACAGATGAACCTTAAATATGCCATCTCCGCGAAGAACCGTGCCCGA

ACAGTTGCTGGTGTCTCCATTTGTTCTACCATGACAAACCGCCAGTT

CCACCAGAAGCTGTTGAAGAGCATAGCAGCTACTAGGGGCGCCAC

CGTCGTAATCGGGACATCCAAGTTTTATGGAGGGTGGCACAACATG

CTTAAAACGGTTTACTCAGATGTGGAGAATCCCCACCTAATGGGCT

GGGACTACCCCAAGTGTGACCGAGCAATGCCGAACATGCTCCGCA

TTATGGCAAGTCTGGTCCTCGCCAGAAAACACACGACTTGCTGCTC

ATTGTCACACAGGTTTTATCGTCTGGCCAATGAATGTGCCCAGGTC

CTGTCAGAGATGGTCATGTGCGGAGGATCCTTATATGTGAAGCCAG

GTGGCACTAGTTCTGGTGATGCCACAACTGCGTACGCAAATAGTGT

GTTCAACATCTGCCAGGCTGTCACTGCGAATGTTAATGCGTTGCTG

TCCACTGATGGAAACAAAATTGCAGACAAATACGTGCGCAACCTGC

AGCATCGTTTATATGAGTGCCTATACAGAAACAGAGATGTGGACAC

CGATTTTGTGAACGAGTTCTATGCCTACCTGAGGAAGCATTTCTCAA

TGATGATCCTGTCGGATGATGCCGTTGTCTGCTTTAATTCTACTTAT

GCTTCACAGGGCCTGGTGGCTTCCATAAAAAACTTCAAGTCTGTGC

TGTATTACCAGAACAATGTGTTTATGTCTGAAGCTAAGTGCTGGACC

GAGACGGACCTCACCAAGGGTCCCCATGAGTTCTGCTCCCAGCAC

ACAATGCTTGTGAAGCAGGGGGACGATTATGTGTATCTCCCTTACC

CTGACCCTTCGAGGATACTGGGCGCCGGGTGTTTTGTAGACGACAT

TGTCAAAACTGACGGTACTCTAATGATCGAGCGGTTCGTGAGCCTG

GCTATTGATGCTTACCCACTGACCAAGCACCCGAATCAAGAGTACG

CAGATGTCTTTCACCTTTACCTTCAGTACATTCGGAAGCTTCATGAT

GAGCTGACCGGCCACATGCTGGACATGTATTCAGTGATGCTGACAA

ATGACAATACATCTAGGTACTGGGAGCCTGAATTTTATGAAGCTATG

TATACTCCTCATACCGTGTTGCAGTGATAA

16
Nsp13

ATGGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAAT
370

CACTGGGTTACATCCTACACAGGCACCTACACACCTCAGTGTTGAC

ACTAAATTCAAAACTGAAGGTTTATGTGTTGACGTACCTGGCATACC

TAAGGACATGACCTATAGAAGACTCATCTCTATGATGGGTTTTAAAA

TGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAA

GAAGCTATAAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGG

GGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTTTACAG

CTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTA

TGTTGATACACCTAATAATACAGATTTTTCCAGAGTTAGTGCTAAAC

CACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAA

GGACTTCCTTGGAATGTAGTGCGTATAAAGATTGTACAAATGTTAAG

TGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGG

CACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGA

CCTGAGCGCACCTGTTGTCTATGTGATAGACGTGCCACATGCTTTT

CCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTT

GATTACGTCTATAATCCGTTTATGATTGATGTTCAACAATGGGGTTT

TACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATG

GTAATGCACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTA

GCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACTATTGAAT

ATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAG

GTTCAACACATGGTTGTTAAAGCTGCATTATTAGCAGACAAATTCCC

AGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTC

AAGCTGATGTAGAATGGAAGTTCTATGATGCACAGCCTTGTAGTGA

CAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACATTC

TGACAAATTCACAGATGGTGTATGCCTATTTGGAATTGCAATGTCG

ATAGATATCCTGCTAATTCCATTGTTTGTAGATTTGACACTAGAGTG

CTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGT

AAATAAACATGCATTCCACACACCAGCTTTTGATAAAAGTGCTTG

TTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTG

AGTCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTA

AAGTCTGCTACGTGTATAACACGTTGCAATTTAGGTGGTGCTGTCT

GTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAAC

ATGATGATCTCAGCTGGCTTTAGCTTGTGGGTTTACAAACAATTTGA

TACTTATAACCTCTGGAACACTTTTACAAGACTTCAGTGATGA

17
Nsp
ATGGCTGAGAATGTCACAGGGCTGTTTAAGGACTGTTCCAAGGTGA
371

(codon
TAACAGGACTGCACCCAACACAGGCCCCTACGCACTTGTCTGTTGA

optimized)
CACAAAATTCAAAACAGAAGGACTCTGTGTGGATGTACCCGGCATC

CCTAAAGACATGACCTATCGCAGGCTCATCAGTATGATGGGCTTCA

AGATGAACTACCAGGTCAATGGCTACCCAAATATGTTCATCACCAG

GGAGGAAGCTATTAGGCATGTGAGGGCCTGGATTGGTTTTGACGT

GGAAGGCTGTCATGCAACAAGAGAAGCAGTGGGGACCAACTTACC

TCTGCAGCTGGGGTTCAGCACTGGGGTCAACCTGGTTGCTGTCCC

AACTGGATATGTTGATACCCCCAACAACACAGACTTCTCACGGGTC

TCTGCAAAGCCTCCACCTGGGGATCAGTTCAAGCACCTGATCCCAT

TAATGTATAAAGGTCTGCCCTGGAATGTGGTAAGGATCAAAATTGTT

CAGATGCTGAGTGACACTCTGAAGAACTTGAGTGACAGAGTTGTGT

TTGTCCTGTGGGCTCATGGCTTTGAGCTGACCAGCATGAAGTACTT

TGTGAAAATTGGACCAGAGAGAACGTGCTGCCTGTGTGACCGACG

GGCAACTTGTTTTTCTACCGCATCAGATACTTACGCCTGCTGGCAC

CATAGTATCGGCTTTGACTATGTCTACAACCCTTTCATGATTGATGT

CCAGCAATGGGGCTTCACAGGAAATTTGCAGTCCAACCACGACCTG

TACTGCCAGGTCCATGGGAATGCTCACGTGGCCTCCTGCGATGCTA

TCATGACACGATGTCTGGCTGTGCATGAGTGCTTTGTGAAGCGTGT

CGACTGGACCATCGAATACCCCATTATTGGCGACGAGCTAAAAATA

AATGCAGCGTGTAGAAAAGTGCAGCACATGGTGGTCAAGGCAGCA

CTCCTTGCTGACAAGTTTCCAGTATTACATGACATCGGCAACCCAAA

GGCCATTAAGTGTGTTCCTCAAGCGGATGTGGAGTGGAAGTTCTAT

GATGCCCAGCCGTGCTCTGATAAAGCCTACAAGATAGAAGAACTCT

TTTATTCTTACGCTACTCACAGCGACAAATTTACAGATGGAGTTTGC

CTCTTCTGGAACTGCAATGTCGATAGATATCCGGCCAACAGCATAG

TGTGCCGCTTTGATACGCGCGTGCTATCCAACTTGAACCTCCCGGG

TTGTGATGGCGGTTCGCTTTATGTAAATAAACATGCTTTCCACACAC

CTGCCTTCGACAAGTCCGCCTTTGTGAATCTGAAACAACTTCCCTTC

TTCTACTACAGCGACAGCCCCTGCGAGTCCCACGGGAAGCAGGTG

GTGAGTGATATTGACTATGTCCCCCTTAAGTCAGCGACTTGCATCA

CTCGCTGTAACCTTGGAGGAGCTGTTTGTCGGCACCACGCGAATGA

GTACCGACTCTACCTGGACGCCTATAACATGATGATCTCTGCCGGT

TTCTCACTATGGGTATATAAGCAGTTTGATACCTACAATTTGTGGAA

CACCTTCACCCGGCTCCAATGATAA

18
Or7a/7b

ATGAAAATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGA
372

GCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTTAA

AAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCAT

CCTCTAGCTGATAACAAATTTGCACTGACTTGCTTTAGCACTCAATT

TGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTG

CCAGATCAGTTTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAA

GAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGTTTATA

ACACTTTGCTTCACACTCAAAAGAAAGACAGAATGTGAACTTTCATT

AATTGACTTCTATTTGTGCTTTTTAGCCTTTCTGTTATTCCTTGTTTA

ATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAAT

GAAACTTGTCACGCCTAATGATGA

19
Orf7a/7b
ATGAAGATCATTTTGTTTCTAGCATTAATAACTCTAGCCACCTGTGA
373

(codon
GCTCTACCACTACCAGGAGTGTGTGAGGGGTACCACTGTACTGCTG

optimized)
AAGGAGCCCTGCAGCTCTGGAACATATGAAGGCAACAGCCCTTTCC

ACCCTTTGGCTGATAACAAGTTTGCTCTTACGTGCTTTTCTACTCAG

TTTGCATTTGCCTGCCCAGATGGGGTGAAGCATGTGTATCAGCTGC

GAGCGCGCAGTGTTTCCCCAAAACTCTTCATTCGGCAAGAAGAGGT

CCAAGAACTGTATAGTCCCATCTTTCTCATTGTGGCTGCCATCGTGT

TCATCACACTATGTTTCACCCTGAAAAGAAAAACAGAATGTGAACTT

TCATTGATTGACTTCTACCTGTGCTTCCTGGCCTTCCTCTTATTTCTT

GTTCTCATCATGCTGATCATCTTCTGGTTCTCCCTGGAGCTGCAGG

ACCACAATGAGACCTGCCATGCATGATAATGA

20
Membrane

ATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGC
374

TCCTTGAAGAATGGAACCTAGTAATAGGTTTCCTATTCCTTACATGG

ATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTAT

ATAATTAAGTTAATTTTCCTCTGGCTGTTATGGCCAGTAACTTTAACT

TGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGG

AATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGC

TACTTCATTGCTTCTTTCAGACTGTTTGCGCGTACGCGTTCCATGTG

GTCATTTAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATG

GCACTATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCGG

AGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTA

GGACGCTGTGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTA

CATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGCGTGTA

GCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCA

ACTATAAATTAAACACAGACCATTCCAGTAGCAGTGACAATATTGCT

TTGCTTGTACAGTAATGATGA

21
Membrane
ATGGCTGATTCAAATGGCACCATCACTGTGGAGGAACTGAAGAAGC
375

(codon
TCCTTGAGGAGTGGAACCTGGTGATTGGCTTCCTCTTCTTAACCTG

optimized)
GATCTGCCTGCTGCAGTTTGCTTATGCCAACAGAAATCGCTTCCTG

TACATCATCAAGCTCATCTTTCTCTGGCTACTTTGGCCTGTAACTTT

GACCTGCTTTGTGCTGGCAGCTGTGTACAGAATCAACTGGATTACT

GGAGGAATTGCGATAGCCATGGCCTGTTTAGTGGGGCTCATGTGG

CTGAGCTACTTCATCGCTTCCTTCCGGCTGTTTGCCCGAACGAGGA

GCATGTGGAGCTTCAACCCAGAAACAAATATCCTGCTTAATGTTCC

GCTGCATGGAACCATACTCACCAGGCCTCTATTGGAATCTGAGCTG

GTCATTGGAGCTGTCATACTCCGGGGCCATTTGCGAATTGCTGGCC

ACCACCTGGGTCGTTGTGACATCAAAGATCTGCCCAAGGAGATCAC

AGTGGCCACTTCTAGAACATTGTCTTACTATAAACTTGGGGCGAGT

CAGAGGGTTGCAGGTGATTCGGGCTTTGCAGCCTATAGCCGCTATA

GGATTGGGAACTACAAACTAAATACAGACCACTCATCCTCCAGTGA

CAACATTGCATTGCTTGTCCAATGATAATGA

22
Envelope

ATGTACTCATTCGTTTCGGAAGAGATAGGTACGTTAATAGTTAATAG
376

CGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGC

CATCCTTACTGCGCTTCGATTGTGTGCGTACTGCTGCAATATTGTTA

ACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAA

ATCTGAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAATGATGA

23
Envelope
ATGTACAGCTTTGTTAGTGAAGAGATTGGCACTCTAATAGTAAATTC
377

(codon
TGTGCTGCTCTTCCTTGCTTTTGTGGTGTTCCTGCTGGTCACCCTG

optimized)
GCCATCCTCACAGCATTAAGGCTCTGTGCCTACTGCTGCAACATCG

TCAATGTGTCTCTGGTGAAACCTTCCTTCTATGTGTATTCACGGGTG

AAGAACTTGAACAGCTCCAGAGTTCCAGACTTGCTTGTATGATAATG

A

24
Nucleo-

ATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACTCCGCATTA
378

capsid
CGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGTGG

GGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACT

GCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTTA

AATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCC

AGATGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGT

GGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGTATTTCT

ACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAA

CAAAGACGGCATCATATGGGTTGCAACTGAGGGAGCCTTGAATACA

CCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAA

TCGTGCTACAACTTCCTCAAGGAACAACATTGCCAAAAGGCTTCTAC

GCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCA

TCACGTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTA

AACGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGTGATGCTGC

TCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAA

ATGTCTGGTAAAGGCCAACAACAACAAGGCCAAACTGTCACTAAGA

AATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGC

CACTAAAGCATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCA

GAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAAG

GAACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAG

CGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCATGGAAGTCACA

CCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATG

ACAAAGATCCAAATTTCAAAGATCAAGTCATTTTGCTGAATAAGCAT

attgacgcatacaaaacattcccaccaacagagcctaaaaaggaca

AAAAGAAGAAGGCTGATGAAACTCAAGCCTTACCGCAGAGACAGAA

GAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGATT

TCTCCAAACAATTGCAACAATCCATGAGCCGTGCTGACTCAACTCA

GGCCTAATGATGA

25
Nucleo-
ATGTCAGACAATGGGCCCCAGAACCAGAGAAATGCCCTTCGCATCA
379

capsid
CGTTTGGTGGCCCTAGTGACAGCACCGGCAGCAATCAGAATGGAG

(codon
GTGCCCGCAGTAAACAAAGGAGACCACAGGGACTGCCAAACAACA

optimized)
CAGCATCCTGGTTTACTGCACTTACGCAGCATGGTAAGGAGGACCT

GAAGTTCCCAAGAGGACAGGGAGTCCCCATCAACACCAACAGCTC

ACCAGATGATCAGATCGGCTATTACCGGCGGGCTACTCGCCGCATA

CGAGGGGGCGATGGCAAGATGAAGGATCTAAGTCCGCGTTGGTAC

TTCTACTATCTGGGCACAGGGCCTGAAGCTGGCCTGCCTTATGGG

GCCAATAAGGATGGCATCATCTGGGTGGCCACTGAGGGGGCTTTG

AATACCCCAAAAGACCACATCGGGACTCGAAACCCTGCCAACAATG

CAGCCATTGTCCTTCAGCTCCCACAAGGGACAACATTACCCAAGGG

CTTCTATGCAGAGGGCTCCAGGGGAGGTTCTCAAGCCAGCAGCCG

GAGCAGCTCGCGGTCCCGGAACTCAAGCCGAAATTCCACTCCTGG

CAGCTCCAAGAGAACATCACCTGCGAGGATGGCTGGAAATGGAGG

CGATGCTGCTCTGGCCCTCCTTTTGCTGGACAGGCTCAACCAGCTG

GAGAGTAAAATGAGTGGAAAGGGGCAGCAGCAACAGGGCCAGACT

GTGACCAAGAAGTCTGCAGCAGAAGCGTCCAAGAAACCCAGGCAG

AAAAGGACAGCCACAAAAGCCTATAATGTGACACAAGCCTTTGGGA

GAAGAGGGCCAGAGCAGACCCAGGGAAACTTTGGAGACCAGGAGC

TGATTCGTCAAGGTACAGACTACAAGCACTGGCCACAGATTGCTCA

GTTTGCTCCCTCTGCATCAGCCTTCTTCGGCATGAGCAGGATAGGT

ATGGAGGTGACTCCGAGCGGAACCTGGCTGACCTACACCGGAGCC

ATCAAGCTGGATGACAAGGATCCCAACTTCAAGGACCAGGTAATTC

TCTTAAACAAGCATATTGATGCCTACAAAACCTTTCCTCCAACAGAA

CCCAAAAAGGACAAAAAAAAAAAAGCTGATGAAACTCAGGCCCTAC

CTCAGCGGCAAAAGAAACAACAGACGGTTACCCTACTCCCGGCTG

CAGATTTGGACGACTTCTCTAAGCAGCTGCAGCAGTCCATGTCCAG

AGCTGACTCTACCCAAGCATGATAATGA

26
Spike (2-

ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGC
380

proline
GTGAACCTGATCACCAGGACCCAGAGCTACACCAACAGCTTCACCA

substitute)
GGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCTGC

ACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTG

GTTCCACGCCATCAGCGGCACCAACGGCACCAAGAGGTTCGACAA

CCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGA

CAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGT

GGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTG

GACGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAG

TTCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGA

GCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCA

AGAACCTGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTTCAA

GATCTACAGCAAGCACACCCCCATCAACCTGGGCAGGGACCTGCC

CCAGGGCTTCAGCGCCCTGGAGCCCCTGGTGGACCTGCCCATCGG

CATCAACATCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAG

CTACCTGACCCCCGGCGACAGCAGCAGCGGCTGGACCGCCGGCG

CCGCCGCCTACTACGTGGGCTACCTGCAGCCCAGGACCTTCCTGC

TGAAGTACAACGAGAACGGCACCATCACCGACGCCGTGGACTGCG

CCCTGGACCCCCTGAGCGAGACCAAGTGCACCCTGAAGAGCTTCA

CCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCAGGGTGCAGC

CCACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACCTGTGCC

CCTTCGACGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGTACG

CCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGCG

TGCTGTACAACTTCGCCCCCTTCTTCGCCTTCAAGTGCTACGGCGT

GAGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGC

CGACAGCTTCGTGATCAGGGGCAACGAGGTGAGCCAGATCGCCCC

CGGCCAGACCGGCAACATCGCCGACTACAACTACAAGCTGCCCGA

CGACTTCACCGGCTGCGTGATCGCCTGGAACAGCAACAAGCTGGA

CAGCAAGGTGGGCGGCAACTACAACTACAGGTACAGGCTGTTCAG

GAAGAGCAACCTGAAGCCCTTCGAGAGGGACATCAGCACCGAGAT

CTACCAGGCCGGCAACAAGCCCTGCAACGGCGTGGCCGGCGTGA

ACTGCTACTTCCCCCTGAGGAGCTACGGCTTCAGGCCCACCTACG

GCGTGGGCCACCAGCCCTACAGGGTGGTGGTGCTGAGCTTCGAGC

TGCTGCACGCCCCCGCCACCGTGTGCGGCCCCAAGAAGAGCACCA

ACCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGCCTGAC

CGGCACCGGCGTGCTGACCGAGAGCAACAAGAAGTTCCTGCCCTT

CCAGCAGTTCGGCAGGGACATCGCCGACACCACCGACGCCGTGAG

GGACCCCCAGACCCTGGAGATCCTGGACATCACCCCCTGCAGCTT

CGGCGGCGTGAGCGTGATCACCCCCGGCACCAACACCAGCAACCA

GGTGGCCGTGCTGTACCAGGGCGTGAACTGCACCGAGGTGCCCGT

GGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTGTACAG

CACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGATCGG

CGCCGAGTACGTGAACAACAGCTACGAGTGCGACATCCCCATCGG

CGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAAGAGCCACCA

GCAGGCCCAGAGCGTGGCCAGCCAGAGCATCATCGCCTACACCAT

GAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCAT

CGCCATCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTG

CCCGTGAGCATGACCAAGACCAGCGTGGACTGCACCATGTACATCT

GCGGCGACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCA

GCTTCTGCACCCAGCTGAAGAGGGCCCTGACCGGCATCGCCGTGG

AGCAGGACAAGAACACCCAGGAGGTGTTCGCCCAGGTGAAGCAGA

TCTACAAGACCCCCCCCATCAAGTACTTCGGCGGCTTCAACTTCAG

CCAGATCCTGCCCGACCCCAGCAAGCCCAGCAAGAGGAGCTTCAT

CGAGGACCTGCTGTTCAACAAGGTGACCCTGGCCGACGCCGGCTT

CATCAAGCAGTACGGCGACTGCCTGGGCGACATCGCCGCCAGGGA

CCTGATCTGCGCCCAGAAGTTCAACGGCCTGACCGTGCTGCCCCC

CCTGCTGACCGACGAGATGATCGCCCAGTACACCAGCGCCCTGCT

GGCCGGCACCATCACCAGCGGCTGGACCTTCGGCGCCGGCGCCG

CCCTGCAGATCCCCTTCGCCATGCAGATGGCCTACAGGTTCAACG

GCATCGGCGTGACCCAGAACGTGCTGTACGAGAACCAGAAGCTGA

TCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGGACAGCC

TGAGCAGCACCGCCAGCGCCCTGGGCAAGCTGCAGGACGTGGTG

AACCACAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAGC

AGCAAGTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGC

AGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGAT

CACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCT

GATCAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCA

CCAAGATGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACT

TCTGCGGCAAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCC

CCCACGGCGTGGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGG

AGAAGAACTTCACCACCGCCCCCGCCATCTGCCACGACGGCAAGG

CCCACTTCCCCAGGGAGGGCGTGTTCGTGAGCAACGGCACCCACT

GGTTCGTGACCCAGAGGAACTTCTACGAGCCCCAGATCATCACCAC

CGACAACACCTTCGTGAGCGGCAACTGCGACGTGGTGATCGGCAT

CGTGAACAACACCGTGTACGACCCCCTGCAGCCCGAGCTGGACAG

CTTCAAGGAGGAGCTGGACAAGTACTTCAAGAACCACACCAGCCCC

GACGTGGACCTGGGCGACATCAGCGGCATCAACGCCAGCGTGGTG

AACATCCAGAAGGAGATCGACAGGCTGAACGAGGTGGCCAAGAAC

CTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGGCAAGTACGAG

CAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTTCATCGCCG

GCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTGCATGAC

CAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGGCAGCTG

CTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGCGT

GAAGCTGCACTACACCTGATGA

27
Spike (2-
ATGTTTGTCTTTTTGGTACTCCTCCCTCTTGTCTCGTCTCAGTGTGT
381

proline
CAACTTGATTACACGCACTCAAAGTTACACCAACTCCTTTACCCGAG

substitute)
GAGTCTACTATCCAGATAAAGTGTTCAGATCTAGCGTGTTGCATAGC

(codon
ACGCAGGATCTCTTTCTCCCGTTTTTCAGCAACGTGACCTGGTTCC

optimized)
ATGCAATATCTGGCACAAATGGGACCAAGCGGTTTGACAATCCTGT

GTTACCGTTCAATGATGGCGTGTACTTCGCCAGTACCGAAAAGTCC

AACATCATCAGAGGCTGGATCTTCGGCACAACCCTGGATTCTAAAA

CTCAGTCCCTTCTGATAGTCAACAACGCAACCAACGTGGTGATAAA

GGTGTGTGAATTCCAGTTTTGTAACGACCCCTTCCTTGACGTTTATT

ATCACAAGAATAACAAGAGTTGGATGGAGAGCGAATTTCGGGTATA

TTCCTCAGCAAACAACTGCACATTTGAATATGTGAGTCAGCCCTTCC

TCATGGACCTCGAAGGAAAGCAAGGCAACTTTAAGAACCTGAGGGA

GTTCGTGTTCAAGAACATCGACGGCTATTTAAGATCTACTCTAAGC

ACACCCCCATCAATCTTGGCAGAGACCTACCCCAGGGCTTTTCGGC

CTTGGAGCCTCTGGTGGATCTCCCCATCGGTATCAACATCACCCGA

TTCCAAACTCTCCTGGCACTGCACAGGTCATATCTAACACCCGGGG

ACAGCAGCAGTGGGTGGACAGCTGGCGCCGCGGCTTACTATGTTG

GCTACCTGCAGCCACGTACCTTCCTGCTGAAATACAATGAAAACGG

AACGATTACAGATGCGGTGGACTGTGCACTGGATCCATTGTCTGAA

ACTAAATGTACACTAAAATCCTTTACTGTTGAGAAGGGCATCTACCA

AACTTCTAATTTCAGGGTTCAGCCCACCGAATCCATCGTTCGCTTCC

CCAACATTACTAATCTGTGTCCATTTGATGAAGTGTTTAATGCCACC

CGCTTCGCTTCCGTGTATGCATGGAACAGGAAGAGAATCTCAAACT

GTGTGGCAGACTATTCTGTTCTGTACAATTTGCTCCTTTCTTTGCC

TTCAAGTGCTATGGAGTGTCACCTACAAAGCTCAATGACCTCTGCTT

CACAAATGTCTACGCCGATTCTTTTGTGATTCGTGGAAATGAAGTTT

CCCAGATCGCACCCGGCCAGACGGGAAACATAGCCGACTACAACT

ACAAGCTGCCGGATGACTTCACGGGCTGTGTCATTGCTTGGAATTC

AAATAAATTAGATAGTAAAGTGGGGGGGAACTATAATTACCGCTACC

GGCTTTTTCGCAAATCAAATTTGAAGCCATTTGAGCGTGATATTTCG

ACTGAAATCTATCAGGCTGGGAACAAACCCTGCAACGGGGTAGCA

GGAGTCAATTGCTACTTTCCTTTACGCAGCTACGGGTTCCGACCTA

CCTATGGTGTGGGCCACCAACCATACAGGGTGGTTGTCTTGTCCTT

CGAGCTTCTTCATGCCCCGGCAACGGTGTGCGGTCCTAAAAAGAGT

ACCAATCTAGTAAAGAATAAGTGTGTGAACTTTAACTTCAATGGTTT

GACCGGCACTGGCGTTTTGACTGAGTCCAACAAGAAGTTCTTACCC

TTTCAGCAGTTCGGGCGGGACATCGCCGACACTACAGATGCAGTG

CGCGATCCGCAGACACTGGAGATTTTGGACATTACCCCTTGCTCCT

TTGGTGGAGTATCTGTAATAACCCCAGGCACGAACACTAGTAATCA

AGTCGCCGTGCTGTATCAAGGTGTTAACTGTACCGAGGTGCCTGTG

GCTATTCATGCTGACCAGCTCACTCCCACATGGAGAGTCTACTCTA

CAGGTAGCAATGTATTTCAAACAAGGGCTGGGTGCCTGATAGGAGC

CGAGTACGTGAATAACTCTTATGAGTGTGACATCCCAATCGGAGCT

GGGATTTGTGCCTCTTACCAGACCCAGACGAAGTCACACCAGCAG

GCTCAATCAGTGGCGTCTCAGAGTATCATCGCCTACACTATGTCCC

TCGGAGCAGAAAATTCAGTTGCATACAGCAATAACTCCATCGCCATT

CCAACTAACTTCACTATATCGGTGACCACAGAGATCCTGCCAGTCA

GCATGACAAAAACATCAGTAGACTGCACCATGTATATATGCGGGGA

CAGCACAGAATGTTCTAACCTGCTGTTGCAATATGGGAGCTTCTGC

ACTCAGTTGAAGAGGGCTCTGACTGGAATCGCTGTGGAGCAGGAC

AAGAATACCCAGGAAGTGTTCGCCCAGGTGAAGCAGATATACAAAA

CCCCTCCAATCAAATACTTTGGCGGGTTTAACTTCAGCCAAATTCTG

CCCGATCCCTCCAAGCCTAGTAAAAGGTCCTTCATTGAAGATCTGC

TGTTCAACAAAGTCACACTGGCAGATGCTGGATTCATCAAGCAGTA

TGGTGACTGTCTGGGCGACATCGCAGCGCGGGATCTGATTTGTGC

ACAGAAGTTTAATGGGCTTACAGTCTTGCCTCCTCTGCTGACAGAC

GAGATGATTGCCCAGTACACGTCTGCCTTGCTCGCTGGTACCATAA

CCAGCGGATGGACCTTCGGAGCCGGCGCTGCCCTTCAGATCCCTT

TCGCTATGCAGATGGCCTATAGATTTAATGGCATCGGAGTGACTCA

GAACGTTCTCTACGAGAATCAGAAACTGATTGCCAACCAGTTTAACA

GTGCGATCGGAAAGATTCAAGACAGTCTATCCAGCACGGCTTCTGC

ACTGGGTAAACTGCAAGATGTGGTTAACCACAACGCCCAGGCCCTG

AACACACTCGTGAAACAGCTCTCTTCCAAGTTCGGTGCCATCAGCT

CAGTTCTTAACGATATACTGAGTAGACTAGACCCTCCAGAGGCCGA

AGTGCAGATCGACAGGCTTATCACAGGTCGGCTGCAGTCTCTACAA

ACCTATGTCACACAACAGTTAATCAGAGCAGCTGAGATCCGAGCTT

CCGCGAACCTCGCGGCAACAAAGATGTCTGAGTGCGTCCTGGGGC

AGAGCAAGCGGGTAGATTTCTGTGGGAAAGGATACCATCTGATGTC

ATTTCCCCAGAGCGCCCCACATGGTGTAGTTTTTCTGCATGTGACC

TACGTCCCTGCCCAAGAAAAAAATTTCACTACGGCTCCAGCTATCT

GCCACGATGGAAAAGCGCACTTCCCCCGAGAGGGCGTTTTTGTCA

GTAATGGTACCCACTGGTTTGTGACACAGAGAAATTTCTATGAGCC

ACAGATAATTACTACCGACAACACATTTGTCTCTGGGAATTGTGATG

TCGTAATTGGAATTGTGAACAATACAGTATATGATCCATTGCAGCCT

GAGCTAGACTCTTTCAAAGAAGAGCTGGATAAATATTTCAAGAACCA

TACCTCGCCTGACGTGGACTTGGGGGATATCTCCGGAATAAATGCA

TCCGTGGTGAATATCCAGAAGGAAATTGATAGACTGAATGAGGTGG

CCAAAAACCTCAATGAGAGTTTGATTGACCTTCAGGAGTTAGGCAA

GTATGAACAGTACATTAAGTGGCCATGGTACATCTGGCTGGGTTTC

ATTGCCGGCCTCATAGCAATTGTCATGGTGACCATCATGCTCTGCT

GCATGACTTCCTGCTGCAGCTGCCTTAAAGGCTGCTGTAGCTGTGG

GTCCTGTTGCAAATTTGATGAGGACGACTCAGAGCCGGTGCTGAAG

GGAGTTAAATTGCACTACACTTGATAA

28
Spike (6
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGC
382

proline
GTGAACCTGATCACCAGGACCCAGAGCTACACCAACAGCTTCACCA

substitute)
GGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCTGC

ACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTG

GTTCCACGCCATCAGCGGCACCAACGGCACCAAGAGGTTCGACAA

CCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGA

CAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGT

GGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTG

GACGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAG

TTCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGA

GCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCA

AGAACCTGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTTCAA

GATCTACAGCAAGCACACCCCCATCAACCTGGGCAGGGACCTGCC

CCAGGGCTTCAGCGCCCTGGAGCCCCTGGTGGACCTGCCCATCGG

CATCAACATCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAG

CTACCTGACCCCCGGCGACAGCAGCAGCGGCTGGACCGCCGGCG

CCGCCGCCTACTACGTGGGCTACCTGCAGCCCAGGACCTTCCTGC

TGAAGTACAACGAGAACGGCACCATCACCGACGCCGTGGACTGCG

CCCTGGACCCCCTGAGCGAGACCAAGTGCACCCTGAAGAGCTTCA

CCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCAGGGTGCAGC

CCACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACCTGTGCC

CCTTCGACGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGTACG

CCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGCG

TGCTGTACAACTTCGCCCCCTTCTTCGCCTTCAAGTGCTACGGCGT

GAGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGC

CGACAGCTTCGTGATCAGGGGCAACGAGGTGAGCCAGATCGCCCC

CGGCCAGACCGGCAACATCGCCGACTACAACTACAAGCTGCCCGA

CGACTTCACCGGCTGCGTGATCGCCTGGAACAGCAACAAGCTGGA

CAGCAAGGTGGGCGGCAACTACAACTACAGGTACAGGCTGTTCAG

GAAGAGCAACCTGAAGCCCTTCGAGAGGGACATCAGCACCGAGAT

CTACCAGGCCGGCAACAAGCCCTGCAACGGCGTGGCCGGCGTGA

ACTGCTACTTCCCCCTGAGGAGCTACGGCTTCAGGCCCACCTACG

GCGTGGGCCACCAGCCCTACAGGGTGGTGGTGCTGAGCTTCGAGC

TGCTGCACGCCCCCGCCACCGTGTGCGGCCCCAAGAAGAGCACCA

ACCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGCCTGAC

CGGCACCGGCGTGCTGACCGAGAGCAACAAGAAGTTCCTGCCCTT

CCAGCAGTTCGGCAGGGACATCGCCGACACCACCGACGCCGTGAG

GGACCCCCAGACCCTGGAGATCCTGGACATCACCCCCTGCAGCTT

CGGCGGCGTGAGCGTGATCACCCCCGGCACCAACACCAGCAACCA

GGTGGCCGTGCTGTACCAGGGCGTGAACTGCACCGAGGTGCCCGT

GGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTGTACAG

CACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGATCGG

CGCCGAGTACGTGAACAACAGCTACGAGTGCGACATCCCCATCGG

CGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAAGAGCCACCA

GCAGGCCCAGAGCGTGGCCAGCCAGAGCATCATCGCCTACACCAT

GAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCAT

CGCCATCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTG

CCCGTGAGCATGACCAAGACCAGCGTGGACTGCACCATGTACATCT

GCGGCGACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCA

GCTTCTGCACCCAGCTGAAGAGGGCCCTGACCGGCATCGCCGTGG

AGCAGGACAAGAACACCCAGGAGGTGTTCGCCCAGGTGAAGCAGA

TCTACAAGACCCCCCCCATCAAGTACTTCGGCGGCTTCAACTTCAG

CCAGATCCTGCCCGACCCCAGCAAGCCCAGCAAGAGGAGCCCCAT

CGAGGACCTGCTGTTCAACAAGGTGACCCTGGCCGACGCCGGCTT

CATCAAGCAGTACGGCGACTGCCTGGGCGACATCGCCGCCAGGGA

CCTGATCTGCGCCCAGAAGTTCAACGGCCTGACCGTGCTGCCCCC

CCTGCTGACCGACGAGATGATCGCCCAGTACACCAGCGCCCTGCT

GGCCGGCACCATCACCAGCGGCTGGACCTTCGGCGCCGGCCCCG

CCCTGCAGATCCCCTTCCCCATGCAGATGGCCTACAGGTTCAACGG

CATCGGCGTGACCCAGAACGTGCTGTACGAGAACCAGAAGCTGAT

CGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGGACAGCCT

GAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGTGGTGA

ACCACAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAGCA

GCAAGTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCA

GGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATC

ACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTG

ATCAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCAC

CAAGATGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTT

CTGCGGCAAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCC

CCACGGCGTGGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGA

GAAGAACTTCACCACCGCCCCCGCCATCTGCCACGACGGCAAGGC

CCACTTCCCCAGGGAGGGCGTGTTCGTGAGCAACGGCACCCACTG

GTTCGTGACCCAGAGGAACTTCTACGAGCCCCAGATCATCACCACC

GACAACACCTTCGTGAGCGGCAACTGCGACGTGGTGATCGGCATC

GTGAACAACACCGTGTACGACCCCCTGCAGCCCGAGCTGGACAGC

TTCAAGGAGGAGCTGGACAAGTACTTCAAGAACCACACCAGCCCC

GACGTGGACCTGGGCGACATCAGCGGCATCAACGCCAGCGTGGTG

AACATCCAGAAGGAGATCGACAGGCTGAACGAGGTGGCCAAGAAC

CTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGGCAAGTACGAG

CAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTTCATCGCCG

GCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTGCATGAC

CAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGGCAGCTG

CTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGCGT

GAAGCTGCACTACACCTGATGA

29
Spike (6
ATGTTTGTATTCCTGGTACTGCTGCCCCTGGTCAGTAGTCAGTGTG
383

proline
TCAATCTGATCACTCGCACGCAAAGTTATACCAATTCCTTCACTCGC

substitute)
GGTGTCTACTACCCAGACAAGGTGTTTAGGTCCAGCGTGCTCCACA

(codon
GCACACAAGACTTGTTCTTACCATTTTTCAGTAATGTTACCTGGTTT

optimized)
CATGCCATCTCCGGCACAAATGGGACCAAGAGGTTCGACAATCCTG

TGCTACCCTTCAACGACGGGGTGTACTTTGCTTCCACTGAGAAATC

TAATATCATTAGAGGCTGGATCTTTGGTACCACATTAGATTCTAAAA

CTCAGTCACTGCTAATTGTGAATAACGCCACGAACGTCGTGATCAA

GGTCTGTGAGTTTCAATTCTGCAACGACCCTTTCCTGGATGTCTATT

ATCACAAGAACAATAAGAGCTGGATGGAGTCTGAGTTCCGAGTGTA

CAGTTCTGCAAACAATTGCACCTTTGAGTACGTAAGTCAGCCTTTTC

TCATGGATCTGGAAGGCAAGCAGGGTAATTTCAAGAATCTGCGGGA

ATTCGTGTTCAAGAACATTGACGGATATTTCAAAATATATTCAAAGC

ATACACCGATTAACTTGGGGCGGGATCTGCCACAAGGGTTTAGTGC

CCTGGAGCCCTTGGTGGACTTGCCCATTGGCATCAACATTACCCGT

TTTCAGACCCTTCTGGCATTGCACAGGTCTTATCTCACACCTGGGG

ATTCCAGCAGTGGCTGGACAGCCGGTGCAGCTGCCTACTACGTGG

GCTACCTTCAGCCACGTACATTCCTCCTAAAATATAACGAAAACGG

GACCATTACTGATGCAGTCGACTGTGCACTGGACCCACTGTCCGAG

ACTAAATGCACATTAAAGAGCTTCACTGTGGAAAAGGGGATTTACCA

GACGTCCAACTTCAGGGTTCAGCCCACCGAAAGCATCGTCCGCTTC

CCAAATATCACCAACCTGTGTCCCTTTGATGAGGTGTTCAACGCCA

CACGTTTTGCTAGCGTCTATGCCTGGAACCGGAAAAGAATCTCGAA

CTGTGTGGCAGACTACTCAGTGCTCTACAACTTTGCTCCTTTTTTCG

CCTTCAAATGTTACGGCGTTTCACCTACGAAACTGAATGACCTCTGC

TTTACCAATGTCTATGCTGATTCATTTGTGATCCGCGGCAATGAGGT

CAGTCAAATAGCACCCGGGCAGACTGGGAACATAGCCGATTACAAT

TACAAACTACCGGATGACTTCACAGGCTGCGTTATTGCATGGAATT

CGAATAAGTTGGACTCAAAGGTTGGAGGAAATTATAACTACCGGTA

CAGACTGTTCCGTAAGTCTAATCTGAAGCCCTTCGAACGGGACATT

TCCACAGAGATCTACCAGGCTGGAAACAAGCCATGCAATGGCGTG

GCAGGTGTGAACTGCTACTTCCCTCTGCGCTCGTATGGGTTTAGAC

CCACGTATGGAGTGGGACATCAGCCTTATAGAGTTGTGGTGTTAAG

CTTTGAACTGCTTCATGCCCCAGCCACCGTGTGTGGGCCAAAGAAG

TCTACTAACCTTGTTAAAAACAAGTGTGTGAATTTTAATTTTAATGGA

CTGACCGGAACTGGCGTCCTGACTGAGAGCAACAAGAAATTTCTCC

CCTTCCAGCAGTTTGGAAGGGATATTGCTGACACAACCGATGCGGT

CCGCGACCCGCAGACGCTTGAAATTCTGGACATCACCCCCTGCTCT

TTCGGTGGTGTGTCTGTCATCACGCCTGGCACTAACACATCCAACC

AAGTGGCTGTCCTCTATCAGGGAGTTAACTGTACCGAGGTTCCTGT

GGCCATCCACGCGGACCAGCTGACTCCAACATGGCGCGTTTACAG

CACTGGCTCAAACGTATTCCAGACTCGGGCCGGTTGTTTAATTGGT

GCCGAATATGTTAACAACAGTTACGAATGTGATATCCCCATTGGCG

CGGGAATCTGTGCGAGCTACCAAACACAGACTAAGTCCCACCAGCA

GGCCCAGAGTGTGGCTAGCCAGAGCATCATTGCATACACCATGTCC

CTCGGCGCTGAAAATAGTGTTGCTTATTCGAATAATTCCATTGCAAT

TCCTACTAACTTCACCATAAGCGTCACGACAGAAATCCTGCCTGTGT

CTATGACAAAAACATCCGTCGACTGCACGATGTACATCTGCGGCGA

CAGCACAGAGTGCTCAAACCTCCTTCTGCAGTACGGATCTTTCTGT

ACACAACTCAAGAGGGCTCTGACAGGGATCGCAGTGGAACAAGAT

AAGAATACACAGGAAGTTTTCGCGCAGGTGAAACAGATCTACAAGA

CCCCCCCTATTAAGTATTTCGGCGGGTTCAACTTTAGCCAGATTTTG

CCAGATCCGAGCAAACCTAGTAAACGATCCCCTATTGAGGACCTGC

TCTTTAACAAAGTGACATTGGCAGATGCTGGATTTATCAAACAGTAC

GGAGACTGCCTGGGAGACATCGCAGCTCGGGATCTTATCTGTGCA

CAGAAGTTCAATGGTCTTACAGTGCTTCCTCCACTTCTGACAGACG

AGATGATCGCTCAGTATACAAGTGCTCTCCTTGCGGGCACAATAAC

TAGCGGATGGACGTTTGGGGCTGGACCTGCGCTACAGATCCCATTT

CCAATGCAGATGGCCTACAGATTCAACGGGATAGGTGTTACTCAGA

ATGTCCTCTATGAGAACCAGAAATTAATTGCCAACCAGTTCAACTCC

GCAATCGGGAAGATCCAGGATTCACTCTCCAGCACCCCTTCTGCCC

TTGGTAAATTACAAGATGTGGTTAATCATAACGCCCAAGCCCTCAAT

ACACTAGTGAAGCAACTCAGTAGTAAGTTTGGAGCAATATCATCTGT

GCTAAATGATATCTTGTCTCGGTTGGACCCACCTGAGGCAGAAGTG

CAGATAGACAGGCTCATCACTGGGAGGTTACAATCACTTCAGACCT

ATGTCACTCAACAGCTCATCCGAGCTGCCGAGATTAGAGCTAGCGC

CAACCTCGCTGCCACCAAAATGTCTGAATGTGTGTTGGGGCAGTCT

AAACGAGTTGACTTTTGTGGCAAGGGCTACCACCTGATGAGCTTTC

CCCAGTCAGCACCACATGGGGTAGTTTTTTTGCATGTCACCTACGT

ACCCGCCCAGGAGAAGAATTTCACGACCGCTCCCGCTATATGCCAT

GACGGCAAGGCCCACTTTCCAAGAGAGGGAGTGTTCGTTAGCAAC

GGAACCCACTGGTTCGTAACTCAGAGAAACTTCTACGAACCACAGA

TCATCACTACCGATAACACCTTCGTGTCTGGAAACTGTGACGTGGT

GATTGGTATAGTCAACAACACAGTATATGATCCCCTCCAACCGGAG

CTCGATTCATTTAAGGAGGAGCTGGACAAGTACTTTAAAAATCACAC

TTCGCCGGACGTCGACCTTGGTGATATTTCAGGGATCAATGCCTCC

GTAGTAAACATACAGAAGGAAATTGACCGATTGAATGAGGTAGCGA

AAAACCTGAATGAGTCCCTGATAGATCTCCAGGAGCTTGGCAAATA

TGAACAGTATATTAAGTGGCCGTGGTACATCTGGCTGGGCTTCATC

GCCGGCCTGATAGCCATTGTGATGGTGACAATCATGCTGTGCTGCA

TGACCTCTTGCTGCAGCTGCCTGAAGGGCTGCTGTAGTTGTGGGTC

CTGTTGCAAGTTTGATGAGGATGATAGCGAGCCAGTCCTGAAAGGA

GTGAAGCTGCACTATACTTGATAA

In some embodiments, the large sequences are not limited to the above-mentioned conserved fragments.

In certain embodiments, the large sequence comprises spike glycoprotein (S) or a portion thereof (e.g., the RBD), nucleoprotein or a portion thereof, membrane protein or a portion thereof, and/or ORF1a/b or a portion thereof (see Table 9, SEQ ID NO: 139). In certain embodiments, the large sequence comprises Spike glycoprotein (S) or a portion thereof (e.g., the RBD), Nucleoprotein or a portion thereof, and ORF1a/b or a portion thereof. In further embodiments, the large sequence comprises Spike glycoprotein (S) or a portion thereof (e.g., the RBD), and Nucleocapsid protein or a portion thereof (see Table. 9, SEQ ID NO: 140).

In some embodiments, the large sequence(s) are derived from a full-length spike glycoprotein. In other embodiments, the large sequence(s) are derived from a portion of the spike glycoprotein. In some embodiments, the transmembrane anchor of the spike protein has an intact S1-S2 cleavage site. In some embodiments, the spike protein is in its stabilized conformation. In some embodiments, the spike protein is stabilized with proline substitutions at amino acid positions 986 and 987 at the top of the central helix in the S2 subunit. In some embodiments, the composition comprises a SARS-CoV-2 receptor-binding domain (RBD). In some embodiments, the composition comprises a trimerized SARS-CoV-2 receptor-binding domain (RBD). In some embodiments, the trimerized SARS-CoV-2 receptor-binding domain (RBD) sequence is modified by the addition of a T4 fibritin-derived foldon trimerization domain. In some embodiments, the addition of a T4 fibritin-derived foldon trimerization domain increases immunogenicity by multivalent display.

In some embodiments, the spike protein comprises Tyr-489 and Asn-487 (e.g., Tyr-489 and Asn-487 help with interaction with Tyr 83 and Gln-24 on ACE-2). In some embodiments, the spike protein comprises Gln-493 (e.g., Gln-493 helps with interaction with Glu-35 and Lys-31 on ACE-2). In some embodiments, the spike protein comprises Tyr-505 (e.g., Tyr-505 helps with interaction with Glu-37 and Arg-393 on ACE-2). In some embodiments, the composition comprises a mutation 682-RRAR-685→682-QQAQ-685 in the S1-S2 cleavage site.

In some embodiments, the spike protein comprising the large sequence(s) comprises at least one proline substitution. In some embodiments, the spike protein comprising the large sequence(s) comprises at least two proline substitutions. For example, the proline substitution may be at position K986 and V987.

Non-limiting examples of Spike protein sequences are disclosed in Table 2.

TABLE 2

SEQ ID

Sequence:
NO:

SARS-CoV-like
SQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPF
186

Spike-S1-NTD
FSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGW

13 bp-304 bp
FGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSW

MESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNID

GYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHR

SYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCAL

DPLSETKCTLK

SARS-CoV-2
RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADY
187

Spike-S-RBD
SVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAP

319 bp-541 bp
GQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRK

SNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVG

YQPYRVWVLSFELLHAPATVCGPKKSTNLVKNKCVNF

CoV Spike S1-
FNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVTRAGCLIGAEHVN
188

S2_S2
NSYECDIPIGAGICASYQTQTNRDPQTLEILDITPCSFGGVSVITPGT

543 bp-1, 208 bp
NTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQSPR

RARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSM

TKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNT

QEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKV

TLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYT

SALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQ

KLIANQFNSAIGKIQDSLSSTASALGKLQDWNQNAQALNTLVKQLS

SNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAA

EIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGW

FLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQR

NFYEPQIITTDNTFVSGNCDWIGIVNNTVYDPLQPELDSFKEELDKY

FKNHTSPDVDLGDISGINASWNIQKEIDRLNEVAKNLNESLIDLQEL

GKYEQ

spike glycoprotein
MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSS
189

with a mutation
VLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYF

682-RRAR-685-
ASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNWIKVCEFQFCNDPF

682-QQAQ-685 in
LGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQG

the S1-S2
NFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGI

cleavage site
NITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKY

NENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIV

RFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSAS

FSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIAD

YNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFER

DISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVV

LSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKK

FLPFQQFGRDIADTTDAVTRAGCLIGAEHVNNSYECDIPIGAGICASY

QTQTNRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCT

EVPVAIHADQLTPTWRVYSTGSNVFQSPQQAQSVASQSIIAYTMSL

GAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDST

ECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPI

KDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCL

GDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFG

AGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQD

SLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSR

LDKVEAEVQIDRLITGRLQSLQTYVTOQLIRAAEIRASANLAATKMSE

CVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFT

TAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVS

GNCDWIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDIS

GINASVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWIWL

GFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVL

KGVKLHYT

spike glycoprotein
MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSS
190

with two proline
VLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYF

substitutions
ASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNWIKVCEFQFCNDPF

(K986P, V987P)
LGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQG

NFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGI

NITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKY

NENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIV

RFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSAS

FSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIAD

YNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFER

DISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRWV

LSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKK

FLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSN

QVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIG

AEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSL

GAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDST

ECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPI

KDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCL

GDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFG

AGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQD

SLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSR

LDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSE

CVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFT

TAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVS

GNCDWIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDIS

GINASVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWL

GFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVL

KGVKLHYT

spike glycoprotein
MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSS
191

with four proline
VLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYF

substitutions
ASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPF

(F817P, A892P,
LGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQG

A899P, A942P)
NFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGI

NITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKY

NENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIV

RFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSAS

FSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIAD

YNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFER

DISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRV

LSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKK

FLPFQQFGRDIADTTDAVTRAGCLIGAEHVNNSYECDIPIGAGICASY

QTQTNRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCT

EVPVAIHADQLTPTWRVYSTGSNVFQSPRRARSVASQSIIAYTMSL

GAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDST

ECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPI

KDFGGFNFSQILPDPSKPSKRSPIEDLLFNKVTLADAGFIKQYGDCL

GDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFG

AGPALQIPFPMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQD

SLSSTPSALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSR

LDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSE

CVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFT

TAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVS

GNCDWIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDIS

GINASVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWL

GFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVL

KGVKLHYT

spike glycoprotein
MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSS
192

with six proline
VLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYF

substitutions
ASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNWIKVCEFQFCNDPF

(F817P, A892P,
LGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQG

A899P, A942P,
NFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGI

K986P, V987P)
NITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKY

NENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIV

RFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSAS

FSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIAD

YNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFER

DISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRWV

LSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKK

FLPFQQFGRDIADTTDAVTRAGCLIGAEHVNNSYECDIPIGAGICASY

QTQTNRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCT

EVPVAIHADQLTPTWRVYSTGSNVFQSPRRARSVASQSIIAYTMSL

GAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDST

ECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPI

KDFGGFNFSQILPDPSKPSKRSPIEDLLFNKVTLADAGFIKQYGDCL

GDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFG

AGPALQIPFPMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQD

SLSSTPSALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSR

LDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSE

CVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFT

TAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVS

GNCDWIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDIS

GINASWNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWL

GFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVL

KGVKLHYT

spike glycoprotein
MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSS
193

with six proline
VLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYF

substitutions
ASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNWIKVCEFQFCNDPF

(F817P, A892P,
LGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQG

A899P, A942P,
NFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGI

K986P, V987P)
NITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKY

and a 682-RRAR-
NENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIV

685 - 682-QQAQ-
RFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSAS

685 mutation
FSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIAD

YNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFER

DISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVV

LSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKK

FLPFQQFGRDIADTTDAVTRAGCLIGAEHVNNSYECDIPIGAGICASY

QTQTNRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCT

EVPVAIHADQLTPTWRVYSTGSNVFQSPQQAQSVASQSIIAYTMSL

GAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDST

ECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPI

KDFGGFNFSQILPDPSKPSKRSPIEDLLFNKVTLADAGFIKQYGDCL

GDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFG

AGPALQIPFPMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQD

SLSSTPSALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSR

LDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSE

CVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFT

TAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVS

GNCDWIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDIS

GINASWNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWL

GFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVL

KGVKLHYT

Wild type native
MFVFLVLLPLVSS
63

leader sequence

Depending on the specific mutation and wherein the genome the nucleotide substitution, addition or deletion occurs, mutations may be neutral, beneficial, or harmful to an organism. Spike (S) protein of SARS-CoV-2 is 1273 amino acids long and is the main target of current COVID-19 vaccines, as well as those in development. It is the portion of the virus that recognizes and binds to host cellular receptors and mediates viral entry. SARS-CoV-2 is unable to infect host cells without it. Because of this, mutations in the S gene, particularly those that affect portions of the protein that are critical for pathogenesis and normal function (such as the receptor-binding domain (RBD) or furin cleavage site) or those that cause conformational changes to the S protein, are of the most significant interest. If “first-wave” antibodies do not recognize these changes, these mutations may provide an avenue for the virus to escape from immunity to the original SARS-CoV-2 strain. In the current study, using robust immunoinformatic approaches, potential human B cell, CD8⁺, and CD4⁺ T cell-specific epitopes were identified in context to a spike glycoprotein region substituted with all the known 36 non-synonymous mutations that constitute the 17 SARS-CoV-2 specific Variants of Concern (VOC). Usage of these epitopes in a multi-epitope vaccine candidate will aid in combating a higher degree of hospitalization and deaths caused due to the highly severe SARS-CoV-2 VOCs and bring a curb on the pandemic.

As previously discussed, each of the large sequences are separated by a linker. In some embodiments, the linker is the same linker. In some embodiments, one or more linkers are different. For example, in some embodiments, a different linker is used between each large sequence. As previously discussed, non-limiting examples of linkers include T2A, E2A, P2A, or the like.

As previously discussed, in certain embodiments, the vaccine delivery system comprises an adenovirus such as but not limited to Ad5. Ad26, Ad35, etc., as well as carriers such as lipid nanoparticles, polymers, peptides, etc.

CD8+ Epitopes

Examples of methods for identifying potential CD8+ T cell epitopes and screening conservancy of potential CD8+ T cell epitopes are described herein. The present invention is not limited to the particular software systems disclosed, and other software systems are accessible to one of ordinary skill in the art for such methods. The present invention is not limited to the specific haplotypes used herein. For example, one of ordinary skill in the art may select alternative molecules (e.g., HLA molecules) for molecular docking studies,

FIG. 10 shows sequence homology analysis for screening conservancy of potential CD8+ T cell epitopes, e.g., the comparison of sequence homology for the potential CD8+ T cell epitopes among 81,963 SARS-CoV-2 strains (that currently circulate in 190 countries on 6 continents), the 4 major “common cold” Coronaviruses that cased previous outbreaks (e.g. hCoV-OC43, hCoV-229E, hCoV-HKU1-Genotype B, and hCoV-NL63), and the SL-CoVs that were isolated from bats, civet cats, pangolins and camels. Epitope sequences highlighted in yellow present a high degree of homology among the currently circulating 81,963 SARS-CoV-2 strains and at least a 50% conservancy among two or more humans SARS-CoV strains from previous outbreaks, and the SL-CoV strains isolated from bats, civet cats, pangolins and camels.

From the analysis, 27 CD8+ T cell epitopes were selected as being highly conserved. FIG. 11A and FIG. 11B show the docking of the conserved epitopes to the groove of HLA-A*02:01 molecules as well as the interaction scores determined by protein-peptide molecular docking analysis.

FIG. 12A, FIG. 12B, and FIG. 12C shows that CD8+ T cells specific to several highly conserved SARS-CoV-2 epitopes disclosed herein were detected in COVID-19 patients and unexposed healthy individuals. FIG. 13A, FIG. 13B, FIG. 13C, and FIG. 13D shows immunogenicity of the identified SARS-CoV-2 CD8+ T cell epitopes.

The CD8⁺ T cell target epitopes discussed above include S_2-10, S_1220-1228, S_1000-1008, S_958-966, E_20-28, ORF1ab_1675-1683, ORF1ab_2363-2371, ORF1ab_3013-3021, ORF1ab_3133-3191, ORF1ab_5470-5478, ORF1ab_6749-6757, ORF7b_26-34, ORF8a_73-81, ORF10_3-11, and ORF10_5-13. FIG. 14 shows the genome-wide location of the epitopes. Thus, in certain embodiments, the vaccine composition may comprise one or more CD8⁺ T cell epitopes selected from: S_2-10, S_1220-1228, S_1000-1008, S_958-986, E_20-28, ORF1ab_1675-1683, ORF1ab_2363-2371, ORF1ab_3013-3021, ORF1ab_3183-3191, ORF1ab_5470-5478, ORF1ab_6749-6757, ORF7b_26-34, ORF8a_73-81, ORF10_3-11, ORF10_5-13, or a combination thereof. Table 3 below describes the sequences for the aforementioned epitope regions.

TABLE 3

SEQ

CD8⁺ T Cell
Epitope
ID

Epitope
Sequence
NO:

ORF1ab_84-92
VMVELVAEL
2

ORF1ab_1675-1583
YLATALLTL
3

ORF1ab_2210-2218
CLEASFNYL
4

ORF1ab_2383-2371
WLMWLIINL
5

ORF1ab_3013-3021
SLPGVFCGV
6

ORF1ab_3183-3191
FLLNKEMYL
7

ORF1ab_3732-3740
SMWALIISV
8

ORF1ab_4283-4291
YLASGGQPI
9

ORF1ab_5470-5478
KLSYGIATV
10

ORF1ab_6419-6427
YLDAYNMMI
11

ORF1ab_6749-B757
LLLDDFVEI
12

S_2-10
FVFLVLLPL
13

S_691-699
SIIAYTMSL
14

S_958-956
ALNTLVKQL
15

S_976-984
VLNDILSRL
16

S_1000-1008
RLQSLQTYV
17

S_1220-1228
FIAGLIAIV
18

E_20-28
FLAFVVFLL
19

E_25-34
FLLVTLAIL
20

E_26-34
FLLNKEMYL
21

M_52-60
IFLWLLWPV
22

M_89-97
GLMWLSYFI
23

ORF6_3-11
HLVDFQVTI
24

ORF7b_26-34
IIFWFSLEL
25

ORF8a_31-39
YVVDDPCPI
26

ORF8a_73-81
YIDIGNYTV
27

ORF10_3-11
YINVFAFPF
28

ORF10_5-13
NVFAFPFTL
29

S
KSYGFQPTY
194

S
VVGNHKYRF
195

S
YQVGNKPCK
196

S
CVIAWNSKK
137

S
KGAKGLNCY
198

S
SQCVNFTTR
199

S
NIADYNYKL
200

S
YLPLKSYGF
201

S
KCYGVSLNK
202

S
IYKTPPIKY
203

S
CVADYSFLY
204

S
SVYAWDRRK
205

S
RFFRKSNLK
206

S
DiSTEIYQV
207

S
YQPHRVWVL
208

S
FVIRGDQVK
209

S
NATKFSSVY
210

S
NLCPFSEIF
211

S
KINNCVADY
213

The present invention is not limited to the aforementioned CD8⁺ T cell epitopes. For example, the present invention also includes variants of the aforementioned CD8⁺ T cell epitopes, for example sequences wherein the aforementioned CD8⁺ T cell epitopes are truncated by one amino acid (examples shown below in Table 4).

TABLE 4

CD8⁺TCell
Sequence with

Epitope
Single AA
SEQ ID

Origin:
Truncation
NO:

ORF1ab_84-92
VMVELVAE
30

ORF1ab_1675-1583
LATALLTL
31

ORFIab_2210-2218
CLEASFNY
32

ORFIab_2383-2371
LMWLIINL
33

ORFIab_3013-3024
SLPGVFCG
34

ORFIab_3183-3191
LLNKEMYL
35

ORF1ab_3732-3740
SMWALIIS
36

ORF1ab_4283-4291
LASGGQPI
37

ORF1ab_5470-5478
KLSYGIAT
38

ORF1ab_6419-5427
LDAYNMMI
39

ORF1ab_6749-8757
LLLDDFVE
40

S_2-10
VFLVLLPL
41

S_691-699
SIIAYTMS
42

S_958-966
LNTLVKQL
43

S_976-984
VLNDILSR
44

S_1000-1008
LQSLQTYV
45

S_1220-1228
FIAGLIAI
46

E_20-28
LAFVVFLL
47

E_28-34
FLLVTLAL
48

E_28-34
LLNKEMYL
49

M_52-60
IFLWLLWP
59

M_89-97
LMWLSYFI
51

ORF_63-11
HLVDFQVT
52

ORF7b_28-34
IFWFSLEL
53

ORF8a_31-39
YVVDDPCP
54

ORF8a_73-81
iDIGNYTV
55

ORF10_3-11
YINVFAFP
56

ORF10_5-13
VFAFPFTI
57

S
KSYGFQPT
214

S
VVGNHKYR
215

S
YQVGNKPC
216

S
CVIAWNSK
217

S
KGAKGLNC
218

S
SQCVNFTT
219

S
NIADYNYK
220

S
YLPLKSYG
221

S
KCYGVSLN
222

S
IYKTPPIK
223

S
CVADYSFL
224

S
SVYAWDRR
225

S
RFFRKSNL
226

S
DiSTEIYQ
227

S
YQPHRVVV
228

S
FVIRGDQV
229

S
NATKFSSV
230

S
NLCPFSEI
231

S
ASATVCGP
232

S
KINNCVAD
233

S
KSYGFQPT
234

The present invention is not limited to the aforementioned CD8⁺ T cell epitopes.

In certain embodiments, the vaccine composition comprises 1-10 CD8⁺ T cell target epitopes. In certain embodiments, the vaccine composition comprises 2-10 CD8⁺ T cell target epitopes. In certain embodiments, the vaccine composition comprises 2-15 CD8⁺ T cell target epitopes. In certain embodiments, the vaccine composition comprises 2-20 CD8⁺ T cell target epitopes. In certain embodiments, the vaccine composition comprises 2-30 CD8⁺ T cell target epitopes. In certain embodiments, the vaccine composition comprises 2-15 CD8⁺ T cell target epitopes. In certain embodiments, the vaccine composition comprises 2-5 CD8⁺ T cell target epitopes. In certain embodiments, the vaccine composition comprises 5-10 CD8⁺ T cell target epitopes. In certain embodiments, the vaccine composition comprises 5-15 CD8⁺ T cell target epitopes. In certain embodiments, the vaccine composition comprises 5-20 CD8⁺ T cell target epitopes. In certain embodiments, the vaccine composition comprises 5-25 CD8⁺ T cell target epitopes. In certain embodiments, the vaccine composition comprises 5-30 CD8⁺ T cell target epitopes. In certain embodiments, the vaccine composition comprises 10-20 CD8⁺ T cell target epitopes. In certain embodiments, the vaccine composition comprises 10-30 CD8⁺ T cell target epitopes

CD4+ Epitopes

Examples of methods for identifying potential CD4+ T cell epitopes and screening conservancy of potential CD4+ T cell epitopes are described herein. The present invention is not limited to the particular software systems disclosed, and other software systems are accessible to one of ordinary skill in the art for such methods. The present invention is not limited to the specific haplotypes used herein. For example, one of ordinary skill in the art may select alternative molecules (e.g., HLA molecules) for molecular docking studies,

FIG. 15 shows the identification of highly conserved potential SARS-CoV-2-derived human CD4+ T cell epitopes that bind with high affinity to HLA-DR molecules. Out of a total of 9,594 potential HLA-DR-restricted CD4+ T cell epitopes from the whole genome sequence of SARS-CoV-2-Wuhan-Hu-1 strain (MN908947.3), 16 epitopes that bind with high affinity to HLA-DRB1 molecules were selected. The conservancy of the 16 CD4+ T cell epitopes was analyzed among human and animal Coronaviruses Shown are the comparison of sequence homology for the 16 CD4+ T cell epitopes among 81,963 SARS-CoV-2 strains (that currently circulate in 6 continents), the 4 major “common cold” Coronaviruses that cased previous outbreaks (i.e. hCoV-OC43, hCoV-229E, hCoV-HKU1, and hCoV-NL63), and the SL-CoVs that were isolated from bats, civet cats, pangolins and camels. Epitope sequences highlighted in green present high degree of homology among the currently circulating 81,963 SARS-CoV-2 strains and at least a 50% conservancy among two or more humans SARS-CoV strains from previous outbreaks, and the SL-CoV strains isolated from bats, civet cats, pangolins and camels.

From the analysis, 16 CD4+ T cell epitopes were selected as being highly conserved. FIG. 16A and FIG. 16B show the docking of the conserved epitopes to the groove of HLA-A*02:01 molecules as well as the interaction scores determined by protein-peptide molecular docking analysis.

FIG. 17A, FIG. 17B, and FIG. 17C show that CD4+ T cells specific to several highly conserved SARS-CoV-2 epitopes disclosed herein were detected in COVID-19 patients and unexposed healthy individuals. FIG. 18A, FIG. 18B, FIG. 18C, and FIG. 18D show immunogenicity of the identified SARS-CoV-2 CD4+ T cell epitopes.

The CD4⁺ T cell target epitopes discussed above include ORF1a_1350-1365, ORF1ab_5019-5033, ORF6_12-26, ORF1ab_6086-6102, ORF1ab_6420-6434, ORF1a_1801-1815, S_1-13, E_26-40, E_20-34, M_176-190, N_288-403, ORF7a_3-17, ORF7a_1-15, ORF7b_8-22, ORF7a_96-112, and ORF8_1-15. FIG. 14 shows the genome-wide location of the epitopes. Thus, in certain embodiments, the vaccine composition may comprise one or more CD4⁺ T cell target epitopes selected from ORF1a_1350-1365, ORF1ab_5019-5033, ORF6_12-26, ORF1ab_6086-6102, ORF1ab_6420-6434, ORF1a_1801-1815, S_1-13, E_26-40, E_20-34, M_176-190, N_388-403, ORF7a_3-17, ORF7a_1-15, ORF7b_8-22, ORF7a_98-112, ORF8_1-15, or a combination thereof. Table 5 below describes the sequences for the aforementioned epitope regions.

TABLE 5

CD4⁺

SEQ

T Cell

ID

Epitope
Epitope Sequence
NO:

ORF1a_1350-1385
KSAFYILPSIISNEK
58

ORF1a_1801-1815
ESPFVMMSAPPAQYE
59

ORF1ab_5019-5033
PNMLRIMASLVLARK
60

ORF1ab_6088-6102
RIKVQMLSDTLKNL
61

ORF1ab_6420-6434
LDAYNMMISAGFSLW
62

S_1-13
MFVFLVLLPLVSS
63

E_20-34
FLAFVVFLLVTLAIL
64

E_28-40
FLLVTLAILTALRLC
65

M_175-190
LSYYKLGASQRVAGD
66

ORF6_l2-26
AEILLIIMRTFKVSI
67

ORF7a_1-15
MKIILFLALITLATC
68

ORF7a_3-17
IIFLALITLATCEL
69

ORF7a_96-112
SPIFLIVAAIVFITL
70

ORF7b_8-22
DFYLCFLAFLLFLVL
71

ORFBb_1-15
MKFLVFLGIITTVAA
72

N_388-4031
KQQTVTLLPAADLDDF
73

S
LCPFSEIFNATKFSS
235

S
NCYLPLKSYGFQPTY
236

S
GNHKYRFRFFRKSNL
237

S
PFERDISTEIYQVGN
238

S
KKLDSKVVGNHKYRF
239

S
KGLNCYLPLKSYGFQ
240

S
LVLLPLVSSQCVNFT
241

S
RGDQVKQIAPGQTGN
242

S
SASFSTFKCYGVSLN
243

S
KLDSKVVGNHKYRFR
244

S
FAQVKQIYKTPPIKY
245

S
ADYSFLYNSASFSTF
246

S
ATKFSSVYAWDRRKI
247

S
PHRVVVLSFELLHAS
248

S
FERDISTEIYQVGNK
249

S
AKGLNCYLPLKSYGF
250

S
SIVRFPNITNLCPFS
251

S
NNCVADYSFLYNSAS
252

S
KGAKGLNCYLPLKSY
253

The present invention is not limited to the aforementioned CD4⁺ T cell epitopes. For example, the present invention also includes variants of the aforementioned CD4⁺ T cell epitopes, for example sequences wherein the aforementioned CD42 T cell epitopes are truncated by one or more amino acids or extended by one or more amino acids (examples shown below in Table 6).

TABLE 6

CD4⁺ T Cell
Sequence
SEQ

Epitope
with Single
ID

Origin
AA Truncation
NO:

ORF1a_1350-1385
KSAFYILPSIISNE
74

ORF1a_1801-1815
ESPFVMMSAPPAQY
75

ORF1ab_5019-5033
PNMLRIMASLVLAR
76

ORF1ab_6088-8102
RIKVQMLSDTLKN
77

ORF1ab_6420-8434
LDAYNMMISAGFSL
78

S_1-13
MFVFLVLLPLVS
79

E_20-34
FLAFVVFLLVTLAL
80

E_28-40
FLLVTLAILTALRL
81

M_175-190
LSYYKLGASQRVAG
82

ORF6_12-26
AEILLIIMRTFKVS
83

ORF7a_1-15
MKIILFLALITLAT
84

ORF7a_3-17
IFLALITLATCE
85

ORF7a_98-112
SPIFLIVAAIVFIT
86

ORF7b₈-₂₂
DFYLCFLAFLLFLV
87

ORF8b_1-15
MKFLVFLGIITTVA
88

N_388-4031
KQQTVTLLPAADLDD
89

ORF1a_1250-1385
SAFYILPSIISNEK
90

ORF1a_1801-1815
SPFVMMSAPPAQYE
91

ORF1ab_5019-5033
NMLRIMASLVLARK
92

ORF1ab_6088-6102
IKVQMLSDTLKNL
93

ORF1ab_6420-6434
DAYNMMISAGFSLW
94

S_1-13
FVFLVLLPLVSS
95

E_20-34
LAFVVFLLVTLAIL
96

E_28-40
LLVTLAILTALRLC
97

M_176-190
SYYKLGASQRVAGD
98

ORF6_12-26
EILLI IMRTFKVSI
99

ORF7a_1-15
KIILFLALITLATC
100

ORF7a_3-17
IFLALITLATCEL
101

ORF7a_98-112
PIFLIVAAIVFITL
102

ORF7b_8-22
FYLCFLAFLLFLVL
103

ORF8b1-15
KFLVFLGIITTVAA
104

N_388-4031
QQTVTLLPAADLDDF
105

S
LCPFSEIFNATKFS
254

S
NCYLPLKSYGFQPT
255

S
GNHKYRFRFFRKSN
256

S
PFERDISTEIYQVG
257

S
KKLDSKVVGNHKYR
258

S
KGLNCYLPLKSYGF
259

S
LVLLPLVSSQCVNF
260

S
RGDQVKQIAPGQTG
261

S
SASFSTFKCYGVSL
262

S
KLDSKWGNHKYRF
263

S
FAQVKQIYKTPPIK
264

S
ADYSFLYNSASFST
265

S
ATKFSSVYAWDRRK
266

S
PHRVVVLSFELLHA
267

S
FERDISTEIYQVGN
268

S
AKGLNCYLPLKSYG
269

S
SIVRFPNITNLCPF
270

S
NNCVADYSFLYNSA
271

S
KGAKGLNCYLPLKS
272

The present invention is not limited to the aforementioned CD4⁺ T cell epitopes.

In certain embodiments, the vaccine composition comprises 1-10 CD4⁺ T cell target epitopes. In certain embodiments, the vaccine composition comprises 2-10 CD4⁺ T cell target epitopes. In certain embodiments, the vaccine composition comprises 2-15 CD4⁺ T cell target epitopes. In certain embodiments, the vaccine composition comprises 2-20 CD4⁺ T cell target epitopes. In certain embodiments, the vaccine composition comprises 2-30 CD4⁺ T cell target epitopes. In certain embodiments, the vaccine composition comprises 2-15 CD4⁺ T cell target epitopes. In certain embodiments, the vaccine composition comprises 2-5 CD4⁺ T cell target epitopes. In certain embodiments, the vaccine; composition comprises 5-10 CD4⁺ T cell target epitopes. In certain embodiments, the vaccine composition comprises 5-15 CD4+ T cell target epitopes. In certain embodiments, the vaccine composition comprises 5-20 CD4⁺ T cell target epitopes. In certain embodiments, the vaccine composition comprises 5-25 CD4⁺ T cell target epitopes. In certain embodiments, the vaccine composition comprises 5-30 CD4⁺ T cell target epitopes. In certain embodiments, the vaccine composition comprises 10-20 CD4⁺ T cell target epitopes. In certain embodiments, the vaccine composition comprises 10-30 CD4⁺ T cell target epitopes.

B Cell Epitopes

Examples of methods for identifying potential B cell epitopes and screening conservancy of potential B cell epitopes are described herein. The present invention is not limited to the particular software systems disclosed, and other software systems are accessible to one of ordinary skill in the art for such methods.

FIG. 19 shows the conservation of Spike-derived B cell epitopes among human, bat, civet cat, pangolin, and camel coronavirus strains. Multiple sequence alignment performed using ClustalW among 29 strains of SARS coronavirus (SARS-CoV) obtained from human, bat, civet, pangolin, and camel. This includes 7 human SARS/MERS-CoV strains (SARS-CoV-2-Wuhan (MN908947.3), SARS-HCoV-Urbani (AY278741.1), CoV-HKU1-Genotype-B (AY884001), CoV-OC43 (KF923903), CoV-NL63 (NC005831), CoV-229E (KY983587), MERS (NC019843)); 8 bat SARS-CoV strains (BAT-SL-CoV-WIV16 (KT444582), BAT-SL-CoV-WIV1 (KF367457.1), BAT-SL-CoV-YNLF31C (KP886808.1), BAT-SARS-CoV-RS672 (FJ588686.1), BAT-CoV-RATG13 (MN996532.1), BAT-CoV-YN01 (EPIISL412976), BAT-CoV-YNO2 (EPIISL412977), BAT-CoV-19-ZXC21 (MG772934.1); 3 Civet SARS-CoV strains (SARS-CoV-Civet007 (AY572034.1), SARS-CoV-A022 (AY686863.1), SARS-CoV-B039 (AY686864.1)); 9 pangolin SARS-CoV strains (PCoV-GX-P2V (MT072864.1), PCoV-GX-P5E (MT040336.1), PCoV-GX-P5L (MT040335.1), PCoV-GX-P1E (MT040334.1), PCoV-GX-P4L (MT040333.1), PCoV-MP789 (MT084071.1), PCoV-GX-P3B (MT072865.1), PCoV-Guangdong-P2S (EPIISL410544), PCoV-Guangdong (EPIISL410721)); 4 camel SARS-CoV strains (Camel-CoV-HKU23 (KT368891.1), DcCoV-HKU23 (MN514967.1), MERS-CoV-Jeddah (KF917527.1), Riyadh/RY141 (NC028752.1)) and 1 recombinant strain (FJ211859.1)). Regions highlighted with blue color represent the sequence homology. The B cell epitopes, which showed at least 50% conservancy among two or more strains of the SARS Coronavirus or possess receptor-binding domain (RBD) specific amino acids were selected as candidate epitopes.

From the analysis, 22 B cell epitopes were selected as being highly conserved. FIG. 20A and FIG. 20B shows the docking of the conserved epitopes to the ACE2 receptor as well as the interaction scores determined by protein-peptide molecular docking analysis. FIG. 21A, FIG. 21B, FIG. 21C, FIG. 21D, FIG. 21E, FIG. 21F, and FIG. 21G shows immunogenicity of the identified SARS-CoV-2 B cell epitopes.

The B cell target epitopes discussed above include S_287-317, S_524-598, S_601-640, S_802-819, S_888-909, S_369-393, S_440-501, S_1133-1172, S_329-363, S_59-81, and S_13-37. FIG. 28 shows the genome-wide location of the epitopes. Thus, in certain embodiments, the vaccine composition may comprise one or more B cell target epitopes selected from: S_237-317, S_524-598, S_601-640, S_802-819, S_888-909, S_359-393, S_440-501, S_1133-1172, S_329-363, S_59-81, and S_13-37. In some embodiments; the B cell epitope is whole spike protein. In some embodiments, the B cell epitope is a portion of the spike protein. Table 7 below describes the sequences for the aforementioned epitope regions.

TABLE 7

SEQ

B Cell

ID

Epitope
Epitope Sequence
NO:

S_13-37
SQCVNLTTRTQLPPAYTNSFT
106

RGVY

S_59-81
FSNVTWFHAIHVSGTNGTKRF
107

DN

S_287-317
DAVDCALDPLSETKCTLKSFT
108

VEKGIYQTSN

S_601-640
GTNTSNQVAVLYODVNCTEV
109

PVAIHADQLTPTWRVYSTGS

S_524-598
VCGPKKSTNLVKNKCVNFNFN
110

GLTGTGVLTESNKKFLPFQQF

GRDIADTTDAVRDPQTLEILDI

TPCSFGGVSVI

S_440-501
NLDSKVGGNYNYLYRLFRKSN
111

LKPFERDISTEIYQAGSTPCNG

VEGFNCYFPLQSYGFQPTE

S_369-393
YNSASFSTFKCYGVSPTKLND
112

LCFT

S_329-363
FPNITNLCPFGEVFNATRFASV
113

YAWNRKRISNCVA

S_1133-1172
VNNTVYDPLQPELDSFKEELD
114

KYFKNHTSPDVDLGDISGI

S_802-819
FSQILPDPSKPSKRSFIE
115

S_888-909
FGAGAALQIPFAMQMAYRFN
116

GI

S
CVNFTTRTQLPPAYTNSFT
273

RGVYY

S
NITNLCPFSEIFNATKFSSV
274

YAWDRR

S
INNCVADYSFLYNSASFST

FKCYGVSLNKLNDL

S
RGDQVKQIAPGQTGNIAD
276

S
KKLDSKWGNHKYRFRFFR
277

KSNLKPFERDISTEISTEIY

QVGNKPCKG

S
TYGVGY
278

S
LHASATVCGPKKSTNL
279

S
VKQIYKTPPIKYFGGFNFS
280

QILPDPSKPSK

The present invention is not limited to the aforementioned B cell epitopes. For example, the present invention also includes variants of the aforementioned B cell epitopes, for example sequences wherein the aforementioned B cell epitopes are truncated by one or more amino acids or extended by one or more amino acids (examples shown below in Table 8).

TABLE 8

Origin

SEQ

of
Sequence with AA
ID

Epitope
Truncation
NO:

S_13-37
SQCVNLTTRTQLPPAYTNSFT
117

RG

S_59-79
FSNVTWFHAIHVSGTNGTKRF
118

S_287-315
DAVDCALDPLSETKCTLKSFT
119

VEKGIYQT

S_601-538
GTNTSNQVAVLYQDVNCTEV
120

PVAIHADQLTPTWRVYST

S_524-596
VCGPKKSTNLVKNKCVNFNFN
121

GLTGTGVLTESNKKFLPFQQF

GRDIADTTDAVRDPQTLEILDI

TPCSFGGVS

S_440-499
NLDSKVGGNYNYLYRLFRKSN
122

LKPFERDISTEIYQAGSTPCNG

VEGFNCYFPLQSYGFQP

S_369-391
YNSASFSTFKCYGVSPTKLND
123

LC

S_329-361
FPNITNLCPFGEVFNATRFASV
124

YAWNRKRISNC

S_1133-1170
VNNTVYDPLQPELDSFKEELD
125

KYFKNHTSPDVDLGDIS

S_802-817
FSQILPDPSKPSKRSF
126

S_888-907
FGAGAALQIPFAMOMAYRFN
127

S_13-37
CVNLTTRTQLPPAYTNSFT
128

RGVY

S_61-81
NVTWFHAIHVSGTNGTKR
129

FDN

S_287-317
VDCALDPLSETKCTLKSFT
130

VEKGIYQTSN

S_601-640
NTSNQVAVLYQDVNCTEV
131

PVAIHADQLTPTWRVYSTG

S

S_524-598
GPKKSTNLVKNKCVNFNF
132

NGLTGTGVLTESNKKFLPF

QQFGRDIADTTDAVRDPQ

TLEILDITPCSFGGVSVI

S_440-501
DSKVGGNYNYLYRLFRKS
133

NLKPFERDISTEIYQAGSTP

CNGVEGFNCYFPLQSYGF

QPTE

S_371-393
SASFSTFKCYGVSPTKLND
134

LCFT

S_331-383
NITNLCPFGEVFNATRFAS
135

VYAWNRKRISNCVA

S_1135-1172
NTVYDPLQPELDSFKEELD
136

KYFKNHTSPDVDLGDISGI

S_804-819
QILPDPSKPSKRSFIE
137

S_890-909
AGAALQIPFAMQMAYRFN
138

GI

S
CVNFTTRTQLPPAYTNSFTRG
281

V

S
NITNLCPFSEIFNATKFSSVYA
282

WD

S
INNCVADYSFLYNSASFSTFK
283

CYGVSLNKLN

S
RGDQVKQIAPGQTGNI
284

S
KKLDSKWGNHKYRFRFFRKS
285

NLKPFERDISTEISTEIYQVGN

KPC

S
LHASATVCGPKKST
286

S
VKQIYKTPPIKYFGGFNFSQIL
287

PDPSKP

S
NFTTRTQLPPAYTNSFTRG
288

VYY

S
TNLCPFSEIFNATKFSSVYA
289

WDRR

S
NCVADYSFLYNSASFSTFK
290

CYGVSLNKLNDL

S
DQVKQIAPGQTGNIAD
291

S
LDSKWGNHKYRFRFFRKS
292

NLKPFERDISTEISTEIYQV

GNKPCKG

S
ASATVCGPKKSTNL
293

S
QIYKTPPIKYFGGFNFSQIL
294

PDPSKPSK

As previously discussed, in some embodiments, the B cell epitope is in the form of whole spike protein. In some embodiments, the B cell epitope is in the form of a portion of spike protein. In some embodiments, the transmembrane anchor of the spike protein has an intact S1-S2 cleavage site. In some embodiments, the spike protein is in its stabilized conformation. In some embodiments, the spike protein is stabilized with proline substitutions at amino acid positions 986 and 987 at the top of the central helix in the S2 subunit. In some embodiments, the composition comprises a trimerized SARS-CoV-2 receptor-binding domain (RBD). In some embodiments, the trimerized SARS-CoV-2 receptor-binding domain (RBD) sequence is modified by the addition of a T4 fibritin-derived foldon trimerization domain. In some embodiments, the addition of a T4 fibritin-derived foldon trimerization domain increases immunogenicity by multivalent display. FIG. 22 shows a non-limiting example of a spike protein comprising one or more mutations.

In some embodiments, the spike protein comprises Tyr-489 and Asn-487 (e.g., Tyr-489 and Asn-487 help with interaction with Tyr 83 and Gln-24 on ACE-2). In some embodiments, the spike protein comprises Gln-493 (e.g., Gln-493 helps with interaction with Glu-35 and Lys-31 on ACE-2). In some embodiments, the spike protein comprises Tyr-505 (e.g., Tyr-505 helps with interaction with Glu-37 and Arg-393 on ACE-2). In some embodiments, the composition comprises a mutation 682-PRAR-685→682-QQAQ-685 in the S1-S2 cleavage site.

In some embodiments, the composition comprises at least one proline substitution. In some embodiments, the composition comprises at least two proline substitutions. For example, the proline substitution may be at position K986 and V987.

In certain embodiments, the vaccine composition comprises 1-10 B cell target epitopes. In certain embodiments, the vaccine composition comprises 2-10 B cell target epitopes. In certain embodiments, the vaccine composition comprises 2-15 B cell target epitopes. In certain embodiments, the vaccine composition comprises 2-20 B cell target epitopes. In certain embodiments, the vaccine composition comprises 2-30 B cell target epitopes. In certain embodiments, the vaccine composition comprises 2-15 B cell target epitopes. In certain embodiments, the vaccine composition comprises 2-5 B cell target epitopes. In certain embodiments, the vaccine composition comprises 5-10 B cell target epitopes. In certain embodiments, the vaccine composition comprises 5-15 B cell target epitopes. In certain embodiments, the vaccine composition comprises 5-20 B cell target epitopes. In certain embodiments, the vaccine composition comprises 5-25 B cell target epitopes. In certain embodiments, the vaccine composition comprises 5-30 B cell target epitopes. In certain embodiments, the vaccine composition comprises 10-20 B cell target epitopes. In certain embodiments, the vaccine composition comprises 10-30 B cell target epitopes.

For certain embodiments, the epitopes that are selected may be those that achieve a particular score in a binding assay (for binding to an HLA molecule, for example.) For example, in some embodiments, the epitopes selected have an IC₅₀score of 250 or less in an ELISA binding assay (e.g., an ELISA binding assay specific for HLA-DR/peptide combination, HLA-A*0201/peptide combination, etc.), or the equivalent of the IC₅₀score of 250 or less in a different binding assay. Binding assays are well known to one of ordinary skill in the art.

Large Sequence(s) Arrangements

The large sequences of the compositions described may be arranged in various configurations (see FIG. 23). In some embodiments, the large sequences may be arranged such that a spike glycoprotein (S) or a portion thereof (e.g., the RBD) is followed by an ORF1a/b protein or a portion thereof followed by Nucleoprotein or a portion thereof. In some embodiments, the large sequences may be arranged such that a spike glycoprotein (S) or a portion thereof (e.g., the RBD) is followed by an ORF1a/b protein or a portion thereof followed by Nucleoprotein, or a portion thereof is followed by a membrane (M) or a portion thereof.

In some embodiments, the large sequences may be arranged such that an ORF1a/b protein or a portion thereof followed by a nucleoprotein (N) or a portion thereof. In some embodiments, the large sequences may be arranged such that an ORF1a/b protein or a portion thereof followed by nucleoprotein (N), or a portion thereof is followed by a membrane (M) or a portion thereof.

In some embodiments, the large sequences may be arranged such that a spike glycoprotein (S) or a portion thereof (e.g., the RBD) is followed by fragment 1 or a portion thereof. In some embodiments, the large sequences may be arranged such that a spike glycoprotein (S) or a portion thereof (e.g., the RBD) is followed by fragment 2 or a portion thereof. In some embodiments, the large sequences may be arranged such that a spike glycoprotein (S) or a portion thereof (e.g., the RBD) is followed by fragment 4 or a portion thereof. In some embodiments, the large sequences may be arranged such that a spike glycoprotein (S) or a portion thereof (e.g., the RBD) is followed by fragment 5 or a portion thereof. In further embodiments, the large sequences may be arranged such that a spike glycoprotein (S) or a portion thereof (e.g., the RBD) is followed by fragment 1 or a portion thereof, followed by fragment 5 or a portion thereof.

In some embodiments, the large sequences may be arranged such that a spike glycoprotein (S) or a portion thereof (e.g., the RBD) is followed by a nucleocapsid protein or a portion thereof. In some embodiments, the large sequences may be arranged such that a spike glycoprotein (S) or a portion thereof (e.g., the RBD) is followed by a ORF1ab protein or portion thereof, followed by a ORF3 protein or portion thereof followed by an Envelope protein or protein thereof, followed by Membrane protein or portion thereof followed by an ORF6 protein or portion thereof, followed by a ORF7a protein or portion thereof. In some embodiments, the large sequences may be arranged such that a spike glycoprotein (3) or a portion thereof (e.g., the RBD) is followed by a membrane protein or portion thereof, followed by an envelope protein or portion thereof, followed by a Nsp3 protein or portion thereof, followed by a Nsp5 protein or portion thereof, followed by a Nsp12 protein or portion thereof.

In some embodiments, the large sequences may be arranged such that a spike glycoprotein (S) or a portion thereof (e.g., the RBD) is followed by one large sequence. In some embodiments, the large sequences may be arranged such that a spike glycoprotein (3) or a portion thereof (e.g., the RBD) is followed by two large sequences. In some embodiments, the large sequences may be arranged such that a spike glycoprotein (S) or a portion thereof (e.g., the RBD) is followed by three large sequences. In some embodiments, the large sequences may be arranged such that a spike glycoprotein (5) or a portion thereof (e.g., the RBD) is followed by four large sequences. In some embodiments, the large sequences may be arranged such that a spike glycoprotein (S) or a portion thereof (e.g., the RBD) is followed by five large sequences.

In some embodiments, the large sequences may be arranged such that a spike glycoprotein (S) or a portion thereof (e.g., the RBD) is followed by one large sequence both are driven each by a promoter or both are driven by a single promoter but separated by a linker as illustrated in FIG x, y and z)

Vaccine Candidates

As previously discussed, the present invention provides vaccine compositions comprising an antigen featuring: one or more large sequences, two or more large sequences, three or more large sequences, four or more large sequences, or five or more large sequences. In some embodiment, the large sequences comprise at least one B cell epitope and at least one CD4+ T cell epitope, at least one B cell epitope and at least one CD3+ T cell epitope, at least one CD4+ T cell epitope and at least one CD8+ T cell epitope, or at least one B cell epitope, at least one CD4+ T cell epitope, and at least one CD8+ T cell epitope.

Table 9 and FIG. 24 and FIG. 28 shows examples of vaccine compositions described herein. The present invention is not limited to the examples in Table 9. Per SME TRE with Cl.

TABLE 9

Vaccine

SEQ

Candidate
Sequence:
ID NO:

embedded image

CTCGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCA

TTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAA

TGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAAT

AATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGT

CAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAG

TGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATG

GCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACT

TGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGA

GCCCCACGTTCTGCTTCACTCTCCCCATCTCCCCCCCCTCCCCACCCC

CAATTTTGTATTTATTTATTTTTTAATTATTTTGTGCAGCGATGGGGGCG

GGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGGGGCGA

GGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCA

GAGCGGCGCGCTCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGC

GGCGGCCCTATAAAAAGCGAAGCGCGCGGCGGGCGGGAGTCGCTGC

GCGCTGCCTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCGCGCCGCC

CGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGG

GACGGCCCTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGG

CTTGTTTCTTTTCTGTGGCTGCGTGAAAGCCTTGAGGGGCTCCGGGAG

GGCCCTTTGTGCGGGGGGAGCGGCTCGGGGGGTGCGTGCGTGTGTG

TGTGCGTGGGGAGCGCCGCGTGCGGCTCCGCGCTGCCCGGCGGCTG

TGAGCGCTGCGGGCGCGGCGCGGGGCTTTGTGCGCTCCGCAGTGTGC

GCGAGGGGAGCGCGGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGG

139

GCTGCGAGGGGAACAAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGG

GTGAGCAGGGGGTGTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTG

CACCCCCCTCCCCGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGG

GCTCCGTACGGGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGG

TGGCGGCAGGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCC

GGGGAGGGCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGC

GGCTGTCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCG

TGCGAGAGGGCGCAGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCG

AAATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGAA

GCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTCGT

GCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGGCTG

TCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGGCGGG

GTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGCTAACCA

TGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGTGCTGGTT

ATTGTGCTGTCTCATCATTTTGGCAAAGAATTG

GAGAATAAACTAGTATT

CTTCTGGTCCCCACAGACTCAGAGAGAACCCGCCACCATGTTCGTGTT

CCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGTGAACCTGACCA

CCAGGACCCAGCTGCCCCCCGCCTACACCAACAGCTTCACCAGGGGC

GTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCTGCACAGCACC

CAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTGGTTCCACGCC

ATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGACAACCCCGT

GCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGAGAAGAGCAA

CATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAGCAAGACCCA

GAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGATCAAGGTGTG

CGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTACTACCACAA

GAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTACAGCAGCG

CCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCTGATGGACC

TGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGTTCGTGTTCA

AGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACACCCCCATCAA

CCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCCTGGAGCCCCTGG

TGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGACCCTGCTGG

CCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGCAGCGGCTGG

ACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCAGCCCAGGAC

CTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGACGCCGTGGA

CTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGCACCCTGAAGAGCTT

CACCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCAGGGTGCAGCC

CACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACCTGTGCCCCTT

CGGCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGTACGCCTGGA

ACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTACA

ACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTGAGCCCCACCA

AGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTCGTGA

TCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCAGACCGGCAA

GATCGCCGACTACAACTACAAGCTGCCCGACGACTTCACCGGCTGCGT

GATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGGGCGGCAACT

ACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGAAGCCCTTCG

AGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAGCACCCCCTGC

AACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTGCAGAGCTACGGC

TTCCAGCCCACCAACGGCGTGGGCTACCAGCCCTACAGGGTGGTGGT

GCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGTGCGGCCCCA

AGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAA

CGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACAAGAAGTTCC

TGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCACCGACGCC

GTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACCCCCTGCAG

CTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACACCAGCAACC

AGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGGTGCCCGTG

GCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTGTACAGCAC

CGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGATCGGCGCCG

AGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCGGCGCCGGC

ATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCGGCAGCGCCAG

C
AGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAGCCTGGGCG

CCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCATCCCCACCA

ACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGAGCATGACCA

AGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACAGCACCGAGT

GCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCACCCAGCTGAACA

GGGCCCTGACCGGCATCGCCGTGGAGCAGGACAAGAACACCCAGGAG

GTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCCCCCATCAAGGAC

TTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCGACCCCAGCAAGCCC

AGCAAGAGGAGCCCCATCGAGGACCTGCTGTTCAACAAGGTGACCCTG

GCCGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCTGGGCGACAT

CGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACGGCCTGACCG

TGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAGTACACCAGCG

CCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTTCGGCGCCGGC

CCC
GCCCTGCAGATCCCCTTCCCCATGCAGATGGCCTACAGGTTCAAC

GGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACCAGAAGCTGATC

GCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGGACAGCCTGAGC

AGCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGTGGTGAACCAGAA

CGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAGCAGCAACTTCG

GCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAGGCTGGACCCC

CCC
GAGGCCGAGGTGCAGATCGACAGGCTGATCACCGGCAGGCTGCA

GAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGGGCCGCCGAGA

TCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAGCGAGTGCGTG

CTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGGGCTACCACCT

GATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTGTTCCTGCACG

TGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACCGCCCCCGCCA

TCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGGCGTGTTCGTG

AGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTTCTACGAGCCC

CAGATCATCACCACCGACAACACCTTCGTGAGCGGCAACTGCGACGTG

GTGATCGGCATCGTGAACAACACCGTGTACGACCCCCTGCAGCCCGAG

CTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAAGAACCACACC

AGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCAACGCCAGCGT

GGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAGGTGGCCAAGA

ACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGGCAAGTACGAG

CAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTTCATCGCCGGC

CTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTGCATGACCAGC

TGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGGCAGCTGCTGCAA

GTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGCGTGAAGCTGC

ACTACACCGGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAG

GAGATGTTGAAGAAAACCCCGGGCCT
ATGAGCGACAACGGCCCCCAG

AACCAGAGGAACGCCCCCAGGATCACCTTCGGCGGCCCCAGCGACA

GCACCGGCAGCAACCAGAACGGCGAGAGGAGCGGCGCCAGGAGCA

AGCAGAGGAGGCCCCAGGGCCTGCCCAACAACACCGCCAGCTGGTT

CACCGCCCTGACCCAGCACGGCAAGGAGGACCTGAAGTTCCCCAGG

GGCCAGGGCGTGCCCATCAACACCAACAGCAGCCCCGACGACCAGA

TCGGCTACTACAGGAGGGCCACCAGGAGGATCAGGGGCGGCGACGG

CAAGATGAAGGACCTGAGCCCCAGGTGGTACTTCTACTACCTGGGCA

CCGGCCCCGAGGCCGGCCTGCCCTACGGCGCCAACAAGGACGGCAT

CATCTGGGTGGCCACCGAGGGCGCCCTGAACACCCCCAAGGACCAC

ATCGGCACCAGGAACCCCGCCAACAACGCCGCCATCGTGCTGCAGCT

GCCCCAGGGCACCACCCTGCCCAAGGGCTTCTACGCCGAGGGCAGC

AGGGGCGGCAGCCAGGCCAGCAGCAGGAGCAGCAGCAGGAGCAGG

AACAGCAGCAGGAACAGCACCCCCGGCAGCAGCAGGGGCACCAGCC

CCGCCAGGATGGCCGGCAACGGCGGCGACGCCGCCCTGGCCCTGCT

GCTGCTGGACAGGCTGAACCAGCTGGAGAGCAAGATGAGCGGCAAG

GGCCAGCAGCAGCAGGGCCAGACCGTGACCAAGAAGAGCGCCGCCG

AGGCCAGCAAGAAGCCCAGGCAGAAGAGGACCGCCACCAAGGCCTA

CAACGTGACCCAGGCCTTCGGCAGGAGGGGCCCCGAGCAGACCCAG

GGCAACTTCGGCGACCAGGAGCTGATCAGGCAGGGCACCGACTACA

AGCACTGGCCCCAGATCGCCCAGTTCGCCCCCAGCGCCAGCGCCTTC

TTCGGCATGAGCAGGATCGGCATGGAGGTGACCCCCAGCGGCACCT

GGCTGACCTACACCGGCGCCATCAAGCTGGACGACAAGGACCCCAA

CTTCAAGGACCAGGTGATCCTGCTGAACAAGCACATCGACGCCTACA

AGACCTTCCCCCCCACCGAGCCCAAGAAGGACAAGAAGAAGAAGGC

CGACGAGACCCAGGCCCTGCCCCAGAGGCAGAAGAAGCAGCAGACC

GTGACCCTGCTGCCCGCCGCCGACCTGGACGACTTCAGCAAGCAGCT

embedded image

CTCGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCA

TTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAA

TGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAAT

AATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGT

CAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAG

TGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATG

GCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACT

TGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGA

GCCCCACGTTCTGCTTCACTCTCCCCATCTCCCCCCCCTCCCCACCCC

CAATTTTGTATTTATTTATTTTTTAATTATTTTGTGCAGCGATGGGGGCG

GGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGGGGCGA

GGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCA

GAGCGGCGCGCTCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGC

GGCGGGCCTATAAAAAGCGAAGCGCGCGGCGGGCGGGAGTCGCTGC

GCGCTGCCTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCGCGCCGCC

CGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGG

GACGGCCCTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGG

CTTGTTTCTTTTCTGTGGCTGCGTGAAAGCCTTGAGGGGCTCCGGGAG

GGCCCTTTGTGCGGGGGGAGCGGCTCGGGGGGTGCGTGCGTGTGTG

TGTGCGTGGGGAGCGCCGCGTGCGGCTCCGCGCTGCCCGGCGGCTG

TGAGCGCTGCGGGCGCGGCGCGGGGCTTTGTGCGCTCCGCAGTGTGC

GCGAGGGGAGCGCGGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGG

GCTGCGAGGGGAACAAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGG

GTGAGCAGGGGGTGTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTG

CACCCCCCTCCCCGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGG

GCTCCGTACGGGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGG

140

TGGCGGCAGGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCC

GGGGAGGGCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGC

GGCTGTCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCG

TGCGAGAGGGCGCAGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCG

AAATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGAA

GCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTCGT

GCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGGCTG

TCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGGCGGG

GTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGCTAACCA

TGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGTGCTGGTT

ATTGTGCTGTCTCATCATTTTGGCAAAGAATTG

GAGAATAAACTAGTATT

CTTCTGGTCCCCACAGACTCAGAGAGAACCCGCCACCATGTTCGTGTT

CCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGTGAACCTGACCA

CCAGGACCCAGCTGCCCCCCGCCTACACCAACAGCTTCACCAGGGGC

GTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCTGCACAGCACC

CAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTGGTTCCACGCC

ATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGACAACCCCGT

GCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGAGAAGAGCAA

CATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAGCAAGACCCA

GAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGATCAAGGTGTG

CGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTACTACCACAA

GAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTACAGCAGCG

CCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCTGATGGACC

TGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGTTCGTGTTCA

AGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACACCCCCATCAA

CCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCCTGGAGCCCCTGG

TGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGACCCTGCTGG

CCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGCAGCGGCTGG

ACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCAGCCCAGGAC

CTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGACGCCGTGGA

CTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGCACCCTGAAGAGCTT

CACCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCAGGGTGCAGCC

CACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACCTGTGCCCCTT

CGGCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGTACGCCTGGA

ACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTACA

ACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTGAGCCCCACCA

AGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTCGTGA

TCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCAGACCGGCAA

GATCGCCGACTACAACTACAAGCTGCCCGACGACTTCACCGGCTGCGT

GATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGGGCGGCAACT

ACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGAAGCCCTTCG

AGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAGCACCCCCTGC

AACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTGCAGAGCTACGGC

TTCCAGCCCACCAACGGCGTGGGCTACCAGCCCTACAGGGTGGTGGT

GCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGTGCGGCCCCA

AGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAA

CGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACAAGAAGTTCC

TGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCACCGACGCC

GTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACCCCCTGCAG

CTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACACCAGCAACC

AGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGGTGCCCGTG

GCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTGTACAGCAC

CGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGATCGGCGCCG

AGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCGGCGCCGGC

ATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCGGCAGCGCCAG

C
AGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAGCCTGGGCG

CCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCATCCCCACCA

ACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGAGCATGACCA

AGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACAGCACCGAGT

GCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCACCCAGCTGAACA

GGGCCCTGACCGGCATCGCCGTGGAGCAGGACAAGAACACCCAGGAG

GTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCCCCCATCAAGGAC

TTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCGACCCCAGCAAGCCC

AGCAAGAGGAGCCCCATCGAGGACCTGCTGTTCAACAAGGTGACCCTG

GCCGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCTGGGCGACAT

CGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACGGCCTGACCG

TGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAGTACACCAGCG

CCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTTCGGCGCCGGC

CCC
GCCCTGCAGATCCCCTTCCCCATGCAGATGGCCTACAGGTTCAAC

GGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACCAGAAGCTGATC

GCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGGACAGCCTGAGC

AGCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGTGGTGAACCAGAA

CGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAGCAGCAACTTCG

GCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAGGCTGGACCCC

CCC
GAGGCCGAGGTGCAGATCGACAGGCTGATCACCGGCAGGCTGCA

GAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGGGCCGCCGAGA

TCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAGCGAGTGCGTG

CTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGGGCTACCACCT

GATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTGTTCCTGCACG

TGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACCGCCCCCGCCA

TCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGGCGTGTTCGTG

AGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTTCTACGAGCCC

CAGATCATCACCACCGACAACACCTTCGTGAGCGGCAACTGCGACGTG

GTGATCGGCATCGTGAACAACACCGTGTACGACCCCCTGCAGCCCGAG

CTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAAGAACCACACC

AGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCAACGCCAGCGT

GGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAGGTGGCCAAGA

ACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGGCAAGTACGAG

CAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTTCATCGCCGGC

CTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTGCATGACCAGC

TGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGGCAGCTGCTGCAA

GTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGCGTGAAGCTGC

ACTACACCGGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAG

GAGATGTTGAAGAAAACCCCGGGCCT
CAAACCACTGAAACAGCWCAC

TCTTGTAATGTTAACCGCTTTAATGTGGCTATTACAAGAGCAAAAATTG

GCATTTTGTGCATAATGTCTGACAGAGATCTTTATGACAAGCTGCAATT

CACAAGTCTAGAAGTACCGCGTCGTAACGTGGCTACATTACAAGCGG

AAAATGTAACTGGACTCTTTAAGGACTGTAGTAAGATCATAACTGGTC

TTCATCCTACACAAGCACCTACACACCTTAGTGTTGATACAAAATTCA

AGACTGAGGGACTATGTGTTGACATACCAGGCATWCCWAAGGACAT

GACCTATMGWAGACTCATCTCYATGATGGGTTTCAAAATGAATTAYCA

AGTTAATGGTTACCCTAAYATGTTYATCACCCGYGARGAAGCCATMMG

MCAYGTWCGTGCATGGATTGGCTTTGATGTAGAGGGKTGTCATGCTA

CTAGGGATGCTGTCGGTACTAACCTACCTCTCCAGTTAGGATTTTCTA

CAGGTGTTAACTTAGTAGCTGTACCAACTGGCTATGTTGACACTGAAA

ACAATACAGAATTCACCAGAGTTAATGCAAAACCTCCACCAGGTGAC

CAATTTAAACATCTTATACCACTTATGTACAAAGGTTTACCCTGGAACA

TAGTGCGTATCAAGATAGTACAAATGCTCAGTGATACACTGAAAGGAT

TATCRGACAGAGTTGTGTTTGTCCTATGGGCACATGGCTTTGAACTTA

CATCAATGAAGTACTTTGTCAAGATTGGACCTGAAAGAACGTGTTGTC

TGTGTGACAAACGTGCAACTTGTTTTTCTACTTCATCAGACAATTATGC

CTGCTGGAACCATTCTGTGGGTTTTGACTATGTCTATAATCCATTTATG

ATTGATGTCCAGCAGTGGGGTTTTACAGGTAACCTTCAGAGTAATCAC

GATCAGCATTGCCAAGTGCATGGCAACGCTCATGTGGCTAGTTGTGAT

GCTATCATGACTAGATGTTTAGCAGTCCATGAGTGCTTTGTTAAGCGC

GTTGACTGGTCTGTTGAGTACCCAATTATAGGTGATGAACTGAAGATC

AATGCCGCATGCAGAAAAGTGCAACATATGGTTGTAAAGTCTGCATTG

CTTGCTGACAAATTCCCAGTTCTTCATGACATTGGAAACCCAAAGGCT

ATCAAATGTGTCCCRCAGGCTGAAGTGGATTGGAAGTTCTATGATGCT

CAGCCCTGCAGTGACAAAGCTTATAAAATAAAAGAACTCTTCTATTCT

TATGCTACACATCATGATAAATTCATTGATGGTGTTTGTTTATTTTGGA

ATTGTAACGTTGATCGTTACCCTGCCAATGCTATTGTRTGCAGGTTCG

ACACGAGAGTCTTGTCAAATTTGAACTTGCCAGGTTGTGATGGTGGTA

GTTTGTATGTAAATAAGCATGCATTCCACACTCCAGCTTTTGATAAAAG

TGCATTTACTAATTTAAAGCAATTGCCTTTCTTTTATTACTCTGACAGTC

CCTGTGAGTCACATGGCAAGCAGGTTGTTTCTGACATTGATTATGTAC

CACTCAAATCTGCTACRTGTATAACACGATGCAATTTGGGRGGTGCTG

TTTGCAGACATCATGCAAATGAGTACCGACAGTACTTGGATGCATACA

ATATGATGATTTCTGCTGGCTTTAGCCTCTGGATTTACAAACAGTTTGA

CACTTATAACCTGTGGAACACCTTTACCAGGTTACAGAGTTTAGAAAA

TGTGGCTTACAATGTTGTTAACAAAGGACACTTCGATGGACAAGCTGG

TGAAGCACCTGTTTCCGTCATTAATAATGTTGTTTACACAAAGGTAGAT

GGTGTTGATGTAGAGATCTTTGAAAACAAGACAACACTTCCTGTTAAT

GTTGCATTTGAGCTTTGGGCTAAGCGTAACATTAAACCAGTGCCAGAG

ATTAAGATACTCAATAATTTGGGTGTCGATATCGCTGCTAATACTGTAA

TCTGGGACTACAAGAGAGAAGCACCAGCACATATGTCAACAATAGGT

GTCTGCACAATGACTGACATTGCCAAGAAACCTACTGAGAGTGCTTGT

TCCTCGCTTACTGTCTTATTTGATGGTAGAGTGGAAGGACAGGTAGAC

CTTTTTAGAAATGCCCGTAATGGTGTTTTAATAACAGAAGGTTCAGTTA

AAGGTTTAATACCTTCAAAGGGACCAGCACAAGCTAGTGTCAATGGA

GTCACATTAATTGGAGAATCAGTAAAAACACAGTTTAATTATTTTAAGA

AAGTAGATGGCATCATTCAACAGTTGCCTGAAACCTACTTTACTCAGA

GCCGAGACTTAGAGGATTTCAAGCCCAGATCACAAATGGAAACTGAC

TTTCTTGAGCTCGCTATGGATGAATTCATACAACGGTACAAGCTTGAA

GGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATGGACAG

CTTGGTGGACTTCATCTAATGATTGGTCTAGCTAAGCGCTCACAAGAT

TCACCACTTAAATTAGAGGATTTTATCCCTACGGACAGTACAGTGAAA

AATTATTTCATAACAGATGCGCAAACAGGTTCATCAAAATGCGTGTGC

TCTGTTATTGATCTTCTGCTTGATGACTTTGTTGAGATAATAAAGTCAC

AAGATTTATCAGTGGTTTCAAAGGTGGTCAAAGTCACAATTGACTATG

CTGAAATTTCATTCATGTTATGGTGTAAGGATGGACATGTTGAAACCTT

TTACCCAAAATTACAAGCGAGTCAGGCGTGGCAACCAGGAGTTGCAA

TGCCTAACTTGTATAAGATGCAGAGAATGCTTCTTGAAAAATGTGACC

TTCAGAATTATGGTGAAAATGCTGTCATACCAAARGGAATAATGATGA

ATGTCGCAAAATATACTCAACTGTGTCAATATTTAAATACACTYACATT

AGCYGTGCCATATAATATGAGAGTTATCCATTTTGGTGCTGGCTCRGA

CAAAGGAGTTGCACCCGGCACAGCTGTTCTCAGACAGTGGTTGCCAA

TTGGCACACTACTTGTTGATTCAGATCTTAACGACTTCGTCTCTGACGC

TGATTCCACTCTAATTGGAGACTGTGCAACCGTACATACAGCTAACAA

ATGGGATCTCATTATTAGCGATATGTATGATCCTAAAACCAAACACGT

GACAAAGGAAAATGATTCAAAAGAAGGATTTTTCACTTACCTGTGTGG

ATTTATTAAACAAAAATTAGCCCTGGGAGGCTCTGTGGCTGTAAAGAT

AACTGAGCATTCTTGGAATGCGGATCTCTACAAGCTCATGGGACATTT

CTCATGGTGGACAGCTTTTGTTACAAATGTTAATGCATCTTCATCAGAA

GCATTTTTAATTGGAGTTAACTATCTTGGTAAGCCAAAAGAACAAATT

GATGGTTACACCATGCATGCTAACTACATTTTCTGGAGGAATACAAAC

CCGATTCAATTGTCTTCCTATTCACTTTTTGACATGAGTAAGTTCCCTC

TTAAATTAAGGGGAACAGCTGTCATGTCTTTAAAGGAGAACCAAATCA

ATGAAATGATTTATTCTCTACTTGAAAAAGGCAGACTTATCATTAGGG

AAAACAACAGAGTTGTTGTCTCAAGTGATGTTCTTGTTAATAACTAAAC

embedded image

AACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGC

CT
ATGAAGATCATCCTGTTCCTGGCCCTGATCACCCTGGCCACCTGCG

embedded image

AGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAAAAC

embedded image

GGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAAA

embedded image

10
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA
295

Ad5-Pan-CoV
TGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGAC

Spike-NSP1-
CGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT

NSP2
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA

CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTA

CGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATG

CCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT

ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAAT

GGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCC

ATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTC

CAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGC

GTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTC

AGATCGAGAATAAACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGA

ACCCGCCACCATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAG

CCAGTGCGTGAACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACA

CCAACAGCTTCACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGA

GCAGCGTGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCA

ACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCA

AGAGGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCG

CCAGCACCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCA

CCCTGGACAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCA

ACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCC

TGGGCGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGT

TCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCC

AGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACC

TGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAG

CAAGCACACCCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAG

CGCCCTGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAG

GTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCG

ACAGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGC

TACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACC

ATCACCGACGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAA

GTGCACCCTGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAG

CAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACAT

CACCAACCTGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGC

CAGCGTGTACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCG

ACTACAGCGTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCT

ACGGCGTGAGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGT

ACGCCGACAGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCC

CCCGGCCAGACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGA

CGACTTCACCGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAG

CAAGGTGGGCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAG

CAACCTGAAGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGC

CGGCAGCACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCC

CCTGCAGAGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGC

CCTACAGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCC

ACCGTGTGCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGC

GTGAACTTCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAG

AGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCC

GACACCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGA

CATCACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCA

CCAACACCAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCA

CCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGG

AGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTG

CCTGATCGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCC

CATCGGCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCC

CCAGGAGGGCCAGGAGCGTGGCCAGCCAGAGCATCATCGCCTACACC

ATGAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCAT

CGCCATCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCC

CGTGAGCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGG

CGACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTG

CACCCAGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACA

AGAACACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCC

CCCCCATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCG

ACCCCAGCAAGCCCAGCAAGAGGAGCTTCATCGAGGACCTGCTGTTCA

ACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACT

GCCTGGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTC

AACGGCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGC

CCAGTACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGA

CCTTCGGCGCCGGCGCCGCCCTGCAGATCCCCTTCGCCATGCAGATG

GCCTACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAG

AACCAGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATC

CAGGACAGCCTGAGCAGCACCGCCAGCGCCCTGGGCAAGCTGCAGGA

CGTGGTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGC

TGAGCAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGA

GCAGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATC

ACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGAT

CAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGA

TGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGC

AAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGT

GGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCAC

CACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGG

AGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGG

AACTTCTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGC

GGCAACTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGAC

CCCCTGCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTA

CTTCAAGAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCG

GCATCAACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGA

ACGAGGTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAG

CTGGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTG

GGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTG

TGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGC

GGCAGCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAA

GGGCGTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCT

GTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTATGGAGAG

CCTGGTGCCCGGCTTCAACGAGAAGACCCACGTGCAGCTGAGCCTGC

CCGTGCTGCAGGTGAGGGACGTGCTGGTGAGGGGCTTCGGCGACAGC

GTGGAGGAGGTGCTGAGCGAGGCCAGGCAGCACCTGAAGGACGGCAC

CTGCGGCCTGGTGGAGGTGGAGAAGGGCGTGCTGCCCCAGCTGGAGC

AGCCCTACGTGTTCATCAAGAGGAGCGACGCCAGGACCGCCCCCCAC

GGCCACGTGATGGTGGAGCTGGTGGCCGAGCTGGAGGGCATCCAGTA

CGGCAGGAGCGGCGAGACCCTGGGCGTGCTGGTGCCCCACGTGGGC

GAGATCCCCGTGGCCTACAGGAAGGTGCTGCTGAGGAAGAACGGCAA

CAAGGGCGCCGGCGGCCACAGCTACGGCGCCGACCTGAAGAGCTTCG

ACCTGGGCGACGAGCTGGGCACCGACCCCTACGAGGACTTCCAGGAG

AACTGGAACACCAAGCACAGCAGCGGCGTGACCAGGGAGCTGATGAG

GGAGCTGAACGGCGGCGGAAGCGGAGAGGGCAGGGGAAGTCTTCTAA

CATGCGGGGACGTGGAGGAAAATCCCGGCCCCGCCTACACCAGGTAC

GTGGACAACAACTTCTGCGGCCCCGACGGCTACCCCCTGGAGTGCATC

AAGGACCTGCTGGCCAGGGCCGGCAAGGCCAGCTGCACCCTGAGCGA

GCAGCTGGACTTCATCGACACCAAGAGGGGCGTGTACTGCTGCAGGG

AGCACGAGCACGAGATCGCCTGGTACACCGAGAGGAGCGAGAAGAGC

TACGAGCTGCAGACCCCCTTCGAGATCAAGCTGGCCAAGAAGTTCGAC

ACCTTCAACGGCGAGTGCCCCAACTTCGTGTTCCCCCTGAACAGCATC

ATCAAGACCATCCAGCCCAGGGTGGAGAAGAAGAAGCTGGACGGCTTC

ATGGGCAGGATCAGGAGCGTGTACCCCGTGGCCAGCCCCAACGAGTG

CAACCAGATGTGCCTGAGCACCCTGATGAAGTGCGACCACTGCGGCGA

GACCAGCTGGCAGACCGGCGACTTCGTGAAGGCCACCTGCGAGTTCT

GCGGCACCGAGAACCTGACCAAGGAGGGCGCCACCACCTGCGGCTAC

CTGCCCCAGAACGCCGTGGTGAAGATCTACTGCCCCGCCTGCCACAAC

AGCGAGGTGGGCCCCGAGCACAGCCTGGCCGAGTACCACAACGAGAG

CGGCCTGAAGACCATCCTGAGGAAGGGCGGCAGGACCATCGCCTTCG

GCGGCTGCGTGTTCAGCTACGTGGGCTGCCACAACAAGTGCGCCTACT

GGGTGCCCAGGGCCAGCGCCAACATCGGCTGCAACCACACCGGCGTG

GTGGGCGAGGGCAGCGAGGGCCTGAACGACAACCTGCTGGAGATCCT

GCAGAAGGAGAAGGTGAACATCAACATCGTGGGCGACTTCAAGCTGAA

CGAGGAGATCGCCATCATCCTGGCCAGCTTCAGCGCCAGCACCAGCG

CCTTCGTGGAGACCGTGAAGGGCCTGGACTACAAGGCCTTCAAGCAGA

TCGTGGAGAGCTGCGGCAACTTCAAGGTGACCAAGGGCAAGGCCAAG

AAGGGCGCCTGGAACATCGGCGAGCAGAAGAGCATCCTGAGCCCCCT

GTACGCCTTCGCCAGCGAGGCCGCCAGGGTGGTGAGGAGCATCTTCA

GCAGGACCCTGGAGACCGCCCAGAACAGCGTGAGGGTGCTGCAGAAG

GCCGCCATCACCATCCTGGACGGCATCAGCCAGTACAGCCTGAGGCTG

ATCGACGCCATGATGTTCACCAGCGACCTGGCCACCAACAACCTGGTG

GTGATGGCCTACATCACCGGCGGCGTGGTGCAGCTGACCAGCCAGTG

GCTGACCAACATCTTCGGCACCGTGTACGAGAAGCTGAAGCCCGTGCT

GGACTGGCTGGAGGAGAAGTTCAAGGAGGGCGTGGAGTTCCTGAGGG

ACGGCTGGGAGATCGTGAAGTTCATCAGCACCTGCGCCTGCGAGATCG

TGGGCGGCCAGATCGTGACCTGCGCCAAGGAGATCAAGGAGAGCGTG

CAGACCTTCTTCAAGCTGGTGAACAAGTTCCTGGCCCTGTGCGCCGAC

AGCATCATCATCGGCGGCGCCAAGCTGAAGGCCCTGAACCTGGGCGA

GACCTTCGTGACCCACAGCAAGGGCCTGTACAGGAAGTGCGTGAAGAG

CAGGGAGGAGACCGGCCTGCTGATGCCCCTGAAGGCCCCCAAGGAGA

TCATCTTCCTGGAGGGCGAGACCCTGCCCACCGAGGTGCTGACCGAG

GAGGTGGTGCTGAAGACCGGCGACCTGCAGCCCCTGGAGCAGCCCAC

CAGCGAGGCCGTGGAGGCCCCCCTGGTGGGCACCCCCGTGTGCATCA

ACGGCCTGATGCTGCTGGAGATCAAGGACACCGAGAAGTACTGCGCCC

TGGCCCCCAACATGATGGTGACCAACAACACCTTCACCCTGAAGGGCG

GCTGATGACTCGAGCTGGTACTGCATGCACGCAATGCTAGCTGCCCCT

TTCCCGTCCTGGGTACCCCGAGTCTCCCCCGACCTCGGGTCCCAGGTA

TGCTCCCACCTCCACCTGCCCCACTCACCACCTCTGCTAGTTCCAGACA

CCTCCCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACA

CCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTT

AACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACAC

CCTGGAGCTAGCAAAAAAAA

11
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA
296

Ad5-Pan-CoV
TGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGAC

HexaPro-
CGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT

NSP1-NSP2
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA

CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTA

CGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATG

CCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT

ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAAT

GGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCC

ATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTC

CAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGC

GTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTC

AGATCGAGAATAAACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGA

ACCCGCCACCATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAG

CCAGTGCGTGAACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACA

CCAACAGCTTCACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGA

GCAGCGTGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCA

ACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCA

AGAGGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCG

CCAGCACCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCA

CCCTGGACAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCA

ACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCC

TGGGCGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGT

TCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCC

AGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACC

TGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAG

CAAGCACACCCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAG

CGCCCTGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAG

GTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCG

ACAGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGC

TACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACC

ATCACCGACGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAA

GTGCACCCTGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAG

CAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACAT

CACCAACCTGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGC

CAGCGTGTACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCG

ACTACAGCGTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCT

ACGGCGTGAGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGT

ACGCCGACAGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCC

CCCGGCCAGACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGA

CGACTTCACCGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAG

CAAGGTGGGCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAG

CAACCTGAAGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGC

CGGCAGCACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCC

CCTGCAGAGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGC

CCTACAGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCC

ACCGTGTGCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGC

GTGAACTTCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAG

AGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCC

GACACCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGA

CATCACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCA

CCAACACCAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCA

CCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGG

AGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTG

CCTGATCGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCC

CATCGGCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCC

CCGGCAGCGCCAGCAGCGTGGCCAGCCAGAGCATCATCGCCTACACC

ATGAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCAT

CGCCATCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCC

CGTGAGCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGG

CGACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTG

CACCCAGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACA

AGAACACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCC

CCCCCATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCG

ACCCCAGCAAGCCCAGCAAGAGGAGCCCCATCGAGGACCTGCTGTTCA

ACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACT

GCCTGGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTC

AACGGCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGC

CCAGTACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGA

CCTTCGGCGCCGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATG

GCCTACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAG

AACCAGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATC

CAGGACAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGA

CGTGGTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGC

TGAGCAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGA

GCAGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATC

ACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGAT

CAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGA

TGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGC

AAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGT

GGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCAC

CACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGG

AGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGG

AACTTCTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGC

GGCAACTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGAC

CCCCTGCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTA

CTTCAAGAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCG

GCATCAACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGA

ACGAGGTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAG

CTGGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTG

GGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTG

TGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGC

GGCAGCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAA

GGGCGTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCT

GTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTATGGAGAG

CCTGGTGCCCGGCTTCAACGAGAAGACCCACGTGCAGCTGAGCCTGC

CCGTGCTGCAGGTGAGGGACGTGCTGGTGAGGGGCTTCGGCGACAGC

GTGGAGGAGGTGCTGAGCGAGGCCAGGCAGCACCTGAAGGACGGCAC

CTGCGGCCTGGTGGAGGTGGAGAAGGGCGTGCTGCCCCAGCTGGAGC

AGCCCTACGTGTTCATCAAGAGGAGCGACGCCAGGACCGCCCCCCAC

GGCCACGTGATGGTGGAGCTGGTGGCCGAGCTGGAGGGCATCCAGTA

CGGCAGGAGCGGCGAGACCCTGGGCGTGCTGGTGCCCCACGTGGGC

GAGATCCCCGTGGCCTACAGGAAGGTGCTGCTGAGGAAGAACGGCAA

CAAGGGCGCCGGCGGCCACAGCTACGGCGCCGACCTGAAGAGCTTCG

ACCTGGGCGACGAGCTGGGCACCGACCCCTACGAGGACTTCCAGGAG

AACTGGAACACCAAGCACAGCAGCGGCGTGACCAGGGAGCTGATGAG

GGAGCTGAACGGCGGCGGAAGCGGAGAGGGCAGGGGAAGTCTTCTAA

CATGCGGGGACGTGGAGGAAAATCCCGGCCCCGCCTACACCAGGTAC

GTGGACAACAACTTCTGCGGCCCCGACGGCTACCCCCTGGAGTGCATC

AAGGACCTGCTGGCCAGGGCCGGCAAGGCCAGCTGCACCCTGAGCGA

GCAGCTGGACTTCATCGACACCAAGAGGGGCGTGTACTGCTGCAGGG

AGCACGAGCACGAGATCGCCTGGTACACCGAGAGGAGCGAGAAGAGC

TACGAGCTGCAGACCCCCTTCGAGATCAAGCTGGCCAAGAAGTTCGAC

ACCTTCAACGGCGAGTGCCCCAACTTCGTGTTCCCCCTGAACAGCATC

ATCAAGACCATCCAGCCCAGGGTGGAGAAGAAGAAGCTGGACGGCTTC

ATGGGCAGGATCAGGAGCGTGTACCCCGTGGCCAGCCCCAACGAGTG

CAACCAGATGTGCCTGAGCACCCTGATGAAGTGCGACCACTGCGGCGA

GACCAGCTGGCAGACCGGCGACTTCGTGAAGGCCACCTGCGAGTTCT

GCGGCACCGAGAACCTGACCAAGGAGGGCGCCACCACCTGCGGCTAC

CTGCCCCAGAACGCCGTGGTGAAGATCTACTGCCCCGCCTGCCACAAC

AGCGAGGTGGGCCCCGAGCACAGCCTGGCCGAGTACCACAACGAGAG

CGGCCTGAAGACCATCCTGAGGAAGGGCGGCAGGACCATCGCCTTCG

GCGGCTGCGTGTTCAGCTACGTGGGCTGCCACAACAAGTGCGCCTACT

GGGTGCCCAGGGCCAGCGCCAACATCGGCTGCAACCACACCGGCGTG

GTGGGCGAGGGCAGCGAGGGCCTGAACGACAACCTGCTGGAGATCCT

GCAGAAGGAGAAGGTGAACATCAACATCGTGGGCGACTTCAAGCTGAA

CGAGGAGATCGCCATCATCCTGGCCAGCTTCAGCGCCAGCACCAGCG

CCTTCGTGGAGACCGTGAAGGGCCTGGACTACAAGGCCTTCAAGCAGA

TCGTGGAGAGCTGCGGCAACTTCAAGGTGACCAAGGGCAAGGCCAAG

AAGGGCGCCTGGAACATCGGCGAGCAGAAGAGCATCCTGAGCCCCCT

GTACGCCTTCGCCAGCGAGGCCGCCAGGGTGGTGAGGAGCATCTTCA

GCAGGACCCTGGAGACCGCCCAGAACAGCGTGAGGGTGCTGCAGAAG

GCCGCCATCACCATCCTGGACGGCATCAGCCAGTACAGCCTGAGGCTG

ATCGACGCCATGATGTTCACCAGCGACCTGGCCACCAACAACCTGGTG

GTGATGGCCTACATCACCGGCGGCGTGGTGCAGCTGACCAGCCAGTG

GCTGACCAACATCTTCGGCACCGTGTACGAGAAGCTGAAGCCCGTGCT

GGACTGGCTGGAGGAGAAGTTCAAGGAGGGCGTGGAGTTCCTGAGGG

ACGGCTGGGAGATCGTGAAGTTCATCAGCACCTGCGCCTGCGAGATCG

TGGGCGGCCAGATCGTGACCTGCGCCAAGGAGATCAAGGAGAGCGTG

CAGACCTTCTTCAAGCTGGTGAACAAGTTCCTGGCCCTGTGCGCCGAC

AGCATCATCATCGGCGGCGCCAAGCTGAAGGCCCTGAACCTGGGCGA

GACCTTCGTGACCCACAGCAAGGGCCTGTACAGGAAGTGCGTGAAGAG

CAGGGAGGAGACCGGCCTGCTGATGCCCCTGAAGGCCCCCAAGGAGA

TCATCTTCCTGGAGGGCGAGACCCTGCCCACCGAGGTGCTGACCGAG

GAGGTGGTGCTGAAGACCGGCGACCTGCAGCCCCTGGAGCAGCCCAC

CAGCGAGGCCGTGGAGGCCCCCCTGGTGGGCACCCCCGTGTGCATCA

ACGGCCTGATGCTGCTGGAGATCAAGGACACCGAGAAGTACTGCGCCC

TGGCCCCCAACATGATGGTGACCAACAACACCTTCACCCTGAAGGGCG

GCTGATGACTCGAGCTGGTACTGCATGCACGCAATGCTAGCTGCCCCT

TTCCCGTCCTGGGTACCCCGAGTCTCCCCCGACCTCGGGTCCCAGGTA

TGCTCCCACCTCCACCTGCCCCACTCACCACCTCTGCTAGTTCCAGACA

CCTCCCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACA

CCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTT

AACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACAC

CCTGGAGCTAGCAAAAAAAA

12
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA
237

Ad5-Pan-CoV
TGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGAC

Spike-NSP6-
CGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT

NSP7-NSP8
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA

CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTA

CGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATG

CCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT

ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAAT

GGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCC

ATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTC

CAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGC

GTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTC

AGATCGAGAATAAACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGA

ACCCGCCACCATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAG

CCAGTGCGTGAACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACA

CCAACAGCTTCACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGA

GCAGCGTGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCA

ACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCA

AGAGGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCG

CCAGCACCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCA

CCCTGGACAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCA

ACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCC

TGGGCGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGT

TCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCC

AGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACC

TGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAG

CAAGCACACCCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAG

CGCCCTGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAG

GTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCG

ACAGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGC

TACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACC

ATCACCGACGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAA

GTGCACCCTGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAG

CAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACAT

CACCAACCTGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGC

CAGCGTGTACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCG

ACTACAGCGTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCT

ACGGCGTGAGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGT

ACGCCGACAGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCC

CCCGGCCAGACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGA

CGACTTCACCGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAG

CAAGGTGGGCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAG

CAACCTGAAGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGC

CGGCAGCACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCC

CCTGCAGAGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGC

CCTACAGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCC

ACCGTGTGCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGC

GTGAACTTCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAG

AGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCC

GACACCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGA

CATCACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCA

CCAACACCAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCA

CCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGG

AGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTG

CCTGATCGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCC

CATCGGCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCC

CCAGGAGGGCCAGGAGCGTGGCCAGCCAGAGCATCATCGCCTACACC

ATGAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCAT

CGCCATCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCC

CGTGAGCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGG

CGACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTG

CACCCAGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACA

AGAACACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCC

CCCCCATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCG

ACCCCAGCAAGCCCAGCAAGAGGAGCTTCATCGAGGACCTGCTGTTCA

ACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACT

GCCTGGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTC

AACGGCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGC

CCAGTACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGA

CCTTCGGCGCCGGCGCCGCCCTGCAGATCCCCTTCGCCATGCAGATG

GCCTACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAG

AACCAGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATC

CAGGACAGCCTGAGCAGCACCGCCAGCGCCCTGGGCAAGCTGCAGGA

CGTGGTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGC

TGAGCAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGA

GCAGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATC

ACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGAT

CAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGA

TGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGC

AAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGT

GGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCAC

CACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGG

AGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGG

AACTTCTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGC

GGCAACTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGAC

CCCCTGCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTA

CTTCAAGAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCG

GCATCAACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGA

ACGAGGTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAG

CTGGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTG

GGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTG

TGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGC

GGCAGCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAA

GGGCGTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCT

GTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTAGCGCCGT

GAAGAGGACCATCAAGGGCACCCACCACTGGCTGCTGCTGACCATCCT

GACCAGCCTGCTGGTGCTGGTGCAGAGCACCCAGTGGAGCCTGTTCTT

CTTCCTGTACGAGAACGCCTTCCTGCCCTTCGCCATGGGCATCATCGC

CATGAGCGCCTTCGCCATGATGTTCGTGAAGCACAAGCACGCCTTCCT

GTGCCTGTTCCTGCTGCCCAGCCTGGCCACCGTGGCCTACTTCAACAT

GGTGTACATGCCCGCCAGCTGGGTGATGAGGATCATGACCTGGCTGGA

CATGGTGGACACCAGCCTGAGCGGCTTCAAGCTGAAGGACTGCGTGAT

GTACGCCAGCGCCGTGGTGCTGCTGATCCTGATGACCGCCAGGACCG

TGTACGACGACGGCGCCAGGAGGGTGTGGACCCTGATGAACGTGCTG

ACCCTGGTGTACAAGGTGTACTACGGCAACGCCCTGGACCAGGCCATC

AGCATGTGGGCCCTGATCATCAGCGTGACCAGCAACTACAGCGGCGTG

GTGACCACCGTGATGTTCCTGGCCAGGGGCATCGTGTTCATGTGCGTG

GAGTACTGCCCCATCTTCTTCATCACCGGCAACACCCTGCAGTGCATCA

TGCTGGTGTACTGCTTCCTGGGCTACTTCTGCACCTGCTACTTCGGCCT

GTTCTGCCTGCTGAACAGGTACTTCAGGCTGACCCTGGGCGTGTACGA

CTACCTGGTGAGCACCCAGGAGTTCAGGTACATGAACAGCCAGGGCCT

GCTGCCCCCCAAGAACAGCATCGACGCCTTCAAGCTGAACATCAAGCT

GCTGGGCGTGGGCGGCAAGCCCTGCATCAAGGTGGCCACCGTGCAGG

GAAGCGGAGAGGGCAGGGGAAGTCTTCTAACATGCGGGGACGTGGAG

GAAAATCCCGGCCCCAGCAAGATGAGCGACGTGAAGTGCACCAGCGT

GGTGCTGCTGAGCGTGCTGCAGCAGCTGAGGGTGGAGAGCAGCAGCA

AGCTGTGGGCCCAGTGCGTGCAGCTGCACAACGACATCCTGCTGGCC

AAGGACACCACCGAGGCCTTCGAGAAGATGGTGAGCCTGCTGAGCGT

GCTGCTGAGCATGCAGGGCGCCGTGGACATCAACAAGCTGTGCGAGG

AGATGCTGGACAACAGGGCCACCCTGCAGGGAAGCGGACAGTGTACT

AATTATGCTCTCTTGAAATTGGCTGGAGATGTTGAGAGCAACCCAGGTC

CCGCCATCGCCAGCGAGTTCAGCAGCCTGCCCAGCTACGCCGCCTTC

GCCACCGCCCAGGAGGCCTACGAGCAGGCCGTGGCCAACGGCGACA

GCGAGGTGGTGCTGAAGAAGCTGAAGAAGAGCCTGAACGTGGCCAAG

AGCGAGTTCGACAGGGACGCCGCCATGCAGAGGAAGCTGGAGAAGAT

GGCCGACCAGGCCATGACCCAGATGTACAAGCAGGCCAGGAGCGAGG

ACAAGAGGGCCAAGGTGACCAGCGCCATGCAGACCATGCTGTTCACCA

TGCTGAGGAAGCTGGACAACGACGCCCTGAACAACATCATCAACAACG

CCAGGGACGGCTGCGTGCCCCTGAACATCATCCCCCTGACCACCGCC

GCCAAGCTGATGGTGGTGATCCCCGACTACAACACCTACAAGAACACC

TGCGACGGCACCACCTTCACCTACGCCAGCGCCCTGTGGGAGATCCA

GCAGGTGGTGGACGCCGACAGCAAGATCGTGCAGCTGAGCGAGATCA

GCATGGACAACAGCCCCAACCTGGCCTGGCCCCTGATCGTGACCGCC

CTGAGGGCCAACAGCGCCGTGAAGCTGCAGTGATGACTCGAGCTGGT

ACTGCATGCACGCAATGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCC

GAGTCTCCCCCGACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGC

CCCACTCACCACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCA

ATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCA

GTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCC

CAGGGTTGGTCAATTTCGTGCCAGCCACACCCTGGAGCTAGCAAAAAA

AA

13
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA
298

Ad5-Pan-CoV
TGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGAC

HexaPro-
CGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT

NSP6-NSP7-
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA

NSP8
CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTA

CGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATG

CCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT

ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAAT

GGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCC

ATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTC

CAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGC

GTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTC

AGATCGAGAATAAACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGA

ACCCGCCACCATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAG

CCAGTGCGTGAACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACA

CCAACAGCTTCACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGA

GCAGCGTGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCA

ACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCA

AGAGGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCG

CCAGCACCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCA

CCCTGGACAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCA

ACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCC

TGGGCGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGT

TCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCC

AGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACC

TGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAG

CAAGCACACCCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAG

CGCCCTGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAG

GTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCG

ACAGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGC

TACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACC

ATCACCGACGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAA

GTGCACCCTGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAG

CAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACAT

CACCAACCTGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGC

CAGCGTGTACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCG

ACTACAGCGTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCT

ACGGCGTGAGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGT

ACGCCGACAGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCC

CCCGGCCAGACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGA

CGACTTCACCGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAG

CAAGGTGGGCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAG

CAACCTGAAGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGC

CGGCAGCACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCC

CCTGCAGAGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGC

CCTACAGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCC

ACCGTGTGCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGC

GTGAACTTCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAG

AGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCC

GACACCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGA

CATCACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCA

CCAACACCAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCA

CCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGG

AGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTG

CCTGATCGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCC

CATCGGCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCC

CCGGCAGCGCCAGCAGCGTGGCCAGCCAGAGCATCATCGCCTACACC

ATGAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCAT

CGCCATCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCC

CGTGAGCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGG

CGACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTG

CACCCAGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACA

AGAACACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCC

CCCCCATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCG

ACCCCAGCAAGCCCAGCAAGAGGAGCCCCATCGAGGACCTGCTGTTCA

ACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACT

GCCTGGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTC

AACGGCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGC

CCAGTACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGA

CCTTCGGCGCCGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATG

GCCTACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAG

AACCAGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATC

CAGGACAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGA

CGTGGTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGC

TGAGCAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGA

GCAGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATC

ACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGAT

CAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGA

TGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGC

AAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGT

GGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCAC

CACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGG

AGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGG

AACTTCTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGC

GGCAACTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGAC

CCCCTGCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTA

CTTCAAGAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCG

GCATCAACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGA

ACGAGGTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAG

CTGGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTG

GGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTG

TGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGC

GGCAGCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAA

GGGCGTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCT

GTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTAGCGCCGT

GAAGAGGACCATCAAGGGCACCCACCACTGGCTGCTGCTGACCATCCT

GACCAGCCTGCTGGTGCTGGTGCAGAGCACCCAGTGGAGCCTGTTCTT

CTTCCTGTACGAGAACGCCTTCCTGCCCTTCGCCATGGGCATCATCGC

CATGAGCGCCTTCGCCATGATGTTCGTGAAGCACAAGCACGCCTTCCT

GTGCCTGTTCCTGCTGCCCAGCCTGGCCACCGTGGCCTACTTCAACAT

GGTGTACATGCCCGCCAGCTGGGTGATGAGGATCATGACCTGGCTGGA

CATGGTGGACACCAGCCTGAGCGGCTTCAAGCTGAAGGACTGCGTGAT

GTACGCCAGCGCCGTGGTGCTGCTGATCCTGATGACCGCCAGGACCG

TGTACGACGACGGCGCCAGGAGGGTGTGGACCCTGATGAACGTGCTG

ACCCTGGTGTACAAGGTGTACTACGGCAACGCCCTGGACCAGGCCATC

AGCATGTGGGCCCTGATCATCAGCGTGACCAGCAACTACAGCGGCGTG

GTGACCACCGTGATGTTCCTGGCCAGGGGCATCGTGTTCATGTGCGTG

GAGTACTGCCCCATCTTCTTCATCACCGGCAACACCCTGCAGTGCATCA

TGCTGGTGTACTGCTTCCTGGGCTACTTCTGCACCTGCTACTTCGGCCT

GTTCTGCCTGCTGAACAGGTACTTCAGGCTGACCCTGGGCGTGTACGA

CTACCTGGTGAGCACCCAGGAGTTCAGGTACATGAACAGCCAGGGCCT

GCTGCCCCCCAAGAACAGCATCGACGCCTTCAAGCTGAACATCAAGCT

GCTGGGCGTGGGCGGCAAGCCCTGCATCAAGGTGGCCACCGTGCAGG

GAAGCGGAGAGGGCAGGGGAAGTCTTCTAACATGCGGGGACGTGGAG

GAAAATCCCGGCCCCAGCAAGATGAGCGACGTGAAGTGCACCAGCGT

GGTGCTGCTGAGCGTGCTGCAGCAGCTGAGGGTGGAGAGCAGCAGCA

AGCTGTGGGCCCAGTGCGTGCAGCTGCACAACGACATCCTGCTGGCC

AAGGACACCACCGAGGCCTTCGAGAAGATGGTGAGCCTGCTGAGCGT

GCTGCTGAGCATGCAGGGCGCCGTGGACATCAACAAGCTGTGCGAGG

AGATGCTGGACAACAGGGCCACCCTGCAGGGAAGCGGACAGTGTACT

AATTATGCTCTCTTGAAATTGGCTGGAGATGTTGAGAGCAACCCAGGTC

CCGCCATCGCCAGCGAGTTCAGCAGCCTGCCCAGCTACGCCGCCTTC

GCCACCGCCCAGGAGGCCTACGAGCAGGCCGTGGCCAACGGCGACA

GCGAGGTGGTGCTGAAGAAGCTGAAGAAGAGCCTGAACGTGGCCAAG

AGCGAGTTCGACAGGGACGCCGCCATGCAGAGGAAGCTGGAGAAGAT

GGCCGACCAGGCCATGACCCAGATGTACAAGCAGGCCAGGAGCGAGG

ACAAGAGGGCCAAGGTGACCAGCGCCATGCAGACCATGCTGTTCACCA

TGCTGAGGAAGCTGGACAACGACGCCCTGAACAACATCATCAACAACG

CCAGGGACGGCTGCGTGCCCCTGAACATCATCCCCCTGACCACCGCC

GCCAAGCTGATGGTGGTGATCCCCGACTACAACACCTACAAGAACACC

TGCGACGGCACCACCTTCACCTACGCCAGCGCCCTGTGGGAGATCCA

GCAGGTGGTGGACGCCGACAGCAAGATCGTGCAGCTGAGCGAGATCA

GCATGGACAACAGCCCCAACCTGGCCTGGCCCCTGATCGTGACCGCC

CTGAGGGCCAACAGCGCCGTGAAGCTGCAGTGATGACTCGAGCTGGT

ACTGCATGCACGCAATGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCC

GAGTCTCCCCCGACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGC

CCCACTCACCACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCA

ATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCA

GTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCC

CAGGGTTGGTCAATTTCGTGCCAGCCACACCCTGGAGCTAGCAAAAAA

AA

14
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA
299

Ad5-Pan-CoV
TGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGAC

Spike-NSP9-
CGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT

NSP10-
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA

NSP13
CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTA

CGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATG

CCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT

ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAAT

GGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCC

ATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTC

CAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGC

GTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTC

AGATCGAGAATAAACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGA

ACCCGCCACCATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAG

CCAGTGCGTGAACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACA

CCAACAGCTTCACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGA

GCAGCGTGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCA

ACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCA

AGAGGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCG

CCAGCACCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCA

CCCTGGACAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCA

ACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCC

TGGGCGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGT

TCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCC

AGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACC

TGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAG

CAAGCACACCCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAG

CGCCCTGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAG

GTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCG

ACAGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGC

TACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACC

ATCACCGACGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAA

GTGCACCCTGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAG

CAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACAT

CACCAACCTGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGC

CAGCGTGTACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCG

ACTACAGCGTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCT

ACGGCGTGAGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGT

ACGCCGACAGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCC

CCCGGCCAGACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGA

CGACTTCACCGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAG

CAAGGTGGGCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAG

CAACCTGAAGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGC

CGGCAGCACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCC

CCTGCAGAGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGC

CCTACAGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCC

ACCGTGTGCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGC

GTGAACTTCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAG

AGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCC

GACACCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGA

CATCACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCA

CCAACACCAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCA

CCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGG

AGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTG

CCTGATCGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCC

CATCGGCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCC

CCAGGAGGGCCAGGAGCGTGGCCAGCCAGAGCATCATCGCCTACACC

ATGAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCAT

CGCCATCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCC

CGTGAGCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGG

CGACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTG

CACCCAGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACA

AGAACACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCC

CCCCCATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCG

ACCCCAGCAAGCCCAGCAAGAGGAGCTTCATCGAGGACCTGCTGTTCA

ACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACT

GCCTGGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTC

AACGGCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGC

CCAGTACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGA

CCTTCGGCGCCGGCGCCGCCCTGCAGATCCCCTTCGCCATGCAGATG

GCCTACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAG

AACCAGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATC

CAGGACAGCCTGAGCAGCACCGCCAGCGCCCTGGGCAAGCTGCAGGA

CGTGGTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGC

TGAGCAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGA

GCAGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATC

ACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGAT

CAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGA

TGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGC

AAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGT

GGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCAC

CACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGG

AGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGG

AACTTCTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGC

GGCAACTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGAC

CCCCTGCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTA

CTTCAAGAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCG

GCATCAACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGA

ACGAGGTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAG

CTGGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTG

GGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTG

TGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGC

GGCAGCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAA

GGGCGTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCT

GTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTAACAACGA

GCTGAGCCCCGTGGCCCTGAGGCAGATGAGCTGCGCCGCCGGCACCA

CCCAGACCGCCTGCACCGACGACAACGCCCTGGCCTACTACAACACCA

CCAAGGGCGGCAGGTTCGTGCTGGCCCTGCTGAGCGACCTGCAGGAC

CTGAAGTGGGCCAGGTTCCCCAAGAGCGACGGCACCGGCACCATCTA

CACCGAGCTGGAGCCCCCCTGCAGGTTCGTGACCGACACCCCCAAGG

GCCCCAAGGTGAAGTACCTGTACTTCATCAAGGGCCTGAACAACCTGA

ACAGGGGCATGGTGCTGGGCAGCCTGGCCGCCACCGTGAGGCTGCAG

GGAAGCGGAGAGGGCAGGGGAAGTCTTCTAACATGCGGGGACGTGGA

GGAAAATCCCGGCCCCGCCGGCAACGCCACCGAGGTGCCCGCCAACA

GCACCGTGCTGAGCTTCTGCGCCTTCGCCGTGGACGCCGCCAAGGCC

TACAAGGACTACCTGGCCAGCGGCGGCCAGCCCATCACCAACTGCGT

GAAGATGCTGTGCACCCACACCGGCACCGGCCAGGCCATCACCGTGA

CCCCCGAGGCCAACATGGACCAGGAGAGCTTCGGCGGCGCCAGCTGC

TGCCTGTACTGCAGGTGCCACATCGACCACCCCAACCCCAAGGGCTTC

TGCGACCTGAAGGGCAAGTACGTGCAGATCCCCACCACCTGCGCCAAC

GACCCCGTGGGCTTCACCCTGAAGAACACCGTGTGCACCGTGTGCGG

CATGTGGAAGGGCTACGGCTGCAGCTGCGACCAGCTGAGGGAGCCCA

TGCTGCAGGGAAGCGGACAGTGTACTAATTATGCTCTCTTGAAATTGGC

TGGAGATGTTGAGAGCAACCCAGGTCCCGCCGTGGGCGCCTGCGTGC

TGTGCAACAGCCAGACCAGCCTGAGGTGCGGCGCCTGCATCAGGAGG

CCCTTCCTGTGCTGCAAGTGCTGCTACGACCACGTGATCAGCACCAGC

CACAAGCTGGTGCTGAGCGTGAACCCCTACGTGTGCAACGCCCCCGG

CTGCGACGTGACCGACGTGACCCAGCTGTACCTGGGCGGCATGAGCT

ACTACTGCAAGAGCCACAAGCCCCCCATCAGCTTCCCCCTGTGCGCCA

ACGGCCAGGTGTTCGGCCTGTACAAGAACACCTGCGTGGGCAGCGAC

AACGTGACCGACTTCAACGCCATCGCCACCTGCGACTGGACCAACGCC

GGCGACTACATCCTGGCCAACACCTGCACCGAGAGGCTGAAGCTGTTC

GCCGCCGAGACCCTGAAGGCCACCGAGGAGACCTTCAAGCTGAGCTA

CGGCATCGCCACCGTGAGGGAGGTGCTGAGCGACAGGGAGCTGCACC

TGAGCTGGGAGGTGGGCAAGCCCAGGCCCCCCCTGAACAGGAACTAC

GTGTTCACCGGCTACAGGGTGACCAAGAACAGCAAGGTGCAGATCGGC

GAGTACACCTTCGAGAAGGGCGACTACGGCGACGCCGTGGTGTACAG

GGGCACCACCACCTACAAGCTGAACGTGGGCGACTACTTCGTGCTGAC

CAGCCACACCGTGATGCCCCTGAGCGCCCCCACCCTGGTGCCCCAGG

AGCACTACGTGAGGATCACCGGCCTGTACCCCACCCTGAACATCAGCG

ACGAGTTCAGCAGCAACGTGGCCAACTACCAGAAGGTGGGCATGCAGA

AGTACAGCACCCTGCAGGGCCCCCCCGGCACCGGCAAGAGCCACTTC

GCCATCGGCCTGGCCCTGTACTACCCCAGCGCCAGGATCGTGTACACC

GCCTGCAGCCACGCCGCCGTGGACGCCCTGTGCGAGAAGGCCCTGAA

GTACCTGCCCATCGACAAGTGCAGCAGGATCATCCCCGCCAGGGCCA

GGGTGGAGTGCTTCGACAAGTTCAAGGTGAACAGCACCCTGGAGCAGT

ACGTGTTCTGCACCGTGAACGCCCTGCCCGAGACCACCGCCGACATCG

TGGTGTTCGACGAGATCAGCATGGCCACCAACTACGACCTGAGCGTGG

TGAACGCCAGGCTGAGGGCCAAGCACTACGTGTACATCGGCGACCCC

GCCCAGCTGCCCGCCCCCAGGACCCTGCTGACCAAGGGCACCCTGGA

GCCCGAGTACTTCAACAGCGTGTGCAGGCTGATGAAGACCATCGGCCC

CGACATGTTCCTGGGCACCTGCAGGAGGTGCCCCGCCGAGATCGTGG

ACACCGTGAGCGCCCTGGTGTACGACAACAAGCTGAAGGCCCACAAG

GACAAGAGCGCCCAGTGCTTCAAGATGTTCTACAAGGGCGTGATCACC

CACGACGTGAGCAGCGCCATCAACAGGCCCCAGATCGGCGTGGTGAG

GGAGTTCCTGACCAGGAACCCCGCCTGGAGGAAGGCCGTGTTCATCA

GCCCCTACAACAGCCAGAACGCCGTGGCCAGCAAGATCCTGGGCCTG

CCCACCCAGACCGTGGACAGCAGCCAGGGCAGCGAGTACGACTACGT

GATCTTCACCCAGACCACCGAGACCGCCCACAGCTGCAACGTGAACAG

GTTCAACGTGGCCATCACCAGGGCCAAGGTGGGCATCCTGTGCATCAT

GAGCGACAGGGACCTGTACGACAAGCTGCAGTTCACCAGCCTGGAGAT

CCCCAGGAGGAACGTGGCCACCCTGCAGTGATGACTCGAGCTGGTACT

GCATGCACGCAATGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCCGAG

TCTCCCCCGACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGCCCC

ACTCACCACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCAATG

CAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTG

ATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAG

GGTTGGTCAATTTCGTGCCAGCCACACCCTGGAGCTAGCAAAAAAAA

15
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA
300

Ad5-Pan-CoV
TGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGAC

HexaPro-
CGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT

NSP9-NSP10-
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA

NSP13
CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTA

CGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATG

CCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT

ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAAT

GGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCC

ATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTC

CAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGC

GTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTC

AGATCGAGAATAAACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGA

ACCCGCCACCATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAG

CCAGTGCGTGAACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACA

CCAACAGCTTCACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGA

GCAGCGTGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCA

ACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCA

AGAGGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCG

CCAGCACCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCA

CCCTGGACAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCA

ACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCC

TGGGCGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGT

TCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCC

AGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACC

TGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAG

CAAGCACACCCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAG

CGCCCTGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAG

GTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCG

ACAGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGC

TACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACC

ATCACCGACGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAA

GTGCACCCTGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAG

CAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACAT

CACCAACCTGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGC

CAGCGTGTACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCG

ACTACAGCGTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCT

ACGGCGTGAGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGT

ACGCCGACAGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCC

CCCGGCCAGACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGA

CGACTTCACCGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAG

CAAGGTGGGCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAG

CAACCTGAAGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGC

CGGCAGCACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCC

CCTGCAGAGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGC

CCTACAGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCC

ACCGTGTGCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGC

GTGAACTTCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAG

AGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCC

GACACCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGA

CATCACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCA

CCAACACCAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCA

CCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGG

AGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTG

CCTGATCGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCC

CATCGGCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCC

CCGGCAGCGCCAGCAGCGTGGCCAGCCAGAGCATCATCGCCTACACC

ATGAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCAT

CGCCATCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCC

CGTGAGCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGG

CGACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTG

CACCCAGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACA

AGAACACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCC

CCCCCATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCG

ACCCCAGCAAGCCCAGCAAGAGGAGCCCCATCGAGGACCTGCTGTTCA

ACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACT

GCCTGGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTC

AACGGCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGC

CCAGTACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGA

CCTTCGGCGCCGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATG

GCCTACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAG

AACCAGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATC

CAGGACAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGA

CGTGGTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGC

TGAGCAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGA

GCAGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATC

ACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGAT

CAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGA

TGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGC

AAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGT

GGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCAC

CACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGG

AGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGG

AACTTCTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGC

GGCAACTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGAC

CCCCTGCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTA

CTTCAAGAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCG

GCATCAACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGA

ACGAGGTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAG

CTGGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTG

GGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTG

TGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGC

GGCAGCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAA

GGGCGTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCT

GTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTAACAACGA

GCTGAGCCCCGTGGCCCTGAGGCAGATGAGCTGCGCCGCCGGCACCA

CCCAGACCGCCTGCACCGACGACAACGCCCTGGCCTACTACAACACCA

CCAAGGGCGGCAGGTTCGTGCTGGCCCTGCTGAGCGACCTGCAGGAC

CTGAAGTGGGCCAGGTTCCCCAAGAGCGACGGCACCGGCACCATCTA

CACCGAGCTGGAGCCCCCCTGCAGGTTCGTGACCGACACCCCCAAGG

GCCCCAAGGTGAAGTACCTGTACTTCATCAAGGGCCTGAACAACCTGA

ACAGGGGCATGGTGCTGGGCAGCCTGGCCGCCACCGTGAGGCTGCAG

GGAAGCGGAGAGGGCAGGGGAAGTCTTCTAACATGCGGGGACGTGGA

GGAAAATCCCGGCCCCGCCGGCAACGCCACCGAGGTGCCCGCCAACA

GCACCGTGCTGAGCTTCTGCGCCTTCGCCGTGGACGCCGCCAAGGCC

TACAAGGACTACCTGGCCAGCGGCGGCCAGCCCATCACCAACTGCGT

GAAGATGCTGTGCACCCACACCGGCACCGGCCAGGCCATCACCGTGA

CCCCCGAGGCCAACATGGACCAGGAGAGCTTCGGCGGCGCCAGCTGC

TGCCTGTACTGCAGGTGCCACATCGACCACCCCAACCCCAAGGGCTTC

TGCGACCTGAAGGGCAAGTACGTGCAGATCCCCACCACCTGCGCCAAC

GACCCCGTGGGCTTCACCCTGAAGAACACCGTGTGCACCGTGTGCGG

CATGTGGAAGGGCTACGGCTGCAGCTGCGACCAGCTGAGGGAGCCCA

TGCTGCAGGGAAGCGGACAGTGTACTAATTATGCTCTCTTGAAATTGGC

TGGAGATGTTGAGAGCAACCCAGGTCCCGCCGTGGGCGCCTGCGTGC

TGTGCAACAGCCAGACCAGCCTGAGGTGCGGCGCCTGCATCAGGAGG

CCCTTCCTGTGCTGCAAGTGCTGCTACGACCACGTGATCAGCACCAGC

CACAAGCTGGTGCTGAGCGTGAACCCCTACGTGTGCAACGCCCCCGG

CTGCGACGTGACCGACGTGACCCAGCTGTACCTGGGCGGCATGAGCT

ACTACTGCAAGAGCCACAAGCCCCCCATCAGCTTCCCCCTGTGCGCCA

ACGGCCAGGTGTTCGGCCTGTACAAGAACACCTGCGTGGGCAGCGAC

AACGTGACCGACTTCAACGCCATCGCCACCTGCGACTGGACCAACGCC

GGCGACTACATCCTGGCCAACACCTGCACCGAGAGGCTGAAGCTGTTC

GCCGCCGAGACCCTGAAGGCCACCGAGGAGACCTTCAAGCTGAGCTA

CGGCATCGCCACCGTGAGGGAGGTGCTGAGCGACAGGGAGCTGCACC

TGAGCTGGGAGGTGGGCAAGCCCAGGCCCCCCCTGAACAGGAACTAC

GTGTTCACCGGCTACAGGGTGACCAAGAACAGCAAGGTGCAGATCGGC

GAGTACACCTTCGAGAAGGGCGACTACGGCGACGCCGTGGTGTACAG

GGGCACCACCACCTACAAGCTGAACGTGGGCGACTACTTCGTGCTGAC

CAGCCACACCGTGATGCCCCTGAGCGCCCCCACCCTGGTGCCCCAGG

AGCACTACGTGAGGATCACCGGCCTGTACCCCACCCTGAACATCAGCG

ACGAGTTCAGCAGCAACGTGGCCAACTACCAGAAGGTGGGCATGCAGA

AGTACAGCACCCTGCAGGGCCCCCCCGGCACCGGCAAGAGCCACTTC

GCCATCGGCCTGGCCCTGTACTACCCCAGCGCCAGGATCGTGTACACC

GCCTGCAGCCACGCCGCCGTGGACGCCCTGTGCGAGAAGGCCCTGAA

GTACCTGCCCATCGACAAGTGCAGCAGGATCATCCCCGCCAGGGCCA

GGGTGGAGTGCTTCGACAAGTTCAAGGTGAACAGCACCCTGGAGCAGT

ACGTGTTCTGCACCGTGAACGCCCTGCCCGAGACCACCGCCGACATCG

TGGTGTTCGACGAGATCAGCATGGCCACCAACTACGACCTGAGCGTGG

TGAACGCCAGGCTGAGGGCCAAGCACTACGTGTACATCGGCGACCCC

GCCCAGCTGCCCGCCCCCAGGACCCTGCTGACCAAGGGCACCCTGGA

GCCCGAGTACTTCAACAGCGTGTGCAGGCTGATGAAGACCATCGGCCC

CGACATGTTCCTGGGCACCTGCAGGAGGTGCCCCGCCGAGATCGTGG

ACACCGTGAGCGCCCTGGTGTACGACAACAAGCTGAAGGCCCACAAG

GACAAGAGCGCCCAGTGCTTCAAGATGTTCTACAAGGGCGTGATCACC

CACGACGTGAGCAGCGCCATCAACAGGCCCCAGATCGGCGTGGTGAG

GGAGTTCCTGACCAGGAACCCCGCCTGGAGGAAGGCCGTGTTCATCA

GCCCCTACAACAGCCAGAACGCCGTGGCCAGCAAGATCCTGGGCCTG

CCCACCCAGACCGTGGACAGCAGCCAGGGCAGCGAGTACGACTACGT

GATCTTCACCCAGACCACCGAGACCGCCCACAGCTGCAACGTGAACAG

GTTCAACGTGGCCATCACCAGGGCCAAGGTGGGCATCCTGTGCATCAT

GAGCGACAGGGACCTGTACGACAAGCTGCAGTTCACCAGCCTGGAGAT

CCCCAGGAGGAACGTGGCCACCCTGCAGTGATGACTCGAGCTGGTACT

GCATGCACGCAATGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCCGAG

TCTCCCCCGACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGCCCC

ACTCACCACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCAATG

CAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTG

ATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAG

GGTTGGTCAATTTCGTGCCAGCCACACCCTGGAGCTAGCAAAAAAAA

16
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA
301

Ad5-Pan-CoV
TGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGAC

Spike-NSP14-
CGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT

NSP15
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA

CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTA

CGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATG

CCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT

ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAAT

GGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCC

ATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTC

CAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGC

GTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTC

AGATCGAGAATAAACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGA

ACCCGCCACCATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAG

CCAGTGCGTGAACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACA

CCAACAGCTTCACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGA

GCAGCGTGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCA

ACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCA

AGAGGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCG

CCAGCACCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCA

CCCTGGACAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCA

ACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCC

TGGGCGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGT

TCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCC

AGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACC

TGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAG

CAAGCACACCCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAG

CGCCCTGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAG

GTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCG

ACAGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGC

TACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACC

ATCACCGACGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAA

GTGCACCCTGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAG

CAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACAT

CACCAACCTGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGC

CAGCGTGTACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCG

ACTACAGCGTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCT

ACGGCGTGAGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGT

ACGCCGACAGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCC

CCCGGCCAGACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGA

CGACTTCACCGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAG

CAAGGTGGGCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAG

CAACCTGAAGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGC

CGGCAGCACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCC

CCTGCAGAGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGC

CCTACAGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCC

ACCGTGTGCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGC

GTGAACTTCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAG

AGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCC

GACACCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGA

CATCACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCA

CCAACACCAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCA

CCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGG

AGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTG

CCTGATCGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCC

CATCGGCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCC

CCAGGAGGGCCAGGAGCGTGGCCAGCCAGAGCATCATCGCCTACACC

ATGAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCAT

CGCCATCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCC

CGTGAGCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGG

CGACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTG

CACCCAGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACA

AGAACACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCC

CCCCCATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCG

ACCCCAGCAAGCCCAGCAAGAGGAGCTTCATCGAGGACCTGCTGTTCA

ACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACT

GCCTGGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTC

AACGGCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGC

CCAGTACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGA

CCTTCGGCGCCGGCGCCGCCCTGCAGATCCCCTTCGCCATGCAGATG

GCCTACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAG

AACCAGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATC

CAGGACAGCCTGAGCAGCACCGCCAGCGCCCTGGGCAAGCTGCAGGA

CGTGGTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGC

TGAGCAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGA

GCAGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATC

ACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGAT

CAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGA

TGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGC

AAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGT

GGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCAC

CACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGG

AGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGG

AACTTCTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGC

GGCAACTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGAC

CCCCTGCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTA

CTTCAAGAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCG

GCATCAACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGA

ACGAGGTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAG

CTGGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTG

GGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTG

TGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGC

GGCAGCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAA

GGGCGTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCT

GTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTGCCGAGAA

CGTGACCGGCCTGTTCAAGGACTGCAGCAAGGTGATCACCGGCCTGCA

CCCCACCCAGGCCCCCACCCACCTGAGCGTGGACACCAAGTTCAAGAC

CGAGGGCCTGTGCGTGGACATCCCCGGCATCCCCAAGGACATGACCT

ACAGGAGGCTGATCAGCATGATGGGCTTCAAGATGAACTACCAGGTGA

ACGGCTACCCCAACATGTTCATCACCAGGGAGGAGGCCATCAGGCACG

TGAGGGCCTGGATCGGCTTCGACGTGGAGGGCTGCCACGCCACCAGG

GAGGCCGTGGGCACCAACCTGCCCCTGCAGCTGGGCTTCAGCACCGG

CGTGAACCTGGTGGCCGTGCCCACCGGCTACGTGGACACCCCCAACA

ACACCGACTTCAGCAGGGTGAGCGCCAAGCCCCCCCCCGGCGACCAG

TTCAAGCACCTGATCCCCCTGATGTACAAGGGCCTGCCCTGGAACGTG

GTGAGGATCAAGATCGTGCAGATGCTGAGCGACACCCTGAAGAACCTG

AGCGACAGGGTGGTGTTCGTGCTGTGGGCCCACGGCTTCGAGCTGAC

CAGCATGAAGTACTTCGTGAAGATCGGCCCCGAGAGGACCTGCTGCCT

GTGCGACAGGAGGGCCACCTGCTTCAGCACCGCCAGCGACACCTACG

CCTGCTGGCACCACAGCATCGGCTTCGACTACGTGTACAACCCCTTCA

TGATCGACGTGCAGCAGTGGGGCTTCACCGGCAACCTGCAGAGCAAC

CACGACCTGTACTGCCAGGTGCACGGCAACGCCCACGTGGCCAGCTG

CGACGCCATCATGACCAGGTGCCTGGCCGTGCACGAGTGCTTCGTGAA

GAGGGTGGACTGGACCATCGAGTACCCCATCATCGGCGACGAGCTGA

AGATCAACGCCGCCTGCAGGAAGGTGCAGCACATGGTGGTGAAGGCC

GCCCTGCTGGCCGACAAGTTCCCCGTGCTGCACGACATCGGCAACCC

CAAGGCCATCAAGTGCGTGCCCCAGGCCGACGTGGAGTGGAAGTTCTA

CGACGCCCAGCCCTGCAGCGACAAGGCCTACAAGATCGAGGAGCTGT

TCTACAGCTACGCCACCCACAGCGACAAGTTCACCGACGGCGTGTGCC

TGTTCTGGAACTGCAACGTGGACAGGTACCCCGCCAACAGCATCGTGT

GCAGGTTCGACACCAGGGTGCTGAGCAACCTGAACCTGCCCGGCTGC

GACGGCGGCAGCCTGTACGTGAACAAGCACGCCTTCCACACCCCCGC

CTTCGACAAGAGCGCCTTCGTGAACCTGAAGCAGCTGCCCTTCTTCTAC

TACAGCGACAGCCCCTGCGAGAGCCACGGCAAGCAGGTGGTGAGCGA

CATCGACTACGTGCCCCTGAAGAGCGCCACCTGCATCACCAGGTGCAA

CCTGGGCGGCGCCGTGTGCAGGCACCACGCCAACGAGTACAGGCTGT

ACCTGGACGCCTACAACATGATGATCAGCGCCGGCTTCAGCCTGTGGG

TGTACAAGCAGTTCGACACCTACAACCTGTGGAACACCTTCACCAGGCT

GCAGGGAAGCGGAGAGGGCAGGGGAAGTCTTCTAACATGCGGGGACG

TGGAGGAAAATCCCGGCCCCAGCCTGGAGAACGTGGCCTTCAACGTG

GTGAACAAGGGCCACTTCGACGGCCAGCAGGGCGAGGTGCCCGTGAG

CATCATCAACAACACCGTGTACACCAAGGTGGACGGCGTGGACGTGGA

GCTGTTCGAGAACAAGACCACCCTGCCCGTGAACGTGGCCTTCGAGCT

GTGGGCCAAGAGGAACATCAAGCCCGTGCCCGAGGTGAAGATCCTGA

ACAACCTGGGCGTGGACATCGCCGCCAACACCGTGATCTGGGACTACA

AGAGGGACGCCCCCGCCCACATCAGCACCATCGGCGTGTGCAGCATG

ACCGACATCGCCAAGAAGCCCACCGAGACCATCTGCGCCCCCCTGACC

GTGTTCTTCGACGGCAGGGTGGACGGCCAGGTGGACCTGTTCAGGAA

CGCCAGGAACGGCGTGCTGATCACCGAGGGCAGCGTGAAGGGCCTGC

AGCCCAGCGTGGGCCCCAAGCAGGCCAGCCTGAACGGCGTGACCCTG

ATCGGCGAGGCCGTGAAGACCCAGTTCAACTACTACAAGAAGGTGGAC

GGCGTGGTGCAGCAGCTGCCCGAGACCTACTTCACCCAGAGCAGGAA

CCTGCAGGAGTTCAAGCCCAGGAGCCAGATGGAGATCGACTTCCTGGA

GCTGGCCATGGACGAGTTCATCGAGAGGTACAAGCTGGAGGGCTACG

CCTTCGAGCACATCGTGTACGGCGACTTCAGCCACAGCCAGCTGGGCG

GCCTGCACCTGCTGATCGGCCTGGCCAAGAGGTTCAAGGAGAGCCCC

TTCGAGCTGGAGGACTTCATCCCCATGGACAGCACCGTGAAGAACTAC

TTCATCACCGACGCCCAGACCGGCAGCAGCAAGTGCGTGTGCAGCGT

GATCGACCTGCTGCTGGACGACTTCGTGGAGATCATCAAGAGCCAGGA

CCTGAGCGTGGTGAGCAAGGTGGTGAAGGTGACCATCGACTACACCGA

GATCAGCTTCATGCTGTGGTGCAAGGACGGCCACGTGGAGACCTTCTA

CCCCAAGCTGCAGTGATGACTCGAGCTGGTACTGCATGCACGCAATGC

TAGCTGCCCCTTTCCCGTCCTGGGTACCCCGAGTCTCCCCCGACCTCG

GGTCCCAGGTATGCTCCCACCTCCACCTGCCCCACTCACCACCTCTGC

TAGTTCCAGACACCTCCCAAGCACGCAGCAATGCAGCTCAAAACGCTTA

GCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAAT

AAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCG

TGCCAGCCACACCCTGGAGCTAGCAAAAAAAA

17
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA
302

Ad5-Pan-CoV
TGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGAC

HexaPro-
CGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT

NSP14-
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA

NSP15
CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTA

CGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATG

CCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT

ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAAT

GGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCC

ATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTC

CAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGC

GTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTC

AGATCGAGAATAAACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGA

ACCCGCCACCATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAG

CCAGTGCGTGAACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACA

CCAACAGCTTCACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGA

GCAGCGTGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCA

ACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCA

AGAGGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCG

CCAGCACCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCA

CCCTGGACAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCA

ACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCC

TGGGCGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGT

TCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCC

AGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACC

TGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAG

CAAGCACACCCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAG

CGCCCTGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAG

GTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCG

ACAGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGC

TACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACC

ATCACCGACGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAA

GTGCACCCTGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAG

CAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACAT

CACCAACCTGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGC

CAGCGTGTACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCG

ACTACAGCGTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCT

ACGGCGTGAGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGT

ACGCCGACAGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCC

CCCGGCCAGACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGA

CGACTTCACCGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAG

CAAGGTGGGCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAG

CAACCTGAAGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGC

CGGCAGCACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCC

CCTGCAGAGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGC

CCTACAGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCC

ACCGTGTGCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGC

GTGAACTTCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAG

AGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCC

GACACCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGA

CATCACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCA

CCAACACCAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCA

CCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGG

AGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTG

CCTGATCGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCC

CATCGGCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCC

CCGGCAGCGCCAGCAGCGTGGCCAGCCAGAGCATCATCGCCTACACC

ATGAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCAT

CGCCATCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCC

CGTGAGCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGG

CGACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTG

CACCCAGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACA

AGAACACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCC

CCCCCATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCG

ACCCCAGCAAGCCCAGCAAGAGGAGCCCCATCGAGGACCTGCTGTTCA

ACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACT

GCCTGGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTC

AACGGCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGC

CCAGTACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGA

CCTTCGGCGCCGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATG

GCCTACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAG

AACCAGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATC

CAGGACAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGA

CGTGGTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGC

TGAGCAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGA

GCAGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATC

ACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGAT

CAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGA

TGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGC

AAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGT

GGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCAC

CACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGG

AGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGG

AACTTCTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGC

GGCAACTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGAC

CCCCTGCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTA

CTTCAAGAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCG

GCATCAACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGA

ACGAGGTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAG

CTGGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTG

GGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTG

TGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGC

GGCAGCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAA

GGGCGTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCT

GTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTGCCGAGAA

CGTGACCGGCCTGTTCAAGGACTGCAGCAAGGTGATCACCGGCCTGCA

CCCCACCCAGGCCCCCACCCACCTGAGCGTGGACACCAAGTTCAAGAC

CGAGGGCCTGTGCGTGGACATCCCCGGCATCCCCAAGGACATGACCT

ACAGGAGGCTGATCAGCATGATGGGCTTCAAGATGAACTACCAGGTGA

ACGGCTACCCCAACATGTTCATCACCAGGGAGGAGGCCATCAGGCACG

TGAGGGCCTGGATCGGCTTCGACGTGGAGGGCTGCCACGCCACCAGG

GAGGCCGTGGGCACCAACCTGCCCCTGCAGCTGGGCTTCAGCACCGG

CGTGAACCTGGTGGCCGTGCCCACCGGCTACGTGGACACCCCCAACA

ACACCGACTTCAGCAGGGTGAGCGCCAAGCCCCCCCCCGGCGACCAG

TTCAAGCACCTGATCCCCCTGATGTACAAGGGCCTGCCCTGGAACGTG

GTGAGGATCAAGATCGTGCAGATGCTGAGCGACACCCTGAAGAACCTG

AGCGACAGGGTGGTGTTCGTGCTGTGGGCCCACGGCTTCGAGCTGAC

CAGCATGAAGTACTTCGTGAAGATCGGCCCCGAGAGGACCTGCTGCCT

GTGCGACAGGAGGGCCACCTGCTTCAGCACCGCCAGCGACACCTACG

CCTGCTGGCACCACAGCATCGGCTTCGACTACGTGTACAACCCCTTCA

TGATCGACGTGCAGCAGTGGGGCTTCACCGGCAACCTGCAGAGCAAC

CACGACCTGTACTGCCAGGTGCACGGCAACGCCCACGTGGCCAGCTG

CGACGCCATCATGACCAGGTGCCTGGCCGTGCACGAGTGCTTCGTGAA

GAGGGTGGACTGGACCATCGAGTACCCCATCATCGGCGACGAGCTGA

AGATCAACGCCGCCTGCAGGAAGGTGCAGCACATGGTGGTGAAGGCC

GCCCTGCTGGCCGACAAGTTCCCCGTGCTGCACGACATCGGCAACCC

CAAGGCCATCAAGTGCGTGCCCCAGGCCGACGTGGAGTGGAAGTTCTA

CGACGCCCAGCCCTGCAGCGACAAGGCCTACAAGATCGAGGAGCTGT

TCTACAGCTACGCCACCCACAGCGACAAGTTCACCGACGGCGTGTGCC

TGTTCTGGAACTGCAACGTGGACAGGTACCCCGCCAACAGCATCGTGT

GCAGGTTCGACACCAGGGTGCTGAGCAACCTGAACCTGCCCGGCTGC

GACGGCGGCAGCCTGTACGTGAACAAGCACGCCTTCCACACCCCCGC

CTTCGACAAGAGCGCCTTCGTGAACCTGAAGCAGCTGCCCTTCTTCTAC

TACAGCGACAGCCCCTGCGAGAGCCACGGCAAGCAGGTGGTGAGCGA

CATCGACTACGTGCCCCTGAAGAGCGCCACCTGCATCACCAGGTGCAA

CCTGGGCGGCGCCGTGTGCAGGCACCACGCCAACGAGTACAGGCTGT

ACCTGGACGCCTACAACATGATGATCAGCGCCGGCTTCAGCCTGTGGG

TGTACAAGCAGTTCGACACCTACAACCTGTGGAACACCTTCACCAGGCT

GCAGGGAAGCGGAGAGGGCAGGGGAAGTCTTCTAACATGCGGGGACG

TGGAGGAAAATCCCGGCCCCAGCCTGGAGAACGTGGCCTTCAACGTG

GTGAACAAGGGCCACTTCGACGGCCAGCAGGGCGAGGTGCCCGTGAG

CATCATCAACAACACCGTGTACACCAAGGTGGACGGCGTGGACGTGGA

GCTGTTCGAGAACAAGACCACCCTGCCCGTGAACGTGGCCTTCGAGCT

GTGGGCCAAGAGGAACATCAAGCCCGTGCCCGAGGTGAAGATCCTGA

ACAACCTGGGCGTGGACATCGCCGCCAACACCGTGATCTGGGACTACA

AGAGGGACGCCCCCGCCCACATCAGCACCATCGGCGTGTGCAGCATG

ACCGACATCGCCAAGAAGCCCACCGAGACCATCTGCGCCCCCCTGACC

GTGTTCTTCGACGGCAGGGTGGACGGCCAGGTGGACCTGTTCAGGAA

CGCCAGGAACGGCGTGCTGATCACCGAGGGCAGCGTGAAGGGCCTGC

AGCCCAGCGTGGGCCCCAAGCAGGCCAGCCTGAACGGCGTGACCCTG

ATCGGCGAGGCCGTGAAGACCCAGTTCAACTACTACAAGAAGGTGGAC

GGCGTGGTGCAGCAGCTGCCCGAGACCTACTTCACCCAGAGCAGGAA

CCTGCAGGAGTTCAAGCCCAGGAGCCAGATGGAGATCGACTTCCTGGA

GCTGGCCATGGACGAGTTCATCGAGAGGTACAAGCTGGAGGGCTACG

CCTTCGAGCACATCGTGTACGGCGACTTCAGCCACAGCCAGCTGGGCG

GCCTGCACCTGCTGATCGGCCTGGCCAAGAGGTTCAAGGAGAGCCCC

TTCGAGCTGGAGGACTTCATCCCCATGGACAGCACCGTGAAGAACTAC

TTCATCACCGACGCCCAGACCGGCAGCAGCAAGTGCGTGTGCAGCGT

GATCGACCTGCTGCTGGACGACTTCGTGGAGATCATCAAGAGCCAGGA

CCTGAGCGTGGTGAGCAAGGTGGTGAAGGTGACCATCGACTACACCGA

GATCAGCTTCATGCTGTGGTGCAAGGACGGCCACGTGGAGACCTTCTA

CCCCAAGCTGCAGTGATGACTCGAGCTGGTACTGCATGCACGCAATGC

TAGCTGCCCCTTTCCCGTCCTGGGTACCCCGAGTCTCCCCCGACCTCG

GGTCCCAGGTATGCTCCCACCTCCACCTGCCCCACTCACCACCTCTGC

TAGTTCCAGACACCTCCCAAGCACGCAGCAATGCAGCTCAAAACGCTTA

GCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAAT

AAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCG

TGCCAGCCACACCCTGGAGCTAGCAAAAAAAA

18
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA
303

Ad5-Pan-CoV
TGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGAC

Spike-NSP16
CGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT

AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA

CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTA

CGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATG

CCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT

ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAAT

GGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCC

ATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTC

CAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGC

GTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTC

AGATCGAGAATAAACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGA

ACCCGCCACCATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAG

CCAGTGCGTGAACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACA

CCAACAGCTTCACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGA

GCAGCGTGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCA

ACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCA

AGAGGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCG

CCAGCACCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCA

CCCTGGACAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCA

ACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCC

TGGGCGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGT

TCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCC

AGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACC

TGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAG

CAAGCACACCCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAG

CGCCCTGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAG

GTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCG

ACAGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGC

TACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACC

ATCACCGACGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAA

GTGCACCCTGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAG

CAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACAT

CACCAACCTGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGC

CAGCGTGTACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCG

ACTACAGCGTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCT

ACGGCGTGAGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGT

ACGCCGACAGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCC

CCCGGCCAGACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGA

CGACTTCACCGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAG

CAAGGTGGGCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAG

CAACCTGAAGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGC

CGGCAGCACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCC

CCTGCAGAGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGC

CCTACAGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCC

ACCGTGTGCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGC

GTGAACTTCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAG

AGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCC

GACACCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGA

CATCACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCA

CCAACACCAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCA

CCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGG

AGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTG

CCTGATCGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCC

CATCGGCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCC

CCAGGAGGGCCAGGAGCGTGGCCAGCCAGAGCATCATCGCCTACACC

ATGAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCAT

CGCCATCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCC

CGTGAGCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGG

CGACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTG

CACCCAGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACA

AGAACACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCC

CCCCCATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCG

ACCCCAGCAAGCCCAGCAAGAGGAGCTTCATCGAGGACCTGCTGTTCA

ACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACT

GCCTGGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTC

AACGGCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGC

CCAGTACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGA

CCTTCGGCGCCGGCGCCGCCCTGCAGATCCCCTTCGCCATGCAGATG

GCCTACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAG

AACCAGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATC

CAGGACAGCCTGAGCAGCACCGCCAGCGCCCTGGGCAAGCTGCAGGA

CGTGGTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGC

TGAGCAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGA

GCAGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATC

ACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGAT

CAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGA

TGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGC

AAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGT

GGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCAC

CACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGG

AGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGG

AACTTCTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGC

GGCAACTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGAC

CCCCTGCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTA

CTTCAAGAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCG

GCATCAACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGA

ACGAGGTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAG

CTGGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTG

GGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTG

TGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGC

GGCAGCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAA

GGGCGTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCT

GTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTAGCAGCCA

GGCCTGGCAGCCCGGCGTGGCCATGCCCAACCTGTACAAGATGCAGA

GGATGCTGCTGGAGAAGTGCGACCTGCAGAACTACGGCGACAGCGCC

ACCCTGCCCAAGGGCATCATGATGAACGTGGCCAAGTACACCCAGCTG

TGCCAGTACCTGAACACCCTGACCCTGGCCGTGCCCTACAACATGAGG

GTGATCCACTTCGGCGCCGGCAGCGACAAGGGCGTGGCCCCCGGCAC

CGCCGTGCTGAGGCAGTGGCTGCCCACCGGCACCCTGCTGGTGGACA

GCGACCTGAACGACTTCGTGAGCGACGCCGACAGCACCCTGATCGGC

GACTGCGCCACCGTGCACACCGCCAACAAGTGGGACCTGATCATCAGC

GACATGTACGACCCCAAGACCAAGAACGTGACCAAGGAGAACGACAGC

AAGGAGGGCTTCTTCACCTACATCTGCGGCTTCATCCAGCAGAAGCTG

GCCCTGGGCGGCAGCGTGGCCATCAAGATCACCGAGCACAGCTGGAA

CGCCGACCTGTACAAGCTGATGGGCCACTTCGCCTGGTGGACCGCCTT

CGTGACCAACGTGAACGCCAGCAGCAGCGAGGCCTTCCTGATCGGCT

GCAACTACCTGGGCAAGCCCAGGGAGCAGATCGACGGCTACGTGATG

CACGCCAACTACATCTTCTGGAGGAACACCAACCCCATCCAGCTGAGC

AGCTACAGCCTGTTCGACATGAGCAAGTTCCCCCTGAAGCTGAGGGGC

ACCGCCGTGATGAGCCTGAAGGAGGGCCAGATCAACGACATGATCCTG

AGCCTGCTGAGCAAGGGCAGGCTGATCATCAGGGAGAACAACAGGGT

GGTGATCAGCAGCGACGTGCTGGTGAACAACTGATGACTCGAGCTGGT

ACTGCATGCACGCAATGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCC

GAGTCTCCCCCGACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGC

CCCACTCACCACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCA

ATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCA

GTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCC

CAGGGTTGGTCAATTTCGTGCCAGCCACACCCTGGAGCTAGCAAAAAA

AA

19
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA
304

Ad5-Pan-CoV
TGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGAC

HexaPro-
CGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT

NSP16
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA

CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTA

CGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATG

CCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT

ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAAT

GGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCC

ATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTC

CAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGC

GTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTC

AGATCGAGAATAAACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGA

ACCCGCCACCATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAG

CCAGTGCGTGAACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACA

CCAACAGCTTCACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGA

GCAGCGTGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCA

ACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCA

AGAGGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCG

CCAGCACCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCA

CCCTGGACAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCA

ACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCC

TGGGCGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGT

TCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCC

AGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACC

TGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAG

CAAGCACACCCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAG

CGCCCTGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAG

GTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCG

ACAGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGC

TACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACC

ATCACCGACGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAA

GTGCACCCTGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAG

CAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACAT

CACCAACCTGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGC

CAGCGTGTACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCG

ACTACAGCGTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCT

ACGGCGTGAGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGT

ACGCCGACAGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCC

CCCGGCCAGACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGA

CGACTTCACCGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAG

CAAGGTGGGCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAG

CAACCTGAAGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGC

CGGCAGCACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCC

CCTGCAGAGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGC

CCTACAGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCC

ACCGTGTGCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGC

GTGAACTTCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAG

AGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCC

GACACCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGA

CATCACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCA

CCAACACCAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCA

CCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGG

AGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTG

CCTGATCGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCC

CATCGGCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCC

CCGGCAGCGCCAGCAGCGTGGCCAGCCAGAGCATCATCGCCTACACC

ATGAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCAT

CGCCATCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCC

CGTGAGCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGG

CGACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTG

CACCCAGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACA

AGAACACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCC

CCCCCATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCG

ACCCCAGCAAGCCCAGCAAGAGGAGCCCCATCGAGGACCTGCTGTTCA

ACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACT

GCCTGGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTC

AACGGCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGC

CCAGTACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGA

CCTTCGGCGCCGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATG

GCCTACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAG

AACCAGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATC

CAGGACAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGA

CGTGGTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGC

TGAGCAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGA

GCAGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATC

ACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGAT

CAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGA

TGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGC

AAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGT

GGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCAC

CACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGG

AGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGG

AACTTCTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGC

GGCAACTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGAC

CCCCTGCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTA

CTTCAAGAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCG

GCATCAACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGA

ACGAGGTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAG

CTGGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTG

GGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTG

TGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGC

GGCAGCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAA

GGGCGTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCT

GTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTAGCAGCCA

GGCCTGGCAGCCCGGCGTGGCCATGCCCAACCTGTACAAGATGCAGA

GGATGCTGCTGGAGAAGTGCGACCTGCAGAACTACGGCGACAGCGCC

ACCCTGCCCAAGGGCATCATGATGAACGTGGCCAAGTACACCCAGCTG

TGCCAGTACCTGAACACCCTGACCCTGGCCGTGCCCTACAACATGAGG

GTGATCCACTTCGGCGCCGGCAGCGACAAGGGCGTGGCCCCCGGCAC

CGCCGTGCTGAGGCAGTGGCTGCCCACCGGCACCCTGCTGGTGGACA

GCGACCTGAACGACTTCGTGAGCGACGCCGACAGCACCCTGATCGGC

GACTGCGCCACCGTGCACACCGCCAACAAGTGGGACCTGATCATCAGC

GACATGTACGACCCCAAGACCAAGAACGTGACCAAGGAGAACGACAGC

AAGGAGGGCTTCTTCACCTACATCTGCGGCTTCATCCAGCAGAAGCTG

GCCCTGGGCGGCAGCGTGGCCATCAAGATCACCGAGCACAGCTGGAA

CGCCGACCTGTACAAGCTGATGGGCCACTTCGCCTGGTGGACCGCCTT

CGTGACCAACGTGAACGCCAGCAGCAGCGAGGCCTTCCTGATCGGCT

GCAACTACCTGGGCAAGCCCAGGGAGCAGATCGACGGCTACGTGATG

CACGCCAACTACATCTTCTGGAGGAACACCAACCCCATCCAGCTGAGC

AGCTACAGCCTGTTCGACATGAGCAAGTTCCCCCTGAAGCTGAGGGGC

ACCGCCGTGATGAGCCTGAAGGAGGGCCAGATCAACGACATGATCCTG

AGCCTGCTGAGCAAGGGCAGGCTGATCATCAGGGAGAACAACAGGGT

GGTGATCAGCAGCGACGTGCTGGTGAACAACTGATGACTCGAGCTGGT

ACTGCATGCACGCAATGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCC

GAGTCTCCCCCGACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGC

CCCACTCACCACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCA

ATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCA

GTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCC

CAGGGTTGGTCAATTTCGTGCCAGCCACACCCTGGAGCTAGCAAAAAA

AA

20
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA
305

Ad5-Pan-CoV
TGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGAC

Spike-ORF3a
CGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT

AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA

CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTA

CGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATG

CCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT

ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAAT

GGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCC

ATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTC

CAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGC

GTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTC

AGATCGAGAATAAACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGA

ACCCGCCACCATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAG

CCAGTGCGTGAACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACA

CCAACAGCTTCACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGA

GCAGCGTGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCA

ACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCA

AGAGGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCG

CCAGCACCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCA

CCCTGGACAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCA

ACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCC

TGGGCGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGT

TCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCC

AGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACC

TGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAG

CAAGCACACCCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAG

CGCCCTGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAG

GTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCG

ACAGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGC

TACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACC

ATCACCGACGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAA

GTGCACCCTGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAG

CAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACAT

CACCAACCTGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGC

CAGCGTGTACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCG

ACTACAGCGTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCT

ACGGCGTGAGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGT

ACGCCGACAGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCC

CCCGGCCAGACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGA

CGACTTCACCGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAG

CAAGGTGGGCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAG

CAACCTGAAGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGC

CGGCAGCACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCC

CCTGCAGAGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGC

CCTACAGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCC

ACCGTGTGCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGC

GTGAACTTCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAG

AGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCC

GACACCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGA

CATCACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCA

CCAACACCAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCA

CCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGG

AGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTG

CCTGATCGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCC

CATCGGCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCC

CCAGGAGGGCCAGGAGCGTGGCCAGCCAGAGCATCATCGCCTACACC

ATGAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCAT

CGCCATCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCC

CGTGAGCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGG

CGACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTG

CACCCAGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACA

AGAACACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCC

CCCCCATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCG

ACCCCAGCAAGCCCAGCAAGAGGAGCTTCATCGAGGACCTGCTGTTCA

ACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACT

GCCTGGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTC

AACGGCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGC

CCAGTACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGA

CCTTCGGCGCCGGCGCCGCCCTGCAGATCCCCTTCGCCATGCAGATG

GCCTACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAG

AACCAGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATC

CAGGACAGCCTGAGCAGCACCGCCAGCGCCCTGGGCAAGCTGCAGGA

CGTGGTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGC

TGAGCAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGA

GCAGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATC

ACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGAT

CAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGA

TGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGC

AAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGT

GGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCAC

CACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGG

AGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGG

AACTTCTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGC

GGCAACTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGAC

CCCCTGCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTA

CTTCAAGAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCG

GCATCAACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGA

ACGAGGTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAG

CTGGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTG

GGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTG

TGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGC

GGCAGCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAA

GGGCGTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCT

GTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTATGGACCT

GTTCATGAGGATCTTCACCATCGGCACCGTGACCCTGAAGCAGGGCGA

GATCAAGGACGCCACCCCCAGCGACTTCGTGAGGGCCACCGCCACCA

TCCCCATCCAGGCCAGCCTGCCCTTCGGCTGGCTGATCGTGGGCGTG

GCCCTGCTGGCCGTGTTCCAGAGCGCCAGCAAGATCATCACCCTGAAG

AAGAGGTGGCAGCTGGCCCTGAGCAAGGGCGTGCACTTCGTGTGCAA

CCTGCTGCTGCTGTTCGTGACCGTGTACAGCCACCTGCTGCTGGTGGC

CGCCGGCCTGGAGGCCCCCTTCCTGTACCTGTACGCCCTGGTGTACTT

CCTGCAGAGCATCAACTTCGTGAGGATCATCATGAGGCTGTGGCTGTG

CTGGAAGTGCAGGAGCAAGAACCCCCTGCTGTACGACGCCAACTACTT

CCTGTGCTGGCACACCAACTGCTACGACTACTGCATCCCCTACAACAG

CGTGACCAGCAGCATCGTGATCACCAGCGGCGACGGCACCACCAGCC

CCATCAGCGAGCACGACTACCAGATCGGCGGCTACACCGAGAAGTGG

GAGAGCGGCGTGAAGGACTGCGTGGTGCTGCACAGCTACTTCACCAG

CGACTACTACCAGCTGTACAGCACCCAGCTGAGCACCGACACCGGCGT

GGAGCACGTGACCTTCTTCATCTACAACAAGATCGTGGACGAGCCCGA

GGAGCACGTGCAGATCCACACCATCGACGGCAGCAGCGGCGTGGTGA

ACCCCGTGATGGAGCCCATCTACGACGAGCCCACCACCACCACCAGC

GTGCCCCTGTGATGACTCGAGCTGGTACTGCATGCACGCAATGCTAGC

TGCCCCTTTCCCGTCCTGGGTACCCCGAGTCTCCCCCGACCTCGGGTC

CCAGGTATGCTCCCACCTCCACCTGCCCCACTCACCACCTCTGCTAGTT

CCAGACACCTCCCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCT

AGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAAC

GAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCC

AGCCACACCCTGGAGCTAGCAAAAAAAA

21
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA
306

Ad5-Pan-CoV
TGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGAC

HexaPro-
CGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT

ORF3a
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA

CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTA

CGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATG

CCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT

ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAAT

GGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCC

ATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTC

CAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGC

GTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTC

AGATCGAGAATAAACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGA

ACCCGCCACCATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAG

CCAGTGCGTGAACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACA

CCAACAGCTTCACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGA

GCAGCGTGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCA

ACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCA

AGAGGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCG

CCAGCACCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCA

CCCTGGACAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCA

ACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCC

TGGGCGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGT

TCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCC

AGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACC

TGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAG

CAAGCACACCCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAG

CGCCCTGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAG

GTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCG

ACAGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGC

TACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACC

ATCACCGACGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAA

GTGCACCCTGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAG

CAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACAT

CACCAACCTGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGC

CAGCGTGTACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCG

ACTACAGCGTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCT

ACGGCGTGAGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGT

ACGCCGACAGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCC

CCCGGCCAGACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGA

CGACTTCACCGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAG

CAAGGTGGGCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAG

CAACCTGAAGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGC

CGGCAGCACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCC

CCTGCAGAGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGC

CCTACAGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCC

ACCGTGTGCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGC

GTGAACTTCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAG

AGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCC

GACACCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGA

CATCACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCA

CCAACACCAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCA

CCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGG

AGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTG

CCTGATCGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCC

CATCGGCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCC

CCGGCAGCGCCAGCAGCGTGGCCAGCCAGAGCATCATCGCCTACACC

ATGAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCAT

CGCCATCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCC

CGTGAGCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGG

CGACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTG

CACCCAGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACA

AGAACACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCC

CCCCCATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCG

ACCCCAGCAAGCCCAGCAAGAGGAGCCCCATCGAGGACCTGCTGTTCA

ACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACT

GCCTGGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTC

AACGGCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGC

CCAGTACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGA

CCTTCGGCGCCGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATG

GCCTACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAG

AACCAGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATC

CAGGACAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGA

CGTGGTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGC

TGAGCAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGA

GCAGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATC

ACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGAT

CAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGA

TGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGC

AAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGT

GGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCAC

CACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGG

AGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGG

AACTTCTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGC

GGCAACTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGAC

CCCCTGCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTA

CTTCAAGAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCG

GCATCAACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGA

ACGAGGTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAG

CTGGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTG

GGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTG

TGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGC

GGCAGCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAA

GGGCGTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCT

GTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTATGGACCT

GTTCATGAGGATCTTCACCATCGGCACCGTGACCCTGAAGCAGGGCGA

GATCAAGGACGCCACCCCCAGCGACTTCGTGAGGGCCACCGCCACCA

TCCCCATCCAGGCCAGCCTGCCCTTCGGCTGGCTGATCGTGGGCGTG

GCCCTGCTGGCCGTGTTCCAGAGCGCCAGCAAGATCATCACCCTGAAG

AAGAGGTGGCAGCTGGCCCTGAGCAAGGGCGTGCACTTCGTGTGCAA

CCTGCTGCTGCTGTTCGTGACCGTGTACAGCCACCTGCTGCTGGTGGC

CGCCGGCCTGGAGGCCCCCTTCCTGTACCTGTACGCCCTGGTGTACTT

CCTGCAGAGCATCAACTTCGTGAGGATCATCATGAGGCTGTGGCTGTG

CTGGAAGTGCAGGAGCAAGAACCCCCTGCTGTACGACGCCAACTACTT

CCTGTGCTGGCACACCAACTGCTACGACTACTGCATCCCCTACAACAG

CGTGACCAGCAGCATCGTGATCACCAGCGGCGACGGCACCACCAGCC

CCATCAGCGAGCACGACTACCAGATCGGCGGCTACACCGAGAAGTGG

GAGAGCGGCGTGAAGGACTGCGTGGTGCTGCACAGCTACTTCACCAG

CGACTACTACCAGCTGTACAGCACCCAGCTGAGCACCGACACCGGCGT

GGAGCACGTGACCTTCTTCATCTACAACAAGATCGTGGACGAGCCCGA

GGAGCACGTGCAGATCCACACCATCGACGGCAGCAGCGGCGTGGTGA

ACCCCGTGATGGAGCCCATCTACGACGAGCCCACCACCACCACCAGC

GTGCCCCTGTGATGACTCGAGCTGGTACTGCATGCACGCAATGCTAGC

TGCCCCTTTCCCGTCCTGGGTACCCCGAGTCTCCCCCGACCTCGGGTC

CCAGGTATGCTCCCACCTCCACCTGCCCCACTCACCACCTCTGCTAGTT

CCAGACACCTCCCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCT

AGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAAC

GAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCC

AGCCACACCCTGGAGCTAGCAAAAAAAA

22
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGTG
307

Pan-CoV
AACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAGCTTC

mRNA Spike-
ACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCT

NSP1-NSP2
GCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTG

GTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGA

CAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAG

CAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGAT

CAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTA

CTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTA

CAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCT

GATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGT

TCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACAC

CCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCCTGG

AGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGA

CCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGC

AGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCA

GCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGA

CGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGCACCC

TGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCA

GGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACC

TGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGT

ACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGC

GTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTG

AGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGAC

AGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCA

GACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCAC

CGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGG

GCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAGC

ACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTGCAG

AGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCTACAG

GGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGT

GCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACT

TCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACA

AGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCA

CCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACC

CCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACAC

CAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGG

TGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTG

TACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGAT

CGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCG

GCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCAGG

AGGGCCAGGAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAG

CCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCA

TCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGA

GCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACA

GCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCACCC

AGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACAAGAAC

ACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCCCCC

ATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCGACCCC

AGCAAGCCCAGCAAGAGGAGCTTCATCGAGGACCTGCTGTTCAACAAG

GTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCT

GGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACG

GCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAG

TACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTT

CGGCGCCGGCGCCGCCCTGCAGATCCCCTTCGCCATGCAGATGGCCT

ACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACC

AGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGG

ACAGCCTGAGCAGCACCGCCAGCGCCCTGGGCAAGCTGCAGGACGTG

GTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAG

CAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAG

GCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCG

GCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGG

GCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAG

CGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGG

GCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTG

TTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACC

GCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGG

CGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTT

CTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAA

CTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCT

GCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCA

ACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAG

GTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGG

CAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTT

CATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTG

CATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGGCA

GCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGC

GTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCTGTTA

AAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTATGGAGAGCCTG

GTGCCCGGCTTCAACGAGAAGACCCACGTGCAGCTGAGCCTGCCCGT

GCTGCAGGTGAGGGACGTGCTGGTGAGGGGCTTCGGCGACAGCGTGG

AGGAGGTGCTGAGCGAGGCCAGGCAGCACCTGAAGGACGGCACCTGC

GGCCTGGTGGAGGTGGAGAAGGGCGTGCTGCCCCAGCTGGAGCAGC

CCTACGTGTTCATCAAGAGGAGCGACGCCAGGACCGCCCCCCACGGC

CACGTGATGGTGGAGCTGGTGGCCGAGCTGGAGGGCATCCAGTACGG

CAGGAGCGGCGAGACCCTGGGCGTGCTGGTGCCCCACGTGGGCGAG

ATCCCCGTGGCCTACAGGAAGGTGCTGCTGAGGAAGAACGGCAACAA

GGGCGCCGGCGGCCACAGCTACGGCGCCGACCTGAAGAGCTTCGACC

TGGGCGACGAGCTGGGCACCGACCCCTACGAGGACTTCCAGGAGAAC

TGGAACACCAAGCACAGCAGCGGCGTGACCAGGGAGCTGATGAGGGA

GCTGAACGGCGGCGGAAGCGGAGAGGGCAGGGGAAGTCTTCTAACAT

GCGGGGACGTGGAGGAAAATCCCGGCCCCGCCTACACCAGGTACGTG

GACAACAACTTCTGCGGCCCCGACGGCTACCCCCTGGAGTGCATCAAG

GACCTGCTGGCCAGGGCCGGCAAGGCCAGCTGCACCCTGAGCGAGCA

GCTGGACTTCATCGACACCAAGAGGGGCGTGTACTGCTGCAGGGAGC

ACGAGCACGAGATCGCCTGGTACACCGAGAGGAGCGAGAAGAGCTAC

GAGCTGCAGACCCCCTTCGAGATCAAGCTGGCCAAGAAGTTCGACACC

TTCAACGGCGAGTGCCCCAACTTCGTGTTCCCCCTGAACAGCATCATCA

AGACCATCCAGCCCAGGGTGGAGAAGAAGAAGCTGGACGGCTTCATG

GGCAGGATCAGGAGCGTGTACCCCGTGGCCAGCCCCAACGAGTGCAA

CCAGATGTGCCTGAGCACCCTGATGAAGTGCGACCACTGCGGCGAGA

CCAGCTGGCAGACCGGCGACTTCGTGAAGGCCACCTGCGAGTTCTGC

GGCACCGAGAACCTGACCAAGGAGGGCGCCACCACCTGCGGCTACCT

GCCCCAGAACGCCGTGGTGAAGATCTACTGCCCCGCCTGCCACAACAG

CGAGGTGGGCCCCGAGCACAGCCTGGCCGAGTACCACAACGAGAGCG

GCCTGAAGACCATCCTGAGGAAGGGCGGCAGGACCATCGCCTTCGGC

GGCTGCGTGTTCAGCTACGTGGGCTGCCACAACAAGTGCGCCTACTGG

GTGCCCAGGGCCAGCGCCAACATCGGCTGCAACCACACCGGCGTGGT

GGGCGAGGGCAGCGAGGGCCTGAACGACAACCTGCTGGAGATCCTGC

AGAAGGAGAAGGTGAACATCAACATCGTGGGCGACTTCAAGCTGAACG

AGGAGATCGCCATCATCCTGGCCAGCTTCAGCGCCAGCACCAGCGCCT

TCGTGGAGACCGTGAAGGGCCTGGACTACAAGGCCTTCAAGCAGATCG

TGGAGAGCTGCGGCAACTTCAAGGTGACCAAGGGCAAGGCCAAGAAG

GGCGCCTGGAACATCGGCGAGCAGAAGAGCATCCTGAGCCCCCTGTA

CGCCTTCGCCAGCGAGGCCGCCAGGGTGGTGAGGAGCATCTTCAGCA

GGACCCTGGAGACCGCCCAGAACAGCGTGAGGGTGCTGCAGAAGGCC

GCCATCACCATCCTGGACGGCATCAGCCAGTACAGCCTGAGGCTGATC

GACGCCATGATGTTCACCAGCGACCTGGCCACCAACAACCTGGTGGTG

ATGGCCTACATCACCGGCGGCGTGGTGCAGCTGACCAGCCAGTGGCT

GACCAACATCTTCGGCACCGTGTACGAGAAGCTGAAGCCCGTGCTGGA

CTGGCTGGAGGAGAAGTTCAAGGAGGGCGTGGAGTTCCTGAGGGACG

GCTGGGAGATCGTGAAGTTCATCAGCACCTGCGCCTGCGAGATCGTGG

GCGGCCAGATCGTGACCTGCGCCAAGGAGATCAAGGAGAGCGTGCAG

ACCTTCTTCAAGCTGGTGAACAAGTTCCTGGCCCTGTGCGCCGACAGC

ATCATCATCGGCGGCGCCAAGCTGAAGGCCCTGAACCTGGGCGAGAC

CTTCGTGACCCACAGCAAGGGCCTGTACAGGAAGTGCGTGAAGAGCAG

GGAGGAGACCGGCCTGCTGATGCCCCTGAAGGCCCCCAAGGAGATCA

TCTTCCTGGAGGGCGAGACCCTGCCCACCGAGGTGCTGACCGAGGAG

GTGGTGCTGAAGACCGGCGACCTGCAGCCCCTGGAGCAGCCCACCAG

CGAGGCCGTGGAGGCCCCCCTGGTGGGCACCCCCGTGTGCATCAACG

GCCTGATGCTGCTGGAGATCAAGGACACCGAGAAGTACTGCGCCCTGG

CCCCCAACATGATGGTGACCAACAACACCTTCACCCTGAAGGGCGGCT

GATGA

23
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGTG
308

Pan-CoV
AACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAGCTTC

mRNA
ACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCT

HexaPro-
GCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTG

NSP1-NSP2
GTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGA

CAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAG

CAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGAT

CAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTA

CTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTA

CAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCT

GATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGT

TCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACAC

CCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCCTGG

AGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGA

CCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGC

AGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCA

GCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGA

CGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGCACCC

TGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCA

GGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACC

TGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGT

ACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGC

GTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTG

AGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGAC

AGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCA

GACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCAC

CGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGG

GCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAGC

ACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTGCAG

AGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCTACAG

GGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGT

GCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACT

TCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACA

AGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCA

CCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACC

CCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACAC

CAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGG

TGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTG

TACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGAT

CGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCG

GCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCGGC

AGCGCCAGCAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAG

CCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCA

TCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGA

GCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACA

GCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCACCC

AGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACAAGAAC

ACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCCCCC

ATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCGACCCC

AGCAAGCCCAGCAAGAGGAGCCCCATCGAGGACCTGCTGTTCAACAAG

GTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCT

GGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACG

GCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAG

TACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTT

CGGCGCCGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATGGCCT

ACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACC

AGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGG

ACAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGTG

GTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAG

CAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAG

GCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCG

GCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGG

GCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAG

CGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGG

GCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTG

TTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACC

GCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGG

CGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTT

CTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAA

CTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCT

GCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCA

ACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAG

GTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGG

CAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTT

CATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTG

CATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGGCA

GCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGC

GTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCTGTTA

AAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTATGGAGAGCCTG

GTGCCCGGCTTCAACGAGAAGACCCACGTGCAGCTGAGCCTGCCCGT

GCTGCAGGTGAGGGACGTGCTGGTGAGGGGCTTCGGCGACAGCGTGG

AGGAGGTGCTGAGCGAGGCCAGGCAGCACCTGAAGGACGGCACCTGC

GGCCTGGTGGAGGTGGAGAAGGGCGTGCTGCCCCAGCTGGAGCAGC

CCTACGTGTTCATCAAGAGGAGCGACGCCAGGACCGCCCCCCACGGC

CACGTGATGGTGGAGCTGGTGGCCGAGCTGGAGGGCATCCAGTACGG

CAGGAGCGGCGAGACCCTGGGCGTGCTGGTGCCCCACGTGGGCGAG

ATCCCCGTGGCCTACAGGAAGGTGCTGCTGAGGAAGAACGGCAACAA

GGGCGCCGGCGGCCACAGCTACGGCGCCGACCTGAAGAGCTTCGACC

TGGGCGACGAGCTGGGCACCGACCCCTACGAGGACTTCCAGGAGAAC

TGGAACACCAAGCACAGCAGCGGCGTGACCAGGGAGCTGATGAGGGA

GCTGAACGGCGGCGGAAGCGGAGAGGGCAGGGGAAGTCTTCTAACAT

GCGGGGACGTGGAGGAAAATCCCGGCCCCGCCTACACCAGGTACGTG

GACAACAACTTCTGCGGCCCCGACGGCTACCCCCTGGAGTGCATCAAG

GACCTGCTGGCCAGGGCCGGCAAGGCCAGCTGCACCCTGAGCGAGCA

GCTGGACTTCATCGACACCAAGAGGGGCGTGTACTGCTGCAGGGAGC

ACGAGCACGAGATCGCCTGGTACACCGAGAGGAGCGAGAAGAGCTAC

GAGCTGCAGACCCCCTTCGAGATCAAGCTGGCCAAGAAGTTCGACACC

TTCAACGGCGAGTGCCCCAACTTCGTGTTCCCCCTGAACAGCATCATCA

AGACCATCCAGCCCAGGGTGGAGAAGAAGAAGCTGGACGGCTTCATG

GGCAGGATCAGGAGCGTGTACCCCGTGGCCAGCCCCAACGAGTGCAA

CCAGATGTGCCTGAGCACCCTGATGAAGTGCGACCACTGCGGCGAGA

CCAGCTGGCAGACCGGCGACTTCGTGAAGGCCACCTGCGAGTTCTGC

GGCACCGAGAACCTGACCAAGGAGGGCGCCACCACCTGCGGCTACCT

GCCCCAGAACGCCGTGGTGAAGATCTACTGCCCCGCCTGCCACAACAG

CGAGGTGGGCCCCGAGCACAGCCTGGCCGAGTACCACAACGAGAGCG

GCCTGAAGACCATCCTGAGGAAGGGCGGCAGGACCATCGCCTTCGGC

GGCTGCGTGTTCAGCTACGTGGGCTGCCACAACAAGTGCGCCTACTGG

GTGCCCAGGGCCAGCGCCAACATCGGCTGCAACCACACCGGCGTGGT

GGGCGAGGGCAGCGAGGGCCTGAACGACAACCTGCTGGAGATCCTGC

AGAAGGAGAAGGTGAACATCAACATCGTGGGCGACTTCAAGCTGAACG

AGGAGATCGCCATCATCCTGGCCAGCTTCAGCGCCAGCACCAGCGCCT

TCGTGGAGACCGTGAAGGGCCTGGACTACAAGGCCTTCAAGCAGATCG

TGGAGAGCTGCGGCAACTTCAAGGTGACCAAGGGCAAGGCCAAGAAG

GGCGCCTGGAACATCGGCGAGCAGAAGAGCATCCTGAGCCCCCTGTA

CGCCTTCGCCAGCGAGGCCGCCAGGGTGGTGAGGAGCATCTTCAGCA

GGACCCTGGAGACCGCCCAGAACAGCGTGAGGGTGCTGCAGAAGGCC

GCCATCACCATCCTGGACGGCATCAGCCAGTACAGCCTGAGGCTGATC

GACGCCATGATGTTCACCAGCGACCTGGCCACCAACAACCTGGTGGTG

ATGGCCTACATCACCGGCGGCGTGGTGCAGCTGACCAGCCAGTGGCT

GACCAACATCTTCGGCACCGTGTACGAGAAGCTGAAGCCCGTGCTGGA

CTGGCTGGAGGAGAAGTTCAAGGAGGGCGTGGAGTTCCTGAGGGACG

GCTGGGAGATCGTGAAGTTCATCAGCACCTGCGCCTGCGAGATCGTGG

GCGGCCAGATCGTGACCTGCGCCAAGGAGATCAAGGAGAGCGTGCAG

ACCTTCTTCAAGCTGGTGAACAAGTTCCTGGCCCTGTGCGCCGACAGC

ATCATCATCGGCGGCGCCAAGCTGAAGGCCCTGAACCTGGGCGAGAC

CTTCGTGACCCACAGCAAGGGCCTGTACAGGAAGTGCGTGAAGAGCAG

GGAGGAGACCGGCCTGCTGATGCCCCTGAAGGCCCCCAAGGAGATCA

TCTTCCTGGAGGGCGAGACCCTGCCCACCGAGGTGCTGACCGAGGAG

GTGGTGCTGAAGACCGGCGACCTGCAGCCCCTGGAGCAGCCCACCAG

CGAGGCCGTGGAGGCCCCCCTGGTGGGCACCCCCGTGTGCATCAACG

GCCTGATGCTGCTGGAGATCAAGGACACCGAGAAGTACTGCGCCCTGG

CCCCCAACATGATGGTGACCAACAACACCTTCACCCTGAAGGGCGGCT

GATGA

24
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGTG
309

Pan-CoV
AACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAGCTTC

mRNA Spike-
ACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCT

NSP6-NSP7-
GCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTG

NSP8
GTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGA

CAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAG

CAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGAT

CAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTA

CTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTA

CAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCT

GATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGT

TCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACAC

CCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCCTGG

AGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGA

CCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGC

AGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCA

GCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGA

CGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGCACCC

TGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCA

GGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACC

TGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGT

ACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGC

GTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTG

AGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGAC

AGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCA

GACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCAC

CGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGG

GCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAGC

ACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTGCAG

AGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCTACAG

GGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGT

GCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACT

TCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACA

AGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCA

CCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACC

CCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACAC

CAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGG

TGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTG

TACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGAT

CGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCG

GCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCAGG

AGGGCCAGGAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAG

CCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCA

TCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGA

GCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACA

GCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCACCC

AGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACAAGAAC

ACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCCCCC

ATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCGACCCC

AGCAAGCCCAGCAAGAGGAGCTTCATCGAGGACCTGCTGTTCAACAAG

GTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCT

GGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACG

GCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAG

TACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTT

CGGCGCCGGCGCCGCCCTGCAGATCCCCTTCGCCATGCAGATGGCCT

ACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACC

AGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGG

ACAGCCTGAGCAGCACCGCCAGCGCCCTGGGCAAGCTGCAGGACGTG

GTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAG

CAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAG

GCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCG

GCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGG

GCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAG

CGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGG

GCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTG

TTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACC

GCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGG

CGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTT

CTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAA

CTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCT

GCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCA

ACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAG

GTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGG

CAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTT

CATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTG

CATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGGCA

GCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGC

GTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCTGTTA

AAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTAGCGCCGTGAA

GAGGACCATCAAGGGCACCCACCACTGGCTGCTGCTGACCATCCTGAC

CAGCCTGCTGGTGCTGGTGCAGAGCACCCAGTGGAGCCTGTTCTTCTT

CCTGTACGAGAACGCCTTCCTGCCCTTCGCCATGGGCATCATCGCCAT

GAGCGCCTTCGCCATGATGTTCGTGAAGCACAAGCACGCCTTCCTGTG

CCTGTTCCTGCTGCCCAGCCTGGCCACCGTGGCCTACTTCAACATGGT

GTACATGCCCGCCAGCTGGGTGATGAGGATCATGACCTGGCTGGACAT

GGTGGACACCAGCCTGAGCGGCTTCAAGCTGAAGGACTGCGTGATGTA

CGCCAGCGCCGTGGTGCTGCTGATCCTGATGACCGCCAGGACCGTGT

ACGACGACGGCGCCAGGAGGGTGTGGACCCTGATGAACGTGCTGACC

CTGGTGTACAAGGTGTACTACGGCAACGCCCTGGACCAGGCCATCAGC

ATGTGGGCCCTGATCATCAGCGTGACCAGCAACTACAGCGGCGTGGTG

ACCACCGTGATGTTCCTGGCCAGGGGCATCGTGTTCATGTGCGTGGAG

TACTGCCCCATCTTCTTCATCACCGGCAACACCCTGCAGTGCATCATGC

TGGTGTACTGCTTCCTGGGCTACTTCTGCACCTGCTACTTCGGCCTGTT

CTGCCTGCTGAACAGGTACTTCAGGCTGACCCTGGGCGTGTACGACTA

CCTGGTGAGCACCCAGGAGTTCAGGTACATGAACAGCCAGGGCCTGCT

GCCCCCCAAGAACAGCATCGACGCCTTCAAGCTGAACATCAAGCTGCT

GGGCGTGGGCGGCAAGCCCTGCATCAAGGTGGCCACCGTGCAGGGAA

GCGGAGAGGGCAGGGGAAGTCTTCTAACATGCGGGGACGTGGAGGAA

AATCCCGGCCCCAGCAAGATGAGCGACGTGAAGTGCACCAGCGTGGT

GCTGCTGAGCGTGCTGCAGCAGCTGAGGGTGGAGAGCAGCAGCAAGC

TGTGGGCCCAGTGCGTGCAGCTGCACAACGACATCCTGCTGGCCAAG

GACACCACCGAGGCCTTCGAGAAGATGGTGAGCCTGCTGAGCGTGCT

GCTGAGCATGCAGGGCGCCGTGGACATCAACAAGCTGTGCGAGGAGA

TGCTGGACAACAGGGCCACCCTGCAGGGAAGCGGACAGTGTACTAATT

ATGCTCTCTTGAAATTGGCTGGAGATGTTGAGAGCAACCCAGGTCCCG

CCATCGCCAGCGAGTTCAGCAGCCTGCCCAGCTACGCCGCCTTCGCCA

CCGCCCAGGAGGCCTACGAGCAGGCCGTGGCCAACGGCGACAGCGA

GGTGGTGCTGAAGAAGCTGAAGAAGAGCCTGAACGTGGCCAAGAGCG

AGTTCGACAGGGACGCCGCCATGCAGAGGAAGCTGGAGAAGATGGCC

GACCAGGCCATGACCCAGATGTACAAGCAGGCCAGGAGCGAGGACAA

GAGGGCCAAGGTGACCAGCGCCATGCAGACCATGCTGTTCACCATGCT

GAGGAAGCTGGACAACGACGCCCTGAACAACATCATCAACAACGCCAG

GGACGGCTGCGTGCCCCTGAACATCATCCCCCTGACCACCGCCGCCA

AGCTGATGGTGGTGATCCCCGACTACAACACCTACAAGAACACCTGCG

ACGGCACCACCTTCACCTACGCCAGCGCCCTGTGGGAGATCCAGCAG

GTGGTGGACGCCGACAGCAAGATCGTGCAGCTGAGCGAGATCAGCAT

GGACAACAGCCCCAACCTGGCCTGGCCCCTGATCGTGACCGCCCTGA

GGGCCAACAGCGCCGTGAAGCTGCAGTGATGA

25
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGTG
310

Pan-CoV
AACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAGCTTC

mRNA
ACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCT

HexaPro-
GCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTG

NSP6-NSP7-
GTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGA

NSP8
CAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAG

CAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGAT

CAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTA

CTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTA

CAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCT

GATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGT

TCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACAC

CCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCCTGG

AGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGA

CCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGC

AGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCA

GCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGA

CGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGCACCC

TGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCA

GGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACC

TGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGT

ACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGC

GTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTG

AGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGAC

AGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCA

GACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCAC

CGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGG

GCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAGC

ACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTGCAG

AGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCTACAG

GGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGT

GCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACT

TCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACA

AGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCA

CCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACC

CCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACAC

CAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGG

TGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTG

TACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGAT

CGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCG

GCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCGGC

AGCGCCAGCAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAG

CCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCA

TCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGA

GCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACA

GCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCACCC

AGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACAAGAAC

ACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCCCCC

ATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCGACCCC

AGCAAGCCCAGCAAGAGGAGCCCCATCGAGGACCTGCTGTTCAACAAG

GTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCT

GGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACG

GCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAG

TACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTT

CGGCGCCGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATGGCCT

ACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACC

AGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGG

ACAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGTG

GTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAG

CAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAG

GCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCG

GCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGG

GCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAG

CGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGG

GCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTG

TTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACC

GCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGG

CGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTT

CTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAA

CTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCT

GCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCA

ACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAG

GTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGG

CAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTT

CATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTG

CATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGGCA

GCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGC

GTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCTGTTA

AAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTAGCGCCGTGAA

GAGGACCATCAAGGGCACCCACCACTGGCTGCTGCTGACCATCCTGAC

CAGCCTGCTGGTGCTGGTGCAGAGCACCCAGTGGAGCCTGTTCTTCTT

CCTGTACGAGAACGCCTTCCTGCCCTTCGCCATGGGCATCATCGCCAT

GAGCGCCTTCGCCATGATGTTCGTGAAGCACAAGCACGCCTTCCTGTG

CCTGTTCCTGCTGCCCAGCCTGGCCACCGTGGCCTACTTCAACATGGT

GTACATGCCCGCCAGCTGGGTGATGAGGATCATGACCTGGCTGGACAT

GGTGGACACCAGCCTGAGCGGCTTCAAGCTGAAGGACTGCGTGATGTA

CGCCAGCGCCGTGGTGCTGCTGATCCTGATGACCGCCAGGACCGTGT

ACGACGACGGCGCCAGGAGGGTGTGGACCCTGATGAACGTGCTGACC

CTGGTGTACAAGGTGTACTACGGCAACGCCCTGGACCAGGCCATCAGC

ATGTGGGCCCTGATCATCAGCGTGACCAGCAACTACAGCGGCGTGGTG

ACCACCGTGATGTTCCTGGCCAGGGGCATCGTGTTCATGTGCGTGGAG

TACTGCCCCATCTTCTTCATCACCGGCAACACCCTGCAGTGCATCATGC

TGGTGTACTGCTTCCTGGGCTACTTCTGCACCTGCTACTTCGGCCTGTT

CTGCCTGCTGAACAGGTACTTCAGGCTGACCCTGGGCGTGTACGACTA

CCTGGTGAGCACCCAGGAGTTCAGGTACATGAACAGCCAGGGCCTGCT

GCCCCCCAAGAACAGCATCGACGCCTTCAAGCTGAACATCAAGCTGCT

GGGCGTGGGCGGCAAGCCCTGCATCAAGGTGGCCACCGTGCAGGGAA

GCGGAGAGGGCAGGGGAAGTCTTCTAACATGCGGGGACGTGGAGGAA

AATCCCGGCCCCAGCAAGATGAGCGACGTGAAGTGCACCAGCGTGGT

GCTGCTGAGCGTGCTGCAGCAGCTGAGGGTGGAGAGCAGCAGCAAGC

TGTGGGCCCAGTGCGTGCAGCTGCACAACGACATCCTGCTGGCCAAG

GACACCACCGAGGCCTTCGAGAAGATGGTGAGCCTGCTGAGCGTGCT

GCTGAGCATGCAGGGCGCCGTGGACATCAACAAGCTGTGCGAGGAGA

TGCTGGACAACAGGGCCACCCTGCAGGGAAGCGGACAGTGTACTAATT

ATGCTCTCTTGAAATTGGCTGGAGATGTTGAGAGCAACCCAGGTCCCG

CCATCGCCAGCGAGTTCAGCAGCCTGCCCAGCTACGCCGCCTTCGCCA

CCGCCCAGGAGGCCTACGAGCAGGCCGTGGCCAACGGCGACAGCGA

GGTGGTGCTGAAGAAGCTGAAGAAGAGCCTGAACGTGGCCAAGAGCG

AGTTCGACAGGGACGCCGCCATGCAGAGGAAGCTGGAGAAGATGGCC

GACCAGGCCATGACCCAGATGTACAAGCAGGCCAGGAGCGAGGACAA

GAGGGCCAAGGTGACCAGCGCCATGCAGACCATGCTGTTCACCATGCT

GAGGAAGCTGGACAACGACGCCCTGAACAACATCATCAACAACGCCAG

GGACGGCTGCGTGCCCCTGAACATCATCCCCCTGACCACCGCCGCCA

AGCTGATGGTGGTGATCCCCGACTACAACACCTACAAGAACACCTGCG

ACGGCACCACCTTCACCTACGCCAGCGCCCTGTGGGAGATCCAGCAG

GTGGTGGACGCCGACAGCAAGATCGTGCAGCTGAGCGAGATCAGCAT

GGACAACAGCCCCAACCTGGCCTGGCCCCTGATCGTGACCGCCCTGA

GGGCCAACAGCGCCGTGAAGCTGCAGTGATGA

28
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGTG
311

Pan-CoV
AACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAGCTTC

mRNA Spike-
ACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCT

NSP9-NSP10-
GCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTG

NSP13
GTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGA

CAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAG

CAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGAT

CAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTA

CTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTA

CAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCT

GATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGT

TCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACAC

CCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCCTGG

AGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGA

CCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGC

AGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCA

GCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGA

CGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGCACCC

TGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCA

GGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACC

TGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGT

ACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGC

GTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTG

AGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGAC

AGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCA

GACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCAC

CGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGG

GCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAGC

ACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTGCAG

AGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCTACAG

GGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGT

GCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACT

TCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACA

AGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCA

CCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACC

CCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACAC

CAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGG

TGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTG

TACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGAT

CGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCG

GCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCAGG

AGGGCCAGGAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAG

CCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCA

TCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGA

GCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACA

GCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCACCC

AGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACAAGAAC

ACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCCCCC

ATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCGACCCC

AGCAAGCCCAGCAAGAGGAGCTTCATCGAGGACCTGCTGTTCAACAAG

GTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCT

GGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACG

GCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAG

TACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTT

CGGCGCCGGCGCCGCCCTGCAGATCCCCTTCGCCATGCAGATGGCCT

ACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACC

AGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGG

ACAGCCTGAGCAGCACCGCCAGCGCCCTGGGCAAGCTGCAGGACGTG

GTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAG

CAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAG

GCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCG

GCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGG

GCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAG

CGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGG

GCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTG

TTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACC

GCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGG

CGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTT

CTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAA

CTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCT

GCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCA

ACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAG

GTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGG

CAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTT

CATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTG

CATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGGCA

GCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGC

GTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCTGTTA

AAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTAACAACGAGCTG

AGCCCCGTGGCCCTGAGGCAGATGAGCTGCGCCGCCGGCACCACCCA

GACCGCCTGCACCGACGACAACGCCCTGGCCTACTACAACACCACCAA

GGGCGGCAGGTTCGTGCTGGCCCTGCTGAGCGACCTGCAGGACCTGA

AGTGGGCCAGGTTCCCCAAGAGCGACGGCACCGGCACCATCTACACC

GAGCTGGAGCCCCCCTGCAGGTTCGTGACCGACACCCCCAAGGGCCC

CAAGGTGAAGTACCTGTACTTCATCAAGGGCCTGAACAACCTGAACAG

GGGCATGGTGCTGGGCAGCCTGGCCGCCACCGTGAGGCTGCAGGGAA

GCGGAGAGGGCAGGGGAAGTCTTCTAACATGCGGGGACGTGGAGGAA

AATCCCGGCCCCGCCGGCAACGCCACCGAGGTGCCCGCCAACAGCAC

CGTGCTGAGCTTCTGCGCCTTCGCCGTGGACGCCGCCAAGGCCTACAA

GGACTACCTGGCCAGCGGCGGCCAGCCCATCACCAACTGCGTGAAGA

TGCTGTGCACCCACACCGGCACCGGCCAGGCCATCACCGTGACCCCC

GAGGCCAACATGGACCAGGAGAGCTTCGGCGGCGCCAGCTGCTGCCT

GTACTGCAGGTGCCACATCGACCACCCCAACCCCAAGGGCTTCTGCGA

CCTGAAGGGCAAGTACGTGCAGATCCCCACCACCTGCGCCAACGACCC

CGTGGGCTTCACCCTGAAGAACACCGTGTGCACCGTGTGCGGCATGTG

GAAGGGCTACGGCTGCAGCTGCGACCAGCTGAGGGAGCCCATGCTGC

AGGGAAGCGGACAGTGTACTAATTATGCTCTCTTGAAATTGGCTGGAGA

TGTTGAGAGCAACCCAGGTCCCGCCGTGGGCGCCTGCGTGCTGTGCA

ACAGCCAGACCAGCCTGAGGTGCGGCGCCTGCATCAGGAGGCCCTTC

CTGTGCTGCAAGTGCTGCTACGACCACGTGATCAGCACCAGCCACAAG

CTGGTGCTGAGCGTGAACCCCTACGTGTGCAACGCCCCCGGCTGCGA

CGTGACCGACGTGACCCAGCTGTACCTGGGCGGCATGAGCTACTACTG

CAAGAGCCACAAGCCCCCCATCAGCTTCCCCCTGTGCGCCAACGGCCA

GGTGTTCGGCCTGTACAAGAACACCTGCGTGGGCAGCGACAACGTGAC

CGACTTCAACGCCATCGCCACCTGCGACTGGACCAACGCCGGCGACTA

CATCCTGGCCAACACCTGCACCGAGAGGCTGAAGCTGTTCGCCGCCGA

GACCCTGAAGGCCACCGAGGAGACCTTCAAGCTGAGCTACGGCATCG

CCACCGTGAGGGAGGTGCTGAGCGACAGGGAGCTGCACCTGAGCTGG

GAGGTGGGCAAGCCCAGGCCCCCCCTGAACAGGAACTACGTGTTCAC

CGGCTACAGGGTGACCAAGAACAGCAAGGTGCAGATCGGCGAGTACA

CCTTCGAGAAGGGCGACTACGGCGACGCCGTGGTGTACAGGGGCACC

ACCACCTACAAGCTGAACGTGGGCGACTACTTCGTGCTGACCAGCCAC

ACCGTGATGCCCCTGAGCGCCCCCACCCTGGTGCCCCAGGAGCACTA

CGTGAGGATCACCGGCCTGTACCCCACCCTGAACATCAGCGACGAGTT

CAGCAGCAACGTGGCCAACTACCAGAAGGTGGGCATGCAGAAGTACAG

CACCCTGCAGGGCCCCCCCGGCACCGGCAAGAGCCACTTCGCCATCG

GCCTGGCCCTGTACTACCCCAGCGCCAGGATCGTGTACACCGCCTGCA

GCCACGCCGCCGTGGACGCCCTGTGCGAGAAGGCCCTGAAGTACCTG

CCCATCGACAAGTGCAGCAGGATCATCCCCGCCAGGGCCAGGGTGGA

GTGCTTCGACAAGTTCAAGGTGAACAGCACCCTGGAGCAGTACGTGTT

CTGCACCGTGAACGCCCTGCCCGAGACCACCGCCGACATCGTGGTGTT

CGACGAGATCAGCATGGCCACCAACTACGACCTGAGCGTGGTGAACGC

CAGGCTGAGGGCCAAGCACTACGTGTACATCGGCGACCCCGCCCAGC

TGCCCGCCCCCAGGACCCTGCTGACCAAGGGCACCCTGGAGCCCGAG

TACTTCAACAGCGTGTGCAGGCTGATGAAGACCATCGGCCCCGACATG

TTCCTGGGCACCTGCAGGAGGTGCCCCGCCGAGATCGTGGACACCGT

GAGCGCCCTGGTGTACGACAACAAGCTGAAGGCCCACAAGGACAAGA

GCGCCCAGTGCTTCAAGATGTTCTACAAGGGCGTGATCACCCACGACG

TGAGCAGCGCCATCAACAGGCCCCAGATCGGCGTGGTGAGGGAGTTC

CTGACCAGGAACCCCGCCTGGAGGAAGGCCGTGTTCATCAGCCCCTAC

AACAGCCAGAACGCCGTGGCCAGCAAGATCCTGGGCCTGCCCACCCA

GACCGTGGACAGCAGCCAGGGCAGCGAGTACGACTACGTGATCTTCAC

CCAGACCACCGAGACCGCCCACAGCTGCAACGTGAACAGGTTCAACGT

GGCCATCACCAGGGCCAAGGTGGGCATCCTGTGCATCATGAGCGACA

GGGACCTGTACGACAAGCTGCAGTTCACCAGCCTGGAGATCCCCAGGA

GGAACGTGGCCACCCTGCAGTGATGA

27
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGTG
312

Pan-CoV
AACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAGCTTC

mRNA
ACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCT

HexaPro-
GCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTG

NSP9-NSP10-
GTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGA

NSP13
CAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAG

CAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGAT

CAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTA

CTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTA

CAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCT

GATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGT

TCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACAC

CCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCCTGG

AGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGA

CCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGC

AGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCA

GCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGA

CGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGCACCC

TGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCA

GGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACC

TGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGT

ACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGC

GTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTG

AGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGAC

AGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCA

GACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCAC

CGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGG

GCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAGC

ACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTGCAG

AGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCTACAG

GGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGT

GCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACT

TCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACA

AGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCA

CCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACC

CCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACAC

CAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGG

TGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTG

TACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGAT

CGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCG

GCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCGGC

AGCGCCAGCAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAG

CCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCA

TCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGA

GCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACA

GCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCACCC

AGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACAAGAAC

ACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCCCCC

ATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCGACCCC

AGCAAGCCCAGCAAGAGGAGCCCCATCGAGGACCTGCTGTTCAACAAG

GTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCT

GGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACG

GCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAG

TACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTT

CGGCGCCGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATGGCCT

ACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACC

AGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGG

ACAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGTG

GTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAG

CAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAG

GCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCG

GCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGG

GCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAG

CGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGG

GCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTG

TTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACC

GCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGG

CGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTT

CTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAA

CTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCT

GCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCA

ACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAG

GTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGG

CAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTT

CATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTG

CATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGGCA

GCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGC

GTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCTGTTA

AAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTAACAACGAGCTG

AGCCCCGTGGCCCTGAGGCAGATGAGCTGCGCCGCCGGCACCACCCA

GACCGCCTGCACCGACGACAACGCCCTGGCCTACTACAACACCACCAA

GGGCGGCAGGTTCGTGCTGGCCCTGCTGAGCGACCTGCAGGACCTGA

AGTGGGCCAGGTTCCCCAAGAGCGACGGCACCGGCACCATCTACACC

GAGCTGGAGCCCCCCTGCAGGTTCGTGACCGACACCCCCAAGGGCCC

CAAGGTGAAGTACCTGTACTTCATCAAGGGCCTGAACAACCTGAACAG

GGGCATGGTGCTGGGCAGCCTGGCCGCCACCGTGAGGCTGCAGGGAA

GCGGAGAGGGCAGGGGAAGTCTTCTAACATGCGGGGACGTGGAGGAA

AATCCCGGCCCCGCCGGCAACGCCACCGAGGTGCCCGCCAACAGCAC

CGTGCTGAGCTTCTGCGCCTTCGCCGTGGACGCCGCCAAGGCCTACAA

GGACTACCTGGCCAGCGGCGGCCAGCCCATCACCAACTGCGTGAAGA

TGCTGTGCACCCACACCGGCACCGGCCAGGCCATCACCGTGACCCCC

GAGGCCAACATGGACCAGGAGAGCTTCGGCGGCGCCAGCTGCTGCCT

GTACTGCAGGTGCCACATCGACCACCCCAACCCCAAGGGCTTCTGCGA

CCTGAAGGGCAAGTACGTGCAGATCCCCACCACCTGCGCCAACGACCC

CGTGGGCTTCACCCTGAAGAACACCGTGTGCACCGTGTGCGGCATGTG

GAAGGGCTACGGCTGCAGCTGCGACCAGCTGAGGGAGCCCATGCTGC

AGGGAAGCGGACAGTGTACTAATTATGCTCTCTTGAAATTGGCTGGAGA

TGTTGAGAGCAACCCAGGTCCCGCCGTGGGCGCCTGCGTGCTGTGCA

ACAGCCAGACCAGCCTGAGGTGCGGCGCCTGCATCAGGAGGCCCTTC

CTGTGCTGCAAGTGCTGCTACGACCACGTGATCAGCACCAGCCACAAG

CTGGTGCTGAGCGTGAACCCCTACGTGTGCAACGCCCCCGGCTGCGA

CGTGACCGACGTGACCCAGCTGTACCTGGGCGGCATGAGCTACTACTG

CAAGAGCCACAAGCCCCCCATCAGCTTCCCCCTGTGCGCCAACGGCCA

GGTGTTCGGCCTGTACAAGAACACCTGCGTGGGCAGCGACAACGTGAC

CGACTTCAACGCCATCGCCACCTGCGACTGGACCAACGCCGGCGACTA

CATCCTGGCCAACACCTGCACCGAGAGGCTGAAGCTGTTCGCCGCCGA

GACCCTGAAGGCCACCGAGGAGACCTTCAAGCTGAGCTACGGCATCG

CCACCGTGAGGGAGGTGCTGAGCGACAGGGAGCTGCACCTGAGCTGG

GAGGTGGGCAAGCCCAGGCCCCCCCTGAACAGGAACTACGTGTTCAC

CGGCTACAGGGTGACCAAGAACAGCAAGGTGCAGATCGGCGAGTACA

CCTTCGAGAAGGGCGACTACGGCGACGCCGTGGTGTACAGGGGCACC

ACCACCTACAAGCTGAACGTGGGCGACTACTTCGTGCTGACCAGCCAC

ACCGTGATGCCCCTGAGCGCCCCCACCCTGGTGCCCCAGGAGCACTA

CGTGAGGATCACCGGCCTGTACCCCACCCTGAACATCAGCGACGAGTT

CAGCAGCAACGTGGCCAACTACCAGAAGGTGGGCATGCAGAAGTACAG

CACCCTGCAGGGCCCCCCCGGCACCGGCAAGAGCCACTTCGCCATCG

GCCTGGCCCTGTACTACCCCAGCGCCAGGATCGTGTACACCGCCTGCA

GCCACGCCGCCGTGGACGCCCTGTGCGAGAAGGCCCTGAAGTACCTG

CCCATCGACAAGTGCAGCAGGATCATCCCCGCCAGGGCCAGGGTGGA

GTGCTTCGACAAGTTCAAGGTGAACAGCACCCTGGAGCAGTACGTGTT

CTGCACCGTGAACGCCCTGCCCGAGACCACCGCCGACATCGTGGTGTT

CGACGAGATCAGCATGGCCACCAACTACGACCTGAGCGTGGTGAACGC

CAGGCTGAGGGCCAAGCACTACGTGTACATCGGCGACCCCGCCCAGC

TGCCCGCCCCCAGGACCCTGCTGACCAAGGGCACCCTGGAGCCCGAG

TACTTCAACAGCGTGTGCAGGCTGATGAAGACCATCGGCCCCGACATG

TTCCTGGGCACCTGCAGGAGGTGCCCCGCCGAGATCGTGGACACCGT

GAGCGCCCTGGTGTACGACAACAAGCTGAAGGCCCACAAGGACAAGA

GCGCCCAGTGCTTCAAGATGTTCTACAAGGGCGTGATCACCCACGACG

TGAGCAGCGCCATCAACAGGCCCCAGATCGGCGTGGTGAGGGAGTTC

CTGACCAGGAACCCCGCCTGGAGGAAGGCCGTGTTCATCAGCCCCTAC

AACAGCCAGAACGCCGTGGCCAGCAAGATCCTGGGCCTGCCCACCCA

GACCGTGGACAGCAGCCAGGGCAGCGAGTACGACTACGTGATCTTCAC

CCAGACCACCGAGACCGCCCACAGCTGCAACGTGAACAGGTTCAACGT

GGCCATCACCAGGGCCAAGGTGGGCATCCTGTGCATCATGAGCGACA

GGGACCTGTACGACAAGCTGCAGTTCACCAGCCTGGAGATCCCCAGGA

GGAACGTGGCCACCCTGCAGTGATGA

28
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGTG
313

Pan-CoV
AACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAGCTTC

mRNA Spike-
ACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCT

NSP14-
GCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTG

NSP15
GTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGA

CAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAG

CAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGAT

CAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTA

CTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTA

CAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCT

GATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGT

TCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACAC

CCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCCTGG

AGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGA

CCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGC

AGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCA

GCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGA

CGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGCACCC

TGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCA

GGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACC

TGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGT

ACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGC

GTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTG

AGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGAC

AGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCA

GACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCAC

CGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGG

GCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAGC

ACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTGCAG

AGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCTACAG

GGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGT

GCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACT

TCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACA

AGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCA

CCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACC

CCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACAC

CAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGG

TGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTG

TACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGAT

CGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCG

GCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCAGG

AGGGCCAGGAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAG

CCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCA

TCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGA

GCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACA

GCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCACCC

AGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACAAGAAC

ACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCCCCC

ATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCGACCCC

AGCAAGCCCAGCAAGAGGAGCTTCATCGAGGACCTGCTGTTCAACAAG

GTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCT

GGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACG

GCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAG

TACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTT

CGGCGCCGGCGCCGCCCTGCAGATCCCCTTCGCCATGCAGATGGCCT

ACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACC

AGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGG

ACAGCCTGAGCAGCACCGCCAGCGCCCTGGGCAAGCTGCAGGACGTG

GTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAG

CAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAG

GCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCG

GCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGG

GCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAG

CGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGG

GCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTG

TTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACC

GCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGG

CGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTT

CTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAA

CTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCT

GCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCA

ACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAG

GTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGG

CAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTT

CATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTG

CATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGGCA

GCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGC

GTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCTGTTA

AAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTGCCGAGAACGT

GACCGGCCTGTTCAAGGACTGCAGCAAGGTGATCACCGGCCTGCACC

CCACCCAGGCCCCCACCCACCTGAGCGTGGACACCAAGTTCAAGACC

GAGGGCCTGTGCGTGGACATCCCCGGCATCCCCAAGGACATGACCTA

CAGGAGGCTGATCAGCATGATGGGCTTCAAGATGAACTACCAGGTGAA

CGGCTACCCCAACATGTTCATCACCAGGGAGGAGGCCATCAGGCACGT

GAGGGCCTGGATCGGCTTCGACGTGGAGGGCTGCCACGCCACCAGGG

AGGCCGTGGGCACCAACCTGCCCCTGCAGCTGGGCTTCAGCACCGGC

GTGAACCTGGTGGCCGTGCCCACCGGCTACGTGGACACCCCCAACAA

CACCGACTTCAGCAGGGTGAGCGCCAAGCCCCCCCCCGGCGACCAGT

TCAAGCACCTGATCCCCCTGATGTACAAGGGCCTGCCCTGGAACGTGG

TGAGGATCAAGATCGTGCAGATGCTGAGCGACACCCTGAAGAACCTGA

GCGACAGGGTGGTGTTCGTGCTGTGGGCCCACGGCTTCGAGCTGACC

AGCATGAAGTACTTCGTGAAGATCGGCCCCGAGAGGACCTGCTGCCTG

TGCGACAGGAGGGCCACCTGCTTCAGCACCGCCAGCGACACCTACGC

CTGCTGGCACCACAGCATCGGCTTCGACTACGTGTACAACCCCTTCAT

GATCGACGTGCAGCAGTGGGGCTTCACCGGCAACCTGCAGAGCAACC

ACGACCTGTACTGCCAGGTGCACGGCAACGCCCACGTGGCCAGCTGC

GACGCCATCATGACCAGGTGCCTGGCCGTGCACGAGTGCTTCGTGAAG

AGGGTGGACTGGACCATCGAGTACCCCATCATCGGCGACGAGCTGAA

GATCAACGCCGCCTGCAGGAAGGTGCAGCACATGGTGGTGAAGGCCG

CCCTGCTGGCCGACAAGTTCCCCGTGCTGCACGACATCGGCAACCCCA

AGGCCATCAAGTGCGTGCCCCAGGCCGACGTGGAGTGGAAGTTCTAC

GACGCCCAGCCCTGCAGCGACAAGGCCTACAAGATCGAGGAGCTGTT

CTACAGCTACGCCACCCACAGCGACAAGTTCACCGACGGCGTGTGCCT

GTTCTGGAACTGCAACGTGGACAGGTACCCCGCCAACAGCATCGTGTG

CAGGTTCGACACCAGGGTGCTGAGCAACCTGAACCTGCCCGGCTGCG

ACGGCGGCAGCCTGTACGTGAACAAGCACGCCTTCCACACCCCCGCCT

TCGACAAGAGCGCCTTCGTGAACCTGAAGCAGCTGCCCTTCTTCTACTA

CAGCGACAGCCCCTGCGAGAGCCACGGCAAGCAGGTGGTGAGCGACA

TCGACTACGTGCCCCTGAAGAGCGCCACCTGCATCACCAGGTGCAACC

TGGGCGGCGCCGTGTGCAGGCACCACGCCAACGAGTACAGGCTGTAC

CTGGACGCCTACAACATGATGATCAGCGCCGGCTTCAGCCTGTGGGTG

TACAAGCAGTTCGACACCTACAACCTGTGGAACACCTTCACCAGGCTG

CAGGGAAGCGGAGAGGGCAGGGGAAGTCTTCTAACATGCGGGGACGT

GGAGGAAAATCCCGGCCCCAGCCTGGAGAACGTGGCCTTCAACGTGG

TGAACAAGGGCCACTTCGACGGCCAGCAGGGCGAGGTGCCCGTGAGC

ATCATCAACAACACCGTGTACACCAAGGTGGACGGCGTGGACGTGGAG

CTGTTCGAGAACAAGACCACCCTGCCCGTGAACGTGGCCTTCGAGCTG

TGGGCCAAGAGGAACATCAAGCCCGTGCCCGAGGTGAAGATCCTGAAC

AACCTGGGCGTGGACATCGCCGCCAACACCGTGATCTGGGACTACAAG

AGGGACGCCCCCGCCCACATCAGCACCATCGGCGTGTGCAGCATGAC

CGACATCGCCAAGAAGCCCACCGAGACCATCTGCGCCCCCCTGACCGT

GTTCTTCGACGGCAGGGTGGACGGCCAGGTGGACCTGTTCAGGAACG

CCAGGAACGGCGTGCTGATCACCGAGGGCAGCGTGAAGGGCCTGCAG

CCCAGCGTGGGCCCCAAGCAGGCCAGCCTGAACGGCGTGACCCTGAT

CGGCGAGGCCGTGAAGACCCAGTTCAACTACTACAAGAAGGTGGACG

GCGTGGTGCAGCAGCTGCCCGAGACCTACTTCACCCAGAGCAGGAAC

CTGCAGGAGTTCAAGCCCAGGAGCCAGATGGAGATCGACTTCCTGGAG

CTGGCCATGGACGAGTTCATCGAGAGGTACAAGCTGGAGGGCTACGC

CTTCGAGCACATCGTGTACGGCGACTTCAGCCACAGCCAGCTGGGCG

GCCTGCACCTGCTGATCGGCCTGGCCAAGAGGTTCAAGGAGAGCCCC

TTCGAGCTGGAGGACTTCATCCCCATGGACAGCACCGTGAAGAACTAC

TTCATCACCGACGCCCAGACCGGCAGCAGCAAGTGCGTGTGCAGCGT

GATCGACCTGCTGCTGGACGACTTCGTGGAGATCATCAAGAGCCAGGA

CCTGAGCGTGGTGAGCAAGGTGGTGAAGGTGACCATCGACTACACCGA

GATCAGCTTCATGCTGTGGTGCAAGGACGGCCACGTGGAGACCTTCTA

CCCCAAGCTGCAGTGATGA

29
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGTG
314

Pan-CoV
AACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAGCTTC

mRNA
ACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCT

HexaPro-
GCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTG

NSP14-
GTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGA

NSP15
CAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAG

CAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGAT

CAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTA

CTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTA

CAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCT

GATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGT

TCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACAC

CCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCCTGG

AGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGA

CCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGC

AGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCA

GCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGA

CGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGCACCC

TGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCA

GGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACC

TGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGT

ACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGC

GTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTG

AGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGAC

AGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCA

GACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCAC

CGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGG

GCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAGC

ACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTGCAG

AGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCTACAG

GGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGT

GCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACT

TCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACA

AGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCA

CCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACC

CCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACAC

CAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGG

TGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTG

TACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGAT

CGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCG

GCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCGGC

AGCGCCAGCAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAG

CCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCA

TCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGA

GCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACA

GCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCACCC

AGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACAAGAAC

ACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCCCCC

ATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCGACCCC

AGCAAGCCCAGCAAGAGGAGCCCCATCGAGGACCTGCTGTTCAACAAG

GTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCT

GGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACG

GCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAG

TACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTT

CGGCGCCGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATGGCCT

ACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACC

AGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGG

ACAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGTG

GTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAG

CAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAG

GCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCG

GCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGG

GCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAG

CGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGG

GCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTG

TTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACC

GCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGG

CGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTT

CTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAA

CTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCT

GCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCA

ACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAG

GTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGG

CAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTT

CATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTG

CATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGGCA

GCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGC

GTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCTGTTA

AAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTGCCGAGAACGT

GACCGGCCTGTTCAAGGACTGCAGCAAGGTGATCACCGGCCTGCACC

CCACCCAGGCCCCCACCCACCTGAGCGTGGACACCAAGTTCAAGACC

GAGGGCCTGTGCGTGGACATCCCCGGCATCCCCAAGGACATGACCTA

CAGGAGGCTGATCAGCATGATGGGCTTCAAGATGAACTACCAGGTGAA

CGGCTACCCCAACATGTTCATCACCAGGGAGGAGGCCATCAGGCACGT

GAGGGCCTGGATCGGCTTCGACGTGGAGGGCTGCCACGCCACCAGGG

AGGCCGTGGGCACCAACCTGCCCCTGCAGCTGGGCTTCAGCACCGGC

GTGAACCTGGTGGCCGTGCCCACCGGCTACGTGGACACCCCCAACAA

CACCGACTTCAGCAGGGTGAGCGCCAAGCCCCCCCCCGGCGACCAGT

TCAAGCACCTGATCCCCCTGATGTACAAGGGCCTGCCCTGGAACGTGG

TGAGGATCAAGATCGTGCAGATGCTGAGCGACACCCTGAAGAACCTGA

GCGACAGGGTGGTGTTCGTGCTGTGGGCCCACGGCTTCGAGCTGACC

AGCATGAAGTACTTCGTGAAGATCGGCCCCGAGAGGACCTGCTGCCTG

TGCGACAGGAGGGCCACCTGCTTCAGCACCGCCAGCGACACCTACGC

CTGCTGGCACCACAGCATCGGCTTCGACTACGTGTACAACCCCTTCAT

GATCGACGTGCAGCAGTGGGGCTTCACCGGCAACCTGCAGAGCAACC

ACGACCTGTACTGCCAGGTGCACGGCAACGCCCACGTGGCCAGCTGC

GACGCCATCATGACCAGGTGCCTGGCCGTGCACGAGTGCTTCGTGAAG

AGGGTGGACTGGACCATCGAGTACCCCATCATCGGCGACGAGCTGAA

GATCAACGCCGCCTGCAGGAAGGTGCAGCACATGGTGGTGAAGGCCG

CCCTGCTGGCCGACAAGTTCCCCGTGCTGCACGACATCGGCAACCCCA

AGGCCATCAAGTGCGTGCCCCAGGCCGACGTGGAGTGGAAGTTCTAC

GACGCCCAGCCCTGCAGCGACAAGGCCTACAAGATCGAGGAGCTGTT

CTACAGCTACGCCACCCACAGCGACAAGTTCACCGACGGCGTGTGCCT

GTTCTGGAACTGCAACGTGGACAGGTACCCCGCCAACAGCATCGTGTG

CAGGTTCGACACCAGGGTGCTGAGCAACCTGAACCTGCCCGGCTGCG

ACGGCGGCAGCCTGTACGTGAACAAGCACGCCTTCCACACCCCCGCCT

TCGACAAGAGCGCCTTCGTGAACCTGAAGCAGCTGCCCTTCTTCTACTA

CAGCGACAGCCCCTGCGAGAGCCACGGCAAGCAGGTGGTGAGCGACA

TCGACTACGTGCCCCTGAAGAGCGCCACCTGCATCACCAGGTGCAACC

TGGGCGGCGCCGTGTGCAGGCACCACGCCAACGAGTACAGGCTGTAC

CTGGACGCCTACAACATGATGATCAGCGCCGGCTTCAGCCTGTGGGTG

TACAAGCAGTTCGACACCTACAACCTGTGGAACACCTTCACCAGGCTG

CAGGGAAGCGGAGAGGGCAGGGGAAGTCTTCTAACATGCGGGGACGT

GGAGGAAAATCCCGGCCCCAGCCTGGAGAACGTGGCCTTCAACGTGG

TGAACAAGGGCCACTTCGACGGCCAGCAGGGCGAGGTGCCCGTGAGC

ATCATCAACAACACCGTGTACACCAAGGTGGACGGCGTGGACGTGGAG

CTGTTCGAGAACAAGACCACCCTGCCCGTGAACGTGGCCTTCGAGCTG

TGGGCCAAGAGGAACATCAAGCCCGTGCCCGAGGTGAAGATCCTGAAC

AACCTGGGCGTGGACATCGCCGCCAACACCGTGATCTGGGACTACAAG

AGGGACGCCCCCGCCCACATCAGCACCATCGGCGTGTGCAGCATGAC

CGACATCGCCAAGAAGCCCACCGAGACCATCTGCGCCCCCCTGACCGT

GTTCTTCGACGGCAGGGTGGACGGCCAGGTGGACCTGTTCAGGAACG

CCAGGAACGGCGTGCTGATCACCGAGGGCAGCGTGAAGGGCCTGCAG

CCCAGCGTGGGCCCCAAGCAGGCCAGCCTGAACGGCGTGACCCTGAT

CGGCGAGGCCGTGAAGACCCAGTTCAACTACTACAAGAAGGTGGACG

GCGTGGTGCAGCAGCTGCCCGAGACCTACTTCACCCAGAGCAGGAAC

CTGCAGGAGTTCAAGCCCAGGAGCCAGATGGAGATCGACTTCCTGGAG

CTGGCCATGGACGAGTTCATCGAGAGGTACAAGCTGGAGGGCTACGC

CTTCGAGCACATCGTGTACGGCGACTTCAGCCACAGCCAGCTGGGCG

GCCTGCACCTGCTGATCGGCCTGGCCAAGAGGTTCAAGGAGAGCCCC

TTCGAGCTGGAGGACTTCATCCCCATGGACAGCACCGTGAAGAACTAC

TTCATCACCGACGCCCAGACCGGCAGCAGCAAGTGCGTGTGCAGCGT

GATCGACCTGCTGCTGGACGACTTCGTGGAGATCATCAAGAGCCAGGA

CCTGAGCGTGGTGAGCAAGGTGGTGAAGGTGACCATCGACTACACCGA

GATCAGCTTCATGCTGTGGTGCAAGGACGGCCACGTGGAGACCTTCTA

CCCCAAGCTGCAGTGATGA

30
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGTG
315

Pan-CoV
AACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAGCTTC

mRNA Spike-
ACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCT

NSP16
GCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTG

GTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGA

CAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAG

CAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGAT

CAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTA

CTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTA

CAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCT

GATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGT

TCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACAC

CCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCCTGG

AGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGA

CCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGC

AGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCA

GCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGA

CGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGCACCC

TGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCA

GGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACC

TGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGT

ACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGC

GTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTG

AGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGAC

AGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCA

GACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCAC

CGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGG

GCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAGC

ACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTGCAG

AGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCTACAG

GGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGT

GCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACT

TCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACA

AGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCA

CCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACC

CCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACAC

CAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGG

TGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTG

TACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGAT

CGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCG

GCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCAGG

AGGGCCAGGAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAG

CCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCA

TCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGA

GCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACA

GCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCACCC

AGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACAAGAAC

ACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCCCCC

ATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCGACCCC

AGCAAGCCCAGCAAGAGGAGCTTCATCGAGGACCTGCTGTTCAACAAG

GTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCT

GGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACG

GCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAG

TACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTT

CGGCGCCGGCGCCGCCCTGCAGATCCCCTTCGCCATGCAGATGGCCT

ACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACC

AGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGG

ACAGCCTGAGCAGCACCGCCAGCGCCCTGGGCAAGCTGCAGGACGTG

GTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAG

CAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAG

GCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCG

GCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGG

GCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAG

CGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGG

GCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTG

TTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACC

GCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGG

CGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTT

CTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAA

CTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCT

GCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCA

ACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAG

GTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGG

CAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTT

CATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTG

CATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGGCA

GCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGC

GTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCTGTTA

AAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTAGCAGCCAGGC

CTGGCAGCCCGGCGTGGCCATGCCCAACCTGTACAAGATGCAGAGGA

TGCTGCTGGAGAAGTGCGACCTGCAGAACTACGGCGACAGCGCCACC

CTGCCCAAGGGCATCATGATGAACGTGGCCAAGTACACCCAGCTGTGC

CAGTACCTGAACACCCTGACCCTGGCCGTGCCCTACAACATGAGGGTG

ATCCACTTCGGCGCCGGCAGCGACAAGGGCGTGGCCCCCGGCACCGC

CGTGCTGAGGCAGTGGCTGCCCACCGGCACCCTGCTGGTGGACAGCG

ACCTGAACGACTTCGTGAGCGACGCCGACAGCACCCTGATCGGCGACT

GCGCCACCGTGCACACCGCCAACAAGTGGGACCTGATCATCAGCGACA

TGTACGACCCCAAGACCAAGAACGTGACCAAGGAGAACGACAGCAAGG

AGGGCTTCTTCACCTACATCTGCGGCTTCATCCAGCAGAAGCTGGCCC

TGGGCGGCAGCGTGGCCATCAAGATCACCGAGCACAGCTGGAACGCC

GACCTGTACAAGCTGATGGGCCACTTCGCCTGGTGGACCGCCTTCGTG

ACCAACGTGAACGCCAGCAGCAGCGAGGCCTTCCTGATCGGCTGCAAC

TACCTGGGCAAGCCCAGGGAGCAGATCGACGGCTACGTGATGCACGC

CAACTACATCTTCTGGAGGAACACCAACCCCATCCAGCTGAGCAGCTA

CAGCCTGTTCGACATGAGCAAGTTCCCCCTGAAGCTGAGGGGCACCGC

CGTGATGAGCCTGAAGGAGGGCCAGATCAACGACATGATCCTGAGCCT

GCTGAGCAAGGGCAGGCTGATCATCAGGGAGAACAACAGGGTGGTGA

TCAGCAGCGACGTGCTGGTGAACAACTGATGA

31
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGTG
316

Pan-CoV
AACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAGCTTC

mRNA
ACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCT

HexaPro-
GCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTG

NSP16
GTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGA

CAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAG

CAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGAT

CAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTA

CTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTA

CAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCT

GATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGT

TCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACAC

CCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCCTGG

AGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGA

CCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGC

AGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCA

GCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGA

CGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGCACCC

TGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCA

GGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACC

TGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGT

ACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGC

GTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTG

AGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGAC

AGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCA

GACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCAC

CGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGG

GCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAGC

ACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTGCAG

AGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCTACAG

GGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGT

GCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACT

TCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACA

AGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCA

CCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACC

CCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACAC

CAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGG

TGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTG

TACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGAT

CGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCG

GCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCGGC

AGCGCCAGCAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAG

CCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCA

TCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGA

GCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACA

GCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCACCC

AGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACAAGAAC

ACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCCCCC

ATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCGACCCC

AGCAAGCCCAGCAAGAGGAGCCCCATCGAGGACCTGCTGTTCAACAAG

GTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCT

GGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACG

GCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAG

TACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTT

CGGCGCCGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATGGCCT

ACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACC

AGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGG

ACAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGTG

GTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAG

CAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAG

GCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCG

GCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGG

GCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAG

CGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGG

GCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTG

TTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACC

GCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGG

CGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTT

CTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAA

CTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCT

GCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCA

ACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAG

GTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGG

CAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTT

CATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTG

CATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGGCA

GCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGC

GTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCTGTTA

AAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTAGCAGCCAGGC

CTGGCAGCCCGGCGTGGCCATGCCCAACCTGTACAAGATGCAGAGGA

TGCTGCTGGAGAAGTGCGACCTGCAGAACTACGGCGACAGCGCCACC

CTGCCCAAGGGCATCATGATGAACGTGGCCAAGTACACCCAGCTGTGC

CAGTACCTGAACACCCTGACCCTGGCCGTGCCCTACAACATGAGGGTG

ATCCACTTCGGCGCCGGCAGCGACAAGGGCGTGGCCCCCGGCACCGC

CGTGCTGAGGCAGTGGCTGCCCACCGGCACCCTGCTGGTGGACAGCG

ACCTGAACGACTTCGTGAGCGACGCCGACAGCACCCTGATCGGCGACT

GCGCCACCGTGCACACCGCCAACAAGTGGGACCTGATCATCAGCGACA

TGTACGACCCCAAGACCAAGAACGTGACCAAGGAGAACGACAGCAAGG

AGGGCTTCTTCACCTACATCTGCGGCTTCATCCAGCAGAAGCTGGCCC

TGGGCGGCAGCGTGGCCATCAAGATCACCGAGCACAGCTGGAACGCC

GACCTGTACAAGCTGATGGGCCACTTCGCCTGGTGGACCGCCTTCGTG

ACCAACGTGAACGCCAGCAGCAGCGAGGCCTTCCTGATCGGCTGCAAC

TACCTGGGCAAGCCCAGGGAGCAGATCGACGGCTACGTGATGCACGC

CAACTACATCTTCTGGAGGAACACCAACCCCATCCAGCTGAGCAGCTA

CAGCCTGTTCGACATGAGCAAGTTCCCCCTGAAGCTGAGGGGCACCGC

CGTGATGAGCCTGAAGGAGGGCCAGATCAACGACATGATCCTGAGCCT

GCTGAGCAAGGGCAGGCTGATCATCAGGGAGAACAACAGGGTGGTGA

TCAGCAGCGACGTGCTGGTGAACAACTGATGA

32
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGTG
317

Pan-CoV
AACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAGCTTC

mRNA Spike-
ACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCT

ORF3a
GCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTG

GTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGA

CAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAG

CAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGAT

CAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTA

CTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTA

CAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCT

GATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGT

TCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACAC

CCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCCTGG

AGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGA

CCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGC

AGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCA

GCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGA

CGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGCACCC

TGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCA

GGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACC

TGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGT

ACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGC

GTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTG

AGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGAC

AGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCA

GACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCAC

CGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGG

GCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAGC

ACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTGCAG

AGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCTACAG

GGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGT

GCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACT

TCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACA

AGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCA

CCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACC

CCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACAC

CAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGG

TGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTG

TACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGAT

CGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCG

GCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCAGG

AGGGCCAGGAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAG

CCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCA

TCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGA

GCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACA

GCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCACCC

AGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACAAGAAC

ACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCCCCC

ATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCGACCCC

AGCAAGCCCAGCAAGAGGAGCTTCATCGAGGACCTGCTGTTCAACAAG

GTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCT

GGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACG

GCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAG

TACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTT

CGGCGCCGGCGCCGCCCTGCAGATCCCCTTCGCCATGCAGATGGCCT

ACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACC

AGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGG

ACAGCCTGAGCAGCACCGCCAGCGCCCTGGGCAAGCTGCAGGACGTG

GTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAG

CAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAG

GCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCG

GCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGG

GCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAG

CGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGG

GCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTG

TTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACC

GCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGG

CGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTT

CTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAA

CTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCT

GCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCA

ACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAG

GTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGG

CAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTT

CATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTG

CATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGGCA

GCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGC

GTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCTGTTA

AAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTATGGACCTGTTC

ATGAGGATCTTCACCATCGGCACCGTGACCCTGAAGCAGGGCGAGATC

AAGGACGCCACCCCCAGCGACTTCGTGAGGGCCACCGCCACCATCCC

CATCCAGGCCAGCCTGCCCTTCGGCTGGCTGATCGTGGGCGTGGCCC

TGCTGGCCGTGTTCCAGAGCGCCAGCAAGATCATCACCCTGAAGAAGA

GGTGGCAGCTGGCCCTGAGCAAGGGCGTGCACTTCGTGTGCAACCTG

CTGCTGCTGTTCGTGACCGTGTACAGCCACCTGCTGCTGGTGGCCGCC

GGCCTGGAGGCCCCCTTCCTGTACCTGTACGCCCTGGTGTACTTCCTG

CAGAGCATCAACTTCGTGAGGATCATCATGAGGCTGTGGCTGTGCTGG

AAGTGCAGGAGCAAGAACCCCCTGCTGTACGACGCCAACTACTTCCTG

TGCTGGCACACCAACTGCTACGACTACTGCATCCCCTACAACAGCGTG

ACCAGCAGCATCGTGATCACCAGCGGCGACGGCACCACCAGCCCCAT

CAGCGAGCACGACTACCAGATCGGCGGCTACACCGAGAAGTGGGAGA

GCGGCGTGAAGGACTGCGTGGTGCTGCACAGCTACTTCACCAGCGACT

ACTACCAGCTGTACAGCACCCAGCTGAGCACCGACACCGGCGTGGAG

CACGTGACCTTCTTCATCTACAACAAGATCGTGGACGAGCCCGAGGAG

CACGTGCAGATCCACACCATCGACGGCAGCAGCGGCGTGGTGAACCC

CGTGATGGAGCCCATCTACGACGAGCCCACCACCACCACCAGCGTGC

CCCTGTGATGA

33
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGTG
318

Pan-CoV
AACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAGCTTC

mRNA
ACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCT

HexaPro-
GCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTG

ORF3a
GTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGA

CAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAG

CAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGAT

CAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTA

CTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTA

CAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCT

GATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGT

TCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACAC

CCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCCTGG

AGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGA

CCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGC

AGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCA

GCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGA

CGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGCACCC

TGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCA

GGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACC

TGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGT

ACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGC

GTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTG

AGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGAC

AGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCA

GACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCAC

CGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGG

GCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAGC

ACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTGCAG

AGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCTACAG

GGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGT

GCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACT

TCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACA

AGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCA

CCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACC

CCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACAC

CAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGG

TGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTG

TACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGAT

CGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCG

GCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCGGC

AGCGCCAGCAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAG

CCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCA

TCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGA

GCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACA

GCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCACCC

AGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACAAGAAC

ACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCCCCC

ATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCGACCCC

AGCAAGCCCAGCAAGAGGAGCCCCATCGAGGACCTGCTGTTCAACAAG

GTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCT

GGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACG

GCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAG

TACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTT

CGGCGCCGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATGGCCT

ACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACC

AGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGG

ACAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGTG

GTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAG

CAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAG

GCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCG

GCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGG

GCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAG

CGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGG

GCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTG

TTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACC

GCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGG

CGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTT

CTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAA

CTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCT

GCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCA

ACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAG

GTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGG

CAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTT

CATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTG

CATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGGCA

GCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGC

GTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCTGTTA

AAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTATGGACCTGTTC

ATGAGGATCTTCACCATCGGCACCGTGACCCTGAAGCAGGGCGAGATC

AAGGACGCCACCCCCAGCGACTTCGTGAGGGCCACCGCCACCATCCC

CATCCAGGCCAGCCTGCCCTTCGGCTGGCTGATCGTGGGCGTGGCCC

TGCTGGCCGTGTTCCAGAGCGCCAGCAAGATCATCACCCTGAAGAAGA

GGTGGCAGCTGGCCCTGAGCAAGGGCGTGCACTTCGTGTGCAACCTG

CTGCTGCTGTTCGTGACCGTGTACAGCCACCTGCTGCTGGTGGCCGCC

GGCCTGGAGGCCCCCTTCCTGTACCTGTACGCCCTGGTGTACTTCCTG

CAGAGCATCAACTTCGTGAGGATCATCATGAGGCTGTGGCTGTGCTGG

AAGTGCAGGAGCAAGAACCCCCTGCTGTACGACGCCAACTACTTCCTG

TGCTGGCACACCAACTGCTACGACTACTGCATCCCCTACAACAGCGTG

ACCAGCAGCATCGTGATCACCAGCGGCGACGGCACCACCAGCCCCAT

CAGCGAGCACGACTACCAGATCGGCGGCTACACCGAGAAGTGGGAGA

GCGGCGTGAAGGACTGCGTGGTGCTGCACAGCTACTTCACCAGCGACT

ACTACCAGCTGTACAGCACCCAGCTGAGCACCGACACCGGCGTGGAG

CACGTGACCTTCTTCATCTACAACAAGATCGTGGACGAGCCCGAGGAG

CACGTGCAGATCCACACCATCGACGGCAGCAGCGGCGTGGTGAACCC

CGTGATGGAGCCCATCTACGACGAGCCCACCACCACCACCAGCGTGC

CCCTGTGATGA

34
CTCGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCA
319

Pan-CoV
TTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAA

rAdv5-CAG-
TGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAAT

HexaPro
AATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGT

CAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAG

TGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATG

GCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACT

TGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGA

GCCCCACGTTCTGCTTCACTCTCCCCATCTCCCCCCCCTCCCCACCCC

CAATTTTGTATTTATTTATTTTTTAATTATTTTGTGCAGCGATGGGGGCG

GGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGGGGCGA

GGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCA

GAGCGGCGCGCTCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGC

GGCGGCCCTATAAAAAGCGAAGCGCGCGGCGGGCGGGAGTCGCTGC

GCGCTGCCTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCGCGCCGCC

CGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGG

GACGGCCCTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGG

CTTGTTTCTTTTCTGTGGCTGCGTGAAAGCCTTGAGGGGCTCCGGGAG

GGCCCTTTGTGCGGGGGGAGCGGCTCGGGGGGTGCGTGCGTGTGTG

TGTGCGTGGGGAGCGCCGCGTGCGGCTCCGCGCTGCCCGGCGGCTG

TGAGCGCTGCGGGCGCGGCGCGGGGCTTTGTGCGCTCCGCAGTGTGC

GCGAGGGGAGCGCGGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGG

GCTGCGAGGGGAACAAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGG

GTGAGCAGGGGGTGTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTG

CACCCCCCTCCCCGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGG

GCTCCGTACGGGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGG

TGGCGGCAGGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCC

GGGGAGGGCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGC

GGCTGTCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCG

TGCGAGAGGGCGCAGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCG

AAATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGAA

GCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTCGT

GCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGGCTG

TCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGGCGGG

GTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGCTAACCA

TGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGTGCTGGTT

ATTGTGCTGTCTCATCATTTTGGCAAAGAATTGGAGAATAAACTAGTATT

CTTCTGGTCCCCACAGACTCAGAGAGAACCCGCCACCATGTTCGTGTT

CCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGTGAACCTGACCA

CCAGGACCCAGCTGCCCCCCGCCTACACCAACAGCTTCACCAGGGGC

GTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCTGCACAGCACC

CAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTGGTTCCACGCC

ATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGACAACCCCGT

GCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGAGAAGAGCAA

CATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAGCAAGACCCA

GAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGATCAAGGTGTG

CGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTACTACCACAA

GAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTACAGCAGCG

CCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCTGATGGACC

TGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGTTCGTGTTCA

AGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACACCCCCATCAA

CCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCCTGGAGCCCCTGG

TGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGACCCTGCTGG

CCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGCAGCGGCTGG

ACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCAGCCCAGGAC

CTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGACGCCGTGGA

CTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGCACCCTGAAGAGCTT

CACCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCAGGGTGCAGCC

CACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACCTGTGCCCCTT

CGGCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGTACGCCTGGA

ACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTACA

ACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTGAGCCCCACCA

AGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTCGTGA

TCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCAGACCGGCAA

GATCGCCGACTACAACTACAAGCTGCCCGACGACTTCACCGGCTGCGT

GATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGGGCGGCAACT

ACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGAAGCCCTTCG

AGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAGCACCCCCTGC

AACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTGCAGAGCTACGGC

TTCCAGCCCACCAACGGCGTGGGCTACCAGCCCTACAGGGTGGTGGT

GCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGTGCGGCCCCA

AGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAA

CGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACAAGAAGTTCC

TGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCACCGACGCC

GTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACCCCCTGCAG

CTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACACCAGCAACC

AGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGGTGCCCGTG

GCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTGTACAGCAC

CGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGATCGGCGCCG

AGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCGGCGCCGGC

ATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCGGCAGCGCCAG

CAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAGCCTGGGCG

CCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCATCCCCACCA

ACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGAGCATGACCA

AGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACAGCACCGAGT

GCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCACCCAGCTGAACA

GGGCCCTGACCGGCATCGCCGTGGAGCAGGACAAGAACACCCAGGAG

GTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCCCCCATCAAGGAC

TTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCGACCCCAGCAAGCCC

AGCAAGAGGAGCCCCATCGAGGACCTGCTGTTCAACAAGGTGACCCTG

GCCGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCTGGGCGACAT

CGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACGGCCTGACCG

TGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAGTACACCAGCG

CCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTTCGGCGCCGGC

CCCGCCCTGCAGATCCCCTTCCCCATGCAGATGGCCTACAGGTTCAAC

GGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACCAGAAGCTGATC

GCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGGACAGCCTGAGC

AGCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGTGGTGAACCAGAA

CGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAGCAGCAACTTCG

GCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAGGCTGGACCCC

CCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCGGCAGGCTGCA

GAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGGGCCGCCGAGA

TCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAGCGAGTGCGTG

CTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGGGCTACCACCT

GATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTGTTCCTGCACG

TGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACCGCCCCCGCCA

TCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGGCGTGTTCGTG

AGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTTCTACGAGCCC

CAGATCATCACCACCGACAACACCTTCGTGAGCGGCAACTGCGACGTG

GTGATCGGCATCGTGAACAACACCGTGTACGACCCCCTGCAGCCCGAG

CTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAAGAACCACACC

AGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCAACGCCAGCGT

GGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAGGTGGCCAAGA

ACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGGCAAGTACGAG

CAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTTCATCGCCGGC

CTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTGCATGACCAGC

TGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGGCAGCTGCTGCAA

GTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGCGTGAAGCTGC

ACTACACCTGATGACTCGAGCTGGTACTGCATGCACGCAATGCTAGCT

GCCCCTTTCCCGTCCTGGGTACCCCGAGTCTCCCCCGACCTCGGGTCC

CAGGTATGCTCCCACCTCCACCTGCCCCACTCACCACCTCTGCTAGTTC

CAGACACCTCCCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTA

GCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACG

AAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCA

GCCACACCCTGGAGCTAGCAAAAAAAA

35
CTCGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCA
320

Pan-CoV
TTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAA

rAdv5-CAG-
TGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAAT

Two Proline
AATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGT

substitution
CAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAG

TGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATG

GCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACT

TGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGA

GCCCCACGTTCTGCTTCACTCTCCCCATCTCCCCCCCCTCCCCACCCC

CAATTTTGTATTTATTTATTTTTTAATTATTTTGTGCAGCGATGGGGGCG

GGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGGGGCGA

GGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCA

GAGCGGCGCGCTCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGC

GGCGGCCCTATAAAAAGCGAAGCGCGCGGCGGGCGGGAGTCGCTGC

GCGCTGCCTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCGCGCCGCC

CGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGG

GACGGCCCTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGG

CTTGTTTCTTTTCTGTGGCTGCGTGAAAGCCTTGAGGGGCTCCGGGAG

GGCCCTTTGTGCGGGGGGAGCGGCTCGGGGGGTGCGTGCGTGTGTG

TGTGCGTGGGGAGCGCCGCGTGCGGCTCCGCGCTGCCCGGCGGCTG

TGAGCGCTGCGGGCGCGGCGCGGGGCTTTGTGCGCTCCGCAGTGTGC

GCGAGGGGAGCGCGGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGG

GCTGCGAGGGGAACAAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGG

GTGAGCAGGGGGTGTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTG

CACCCCCCTCCCCGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGG

GCTCCGTACGGGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGG

TGGCGGCAGGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCC

GGGGAGGGCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGC

GGCTGTCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCG

TGCGAGAGGGCGCAGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCG

AAATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGAA

GCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTCGT

GCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGGCTG

TCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGGCGGG

GTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGCTAACCA

TGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGTGCTGGTT

ATTGTGCTGTCTCATCATTTTGGCAAAGAATTGGAGAATAAACTAGTATT

CTTCTGGTCCCCACAGACTCAGAGAGAACCCGCCACCATGTTCGTGTT

CCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGTGAACCTGACCA

CCAGGACCCAGCTGCCCCCCGCCTACACCAACAGCTTCACCAGGGGC

GTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCTGCACAGCACC

CAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTGGTTCCACGCC

ATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGACAACCCCGT

GCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGAGAAGAGCAA

CATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAGCAAGACCCA

GAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGATCAAGGTGTG

CGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTACTACCACAA

GAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTACAGCAGCG

CCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCTGATGGACC

TGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGTTCGTGTTCA

AGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACACCCCCATCAA

CCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCCTGGAGCCCCTGG

TGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGACCCTGCTGG

CCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGCAGCGGCTGG

ACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCAGCCCAGGAC

CTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGACGCCGTGGA

CTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGCACCCTGAAGAGCTT

CACCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCAGGGTGCAGCC

CACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACCTGTGCCCCTT

CGGCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGTACGCCTGGA

ACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTACA

ACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTGAGCCCCACCA

AGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTCGTGA

TCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCAGACCGGCAA

GATCGCCGACTACAACTACAAGCTGCCCGACGACTTCACCGGCTGCGT

GATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGGGCGGCAACT

ACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGAAGCCCTTCG

AGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAGCACCCCCTGC

AACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTGCAGAGCTACGGC

TTCCAGCCCACCAACGGCGTGGGCTACCAGCCCTACAGGGTGGTGGT

GCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGTGCGGCCCCA

AGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAA

CGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACAAGAAGTTCC

TGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCACCGACGCC

GTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACCCCCTGCAG

CTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACACCAGCAACC

AGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGGTGCCCGTG

GCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTGTACAGCAC

CGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGATCGGCGCCG

AGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCGGCGCCGGC

ATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCAGGAGGGCCAG

GAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAGCCTGGGCG

CCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCATCCCCACCA

ACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGAGCATGACCA

AGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACAGCACCGAGT

GCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCACCCAGCTGAACA

GGGCCCTGACCGGCATCGCCGTGGAGCAGGACAAGAACACCCAGGAG

GTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCCCCCATCAAGGAC

TTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCGACCCCAGCAAGCCC

AGCAAGAGGAGCTTCATCGAGGACCTGCTGTTCAACAAGGTGACCCTG

GCCGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCTGGGCGACAT

CGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACGGCCTGACCG

TGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAGTACACCAGCG

CCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTTCGGCGCCGGC

GCCGCCCTGCAGATCCCCTTCGCCATGCAGATGGCCTACAGGTTCAAC

GGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACCAGAAGCTGATC

GCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGGACAGCCTGAGC

AGCACCGCCAGCGCCCTGGGCAAGCTGCAGGACGTGGTGAACCAGAA

CGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAGCAGCAACTTCG

GCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAGGCTGGACCCC

CCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCGGCAGGCTGCA

GAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGGGCCGCCGAGA

TCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAGCGAGTGCGTG

CTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGGGCTACCACCT

GATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTGTTCCTGCACG

TGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACCGCCCCCGCCA

TCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGGCGTGTTCGTG

AGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTTCTACGAGCCC

CAGATCATCACCACCGACAACACCTTCGTGAGCGGCAACTGCGACGTG

GTGATCGGCATCGTGAACAACACCGTGTACGACCCCCTGCAGCCCGAG

CTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAAGAACCACACC

AGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCAACGCCAGCGT

GGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAGGTGGCCAAGA

ACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGGCAAGTACGAG

CAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTTCATCGCCGGC

CTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTGCATGACCAGC

TGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGGCAGCTGCTGCAA

GTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGCGTGAAGCTGC

ACTACACCTGATGACTCGAGCTGGTACTGCATGCACGCAATGCTAGCT

GCCCCTTTCCCGTCCTGGGTACCCCGAGTCTCCCCCGACCTCGGGTCC

CAGGTATGCTCCCACCTCCACCTGCCCCACTCACCACCTCTGCTAGTTC

CAGACACCTCCCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTA

GCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACG

AAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCA

GCCACACCCTGGAGCTAGCAAAAAAAA

36
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA
321

Pan-CoV
TGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGAC

rAdv5-CMV-
CGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT

HexaPro
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA

CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTA

CGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATG

CCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT

ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAAT

GGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCC

ATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTC

CAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGC

GTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTC

AGATCGAGAATAAACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGA

ACCCGCCACCATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAG

CCAGTGCGTGAACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACA

CCAACAGCTTCACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGA

GCAGCGTGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCA

ACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCA

AGAGGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCG

CCAGCACCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCA

CCCTGGACAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCA

ACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCC

TGGGCGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGT

TCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCC

AGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACC

TGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAG

CAAGCACACCCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAG

CGCCCTGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAG

GTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCG

ACAGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGC

TACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACC

ATCACCGACGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAA

GTGCACCCTGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAG

CAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACAT

CACCAACCTGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGC

CAGCGTGTACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCG

ACTACAGCGTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCT

ACGGCGTGAGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGT

ACGCCGACAGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCC

CCCGGCCAGACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGA

CGACTTCACCGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAG

CAAGGTGGGCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAG

CAACCTGAAGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGC

CGGCAGCACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCC

CCTGCAGAGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGC

CCTACAGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCC

ACCGTGTGCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGC

GTGAACTTCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAG

AGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCC

GACACCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGA

CATCACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCA

CCAACACCAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCA

CCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGG

AGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTG

CCTGATCGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCC

CATCGGCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCC

CCGGCAGCGCCAGCAGCGTGGCCAGCCAGAGCATCATCGCCTACACC

ATGAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCAT

CGCCATCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCC

CGTGAGCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGG

CGACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTG

CACCCAGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACA

AGAACACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCC

CCCCCATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCG

ACCCCAGCAAGCCCAGCAAGAGGAGCCCCATCGAGGACCTGCTGTTCA

ACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACT

GCCTGGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTC

AACGGCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGC

CCAGTACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGA

CCTTCGGCGCCGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATG

GCCTACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAG

AACCAGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATC

CAGGACAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGA

CGTGGTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGC

TGAGCAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGA

GCAGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATC

ACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGAT

CAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGA

TGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGC

AAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGT

GGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCAC

CACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGG

AGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGG

AACTTCTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGC

GGCAACTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGAC

CCCCTGCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTA

CTTCAAGAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCG

GCATCAACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGA

ACGAGGTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAG

CTGGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTG

GGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTG

TGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGC

GGCAGCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAA

GGGCGTGAAGCTGCACTACACCTGATGACTCGAGCTGGTACTGCATGC

ACGCAATGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCCGAGTCTCCC

CCGACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGCCCCACTCAC

CACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCAATGCAGCTC

AAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAAC

CTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTG

GTCAATTTCGTGCCAGCCACACCCTGGAGCTAGCAAAAAAAA

37
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA
322

Pan-CoV
TGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGAC

rAdv5-CMV-
CGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT

Two Proline
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA

substitution
CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTA

CGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATG

CCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT

ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAAT

GGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCC

ATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTC

CAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGC

GTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTC

AGATCGAGAATAAACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGA

ACCCGCCACCATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAG

CCAGTGCGTGAACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACA

CCAACAGCTTCACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGA

GCAGCGTGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCA

ACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCA

AGAGGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCG

CCAGCACCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCA

CCCTGGACAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCA

ACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCC

TGGGCGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGT

TCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCC

AGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACC

TGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAG

CAAGCACACCCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAG

CGCCCTGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAG

GTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCG

ACAGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGC

TACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACC

ATCACCGACGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAA

GTGCACCCTGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAG

CAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACAT

CACCAACCTGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGC

CAGCGTGTACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCG

ACTACAGCGTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCT

ACGGCGTGAGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGT

ACGCCGACAGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCC

CCCGGCCAGACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGA

CGACTTCACCGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAG

CAAGGTGGGCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAG

CAACCTGAAGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGC

CGGCAGCACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCC

CCTGCAGAGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGC

CCTACAGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCC

ACCGTGTGCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGC

GTGAACTTCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAG

AGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCC

GACACCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGA

CATCACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCA

CCAACACCAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCA

CCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGG

AGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTG

CCTGATCGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCC

CATCGGCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCC

CCAGGAGGGCCAGGAGCGTGGCCAGCCAGAGCATCATCGCCTACACC

ATGAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCAT

CGCCATCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCC

CGTGAGCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGG

CGACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTG

CACCCAGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACA

AGAACACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCC

CCCCCATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCG

ACCCCAGCAAGCCCAGCAAGAGGAGCTTCATCGAGGACCTGCTGTTCA

ACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACT

GCCTGGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTC

AACGGCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGC

CCAGTACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGA

CCTTCGGCGCCGGCGCCGCCCTGCAGATCCCCTTCGCCATGCAGATG

GCCTACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAG

AACCAGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATC

CAGGACAGCCTGAGCAGCACCGCCAGCGCCCTGGGCAAGCTGCAGGA

CGTGGTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGC

TGAGCAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGA

GCAGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATC

ACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGAT

CAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGA

TGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGC

AAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGT

GGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCAC

CACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGG

AGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGG

AACTTCTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGC

GGCAACTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGAC

CCCCTGCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTA

CTTCAAGAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCG

GCATCAACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGA

ACGAGGTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAG

CTGGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTG

GGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTG

TGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGC

GGCAGCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAA

GGGCGTGAAGCTGCACTACACCTGATGACTCGAGCTGGTACTGCATGC

ACGCAATGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCCGAGTCTCCC

CCGACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGCCCCACTCAC

CACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCAATGCAGCTC

AAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAAC

CTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTG

GTCAATTTCGTGCCAGCCACACCCTGGAGCTAGCAAAAAAAA

38
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA
323

Pan-CoV
TGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGAC

rAdv5-CMV-
CGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT

Nucleocapsid
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA

CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTA

CGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATG

CCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT

ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAAT

GGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCC

ATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTC

CAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGC

GTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTC

AGATCGAGAATAAACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGA

ACCCGCCACCATGAGCGACAACGGCCCCCAGAACCAGAGGAACGCCC

CCAGGATCACCTTCGGCGGCCCCAGCGACAGCACCGGCAGCAACCAG

AACGGCGAGAGGAGCGGCGCCAGGAGCAAGCAGAGGAGGCCCCAGG

GCCTGCCCAACAACACCGCCAGCTGGTTCACCGCCCTGACCCAGCAC

GGCAAGGAGGACCTGAAGTTCCCCAGGGGCCAGGGCGTGCCCATCAA

CACCAACAGCAGCCCCGACGACCAGATCGGCTACTACAGGAGGGCCA

CCAGGAGGATCAGGGGCGGCGACGGCAAGATGAAGGACCTGAGCCCC

AGGTGGTACTTCTACTACCTGGGCACCGGCCCCGAGGCCGGCCTGCC

CTACGGCGCCAACAAGGACGGCATCATCTGGGTGGCCACCGAGGGCG

CCCTGAACACCCCCAAGGACCACATCGGCACCAGGAACCCCGCCAACA

ACGCCGCCATCGTGCTGCAGCTGCCCCAGGGCACCACCCTGCCCAAG

GGCTTCTACGCCGAGGGCAGCAGGGGCGGCAGCCAGGCCAGCAGCA

GGAGCAGCAGCAGGAGCAGGAACAGCAGCAGGAACAGCACCCCCGGC

AGCAGCAGGGGCACCAGCCCCGCCAGGATGGCCGGCAACGGCGGCG

ACGCCGCCCTGGCCCTGCTGCTGCTGGACAGGCTGAACCAGCTGGAG

AGCAAGATGAGCGGCAAGGGCCAGCAGCAGCAGGGCCAGACCGTGAC

CAAGAAGAGCGCCGCCGAGGCCAGCAAGAAGCCCAGGCAGAAGAGGA

CCGCCACCAAGGCCTACAACGTGACCCAGGCCTTCGGCAGGAGGGGC

CCCGAGCAGACCCAGGGCAACTTCGGCGACCAGGAGCTGATCAGGCA

GGGCACCGACTACAAGCACTGGCCCCAGATCGCCCAGTTCGCCCCCA

GCGCCAGCGCCTTCTTCGGCATGAGCAGGATCGGCATGGAGGTGACC

CCCAGCGGCACCTGGCTGACCTACACCGGCGCCATCAAGCTGGACGA

CAAGGACCCCAACTTCAAGGACCAGGTGATCCTGCTGAACAAGCACAT

CGACGCCTACAAGACCTTCCCCCCCACCGAGCCCAAGAAGGACAAGAA

GAAGAAGGCCGACGAGACCCAGGCCCTGCCCCAGAGGCAGAAGAAGC

AGCAGACCGTGACCCTGCTGCCCGCCGCCGACCTGGACGACTTCAGC

AAGCAGCTGCAGCAGAGCATGAGCAGCGCCGACAGCACCCAGGCCTG

ATGACTCGAGCTGGTACTGCATGCACGCAATGCTAGCTGCCCCTTTCC

CGTCCTGGGTACCCCGAGTCTCCCCCGACCTCGGGTCCCAGGTATGCT

CCCACCTCCACCTGCCCCACTCACCACCTCTGCTAGTTCCAGACACCT

CCCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCC

CCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC

TAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACACCCT

GGAGCTAGCAAAAAAAA

39
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA
324

Pan-CoV
TGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGAC

rAdv5-CMV-
CGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT

ORF1a/b
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA

CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTA

CGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATG

CCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT

ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAAT

GGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCC

ATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTC

CAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGC

GTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTC

AGATCGAGAATAAACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGA

ACCCGCCACCATGGACCAGGAACCCCGCCTGGAGGAAGGCCGTGTTC

ATCAGCCCCTACAACAGCCAGAACGCCGTGGCCAGCAAGATCCTGGGC

CTGCCCACCCAGACCGTGGACAGCAGCCAGGGCAGCGAGTACGACTA

CGTGATCTTCACCCAGACCACCGAGACCGCCCACAGCTGCAACGTGAA

CAGGTTCAACGTGGCCATCACCAGGGCCAAGGTGGGCATCCTGTGCAT

CATGAGCGACAGGGACCTGTACGACAAGCTGCAGTTCACCAGCCTGGA

GATCCCCAGGAGGAACGTGGCCACCCTGCAGGCCGAGAACGTGACCG

GCCTGTTCAAGGACTGCAGCAAGGTGATCACCGGCCTGCACCCCACCC

AGGCCCCCACCCACCTGAGCGTGGACACCAAGTTCAAGACCGAGGGC

CTGTGCGTGGACATCCCCGGCATCCCCAAGGACATGACCTACAGGAGG

CTGATCAGCATGATGGGCTTCAAGATGAACTACCAGGTGAACGGCTAC

CCCAACATGTTCATCACCAGGGAGGAGGCCATCAGGCACGTGAGGGC

CTGGATCGGCTTCGACGTGGAGGGCTGCCACGCCACCAGGGAGGCCG

TGGGCACCAACCTGCCCCTGCAGCTGGGCTTCAGCACCGGCGTGAAC

CTGGTGGCCGTGCCCACCGGCTACGTGGACACCCCCAACAACACCGA

CTTCAGCAGGGTGAGCGCCAAGCCCCCCCCCGGCGACCAGTTCAAGC

ACCTGATCCCCCTGATGTACAAGGGCCTGCCCTGGAACGTGGTGAGGA

TCAAGATCGTGCAGATGCTGAGCGACACCCTGAAGAACCTGAGCGACA

GGGTGGTGTTCGTGCTGTGGGCCCACGGCTTCGAGCTGACCAGCATG

AAGTACTTCGTGAAGATCGGCCCCGAGAGGACCTGCTGCCTGTGCGAC

AGGAGGGCCACCTGCTTCAGCACCGCCAGCGACACCTACGCCTGCTG

GCACCACAGCATCGGCTTCGACTACGTGTACAACCCCTTCATGATCGA

CGTGCAGCAGTGGGGCTTCACCGGCAACCTGCAGAGCAACCACGACC

TGTACTGCCAGGTGCACGGCAACGCCCACGTGGCCAGCTGCGACGCC

ATCATGACCAGGTGCCTGGCCGTGCACGAGTGCTTCGTGAAGAGGGTG

GACTGGACCATCGAGTACCCCATCATCGGCGACGAGCTGAAGATCAAC

GCCGCCTGCAGGAAGGTGCAGCACATGGTGGTGAAGGCCGCCCTGCT

GGCCGACAAGTTCCCCGTGCTGCACGACATCGGCAACCCCAAGGCCAT

CAAGTGCGTGCCCCAGGCCGACGTGGAGTGGAAGTTCTACGACGCCC

AGCCCTGCAGCGACAAGGCCTACAAGATCGAGGAGCTGTTCTACAGCT

ACGCCACCCACAGCGACAAGTTCACCGACGGCGTGTGCCTGTTCTGGA

ACTGCAACGTGGACAGGTACCCCGCCAACAGCATCGTGTGCAGGTTCG

ACACCAGGGTGCTGAGCAACCTGAACCTGCCCGGCTGCGACGGCGGC

AGCCTGTACGTGAACAAGCACGCCTTCCACACCCCCGCCTTCGACAAG

AGCGCCTTCGTGAACCTGAAGCAGCTGCCCTTCTTCTACTACAGCGAC

AGCCCCTGCGAGAGCCACGGCAAGCAGGTGGTGAGCGACATCGACTA

CGTGCCCCTGAAGAGCGCCACCTGCATCACCAGGTGCAACCTGGGCG

GCGCCGTGTGCAGGCACCACGCCAACGAGTACAGGCTGTACCTGGAC

GCCTACAACATGATGATCAGCGCCGGCTTCAGCCTGTGGGTGTACAAG

CAGTTCGACACCTACAACCTGTGGAACACCTTCACCAGGCTGCAGAGC

CTGGAGAACGTGGCCTTCAACGTGGTGAACAAGGGCCACTTCGACGGC

CAGCAGGGCGAGGTGCCCGTGAGCATCATCAACAACACCGTGTACACC

AAGGTGGACGGCGTGGACGTGGAGCTGTTCGAGAACAAGACCACCCT

GCCCGTGAACGTGGCCTTCGAGCTGTGGGCCAAGAGGAACATCAAGC

CCGTGCCCGAGGTGAAGATCCTGAACAACCTGGGCGTGGACATCGCC

GCCAACACCGTGATCTGGGACTACAAGAGGGACGCCCCCGCCCACAT

CAGCACCATCGGCGTGTGCAGCATGACCGACATCGCCAAGAAGCCCAC

CGAGACCATCTGCGCCCCCCTGACCGTGTTCTTCGACGGCAGGGTGG

ACGGCCAGGTGGACCTGTTCAGGAACGCCAGGAACGGCGTGCTGATC

ACCGAGGGCAGCGTGAAGGGCCTGCAGCCCAGCGTGGGCCCCAAGCA

GGCCAGCCTGAACGGCGTGACCCTGATCGGCGAGGCCGTGAAGACCC

AGTTCAACTACTACAAGAAGGTGGACGGCGTGGTGCAGCAGCTGCCCG

AGACCTACTTCACCCAGAGCAGGAACCTGCAGGAGTTCAAGCCCAGGA

GCCAGATGGAGATCGACTTCCTGGAGCTGGCCATGGACGAGTTCATCG

AGAGGTACAAGCTGGAGGGCTACGCCTTCGAGCACATCGTGTACGGC

GACTTCAGCCACAGCCAGCTGGGCGGCCTGCACCTGCTGATCGGCCT

GGCCAAGAGGTTCAAGGAGAGCCCCTTCGAGCTGGAGGACTTCATCCC

CATGGACAGCACCGTGAAGAACTACTTCATCACCGACGCCCAGACCGG

CAGCAGCAAGTGCGTGTGCAGCGTGATCGACCTGCTGCTGGACGACTT

CGTGGAGATCATCAAGAGCCAGGACCTGAGCGTGGTGAGCAAGGTGG

TGAAGGTGACCATCGACTACACCGAGATCAGCTTCATGCTGTGGTGCA

AGGACGGCCACGTGGAGACCTTCTACCCCAAGCTGCAGAGCAGCCAG

GCCTGGCAGCCCGGCGTGGCCATGCCCAACCTGTACAAGATGCAGAG

GATGCTGCTGGAGAAGTGCGACCTGCAGAACTACGGCGACAGCGCCA

CCCTGCCCAAGGGCATCATGATGAACGTGGCCAAGTACACCCAGCTGT

GCCAGTACCTGAACACCCTGACCCTGGCCGTGCCCTACAACATGAGGG

TGATCCACTTCGGCGCCGGCAGCGACAAGGGCGTGGCCCCCGGCACC

GCCGTGCTGAGGCAGTGGCTGCCCACCGGCACCCTGCTGGTGGACAG

CGACCTGAACGACTTCGTGAGCGACGCCGACAGCACCCTGATCGGCG

ACTGCGCCACCGTGCACACCGCCAACAAGTGGGACCTGATCATCAGCG

ACATGTACGACCCCAAGACCAAGAACGTGACCAAGGAGAACGACAGCA

AGGAGGGCTTCTTCACCTACATCTGCGGCTTCATCCAGCAGAAGCTGG

CCCTGGGCGGCAGCGTGGCCATCAAGATCACCGAGCACAGCTGGAAC

GCCGACCTGTACAAGCTGATGGGCCACTTCGCCTGGTGGACCGCCTTC

GTGACCAACGTGAACGCCAGCAGCAGCGAGGCCTTCCTGATCGGCTG

CAACTACCTGGGCAAGCCCAGGGAGCAGATCGACGGCTACGTGATGC

ACGCCAACTACATCTTCTGGAGGAACACCAACCCCATCCAGCTGAGCA

GCTACAGCCTGTTCGACATGAGCAAGTTCCCCCTGAAGCTTGATGACTC

GAGCTGGTACTGCATGCACGCAATGCTAGCTGCCCCTTTCCCGTCCTG

GGTACCCCGAGTCTCCCCCGACCTCGGGTCCCAGGTATGCTCCCACCT

CCACCTGCCCCACTCACCACCTCTGCTAGTTCCAGACACCTCCCAAGC

ACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGG

AAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTAT

ACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACACCCTGGAGCTA

GCAAAAAAAA

40
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA
325

Pan-CoV
TGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGAC

rAdv5-CMV-
CGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT

Envelope
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA

CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTA

CGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATG

CCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT

ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAAT

GGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCC

ATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTC

CAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGC

GTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTC

AGATCGAGAATAAACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGA

ACCCGCCACCATGTACAGCTTCGTGAGCGAGGAGACCGGCACCCTGAT

CGTGAACAGCGTGCTGCTGTTCCTGGCCTTCGTGGTGTTCCTGCTGGT

GACCCTGGCCATCCTGACCGCCCTGAGGCTGTGCGCCTACTGCTGCAA

CATCGTGAACGTGAGCCTGGTGAAGCCCAGCTTCTACGTGTACAGCAG

GGTGAAGAACCTGAACAGCAGCAGGGTGCCCGACCTGCTGGTGTGAT

GACTCGAGCTGGTACTGCATGCACGCAATGCTAGCTGCCCCTTTCCCG

TCCTGGGTACCCCGAGTCTCCCCCGACCTCGGGTCCCAGGTATGCTCC

CACCTCCACCTGCCCCACTCACCACCTCTGCTAGTTCCAGACACCTCC

CAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCC

ACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTA

AGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACACCCTG

GAGCTAGCAAAAAAAA

41
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA
326

Pan-CoV
TGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGAC

rAdv5-CMV-
CGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT

Membrane
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA

CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTA

CGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATG

CCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT

ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAAT

GGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCC

ATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTC

CAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGC

GTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTC

AGATCGAGAATAAACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGA

ACCCGCCACCATGGCCGACAGCAACGGCACCATCACCGTGGAGGAGC

TGAAGAAGCTGCTGGAGCAGTGGAACCTGGTGATCGGCTTCCTGTTCC

TGACCTGGATCTGCCTGCTGCAGTTCGCCTACGCCAACAGGAACAGGT

TCCTGTACATCATCAAGCTGATCTTCCTGTGGCTGCTGTGGCCCGTGAC

CCTGGCCTGCTTCGTGCTGGCCGCCGTGTACAGGATCAACTGGATCAC

CGGCGGCATCGCCATCGCCATGGCCTGCCTGGTGGGCCTGATGTGGC

TGAGCTACTTCATCGCCAGCTTCAGGCTGTTCGCCAGGACCAGGAGCA

TGTGGAGCTTCAACCCCGAGACCAACATCCTGCTGAACGTGCCCCTGC

ACGGCACCATCCTGACCAGGCCCCTGCTGGAGAGCGAGCTGGTGATC

GGCGCCGTGATCCTGAGGGGCCACCTGAGGATCGCCGGCCACCACCT

GGGCAGGTGCGACATCAAGGACCTGCCCAAGGAGATCACCGTGGCCA

CCAGCAGGACCCTGAGCTACTACAAGCTGGGCGCCAGCCAGAGGGTG

GCCGGCGACAGCGGCTTCGCCGCCTACAGCAGGTACAGGATCGGCAA

CTACAAGCTGAACACCGACCACAGCAGCAGCAGCGACAACATCGCCCT

GCTGGTGCAGTGATGACTCGAGCTGGTACTGCATGCACGCAATGCTAG

CTGCCCCTTTCCCGTCCTGGGTACCCCGAGTCTCCCCCGACCTCGGGT

CCCAGGTATGCTCCCACCTCCACCTGCCCCACTCACCACCTCTGCTAG

TTCCAGACACCTCCCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGC

CTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAA

CGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGC

CAGCCACACCCTGGAGCTAGCAAAAAAAA

42
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA
327

Pan-CoV
TGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGAC

rAdv5-CMV-
CGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT

ORF7a
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA

CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTA

CGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATG

CCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT

ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAAT

GGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCC

ATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTC

CAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGC

GTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTC

AGATCGAGAATAAACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGA

ACCCGCCACCATGAAGATCATCCTGTTCCTGGCCCTGATCACCCTGGC

CACCTGCGAGCTGTACCACTACCAGGAGTGCGTGAGGGGCACCACCG

TGTGATGACTCGAGCTGGTACTGCATGCACGCAATGCTAGCTGCCCCT

TTCCCGTCCTGGGTACCCCGAGTCTCCCCCGACCTCGGGTCCCAGGTA

TGCTCCCACCTCCACCTGCCCCACTCACCACCTCTGCTAGTTCCAGACA

CCTCCCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACA

CCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTT

AACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACAC

CCTGGAGCTAGCAAAAAAAA

43
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA
328

Pan-CoV
TGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGAC

rAdv5-CMV-
CGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT

NSP3
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA

CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTA

CGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATG

CCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT

ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAAT

GGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCC

ATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTC

CAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGC

GTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTC

AGATCGAGAATAAACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGA

ACCCGCCACCATGGCCCCCACCAAGGTGACCTTCGGCGACGACACCG

TGATCGAGGTGCAGGGCTACAAGAGCGTGAACATCACCTTCGAGCTGG

ACGAGAGGATCGACAAGGTGCTGAACGAGAAGTGCAGCGCCTACACC

GTGGAGCTGGGCACCGAGGTGAACGAGTTCGCCTGCGTGGTGGCCGA

CGCCGTGATCAAGACCCTGCAGCCCGTGAGCGAGCTGCTGACCCCCC

TGGGCATCGACCTGGACGAGTGGAGCATGGCCACCTACTACCTGTTCG

ACGAGAGCGGCGAGTTCAAGCTGGCCAGCCACATGTACTGCAGCTTCT

ACCCCCCCGACGAGGACGAGGAGGAGGGCGACTGCGAGGAGGAGGA

GTTCGAGCCCAGCACCCAGTACGAGTACGGCACCGAGGACGACTACC

AGGGCAAGCCCCTGGAGTTCGGCGCCACCAGCGCCGCCCTGCAGCCC

GAGGAGGAGCAGGAGGAGGACTGGCTGGACGACGACAGCCAGCAGA

CCGTGGGCCAGCAGGACGGCAGCGAGGACAACCAGACCACCACCATC

CAGACCATCGTGGAGGTGCAGCCCCAGCTGGAGATGGAGCTGACCCC

CGTGGTGCAGACCATCGAGGTGAACAGCTTCAGCGGCTACCTGAAGCT

GACCGACAACGTGTACATCAAGAACGCCGACATCGTGGAGGAGGCCAA

GAAGGTGAAGCCCACCGTGGTGGTGAACGCCGCCAACGTGTACCTGA

AGCACGGCGGCGGCGTGGCCGGCGCCCTGAACAAGGCCACCAACAAC

GCCATGCAGGTGGAGAGCGACGACTACATCGCCACCAACGGCCCCCT

GAAGGTGGGCGGCAGCTGCGTGCTGAGCGGCCACAACCTGGCCAAGC

ACTGCCTGCACGTGGTGGGCCCCAACGTGAACAAGGGCGAGGACATC

CAGCTGCTGAAGAGCGCCTACGAGAACTTCAACCAGCACGAGGTGCTG

CTGGCCCCCCTGCTGAGCGCCGGCATCTTCGGCGCCGACCCCATCCA

CAGCCTGAGGGTGTGCGTGGACACCGTGAGGACCAACGTGTACCTGG

CCGTGTTCGACAAGAACCTGTACGACAAGCTGGTGAGCAGCTTCCTGG

AGATGAAGAGCGAGAAGCAGGTGGAGCAGAAGATCGCCGAGATCCCC

AAGGAGGAGGTGAAGCCCTTCATCACCGAGAGCAAGCCCAGCGTGGA

GCAGAGGAAGCAGGACGACAAGAAGATCAAGGCCTGCGTGGAGGAGG

TGACCACCACCCTGGAGGAGACCAAGTTCCTGACCGAGAACCTGCTGC

TGTACATCGACATCAACGGCAACCTGCACCCCGACAGCGCCACCCTGG

TGAGCGACATCGACATCACCTTCCTGAAGAAGGACGCCCCCTACATCG

TGGGCGACGTGGTGCAGGAGGGCGTGCTGACCGCCGTGGTGATCCCC

ACCAAGAAGGCCGGCGGCACCACCGAGATGCTGGCCAAGGCCCTGAG

GAAGGTGCCCACCGACAACTACATCACCACCTACCCCGGCCAGGGCCT

GAACGGCTACACCGTGGAGGAGGCCAAGACCGTGCTGAAGAAGTGCA

AGAGCGCCTTCTACATCCTGCCCAGCATCATCAGCAACGAGAAGCAGG

AGATCCTGGGCACCGTGAGCTGGAACCTGAGGGAGATGCTGGCCCAC

GCCGAGGAGACCAGGAAGCTGATGCCCGTGTGCGTGGAGACCAAGGC

CATCGTGAGCACCATCCAGAGGAAGTACAAGGGCATCAAGATCCAGGA

GGGCGTGGTGGACTACGGCGCCAGGTTCTACTTCTACACCAGCAAGAC

CACCGTGGCCAGCCTGATCAACACCCTGAACGACCTGAACGAGACCCT

GGTGACCATGCCCCTGGGCTACGTGACCCACGGCCTGAACCTGGAGG

AGGCCGCCAGGTACATGAGGAGCCTGAAGGTGCCCGCCACCGTGAGC

GTGAGCAGCCCCGACGCCGTGACCGCCTACAACGGCTACCTGACCAG

CAGCAGCAAGACCCCCGAGGAGCACTTCATCGAGACCATCAGCCTGGC

CGGCAGCTACAAGGACTGGAGCTACAGCGGCCAGAGCACCCAGCTGG

GCATCGAGTTCCTGAAGAGGGGCGACAAGAGCGTGTACTACACCAGCA

ACCCCACCACCTTCCACCTGGACGGCGAGGTGATCACCTTCGACAACC

TGAAGACCCTGCTGAGCCTGAGGGAGGTGAGGACCATCAAGGTGTTCA

CCACCGTGGACAACATCAACCTGCACTGATGACTCGAGCTGGTACTGC

ATGCACGCAATGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCCGAGTC

TCCCCCGACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGCCCCAC

TCACCACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCAATGCA

GCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGAT

TAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGG

TTGGTCAATTTCGTGCCAGCCACACCCTGGAGCTAGCAAAAAAAA

44
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA
329

Pan-CoV
TGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGAC

rAdv5-CMV-
CGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT

NSP5
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA

CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTA

CGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATG

CCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT

ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAAT

GGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCC

ATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTC

CAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGC

GTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTC

AGATCGAGAATAAACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGA

ACCCGCCACCATGAGCGGCTTCAGGAAGATGGCCTTCCCCAGCGGCA

AGGTGGAGGGCTGCATGGTGCAGGTGACCTGCGGCACCACCACCCTG

AACGGCCTGTGGCTGGACGACGTGGTGTACTGCCCCAGGCACGTGAT

CTGCACCAGCGAGGACATGCTGAACCCCAACTACGAGGACCTGCTGAT

CAGGAAGAGCAACCACAACTTCCTGGTGCAGGCCGGCAACGTGCAGCT

GAGGGTGATCGGCCACAGCATGCAGAACTGCGTGCTGAAGCTGAAGG

TGGACACCGCCAACCCCAAGACCCCCAAGTACAAGTTCGTGAGGATCC

AGCCCGGCCAGACCTTCAGCGTGCTGGCCTGCTACAACGGCAGCCCC

AGCGGCGTGTACCAGTGCGCCATGAGGCCCAACTTCACCATCAAGGGC

AGCTTCCTGAACGGCAGCTGCGGCAGCGTGGGCTTCAACATCGACTAC

GACTGCGTGAGCTTCTGCTACATGCACCACATGGAGCTGCCCACCGGC

GTGCACGCCGGCACCGACCTGGAGGGCAACTTCTACGGCCCCTTCGT

GGACAGGCAGACCGCCCAGGCCGCCGGCACCGACACCACCATCACCG

TGAACGTGCTGGCCTGGCTGTACGCCGCCGTGATCAACGGCGACAGG

TGGTTCCTGAACAGGTTCACCACCACCCTGAACGACTTCAACCTGGTG

GCCATGAAGTACAACTACGAGCCCCTGACCCAGGACCACGTGGACATC

CTGGGCCCCCTGAGCGCCCAGACCGGCATCGCCGTGCTGGACATGTG

CGCCAGCCTGAAGGAGCTGCTGCAGAACGGCATGAACGGCAGGACCA

TCCTGGGCAGCGCCCTGCTGGAGGACGAGTTCACCCCCTTCGACGTG

GTGAGGCAGTGCAGCGGCGTGACCTTCCAGTGATGACTCGAGCTGGTA

CTGCATGCACGCAATGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCCG

AGTCTCCCCCGACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGCC

CCACTCACCACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCAA

TGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAG

TGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCC

AGGGTTGGTCAATTTCGTGCCAGCCACACCCTGGAGCTAGCAAAAAAA

A

45
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA
330

Pan-CoV
TGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGAC

rAdv5-CMV-
CGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT

NSP12
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA

CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTA

CGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATG

CCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT

ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAAT

GGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCC

ATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTC

CAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGC

GTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTC

AGATCGAGAATAAACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGA

ACCCGCCACCATGAGCGCCGACGCCCAGAGCTTCCTGAACAGGGTGT

GCGGCGTGAGCGCCGCCAGGCTGACCCCCTGCGGCACCGGCACCAG

CACCGACGTGGTGTACAGGGCCTTCGACATCTACAACGACAAGGTGGC

CGGCTTCGCCAAGTTCCTGAAGACCAACTGCTGCAGGTTCCAGGAGAA

GGACGAGGACGACAACCTGATCGACAGCTACTTCGTGGTGAAGAGGCA

CACCTTCAGCAACTACCAGCACGAGGAGACCATCTACAACCTGCTGAA

GGACTGCCCCGCCGTGGCCAAGCACGACTTCTTCAAGTTCAGGATCGA

CGGCGACATGGTGCCCCACATCAGCAGGCAGAGGCTGACCAAGTACA

CCATGGCCGACCTGGTGTACGCCCTGAGGCACTTCGACGAGGGCAAC

TGCGACACCCTGAAGGAGATCCTGGTGACCTACAACTGCTGCGACGAC

GACTACTTCAACAAGAAGGACTGGTACGACTTCGTGGAGAACCCCGAC

ATCCTGAGGGTGTACGCCAACCTGGGCGAGAGGGTGAGGCAGGCCCT

GCTGAAGACCGTGCAGTTCTGCGACGCCATGAGGAACGCCGGCATCG

TGGGCGTGCTGACCCTGGACAACCAGGACCTGAACGGCAACTGGTAC

GACTTCGGCGACTTCATCCAGACCACCCCCGGCAGCGGCGTGCCCGT

GGTGGACAGCTACTACAGCCTGCTGATGCCCATCCTGACCCTGACCAG

GGCCCTGACCGCCGAGAGCCACGTGGACACCGACCTGACCAAGCCCT

ACATCAAGTGGGACCTGCTGAAGTACGACTTCACCGAGGAGAGGCTGA

AGCTGTTCGACAGGTACTTCAAGTACTGGGACCAGACCTACCACCCCA

ACTGCGTGAACTGCCTGGACGACAGGTGCATCCTGCACTGCGCCAACT

TCAACGTGCTGTTCAGCACCGTGTTCCCCCCCACCAGCTTCGGCCCCC

TGGTGAGGAAGATCTTCGTGGACGGCGTGCCCTTCGTGGTGAGCACC

GGCTACCACTTCAGGGAGCTGGGCGTGGTGCACAACCAGGACGTGAA

CCTGCACAGCAGCAGGCTGAGCTTCAAGGAGCTGCTGGTGTACGCCG

CCGACCCCGCCATGCACGCCGCCAGCGGCAACCTGCTGCTGGACAAG

AGGACCACCTGCTTCAGCGTGGCCGCCCTGACCAACAACGTGGCCTTC

CAGACCGTGAAGCCCGGCAACTTCAACAAGGACTTCTACGACTTCGCC

GTGAGCAAGGGCTTCTTCAAGGAGGGCAGCAGCGTGGAGCTGAAGCA

CTTCTTCTTCGCCCAGGACGGCAACGCCGCCATCAGCGACTACGACTA

CTACAGGTACAACCTGCCCACCATGTGCGACATCAGGCAGCTGCTGTT

CGTGGTGGAGGTGGTGGACAAGTACTTCGACTGCTACGACGGCGGCT

GCATCAACGCCAACCAGGTGATCGTGAACAACCTGGACAAGAGCGCCG

GCTTCCCCTTCAACAAGTGGGGCAAGGCCAGGCTGTACTACGACAGCA

TGAGCTACGAGGACCAGGACGCCCTGTTCGCCTACACCAAGAGGAACG

TGATCCCCACCATCACCCAGATGAACCTGAAGTACGCCATCAGCGCCA

AGAACAGGGCCAGGACCGTGGCCGGCGTGAGCATCTGCAGCACCATG

ACCAACAGGCAGTTCCACCAGAAGCTGCTGAAGAGCATCGCCGCCACC

AGGGGCGCCACCGTGGTGATCGGCACCAGCAAGTTCTACGGCGGCTG

GCACAACATGCTGAAGACCGTGTACAGCGACGTGGAGAACCCCCACCT

GATGGGCTGGGACTACCCCAAGTGCGACAGGGCCATGCCCAACATGC

TGAGGATCATGGCCAGCCTGGTGCTGGCCAGGAAGCACACCACCTGCT

GCAGCCTGAGCCACAGGTTCTACAGGCTGGCCAACGAGTGCGCCCAG

GTGCTGAGCGAGATGGTGATGTGCGGCGGCAGCCTGTACGTGAAGCC

CGGCGGCACCAGCAGCGGCGACGCCACCACCGCCTACGCCAACAGCG

TGTTCAACATCTGCCAGGCCGTGACCGCCAACGTGAACGCCCTGCTGA

GCACCGACGGCAACAAGATCGCCGACAAGTACGTGAGGAACCTGCAG

CACAGGCTGTACGAGTGCCTGTACAGGAACAGGGACGTGGACACCGA

CTTCGTGAACGAGTTCTACGCCTACCTGAGGAAGCACTTCAGCATGATG

ATCCTGAGCGACGACGCCGTGGTGTGCTTCAACAGCACCTACGCCAGC

CAGGGCCTGGTGGCCAGCATCAAGAACTTCAAGAGCGTGCTGTACTAC

CAGAACAACGTGTTCATGAGCGAGGCCAAGTGCTGGACCGAGACCGAC

CTGACCAAGGGCCCCCACGAGTTCTGCAGCCAGCACACCATGCTGGTG

AAGCAGGGCGACGACTACGTGTACCTGCCCTACCCCGACCCCAGCAG

GATCCTGGGCGCCGGCTGCTTCGTGGACGACATCGTGAAGACCGACG

GCACCCTGATGATCGAGAGGTTCGTGAGCCTGGCCATCGACGCCTACC

CCCTGACCAAGCACCCCAACCAGGAGTACGCCGACGTGTTCCACCTGT

ACCTGCAGTACATCAGGAAGCTGCACGACGAGCTGACCGGCCACATGC

TGGACATGTACAGCGTGATGCTGACCAACGACAACACCAGCAGGTACT

GGGAGCCCGAGTTCTACGAGGCCATGTACACCCCCCACACCGTGCTG

CAGTGATGACTCGAGCTGGTACTGCATGCACGCAATGCTAGCTGCCCC

TTTCCCGTCCTGGGTACCCCGAGTCTCCCCCGACCTCGGGTCCCAGGT

ATGCTCCCACCTCCACCTGCCCCACTCACCACCTCTGCTAGTTCCAGAC

ACCTCCCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCAC

ACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT

TAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACA

CCCTGGAGCTAGCAAAAAAAA

46
CTCGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCA
331

Pan-CoV
TTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAA

rAdv5-CAG-
TGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAAT

Nucleocapsid
AATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGT

CAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAG

TGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATG

GCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACT

TGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGA

GCCCCACGTTCTGCTTCACTCTCCCCATCTCCCCCCCCTCCCCACCCC

CAATTTTGTATTTATTTATTTTTTAATTATTTTGTGCAGCGATGGGGGCG

GGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGGGGCGA

GGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCA

GAGCGGCGCGCTCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGC

GGCGGCCCTATAAAAAGCGAAGCGCGCGGCGGGCGGGAGTCGCTGC

GCGCTGCCTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCGCGCCGCC

CGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGG

GACGGCCCTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGG

CTTGTTTCTTTTCTGTGGCTGCGTGAAAGCCTTGAGGGGCTCCGGGAG

GGCCCTTTGTGCGGGGGGAGCGGCTCGGGGGGTGCGTGCGTGTGTG

TGTGCGTGGGGAGCGCCGCGTGCGGCTCCGCGCTGCCCGGCGGCTG

TGAGCGCTGCGGGCGCGGCGCGGGGCTTTGTGCGCTCCGCAGTGTGC

GCGAGGGGAGCGCGGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGG

GCTGCGAGGGGAACAAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGG

GTGAGCAGGGGGTGTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTG

CACCCCCCTCCCCGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGG

GCTCCGTACGGGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGG

TGGCGGCAGGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCC

GGGGAGGGCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGC

GGCTGTCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCG

TGCGAGAGGGCGCAGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCG

AAATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGAA

GCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTCGT

GCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGGCTG

TCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGGCGGG

GTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGCTAACCA

TGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGTGCTGGTT

ATTGTGCTGTCTCATCATTTTGGCAAAGAATTGGAGAATAAACTAGTATT

CTTCTGGTCCCCACAGACTCAGAGAGAACCCGCCACCATGAGCGACAA

CGGCCCCCAGAACCAGAGGAACGCCCCCAGGATCACCTTCGGCGGCC

CCAGCGACAGCACCGGCAGCAACCAGAACGGCGAGAGGAGCGGCGC

CAGGAGCAAGCAGAGGAGGCCCCAGGGCCTGCCCAACAACACCGCCA

GCTGGTTCACCGCCCTGACCCAGCACGGCAAGGAGGACCTGAAGTTC

CCCAGGGGCCAGGGCGTGCCCATCAACACCAACAGCAGCCCCGACGA

CCAGATCGGCTACTACAGGAGGGCCACCAGGAGGATCAGGGGCGGCG

ACGGCAAGATGAAGGACCTGAGCCCCAGGTGGTACTTCTACTACCTGG

GCACCGGCCCCGAGGCCGGCCTGCCCTACGGCGCCAACAAGGACGG

CATCATCTGGGTGGCCACCGAGGGCGCCCTGAACACCCCCAAGGACC

ACATCGGCACCAGGAACCCCGCCAACAACGCCGCCATCGTGCTGCAG

CTGCCCCAGGGCACCACCCTGCCCAAGGGCTTCTACGCCGAGGGCAG

CAGGGGCGGCAGCCAGGCCAGCAGCAGGAGCAGCAGCAGGAGCAGG

AACAGCAGCAGGAACAGCACCCCCGGCAGCAGCAGGGGCACCAGCCC

CGCCAGGATGGCCGGCAACGGCGGCGACGCCGCCCTGGCCCTGCTG

CTGCTGGACAGGCTGAACCAGCTGGAGAGCAAGATGAGCGGCAAGGG

CCAGCAGCAGCAGGGCCAGACCGTGACCAAGAAGAGCGCCGCCGAGG

CCAGCAAGAAGCCCAGGCAGAAGAGGACCGCCACCAAGGCCTACAAC

GTGACCCAGGCCTTCGGCAGGAGGGGCCCCGAGCAGACCCAGGGCAA

CTTCGGCGACCAGGAGCTGATCAGGCAGGGCACCGACTACAAGCACT

GGCCCCAGATCGCCCAGTTCGCCCCCAGCGCCAGCGCCTTCTTCGGC

ATGAGCAGGATCGGCATGGAGGTGACCCCCAGCGGCACCTGGCTGAC

CTACACCGGCGCCATCAAGCTGGACGACAAGGACCCCAACTTCAAGGA

CCAGGTGATCCTGCTGAACAAGCACATCGACGCCTACAAGACCTTCCC

CCCCACCGAGCCCAAGAAGGACAAGAAGAAGAAGGCCGACGAGACCC

AGGCCCTGCCCCAGAGGCAGAAGAAGCAGCAGACCGTGACCCTGCTG

CCCGCCGCCGACCTGGACGACTTCAGCAAGCAGCTGCAGCAGAGCAT

GAGCAGCGCCGACAGCACCCAGGCCTGATGACTCGAGCTGGTACTGC

ATGCACGCAATGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCCGAGTC

TCCCCCGACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGCCCCAC

TCACCACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCAATGCA

GCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGAT

TAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGG

TTGGTCAATTTCGTGCCAGCCACACCCTGGAGCTAGCAAAAAAAA

47
CTCGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCA
332

Pan-CoV
TTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAA

rAdv5-CAG-
TGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAAT

ORF1a/b
AATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGT

CAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAG

TGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATG

GCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACT

TGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGA

GCCCCACGTTCTGCTTCACTCTCCCCATCTCCCCCCCCTCCCCACCCC

CAATTTTGTATTTATTTATTTTTTAATTATTTTGTGCAGCGATGGGGGCG

GGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGGGGCGA

GGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCA

GAGCGGCGCGCTCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGC

GGCGGCCCTATAAAAAGCGAAGCGCGCGGCGGGCGGGAGTCGCTGC

GCGCTGCCTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCGCGCCGCC

CGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGG

GACGGCCCTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGG

CTTGTTTCTTTTCTGTGGCTGCGTGAAAGCCTTGAGGGGCTCCGGGAG

GGCCCTTTGTGCGGGGGGAGCGGCTCGGGGGGTGCGTGCGTGTGTG

TGTGCGTGGGGAGCGCCGCGTGCGGCTCCGCGCTGCCCGGCGGCTG

TGAGCGCTGCGGGCGCGGCGCGGGGCTTTGTGCGCTCCGCAGTGTGC

GCGAGGGGAGCGCGGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGG

GCTGCGAGGGGAACAAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGG

GTGAGCAGGGGGTGTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTG

CACCCCCCTCCCCGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGG

GCTCCGTACGGGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGG

TGGCGGCAGGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCC

GGGGAGGGCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGC

GGCTGTCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCG

TGCGAGAGGGCGCAGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCG

AAATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGAA

GCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTCGT

GCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGGCTG

TCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGGCGGG

GTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGCTAACCA

TGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGTGCTGGTT

ATTGTGCTGTCTCATCATTTTGGCAAAGAATTGGAGAATAAACTAGTATT

CTTCTGGTCCCCACAGACTCAGAGAGAACCCGCCACCATGGACCAGGA

ACCCCGCCTGGAGGAAGGCCGTGTTCATCAGCCCCTACAACAGCCAGA

ACGCCGTGGCCAGCAAGATCCTGGGCCTGCCCACCCAGACCGTGGAC

AGCAGCCAGGGCAGCGAGTACGACTACGTGATCTTCACCCAGACCACC

GAGACCGCCCACAGCTGCAACGTGAACAGGTTCAACGTGGCCATCACC

AGGGCCAAGGTGGGCATCCTGTGCATCATGAGCGACAGGGACCTGTA

CGACAAGCTGCAGTTCACCAGCCTGGAGATCCCCAGGAGGAACGTGG

CCACCCTGCAGGCCGAGAACGTGACCGGCCTGTTCAAGGACTGCAGC

AAGGTGATCACCGGCCTGCACCCCACCCAGGCCCCCACCCACCTGAG

CGTGGACACCAAGTTCAAGACCGAGGGCCTGTGCGTGGACATCCCCG

GCATCCCCAAGGACATGACCTACAGGAGGCTGATCAGCATGATGGGCT

TCAAGATGAACTACCAGGTGAACGGCTACCCCAACATGTTCATCACCAG

GGAGGAGGCCATCAGGCACGTGAGGGCCTGGATCGGCTTCGACGTGG

AGGGCTGCCACGCCACCAGGGAGGCCGTGGGCACCAACCTGCCCCTG

CAGCTGGGCTTCAGCACCGGCGTGAACCTGGTGGCCGTGCCCACCGG

CTACGTGGACACCCCCAACAACACCGACTTCAGCAGGGTGAGCGCCAA

GCCCCCCCCCGGCGACCAGTTCAAGCACCTGATCCCCCTGATGTACAA

GGGCCTGCCCTGGAACGTGGTGAGGATCAAGATCGTGCAGATGCTGA

GCGACACCCTGAAGAACCTGAGCGACAGGGTGGTGTTCGTGCTGTGG

GCCCACGGCTTCGAGCTGACCAGCATGAAGTACTTCGTGAAGATCGGC

CCCGAGAGGACCTGCTGCCTGTGCGACAGGAGGGCCACCTGCTTCAG

CACCGCCAGCGACACCTACGCCTGCTGGCACCACAGCATCGGCTTCGA

CTACGTGTACAACCCCTTCATGATCGACGTGCAGCAGTGGGGCTTCAC

CGGCAACCTGCAGAGCAACCACGACCTGTACTGCCAGGTGCACGGCA

ACGCCCACGTGGCCAGCTGCGACGCCATCATGACCAGGTGCCTGGCC

GTGCACGAGTGCTTCGTGAAGAGGGTGGACTGGACCATCGAGTACCCC

ATCATCGGCGACGAGCTGAAGATCAACGCCGCCTGCAGGAAGGTGCA

GCACATGGTGGTGAAGGCCGCCCTGCTGGCCGACAAGTTCCCCGTGC

TGCACGACATCGGCAACCCCAAGGCCATCAAGTGCGTGCCCCAGGCC

GACGTGGAGTGGAAGTTCTACGACGCCCAGCCCTGCAGCGACAAGGC

CTACAAGATCGAGGAGCTGTTCTACAGCTACGCCACCCACAGCGACAA

GTTCACCGACGGCGTGTGCCTGTTCTGGAACTGCAACGTGGACAGGTA

CCCCGCCAACAGCATCGTGTGCAGGTTCGACACCAGGGTGCTGAGCAA

CCTGAACCTGCCCGGCTGCGACGGCGGCAGCCTGTACGTGAACAAGC

ACGCCTTCCACACCCCCGCCTTCGACAAGAGCGCCTTCGTGAACCTGA

AGCAGCTGCCCTTCTTCTACTACAGCGACAGCCCCTGCGAGAGCCACG

GCAAGCAGGTGGTGAGCGACATCGACTACGTGCCCCTGAAGAGCGCC

ACCTGCATCACCAGGTGCAACCTGGGCGGCGCCGTGTGCAGGCACCA

CGCCAACGAGTACAGGCTGTACCTGGACGCCTACAACATGATGATCAG

CGCCGGCTTCAGCCTGTGGGTGTACAAGCAGTTCGACACCTACAACCT

GTGGAACACCTTCACCAGGCTGCAGAGCCTGGAGAACGTGGCCTTCAA

CGTGGTGAACAAGGGCCACTTCGACGGCCAGCAGGGCGAGGTGCCCG

TGAGCATCATCAACAACACCGTGTACACCAAGGTGGACGGCGTGGACG

TGGAGCTGTTCGAGAACAAGACCACCCTGCCCGTGAACGTGGCCTTCG

AGCTGTGGGCCAAGAGGAACATCAAGCCCGTGCCCGAGGTGAAGATC

CTGAACAACCTGGGCGTGGACATCGCCGCCAACACCGTGATCTGGGAC

TACAAGAGGGACGCCCCCGCCCACATCAGCACCATCGGCGTGTGCAG

CATGACCGACATCGCCAAGAAGCCCACCGAGACCATCTGCGCCCCCCT

GACCGTGTTCTTCGACGGCAGGGTGGACGGCCAGGTGGACCTGTTCA

GGAACGCCAGGAACGGCGTGCTGATCACCGAGGGCAGCGTGAAGGGC

CTGCAGCCCAGCGTGGGCCCCAAGCAGGCCAGCCTGAACGGCGTGAC

CCTGATCGGCGAGGCCGTGAAGACCCAGTTCAACTACTACAAGAAGGT

GGACGGCGTGGTGCAGCAGCTGCCCGAGACCTACTTCACCCAGAGCA

GGAACCTGCAGGAGTTCAAGCCCAGGAGCCAGATGGAGATCGACTTCC

TGGAGCTGGCCATGGACGAGTTCATCGAGAGGTACAAGCTGGAGGGC

TACGCCTTCGAGCACATCGTGTACGGCGACTTCAGCCACAGCCAGCTG

GGCGGCCTGCACCTGCTGATCGGCCTGGCCAAGAGGTTCAAGGAGAG

CCCCTTCGAGCTGGAGGACTTCATCCCCATGGACAGCACCGTGAAGAA

CTACTTCATCACCGACGCCCAGACCGGCAGCAGCAAGTGCGTGTGCAG

CGTGATCGACCTGCTGCTGGACGACTTCGTGGAGATCATCAAGAGCCA

GGACCTGAGCGTGGTGAGCAAGGTGGTGAAGGTGACCATCGACTACA

CCGAGATCAGCTTCATGCTGTGGTGCAAGGACGGCCACGTGGAGACCT

TCTACCCCAAGCTGCAGAGCAGCCAGGCCTGGCAGCCCGGCGTGGCC

ATGCCCAACCTGTACAAGATGCAGAGGATGCTGCTGGAGAAGTGCGAC

CTGCAGAACTACGGCGACAGCGCCACCCTGCCCAAGGGCATCATGATG

AACGTGGCCAAGTACACCCAGCTGTGCCAGTACCTGAACACCCTGACC

CTGGCCGTGCCCTACAACATGAGGGTGATCCACTTCGGCGCCGGCAG

CGACAAGGGCGTGGCCCCCGGCACCGCCGTGCTGAGGCAGTGGCTG

CCCACCGGCACCCTGCTGGTGGACAGCGACCTGAACGACTTCGTGAG

CGACGCCGACAGCACCCTGATCGGCGACTGCGCCACCGTGCACACCG

CCAACAAGTGGGACCTGATCATCAGCGACATGTACGACCCCAAGACCA

AGAACGTGACCAAGGAGAACGACAGCAAGGAGGGCTTCTTCACCTACA

TCTGCGGCTTCATCCAGCAGAAGCTGGCCCTGGGCGGCAGCGTGGCC

ATCAAGATCACCGAGCACAGCTGGAACGCCGACCTGTACAAGCTGATG

GGCCACTTCGCCTGGTGGACCGCCTTCGTGACCAACGTGAACGCCAG

CAGCAGCGAGGCCTTCCTGATCGGCTGCAACTACCTGGGCAAGCCCA

GGGAGCAGATCGACGGCTACGTGATGCACGCCAACTACATCTTCTGGA

GGAACACCAACCCCATCCAGCTGAGCAGCTACAGCCTGTTCGACATGA

GCAAGTTCCCCCTGAAGCTTGATGACTCGAGCTGGTACTGCATGCACG

CAATGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCCGAGTCTCCCCCG

ACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGCCCCACTCACCAC

CTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCAATGCAGCTCAAA

ACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTT

TAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTC

AATTTCGTGCCAGCCACACCCTGGAGCTAGCAAAAAAAA

48
CTCGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCA
333

Pan-CoV
TTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAA

rAdv5-CAG-
TGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAAT

Envelope
AATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGT

CAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAG

TGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATG

GCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACT

TGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGA

GCCCCACGTTCTGCTTCACTCTCCCCATCTCCCCCCCCTCCCCACCCC

CAATTTTGTATTTATTTATTTTTTAATTATTTTGTGCAGCGATGGGGGCG

GGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGGGGCGA

GGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCA

GAGCGGCGCGCTCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGC

GGCGGCCCTATAAAAAGCGAAGCGCGCGGCGGGCGGGAGTCGCTGC

GCGCTGCCTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCGCGCCGCC

CGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGG

GACGGCCCTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGG

CTTGTTTCTTTTCTGTGGCTGCGTGAAAGCCTTGAGGGGCTCCGGGAG

GGCCCTTTGTGCGGGGGGAGCGGCTCGGGGGGTGCGTGCGTGTGTG

TGTGCGTGGGGAGCGCCGCGTGCGGCTCCGCGCTGCCCGGCGGCTG

TGAGCGCTGCGGGCGCGGCGCGGGGCTTTGTGCGCTCCGCAGTGTGC

GCGAGGGGAGCGCGGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGG

GCTGCGAGGGGAACAAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGG

GTGAGCAGGGGGTGTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTG

CACCCCCCTCCCCGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGG

GCTCCGTACGGGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGG

TGGCGGCAGGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCC

GGGGAGGGCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGC

GGCTGTCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCG

TGCGAGAGGGCGCAGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCG

AAATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGAA

GCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTCGT

GCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGGCTG

TCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGGCGGG

GTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGCTAACCA

TGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGTGCTGGTT

ATTGTGCTGTCTCATCATTTTGGCAAAGAATTGGAGAATAAACTAGTATT

CTTCTGGTCCCCACAGACTCAGAGAGAACCCGCCACCATGTACAGCTT

CGTGAGCGAGGAGACCGGCACCCTGATCGTGAACAGCGTGCTGCTGT

TCCTGGCCTTCGTGGTGTTCCTGCTGGTGACCCTGGCCATCCTGACCG

CCCTGAGGCTGTGCGCCTACTGCTGCAACATCGTGAACGTGAGCCTGG

TGAAGCCCAGCTTCTACGTGTACAGCAGGGTGAAGAACCTGAACAGCA

GCAGGGTGCCCGACCTGCTGGTGTGATGACTCGAGCTGGTACTGCATG

CACGCAATGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCCGAGTCTCC

CCCGACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGCCCCACTCA

CCACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCAATGCAGCT

CAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAA

CCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTT

GGTCAATTTCGTGCCAGCCACACCCTGGAGCTAGCAAAAAAAA

49
CTCGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCA
334

Pan-CoV
TTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAA

rAdv5-CAG-
TGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAAT

Membrane
AATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGT

CAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAG

TGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATG

GCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACT

TGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGA

GCCCCACGTTCTGCTTCACTCTCCCCATCTCCCCCCCCTCCCCACCCC

CAATTTTGTATTTATTTATTTTTTAATTATTTTGTGCAGCGATGGGGGCG

GGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGGGGCGA

GGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCA

GAGCGGCGCGCTCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGC

GGCGGCCCTATAAAAAGCGAAGCGCGCGGCGGGCGGGAGTCGCTGC

GCGCTGCCTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCGCGCCGCC

CGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGG

GACGGCCCTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGG

CTTGTTTCTTTTCTGTGGCTGCGTGAAAGCCTTGAGGGGCTCCGGGAG

GGCCCTTTGTGCGGGGGGAGCGGCTCGGGGGGTGCGTGCGTGTGTG

TGTGCGTGGGGAGCGCCGCGTGCGGCTCCGCGCTGCCCGGCGGCTG

TGAGCGCTGCGGGCGCGGCGCGGGGCTTTGTGCGCTCCGCAGTGTGC

GCGAGGGGAGCGCGGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGG

GCTGCGAGGGGAACAAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGG

GTGAGCAGGGGGTGTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTG

CACCCCCCTCCCCGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGG

GCTCCGTACGGGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGG

TGGCGGCAGGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCC

GGGGAGGGCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGC

GGCTGTCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCG

TGCGAGAGGGCGCAGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCG

AAATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGAA

GCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTCGT

GCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGGCTG

TCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGGCGGG

GTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGCTAACCA

TGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGTGCTGGTT

ATTGTGCTGTCTCATCATTTTGGCAAAGAATTGGAGAATAAACTAGTATT

CTTCTGGTCCCCACAGACTCAGAGAGAACCCGCCACCATGGCCGACAG

CAACGGCACCATCACCGTGGAGGAGCTGAAGAAGCTGCTGGAGCAGT

GGAACCTGGTGATCGGCTTCCTGTTCCTGACCTGGATCTGCCTGCTGC

AGTTCGCCTACGCCAACAGGAACAGGTTCCTGTACATCATCAAGCTGAT

CTTCCTGTGGCTGCTGTGGCCCGTGACCCTGGCCTGCTTCGTGCTGGC

CGCCGTGTACAGGATCAACTGGATCACCGGCGGCATCGCCATCGCCAT

GGCCTGCCTGGTGGGCCTGATGTGGCTGAGCTACTTCATCGCCAGCTT

CAGGCTGTTCGCCAGGACCAGGAGCATGTGGAGCTTCAACCCCGAGA

CCAACATCCTGCTGAACGTGCCCCTGCACGGCACCATCCTGACCAGGC

CCCTGCTGGAGAGCGAGCTGGTGATCGGCGCCGTGATCCTGAGGGGC

CACCTGAGGATCGCCGGCCACCACCTGGGCAGGTGCGACATCAAGGA

CCTGCCCAAGGAGATCACCGTGGCCACCAGCAGGACCCTGAGCTACTA

CAAGCTGGGCGCCAGCCAGAGGGTGGCCGGCGACAGCGGCTTCGCC

GCCTACAGCAGGTACAGGATCGGCAACTACAAGCTGAACACCGACCAC

AGCAGCAGCAGCGACAACATCGCCCTGCTGGTGCAGTGATGACTCGAG

CTGGTACTGCATGCACGCAATGCTAGCTGCCCCTTTCCCGTCCTGGGT

ACCCCGAGTCTCCCCCGACCTCGGGTCCCAGGTATGCTCCCACCTCCA

CCTGCCCCACTCACCACCTCTGCTAGTTCCAGACACCTCCCAAGCACG

CAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAA

CAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACT

AACCCCAGGGTTGGTCAATTTCGTGCCAGCCACACCCTGGAGCTAGCA

AAAAAAA

50
CTCGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCA
335

Pan-CoV
TTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAA

rAdv5-CAG-
TGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAAT

ORF7a
AATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGT

CAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAG

TGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATG

GCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACT

TGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGA

GCCCCACGTTCTGCTTCACTCTCCCCATCTCCCCCCCCTCCCCACCCC

CAATTTTGTATTTATTTATTTTTTAATTATTTTGTGCAGCGATGGGGGCG

GGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGGGGCGA

GGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCA

GAGCGGCGCGCTCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGC

GGCGGCCCTATAAAAAGCGAAGCGCGCGGCGGGCGGGAGTCGCTGC

GCGCTGCCTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCGCGCCGCC

CGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGG

GACGGCCCTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGG

CTTGTTTCTTTTCTGTGGCTGCGTGAAAGCCTTGAGGGGCTCCGGGAG

GGCCCTTTGTGCGGGGGGAGCGGCTCGGGGGGTGCGTGCGTGTGTG

TGTGCGTGGGGAGCGCCGCGTGCGGCTCCGCGCTGCCCGGCGGCTG

TGAGCGCTGCGGGCGCGGCGCGGGGCTTTGTGCGCTCCGCAGTGTGC

GCGAGGGGAGCGCGGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGG

GCTGCGAGGGGAACAAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGG

GTGAGCAGGGGGTGTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTG

CACCCCCCTCCCCGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGG

GCTCCGTACGGGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGG

TGGCGGCAGGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCC

TGGCGGCAGGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCC

GGGGAGGGCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGC

GGCTGTCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCG

TGCGAGAGGGCGCAGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCG

AAATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGAA

GCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTCGT

GCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGGCTG

TCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGGCGGG

GTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGCTAACCA

TGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGTGCTGGTT

ATTGTGCTGTCTCATCATTTTGGCAAAGAATTGGAGAATAAACTAGTATT

CTTCTGGTCCCCACAGACTCAGAGAGAACCCGCCACCATGAAGATCAT

CCTGTTCCTGGCCCTGATCACCCTGGCCACCTGCGAGCTGTACCACTA

CCAGGAGTGCGTGAGGGGCACCACCGTGTGATGACTCGAGCTGGTAC

TGCATGCACGCAATGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCCGA

GTCTCCCCCGACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGCCC

CACTCACCACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCAAT

GCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGT

GATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCA

GGGTTGGTCAATTTCGTGCCAGCCACACCCTGGAGCTAGCAAAAAAAA

51
CTCGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCA
336

Pan-CoV
TTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAA

rAdv-CAG-
TGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAAT

NSP3
AATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGT

CAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAG

TGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATG

GCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACT

TGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGA

GCCCCACGTTCTGCTTCACTCTCCCCATCTCCCCCCCCTCCCCACCCC

CAATTTTGTATTTATTTATTTTTTAATTATTTTGTGCAGCGATGGGGGCG

GGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGGGGCGA

GGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCA

GAGCGGCGCGCTCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGC

GGCGGCCCTATAAAAAGCGAAGCGCGCGGCGGGCGGGAGTCGCTGC

GCGCTGCCTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCGCGCCGCC

CGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGG

GACGGCCCTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGG

CTTGTTTCTTTTCTGTGGCTGCGTGAAAGCCTTGAGGGGCTCCGGGAG

GGCCCTTTGTGCGGGGGGAGCGGCTCGGGGGGTGCGTGCGTGTGTG

TGTGCGTGGGGAGCGCCGCGTGCGGCTCCGCGCTGCCCGGCGGCTG

TGAGCGCTGCGGGCGCGGCGCGGGGCTTTGTGCGCTCCGCAGTGTGC

GCGAGGGGAGCGCGGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGG

GCTGCGAGGGGAACAAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGG

GTGAGCAGGGGGTGTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTG

CACCCCCCTCCCCGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGG

GCTCCGTACGGGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGG

TGGCGGCAGGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCC

GGGGAGGGCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGC

GGCTGTCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCG

TGCGAGAGGGCGCAGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCG

AAATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGAA

GCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTCGT

GCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGGCTG

TCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGGCGGG

GTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGCTAACCA

TGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGTGCTGGTT

ATTGTGCTGTCTCATCATTTTGGCAAAGAATTGGAGAATAAACTAGTATT

CTTCTGGTCCCCACAGACTCAGAGAGAACCCGCCACCATGGCCCCCAC

CAAGGTGACCTTCGGCGACGACACCGTGATCGAGGTGCAGGGCTACA

AGAGCGTGAACATCACCTTCGAGCTGGACGAGAGGATCGACAAGGTGC

TGAACGAGAAGTGCAGCGCCTACACCGTGGAGCTGGGCACCGAGGTG

AACGAGTTCGCCTGCGTGGTGGCCGACGCCGTGATCAAGACCCTGCA

GCCCGTGAGCGAGCTGCTGACCCCCCTGGGCATCGACCTGGACGAGT

GGAGCATGGCCACCTACTACCTGTTCGACGAGAGCGGCGAGTTCAAGC

TGGCCAGCCACATGTACTGCAGCTTCTACCCCCCCGACGAGGACGAGG

AGGAGGGCGACTGCGAGGAGGAGGAGTTCGAGCCCAGCACCCAGTAC

GAGTACGGCACCGAGGACGACTACCAGGGCAAGCCCCTGGAGTTCGG

CGCCACCAGCGCCGCCCTGCAGCCCGAGGAGGAGCAGGAGGAGGAC

TGGCTGGACGACGACAGCCAGCAGACCGTGGGCCAGCAGGACGGCAG

CGAGGACAACCAGACCACCACCATCCAGACCATCGTGGAGGTGCAGC

CCCAGCTGGAGATGGAGCTGACCCCCGTGGTGCAGACCATCGAGGTG

AACAGCTTCAGCGGCTACCTGAAGCTGACCGACAACGTGTACATCAAG

AACGCCGACATCGTGGAGGAGGCCAAGAAGGTGAAGCCCACCGTGGT

GGTGAACGCCGCCAACGTGTACCTGAAGCACGGCGGCGGCGTGGCCG

GCGCCCTGAACAAGGCCACCAACAACGCCATGCAGGTGGAGAGCGAC

GACTACATCGCCACCAACGGCCCCCTGAAGGTGGGCGGCAGCTGCGT

GCTGAGCGGCCACAACCTGGCCAAGCACTGCCTGCACGTGGTGGGCC

CCAACGTGAACAAGGGCGAGGACATCCAGCTGCTGAAGAGCGCCTAC

GAGAACTTCAACCAGCACGAGGTGCTGCTGGCCCCCCTGCTGAGCGC

CGGCATCTTCGGCGCCGACCCCATCCACAGCCTGAGGGTGTGCGTGG

ACACCGTGAGGACCAACGTGTACCTGGCCGTGTTCGACAAGAACCTGT

ACGACAAGCTGGTGAGCAGCTTCCTGGAGATGAAGAGCGAGAAGCAG

GTGGAGCAGAAGATCGCCGAGATCCCCAAGGAGGAGGTGAAGCCCTT

CATCACCGAGAGCAAGCCCAGCGTGGAGCAGAGGAAGCAGGACGACA

AGAAGATCAAGGCCTGCGTGGAGGAGGTGACCACCACCCTGGAGGAG

ACCAAGTTCCTGACCGAGAACCTGCTGCTGTACATCGACATCAACGGC

AACCTGCACCCCGACAGCGCCACCCTGGTGAGCGACATCGACATCACC

TTCCTGAAGAAGGACGCCCCCTACATCGTGGGCGACGTGGTGCAGGA

GGGCGTGCTGACCGCCGTGGTGATCCCCACCAAGAAGGCCGGCGGCA

CCACCGAGATGCTGGCCAAGGCCCTGAGGAAGGTGCCCACCGACAAC

TACATCACCACCTACCCCGGCCAGGGCCTGAACGGCTACACCGTGGAG

GAGGCCAAGACCGTGCTGAAGAAGTGCAAGAGCGCCTTCTACATCCTG

CCCAGCATCATCAGCAACGAGAAGCAGGAGATCCTGGGCACCGTGAG

CTGGAACCTGAGGGAGATGCTGGCCCACGCCGAGGAGACCAGGAAGC

TGATGCCCGTGTGCGTGGAGACCAAGGCCATCGTGAGCACCATCCAGA

GGAAGTACAAGGGCATCAAGATCCAGGAGGGCGTGGTGGACTACGGC

GCCAGGTTCTACTTCTACACCAGCAAGACCACCGTGGCCAGCCTGATC

AACACCCTGAACGACCTGAACGAGACCCTGGTGACCATGCCCCTGGGC

TACGTGACCCACGGCCTGAACCTGGAGGAGGCCGCCAGGTACATGAG

GAGCCTGAAGGTGCCCGCCACCGTGAGCGTGAGCAGCCCCGACGCCG

TGACCGCCTACAACGGCTACCTGACCAGCAGCAGCAAGACCCCCGAG

GAGCACTTCATCGAGACCATCAGCCTGGCCGGCAGCTACAAGGACTGG

AGCTACAGCGGCCAGAGCACCCAGCTGGGCATCGAGTTCCTGAAGAG

GGGCGACAAGAGCGTGTACTACACCAGCAACCCCACCACCTTCCACCT

GGACGGCGAGGTGATCACCTTCGACAACCTGAAGACCCTGCTGAGCCT

GAGGGAGGTGAGGACCATCAAGGTGTTCACCACCGTGGACAACATCAA

CCTGCACTGATGACTCGAGCTGGTACTGCATGCACGCAATGCTAGCTG

CCCCTTTCCCGTCCTGGGTACCCCGAGTCTCCCCCGACCTCGGGTCCC

AGGTATGCTCCCACCTCCACCTGCCCCACTCACCACCTCTGCTAGTTCC

AGACACCTCCCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAG

CCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAA

AGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGC

CACACCCTGGAGCTAGCAAAAAAAA

52
CTCGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCA
337

Pan-CoV
TTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAA

rAdv5-CAG-
TGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAAT

NSP5
AATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGT

CAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAG

TGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATG

GCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACT

TGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGA

GCCCCACGTTCTGCTTCACTCTCCCCATCTCCCCCCCCTCCCCACCCC

CAATTTTGTATTTATTTATTTTTTAATTATTTTGTGCAGCGATGGGGGCG

GGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGGGGCGA

GGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCA

GAGCGGCGCGCTCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGC

GGCGGCCCTATAAAAAGCGAAGCGCGCGGCGGGCGGGAGTCGCTGC

GCGCTGCCTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCGCGCCGCC

CGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGG

GACGGCCCTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGG

CTTGTTTCTTTTCTGTGGCTGCGTGAAAGCCTTGAGGGGCTCCGGGAG

GGCCCTTTGTGCGGGGGGAGCGGCTCGGGGGGTGCGTGCGTGTGTG

TGTGCGTGGGGAGCGCCGCGTGCGGCTCCGCGCTGCCCGGCGGCTG

TGAGCGCTGCGGGCGCGGCGCGGGGCTTTGTGCGCTCCGCAGTGTGC

GCGAGGGGAGCGCGGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGG

GCTGCGAGGGGAACAAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGG

GTGAGCAGGGGGTGTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTG

CACCCCCCTCCCCGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGG

GCTCCGTACGGGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGG

TGGCGGCAGGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCC

GGGGAGGGCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGC

GGCTGTCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCG

TGCGAGAGGGCGCAGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCG

AAATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGAA

GCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTCGT

GCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGGCTG

TCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGGCGGG

GTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGCTAACCA

TGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGTGCTGGTT

ATTGTGCTGTCTCATCATTTTGGCAAAGAATTGGAGAATAAACTAGTATT

CTTCTGGTCCCCACAGACTCAGAGAGAACCCGCCACCATGAGCGGCTT

CAGGAAGATGGCCTTCCCCAGCGGCAAGGTGGAGGGCTGCATGGTGC

AGGTGACCTGCGGCACCACCACCCTGAACGGCCTGTGGCTGGACGAC

GTGGTGTACTGCCCCAGGCACGTGATCTGCACCAGCGAGGACATGCTG

AACCCCAACTACGAGGACCTGCTGATCAGGAAGAGCAACCACAACTTC

CTGGTGCAGGCCGGCAACGTGCAGCTGAGGGTGATCGGCCACAGCAT

GCAGAACTGCGTGCTGAAGCTGAAGGTGGACACCGCCAACCCCAAGA

CCCCCAAGTACAAGTTCGTGAGGATCCAGCCCGGCCAGACCTTCAGCG

TGCTGGCCTGCTACAACGGCAGCCCCAGCGGCGTGTACCAGTGCGCC

ATGAGGCCCAACTTCACCATCAAGGGCAGCTTCCTGAACGGCAGCTGC

GGCAGCGTGGGCTTCAACATCGACTACGACTGCGTGAGCTTCTGCTAC

ATGCACCACATGGAGCTGCCCACCGGCGTGCACGCCGGCACCGACCT

GGAGGGCAACTTCTACGGCCCCTTCGTGGACAGGCAGACCGCCCAGG

CCGCCGGCACCGACACCACCATCACCGTGAACGTGCTGGCCTGGCTG

TACGCCGCCGTGATCAACGGCGACAGGTGGTTCCTGAACAGGTTCACC

ACCACCCTGAACGACTTCAACCTGGTGGCCATGAAGTACAACTACGAG

CCCCTGACCCAGGACCACGTGGACATCCTGGGCCCCCTGAGCGCCCA

GACCGGCATCGCCGTGCTGGACATGTGCGCCAGCCTGAAGGAGCTGC

TGCAGAACGGCATGAACGGCAGGACCATCCTGGGCAGCGCCCTGCTG

GAGGACGAGTTCACCCCCTTCGACGTGGTGAGGCAGTGCAGCGGCGT

GACCTTCCAGTGATGACTCGAGCTGGTACTGCATGCACGCAATGCTAG

CTGCCCCTTTCCCGTCCTGGGTACCCCGAGTCTCCCCCGACCTCGGGT

CCCAGGTATGCTCCCACCTCCACCTGCCCCACTCACCACCTCTGCTAG

TTCCAGACACCTCCCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGC

CTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAA

CGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGC

CAGCCACACCCTGGAGCTAGCAAAAAAAA

53
CTCGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCA
338

Pan-CoV
TTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAA

rAdv5-CAG-
TGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAAT

NSP12
AATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGT

CAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAG

TGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATG

GCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACT

TGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGA

GCCCCACGTTCTGCTTCACTCTCCCCATCTCCCCCCCCTCCCCACCCC

CAATTTGTATTTTATTTATTTTTTAATTATTTTGTGCAGCGATGGGGGCG

GGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGGGGCGA

GGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCA

GAGCGGCGCGCTCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGC

GGCGGCCCTATAAAAAGCGAAGCGCGCGGCGGGCGGGAGTCGCTGC

GCGCTGCCTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCGCGCCGCC

CGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGG

GACGGCCCTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGG

CTTGTTTCTTTTCTGTGGCTGCGTGAAAGCCTTGAGGGGCTCCGGGAG

GGCCCTTTGTGCGGGGGGAGCGGCTCGGGGGGTGCGTGCGTGTGTG

TGTGCGTGGGGAGCGCCGCGTGCGGCTCCGCGCTGCCCGGCGGCTG

TGAGCGCTGCGGGCGCGGCGCGGGGCTTTGTGCGCTCCGCAGTGTGC

GCGAGGGGAGCGCGGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGG

GCTGCGAGGGGAACAAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGG

GTGAGCAGGGGGTGTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTG

CACCCCCCTCCCCGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGG

GCTCCGTACGGGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGG

TGGCGGCAGGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCC

GGGGAGGGCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGC

GGCTGTCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCG

TGCGAGAGGGCGCAGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCG

AAATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGAA

GCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTCGT

GCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGGCTG

TCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGGCGGG

GTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGCTAACCA

TGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGTGCTGGTT

ATTGTGCTGTCTCATCATTTTGGCAAAGAATTGGAGAATAAACTAGTATT

CTTCTGGTCCCCACAGACTCAGAGAGAACCCGCCACCATGAGCGCCGA

CGCCCAGAGCTTCCTGAACAGGGTGTGCGGCGTGAGCGCCGCCAGGC

TGACCCCCTGCGGCACCGGCACCAGCACCGACGTGGTGTACAGGGCC

TTCGACATCTACAACGACAAGGTGGCCGGCTTCGCCAAGTTCCTGAAG

ACCAACTGCTGCAGGTTCCAGGAGAAGGACGAGGACGACAACCTGATC

GACAGCTACTTCGTGGTGAAGAGGCACACCTTCAGCAACTACCAGCAC

GAGGAGACCATCTACAACCTGCTGAAGGACTGCCCCGCCGTGGCCAA

GCACGACTTCTTCAAGTTCAGGATCGACGGCGACATGGTGCCCCACAT

CAGCAGGCAGAGGCTGACCAAGTACACCATGGCCGACCTGGTGTACG

CCCTGAGGCACTTCGACGAGGGCAACTGCGACACCCTGAAGGAGATC

CTGGTGACCTACAACTGCTGCGACGACGACTACTTCAACAAGAAGGAC

TGGTACGACTTCGTGGAGAACCCCGACATCCTGAGGGTGTACGCCAAC

CTGGGCGAGAGGGTGAGGCAGGCCCTGCTGAAGACCGTGCAGTTCTG

CGACGCCATGAGGAACGCCGGCATCGTGGGCGTGCTGACCCTGGACA

ACCAGGACCTGAACGGCAACTGGTACGACTTCGGCGACTTCATCCAGA

CCACCCCCGGCAGCGGCGTGCCCGTGGTGGACAGCTACTACAGCCTG

CTGATGCCCATCCTGACCCTGACCAGGGCCCTGACCGCCGAGAGCCA

CGTGGACACCGACCTGACCAAGCCCTACATCAAGTGGGACCTGCTGAA

GTACGACTTCACCGAGGAGAGGCTGAAGCTGTTCGACAGGTACTTCAA

GTACTGGGACCAGACCTACCACCCCAACTGCGTGAACTGCCTGGACGA

CAGGTGCATCCTGCACTGCGCCAACTTCAACGTGCTGTTCAGCACCGT

GTTCCCCCCCACCAGCTTCGGCCCCCTGGTGAGGAAGATCTTCGTGGA

CGGCGTGCCCTTCGTGGTGAGCACCGGCTACCACTTCAGGGAGCTGG

GCGTGGTGCACAACCAGGACGTGAACCTGCACAGCAGCAGGCTGAGC

TTCAAGGAGCTGCTGGTGTACGCCGCCGACCCCGCCATGCACGCCGC

CAGCGGCAACCTGCTGCTGGACAAGAGGACCACCTGCTTCAGCGTGG

CCGCCCTGACCAACAACGTGGCCTTCCAGACCGTGAAGCCCGGCAACT

TCAACAAGGACTTCTACGACTTCGCCGTGAGCAAGGGCTTCTTCAAGG

AGGGCAGCAGCGTGGAGCTGAAGCACTTCTTCTTCGCCCAGGACGGC

AACGCCGCCATCAGCGACTACGACTACTACAGGTACAACCTGCCCACC

ATGTGCGACATCAGGCAGCTGCTGTTCGTGGTGGAGGTGGTGGACAAG

TACTTCGACTGCTACGACGGCGGCTGCATCAACGCCAACCAGGTGATC

GTGAACAACCTGGACAAGAGCGCCGGCTTCCCCTTCAACAAGTGGGGC

AAGGCCAGGCTGTACTACGACAGCATGAGCTACGAGGACCAGGACGC

CCTGTTCGCCTACACCAAGAGGAACGTGATCCCCACCATCACCCAGAT

GAACCTGAAGTACGCCATCAGCGCCAAGAACAGGGCCAGGACCGTGG

CCGGCGTGAGCATCTGCAGCACCATGACCAACAGGCAGTTCCACCAGA

AGCTGCTGAAGAGCATCGCCGCCACCAGGGGCGCCACCGTGGTGATC

GGCACCAGCAAGTTCTACGGCGGCTGGCACAACATGCTGAAGACCGTG

TACAGCGACGTGGAGAACCCCCACCTGATGGGCTGGGACTACCCCAA

GTGCGACAGGGCCATGCCCAACATGCTGAGGATCATGGCCAGCCTGG

TGCTGGCCAGGAAGCACACCACCTGCTGCAGCCTGAGCCACAGGTTCT

ACAGGCTGGCCAACGAGTGCGCCCAGGTGCTGAGCGAGATGGTGATG

TGCGGCGGCAGCCTGTACGTGAAGCCCGGCGGCACCAGCAGCGGCG

ACGCCACCACCGCCTACGCCAACAGCGTGTTCAACATCTGCCAGGCCG

TGACCGCCAACGTGAACGCCCTGCTGAGCACCGACGGCAACAAGATC

GCCGACAAGTACGTGAGGAACCTGCAGCACAGGCTGTACGAGTGCCT

GTACAGGAACAGGGACGTGGACACCGACTTCGTGAACGAGTTCTACGC

CTACCTGAGGAAGCACTTCAGCATGATGATCCTGAGCGACGACGCCGT

GGTGTGCTTCAACAGCACCTACGCCAGCCAGGGCCTGGTGGCCAGCA

TCAAGAACTTCAAGAGCGTGCTGTACTACCAGAACAACGTGTTCATGAG

CGAGGCCAAGTGCTGGACCGAGACCGACCTGACCAAGGGCCCCCACG

AGTTCTGCAGCCAGCACACCATGCTGGTGAAGCAGGGCGACGACTACG

TGTACCTGCCCTACCCCGACCCCAGCAGGATCCTGGGCGCCGGCTGC

TTCGTGGACGACATCGTGAAGACCGACGGCACCCTGATGATCGAGAGG

TTCGTGAGCCTGGCCATCGACGCCTACCCCCTGACCAAGCACCCCAAC

CAGGAGTACGCCGACGTGTTCCACCTGTACCTGCAGTACATCAGGAAG

CTGCACGACGAGCTGACCGGCCACATGCTGGACATGTACAGCGTGATG

CTGACCAACGACAACACCAGCAGGTACTGGGAGCCCGAGTTCTACGAG

GCCATGTACACCCCCCACACCGTGCTGCAGTGATGACTCGAGCTGGTA

CTGCATGCACGCAATGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCCG

AGTCTCCCCCGACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGCC

CCACTCACCACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCAA

TGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAG

TGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCC

AGGGTTGGTCAATTTCGTGCCAGCCACACCCTGGAGCTAGCAAAAAAA

A

54
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGTG
339

Pan-CoV (B1)
AACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAGCTTC

ACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCT

GCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTG

GTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGA

CAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAG

CAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGAT

CAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTA

CTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTA

CAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCT

GATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGT

TCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACAC

CCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCCTGG

AGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGA

CCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGC

AGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCA

GCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGA

CGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGCACCC

TGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCA

GGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACC

TGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGT

ACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGC

GTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTG

AGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGAC

AGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCA

GACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCAC

CGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGG

GCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAGC

ACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTGCAG

AGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCTACAG

GGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGT

GCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACT

TCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACA

AGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCA

CCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACC

CCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACAC

CAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGG

TGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTG

TACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGAT

CGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCG

GCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCGGC

AGCGCCAGCAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAG

CCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCA

TCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGA

GCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACA

GCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCACCC

AGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACAAGAAC

ACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCCCCC

ATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCGACCCC

AGCAAGCCCAGCAAGAGGAGCCCCATCGAGGACCTGCTGTTCAACAAG

GTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCT

GGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACG

GCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAG

TACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTT

CGGCGCCGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATGGCCT

ACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACC

AGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGG

ACAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGTG

GTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAG

CAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAG

GCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCG

GCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGG

GCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAG

CGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGG

GCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTG

TTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACC

GCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGG

CGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTT

CTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAA

CTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCT

GCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCA

ACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAG

GTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGG

CAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTT

CATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTG

CATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGGCA

GCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGC

GTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCTGTTA

AAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTATGAGCGACAAC

GGCCCCCAGAACCAGAGGAACGCCCCCAGGATCACCTTCGGCGGCCC

CAGCGACAGCACCGGCAGCAACCAGAACGGCGAGAGGAGCGGCGCC

AGGAGCAAGCAGAGGAGGCCCCAGGGCCTGCCCAACAACACCGCCAG

CTGGTTCACCGCCCTGACCCAGCACGGCAAGGAGGACCTGAAGTTCCC

CAGGGGCCAGGGCGTGCCCATCAACACCAACAGCAGCCCCGACGACC

AGATCGGCTACTACAGGAGGGCCACCAGGAGGATCAGGGGCGGCGAC

GGCAAGATGAAGGACCTGAGCCCCAGGTGGTACTTCTACTACCTGGGC

ACCGGCCCCGAGGCCGGCCTGCCCTACGGCGCCAACAAGGACGGCAT

CATCTGGGTGGCCACCGAGGGCGCCCTGAACACCCCCAAGGACCACA

TCGGCACCAGGAACCCCGCCAACAACGCCGCCATCGTGCTGCAGCTG

CCCCAGGGCACCACCCTGCCCAAGGGCTTCTACGCCGAGGGCAGCAG

GGGCGGCAGCCAGGCCAGCAGCAGGAGCAGCAGCAGGAGCAGGAAC

AGCAGCAGGAACAGCACCCCCGGCAGCAGCAGGGGCACCAGCCCCG

CCAGGATGGCCGGCAACGGCGGCGACGCCGCCCTGGCCCTGCTGCT

GCTGGACAGGCTGAACCAGCTGGAGAGCAAGATGAGCGGCAAGGGCC

AGCAGCAGCAGGGCCAGACCGTGACCAAGAAGAGCGCCGCCGAGGCC

AGCAAGAAGCCCAGGCAGAAGAGGACCGCCACCAAGGCCTACAACGT

GACCCAGGCCTTCGGCAGGAGGGGCCCCGAGCAGACCCAGGGCAACT

TCGGCGACCAGGAGCTGATCAGGCAGGGCACCGACTACAAGCACTGG

CCCCAGATCGCCCAGTTCGCCCCCAGCGCCAGCGCCTTCTTCGGCATG

AGCAGGATCGGCATGGAGGTGACCCCCAGCGGCACCTGGCTGACCTA

CACCGGCGCCATCAAGCTGGACGACAAGGACCCCAACTTCAAGGACCA

GGTGATCCTGCTGAACAAGCACATCGACGCCTACAAGACCTTCCCCCC

CACCGAGCCCAAGAAGGACAAGAAGAAGAAGGCCGACGAGACCCAGG

CCCTGCCCCAGAGGCAGAAGAAGCAGCAGACCGTGACCCTGCTGCCC

GCCGCCGACCTGGACGACTTCAGCAAGCAGCTGCAGCAGAGCATGAG

CAGCGCCGACAGCACCCAGGCCTGATGA

55
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGTG
348

Pan-CoV (B2)
AACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAGCTTC

ACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCT

GCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTG

GTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGA

CAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAG

CAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGAT

CAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTA

CTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTA

CAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCT

GATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGT

TCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACAC

CCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCCTGG

AGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGA

CCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGC

AGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCA

GCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGA

CGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGCACCC

TGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCA

GGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACC

TGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGT

ACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGC

GTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTG

AGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGAC

AGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCA

GACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCAC

CGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGG

GCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAGC

ACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTGCAG

AGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCTACAG

GGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGT

GCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACT

TCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACA

AGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCA

CCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACC

CCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACAC

CAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGG

TGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTG

TACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGAT

CGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCG

GCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCGGC

AGCGCCAGCAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAG

CCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCA

TCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGA

GCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACA

GCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCACCC

AGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACAAGAAC

ACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCCCCC

ATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCGACCCC

AGCAAGCCCAGCAAGAGGAGCCCCATCGAGGACCTGCTGTTCAACAAG

GTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCT

GGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACG

GCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAG

TACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTT

CGGCGCCGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATGGCCT

ACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACC

AGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGG

ACAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGTG

GTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAG

CAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAG

GCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCG

GCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGG

GCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAG

CGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGG

GCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTG

TTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACC

GCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGG

CGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTT

CTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAA

CTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCT

GCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCA

ACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAG

GTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGG

CAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTT

CATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTG

CATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGGCA

GCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGC

GTGAAGCTGCACTACACCTGATGA

56
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGTG
341

Pan-CoV (B4)
AACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAGCTTC

ACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCT

GCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTG

GTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGA

CAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAG

CAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGAT

CAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTA

CTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTA

CAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCT

GATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGT

TCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACAC

CCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCCTGG

AGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGA

CCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGC

AGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCA

GCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGA

CGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGCACCC

TGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCA

GGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACC

TGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGT

ACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGC

GTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTG

AGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGAC

AGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCA

GACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCAC

CGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGG

GCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAGC

ACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTGCAG

AGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCTACAG

GGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGT

GCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACT

TCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACA

AGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCA

CCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACC

CCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACAC

CAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGG

TGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTG

TACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGAT

CGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCG

GCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCGGC

AGCGCCAGCAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAG

CCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCA

TCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGA

GCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACA

GCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCACCC

AGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACAAGAAC

ACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCCCCC

ATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCGACCCC

AGCAAGCCCAGCAAGAGGAGCCCCATCGAGGACCTGCTGTTCAACAAG

GTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCT

GGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACG

GCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAG

TACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTT

CGGCGCCGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATGGCCT

ACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACC

AGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGG

ACAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGTG

GTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAG

CAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAG

GCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCG

GCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGG

GCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAG

CGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGG

GCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTG

TTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACC

GCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGG

CGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTT

CTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAA

CTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCT

GCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCA

ACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAG

GTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGG

CAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTT

CATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTG

CATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGGCA

GCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGC

GTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCTGTTA

AAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTGACCAGGAACCC

CGCCTGGAGGAAGGCCGTGTTCATCAGCCCCTACAACAGCCAGAACGC

CGTGGCCAGCAAGATCCTGGGCCTGCCCACCCAGACCGTGGACAGCA

GCCAGGGCAGCGAGTACGACTACGTGATCTTCACCCAGACCACCGAGA

CCGCCCACAGCTGCAACGTGAACAGGTTCAACGTGGCCATCACCAGGG

CCAAGGTGGGCATCCTGTGCATCATGAGCGACAGGGACCTGTACGACA

AGCTGCAGTTCACCAGCCTGGAGATCCCCAGGAGGAACGTGGCCACC

CTGCAGGCCGAGAACGTGACCGGCCTGTTCAAGGACTGCAGCAAGGT

GATCACCGGCCTGCACCCCACCCAGGCCCCCACCCACCTGAGCGTGG

ACACCAAGTTCAAGACCGAGGGCCTGTGCGTGGACATCCCCGGCATCC

CCAAGGACATGACCTACAGGAGGCTGATCAGCATGATGGGCTTCAAGA

TGAACTACCAGGTGAACGGCTACCCCAACATGTTCATCACCAGGGAGG

AGGCCATCAGGCACGTGAGGGCCTGGATCGGCTTCGACGTGGAGGGC

TGCCACGCCACCAGGGAGGCCGTGGGCACCAACCTGCCCCTGCAGCT

GGGCTTCAGCACCGGCGTGAACCTGGTGGCCGTGCCCACCGGCTACG

TGGACACCCCCAACAACACCGACTTCAGCAGGGTGAGCGCCAAGCCC

CCCCCCGGCGACCAGTTCAAGCACCTGATCCCCCTGATGTACAAGGGC

CTGCCCTGGAACGTGGTGAGGATCAAGATCGTGCAGATGCTGAGCGAC

ACCCTGAAGAACCTGAGCGACAGGGTGGTGTTCGTGCTGTGGGCCCA

CGGCTTCGAGCTGACCAGCATGAAGTACTTCGTGAAGATCGGCCCCGA

GAGGACCTGCTGCCTGTGCGACAGGAGGGCCACCTGCTTCAGCACCG

CCAGCGACACCTACGCCTGCTGGCACCACAGCATCGGCTTCGACTACG

TGTACAACCCCTTCATGATCGACGTGCAGCAGTGGGGCTTCACCGGCA

ACCTGCAGAGCAACCACGACCTGTACTGCCAGGTGCACGGCAACGCC

CACGTGGCCAGCTGCGACGCCATCATGACCAGGTGCCTGGCCGTGCA

CGAGTGCTTCGTGAAGAGGGTGGACTGGACCATCGAGTACCCCATCAT

CGGCGACGAGCTGAAGATCAACGCCGCCTGCAGGAAGGTGCAGCACA

TGGTGGTGAAGGCCGCCCTGCTGGCCGACAAGTTCCCCGTGCTGCAC

GACATCGGCAACCCCAAGGCCATCAAGTGCGTGCCCCAGGCCGACGT

GGAGTGGAAGTTCTACGACGCCCAGCCCTGCAGCGACAAGGCCTACAA

GATCGAGGAGCTGTTCTACAGCTACGCCACCCACAGCGACAAGTTCAC

CGACGGCGTGTGCCTGTTCTGGAACTGCAACGTGGACAGGTACCCCG

CCAACAGCATCGTGTGCAGGTTCGACACCAGGGTGCTGAGCAACCTGA

ACCTGCCCGGCTGCGACGGCGGCAGCCTGTACGTGAACAAGCACGCC

TTCCACACCCCCGCCTTCGACAAGAGCGCCTTCGTGAACCTGAAGCAG

CTGCCCTTCTTCTACTACAGCGACAGCCCCTGCGAGAGCCACGGCAAG

CAGGTGGTGAGCGACATCGACTACGTGCCCCTGAAGAGCGCCACCTG

CATCACCAGGTGCAACCTGGGCGGCGCCGTGTGCAGGCACCACGCCA

ACGAGTACAGGCTGTACCTGGACGCCTACAACATGATGATCAGCGCCG

GCTTCAGCCTGTGGGTGTACAAGCAGTTCGACACCTACAACCTGTGGA

ACACCTTCACCAGGCTGCAGAGCCTGGAGAACGTGGCCTTCAACGTGG

TGAACAAGGGCCACTTCGACGGCCAGCAGGGCGAGGTGCCCGTGAGC

ATCATCAACAACACCGTGTACACCAAGGTGGACGGCGTGGACGTGGAG

CTGTTCGAGAACAAGACCACCCTGCCCGTGAACGTGGCCTTCGAGCTG

TGGGCCAAGAGGAACATCAAGCCCGTGCCCGAGGTGAAGATCCTGAAC

AACCTGGGCGTGGACATCGCCGCCAACACCGTGATCTGGGACTACAAG

AGGGACGCCCCCGCCCACATCAGCACCATCGGCGTGTGCAGCATGAC

CGACATCGCCAAGAAGCCCACCGAGACCATCTGCGCCCCCCTGACCGT

GTTCTTCGACGGCAGGGTGGACGGCCAGGTGGACCTGTTCAGGAACG

CCAGGAACGGCGTGCTGATCACCGAGGGCAGCGTGAAGGGCCTGCAG

CCCAGCGTGGGCCCCAAGCAGGCCAGCCTGAACGGCGTGACCCTGAT

CGGCGAGGCCGTGAAGACCCAGTTCAACTACTACAAGAAGGTGGACG

GCGTGGTGCAGCAGCTGCCCGAGACCTACTTCACCCAGAGCAGGAAC

CTGCAGGAGTTCAAGCCCAGGAGCCAGATGGAGATCGACTTCCTGGAG

CTGGCCATGGACGAGTTCATCGAGAGGTACAAGCTGGAGGGCTACGC

CTTCGAGCACATCGTGTACGGCGACTTCAGCCACAGCCAGCTGGGCG

GCCTGCACCTGCTGATCGGCCTGGCCAAGAGGTTCAAGGAGAGCCCC

TTCGAGCTGGAGGACTTCATCCCCATGGACAGCACCGTGAAGAACTAC

TTCATCACCGACGCCCAGACCGGCAGCAGCAAGTGCGTGTGCAGCGT

GATCGACCTGCTGCTGGACGACTTCGTGGAGATCATCAAGAGCCAGGA

CCTGAGCGTGGTGAGCAAGGTGGTGAAGGTGACCATCGACTACACCGA

GATCAGCTTCATGCTGTGGTGCAAGGACGGCCACGTGGAGACCTTCTA

CCCCAAGCTGCAGAGCAGCCAGGCCTGGCAGCCCGGCGTGGCCATGC

CCAACCTGTACAAGATGCAGAGGATGCTGCTGGAGAAGTGCGACCTGC

AGAACTACGGCGACAGCGCCACCCTGCCCAAGGGCATCATGATGAACG

TGGCCAAGTACACCCAGCTGTGCCAGTACCTGAACACCCTGACCCTGG

CCGTGCCCTACAACATGAGGGTGATCCACTTCGGCGCCGGCAGCGAC

AAGGGCGTGGCCCCCGGCACCGCCGTGCTGAGGCAGTGGCTGCCCAC

CGGCACCCTGCTGGTGGACAGCGACCTGAACGACTTCGTGAGCGACG

CCGACAGCACCCTGATCGGCGACTGCGCCACCGTGCACACCGCCAAC

AAGTGGGACCTGATCATCAGCGACATGTACGACCCCAAGACCAAGAAC

GTGACCAAGGAGAACGACAGCAAGGAGGGCTTCTTCACCTACATCTGC

GGCTTCATCCAGCAGAAGCTGGCCCTGGGCGGCAGCGTGGCCATCAA

GATCACCGAGCACAGCTGGAACGCCGACCTGTACAAGCTGATGGGCCA

CTTCGCCTGGTGGACCGCCTTCGTGACCAACGTGAACGCCAGCAGCAG

CGAGGCCTTCCTGATCGGCTGCAACTACCTGGGCAAGCCCAGGGAGC

AGATCGACGGCTACGTGATGCACGCCAACTACATCTTCTGGAGGAACA

CCAACCCCATCCAGCTGAGCAGCTACAGCCTGTTCGACATGAGCAAGT

TCCCCCTGAAGCTTGATGA

57
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGTG
342

Pan-CoV (B5)
AACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAGCTTC

ACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCT

GCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTG

GTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGA

CAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAG

CAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGAT

CAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTA

CTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTA

CAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCT

GATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGT

TCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACAC

CCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCCTGG

AGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGA

CCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGC

AGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCA

GCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGA

CGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGCACCC

TGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCA

GGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACC

TGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGT

ACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGC

GTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTG

AGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGAC

AGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCA

GACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCAC

CGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGG

GCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAGC

ACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTGCAG

AGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCTACAG

GGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGT

GCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACT

TCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACA

AGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCA

CCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACC

CCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACAC

CAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGG

TGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTG

TACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGAT

CGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCG

GCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCGGC

AGCGCCAGCAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAG

CCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCA

TCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGA

GCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACA

GCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCACCC

AGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACAAGAAC

ACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCCCCC

ATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCGACCCC

AGCAAGCCCAGCAAGAGGAGCCCCATCGAGGACCTGCTGTTCAACAAG

GTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCT

GGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACG

GCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAG

TACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTT

CGGCGCCGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATGGCCT

ACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACC

AGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGG

ACAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGTG

GTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAG

CAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAG

GCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCG

GCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGG

GCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAG

CGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGG

GCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTG

TTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACC

GCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGG

CGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTT

CTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAA

CTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCT

GCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCA

ACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAG

GTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGG

CAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTT

CATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTG

CATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGGCA

GCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGC

GTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCTGTTA

AAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTATGTACAGCTTC

GTGAGCGAGGAGACCGGCACCCTGATCGTGAACAGCGTGCTGCTGTT

CCTGGCCTTCGTGGTGTTCCTGCTGGTGACCCTGGCCATCCTGACCGC

CCTGAGGCTGTGCGCCTACTGCTGCAACATCGTGAACGTGAGCCTGGT

GAAGCCCAGCTTCTACGTGTACAGCAGGGTGAAGAACCTGAACAGCAG

CAGGGTGCCCGACCTGCTGGTGGGAAGCGGAGCCACGAACTTCTCTC

TGTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTATGGCCG

ACAGCAACGGCACCATCACCGTGGAGGAGCTGAAGAAGCTGCTGGAG

CAGTGGAACCTGGTGATCGGCTTCCTGTTCCTGACCTGGATCTGCCTG

CTGCAGTTCGCCTACGCCAACAGGAACAGGTTCCTGTACATCATCAAG

CTGATCTTCCTGTGGCTGCTGTGGCCCGTGACCCTGGCCTGCTTCGTG

CTGGCCGCCGTGTACAGGATCAACTGGATCACCGGCGGCATCGCCATC

GCCATGGCCTGCCTGGTGGGCCTGATGTGGCTGAGCTACTTCATCGCC

AGCTTCAGGCTGTTCGCCAGGACCAGGAGCATGTGGAGCTTCAACCCC

GAGACCAACATCCTGCTGAACGTGCCCCTGCACGGCACCATCCTGACC

AGGCCCCTGCTGGAGAGCGAGCTGGTGATCGGCGCCGTGATCCTGAG

GGGCCACCTGAGGATCGCCGGCCACCACCTGGGCAGGTGCGACATCA

AGGACCTGCCCAAGGAGATCACCGTGGCCACCAGCAGGACCCTGAGC

TACTACAAGCTGGGCGCCAGCCAGAGGGTGGCCGGCGACAGCGGCTT

CGCCGCCTACAGCAGGTACAGGATCGGCAACTACAAGCTGAACACCGA

CCACAGCAGCAGCAGCGACAACATCGCCCTGCTGGTGCAGGGAAGCG

GAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAAAA

CCCCGGGCCTATGAAGATCATCCTGTTCCTGGCCCTGATCACCCTGGC

CACCTGCGAGCTGTACCACTACCAGGAGTGCGTGAGGGGCACCACCG

TGTGATGA

58
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGTG
343

Pan-CoV (B6)
AACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAGCTTC

ACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCT

GCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTG

GTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGA

CAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAG

CAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGAT

CAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTA

CTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTA

CAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCT

GATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGT

TCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACAC

CCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCCTGG

AGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGA

CCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGC

AGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCA

GCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGA

CGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGCACCC

TGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCA

GGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACC

TGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGT

ACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGC

GTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTG

AGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGAC

AGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCA

GACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCAC

CGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGG

GCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAGC

ACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTGCAG

AGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCTACAG

GGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGT

GCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACT

TCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACA

AGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCA

CCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACC

CCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACAC

CAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGG

TGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTG

TACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGAT

CGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCG

GCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCGGC

AGCGCCAGCAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAG

CCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCA

TCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGA

GCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACA

GCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCACCC

AGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACAAGAAC

ACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCCCCC

ATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCGACCCC

AGCAAGCCCAGCAAGAGGAGCCCCATCGAGGACCTGCTGTTCAACAAG

GTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCT

GGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACG

GCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAG

TACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTT

CGGCGCCGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATGGCCT

ACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACC

AGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGG

ACAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGTG

GTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAG

CAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAG

GCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCG

GCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGG

GCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAG

CGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGG

GCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTG

TTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACC

GCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGG

CGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTT

CTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAA

CTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCT

GCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCA

ACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAG

GTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGG

CAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTT

CATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTG

CATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGGCA

GCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGC

GTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCTGTTA

AAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTGCCCCCACCAAG

GTGACCTTCGGCGACGACACCGTGATCGAGGTGCAGGGCTACAAGAG

CGTGAACATCACCTTCGAGCTGGACGAGAGGATCGACAAGGTGCTGAA

CGAGAAGTGCAGCGCCTACACCGTGGAGCTGGGCACCGAGGTGAACG

AGTTCGCCTGCGTGGTGGCCGACGCCGTGATCAAGACCCTGCAGCCC

GTGAGCGAGCTGCTGACCCCCCTGGGCATCGACCTGGACGAGTGGAG

CATGGCCACCTACTACCTGTTCGACGAGAGCGGCGAGTTCAAGCTGGC

CAGCCACATGTACTGCAGCTTCTACCCCCCCGACGAGGACGAGGAGGA

GGGCGACTGCGAGGAGGAGGAGTTCGAGCCCAGCACCCAGTACGAGT

ACGGCACCGAGGACGACTACCAGGGCAAGCCCCTGGAGTTCGGCGCC

ACCAGCGCCGCCCTGCAGCCCGAGGAGGAGCAGGAGGAGGACTGGC

TGGACGACGACAGCCAGCAGACCGTGGGCCAGCAGGACGGCAGCGA

GGACAACCAGACCACCACCATCCAGACCATCGTGGAGGTGCAGCCCCA

GCTGGAGATGGAGCTGACCCCCGTGGTGCAGACCATCGAGGTGAACA

GCTTCAGCGGCTACCTGAAGCTGACCGACAACGTGTACATCAAGAACG

CCGACATCGTGGAGGAGGCCAAGAAGGTGAAGCCCACCGTGGTGGTG

AACGCCGCCAACGTGTACCTGAAGCACGGCGGCGGCGTGGCCGGCGC

CCTGAACAAGGCCACCAACAACGCCATGCAGGTGGAGAGCGACGACTA

CATCGCCACCAACGGCCCCCTGAAGGTGGGCGGCAGCTGCGTGCTGA

GCGGCCACAACCTGGCCAAGCACTGCCTGCACGTGGTGGGCCCCAAC

GTGAACAAGGGCGAGGACATCCAGCTGCTGAAGAGCGCCTACGAGAA

CTTCAACCAGCACGAGGTGCTGCTGGCCCCCCTGCTGAGCGCCGGCA

TCTTCGGCGCCGACCCCATCCACAGCCTGAGGGTGTGCGTGGACACC

GTGAGGACCAACGTGTACCTGGCCGTGTTCGACAAGAACCTGTACGAC

AAGCTGGTGAGCAGCTTCCTGGAGATGAAGAGCGAGAAGCAGGTGGA

GCAGAAGATCGCCGAGATCCCCAAGGAGGAGGTGAAGCCCTTCATCAC

CGAGAGCAAGCCCAGCGTGGAGCAGAGGAAGCAGGACGACAAGAAGA

TCAAGGCCTGCGTGGAGGAGGTGACCACCACCCTGGAGGAGACCAAG

TTCCTGACCGAGAACCTGCTGCTGTACATCGACATCAACGGCAACCTG

CACCCCGACAGCGCCACCCTGGTGAGCGACATCGACATCACCTTCCTG

AAGAAGGACGCCCCCTACATCGTGGGCGACGTGGTGCAGGAGGGCGT

GCTGACCGCCGTGGTGATCCCCACCAAGAAGGCCGGCGGCACCACCG

AGATGCTGGCCAAGGCCCTGAGGAAGGTGCCCACCGACAACTACATCA

CCACCTACCCCGGCCAGGGCCTGAACGGCTACACCGTGGAGGAGGCC

AAGACCGTGCTGAAGAAGTGCAAGAGCGCCTTCTACATCCTGCCCAGC

ATCATCAGCAACGAGAAGCAGGAGATCCTGGGCACCGTGAGCTGGAAC

CTGAGGGAGATGCTGGCCCACGCCGAGGAGACCAGGAAGCTGATGCC

CGTGTGCGTGGAGACCAAGGCCATCGTGAGCACCATCCAGAGGAAGTA

CAAGGGCATCAAGATCCAGGAGGGCGTGGTGGACTACGGCGCCAGGT

TCTACTTCTACACCAGCAAGACCACCGTGGCCAGCCTGATCAACACCCT

GAACGACCTGAACGAGACCCTGGTGACCATGCCCCTGGGCTACGTGAC

CCACGGCCTGAACCTGGAGGAGGCCGCCAGGTACATGAGGAGCCTGA

AGGTGCCCGCCACCGTGAGCGTGAGCAGCCCCGACGCCGTGACCGCC

TACAACGGCTACCTGACCAGCAGCAGCAAGACCCCCGAGGAGCACTTC

ATCGAGACCATCAGCCTGGCCGGCAGCTACAAGGACTGGAGCTACAGC

GGCCAGAGCACCCAGCTGGGCATCGAGTTCCTGAAGAGGGGCGACAA

GAGCGTGTACTACACCAGCAACCCCACCACCTTCCACCTGGACGGCGA

GGTGATCACCTTCGACAACCTGAAGACCCTGCTGAGCCTGAGGGAGGT

GAGGACCATCAAGGTGTTCACCACCGTGGACAACATCAACCTGCACGG

AAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGA

AGAAAACCCCGGGCCTAGCGGCTTCAGGAAGATGGCCTTCCCCAGCG

GCAAGGTGGAGGGCTGCATGGTGCAGGTGACCTGCGGCACCACCACC

CTGAACGGCCTGTGGCTGGACGACGTGGTGTACTGCCCCAGGCACGT

GATCTGCACCAGCGAGGACATGCTGAACCCCAACTACGAGGACCTGCT

GATCAGGAAGAGCAACCACAACTTCCTGGTGCAGGCCGGCAACGTGCA

GCTGAGGGTGATCGGCCACAGCATGCAGAACTGCGTGCTGAAGCTGAA

GGTGGACACCGCCAACCCCAAGACCCCCAAGTACAAGTTCGTGAGGAT

CCAGCCCGGCCAGACCTTCAGCGTGCTGGCCTGCTACAACGGCAGCC

CCAGCGGCGTGTACCAGTGCGCCATGAGGCCCAACTTCACCATCAAGG

GCAGCTTCCTGAACGGCAGCTGCGGCAGCGTGGGCTTCAACATCGACT

ACGACTGCGTGAGCTTCTGCTACATGCACCACATGGAGCTGCCCACCG

GCGTGCACGCCGGCACCGACCTGGAGGGCAACTTCTACGGCCCCTTC

GTGGACAGGCAGACCGCCCAGGCCGCCGGCACCGACACCACCATCAC

CGTGAACGTGCTGGCCTGGCTGTACGCCGCCGTGATCAACGGCGACA

GGTGGTTCCTGAACAGGTTCACCACCACCCTGAACGACTTCAACCTGG

TGGCCATGAAGTACAACTACGAGCCCCTGACCCAGGACCACGTGGACA

TCCTGGGCCCCCTGAGCGCCCAGACCGGCATCGCCGTGCTGGACATG

TGCGCCAGCCTGAAGGAGCTGCTGCAGAACGGCATGAACGGCAGGAC

CATCCTGGGCAGCGCCCTGCTGGAGGACGAGTTCACCCCCTTCGACGT

GGTGAGGCAGTGCAGCGGCGTGACCTTCCAGGGAAGCGGAGCCACGA

ACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGC

CTAGCGCCGACGCCCAGAGCTTCCTGAACAGGGTGTGCGGCGTGAGC

GCCGCCAGGCTGACCCCCTGCGGCACCGGCACCAGCACCGACGTGGT

GTACAGGGCCTTCGACATCTACAACGACAAGGTGGCCGGCTTCGCCAA

GTTCCTGAAGACCAACTGCTGCAGGTTCCAGGAGAAGGACGAGGACGA

CAACCTGATCGACAGCTACTTCGTGGTGAAGAGGCACACCTTCAGCAA

CTACCAGCACGAGGAGACCATCTACAACCTGCTGAAGGACTGCCCCGC

CGTGGCCAAGCACGACTTCTTCAAGTTCAGGATCGACGGCGACATGGT

GCCCCACATCAGCAGGCAGAGGCTGACCAAGTACACCATGGCCGACCT

GGTGTACGCCCTGAGGCACTTCGACGAGGGCAACTGCGACACCCTGA

AGGAGATCCTGGTGACCTACAACTGCTGCGACGACGACTACTTCAACA

AGAAGGACTGGTACGACTTCGTGGAGAACCCCGACATCCTGAGGGTGT

ACGCCAACCTGGGCGAGAGGGTGAGGCAGGCCCTGCTGAAGACCGTG

CAGTTCTGCGACGCCATGAGGAACGCCGGCATCGTGGGCGTGCTGAC

CCTGGACAACCAGGACCTGAACGGCAACTGGTACGACTTCGGCGACTT

CATCCAGACCACCCCCGGCAGCGGCGTGCCCGTGGTGGACAGCTACT

ACAGCCTGCTGATGCCCATCCTGACCCTGACCAGGGCCCTGACCGCC

GAGAGCCACGTGGACACCGACCTGACCAAGCCCTACATCAAGTGGGAC

CTGCTGAAGTACGACTTCACCGAGGAGAGGCTGAAGCTGTTCGACAGG

TACTTCAAGTACTGGGACCAGACCTACCACCCCAACTGCGTGAACTGC

CTGGACGACAGGTGCATCCTGCACTGCGCCAACTTCAACGTGCTGTTC

AGCACCGTGTTCCCCCCCACCAGCTTCGGCCCCCTGGTGAGGAAGATC

TTCGTGGACGGCGTGCCCTTCGTGGTGAGCACCGGCTACCACTTCAGG

GAGCTGGGCGTGGTGCACAACCAGGACGTGAACCTGCACAGCAGCAG

GCTGAGCTTCAAGGAGCTGCTGGTGTACGCCGCCGACCCCGCCATGC

ACGCCGCCAGCGGCAACCTGCTGCTGGACAAGAGGACCACCTGCTTC

AGCGTGGCCGCCCTGACCAACAACGTGGCCTTCCAGACCGTGAAGCC

CGGCAACTTCAACAAGGACTTCTACGACTTCGCCGTGAGCAAGGGCTT

CTTCAAGGAGGGCAGCAGCGTGGAGCTGAAGCACTTCTTCTTCGCCCA

GGACGGCAACGCCGCCATCAGCGACTACGACTACTACAGGTACAACCT

GCCCACCATGTGCGACATCAGGCAGCTGCTGTTCGTGGTGGAGGTGGT

GGACAAGTACTTCGACTGCTACGACGGCGGCTGCATCAACGCCAACCA

GGTGATCGTGAACAACCTGGACAAGAGCGCCGGCTTCCCCTTCAACAA

GTGGGGCAAGGCCAGGCTGTACTACGACAGCATGAGCTACGAGGACC

AGGACGCCCTGTTCGCCTACACCAAGAGGAACGTGATCCCCACCATCA

CCCAGATGAACCTGAAGTACGCCATCAGCGCCAAGAACAGGGCCAGGA

CCGTGGCCGGCGTGAGCATCTGCAGCACCATGACCAACAGGCAGTTC

CACCAGAAGCTGCTGAAGAGCATCGCCGCCACCAGGGGCGCCACCGT

GGTGATCGGCACCAGCAAGTTCTACGGCGGCTGGCACAACATGCTGAA

GACCGTGTACAGCGACGTGGAGAACCCCCACCTGATGGGCTGGGACT

ACCCCAAGTGCGACAGGGCCATGCCCAACATGCTGAGGATCATGGCCA

GCCTGGTGCTGGCCAGGAAGCACACCACCTGCTGCAGCCTGAGCCAC

AGGTTCTACAGGCTGGCCAACGAGTGCGCCCAGGTGCTGAGCGAGAT

GGTGATGTGCGGCGGCAGCCTGTACGTGAAGCCCGGCGGCACCAGCA

GCGGCGACGCCACCACCGCCTACGCCAACAGCGTGTTCAACATCTGCC

AGGCCGTGACCGCCAACGTGAACGCCCTGCTGAGCACCGACGGCAAC

AAGATCGCCGACAAGTACGTGAGGAACCTGCAGCACAGGCTGTACGAG

TGCCTGTACAGGAACAGGGACGTGGACACCGACTTCGTGAACGAGTTC

TACGCCTACCTGAGGAAGCACTTCAGCATGATGATCCTGAGCGACGAC

GCCGTGGTGTGCTTCAACAGCACCTACGCCAGCCAGGGCCTGGTGGC

CAGCATCAAGAACTTCAAGAGCGTGCTGTACTACCAGAACAACGTGTTC

ATGAGCGAGGCCAAGTGCTGGACCGAGACCGACCTGACCAAGGGCCC

CCACGAGTTCTGCAGCCAGCACACCATGCTGGTGAAGCAGGGCGACG

ACTACGTGTACCTGCCCTACCCCGACCCCAGCAGGATCCTGGGCGCC

GGCTGCTTCGTGGACGACATCGTGAAGACCGACGGCACCCTGATGATC

GAGAGGTTCGTGAGCCTGGCCATCGACGCCTACCCCCTGACCAAGCAC

CCCAACCAGGAGTACGCCGACGTGTTCCACCTGTACCTGCAGTACATC

AGGAAGCTGCACGACGAGCTGACCGGCCACATGCTGGACATGTACAG

CGTGATGCTGACCAACGACAACACCAGCAGGTACTGGGAGCCCGAGTT

CTACGAGGCCATGTACACCCCCCACACCGTGCTGCAGTGATGA

59
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGTG
344

Pan-CoV
AACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAGCTTC

(B6.1)
ACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCT

GCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTG

GTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGA

CAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAG

CAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGAT

CAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTA

CTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTA

CAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCT

GATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGT

TCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACAC

CCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCCTGG

AGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGA

CCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGC

AGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCA

GCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGA

CGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGCACCC

TGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCA

GGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACC

TGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGT

ACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGC

GTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTG

AGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGAC

AGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCA

GACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCAC

CGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGG

GCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAGC

ACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTGCAG

AGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCTACAG

GGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGT

GCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACT

TCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACA

AGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCA

CCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACC

CCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACAC

CAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGG

TGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTG

TACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGAT

CGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCG

GCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCGGC

AGCGCCAGCAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAG

CCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCA

TCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGA

GCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACA

GCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCACCC

AGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACAAGAAC

ACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCCCCC

ATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCGACCCC

AGCAAGCCCAGCAAGAGGAGCCCCATCGAGGACCTGCTGTTCAACAAG

GTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCT

GGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACG

GCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAG

TACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTT

CGGCGCCGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATGGCCT

ACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACC

AGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGG

ACAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGTG

GTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAG

CAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAG

GCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCG

GCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGG

GCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAG

CGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGG

GCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTG

TTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACC

GCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGG

CGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTT

CTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAA

CTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCT

GCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCA

ACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAG

GTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGG

CAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTT

CATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTG

CATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGGCA

GCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGC

GTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCTGTTA

AAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTGCCCCCACCAAG

GTGACCTTCGGCGACGACACCGTGATCGAGGTGCAGGGCTACAAGAG

CGTGAACATCACCTTCGAGCTGGACGAGAGGATCGACAAGGTGCTGAA

CGAGAAGTGCAGCGCCTACACCGTGGAGCTGGGCACCGAGGTGAACG

AGTTCGCCTGCGTGGTGGCCGACGCCGTGATCAAGACCCTGCAGCCC

GTGAGCGAGCTGCTGACCCCCCTGGGCATCGACCTGGACGAGTGGAG

CATGGCCACCTACTACCTGTTCGACGAGAGCGGCGAGTTCAAGCTGGC

CAGCCACATGTACTGCAGCTTCTACCCCCCCGACGAGGACGAGGAGGA

GGGCGACTGCGAGGAGGAGGAGTTCGAGCCCAGCACCCAGTACGAGT

ACGGCACCGAGGACGACTACCAGGGCAAGCCCCTGGAGTTCGGCGCC

ACCAGCGCCGCCCTGCAGCCCGAGGAGGAGCAGGAGGAGGACTGGC

TGGACGACGACAGCCAGCAGACCGTGGGCCAGCAGGACGGCAGCGA

GGACAACCAGACCACCACCATCCAGACCATCGTGGAGGTGCAGCCCCA

GCTGGAGATGGAGCTGACCCCCGTGGTGCAGACCATCGAGGTGAACA

GCTTCAGCGGCTACCTGAAGCTGACCGACAACGTGTACATCAAGAACG

CCGACATCGTGGAGGAGGCCAAGAAGGTGAAGCCCACCGTGGTGGTG

AACGCCGCCAACGTGTACCTGAAGCACGGCGGCGGCGTGGCCGGCGC

CCTGAACAAGGCCACCAACAACGCCATGCAGGTGGAGAGCGACGACTA

CATCGCCACCAACGGCCCCCTGAAGGTGGGCGGCAGCTGCGTGCTGA

GCGGCCACAACCTGGCCAAGCACTGCCTGCACGTGGTGGGCCCCAAC

GTGAACAAGGGCGAGGACATCCAGCTGCTGAAGAGCGCCTACGAGAA

CTTCAACCAGCACGAGGTGCTGCTGGCCCCCCTGCTGAGCGCCGGCA

TCTTCGGCGCCGACCCCATCCACAGCCTGAGGGTGTGCGTGGACACC

GTGAGGACCAACGTGTACCTGGCCGTGTTCGACAAGAACCTGTACGAC

AAGCTGGTGAGCAGCTTCCTGGAGATGAAGAGCGAGAAGCAGGTGGA

GCAGAAGATCGCCGAGATCCCCAAGGAGGAGGTGAAGCCCTTCATCAC

CGAGAGCAAGCCCAGCGTGGAGCAGAGGAAGCAGGACGACAAGAAGA

TCAAGGCCTGCGTGGAGGAGGTGACCACCACCCTGGAGGAGACCAAG

TTCCTGACCGAGAACCTGCTGCTGTACATCGACATCAACGGCAACCTG

CACCCCGACAGCGCCACCCTGGTGAGCGACATCGACATCACCTTCCTG

AAGAAGGACGCCCCCTACATCGTGGGCGACGTGGTGCAGGAGGGCGT

GCTGACCGCCGTGGTGATCCCCACCAAGAAGGCCGGCGGCACCACCG

AGATGCTGGCCAAGGCCCTGAGGAAGGTGCCCACCGACAACTACATCA

CCACCTACCCCGGCCAGGGCCTGAACGGCTACACCGTGGAGGAGGCC

AAGACCGTGCTGAAGAAGTGCAAGAGCGCCTTCTACATCCTGCCCAGC

ATCATCAGCAACGAGAAGCAGGAGATCCTGGGCACCGTGAGCTGGAAC

CTGAGGGAGATGCTGGCCCACGCCGAGGAGACCAGGAAGCTGATGCC

CGTGTGCGTGGAGACCAAGGCCATCGTGAGCACCATCCAGAGGAAGTA

CAAGGGCATCAAGATCCAGGAGGGCGTGGTGGACTACGGCGCCAGGT

TCTACTTCTACACCAGCAAGACCACCGTGGCCAGCCTGATCAACACCCT

GAACGACCTGAACGAGACCCTGGTGACCATGCCCCTGGGCTACGTGAC

CCACGGCCTGAACCTGGAGGAGGCCGCCAGGTACATGAGGAGCCTGA

AGGTGCCCGCCACCGTGAGCGTGAGCAGCCCCGACGCCGTGACCGCC

TACAACGGCTACCTGACCAGCAGCAGCAAGACCCCCGAGGAGCACTTC

ATCGAGACCATCAGCCTGGCCGGCAGCTACAAGGACTGGAGCTACAGC

GGCCAGAGCACCCAGCTGGGCATCGAGTTCCTGAAGAGGGGCGACAA

GAGCGTGTACTACACCAGCAACCCCACCACCTTCCACCTGGACGGCGA

GGTGATCACCTTCGACAACCTGAAGACCCTGCTGAGCCTGAGGGAGGT

GAGGACCATCAAGGTGTTCACCACCGTGGACAACATCAACCTGCACTG

ATGA

60
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGTG
345

Pan-CoV
AACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAGCTTC

(B6.2)
ACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCT

GCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTG

GTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGA

CAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAG

CAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGAT

CAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTA

CTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTA

CAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCT

GATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGT

TCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACAC

CCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCCTGG

AGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGA

CCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGC

AGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCA

GCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGA

CGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGCACCC

TGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCA

GGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACC

TGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGT

ACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGC

GTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTG

AGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGAC

AGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCA

GACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCAC

CGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGG

GCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAGC

ACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTGCAG

AGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCTACAG

GGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGT

GCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACT

TCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACA

AGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCA

CCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACC

CCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACAC

CAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGG

TGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTG

TACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGAT

CGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCG

GCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCGGC

AGCGCCAGCAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAG

CCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCA

TCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGA

GCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACA

GCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCACCC

AGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACAAGAAC

ACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCCCCC

ATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCGACCCC

AGCAAGCCCAGCAAGAGGAGCCCCATCGAGGACCTGCTGTTCAACAAG

GTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCT

GGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACG

GCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAG

TACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTT

CGGCGCCGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATGGCCT

ACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACC

AGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGG

ACAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGTG

GTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAG

CAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAG

GCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCG

GCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGG

GCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAG

CGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGG

GCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTG

TTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACC

GCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGG

CGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTT

CTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAA

CTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCT

GCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCA

ACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAG

GTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGG

CAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTT

CATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTG

CATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGGCA

GCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGC

GTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCTGTTA

AAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTAGCGGCTTCAGG

AAGATGGCCTTCCCCAGCGGCAAGGTGGAGGGCTGCATGGTGCAGGT

GACCTGCGGCACCACCACCCTGAACGGCCTGTGGCTGGACGACGTGG

TGTACTGCCCCAGGCACGTGATCTGCACCAGCGAGGACATGCTGAACC

CCAACTACGAGGACCTGCTGATCAGGAAGAGCAACCACAACTTCCTGG

TGCAGGCCGGCAACGTGCAGCTGAGGGTGATCGGCCACAGCATGCAG

AACTGCGTGCTGAAGCTGAAGGTGGACACCGCCAACCCCAAGACCCCC

AAGTACAAGTTCGTGAGGATCCAGCCCGGCCAGACCTTCAGCGTGCTG

GCCTGCTACAACGGCAGCCCCAGCGGCGTGTACCAGTGCGCCATGAG

GCCCAACTTCACCATCAAGGGCAGCTTCCTGAACGGCAGCTGCGGCAG

CGTGGGCTTCAACATCGACTACGACTGCGTGAGCTTCTGCTACATGCA

CCACATGGAGCTGCCCACCGGCGTGCACGCCGGCACCGACCTGGAGG

GCAACTTCTACGGCCCCTTCGTGGACAGGCAGACCGCCCAGGCCGCC

GGCACCGACACCACCATCACCGTGAACGTGCTGGCCTGGCTGTACGC

CGCCGTGATCAACGGCGACAGGTGGTTCCTGAACAGGTTCACCACCAC

CCTGAACGACTTCAACCTGGTGGCCATGAAGTACAACTACGAGCCCCT

GACCCAGGACCACGTGGACATCCTGGGCCCCCTGAGCGCCCAGACCG

GCATCGCCGTGCTGGACATGTGCGCCAGCCTGAAGGAGCTGCTGCAG

AACGGCATGAACGGCAGGACCATCCTGGGCAGCGCCCTGCTGGAGGA

CGAGTTCACCCCCTTCGACGTGGTGAGGCAGTGCAGCGGCGTGACCTT

CCAGTGATGA

61
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGTG
346

Pan-CoV
AACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAGCTTC

(B6.3)
ACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCT

GCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTG

GTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGA

CAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAG

CAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGAT

CAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTA

CTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTA

CAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCT

GATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGT

TCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACAC

CCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCCTGG

AGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGA

CCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGC

AGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCA

GCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACCGA

CGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGCACCC

TGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCA

GGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACATCACCAACC

TGTGCCCCTTCGGCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGT

ACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGC

GTGCTGTACAACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTG

AGCCCCACCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGAC

AGCTTCGTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCA

GACCGGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCAC

CGGCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGG

GCGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAGC

ACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTGCAG

AGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCTACAG

GGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGT

GCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACT

TCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACA

AGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCA

CCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACC

CCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACAC

CAGCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGG

TGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTG

TACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGAT

CGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCG

GCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCGGC

AGCGCCAGCAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAG

CCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCA

TCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGA

GCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACA

GCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCACCC

AGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACAAGAAC

ACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACCCCCCCC

ATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCGACCCC

AGCAAGCCCAGCAAGAGGAGCCCCATCGAGGACCTGCTGTTCAACAAG

GTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCT

GGGCGACATCGCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACG

GCCTGACCGTGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAG

TACACCAGCGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTT

CGGCGCCGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATGGCCT

ACAGGTTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACC

AGAAGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGG

ACAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGTG

GTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAG

CAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAG

GCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCG

GCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGG

GCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAG

CGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGG

GCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTG

TTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACC

GCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGG

CGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTT

CTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAA

CTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCT

GCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCA

ACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAG

GTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGG

CAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTT

CATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCTG

CATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGGCA

GCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAGGGC

GTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTCTGTTA

AAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTAGCGCCGACGC

CCAGAGCTTCCTGAACAGGGTGTGCGGCGTGAGCGCCGCCAGGCTGA

CCCCCTGCGGCACCGGCACCAGCACCGACGTGGTGTACAGGGCCTTC

GACATCTACAACGACAAGGTGGCCGGCTTCGCCAAGTTCCTGAAGACC

AACTGCTGCAGGTTCCAGGAGAAGGACGAGGACGACAACCTGATCGAC

AGCTACTTCGTGGTGAAGAGGCACACCTTCAGCAACTACCAGCACGAG

GAGACCATCTACAACCTGCTGAAGGACTGCCCCGCCGTGGCCAAGCAC

GACTTCTTCAAGTTCAGGATCGACGGCGACATGGTGCCCCACATCAGC

AGGCAGAGGCTGACCAAGTACACCATGGCCGACCTGGTGTACGCCCT

GAGGCACTTCGACGAGGGCAACTGCGACACCCTGAAGGAGATCCTGG

TGACCTACAACTGCTGCGACGACGACTACTTCAACAAGAAGGACTGGT

ACGACTTCGTGGAGAACCCCGACATCCTGAGGGTGTACGCCAACCTGG

GCGAGAGGGTGAGGCAGGCCCTGCTGAAGACCGTGCAGTTCTGCGAC

GCCATGAGGAACGCCGGCATCGTGGGCGTGCTGACCCTGGACAACCA

GGACCTGAACGGCAACTGGTACGACTTCGGCGACTTCATCCAGACCAC

CCCCGGCAGCGGCGTGCCCGTGGTGGACAGCTACTACAGCCTGCTGA

TGCCCATCCTGACCCTGACCAGGGCCCTGACCGCCGAGAGCCACGTG

GACACCGACCTGACCAAGCCCTACATCAAGTGGGACCTGCTGAAGTAC

GACTTCACCGAGGAGAGGCTGAAGCTGTTCGACAGGTACTTCAAGTAC

TGGGACCAGACCTACCACCCCAACTGCGTGAACTGCCTGGACGACAGG

TGCATCCTGCACTGCGCCAACTTCAACGTGCTGTTCAGCACCGTGTTCC

CCCCCACCAGCTTCGGCCCCCTGGTGAGGAAGATCTTCGTGGACGGC

GTGCCCTTCGTGGTGAGCACCGGCTACCACTTCAGGGAGCTGGGCGT

GGTGCACAACCAGGACGTGAACCTGCACAGCAGCAGGCTGAGCTTCAA

GGAGCTGCTGGTGTACGCCGCCGACCCCGCCATGCACGCCGCCAGCG

GCAACCTGCTGCTGGACAAGAGGACCACCTGCTTCAGCGTGGCCGCC

CTGACCAACAACGTGGCCTTCCAGACCGTGAAGCCCGGCAACTTCAAC

AAGGACTTCTACGACTTCGCCGTGAGCAAGGGCTTCTTCAAGGAGGGC

AGCAGCGTGGAGCTGAAGCACTTCTTCTTCGCCCAGGACGGCAACGCC

GCCATCAGCGACTACGACTACTACAGGTACAACCTGCCCACCATGTGC

GACATCAGGCAGCTGCTGTTCGTGGTGGAGGTGGTGGACAAGTACTTC

GACTGCTACGACGGCGGCTGCATCAACGCCAACCAGGTGATCGTGAAC

AACCTGGACAAGAGCGCCGGCTTCCCCTTCAACAAGTGGGGCAAGGC

CAGGCTGTACTACGACAGCATGAGCTACGAGGACCAGGACGCCCTGTT

CGCCTACACCAAGAGGAACGTGATCCCCACCATCACCCAGATGAACCT

GAAGTACGCCATCAGCGCCAAGAACAGGGCCAGGACCGTGGCCGGCG

TGAGCATCTGCAGCACCATGACCAACAGGCAGTTCCACCAGAAGCTGC

TGAAGAGCATCGCCGCCACCAGGGGCGCCACCGTGGTGATCGGCACC

AGCAAGTTCTACGGCGGCTGGCACAACATGCTGAAGACCGTGTACAGC

GACGTGGAGAACCCCCACCTGATGGGCTGGGACTACCCCAAGTGCGA

CAGGGCCATGCCCAACATGCTGAGGATCATGGCCAGCCTGGTGCTGG

CCAGGAAGCACACCACCTGCTGCAGCCTGAGCCACAGGTTCTACAGGC

TGGCCAACGAGTGCGCCCAGGTGCTGAGCGAGATGGTGATGTGCGGC

GGCAGCCTGTACGTGAAGCCCGGCGGCACCAGCAGCGGCGACGCCAC

CACCGCCTACGCCAACAGCGTGTTCAACATCTGCCAGGCCGTGACCGC

CAACGTGAACGCCCTGCTGAGCACCGACGGCAACAAGATCGCCGACAA

GTACGTGAGGAACCTGCAGCACAGGCTGTACGAGTGCCTGTACAGGAA

CAGGGACGTGGACACCGACTTCGTGAACGAGTTCTACGCCTACCTGAG

GAAGCACTTCAGCATGATGATCCTGAGCGACGACGCCGTGGTGTGCTT

CAACAGCACCTACGCCAGCCAGGGCCTGGTGGCCAGCATCAAGAACTT

CAAGAGCGTGCTGTACTACCAGAACAACGTGTTCATGAGCGAGGCCAA

GTGCTGGACCGAGACCGACCTGACCAAGGGCCCCCACGAGTTCTGCA

GCCAGCACACCATGCTGGTGAAGCAGGGCGACGACTACGTGTACCTG

CCCTACCCCGACCCCAGCAGGATCCTGGGCGCCGGCTGCTTCGTGGA

CGACATCGTGAAGACCGACGGCACCCTGATGATCGAGAGGTTCGTGAG

CCTGGCCATCGACGCCTACCCCCTGACCAAGCACCCCAACCAGGAGTA

CGCCGACGTGTTCCACCTGTACCTGCAGTACATCAGGAAGCTGCACGA

CGAGCTGACCGGCCACATGCTGGACATGTACAGCGTGATGCTGACCAA

CGACAACACCAGCAGGTACTGGGAGCCCGAGTTCTACGAGGCCATGTA

CACCCCCCACACCGTGCTGCAGTGATGA

62
ATGAGCGACAACGGCCCCCAGAACCAGAGGAACGCCCCCAGGATCAC
347

Pan-CoV (B3)
CTTCGGCGGCCCCAGCGACAGCACCGGCAGCAACCAGAACGGCGAGA

GGAGCGGCGCCAGGAGCAAGCAGAGGAGGCCCCAGGGCCTGCCCAA

CAACACCGCCAGCTGGTTCACCGCCCTGACCCAGCACGGCAAGGAGG

ACCTGAAGTTCCCCAGGGGCCAGGGCGTGCCCATCAACACCAACAGCA

GCCCCGACGACCAGATCGGCTACTACAGGAGGGCCACCAGGAGGATC

AGGGGCGGCGACGGCAAGATGAAGGACCTGAGCCCCAGGTGGTACTT

CTACTACCTGGGCACCGGCCCCGAGGCCGGCCTGCCCTACGGCGCCA

ACAAGGACGGCATCATCTGGGTGGCCACCGAGGGCGCCCTGAACACC

CCCAAGGACCACATCGGCACCAGGAACCCCGCCAACAACGCCGCCAT

CGTGCTGCAGCTGCCCCAGGGCACCACCCTGCCCAAGGGCTTCTACG

CCGAGGGCAGCAGGGGCGGCAGCCAGGCCAGCAGCAGGAGCAGCAG

CAGGAGCAGGAACAGCAGCAGGAACAGCACCCCCGGCAGCAGCAGGG

GCACCAGCCCCGCCAGGATGGCCGGCAACGGCGGCGACGCCGCCCT

GGCCCTGCTGCTGCTGGACAGGCTGAACCAGCTGGAGAGCAAGATGA

GCGGCAAGGGCCAGCAGCAGCAGGGCCAGACCGTGACCAAGAAGAGC

GCCGCCGAGGCCAGCAAGAAGCCCAGGCAGAAGAGGACCGCCACCAA

GGCCTACAACGTGACCCAGGCCTTCGGCAGGAGGGGCCCCGAGCAGA

CCCAGGGCAACTTCGGCGACCAGGAGCTGATCAGGCAGGGCACCGAC

TACAAGCACTGGCCCCAGATCGCCCAGTTCGCCCCCAGCGCCAGCGC

CTTCTTCGGCATGAGCAGGATCGGCATGGAGGTGACCCCCAGCGGCA

CCTGGCTGACCTACACCGGCGCCATCAAGCTGGACGACAAGGACCCC

AACTTCAAGGACCAGGTGATCCTGCTGAACAAGCACATCGACGCCTAC

AAGACCTTCCCCCCCACCGAGCCCAAGAAGGACAAGAAGAAGAAGGCC

GACGAGACCCAGGCCCTGCCCCAGAGGCAGAAGAAGCAGCAGACCGT

GACCCTGCTGCCCGCCGCCGACCTGGACGACTTCAGCAAGCAGCTGC

AGCAGAGCATGAGCAGCGCCGACAGCACCCAGGCCTGATGA

As mentioned above, the present invention is not limited to the examples in Table 9. In some embodiments, vaccine candidates may comprise various pieces (e.g. promoters, proteins, adjuvants) as shown described herein.

The present invention may further feature a pan-coronavirus recombinant vaccine composition, the composition comprising one or more large sequences, wherein each of the one or more large sequences comprise conserved regions of a coronavirus. For example, Table 10 features non-limiting examples of proteins comprising conserved region from a coronavirus that may be used in the compositions described herein.

Table 10 shows non-limiting examples of proteins that may be used to create a vaccine composition described herein. In some embodiments, proteins listed below may be arranged in a plurality of combinations. In some embodiments, the proteins may be directly linked together. In other embodiments, the proteins are linked together via a linker.

TABLET0

SEQ ID

Proteins
Sequence
NO:

Spike glycoprotein
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTG
148

with 6 stabilizing
CGTGAACCTGACCACCCGGACCCAGCTGCCACCAGCCTACACC

mutations
AACAGCTTCACCCGGGGCGTCTACTACCCCGACAAGGTGTTCCG

(HexaPro)
GAGCAGCGTCCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCT

TCAGCAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCAC

CAACGGCACCAAGCGGTTCGACAACCCCGTGCTGCCCTTCAAC

GACGGCGTGTACTTCGCCAGCACCGAGAAGAGCAACATCATCC

GGGGCTGGATCTTCGGCACCACCCTGGACAGCAAGACCCAGAG

CCTGCTGATCGTGAATAACGCCACCAACGTGGTGATCAAGGTGT

GCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTACTAC

CACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCCGGGTGT

ACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCC

CTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACC

TGCGGGAGTTCGTGTTCAAGAACATCGACGGCTACTTCAAGATC

TACAGCAAGCACACCCCAATCAACCTGGTGCGGGATCTGCCCCA

GGGCTTCTCAGCCCTGGAGCCCCTGGTGGACCTGCCCATCGGC

ATCAACATCACCCGGTTCCAGACCCTGCTGGCCCTGCACCGGAG

CTACCTGACCCCAGGCGACAGCAGCAGCGGGTGGACAGCAGGC

GCGGCTGCTTACTACGTGGGCTACCTGCAGCCCCGGACCTTCCT

GCTGAAGTACAACGAGAACGGCACCATCACCGACGCCGTGGAC

TGCGCCCTGGACCCTCTGAGCGAGACCAAGTGCACCCTGAAGA

GCTTCACCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCCGG

GTGCAGCCCACCGAGAGCATCGTGCGGTTCCCCAACATCACCAA

CCTGTGCCCCTTCGGCGAGGTGTTCAACGCCACCCGGTTCGCC

AGCGTGTACGCCTGGAACCGGAAGCGGATCAGCAACTGCGTGG

CCGACTACAGCGTGCTGTACAACAGCGCCAGCTTCAGCACCTTC

AAGTGCTACGGCGTGAGCCCCACCAAGCTGAACGACCTGTGCTT

CACCAACGTGTACGCCGACAGCTTCGTGATCCGTGGCGACGAG

GTGCGGCAGATCGCACCCGGCCAGACAGGCAAGATCGCCGACT

ACAACTACAAGCTGCCCGACGACTTCACCGGCTGCGTGATCGCC

TGGAACAGCAACAACCTCGACAGCAAGGTGGGCGGCAACTACA

ACTACCTGTACCGGCTGTTCCGGAAGAGCAACCTGAAGCCCTTC

GAGCGGGACATCAGCACCGAGATCTACCAAGCCGGCTCCACCC

CTTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCTCTGCAG

AGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCT

ACCGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCAGC

CACCGTGTGTGGCCCCAAGAAGAGCACCAACCTGGTGAAGAAC

AAGTGCGTGAACTTCAACTTCAACGGCCTTACCGGCACCGGCGT

GCTGACCGAGAGCAACAAGAAATTCCTGCCCTTTCAGCAGTTCG

GCCGGGACATCGCCGACACCACCGACGCTGTGCGGGATCCCCA

GACCCTGGAGATCCTGGACATCACCCCTTGCAGCTTCGGCGGC

GTGAGCGTGATCACCCCAGGCACCAACACCAGCAACCAGGTGG

CCGTGCTGTACCAGGACGTGAACTGCACCGAGGTGCCCGTGGC

CATCCACGCCGACCAGCTGACACCCACCTGGCGGGTCTACAGC

ACCGGCAGCAACGTGTTCCAGACCCGGGCCGGTTGCCTGATCG

GCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCAT

CGGCGCCGGCATCTGTGCCAGCTACCAGACCCAGACCAATTCA

CCCGGCAGCGCCAGCAGCGTGGCCAGCCAGAGCATCATCGCCT

ACACCATGAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAA

CAACAGCATCGCCATCCCCACCAACTTCACCATCAGCGTGACCA

CCGAGATTCTGCCCGTGAGCATGACCAAGACCAGCGTGGACTG

CACCATGTACATCTGCGGCGACAGCACCGAGTGCAGCAACCTG

CTGCTGCAGTACGGCAGCTTCTGCACCCAGCTGAACCGGGCCC

TGACCGGCATCGCCGTGGAGCAGGACAAGAACACCCAGGAGGT

GTTCGCCCAGGTGAAGCAGATCTACAAGACCCCTCCCATCAAGG

ACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCGACCCCAGC

AAGCCCAGCAAGCGGAGCCCCATCGAGGACCTGCTGTTCAACA

AGGTGACCCTAGCCGACGCCGGCTTCATCAAGCAGTACGGCGA

CTGCCTCGGCGACATAGCCGCCCGGGACCTGATCTGCGCCCAG

AAGTTCAACGGCCTGACCGTGCTGCCTCCCCTGCTGACCGACGA

GATGATCGCCCAGTACACCAGCGCCCTGTTAGCCGGAACCATCA

CCAGCGGCTGGACTTTCGGCGCTGGCCCCGCTCTGCAGATCCC

CTTCCCCATGCAGATGGCCTACCGGTTCAACGGCATCGGCGTGA

CCCAGAACGTGCTGTACGAGAACCAGAAGCTGATCGCCAACCAG

TTCAACAGCGCCATCGGCAAGATCCAGGACAGCCTGAGCAGCA

CCCCTAGCGCCCTGGGCAAGCTGCAGGACGTGGTGAACCAGAA

CGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAGCAGCAAC

TTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCCGGC

TGGACCCTCCCGAGGCCGAGGTGCAGATCGACCGGCTGATCAC

TGGCCGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTG

ATCCGGGCCGCCGAGATTCGGGCCAGCGCCAACCTGGCCGCCA

CCAAGATGAGCGAGTGCGTGCTGGGCCAGAGCAAGCGGGTGGA

CTTCTGCGGCAAGGGCTACCACCTGATGAGCTTTCCCCAGAGCG

CACCCCACGGAGTGGTGTTCCTGCACGTGACCTACGTGCCCGC

CCAGGAGAAGAACTTCACCACCGCCCCAGCCATCTGCCACGAC

GGCAAGGCCCACTTTCCCCGGGAGGGCGTGTTCGTGAGCAACG

GCACCCACTGGTTCGTGACCCAGCGGAACTTCTACGAGCCCCA

GATCATCACCACCGACAACACCTTCGTGAGCGGCAACTGCGACG

TGGTGATCGGCATCGTGAACAACACCGTGTACGATCCCCTGCAG

CCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAATCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGC

ATCAACGCCAGCGTGGTGAACATCCAGAAGGAGATCGATCGGCT

GAACGAGGTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTG

CAGGAGCTGGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGT

ACATCTGGCTGGGCTTCATCGCCGGCCTGATCGCCATCGTGATG

GTGACCATCATGCTGTGCTGCATGACCAGCTGCTGCAGCTGCCT

GAAGGGCTGTTGCAGCTGCGGCAGCTGCTGCAAGTTCGACGAG

GACGACAGCGAGCCCGTGCTGAAGGGCGTGAAGCTGCACTACA

CCTGATAATAGGCTGGAGCCTCGGTGGCCTAGCTTCTTGCCCCT

TGGGCCTCCCCCCAGCCCCTCCTCCCCTTCCTGCACCCGTACCC

CCGTGGTCTTTGAATAAAGTCTGAGTGGGCGGCAAAAAAAAA

Spike
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTG
143

glycoprotein
CGTGAACCTGACCACCCGGACCCAGCTGCCACCAGCCTACACC

with one
AACAGCTTCACCCGGGGCGTCTACTACCCCGACAAGGTGTTCCG

stabilizing
GAGCAGCGTCCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCT

mutations
TCAGCAACGTGACCTGGTTCCACGCCATCCACGTGAGCGGCAC

CAACGGCACCAAGCGGTTCGACAACCCCGTGCTGCCCTTCAAC

GACGGCGTGTACTTCGCCAGCACCGAGAAGAGCAACATCATCC

GGGGCTGGATCTTCGGCACCACCCTGGACAGCAAGACCCAGAG

CCTGCTGATCGTGAATAACGCCACCAACGTGGTGATCAAGGTGT

GCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTACTAC

CACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCCGGGTGT

ACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCC

CTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACC

TGCGGGAGTTCGTGTTCAAGAACATCGACGGCTACTTCAAGATC

TACAGCAAGCACACCCCAATCAACCTGGTGCGGGATCTGCCCCA

GGGCTTCTCAGCCCTGGAGCCCCTGGTGGACCTGCCCATCGGC

ATCAACATCACCCGGTTCCAGACCCTGCTGGCCCTGCACCGGAG

CTACCTGACCCCAGGCGACAGCAGCAGCGGGTGGACAGCAGGC

GCGGCTGCTTACTACGTGGGCTACCTGCAGCCCCGGACCTTCCT

GCTGAAGTACAACGAGAACGGCACCATCACCGACGCCGTGGAC

TGCGCCCTGGACCCTCTGAGCGAGACCAAGTGCACCCTGAAGA

GCTTCACCGTGGAGAAGGGCATCTACCAGACCAGCAACTTCCGG

GTGCAGCCCACCGAGAGCATCGTGCGGTTCCCCAACATCACCAA

CCTGTGCCCCTTCGGCGAGGTGTTCAACGCCACCCGGTTCGCC

AGCGTGTACGCCTGGAACCGGAAGCGGATCAGCAACTGCGTGG

CCGACTACAGCGTGCTGTACAACAGCGCCAGCTTCAGCACCTTC

AAGTGCTACGGCGTGAGCCCCACCAAGCTGAACGACCTGTGCTT

CACCAACGTGTACGCCGACAGCTTCGTGATCCGTGGCGACGAG

GTGCGGCAGATCGCACCCGGCCAGACAGGCAAGATCGCCGACT

ACAACTACAAGCTGCCCGACGACTTCACCGGCTGCGTGATCGCC

TGGAACAGCAACAACCTCGACAGCAAGGTGGGCGGCAACTACA

ACTACCTGTACCGGCTGTTCCGGAAGAGCAACCTGAAGCCCTTC

GAGCGGGACATCAGCACCGAGATCTACCAAGCCGGCTCCACCC

CTTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCTCTGCAG

AGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCT

ACCGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCAGC

CACCGTGTGTGGCCCCAAGAAGAGCACCAACCTGGTGAAGAAC

AAGTGCGTGAACTTCAACTTCAACGGCCTTACCGGCACCGGCGT

GCTGACCGAGAGCAACAAGAAATTCCTGCCCTTTCAGCAGTTCG

GCCGGGACATCGCCGACACCACCGACGCTGTGCGGGATCCCCA

GACCCTGGAGATCCTGGACATCACCCCTTGCAGCTTCGGCGGC

GTGAGCGTGATCACCCCAGGCACCAACACCAGCAACCAGGTGG

CCGTGCTGTACCAGGACGTGAACTGCACCGAGGTGCCCGTGGC

CATCCACGCCGACCAGCTGACACCCACCTGGCGGGTCTACAGC

ACCGGCAGCAACGTGTTCCAGACCCGGGCCGGTTGCCTGATCG

GCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCAT

CGGCGCCGGCATCTGTGCCAGCTACCAGACCCAGACCAATTCA

CCCGGCAGCGCCAGCAGCGTGGCCAGCCAGAGCATCATCGCCT

ACACCATGAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAA

CAACAGCATCGCCATCCCCACCAACTTCACCATCAGCGTGACCA

CCGAGATTCTGCCCGTGAGCATGACCAAGACCAGCGTGGACTG

CACCATGTACATCTGCGGCGACAGCACCGAGTGCAGCAACCTG

CTGCTGCAGTACGGCAGCTTCTGCACCCAGCTGAACCGGGCCC

TGACCGGCATCGCCGTGGAGCAGGACAAGAACACCCAGGAGGT

GTTCGCCCAGGTGAAGCAGATCTACAAGACCCCTCCCATCAAGG

ACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCCCGACCCCAGC

AAGCCCAGCAAGCGGAGCCCCATCGAGGACCTGCTGTTCAACA

AGGTGACCCTAGCCGACGCCGGCTTCATCAAGCAGTACGGCGA

CTGCCTCGGCGACATAGCCGCCCGGGACCTGATCTGCGCCCAG

AAGTTCAACGGCCTGACCGTGCTGCCTCCCCTGCTGACCGACGA

GATGATCGCCCAGTACACCAGCGCCCTGTTAGCCGGAACCATCA

CCAGCGGCTGGACTTTCGGCGCTGGCCCCGCTCTGCAGATCCC

CTTCCCCATGCAGATGGCCTACCGGTTCAACGGCATCGGCGTGA

CCCAGAACGTGCTGTACGAGAACCAGAAGCTGATCGCCAACCAG

TTCAACAGCGCCATCGGCAAGATCCAGGACAGCCTGAGCAGCA

CCCCTAGCGCCCTGGGCAAGCTGCAGGACGTGGTGAACCAGAA

CGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAGCAGCAAC

TTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCCGGC

TGGACCCTCCCGAGGCCGAGGTGCAGATCGACCGGCTGATCAC

TGGCCGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTG

ATCCGGGCCGCCGAGATTCGGGCCAGCGCCAACCTGGCCGCCA

CCAAGATGAGCGAGTGCGTGCTGGGCCAGAGCAAGCGGGTGGA

CTTCTGCGGCAAGGGCTACCACCTGATGAGCTTTCCCCAGAGCG

CACCCCACGGAGTGGTGTTCCTGCACGTGACCTACGTGCCCGC

CCAGGAGAAGAACTTCACCACCGCCCCAGCCATCTGCCACGAC

GGCAAGGCCCACTTTCCCCGGGAGGGCGTGTTCGTGAGCAACG

GCACCCACTGGTTCGTGACCCAGCGGAACTTCTACGAGCCCCA

GATCATCACCACCGACAACACCTTCGTGAGCGGCAACTGCGACG

TGGTGATCGGCATCGTGAACAACACCGTGTACGATCCCCTGCAG

CCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAATCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGC

ATCAACGCCAGCGTGGTGAACATCCAGAAGGAGATCGATCGGCT

GAACGAGGTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTG

CAGGAGCTGGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGT

ACATCTGGCTGGGCTTCATCGCCGGCCTGATCGCCATCGTGATG

GTGACCATCATGCTGTGCTGCATGACCAGCTGCTGCAGCTGCCT

GAAGGGCTGTTGCAGCTGCGGCAGCTGCTGCAAGTTCGACGAG

GACGACAGCGAGCCCGTGCTGAAGGGCGTGAAGCTGCACTACA

CCTGATAATAGGCTGGAGCCTCGGTGGCCTAGCTTCTTGCCCCT

TGGGCCTCCCCCCAGCCCCTCCTCCCCTTCCTGCACCCGTACCC

CCGTGGTCTTTGAATAAAGTCTGAGTGGGCGGCAAAAAAAAA

Nucleocapsid
ATGAGCGACAACGGCCCCCAGAACCAGAGGAACGCCCCCAGGA
150

TCACCTTCGGCGGCCCCAGCGACAGCACCGGCAGCAACCAGAA

CGGCGAGAGGAGCGGCGCCAGGAGCAAGCAGAGGAGGCCCCA

GGGCCTGCCCAACAACACCGCCAGCTGGTTCACCGCCCTGACC

CAGCACGGCAAGGAGGACCTGAAGTTCCCCAGGGGCCAGGGC

GTGCCCATCAACACCAACAGCAGCCCCGACGACCAGATCGGCT

ACTACAGGAGGGCCACCAGGAGGATCAGGGGCGGCGACGGCA

AGATGAAGGACCTGAGCCCCAGGTGGTACTTCTACTACCTGGGC

ACCGGCCCCGAGGCCGGCCTGCCCTACGGCGCCAACAAGGAC

GGCATCATCTGGGTGGCCACCGAGGGCGCCCTGAACACCCCCA

AGGACCACATCGGCACCAGGAACCCCGCCAACAACGCCGCCAT

CGTGCTGCAGCTGCCCCAGGGCACCACCCTGCCCAAGGGCTTC

TACGCCGAGGGCAGCAGGGGCGGCAGCCAGGCCAGCAGCAGG

AGCAGCAGCAGGAGCAGGAACAGCAGCAGGAACAGCACCCCCG

GCAGCAGCAGGGGCACCAGCCCCGCCAGGATGGCCGGCAACG

GCGGCGACGCCGCCCTGGCCCTGCTGCTGCTGGACAGGCTGAA

CCAGCTGGAGAGCAAGATGAGCGGCAAGGGCCAGCAGCAGCAG

GGCCAGACCGTGACCAAGAAGAGCGCCGCCGAGGCCAGCAAGA

AGCCCAGGCAGAAGAGGACCGCCACCAAGGCCTACAACGTGAC

CCAGGCCTTCGGCAGGAGGGGCCCCGAGCAGACCCAGGGCAA

CTTCGGCGACCAGGAGCTGATCAGGCAGGGCACCGACTACAAG

CACTGGCCCCAGATCGCCCAGTTCGCCCCCAGCGCCAGCGCCT

TCTTCGGCATGAGCAGGATCGGCATGGAGGTGACCCCCAGCGG

CACCTGGCTGACCTACACCGGCGCCATCAAGCTGGACGACAAG

GACCCCAACTTCAAGGACCAGGTGATCCTGCTGAACAAGCACAT

CGACGCCTACAAGACCTTCCCCCCCACCGAGCCCAAGAAGGAC

AAGAAGAAGAAGGCCGACGAGACCCAGGCCCTGCCCCAGAGGC

AGAAGAAGCAGCAGACCGTGACCCTGCTGCCCGCCGCCGACCT

GGACGACTTCAGCAAGCAGCTGCAGCAGAGCATGAGCAGCGCC

GACAGCACCCAGGCC

ORFtab (non-
CAAACCACTGAAACAGCWCACTCTTGTAATGTTAACCGCTTTAAT
151

annotated)
GTGGCTATTACAAGAGCAAAAATTGGCATTGTGCATAATGTCT

GACAGAGATCTTTATGACAAGCTGCAATTCACAAGTCTAGAAGTA

CCGCGTCGTAACGTGGCTACATTACAAGCGGAAAATGTAACTGG

ACTCTTTAAGGACTGTAGTAAGATCATAACTGGTCTTCATCCTAC

ACAAGCACCTACACACCTTAGTGTTGATACAAAATTCAAGACTGA

GGGACTATGTGTTGACATACCAGGCATWCCWAAGGACATGACC

TATMGWAGACTCATCTCYATGATGGGTTTCAAAATGAATTAYCAA

GTTAATGGTTACCCTAAYATGTTYATCACCCGYGARGAAGCCATM

MGMCAYGTWCGTGCATGGATTGGCTTTGATGTAGAGGGKTGTC

ATGCTACTAGGGATGCTGTCGGTACTAACCTACCTCTCCAGTTAG

GATTTTCTACAGGTGTTAACTTAGTAGCTGTACCAACTGGCTATG

TTGACACTGAAAACAATACAGAATTCACCAGAGTTAATGCAAAAC

CTCCACCAGGTGACCAATTTAAACATCTTATACCACTTATGTACA

AAGGTTTACCCTGGAACATAGTGCGTATCAAGATAGTACAAATGC

TCAGTGATACACTGAAAGGATTATCRGACAGAGTTGTGTTTGTCC

TATGGGCACATGGCTTTGAACTTACATCAATGAAGTACTTTGTCA

AGATTGGACCTGAAAGAACGTGTTGTCTGTGTGACAAACGTGCA

ACTTGTTTTTCTACTTCATCAGACAATTATGCCTGCTGGAACCATT

CTGTGGGTTTTGACTATGTCTATAATCCATTTATGATTGATGTCCA

GCAGTGGGGTTTTACAGGTAACCTTCAGAGTAATCACGATCAGC

ATTGCCAAGTGCATGGCAACGCTCATGTGGCTAGTTGTGATGCT

ATCATGACTAGATGTTTAGCAGTCCATGAGTGCTTTGTTAAGCGC

GTTGACTGGTCTGTTGAGTACCCAATTATAGGTGATGAACTGAAG

ATCAATGCCGCATGCAGAAAAGTGCAACATATGGTTGTAAAGTCT

GCATTGCTTGCTGACAAATTCCCAGTTCTTCATGACATTGGAAAC

CCAAAGGCTATCAAATGTGTCCCRCAGGCTGAAGTGGATTGGAA

GTTCTATGATGCTCAGCCCTGCAGTGACAAAGCTTATAAAATAAA

AGAACTCTTCTATTCTTATGCTACACATCATGATAAATTCATTGAT

GGTGTTTGTTTATTTTGGAATTGTAACGTTGATCGTTACCCTGCC

AATGCTATTGTRTGCAGGTTCGACACGAGAGTCTTGTCAAATTTG

AACTTGCCAGGTTGTGATGGTGGTAGTTTGTATGTAAATAAGCAT

GCATTCCACACTCCAGCTTTTGATAAAAGTGCATTTACTAATTTAA

AGCAATTGCCTTTCTTTTATTACTCTGACAGTCCCTGTGAGTCAC

ATGGCAAGCAGGTTGTTTCTGACATTGATTATGTACCACTCAAAT

CTGCTACRTGTATAACACGATGCAATTTGGGRGGTGCTGTTTGC

AGACATCATGCAAATGAGTACCGACAGTACTTGGATGCATACAAT

ATGATGATTTCTGCTGGCTTTAGCCTCTGGATTTACAAACAGTTT

GACACTTATAACCTGTGGAACACCTTTACCAGGTTACAGAGTTTA

GAAAATGTGGCTTACAATGTTGTTAACAAAGGACACTTCGATGGA

CAAGCTGGTGAAGCACCTGTTTCCGTCATTAATAATGTTGTTTAC

ACAAAGGTAGATGGTGTTGATGTAGAGATCTTTGAAAACAAGACA

ACACTTCCTGTTAATGTTGCATTTGAGCTTTGGGCTAAGCGTAAC

ATTAAACCAGTGCCAGAGATTAAGATACTCAATAATTTGGGTGTC

GATATCGCTGCTAATACTGTAATCTGGGACTACAAGAGAGAAGC

ACCAGCACATATGTCAACAATAGGTGTCTGCACAATGACTGACAT

TGCCAAGAAACCTACTGAGAGTGCTTGTTCCTCGCTTACTGTCTT

ATTTGATGGTAGAGTGGAAGGACAGGTAGACCTTTTTAGAAATGC

CCGTAATGGTGTTTTAATAACAGAAGGTTCAGTTAAAGGTTTAAT

ACCTTCAAAGGGACCAGCACAAGCTAGTGTCAATGGAGTCACAT

TAATTGGAGAATCAGTAAAAACACAGTTTAATTATTTTAAGAAAGT

AGATGGCATCATTCAACAGTTGCCTGAAACCTACTTTACTCAGAG

CCGAGACTTAGAGGATTTCAAGCCCAGATCACAAATGGAAACTG

ACTTTCTTGAGCTCGCTATGGATGAATTCATACAACGGTACAAGC

TTGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATAGTC

ATGGACAGCTTGGTGGACTTCATCTAATGATTGGTCTAGCTAAGC

GCTCACAAGATTCACCACTTAAATTAGAGGATTTTATCCCTACGG

ACAGTACAGTGAAAAATTATTTCATAACAGATGCGCAAACAGGTT

CATCAAAATGCGTGTGCTCTGTTATTGATCTTCTGCTTGATGACT

TTGTTGAGATAATAAAGTCACAAGATTTATCAGTGGTTTCAAAGG

TGGTCAAAGTCACAATTGACTATGCTGAAATTTCATTCATGTTATG

GTGTAAGGATGGACATGTTGAAACCTTACCCAAAATTACAAGC

GAGTCAGGCGTGGCAACCAGGAGTTGCAATGCCTAACTTGTATA

AGATGCAGAGAATGCTTCTTGAAAAATGTGACCTTCAGAATTATG

GTGAAAATGCTGTCATACCAAARGGAATAATGATGAATGTCGCAA

AATATACTCAACTGTGTCAATATTTAAATACACTYACATTAGCYGT

GCCATATAATATGAGAGTTATCCAMTTGGTGCTGGCTCRGACAA

AGGAGTTGCACCCGGCACAGCTGTTCTCAGACAGTGGTTGCCAA

TTGGCACACTACTTGTTGATTCAGATCTTAACGACTTCGTCTCTG

ACGCTGATTCCACTCTAATTGGAGACTGTGCAACCGTACATACAG

CTAACAAATGGGATCTCATTATTAGCGATATGTATGATCCTAAAA

CCAAACACGTGACAAAGGAAAATGATTCAAAAGAAGGATTTTTCA

CTTACCTGTGTGGATTTATTAAACAAAAATTAGCCCTGGGAGGCT

CTGTGGCTGTAAAGATAACTGAGCATTCTTGGAATGCGGATCTCT

ACAAGCTCATGGGACATTTCTCATGGTGGACAGCTTTTGTTACAA

ATGTTAATGCATCTTCATCAGAAGCATTTTTAATTGGAGTTAACTA

TCTTGGTAAGCCAAAAGAACAAATTGATGGTTACACCATGCATGC

TAACTACATTCTGGAGGAATACAAACCCGATTCAATTGTCTTC

CTATTCACTTTTTGACATGAGTAAGTTCCCTCTTAAATTAAGGGGA

ACAGCTGTCATGTCTTTAAAGGAGAACCAAATCAATGAAATGATT

TATTCTCTACTTGAAAAAGGCAGACTTATCATTAGGGAAAACAAC

AGAGTTGTTGTCTCAAGTGATGTTCTTGTTAATAACTAAACGAAC

A

Orfta/b
GACCAGGAACCCCGCCTGGAGGAAGGCCGTGTTCATCAGCCCC
348

TACAACAGCCAGAACGCCGTGGCCAGCAAGATCCTGGGCCTGC

CCACCCAGACCGTGGACAGCAGCCAGGGCAGCGAGTACGACTA

CGTGATCTTCACCCAGACCACCGAGACCGCCCACAGCTGCAAC

GTGAACAGGTTCAACGTGGCCATCACCAGGGCCAAGGTGGGCA

TCCTGTGCATCATGAGCGACAGGGACCTGTACGACAAGCTGCAG

TTCACCAGCCTGGAGATCCCCAGGAGGAACGTGGCCACCCTGC

AGGCCGAGAACGTGACCGGCCTGTTCAAGGACTGCAGCAAGGT

GATCACCGGCCTGCACCCCACCCAGGCCCCCACCCACCTGAGC

GTGGACACCAAGTTCAAGACCGAGGGCCTGTGCGTGGACATCC

CCGGCATCCCCAAGGACATGACCTACAGGAGGCTGATCAGCAT

GATGGGCTTCAAGATGAACTACCAGGTGAACGGCTACCCCAACA

TGTTCATCACCAGGGAGGAGGCCATCAGGCACGTGAGGGCCTG

GATCGGCTTCGACGTGGAGGGCTGCCACGCCACCAGGGAGGCC

GTGGGCACCAACCTGCCCCTGCAGCTGGGCTTCAGCACCGGCG

TGAACCTGGTGGCCGTGCCCACCGGCTACGTGGACACCCCCAA

CAACACCGACTTCAGCAGGGTGAGCGCCAAGCCCCCCCCCGGC

GACCAGTTCAAGCACCTGATCCCCCTGATGTACAAGGGCCTGCC

CTGGAACGTGGTGAGGATCAAGATCGTGCAGATGCTGAGCGAC

ACCCTGAAGAACCTGAGCGACAGGGTGGTGTTCGTGCTGTGGG

CCCACGGCTTCGAGCTGACCAGCATGAAGTACTTCGTGAAGATC

GGCCCCGAGAGGACCTGCTGCCTGTGCGACAGGAGGGCCACCT

GCTTCAGCACCGCCAGCGACACCTACGCCTGCTGGCACCACAG

CATCGGCTTCGACTACGTGTACAACCCCTTCATGATCGACGTGC

AGCAGTGGGGCTTCACCGGCAACCTGCAGAGCAACCACGACCT

GTACTGCCAGGTGCACGGCAACGCCCACGTGGCCAGCTGCGAC

GCCATCATGACCAGGTGCCTGGCCGTGCACGAGTGCTTCGTGA

AGAGGGTGGACTGGACCATCGAGTACCCCATCATCGGCGACGA

GCTGAAGATCAACGCCGCCTGCAGGAAGGTGCAGCACATGGTG

GTGAAGGCCGCCCTGCTGGCCGACAAGTTCCCCGTGCTGCACG

ACATCGGCAACCCCAAGGCCATCAAGTGCGTGCCCCAGGCCGA

CGTGGAGTGGAAGTTCTACGACGCCCAGCCCTGCAGCGACAAG

GCCTACAAGATCGAGGAGCTGTTCTACAGCTACGCCACCCACAG

CGACAAGTTCACCGACGGCGTGTGCCTGTTCTGGAACTGCAACG

TGGACAGGTACCCCGCCAACAGCATCGTGTGCAGGTTCGACAC

CAGGGTGCTGAGCAACCTGAACCTGCCCGGCTGCGACGGCGGC

AGCCTGTACGTGAACAAGCACGCCTTCCACACCCCCGCCTTCGA

CAAGAGCGCCTTCGTGAACCTGAAGCAGCTGCCCTTCTTCTACT

ACAGCGACAGCCCCTGCGAGAGCCACGGCAAGCAGGTGGTGAG

CGACATCGACTACGTGCCCCTGAAGAGCGCCACCTGCATCACCA

GGTGCAACCTGGGCGGCGCCGTGTGCAGGCACCACGCCAACGA

GTACAGGCTGTACCTGGACGCCTACAACATGATGATCAGCGCCG

GCTTCAGCCTGTGGGTGTACAAGCAGTTCGACACCTACAACCTG

TGGAACACCTTCACCAGGCTGCAGAGCCTGGAGAACGTGGCCTT

CAACGTGGTGAACAAGGGCCACTTCGACGGCCAGCAGGGCGAG

GTGCCCGTGAGCATCATCAACAACACCGTGTACACCAAGGTGGA

CGGCGTGGACGTGGAGCTGTTCGAGAACAAGACCACCCTGCCC

GTGAACGTGGCCTTCGAGCTGTGGGCCAAGAGGAACATCAAGC

CCGTGCCCGAGGTGAAGATCCTGAACAACCTGGGCGTGGACAT

CGCCGCCAACACCGTGATCTGGGACTACAAGAGGGACGCCCCC

GCCCACATCAGCACCATCGGCGTGTGCAGCATGACCGACATCG

CCAAGAAGCCCACCGAGACCATCTGCGCCCCCCTGACCGTGTT

CTTCGACGGCAGGGTGGACGGCCAGGTGGACCTGTTCAGGAAC

GCCAGGAACGGCGTGCTGATCACCGAGGGCAGCGTGAAGGGC

CTGCAGCCCAGCGTGGGCCCCAAGCAGGCCAGCCTGAACGGC

GTGACCCTGATCGGCGAGGCCGTGAAGACCCAGTTCAACTACTA

CAAGAAGGTGGACGGCGTGGTGCAGCAGCTGCCCGAGACCTAC

TTCACCCAGAGCAGGAACCTGCAGGAGTTCAAGCCCAGGAGCC

AGATGGAGATCGACTTCCTGGAGCTGGCCATGGACGAGTTCATC

GAGAGGTACAAGCTGGAGGGCTACGCCTTCGAGCACATCGTGT

ACGGCGACTTCAGCCACAGCCAGCTGGGCGGCCTGCACCTGCT

GATCGGCCTGGCCAAGAGGTTCAAGGAGAGCCCCTTCGAGCTG

GAGGACTTCATCCCCATGGACAGCACCGTGAAGAACTACTTCAT

CACCGACGCCCAGACCGGCAGCAGCAAGTGCGTGTGCAGCGTG

ATCGACCTGCTGCTGGACGACTTCGTGGAGATCATCAAGAGCCA

GGACCTGAGCGTGGTGAGCAAGGTGGTGAAGGTGACCATCGAC

TACACCGAGATCAGCTTCATGCTGTGGTGCAAGGACGGCCACGT

GGAGACCTTCTACCCCAAGCTGCAGAGCAGCCAGGCCTGGCAG

CCCGGCGTGGCCATGCCCAACCTGTACAAGATGCAGAGGATGC

TGCTGGAGAAGTGCGACCTGCAGAACTACGGCGACAGCGCCAC

CCTGCCCAAGGGCATCATGATGAACGTGGCCAAGTACACCCAGC

TGTGCCAGTACCTGAACACCCTGACCCTGGCCGTGCCCTACAAC

ATGAGGGTGATCCACTTCGGCGCCGGCAGCGACAAGGGCGTGG

CCCCCGGCACCGCCGTGCTGAGGCAGTGGCTGCCCACCGGCA

CCCTGCTGGTGGACAGCGACCTGAACGACTTCGTGAGCGACGC

CGACAGCACCCTGATCGGCGACTGCGCCACCGTGCACACCGCC

AACAAGTGGGACCTGATCATCAGCGACATGTACGACCCCAAGAC

CAAGAACGTGACCAAGGAGAACGACAGCAAGGAGGGCTTCTTCA

CCTACATCTGCGGCTTCATCCAGCAGAAGCTGGCCCTGGGCGG

CAGCGTGGCCATCAAGATCACCGAGCACAGCTGGAACGCCGAC

CTGTACAAGCTGATGGGCCACTTCGCCTGGTGGACCGCCTTCGT

GACCAACGTGAACGCCAGCAGCAGCGAGGCCTTCCTGATCGGC

TGCAACTACCTGGGCAAGCCCAGGGAGCAGATCGACGGCTACG

TGATGCACGCCAACTACATCTTCTGGAGGAACACCAACCCCATC

CAGCTGAGCAGCTACAGCCTGTTCGACATGAGCAAGTTCCCCCT

GAAGCT

ORF3a
ATGGACCTGTTCATGAGGATCTTCACCATCGGCACCGTGACCCT
152

GAAGCAGGGCGAGATCAAGGACGCCACCCCCAGCGACTTCGTG

AGGGCCACCGCCACCATCCCCATCCAGGCCAGCCTGCCCTTCG

GCTGGCTGATCGTGGGCGTGGCCCTGCTGGCCGTGTTCCAGAG

CGCCAGCAAGATCATCACCCTGAAGAAGAGGTGGCAGCTGGCC

CTGAGCAAGGGCGTGCACTTCGTGTGCAACCTGCTGCTGCTGTT

CGTGACCGTGTACAGCCACCTGCTGCTGGTGGCCGCCGGCCTG

GAGGCCCCCTTCCTGTACCTGTACGCCCTGGTGTACTTCCTGCA

GAGCATCAACTTCGTGAGGATCATCATGAGGCTGTGGCTGTGCT

GGAAGTGCAGGAGCAAGAACCCCCTGCTGTACGACGCCAACTA

CTTCCTGTGCTGGCACACCAACTGCTACGACTACTGCATCCCCT

ACAACAGCGTGACCAGCAGCATCGTGATCACCAGCGGCGACGG

CACCACCAGCCCCATCAGCGAGCACGACTACCAGATCGGCGGC

TACACCGAGAAGTGGGAGAGCGGCGTGAAGGACTGCGTGGTGC

TGCACAGCTACTTCACCAGCGACTACTACCAGCTGTACAGCACC

CAGCTGAGCACCGACACCGGCGTGGAGCACGTGACCTTCTTCAT

CTACAACAAGATCGTGGACGAGCCCGAGGAGCACGTGCAGATC

CACACCATCGACGGCAGCAGCGGCGTGGTGAACCCCGTGATGG

AGCCCATCTACGACGAGCCCACCACCACCACCAGCGTGCCCCT

G

Envelope(E)
ATGTACAGCTTCGTGAGCGAGGAGACCGGCACCCTGATCGTGAA
153

CAGCGTGCTGCTGTTCCTGGCCTTCGTGGTGTTCCTGCTGGTGA

CCCTGGCCATCCTGACCGCCCTGAGGCTGTGCGCCTACTGCTG

CAACATCGTGAACGTGAGCCTGGTGAAGCCCAGCTTCTACGTGT

ACAGCAGGGTGAAGAACCTGAACAGCAGCAGGGTGCCCGACCT

GCTGGTG

Membrane(M)
ATGGCCGACAGCAACGGCACCATCACCGTGGAGGAGCTGAAGA
154

AGCTGCTGGAGCAGTGGAACCTGGTGATCGGCTTCCTGTTCCTG

ACCTGGATCTGCCTGCTGCAGTTCGCCTACGCCAACAGGAACAG

GTTCCTGTACATCATCAAGCTGATCTTCCTGTGGCTGCTGTGGC

CCGTGACCCTGGCCTGCTTCGTGCTGGCCGCCGTGTACAGGAT

CAACTGGATCACCGGCGGCATCGCCATCGCCATGGCCTGCCTG

GTGGGCCTGATGTGGCTGAGCTACTTCATCGCCAGCTTCAGGCT

GTTCGCCAGGACCAGGAGCATGTGGAGCTTCAACCCCGAGACC

AACATCCTGCTGAACGTGCCCCTGCACGGCACCATCCTGACCAG

GCCCCTGCTGGAGAGCGAGCTGGTGATCGGCGCCGTGATCCTG

AGGGGCCACCTGAGGATCGCCGGCCACCACCTGGGCAGGTGC

GACATCAAGGACCTGCCCAAGGAGATCACCGTGGCCACCAGCA

GGACCCTGAGCTACTACAAGCTGGGCGCCAGCCAGAGGGTGGC

CGGCGACAGCGGCTTCGCCGCCTACAGCAGGTACAGGATCGGC

AACTACAAGCTGAACACCGACCACAGCAGCAGCAGCGACAACAT

CGCCCTGCTGGTGCAG

ORFS
ATGTTCCACCTGGTGGACTTCCAGGTGACCATCGCCGAGATCCT
155

GCTGATCATCATGAGGACCTTCAAGGTGAGCATCTGGAACCTGG

ACTACATCATCAACCTGATCATCAAGAACCTGAGCAAGAGCCTGA

CCGAGAACAAGTACAGCCAGCTGGACGAGGAGCAGCCCATGGA

GATCGAC

ORF7a
ATGAAGATCATCCTGTTCCTGGCCCTGATCACCCTGGCCACCTG
156

CGAGCTGTACCACTACCAGGAGTGCGTGAGGGGCACCACCGTG

NSP1
ATGGAGAGCCTGGTGCCCGGCTTCAACGAGAAGACCCACGTGC
349

AGCTGAGCCTGCCCGTGCTGCAGGTGAGGGACGTGCTGGTGAG

GGGCTTCGGCGACAGCGTGGAGGAGGTGCTGAGCGAGGCCAG

GCAGCACCTGAAGGACGGCACCTGCGGCCTGGTGGAGGTGGA

GAAGGGCGTGCTGCCCCAGCTGGAGCAGCCCTACGTGTTCATC

AAGAGGAGCGACGCCAGGACCGCCCCCCACGGCCACGTGATG

GTGGAGCTGGTGGCCGAGCTGGAGGGCATCCAGTACGGCAGGA

GCGGCGAGACCCTGGGCGTGCTGGTGCCCCACGTGGGCGAGA

TCCCCGTGGCCTACAGGAAGGTGCTGCTGAGGAAGAACGGCAA

CAAGGGCGCCGGCGGCCACAGCTACGGCGCCGACCTGAAGAG

CTTCGACCTGGGCGACGAGCTGGGCACCGACCCCTACGAGGAC

TTCCAGGAGAACTGGAACACCAAGCACAGCAGCGGCGTGACCA

GGGAGCTGATGAGGGAGCTGAACGGCGGC

NSP2
GCCTACACCAGGTACGTGGACAACAACTTCTGCGGCCCCGACG
350

GCTACCCCCTGGAGTGCATCAAGGACCTGCTGGCCAGGGCCGG

CAAGGCCAGCTGCACCCTGAGCGAGCAGCTGGACTTCATCGAC

ACCAAGAGGGGCGTGTACTGCTGCAGGGAGCACGAGCACGAGA

TCGCCTGGTACACCGAGAGGAGCGAGAAGAGCTACGAGCTGCA

GACCCCCTTCGAGATCAAGCTGGCCAAGAAGTTCGACACCTTCA

ACGGCGAGTGCCCCAACTTCGTGTTCCCCCTGAACAGCATCATC

AAGACCATCCAGCCCAGGGTGGAGAAGAAGAAGCTGGACGGCT

TCATGGGCAGGATCAGGAGCGTGTACCCCGTGGCCAGCCCCAA

CGAGTGCAACCAGATGTGCCTGAGCACCCTGATGAAGTGCGAC

CACTGCGGCGAGACCAGCTGGCAGACCGGCGACTTCGTGAAGG

CCACCTGCGAGTTCTGCGGCACCGAGAACCTGACCAAGGAGGG

CGCCACCACCTGCGGCTACCTGCCCCAGAACGCCGTGGTGAAG

ATCTACTGCCCCGCCTGCCACAACAGCGAGGTGGGCCCCGAGC

ACAGCCTGGCCGAGTACCACAACGAGAGCGGCCTGAAGACCAT

CCTGAGGAAGGGCGGCAGGACCATCGCCTTCGGCGGCTGCGTG

TTCAGCTACGTGGGCTGCCACAACAAGTGCGCCTACTGGGTGCC

CAGGGCCAGCGCCAACATCGGCTGCAACCACACCGGCGTGGTG

GGCGAGGGCAGCGAGGGCCTGAACGACAACCTGCTGGAGATCC

TGCAGAAGGAGAAGGTGAACATCAACATCGTGGGCGACTTCAAG

CTGAACGAGGAGATCGCCATCATCCTGGCCAGCTTCAGCGCCA

GCACCAGCGCCTTCGTGGAGACCGTGAAGGGCCTGGACTACAA

GGCCTTCAAGCAGATCGTGGAGAGCTGCGGCAACTTCAAGGTG

ACCAAGGGCAAGGCCAAGAAGGGCGCCTGGAACATCGGCGAGC

AGAAGAGCATCCTGAGCCCCCTGTACGCCTTCGCCAGCGAGGC

CGCCAGGGTGGTGAGGAGCATCTTCAGCAGGACCCTGGAGACC

GCCCAGAACAGCGTGAGGGTGCTGCAGAAGGCCGCCATCACCA

TCCTGGACGGCATCAGCCAGTACAGCCTGAGGCTGATCGACGC

CATGATGTTCACCAGCGACCTGGCCACCAACAACCTGGTGGTGA

TGGCCTACATCACCGGCGGCGTGGTGCAGCTGACCAGCCAGTG

GCTGACCAACATCTTCGGCACCGTGTACGAGAAGCTGAAGCCCG

TGCTGGACTGGCTGGAGGAGAAGTTCAAGGAGGGCGTGGAGTT

CCTGAGGGACGGCTGGGAGATCGTGAAGTTCATCAGCACCTGC

GCCTGCGAGATCGTGGGCGGCCAGATCGTGACCTGCGCCAAGG

AGATCAAGGAGAGCGTGCAGACCTTCTTCAAGCTGGTGAACAAG

TTCCTGGCCCTGTGCGCCGACAGCATCATCATCGGCGGCGCCA

AGCTGAAGGCCCTGAACCTGGGCGAGACCTTCGTGACCCACAG

CAAGGGCCTGTACAGGAAGTGCGTGAAGAGCAGGGAGGAGACC

GGCCTGCTGATGCCCCTGAAGGCCCCCAAGGAGATCATCTTCCT

GGAGGGCGAGACCCTGCCCACCGAGGTGCTGACCGAGGAGGT

GGTGCTGAAGACCGGCGACCTGCAGCCCCTGGAGCAGCCCACC

AGCGAGGCCGTGGAGGCCCCCCTGGTGGGCACCCCCGTGTGC

ATCAACGGCCTGATGCTGCTGGAGATCAAGGACACCGAGAAGTA

CTGCGCCCTGGCCCCCAACATGATGGTGACCAACAACACCTTCA

CCCTGAAGGGCGGC

Nsp3
GCCCCCACCAAGGTGACCTTCGGCGACGACACCGTGATCGAGG
157

TGCAGGGCTACAAGAGCGTGAACATCACCTTCGAGCTGGACGA

GAGGATCGACAAGGTGCTGAACGAGAAGTGCAGCGCCTACACC

GTGGAGCTGGGCACCGAGGTGAACGAGTTCGCCTGCGTGGTGG

CCGACGCCGTGATCAAGACCCTGCAGCCCGTGAGCGAGCTGCT

GACCCCCCTGGGCATCGACCTGGACGAGTGGAGCATGGCCACC

TACTACCTGTTCGACGAGAGCGGCGAGTTCAAGCTGGCCAGCCA

CATGTACTGCAGCTTCTACCCCCCCGACGAGGACGAGGAGGAG

GGCGACTGCGAGGAGGAGGAGTTCGAGCCCAGCACCCAGTACG

AGTACGGCACCGAGGACGACTACCAGGGCAAGCCCCTGGAGTT

CGGCGCCACCAGCGCCGCCCTGCAGCCCGAGGAGGAGCAGGA

GGAGGACTGGCTGGACGACGACAGCCAGCAGACCGTGGGCCA

GCAGGACGGCAGCGAGGACAACCAGACCACCACCATCCAGACC

ATCGTGGAGGTGCAGCCCCAGCTGGAGATGGAGCTGACCCCCG

TGGTGCAGACCATCGAGGTGAACAGCTTCAGCGGCTACCTGAAG

CTGACCGACAACGTGTACATCAAGAACGCCGACATCGTGGAGGA

GGCCAAGAAGGTGAAGCCCACCGTGGTGGTGAACGCCGCCAAC

GTGTACCTGAAGCACGGCGGCGGCGTGGCCGGCGCCCTGAACA

AGGCCACCAACAACGCCATGCAGGTGGAGAGCGACGACTACAT

CGCCACCAACGGCCCCCTGAAGGTGGGCGGCAGCTGCGTGCTG

AGCGGCCACAACCTGGCCAAGCACTGCCTGCACGTGGTGGGCC

CCAACGTGAACAAGGGCGAGGACATCCAGCTGCTGAAGAGCGC

CTACGAGAACTTCAACCAGCACGAGGTGCTGCTGGCCCCCCTG

CTGAGCGCCGGCATCTTCGGCGCCGACCCCATCCACAGCCTGA

GGGTGTGCGTGGACACCGTGAGGACCAACGTGTACCTGGCCGT

GTTCGACAAGAACCTGTACGACAAGCTGGTGAGCAGCTTCCTGG

AGATGAAGAGCGAGAAGCAGGTGGAGCAGAAGATCGCCGAGAT

CCCCAAGGAGGAGGTGAAGCCCTTCATCACCGAGAGCAAGCCC

AGCGTGGAGCAGAGGAAGCAGGACGACAAGAAGATCAAGGCCT

GCGTGGAGGAGGTGACCACCACCCTGGAGGAGACCAAGTTCCT

GACCGAGAACCTGCTGCTGTACATCGACATCAACGGCAACCTGC

ACCCCGACAGCGCCACCCTGGTGAGCGACATCGACATCACCTTC

CTGAAGAAGGACGCCCCCTACATCGTGGGCGACGTGGTGCAGG

AGGGCGTGCTGACCGCCGTGGTGATCCCCACCAAGAAGGCCGG

CGGCACCACCGAGATGCTGGCCAAGGCCCTGAGGAAGGTGCCC

ACCGACAACTACATCACCACCTACCCCGGCCAGGGCCTGAACG

GCTACACCGTGGAGGAGGCCAAGACCGTGCTGAAGAAGTGCAA

GAGCGCCTTCTACATCCTGCCCAGCATCATCAGCAACGAGAAGC

AGGAGATCCTGGGCACCGTGAGCTGGAACCTGAGGGAGATGCT

GGCCCACGCCGAGGAGACCAGGAAGCTGATGCCCGTGTGCGTG

GAGACCAAGGCCATCGTGAGCACCATCCAGAGGAAGTACAAGG

GCATCAAGATCCAGGAGGGCGTGGTGGACTACGGCGCCAGGTT

CTACTTCTACACCAGCAAGACCACCGTGGCCAGCCTGATCAACA

CCCTGAACGACCTGAACGAGACCCTGGTGACCATGCCCCTGGG

CTACGTGACCCACGGCCTGAACCTGGAGGAGGCCGCCAGGTAC

ATGAGGAGCCTGAAGGTGCCCGCCACCGTGAGCGTGAGCAGCC

CCGACGCCGTGACCGCCTACAACGGCTACCTGACCAGCAGCAG

CAAGACCCCCGAGGAGCACTTCATCGAGACCATCAGCCTGGCC

GGCAGCTACAAGGACTGGAGCTACAGCGGCCAGAGCACCCAGC

TGGGCATCGAGTTCCTGAAGAGGGGCGACAAGAGCGTGTACTA

CACCAGCAACCCCACCACCTTCCACCTGGACGGCGAGGTGATC

ACCTTCGACAACCTGAAGACCCTGCTGAGCCTGAGGGAGGTGA

GGACCATCAAGGTGTTCACCACCGTGGACAACATCAACCTGCAC

Nsp5
AGCGGCTTCAGGAAGATGGCCTTCCCCAGCGGCAAGGTGGAGG
158

GCTGCATGGTGCAGGTGACCTGCGGCACCACCACCCTGAACGG

CCTGTGGCTGGACGACGTGGTGTACTGCCCCAGGCACGTGATC

TGCACCAGCGAGGACATGCTGAACCCCAACTACGAGGACCTGCT

GATCAGGAAGAGCAACCACAACTTCCTGGTGCAGGCCGGCAAC

GTGCAGCTGAGGGTGATCGGCCACAGCATGCAGAACTGCGTGC

TGAAGCTGAAGGTGGACACCGCCAACCCCAAGACCCCCAAGTA

CAAGTTCGTGAGGATCCAGCCCGGCCAGACCTTCAGCGTGCTG

GCCTGCTACAACGGCAGCCCCAGCGGCGTGTACCAGTGCGCCA

TGAGGCCCAACTTCACCATCAAGGGCAGCTTCCTGAACGGCAGC

TGCGGCAGCGTGGGCTTCAACATCGACTACGACTGCGTGAGCTT

CTGCTACATGCACCACATGGAGCTGCCCACCGGCGTGCACGCC

GGCACCGACCTGGAGGGCAACTTCTACGGCCCCTTCGTGGACA

GGCAGACCGCCCAGGCCGCCGGCACCGACACCACCATCACCGT

GAACGTGCTGGCCTGGCTGTACGCCGCCGTGATCAACGGCGAC

AGGTGGTTCCTGAACAGGTTCACCACCACCCTGAACGACTTCAA

CCTGGTGGCCATGAAGTACAACTACGAGCCCCTGACCCAGGAC

CACGTGGACATCCTGGGCCCCCTGAGCGCCCAGACCGGCATCG

CCGTGCTGGACATGTGCGCCAGCCTGAAGGAGCTGCTGCAGAA

CGGCATGAACGGCAGGACCATCCTGGGCAGCGCCCTGCTGGAG

GACGAGTTCACCCCCTTCGACGTGGTGAGGCAGTGCAGCGGCG

TGACCTTCCAG

NSP6
AGCGCCGTGAAGAGGACCATCAAGGGCACCCACCACTGGCTGC
351

TGCTGACCATCCTGACCAGCCTGCTGGTGCTGGTGCAGAGCAC

CCAGTGGAGCCTGTTCTTCTTCCTGTACGAGAACGCCTTCCTGC

CCTTCGCCATGGGCATCATCGCCATGAGCGCCTTCGCCATGATG

TTCGTGAAGCACAAGCACGCCTTCCTGTGCCTGTTCCTGCTGCC

CAGCCTGGCCACCGTGGCCTACTTCAACATGGTGTACATGCCCG

CCAGCTGGGTGATGAGGATCATGACCTGGCTGGACATGGTGGA

CACCAGCCTGAGCGGCTTCAAGCTGAAGGACTGCGTGATGTAC

GCCAGCGCCGTGGTGCTGCTGATCCTGATGACCGCCAGGACCG

TGTACGACGACGGCGCCAGGAGGGTGTGGACCCTGATGAACGT

GCTGACCCTGGTGTACAAGGTGTACTACGGCAACGCCCTGGAC

CAGGCCATCAGCATGTGGGCCCTGATCATCAGCGTGACCAGCAA

CTACAGCGGCGTGGTGACCACCGTGATGTTCCTGGCCAGGGGC

ATCGTGTTCATGTGCGTGGAGTACTGCCCCATCTTCTTCATCACC

GGCAACACCCTGCAGTGCATCATGCTGGTGTACTGCTTCCTGGG

CTACTTCTGCACCTGCTACTTCGGCCTGTTCTGCCTGCTGAACA

GGTACTTCAGGCTGACCCTGGGCGTGTACGACTACCTGGTGAG

CACCCAGGAGTTCAGGTACATGAACAGCCAGGGCCTGCTGCCC

CCCAAGAACAGCATCGACGCCTTCAAGCTGAACATCAAGCTGCT

GGGCGTGGGCGGCAAGCCCTGCATCAAGGTGGCCACCGTGCA

G

NSP7
AGCAAGATGAGCGACGTGAAGTGCACCAGCGTGGTGCTGCTGA
352

GCGTGCTGCAGCAGCTGAGGGTGGAGAGCAGCAGCAAGCTGTG

GGCCCAGTGCGTGCAGCTGCACAACGACATCCTGCTGGCCAAG

GACACCACCGAGGCCTTCGAGAAGATGGTGAGCCTGCTGAGCG

TGCTGCTGAGCATGCAGGGCGCCGTGGACATCAACAAGCTGTG

CGAGGAGATGCTGGACAACAGGGCCACCCTGCAG

NSP8
GCCATCGCCAGCGAGTTCAGCAGCCTGCCCAGCTACGCCGCCT
353

TCGCCACCGCCCAGGAGGCCTACGAGCAGGCCGTGGCCAACG

GCGACAGCGAGGTGGTGCTGAAGAAGCTGAAGAAGAGCCTGAA

CGTGGCCAAGAGCGAGTTCGACAGGGACGCCGCCATGCAGAGG

AAGCTGGAGAAGATGGCCGACCAGGCCATGACCCAGATGTACA

AGCAGGCCAGGAGCGAGGACAAGAGGGCCAAGGTGACCAGCG

CCATGCAGACCATGCTGTTCACCATGCTGAGGAAGCTGGACAAC

GACGCCCTGAACAACATCATCAACAACGCCAGGGACGGCTGCG

TGCCCCTGAACATCATCCCCCTGACCACCGCCGCCAAGCTGATG

GTGGTGATCCCCGACTACAACACCTACAAGAACACCTGCGACGG

CACCACCTTCACCTACGCCAGCGCCCTGTGGGAGATCCAGCAG

GTGGTGGACGCCGACAGCAAGATCGTGCAGCTGAGCGAGATCA

GCATGGACAACAGCCCCAACCTGGCCTGGCCCCTGATCGTGAC

CGCCCTGAGGGCCAACAGCGCCGTGAAGCTGCA

NSP9
AACAACGAGCTGAGCCCCGTGGCCCTGAGGCAGATGAGCTGCG
354

CCGCCGGCACCACCCAGACCGCCTGCACCGACGACAACGCCCT

GGCCTACTACAACACCACCAAGGGCGGCAGGTTCGTGCTGGCC

CTGCTGAGCGACCTGCAGGACCTGAAGTGGGCCAGGTTCCCCA

AGAGCGACGGCACCGGCACCATCTACACCGAGCTGGAGCCCCC

CTGCAGGTTCGTGACCGACACCCCCAAGGGCCCCAAGGTGAAG

TACCTGTACTTCATCAAGGGCCTGAACAACCTGAACAGGGGCAT

GGTGCTGGGCAGCCTGGCCGCCACCGTGAGGCTGCAG

NSP10
GCCGGCAACGCCACCGAGGTGCCCGCCAACAGCACCGTGCTGA
355

GCTTCTGCGCCTTCGCCGTGGACGCCGCCAAGGCCTACAAGGA

CTACCTGGCCAGCGGCGGCCAGCCCATCACCAACTGCGTGAAG

ATGCTGTGCACCCACACCGGCACCGGCCAGGCCATCACCGTGA

CCCCCGAGGCCAACATGGACCAGGAGAGCTTCGGCGGCGCCAG

CTGCTGCCTGTACTGCAGGTGCCACATCGACCACCCCAACCCCA

AGGGCTTCTGCGACCTGAAGGGCAAGTACGTGCAGATCCCCAC

CACCTGCGCCAACGACCCCGTGGGCTTCACCCTGAAGAACACC

GTGTGCACCGTGTGCGGCATGTGGAAGGGCTACGGCTGCAGCT

GCGACCAGCTGAGGGAGCCCATGCTGCAG

Nsp
AGCGCCGACGCCCAGAGCTTCCTGAACAGGGTGTGCGGCGTGA
159

GCGCCGCCAGGCTGACCCCCTGCGGCACCGGCACCAGCACCG

ACGTGGTGTACAGGGCCTTCGACATCTACAACGACAAGGTGGCC

GGCTTCGCCAAGTTCCTGAAGACCAACTGCTGCAGGTTCCAGGA

GAAGGACGAGGACGACAACCTGATCGACAGCTACTTCGTGGTGA

AGAGGCACACCTTCAGCAACTACCAGCACGAGGAGACCATCTAC

AACCTGCTGAAGGACTGCCCCGCCGTGGCCAAGCACGACTTCTT

CAAGTTCAGGATCGACGGCGACATGGTGCCCCACATCAGCAGG

CAGAGGCTGACCAAGTACACCATGGCCGACCTGGTGTACGCCC

TGAGGCACTTCGACGAGGGCAACTGCGACACCCTGAAGGAGAT

CCTGGTGACCTACAACTGCTGCGACGACGACTACTTCAACAAGA

AGGACTGGTACGACTTCGTGGAGAACCCCGACATCCTGAGGGT

GTACGCCAACCTGGGCGAGAGGGTGAGGCAGGCCCTGCTGAAG

ACCGTGCAGTTCTGCGACGCCATGAGGAACGCCGGCATCGTGG

GCGTGCTGACCCTGGACAACCAGGACCTGAACGGCAACTGGTA

CGACTTCGGCGACTTCATCCAGACCACCCCCGGCAGCGGCGTG

CCCGTGGTGGACAGCTACTACAGCCTGCTGATGCCCATCCTGAC

CCTGACCAGGGCCCTGACCGCCGAGAGCCACGTGGACACCGAC

CTGACCAAGCCCTACATCAAGTGGGACCTGCTGAAGTACGACTT

CACCGAGGAGAGGCTGAAGCTGTTCGACAGGTACTTCAAGTACT

GGGACCAGACCTACCACCCCAACTGCGTGAACTGCCTGGACGA

CAGGTGCATCCTGCACTGCGCCAACTTCAACGTGCTGTTCAGCA

CCGTGTTCCCCCCCACCAGCTTCGGCCCCCTGGTGAGGAAGAT

CTTCGTGGACGGCGTGCCCTTCGTGGTGAGCACCGGCTACCAC

TTCAGGGAGCTGGGCGTGGTGCACAACCAGGACGTGAACCTGC

ACAGCAGCAGGCTGAGCTTCAAGGAGCTGCTGGTGTACGCCGC

CGACCCCGCCATGCACGCCGCCAGCGGCAACCTGCTGCTGGAC

AAGAGGACCACCTGCTTCAGCGTGGCCGCCCTGACCAACAACG

TGGCCTTCCAGACCGTGAAGCCCGGCAACTTCAACAAGGACTTC

TACGACTTCGCCGTGAGCAAGGGCTTCTTCAAGGAGGGCAGCA

GCGTGGAGCTGAAGCACTTCTTCTTCGCCCAGGACGGCAACGC

CGCCATCAGCGACTACGACTACTACAGGTACAACCTGCCCACCA

TGTGCGACATCAGGCAGCTGCTGTTCGTGGTGGAGGTGGTGGA

CAAGTACTTCGACTGCTACGACGGCGGCTGCATCAACGCCAACC

AGGTGATCGTGAACAACCTGGACAAGAGCGCCGGCTTCCCCTTC

AACAAGTGGGGCAAGGCCAGGCTGTACTACGACAGCATGAGCT

ACGAGGACCAGGACGCCCTGTTCGCCTACACCAAGAGGAACGT

GATCCCCACCATCACCCAGATGAACCTGAAGTACGCCATCAGCG

CCAAGAACAGGGCCAGGACCGTGGCCGGCGTGAGCATCTGCAG

CACCATGACCAACAGGCAGTTCCACCAGAAGCTGCTGAAGAGCA

TCGCCGCCACCAGGGGCGCCACCGTGGTGATCGGCACCAGCAA

GTTCTACGGCGGCTGGCACAACATGCTGAAGACCGTGTACAGC

GACGTGGAGAACCCCCACCTGATGGGCTGGGACTACCCCAAGT

GCGACAGGGCCATGCCCAACATGCTGAGGATCATGGCCAGCCT

GGTGCTGGCCAGGAAGCACACCACCTGCTGCAGCCTGAGCCAC

AGGTTCTACAGGCTGGCCAACGAGTGCGCCCAGGTGCTGAGCG

AGATGGTGATGTGCGGCGGCAGCCTGTACGTGAAGCCCGGCGG

CACCAGCAGCGGCGACGCCACCACCGCCTACGCCAACAGCGTG

TTCAACATCTGCCAGGCCGTGACCGCCAACGTGAACGCCCTGCT

GAGCACCGACGGCAACAAGATCGCCGACAAGTACGTGAGGAAC

CTGCAGCACAGGCTGTACGAGTGCCTGTACAGGAACAGGGACG

TGGACACCGACTTCGTGAACGAGTTCTACGCCTACCTGAGGAAG

CACTTCAGCATGATGATCCTGAGCGACGACGCCGTGGTGTGCTT

CAACAGCACCTACGCCAGCCAGGGCCTGGTGGCCAGCATCAAG

AACTTCAAGAGCGTGCTGTACTACCAGAACAACGTGTTCATGAG

CGAGGCCAAGTGCTGGACCGAGACCGACCTGACCAAGGGCCCC

CACGAGTTCTGCAGCCAGCACACCATGCTGGTGAAGCAGGGCG

ACGACTACGTGTACCTGCCCTACCCCGACCCCAGCAGGATCCTG

GGCGCCGGCTGCTTCGTGGACGACATCGTGAAGACCGACGGCA

CCCTGATGATCGAGAGGTTCGTGAGCCTGGCCATCGACGCCTAC

CCCCTGACCAAGCACCCCAACCAGGAGTACGCCGACGTGTTCC

ACCTGTACCTGCAGTACATCAGGAAGCTGCACGACGAGCTGACC

GGCCACATGCTGGACATGTACAGCGTGATGCTGACCAACGACAA

CACCAGCAGGTACTGGGAGCCCGAGTTCTACGAGGCCATGTAC

ACCCCCCACACCGTGCTGCAG

NSP13
GCCGTGGGCGCCTGCGTGCTGTGCAACAGCCAGACCAGCCTGA
356

GGTGCGGCGCCTGCATCAGGAGGCCCTTCCTGTGCTGCAAGTG

CTGCTACGACCACGTGATCAGCACCAGCCACAAGCTGGTGCTGA

GCGTGAACCCCTACGTGTGCAACGCCCCCGGCTGCGACGTGAC

CGACGTGACCCAGCTGTACCTGGGCGGCATGAGCTACTACTGC

AAGAGCCACAAGCCCCCCATCAGCTTCCCCCTGTGCGCCAACG

GCCAGGTGTTCGGCCTGTACAAGAACACCTGCGTGGGCAGCGA

CAACGTGACCGACTTCAACGCCATCGCCACCTGCGACTGGACCA

ACGCCGGCGACTACATCCTGGCCAACACCTGCACCGAGAGGCT

GAAGCTGTTCGCCGCCGAGACCCTGAAGGCCACCGAGGAGACC

TTCAAGCTGAGCTACGGCATCGCCACCGTGAGGGAGGTGCTGA

GCGACAGGGAGCTGCACCTGAGCTGGGAGGTGGGCAAGCCCA

GGCCCCCCCTGAACAGGAACTACGTGTTCACCGGCTACAGGGT

GACCAAGAACAGCAAGGTGCAGATCGGCGAGTACACCTTCGAG

AAGGGCGACTACGGCGACGCCGTGGTGTACAGGGGCACCACCA

CCTACAAGCTGAACGTGGGCGACTACTTCGTGCTGACCAGCCAC

ACCGTGATGCCCCTGAGCGCCCCCACCCTGGTGCCCCAGGAGC

ACTACGTGAGGATCACCGGCCTGTACCCCACCCTGAACATCAGC

GACGAGTTCAGCAGCAACGTGGCCAACTACCAGAAGGTGGGCA

TGCAGAAGTACAGCACCCTGCAGGGCCCCCCCGGCACCGGCAA

GAGCCACTTCGCCATCGGCCTGGCCCTGTACTACCCCAGCGCC

AGGATCGTGTACACCGCCTGCAGCCACGCCGCCGTGGACGCCC

TGTGCGAGAAGGCCCTGAAGTACCTGCCCATCGACAAGTGCAG

CAGGATCATCCCCGCCAGGGCCAGGGTGGAGTGCTTCGACAAG

TTCAAGGTGAACAGCACCCTGGAGCAGTACGTGTTCTGCACCGT

GAACGCCCTGCCCGAGACCACCGCCGACATCGTGGTGTTCGAC

GAGATCAGCATGGCCACCAACTACGACCTGAGCGTGGTGAACG

CCAGGCTGAGGGCCAAGCACTACGTGTACATCGGCGACCCCGC

CCAGCTGCCCGCCCCCAGGACCCTGCTGACCAAGGGCACCCTG

GAGCCCGAGTACTTCAACAGCGTGTGCAGGCTGATGAAGACCAT

CGGCCCCGACATGTTCCTGGGCACCTGCAGGAGGTGCCCCGCC

GAGATCGTGGACACCGTGAGCGCCCTGGTGTACGACAACAAGC

TGAAGGCCCACAAGGACAAGAGCGCCCAGTGCTTCAAGATGTTC

TACAAGGGCGTGATCACCCACGACGTGAGCAGCGCCATCAACA

GGCCCCAGATCGGCGTGGTGAGGGAGTTCCTGACCAGGAACCC

CGCCTGGAGGAAGGCCGTGTTCATCAGCCCCTACAACAGCCAG

AACGCCGTGGCCAGCAAGATCCTGGGCCTGCCCACCCAGACCG

TGGACAGCAGCCAGGGCAGCGAGTACGACTACGTGATCTTCAC

CCAGACCACCGAGACCGCCCACAGCTGCAACGTGAACAGGTTC

AACGTGGCCATCACCAGGGCCAAGGTGGGCATCCTGTGCATCAT

GAGCGACAGGGACCTGTACGACAAGCTGCAGTTCACCAGCCTG

GAGATCCCCAGGAGGAACGTGGCCACCCTGCAG

NSP14
GCCGAGAACGTGACCGGCCTGTTCAAGGACTGCAGCAAGGTGA
357

TCACCGGCCTGCACCCCACCCAGGCCCCCACCCACCTGAGCGT

GGACACCAAGTTCAAGACCGAGGGCCTGTGCGTGGACATCCCC

GGCATCCCCAAGGACATGACCTACAGGAGGCTGATCAGCATGAT

GGGCTTCAAGATGAACTACCAGGTGAACGGCTACCCCAACATGT

TCATCACCAGGGAGGAGGCCATCAGGCACGTGAGGGCCTGGAT

CGGCTTCGACGTGGAGGGCTGCCACGCCACCAGGGAGGCCGT

GGGCACCAACCTGCCCCTGCAGCTGGGCTTCAGCACCGGCGTG

AACCTGGTGGCCGTGCCCACCGGCTACGTGGACACCCCCAACA

ACACCGACTTCAGCAGGGTGAGCGCCAAGCCCCCCCCCGGCGA

CCAGTTCAAGCACCTGATCCCCCTGATGTACAAGGGCCTGCCCT

GGAACGTGGTGAGGATCAAGATCGTGCAGATGCTGAGCGACAC

CCTGAAGAACCTGAGCGACAGGGTGGTGTTCGTGCTGTGGGCC

CACGGCTTCGAGCTGACCAGCATGAAGTACTTCGTGAAGATCGG

CCCCGAGAGGACCTGCTGCCTGTGCGACAGGAGGGCCACCTGC

TTCAGCACCGCCAGCGACACCTACGCCTGCTGGCACCACAGCAT

CGGCTTCGACTACGTGTACAACCCCTTCATGATCGACGTGCAGC

AGTGGGGCTTCACCGGCAACCTGCAGAGCAACCACGACCTGTA

CTGCCAGGTGCACGGCAACGCCCACGTGGCCAGCTGCGACGCC

ATCATGACCAGGTGCCTGGCCGTGCACGAGTGCTTCGTGAAGA

GGGTGGACTGGACCATCGAGTACCCCATCATCGGCGACGAGCT

GAAGATCAACGCCGCCTGCAGGAAGGTGCAGCACATGGTGGTG

AAGGCCGCCCTGCTGGCCGACAAGTTCCCCGTGCTGCACGACA

TCGGCAACCCCAAGGCCATCAAGTGCGTGCCCCAGGCCGACGT

GGAGTGGAAGTTCTACGACGCCCAGCCCTGCAGCGACAAGGCC

TACAAGATCGAGGAGCTGTTCTACAGCTACGCCACCCACAGCGA

CAAGTTCACCGACGGCGTGTGCCTGTTCTGGAACTGCAACGTGG

ACAGGTACCCCGCCAACAGCATCGTGTGCAGGTTCGACACCAG

GGTGCTGAGCAACCTGAACCTGCCCGGCTGCGACGGCGGCAGC

CTGTACGTGAACAAGCACGCCTTCCACACCCCCGCCTTCGACAA

GAGCGCCTTCGTGAACCTGAAGCAGCTGCCCTTCTTCTACTACA

GCGACAGCCCCTGCGAGAGCCACGGCAAGCAGGTGGTGAGCG

ACATCGACTACGTGCCCCTGAAGAGCGCCACCTGCATCACCAGG

TGCAACCTGGGCGGCGCCGTGTGCAGGCACCACGCCAACGAGT

ACAGGCTGTACCTGGACGCCTACAACATGATGATCAGCGCCGGC

TTCAGCCTGTGGGTGTACAAGCAGTTCGACACCTACAACCTGTG

GAACACCTTCACCAGGCTGCAG

NSP15
AGCCTGGAGAACGTGGCCTTCAACGTGGTGAACAAGGGCCACTT
358

CGACGGCCAGCAGGGCGAGGTGCCCGTGAGCATCATCAACAAC

ACCGTGTACACCAAGGTGGACGGCGTGGACGTGGAGCTGTTCG

AGAACAAGACCACCCTGCCCGTGAACGTGGCCTTCGAGCTGTG

GGCCAAGAGGAACATCAAGCCCGTGCCCGAGGTGAAGATCCTG

AACAACCTGGGCGTGGACATCGCCGCCAACACCGTGATCTGGG

ACTACAAGAGGGACGCCCCCGCCCACATCAGCACCATCGGCGT

GTGCAGCATGACCGACATCGCCAAGAAGCCCACCGAGACCATCT

GCGCCCCCCTGACCGTGTTCTTCGACGGCAGGGTGGACGGCCA

GGTGGACCTGTTCAGGAACGCCAGGAACGGCGTGCTGATCACC

GAGGGCAGCGTGAAGGGCCTGCAGCCCAGCGTGGGCCCCAAG

CAGGCCAGCCTGAACGGCGTGACCCTGATCGGCGAGGCCGTGA

AGACCCAGTTCAACTACTACAAGAAGGTGGACGGCGTGGTGCAG

CAGCTGCCCGAGACCTACTTCACCCAGAGCAGGAACCTGCAGG

AGTTCAAGCCCAGGAGCCAGATGGAGATCGACTTCCTGGAGCTG

GCCATGGACGAGTTCATCGAGAGGTACAAGCTGGAGGGCTACG

CCTTCGAGCACATCGTGTACGGCGACTTCAGCCACAGCCAGCTG

GGCGGCCTGCACCTGCTGATCGGCCTGGCCAAGAGGTTCAAGG

AGAGCCCCTTCGAGCTGGAGGACTTCATCCCCATGGACAGCACC

GTGAAGAACTACTTCATCACCGACGCCCAGACCGGCAGCAGCAA

GTGCGTGTGCAGCGTGATCGACCTGCTGCTGGACGACTTCGTG

GAGATCATCAAGAGCCAGGACCTGAGCGTGGTGAGCAAGGTGG

TGAAGGTGACCATCGACTACACCGAGATCAGCTTCATGCTGTGG

TGCAAGGACGGCCACGTGGAGACCTTCTACCCCAAGCTGCAG

NSP16
AGCAGCCAGGCCTGGCAGCCCGGCGTGGCCATGCCCAACCTGT
359

ACAAGATGCAGAGGATGCTGCTGGAGAAGTGCGACCTGCAGAA

CTACGGCGACAGCGCCACCCTGCCCAAGGGCATCATGATGAAC

GTGGCCAAGTACACCCAGCTGTGCCAGTACCTGAACACCCTGAC

CCTGGCCGTGCCCTACAACATGAGGGTGATCCACTTCGGCGCC

GGCAGCGACAAGGGCGTGGCCCCCGGCACCGCCGTGCTGAGG

CAGTGGCTGCCCACCGGCACCCTGCTGGTGGACAGCGACCTGA

ACGACTTCGTGAGCGACGCCGACAGCACCCTGATCGGCGACTG

CGCCACCGTGCACACCGCCAACAAGTGGGACCTGATCATCAGC

GACATGTACGACCCCAAGACCAAGAACGTGACCAAGGAGAACGA

CAGCAAGGAGGGCTTCTTCACCTACATCTGCGGCTTCATCCAGC

AGAAGCTGGCCCTGGGCGGCAGCGTGGCCATCAAGATCACCGA

GCACAGCTGGAACGCCGACCTGTACAAGCTGATGGGCCACTTC

GCCTGGTGGACCGCCTTCGTGACCAACGTGAACGCCAGCAGCA

GCGAGGCCTTCCTGATCGGCTGCAACTACCTGGGCAAGCCCAG

GGAGCAGATCGACGGCTACGTGATGCACGCCAACTACATCTTCT

GGAGGAACACCAACCCCATCCAGCTGAGCAGCTACAGCCTGTTC

GACATGAGCAAGTTCCCCCTGAAGCTGAGGGGCACCGCCGTGA

TGAGCCTGAAGGAGGGCCAGATCAACGACATGATCCTGAGCCT

GCTGAGCAAGGGCAGGCTGATCATCAGGGAGAACAACAGGGTG

GTGATCAGCAGCGACGTGCTGGTGAACAAC

The vaccine compositions described herein protects against disease caused by one or more coronavirus variants or coronavirus subvariants. In some embodiments, the coronavirus variants or coronavirus subvariants comprise past or Currently circulating coronavirus variants or coronavirus subvariants including but not limited to alpha, beta, gamma, delta, and omicron. In other embodiments, the coronavirus variants or coronavirus subvariants comprise future variants or future subvariants of human and animal coronavirus.

The vaccine compositions described herein may also protect against infection and reinfection of coronavirus variants or coronavirus subvariants. In some embodiments, the coronavirus variants or coronavirus subvariants comprise past or currently circulating coronavirus variants or coronavirus subvariants including but not limited to alpha, beta, gamma, delta, and omicron. In other embodiments, the coronavirus variants or coronavirus subvariants comprise future variants or future Subvariants of human and animal coronavirus.

The vaccine compositions described herein protects against infection or reinfection of one or more coronavirus variant or coronavirus subvariant. In some embodiments, the vaccine composition described herein against infection or reinfection of multiple coronavirus variants or coronavirus subvariants. In other embodiments, the vaccine composition described herein composition protects against infection or re-infection caused by one coronavirus variants or coronavirus subvariants.

In some embodiments, the vaccine composition induces strong and long-lasting protection mediated by antibodies (Abs), CD4+ T helper (Th1) cells, and/or CD8+ cytotoxic T-cells (CTL).

Molecular Adjuvants and T Cell Enhancements

In certain embodiments, the vaccine composition comprises a molecular adjuvant and/or one or more T Cell enhancement compositions. The adjuvant and/or enhancement compositions may help improve the immunogenicity and/or long-term memory of the vaccine composition. Non-limiting examples of molecular adjuvants include CpG, such as a CpG polymer, and flagellin,

In some embodiments, the vaccine composition comprises a T cell attracting chemokine. The T cell attracting chemokine helps pull the T cells from the circulation to the appropriate tissues, e.g., the lungs, heart, kidney, and brain. Non-limiting examples of T cell attracting chemokines include CCL5, CXCL9, CXCL10, CXCL11, CCL25, CCL28, CXCL14, CXCL17, or a combination thereof.

In some embodiments, the vaccine composition comprises a composition that promotes T cell proliferation. Non-limiting examples of compositions that promote T cell proliferation include IL-7, IL-15, IL-2, or a combination thereof.

In some embodiments, the vaccine composition comprises a composition that promotes T cell homing in the lungs. Non-limiting examples of compositions that promote T cell homing include CCL25, CCL28, CXCL14, CXCL17 or a combination thereof.

In certain embodiments, the molecular adjuvant and/or the T cell attracting chemokine and/or the composition that promotes T cell proliferation are delivered with a separate antigen delivery system from the large sequences.

Table 11 shows non-limiting examples of T-cell enhancements that may be used to create a vaccine composition described herein.

TABLE 11

T-cell

SEQ ID

enhancement
Sequence
NO:

CXCL11
ATGAACAGGAAGGTGACCGCCATCGCCCTGGCCGCCATCATCTGG
160

GCCACCGCCGCCCAGGGCTTCCTGATGTTCAAGCAGGGCAGGTGC

CTGTGCATCGGCCCCGGCATGAAGGCCGTGAAGATGGCCGAGATC

GAGAAGGCCAGCGTGATCTACCCCAGCAACGGCTGCGACAAGGTG

GAGGTGATCGTGACCATGAAGGCCCACAAGAGGCAGAGGTGCCTG

GACCCCAGGAGCAAGCAGGCCAGGCTGATCATGCAGGCCATCGAG

AAGAAGAACTTCCTGAGGAGGCAGAACATGTGA

CCL5
ATGAAGGTCTCCGCGGCAGCCCTCGCTGTCATCCTCATTGCTACTG
161

CCCTCTGCGCTCCTGCATCTGCCTCCCCATATTCCTCGGACACCAC

ACCCTGCTGCTTTGCCTACATTGCCCGCCCACTGCCCCGTGCCCAC

ATCAAGGAGTATTTCTACACCAGTGGCAAGTGCTCCAACCCAGCAG

TCGTCCACAGGTCAAGGATGCCAAAGAGAGAGGGACAGCAAGTCTG

GCAGGATTTCCTGTATGACTCCCGGCTGAACAAGGGCAAGCTTTGT

CACCCGAAAGAACCGCCAAGTGTGTGCCAACCCAGAGAAGAAATGG

GTTCGGGAGTACATCAACTCTTTGGAGATGAGCTAGGATGGAGAGT

CCTTGAACCTGAACTTACACAAATTTGCCTGTTTCTGCTTGCTCTTGT

CCTAGCTTGGGAGGCTTCCCCTCACTATCCTACCCCACCCGCTCCT

TGA

CXCL9
ATGAAGAAAAGTGGTGTTCTTTTCCTCTTGGGCATCATCTTGCTGGT
162

TCTGATTGGAGTGCAAGGAACCCCAGTAGTGAGAAAGGGTCGCTGT

TCCTGCATCAGCACCAACCAAGGGACTATCCACCTACAATCCTTGAA

AGACCTTAAACAATTTGCCCCAAGCCCTTCCTGCGAGAAAATTGAAA

TCATTGCTACACTGAAGAATGGAGTTCAAACATGTCTAAACCCAGAT

TCAGCAGATGTGAAGGAACTGATTAAAAAGTGGGAGAAACAGGTCA

GCCAAAAGAAAAAGCAAAAGAATGGGAAAAAACATGAAAAAAAGAAA

GTTCTGAAAGTTCGAAAATCTCAACGTTCTCGTCAAAAGAAGACTAC

ATAA

CXCL10
ATGAATCAAACTGCCATTCTGATTTGCTGCCTTATCTTTCTGACTCTA
163

AGTGGCATTCAAGGAGTACCTCTCTCTAGAACTGTACGCTGTACCTG

CATCAGCATTAGTAATCAACCTGTTAATCCAAGGTCTTTAGAAAAACT

TGAAATTATTCCTGCAAGCCAATTTTGTCCACGTGTTGAGATCATTG

CTACAATGAAAAAGAAGGGTGAGAAGAGATGTCTGAATCCAGAATC

GAAGGCCATCAAGAATTTACTGAAAGCAGTTAGCAAGGAAAGGTCTA

AAAGATCTCCTTAA

CXCL14
ATGAGGCTCCTGGCGGCCGCGCTGCTCCTGCTGCTGCTGGCGCTG
164

TACACCGCGCGTGTGGACGGGTCCAAATGCAAGTGCTCCCGGAAG

GGACCCAAGATCCGCTACAGCGACGTGAAGAAGCTGGAAATGAAGC

CAAAGTACCCGCACTGCGAGGAGAAGATGGTTATCATCACCACCAA

GAGCGTGTCCAGGTACCGAGGTCAGGAGCACTGCCTGCACCCCAA

GCTGCAGAGCACCAAGCGCTTCATCAAGTGGTACAACGCCTGGAAC

GAGAAGCGCAGGGTCTACGAAGAATAG

CXCL17
ATGAAAGTTCTAATCTCTTCCCTCCTCCTGTTGCTGCCACTAATGCT
165

GATGTCCATGGTCTCTAGCAGCCTGAATCCAGGGGTCGCCAGAGGC

CACAGGGACCGAGGCCAGGCTTCTAGGAGATGGCTCCAGGAAGGC

GGCCAAGAATGTGAGTGCAAAGATTGGTTCCTGAGAGCCCCGAGAA

GAAAATTCATGACAGTGTCTGGGCTGCCAAAGAAGCAGTGCCCCTG

TGATCATTTCAAGGGCAATGTGAAGAAAACAAGACACCAAAGGCAC

CACAGAAAGCCAAACAAGCATTCCAGAGCCTGCCAGCAATTTCTCAA

ACAATGTCAGCTAAGAAGCTTTGCTCTGCCTTTGTAG

CCL25
ATGAACCTGTGGCTCCTGGCCTGCCTGGTGGCCGGCTTCCTGGGA
166

GCCTGGGCCCCCGCTGTCCACACCCAAGGTGTCTTTGAGGACTGCT

GCCTGGCCTACCACTACCCCATTGGGTGGGCTGTGCTCCGGCGCG

CCTGGACTTACCGGATCCAGGAGGTGAGCGGGAGCTGCAATCTGC

CTGCTGCGATATTCTACCTCCCCAAGAGACACAGGAAGGTGTGTGG

GAACCCCAAAAGCAGGGAGGTGCAGAGAGCCATGAAGCTCCTGGA

TGCTCGAAATAAGGTTTTTGCAAAGCTCCACCACAACACGCAGACCT

TCCAAGCAGGCCCTCATGCTGTAAAGAAGTTGAGTTCTGGAAACTC

CAAGTTATCATCGTCCAAGTTTAGCAATCCCATCAGCAGCAGTAAGA

GGAATGTCTCCCTCCTGATATCAGCTAATTCAGGACTGTGA

CCL28
ATGCAGCAGAGAGGACTCGCCATCGTGGCCTTGGCTGTCTGTGCG
167

GCCCTACATGCCTCAGAAGCCATACTTCCCATTGCCTCCAGCTGTTG

CACGGAGGTTTCACATCATATTTCCAGAAGGCTCCTGGAAAGAGTG

AATATGTGTCGCATCCAGAGAGCTGATGGGGATTGTGACTTGGCTG

CTGTCATCCTTCATGTCAAGCGCAGAAGAATCTGTGTCAGCCCGCA

CAACCATACTGTTAAGCAGTGGATGAAAGTGCAAGCTGCCAAGAAA

AATGGTAAAGGAAATGTTTGCCACAGGAAGAAACACCATGGCAAGA

GGAACAGTAACAGGGCACATCAGGGGAAACACGAAACATACGGCCA

TAAAACTCCTTATTAG

IL-7
ATGTTCCACGTGAGCTTCAGGTACATCTTCGGCATCCCCCCCCTGAT
168

CCTGGTGCTGCTGCCCGTGACCAGCAGCGAGTGCCACATCAAGGA

CAAGGAGGGCAAGGCCTACGAGAGCGTGCTGATGATCAGCATCGA

CGAGCTGGACAAGATGACCGGCACCGACAGCAACTGCCCCAACAA

CGAGCCCAACTTCTTCAGGAAGCACGTGTGCGACGACACCAAGGAG

GCCGCCTTCCTGAACAGGGCCGCCAGGAAGCTGAAGCAGTTCCTG

AAGATGAACATCAGCGAGGAGTTCAACGTGCACCTGCTGACCGTGA

GCCAGGGCACCCAGACCCTGGTGAACTGCACCAGCAAGGAGGAGA

AGAACGTGAAGGAGCAGAAGAAGAACGACGCCTGCTTCCTGAAGAG

GCTGCTGAGGGAGATCAAGACCTGCTGGAACAAGATCCTGAAGGG

CAGCATCTGA

IL-15
ATGAGAATTTCGAAACCACATTTGAGAAGTATTTCCATCCAGTGCTA
169

CTTGTGTTTACTTCTAAACAGTCATTTTCTAACTGAAGCTGGCATTCA

TGTCTTCATTTTGGGCTGTTTCAGTGCAGGGCTTCCTAAAACAGAAG

CCAACTGGGTGAATGTAATAAGTGATTTGAAAAAAATTGAAGATCTTA

TTCAATCTATGCATATTGATGCTACTTTATATACGGAAAGTGATGTTC

ACCCCAGTTGCAAAGTAACAGCAATGAAGTGCTTTCTCTTGGAGTTA

CAAGTTATTTCACTTGAGTCCGGAGATGCAAGTATTCATGATACAGT

AGAAAATCTGATCATCCTAGCAAACAACAGTTTGTCTTCTAATGGGA

ATGTAACAGAATCTGGATGCAAAGAATGTGAGGAACTGGAGGAAAA

AAATATTAAAGAATTTTTGCAGAGTTTTGTACATATTGTCCAAATGTT

CATCAACACTTCTTGA

IL-2
ATGTACAGGATGCAACTCCTGTCTTGCATTGCACTAAGTCTTGCACT
170

TGTCACAAACAGTGCACGTACTTCAAGTTCTACAAAGAAAACACAGC

TACAACTGGAGCATTTACTGCTGGATTTACAGATGATTTTGAATGGA

ATTAATAATTACAAGAATCCCAAACTCACCAGGATGCTCACATTTAAG

TTTTACATGCCCAAGAAGGCCACAGAACTGAAACATCTTCAGTGTCT

AGAAGAAGAACTCAAACCTCTGGAGGAAGTGCTAAATTTAGCTCAAA

GCAAAAACTTTCACTTAAGACCCAGGGACTTAATCAGCAATATCAAC

GTAATAGTTCTGGAACTAAAGGGATCTGAAACAACATTCATGTGTGA

ATATGCTGATGAGACAGCAACCATTGTAGAATTTCTGAACAGATGGA

TTACCTTTGTCAAAGCATCATCTCAACACTGACTTGA

In preferred embodiments, the T-cell enhancement compositions described herein (e.g. CXCL9, CXCL10, IL-7, IL-2) may be integrated into a separate delivery system from the vaccine compositions. In some embodiments, the T-cell enhancement compositions described herein (e.g. CXCL9, CXCL1G, IL-7, IL-2) may be integrated into the same delivery system as the vaccine compositions.

In certain embodiments, the vaccine composition comprises a tag. For example, in some embodiments, the vaccine composition comprises a His tag. The present invention is not limited to a His tag and includes other tags such as those known to one of ordinary skill in the art, such as a fluorescent tag (e g. GFP, YFP, etc.), etc.

Antigen Delivery System

The present invention also features vaccine compositions in the form of an antigen delivery system. Any appropriate antigen delivery system may be considered for delivery of the antigens described herein. The present invention is not limited to the antigen delivery systems described herein.

In certain embodiments, the antigen delivery system is for targeted delivery of the vaccine composition, e.g., for targeting to the tissues of the body where the virus replicates.

In certain embodiments, the antigen delivery system comprises adenoviruses such as but not limited to Ad5, Ad26, Ad35, etc., as well as carriers such as lipid nanoparticles, polymers, peptides, etc. In other embodiments, the antigen delivery system comprises a vesicular stomatitis virus (VSV) vector.

The present invention is not limited to adenovirus vector-based antigen delivery systems. In certain embodiments, the antigen delivery system comprises an adeno-associated virus vector-based antigen delivery system, such as but not limited to the adeno-associated virus vector type 9 (AAV9 serotype), AAV type 8 (AAV8 serotype), etc. In certain embodiments, the adeno-associated virus vectors used are tropic, e.g., tropic to lungs, brain, heart and kidney, e.g., the tissues of the body that express ACE2 receptors (FIG. 3A)). For example, AAV9 is known to be neurotropic, which would help the vaccine composition to be expressed in the brain.

In the antigen delivery system, the one or more large sequences are operatively linked to a promoter. In certain embodiments, the one or more large sequences are operatively linked to a generic promoter. For example, in certain embodiments, the one or more large sequences are operatively linked to a CMV promoter. In certain embodiments, the one or more large sequences are operatively linked to a CAG, EFIA, EFS, CBh, SFFV, MSCV, mPGK, hPGK, SV40, UBC, or another appropriate promoter.

In some embodiments, the one or more large sequences are operatively linked to a tissue-specific promoter (e.g., a lung-specific promoter). For example, the antigen may be operatively linked to a SpB promoter or a CD144 promoter.

As discussed, in certain embodiments, the vaccine composition comprises a molecular adjuvant. In certain embodiments, the molecular adjuvant is operatively linked to a generic promoter, e.g., as described above. In certain embodiments, the molecular adjuvant is operatively linked to a tissue-specific promoter, e.g., a lung-specific promoter, e.g., SpB or CD144.

As discussed, in certain embodiments, the vaccine composition comprises a T cell attracting chemokine. In certain embodiments, the T cell attracting chemokine is operatively linked to a generic promoter, e.g., as described above. In certain embodiments, the T cell attracting chemokine is operatively linked to a tissue-specific promoter; e.g.; a lung-specific promoter, e.g.; CpB or CD144,

As discussed, in certain embodiments, the vaccine composition comprises a composition for promoting T cell proliferation. In certain embodiments, the composition for promoting T cell proliferation is operatively linked to a generic promoter, e.g., as described above. In certain embodiments, the composition for promoting T cell proliferation is operatively linked to a tissue-specific promoter, e.g., a lung-specific promoter, e.g.; SpB or CD144.

Table 12 shows non-limiting examples of promoters that may be used to create a vaccine composition described herein.

TABLE 12

SEQ ID

Promoter
Sequence
NO:

CAG
CTCGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCAT
171

TAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAAT

GGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATA

ATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCA

ATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTG

TATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGC

CCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTG

GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGAGC

CCCACGTTCTGCTTCACTCTCCCCATCTCCCCCCCCTCCCCACCCCCAA

TTTTGTATTTATTTATTTTTTAATTATTTTGTGCAGCGATGGGGGCGGGG

GGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGGGGCGAGGGG

CGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCAGAGCG

GCGCGCTCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGCGGCGG

CCCTATAAAAAGCGAAGCGCGCGGCGGGCGGGAGTCGCTGCGCGCTG

CCTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCGCGCCGCCCGCCCC

GGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGGGACGGC

CCTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGGCTTGTTTC

TTTTCTGTGGCTGCGTGAAAGCCTTGAGGGGCTCCGGGAGGGCCCTTT

GTGCGGGGGGAGCGGCTCGGGGGGTGCGTGCGTGTGTGTGTGCGTGG

GGAGCGCCGCGTGCGGCTCCGCGCTGCCCGGCGGCTGTGAGCGCTGC

GGGCGCGGCGCGGGGCTTTGTGCGCTCCGCAGTGTGCGCGAGGGGAG

CGCGGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGGGCTGCGAGGG

GAACAAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGGGTGAGCAGGGG

GTGTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTGCACCCCCCTCCC

CGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGGGCTCCGTACGGG

GCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGGTGGCGGCAGGTG

GGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCCGGGGAGGGCTCG

GGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGCGGCTGTCGAGGCG

CGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCGTGCGAGAGGGCGC

AGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCGAAATCTGGGAGGC

GCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGAAGCGGTGCGGCGC

CGGCAGGAAGGAAATGGGCGGGGAGGGCCTTCGTGCGTCGCCGCGCC

GCCGTCCCCTTCTCCCTCTCCAGCCTCGGGGCTGTCCGCGGGGGGAC

GGCTGCCTTCGGGGGGGACGGGGCAGGGCGGGGTTCGGCTTCTGGCG

TGTGACCGGCGGCTCTAGAGCCTCTGCTAACCATGTTCATGCCTTCTTC

TTTTTCCTACAGCTCCTGGGCAACGTGCTGGTTATTGTGCTGTCTCATCA

TTTGGCAAAGAATTG

CMV
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATAT
172

GGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCG

CCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAG

TAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACG

GTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACG

CCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCC

AGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTA

GTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGC

GTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGA

CGTCAATGGGAGTTTGTTGGCACCAAAATCAACGGGACTTTCCAAAAT

GTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTAC

GGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTCAGATC

SP-B
GTATAGGGCTGTCTGGGAGCCACTCCAGGGCCACAGAAATCTTGTCTCT
173

GACTCAGGGTATTTTGTTTTCTGTTTTGTGTAAATGCTCTTCTGACTAATG

CAAACCATGTGTCCATAGAACCAGAAGATTTTTCCAGGGGAAAAGGTAA

GGAGGTGGTGAGAGTGTCCTGGGTCTGCCCTTCCAGGGCTTGCCCTGG

GTTAAGAGCCAGGCAGGAAGCTCTCAAGAGCATTGCTCAAGAGTAGAG

GGGGCCTGGGAGGCCCAGGGAGGGGATGGGAGGGGAACACCCAGGC

TGCCCCCAACCAGATGCCCTCCACCCTCCTCAACCTCCCTCCCACGGC

CTGGAGAGGTGGGACCAGGTATGGAGGCTTGAGAGCCCCTGGTTGGAG

GAAGCCACAAGTCCAGGAACATGGGAGTCTGGGCAGGGGGCAAAGGA

GGCAGGAACAGGCCATCAGCCAGGACAGGTGGTAAGGCAGGCAGGAG

TGTTCCTGCTGGGAAAAGGTGGGATCAAGCACCTGGAGGGCTCTTCAG

AGCAAAGACAAACACTGAGGTCGCTGCCACTCCTACAGAGCCCCCACG

CCCCGCCCAGCTATAAGGGGCCATGCACCAAGCAGGGTACCCAGGCTG

CAGAGGTGCC

CD144
CATCCATGCCCATGGCCTCAGATGCCAGCCATAAGCTGTTGGGTTCCAA
174

ACCTCGACTCCAGGCTGGACTCACCCCTGTCTCCCCCACCAGCCTGAC

ACCTCCACCTGGGTATCTAACGAGCATCTCAAACTCAACCTGCCTGAGA

CAGAGGAATCACTATCCCCTCCTCCTCCAAAAATATCCTTCCATCACACT

CCCCATCTTGTGCTCTGATTTACTAAACGGCCCTGGGCCCTCTCTTTCTC

AGGGTCTCTGCTTGCCCAGCTATATAATAAAACAAGTTTGGGACTTCCCA

ACCATTCACCCATGGAAAAACAGAAGCAACTCTTCAAAGGACAGATTCC

CAGGATCTGCCCTGGGAGATTCCAAATCAGTTGATCTGGGGTGAGCCCA

GTCCTCTGTAGTTTTTAGAAGCTCCTCCTATGTCTCTCCTGGTCAGCAGA

ATCTTGGCCCCTCCCTTCCCCCCAGCCTCTTGGTTCTTCTGGGCTCTGA

TCCAGCCTCAGCGTCACTGTCTTCCACGCCCCTCTTTGATTCTCGTTTAT

GTCAAAAGCCTTGTGAGGATGAGGCTGTGATTATCCCCATTTACAGAT

GAGGAAACTGTGGCTCCAGGATGACACAACTGGCCAGAGGTCACATCA

GAAGCAGAGCTGGGTCACTTGACTCCACCCAATATCCCTAAATGCAAAC

ATCCCCTACAGACCGAGGCTGGCACCTTAGAGCTGGAGTCCATGCCCG

CTCTGACCAGGAGAAGCCAACCTGGTCCTCCAGAGCCAAGAGCTTCTGT

CCCTTTCCCATCTCCTGAAGCCTCCCTGTCACCTTTAAAGTCCATTCCCA

CAAAGACATCATGGGATCACCACAGAAAATCAAGCTCTGGGGCTAGGCT

GACCCCAGCTAGATTTTTGGCTCTTTTATACCCCAGCTGGGTGGACAAG

CACCTTAAACCCGCTGAGCCTCAGCTTCCCGGGCTATAAAATGGGGGTG

ATGACACCTGCCTGTAGCATTCCAAGGAGGGTTAAATGTGATGCTGCAG

CCAAGGGTCCCCACAGCCAGGCTCTTTGCAGGTGCTGGGTTCAGAGTC

CCAGAGCTGAGGCCGGGAGTAGGGGTTCAAGTGGGGTGCCCCAGGCA

GGGTCCAGTGCCAGCCCTCTGTGGAGACAGCCATCCGGGGCCGAGGC

AGCCGCCCACCGCAGGGCCTGCCTATCTGCAGCCAGCCCAGCCCTCAC

AAAGGAACAATAACAGGAAACCATCCCAGGGGGAAGTGGGCCAGGGCC

AGCTGGAAAACCTGAAGGGGAGGCAGCCAGGCCTCCCTCGCCAGCGG

GGTGTGGCTCCCCTCCAAAGACGGTCGGCTGACAGGCTCCACAGAGCT

CCACTCACGCTCAGCCCTGGACGGACAGGCAGTCCAACGGAACAGAAA

CATCCCTCAGCCCACAGGCACGGTGAGTGGGGGCTCCCACACTCCCCT

CCACCCCAAACCCGCCACCCTGCGCCCAAGATGGGAGGGTCCTCAGCT

TCCCCATCTGTAGAATGGGCATCGTCCCACTCCCATGACAGAGAGGCTC

C

wild type
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGC
175

native

leader

sequence

In certain embodiments, the T cell attracting chemokine and the composition that promotes T cell proliferation are driven by the same promoter (e.g., the T cell attracting chemokine and the composition that promotes T cell proliferation are synthesized as a peptide). In certain embodiments, the T cell attracting chemokine and the composition that promotes T cell proliferation are driven by different promoters. In certain embodiments, the antigen, the T cell attracting chemokine, and the composition that promotes T cell proliferation are driven by the same promoter. In certain embodiments, the antigen, the T cell attracting chemokine, and the composition that promotes T cell proliferation are driven by the different promoters. In certain embodiments, the T cell attracting chemokine and the composition that promotes T cell proliferation are driven by the same promoter, and the one or more large sequences are driven by a different promoter.

In some embodiments, the antigen delivery system comprises one or more linkers between the T cell attracting chemokine and the composition that promotes T cell proliferation. In certain embodiments, linkers are used between one or more of the epitopes. The linkers may allow for cleavage of the separate molecules (e.g., chemokine). For example, in some embodiments, a linker is positioned between IL-7 (or IL-2) and CCL5, CXCL9, CXCL10, CXCL11, CCL25, CCL28, CXCL14, CXCL17, etc. In some embodiments, a linker is positioned between IL-15 and CCL5, CXCL9, CXCL10, CXCL11, CCL25, CCL28, CXCL14, CXCL17, etc. In some embodiments, a linker is positioned between the antigen or large sequence and another composition, e.g., IL-15. IL-7, CCL5, CXCL9, CXCL10. CXCL11, CCL25, CCL28, CXCL14, CXCL17, etc. A non-limiting example of a linker is T2A, E2A, P2A (see Table 13), or the like. The composition may feature a different linker between each open reading frame.

TABLE 13

SEQ

SEQUENCE
ID NO:

T2A Linker
GGAAGCGGAGAGGGCAGGGGAAGTCTT
176

CTAACATGCGGGGACGTGGAGGAAAAT

CCCGGCCCC

E2A Linker
GGAAGCGGACAGTGTACTAATTATGCT
177

CTCTTGAAATTGGCTGGAGATGTTGAG

AGCAACCCAGGTCCC

P2A Linker
GGAAGCGGAGCCACGAACTTCTCTCTG
178

TTAAAGCAAGCAGGAGATGTTGAAGAA

AACCCCGGGCCT

GCCGCCTAC
179

GGCCCCGGCCCCGGC
180

6-His Tag
CATCACCATCACCATCAC
181

The present invention includes mRNA sequences encoding any of the vaccine compositions or portions thereof herein, e.g., a molecular adjuvant, a T cell enhancement, etc. The present invention also includes modified mRNA sequences encoding any of the vaccine compositions or portions thereof herein. The present invention also includes DNA sequence encoding any of the vaccine compositions or portions thereof herein.

In certain embodiments, nucleic acids of a vaccine composition herein are chemically modified. In some embodiments, the nucleic acids of a vaccine composition therein are unmodified. In some embodiments, all or a portion of the uracil in the open reading frame has a chemical modification. In some embodiments, a chemical modification is in the 5-position of the uracil. In some embodiments, a chemical modification is a N1-methyl pseudouridine. In some embodiments, all or a portion of the uracil in the open reading frame has a N1-methyl pseudouridine in the 5-position of the uracil.

In certain embodiments, an open reading frame of a vaccine composition herein encodes one antigen or epitopes. In some embodiments, an open reading frame of a vaccine composition herein encodes two or more antigens or epitopes. In some embodiments, an open reading frame of a vaccine composition herein encodes five or more antigens or epitopes. In some embodiments, an open reading frame of a vaccine composition herein encodes ten or more antigens or epitopes. In some embodiments, an open reading frame of a vaccine composition herein encodes 50 or more antigens or epitopes.

Methods

In some embodiments, the method comprises determining one or more conserved large sequences that are derived from coronavirus sequences (e.g., SARS-CoV-2, variants, common cold coronaviruses, previously known coronavirus strains, animal coronaviruses, etc.). The method may comprise selecting at least one large conserved sequence and synthesizing an antigen (or antigens) comprising the selected large conserved sequence(s). The method may comprise synthesizing a nucleotide composition (e.g., DNA, modified DNA. mRNA, modified mRNA, antigen delivery system, etc.) encoding the antigen comprising the selected large conserved sequence(s). In some embodiments, the method further comprises creating a vaccine composition comprising the antigen, nucleotide compositions, and/or antigen delivery system and a pharmaceutical carrier. In some embodiments, the large sequences comprise one or more conserved epitopes described herein, e.g., one or more conserved B-cell target epitopes and/or one or more conserved CD4+ T cell target epitopes and/or one or more conserved CD8+ T cell target epitopes.

In some embodiments, each of the large sequences are conserved among two or a combination of: at least two SARS-CoV-2 human strains in current circulation, at least one coronavirus that has caused a previous human outbreak, at least one coronavirus isolated from bats, at least one coronavirus isolated from pangolin, at least one coronavirus isolated from civet cats, at least one coronavirus strain isolated from mink, and at least one coronavirus strain isolated from camels or any other animal that is receptive to coronavirus.

As previously discussed, the compositions described herein, e.g., the antigens, the vaccine compositions, the antigen delivery systems, the chemokines, the adjuvants, etc. may be used to prevent a coronavirus disease in a subject. In some embodiments, the compositions described herein, e.g.; the antigens, the vaccine compositions, the antigen delivery systems, the chemokines, the adjuvants, etc. may be used to prevent a coronavirus infection prophylactically in a subject. In some embodiments, the compositions described herein, e.g., the antigens, the vaccine compositions, the antigen delivery systems, the chemokines, the adjuvants, etc. may elicit an immune response in a subject. In some embodiments, the compositions described herein, e.g., the antigens, the vaccine compositions, the antigen delivery systems, the chemokines, the adjuvants, etc. may prolong an immune response induced by the multi-epitope pan-coronavirus vaccine composition and increases T-cell migration to the lungs.

Methods for preventing a coronavirus disease in a subject may comprise administering to the subject a therapeutically effective amount of a pan-coronavirus vaccine composition according to the present invention. In some embodiments, the composition elicits an immune response in the subject. In some embodiments, the composition induces memory B and T cells. In some embodiments, the composition induces resident memory T cells (Trm), in some embodiments, the composition prevents virus replication, e.g., in the areas where the virus normally replicates such as lungs, brain, heart, and kidney. In some embodiments, the composition prevents a cytokine storm, e.g., in the areas where the virus normally replicates such as lungs, brain, heart, and kidney. In some embodiments, the composition prevents inflammation or an inflammatory response, e.g., in the areas where the virus normally replicates such as lungs, brain, heart, and kidney. In some embodiments, the composition improves homing and retention of T cells, e.g., in the areas where the virus normally replicates such as lungs, brain, heart, and kidney.

Methods for preventing a coronavirus infection prophylactically in a subject may comprise administering to the subject a prophylactically effective amount of a pan-coronavirus vaccine composition according to the present invention. In some embodiments, the composition elicits an immune response in the subject. In some embodiments, the composition induces memory B and T cells. In some embodiments, the composition induces resident memory T cells (Trm). In some embodiments, the composition prevents virus replication, e.g., in the areas where the virus normally replicates such as lungs, brain, heart, and kidney. In some embodiments, the composition prevents a cytokine storm, e.g., in the areas where the virus normally replicates such as lungs, brain, heart, and kidney. In some embodiments, the composition prevents inflammation or an inflammatory response, e.g., in the areas where the virus normally replicates such as lungs, brain, heart, and kidney. In some embodiments, the composition improves homing and retention of T cells, e.g., in the areas where the virus normally replicates such as lungs, brain, heart, and kidney.

Methods for eliciting an immune response in a subject may comprise administering to the subject a vaccine composition according to the present invention, wherein the composition elicits an immune response in the subject. In some embodiments, the composition induces memory B and T cells. In some embodiments, the composition induces resident memory T cells (Trm). In some embodiments, the composition prevents virus replication, e.g., in the areas where the virus normally replicates such as lungs, brain, heart, and kidney. In some embodiments, the composition prevents a cytokine storm, e.g., in the areas where the virus normally replicates such as lungs, brain, heart, and kidney. In some embodiments, the composition prevents inflammation or an inflammatory response, e.g., in the areas where the virus normally replicates such as lungs, brain, heart, and kidney. In some embodiments, the composition improves homing and retention of T cells, e.g., in the areas where the virus normally replicates such as lungs, brain, heart, and kidney.

Methods for prolonging an immune response induced by a vaccine composition of the present invention and increasing T cell migration to particular tissues (e.g., lung, brain, heart, kidney, etc.) may comprise co-expressing a T-cell attracting chemokine, a composition that promotes T cell proliferation, and a vaccine composition (e.g., antigen) according to the present invention.

Methods for prolonging the retention of memory T-cell into the lungs induced by a vaccine composition of the present invention and increasing virus-specific tissue resident memory T-cells (TRM cells) may comprise co-expressing a T-cell attracting chemokine, a composition that promotes T cell proliferation, and a vaccine composition (e.g., antigen) according to the present invention.

The vaccine composition may be administered through standard means, e.g., through an intravenous route (i.v.), an intranasal route (i.n.), or a sublingual route (s.l.) route.

In certain embodiments, the method comprises administering to the subject a second (e.g., booster) dose. The second dose may comprise the same vaccine composition or a different vaccine composition. Additional doses of one or more vaccine compositions may be administered.

Sequential Vaccine Delivery Methodology

In some embodiments, the present invention features a method of delivering the vaccine to induce heterologous immunity in a subject (e.g., prime/boost, see FIG. 25B and FIG. 26B). In some embodiments, the method comprises administering a first pan-coronavirus vaccine composition dose using a first delivery system. In further embodiments, the method comprises administering a second vaccine composition dose using a second delivery system. In some embodiments, the second composition is administered 8 days after administration of the first composition. In some embodiments, the second composition is administered 9 days after administration of the first composition. In some embodiments, the second composition is administered 10 days after administration of the first composition. In some embodiments, the second composition is administered 11 days after administration of the first composition. In some embodiments, the second composition is administered 12 days after administration of the first composition. In some embodiments, the second composition is administered 13 days after administration of the first composition. In some embodiments, the second composition is administered 14 days after administration of the first composition. In some embodiments, the second composition is administered from 14 to 30 days after administration of the first composition. In some embodiments, the second composition is administered from 30 to 60 days after administration of the first composition. In other embodiments, the first delivery system and the second delivery system are different. In some embodiments, the peptide vaccine composition is administered 14-days after the administration of the first vaccine composition dose. In some embodiments, the peptide vaccine composition is administered 30 or 60 days after the administration of the first vaccine composition dose.

In some embodiments, the first delivery system or the second delivery system comprises an mRNA, a modified mRNA or a peptide vector. In other embodiments, the peptide vector comprises adenovirus or an adeno-associated virus vector.

In some embodiments, the present invention features a method of delivering the vaccine to induce heterologous immunity in a subject (i.e. prime/pull, see FIG. 25A and FIG. 26A). In some embodiments, the method comprises administering a pan-coronavirus vaccine composition. In further embodiments, the method comprises administering at least one T-cell attracting chemokine after administering the pan-coronavirus vaccine composition. In some embodiments, the T-cell attracting chemokine is administered 8 days after the vaccine composition is administered. In some embodiments, the T-cell attracting chemokine is administered 9 days after the vaccine composition is administered. In some embodiments, the T-cell attracting chemokine is administered 10 days after the vaccine composition is administered. In some embodiments, the T-cell attracting chemokine is administered 11 days after the vaccine composition is administered. In some embodiments, the T-cell attracting chemokine is administered 12 days after the vaccine composition is administered. In some embodiments, the T-cell attracting chemokine is administered 13 days after the vaccine composition is administered. In some embodiments, the T-cell attracting chemokine is administered 14 days after the vaccine composition is administered. In some embodiments, the T-cell attracting chemokine is administered from 14 to 30 days after administration of the vaccine composition. In some embodiments, the T-cell attracting chemokine is administered from 30 to 60 days after administration of the vaccine composition. In some embodiments, the T cell-attracting chemokine composition is administered 8 to 14-days after the administration of the final vaccine composition dose. In some embodiments, the cell-attracting chemokine composition is administered 30 or 60 days after the administration of the final vaccine composition dose.

The present invention also features a novel “prime, pull, and boost” strategy. In other embodiments, the present invention features a method to increase the size and maintenance of lung-resident B-cells, CD4+ T cells and CD8+ T cells to protect against SARS-CoV-2 (FIG. 25D and FIG. 26D). In some embodiments, the method comprises administering a pan-coronavirus vaccine composition. In other embodiments, the method comprises administering at least one T-cell attracting chemokine after administering the pan-coronavirus vaccine composition. In further embodiments, the method comprises administering at least one cytokine after administering the T-cell attracting chemokine. In some embodiments, the T-cell attracting chemokine is administered 14 days after administering the pan-coronavirus composition. In other embodiments, the cytokine is administered 10 days after administering the T-cell attracting chemokine. In some embodiments, the T-cell attracting chemokine is administered 8 days after the vaccine composition is administered. In some embodiments, the T-cell attracting chemokine is administered 9 days after the vaccine composition is administered. In some embodiments, the T-cell attracting chemokine is administered 10 days after the vaccine composition is administered. In some embodiments, the T-cell attracting chemokine is administered 11 days after the vaccine composition is administered. In some embodiments, the T-cell attracting chemokine is administered 12 days after the vaccine composition is administered. In some embodiments, the T-cell attracting chemokine is administered 13 days after the vaccine composition is administered. In some embodiments, the T-cell attracting chemokine is administered 14 days after the vaccine composition is administered. In some embodiments, the T-cell attracting chemokine is administered from 14 to 30 days after administration of the vaccine composition. In some embodiments, the T-cell attracting chemokine is administered from 30 to 60 days after administration of the vaccine composition. In some embodiments, the cytokine is administered 8 days after administering the T-cell attracting chemokine. In some embodiments, the cytokine is administered 9 days after administering the T-cell attracting chemokine. In some embodiments, the cytokine is administered 10 days after administering the T-cell attracting chemokine. In some embodiments, the cytokine is administered 11 days after administering the T-cell attracting chemokine. In some embodiments, the cytokine is administered 12 days after administering the T-cell attracting chemokine. In some embodiments, the cytokine is administered 13 days after administering the T-cell attracting chemokine. In some embodiments, the cytokine is administered 14 days after administering the T-cell attracting chemokine. In some embodiments, the cytokine is administered from 14 to 30 days after administering the T-cell attracting chemokine. In some embodiments, the cytokine is administered from 30 to 60 days after administering the T-cell attracting chemokine. In some embodiments, the cytokine composition is administered 8 to 14-days after the administration of the T cell-attracting chemokine. In some embodiments, the cytokine composition is administered 30 or 60 days after the administration of the T cell-attracting chemokine.

The present invention further features a novel “prime, pull, and keep” strategy (FIG. 25C and FIG. 26C). In further embodiments, the present invention features a method to increase the size and maintenance of lung-resident B-cells, CD4+ T cells and CD8+ T cells to protect against SARS-CoV-2. In some embodiments, the method comprises administering a pan-coronavirus vaccine composition. In other embodiments, the method comprises administering at least one T-cell attracting chemokine after administering the pan-coronavirus vaccine composition. In further embodiments, the method comprises administering at least one mucosal chemokine after administering the T-cell attracting chemokine. In some embodiments, the T-cell attracting chemokine is administered 14 days after administering the pan-coronavirus composition. In other embodiments, the mucosal chemokines is administered 10 days after administering the T-cell attracting chemokine. In some embodiments, the T-cell attracting chemokine is administered 8 days after the vaccine composition is administered. In some embodiments, the T-cell attracting chemokine is administered 9 days after the vaccine composition is administered. In some embodiments, the T-cell attracting chemokine is administered 10 days after the vaccine composition is administered. In some embodiments, the T-cell attracting chemokine is administered 11 days after the vaccine composition is administered. In some embodiments, the T-cell attracting chemokine is administered 12 days after the vaccine composition is administered. In some embodiments, the T-cell attracting chemokine is administered 13 days after the vaccine composition is administered. In some embodiments, the T-cell attracting chemokine is administered 14 days after the vaccine composition is administered. In some embodiments, the T-cell attracting chemokine is administered from 14 to 30 days after administration of the vaccine composition. In some embodiments, the T-cell attracting chemokine is administered from 30 to 60 days after administration of the vaccine composition. In some embodiments, the mucosal chemokine is administered 8 days after administering the T-cell attracting chemokine. In some embodiments, the mucosal chemokine is administered 9 days after administering the T-cell attracting chemokine. In some embodiments, the mucosal chemokine is administered 10 days after administering the T-cell attracting chemokine. In some embodiments, the mucosal chemokine is administered 11 days after administering the T-cell attracting chemokine. In some embodiments, the mucosal chemokine is administered 12 days after administering the T-cell attracting chemokine. In some embodiments, the mucosal chemokine is administered 13 days after administering the T-cell attracting chemokine. In some embodiments, the mucosal chemokine is administered 14 days after administering the T-cell attracting chemokine. In some embodiments, the mucosal chemokine is administered from 14 to 30 days after administering the T-cell attracting chemokine. In some embodiments, the mucosal chemokine is administered from 30 to 60 days after administering the T-cell attracting chemokine. In some embodiments, the mucosal chemokine composition is administered 8 to 14-days after the administration of the T cell-attracting chemokine. In some embodiments, the mucosal cytokine composition is administered 30 or 60 days after the administration of the T cell-attracting chemokine.

In some embodiments, the mucosal chemokines may comprise CCL25, CCL28,CXCL14, CXCL17, or a combination thereof. In some embodiments, the T-cell attracting chemokines may comprise CCL5, CXCL9, CXCL10, CXC11, or a combination thereof. In some embodiments, the cytokines may comprise IL-15, IL-2, IL-7 or a combination thereof.

In some embodiments, the efficacy (or effectiveness) of a vaccine composition herein is greater than 60%. In some embodiments, the efficacy (or effectiveness) of a vaccine composition herein is greater than 70%. In some embodiments, the efficacy (or effectiveness) of a vaccine composition herein is greater than 80%. In some embodiments, the efficacy (or effectiveness) of a vaccine composition herein is greater than 90%. In some embodiments, the efficacy (or effectiveness) of a vaccine composition herein is greater than 95%.

Vaccine efficacy may be assessed using standard analyses (see, e.g., Weinberg et al., J Infect Dis. 2010 Jun. 1; 201(11):1607-10). For example, vaccine efficacy may be measured by double-blind, randomized, clinical controlled trials. Vaccine efficacy may be expressed as a proportionate reduction in disease attack rate (AR) between the unvaccinated (ARU) and vaccinated (ARV) study cohorts and can be calculated from the relative risk (RR) of disease among the vaccinated group with use of the following formulas: Efficacy=(ARU−ARV)/ARU×100; and Efficacy=(1−RR)×100.

Likewise, vaccine effectiveness may be assessed using standard analyses (see, e.g., Weinberg et al., J Infect Dis. 2010 Jun. 1; 201(11):1607-10). Vaccine effectiveness is an assessment of how a vaccine (which may have already proven to have high vaccine efficacy) reduces disease in a population. This measure can assess the net balance of benefits and adverse effects of a vaccination program, not just the vaccine itself, under natural field conditions rather than in a controlled clinical trial. Vaccine effectiveness is proportional to vaccine efficacy (potency) but is also affected by how well target groups in the population are immunized, as well as by other non-vaccine-related factors that influence the ‘real-world’ outcomes of hospitalizations, ambulatory visits, or costs. For example, a retrospective case control analysis may be used, in which the rates of vaccination among a set of infected cases and appropriate controls are compared. Vaccine effectiveness may be expressed as a rate difference, with use of the odds ratio (OR) for developing infection despite vaccination: Effectiveness=(1−OR)×100.

In some embodiments, the vaccine immunizes the subject against a coronavirus for up to 1 year. In some embodiments, the vaccine immunizes the subject against a coronavirus for up to 2 years. In some embodiments, the vaccine immunizes the subject against a coronavirus for more than 1 year, more than 2 years, more than 3 years, more than 4 years, or for 5-10 years.

In some embodiments, the subject is a young adult between the ages of about 20 years and about 50 years (e.g., about 20, 25, 30, 35, 40, 45 or 50 years old).

In some embodiments, the subject is an elderly subject about 60 years old, about 70 years old, or older (e.g., about 60, 65, 70, 75, 80, 85 or 90 years old).

In some embodiments, the subject is about 5 years old or younger. For example, the subject may be between the ages of about 1 year and about 5 years (e.g., about 1, 2, 3, 5 or 5 years), or between the ages of about 6 months and about 1 year (e.g., about 6, 7, 8, 9, 10, 11 or 12 months). In some embodiments, the subject is about 12 months or younger (e.g., 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2 months or 1 month). In some embodiments, the subject is about 6 months or younger.

In some embodiments, the subject was born full term (e.g., about 37-42 weeks). In some embodiments, the subject was born prematurely, for example, at about 36 weeks of gestation or earlier (e.g., about 36, 35, 34, 33, 32, 31, 30, 29, 28, 27, 26 or 25 weeks). For example, the subject may have been born at about 32 weeks of gestation or earlier. In some embodiments, the subject was born prematurely between about 32 weeks and about 36 weeks of gestation. In such subjects, a vaccine may be administered later in life, for example, at the age of about 6 months to about 5 years, or older.

In some embodiments, the subject is pregnant (e.g., in the first, second or third trimester) when administered a vaccine.

In some embodiments, the subject has a chronic pulmonary disease (e.g., chronic obstructive pulmonary disease (COPD) or asthma) or is at risk thereof. Two forms of COPD include chronic bronchitis, which involves a long-term cough with mucus, and emphysema, which involves damage to the lungs over time. Thus, a subject administered a vaccine may have chronic bronchitis or emphysema.

In some embodiments, the subject has been exposed to a coronavirus. In some embodiments, the subject is infected with a coronavirus. In some embodiments, the subject is at risk of infection by a coronavirus.

In some embodiments, the subject is immunocompromised (has an impaired immune system, e.g., has an immune disorder or autoimmune disorder).

Pharmaceutical Carriers

In certain embodiments, the vaccine composition further comprises a pharmaceutical carrier. Pharmaceutical carriers are well known to one of ordinary skill in the art. For example, in certain embodiments, the pharmaceutical carrier is selected from the group consisting of water, an alcohol, a natural or hardened oil, a natural or hardened wax, a calcium carbonate, a sodium carbonate, a calcium phosphate, kaolin, talc, lactose and combinations thereof. In some embodiments, the pharmaceutical carrier may comprise a lipid nanoparticle, an adenovirus vector, or an adeno-associated virus vector. In some embodiments, the vaccine composition is constructed using an adeno-associated virus vectors-based antigen delivery system.

Also provided herein is vaccine of any one of the foregoing paragraphs, formulated in a nanoparticle (e.g., a lipid nanoparticle). In some embodiments, the nanoparticle has a mean diameter of 50-200 nm. In some embodiments, the nanoparticle is a lipid nanoparticle. In some embodiments, the lipid nanoparticle comprises a cationic lipid, a PEG-modified lipid, a sterol and a non-cationic lipid. In some embodiments, the lipid nanoparticle comprises a molar ratio of about 20-60% cationic lipid, 0.5-15% PEG-modified lipid, 25-55% sterol, and 25% non-cationic lipid. In some embodiments, the cationic lipid is an ionizable cationic lipid, and the non-cationic lipid is a neutral lipid, and the sterol is a cholesterol. In some embodiments, the cationic lipid is selected from 2,2-dilinoleyl-4-dimethylaminoethyl-[1,3]-dioxolane (DLin-KC2-DMA), dilinoleyl-methyl-4-dimethylaminobutyrate (DLin-MC3-DMA), and di((Z)-non-2-en-1-yl) 9-((4-(dimethylamino)butanoyl)oxy)heptadecanedioate (L319).

Hybrid Vaccine Candidates

Referring now to FIGS. 30, 31, 32A, and 32B, the present invention may further feature a pan-coronavirus-influenza recombinant vaccine composition. The composition comprises at least a portion of a coronavirus spike (S) protein and at least a portion of an influenza hemagglutinin (HA) protein.

In some embodiments, the portion of an influenza hemagglutinin (HA) protein is highly conserved among human influenza viruses. The portion of an influenza hemagglutinin (HA) protein may be derived from one or more of: H1N1 virus strain, H3N2 virus strain, influenza B virus strains, or variants thereof.

In some embodiments, the H1N1 virus strains or variants are selected from 28566 available complete genome sequences in NCBI for hemagglutinin (HA) gene. Some of the prominent strains are: OK384178.1, OM642156.1, OM654386.1, OL840606.1, OK625377.1, OM865246.1, OM935941.1, OM642158.1, OM935953.1, MW840068.1, MW839847.1, MW839825.1, MW930730.1, MT227010.1, LC638096.1, LC638077.1, LC637999.1, and LC645067.1. In some embodiments, the H3N2 virus strains or variants are selected from 33620 available complete genome sequences in NCBI for hemagglutinin (HA) gene. Some of the prominent strains are: MZ005227.1, MW849238.1, MZ203409.1, MZ198318.1, MZ198312.1, MZ198295.1, MZ198289.1, MZ198265.1, MW789449.1, MW798370.1, MW790182.1, MW789645.1, MW789778.1, MW789685.1, MW789659.1, and MW790001.1. In some embodiments, the influenza B virus strains or variants are selected from 16596 available complete genome sequences in NCBI for hemagglutinin (HA) gene. Some of the prominent strains are: M10298.1, MT7385253, MT808048.1, MT056751.1, MT314641.1, MT874090.1, MT242979.1, MT315665.1, MT105564.0.1, MT057563.1, MT056955.1, MT243019.1, MT306916.1, MT057571.1, MT314793.1, MT343026.1, MT874109.1, MT243795.1, MT315769.1, and KX885875.1.

Table 14: Shows non-limiting examples of a portion of an influenza hemagglutinin (HA) protein that may be used in accordance with the present invention.

SEQ ID

Sequence
NO:

HA (nucleotide)
TTCGGAGCTATTGCTGGTTTCTTGGAAGGAGGATGGGAAGGAATGA
384

TTGCAGGTTGGCACGGATACACATCTCATGGAGCACATGGAGTAGC

AGTGGCAGCAGACCTTAAGAGTACCCA

HA
FGAIAGFLEGGWEGMIAGWHGYTSHGAHGVAVAADLKSTX
385

(amino acid)

HA-H1N1
ATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAA
386

TGCAGACACATTATGTATAGGTTATCATGCGAACAACTCAACAGACA

CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTT

AACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGAG

GGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGCTGGCTGGAT

CCTGGGAAATCCAGAGTGTGAATCACTCTCCACAGCAAGCTCATGG

TCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCC

AGGAGATTTCATCGATTATGAGGAGCTAAGAGAGCAATTGAGCTCA

GTGTCATCATTTGAAAGGTTTGAGATATTCCCCAAGACAAGTTCATG

GCCCAATCATGACTCGAACAAAGGTGTAACGACAGCATGTCCTCAT

GCTGGAGCAAAAAGCTTCTACAAAAATTTAATATGGCTAGTTAAAAA

AGGAAATTCATACCCAAAGCTCAGCAAATCCTACATTAATGATAAAG

GGAAAGAAGTCCTCGTGCTATGGGGCATTCACCATCCACCTACTAG

TGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTTTTG

TGGGGACATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAAT

AAGGCCCAAAGTGAGGGATCAAGAAGGGAGAATGAACTATTACTGG

ACACTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAA

ATCTAGTGGTACCGAGATATGCATTCGCAATGGAAAGAAATGCTGG

ATCTGGTATTATCATTTCAGATACACCAGTCCACGATTGCAATACAA

CTTGTCAGACACCCAAGGGTGCTATAAACACCAGCCTCCCATTTCA

GAACATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAA

GCACAAAATTGAGACTGGCCACAGGATTGAGGAATGTCCCGTCCAT

TCAATCTAGAGGCCTATTTGGGGCCATTGCCGGTTTCATTGAAGGG

GGGTGGACAGGGATGGTAGATGGATGGTACGGTTATCACCATCAAA

ATGAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACACAGA

ATGCCATTGACGAGATTACTAACAAAGTAAACTCTGTTATTGAAAAAA

TGAATACACAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAA

AAAAGAATAGAGAATTTAAATAAAAAAGTTGATGATGGTTTCCTGGA

CATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAA

GAACTTTGGACTACCACGATTCAAAGGTGAAGAACTTATATGAAAAG

GTAAGAAGCCAGTTAAAAAACAATGCCAAGGAAATTGGAAACGGCT

GCTTTGAATTTTACCACAAATGTGATAACACGTGCATGGAAAGTGTC

AAAAATGGGACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATT

AAACAGAGAAGAAATAGATGGGGTAAAGCTGGAATCAACAAGGATT

TACCAGATTTTGGCGATCTATTCAACCGTCGCCAGTTCATTGGTACT

GGTAGTCTCCCTGGGGGCAATCAGTTTCTGGATGTGCTCTAATGGG

TCTCTACAGTGTAGAATATGTATTTAA

HA H3N2
AGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCT
387

TTGAGCTACATTCTATGTCTGGTTTTCGCTCAAAAACTTCCTGGAAAT

GACAATAGCACTGCAACGCTGTGCCTTGGGCACCATGCAGTACCAA

ACGGAACGATAGTGAAAACAATCACGAATGACCAAATTGAAGTTACT

AATGCTACTGAGCTGGTTCAGAATTCCTCAATAGGTGAAATATGCGA

CAGTCCTCATCAGATCCTTGATGGAGAAAACTGCACACTAATAGATG

CTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAAGAAATGG

GACCTTTTTGTTGAACGAAGCAAAGCCTACAGCAACTGTTACCCTTA

TGATGTGCCGGATTATGCCTCCCTTAGGTCACTAGTTGCCTCATCCG

GCACACTGGAGTTTAACAATGAAAGCTTCAATTGGGCTGGAGTCACT

CAAAACGGAACAAGTTCTGCTTGCATAAGGGGATCTAATAGTAGTTT

CTTTAGTAGATTAAATTGGTTGACCCACTTAAACTTCAAGTACCCAG

CATTGAACGTGACTATGCCAAACAATGAACAATTTGACAAATTGTAC

ATTTGGGGGGTTCACCACCCGGGTACGGACAAGGACCAAATCTTCC

TGTATGCTCAATCATCAGGAAGAATCACAGTATCTACCAAAAGAAGC

CAACAAGCTGTAATCCCGAATATCGGATCTAGACCCAGAATAAGGAA

TATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAACCGGGAG

ACATACTTTTGATTAACAGCACAGGGAATCTAATTGCTCCTAGGGGT

TACTTCAAAATACGAAGTGGGAAAAGCTCAATAATGAGATCAGATGC

ACCCATTGGCAAATGCAAGTCTGAATGCATCACTCCAAATGGAAGCA

TTCCCAATGACAAACCATTCCAAAATGTAAACAGGATCACATACGGG

GCCTGTCCCAGATATGTTAAGCAAAGCACTCTGAAATTGGCAACAG

GAATGCGAAATGTACCAGAGAAACAAACTAGAGGCATATTTGGCGC

AATAGCGGGTTTCATAGAAAATGGTTGGGAGGGAATGGTGGATGGT

TGGTACGGTTTCAGGCATCAAAATTCTGAGGGAAGAGGACAAGCAG

CAGATCTCAAAAGCACTCAAGCAGCAATCGATCAAATCAATGGGAA

GCTGAATCGATTGATCGGGAAAACCAACGAGAAATTCCATCAGATTG

AGAAAGAATTCTCAGAAGTAGAAGGGAGAATTCAGGACCTTGAGAA

ATATGTTGAGGACACAAAAATAGATCTCTGGTCATACAACGCAGAGC

TTCTTGTTGCCCTGGAAAACCAACATACAATTGATCTAACTGACTCA

GAAATGAACAAACTGTTTGAAAAAACAAAGAAGCAACTGAGGGAAAA

TGCTGAGGATATGGGCAATGGTTGTTTCAAAATATACCACAAATGTG

ACAATGCCTGCATAGGATCAATCAGAAATGGAACTTATGACCACGAT

GTATACAGGGATGAAGCATTAAACAACCGGTTCCAGATCAAGGGAG

TTGAGCTGAAGTCAGGGTACAAAGATTGGATCCTATGGATTTCCTTT

GCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCATCATG

TGGGCCTGCCAAAAGGGCAACATTAGGTGCAACATTTGCATTTGAG

TGCATTAATTAAAAACACCCTTGTTTCTACT

HA influenza B
ATTTTCTAATATCCACAAAATGAAGGCAATAATTGTACTACTCATGGT
388

AGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACATCGTCAA

ACTCACCACATGTCGTCAAAACTGCTACTCAAGGGGAGGTCAACGT

GACCGGTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTG

CAAATCTCAAAGGAACAGAAACCAGGGGGAAACTATGCCCAAAATG

CCTCAACTGCACAGATCTGGATGTAGCCTTGGGCAGACCAAAATGC

ACAGGGAAAATACCCTCTGCAAGGGTTTCAATACTCCATGAAGTCAG

ACCTGTTACATCTGGGTGCTTTCCTATAATGCACGATAGAACAAAAA

TTAGACAGCTGCCTAACCTTCTCCGAGGATACGAACATGTCAGGTTA

TCAACTCACAACGTTATCAATGCAAAAGATGCACCAGGAAGACCCTA

CGAAATTGGAACCTCAGGGTCTTGCCCTAACATTACCAATGGAAAC

GGATTCTTCGCAACAATGGCTTGGGCCGTCCCAAAAAACAAAACAG

CAACAAATCCATTAACAATAGAAGTACCATACATTTGTACAGAAGGA

GAAGACCAAATTACCGTTTGGGGGTTCCACTCTGACAACGAGACCC

AAATGGCAAAGCTCTATGGGGACTCAAAGCCCCAGAAGTTCACCTC

ATCTGCCAACGGAGTGACCACACATTACGTTTCACAGATTGGTGGC

TTCCCAAATCAAACAGAAGACGGAGGACTACCACAAAGTGGCAGAA

TTGTTGTTGATTACATGGTGCAGAAATCTGGAAAAACAGGAACAATT

ACCTATCAAAGAGGTATTTTATTGCCTCAAAAAGTGTGGTGCGCAAG

TGGCAGGAGCAAGGTAATAAAAGGATCCTTGCCCTTAATTGGAGAA

GCAGATTGCCTCCATGAAAAATACGGTGGATTAAACAAAAGCAAGC

CTTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAAT

ATGGGTGAAAACACCCTTGAAGCTGGCCAATGGAACCAAATATAGA

CCCCCTGCAAAACTATTAAAGGAAAGAGGTTTCTTCGGAGCCATTGC

TGGTTTCTTAGAGGGAGGATGGGAAGGAATGATTGCAGGTTGGCAC

GGATACACATCCCATGGGGCACATGGAGTAGCGGTGGCAGCTGAC

CTTAAGAGCACTCAAGAGGCCATAAACAAGATAACAAAAAATCTCAA

CTCTTTGAGTGAGCTGGAAGTAAAGAATCTTCAAAGACTAAGCGGTG

CCATGGATGAACTCCACAACGAAATACTAGAACTAGATGAGAAAGTG

GATGATCTCAGAGCTGACACAATAAGCTCACAAATAGAACTCGCAGT

CCTGCTTTCCAATGAAGGAATAATAAACAGTGAAGATGAACATCTCT

TGGCGCTTGAAAGAAAGCTGAAGAAAATGCTGGGCCCCTCTGCTGT

AGAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCAG

ACCTGCCTCGACAGAATAGCTGCTGGTACCTTTGATGCAGGAGAAT

TTTCTCTCCCCACCTTTGATTCACTGAATATTACTGCTGCATCTTTAA

ATGACGACGGATTGGACAATCATACTATACTGCTTTACTACTCAACT

GCTGCCTCCAGTTTGGCTGTAACACTGATGATAGCTATCTTTGTTGT

TTATATGGTCTCCAGAGACAATGTTTCTTGCTCCATTTGTCTATAAGG

AAAGTTAAGCCCTGTATTTTCCTTTATTGTAGTGCTTGTTTGCTTGTT

GTCATTACAAAGAAACGTTATTGAAAAAT

In some embodiments, the portion of a coronavirus spike (S) protein and the portion of an Influenza hemagglutinin (HA) protein are operatively linked to a generic promoter. The generic promoter may be a CMV or a AG promoter.

In some embodiments, the portion of a coronavirus spike (S) protein and the portion of an influenza hemagglutinin (HA) protein are separated by a linker. The linker may be 2 to 10 amino acids in length.

In some embodiments, the compositions described herein may be used to prevent a coronavirus and/or influenza infection prophylactically in a subject. In some embodiments, the compositions described herein may elicit an immune response in a subject.

TABLE 5

and FIG. 31 and 32B shows examples of vaccine compositions described herein.

The present invention is not limited to the examples in Table 4:

Vaccine

SEQ ID

Candidate
Sequence
NO:

1

TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATAT

383

promoter
,

ATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTG

5’UTR and

ACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTC

leader

CCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAG

sequence.

TATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATG

Spike

CCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTG

glycoprotein

GCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTA

(HexaPro-

CATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCA

mutations
),

GTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAA

embedded image

GTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAAT

embedded image

CAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCA

embedded image

AATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT

GGTTTAGTGAACCGTCAGATC

GAGAATAAACTAGTATTCTTCTGGTCC

CCACAGACTCAGAGAGAACCCGCCACCATGTTCGTGTTCCTGGTGCT

GCTGCCCCTGGTGAGCAGCCAGTGCGTGAACCTGACCACCAGGACC

CAGCTGCCCCCCGCCTACACCAACAGCTTCACCAGGGGCGTGTACT

ACCCCGACAAGGTGTTCAGGAGCAGCGTGCTGCACAGCACCCAGGA

CCTGTTCCTGCCCTTCTTCAGCAACGTGACCTGGTTCCACGCCATCC

ACGTGAGCGGCACCAACGGCACCAAGAGGTTCGACAACCCCGTGCT

GCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGAGAAGAGCAAC

ATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAGCAAGACCC

AGAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGATCAAGGT

GTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTACTACC

ACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTACAG

CAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCTG

ATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGT

TCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCAC

ACCCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCC

TGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTT

CCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGAC

AGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGC

TACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCA

CCATCACCGACGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGAC

CAAGTGCACCCTGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAG

ACCAGCAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTTCC

CCAACATCACCAACCTGTGCCCCTTCGGCGAGGTGTTCAACGCCAC

CAGGTTCGCCAGCGTGTACGCCTGGAACAGGAAGAGGATCAGCAAC

TGCGTGGCCGACTACAGCGTGCTGTACAACAGCGCCAGCTTCAGCA

CCTTCAAGTGCTACGGCGTGAGCCCCACCAAGCTGAACGACCTGTG

CTTCACCAACGTGTACGCCGACAGCTTCGTGATCAGGGGCGACGAG

GTGAGGCAGATCGCCCCCGGCCAGACCGGCAAGATCGCCGACTACA

ACTACAAGCTGCCCGACGACTTCACCGGCTGCGTGATCGCCTGGAA

CAGCAACAACCTGGACAGCAAGGTGGGCGGCAACTACAACTACCTG

TACAGGCTGTTCAGGAAGAGCAACCTGAAGCCCTTCGAGAGGGACA

TCAGCACCGAGATCTACCAGGCCGGCAGCACCCCCTGCAACGGCGT

GGAGGGCTTCAACTGCTACTTCCCCCTGCAGAGCTACGGCTTCCAG

CCCACCAACGGCGTGGGCTACCAGCCCTACAGGGTGGTGGTGCTGA

GCTTCGAGCTGCTGCACGCCCCCGCCACCGTGTGCGGCCCCAAGAA

GAGCACCAACCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACG

GCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACAAGAAGTTCCT

GCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCACCGACGCC

GTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACCCCCTGCA

GCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACACCAGCAA

CCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGGTGCCC

GTGGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTGTACA

GCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGATCG

GCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCGG

CGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCGGC

AGCGCCAGC
AGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGA

GCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGC

CATCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCG

TGAGCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGG

CGACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTC

TGCACCCAGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAG

GACAAGAACACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACA

AGACCCCCCCCATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATC

CTGCCCGACCCCAGCAAGCCCAGCAAGAGGAGCCCCATCGAGGAC

CTGCTGTTCAACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGC

AGTACGGCGACTGCCTGGGCGACATCGCCGCCAGGGACCTGATCTG

CGCCCAGAAGTTCAACGGCCTGACCGTGCTGCCCCCCCTGCTGACC

GACGAGATGATCGCCCAGTACACCAGCGCCCTGCTGGCCGGCACCA

TCACCAGCGGCTGGACCTTCGGCGCCGGCCCCGCCCTGCAGATCC

CCTTCCCCATGCAGATGGCCTACAGGTTCAACGGCATCGGCGTGAC

CCAGAACGTGCTGTACGAGAACCAGAAGCTGATCGCCAACCAGTTCA

ACAGCGCCATCGGCAAGATCCAGGACAGCCTGAGCAGCACCCCCAG

CGCCCTGGGCAAGCTGCAGGACGTGGTGAACCAGAACGCCCAGGC

CCTGAACACCCTGGTGAAGCAGCTGAGCAGCAACTTCGGCGCCATC

AGCAGCGTGCTGAACGACATCCTGAGCAGGCTGGACCCCCCCGAGG

CCGAGGTGCAGATCGACAGGCTGATCACCGGCAGGCTGCAGAGCCT

GCAGACCTACGTGACCCAGCAGCTGATCAGGGCCGCCGAGATCAGG

GCCAGCGCCAACCTGGCCGCCACCAAGATGAGCGAGTGCGTGCTG

GGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGGGCTACCACCTGA

TGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTGTTCCTGCACGT

GACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACCGCCCCCGCC

ATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGGCGTGTTCG

TGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTTCTACGA

GCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAACTGC

GACGTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCTGC

AGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATC

AACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACG

AGGTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCT

GGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTG

GGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCT

GTGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGC

TGCGGCAGCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTG

CTGAAGGGCGTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACT

TCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCT

embedded image

2

TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATAT

390

promoter
,

ATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTG

5’UTR and

ACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTC

leader

CCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAG

sequence.

TATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATG

Spike

CCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTG

glycoprotein

GCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTA

(two proline

CATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCA

substitutions
),

GTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAA

embedded image

GTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAAT

embedded image

CAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCA

embedded image

AATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT

GGTTTAGTGAACCGTCAGATC

GAGAATAAACTAGTATTCTTCcTGGTC

CCCACAGACTCAGAGAGAACCCGCCACCATGTTCGTGTTCCTGGTG

CTGCTGCCCCTGGTGAGCAGCCAGTGCGTGAACCTGACCACCAGGA

CCCAGCTGCCCCCCGCCTACACCAACAGCTTCACCAGGGGCGTGTA

CTACCCCGACAAGGTGTTCAGGAGCAGCGTGCTGCACAGCACCCAG

GACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTGGTTCCACGCCAT

CCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGACAACCCCGTG

CTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGAGAAGAGCA

ACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAGCAAGAC

CCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGATCAAG

GTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTACTA

CCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTAC

AGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCC

TGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGA

GTTCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGC

ACACCCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGC

CCTGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAGG

TTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCG

ACAGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGG

GCTACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGG

CACCATCACCGACGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAG

ACCAAGTGCACCCTGAAGAGCTTCACCGTGGAGAAGGGCATCTACC

AGACCAGCAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTT

CCCCAACATCACCAACCTGTGCCCCTTCGGCGAGGTGTTCAACGCC

ACCAGGTTCGCCAGCGTGTACGCCTGGAACAGGAAGAGGATCAGCA

ACTGCGTGGCCGACTACAGCGTGCTGTACAACAGCGCCAGCTTCAG

CACCTTCAAGTGCTACGGCGTGAGCCCCACCAAGCTGAACGACCTG

TGCTTCACCAACGTGTACGCCGACAGCTTCGTGATCAGGGGCGACG

AGGTGAGGCAGATCGCCCCCGGCCAGACCGGCAAGATCGCCGACT

ACAACTACAAGCTGCCCGACGACTTCACCGGCTGCGTGATCGCCTG

GAACAGCAACAACCTGGACAGCAAGGTGGGCGGCAACTACAACTAC

CTGTACAGGCTGTTCAGGAAGAGCAACCTGAAGCCCTTCGAGAGGG

ACATCAGCACCGAGATCTACCAGGCCGGCAGCACCCCCTGCAACGG

CGTGGAGGGCTTCAACTGCTACTTCCCCCTGCAGAGCTACGGCTTC

CAGCCCACCAACGGCGTGGGCTACCAGCCCTACAGGGTGGTGGTG

CTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGTGCGGCCCCA

AGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACTTCAACTTC

AACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACAAGAAGT

TCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCACCGA

CGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACCCCC

TGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACACCA

GCAACCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGGT

GCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGT

GTACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTG

ATCGGCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCA

TCGGCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCCC

CAGGAGGGCCAGGAGCGTGGCCAGCCAGAGCATCATCGCCTACAC

CATGAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGC

ATCGCCATCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCT

GCCCGTGAGCATGACCAAGACCAGCGTGGACTGCACCATGTACATC

TGCGGCGACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCA

GCTTCTGCACCCAGCTGAACAGGGCCCTGACCGGCATCGCCGTGGA

GCAGGACAAGAACACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATC

TACAAGACCCCCCCCATCAAGGACTTCGGCGGCTTCAACTTCAGCCA

GATCCTGCCCGACCCCAGCAAGCCCAGCAAGAGGAGCTTCATCGAG

GACCTGCTGTTCAACAAGGTGACCCTGGCCGACGCCGGCTTCATCA

AGCAGTACGGCGACTGCCTGGGCGACATCGCCGCCAGGGACCTGAT

CTGCGCCCAGAAGTTCAACGGCCTGACCGTGCTGCCCCCCCTGCTG

ACCGACGAGATGATCGCCCAGTACACCAGCGCCCTGCTGGCCGGCA

CCATCACCAGCGGCTGGACCTTCGGCGCCGGCGCCGCCCTGCAGA

TCCCCTTCGCCATGCAGATGGCCTACAGGTTCAACGGCATCGGCGT

GACCCAGAACGTGCTGTACGAGAACCAGAAGCTGATCGCCAACCAG

TTCAACAGCGCCATCGGCAAGATCCAGGACAGCCTGAGCAGCACCG

CCAGCGCCCTGGGCAAGCTGCAGGACGTGGTGAACCAGAACGCCC

AGGCCCTGAACACCCTGGTGAAGCAGCTGAGCAGCAACTTCGGCGC

CATCAGCAGCGTGCTGAACGACATCCTGAGCAGGCTGGACCCCCCC

GAGGCCGAGGTGCAGATCGACAGGCTGATCACCGGCAGGCTGCAG

AGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGGGCCGCCGAGA

TCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAGCGAGTGCGT

GCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGGGCTACCA

CCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTGTTCCTG

CACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACCGCCC

CCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGGCG

TGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTT

CTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGC

AACTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTACGACC

CCCTGCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTA

CTTCAAGAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGC

GGCATCAACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGC

TGAACGAGGTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCA

GGAGCTGGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATC

TGGCTGGGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTGACCA

TCATGCTGTGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTG

CTGCAGCTGCGGCAGCTGCTGCAAGTTCGACGAGGACGACAGCGAG

CCCGTGCTGAAGGGCGTGAAGCTGCACTACACCGGAAGCGGAGCCA

CGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCC

embedded image

3

CTCGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGT

391

promoter
,

CATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGG

5’UTR and

TAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGAC

leader

GTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCA

sequence.

TTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAG

Spike

TACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATG

glycoprotein

ACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGG

(HexaPro-

GACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACC

mutations
),

ATGGTCGAGGTGAGCCCCACGTTCTGCTTCACTCTCCCCATCTCCCC

embedded image

CCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTTTGT

embedded image

GCAGCGATGGGGGCGGGGGGGGGGGGGGGGCGCGCGCCAGGCG

embedded image

GGGCGGGGCGGGGCGAGGGGCGGGGCGGGGCGAGGCGGAGAGG

TGCGGCGGCAGCCAATCAGAGCGGCGCGCTCCGAAAGTTTCCTTTT

ATGGCGAGGCGGCGGCGGCGGCGGCCCTATAAAAAGCGAAGCGCG

CGGCGGGCGGGAGTCGCTGCGCGCTGCCTTCGCCCCGTGCCCCGC

TCCGCCGCCGCCTCGCGCCGCCCGCCCCGGCTCTGACTGACCGCG

TTACTCCCACAGGTGAGCGGGCGGGACGGCCCTTCTCCTCCGGGCT

GTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGC

GTGAAAGCCTTGAGGGGCTCCGGGAGGGCCCTTTGTGCGGGGGGA

GCGGCTCGGGGGGTGCGTGCGTGTGTGTGTGCGTGGGGAGCGCCG

CGTGCGGCTCCGCGCTGCCCGGCGGCTGTGAGCGCTGCGGGCGCG

GCGCGGGGCTTTGTGCGCTCCGCAGTGTGCGCGAGGGGAGCGCGG

CCGGGGGCGGTGCCCCGCGGTGCGGGGGGGGCTGCGAGGGGAAC

AAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGGGTGAGCAGGGGGT

GTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTGCACCCCCCTCCC

CGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGGGCTCCGTACG

GGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGGTGGCGGCA

GGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCCGGGGAG

GGCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGCGGCTG

TCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCGTGC

GAGAGGGCGCAGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCGA

AATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGA

AGCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTC

GTGCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGG

CTGTCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGG

CGGGGTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGC

TAACCATGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGT

GCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAGAATTG

GAGAATAA

ACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGAACCCGCCACCA

TGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGT

GAACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAGC

TTCACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCG

TGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTG

ACCTGGTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAGA

GGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGC

CAGCACCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACC

ACCCTGGACAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCA

CCAACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCC

CTTCCTGGGCGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGA

GCGAGTTCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTA

CGTGAGCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAAC

TTCAAGAACCTGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTT

CAAGATCTACAGCAAGCACACCCCCATCAACCTGGTGAGGGACCTG

CCCCAGGGCTTCAGCGCCCTGGAGCCCCTGGTGGACCTGCCCATCG

GCATCAACATCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAG

CTACCTGACCCCCGGCGACAGCAGCAGCGGCTGGACCGCCGGCGC

CGCCGCCTACTACGTGGGCTACCTGCAGCCCAGGACCTTCCTGCTG

AAGTACAACGAGAACGGCACCATCACCGACGCCGTGGACTGCGCCC

TGGACCCCCTGAGCGAGACCAAGTGCACCCTGAAGAGCTTCACCGT

GGAGAAGGGCATCTACCAGACCAGCAACTTCAGGGTGCAGCCCACC

GAGAGCATCGTGAGGTTCCCCAACATCACCAACCTGTGCCCCTTCG

GCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGTACGCCTGGAA

CAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTAC

AACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTGAGCCCCA

CCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTC

GTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCAGACC

GGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCACCG

GCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGGG

CGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAG

CACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTG

CAGAGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCT

ACAGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCAC

CGTGTGCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGC

GTGAACTTCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCG

AGAGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACAT

CGCCGACACCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGAT

CCTGGACATCACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACC

CCCGGCACCAACACCAGCAACCAGGTGGCCGTGCTGTACCAGGACG

TGAACTGCACCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGAC

CCCCACCTGGAGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACC

AGGGCCGGCTGCCTGATCGGCGCCGAGCACGTGAACAACAGCTAC

GAGTGCGACATCCCCATCGGCGCCGGCATCTGCGCCAGCTACCAGA

CCCAGACCAACAGCCCCGGCAGCGCCAGCAGCGTGGCCAGCCAGA

GCATCATCGCCTACACCATGAGCCTGGGCGCCGAGAACAGCGTGGC

CTACAGCAACAACAGCATCGCCATCCCCACCAACTTCACCATCAGCG

TGACCACCGAGATCCTGCCCGTGAGCATGACCAAGACCAGCGTGGA

CTGCACCATGTACATCTGCGGCGACAGCACCGAGTGCAGCAACCTG

CTGCTGCAGTACGGCAGCTTCTGCACCCAGCTGAACAGGGCCCTGA

CCGGCATCGCCGTGGAGCAGGACAAGAACACCCAGGAGGTGTTCGC

CCAGGTGAAGCAGATCTACAAGACCCCCCCCATCAAGGACTTCGGC

GGCTTCAACTTCAGCCAGATCCTGCCCGACCCCAGCAAGCCCAGCA

AGAGGAGCCCCATCGAGGACCTGCTGTTCAACAAGGTGACCCTGGC

CGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCTGGGCGACATC

GCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACGGCCTGACCG

TGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAGTACACCAG

CGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTTCGGCGC

CGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATGGCCTACAGG

TTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACCAGA

AGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGGA

CAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGT

GGTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTG

AGCAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGA

GCAGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGA

TCACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCT

GATCAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCAC

CAAGATGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTC

TGCGGCAAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCC

ACGGCGTGGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAA

GAACTTCACCACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCAC

TTCCCCAGGGAGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCG

TGACCCAGAGGAACTTCTACGAGCCCCAGATCATCACCACCGACAAC

ACCTTCGTGAGCGGCAACTGCGACGTGGTGATCGGCATCGTGAACA

ACACCGTGTACGACCCCCTGCAGCCCGAGCTGGACAGCTTCAAGGA

GGAGCTGGACAAGTACTTCAAGAACCACACCAGCCCCGACGTGGAC

CTGGGCGACATCAGCGGCATCAACGCCAGCGTGGTGAACATCCAGA

AGGAGATCGACAGGCTGAACGAGGTGGCCAAGAACCTGAACGAGAG

CCTGATCGACCTGCAGGAGCTGGGCAAGTACGAGCAGTACATCAAG

TGGCCCTGGTACATCTGGCTGGGCTTCATCGCCGGCCTGATCGCCA

TCGTGATGGTGACCATCATGCTGTGCTGCATGACCAGCTGCTGCAGC

TGCCTGAAGGGCTGCTGCAGCTGCGGCAGCTGCTGCAAGTTCGACG

AGGACGACAGCGAGCCCGTGCTGAAGGGCGTGAAGCTGCACTACAC

CGGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGAT

embedded image

4

CTCGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGT

392

promoter,

CATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGG

5’UTR and

TAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGAC

leader

GTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCA

sequence.

TTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAG

Spike

TACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATG

glycoprotein

ACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGG

(two proline

GACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACC

substitutions
),

ATGGTCGAGGTGAGCCCCACGTTCTGCTTCACTCTCCCCATCTCCCC

embedded image

CCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTTTGT

embedded image

GCAGCGATGGGGGCGGGGGGGGGGGGGGGGCGCGCGCCAGGCG

embedded image

GGGCGGGGCGGGGCGAGGGGCGGGGCGGGGCGAGGCGGAGAGG

TGCGGCGGCAGCCAATCAGAGCGGCGCGCTCCGAAAGTTTCCTTTT

ATGGCGAGGCGGCGGCGGCGGCGGCCCTATAAAAAGCGAAGCGCG

CGGCGGGCGGGAGTCGCTGCGCGCTGCCTTCGCCCCGTGCCCCGC

TCCGCCGCCGCCTCGCGCCGCCCGCCCCGGCTCTGACTGACCGCG

TTACTCCCACAGGTGAGCGGGCGGGACGGCCCTTCTCCTCCGGGCT

GTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGC

GTGAAAGCCTTGAGGGGCTCCGGGAGGGCCCTTTGTGCGGGGGGA

GCGGCTCGGGGGGTGCGTGCGTGTGTGTGTGCGTGGGGAGCGCCG

CGTGCGGCTCCGCGCTGCCCGGCGGCTGTGAGCGCTGCGGGCGCG

GCGCGGGGCTTTGTGCGCTCCGCAGTGTGCGCGAGGGGAGCGCGG

CCGGGGGCGGTGCCCCGCGGTGCGGGGGGGGCTGCGAGGGGAAC

AAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGGGTGAGCAGGGGGT

GTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTGCACCCCCCTCCC

CGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGGGCTCCGTACG

GGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGGTGGCGGCA

GGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCCGGGGAG

GGCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGCGGCTG

TCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCGTGC

GAGAGGGCGCAGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCGA

AATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGA

AGCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTC

GTGCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGG

CTGTCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGG

CGGGGTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGC

TAACCATGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGT

GCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAGAATTG

GAGAATAA

ACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGAACCCGCCACCA

TGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGT

GAACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAGC

TTCACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCG

TGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTG

ACCTGGTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAGA

GGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGC

CAGCACCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACC

ACCCTGGACAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCA

CCAACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCC

CTTCCTGGGCGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGA

GCGAGTTCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTA

CGTGAGCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAAC

TTCAAGAACCTGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTT

CAAGATCTACAGCAAGCACACCCCCATCAACCTGGTGAGGGACCTG

CCCCAGGGCTTCAGCGCCCTGGAGCCCCTGGTGGACCTGCCCATCG

GCATCAACATCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAG

CTACCTGACCCCCGGCGACAGCAGCAGCGGCTGGACCGCCGGCGC

CGCCGCCTACTACGTGGGCTACCTGCAGCCCAGGACCTTCCTGCTG

AAGTACAACGAGAACGGCACCATCACCGACGCCGTGGACTGCGCCC

TGGACCCCCTGAGCGAGACCAAGTGCACCCTGAAGAGCTTCACCGT

GGAGAAGGGCATCTACCAGACCAGCAACTTCAGGGTGCAGCCCACC

GAGAGCATCGTGAGGTTCCCCAACATCACCAACCTGTGCCCCTTCG

GCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGTACGCCTGGAA

CAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTAC

AACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTGAGCCCCA

CCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTC

GTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCAGACC

GGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCACCG

GCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGGG

CGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAG

CACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTG

CAGAGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCT

ACAGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCAC

CGTGTGCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGC

GTGAACTTCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCG

AGAGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACAT

CGCCGACACCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGAT

CCTGGACATCACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACC

CCCGGCACCAACACCAGCAACCAGGTGGCCGTGCTGTACCAGGACG

TGAACTGCACCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGAC

CCCCACCTGGAGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACC

AGGGCCGGCTGCCTGATCGGCGCCGAGCACGTGAACAACAGCTAC

GAGTGCGACATCCCCATCGGCGCCGGCATCTGCGCCAGCTACCAGA

CCCAGACCAACAGCCCCAGGAGGGCCAGGAGCGTGGCCAGCCAGA

GCATCATCGCCTACACCATGAGCCTGGGCGCCGAGAACAGCGTGGC

CTACAGCAACAACAGCATCGCCATCCCCACCAACTTCACCATCAGCG

TGACCACCGAGATCCTGCCCGTGAGCATGACCAAGACCAGCGTGGA

CTGCACCATGTACATCTGCGGCGACAGCACCGAGTGCAGCAACCTG

CTGCTGCAGTACGGCAGCTTCTGCACCCAGCTGAACAGGGCCCTGA

CCGGCATCGCCGTGGAGCAGGACAAGAACACCCAGGAGGTGTTCGC

CCAGGTGAAGCAGATCTACAAGACCCCCCCCATCAAGGACTTCGGC

GGCTTCAACTTCAGCCAGATCCTGCCCGACCCCAGCAAGCCCAGCA

AGAGGAGCTTCATCGAGGACCTGCTGTTCAACAAGGTGACCCTGGC

CGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCTGGGCGACATC

GCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACGGCCTGACCG

TGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAGTACACCAG

CGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTTCGGCGC

CGGCGCCGCCCTGCAGATCCCCTTCGCCATGCAGATGGCCTACAGG

TTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACCAGA

AGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGGA

CAGCCTGAGCAGCACCGCCAGCGCCCTGGGCAAGCTGCAGGACGT

GGTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTG

AGCAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGA

GCAGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGA

TCACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCT

GATCAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCAC

CAAGATGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTC

TGCGGCAAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCC

ACGGCGTGGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAA

GAACTTCACCACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCAC

TTCCCCAGGGAGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCG

TGACCCAGAGGAACTTCTACGAGCCCCAGATCATCACCACCGACAAC

ACCTTCGTGAGCGGCAACTGCGACGTGGTGATCGGCATCGTGAACA

ACACCGTGTACGACCCCCTGCAGCCCGAGCTGGACAGCTTCAAGGA

GGAGCTGGACAAGTACTTCAAGAACCACACCAGCCCCGACGTGGAC

CTGGGCGACATCAGCGGCATCAACGCCAGCGTGGTGAACATCCAGA

AGGAGATCGACAGGCTGAACGAGGTGGCCAAGAACCTGAACGAGAG

CCTGATCGACCTGCAGGAGCTGGGCAAGTACGAGCAGTACATCAAG

TGGCCCTGGTACATCTGGCTGGGCTTCATCGCCGGCCTGATCGCCA

TCGTGATGGTGACCATCATGCTGTGCTGCATGACCAGCTGCTGCAGC

TGCCTGAAGGGCTGCTGCAGCTGCGGCAGCTGCTGCAAGTTCGACG

AGGACGACAGCGAGCCCGTGCTGAAGGGCGTGAAGCTGCACTACAC

CGGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGAT

embedded image

5

ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCG

393

leader

TGAACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAG

sequence,
CTTCACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGC

Spike
GTGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGT

glycoprotein
GACCTGGTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAG

(HexaPro-
AGGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCG

mutations
),
CCAGCACCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCAC

embedded image

CACCCTGGACAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCC

embedded image

ACCAACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCC

CTTCCTGGGCGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGA

GCGAGTTCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTA

CGTGAGCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAAC

TTCAAGAACCTGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTT

CAAGATCTACAGCAAGCACACCCCCATCAACCTGGTGAGGGACCTG

CCCCAGGGCTTCAGCGCCCTGGAGCCCCTGGTGGACCTGCCCATCG

GCATCAACATCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAG

CTACCTGACCCCCGGCGACAGCAGCAGCGGCTGGACCGCCGGCGC

CGCCGCCTACTACGTGGGCTACCTGCAGCCCAGGACCTTCCTGCTG

AAGTACAACGAGAACGGCACCATCACCGACGCCGTGGACTGCGCCC

TGGACCCCCTGAGCGAGACCAAGTGCACCCTGAAGAGCTTCACCGT

GGAGAAGGGCATCTACCAGACCAGCAACTTCAGGGTGCAGCCCACC

GAGAGCATCGTGAGGTTCCCCAACATCACCAACCTGTGCCCCTTCG

GCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGTACGCCTGGAA

CAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTAC

AACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTGAGCCCCA

CCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTC

GTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCAGACC

GGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCACCG

GCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGGG

CGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAG

CACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTG

CAGAGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCT

ACAGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCAC

CGTGTGCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGC

GTGAACTTCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCG

AGAGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACAT

CGCCGACACCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGAT

CCTGGACATCACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACC

CCCGGCACCAACACCAGCAACCAGGTGGCCGTGCTGTACCAGGACG

TGAACTGCACCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGAC

CCCCACCTGGAGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACC

AGGGCCGGCTGCCTGATCGGCGCCGAGCACGTGAACAACAGCTAC

GAGTGCGACATCCCCATCGGCGCCGGCATCTGCGCCAGCTACCAGA

CCCAGACCAACAGCCCCGGCAGCGCCAGCAGCGTGGCCAGCCAGA

GCATCATCGCCTACACCATGAGCCTGGGCGCCGAGAACAGCGTGGC

CTACAGCAACAACAGCATCGCCATCCCCACCAACTTCACCATCAGCG

TGACCACCGAGATCCTGCCCGTGAGCATGACCAAGACCAGCGTGGA

CTGCACCATGTACATCTGCGGCGACAGCACCGAGTGCAGCAACCTG

CTGCTGCAGTACGGCAGCTTCTGCACCCAGCTGAACAGGGCCCTGA

CCGGCATCGCCGTGGAGCAGGACAAGAACACCCAGGAGGTGTTCGC

CCAGGTGAAGCAGATCTACAAGACCCCCCCCATCAAGGACTTCGGC

GGCTTCAACTTCAGCCAGATCCTGCCCGACCCCAGCAAGCCCAGCA

AGAGGAGCCCCATCGAGGACCTGCTGTTCAACAAGGTGACCCTGGC

CGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCTGGGCGACATC

GCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACGGCCTGACCG

TGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAGTACACCAG

CGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTTCGGCGC

CGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATGGCCTACAGG

TTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACCAGA

AGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGGA

CAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGT

GGTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTG

AGCAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGA

GCAGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGA

TCACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCT

GATCAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCAC

CAAGATGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTC

TGCGGCAAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCC

ACGGCGTGGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAA

GAACTTCACCACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCAC

TTCCCCAGGGAGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCG

TGACCCAGAGGAACTTCTACGAGCCCCAGATCATCACCACCGACAAC

ACCTTCGTGAGCGGCAACTGCGACGTGGTGATCGGCATCGTGAACA

ACACCGTGTACGACCCCCTGCAGCCCGAGCTGGACAGCTTCAAGGA

GGAGCTGGACAAGTACTTCAAGAACCACACCAGCCCCGACGTGGAC

CTGGGCGACATCAGCGGCATCAACGCCAGCGTGGTGAACATCCAGA

AGGAGATCGACAGGCTGAACGAGGTGGCCAAGAACCTGAACGAGAG

CCTGATCGACCTGCAGGAGCTGGGCAAGTACGAGCAGTACATCAAG

TGGCCCTGGTACATCTGGCTGGGCTTCATCGCCGGCCTGATCGCCA

TCGTGATGGTGACCATCATGCTGTGCTGCATGACCAGCTGCTGCAGC

TGCCTGAAGGGCTGCTGCAGCTGCGGCAGCTGCTGCAAGTTCGACG

AGGACGACAGCGAGCCCGTGCTGAAGGGCGTGAAGCTGCACTACAC

CGGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGAT

embedded image

6

ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCG

394

leader

TGAACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAG

sequence,
CTTCACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGC

Spike
GTGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGT

glycoprotein
GACCTGGTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAG

(two proline
AGGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCG

substitutions
)
CCAGCACCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCAC

embedded image

CACCCTGGACAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCC

embedded image

ACCAACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCC

CTTCCTGGGCGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGA

GCGAGTTCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTA

CGTGAGCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAAC

TTCAAGAACCTGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTT

CAAGATCTACAGCAAGCACACCCCCATCAACCTGGTGAGGGACCTG

CCCCAGGGCTTCAGCGCCCTGGAGCCCCTGGTGGACCTGCCCATCG

GCATCAACATCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAG

CTACCTGACCCCCGGCGACAGCAGCAGCGGCTGGACCGCCGGCGC

CGCCGCCTACTACGTGGGCTACCTGCAGCCCAGGACCTTCCTGCTG

AAGTACAACGAGAACGGCACCATCACCGACGCCGTGGACTGCGCCC

TGGACCCCCTGAGCGAGACCAAGTGCACCCTGAAGAGCTTCACCGT

GGAGAAGGGCATCTACCAGACCAGCAACTTCAGGGTGCAGCCCACC

GAGAGCATCGTGAGGTTCCCCAACATCACCAACCTGTGCCCCTTCG

GCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGTACGCCTGGAA

CAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTAC

AACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTGAGCCCCA

CCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTC

GTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCAGACC

GGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCACCG

GCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGGG

CGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAG

CACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTG

CAGAGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCT

ACAGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCAC

CGTGTGCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGC

GTGAACTTCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCG

AGAGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACAT

CGCCGACACCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGAT

CCTGGACATCACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACC

CCCGGCACCAACACCAGCAACCAGGTGGCCGTGCTGTACCAGGACG

TGAACTGCACCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGAC

CCCCACCTGGAGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACC

AGGGCCGGCTGCCTGATCGGCGCCGAGCACGTGAACAACAGCTAC

GAGTGCGACATCCCCATCGGCGCCGGCATCTGCGCCAGCTACCAGA

CCCAGACCAACAGCCCCAGGAGGGCCAGGAGCGTGGCCAGCCAGA

GCATCATCGCCTACACCATGAGCCTGGGCGCCGAGAACAGCGTGGC

CTACAGCAACAACAGCATCGCCATCCCCACCAACTTCACCATCAGCG

TGACCACCGAGATCCTGCCCGTGAGCATGACCAAGACCAGCGTGGA

CTGCACCATGTACATCTGCGGCGACAGCACCGAGTGCAGCAACCTG

CTGCTGCAGTACGGCAGCTTCTGCACCCAGCTGAACAGGGCCCTGA

CCGGCATCGCCGTGGAGCAGGACAAGAACACCCAGGAGGTGTTCGC

CCAGGTGAAGCAGATCTACAAGACCCCCCCCATCAAGGACTTCGGC

GGCTTCAACTTCAGCCAGATCCTGCCCGACCCCAGCAAGCCCAGCA

AGAGGAGCTTCATCGAGGACCTGCTGTTCAACAAGGTGACCCTGGC

CGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCTGGGCGACATC

GCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACGGCCTGACCG

TGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAGTACACCAG

CGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTTCGGCGC

CGGCGCCGCCCTGCAGATCCCCTTCGCCATGCAGATGGCCTACAGG

TTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACCAGA

AGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGGA

CAGCCTGAGCAGCACCGCCAGCGCCCTGGGCAAGCTGCAGGACGT

GGTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTG

AGCAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGA

GCAGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGA

TCACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCT

GATCAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCAC

CAAGATGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTC

TGCGGCAAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCC

ACGGCGTGGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAA

GAACTTCACCACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCAC

TTCCCCAGGGAGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCG

TGACCCAGAGGAACTTCTACGAGCCCCAGATCATCACCACCGACAAC

ACCTTCGTGAGCGGCAACTGCGACGTGGTGATCGGCATCGTGAACA

ACACCGTGTACGACCCCCTGCAGCCCGAGCTGGACAGCTTCAAGGA

GGAGCTGGACAAGTACTTCAAGAACCACACCAGCCCCGACGTGGAC

CTGGGCGACATCAGCGGCATCAACGCCAGCGTGGTGAACATCCAGA

AGGAGATCGACAGGCTGAACGAGGTGGCCAAGAACCTGAACGAGAG

CCTGATCGACCTGCAGGAGCTGGGCAAGTACGAGCAGTACATCAAG

TGGCCCTGGTACATCTGGCTGGGCTTCATCGCCGGCCTGATCGCCA

TCGTGATGGTGACCATCATGCTGTGCTGCATGACCAGCTGCTGCAGC

TGCCTGAAGGGCTGCTGCAGCTGCGGCAGCTGCTGCAAGTTCGACG

AGGACGACAGCGAGCCCGTGCTGAAGGGCGTGAAGCTGCACTACAC

CGGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGAT

embedded image

Pan-CoV-Flu

TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATAT

395

Vaccine #7

ATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTG

CoV- Omicron

ACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTC

BA.2

CCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAG

Spike(HexaPro)-

TATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATG

Influenza (HA)

CCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTG

GCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTA

CATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCA

GTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAA

GTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAAT

CAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCA

AATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT

GGTTTAGTGAACCGTCAGATC

GAGAATAAACTAGTATTCTTCTGGTCC

CCACAGACTCAGAGAGAACCCGCCACCATGTTCGTGTTCCTGGTGCT

GCTGCCCCTGGTGAGCAGCCAGTGCGTGAACCTGATCACCAGGACC

CAGAGCTACACCAACAGCTTCACCAGGGGCGTGTACTACCCCGACA

AGGTGTTCAGGAGCAGCGTGCTGCACAGCACCCAGGACCTGTTCCT

GCCCTTCTTCAGCAACGTGACCTGGTTCCACGCCATCCACGTGAGC

GGCACCAACGGCACCAAGAGGTTCGACAACCCCGTGCTGCCCTTCA

ACGACGGCGTGTACTTCGCCAGCACCGAGAAGAGCAACATCATCAG

GGGCTGGATCTTCGGCACCACCCTGGACAGCAAGACCCAGAGCCTG

CTGATCGTGAACAACGCCACCAACGTGGTGATCAAGGTGTGCGAGTT

CCAGTTCTGCAACGACCCCTTCCTGGACGTGTACTACCACAAGAACA

ACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTACAGCAGCGCCAA

CAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCTGATGGACCTG

GAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGTTCGTGTTCA

AGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACACCCCCATC

AACCTGGGCAGGGACCTGCCCCAGGGCTTCAGCGCCCTGGAGCCC

CTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGACCCT

GCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGCAG

CGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCA

GCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACC

GACGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGC

ACCCTGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAGCA

ACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACAT

CACCAACCTGTGCCCCTTCGACGAGGTGTTCAACGCCACCAGGTTC

GCCAGCGTGTACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGG

CCGACTACAGCGTGCTGTACAACTTCGCCCCCTTCTTCGCCTTCAAG

TGCTACGGCGTGAGCCCCACCAAGCTGAACGACCTGTGCTTCACCA

ACGTGTACGCCGACAGCTTCGTGATCAGGGGCAACGAGGTGAGCCA

GATCGCCCCCGGCCAGACCGGCAACATCGCCGACTACAACTACAAG

CTGCCCGACGACTTCACCGGCTGCGTGATCGCCTGGAACAGCAACA

AGCTGGACAGCAAGGTGGGCGGCAACTACAACTACCTGTACAGGCT

GTTCAGGAAGAGCAACCTGAAGCCCTTCGAGAGGGACATCAGCACC

GAGATCTACCAGGCCGGCAACAAGCCCTGCAACGGCGTGGCCGGCT

TCAACTGCTACTTCCCCCTGAGGAGCTACGGCTTCAGGCCCACCTAC

GGCGTGGGCCACCAGCCCTACAGGGTGGTGGTGCTGAGCTTCGAG

CTGCTGCACGCCCCCGCCACCGTGTGCGGCCCCAAGAAGAGCACCA

ACCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGCCTGACC

GGCACCGGCGTGCTGACCGAGAGCAACAAGAAGTTCCTGCCCTTCC

AGCAGTTCGGCAGGGACATCGCCGACACCACCGACGCCGTGAGGG

ACCCCCAGACCCTGGAGATCCTGGACATCACCCCCTGCAGCTTCGG

CGGCGTGAGCGTGATCACCCCCGGCACCAACACCAGCAACCAGGTG

GCCGTGCTGTACCAGGGCGTGAACTGCACCGAGGTGCCCGTGGCC

ATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTGTACAGCACCG

GCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGATCGGCGCCG

AGTACGTGAACAACAGCTACGAGTGCGACATCCCCATCGGCGCCGG

CATCTGCGCCAGCTACCAGACCCAGACCAAGAGCCACCAGCAGGCC

CAGAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAGCCTGG

GCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCATCCC

CACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGAGC

ATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACA

GCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCAC

CCAGCTGAAGAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACAA

GAACACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACC

CCCCCCATCAAGTACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCC

CGACCCCAGCAAGCCCAGCAAGAGGAGCCCCATCGAGGACCTGCTG

TTCAACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACG

GCGACTGCCTGGGCGACATCGCCGCCAGGGACCTGATCTGCGCCC

AGAAGTTCAACGGCCTGACCGTGCTGCCCCCCCTGCTGACCGACGA

GATGATCGCCCAGTACACCAGCGCCCTGCTGGCCGGCACCATCACC

AGCGGCTGGACCTTCGGCGCCGGCCCCGCCCTGCAGATCCCCTTCC

CCATGCAGATGGCCTACAGGTTCAACGGCATCGGCGTGACCCAGAA

CGTGCTGTACGAGAACCAGAAGCTGATCGCCAACCAGTTCAACAGC

GCCATCGGCAAGATCCAGGACAGCCTGAGCAGCACCCCCAGCGCCC

TGGGCAAGCTGCAGGACGTGGTGAACCACAACGCCCAGGCCCTGAA

CACCCTGGTGAAGCAGCTGAGCAGCAAGTTCGGCGCCATCAGCAGC

GTGCTGAACGACATCCTGAGCAGGCTGGACCCCCCCGAGGCCGAG

GTGCAGATCGACAGGCTGATCACCGGCAGGCTGCAGAGCCTGCAGA

CCTACGTGACCCAGCAGCTGATCAGGGCCGCCGAGATCAGGGCCAG

CGCCAACCTGGCCGCCACCAAGATGAGCGAGTGCGTGCTGGGCCA

GAGCAAGAGGGTGGACTTCTGCGGCAAGGGCTACCACCTGATGAGC

TTCCCCCAGAGCGCCCCCCACGGCGTGGTGTTCCTGCACGTGACCT

ACGTGCCCGCCCAGGAGAAGAACTTCACCACCGCCCCCGCCATCTG

CCACGACGGCAAGGCCCACTTCCCCAGGGAGGGCGTGTTCGTGAG

CAACGGCACCCACTGGTTCGTGACCCAGAGGAACTTCTACGAGCCC

CAGATCATCACCACCGACAACACCTTCGTGAGCGGCAACTGCGACG

TGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCTGCAGCC

CGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAAGAAC

CACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCAACG

CCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAGGT

GGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGGC

AAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTT

CATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCT

GCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGG

CAGCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAG

GGCGTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTC

embedded image

ACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGG

CCTTTCGGAGCTATTGCTGCTTTCTTGGAAGGAGGATGGGAAGGAAT

GATTGCAGGTTGGCACGGATACACATCTCATGGAGCACATGGAGTAG

CAGTGGCAGCAGACCTTAAGAGTACCCATGATGACTCGAGCTGGTAC

TGCATGCACGCAATGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCC

GAGTCTCCCCCGACCTCGGGTCCCAGGTATGCTCCCACCTCCACCT

GCCCCACTCACCACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCA

GCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAA

CAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATA

CTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACACCCTGGAGCTA

GCAAAAAAAA

Pan-CoV-Flu
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATAT
396

Vaccine #8
ATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTG

CoV-Omicron
ACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTC

BA.2 Spike(2
CCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAG

proline
TATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATG

substitution)-
CCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTG

Influenza (HA)
GCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTA

CATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCA

GTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAA

GTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAAT

CAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCA

AATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT

GGTTTAGTGAACCGTCAGATCGAGAATAAACTAGTATTCTTCTGGTCC

CCACAGACTCAGAGAGAACCCGCCACCATGTTCGTGTTCCTGGTGCT

GCTGCCCCTGGTGAGCAGCCAGTGCGTGAACCTGATCACCAGGACC

CAGAGCTACACCAACAGCTTCACCAGGGGCGTGTACTACCCCGACA

AGGTGTTCAGGAGCAGCGTGCTGCACAGCACCCAGGACCTGTTCCT

GCCCTTCTTCAGCAACGTGACCTGGTTCCACGCCATCCACGTGAGC

GGCACCAACGGCACCAAGAGGTTCGACAACCCCGTGCTGCCCTTCA

ACGACGGCGTGTACTTCGCCAGCACCGAGAAGAGCAACATCATCAG

GGGCTGGATCTTCGGCACCACCCTGGACAGCAAGACCCAGAGCCTG

CTGATCGTGAACAACGCCACCAACGTGGTGATCAAGGTGTGCGAGTT

CCAGTTCTGCAACGACCCCTTCCTGGACGTGTACTACCACAAGAACA

ACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTACAGCAGCGCCAA

CAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCTGATGGACCTG

GAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGTTCGTGTTCA

AGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACACCCCCATC

AACCTGGGCAGGGACCTGCCCCAGGGCTTCAGCGCCCTGGAGCCC

CTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGACCCT

GCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGCAG

CGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCA

GCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACC

GACGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGC

ACCCTGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAGCA

ACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACAT

CACCAACCTGTGCCCCTTCGACGAGGTGTTCAACGCCACCAGGTTC

GCCAGCGTGTACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGG

CCGACTACAGCGTGCTGTACAACTTCGCCCCCTTCTTCGCCTTCAAG

TGCTACGGCGTGAGCCCCACCAAGCTGAACGACCTGTGCTTCACCA

ACGTGTACGCCGACAGCTTCGTGATCAGGGGCAACGAGGTGAGCCA

GATCGCCCCCGGCCAGACCGGCAACATCGCCGACTACAACTACAAG

CTGCCCGACGACTTCACCGGCTGCGTGATCGCCTGGAACAGCAACA

AGCTGGACAGCAAGGTGGGCGGCAACTACAACTACCTGTACAGGCT

GTTCAGGAAGAGCAACCTGAAGCCCTTCGAGAGGGACATCAGCACC

GAGATCTACCAGGCCGGCAACAAGCCCTGCAACGGCGTGGCCGGCT

TCAACTGCTACTTCCCCCTGAGGAGCTACGGCTTCAGGCCCACCTAC

GGCGTGGGCCACCAGCCCTACAGGGTGGTGGTGCTGAGCTTCGAG

CTGCTGCACGCCCCCGCCACCGTGTGCGGCCCCAAGAAGAGCACCA

ACCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGCCTGACC

GGCACCGGCGTGCTGACCGAGAGCAACAAGAAGTTCCTGCCCTTCC

AGCAGTTCGGCAGGGACATCGCCGACACCACCGACGCCGTGAGGG

ACCCCCAGACCCTGGAGATCCTGGACATCACCCCCTGCAGCTTCGG

CGGCGTGAGCGTGATCACCCCCGGCACCAACACCAGCAACCAGGTG

GCCGTGCTGTACCAGGGCGTGAACTGCACCGAGGTGCCCGTGGCC

ATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTGTACAGCACCG

GCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGATCGGCGCCG

AGTACGTGAACAACAGCTACGAGTGCGACATCCCCATCGGCGCCGG

CATCTGCGCCAGCTACCAGACCCAGACCAAGAGCCACCAGCAGGCC

CAGAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAGCCTGG

GCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCATCCC

CACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGAGC

ATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACA

GCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCAC

CCAGCTGAAGAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACAA

GAACACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACC

CCCCCCATCAAGTACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCC

CGACCCCAGCAAGCCCAGCAAGAGGAGCTTCATCGAGGACCTGCTG

TTCAACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACG

GCGACTGCCTGGGCGACATCGCCGCCAGGGACCTGATCTGCGCCC

AGAAGTTCAACGGCCTGACCGTGCTGCCCCCCCTGCTGACCGACGA

GATGATCGCCCAGTACACCAGCGCCCTGCTGGCCGGCACCATCACC

AGCGGCTGGACCTTCGGCGCCGGCGCCGCCCTGCAGATCCCCTTC

GCCATGCAGATGGCCTACAGGTTCAACGGCATCGGCGTGACCCAGA

ACGTGCTGTACGAGAACCAGAAGCTGATCGCCAACCAGTTCAACAGC

GCCATCGGCAAGATCCAGGACAGCCTGAGCAGCACCGCCAGCGCC

CTGGGCAAGCTGCAGGACGTGGTGAACCACAACGCCCAGGCCCTGA

ACACCCTGGTGAAGCAGCTGAGCAGCAAGTTCGGCGCCATCAGCAG

CGTGCTGAACGACATCCTGAGCAGGCTGGACCCCCCCGAGGCCGA

GGTGCAGATCGACAGGCTGATCACCGGCAGGCTGCAGAGCCTGCAG

ACCTACGTGACCCAGCAGCTGATCAGGGCCGCCGAGATCAGGGCCA

GCGCCAACCTGGCCGCCACCAAGATGAGCGAGTGCGTGCTGGGCC

AGAGCAAGAGGGTGGACTTCTGCGGCAAGGGCTACCACCTGATGAG

CTTCCCCCAGAGCGCCCCCCACGGCGTGGTGTTCCTGCACGTGACC

TACGTGCCCGCCCAGGAGAAGAACTTCACCACCGCCCCCGCCATCT

GCCACGACGGCAAGGCCCACTTCCCCAGGGAGGGCGTGTTCGTGA

GCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTTCTACGAGCC

CCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAACTGCGAC

GTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCTGCAGC

CCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAAGAA

CCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCAAC

GCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAGG

TGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGG

CAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGC

TTCATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTG

CTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGC

GGCAGCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGA

AGGGCGTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTC

TCTGTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTATGA

AGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAG

ACACATTATGTATAGGTTATCATGCGAACAACTCAACAGACACTGTAG

ACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTC

TAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGAGGGGTAGCC

CCATTGCATTTGGGTAAATGTAACATTGCTGGCTGGATCCTGGGAAA

TCCAGAGTGTGAATCACTCTCCACAGCAAGCTCATGGTCCTACATTG

TGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAGGAGATTTC

ATCGATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTCATCATTT

GAAAGGTTTGAGATATTCCCCAAGACAAGTTCATGGCCCAATCATGA

CTCGAACAAAGGTGTAACGACAGCATGTCCTCATGCTGGAGCAAAAA

GCTTCTACAAAAATTTAATATGGCTAGTTAAAAAAGGAAATTCATACC

CAAAGCTCAGCAAATCCTACATTAATGATAAAGGGAAAGAAGTCCTC

GTGCTATGGGGCATTCACCATCCACCTACTAGTGCTGACCAACAAAG

TCTCTATCAGAATGCAGATGCATATGTTTTTGTGGGGACATCAAGATA

CAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGGCCCAAAGTGAGG

GATCAAGAAGGGAGAATGAACTATTACTGGACACTAGTAGAGCCGGG

AGACAAAATAACATTCGAAGCAACTGGAAATCTAGTGGTACCGAGAT

ATGCATTCGCAATGGAAAGAAATGCTGGATCTGGTATTATCATTTCAG

ATACACCAGTCCACGATTGCAATACAACTTGTCAGACACCCAAGGGT

GCTATAAACACCAGCCTCCCATTTCAGAACATACATCCGATCACAATT

GGAAAATGTCCAAAATATGTAAAAAGCACAAAATTGAGACTGGCCAC

AGGATTGAGGAATGTCCCGTCCATTCAATCTAGAGGCCTATTTGGGG

CCATTGCCGGTTTCATTGAAGGGGGGTGGACAGGGATGGTAGATGG

ATGGTACGGTTATCACCATCAAAATGAGCAGGGGTCAGGATATGCAG

CCGACCTGAAGAGCACACAGAATGCCATTGACGAGATTACTAACAAA

GTAAACTCTGTTATTGAAAAAATGAATACACAGTTCACAGCAGTAGGT

AAAGAGTTCAACCACCTGGAAAAAAGAATAGAGAATTTAAATAAAAAA

GTTGATGATGGTTTCCTGGACATTTGGACTTACAATGCCGAACTGTTG

GTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAAGGTG

AAGAACTTATATGAAAAGGTAAGAAGCCAGTTAAAAAACAATGCCAAG

GAAATTGGAAACGGCTGCTTTGAATTTTACCACAAATGTGATAACACG

TGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACTCA

GAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGGTAAAGCTGGA

ATCAACAAGGATTTACCAGATTTTGGCGATCTATTCAACCGTCGCCAG

TTCATTGGTACTGGTAGTCTCCCTGGGGGCAATCAGTTTCTGGATGT

GCTCTAATGGGTCTCTACAGTGTAGAATATGTATTTAAGGAAGCGGA

GCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAAAA

CCCCGGGCCTAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGAC

TATCATTGCTTTGAGCTACATTCTATGTCTGGTTTTCGCTCAAAAACTT

CCTGGAAATGACAATAGCACTGCAACGCTGTGCCTTGGGCACCATGC

AGTACCAAACGGAACGATAGTGAAAACAATCACGAATGACCAAATTG

AAGTTACTAATGCTACTGAGCTGGTTCAGAATTCCTCAATAGGTGAAA

TATGCGACAGTCCTCATCAGATCCTTGATGGAGAAAACTGCACACTA

ATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA

GAAATGGGACCTTTTTGTTGAAGAAGCAAAGCCTACAGCAACTGTT

ACCCTTATGATGTGCCGGATTATGCCTCCCTTAGGTCACTAGTTGCC

TCATCCGGCACACTGGAGTTTAACAATGAAAGCTTCAATTGGGCTGG

AGTCACTCAAAACGGAACAAGTTCTGCTTGCATAAGGGGATCTAATA

GTAGTTTCTTTAGTAGATTAAATTGGTTGACCCACTTAAACTTCAAGTA

CCCAGCATTGAACGTGACTATGCCAAACAATGAACAATTTGACAAATT

GTACATTTGGGGGGTTCACCACCCGGGTACGGACAAGGACCAAATC

TTCCTGTATGCTCAATCATCAGGAAGAATCACAGTATCTACCAAAAGA

AGCCAACAAGCTGTAATCCCGAATATCGGATCTAGACCCAGAATAAG

GAATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAACCGG

GAGACATACTTTTGATTAACAGCACAGGGAATCTAATTGCTCCTAGG

GGTTACTTCAAAATACGAAGTGGGAAAAGCTCAATAATGAGATCAGAT

GCACCCATTGGCAAATGCAAGTCTGAATGCATCACTCCAAATGGAAG

CATTCCCAATGACAAACCATTCCAAAATGTAAACAGGATCACATACGG

GGCCTGTCCCAGATATGTTAAGCAAAGCACTCTGAAATTGGCAACAG

GAATGCGAAATGTACCAGAGAAACAAACTAGAGGCATATTTGGCGCA

ATAGCGGGTTTCATAGAAAATGGTTGGGAGGGAATGGTGGATGGTTG

GTACGGTTTCAGGCATCAAAATTCTGAGGGAAGAGGACAAGCAGCA

GATCTCAAAAGCACTCAAGCAGCAATCGATCAAATCAATGGGAAGCT

GAATCGATTGATCGGGAAAACCAACGAGAAATTCCATCAGATTGAGA

AAGAATTCTCAGAAGTAGAAGGGAGAATTCAGGACCTTGAGAAATAT

GTTGAGGACACAAAAATAGATCTCTGGTCATACAACGCAGAGCTTCT

TGTTGCCCTGGAAAACCAACATACAATTGATCTAACTGACTCAGAAAT

GAACAAACTGTTTGAAAAAACAAAGAAGCAACTGAGGGAAAATGCTG

AGGATATGGGCAATGGTTGTTTCAAAATATACCACAAATGTGACAATG

CCTGCATAGGATCAATCAGAAATGGAACTTATGACCACGATGTATACA

GGGATGAAGCATTAAACAACCGGTTCCAGATCAAGGGAGTTGAGCTG

AAGTCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCA

TGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCATCATGTGGGCCTGC

CAAAAGGGCAACATTAGGTGCAACATTTGCATTTGAGTGCATTAATTA

AAAACACCCTTGTTTCTACTGGAAGCGGAGCCACGAACTTCTCTCTG

TTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTATTTTCTA

ATATCCACAAAATGAAGGCAATAATTGTACTACTCATGGTAGTAACAT

CCAATGCAGATCGAATCTGCACTGGGATAACATCGTCAAACTCACCA

CATGTCGTCAAAACTGCTACTCAAGGGGAGGTCAACGTGACCGGTGT

AATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTCAA

AGGAACAGAAACCAGGGGGAAACTATGCCCAAAATGCCTCAACTGCA

CAGATCTGGATGTAGCCTTGGGCAGACCAAAATGCACAGGGAAAATA

CCCTCTGCAAGGGTTTCAATACTCCATGAAGTCAGACCTGTTACATCT

GGGTGCTTTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCC

TAACCTTCTCCGAGGATACGAACATGTCAGGTTATCAACTCACAACGT

TATCAATGCAAAAGATGCACCAGGAAGACCCTACGAAATTGGAACCT

CAGGGTCTTGCCCTAACATTACCAATGGAAACGGATTCTTCGCAACA

ATGGCTTGGGCCGTCCCAAAAAACAAAACAGCAACAAATCCATTAAC

AATAGAAGTACCATACATTTGTACAGAAGGAGAAGACCAAATTACCGT

TTGGGGGTTCCACTCTGACAACGAGACCCAAATGGCAAAGCTCTATG

GGGACTCAAAGCCCCAGAAGTTCACCTCATCTGCCAACGGAGTGAC

CACACATTACGTTTCACAGATTGGTGGCTTCCCAAATCAAACAGAAGA

CGGAGGACTACCACAAAGTGGCAGAATTGTTGTTGATTACATGGTGC

AGAAATCTGGAAAAACAGGAACAATTACCTATCAAAGAGGTATTTTAT

TGCCTCAAAAAGTGTGGTGCGCAAGTGGCAGGAGCAAGGTAATAAAA

GGATCCTTGCCCTTAATTGGAGAAGCAGATTGCCTCCATGAAAAATA

CGGTGGATTAAACAAAAGCAAGCCTTACTACACAGGGGAACATGCAA

AGGCCATAGGAAATTGCCCAATATGGGTGAAAACACCCTTGAAGCTG

GCCAATGGAACCAAATATAGACCCCCTGCAAAACTATTAAAGGAAAG

AGGTTTCTTCGGAGCCATTGCTGGTTTCTTAGAGGGAGGATGGGAAG

GAATGATTGCAGGTTGGCACGGATACACATCCCATGGGGCACATGG

AGTAGCGGTGGCAGCTGACCTTAAGAGCACTCAAGAGGCCATAAAC

AAGATAACAAAAAATCTCAACTCTTTGAGTGAGCTGGAAGTAAAGAAT

CTTCAAAGACTAAGCGGTGCCATGGATGAACTCCACAACGAAATACT

AGAACTAGATGAGAAAGTGGATGATCTCAGAGCTGACACAATAAGCT

CACAAATAGAACTCGCAGTCCTGCTTTCCAATGAAGGAATAATAAACA

GTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAAGAAAATG

CTGGGCCCCTCTGCTGTAGAGATAGGGAATGGATGCTTTGAAACCAA

ACACAAGTGCAACCAGACCTGCCTCGACAGAATAGCTGCTGGTACCT

TTGATGCAGGAGAATTTTCTCTCCCCACCTTTGATTCACTGAATATTA

CTGCTGCATCTTTAAATGACGACGGATTGGACAATCATACTATACTGC

TTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACACTGATGATAG

CTATCTTTGTTGTTTATATGGTCTCCAGAGACAATGTTTCTTGCTCCAT

TTGTCTATAAGGAAAGTTAAGCCCTGTATTTTCCTTTATTGTAGTGCTT

GTTTGCTTGTTGTCATTACAAAGAAACGTTATTGAAAAATGGAAGCGG

AGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAAA

ACCCCGGGCCTTTCGGAGCTATTGCTGGTTTCTTGGAAGGAGGATG

GGAAGGAATGATTGCAGGTTGGCACGGATACACATCTCATGGAGCA

CATGGAGTAGCAGTGGCAGCAGACCTTAAGAGTACCCATGATGACTC

GAGCTGGTACTGCATGCACGCAATGCTAGCTGCCCCTTTCCCGTCCT

GGGTACCCCGAGTCTCCCCCGACCTCGGGTCCCAGGTATGCTCCCA

CCTCCACCTGCCCCACTCACCACCTCTGCTAGTTCCAGACACCTCCC

AAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCC

CACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC

TAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACACC

CTGGAGCTAGCAAAAAAAA

Pan-CoV-Flu
CTCGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGT
397

Vaccine #9
CATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGG

CoV- Omicron
TAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGAC

BA.2
GTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCA

Spike(HexaPro)-
TTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAG

Influenza (HA)
TACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATG

ACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGG

GACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACC

ATGGTCGAGGTGAGCCCCACGTTCTGCTTCACTCTCCCCATCTCCCC

CCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTTTGT

GCAGCGATGGGGGCGGGGGGGGGGGGGGGGCGCGCGCCAGGCG

GGGCGGGGCGGGGCGAGGGGCGGGGCGGGGCGAGGCGGAGAGG

TGCGGCGGCAGCCAATCAGAGCGGCGCGCTCCGAAAGTTTCCTTTT

ATGGCGAGGCGGCGGCGGCGGCGGCCCTATAAAAAGCGAAGCGCG

CGGCGGGCGGGAGTCGCTGCGCGCTGCCTTCGCCCCGTGCCCCGC

TCCGCCGCCGCCTCGCGCCGCCCGCCCCGGCTCTGACTGACCGCG

TTACTCCCACAGGTGAGCGGGCGGGACGGCCCTTCTCCTCCGGGCT

GTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGC

GTGAAAGCCTTGAGGGGCTCCGGGAGGGCCCTTTGTGCGGGGGGA

GCGGCTCGGGGGGTGCGTGCGTGTGTGTGTGCGTGGGGAGCGCCG

CGTGCGGCTCCGCGCTGCCCGGCGGCTGTGAGCGCTGCGGGCGCG

GCGCGGGGCTTTGTGCGCTCCGCAGTGTGCGCGAGGGGAGCGCGG

CCGGGGGCGGTGCCCCGCGGTGCGGGGGGGGCTGCGAGGGGAAC

AAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGGGTGAGCAGGGGGT

GTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTGCACCCCCCTCCC

CGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGGGCTCCGTACG

GGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGGTGGCGGCA

GGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCCGGGGAG

GGCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGCGGCTG

TCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCGTGC

GAGAGGGCGCAGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCGA

AATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGA

AGCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTC

GTGCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGG

CTGTCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGG

CGGGGTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGC

TAACCATGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGT

GCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAGAATTGGAGAATAA

ACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGAACCCGCCACCA

TGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGT

GAACCTGATCACCAGGACCCAGAGCTACACCAACAGCTTCACCAGG

GGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCTGCACA

GCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTGGTTC

CACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGACA

ACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGAC

AGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGG

TGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGAC

GTGTACTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCA

GGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCA

GCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAAC

CTGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTA

CAGCAAGCACACCCCCATCAACCTGGGCAGGGACCTGCCCCAGGGC

TTCAGCGCCCTGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACA

TCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGAC

CCCCGGCGACAGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTA

CTACGTGGGCTACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAAC

GAGAACGGCACCATCACCGACGCCGTGGACTGCGCCCTGGACCCC

CTGAGCGAGACCAAGTGCACCCTGAAGAGCTTCACCGTGGAGAAGG

GCATCTACCAGACCAGCAACTTCAGGGTGCAGCCCACCGAGAGCAT

CGTGAGGTTCCCCAACATCACCAACCTGTGCCCCTTCGACGAGGTGT

TCAACGCCACCAGGTTCGCCAGCGTGTACGCCTGGAACAGGAAGAG

GATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTACAACTTCGCC

CCCTTCTTCGCCTTCAAGTGCTACGGCGTGAGCCCCACCAAGCTGAA

CGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTCGTGATCAGG

GGCAACGAGGTGAGCCAGATCGCCCCCGGCCAGACCGGCAACATC

GCCGACTACAACTACAAGCTGCCCGACGACTTCACCGGCTGCGTGA

TCGCCTGGAACAGCAACAAGCTGGACAGCAAGGTGGGCGGCAACTA

CAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGAAGCCCTTCG

AGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAACAAGCCCTG

CAACGGCGTGGCCGGCTTCAACTGCTACTTCCCCCTGAGGAGCTAC

GGCTTCAGGCCCACCTACGGCGTGGGCCACCAGCCCTACAGGGTG

GTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGTGCG

GCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACTT

CAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAAC

AAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACA

CCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACAT

CACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACC

AACACCAGCAACCAGGTGGCCGTGCTGTACCAGGGCGTGAACTGCA

CCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTG

GAGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGG

CTGCCTGATCGGCGCCGAGTACGTGAACAACAGCTACGAGTGCGAC

ATCCCCATCGGCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCA

AGAGCCACCAGCAGGCCCAGAGCGTGGCCAGCCAGAGCATCATCG

CCTACACCATGAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAA

CAACAGCATCGCCATCCCCACCAACTTCACCATCAGCGTGACCACCG

AGATCCTGCCCGTGAGCATGACCAAGACCAGCGTGGACTGCACCAT

GTACATCTGCGGCGACAGCACCGAGTGCAGCAACCTGCTGCTGCAG

TACGGCAGCTTCTGCACCCAGCTGAAGAGGGCCCTGACCGGCATCG

CCGTGGAGCAGGACAAGAACACCCAGGAGGTGTTCGCCCAGGTGAA

GCAGATCTACAAGACCCCCCCCATCAAGTACTTCGGCGGCTTCAACT

TCAGCCAGATCCTGCCCGACCCCAGCAAGCCCAGCAAGAGGAGCCC

CATCGAGGACCTGCTGTTCAACAAGGTGACCCTGGCCGACGCCGGC

TTCATCAAGCAGTACGGCGACTGCCTGGGCGACATCGCCGCCAGGG

ACCTGATCTGCGCCCAGAAGTTCAACGGCCTGACCGTGCTGCCCCC

CCTGCTGACCGACGAGATGATCGCCCAGTACACCAGCGCCCTGCTG

GCCGGCACCATCACCAGCGGCTGGACCTTCGGCGCCGGCCCCGCC

CTGCAGATCCCCTTCCCCATGCAGATGGCCTACAGGTTCAACGGCAT

CGGCGTGACCCAGAACGTGCTGTACGAGAACCAGAAGCTGATCGCC

AACCAGTTCAACAGCGCCATCGGCAAGATCCAGGACAGCCTGAGCA

GCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGTGGTGAACCACAA

CGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAGCAGCAAGTTC

GGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAGGCTGGACC

CCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCGGCAGGC

TGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGGGCCGC

CGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAGCGA

GTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGGG

CTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTG

TTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCA

CCGCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGA

GGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGG

AACTTCTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAG

CGGCAACTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTAC

GACCCCCTGCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACA

AGTACTTCAAGAACCACACCAGCCCCGACGTGGACCTGGGCGACAT

CAGCGGCATCAACGCCAGCGTGGTGAACATCCAGAAGGAGATCGAC

AGGCTGAACGAGGTGGCCAAGAACCTGAACGAGAGCCTGATCGACC

TGCAGGAGCTGGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGTA

CATCTGGCTGGGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTG

ACCATCATGCTGTGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGG

GCTGCTGCAGCTGCGGCAGCTGCTGCAAGTTCGACGAGGACGACAG

CGAGCCCGTGCTGAAGGGCGTGAAGCTGCACTACACCGGAAGCGG

AGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAAA

ACCCCGGGCCTATGAAGGCAATACTAGTAGTTCTGCTATATACATTTG

CAACCGCAAATGCAGACACATTATGTATAGGTTATCATGCGAACAACT

CAACAGACACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACA

CACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAA

CTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGCTGG

CTGGATCCTGGGAAATCCAGAGTGTGAATCACTCTCCACAGCAAGCT

CATGGTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTT

ACCCAGGAGATTTCATCGATTATGAGGAGCTAAGAGAGCAATTGAGC

TCAGTGTCATCATTTGAAAGGTTTGAGATATTCCCCAAGACAAGTTCA

TGGCCCAATCATGACTCGAACAAAGGTGTAACGACAGCATGTCCTCA

TGCTGGAGCAAAAAGCTTCTACAAAAATTTAATATGGCTAGTTAAAAA

AGGAAATTCATACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGG

GAAAGAAGTCCTCGTGCTATGGGGCATTCACCATCCACCTACTAGTG

CTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTTTTGTGG

GGACATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGG

CCCAAAGTGAGGGATCAAGAAGGGAGAATGAACTATTACTGGACACT

AGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAG

TGGTACCGAGATATGCATTCGCAATGGAAAGAAATGCTGGATCTGGT

ATTATCATTTCAGATACACCAGTCCACGATTGCAATACAACTTGTCAG

ACACCCAAGGGTGCTATAAACACCAGCCTCCCATTTCAGAACATACA

TCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGCACAAAATT

GAGACTGGCCACAGGATTGAGGAATGTCCCGTCCATTCAATCTAGAG

GCCTATTTGGGGCCATTGCCGGTTTCATTGAAGGGGGGTGGACAGG

GATGGTAGATGGATGGTACGGTTATCACCATCAAAATGAGCAGGGGT

CAGGATATGCAGCCGACCTGAAGAGCACACAGAATGCCATTGACGA

GATTACTAACAAAGTAAACTCTGTTATTGAAAAAATGAATACACAGTTC

ACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGAA

TTTAAATAAAAAAGTTGATGATGGTTTCCTGGACATTTGGACTTACAAT

GCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCA

CGATTCAAAGGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGTTAAA

AAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACCACAA

ATGTGATAACACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACT

ACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGAT

GGGGTAAAGCTGGAATCAACAAGGATTTACCAGATTTTGGCGATCTA

TTCAACCGTCGCCAGTTCATTGGTACTGGTAGTCTCCCTGGGGGCAA

TCAGTTTCTGGATGTGCTCTAATGGGTCTCTACAGTGTAGAATATGTA

TTTAAGGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGA

GATGTTGAAGAAAACCCCGGGCCTAGCAAAAGCAGGGGATAATTCTA

TTAACCATGAAGACTATCATTGCTTTGAGCTACATTCTATGTCTGGTTT

TCGCTCAAAAACTTCCTGGAAATGACAATAGCACTGCAACGCTGTGC

CTTGGGCACCATGCAGTACCAAACGGAACGATAGTGAAAACAATCAC

GAATGACCAAATTGAAGTTACTAATGCTACTGAGCTGGTTCAGAATTC

CTCAATAGGTGAAATATGCGACAGTCCTCATCAGATCCTTGATGGAG

AAAACTGCACACTAATAGATGCTCTATTGGGAGACCCTCAGTGTGAT

GGCTTTCAAAATAAGAAATGGGACCTTTTTGTTGAACGAAGCAAAGC

CTACAGCAACTGTTACCCTTATGATGTGCCGGATTATGCCTCCCTTAG

GTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGAAAGCT

TCAATTGGGCTGGAGTCACTCAAAACGGAACAAGTTCTGCTTGCATA

AGGGGATCTAATAGTAGTTTCTTTAGTAGATTAAATTGGTTGACCCAC

TTAAACTTCAAGTACCCAGCATTGAACGTGACTATGCCAAACAATGAA

CAATTTGACAAATTGTACATTTGGGGGGTTCACCACCCGGGTACGGA

CAAGGACCAAATCTTCCTGTATGCTCAATCATCAGGAAGAATCACAGT

ATCTACCAAAAGAAGCCAACAAGCTGTAATCCCGAATATCGGATCTA

GACCCAGAATAAGGAATATCCCTAGCAGAATAAGCATCTATTGGACA

ATAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAGGGAATCTA

ATTGCTCCTAGGGGTTACTTCAAAATACGAAGTGGGAAAAGCTCAAT

AATGAGATCAGATGCACCCATTGGCAAATGCAAGTCTGAATGCATCA

CTCCAAATGGAAGCATTCCCAATGACAAACCATTCCAAAATGTAAACA

GGATCACATACGGGGCCTGTCCCAGATATGTTAAGCAAAGCACTCTG

AAATTGGCAACAGGAATGCGAAATGTACCAGAGAAACAAACTAGAGG

CATATTTGGCGCAATAGCGGGTTTCATAGAAAATGGTTGGGAGGGAA

TGGTGGATGGTTGGTACGGTTTCAGGCATCAAAATTCTGAGGGAAGA

GGACAAGCAGCAGATCTCAAAAGCACTCAAGCAGCAATCGATCAAAT

CAATGGGAAGCTGAATCGATTGATCGGGAAAACCAACGAGAAATTCC

ATCAGATTGAGAAAGAATTCTCAGAAGTAGAAGGGAGAATTCAGGAC

CTTGAGAAATATGTTGAGGACACAAAAATAGATCTCTGGTCATACAAC

GCAGAGCTTCTTGTTGCCCTGGAAAACCAACATACAATTGATCTAACT

GACTGAGAAATGAACAAACTGTTTGAAAAAACAAAGAAGCAACTGAG

GGAAAATGCTGAGGATATGGGCAATGGTTGTTTCAAAATATACCACA

AATGTGACAATGCCTGCATAGGATCAATCAGAAATGGAACTTATGAC

CACGATGTATACAGGGATGAAGCATTAAACAACCGGTTCCAGATCAA

GGGAGTTGAGCTGAAGTCAGGGTACAAAGATTGGATCCTATGGATTT

CCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCAT

CATGTGGGCCTGCCAAAAGGGCAACATTAGGTGCAACATTTGCATTT

GAGTGCATTAATTAAAAACACCCTTGTTTCTACTGGAAGCGGAGCCA

CGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCC

GGGCCTATTTTCTAATATCCACAAAATGAAGGCAATAATTGTACTACT

CATGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACAT

CGTCAAACTCACCACATGTCGTCAAAACTGCTACTCAAGGGGAGGTC

AACGTGACCGGTGTAATACCACTGACAACAACACCCACCAAATCTCA

TTTTGCAAATCTCAAAGGAACAGAAACCAGGGGGAAACTATGCCCAA

AATGCCTCAACTGCACAGATCTGGATGTAGCCTTGGGCAGACCAAAA

TGCACAGGGAAAATACCCTCTGCAAGGGTTTCAATACTCCATGAAGT

CAGACCTGTTACATCTGGGTGCTTTCCTATAATGCACGATAGAACAAA

AATTAGACAGCTGCCTAACCTTCTCCGAGGATACGAACATGTCAGGT

TATCAACTCACAACGTTATCAATGCAAAAGATGCACCAGGAAGACCC

TACGAAATTGGAACCTCAGGGTCTTGCCCTAACATTACCAATGGAAA

CGGATTCTTCGCAACAATGGCTTGGGCCGTCCCAAAAAACAAAACAG

CAACAAATCCATTAACAATAGAAGTACCATACATTTGTACAGAAGGAG

AAGACCAAATTACCGTTTGGGGGTTCCACTCTGACAACGAGACCCAA

ATGGCAAAGCTCTATGGGGACTCAAAGCCCCAGAAGTTCACCTCATC

TGCCAACGGAGTGACCACACATTACGTTTCACAGATTGGTGGCTTCC

CAAATCAAACAGAAGACGGAGGACTACCACAAAGTGGCAGAATTGTT

GTTGATTACATGGTGCAGAAATCTGGAAAAACAGGAACAATTACCTAT

CAAAGAGGTATTTTATTGCCTCAAAAAGTGTGGTGCGCAAGTGGCAG

GAGCAAGGTAATAAAAGGATCCTTGCCCTTAATTGGAGAAGCAGATT

GCCTCCATGAAAAATACGGTGGATTAAACAAAAGCAAGCCTTACTAC

ACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGGTGAA

AACACCCTTGAAGCTGGCCAATGGAACCAAATATAGACCCCCTGCAA

AACTATTAAAGGAAAGAGGTTTCTTCGGAGCCATTGCTGGTTTCTTAG

AGGGAGGATGGGAAGGAATGATTGCAGGTTGGCACGGATACACATC

CCATGGGGCACATGGAGTAGCGGTGGCAGCTGACCTTAAGAGCACT

CAAGAGGCCATAAACAAGATAACAAAAAATCTCAACTCTTTGAGTGAG

CTGGAAGTAAAGAATCTTCAAAGACTAAGCGGTGCCATGGATGAACT

CCACAACGAAATACTAGAACTAGATGAGAAAGTGGATGATCTCAGAG

CTGACACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTTCCAATG

AAGGAATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGA

AAGCTGAAGAAAATGCTGGGCCCCTCTGCTGTAGAGATAGGGAATG

GATGCTTTGAAACCAAACACAAGTGCAACCAGACCTGCCTCGACAGA

ATAGCTGCTGGTACCTTTGATGCAGGAGAATTTTCTCTCCCCACCTTT

GATTCACTGAATATTACTGCTGCATCTTTAAATGACGACGGATTGGAC

AATCATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCT

GTAACACTGATGATAGCTATCTTTGTTGTTTATATGGTCTCCAGAGAC

AATGTTTCTTGCTCCATTTGTCTATAAGGAAAGTTAAGCCCTGTATTTT

CCTTTATTGTAGTGCTTGTTTGCTTGTTGTCATTACAAAGAAACGTTAT

TGAAAAATGGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCA

GGAGATGTTGAAGAAAACCCCGGGCCTTTCGGAGCTATTGCTGGTTT

CTTGGAAGGAGGATGGGAAGGAATGATTGCAGGTTGGCACGGATAC

ACATCTCATGGAGCACATGGAGTAGCAGTGGCAGCAGACCTTAAGA

GTACCCATGATGACTCGAGCTGGTACTGCATGCACGCAATGCTAGCT

GCCCCTTTCCCGTCCTGGGTACCCCGAGTCTCCCCCGACCTCGGGT

CCCAGGTATGCTCCCACCTCCACCTGCCCCACTCACCACCTCTGCTA

GTTCCAGACACCTCCCAAGCACGCAGCAATGCAGCTCAAAACGCTTA

GCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAA

TAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTT

CGTGCCAGCCACACCCTGGAGCTAGCAAAAAAAA

Pan-CoV-Flu
CTCGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGT
398

Vaccine #10
CATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGG

CoV-Omicron
TAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGAC

BA.2 Spike(2
GTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCA

proline
TTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAG

substitution)-
TACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATG

Influenza (HA)
ACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGG

GACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACC

ATGGTCGAGGTGAGCCCCACGTTCTGCTTCACTCTCCCCATCTCCCC

CCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTTTGT

GCAGCGATGGGGGCGGGGGGGGGGGGGGGGCGCGCGCCAGGCG

GGGCGGGGCGGGGCGAGGGGCGGGGCGGGGCGAGGCGGAGAGG

TGCGGCGGCAGCCAATCAGAGCGGCGCGCTCCGAAAGTTTCCTTTT

ATGGCGAGGCGGCGGCGGCGGCGGCCCTATAAAAAGCGAAGCGCG

CGGCGGGCGGGAGTCGCTGCGCGCTGCCTTCGCCCCGTGCCCCGC

TCCGCCGCCGCCTCGCGCCGCCCGCCCCGGCTCTGACTGACCGCG

TTACTCCCACAGGTGAGCGGGCGGGACGGCCCTTCTCCTCCGGGCT

GTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGC

GTGAAAGCCTTGAGGGGCTCCGGGAGGGCCCTTTGTGCGGGGGGA

GCGGCTCGGGGGGTGCGTGCGTGTGTGTGTGCGTGGGGAGCGCCG

CGTGCGGCTCCGCGCTGCCCGGCGGCTGTGAGCGCTGCGGGCGCG

GCGCGGGGCTTTGTGCGCTCCGCAGTGTGCGCGAGGGGAGCGCGG

CCGGGGGCGGTGCCCCGCGGTGCGGGGGGGGCTGCGAGGGGAAC

AAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGGGTGAGCAGGGGGT

GTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTGCACCCCCCTCCC

CGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGGGCTCCGTACG

GGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGGTGGCGGCA

GGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCCGGGGAG

GGCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGCGGCTG

TCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCGTGC

GAGAGGGCGCAGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCGA

AATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGA

AGCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTC

GTGCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGG

CTGTCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGG

CGGGGTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGC

TAACCATGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGT

GCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAGAATTGGAGAATAA

ACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGAACCCGCCACCA

TGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGT

GAACCTGATCACCAGGACCCAGAGCTACACCAACAGCTTCACCAGG

GGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCTGCACA

GCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTGGTTC

CACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGACA

ACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGAC

AGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGG

TGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGAC

GTGTACTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCA

GGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCA

GCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAAC

CTGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTA

CAGCAAGCACACCCCCATCAACCTGGGCAGGGACCTGCCCCAGGGC

TTCAGCGCCCTGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACA

TCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGAC

CCCCGGCGACAGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTA

CTACGTGGGCTACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAAC

GAGAACGGCACCATCACCGACGCCGTGGACTGCGCCCTGGACCCC

CTGAGCGAGACCAAGTGCACCCTGAAGAGCTTCACCGTGGAGAAGG

GCATCTACCAGACCAGCAACTTCAGGGTGCAGCCCACCGAGAGCAT

CGTGAGGTTCCCCAACATCACCAACCTGTGCCCCTTCGACGAGGTGT

TCAACGCCACCAGGTTCGCCAGCGTGTACGCCTGGAACAGGAAGAG

GATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTACAACTTCGCC

CCCTTCTTCGCCTTCAAGTGCTACGGCGTGAGCCCCACCAAGCTGAA

CGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTCGTGATCAGG

GGCAACGAGGTGAGCCAGATCGCCCCCGGCCAGACCGGCAACATC

GCCGACTACAACTACAAGCTGCCCGACGACTTCACCGGCTGCGTGA

TCGCCTGGAACAGCAACAAGCTGGACAGCAAGGTGGGCGGCAACTA

CAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGAAGCCCTTCG

AGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAACAAGCCCTG

CAACGGCGTGGCCGGCTTCAACTGCTACTTCCCCCTGAGGAGCTAC

GGCTTCAGGCCCACCTACGGCGTGGGCCACCAGCCCTACAGGGTG

GTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGTGCG

GCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACTT

CAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAAC

AAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACA

CCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACAT

CACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACC

AACACCAGCAACCAGGTGGCCGTGCTGTACCAGGGCGTGAACTGCA

CCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTG

GAGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGG

CTGCCTGATCGGCGCCGAGTACGTGAACAACAGCTACGAGTGCGAC

ATCCCCATCGGCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCA

AGAGCCACCAGCAGGCCCAGAGCGTGGCCAGCCAGAGCATCATCG

CCTACACCATGAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAA

CAACAGCATCGCCATCCCCACCAACTTCACCATCAGCGTGACCACCG

AGATCCTGCCCGTGAGCATGACCAAGACCAGCGTGGACTGCACCAT

GTACATCTGCGGCGACAGCACCGAGTGCAGCAACCTGCTGCTGCAG

TACGGCAGCTTCTGCACCCAGCTGAAGAGGGCCCTGACCGGCATCG

CCGTGGAGCAGGACAAGAACACCCAGGAGGTGTTCGCCCAGGTGAA

GCAGATCTACAAGACCCCCCCCATCAAGTACTTCGGCGGCTTCAACT

TCAGCCAGATCCTGCCCGACCCCAGCAAGCCCAGCAAGAGGAGCTT

CATCGAGGACCTGCTGTTCAACAAGGTGACCCTGGCCGACGCCGGC

TTCATCAAGCAGTACGGCGACTGCCTGGGCGACATCGCCGCCAGGG

ACCTGATCTGCGCCCAGAAGTTCAACGGCCTGACCGTGCTGCCCCC

CCTGCTGACCGACGAGATGATCGCCCAGTACACCAGCGCCCTGCTG

GCCGGCACCATCACCAGCGGCTGGACCTTCGGCGCCGGCGCCGCC

CTGCAGATCCCCTTCGCCATGCAGATGGCCTACAGGTTCAACGGCAT

CGGCGTGACCCAGAACGTGCTGTACGAGAACCAGAAGCTGATCGCC

AACCAGTTCAACAGCGCCATCGGCAAGATCCAGGACAGCCTGAGCA

GCACCGCCAGCGCCCTGGGCAAGCTGCAGGACGTGGTGAACCACA

ACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAGCAGCAAGTT

CGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAGGCTGGAC

CCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCGGCAGG

CTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGGGCCG

CCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAGCG

AGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGG

GCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGT

GTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACC

ACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGG

AGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAG

GAACTTCTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGA

GCGGCAACTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTA

CGACCCCCTGCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGAC

AAGTACTTCAAGAACCACACCAGCCCCGACGTGGACCTGGGCGACA

TCAGCGGCATCAACGCCAGCGTGGTGAACATCCAGAAGGAGATCGA

CAGGCTGAACGAGGTGGCCAAGAACCTGAACGAGAGCCTGATCGAC

CTGCAGGAGCTGGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGT

ACATCTGGCTGGGCTTCATCGCCGGCCTGATCGCCATCGTGATGGT

GACCATCATGCTGTGCTGCATGACCAGCTGCTGCAGCTGCCTGAAG

GGCTGCTGCAGCTGCGGCAGCTGCTGCAAGTTCGACGAGGACGACA

GCGAGCCCGTGCTGAAGGGCGTGAAGCTGCACTACACCGGAAGCG

GAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAA

AACCCCGGGCCTATGAAGGCAATACTAGTAGTTCTGCTATATACATTT

GCAACCGCAAATGCAGACACATTATGTATAGGTTATCATGCGAACAA

CTCAACAGACACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAA

CACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGC

AAACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGC

TGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCTCCACAGCAA

GCTCATGGTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACG

TGTTACCCAGGAGATTTCATCGATTATGAGGAGCTAAGAGAGCAATT

GAGCTCAGTGTCATCATTTGAAAGGTTTGAGATATTCCCCAAGACAA

GTTCATGGCCCAATCATGACTCGAACAAAGGTGTAACGACAGCATGT

CCTCATGCTGGAGCAAAAAGCTTCTACAAAAATTTAATATGGCTAGTT

AAAAAAGGAAATTCATACCCAAAGCTCAGCAAATCCTACATTAATGAT

AAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTCACCATCCACCTAC

TAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTTT

TGTGGGGACATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAA

TAAGGCCCAAAGTGAGGGATCAAGAAGGGAGAATGAACTATTACTGG

ACACTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAA

TCTAGTGGTACCGAGATATGCATTCGCAATGGAAAGAAATGCTGGAT

CTGGTATTATCATTTCAGATACACCAGTCCACGATTGCAATACAACTT

GTCAGACACCCAAGGGTGCTATAAACACCAGCCTCCCATTTCAGAAC

ATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGCACA

AAATTGAGACTGGCCACAGGATTGAGGAATGTCCCGTCCATTCAATC

TAGAGGCCTATTTGGGGCCATTGCCGGTTTCATTGAAGGGGGGTGG

ACAGGGATGGTAGATGGATGGTACGGTTATCACCATCAAAATGAGCA

GGGGTCAGGATATGCAGCCGACCTGAAGAGCACACAGAATGCCATT

GACGAGATTACTAACAAAGTAAACTCTGTTATTGAAAAAATGAATACA

CAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAAT

AGAGAATTTAAATAAAAAAGTTGATGATGGTTTCCTGGACATTTGGAC

TTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGA

CTACCACGATTCAAAGGTGAAGAACTTATATGAAAAGGTAAGAAGCC

AGTTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTT

ACCACAAATGTGATAACACGTGCATGGAAAGTGTCAAAAATGGGACT

TATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAA

ATAGATGGGGTAAAGCTGGAATCAACAAGGATTTACCAGATTTTGGC

GATCTATTCAACCGTCGCCAGTTCATTGGTACTGGTAGTCTCCCTGG

GGGCAATCAGTTTCTGGATGTGCTCTAATGGGTCTCTACAGTGTAGA

ATATGTATTTAAGGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAA

GCAGGAGATGTTGAAGAAAACCCCGGGCCTAGCAAAAGCAGGGGAT

AATTCTATTAACCATGAAGACTATCATTGCTTTGAGCTACATTCTATGT

CTGGTTTTCGCTCAAAAACTTCCTGGAAATGACAATAGCACTGCAAC

GCTGTGCCTTGGGCACCATGCAGTACCAAACGGAACGATAGTGAAAA

CAATCACGAATGACCAAATTGAAGTTACTAATGCTACTGAGCTGGTTC

AGAATTCCTCAATAGGTGAAATATGCGACAGTCCTCATCAGATCCTTG

ATGGAGAAAACTGCACACTAATAGATGCTCTATTGGGAGACCCTCAG

TGTGATGGCTTTCAAAATAAGAAATGGGACCTTTTTGTTGAACGAAGC

AAAGCCTACAGCAACTGTTACCCTTATGATGTGCCGGATTATGCCTC

CCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG

AAAGCTTCAATTGGGCTGGAGTCACTCAAAACGGAACAAGTTCTGCT

TGCATAAGGGGATCTAATAGTAGTTTCTTTAGTAGATTAAATTGGTTG

ACCCACTTAAACTTCAAGTACCCAGCATTGAACGTGACTATGCCAAAC

AATGAACAATTTGACAAATTGTACATTTGGGGGGTTCACCACCCGGG

TACGGACAAGGACCAAATCTTCCTGTATGCTCAATCATCAGGAAGAA

TCACAGTATCTACGAAAAGAAGCCAACAAGCTGTAATGCGGAATATC

GGATCTAGACCCAGAATAAGGAATATCCCTAGCAGAATAAGCATCTA

TTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAG

GGAATCTAATTGCTCCTAGGGGTTACTTCAAAATACGAAGTGGGAAA

AGCTCAATAATGAGATCAGATGCACCCATTGGCAAATGCAAGTCTGA

ATGCATCACTCCAAATGGAAGCATTCCCAATGACAAACCATTCCAAAA

TGTAAACAGGATCACATACGGGGCCTGTCCCAGATATGTTAAGCAAA

GCACTCTGAAATTGGCAACAGGAATGCGAAATGTACCAGAGAAACAA

ACTAGAGGCATATTTGGCGCAATAGCGGGTTTCATAGAAAATGGTTG

GGAGGGAATGGTGGATGGTTGGTACGGTTTCAGGCATCAAAATTCTG

AGGGAAGAGGACAAGCAGCAGATCTCAAAAGCACTCAAGCAGCAAT

CGATCAAATCAATGGGAAGCTGAATCGATTGATCGGGAAAACCAACG

AGAAATTCCATCAGATTGAGAAAGAATTCTCAGAAGTAGAAGGGAGA

ATTCAGGACCTTGAGAAATATGTTGAGGACACAAAAATAGATCTCTGG

TCATACAACGCAGAGCTTCTTGTTGCCCTGGAAAACCAACATACAATT

GATCTAACTGACTCAGAAATGAACAAACTGTTTGAAAAAACAAAGAAG

CAACTGAGGGAAAATGCTGAGGATATGGGCAATGGTTGTTTCAAAAT

ATACCACAAATGTGACAATGCCTGCATAGGATCAATCAGAAATGGAA

CTTATGACCACGATGTATACAGGGATGAAGCATTAAACAACCGGTTC

CAGATCAAGGGAGTTGAGCTGAAGTCAGGGTACAAAGATTGGATCCT

ATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTG

GGGTTCATCATGTGGGCCTGCCAAAAGGGCAACATTAGGTGCAACAT

TTGCATTTGAGTGCATTAATTAAAAACACCCTTGTTTCTACTGGAAGC

GGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGA

AAACCCCGGGCCTATTTTCTAATATCCACAAAATGAAGGCAATAATTG

TACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGG

ATAACATCGTCAAACTCACCACATGTCGTCAAAACTGCTACTCAAGG

GGAGGTCAACGTGACCGGTGTAATACCACTGACAACAACACCCACCA

AATCTCATTTTGCAAATCTCAAAGGAACAGAAACCAGGGGGAAACTAT

GCCCAAAATGCCTCAACTGCACAGATCTGGATGTAGCCTTGGGCAGA

CCAAAATGCACAGGGAAAATACCCTCTGCAAGGGTTTCAATACTCCA

TGAAGTCAGACCTGTTACATCTGGGTGCTTTCCTATAATGCACGATAG

AACAAAAATTAGACAGCTGCCTAACCTTCTCCGAGGATACGAACATG

TCAGGTTATCAACTCACAACGTTATCAATGCAAAAGATGCACCAGGAA

GACCCTACGAAATTGGAACCTCAGGGTCTTGCCCTAACATTACCAAT

GGAAACGGATTCTTCGCAACAATGGCTTGGGCCGTCCCAAAAAACAA

AACAGCAACAAATCCATTAACAATAGAAGTACCATACATTTGTACAGA

AGGAGAAGACCAAATTACCGTTTGGGGGTTCCACTCTGACAACGAGA

CCCAAATGGCAAAGCTCTATGGGGACTCAAAGCCCCAGAAGTTCACC

TCATCTGCCAACGGAGTGACCACACATTACGTTTCACAGATTGGTGG

CTTCCCAAATCAAACAGAAGACGGAGGACTACCACAAAGTGGCAGAA

TTGTTGTTGATTACATGGTGCAGAAATCTGGAAAAACAGGAACAATTA

CCTATCAAAGAGGTATTTTATTGCCTCAAAAAGTGTGGTGCGCAAGT

GGCAGGAGCAAGGTAATAAAAGGATCCTTGCCCTTAATTGGAGAAGC

AGATTGCCTCCATGAAAAATACGGTGGATTAAACAAAAGCAAGCCTTA

CTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG

TGAAAACACCCTTGAAGCTGGCCAATGGAACCAAATATAGACCCCCT

GCAAAACTATTAAAGGAAAGAGGTTTCTTCGGAGCCATTGCTGGTTT

CTTAGAGGGAGGATGGGAAGGAATGATTGCAGGTTGGCACGGATAC

ACATCCCATGGGGCACATGGAGTAGCGGTGGCAGCTGACCTTAAGA

GCACTCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACTCTTTGA

GTGAGCTGGAAGTAAAGAATCTTCAAAGACTAAGCGGTGCCATGGAT

GAACTCCACAACGAAATACTAGAACTAGATGAGAAAGTGGATGATCT

CAGAGCTGACACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT

CCAATGAAGGAATAATAAACAGTGAAGATGAACATCTCTTGGCGCTT

GAAAGAAAGCTGAAGAAAATGCTGGGCCCCTCTGCTGTAGAGATAG

GGAATGGATGCTTTGAAACCAAACACAAGTGCAACCAGACCTGCCTC

GACAGAATAGCTGCTGGTACCTTTGATGCAGGAGAATTTTCTCTCCC

CACCTTTGATTCACTGAATATTACTGCTGCATCTTTAAATGACGACGG

ATTGGACAATCATACTATACTGCTTTACTACTCAACTGCTGCCTCCAG

TTTGGCTGTAACACTGATGATAGCTATCTTTGTTGTTTATATGGTCTC

CAGAGACAATGTTTCTTGCTCCATTTGTCTATAAGGAAAGTTAAGCCC

TGTATTTTCCTTTATTGTAGTGCTTGTTTGCTTGTTGTCATTACAAAGA

AACGTTATTGAAAAATGGAAGCGGAGCCACGAACTTCTCTCTGTTAAA

GCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTTTCGGAGCTATT

GCTGGTTTCTTGGAAGGAGGATGGGAAGGAATGATTGCAGGTTGGC

ACGGATACACATCTCATGGAGCACATGGAGTAGCAGTGGCAGCAGA

CCTTAAGAGTACCCATGATGACTCGAGCTGGTACTGCATGCACGCAA

TGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCCGAGTCTCCCCCGA

CCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGCCCCACTCACCA

CCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCAATGCAGCTCA

AAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAAC

CTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTT

GGTCAATTTCGTGCCAGCCACACCCTGGAGCTAGCAAAAAAAA

Pan-CoV-Flu
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATAT
399

Vaccine #11
ATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTG

CoV-Wuhan
ACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTC

Spike(HexaPro)-
CCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAG

Influenza (HA)
TATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATG

CCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTG

GCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTA

CATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCA

GTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAA

GTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAAT

CAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCA

AATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT

GGTTTAGTGAACCGTCAGATCGAGAATAAACTAGTATTCTTCTGGTCC

CCACAGACTCAGAGAGAACCCGCCACCATGTTCGTGTTCCTGGTGCT

GCTGCCCCTGGTGAGCAGCCAGTGCGTGAACCTGACCACCAGGACC

CAGCTGCCCCCCGCCTACACCAACAGCTTCACCAGGGGCGTGTACT

ACCCCGACAAGGTGTTCAGGAGCAGCGTGCTGCACAGCACCCAGGA

CCTGTTCCTGCCCTTCTTCAGCAACGTGACCTGGTTCCACGCCATCC

ACGTGAGCGGCACCAACGGCACCAAGAGGTTCGACAACCCCGTGCT

GCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGAGAAGAGCAAC

ATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAGCAAGACCC

AGAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGATCAAGGT

GTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTACTACC

ACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTACAG

CAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCTG

ATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGT

TCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCAC

ACCCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCC

TGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTT

CCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGAC

AGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGC

TACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCA

CCATCACCGACGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGAC

CAAGTGCACCCTGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAG

ACCAGCAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTTCC

CCAACATCACCAACCTGTGCCCCTTCGGCGAGGTGTTCAACGCCAC

CAGGTTCGCCAGCGTGTACGCCTGGAACAGGAAGAGGATCAGCAAC

TGCGTGGCCGACTACAGCGTGCTGTACAACAGCGCCAGCTTCAGCA

CCTTCAAGTGCTACGGCGTGAGCCCCACCAAGCTGAACGACCTGTG

CTTCACCAACGTGTACGCCGACAGCTTCGTGATCAGGGGCGACGAG

GTGAGGCAGATCGCCCCCGGCCAGACCGGCAAGATCGCCGACTACA

ACTACAAGCTGCCCGACGACTTCACCGGCTGCGTGATCGCCTGGAA

CAGCAACAACCTGGACAGCAAGGTGGGCGGCAACTACAACTACCTG

TACAGGCTGTTCAGGAAGAGCAACCTGAAGCCCTTCGAGAGGGACA

TCAGCACCGAGATCTACCAGGCCGGCAGCACCCCCTGCAACGGCGT

GGAGGGCTTCAACTGCTACTTCCCCCTGCAGAGCTACGGCTTCCAG

CCCACCAACGGCGTGGGCTACCAGCCCTACAGGGTGGTGGTGCTGA

GCTTCGAGCTGCTGCACGCCCCCGCCACCGTGTGCGGCCCCAAGAA

GAGCACCAACCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACG

GCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACAAGAAGTTCCT

GCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCACCGACGCC

GTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACCCCCTGCA

GCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACACCAGCAA

CCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGGTGCCC

GTGGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTGTACA

GCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGATCG

GCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCGG

CGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCGGC

AGCGCCAGCAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGA

GCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGC

CATCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCG

TGAGCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGG

CGACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTC

TGCACCCAGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAG

GACAAGAACACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACA

AGACCCCCCCCATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATC

CTGCCCGACCCCAGCAAGCCCAGCAAGAGGAGCCCCATCGAGGAC

CTGCTGTTCAACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGC

AGTACGGCGACTGCCTGGGCGACATCGCCGCCAGGGACCTGATCTG

CGCCCAGAAGTTCAACGGCCTGACCGTGCTGCCCCCCCTGCTGACC

GACGAGATGATCGCCCAGTACACCAGCGCCCTGCTGGCCGGCACCA

TCACCAGCGGCTGGACCTTCGGCGCCGGCCCCGCCCTGCAGATCC

CCTTCCCCATGCAGATGGCCTACAGGTTCAACGGCATCGGCGTGAC

CCAGAACGTGCTGTACGAGAACCAGAAGCTGATCGCCAACCAGTTCA

ACAGCGCCATCGGCAAGATCCAGGACAGCCTGAGCAGCACCCCCAG

CGCCCTGGGCAAGCTGCAGGACGTGGTGAACCAGAACGCCCAGGC

CCTGAACACCCTGGTGAAGCAGCTGAGCAGCAACTTCGGCGCCATC

AGCAGCGTGCTGAACGACATCCTGAGCAGGCTGGACCCCCCCGAGG

CCGAGGTGCAGATCGACAGGCTGATCACCGGCAGGCTGCAGAGCCT

GCAGACCTACGTGACCCAGCAGCTGATCAGGGCCGCCGAGATCAGG

GCCAGCGCCAACCTGGCCGCCACCAAGATGAGCGAGTGCGTGCTG

GGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGGGCTACCACCTGA

TGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTGTTCCTGCACGT

GACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACCGCCCCCGCC

ATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGGCGTGTTCG

TGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTTCTACGA

GCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAACTGC

GACGTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCTGC

AGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATC

AACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACG

AGGTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCT

GGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTG

GGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCT

GTGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGC

TGCGGCAGCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTG

CTGAAGGGCGTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACT

TCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCT

ATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAAT

GCAGACACATTATGTATAGGTTATCATGCGAACAACTCAACAGACACT

GTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC

CTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGAGGGGT

AGCCCCATTGCATTTGGGTAAATGTAACATTGCTGGCTGGATCCTGG

GAAATCCAGAGTGTGAATCACTCTCCACAGCAAGCTCATGGTCCTAC

ATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAGGAGA

TTTCATCGATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTCAT

CATTTGAAAGGTTTGAGATATTCCCCAAGACAAGTTCATGGCCCAATC

ATGACTCGAACAAAGGTGTAACGACAGCATGTCCTCATGCTGGAGCA

AAAAGCTTCTACAAAAATTTAATATGGCTAGTTAAAAAAGGAAATTCAT

ACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGGGAAAGAAGTC

CTCGTGCTATGGGGCATTCACCATCCACCTACTAGTGCTGACCAACA

AAGTCTCTATCAGAATGCAGATGCATATGTTTTTGTGGGGACATCAAG

ATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGGCCCAAAGTGA

GGGATCAAGAAGGGAGAATGAACTATTACTGGACACTAGTAGAGCCG

GGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGTGGTACCGAG

ATATGCATTCGCAATGGAAAGAAATGCTGGATCTGGTATTATCATTTC

AGATACACCAGTCCACGATTGCAATACAACTTGTCAGACACCCAAGG

GTGCTATAAACACCAGCCTCCCATTTCAGAACATACATCCGATCACAA

TTGGAAAATGTCCAAAATATGTAAAAAGCACAAAATTGAGACTGGCCA

CAGGATTGAGGAATGTCCCGTCCATTCAATCTAGAGGCCTATTTGGG

GCCATTGCCGGTTTCATTGAAGGGGGGTGGACAGGGATGGTAGATG

GATGGTACGGTTATCACCATCAAAATGAGCAGGGGTCAGGATATGCA

GCCGACCTGAAGAGCACACAGAATGCCATTGACGAGATTACTAACAA

AGTAAACTCTGTTATTGAAAAAATGAATACACAGTTCACAGCAGTAGG

TAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGAATTTAAATAAAAA

AGTTGATGATGGTTTCCTGGACATTTGGACTTACAATGCCGAACTGTT

GGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAAGGT

GAAGAACTTATATGAAAAGGTAAGAAGCCAGTTAAAAAACAATGCCAA

GGAAATTGGAAACGGCTGCTTTGAATTTTACCACAAATGTGATAACAC

GTGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACT

CAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGGTAAAGCTG

GAATCAACAAGGATTTACCAGATTTTGGCGATCTATTCAACCGTCGCC

AGTTCATTGGTACTGGTAGTCTCCCTGGGGGCAATCAGTTTCTGGAT

GTGCTCTAATGGGTCTCTACAGTGTAGAATATGTATTTAAGGAAGCG

GAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAA

AACCCCGGGCCTAGCAAAAGCAGGGGATAATTCTATTAACCATGAAG

ACTATCATTGCTTTGAGCTACATTCTATGTCTGGTTTTCGCTCAAAAA

CTTCCTGGAAATGACAATAGCACTGCAACGCTGTGCCTTGGGCACCA

TGCAGTACCAAACGGAACGATAGTGAAAACAATCACGAATGACCAAA

TTGAAGTTACTAATGCTACTGAGCTGGTTCAGAATTCCTCAATAGGTG

AAATATGCGACAGTCCTCATCAGATCCTTGATGGAGAAAACTGCACA

CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAAT

AAGAAATGGGACCTTTTTGTTGAACGAAGCAAAGCCTACAGCAACTG

TTACCCTTATGATGTGCCGGATTATGCCTCCCTTAGGTCACTAGTTGC

CTCATCCGGCACACTGGAGTTTAACAATGAAAGCTTCAATTGGGCTG

GAGTCACTCAAAACGGAACAAGTTCTGCTTGCATAAGGGGATCTAAT

AGTAGTTTCTTTAGTAGATTAAATTGGTTGACCCACTTAAACTTCAAGT

ACCCAGCATTGAACGTGACTATGCCAAACAATGAACAATTTGACAAAT

TGTACATTTGGGGGGTTCACCACCCGGGTACGGACAAGGACCAAAT

CTTCCTGTATGCTCAATCATCAGGAAGAATCACAGTATCTACCAAAAG

AAGCCAACAAGCTGTAATCCCGAATATCGGATCTAGACCCAGAATAA

GGAATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAACCG

GGAGACATACTTTTGATTAACAGCACAGGGAATCTAATTGCTCCTAG

GGGTTACTTCAAAATACGAAGTGGGAAAAGCTCAATAATGAGATCAG

ATGCACCCATTGGCAAATGCAAGTCTGAATGCATCACTCCAAATGGA

AGCATTCCCAATGACAAACCATTCCAAAATGTAAACAGGATCACATAC

GGGGCCTGTCCCAGATATGTTAAGCAAAGCACTCTGAAATTGGCAAC

AGGAATGCGAAATGTACCAGAGAAACAAACTAGAGGCATATTTGGCG

CAATAGCGGGTTTCATAGAAAATGGTTGGGAGGGAATGGTGGATGGT

TGGTACGGTTTCAGGCATCAAAATTCTGAGGGAAGAGGACAAGCAGC

AGATCTCAAAAGCACTCAAGCAGCAATCGATCAAATCAATGGGAAGC

TGAATCGATTGATCGGGAAAACCAACGAGAAATTCCATCAGATTGAG

AAAGAATTCTCAGAAGTAGAAGGGAGAATTCAGGACCTTGAGAAATA

TGTTGAGGACACAAAAATAGATCTCTGGTCATACAACGCAGAGCTTC

TTGTTGCCCTGGAAAACCAACATACAATTGATCTAACTGACTCAGAAA

TGAACAAACTGTTTGAAAAAACAAAGAAGCAACTGAGGGAAAATGCT

GAGGATATGGGCAATGGTTGTTTCAAAATATACCACAAATGTGACAAT

GCCTGCATAGGATCAATCAGAAATGGAACTTATGACCACGATGTATA

CAGGGATGAAGCATTAAACAACCGGTTCCAGATCAAGGGAGTTGAGC

TGAAGTCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT

CATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCATCATGTGGGCCT

GCCAAAAGGGCAACATTAGGTGCAACATTTGCATTTGAGTGCATTAAT

TAAAAACACCCTTGTTTCTACTGGAAGCGGAGCCACGAACTTCTCTCT

GTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTATTTTCT

AATATCCACAAAATGAAGGCAATAATTGTACTACTCATGGTAGTAACA

TCCAATGCAGATCGAATCTGCACTGGGATAACATCGTCAAACTCACC

ACATGTCGTCAAAACTGCTACTCAAGGGGAGGTCAACGTGACCGGT

GTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC

AAAGGAACAGAAACCAGGGGGAAACTATGCCCAAAATGCCTCAACTG

CACAGATCTGGATGTAGCCTTGGGCAGACCAAAATGCACAGGGAAAA

TACCCTCTGCAAGGGTTTCAATACTCCATGAAGTCAGACCTGTTACAT

CTGGGTGCTTTCCTATAATGCACGATAGAACAAAAATTAGACAGCTG

CCTAACCTTCTCCGAGGATACGAACATGTCAGGTTATCAACTCACAA

CGTTATCAATGCAAAAGATGCACCAGGAAGACCCTACGAAATTGGAA

CCTCAGGGTCTTGCCCTAACATTACCAATGGAAACGGATTCTTCGCA

ACAATGGCTTGGGCCGTCCCAAAAAACAAAACAGCAACAAATCCATT

AACAATAGAAGTACCATACATTTTACAGAAGGAGAAGACCAAATTAC

CGTTTGGGGGTTCCACTCTGACAACGAGACCCAAATGGCAAAGCTCT

ATGGGGACTCAAAGCCCCAGAAGTTCACCTCATCTGCCAACGGAGT

GACCACACATTACGTTTCACAGATTGGTGGCTTCCCAAATCAAACAG

AAGACGGAGGACTACCACAAAGTGGCAGAATTGTTGTTGATTACATG

GTGCAGAAATCTGGAAAAACAGGAACAATTACCTATCAAAGAGGTATT

TTATTGCCTCAAAAAGTGTGGTGCGCAAGTGGCAGGAGCAAGGTAAT

AAAAGGATCCTTGCCCTTAATTGGAGAAGCAGATTGCCTCCATGAAA

AATACGGTGGATTAAACAAAAGCAAGCCTTACTACACAGGGGAACAT

GCAAAGGCCATAGGAAATTGCCCAATATGGGTGAAAACACCCTTGAA

GCTGGCCAATGGAACCAAATATAGACCCCCTGCAAAACTATTAAAGG

AAAGAGGTTTCTTCGGAGCCATTGCTGGTTTCTTAGAGGGAGGATGG

GAAGGAATGATTGCAGGTTGGCACGGATACACATCCCATGGGGCAC

ATGGAGTAGCGGTGGCAGCTGACCTTAAGAGCACTCAAGAGGCCAT

AAACAAGATAACAAAAAATCTCAACTCTTTGAGTGAGCTGGAAGTAAA

GAATCTTCAAAGACTAAGCGGTGCCATGGATGAACTCCACAACGAAA

TACTAGAACTAGATGAGAAAGTGGATGATCTCAGAGCTGACACAATA

AGCTCACAAATAGAACTCGCAGTCCTGCTTTCCAATGAAGGAATAATA

AACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAAGAA

AATGCTGGGCCCCTCTGCTGTAGAGATAGGGAATGGATGCTTTGAAA

CCAAACACAAGTGCAACCAGACCTGCCTCGACAGAATAGCTGCTGGT

ACCTTTGATGCAGGAGAATTTTCTCTCCCCACCTTTGATTCACTGAAT

ATTACTGCTGCATCTTTAAATGACGACGGATTGGACAATCATACTATA

CTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACACTGATG

ATAGCTATCTTTGTTGTTTATATGGTCTCCAGAGACAATGTTTCTTGCT

CCATTTGTCTATAAGGAAAGTTAAGCCCTGTATTTTCCTTTATTGTAGT

GCTTGTTTGCTTGTTGTCATTACAAAGAAACGTTATTGAAAAATGGAA

GCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAA

GAAAACCCCGGGCCTTTCGGAGCTATTGCTGGTTTCTTGGAAGGAG

GATGGGAAGGAATGATTGCAGGTTGGCACGGATACACATCTCATGGA

GCACATGGAGTAGCAGTGGCAGCAGACCTTAAGAGTACCCATGATG

ACTCGAGCTGGTACTGCATGCACGCAATGCTAGCTGCCCCTTTCCCG

TCCTGGGTACCCCGAGTCTCCCCCGACCTCGGGTCCCAGGTATGCT

CCCACCTCCACCTGCCCCACTCACCACCTCTGCTAGTTCCAGACACC

TCCCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACAC

CCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT

TAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCA

CACCCTGGAGCTAGCAAAAAAAA

Pan-CoV-Flu
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATAT
400

Vaccine #12
ATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTG

CoV-Wuhan
ACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTC

Spike(2 proline
CCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAG

substitution)-
TATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATG

influenza (HA)
CCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTG

GCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTA

CATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCA

GTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAA

GTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAAT

CAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCA

AATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT

GGTTTAGTGAACCGTCAGATCGAGAATAAACTAGTATTCTTCTGGTCC

CCACAGACTCAGAGAGAACCCGCCACCATGTTCGTGTTCCTGGTGCT

GCTGCCCCTGGTGAGCAGCCAGTGCGTGAACCTGACCACCAGGACC

CAGCTGCCCCCCGCCTACACCAACAGCTTCACCAGGGGCGTGTACT

ACCCCGACAAGGTGTTCAGGAGCAGCGTGCTGCACAGCACCCAGGA

CCTGTTCCTGCCCTTCTTCAGCAACGTGACCTGGTTCCACGCCATCC

ACGTGAGCGGCACCAACGGCACCAAGAGGTTCGACAACCCCGTGCT

GCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGAGAAGAGCAAC

ATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAGCAAGACCC

AGAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGATCAAGGT

GTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTACTACC

ACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTACAG

CAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCTG

ATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGT

TCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCAC

ACCCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCC

TGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTT

CCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGAC

AGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGC

TACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCA

CCATCACCGACGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGAC

CAAGTGCACCCTGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAG

ACCAGCAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTTCC

CCAACATCACCAACCTGTGCCCCTTCGGCGAGGTGTTCAACGCCAC

CAGGTTCGCCAGCGTGTACGCCTGGAACAGGAAGAGGATCAGCAAC

TGCGTGGCCGACTACAGCGTGCTGTACAACAGCGCCAGCTTCAGCA

CCTTCAAGTGCTACGGCGTGAGCCCCACCAAGCTGAACGACCTGTG

CTTCACCAACGTGTACGCCGACAGCTTCGTGATCAGGGGCGACGAG

GTGAGGCAGATCGCCCCCGGCCAGACCGGCAAGATCGCCGACTACA

ACTACAAGCTGCCCGACGACTTCACCGGCTGCGTGATCGCCTGGAA

CAGCAACAACCTGGACAGCAAGGTGGGCGGCAACTACAACTACCTG

TACAGGCTGTTCAGGAAGAGCAACCTGAAGCCCTTCGAGAGGGACA

TCAGCACCGAGATCTACCAGGCCGGCAGCACCCCCTGCAACGGCGT

GGAGGGCTTCAACTGCTACTTCCCCCTGCAGAGCTACGGCTTCCAG

CCCACCAACGGCGTGGGCTACCAGCCCTACAGGGTGGTGGTGCTGA

GCTTCGAGCTGCTGCACGCCCCCGCCACCGTGTGCGGCCCCAAGAA

GAGCACCAACCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACG

GCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACAAGAAGTTCCT

GCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCACCGACGCC

GTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACCCCCTGCA

GCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACACCAGCAA

CCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGGTGCCC

GTGGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTGTACA

GCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGATCG

GCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCGG

CGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCAGG

AGGGCCAGGAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGA

GCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGC

CATCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCG

TGAGCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGG

CGACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTC

TGCACCCAGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAG

GACAAGAACACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACA

AGACCCCCCCCATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATC

CTGCCCGACCCCAGCAAGCCCAGCAAGAGGAGCTTCATCGAGGACC

TGCTGTTCAACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGCA

GTACGGCGACTGCCTGGGCGACATCGCCGCCAGGGACCTGATCTGC

GCCCAGAAGTTCAACGGCCTGACCGTGCTGCCCCCCCTGCTGACCG

ACGAGATGATCGCCCAGTACACCAGCGCCCTGCTGGCCGGCACCAT

CACCAGCGGCTGGACCTTCGGCGCCGGCGCCGCCCTGCAGATCCC

CTTCGCCATGCAGATGGCCTACAGGTTCAACGGCATCGGCGTGACC

CAGAACGTGCTGTACGAGAACCAGAAGCTGATCGCCAACCAGTTCAA

CAGCGCCATCGGCAAGATCCAGGACAGCCTGAGCAGCACCGCCAGC

GCCCTGGGCAAGCTGCAGGACGTGGTGAACCAGAACGCCCAGGCC

CTGAACACCCTGGTGAAGCAGCTGAGCAGCAACTTCGGCGCCATCA

GCAGCGTGCTGAACGACATCCTGAGCAGGCTGGACCCCCCCGAGG

CCGAGGTGCAGATCGACAGGCTGATCACCGGCAGGCTGCAGAGCCT

GCAGACCTACGTGACCCAGCAGCTGATCAGGGCCGCCGAGATCAGG

GCCAGCGCCAACCTGGCCGCCACCAAGATGAGCGAGTGCGTGCTG

GGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGGGCTACCACCTGA

TGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTGTTCCTGCACGT

GACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACCGCCCCCGCC

ATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGGCGTGTTCG

TGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTTCTACGA

GCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAACTGC

GACGTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCTGC

AGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATC

AACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACG

AGGTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCT

GGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTG

GGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCT

GTGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGC

TGCGGCAGCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTG

CTGAAGGGCGTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACT

TCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCT

ATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAAT

GCAGACACATTATGTATAGGTTATCATGCGAACAACTCAACAGACACT

GTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC

CTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGAGGGGT

AGCCCCATTGCATTTGGGTAAATGTAACATTGCTGGCTGGATCCTGG

GAAATCCAGAGTGTGAATCACTCTCCACAGCAAGCTCATGGTCCTAC

ATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAGGAGA

TTTCATCGATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTCAT

CATTTGAAAGGTTTGAGATATTCCCCAAGACAAGTTCATGGCCCAATC

ATGACTCGAACAAAGGTGTAACGACAGCATGTCCTCATGCTGGAGCA

AAAAGCTTCTACAAAAATTTAATATGGCTAGTTAAAAAAGGAAATTCAT

ACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGGGAAAGAAGTC

CTCGTGCTATGGGGCATTCACCATCCACCTACTAGTGCTGACCAACA

AAGTCTCTATCAGAATGCAGATGCATATGTTTTTGTGGGGACATCAAG

ATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGGCCCAAAGTGA

GGGATCAAGAAGGGAGAATGAACTATTACTGGACACTAGTAGAGCCG

GGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGTGGTACCGAG

ATATGCATTCGCAATGGAAAGAAATGCTGGATCTGGTATTATCATTTC

AGATACACCAGTCCACGATTGCAATACAACTTGTCAGACACCCAAGG

GTGCTATAAACACCAGCCTCCCATTTCAGAACATACATCCGATCACAA

TTGGAAAATGTCCAAAATATGTAAAAAGCACAAAATTGAGACTGGCCA

CAGGATTGAGGAATGTCCCGTCCATTCAATCTAGAGGCCTATTTGGG

GCCATTGCCGGTTTCATTGAAGGGGGGTGGACAGGGATGGTAGATG

GATGGTACGGTTATCACCATCAAAATGAGCAGGGGTCAGGATATGCA

GCCGACCTGAAGAGCACACAGAATGCCATTGACGAGATTACTAACAA

AGTAAACTCTGTTATTGAAAAAATGAATACACAGTTCACAGCAGTAGG

TAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGAATTTAAATAAAAA

AGTTGATGATGGTTTCCTGGACATTTGGACTTACAATGCCGAACTGTT

GGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAAGGT

GAAGAACTTATATGAAAAGGTAAGAAGCCAGTTAAAAAACAATGCCAA

GGAAATTGGAAACGGCTGCTTTGAATTTTACCACAAATGTGATAACAC

GTGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACT

CAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGGTAAAGCTG

GAATCAACAAGGATTTACCAGATTTTGGCGATCTATTCAACCGTCGCC

AGTTCATTGGTACTGGTAGTCTCCCTGGGGGCAATCAGTTTCTGGAT

GTGCTCTAATGGGTCTCTACAGTGTAGAATATGTATTTAAGGAAGCG

GAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAA

AACCCCGGGCCTAGCAAAAGCAGGGGATAATTCTATTAACCATGAAG

ACTATCATTGCTTTGAGCTACATTCTATGTCTGGTTTTCGCTCAAAAA

CTTCCTGGAAATGACAATAGCACTGCAACGCTGTGCCTTGGGCACCA

TGCAGTACCAAACGGAACGATAGTGAAAACAATCACGAATGACCAAA

TTGAAGTTACTAATGCTACTGAGCTGGTTCAGAATTCCTCAATAGGTG

AAATATGCGACAGTCCTCATCAGATCCTTGATGGAGAAAACTGCACA

CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAAT

AAGAAATGGGACCTTTTTGTTGAACGAAGCAAAGCCTACAGCAACTG

TTACCCTTATGATGTGCCGGATTATGCCTCCCTTAGGTCACTAGTTGC

CTCATCCGGCACACTGGAGTTTAACAATGAAAGCTTCAATTGGGCTG

GAGTCACTCAAAACGGAACAAGTTCTGCTTGCATAAGGGGATCTAAT

AGTAGTTTCTTTAGTAGATTAAATTGGTTGACCCACTTAAACTTCAAGT

ACCCAGCATTGAACGTGACTATGCCAAACAATGAACAATTTGACAAAT

TGTACATTTGGGGGGTTCACCACCCGGGTACGGACAAGGACCAAAT

CTTCCTGTATGCTCAATCATCAGGAAGAATCACAGTATCTACCAAAAG

AAGCCAACAAGCTGTAATCCCGAATATCGGATCTAGACCCAGAATAA

GGAATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAACCG

GGAGACATACTTTTGATTAACAGCACAGGGAATCTAATTGCTCCTAG

GGGTTACTTCAAAATACGAAGTGGGAAAAGCTCAATAATGAGATCAG

ATGCACCCATTGGCAAATGCAAGTCTGAATGCATCACTCCAAATGGA

AGCATTCCCAATGACAAACCATTCCAAAATGTAAACAGGATCACATAC

GGGGCCTGTCCCAGATATGTTAAGCAAAGCACTCTGAAATTGGCAAC

AGGAATGCGAAATGTACCAGAGAAACAAACTAGAGGCATATTTGGCG

CAATAGCGGGTTTCATAGAAAATGGTTGGGAGGGAATGGTGGATGGT

TGGTACGGTTTCAGGCATCAAAATTCTGAGGGAAGAGGACAAGCAGC

AGATCTCAAAAGCACTCAAGCAGCAATCGATCAAATCAATGGGAAGC

TGAATCGATTGATCGGGAAAACCAACGAGAAATTCCATCAGATTGAG

AAAGAATTCTCAGAAGTAGAAGGGAGAATTCAGGACCTTGAGAAATA

TGTTGAGGACACAAAAATAGATCTCTGGTCATACAACGCAGAGCTTC

TTGTTGCCCTGGAAAACCAACATACAATTGATCTAACTGACTCAGAAA

TGAACAAACTGTTTGAAAAAACAAAGAAGCAACTGAGGGAAAATGCT

GAGGATATGGGCAATGGTTGTTTCAAAATATACCACAAATGTGACAAT

GCCTGCATAGGATCAATCAGAAATGGAACTTATGACCACGATGTATA

CAGGGATGAAGCATTAAACAACCGGTTCCAGATCAAGGGAGTTGAGC

TGAAGTCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT

CATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCATCATGTGGGCCT

GCCAAAAGGGCAACATTAGGTGCAACATTTGCATTTGAGTGCATTAAT

TAAAAACACCCTTGTTTCTACTGGAAGCGGAGCCACGAACTTCTCTCT

GTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTATTTTCT

AATATCCACAAAATGAAGGCAATAATTGTACTACTCATGGTAGTAACA

TCCAATGCAGATCGAATCTGCACTGGGATAACATCGTCAAACTCACC

ACATGTCGTCAAAACTGCTACTCAAGGGGAGGTCAACGTGACCGGT

GTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC

AAAGGAACAGAAACCAGGGGGAAACTATGCCCAAAATGCCTCAACTG

CACAGATCTGGATGTAGCCTTGGGCAGACCAAAATGCACAGGGAAAA

TACCCTCTGCAAGGGTTTCAATACTCCATGAAGTCAGACCTGTTACAT

CTGGGTGCTTTCCTATAATGCACGATAGAACAAAAATTAGACAGCTG

CCTAACCTTCTCCGAGGATACGAACATGTCAGGTTATCAACTCACAA

CGTTATCAATGCAAAAGATGCACCAGGAAGACCCTACGAAATTGGAA

CCTCAGGGTCTTGCCCTAACATTACCAATGGAAACGGATTCTTCGCA

ACAATGGCTTGGGCCGTCCCAAAAAACAAAACAGCAACAAATCCATT

AACAATAGAAGTACCATACATTTGTACAGAAGGAGAAGACCAAATTAC

CGTTTGGGGGTTCCACTCTGACAACGAGACCCAAATGGCAAAGCTCT

ATGGGGACTCAAAGCCCCAGAAGTTCACCTCATCTGCCAACGGAGT

GACCACACATTACGTTTCACAGATTGGTGGCTTCCCAAATCAAACAG

AAGACGGAGGACTACCACAAAGTGGCAGAATTGTTGTTGATTACATG

GTGCAGAAATCTGGAAAAACAGGAACAATTACCTATCAAAGAGGTATT

TTATTGCCTCAAAAAGTGTGGTGCGCAAGTGGCAGGAGCAAGGTAAT

AAAAGGATCCTTGCCCTTAATTGGAGAAGCAGATTGCCTCCATGAAA

AATACGGTGGATTAAACAAAAGCAAGCCTTACTACACAGGGGAACAT

GCAAAGGCCATAGGAAATTGCCCAATATGGGTGAAAACACCCTTGAA

GCTGGCCAATGGAACCAAATATAGACCCCCTGCAAAACTATTAAAGG

AAAGAGGTTTCTTCGGAGCCATTGCTGGTTTCTTAGAGGGAGGATGG

GAAGGAATGATTGCAGGTTGGCACGGATACACATCCCATGGGGCAC

ATGGAGTAGCGGTGGCAGCTGACCTTAAGAGCACTCAAGAGGCCAT

AAACAAGATAACAAAAAATCTCAACTCTTTGAGTGAGCTGGAAGTAAA

GAATCTTCAAAGACTAAGCGGTGCCATGGATGAACTCCACAACGAAA

TACTAGAACTAGATGAGAAAGTGGATGATCTCAGAGCTGACACAATA

AGCTCACAAATAGAACTCGCAGTCCTGCTTTCCAATGAAGGAATAATA

AACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAAGAA

AATGCTGGGCCCCTCTGCTGTAGAGATAGGGAATGGATGCTTTGAAA

CCAAACACAAGTGCAACCAGACCTGCCTCGACAGAATAGCTGCTGGT

ACCTTTGATGCAGGAGAATTTTCTCTCCCCACCTTTGATTCACTGAAT

ATTACTGCTGCATCTTTAAATGACGACGGATTGGACAATCATACTATA

CTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACACTGATG

ATAGCTATCTTTGTTGTTTATATGGTCTCCAGAGACAATGTTTCTTGCT

CCATTTGTCTATAAGGAAAGTTAAGCCCTGTATTTTCCTTTATTGTAGT

GCTTGTTTGCTTGTTGTCATTACAAAGAAACGTTATTGAAAAATGGAA

GCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAA

GAAAACCCCGGGCCTTTCGGAGCTATTGCTGGTTTCTTGGAAGGAG

GATGGGAAGGAATGATTGCAGGTTGGCACGGATACACATCTCATGGA

GCACATGGAGTAGCAGTGGCAGCAGACCTTAAGAGTACCCATGATG

ACTCGAGCTGGTACTGCATGCACGCAATGCTAGCTGCCCCTTTCCCG

TCCTGGGTACCCCGAGTCTCCCCCGACCTCGGGTCCCAGGTATGCT

CCCACCTCCACCTGCCCCACTCACCACCTCTGCTAGTTCCAGACACC

TCCCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACAC

CCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT

TAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCA

CACCCTGGAGCTAGCAAAAAAAA

Pan-CoV-Flu
CTCGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGT
401

Vaccine #13
CATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGG

CoV-Wuhan
TAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGAC

Spike(HexaPro)-
GTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCA

Influenza (HA)
TTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAG

TACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATG

ACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGG

GACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACC

ATGGTCGAGGTGAGCCCCACGTTCTGCTTCACTCTCCCCATCTCCCC

CCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTTTGT

GCAGCGATGGGGGCGGGGGGGGGGGGGGGGCGCGCGCCAGGCG

GGGCGGGGCGGGGCGAGGGGCGGGGCGGGGCGAGGCGGAGAGG

TGCGGCGGCAGCCAATCAGAGCGGCGCGCTCCGAAAGTTTCCTTTT

ATGGCGAGGCGGCGGCGGCGGCGGCCCTATAAAAAGCGAAGCGCG

CGGCGGGCGGGAGTCGCTGCGCGCTGCCTTCGCCCCGTGCCCCGC

TCCGCCGCCGCCTCGCGCCGCCCGCCCCGGCTCTGACTGACCGCG

TTACTCCCACAGGTGAGCGGGCGGGACGGCCCTTCTCCTCCGGGCT

GTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGC

GTGAAAGCCTTGAGGGGCTCCGGGAGGGCCCTTTGTGCGGGGGGA

GCGGCTCGGGGGGTGCGTGCGTGTGTGTGTGCGTGGGGAGCGCCG

CGTGCGGCTCCGCGCTGCCCGGCGGCTGTGAGCGCTGCGGGCGCG

GCGCGGGGCTTTGTGCGCTCCGCAGTGTGCGCGAGGGGAGCGCGG

CCGGGGGCGGTGCCCCGCGGTGCGGGGGGGGCTGCGAGGGGAAC

AAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGGGTGAGCAGGGGGT

GTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTGCACCCCCCTCCC

CGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGGGCTCCGTACG

GGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGGTGGCGGCA

GGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCCGGGGAG

GGCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGCGGCTG

TCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCGTGC

GAGAGGGCGCAGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCGA

AATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGA

AGCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTC

GTGCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGG

CTGTCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGG

CGGGGTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGC

TAACCATGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGT

GCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAGAATTGGAGAATAA

ACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGAACCCGCCACCA

TGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGT

GAACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAGC

TTCACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCG

TGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTG

ACCTGGTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAGA

GGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGC

CAGCACCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACC

ACCCTGGACAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCA

CCAACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCC

CTTCCTGGGCGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGA

GCGAGTTCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTA

CGTGAGCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAAC

TTCAAGAACCTGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTT

CAAGATCTACAGCAAGCACACCCCCATCAACCTGGTGAGGGACCTG

CCCCAGGGCTTCAGCGCCCTGGAGCCCCTGGTGGACCTGCCCATCG

GCATCAACATCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAG

CTACCTGACCCCCGGCGACAGCAGCAGCGGCTGGACCGCCGGCGC

CGCCGCCTACTACGTGGGCTACCTGCAGCCCAGGACCTTCCTGCTG

AAGTACAACGAGAACGGCACCATCACCGACGCCGTGGACTGCGCCC

TGGACCCCCTGAGCGAGACCAAGTGCACCCTGAAGAGCTTCACCGT

GGAGAAGGGCATCTACCAGACCAGCAACTTCAGGGTGCAGCCCACC

GAGAGCATCGTGAGGTTCCCCAACATCACCAACCTGTGCCCCTTCG

GCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGTACGCCTGGAA

CAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTAC

AACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTGAGCCCCA

CCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTC

GTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCAGACC

GGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCACCG

GCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGGG

CGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAG

CACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTG

CAGAGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCT

ACAGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCAC

CGTGTGCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGC

GTGAACTTCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCG

AGAGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACAT

CGCCGACACCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGAT

CCTGGACATCACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACC

CCCGGCACCAACACCAGCAACCAGGTGGCCGTGCTGTACCAGGACG

TGAACTGCACCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGAC

CCCCACCTGGAGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACC

AGGGCCGGCTGCCTGATCGGCGCCGAGCACGTGAACAACAGCTAC

GAGTGCGACATCCCCATCGGCGCCGGCATCTGCGCCAGCTACCAGA

CCCAGACCAACAGCCCCGGCAGCGCCAGCAGCGTGGCCAGCCAGA

GCATCATCGCCTACACCATGAGCCTGGGCGCCGAGAACAGCGTGGC

CTACAGCAACAACAGCATCGCCATCCCCACCAACTTCACCATCAGCG

TGACCACCGAGATCCTGCCCGTGAGCATGACCAAGACCAGCGTGGA

CTGCACCATGTACATCTGCGGCGACAGCACCGAGTGCAGCAACCTG

CTGCTGCAGTACGGCAGCTTCTGCACCCAGCTGAACAGGGCCCTGA

CCGGCATCGCCGTGGAGCAGGACAAGAACACCCAGGAGGTGTTCGC

CCAGGTGAAGCAGATCTACAAGACCCCCCCCATCAAGGACTTCGGC

GGCTTCAACTTCAGCCAGATCCTGCCCGACCCCAGCAAGCCCAGCA

AGAGGAGCCCCATCGAGGACCTGCTGTTCAACAAGGTGACCCTGGC

CGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCTGGGCGACATC

GCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACGGCCTGACCG

TGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAGTACACCAG

CGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTTCGGCGC

CGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATGGCCTACAGG

TTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACCAGA

AGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGGA

CAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGT

GGTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTG

AGCAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGA

GCAGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGA

TCACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCT

GATCAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCAC

CAAGATGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTC

TGCGGCAAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCC

ACGGCGTGGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAA

GAACTTCACCACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCAC

TTCCCCAGGGAGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCG

TGACCCAGAGGAACTTCTACGAGCCCCAGATCATCACCACCGACAAC

ACCTTCGTGAGCGGCAACTGCGACGTGGTGATCGGCATCGTGAACA

ACACCGTGTACGACCCCCTGCAGCCCGAGCTGGACAGCTTCAAGGA

GGAGCTGGACAAGTACTTCAAGAACCACACCAGCCCCGACGTGGAC

CTGGGCGACATCAGCGGCATCAACGCCAGCGTGGTGAACATCCAGA

AGGAGATCGACAGGCTGAACGAGGTGGCCAAGAACCTGAACGAGAG

CCTGATCGACCTGCAGGAGCTGGGCAAGTACGAGCAGTACATCAAG

TGGCCCTGGTACATCTGGCTGGGCTTCATCGCCGGCCTGATCGCCA

TCGTGATGGTGACCATCATGCTGTGCTGCATGACCAGCTGCTGCAGC

TGCCTGAAGGGCTGCTGCAGCTGCGGCAGCTGCTGCAAGTTCGACG

AGGACGACAGCGAGCCCGTGCTGAAGGGCGTGAAGCTGCACTACAC

CGGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGAT

GTTGAAGAAAACCCCGGGCCTATGAAGGCAATACTAGTAGTTCTGCT

ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCA

TGCGAACAACTCAACAGACACTGTAGACACAGTACTAGAAAAGAATG

TAACAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGA

AACTATGCAAACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGT

AACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCTC

CACAGCAAGCTCATGGTCCTACATTGTGGAAACATCTAGTTCAGACA

ATGGAACGTGTTACCCAGGAGATTTCATCGATTATGAGGAGCTAAGA

GAGCAATTGAGCTCAGTGTCATCATTTGAAAGGTTTGAGATATTCCCC

AAGACAAGTTCATGGCCCAATCATGACTCGAACAAAGGTGTAACGAC

AGCATGTCCTCATGCTGGAGCAAAAAGCTTCTACAAAAATTTAATATG

GCTAGTTAAAAAAGGAAATTCATACCCAAAGCTCAGCAAATCCTACAT

TAATGATAAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTCACCATC

CACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCA

TATGTTTTTGTGGGGACATCAAGATACAGCAAGAAGTTCAAGCCGGA

AATAGCAATAAGGCCCAAAGTGAGGGATCAAGAAGGGAGAATGAACT

ATTACTGGACACTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCA

ACTGGAAATCTAGTGGTACCGAGATATGCATTCGCAATGGAAAGAAA

TGCTGGATCTGGTATTATCATTTCAGATACACCAGTCCACGATTGCAA

TACAACTTGTCAGACACCCAAGGGTGCTATAAACACCAGCCTCCCAT

TTCAGAACATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAA

AAAGCACAAAATTGAGACTGGCCACAGGATTGAGGAATGTCCCGTCC

ATTCAATCTAGAGGCCTATTTGGGGCCATTGCCGGTTTCATTGAAGG

GGGGTGGACAGGGATGGTAGATGGATGGTACGGTTATCACCATCAA

AATGAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACACAGA

ATGCCATTGACGAGATTACTAACAAAGTAAACTCTGTTATTGAAAAAA

TGAATACACAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAA

AAAAGAATAGAGAATTTAAATAAAAAAGTTGATGATGGTTTCCTGGAC

ATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGA

ACTTTGGACTACCACGATTCAAAGGTGAAGAACTTATATGAAAAGGTA

AGAAGCCAGTTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTT

TGAATTTTACCACAAATGTGATAACACGTGCATGGAAAGTGTCAAAAA

TGGGACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACA

GAGAAGAAATAGATGGGGTAAAGCTGGAATCAACAAGGATTTACCAG

ATTTTGGCGATCTATTCAACCGTCGCCAGTTCATTGGTACTGGTAGTC

TCCCTGGGGGCAATCAGTTTCTGGATGTGCTCTAATGGGTCTCTACA

GTGTAGAATATGTATTTAAGGAAGCGGAGCCACGAACTTCTCTCTGTT

AAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTAGCAAAAGC

AGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTTGAGCTACA

TTCTATGTCTGGTTTTCGCTCAAAAACTTCCTGGAAATGACAATAGCA

CTGCAACGCTGTGCCTTGGGCACCATGCAGTACCAAACGGAACGAT

AGTGAAAACAATCACGAATGACCAAATTGAAGTTACTAATGCTACTGA

GCTGGTTCAGAATTCCTCAATAGGTGAAATATGCGACAGTCCTCATC

AGATCCTTGATGGAGAAAACTGCACACTAATAGATGCTCTATTGGGA

GACCCTCAGTGTGATGGCTTTCAAAATAAGAAATGGGACCTTTTTGTT

GAACGAAGCAAAGCCTACAGCAACTGTTACCCTTATGATGTGCCGGA

TTATGCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGT

TTAACAATGAAAGCTTCAATTGGGCTGGAGTCACTCAAAACGGAACA

AGTTCTGCTTGCATAAGGGGATCTAATAGTAGTTTCTTTAGTAGATTA

AATTGGTTGACCCACTTAAACTTCAAGTACCCAGCATTGAACGTGACT

ATGCCAAACAATGAACAATTTGACAAATTGTACATTTGGGGGGTTCAC

CACCCGGGTACGGACAAGGACCAAATCTTCCTGTATGCTCAATCATC

AGGAAGAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCC

CGAATATCGGATCTAGACCCAGAATAAGGAATATCCCTAGCAGAATA

AGCATCTATTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAAC

AGCACAGGGAATCTAATTGCTCCTAGGGGTTACTTCAAAATACGAAG

TGGGAAAAGCTCAATAATGAGATCAGATGCACCCATTGGCAAATGCA

AGTCTGAATGCATCACTCCAAATGGAAGCATTCCCAATGACAAACCAT

TCCAAAATGTAAACAGGATCACATACGGGGCCTGTCCCAGATATGTT

AAGCAAAGCACTCTGAAATTGGCAACAGGAATGCGAAATGTACCAGA

GAAACAAACTAGAGGCATATTTGGCGCAATAGCGGGTTTCATAGAAA

ATGGTTGGGAGGGAATGGTGGATGGTTGGTACGGTTTCAGGCATCA

AAATTCTGAGGGAAGAGGACAAGCAGCAGATCTCAAAAGCACTCAAG

CAGCAATCGATCAAATCAATGGGAAGCTGAATCGATTGATCGGGAAA

ACCAACGAGAAATTCCATCAGATTGAGAAAGAATTCTCAGAAGTAGAA

GGGAGAATTCAGGACCTTGAGAAATATGTTGAGGACACAAAAATAGA

TCTCTGGTCATACAACGCAGAGCTTCTTGTTGCCCTGGAAAACCAAC

ATACAATTGATCTAACTGACTCAGAAATGAACAAACTGTTTGAAAAAA

CAAAGAAGCAACTGAGGGAAAATGCTGAGGATATGGGCAATGGTTGT

TTCAAAATATACCACAAATGTGACAATGCCTGCATAGGATCAATCAGA

AATGGAACTTATGACCACGATGTATACAGGGATGAAGCATTAAACAA

CCGGTTCCAGATCAAGGGAGTTGAGCTGAAGTCAGGGTACAAAGATT

GGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGC

TTTGTTGGGGTTCATCATGTGGGCCTGCCAAAAGGGCAACATTAGGT

GCAACATTTGCATTTGAGTGCATTAATTAAAAACACCCTTGTTTCTACT

GGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGT

TGAAGAAAACCCCGGGCCTATTTTCTAATATCCACAAAATGAAGGCAA

TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCA

CTGGGATAACATCGTCAAACTCACCACATGTCGTCAAAACTGCTACT

CAAGGGGAGGTCAACGTGACCGGTGTAATACCACTGACAACAACAC

CCACCAAATCTCATTTTGCAAATCTCAAAGGAACAGAAACCAGGGGG

AAACTATGCCCAAAATGCCTCAACTGCACAGATCTGGATGTAGCCTT

GGGCAGACCAAAATGCACAGGGAAAATACCCTCTGCAAGGGTTTCAA

TACTCCATGAAGTCAGACCTGTTACATCTGGGTGCTTTCCTATAATGC

ACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTCCGAGGATAC

GAACATGTCAGGTTATCAACTCACAACGTTATCAATGCAAAAGATGCA

CCAGGAAGACCCTACGAAATTGGAACCTCAGGGTCTTGCCCTAACAT

TACCAATGGAAACGGATTCTTCGCAACAATGGCTTGGGCCGTCCCAA

AAAACAAAACAGCAACAAATCCATTAACAATAGAAGTACCATACATTT

GTACAGAAGGAGAAGACCAAATTACCGTTTGGGGGTTCCACTCTGAC

AACGAGACCCAAATGGCAAAGCTCTATGGGGACTCAAAGCCCCAGA

AGTTCACCTCATCTGCCAACGGAGTGACCACACATTACGTTTCACAG

ATTGGTGGCTTCCCAAATCAAACAGAAGACGGAGGACTACCACAAAG

TGGCAGAATTGTTGTTGATTACATGGTGCAGAAATCTGGAAAAACAG

GAACAATTACCTATCAAAGAGGTATTTTATTGCCTCAAAAAGTGTGGT

GCGCAAGTGGCAGGAGCAAGGTAATAAAAGGATCCTTGCCCTTAATT

GGAGAAGCAGATTGCCTCCATGAAAAATACGGTGGATTAAACAAAAG

CAAGCCTTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCC

CAATATGGGTGAAAACACCCTTGAAGCTGGCCAATGGAACCAAATAT

AGACCCCCTGCAAAACTATTAAAGGAAAGAGGTTTCTTCGGAGCCAT

TGCTGGTTTCTTAGAGGGAGGATGGGAAGGAATGATTGCAGGTTGG

CACGGATACACATCCCATGGGGCACATGGAGTAGCGGTGGCAGCTG

ACCTTAAGAGCACTCAAGAGGCCATAAACAAGATAACAAAAAATCTCA

ACTCTTTGAGTGAGCTGGAAGTAAAGAATCTTCAAAGACTAAGCGGT

GCCATGGATGAACTCCACAACGAAATACTAGAACTAGATGAGAAAGT

GGATGATCTCAGAGCTGACACAATAAGCTCACAAATAGAACTCGCAG

TCCTGCTTTCCAATGAAGGAATAATAAACAGTGAAGATGAACATCTCT

TGGCGCTTGAAAGAAAGCTGAAGAAAATGCTGGGCCCCTCTGCTGTA

GAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCAGAC

CTGCCTCGACAGAATAGCTGCTGGTACCTTTGATGCAGGAGAATTTT

CTCTCCCCACCTTTGATTCACTGAATATTACTGCTGCATCTTTAAATG

ACGACGGATTGGACAATCATACTATACTGCTTTACTACTCAACTGCTG

CCTCCAGTTTGGCTGTAACACTGATGATAGCTATCTTTGTTGTTTATA

TGGTCTCCAGAGACAATGTTTCTTGCTCCATTTGTCTATAAGGAAAGT

TAAGCCCTGTATTTTCCTTTATTGTAGTGCTTGTTTGCTTGTTGTCATT

ACAAAGAAACGTTATTGAAAAATGGAAGCGGAGCCACGAACTTCTCT

CTGTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTTTCGG

AGCTATTGCTGGTTTCTTGGAAGGAGGATGGGAAGGAATGATTGCAG

GTTGGCACGGATACACATCTCATGGAGCACATGGAGTAGCAGTGGC

AGCAGACCTTAAGAGTACCCATGATGACTCGAGCTGGTACTGCATGC

ACGCAATGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCCGAGTCTCC

CCCGACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGCCCCACT

CACCACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCAATGCA

GCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTG

ATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCC

AGGGTTGGTCAATTTCGTGCCAGCCACACCCTGGAGCTAGCAAAAAA

AA

Pan-CoV-Flu
CTCGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGT
402

Vaccine #14
CATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGG

CoV-Wuhan
TAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGAC

Spike(2 proline
GTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCA

substitution)-
TTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAG

influenza (HA)
TACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATG

ACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGG

GACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACC

ATGGTCGAGGTGAGCCCCACGTTCTGCTTCACTCTCCCCATCTCCCC

CCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTTTGT

GCAGCGATGGGGGCGGGGGGGGGGGGGGGGCGCGCGCCAGGCG

GGGCGGGGCGGGGCGAGGGGCGGGGCGGGGCGAGGCGGAGAGG

TGCGGCGGCAGCCAATCAGAGCGGCGCGCTCCGAAAGTTTCCTTTT

ATGGCGAGGCGGCGGCGGCGGCGGCCCTATAAAAAGCGAAGCGCG

CGGCGGGCGGGAGTCGCTGCGCGCTGCCTTCGCCCCGTGCCCCGC

TCCGCCGCCGCCTCGCGCCGCCCGCCCCGGCTCTGACTGACCGCG

TTACTCCCACAGGTGAGCGGGCGGGACGGCCCTTCTCCTCCGGGCT

GTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGC

GTGAAAGCCTTGAGGGGCTCCGGGAGGGCCCTTTGTGCGGGGGGA

GCGGCTCGGGGGGTGCGTGCGTGTGTGTGTGCGTGGGGAGCGCCG

CGTGCGGCTCCGCGCTGCCCGGCGGCTGTGAGCGCTGCGGGCGCG

GCGCGGGGCTTTGTGCGCTCCGCAGTGTGCGCGAGGGGAGCGCGG

CCGGGGGCGGTGCCCCGCGGTGCGGGGGGGGCTGCGAGGGGAAC

AAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGGGTGAGCAGGGGGT

GTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTGCACCCCCCTCCC

CGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGGGCTCCGTACG

GGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGGTGGCGGCA

GGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCCGGGGAG

GGCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGCGGCTG

TCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCGTGC

GAGAGGGCGCAGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCGA

AATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGA

AGCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTC

GTGCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGG

CTGTCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGG

CGGGGTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGC

TAACCATGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGT

GCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAGAATTGGAGAATAA

ACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGAACCCGCCACCA

TGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGT

GAACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAGC

TTCACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCG

TGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTG

ACCTGGTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAGA

GGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGC

CAGCACCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACC

ACCCTGGACAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCA

CCAACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCC

CTTCCTGGGCGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGA

GCGAGTTCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTA

CGTGAGCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAAC

TTCAAGAACCTGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTT

CAAGATCTACAGCAAGCACACCCCCATCAACCTGGTGAGGGACCTG

CCCCAGGGCTTCAGCGCCCTGGAGCCCCTGGTGGACCTGCCCATCG

GCATCAACATCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAG

CTACCTGACCCCCGGCGACAGCAGCAGCGGCTGGACCGCCGGCGC

CGCCGCCTACTACGTGGGCTACCTGCAGCCCAGGACCTTCCTGCTG

AAGTACAACGAGAACGGCACCATCACCGACGCCGTGGACTGCGCCC

TGGACCCCCTGAGCGAGACCAAGTGCACCCTGAAGAGCTTCACCGT

GGAGAAGGGCATCTACCAGACCAGCAACTTCAGGGTGCAGCCCACC

GAGAGCATCGTGAGGTTCCCCAACATCACCAACCTGTGCCCCTTCG

GCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGTACGCCTGGAA

CAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTAC

AACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTGAGCCCCA

CCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTC

GTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCAGACC

GGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCACCG

GCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGGG

CGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAG

CACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTG

CAGAGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCT

ACAGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCAC

CGTGTGCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGC

GTGAACTTCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCG

AGAGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACAT

CGCCGACACCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGAT

CCTGGACATCACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACC

CCCGGCACCAACACCAGCAACCAGGTGGCCGTGCTGTACCAGGACG

TGAACTGCACCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGAC

CCCCACCTGGAGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACC

AGGGCCGGCTGCCTGATCGGCGCCGAGCACGTGAACAACAGCTAC

GAGTGCGACATCCCCATCGGCGCCGGCATCTGCGCCAGCTACCAGA

CCCAGACCAACAGCCCCAGGAGGGCCAGGAGCGTGGCCAGCCAGA

GCATCATCGCCTACACCATGAGCCTGGGCGCCGAGAACAGCGTGGC

CTACAGCAACAACAGCATCGCCATCCCCACCAACTTCACCATCAGCG

TGACCACCGAGATCCTGCCCGTGAGCATGACCAAGACCAGCGTGGA

CTGCACCATGTACATCTGCGGCGACAGCACCGAGTGCAGCAACCTG

CTGCTGCAGTACGGCAGCTTCTGCACCCAGCTGAACAGGGCCCTGA

CCGGCATCGCCGTGGAGCAGGACAAGAACACCCAGGAGGTGTTCGC

CCAGGTGAAGCAGATCTACAAGACCCCCCCCATCAAGGACTTCGGC

GGCTTCAACTTCAGCCAGATCCTGCCCGACCCCAGCAAGCCCAGCA

AGAGGAGCTTCATCGAGGACCTGCTGTTCAACAAGGTGACCCTGGC

CGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCTGGGCGACATC

GCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACGGCCTGACCG

TGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAGTACACCAG

CGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTTCGGCGC

CGGCGCCGCCCTGCAGATCCCCTTCGCCATGCAGATGGCCTACAGG

TTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACCAGA

AGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGGA

CAGCCTGAGCAGCACCGCCAGCGCCCTGGGCAAGCTGCAGGACGT

GGTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTG

AGCAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGA

GCAGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGA

TCACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCT

GATCAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCAC

CAAGATGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTC

TGCGGCAAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCC

ACGGCGTGGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAA

GAACTTCACCACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCAC

TTCCCCAGGGAGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCG

TGACCCAGAGGAACTTCTACGAGCCCCAGATCATCACCACCGACAAC

ACCTTCGTGAGCGGCAACTGCGACGTGGTGATCGGCATCGTGAACA

ACACCGTGTACGACCCCCTGCAGCCCGAGCTGGACAGCTTCAAGGA

GGAGCTGGACAAGTACTTCAAGAACCACACCAGCCCCGACGTGGAC

CTGGGCGACATCAGCGGCATCAACGCCAGCGTGGTGAACATCCAGA

AGGAGATCGACAGGCTGAACGAGGTGGCCAAGAACCTGAACGAGAG

CCTGATCGACCTGCAGGAGCTGGGCAAGTACGAGCAGTACATCAAG

TGGCCCTGGTACATCTGGCTGGGCTTCATCGCCGGCCTGATCGCCA

TCGTGATGGTGACCATCATGCTGTGCTGCATGACCAGCTGCTGCAGC

TGCCTGAAGGGCTGCTGCAGCTGCGGCAGCTGCTGCAAGTTCGACG

AGGACGACAGCGAGCCCGTGCTGAAGGGCGTGAAGCTGCACTACAC

CGGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGAT

GTTGAAGAAAACCCCGGGCCTATGAAGGCAATACTAGTAGTTCTGCT

ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCA

TGCGAACAACTCAACAGACACTGTAGACACAGTACTAGAAAAGAATG

TAACAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGA

AACTATGCAAACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGT

AACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCTC

CACAGCAAGCTCATGGTCCTACATTGTGGAAACATCTAGTTCAGACA

ATGGAACGTGTTACCCAGGAGATTTCATCGATTATGAGGAGCTAAGA

GAGCAATTGAGCTCAGTGTCATCATTTGAAAGGTTTGAGATATTCCCC

AAGACAAGTTCATGGCCCAATCATGACTCGAACAAAGGTGTAACGAC

AGCATGTCCTCATGCTGGAGCAAAAAGCTTCTACAAAAATTTAATATG

GCTAGTTAAAAAAGGAAATTCATACCCAAAGCTCAGCAAATCCTACAT

TAATGATAAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTCACCATC

CACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCA

TATGTTTTTGTGGGGACATCAAGATACAGCAAGAAGTTCAAGCCGGA

AATAGCAATAAGGCCCAAAGTGAGGGATCAAGAAGGGAGAATGAACT

ATTACTGGACACTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCA

ACTGGAAATCTAGTGGTACCGAGATATGCATTCGCAATGGAAAGAAA

TGCTGGATCTGGTATTATCATTTCAGATACACCAGTCCACGATTGCAA

TACAACTTGTCAGACACCCAAGGGTGCTATAAACACCAGCCTCCCAT

TTCAGAACATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAA

AAAGCACAAAATTGAGACTGGCCACAGGATTGAGGAATGTCCCGTCC

ATTCAATCTAGAGGCCTATTTGGGGCCATTGCCGGTTTCATTGAAGG

GGGGTGGACAGGGATGGTAGATGGATGGTACGGTTATCACCATCAA

AATGAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACACAGA

ATGCCATTGACGAGATTACTAACAAAGTAAACTCTGTTATTGAAAAAA

TGAATACACAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAA

AAAAGAATAGAGAATTTAAATAAAAAAGTTGATGATGGTTTCCTGGAC

ATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGA

ACTTTGGACTACCACGATTCAAAGGTGAAGAACTTATATGAAAAGGTA

AGAAGCCAGTTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTT

TGAATTTTACCACAAATGTGATAACACGTGCATGGAAAGTGTCAAAAA

TGGGACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACA

GAGAAGAAATAGATGGGGTAAAGCTGGAATCAACAAGGATTTACCAG

ATTTTGGCGATCTATTCAACCGTCGCCAGTTCATTGGTACTGGTAGTC

TCCCTGGGGGCAATCAGTTTCTGGATGTGCTCTAATGGGTCTCTACA

GTGTAGAATATGTATTTAAGGAAGCGGAGCCACGAACTTCTCTCTGTT

AAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTAGCAAAAGC

AGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTTGAGCTACA

TTCTATGTCTGGTTTTCGCTCAAAAACTTCCTGGAAATGACAATAGCA

CTGCAACGCTGTGCCTTGGGCACCATGCAGTACCAAACGGAACGAT

AGTGAAAACAATCACGAATGACCAAATTGAAGTTACTAATGCTACTGA

GCTGGTTCAGAATTCCTCAATAGGTGAAATATGCGACAGTCCTCATC

AGATCCTTGATGGAGAAAACTGCACACTAATAGATGCTCTATTGGGA

GACCCTCAGTGTGATGGCTTTCAAAATAAGAAATGGGACCTTTTTGTT

GAACGAAGCAAAGCCTACAGCAACTGTTACCCTTATGATGTGCCGGA

TTATGCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGT

TTAACAATGAAAGCTTCAATTGGGCTGGAGTCACTCAAAACGGAACA

AGTTCTGCTTGCATAAGGGGATCTAATAGTAGTTTCTTTAGTAGATTA

AATTGGTTGACCCACTTAAACTTCAAGTACCCAGCATTGAACGTGACT

ATGCCAAACAATGAACAATTTGACAAATTGTACATTTGGGGGGTTCAC

CACCCGGGTACGGACAAGGACCAAATCTTCCTGTATGCTCAATCATC

AGGAAGAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCC

CGAATATCGGATCTAGACCCAGAATAAGGAATATCCCTAGCAGAATA

AGCATCTATTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAAC

AGCACAGGGAATCTAATTGCTCCTAGGGGTTACTTCAAAATACGAAG

TGGGAAAAGCTCAATAATGAGATCAGATGCACCCATTGGCAAATGCA

AGTCTGAATGCATCACTCCAAATGGAAGCATTCCCAATGACAAACCAT

TCCAAAATGTAAACAGGATCACATACGGGGCCTGTCCCAGATATGTT

AAGCAAAGCACTCTGAAATTGGCAACAGGAATGCGAAATGTACCAGA

GAAACAAACTAGAGGCATATTTGGCGCAATAGCGGGTTTCATAGAAA

ATGGTTGGGAGGGAATGGTGGATGGTTGGTACGGTTTCAGGCATCA

AAATTCTGAGGGAAGAGGACAAGCAGCAGATCTCAAAAGCACTCAAG

CAGCAATCGATCAAATCAATGGGAAGCTGAATCGATTGATCGGGAAA

ACCAACGAGAAATTCCATCAGATTGAGAAAGAATTCTCAGAAGTAGAA

GGGAGAATTCAGGACCTTGAGAAATATGTTGAGGACACAAAAATAGA

TCTCTGGTCATACAACGCAGAGCTTCTTGTTGCCCTGGAAAACCAAC

ATACAATTGATCTAACTGACTCAGAAATGAACAAACTGTTTGAAAAAA

CAAAGAAGCAACTGAGGGAAAATGCTGAGGATATGGGCAATGGTTGT

TTCAAAATATACCACAAATGTGACAATGCCTGCATAGGATCAATCAGA

AATGGAACTTATGACCACGATGTATACAGGGATGAAGCATTAAACAA

CCGGTTCCAGATCAAGGGAGTTGAGCTGAAGTCAGGGTACAAAGATT

GGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGC

TTTGTTGGGGTTCATCATGTGGGCCTGCCAAAAGGGCAACATTAGGT

GCAACATTTGCATTTGAGTGCATTAATTAAAAACACCCTTGTTTCTACT

GGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGT

TGAAGAAAACCCCGGGCCTATTTTGTAATATCCACAAAATGAAGGCAA

TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCA

CTGGGATAACATCGTCAAACTCACCACATGTCGTCAAAACTGCTACT

CAAGGGGAGGTCAACGTGACCGGTGTAATACCACTGACAACAACAC

CCACCAAATCTCATTTTGCAAATCTCAAAGGAACAGAAACCAGGGGG

AAACTATGCCCAAAATGCCTCAACTGCACAGATCTGGATGTAGCCTT

GGGCAGACCAAAATGCACAGGGAAAATACCCTCTGCAAGGGTTTCAA

TACTCCATGAAGTCAGACCTGTTACATCTGGGTGCTTTCCTATAATGC

ACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTCCGAGGATAC

GAACATGTCAGGTTATCAACTCACAACGTTATCAATGCAAAAGATGCA

CCAGGAAGACCCTACGAAATTGGAACCTCAGGGTCTTGCCCTAACAT

TACCAATGGAAACGGATTCTTCGCAACAATGGCTTGGGCCGTCCCAA

AAAACAAAACAGCAACAAATCCATTAACAATAGAAGTACCATACATTT

GTACAGAAGGAGAAGACCAAATTACCGTTTGGGGGTTCCACTCTGAC

AACGAGACCCAAATGGCAAAGCTCTATGGGGACTCAAAGCCCCAGA

AGTTCACCTCATCTGCCAACGGAGTGACCACACATTACGTTTCACAG

ATTGGTGGCTTCCCAAATCAAACAGAAGACGGAGGACTACCACAAAG

TGGCAGAATTGTTGTTGATTACATGGTGCAGAAATCTGGAAAAACAG

GAACAATTACCTATCAAAGAGGTATTTTATTGCCTCAAAAAGTGTGGT

GCGCAAGTGGCAGGAGCAAGGTAATAAAAGGATCCTTGCCCTTAATT

GGAGAAGCAGATTGCCTCCATGAAAAATACGGTGGATTAAACAAAAG

CAAGCCTTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCC

CAATATGGGTGAAAACACCCTTGAAGCTGGCCAATGGAACCAAATAT

AGACCCCCTGCAAAACTATTAAAGGAAAGAGGTTTCTTCGGAGCCAT

TGCTGGTTTCTTAGAGGGAGGATGGGAAGGAATGATTGCAGGTTGG

CACGGATACACATCCCATGGGGCACATGGAGTAGCGGTGGCAGCTG

ACCTTAAGAGCACTCAAGAGGCCATAAACAAGATAACAAAAAATCTCA

ACTCTTTGAGTGAGCTGGAAGTAAAGAATCTTCAAAGACTAAGCGGT

GCCATGGATGAACTCCACAACGAAATACTAGAACTAGATGAGAAAGT

GGATGATCTCAGAGCTGACACAATAAGCTCACAAATAGAACTCGCAG

TCCTGCTTTCCAATGAAGGAATAATAAACAGTGAAGATGAACATCTCT

TGGCGCTTGAAAGAAAGCTGAAGAAAATGCTGGGCCCCTCTGCTGTA

GAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCAGAC

CTGCCTCGACAGAATAGCTGCTGGTACCTTTGATGCAGGAGAATTTT

CTCTCCCCACCTTTGATTCACTGAATATTACTGCTGCATCTTTAAATG

ACGACGGATTGGACAATCATACTATACTGCTTTACTACTCAACTGCTG

CCTCCAGTTTGGCTGTAACACTGATGATAGCTATCTTTGTTGTTTATA

TGGTCTCCAGAGACAATGTTTCTTGCTCCATTTGTCTATAAGGAAAGT

TAAGCCCTGTATTTTCCTTTATTGTAGTGCTTGTTTGCTTGTTGTCATT

ACAAAGAAACGTTATTGAAAAATGGAAGCGGAGCCACGAACTTCTCT

CTGTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTTTCGG

AGCTATTGCTGGTTTCTTGGAAGGAGGATGGGAAGGAATGATTGCAG

GTTGGCACGGATACACATCTCATGGAGCACATGGAGTAGCAGTGGC

AGCAGACCTTAAGAGTACCCATGATGACTCGAGCTGGTACTGCATGC

ACGCAATGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCCGAGTCTCC

CCCGACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGCCCCACT

CACCACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCAATGCA

GCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTG

ATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCC

AGGGTTGGTCAATTTCGTGCCAGCCACACCCTGGAGCTAGCAAAAAA

AA

Pan-CoV-Flu
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATAT
403

Vaccine #15
ATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTG

CoV- Omicron
ACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTC

BA.2
CCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAG

Spike(HexaPro)-
TATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATG

Influenza (HA)
CCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTG

GCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTA

CATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCA

GTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAA

GTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAAT

CAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCA

AATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT

GGTTTAGTGAACCGTCAGATCGAGAATAAACTAGTATTCTTCTGGTCC

CCACAGACTCAGAGAGAACCCGCCACCATGTTCGTGTTCCTGGTGCT

GCTGCCCCTGGTGAGCAGCCAGTGCGTGAACCTGATCACCAGGACC

CAGAGCTACACCAACAGCTTCACCAGGGGCGTGTACTACCCCGACA

AGGTGTTCAGGAGCAGCGTGCTGCACAGCACCCAGGACCTGTTCCT

GCCCTTCTTCAGCAACGTGACCTGGTTCCACGCCATCCACGTGAGC

GGCACCAACGGCACCAAGAGGTTCGACAACCCCGTGCTGCCCTTCA

ACGACGGCGTGTACTTCGCCAGCACCGAGAAGAGCAACATCATCAG

GGGCTGGATCTTCGGCACCACCCTGGACAGCAAGACCCAGAGCCTG

CTGATCGTGAACAACGCCACCAACGTGGTGATCAAGGTGTGCGAGTT

CCAGTTCTGCAACGACCCCTTCCTGGACGTGTACTACCACAAGAACA

ACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTACAGCAGCGCCAA

CAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCTGATGGACCTG

GAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGTTCGTGTTCA

AGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACACCCCCATC

AACCTGGGCAGGGACCTGCCCCAGGGCTTCAGCGCCCTGGAGCCC

CTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGACCCT

GCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGCAG

CGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCA

GCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACC

GACGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGC

ACCCTGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAGCA

ACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACAT

CACCAACCTGTGCCCCTTCGACGAGGTGTTCAACGCCACCAGGTTC

GCCAGCGTGTACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGG

CCGACTACAGCGTGCTGTACAACTTCGCCCCCTTCTTCGCCTTCAAG

TGCTACGGCGTGAGCCCCACCAAGCTGAACGACCTGTGCTTCACCA

ACGTGTACGCCGACAGCTTCGTGATCAGGGGCAACGAGGTGAGCCA

GATCGCCCCCGGCCAGACCGGCAACATCGCCGACTACAACTACAAG

CTGCCCGACGACTTCACCGGCTGCGTGATCGCCTGGAACAGCAACA

AGCTGGACAGCAAGGTGGGCGGCAACTACAACTACCTGTACAGGCT

GTTCAGGAAGAGCAACCTGAAGCCCTTCGAGAGGGACATCAGCACC

GAGATCTACCAGGCCGGCAACAAGCCCTGCAACGGCGTGGCCGGCT

TCAACTGCTACTTCCCCCTGAGGAGCTACGGCTTCAGGCCCACCTAC

GGCGTGGGCCACCAGCCCTACAGGGTGGTGGTGCTGAGCTTCGAG

CTGCTGCACGCCCCCGCCACCGTGTGCGGCCCCAAGAAGAGCACCA

ACCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGCCTGACC

GGCACCGGCGTGCTGACCGAGAGCAACAAGAAGTTCCTGCCCTTCC

AGCAGTTCGGCAGGGACATCGCCGACACCACCGACGCCGTGAGGG

ACCCCCAGACCCTGGAGATCCTGGACATCACCCCCTGCAGCTTCGG

CGGCGTGAGCGTGATCACCCCCGGCACCAACACCAGCAACCAGGTG

GCCGTGCTGTACCAGGGCGTGAACTGCACCGAGGTGCCCGTGGCC

ATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTGTACAGCACCG

GCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGATCGGCGCCG

AGTACGTGAACAACAGCTACGAGTGCGACATCCCCATCGGCGCCGG

CATCTGCGCCAGCTACCAGACCCAGACCAAGAGCCACCAGCAGGCC

CAGAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAGCCTGG

GCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCATCCC

CACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGAGC

ATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACA

GCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCAC

CCAGCTGAAGAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACAA

GAACACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACC

CCCCCCATCAAGTACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCC

CGACCCCAGCAAGCCCAGCAAGAGGAGCCCCATCGAGGACCTGCTG

TTCAACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACG

GCGACTGCCTGGGCGACATCGCCGCCAGGGACCTGATCTGCGCCC

AGAAGTTCAACGGCCTGACCGTGCTGCCCCCCCTGCTGACCGACGA

GATGATCGCCCAGTACACCAGCGCCCTGCTGGCCGGCACCATCACC

AGCGGCTGGACCTTCGGCGCCGGCCCCGCCCTGCAGATCCCCTTCC

CCATGCAGATGGCCTACAGGTTCAACGGCATCGGCGTGACCCAGAA

CGTGCTGTACGAGAACCAGAAGCTGATCGCCAACCAGTTCAACAGC

GCCATCGGCAAGATCCAGGACAGCCTGAGCAGCACCCCCAGCGCCC

TGGGCAAGCTGCAGGACGTGGTGAACCACAACGCCCAGGCCCTGAA

CACCCTGGTGAAGCAGCTGAGCAGCAAGTTCGGCGCCATCAGCAGC

GTGCTGAACGACATCCTGAGCAGGCTGGACCCCCCCGAGGCCGAG

GTGCAGATCGACAGGCTGATCACCGGCAGGCTGCAGAGCCTGCAGA

CCTACGTGACCCAGCAGCTGATCAGGGCCGCCGAGATCAGGGCCAG

CGCCAACCTGGCCGCCACCAAGATGAGCGAGTGCGTGCTGGGCCA

GAGCAAGAGGGTGGACTTCTGCGGCAAGGGCTACCACCTGATGAGC

TTCCCCCAGAGCGCCCCCCACGGCGTGGTGTTCCTGCACGTGACCT

ACGTGCCCGCCCAGGAGAAGAACTTCACCACCGCCCCCGCCATCTG

CCACGACGGCAAGGCCCACTTCCCCAGGGAGGGCGTGTTCGTGAG

CAACGGCACCCACTGGTTCGTGACCCAGAGGAACTTCTACGAGCCC

CAGATCATCACCACCGACAACACCTTCGTGAGCGGCAACTGCGACG

TGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCTGCAGCC

CGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAAGAAC

CACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCAACG

CCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAGGT

GGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGGC

AAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGCTT

CATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTGCT

GCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGCGG

CAGCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGAAG

GGCGTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTCTC

TGTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTATGAAG

GCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGAC

ACATTATGTATAGGTTATCATGCGAACAACTCAACAGACACTGTAGAC

ACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTA

GAAGACAAGCATAACGGGAAACTATGCAAACTAAGAGGGGTAGCCC

CATTGCATTTGGGTAAATGTAACATTGCTGGCTGGATCCTGGGAAAT

CCAGAGTGTGAATCACTCTCCACAGCAAGCTCATGGTCCTACATTGT

GGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAGGAGATTTCA

TCGATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTCATCATTT

GAAAGGTTTGAGATATTCCCCAAGACAAGTTCATGGCCCAATCATGA

CTCGAACAAAGGTGTAACGACAGCATGTCCTCATGCTGGAGCAAAAA

GCTTCTACAAAAATTTAATATGGCTAGTTAAAAAAGGAAATTCATACC

CAAAGCTCAGCAAATCCTACATTAATGATAAAGGGAAAGAAGTCCTC

GTGCTATGGGGCATTCACCATCCACCTACTAGTGCTGACCAACAAAG

TCTCTATCAGAATGCAGATGCATATGTTTTTGTGGGGACATCAAGATA

CAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGGCCCAAAGTGAGG

GATCAAGAAGGGAGAATGAACTATTACTGGACACTAGTAGAGCCGGG

AGACAAAATAACATTCGAAGCAACTGGAAATCTAGTGGTACCGAGAT

ATGCATTCGCAATGGAAAGAAATGCTGGATCTGGTATTATCATTTCAG

ATACACCAGTCCACGATTGCAATACAACTTGTCAGACACCCAAGGGT

GCTATAAACACCAGCCTCCCATTTCAGAACATACATCCGATCACAATT

GGAAAATGTCCAAAATATGTAAAAAGCACAAAATTGAGACTGGCCAC

AGGATTGAGGAATGTCCCGTCCATTCAATCTAGAGGCCTATTTGGGG

CCATTGCCGGTTTCATTGAAGGGGGGTGGACAGGGATGGTAGATGG

ATGGTACGGTTATCACCATCAAAATGAGCAGGGGTCAGGATATGCAG

CCGACCTGAAGAGCACACAGAATGCCATTGACGAGATTACTAACAAA

GTAAACTCTGTTATTGAAAAAATGAATACACAGTTCACAGCAGTAGGT

AAAGAGTTCAACCACCTGGAAAAAAGAATAGAGAATTTAAATAAAAAA

GTTGATGATGGTTTCCTGGACATTTGGACTTACAATGCCGAACTGTTG

GTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAAGGTG

AAGAACTTATATGAAAAGGTAAGAAGCCAGTTAAAAAACAATGCCAAG

GAAATTGGAAACGGCTGCTTTGAATTTTACCACAAATGTGATAACACG

TGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACTCA

GAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGGTAAAGCTGGA

ATCAACAAGGATTTACCAGATTTTGGCGATCTATTCAACCGTCGCCAG

TTCATTGGTACTGGTAGTCTCCCTGGGGGCAATCAGTTTCTGGATGT

GCTCTAATGGGTCTCTACAGTGTAGAATATGTATTTAAGGAAGCGGA

GCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAAAA

CCCCGGGCCTAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGAC

TATCATTGCTTTGAGCTACATTCTATGTCTGGTTTTCGCTCAAAAACTT

CCTGGAAATGACAATAGCACTGCAACGCTGTGCCTTGGGCACCATGC

AGTACCAAACGGAACGATAGTGAAAACAATCACGAATGACCAAATTG

AAGTTACTAATGCTACTGAGCTGGTTCAGAATTCCTCAATAGGTGAAA

TATGCGACAGTCCTCATCAGATCCTTGATGGAGAAAACTGCACACTA

ATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA

GAAATGGGACCTTTTTGTTGAACGAAGCAAAGCCTACAGCAACTGTT

ACCCTTATGATGTGCCGGATTATGCCTCCCTTAGGTCACTAGTTGCC

TCATCCGGCACACTGGAGTTTAACAATGAAAGCTTCAATTGGGCTGG

AGTCACTCAAAACGGAACAAGTTCTGCTTGCATAAGGGGATCTAATA

GTAGTTTCTTTAGTAGATTAAATTGGTTGACCCACTTAAACTTCAAGTA

CCCAGCATTGAACGTGACTATGCCAAACAATGAACAATTTGACAAATT

GTACATTTGGGGGGTTCACCACCCGGGTACGGACAAGGACCAAATC

TTCCTGTATGCTCAATCATCAGGAAGAATCACAGTATCTACCAAAAGA

AGCCAACAAGCTGTAATCCCGAATATCGGATCTAGACCCAGAATAAG

GAATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAACCGG

GAGACATACTTTTGATTAACAGCACAGGGAATCTAATTGCTCCTAGG

GGTTACTTCAAAATACGAAGTGGGAAAAGCTCAATAATGAGATCAGAT

GCACCCATTGGCAAATGCAAGTCTGAATGCATCACTCCAAATGGAAG

CATTCCCAATGACAAACCATTCCAAAATGTAAACAGGATCACATACGG

GGCCTGTCCCAGATATGTTAAGCAAAGCACTCTGAAATTGGCAACAG

GAATGCGAAATGTACCAGAGAAACAAACTAGAGGCATATTTGGCGCA

ATAGCGGGTTTCATAGAAAATGGTTGGGAGGGAATGGTGGATGGTTG

GTACGGTTTCAGGCATCAAAATTCTGAGGGAAGAGGACAAGCAGCA

GATCTCAAAAGCACTCAAGCAGCAATCGATCAAATCAATGGGAAGCT

GAATCGATTGATCGGGAAAACCAACGAGAAATTCCATCAGATTGAGA

AAGAATTCTCAGAAGTAGAAGGGAGAATTCAGGACCTTGAGAAATAT

GTTGAGGACACAAAAATAGATCTCTGGTCATACAACGCAGAGCTTCT

TGTTGCCCTGGAAAACCAACATACAATTGATCTAACTGACTCAGAAAT

GAACAAACTGTTTGAAAAAACAAAGAAGCAACTGAGGGAAAATGCTG

AGGATATGGGCAATGGTTGTTTCAAAATATACCACAAATGTGACAATG

CCTGCATAGGATCAATCAGAAATGGAACTTATGACCACGATGTATACA

GGGATGAAGCATTAAACAACCGGTTCCAGATCAAGGGAGTTGAGCTG

AAGTCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCA

TGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCATCATGTGGGCCTGC

CAAAAGGGCAACATTAGGTGCAACATTTGCATTTGAGTGCATTAATTA

AAAACACCCTTGTTTCTACTGGAAGCGGAGCCACGAACTTCTCTCTG

TTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTATTTTCTA

ATATCCACAAAATGAAGGCAATAATTGTACTACTCATGGTAGTAACAT

CCAATGCAGATCGAATCTGCACTGGGATAACATCGTCAAACTCACCA

CATGTCGTCAAAACTGCTACTCAAGGGGAGGTCAACGTGACCGGTGT

AATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTCAA

AGGAACAGAAACCAGGGGGAAACTATGCCCAAAATGCCTCAACTGCA

CAGATCTGGATGTAGCCTTGGGCAGACCAAAATGCACAGGGAAAATA

CCCTCTGCAAGGGTTTCAATACTCCATGAAGTCAGACCTGTTACATCT

GGGTGCTTTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCC

TAACCTTCTCCGAGGATACGAACATGTCAGGTTATCAACTCACAACGT

TATCAATGCAAAAGATGCACCAGGAAGACCCTACGAAATTGGAACCT

CAGGGTCTTGCCCTAACATTACCAATGGAAACGGATTCTTCGCAACA

ATGGCTTGGGCCGTCCCAAAAAACAAAACAGCAACAAATCCATTAAC

AATAGAAGTACCATACATTTGTACAGAAGGAGAAGACCAAATTACCGT

TTGGGGGTTCCACTCTGACAACGAGACCCAAATGGCAAAGCTCTATG

GGGACTCAAAGCCCCAGAAGTTCACCTCATCTGCCAACGGAGTGAC

CACACATTACGTTTCACAGATTGGTGGCTTCCCAAATCAAACAGAAGA

CGGAGGACTACCACAAAGTGGCAGAATTGTTGTTGATTACATGGTGC

AGAAATCTGGAAAAACAGGAACAATTACCTATCAAAGAGGTATTTTAT

TGCCTCAAAAAGTGTGGTGCGCAAGTGGCAGGAGCAAGGTAATAAAA

GGATCCTTGCCCTTAATTGGAGAAGCAGATTGCCTCCATGAAAAATA

CGGTGGATTAAACAAAAGCAAGCCTTACTACACAGGGGAACATGCAA

AGGCCATAGGAAATTGCCCAATATGGGTGAAAACACCCTTGAAGCTG

GCCAATGGAACCAAATATAGACCCCCTGCAAAACTATTAAAGGAAAG

AGGTTTCTTCGGAGCCATTGCTGGTTTCTTAGAGGGAGGATGGGAAG

GAATGATTGCAGGTTGGCACGGATACACATCCCATGGGGCACATGG

AGTAGCGGTGGCAGCTGACCTTAAGAGCACTCAAGAGGCCATAAAC

AAGATAACAAAAAATCTCAACTCTTTGAGTGAGCTGGAAGTAAAGAAT

CTTCAAAGACTAAGCGGTGCCATGGATGAACTCCACAACGAAATACT

AGAACTAGATGAGAAAGTGGATGATCTCAGAGCTGACACAATAAGCT

CACAAATAGAACTCGCAGTCCTGCTTTCCAATGAAGGAATAATAAACA

GTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAAGAAAATG

CTGGGCCCCTCTGCTGTAGAGATAGGGAATGGATGCTTTGAAACCAA

ACACAAGTGCAACCAGACCTGCCTCGACAGAATAGCTGCTGGTACCT

TTGATGCAGGAGAATTTTCTCTCCCCACCTTTGATTCACTGAATATTA

CTGCTGCATCTTTAAATGACGACGGATTGGACAATCATACTATACTGC

TTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACACTGATGATAG

CTATCTTTGTTGTTTATATGGTCTCCAGAGACAATGTTTCTTGCTCCAT

TTGTCTATAAGGAAAGTTAAGCCCTGTATTTTCCTTTATTGTAGTGCTT

GTTTGCTTGTTGTCATTACAAAGAAACGTTATTGAAAAATTGATGACT

CGAGCTGGTACTGCATGCACGCAATGCTAGCTGCCCCTTTCCCGTCC

TGGGTACCCCGAGTCTCCCCCGACCTCGGGTCCCAGGTATGCTCCC

ACCTCCACCTGCCCCACTCACCACCTCTGCTAGTTCCAGACACCTCC

CAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCC

CCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAA

CTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACAC

CCTGGAGCTAGCAAAAAAAA

Pan-CoV-Flu
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATAT
404

Vaccine #16
ATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTG

CoV-Omicron
ACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTC

BA.2 Spike(2
CCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAG

proline
TATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATG

substitution)-
CCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTG

Influenza (HA)
GCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTA

CATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCA

GTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAA

GTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAAT

CAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCA

AATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT

GGTTTAGTGAACCGTCAGATCGAGAATAAACTAGTATTCTTCTGGTCC

CCACAGACTCAGAGAGAACCCGCCACCATGTTCGTGTTCCTGGTGCT

GCTGCCCCTGGTGAGCAGCCAGTGCGTGAACCTGATCACCAGGACC

CAGAGCTACACCAACAGCTTCACCAGGGGCGTGTACTACCCCGACA

AGGTGTTCAGGAGCAGCGTGCTGCACAGCACCCAGGACCTGTTCCT

GCCCTTCTTCAGCAACGTGACCTGGTTCCACGCCATCCACGTGAGC

GGCACCAACGGCACCAAGAGGTTCGACAACCCCGTGCTGCCCTTCA

ACGACGGCGTGTACTTCGCCAGCACCGAGAAGAGCAACATCATCAG

GGGCTGGATCTTCGGCACCACCCTGGACAGCAAGACCCAGAGCCTG

CTGATCGTGAACAACGCCACCAACGTGGTGATCAAGGTGTGCGAGTT

CCAGTTCTGCAACGACCCCTTCCTGGACGTGTACTACCACAAGAACA

ACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTACAGCAGCGCCAA

CAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCTGATGGACCTG

GAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGTTCGTGTTCA

AGAACATCGACGGCTACTTCAAGATCTACAGCAAGCACACCCCCATC

AACCTGGGCAGGGACCTGCCCCAGGGCTTCAGCGCCCTGGAGCCC

CTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTTCCAGACCCT

GCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGACAGCAGCAG

CGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGCTACCTGCA

GCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCACCATCACC

GACGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGACCAAGTGC

ACCCTGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAGACCAGCA

ACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTTCCCCAACAT

CACCAACCTGTGCCCCTTCGACGAGGTGTTCAACGCCACCAGGTTC

GCCAGCGTGTACGCCTGGAACAGGAAGAGGATCAGCAACTGCGTGG

CCGACTACAGCGTGCTGTACAACTTCGCCCCCTTCTTCGCCTTCAAG

TGCTACGGCGTGAGCCCCACCAAGCTGAACGACCTGTGCTTCACCA

ACGTGTACGCCGACAGCTTCGTGATCAGGGGCAACGAGGTGAGCCA

GATCGCCCCCGGCCAGACCGGCAACATCGCCGACTACAACTACAAG

CTGCCCGACGACTTCACCGGCTGCGTGATCGCCTGGAACAGCAACA

AGCTGGACAGCAAGGTGGGCGGCAACTACAACTACCTGTACAGGCT

GTTCAGGAAGAGCAACCTGAAGCCCTTCGAGAGGGACATCAGCACC

GAGATCTACCAGGCCGGCAACAAGCCCTGCAACGGCGTGGCCGGCT

TCAACTGCTACTTCCCCCTGAGGAGCTACGGCTTCAGGCCCACCTAC

GGCGTGGGCCACCAGCCCTACAGGGTGGTGGTGCTGAGCTTCGAG

CTGCTGCACGCCCCCGCCACCGTGTGCGGCCCCAAGAAGAGCACCA

ACCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACGGCCTGACC

GGCACCGGCGTGCTGACCGAGAGCAACAAGAAGTTCCTGCCCTTCC

AGCAGTTCGGCAGGGACATCGCCGACACCACCGACGCCGTGAGGG

ACCCCCAGACCCTGGAGATCCTGGACATCACCCCCTGCAGCTTCGG

CGGCGTGAGCGTGATCACCCCCGGCACCAACACCAGCAACCAGGTG

GCCGTGCTGTACCAGGGCGTGAACTGCACCGAGGTGCCCGTGGCC

ATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTGTACAGCACCG

GCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGATCGGCGCCG

AGTACGTGAACAACAGCTACGAGTGCGACATCCCCATCGGCGCCGG

CATCTGCGCCAGCTACCAGACCCAGACCAAGAGCCACCAGCAGGCC

CAGAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGAGCCTGG

GCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGCCATCCC

CACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCGTGAGC

ATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGGCGACA

GCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTCTGCAC

CCAGCTGAAGAGGGCCCTGACCGGCATCGCCGTGGAGCAGGACAA

GAACACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACAAGACC

CCCCCCATCAAGTACTTCGGCGGCTTCAACTTCAGCCAGATCCTGCC

CGACCCCAGCAAGCCCAGCAAGAGGAGCTTCATCGAGGACCTGCTG

TTCAACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGCAGTACG

GCGACTGCCTGGGCGACATCGCCGCCAGGGACCTGATCTGCGCCC

AGAAGTTCAACGGCCTGACCGTGCTGCCCCCCCTGCTGACCGACGA

GATGATCGCCCAGTACACCAGCGCCCTGCTGGCCGGCACCATCACC

AGCGGCTGGACCTTCGGCGCCGGCGCCGCCCTGCAGATCCCCTTC

GCCATGCAGATGGCCTACAGGTTCAACGGCATCGGCGTGACCCAGA

ACGTGCTGTACGAGAACCAGAAGCTGATCGCCAACCAGTTCAACAGC

GCCATCGGCAAGATCCAGGACAGCCTGAGCAGCACCGCCAGCGCC

CTGGGCAAGCTGCAGGACGTGGTGAACCACAACGCCCAGGCCCTGA

ACACCCTGGTGAAGCAGCTGAGCAGCAAGTTCGGCGCCATCAGCAG

CGTGCTGAACGACATCCTGAGCAGGCTGGACCCCCCCGAGGCCGA

GGTGCAGATCGACAGGCTGATCACCGGCAGGCTGCAGAGCCTGCAG

ACCTACGTGACCCAGCAGCTGATCAGGGCCGCCGAGATCAGGGCCA

GCGCCAACCTGGCCGCCACCAAGATGAGCGAGTGCGTGCTGGGCC

AGAGCAAGAGGGTGGACTTCTGCGGCAAGGGCTACCACCTGATGAG

CTTCCCCCAGAGCGCCCCCCACGGCGTGGTGTTCCTGCACGTGACC

TACGTGCCCGCCCAGGAGAAGAACTTCACCACCGCCCCCGCCATCT

GCCACGACGGCAAGGCCCACTTCCCCAGGGAGGGCGTGTTCGTGA

GCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTTCTACGAGCC

CCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAACTGCGAC

GTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCTGCAGC

CCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAAGAA

CCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATCAAC

GCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACGAGG

TGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCTGGG

CAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTGGGC

TTCATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCTGTG

CTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGCTGC

GGCAGCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTGCTGA

AGGGCGTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACTTCTC

TCTGTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTATGA

AGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAG

ACACATTATGTATAGGTTATCATGCGAACAACTCAACAGACACTGTAG

ACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTC

TAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGAGGGGTAGCC

CCATTGCATTTGGGTAAATGTAACATTGCTGGCTGGATCCTGGGAAA

TCCAGAGTGTGAATCACTCTCCACAGCAAGCTCATGGTCCTACATTG

TGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAGGAGATTTC

ATCGATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTCATCATTT

GAAAGGTTTGAGATATTCCCCAAGACAAGTTCATGGCCCAATCATGA

CTCGAACAAAGGTGTAACGACAGCATGTCCTCATGCTGGAGCAAAAA

GCTTCTACAAAAATTTAATATGGCTAGTTAAAAAAGGAAATTCATACC

CAAAGCTCAGCAAATCCTACATTAATGATAAAGGGAAAGAAGTCCTC

GTGCTATGGGGCATTCACCATCCACCTACTAGTGCTGACCAACAAAG

TCTCTATCAGAATGCAGATGCATATGTTTTTGTGGGGACATCAAGATA

CAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGGCCCAAAGTGAGG

GATCAAGAAGGGAGAATGAACTATTACTGGACACTAGTAGAGCCGGG

AGACAAAATAACATTCGAAGCAACTGGAAATCTAGTGGTACCGAGAT

ATGCATTCGCAATGGAAAGAAATGCTGGATCTGGTATTATCATTTCAG

ATACACCAGTCCACGATTGCAATACAACTTGTCAGACACCCAAGGGT

GCTATAAACACCAGCCTCCCATTTCAGAACATACATCCGATCACAATT

GGAAAATGTCCAAAATATGTAAAAAGCAGAAAATTGAGACTGGCCAC

AGGATTGAGGAATGTCCCGTCCATTCAATCTAGAGGCCTATTTGGGG

CCATTGCCGGTTTCATTGAAGGGGGGTGGACAGGGATGGTAGATGG

ATGGTACGGTTATCACCATCAAAATGAGCAGGGGTCAGGATATGCAG

CCGACCTGAAGAGCACACAGAATGCCATTGACGAGATTACTAACAAA

GTAAACTCTGTTATTGAAAAAATGAATACACAGTTCACAGCAGTAGGT

AAAGAGTTCAACCACCTGGAAAAAAGAATAGAGAATTTAAATAAAAAA

GTTGATGATGGTTTCCTGGACATTTGGACTTACAATGCCGAACTGTTG

GTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAAGGTG

AAGAACTTATATGAAAAGGTAAGAAGCCAGTTAAAAAACAATGCCAAG

GAAATTGGAAACGGCTGCTTTGAATTTTACCACAAATGTGATAACACG

TGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACTCA

GAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGGTAAAGCTGGA

ATCAACAAGGATTTACCAGATTTTGGCGATCTATTCAACCGTCGCCAG

TTCATTGGTACTGGTAGTCTCCCTGGGGGCAATCAGTTTCTGGATGT

GCTCTAATGGGTCTCTACAGTGTAGAATATGTATTTAAGGAAGCGGA

GCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAAAA

CCCCGGGCCTAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGAC

TATCATTGCTTTGAGCTACATTCTATGTCTGGTTTTCGCTCAAAAACTT

CCTGGAAATGACAATAGCACTGCAACGCTGTGCCTTGGGCACCATGC

AGTACCAAACGGAACGATAGTGAAAACAATCACGAATGACCAAATTG

AAGTTACTAATGCTACTGAGCTGGTTCAGAATTCCTCAATAGGTGAAA

TATGCGACAGTCCTCATCAGATCCTTGATGGAGAAAACTGCACACTA

ATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA

GAAATGGGACCTTTTTGTTGAACGAAGCAAAGCCTACAGCAACTGTT

ACCCTTATGATGTGCCGGATTATGCCTCCCTTAGGTCACTAGTTGCC

TCATCCGGCACACTGGAGTTTAACAATGAAAGCTTCAATTGGGCTGG

AGTCACTCAAAACGGAACAAGTTCTGCTTGCATAAGGGGATCTAATA

GTAGTTTCTTTAGTAGATTAAATTGGTTGACCCACTTAAACTTCAAGTA

CCCAGCATTGAACGTGACTATGCCAAACAATGAACAATTTGACAAATT

GTACATTTGGGGGGTTCACCACCCGGGTACGGACAAGGACCAAATC

TTCCTGTATGCTCAATCATCAGGAAGAATCACAGTATCTACCAAAAGA

AGCCAACAAGCTGTAATCCCGAATATCGGATCTAGACCCAGAATAAG

GAATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAACCGG

GAGACATACTTTGATTAACAGCACAGGGAATCTAATTGCTCCTAGG

GGTTACTTCAAAATACGAAGTGGGAAAAGCTCAATAATGAGATCAGAT

GCACCCATTGGCAAATGCAAGTCTGAATGCATCACTCCAAATGGAAG

CATTCCCAATGACAAACCATTCCAAAATGTAAACAGGATCACATACGG

GGCCTGTCCCAGATATGTTAAGCAAAGCACTCTGAAATTGGCAACAG

GAATGCGAAATGTACCAGAGAAACAAACTAGAGGCATATTTGGCGCA

ATAGCGGGTTTCATAGAAAATGGTTGGGAGGGAATGGTGGATGGTTG

GTACGGTTTCAGGCATCAAAATTCTGAGGGAAGAGGACAAGCAGCA

GATCTCAAAAGCACTCAAGCAGCAATCGATCAAATCAATGGGAAGCT

GAATCGATTGATCGGGAAAACCAACGAGAAATTCCATCAGATTGAGA

AAGAATTCTCAGAAGTAGAAGGGAGAATTCAGGACCTTGAGAAATAT

GTTGAGGACACAAAAATAGATCTCTGGTCATACAACGCAGAGCTTCT

TGTTGCCCTGGAAAACCAACATACAATTGATCTAACTGACTCAGAAAT

GAACAAACTGTTTGAAAAAACAAAGAAGCAACTGAGGGAAAATGCTG

AGGATATGGGCAATGGTTGTTTCAAAATATACCACAAATGTGACAATG

CCTGCATAGGATCAATCAGAAATGGAACTTATGACCACGATGTATACA

GGGATGAAGCATTAAACAACCGGTTCCAGATCAAGGGAGTTGAGCTG

AAGTCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCA

TGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCATCATGTGGGCCTGC

CAAAAGGGCAACATTAGGTGCAACATTTGCATTTGAGTGCATTAATTA

AAAACACCCTTGTTTCTACTGGAAGCGGAGCCACGAACTTCTCTCTG

TTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTATTTTCTA

ATATCCACAAAATGAAGGCAATAATTGTACTACTCATGGTAGTAACAT

CCAATGCAGATCGAATCTGCACTGGGATAACATCGTCAAACTCACCA

CATGTCGTCAAAACTGCTACTCAAGGGGAGGTCAACGTGACCGGTGT

AATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTCAA

AGGAACAGAAACCAGGGGGAAACTATGCCCAAAATGCCTCAACTGCA

CAGATCTGGATGTAGCCTTGGGCAGACCAAAATGCACAGGGAAAATA

CCCTCTGCAAGGGTTTCAATACTCCATGAAGTCAGACCTGTTACATCT

GGGTGCTTTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCC

TAACCTTCTCCGAGGATACGAACATGTCAGGTTATCAACTCACAACGT

TATCAATGCAAAAGATGCACCAGGAAGACCCTACGAAATTGGAACCT

CAGGGTCTTGCCCTAACATTACCAATGGAAACGGATTCTTCGCAACA

ATGGCTTGGGCCGTCCCAAAAAACAAAACAGCAACAAATCCATTAAC

AATAGAAGTACCATACATTTGTACAGAAGGAGAAGACCAAATTACCGT

TTGGGGGTTCCACTCTGACAACGAGACCCAAATGGCAAAGCTCTATG

GGGACTCAAAGCCCCAGAAGTTCACCTCATCTGCCAACGGAGTGAC

CACACATTACGTTTCACAGATTGGTGGCTTCCCAAATCAAACAGAAGA

CGGAGGACTACCACAAAGTGGCAGAATTGTTGTTGATTACATGGTGC

AGAAATCTGGAAAAACAGGAACAATTACCTATCAAAGAGGTATTTTAT

TGCCTCAAAAAGTGTGGTGCGCAAGTGGCAGGAGCAAGGTAATAAAA

GGATCCTTGCCCTTAATTGGAGAAGCAGATTGCCTCCATGAAAAATA

CGGTGGATTAAACAAAAGCAAGCCTTACTACACAGGGGAACATGCAA

AGGCCATAGGAAATTGCCCAATATGGGTGAAAACACCCTTGAAGCTG

GCCAATGGAACCAAATATAGACCCCCTGCAAAACTATTAAAGGAAAG

AGGTTTCTTCGGAGCCATTGCTGGTTTCTTAGAGGGAGGATGGGAAG

GAATGATTGCAGGTTGGCACGGATACACATCCCATGGGGCACATGG

AGTAGCGGTGGCAGCTGACCTTAAGAGCACTCAAGAGGCCATAAAC

AAGATAACAAAAAATCTCAACTCTTTGAGTGAGCTGGAAGTAAAGAAT

CTTCAAAGACTAAGCGGTGCCATGGATGAACTCCACAACGAAATACT

AGAACTAGATGAGAAAGTGGATGATCTCAGAGCTGACACAATAAGCT

CACAAATAGAACTCGCAGTCCTGCTTTCCAATGAAGGAATAATAAACA

GTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAAGAAAATG

CTGGGCCCCTCTGCTGTAGAGATAGGGAATGGATGCTTTGAAACCAA

ACACAAGTGCAACCAGACCTGCCTCGACAGAATAGCTGCTGGTACCT

TTGATGCAGGAGAATTTTCTCTCCCCACCTTTGATTCACTGAATATTA

CTGCTGCATCTTTAAATGACGACGGATTGGACAATCATACTATACTGC

TTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACACTGATGATAG

CTATCTTTGTTGTTTATATGGTCTCCAGAGACAATGTTTCTTGCTCCAT

TTGTCTATAAGGAAAGTTAAGCCCTGTATTTTCCTTTATTGTAGTGCTT

GTTTGCTTGTTGTCATTACAAAGAAACGTTATTGAAAAATTGATGACT

CGAGCTGGTACTGCATGCACGCAATGCTAGCTGCCCCTTTCCCGTCC

TGGGTACCCCGAGTCTCCCCCGACCTCGGGTCCCAGGTATGCTCCC

ACCTCCACCTGCCCCACTCACCACCTCTGCTAGTTCCAGACACCTCC

CAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCC

CCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAA

CTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACAC

CCTGGAGCTAGCAAAAAAAA

Pan-CoV-Flu
CTCGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGT
405

Vaccine #17
CATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGG

CoV- Omicron
TAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGAC

BA.2
GTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCA

Spike(HexaPro)-
TTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAG

Influenza (HA)
TACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATG

ACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGG

GACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACC

ATGGTCGAGGTGAGCCCCACGTTCTGCTTCACTCTCCCCATCTCCCC

CCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTTTGT

GCAGCGATGGGGGCGGGGGGGGGGGGGGGGCGCGCGCCAGGCG

GGGCGGGGCGGGGCGAGGGGCGGGGCGGGGCGAGGCGGAGAGG

TGCGGCGGCAGCCAATCAGAGCGGCGCGCTCCGAAAGTTTCCTTTT

ATGGCGAGGCGGCGGCGGCGGCGGCCCTATAAAAAGCGAAGCGCG

CGGCGGGCGGGAGTCGCTGCGCGCTGCCTTCGCCCCGTGCCCCGC

TCCGCCGCCGCCTCGCGCCGCCCGCCCCGGCTCTGACTGACCGCG

TTACTCCCACAGGTGAGCGGGCGGGACGGCCCTTCTCCTCCGGGCT

GTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGC

GTGAAAGCCTTGAGGGGCTCCGGGAGGGCCCTTTGTGCGGGGGGA

GCGGCTCGGGGGGTGCGTGCGTGTGTGTGTGCGTGGGGAGCGCCG

CGTGCGGCTCCGCGCTGCCCGGCGGCTGTGAGCGCTGCGGGCGCG

GCGCGGGGCTTTGTGCGCTCCGCAGTGTGCGCGAGGGGAGCGCGG

CCGGGGGCGGTGCCCCGCGGTGCGGGGGGGGCTGCGAGGGGAAC

AAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGGGTGAGCAGGGGGT

GTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTGCACCCCCCTCCC

CGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGGGCTCCGTACG

GGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGGTGGCGGCA

GGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCCGGGGAG

GGCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGCGGCTG

TCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCGTGC

GAGAGGGCGCAGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCGA

AATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGA

AGCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTC

GTGCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGG

CTGTCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGG

CGGGGTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGC

TAACCATGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGT

GCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAGAATTGGAGAATAA

ACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGAACCCGCCACCA

TGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGT

GAACCTGATCACCAGGACCCAGAGCTACACCAACAGCTTCACCAGG

GGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCTGCACA

GCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTGGTTC

CACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGACA

ACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGAC

AGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGG

TGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGAC

GTGTACTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCA

GGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCA

GCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAAC

CTGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTA

CAGCAAGCACACCCCCATCAACCTGGGCAGGGACCTGCCCCAGGGC

TTCAGCGCCCTGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACA

TCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGAC

CCCCGGCGACAGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTA

CTACGTGGGCTACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAAC

GAGAACGGCACCATCACCGACGCCGTGGACTGCGCCCTGGACCCC

CTGAGCGAGACCAAGTGCACCCTGAAGAGCTTCACCGTGGAGAAGG

GCATCTACCAGACCAGCAACTTCAGGGTGCAGCCCACCGAGAGCAT

CGTGAGGTTCCCCAACATCACCAACCTGTGCCCCTTCGACGAGGTGT

TCAACGCCACCAGGTTCGCCAGCGTGTACGCCTGGAACAGGAAGAG

GATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTACAACTTCGCC

CCCTTCTTCGCCTTCAAGTGCTACGGCGTGAGCCCCACCAAGCTGAA

CGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTCGTGATCAGG

GGCAACGAGGTGAGCCAGATCGCCCCCGGCCAGACCGGCAACATC

GCCGACTACAACTACAAGCTGCCCGACGACTTCACCGGCTGCGTGA

TCGCCTGGAACAGCAACAAGCTGGACAGCAAGGTGGGCGGCAACTA

CAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGAAGCCCTTCG

AGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAACAAGCCCTG

CAACGGCGTGGCCGGCTTCAACTGCTACTTCCCCCTGAGGAGCTAC

GGCTTCAGGCCCACCTACGGCGTGGGCCACCAGCCCTACAGGGTG

GTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGTGCG

GCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACTT

CAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAAC

AAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACA

CCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACAT

CACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACC

AACACCAGCAACCAGGTGGCCGTGCTGTACCAGGGCGTGAACTGCA

CCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTG

GAGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGG

CTGCCTGATCGGCGCCGAGTACGTGAACAACAGCTACGAGTGCGAC

ATCCCCATCGGCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCA

AGAGCCACCAGCAGGCCCAGAGCGTGGCCAGCCAGAGCATCATCG

CCTACACCATGAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAA

CAACAGCATCGCCATCCCCACCAACTTCACCATCAGCGTGACCACCG

AGATCCTGCCCGTGAGCATGACCAAGACCAGCGTGGACTGCACCAT

GTACATCTGCGGCGACAGCACCGAGTGCAGCAACCTGCTGCTGCAG

TACGGCAGCTTCTGCACCCAGCTGAAGAGGGCCCTGACCGGCATCG

CCGTGGAGCAGGACAAGAACACCCAGGAGGTGTTCGCCCAGGTGAA

GCAGATCTACAAGACCCCCCCCATCAAGTACTTCGGCGGCTTCAACT

TCAGCCAGATCCTGCCCGACCCCAGCAAGCCCAGCAAGAGGAGCCC

CATCGAGGACCTGCTGTTCAACAAGGTGACCCTGGCCGACGCCGGC

TTCATCAAGCAGTACGGCGACTGCCTGGGCGACATCGCCGCCAGGG

ACCTGATCTGCGCCCAGAAGTTCAACGGCCTGACCGTGCTGCCCCC

CCTGCTGACCGACGAGATGATCGCCCAGTACACCAGCGCCCTGCTG

GCCGGCACCATCACCAGCGGCTGGACCTTCGGCGCCGGCCCCGCC

CTGCAGATCCCCTTCCCCATGCAGATGGCCTACAGGTTCAACGGCAT

CGGCGTGACCCAGAACGTGCTGTACGAGAACCAGAAGCTGATCGCC

AACCAGTTCAACAGCGCCATCGGCAAGATCCAGGACAGCCTGAGCA

GCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGTGGTGAACCACAA

CGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAGCAGCAAGTTC

GGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAGGCTGGACC

CCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCGGCAGGC

TGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGGGCCGC

CGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAGCGA

GTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGGG

CTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTG

TTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCA

CCGCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGA

GGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGG

AACTTCTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAG

CGGCAACTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTAC

GACCCCCTGCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACA

AGTACTTCAAGAACCACACCAGCCCCGACGTGGACCTGGGCGACAT

CAGCGGCATCAACGCCAGCGTGGTGAACATCCAGAAGGAGATCGAC

AGGCTGAACGAGGTGGCCAAGAACCTGAACGAGAGCCTGATCGACC

TGCAGGAGCTGGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGTA

CATCTGGCTGGGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTG

ACCATCATGCTGTGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGG

GCTGCTGCAGCTGCGGCAGCTGCTGCAAGTTCGACGAGGACGACAG

CGAGCCCGTGCTGAAGGGCGTGAAGCTGCACTACACCGGAAGCGG

AGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAAA

ACCCCGGGCCTATGAAGGCAATACTAGTAGTTCTGCTATATACATTTG

CAACCGCAAATGCAGACACATTATGTATAGGTTATCATGCGAACAACT

CAACAGACACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACA

CACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAA

CTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGCTGG

CTGGATCCTGGGAAATCCAGAGTGTGAATCACTCTCCACAGCAAGCT

CATGGTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTT

ACCCAGGAGATTTCATCGATTATGAGGAGCTAAGAGAGCAATTGAGC

TCAGTGTCATCATTTGAAAGGTTTGAGATATTCCCCAAGACAAGTTCA

TGGCCCAATCATGACTCGAACAAAGGTGTAACGACAGCATGTCCTCA

TGCTGGAGCAAAAAGCTTCTACAAAAATTTAATATGGCTAGTTAAAAA

AGGAAATTCATACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGG

GAAAGAAGTCCTCGTGCTATGGGGCATTCACCATCCACCTACTAGTG

CTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTTTTGTGG

GGACATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGG

CCCAAAGTGAGGGATCAAGAAGGGAGAATGAACTATTACTGGACACT

AGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAG

TGGTACCGAGATATGCATTCGCAATGGAAAGAAATGCTGGATCTGGT

ATTATCATTTCAGATACACCAGTCCACGATTGCAATACAACTTGTCAG

ACACCCAAGGGTGCTATAAACACCAGCCTCCCATTTCAGAACATACA

TCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGCACAAAATT

GAGACTGGCCACAGGATTGAGGAATGTCCCGTCCATTCAATCTAGAG

GCCTATTTGGGGCCATTGCCGGTTTCATTGAAGGGGGGTGGACAGG

GATGGTAGATGGATGGTACGGTTATCACCATCAAAATGAGCAGGGGT

CAGGATATGCAGCCGACCTGAAGAGCACACAGAATGCCATTGACGA

GATTACTAACAAAGTAAACTCTGTTATTGAAAAAATGAATACACAGTTC

ACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGAA

TTTAAATAAAAAAGTTGATGATGGTTTCCTGGACATTTGGACTTACAAT

GCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCA

CGATTCAAAGGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGTTAAA

AAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACCACAA

ATGTGATAACACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACT

ACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGAT

GGGGTAAAGCTGGAATCAACAAGGATTTACCAGATTTTGGCGATCTA

TTCAACCGTCGCCAGTTCATTGGTACTGGTAGTCTCCCTGGGGGCAA

TCAGTTTCTGGATGTGCTCTAATGGGTCTCTACAGTGTAGAATATGTA

TTTAAGGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGA

GATGTTGAAGAAAACCCCGGGCCTAGCAAAAGCAGGGGATAATTCTA

TTAACCATGAAGACTATCATTGCTTTGAGCTACATTCTATGTCTGGTTT

TCGCTCAAAAACTTCCTGGAAATGACAATAGCACTGCAACGCTGTGC

CTTGGGCACCATGCAGTACCAAACGGAACGATAGTGAAAACAATCAC

GAATGACCAAATTGAAGTTACTAATGCTACTGAGCTGGTTCAGAATTC

CTCAATAGGTGAAATATGCGACAGTCCTCATCAGATCCTTGATGGAG

AAAACTGCACACTAATAGATGCTCTATTGGGAGACCCTCAGTGTGAT

GGCTTTCAAAATAAGAAATGGGACCTTTTTGTTGAACGAAGCAAAGC

CTACAGCAACTGTTACCCTTATGATGTGCCGGATTATGCCTCCCTTAG

GTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGAAAGCT

TCAATTGGGCTGGAGTCACTCAAAACGGAACAAGTTCTGCTTGCATA

AGGGGATCTAATAGTAGTTTCTTTAGTAGATTAAATTGGTTGACCCAC

TTAAACTTCAAGTACCCAGCATTGAACGTGACTATGCCAAACAATGAA

CAATTTGACAAATTGTACATTTGGGGGGTTCACCACCCGGGTACGGA

CAAGGACCAAATCTTCCTGTATGCTCAATCATCAGGAAGAATCACAGT

ATCTACCAAAAGAAGCCAACAAGCTGTAATCCCGAATATCGGATCTA

GACCCAGAATAAGGAATATCCCTAGCAGAATAAGCATCTATTGGACA

ATAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAGGGAATCTA

ATTGCTCCTAGGGGTTACTTCAAAATACGAAGTGGGAAAAGCTCAAT

AATGAGATCAGATGCACCCATTGGCAAATGCAAGTCTGAATGCATCA

CTCCAAATGGAAGCATTCCCAATGACAAACCATTCCAAAATGTAAACA

GGATCACATACGGGGCCTGTCCCAGATATGTTAAGCAAAGCACTCTG

AAATTGGCAACAGGAATGCGAAATGTACCAGAGAAACAAACTAGAGG

CATATTTGGCGCAATAGCGGGTTTCATAGAAAATGGTTGGGAGGGAA

TGGTGGATGGTTGGTACGGTTTCAGGCATCAAAATTCTGAGGGAAGA

GGACAAGCAGCAGATCTCAAAAGCACTCAAGCAGCAATCGATCAAAT

CAATGGGAAGCTGAATCGATTGATCGGGAAAACCAACGAGAAATTCC

ATCAGATTGAGAAAGAATTCTCAGAAGTAGAAGGGAGAATTCAGGAC

CTTGAGAAATATGTTGAGGACACAAAAATAGATCTCTGGTCATACAAC

GCAGAGCTTCTTGTTGCCCTGGAAAACCAACATACAATTGATCTAACT

GACTCAGAAATGAACAAACTGTTTGAAAAAACAAAGAAGCAACTGAG

GGAAAATGCTGAGGATATGGGCAATGGTTGTTTCAAAATATACCACA

AATGTGACAATGCCTGCATAGGATCAATCAGAAATGGAACTTATGAC

CACGATGTATACAGGGATGAAGCATTAAACAACCGGTTCCAGATCAA

GGGAGTTGAGCTGAAGTCAGGGTACAAAGATTGGATCCTATGGATTT

CCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCAT

CATGTGGGCCTGCCAAAAGGGCAACATTAGGTGCAACATTTGCATTT

GAGTGCATTAATTAAAAACACCCTTGTTTCTACTGGAAGCGGAGCCA

CGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCC

GGGCCTATTTTCTAATATCCACAAAATGAAGGCAATAATTGTACTACT

CATGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACAT

CGTCAAACTCACCACATGTCGTCAAAACTGCTACTCAAGGGGAGGTC

AACGTGACCGGTGTAATACCACTGACAACAACACCCACCAAATCTCA

TTTTGCAAATCTCAAAGGAACAGAAACCAGGGGGAAACTATGCCCAA

AATGCCTCAACTGCACAGATCTGGATGTAGCCTTGGGCAGACCAAAA

TGCACAGGGAAAATACCCTCTGCAAGGGTTTCAATACTCCATGAAGT

CAGACCTGTTACATCTGGGTGCTTTCCTATAATGCACGATAGAACAAA

AATTAGACAGCTGCCTAACCTTCTCCGAGGATACGAACATGTCAGGT

TATCAACTCACAACGTTATCAATGCAAAAGATGCACCAGGAAGACCC

TACGAAATTGGAACCTCAGGGTCTTGCCCTAACATTACCAATGGAAA

CGGATTCTTCGCAACAATGGCTTGGGCCGTCCCAAAAAACAAAACAG

CAACAAATCCATTAACAATAGAAGTACCATACATTTGTACAGAAGGAG

AAGACCAAATTACCGTTTGGGGGTTCCACTCTGACAACGAGACCCAA

ATGGCAAAGCTCTATGGGGACTCAAAGCCCCAGAAGTTCACCTCATC

TGCCAACGGAGTGACCACACATTACGTTTCACAGATTGGTGGCTTCC

CAAATCAAACAGAAGACGGAGGACTACCACAAAGTGGCAGAATTGTT

GTTGATTACATGGTGCAGAAATCTGGAAAAACAGGAACAATTACCTAT

CAAAGAGGTATTTTATTGCCTCAAAAAGTGTGGTGCGCAAGTGGCAG

GAGCAAGGTAATAAAAGGATCCTTGCCCTTAATTGGAGAAGCAGATT

GCCTCCATGAAAAATACGGTGGATTAAACAAAAGCAAGCCTTACTAC

ACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGGTGAA

AACACCCTTGAAGCTGGCCAATGGAACCAAATATAGACCCCCTGCAA

AACTATTAAAGGAAAGAGGTTTCTTCGGAGCCATTGCTGGTTTCTTAG

AGGGAGGATGGGAAGGAATGATTGCAGGTTGGCACGGATACACATC

CCATGGGGCACATGGAGTAGCGGTGGCAGCTGACCTTAAGAGCACT

CAAGAGGCCATAAACAAGATAACAAAAAATCTCAACTCTTTGAGTGAG

CTGGAAGTAAAGAATCTTCAAAGACTAAGCGGTGCCATGGATGAACT

CCACAACGAAATACTAGAACTAGATGAGAAAGTGGATGATCTCAGAG

CTGACACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTTCCAATG

AAGGAATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGA

AAGCTGAAGAAAATGCTGGGCCCCTCTGCTGTAGAGATAGGGAATG

GATGCTTTGAAACCAAACACAAGTGCAACCAGACCTGCCTCGACAGA

ATAGCTGCTGGTACCTTTGATGCAGGAGAATTTTCTCTCCCCACCTTT

GATTCACTGAATATTACTGCTGCATCTTTAAATGACGACGGATTGGAC

AATCATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCT

GTAACACTGATGATAGCTATCTTTGTTGTTTATATGGTCTCCAGAGAC

AATGTTTCTTGCTCCATTTGTCTATAAGGAAAGTTAAGCCCTGTATTTT

CCTTTATTGTAGTGCTTGTTTGCTTGTTGTCATTACAAAGAAACGTTAT

TGAAAAATTGATGACTCGAGCTGGTACTGCATGCACGCAATGCTAGC

TGCCCCTTTCCCGTCCTGGGTACCCCGAGTCTCCCCCGACCTCGGG

TCCCAGGTATGCTCCCACCTCCACCTGCCCCACTCACCACCTCTGCT

AGTTCCAGACACCTCCCAAGCACGCAGCAATGCAGCTCAAAACGCTT

AGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCA

ATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATT

TCGTGCCAGCCACACCCTGGAGCTAGCAAAAAAAA

Pan-CoV-Flu
CTCGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGT
406

Vaccine #18
CATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGG

CoV-Omicron
TAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGAC

BA.2 Spike(2
GTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCA

proline
TTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAG

substitution)-
TACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATG

Influenza (HA)
ACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGG

GACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACC

ATGGTCGAGGTGAGCCCCACGTTCTGCTTCACTCTCCCCATCTCCCC

CCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTTTGT

GCAGCGATGGGGGCGGGGGGGGGGGGGGGGCGCGCGCCAGGCG

GGGCGGGGCGGGGCGAGGGGCGGGGCGGGGCGAGGCGGAGAGG

TGCGGCGGCAGCCAATCAGAGCGGCGCGCTCCGAAAGTTTCCTTTT

ATGGCGAGGCGGCGGCGGCGGCGGCCCTATAAAAAGCGAAGCGCG

CGGCGGGCGGGAGTCGCTGCGCGCTGCCTTCGCCCCGTGCCCCGC

TCCGCCGCCGCCTCGCGCCGCCCGCCCCGGCTCTGACTGACCGCG

TTACTCCCACAGGTGAGCGGGCGGGACGGCCCTTCTCCTCCGGGCT

GTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGC

GTGAAAGCCTTGAGGGGCTCCGGGAGGGCCCTTTGTGCGGGGGGA

GCGGCTCGGGGGGTGCGTGCGTGTGTGTGTGCGTGGGGAGCGCCG

CGTGCGGCTCCGCGCTGCCCGGCGGCTGTGAGCGCTGCGGGCGCG

GCGCGGGGCTTTGTGCGCTCCGCAGTGTGCGCGAGGGGAGCGCGG

CCGGGGGCGGTGCCCCGCGGTGCGGGGGGGGCTGCGAGGGGAAC

AAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGGGTGAGCAGGGGGT

GTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTGCACCCCCCTCCC

CGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGGGCTCCGTACG

GGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGGTGGCGGCA

GGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCCGGGGAG

GGCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGCGGCTG

TCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCGTGC

GAGAGGGCGCAGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCGA

AATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGA

AGCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTC

GTGCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGG

CTGTCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGG

CGGGGTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGC

TAACCATGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGT

GCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAGAATTGGAGAATAA

ACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGAACCCGCCACCA

TGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGT

GAACCTGATCACCAGGACCCAGAGCTACACCAACAGCTTCACCAGG

GGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCTGCACA

GCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTGGTTC

CACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGACA

ACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGAC

AGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGG

TGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGAC

GTGTACTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCA

GGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCA

GCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAAC

CTGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTA

CAGCAAGCACACCCCCATCAACCTGGGCAGGGACCTGCCCCAGGGC

TTCAGCGCCCTGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACA

TCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGAC

CCCCGGCGACAGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTA

CTACGTGGGCTACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAAC

GAGAACGGCACCATCACCGACGCCGTGGACTGCGCCCTGGACCCC

CTGAGCGAGACCAAGTGCACCCTGAAGAGCTTCACCGTGGAGAAGG

GCATCTACCAGACCAGCAACTTCAGGGTGCAGCCCACCGAGAGCAT

CGTGAGGTTCCCCAACATCACCAACCTGTGCCCCTTCGACGAGGTGT

TCAACGCCACCAGGTTCGCCAGCGTGTACGCCTGGAACAGGAAGAG

GATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTACAACTTCGCC

CCCTTCTTCGCCTTCAAGTGCTACGGCGTGAGCCCCACCAAGCTGAA

CGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTCGTGATCAGG

GGCAACGAGGTGAGCCAGATCGCCCCCGGCCAGACCGGCAACATC

GCCGACTACAACTACAAGCTGCCCGACGACTTCACCGGCTGCGTGA

TCGCCTGGAACAGCAACAAGCTGGACAGCAAGGTGGGCGGCAACTA

CAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGAAGCCCTTCG

AGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAACAAGCCCTG

CAACGGCGTGGCCGGCTTCAACTGCTACTTCCCCCTGAGGAGCTAC

GGCTTCAGGCCCACCTACGGCGTGGGCCACCAGCCCTACAGGGTG

GTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGTGCG

GCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACTT

CAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAAC

AAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACA

CCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACAT

CACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACC

AACACCAGCAACCAGGTGGCCGTGCTGTACCAGGGCGTGAACTGCA

CCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTG

GAGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGG

CTGCCTGATCGGCGCCGAGTACGTGAACAACAGCTACGAGTGCGAC

ATCCCCATCGGCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCA

AGAGCCACCAGCAGGCCCAGAGCGTGGCCAGCCAGAGCATCATCG

CCTACACCATGAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAA

CAACAGCATCGCCATCCCCACCAACTTCACCATCAGCGTGACCACCG

AGATCCTGCCCGTGAGCATGACCAAGACCAGCGTGGACTGCACCAT

GTACATCTGCGGCGACAGCACCGAGTGCAGCAACCTGCTGCTGCAG

TACGGCAGCTTCTGCACCCAGCTGAAGAGGGCCCTGACCGGCATCG

CCGTGGAGCAGGACAAGAACACCCAGGAGGTGTTCGCCCAGGTGAA

GCAGATCTACAAGACCCCCCCCATCAAGTACTTCGGCGGCTTCAACT

TCAGCCAGATCCTGCCCGACCCCAGCAAGCCCAGCAAGAGGAGCTT

CATCGAGGACCTGCTGTTCAACAAGGTGACCCTGGCCGACGCCGGC

TTCATCAAGCAGTACGGCGACTGCCTGGGCGACATCGCCGCCAGGG

ACCTGATCTGCGCCCAGAAGTTCAACGGCCTGACCGTGCTGCCCCC

CCTGCTGACCGACGAGATGATCGCCCAGTACACCAGCGCCCTGCTG

GCCGGCACCATCACCAGCGGCTGGACCTTCGGCGCCGGCGCCGCC

CTGCAGATCCCCTTCGCCATGCAGATGGCCTACAGGTTCAACGGCAT

CGGCGTGACCCAGAACGTGCTGTACGAGAACCAGAAGCTGATCGCC

AACCAGTTCAACAGCGCCATCGGCAAGATCCAGGACAGCCTGAGCA

GCACCGCCAGCGCCCTGGGCAAGCTGCAGGACGTGGTGAACCACA

ACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAGCAGCAAGTT

CGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAGGCTGGAC

CCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCGGCAGG

CTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGGGCCG

CCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAGCG

AGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGG

GCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGT

GTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACC

ACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGG

AGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAG

GAACTTCTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGA

GCGGCAACTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTA

CGACCCCCTGCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGAC

AAGTACTTCAAGAACCACACCAGCCCCGACGTGGACCTGGGCGACA

TCAGCGGCATCAACGCCAGCGTGGTGAACATCCAGAAGGAGATCGA

CAGGCTGAACGAGGTGGCCAAGAACCTGAACGAGAGCCTGATCGAC

CTGCAGGAGCTGGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGT

ACATCTGGCTGGGCTTCATCGCCGGCCTGATCGCCATCGTGATGGT

GACCATCATGCTGTGCTGCATGACCAGCTGCTGCAGCTGCCTGAAG

GGCTGCTGCAGCTGCGGCAGCTGCTGCAAGTTCGACGAGGACGACA

GCGAGCCCGTGCTGAAGGGCGTGAAGCTGCACTACACCGGAAGCG

GAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAA

AACCCCGGGCCTATGAAGGCAATACTAGTAGTTCTGCTATATACATTT

GCAACCGCAAATGCAGACACATTATGTATAGGTTATCATGCGAACAA

CTCAACAGACACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAA

CACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGC

AAACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGC

TGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCTCCACAGCAA

GCTCATGGTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACG

TGTTACCCAGGAGATTTCATCGATTATGAGGAGCTAAGAGAGCAATT

GAGCTCAGTGTCATCATTTGAAAGGTTTGAGATATTCCCCAAGACAA

GTTCATGGCCCAATCATGACTCGAACAAAGGTGTAACGACAGCATGT

CCTCATGCTGGAGCAAAAAGCTTCTACAAAAATTTAATATGGCTAGTT

AAAAAAGGAAATTCATACCCAAAGCTCAGCAAATCCTACATTAATGAT

AAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTCACCATCCACCTAC

TAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTTT

TGTGGGGACATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAA

TAAGGCCCAAAGTGAGGGATCAAGAAGGGAGAATGAACTATTACTGG

ACACTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAA

TCTAGTGGTACCGAGATATGCATTCGCAATGGAAAGAAATGCTGGAT

CTGGTATTATCATTTCAGATACACCAGTCCACGATTGCAATACAACTT

GTCAGACACCCAAGGGTGCTATAAACACCAGCCTCCCATTTCAGAAC

ATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGCACA

AAATTGAGACTGGCCACAGGATTGAGGAATGTCCCGTCCATTCAATC

TAGAGGCCTATTTGGGGCCATTGCCGGTTTCATTGAAGGGGGGTGG

ACAGGGATGGTAGATGGATGGTACGGTTATCACCATCAAAATGAGCA

GGGGTCAGGATATGCAGCCGACCTGAAGAGCACACAGAATGCCATT

GACGAGATTACTAACAAAGTAAACTCTGTTATTGAAAAAATGAATACA

CAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAAT

AGAGAATTTAAATAAAAAAGTTGATGATGGTTTCCTGGACATTTGGAC

TTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGA

CTACCACGATTCAAAGGTGAAGAACTTATATGAAAAGGTAAGAAGCC

AGTTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTT

ACCACAAATGTGATAACACGTGCATGGAAAGTGTCAAAAATGGGACT

TATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAA

ATAGATGGGGTAAAGCTGGAATCAACAAGGATTTACCAGATTTTGGC

GATCTATTCAACCGTCGCCAGTTCATTGGTACTGGTAGTCTCCCTGG

GGGCAATCAGTTTCTGGATGTGCTCTAATGGGTCTCTACAGTGTAGA

ATATGTATTTAAGGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAA

GCAGGAGATGTTGAAGAAAACCCCGGGCCTAGCAAAAGCAGGGGAT

AATTCTATTAACCATGAAGACTATCATTGCTTTGAGCTACATTCTATGT

CTGGTTTTCGCTCAAAAACTTCCTGGAAATGACAATAGCACTGCAAC

GCTGTGCCTTGGGCACCATGCAGTACCAAACGGAACGATAGTGAAAA

CAATCACGAATGACCAAATTGAAGTTACTAATGCTACTGAGCTGGTTC

AGAATTCCTCAATAGGTGAAATATGCGACAGTCCTCATCAGATCCTTG

ATGGAGAAAACTGCACACTAATAGATGCTCTATTGGGAGACCCTCAG

TGTGATGGCTTTCAAAATAAGAAATGGGACCTTTTTGTTGAACGAAGC

AAAGCCTACAGCAACTGTTACCCTTATGATGTGCCGGATTATGCCTC

CCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG

AAAGCTTCAATTGGGCTGGAGTCACTCAAAACGGAACAAGTTCTGCT

TGCATAAGGGGATCTAATAGTAGTTTCTTTAGTAGATTAAATTGGTTG

ACCCACTTAAACTTCAAGTACCCAGCATTGAACGTGACTATGCCAAAC

AATGAACAATTTGACAAATTGTACATTTGGGGGGTTCACCACCCGGG

TACGGACAAGGACCAAATCTTCCTGTATGCTCAATCATCAGGAAGAA

TCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCGAATATC

GGATCTAGACCCAGAATAAGGAATATCCCTAGCAGAATAAGCATCTA

TTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAG

GGAATCTAATTGCTCCTAGGGGTTACTTCAAAATACGAAGTGGGAAA

AGCTCAATAATGAGATCAGATGCACCCATTGGCAAATGCAAGTCTGA

ATGCATCACTCCAAATGGAAGCATTCCCAATGACAAACCATTCCAAAA

TGTAAACAGGATCACATACGGGGCCTGTCCCAGATATGTTAAGCAAA

GCACTCTGAAATTGGCAACAGGAATGCGAAATGTACCAGAGAAACAA

ACTAGAGGCATATTTGGCGCAATAGCGGGTTTCATAGAAAATGGTTG

GGAGGGAATGGTGGATGGTTGGTACGGTTTCAGGCATCAAAATTCTG

AGGGAAGAGGACAAGCAGCAGATCTCAAAAGCACTCAAGCAGCAAT

CGATCAAATCAATGGGAAGCTGAATCGATTGATCGGGAAAACCAACG

AGAAATTCCATCAGATTGAGAAAGAATTCTCAGAAGTAGAAGGGAGA

ATTCAGGACCTTGAGAAATATGTTGAGGACACAAAAATAGATCTCTGG

TCATACAACGCAGAGCTTCTTGTTGCCCTGGAAAACCAACATACAATT

GATCTAACTGACTCAGAAATGAACAAACTGTTTGAAAAAACAAAGAAG

CAACTGAGGGAAAATGCTGAGGATATGGGCAATGGTTGTTTCAAAAT

ATACCACAAATGTGACAATGCCTGCATAGGATCAATCAGAAATGGAA

CTTATGACCACGATGTATACAGGGATGAAGCATTAAACAACCGGTTC

CAGATCAAGGGAGTTGAGCTGAAGTCAGGGTACAAAGATTGGATCCT

ATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTG

GGGTTCATCATGTGGGCCTGCCAAAAGGGCAACATTAGGTGCAACAT

TTGCATTTGAGTGCATTAATTAAAAACACCCTTGTTTCTACTGGAAGC

GGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGA

AAACCCCGGGCCTATTTTCTAATATCCACAAAATGAAGGCAATAATTG

TACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGG

ATAACATCGTCAAACTCACCACATGTCGTCAAAACTGCTACTCAAGG

GGAGGTCAACGTGACCGGTGTAATACCACTGACAACAACACCCACCA

AATCTCATTTTGCAAATCTCAAAGGAACAGAAACCAGGGGGAAACTAT

GCCCAAAATGCCTCAACTGCACAGATCTGGATGTAGCCTTGGGCAGA

CCAAAATGCACAGGGAAAATACCCTCTGCAAGGGTTTCAATACTCCA

TGAAGTCAGACCTGTTACATCTGGGTGCTTTCCTATAATGCACGATAG

AACAAAAATTAGACAGCTGCCTAACCTTCTCCGAGGATACGAACATG

TCAGGTTATCAACTCACAACGTTATCAATGCAAAAGATGCACCAGGAA

GACCCTACGAAATTGGAACCTCAGGGTCTTGCCCTAACATTACCAAT

GGAAACGGATTCTTCGCAACAATGGCTTGGGCCGTCCCAAAAAACAA

AACAGCAACAAATCCATTAACAATAGAAGTACCATACATTTGTACAGA

AGGAGAAGACCAAATTACCGTTTGGGGGTTCCACTCTGACAACGAGA

CCCAAATGGCAAAGCTCTATGGGGACTCAAAGCCCCAGAAGTTCACC

TCATCTGCCAACGGAGTGACCACACATTACGTTTCACAGATTGGTGG

CTTCCCAAATCAAACAGAAGACGGAGGACTACCACAAAGTGGCAGAA

TTGTTGTTGATTACATGGTGCAGAAATCTGGAAAAACAGGAACAATTA

CCTATCAAAGAGGTATTTTATTGCCTCAAAAAGTGTGGTGCGCAAGT

GGCAGGAGCAAGGTAATAAAAGGATCCTTGCCCTTAATTGGAGAAGC

AGATTGCCTCCATGAAAAATACGGTGGATTAAACAAAAGCAAGCCTTA

CTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG

TGAAAACACCCTTGAAGCTGGCCAATGGAACCAAATATAGACCCCCT

GCAAAACTATTAAAGGAAAGAGGTTTCTTCGGAGCCATTGCTGGTTT

CTTAGAGGGAGGATGGGAAGGAATGATTGCAGGTTGGCACGGATAC

ACATCCCATGGGGCACATGGAGTAGCGGTGGCAGCTGACCTTAAGA

GCACTCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACTCTTTGA

GTGAGCTGGAAGTAAAGAATCTTCAAAGACTAAGCGGTGCCATGGAT

GAACTCCACAACGAAATACTAGAACTAGATGAGAAAGTGGATGATCT

CAGAGCTGACACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT

CCAATGAAGGAATAATAAACAGTGAAGATGAACATCTCTTGGCGCTT

GAAAGAAAGCTGAAGAAAATGCTGGGCCCCTCTGCTGTAGAGATAG

GGAATGGATGCTTTGAAACCAAACACAAGTGCAACCAGACCTGCCTC

GACAGAATAGCTGCTGGTACCTTTGATGCAGGAGAATTTTCTCTCCC

CACCTTTGATTCACTGAATATTACTGCTGCATCTTTAAATGACGACGG

ATTGGACAATCATACTATACTGCTTTACTACTCAACTGCTGCCTCCAG

TTTGGCTGTAACACTGATGATAGCTATCTTTGTTGTTTATATGGTCTC

CAGAGACAATGTTTCTTGCTCCATTTGTCTATAAGGAAAGTTAAGCCC

TGTATTTTCCTTTATTGTAGTGCTTGTTTGCTTGTTGTCATTACAAAGA

AACGTTATTGAAAAATTGATGACTCGAGCTGGTACTGCATGCACGCA

ATGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCCGAGTCTCCCCCG

ACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGCCCCACTCACC

ACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCAATGCAGCTC

AAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAA

CCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGT

TGGTCAATTTCGTGCCAGCCACACCCTGGAGCTAGCAAAAAAAA

Pan-CoV-Flu
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATAT
407

Vaccine #19
ATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTG

CoV-Wuhan
ACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTC

Spike(HexaPro)-
CCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAG

influenza (HA)
TATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATG

CCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTG

GCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTA

CATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCA

GTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAA

GTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAAT

CAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCA

AATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT

GGTTTAGTGAACCGTCAGATCGAGAATAAACTAGTATTCTTCTGGTCC

CCACAGACTCAGAGAGAACCCGCCACCATGTTCGTGTTCCTGGTGCT

GCTGCCCCTGGTGAGCAGCCAGTGCGTGAACCTGACCACCAGGACC

CAGCTGCCCCCCGCCTACACCAACAGCTTCACCAGGGGCGTGTACT

ACCCCGACAAGGTGTTCAGGAGCAGCGTGCTGCACAGCACCCAGGA

CCTGTTCCTGCCCTTCTTCAGCAACGTGACCTGGTTCCACGCCATCC

ACGTGAGCGGCACCAACGGCACCAAGAGGTTCGACAACCCCGTGCT

GCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGAGAAGAGCAAC

ATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAGCAAGACCC

AGAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGATCAAGGT

GTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTACTACC

ACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTACAG

CAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCTG

ATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGT

TCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCAC

ACCCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCC

TGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTT

CCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGAC

AGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGC

TACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCA

CCATCACCGACGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGAC

CAAGTGCACCCTGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAG

ACCAGCAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTTCC

CCAACATCACCAACCTGTGCCCCTTCGGCGAGGTGTTCAACGCCAC

CAGGTTCGCCAGCGTGTACGCCTGGAACAGGAAGAGGATCAGCAAC

TGCGTGGCCGACTACAGCGTGCTGTACAACAGCGCCAGCTTCAGCA

CCTTCAAGTGCTACGGCGTGAGCCCCACCAAGCTGAACGACCTGTG

CTTCACCAACGTGTACGCCGACAGCTTCGTGATCAGGGGCGACGAG

GTGAGGCAGATCGCCCCCGGCCAGACCGGCAAGATCGCCGACTACA

ACTACAAGCTGCCCGACGACTTCACCGGCTGCGTGATCGCCTGGAA

CAGCAACAACCTGGACAGCAAGGTGGGCGGCAACTACAACTACCTG

TACAGGCTGTTCAGGAAGAGCAACCTGAAGCCCTTCGAGAGGGACA

TCAGCACCGAGATCTACCAGGCCGGCAGCACCCCCTGCAACGGCGT

GGAGGGCTTCAACTGCTACTTCCCCCTGCAGAGCTACGGCTTCCAG

CCCACCAACGGCGTGGGCTACCAGCCCTACAGGGTGGTGGTGCTGA

GCTTCGAGCTGCTGCACGCCCCCGCCACCGTGTGCGGCCCCAAGAA

GAGCACCAACCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACG

GCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACAAGAAGTTCCT

GCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCACCGACGCC

GTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACCCCCTGCA

GCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACACCAGCAA

CCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGGTGCCC

GTGGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTGTACA

GCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGATCG

GCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCGG

CGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCGGC

AGCGCCAGCAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGA

GCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGC

CATCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCG

TGAGCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGG

CGACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTC

TGCACCCAGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAG

GACAAGAACACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACA

AGACCCCCCCCATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATC

CTGCCCGACCCCAGCAAGCCCAGCAAGAGGAGCCCCATCGAGGAC

CTGCTGTTCAACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGC

AGTACGGCGACTGCCTGGGCGACATCGCCGCCAGGGACCTGATCTG

CGCCCAGAAGTTCAACGGCCTGACCGTGCTGCCCCCCCTGCTGACC

GACGAGATGATCGCCCAGTACACCAGCGCCCTGCTGGCCGGCACCA

TCACCAGCGGCTGGACCTTCGGCGCCGGCCCCGCCCTGCAGATCC

CCTTCCCCATGCAGATGGCCTACAGGTTCAACGGCATCGGCGTGAC

CCAGAACGTGCTGTACGAGAACCAGAAGCTGATCGCCAACCAGTTCA

ACAGCGCCATCGGCAAGATCCAGGACAGCCTGAGCAGCACCCCCAG

CGCCCTGGGCAAGCTGCAGGACGTGGTGAACCAGAACGCCCAGGC

CCTGAACACCCTGGTGAAGCAGCTGAGCAGCAACTTCGGCGCCATC

AGCAGCGTGCTGAACGACATCCTGAGCAGGCTGGACCCCCCCGAGG

CCGAGGTGCAGATCGACAGGCTGATCACCGGCAGGCTGCAGAGCCT

GCAGACCTACGTGACCCAGCAGCTGATCAGGGCCGCCGAGATCAGG

GCCAGCGCCAACCTGGCCGCCACCAAGATGAGCGAGTGCGTGCTG

GGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGGGCTACCACCTGA

TGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTGTTCCTGCACGT

GACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACCGCCCCCGCC

ATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGGCGTGTTCG

TGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTTCTACGA

GCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAACTGC

GACGTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCTGC

AGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATC

AACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACG

AGGTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCT

GGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTG

GGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCT

GTGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGC

TGCGGCAGCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTG

CTGAAGGGCGTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACT

TCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCT

ATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAAT

GCAGACACATTATGTATAGGTTATCATGCGAACAACTCAACAGACACT

GTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC

CTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGAGGGGT

AGCCCCATTGCATTTGGGTAAATGTAACATTGCTGGCTGGATCCTGG

GAAATCCAGAGTGTGAATCACTCTCCACAGCAAGCTCATGGTCCTAC

ATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAGGAGA

TTTCATCGATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTCAT

CATTTGAAAGGTTTGAGATATTCCCCAAGACAAGTTCATGGCCCAATC

ATGACTCGAACAAAGGTGTAACGACAGCATGTCCTCATGCTGGAGCA

AAAAGCTTCTACAAAAATTTAATATGGCTAGTTAAAAAAGGAAATTCAT

ACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGGGAAAGAAGTC

CTCGTGCTATGGGGCATTCACCATCCACCTACTAGTGCTGACCAACA

AAGTCTCTATCAGAATGCAGATGCATATGTTTTTGTGGGGACATCAAG

ATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGGCCCAAAGTGA

GGGATCAAGAAGGGAGAATGAACTATTACTGGACACTAGTAGAGCCG

GGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGTGGTACCGAG

ATATGCATTCGCAATGGAAAGAAATGCTGGATCTGGTATTATCATTTC

AGATACACCAGTCCACGATTGCAATACAACTTGTCAGACACCCAAGG

GTGCTATAAACACCAGCCTCCCATTTCAGAACATACATCCGATCACAA

TTGGAAAATGTCCAAAATATGTAAAAAGCACAAAATTGAGACTGGCCA

CAGGATTGAGGAATGTCCCGTCCATTCAATCTAGAGGCCTATTTGGG

GCCATTGCCGGTTTCATTGAAGGGGGGTGGACAGGGATGGTAGATG

GATGGTACGGTTATCACCATCAAAATGAGCAGGGGTCAGGATATGCA

GCCGACCTGAAGAGCACACAGAATGCCATTGACGAGATTACTAACAA

AGTAAACTCTGTTATTGAAAAAATGAATACACAGTTCACAGCAGTAGG

TAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGAATTTAAATAAAAA

AGTTGATGATGGTTTCCTGGACATTTGGACTTACAATGCCGAACTGTT

GGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAAGGT

GAAGAACTTATATGAAAAGGTAAGAAGCCAGTTAAAAAACAATGCCAA

GGAAATTGGAAACGGCTGCTTTGAATTTTACCACAAATGTGATAACAC

GTGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACT

CAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGGTAAAGCTG

GAATCAACAAGGATTTACCAGATTTTGGCGATCTATTCAACCGTCGCC

AGTTCATTGGTACTGGTAGTCTCCCTGGGGGCAATCAGTTTCTGGAT

GTGCTCTAATGGGTCTCTACAGTGTAGAATATGTATTTAAGGAAGCG

GAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAA

AACCCCGGGCCTAGCAAAAGCAGGGGATAATTCTATTAACCATGAAG

ACTATCATTGCTTTGAGCTACATTCTATGTCTGGTTTTCGCTCAAAAA

CTTCCTGGAAATGACAATAGCACTGCAACGCTGTGCCTTGGGCACCA

TGCAGTACCAAACGGAACGATAGTGAAAACAATCACGAATGACCAAA

TTGAAGTTACTAATGCTACTGAGCTGGTTCAGAATTCCTCAATAGGTG

AAATATGCGACAGTCCTCATCAGATCCTTGATGGAGAAAACTGCACA

CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAAT

AAGAAATGGGACCTTTTTGTTGAACGAAGCAAAGCCTACAGCAACTG

TTACCCTTATGATGTGCCGGATTATGCCTCCCTTAGGTCACTAGTTGC

CTCATCCGGCACACTGGAGTTTAACAATGAAAGCTTCAATTGGGCTG

GAGTCACTCAAAACGGAACAAGTTCTGCTTGCATAAGGGGATCTAAT

AGTAGTTTCTTTAGTAGATTAAATTGGTTGACCCACTTAAACTTCAAGT

ACCCAGCATTGAACGTGACTATGCCAAACAATGAACAATTTGACAAAT

TGTACATTTGGGGGGTTCACCACCCGGGTACGGACAAGGACCAAAT

CTTCCTGTATGCTCAATCATCAGGAAGAATCACAGTATCTACCAAAAG

AAGCCAACAAGCTGTAATCCCGAATATCGGATCTAGACCCAGAATAA

GGAATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAACCG

GGAGACATACTTTTGATTAACAGCACAGGGAATCTAATTGCTCCTAG

GGGTTACTTCAAAATACGAAGTGGGAAAAGCTCAATAATGAGATCAG

ATGCACCCATTGGCAAATGCAAGTCTGAATGCATCACTCCAAATGGA

AGCATTCCCAATGACAAACCATTCCAAAATGTAAACAGGATCACATAC

GGGGCCTGTCCCAGATATGTTAAGCAAAGCACTCTGAAATTGGCAAC

AGGAATGCGAAATGTACCAGAGAAACAAACTAGAGGCATATTTGGCG

CAATAGCGGGTTTCATAGAAAATGGTTGGGAGGGAATGGTGGATGGT

TGGTACGGTTTCAGGCATCAAAATTCTGAGGGAAGAGGACAAGCAGC

AGATCTCAAAAGCACTCAAGCAGCAATCGATCAAATCAATGGGAAGC

TGAATCGATTGATCGGGAAAACCAACGAGAAATTCCATCAGATTGAG

AAAGAATTCTCAGAAGTAGAAGGGAGAATTCAGGACCTTGAGAAATA

TGTTGAGGACACAAAAATAGATCTCTGGTCATACAACGCAGAGCTTC

TTGTTGCCCTGGAAAACCAACATACAATTGATCTAACTGACTCAGAAA

TGAACAAACTGTTTGAAAAAACAAAGAAGCAACTGAGGGAAAATGCT

GAGGATATGGGCAATGGTTGTTTCAAAATATACCACAAATGTGACAAT

GCCTGCATAGGATCAATCAGAAATGGAACTTATGACCACGATGTATA

CAGGGATGAAGCATTAAACAACCGGTTCCAGATCAAGGGAGTTGAGC

TGAAGTCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT

CATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCATCATGTGGGCCT

GCCAAAAGGGCAACATTAGGTGCAACATTTGCATTTGAGTGCATTAAT

TAAAAACACCCTTGTTTCTACTGGAAGCGGAGCCACGAACTTCTCTCT

GTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTATTTTCT

AATATCCACAAAATGAAGGCAATAATTGTACTACTCATGGTAGTAACA

TCCAATGCAGATCGAATCTGCACTGGGATAACATCGTCAAACTCACC

ACATGTCGTCAAAACTGCTACTCAAGGGGAGGTCAACGTGACCGGT

GTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC

AAAGGAACAGAAACCAGGGGGAAACTATGCCCAAAATGCCTCAACTG

CACAGATCTGGATGTAGCCTTGGGCAGACCAAAATGCACAGGGAAAA

TACCCTCTGCAAGGGTTTCAATACTCCATGAAGTCAGACCTGTTACAT

CTGGGTGCTTTCCTATAATGCACGATAGAACAAAAATTAGACAGCTG

CCTAACCTTCTCCGAGGATACGAACATGTCAGGTTATCAACTCACAA

CGTTATCAATGCAAAAGATGCACCAGGAAGACCCTACGAAATTGGAA

CCTCAGGGTCTTGCCCTAACATTACCAATGGAAACGGATTCTTCGCA

ACAATGGCTTGGGCCGTCCCAAAAAACAAAACAGCAACAAATCCATT

AACAATAGAAGTACCATACATTTGTACAGAAGGAGAAGACCAAATTAC

CGTTTGGGGGTTCCACTCTGACAACGAGACCCAAATGGCAAAGCTCT

ATGGGGACTCAAAGCCCCAGAAGTTCACCTCATCTGCCAACGGAGT

GACCACACATTACGTTTCACAGATTGGTGGCTTCCCAAATCAAACAG

AAGACGGAGGACTACCACAAAGTGGCAGAATTGTTGTTGATTACATG

GTGCAGAAATCTGGAAAAACAGGAACAATTACCTATCAAAGAGGTATT

TTATTGCCTCAAAAAGTGTGGTGCGCAAGTGGCAGGAGCAAGGTAAT

AAAAGGATCCTTGCCCTTAATTGGAGAAGCAGATTGCCTCCATGAAA

AATACGGTGGATTAAACAAAAGCAAGCCTTACTACACAGGGGAACAT

GCAAAGGCCATAGGAAATTGCCCAATATGGGTGAAAACACCCTTGAA

GCTGGCCAATGGAACCAAATATAGACCCCCTGCAAAACTATTAAAGG

AAAGAGGTTTCTTCGGAGCCATTGCTGGTTTCTTAGAGGGAGGATGG

GAAGGAATGATTGCAGGTTGGCACGGATACACATCCCATGGGGCAC

ATGGAGTAGCGGTGGCAGCTGACCTTAAGAGCACTCAAGAGGCCAT

AAACAAGATAACAAAAAATCTCAACTCTTTGAGTGAGCTGGAAGTAAA

GAATCTTCAAAGACTAAGCGGTGCCATGGATGAACTCCACAACGAAA

TACTAGAACTAGATGAGAAAGTGGATGATCTCAGAGCTGACACAATA

AGCTCACAAATAGAACTCGCAGTCCTGCTTTCCAATGAAGGAATAATA

AACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAAGAA

AATGCTGGGCCCCTCTGCTGTAGAGATAGGGAATGGATGCTTTGAAA

CCAAACACAAGTGCAACCAGACCTGCCTCGACAGAATAGCTGCTGGT

ACCTTTGATGCAGGAGAATTTTCTCTCCCCACCTTTGATTCACTGAAT

ATTACTGCTGCATCTTTAAATGACGACGGATTGGACAATCATACTATA

CTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACACTGATG

ATAGCTATCTTTGTTGTTTATATGGTCTCCAGAGACAATGTTTCTTGCT

CCATTTGTCTATAAGGAAAGTTAAGCCCTGTATTTTCCTTTATTGTAGT

GCTTGTTTGCTTGTTGTCATTACAAAGAAACGTTATTGAAAAATTGAT

GACTCGAGCTGGTACTGCATGCACGCAATGCTAGCTGCCCCTTTCCC

GTCCTGGGTACCCCGAGTCTCCCCCGACCTCGGGTCCCAGGTATGC

TCCCACCTCCACCTGCCCCACTCACCACCTCTGCTAGTTCCAGACAC

CTCCCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACA

CCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGT

TTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCC

ACACCCTGGAGCTAGCAAAAAAAA

Pan-CoV-Flu
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATAT
408

Vaccine #20
ATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTG

CoV-Wuhan
ACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTC

Spike(2 proline
CCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAG

substitution)-
TATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATG

Influenza (HA)
CCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTG

GCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTA

CATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCA

GTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAA

GTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAAT

CAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCA

AATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT

GGTTTAGTGAACCGTCAGATCGAGAATAAACTAGTATTCTTCTGGTCC

CCACAGACTCAGAGAGAACCCGCCACCATGTTCGTGTTCCTGGTGCT

GCTGCCCCTGGTGAGCAGCCAGTGCGTGAACCTGACCACCAGGACC

CAGCTGCCCCCCGCCTACACCAACAGCTTCACCAGGGGCGTGTACT

ACCCCGACAAGGTGTTCAGGAGCAGCGTGCTGCACAGCACCCAGGA

CCTGTTCCTGCCCTTCTTCAGCAACGTGACCTGGTTCCACGCCATCC

ACGTGAGCGGCACCAACGGCACCAAGAGGTTCGACAACCCCGTGCT

GCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGAGAAGAGCAAC

ATCATCAGGGGCTGGATCTTCGGCACCACCCTGGACAGCAAGACCC

AGAGCCTGCTGATCGTGAACAACGCCACCAACGTGGTGATCAAGGT

GTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGGCGTGTACTACC

ACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCAGGGTGTACAG

CAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCAGCCCTTCCTG

ATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAACCTGAGGGAGT

TCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTACAGCAAGCAC

ACCCCCATCAACCTGGTGAGGGACCTGCCCCAGGGCTTCAGCGCCC

TGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACATCACCAGGTT

CCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGACCCCCGGCGAC

AGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTACTACGTGGGC

TACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAACGAGAACGGCA

CCATCACCGACGCCGTGGACTGCGCCCTGGACCCCCTGAGCGAGAC

CAAGTGCACCCTGAAGAGCTTCACCGTGGAGAAGGGCATCTACCAG

ACCAGCAACTTCAGGGTGCAGCCCACCGAGAGCATCGTGAGGTTCC

CCAACATCACCAACCTGTGCCCCTTCGGCGAGGTGTTCAACGCCAC

CAGGTTCGCCAGCGTGTACGCCTGGAACAGGAAGAGGATCAGCAAC

TGCGTGGCCGACTACAGCGTGCTGTACAACAGCGCCAGCTTCAGCA

CCTTCAAGTGCTACGGCGTGAGCCCCACCAAGCTGAACGACCTGTG

CTTCACCAACGTGTACGCCGACAGCTTCGTGATCAGGGGCGACGAG

GTGAGGCAGATCGCCCCCGGCCAGACCGGCAAGATCGCCGACTACA

ACTACAAGCTGCCCGACGACTTCACCGGCTGCGTGATCGCCTGGAA

CAGCAACAACCTGGACAGCAAGGTGGGCGGCAACTACAACTACCTG

TACAGGCTGTTCAGGAAGAGCAACCTGAAGCCCTTCGAGAGGGACA

TCAGCACCGAGATCTACCAGGCCGGCAGCACCCCCTGCAACGGCGT

GGAGGGCTTCAACTGCTACTTCCCCCTGCAGAGCTACGGCTTCCAG

CCCACCAACGGCGTGGGCTACCAGCCCTACAGGGTGGTGGTGCTGA

GCTTCGAGCTGCTGCACGCCCCCGCCACCGTGTGCGGCCCCAAGAA

GAGCACCAACCTGGTGAAGAACAAGTGCGTGAACTTCAACTTCAACG

GCCTGACCGGCACCGGCGTGCTGACCGAGAGCAACAAGAAGTTCCT

GCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACACCACCGACGCC

GTGAGGGACCCCCAGACCCTGGAGATCCTGGACATCACCCCCTGCA

GCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACCAACACCAGCAA

CCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACCGAGGTGCCC

GTGGCCATCCACGCCGACCAGCTGACCCCCACCTGGAGGGTGTACA

GCACCGGCAGCAACGTGTTCCAGACCAGGGCCGGCTGCCTGATCG

GCGCCGAGCACGTGAACAACAGCTACGAGTGCGACATCCCCATCGG

CGCCGGCATCTGCGCCAGCTACCAGACCCAGACCAACAGCCCCAGG

AGGGCCAGGAGCGTGGCCAGCCAGAGCATCATCGCCTACACCATGA

GCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAACAACAGCATCGC

CATCCCCACCAACTTCACCATCAGCGTGACCACCGAGATCCTGCCCG

TGAGCATGACCAAGACCAGCGTGGACTGCACCATGTACATCTGCGG

CGACAGCACCGAGTGCAGCAACCTGCTGCTGCAGTACGGCAGCTTC

TGCACCCAGCTGAACAGGGCCCTGACCGGCATCGCCGTGGAGCAG

GACAAGAACACCCAGGAGGTGTTCGCCCAGGTGAAGCAGATCTACA

AGACCCCCCCCATCAAGGACTTCGGCGGCTTCAACTTCAGCCAGATC

CTGCCCGACCCCAGCAAGCCCAGCAAGAGGAGCTTCATCGAGGACC

TGCTGTTCAACAAGGTGACCCTGGCCGACGCCGGCTTCATCAAGCA

GTACGGCGACTGCCTGGGCGACATCGCCGCCAGGGACCTGATCTGC

GCCCAGAAGTTCAACGGCCTGACCGTGCTGCCCCCCCTGCTGACCG

ACGAGATGATCGCCCAGTACACCAGCGCCCTGCTGGCCGGCACCAT

CACCAGCGGCTGGACCTTCGGCGCCGGCGCCGCCCTGCAGATCCC

CTTCGCCATGCAGATGGCCTACAGGTTCAACGGCATCGGCGTGACC

CAGAACGTGCTGTACGAGAACCAGAAGCTGATCGCCAACCAGTTCAA

CAGCGCCATCGGCAAGATCCAGGACAGCCTGAGCAGCACCGCCAGC

GCCCTGGGCAAGCTGCAGGACGTGGTGAACCAGAACGCCCAGGCC

CTGAACACCCTGGTGAAGCAGCTGAGCAGCAACTTCGGCGCCATCA

GCAGCGTGCTGAACGACATCCTGAGCAGGCTGGACCCCCCCGAGG

CCGAGGTGCAGATCGACAGGCTGATCACCGGCAGGCTGCAGAGCCT

GCAGACCTACGTGACCCAGCAGCTGATCAGGGCCGCCGAGATCAGG

GCCAGCGCCAACCTGGCCGCCACCAAGATGAGCGAGTGCGTGCTG

GGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGGGCTACCACCTGA

TGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTGTTCCTGCACGT

GACCTACGTGCCCGCCCAGGAGAAGAACTTCACCACCGCCCCCGCC

ATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGAGGGCGTGTTCG

TGAGCAACGGCACCCACTGGTTCGTGACCCAGAGGAACTTCTACGA

GCCCCAGATCATCACCACCGACAACACCTTCGTGAGCGGCAACTGC

GACGTGGTGATCGGCATCGTGAACAACACCGTGTACGACCCCCTGC

AGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACAAGTACTTCAA

GAACCACACCAGCCCCGACGTGGACCTGGGCGACATCAGCGGCATC

AACGCCAGCGTGGTGAACATCCAGAAGGAGATCGACAGGCTGAACG

AGGTGGCCAAGAACCTGAACGAGAGCCTGATCGACCTGCAGGAGCT

GGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGTACATCTGGCTG

GGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTGACCATCATGCT

GTGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGGGCTGCTGCAGC

TGCGGCAGCTGCTGCAAGTTCGACGAGGACGACAGCGAGCCCGTG

CTGAAGGGCGTGAAGCTGCACTACACCGGAAGCGGAGCCACGAACT

TCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCT

ATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAAT

GCAGACACATTATGTATAGGTTATCATGCGAACAACTCAACAGACACT

GTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC

CTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGAGGGGT

AGCCCCATTGCATTTGGGTAAATGTAACATTGCTGGCTGGATCCTGG

GAAATCCAGAGTGTGAATCACTCTCCACAGCAAGCTCATGGTCCTAC

ATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAGGAGA

TTTCATCGATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTCAT

CATTTGAAAGGTTTGAGATATTCCCCAAGACAAGTTCATGGCCCAATC

ATGACTCGAACAAAGGTGTAACGACAGCATGTCCTCATGCTGGAGCA

AAAAGCTTCTACAAAAATTTAATATGGCTAGTTAAAAAAGGAAATTCAT

ACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGGGAAAGAAGTC

CTCGTGCTATGGGGCATTCACCATCCACCTACTAGTGCTGACCAACA

AAGTCTCTATCAGAATGCAGATGCATATGTTTTTGTGGGGACATCAAG

ATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGGCCCAAAGTGA

GGGATCAAGAAGGGAGAATGAACTATTACTGGACACTAGTAGAGCCG

GGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGTGGTACCGAG

ATATGCATTCGCAATGGAAAGAAATGCTGGATCTGGTATTATCATTTC

AGATACACCAGTCCACGATTGCAATACAACTTGTCAGACACCCAAGG

GTGCTATAAACACCAGCCTCCCATTTCAGAACATACATCCGATCACAA

TTGGAAAATGTCCAAAATATGTAAAAAGCACAAAATTGAGACTGGCCA

CAGGATTGAGGAATGTCCCGTCCATTCAATCTAGAGGCCTATTTGGG

GCCATTGCCGGTTTCATTGAAGGGGGGTGGACAGGGATGGTAGATG

GATGGTACGGTTATCACCATCAAAATGAGCAGGGGTCAGGATATGCA

GCCGACCTGAAGAGCACACAGAATGCCATTGACGAGATTACTAACAA

AGTAAACTCTGTTATTGAAAAAATGAATACACAGTTCACAGCAGTAGG

TAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGAATTTAAATAAAAA

AGTTGATGATGGTTTCCTGGACATTTGGACTTACAATGCCGAACTGTT

GGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAAGGT

GAAGAACTTATATGAAAAGGTAAGAAGCCAGTTAAAAAACAATGCCAA

GGAAATTGGAAACGGCTGCTTTGAATTTTACCACAAATGTGATAACAC

GTGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACT

CAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGGTAAAGCTG

GAATCAACAAGGATTTACCAGATTTTGGCGATCTATTCAACCGTCGCC

AGTTCATTGGTACTGGTAGTCTCCCTGGGGGCAATCAGTTTCTGGAT

GTGCTCTAATGGGTCTCTACAGTGTAGAATATGTATTTAAGGAAGCG

GAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAA

AACCCCGGGCCTAGCAAAAGCAGGGGATAATTCTATTAACCATGAAG

ACTATCATTGCTTTGAGCTACATTCTATGTCTGGTTTTCGCTCAAAAA

CTTCCTGGAAATGACAATAGCACTGCAACGCTGTGCCTTGGGCACCA

TGCAGTACCAAACGGAACGATAGTGAAAACAATCACGAATGACCAAA

TTGAAGTTACTAATGCTACTGAGCTGGTTCAGAATTCCTCAATAGGTG

AAATATGCGACAGTCCTCATCAGATCCTTGATGGAGAAAACTGCACA

CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAAT

AAGAAATGGGACCTTTTTGTTGAACGAAGCAAAGCCTACAGCAACTG

TTACCCTTATGATGTGCCGGATTATGCCTCCCTTAGGTCACTAGTTGC

CTCATCCGGCACACTGGAGTTTAACAATGAAAGCTTCAATTGGGCTG

GAGTCACTCAAAACGGAACAAGTTCTGCTTGCATAAGGGGATCTAAT

AGTAGTTTCTTTAGTAGATTAAATTGGTTGACCCACTTAAACTTCAAGT

ACCCAGCATTGAAGGTGACTATGCCAAACAATGAACAATTTGACAAAT

TGTACATTTGGGGGGTTCACCACCCGGGTACGGACAAGGACCAAAT

CTTCCTGTATGCTCAATCATCAGGAAGAATCACAGTATCTACCAAAAG

AAGCCAACAAGCTGTAATCCCGAATATCGGATCTAGACCCAGAATAA

GGAATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAACCG

GGAGACATACTTTTGATTAACAGCACAGGGAATCTAATTGCTCCTAG

GGGTTACTTCAAAATACGAAGTGGGAAAAGCTCAATAATGAGATCAG

ATGCACCCATTGGCAAATGCAAGTCTGAATGCATCACTCCAAATGGA

AGCATTCCCAATGACAAACCATTCCAAAATGTAAACAGGATCACATAC

GGGGCCTGTCCCAGATATGTTAAGCAAAGCACTCTGAAATTGGCAAC

AGGAATGCGAAATGTACCAGAGAAACAAACTAGAGGCATATTTGGCG

CAATAGCGGGTTTCATAGAAAATGGTTGGGAGGGAATGGTGGATGGT

TGGTACGGTTTCAGGCATCAAAATTCTGAGGGAAGAGGACAAGCAGC

AGATCTCAAAAGCACTCAAGCAGCAATCGATCAAATCAATGGGAAGC

TGAATCGATTGATCGGGAAAACCAACGAGAAATTCCATCAGATTGAG

AAAGAATTCTCAGAAGTAGAAGGGAGAATTCAGGACCTTGAGAAATA

TGTTGAGGACACAAAAATAGATCTCTGGTCATACAACGCAGAGCTTC

TTGTTGCCCTGGAAAACCAACATACAATTGATCTAACTGACTCAGAAA

TGAACAAACTGTTTGAAAAAACAAAGAAGCAACTGAGGGAAAATGCT

GAGGATATGGGCAATGGTTGTTTCAAAATATACCACAAATGTGACAAT

GCCTGCATAGGATCAATCAGAAATGGAACTTATGACCACGATGTATA

CAGGGATGAAGCATTAAACAACCGGTTCCAGATCAAGGGAGTTGAGC

TGAAGTCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT

CATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCATCATGTGGGCCT

GCCAAAAGGGCAACATTAGGTGCAACATTTGCATTTGAGTGCATTAAT

TAAAAACACCCTTGTTTCTACTGGAAGCGGAGCCACGAACTTCTCTCT

GTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTATTTTCT

AATATCCACAAAATGAAGGCAATAATTGTACTACTCATGGTAGTAACA

TCCAATGCAGATCGAATCTGCACTGGGATAACATCGTCAAACTCACC

ACATGTCGTCAAAACTGCTACTCAAGGGGAGGTCAACGTGACCGGT

GTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC

AAAGGAACAGAAACCAGGGGGAAACTATGCCCAAAATGCCTCAACTG

CACAGATCTGGATGTAGCCTTGGGCAGACCAAAATGCACAGGGAAAA

TACCCTCTGCAAGGGTTTCAATACTCCATGAAGTCAGACCTGTTACAT

CTGGGTGCTTTCCTATAATGCACGATAGAACAAAAATTAGACAGCTG

CCTAACCTTCTCCGAGGATACGAACATGTCAGGTTATCAACTCACAA

CGTTATCAATGCAAAAGATGCACCAGGAAGACCCTACGAAATTGGAA

CCTCAGGGTCTTGCCCTAACATTACCAATGGAAACGGATTCTTCGCA

ACAATGGCTTGGGCCGTCCCAAAAAACAAAACAGCAACAAATCCATT

AACAATAGAAGTACCATACATTTGTACAGAAGGAGAAGACCAAATTAC

CGTTTGGGGGTTCCACTCTGACAACGAGACCCAAATGGCAAAGCTCT

ATGGGGACTCAAAGCCCCAGAAGTTCACCTCATCTGCCAACGGAGT

GACCACACATTACGTTTCACAGATTGGTGGCTTCCCAAATCAAACAG

AAGACGGAGGACTACCACAAAGTGGCAGAATTGTTGTTGATTACATG

GTGCAGAAATCTGGAAAAACAGGAACAATTACCTATCAAAGAGGTATT

TTATTGCCTCAAAAAGTGTGGTGCGCAAGTGGCAGGAGCAAGGTAAT

AAAAGGATCCTTGCCCTTAATTGGAGAAGCAGATTGCCTCCATGAAA

AATACGGTGGATTAAACAAAAGCAAGCCTTACTACACAGGGGAACAT

GCAAAGGCCATAGGAAATTGCCCAATATGGGTGAAAACACCCTTGAA

GCTGGCCAATGGAACCAAATATAGACCCCCTGCAAAACTATTAAAGG

AAAGAGGTTTCTTCGGAGCCATTGCTGGTTTCTTAGAGGGAGGATGG

GAAGGAATGATTGCAGGTTGGCACGGATACACATCCCATGGGGCAC

ATGGAGTAGCGGTGGCAGCTGACCTTAAGAGCACTCAAGAGGCCAT

AAACAAGATAACAAAAAATCTCAACTCTTTGAGTGAGCTGGAAGTAAA

GAATCTTCAAAGACTAAGCGGTGCCATGGATGAACTCCACAACGAAA

TACTAGAACTAGATGAGAAAGTGGATGATCTCAGAGCTGACACAATA

AGCTCACAAATAGAACTCGCAGTCCTGCTTTCCAATGAAGGAATAATA

AACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAAGAA

AATGCTGGGCCCCTCTGCTGTAGAGATAGGGAATGGATGCTTTGAAA

CCAAACACAAGTGCAACCAGACCTGCCTCGACAGAATAGCTGCTGGT

ACCTTTGATGCAGGAGAATTTTCTCTCCCCACCTTTGATTCACTGAAT

ATTACTGCTGCATCTTTAAATGACGACGGATTGGACAATCATACTATA

CTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACACTGATG

ATAGCTATCTTTGTTGTTTATATGGTCTCCAGAGACAATGTTTCTTGCT

CCATTTGTCTATAAGGAAAGTTAAGCCCTGTATTTTCCTTTATTGTAGT

GCTTGTTTGCTTGTTGTCATTACAAAGAAACGTTATTGAAAAATTGAT

GACTCGAGCTGGTACTGCATGCACGCAATGCTAGCTGCCCCTTTCCC

GTCCTGGGTACCCCGAGTCTCCCCCGACCTCGGGTCCCAGGTATGC

TCCCACCTCCACCTGCCCCACTCACCACCTCTGCTAGTTCCAGACAC

CTCCCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACA

CCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGT

TTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCC

ACACCCTGGAGCTAGCAAAAAAAA

Pan-CoV-Flu
CTCGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGT
409

Vaccine #21
CATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGG

CoV-Wuhan
TAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGAC

Spike(HexaPro)-
GTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCA

Influenza (HA)
TTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAG

TACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATG

ACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGG

GACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACC

ATGGTCGAGGTGAGCCCCACGTTCTGCTTCACTCTCCCCATCTCCCC

CCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTTTGT

GCAGCGATGGGGGCGGGGGGGGGGGGGGGGCGCGCGCCAGGCG

GGGCGGGGCGGGGCGAGGGGCGGGGCGGGGCGAGGCGGAGAGG

TGCGGCGGCAGCCAATCAGAGCGGCGCGCTCCGAAAGTTTCCTTTT

ATGGCGAGGCGGCGGCGGCGGCGGCCCTATAAAAAGCGAAGCGCG

CGGCGGGCGGGAGTCGCTGCGCGCTGCCTTCGCCCCGTGCCCCGC

TCCGCCGCCGCCTCGCGCCGCCCGCCCCGGCTCTGACTGACCGCG

TTACTCCCACAGGTGAGCGGGCGGGACGGCCCTTCTCCTCCGGGCT

GTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGC

GTGAAAGCCTTGAGGGGCTCCGGGAGGGCCCTTTGTGCGGGGGGA

GCGGCTCGGGGGGTGCGTGCGTGTGTGTGTGCGTGGGGAGCGCCG

CGTGCGGCTCCGCGCTGCCCGGCGGCTGTGAGCGCTGCGGGCGCG

GCGCGGGGCTTTGTGCGCTCCGCAGTGTGCGCGAGGGGAGCGCGG

CCGGGGGCGGTGCCCCGCGGTGCGGGGGGGGCTGCGAGGGGAAC

AAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGGGTGAGCAGGGGGT

GTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTGCACCCCCCTCCC

CGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGGGCTCCGTACG

GGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGGTGGCGGCA

GGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCCGGGGAG

GGCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGCGGCTG

TCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCGTGC

GAGAGGGCGCAGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCGA

AATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGA

AGCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTC

GTGCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGG

CTGTCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGG

CGGGGTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGC

TAACCATGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGT

GCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAGAATTGGAGAATAA

ACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGAACCCGCCACCA

TGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGT

GAACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAGC

TTCACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCG

TGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTG

ACCTGGTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAGA

GGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGC

CAGCACCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACC

ACCCTGGACAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCA

CCAACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCC

CTTCCTGGGCGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGA

GCGAGTTCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTA

CGTGAGCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAAC

TTCAAGAACCTGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTT

CAAGATCTACAGCAAGCACACCCCCATCAACCTGGTGAGGGACCTG

CCCCAGGGCTTCAGCGCCCTGGAGCCCCTGGTGGACCTGCCCATCG

GCATCAACATCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAG

CTACCTGACCCCCGGCGACAGCAGCAGCGGCTGGACCGCCGGCGC

CGCCGCCTACTACGTGGGCTACCTGCAGCCCAGGACCTTCCTGCTG

AAGTACAACGAGAACGGCACCATCACCGACGCCGTGGACTGCGCCC

TGGACCCCCTGAGCGAGACCAAGTGCACCCTGAAGAGCTTCACCGT

GGAGAAGGGCATCTACCAGACCAGCAACTTCAGGGTGCAGCCCACC

GAGAGCATCGTGAGGTTCCCCAACATCACCAACCTGTGCCCCTTCG

GCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGTACGCCTGGAA

CAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTAC

AACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTGAGCCCCA

CCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTC

GTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCAGACC

GGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCACCG

GCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGGG

CGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAG

CACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTG

CAGAGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCT

ACAGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCAC

CGTGTGCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGC

GTGAACTTCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCG

AGAGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACAT

CGCCGACACCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGAT

CCTGGACATCACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACC

CCCGGCACCAACACCAGCAACCAGGTGGCCGTGCTGTACCAGGACG

TGAACTGCACCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGAC

CCCCACCTGGAGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACC

AGGGCCGGCTGCCTGATCGGCGCCGAGCACGTGAACAACAGCTAC

GAGTGCGACATCCCCATCGGCGCCGGCATCTGCGCCAGCTACCAGA

CCCAGACCAACAGCCCCGGCAGCGCCAGCAGCGTGGCCAGCCAGA

GCATCATCGCCTACACCATGAGCCTGGGCGCCGAGAACAGCGTGGC

CTACAGCAACAACAGCATCGCCATCCCCACCAACTTCACCATCAGCG

TGACCACCGAGATCCTGCCCGTGAGCATGACCAAGACCAGCGTGGA

CTGCACCATGTACATCTGCGGCGACAGCACCGAGTGCAGCAACCTG

CTGCTGCAGTACGGCAGCTTCTGCACCCAGCTGAACAGGGCCCTGA

CCGGCATCGCCGTGGAGCAGGACAAGAACACCCAGGAGGTGTTCGC

CCAGGTGAAGCAGATCTACAAGACCCCCCCCATCAAGGACTTCGGC

GGCTTCAACTTCAGCCAGATCCTGCCCGACCCCAGCAAGCCCAGCA

AGAGGAGCCCCATCGAGGACCTGCTGTTCAACAAGGTGACCCTGGC

CGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCTGGGCGACATC

GCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACGGCCTGACCG

TGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAGTACACCAG

CGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTTCGGCGC

CGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATGGCCTACAGG

TTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACCAGA

AGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGGA

CAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGT

GGTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTG

AGCAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGA

GCAGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGA

TCACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCT

GATCAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCAC

CAAGATGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTC

TGCGGCAAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCC

ACGGCGTGGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAA

GAACTTCACCACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCAC

TTCCCCAGGGAGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCG

TGACCCAGAGGAACTTCTACGAGCCCCAGATCATCACCACCGACAAC

ACCTTCGTGAGCGGCAACTGCGACGTGGTGATCGGCATCGTGAACA

ACACCGTGTACGACCCCCTGCAGCCCGAGCTGGACAGCTTCAAGGA

GGAGCTGGACAAGTACTTCAAGAACCACACCAGCCCCGACGTGGAC

CTGGGCGACATCAGCGGCATCAACGCCAGCGTGGTGAACATCCAGA

AGGAGATCGACAGGCTGAACGAGGTGGCCAAGAACCTGAACGAGAG

CCTGATCGACCTGCAGGAGCTGGGCAAGTACGAGCAGTACATCAAG

TGGCCCTGGTACATCTGGCTGGGCTTCATCGCCGGCCTGATCGCCA

TCGTGATGGTGACCATCATGCTGTGCTGCATGACCAGCTGCTGCAGC

TGCCTGAAGGGCTGCTGCAGCTGCGGCAGCTGCTGCAAGTTCGACG

AGGACGACAGCGAGCCCGTGCTGAAGGGCGTGAAGCTGCACTACAC

CGGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGAT

GTTGAAGAAAACCCCGGGCCTATGAAGGCAATACTAGTAGTTCTGCT

ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCA

TGCGAACAACTCAACAGACACTGTAGACACAGTACTAGAAAAGAATG

TAACAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGA

AACTATGCAAACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGT

AACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCTC

CACAGCAAGCTCATGGTCCTACATTGTGGAAACATCTAGTTCAGACA

ATGGAACGTGTTACCCAGGAGATTTCATCGATTATGAGGAGCTAAGA

GAGCAATTGAGCTCAGTGTCATCATTTGAAAGGTTTGAGATATTCCCC

AAGACAAGTTCATGGCCCAATCATGACTCGAACAAAGGTGTAACGAC

AGCATGTCCTCATGCTGGAGCAAAAAGCTTCTACAAAAATTTAATATG

GCTAGTTAAAAAAGGAAATTCATACCCAAAGCTCAGCAAATCCTACAT

TAATGATAAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTCACCATC

CACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCA

TATGTTTTTGTGGGGACATCAAGATACAGCAAGAAGTTCAAGCCGGA

AATAGCAATAAGGCCCAAAGTGAGGGATCAAGAAGGGAGAATGAACT

ATTACTGGACACTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCA

ACTGGAAATCTAGTGGTACCGAGATATGCATTCGCAATGGAAAGAAA

TGCTGGATCTGGTATTATCATTTCAGATACACCAGTCCACGATTGCAA

TACAACTTGTCAGACACCCAAGGGTGCTATAAACACCAGCCTCCCAT

TTCAGAACATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAA

AAAGCACAAAATTGAGACTGGCCACAGGATTGAGGAATGTCCCGTCC

ATTCAATCTAGAGGCCTATTTGGGGCCATTGCCGGTTTCATTGAAGG

GGGGTGGACAGGGATGGTAGATGGATGGTACGGTTATCACCATCAA

AATGAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACACAGA

ATGCCATTGACGAGATTACTAACAAAGTAAACTCTGTTATTGAAAAAA

TGAATACACAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAA

AAAAGAATAGAGAATTTAAATAAAAAAGTTGATGATGGTTTCCTGGAC

ATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGA

ACTTTGGACTACCACGATTCAAAGGTGAAGAACTTATATGAAAAGGTA

AGAAGCCAGTTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTT

TGAATTTTACCACAAATGTGATAACACGTGCATGGAAAGTGTCAAAAA

TGGGACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACA

GAGAAGAAATAGATGGGGTAAAGCTGGAATCAACAAGGATTTACCAG

ATTTTGGCGATCTATTCAACCGTCGCCAGTTCATTGGTACTGGTAGTC

TCCCTGGGGGCAATCAGTTTCTGGATGTGCTCTAATGGGTCTCTACA

GTGTAGAATATGTATTTAAGGAAGCGGAGCCACGAACTTCTCTCTGTT

AAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTAGCAAAAGC

AGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTTGAGCTACA

TTCTATGTCTGGTTTTCGCTCAAAAACTTCCTGGAAATGACAATAGCA

CTGCAACGCTGTGCCTTGGGCACCATGCAGTACCAAACGGAACGAT

AGTGAAAACAATCACGAATGACCAAATTGAAGTTACTAATGCTACTGA

GCTGGTTCAGAATTCCTCAATAGGTGAAATATGCGACAGTCCTCATC

AGATCCTTGATGGAGAAAACTGCACACTAATAGATGCTCTATTGGGA

GACCCTCAGTGTGATGGCTTTCAAAATAAGAAATGGGACCTTTTTGTT

GAACGAAGCAAAGCCTACAGCAACTGTTACCCTTATGATGTGCCGGA

TTATGCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGT

TTAACAATGAAAGCTTCAATTGGGCTGGAGTCACTCAAAACGGAACA

AGTTCTGCTTGCATAAGGGGATCTAATAGTAGTTTCTTTAGTAGATTA

AATTGGTTGACCCACTTAAACTTCAAGTACCCAGCATTGAACGTGACT

ATGCCAAACAATGAACAATTTGACAAATTGTACATTTGGGGGGTTCAC

CACCCGGGTACGGACAAGGACCAAATCTTCCTGTATGCTCAATCATC

AGGAAGAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCC

CGAATATCGGATCTAGACCCAGAATAAGGAATATCCCTAGCAGAATA

AGCATCTATTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAAC

AGCACAGGGAATCTAATTGCTCCTAGGGGTTACTTCAAAATACGAAG

TGGGAAAAGCTCAATAATGAGATCAGATGCACCCATTGGCAAATGCA

AGTCTGAATGCATCACTCCAAATGGAAGCATTCCCAATGACAAACCAT

TCCAAAATGTAAACAGGATCACATACGGGGCCTGTCCCAGATATGTT

AAGCAAAGCACTCTGAAATTGGCAACAGGAATGCGAAATGTACCAGA

GAAACAAACTAGAGGCATATTTGGCGCAATAGCGGGTTTCATAGAAA

ATGGTTGGGAGGGAATGGTGGATGGTTGGTACGGTTTCAGGCATCA

AAATTCTGAGGGAAGAGGACAAGCAGCAGATCTCAAAAGCACTCAAG

CAGCAATCGATCAAATCAATGGGAAGCTGAATCGATTGATCGGGAAA

ACCAACGAGAAATTCCATCAGATTGAGAAAGAATTCTCAGAAGTAGAA

GGGAGAATTCAGGACCTTGAGAAATATGTTGAGGACACAAAAATAGA

TCTCTGGTCATACAACGCAGAGCTTCTTGTTGCCCTGGAAAACCAAC

ATACAATTGATCTAACTGACTCAGAAATGAACAAACTGTTTGAAAAAA

CAAAGAAGCAACTGAGGGAAAATGCTGAGGATATGGGCAATGGTTGT

TTCAAAATATACCACAAATGTGACAATGCCTGCATAGGATCAATCAGA

AATGGAACTTATGACCACGATGTATACAGGGATGAAGCATTAAACAA

CCGGTTCCAGATCAAGGGAGTTGAGCTGAAGTCAGGGTACAAAGATT

GGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGC

TTTGTTGGGGTTCATCATGTGGGCCTGCCAAAAGGGCAACATTAGGT

GCAACATTTGCATTTGAGTGCATTAATTAAAAACACCCTTGTTTCTACT

GGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGT

TGAAGAAAACCCCGGGCCTATTTTGTAATATCCACAAAATGAAGGCAA

TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCA

CTGGGATAACATCGTCAAACTCACCACATGTCGTCAAAACTGCTACT

CAAGGGGAGGTCAACGTGACCGGTGTAATACCACTGACAACAACAC

CCACCAAATCTCATTTTGCAAATCTCAAAGGAACAGAAACCAGGGGG

AAACTATGCCCAAAATGCCTCAACTGCACAGATCTGGATGTAGCCTT

GGGCAGACCAAAATGCACAGGGAAAATACCCTCTGCAAGGGTTTCAA

TACTCCATGAAGTCAGACCTGTTACATCTGGGTGCTTTCCTATAATGC

ACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTCCGAGGATAC

GAACATGTCAGGTTATCAACTCACAACGTTATCAATGCAAAAGATGCA

CCAGGAAGACCCTACGAAATTGGAACCTCAGGGTCTTGCCCTAACAT

TACCAATGGAAACGGATTCTTCGCAACAATGGCTTGGGCCGTCCCAA

AAAACAAAACAGCAACAAATCCATTAAGAATAGAAGTACCATACATTT

GTACAGAAGGAGAAGACCAAATTACCGTTTGGGGGTTCCACTCTGAC

AACGAGACCCAAATGGCAAAGCTCTATGGGGACTCAAAGCCCCAGA

AGTTCACCTCATCTGCCAACGGAGTGACCACACATTACGTTTCACAG

ATTGGTGGCTTCCCAAATCAAACAGAAGACGGAGGACTACCACAAAG

TGGCAGAATTGTTGTTGATTACATGGTGCAGAAATCTGGAAAAACAG

GAACAATTACCTATCAAAGAGGTATTTTATTGCCTCAAAAAGTGTGGT

GCGCAAGTGGCAGGAGCAAGGTAATAAAAGGATCCTTGCCCTTAATT

GGAGAAGCAGATTGCCTCCATGAAAAATACGGTGGATTAAACAAAAG

CAAGCCTTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCC

CAATATGGGTGAAAACACCCTTGAAGCTGGCCAATGGAACCAAATAT

AGACCCCCTGCAAAACTATTAAAGGAAAGAGGTTTCTTCGGAGCCAT

TGCTGGTTTCTTAGAGGGAGGATGGGAAGGAATGATTGCAGGTTGG

CACGGATACACATCCCATGGGGCACATGGAGTAGCGGTGGCAGCTG

ACCTTAAGAGCACTCAAGAGGCCATAAACAAGATAACAAAAAATCTCA

ACTCTTTGAGTGAGCTGGAAGTAAAGAATCTTCAAAGACTAAGCGGT

GCCATGGATGAACTCCACAACGAAATACTAGAACTAGATGAGAAAGT

GGATGATCTCAGAGCTGACACAATAAGCTCACAAATAGAACTCGCAG

TCCTGCTTTCCAATGAAGGAATAATAAACAGTGAAGATGAACATCTCT

TGGCGCTTGAAAGAAAGCTGAAGAAAATGCTGGGCCCCTCTGCTGTA

GAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCAGAC

CTGCCTCGACAGAATAGCTGCTGGTACCTTTGATGCAGGAGAATTTT

CTCTCCCCACCTTTGATTCACTGAATATTACTGCTGCATCTTTAAATG

ACGACGGATTGGACAATCATACTATACTGCTTTACTACTCAACTGCTG

CCTCCAGTTTGGCTGTAACACTGATGATAGCTATCTTTGTTGTTTATA

TGGTCTCCAGAGACAATGTTTCTTGCTCCATTTGTCTATAAGGAAAGT

TAAGCCCTGTATTTTCCTTTATTGTAGTGCTTGTTTGCTTGTTGTCATT

ACAAAGAAACGTTATTGAAAAATTGATGACTCGAGCTGGTACTGCATG

CACGCAATGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCCGAGTCTC

CCCCGACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGCCCCAC

TCACCACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCAATGC

AGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGT

GATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCC

CAGGGTTGGTCAATTTCGTGCCAGCCACACCCTGGAGCTAGCAAAAA

AAA

Pan-CoV-Flu
CTCGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGT
410

Vaccine #22
CATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGG

CoV-Wuhan
TAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGAC

Spike(2 proline
GTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCA

substitution)-
TTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAG

Influenza (HA)
TACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATG

ACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGG

GACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACC

ATGGTCGAGGTGAGCCCCACGTTCTGCTTCACTCTCCCCATCTCCCC

CCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTTTGT

GCAGCGATGGGGGCGGGGGGGGGGGGGGGGCGCGCGCCAGGCG

GGGCGGGGCGGGGCGAGGGGCGGGGCGGGGCGAGGCGGAGAGG

TGCGGCGGCAGCCAATCAGAGCGGCGCGCTCCGAAAGTTTCCTTTT

ATGGCGAGGCGGCGGCGGCGGCGGCCCTATAAAAAGCGAAGCGCG

CGGCGGGCGGGAGTCGCTGCGCGCTGCCTTCGCCCCGTGCCCCGC

TCCGCCGCCGCCTCGCGCCGCCCGCCCCGGCTCTGACTGACCGCG

TTACTCCCACAGGTGAGCGGGCGGGACGGCCCTTCTCCTCCGGGCT

GTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGC

GTGAAAGCCTTGAGGGGCTCCGGGAGGGCCCTTTGTGCGGGGGGA

GCGGCTCGGGGGGTGCGTGCGTGTGTGTGTGCGTGGGGAGCGCCG

CGTGCGGCTCCGCGCTGCCCGGCGGCTGTGAGCGCTGCGGGCGCG

GCGCGGGGCTTTGTGCGCTCCGCAGTGTGCGCGAGGGGAGCGCGG

CCGGGGGCGGTGCCCCGCGGTGCGGGGGGGGCTGCGAGGGGAAC

AAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGGGTGAGCAGGGGGT

GTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTGCACCCCCCTCCC

CGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGGGCTCCGTACG

GGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGGTGGCGGCA

GGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCCGGGGAG

GGCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGCGGCTG

TCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCGTGC

GAGAGGGCGCAGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCGA

AATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGA

AGCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTC

GTGCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGG

CTGTCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGG

CGGGGTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGC

TAACCATGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGT

GCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAGAATTGGAGAATAA

ACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGAACCCGCCACCA

TGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCGT

GAACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAGC

TTCACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCG

TGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTG

ACCTGGTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAGA

GGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGC

CAGCACCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACC

ACCCTGGACAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCA

CCAACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCC

CTTCCTGGGCGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGA

GCGAGTTCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTA

CGTGAGCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAAC

TTCAAGAACCTGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTT

CAAGATCTACAGCAAGCACACCCCCATCAACCTGGTGAGGGACCTG

CCCCAGGGCTTCAGCGCCCTGGAGCCCCTGGTGGACCTGCCCATCG

GCATCAACATCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAG

CTACCTGACCCCCGGCGACAGCAGCAGCGGCTGGACCGCCGGCGC

CGCCGCCTACTACGTGGGCTACCTGCAGCCCAGGACCTTCCTGCTG

AAGTACAACGAGAACGGCACCATCACCGACGCCGTGGACTGCGCCC

TGGACCCCCTGAGCGAGACCAAGTGCACCCTGAAGAGCTTCACCGT

GGAGAAGGGCATCTACCAGACCAGCAACTTCAGGGTGCAGCCCACC

GAGAGCATCGTGAGGTTCCCCAACATCACCAACCTGTGCCCCTTCG

GCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGTACGCCTGGAA

CAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTAC

AACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTGAGCCCCA

CCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTC

GTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCAGACC

GGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCACCG

GCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGGG

CGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAG

CACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTG

CAGAGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCT

ACAGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCAC

CGTGTGCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGC

GTGAACTTCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCG

AGAGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACAT

CGCCGACACCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGAT

CCTGGACATCACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACC

CCCGGCACCAACACCAGCAACCAGGTGGCCGTGCTGTACCAGGACG

TGAACTGCACCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGAC

CCCCACCTGGAGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACC

AGGGCCGGCTGCCTGATCGGCGCCGAGCACGTGAACAACAGCTAC

GAGTGCGACATCCCCATCGGCGCCGGCATCTGCGCCAGCTACCAGA

CCCAGACCAACAGCCCCAGGAGGGCCAGGAGCGTGGCCAGCCAGA

GCATCATCGCCTACACCATGAGCCTGGGCGCCGAGAACAGCGTGGC

CTACAGCAACAACAGCATCGCCATCCCCACCAACTTCACCATCAGCG

TGACCACCGAGATCCTGCCCGTGAGCATGACCAAGACCAGCGTGGA

CTGCACCATGTACATCTGCGGCGACAGCACCGAGTGCAGCAACCTG

CTGCTGCAGTACGGCAGCTTCTGCACCCAGCTGAACAGGGCCCTGA

CCGGCATCGCCGTGGAGCAGGACAAGAACACCCAGGAGGTGTTCGC

CCAGGTGAAGCAGATCTACAAGACCCCCCCCATCAAGGACTTCGGC

GGCTTCAACTTCAGCCAGATCCTGCCCGACCCCAGCAAGCCCAGCA

AGAGGAGCTTCATCGAGGACCTGCTGTTCAACAAGGTGACCCTGGC

CGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCTGGGCGACATC

GCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACGGCCTGACCG

TGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAGTACACCAG

CGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTTCGGCGC

CGGCGCCGCCCTGCAGATCCCCTTCGCCATGCAGATGGCCTACAGG

TTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACCAGA

AGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGGA

CAGCCTGAGCAGCACCGCCAGCGCCCTGGGCAAGCTGCAGGACGT

GGTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTG

AGCAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGA

GCAGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGA

TCACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCT

GATCAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCAC

CAAGATGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTC

TGCGGCAAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCC

ACGGCGTGGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAA

GAACTTCACCACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCAC

TTCCCCAGGGAGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCG

TGACCCAGAGGAACTTCTACGAGCCCCAGATCATCACCACCGACAAC

ACCTTCGTGAGCGGCAACTGCGACGTGGTGATCGGCATCGTGAACA

ACACCGTGTACGACCCCCTGCAGCCCGAGCTGGACAGCTTCAAGGA

GGAGCTGGACAAGTACTTCAAGAACCACACCAGCCCCGACGTGGAC

CTGGGCGACATCAGCGGCATCAACGCCAGCGTGGTGAACATCCAGA

AGGAGATCGACAGGCTGAACGAGGTGGCCAAGAACCTGAACGAGAG

CCTGATCGACCTGCAGGAGCTGGGCAAGTACGAGCAGTACATCAAG

TGGCCCTGGTACATCTGGCTGGGCTTCATCGCCGGCCTGATCGCCA

TCGTGATGGTGACCATCATGCTGTGCTGCATGACCAGCTGCTGCAGC

TGCCTGAAGGGCTGCTGCAGCTGCGGCAGCTGCTGCAAGTTCGACG

AGGACGACAGCGAGCCCGTGCTGAAGGGCGTGAAGCTGCACTACAC

CGGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGAT

GTTGAAGAAAACCCCGGGCCTATGAAGGCAATACTAGTAGTTCTGCT

ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCA

TGCGAACAACTCAACAGACACTGTAGACACAGTACTAGAAAAGAATG

TAACAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGA

AACTATGCAAACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGT

AACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCTC

CACAGCAAGCTCATGGTCCTACATTGTGGAAACATCTAGTTCAGACA

ATGGAACGTGTTACCCAGGAGATTTCATCGATTATGAGGAGCTAAGA

GAGCAATTGAGCTCAGTGTCATCATTTGAAAGGTTTGAGATATTCCCC

AAGACAAGTTCATGGCCCAATCATGACTCGAACAAAGGTGTAACGAC

AGCATGTCCTCATGCTGGAGCAAAAAGCTTCTACAAAAATTTAATATG

GCTAGTTAAAAAAGGAAATTCATACCCAAAGCTCAGCAAATCCTACAT

TAATGATAAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTCACCATC

CACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCA

TATGTTTTTGTGGGGACATCAAGATACAGCAAGAAGTTCAAGCCGGA

AATAGCAATAAGGCCCAAAGTGAGGGATCAAGAAGGGAGAATGAACT

ATTACTGGACACTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCA

ACTGGAAATCTAGTGGTACCGAGATATGCATTCGCAATGGAAAGAAA

TGCTGGATCTGGTATTATCATTTCAGATACACCAGTCCACGATTGCAA

TACAACTTGTCAGACACCCAAGGGTGCTATAAACACCAGCCTCCCAT

TTCAGAACATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAA

AAAGCACAAAATTGAGACTGGCCACAGGATTGAGGAATGTCCCGTCC

ATTCAATCTAGAGGCCTATTTGGGGCCATTGCCGGTTTCATTGAAGG

GGGGTGGACAGGGATGGTAGATGGATGGTACGGTTATCACCATCAA

AATGAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACACAGA

ATGCCATTGACGAGATTACTAACAAAGTAAACTCTGTTATTGAAAAAA

TGAATACACAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAA

AAAAGAATAGAGAATTTAAATAAAAAAGTTGATGATGGTTTCCTGGAC

ATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGA

ACTTTGGACTACCACGATTCAAAGGTGAAGAACTTATATGAAAAGGTA

AGAAGCCAGTTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTT

TGAATTTTACCACAAATGTGATAACACGTGCATGGAAAGTGTCAAAAA

TGGGACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACA

GAGAAGAAATAGATGGGGTAAAGCTGGAATCAACAAGGATTTACCAG

ATTTTGGCGATCTATTCAACCGTCGCCAGTTCATTGGTACTGGTAGTC

TCCCTGGGGGCAATCAGTTTCTGGATGTGCTCTAATGGGTCTCTACA

GTGTAGAATATGTATTTAAGGAAGCGGAGCCACGAACTTCTCTCTGTT

AAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTAGCAAAAGC

AGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTTGAGCTACA

TTCTATGTCTGGTTTTCGCTCAAAAACTTCCTGGAAATGACAATAGCA

CTGCAACGCTGTGCCTTGGGCACCATGCAGTACCAAACGGAACGAT

AGTGAAAACAATCACGAATGACCAAATTGAAGTTACTAATGCTACTGA

GCTGGTTCAGAATTCCTCAATAGGTGAAATATGCGACAGTCCTCATC

AGATCCTTGATGGAGAAAACTGCACACTAATAGATGCTCTATTGGGA

GACCCTCAGTGTGATGGCTTTCAAAATAAGAAATGGGACCTTTTTGTT

GAACGAAGCAAAGCCTACAGCAACTGTTACCCTTATGATGTGCCGGA

TTATGCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGT

TTAACAATGAAAGCTTCAATTGGGCTGGAGTCACTCAAAACGGAACA

AGTTCTGCTTGCATAAGGGGATCTAATAGTAGTTTCTTTAGTAGATTA

AATTGGTTGACCCACTTAAACTTCAAGTACCCAGCATTGAACGTGACT

ATGCCAAACAATGAACAATTTGACAAATTGTACATTTGGGGGGTTCAC

CACCCGGGTACGGACAAGGACCAAATCTTCCTGTATGCTCAATCATC

AGGAAGAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCC

CGAATATCGGATCTAGACCCAGAATAAGGAATATCCCTAGCAGAATA

AGCATCTATTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAAC

AGCACAGGGAATCTAATTGCTCCTAGGGGTTACTTCAAAATACGAAG

TGGGAAAAGCTCAATAATGAGATCAGATGCACCCATTGGCAAATGCA

AGTCTGAATGCATCACTCCAAATGGAAGCATTCCCAATGACAAACCAT

TCCAAAATGTAAACAGGATCACATACGGGGCCTGTCCCAGATATGTT

AAGCAAAGCACTCTGAAATTGGCAACAGGAATGCGAAATGTACCAGA

GAAACAAACTAGAGGCATATTTGGCGCAATAGCGGGTTTCATAGAAA

ATGGTTGGGAGGGAATGGTGGATGGTTGGTACGGTTTCAGGCATCA

AAATTCTGAGGGAAGAGGACAAGCAGCAGATCTCAAAAGCACTCAAG

CAGCAATCGATCAAATCAATGGGAAGCTGAATCGATTGATCGGGAAA

ACCAACGAGAAATTCCATCAGATTGAGAAAGAATTCTCAGAAGTAGAA

GGGAGAATTCAGGACCTTGAGAAATATGTTGAGGACACAAAAATAGA

TCTCTGGTCATACAACGCAGAGCTTCTTGTTGCCCTGGAAAACCAAC

ATACAATTGATCTAACTGACTCAGAAATGAACAAACTGTTTGAAAAAA

CAAAGAAGCAACTGAGGGAAAATGCTGAGGATATGGGCAATGGTTGT

TTCAAAATATACCACAAATGTGACAATGCCTGCATAGGATCAATCAGA

AATGGAACTTATGACCACGATGTATACAGGGATGAAGCATTAAACAA

CCGGTTCCAGATCAAGGGAGTTGAGCTGAAGTCAGGGTACAAAGATT

GGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGC

TTTGTTGGGGTTCATCATGTGGGCCTGCCAAAAGGGCAACATTAGGT

GCAACATTTGCATTTGAGTGCATTAATTAAAAACACCCTTGTTTCTACT

GGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGT

TGAAGAAAACCCCGGGCCTATTTTCTAATATCCACAAAATGAAGGCAA

TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCA

CTGGGATAACATCGTCAAACTCACCACATGTCGTCAAAACTGCTACT

CAAGGGGAGGTCAACGTGACCGGTGTAATACCACTGACAACAACAC

CCACCAAATCTCATTTTGCAAATCTCAAAGGAACAGAAACCAGGGGG

AAACTATGCCCAAAATGCCTCAACTGCACAGATCTGGATGTAGCCTT

GGGCAGACCAAAATGCACAGGGAAAATACCCTCTGCAAGGGTTTCAA

TACTCCATGAAGTCAGACCTGTTACATCTGGGTGCTTTCCTATAATGC

ACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTCCGAGGATAC

GAACATGTCAGGTTATCAACTCACAACGTTATCAATGCAAAAGATGCA

CCAGGAAGACCCTACGAAATTGGAACCTCAGGGTCTTGCCCTAACAT

TACCAATGGAAACGGATTCTTCGCAACAATGGCTTGGGCCGTCCCAA

AAAACAAAACAGCAACAAATCCATTAACAATAGAAGTACCATACATTT

GTACAGAAGGAGAAGACCAAATTACCGTTTGGGGGTTCCACTCTGAC

AACGAGACCCAAATGGCAAAGCTCTATGGGGACTCAAAGCCCCAGA

AGTTCACCTCATCTGCCAACGGAGTGACCACACATTACGTTTCACAG

ATTGGTGGCTTCCCAAATCAAACAGAAGACGGAGGACTACCACAAAG

TGGCAGAATTGTTGTTGATTACATGGTGCAGAAATCTGGAAAAACAG

GAACAATTACCTATCAAAGAGGTATTTTATTGCCTCAAAAAGTGTGGT

GCGCAAGTGGCAGGAGCAAGGTAATAAAAGGATCCTTGCCCTTAATT

GGAGAAGCAGATTGCCTCCATGAAAAATACGGTGGATTAAACAAAAG

CAAGCCTTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCC

CAATATGGGTGAAAACACCCTTGAAGCTGGCCAATGGAACCAAATAT

AGACCCCCTGCAAAACTATTAAAGGAAAGAGGTTTCTTCGGAGCCAT

TGCTGGTTTCTTAGAGGGAGGATGGGAAGGAATGATTGCAGGTTGG

CACGGATACACATCCCATGGGGCACATGGAGTAGCGGTGGCAGCTG

ACCTTAAGAGCACTCAAGAGGCCATAAACAAGATAACAAAAAATCTCA

ACTCTTTGAGTGAGCTGGAAGTAAAGAATCTTCAAAGACTAAGCGGT

GCCATGGATGAACTCCACAACGAAATACTAGAACTAGATGAGAAAGT

GGATGATCTCAGAGCTGACACAATAAGCTCACAAATAGAACTCGCAG

TCCTGCTTTCCAATGAAGGAATAATAAACAGTGAAGATGAACATCTCT

TGGCGCTTGAAAGAAAGCTGAAGAAAATGCTGGGCCCCTCTGCTGTA

GAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCAGAC

CTGCCTCGACAGAATAGCTGCTGGTACCTTTGATGCAGGAGAATTTT

CTCTCCCCACCTTTGATTCACTGAATATTACTGCTGCATCTTTAAATG

ACGACGGATTGGACAATCATACTATACTGCTTTACTACTCAACTGCTG

CCTCCAGTTTGGCTGTAACACTGATGATAGCTATCTTTGTTGTTTATA

TGGTCTCCAGAGACAATGTTTCTTGCTCCATTTGTCTATAAGGAAAGT

TAAGCCCTGTATTTTCCTTTATTGTAGTGCTTGTTTGCTTGTTGTCATT

ACAAAGAAACGTTATTGAAAAATTGATGACTCGAGCTGGTACTGCATG

CACGCAATGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCCGAGTCTC

CCCCGACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGCCCCAC

TCACCACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCAATGC

AGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGT

GATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCC

CAGGGTTGGTCAATTTCGTGCCAGCCACACCCTGGAGCTAGCAAAAA

AAA

Pan-CoV-Flu
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCG
411

Vaccine #23
TGAACCTGATCACCAGGACCCAGAGCTACACCAACAGCTTCACCAGG

CoV- Omicron
GGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCTGCACA

BA.2
GCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTGGTTC

Spike(HexaPra)-
CACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGACA

Influenza (HA)
ACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGAC

AGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGG

TGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGAC

GTGTACTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCA

GGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCA

GCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAAC

CTGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTA

CAGCAAGCACACCCCCATCAACCTGGGCAGGGACCTGCCCCAGGGC

TTCAGCGCCCTGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACA

TCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGAC

CCCCGGCGACAGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTA

CTACGTGGGCTACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAAC

GAGAACGGCACCATCACCGACGCCGTGGACTGCGCCCTGGACCCC

CTGAGCGAGACCAAGTGCACCCTGAAGAGCTTCACCGTGGAGAAGG

GCATCTACCAGACCAGCAACTTCAGGGTGCAGCCCACCGAGAGCAT

CGTGAGGTTCCCCAACATCACCAACCTGTGCCCCTTCGACGAGGTGT

TCAACGCCACCAGGTTCGCCAGCGTGTACGCCTGGAACAGGAAGAG

GATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTACAACTTCGCC

CCCTTCTTCGCCTTCAAGTGCTACGGCGTGAGCCCCACCAAGCTGAA

CGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTCGTGATCAGG

GGCAACGAGGTGAGCCAGATCGCCCCCGGCCAGACCGGCAACATC

GCCGACTACAACTACAAGCTGCCCGACGACTTCACCGGCTGCGTGA

TCGCCTGGAACAGCAACAAGCTGGACAGCAAGGTGGGCGGCAACTA

CAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGAAGCCCTTCG

AGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAACAAGCCCTG

CAACGGCGTGGCCGGCTTCAACTGCTACTTCCCCCTGAGGAGCTAC

GGCTTCAGGCCCACCTACGGCGTGGGCCACCAGCCCTACAGGGTG

GTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGTGCG

GCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACTT

CAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAAC

AAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACA

CCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACAT

CACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACC

AACACCAGCAACCAGGTGGCCGTGCTGTACCAGGGCGTGAACTGCA

CCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTG

GAGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGG

CTGCCTGATCGGCGCCGAGTACGTGAACAACAGCTACGAGTGCGAC

ATCCCCATCGGCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCA

AGAGCCACCAGCAGGCCCAGAGCGTGGCCAGCCAGAGCATCATCG

CCTACACCATGAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAA

CAACAGCATCGCCATCCCCACCAACTTCACCATCAGCGTGACCACCG

AGATCCTGCCCGTGAGCATGACCAAGACCAGCGTGGACTGCACCAT

GTACATCTGCGGCGACAGCACCGAGTGCAGCAACCTGCTGCTGCAG

TACGGCAGCTTCTGCACCCAGCTGAAGAGGGCCCTGACCGGCATCG

CCGTGGAGCAGGACAAGAACACCCAGGAGGTGTTCGCCCAGGTGAA

GCAGATCTACAAGACCCCCCCCATCAAGTACTTCGGCGGCTTCAACT

TCAGCCAGATCCTGCCCGACCCCAGCAAGCCCAGCAAGAGGAGCCC

CATCGAGGACCTGCTGTTCAACAAGGTGACCCTGGCCGACGCCGGC

TTCATCAAGCAGTACGGCGACTGCCTGGGCGACATCGCCGCCAGGG

ACCTGATCTGCGCCCAGAAGTTCAACGGCCTGACCGTGCTGCCCCC

CCTGCTGACCGACGAGATGATCGCCCAGTACACCAGCGCCCTGCTG

GCCGGCACCATCACCAGCGGCTGGACCTTCGGCGCCGGCCCCGCC

CTGCAGATCCCCTTCCCCATGCAGATGGCCTACAGGTTCAACGGCAT

CGGCGTGACCCAGAACGTGCTGTACGAGAACCAGAAGCTGATCGCC

AACCAGTTCAACAGCGCCATCGGCAAGATCCAGGACAGCCTGAGCA

GCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGTGGTGAACCACAA

CGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAGCAGCAAGTTC

GGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAGGCTGGACC

CCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCGGCAGGC

TGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGGGCCGC

CGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAGCGA

GTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGGG

CTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTG

TTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCA

CCGCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGA

GGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGG

AACTTCTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAG

CGGCAACTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTAC

GACCCCCTGCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACA

AGTACTTCAAGAACCACACCAGCCCCGACGTGGACCTGGGCGACAT

CAGCGGCATCAACGCCAGCGTGGTGAACATCCAGAAGGAGATCGAC

AGGCTGAACGAGGTGGCCAAGAACCTGAACGAGAGCCTGATCGACC

TGCAGGAGCTGGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGTA

CATCTGGCTGGGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTG

ACCATCATGCTGTGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGG

GCTGCTGCAGCTGCGGCAGCTGCTGCAAGTTCGACGAGGACGACAG

CGAGCCCGTGCTGAAGGGCGTGAAGCTGCACTACACCGGAAGCGG

AGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAAA

ACCCCGGGCCTATGAAGGCAATACTAGTAGTTCTGCTATATACATTTG

CAACCGCAAATGCAGACACATTATGTATAGGTTATCATGCGAACAACT

CAACAGACACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACA

CACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAA

CTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGCTGG

CTGGATCCTGGGAAATCCAGAGTGTGAATCACTCTCCACAGCAAGCT

CATGGTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTT

ACCCAGGAGATTTCATCGATTATGAGGAGCTAAGAGAGCAATTGAGC

TCAGTGTCATCATTTGAAAGGTTTGAGATATTCCCCAAGACAAGTTCA

TGGCCCAATCATGACTCGAACAAAGGTGTAACGACAGCATGTCCTCA

TGCTGGAGCAAAAAGCTTCTACAAAAATTTAATATGGCTAGTTAAAAA

AGGAAATTCATACCCAAAGCTCAGGAAATCCTACATTAATGATAAAGG

GAAAGAAGTCCTCGTGCTATGGGGCATTCACCATCCACCTACTAGTG

CTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTTTTGTGG

GGACATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGG

CCCAAAGTGAGGGATCAAGAAGGGAGAATGAACTATTACTGGACACT

AGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAG

TGGTACCGAGATATGCATTCGCAATGGAAAGAAATGCTGGATCTGGT

ATTATCATTTCAGATACACCAGTCCACGATTGCAATACAACTTGTCAG

ACACCCAAGGGTGCTATAAACACCAGCCTCCCATTTCAGAACATACA

TCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGCACAAAATT

GAGACTGGCCACAGGATTGAGGAATGTCCCGTCCATTCAATCTAGAG

GCCTATTTGGGGCCATTGCCGGTTTCATTGAAGGGGGGTGGACAGG

GATGGTAGATGGATGGTACGGTTATCACCATCAAAATGAGCAGGGGT

CAGGATATGCAGCCGACCTGAAGAGCACACAGAATGCCATTGACGA

GATTACTAACAAAGTAAACTCTGTTATTGAAAAAATGAATACACAGTTC

ACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGAA

TTTAAATAAAAAAGTTGATGATGGTTTCCTGGACATTTGGACTTACAAT

GCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCA

CGATTCAAAGGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGTTAAA

AAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACCACAA

ATGTGATAACACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACT

ACCCAAAATACTGAGAGGAAGGAAAATTAAACAGAGAAGAAATAGAT

GGGGTAAAGCTGGAATCAACAAGGATTTACCAGATTTTGGCGATCTA

TTCAACCGTCGCCAGTTCATTGGTACTGGTAGTCTCCCTGGGGGCAA

TCAGTTTCTGGATGTGCTCTAATGGGTCTCTACAGTGTAGAATATGTA

TTTAAGGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGA

GATGTTGAAGAAAACCCCGGGCCTAGCAAAAGCAGGGGATAATTCTA

TTAACCATGAAGACTATCATTGCTTTGAGCTACATTCTATGTCTGGTTT

TCGCTCAAAAACTTCCTGGAAATGACAATAGCACTGCAACGCTGTGC

CTTGGGCACCATGCAGTACCAAACGGAACGATAGTGAAAACAATCAC

GAATGACCAAATTGAAGTTACTAATGCTACTGAGCTGGTTCAGAATTC

CTCAATAGGTGAAATATGCGACAGTCCTCATCAGATCCTTGATGGAG

AAAACTGCACACTAATAGATGCTCTATTGGGAGACCCTCAGTGTGAT

GGCTTTCAAAATAAGAAATGGGACCTTTTTGTTGAACGAAGCAAAGC

CTACAGCAACTGTTACCCTTATGATGTGCCGGATTATGCCTCCCTTAG

GTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGAAAGCT

TCAATTGGGCTGGAGTCACTCAAAACGGAACAAGTTCTGCTTGCATA

AGGGGATCTAATAGTAGTTTCTTTAGTAGATTAAATTGGTTGACCCAC

TTAAACTTCAAGTACCCAGCATTGAACGTGACTATGCCAAACAATGAA

CAATTTGACAAATTGTACATTTGGGGGGTTCACCACCCGGGTACGGA

CAAGGACCAAATCTTCCTGTATGCTCAATCATCAGGAAGAATCACAGT

ATCTACCAAAAGAAGCCAACAAGCTGTAATCCCGAATATCGGATCTA

GACCCAGAATAAGGAATATCCCTAGCAGAATAAGCATCTATTGGACA

ATAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAGGGAATCTA

ATTGCTCCTAGGGGTTACTTCAAAATACGAAGTGGGAAAAGCTCAAT

AATGAGATCAGATGCACCCATTGGCAAATGCAAGTCTGAATGCATCA

CTCCAAATGGAAGCATTCCCAATGACAAACCATTCCAAAATGTAAACA

GGATCACATACGGGGCCTGTCCCAGATATGTTAAGCAAAGCACTCTG

AAATTGGCAACAGGAATGCGAAATGTACCAGAGAAACAAACTAGAGG

CATATTTGGCGCAATAGCGGGTTTCATAGAAAATGGTTGGGAGGGAA

TGGTGGATGGTTGGTACGGTTTCAGGCATCAAAATTCTGAGGGAAGA

GGACAAGCAGCAGATCTCAAAAGCACTCAAGCAGCAATCGATCAAAT

CAATGGGAAGCTGAATCGATTGATCGGGAAAACCAACGAGAAATTCC

ATCAGATTGAGAAAGAATTCTCAGAAGTAGAAGGGAGAATTCAGGAC

CTTGAGAAATATGTTGAGGACACAAAAATAGATCTCTGGTCATACAAC

GCAGAGCTTCTTGTTGCCCTGGAAAACCAACATACAATTGATCTAACT

GACTCAGAAATGAACAAACTGTTTGAAAAAACAAAGAAGCAACTGAG

GGAAAATGCTGAGGATATGGGCAATGGTTGTTTCAAAATATACCACA

AATGTGACAATGCCTGCATAGGATCAATCAGAAATGGAACTTATGAC

CACGATGTATACAGGGATGAAGCATTAAACAACCGGTTCCAGATCAA

GGGAGTTGAGCTGAAGTCAGGGTACAAAGATTGGATCCTATGGATTT

CCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCAT

CATGTGGGCCTGCCAAAAGGGCAACATTAGGTGCAACATTTGCATTT

GAGTGCATTAATTAAAAACACCCTTGTTTCTACTGGAAGCGGAGCCA

CGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCC

GGGCCTATTTTCTAATATCCACAAAATGAAGGCAATAATTGTACTACT

CATGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACAT

CGTCAAACTCACCACATGTCGTCAAAACTGCTACTCAAGGGGAGGTC

AACGTGACCGGTGTAATACCACTGACAACAACACCCACCAAATCTCA

TTTTGCAAATCTCAAAGGAACAGAAACCAGGGGGAAACTATGCCCAA

AATGCCTCAACTGCACAGATCTGGATGTAGCCTTGGGCAGACCAAAA

TGCACAGGGAAAATACCCTCTGCAAGGGTTTCAATACTCCATGAAGT

CAGACCTGTTACATCTGGGTGCTTTCCTATAATGCACGATAGAACAAA

AATTAGACAGCTGCCTAACCTTCTCCGAGGATACGAACATGTCAGGT

TATCAACTCACAACGTTATCAATGCAAAAGATGCACCAGGAAGACCC

TACGAAATTGGAACCTCAGGGTCTTGCCCTAACATTACCAATGGAAA

CGGATTCTTCGCAACAATGGCTTGGGCCGTCCCAAAAAACAAAACAG

CAACAAATCCATTAACAATAGAAGTACCATACATTTGTACAGAAGGAG

AAGACCAAATTACCGTTTGGGGGTTCCACTCTGACAACGAGACCCAA

ATGGCAAAGCTCTATGGGGACTCAAAGCCCCAGAAGTTCACCTCATC

TGCCAACGGAGTGACCACACATTACGTTTCACAGATTGGTGGCTTCC

CAAATCAAACAGAAGACGGAGGACTACCACAAAGTGGCAGAATTGTT

GTTGATTACATGGTGCAGAAATCTGGAAAAACAGGAACAATTACCTAT

CAAAGAGGTATTTTATTGCCTCAAAAAGTGTGGTGCGCAAGTGGCAG

GAGCAAGGTAATAAAAGGATCCTTGCCCTTAATTGGAGAAGCAGATT

GCCTCCATGAAAAATACGGTGGATTAAACAAAAGCAAGCCTTACTAC

ACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGGTGAA

AACACCCTTGAAGCTGGCCAATGGAACCAAATATAGACCCCCTGCAA

AACTATTAAAGGAAAGAGGTTTCTTCGGAGCCATTGCTGGTTTCTTAG

AGGGAGGATGGGAAGGAATGATTGCAGGTTGGCACGGATACACATC

CCATGGGGCACATGGAGTAGCGGTGGCAGCTGACCTTAAGAGCACT

CAAGAGGCCATAAACAAGATAACAAAAAATCTCAACTCTTTGAGTGAG

CTGGAAGTAAAGAATCTTCAAAGACTAAGCGGTGCCATGGATGAACT

CCACAACGAAATACTAGAACTAGATGAGAAAGTGGATGATCTCAGAG

CTGACACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTTCCAATG

AAGGAATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGA

AAGCTGAAGAAAATGCTGGGCCCCTCTGCTGTAGAGATAGGGAATG

GATGCTTTGAAACCAAACACAAGTGCAACCAGACCTGCCTCGACAGA

ATAGCTGCTGGTACCTTTGATGCAGGAGAATTTTCTCTCCCCACCTTT

GATTCACTGAATATTACTGCTGCATCTTTAAATGACGACGGATTGGAC

AATCATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCT

GTAACACTGATGATAGCTATCTTTGTTGTTTATATGGTCTCCAGAGAC

AATGTTTCTTGCTCCATTTGTCTATAAGGAAAGTTAAGCCCTGTATTTT

CCTTTATTGTAGTGCTTGTTTGCTTGTTGTCATTACAAAGAAACGTTAT

TGAAAAATGGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCA

GGAGATGTTGAAGAAAACCCCGGGCCTTTCGGAGCTATTGCTGGTTT

CTTGGAAGGAGGATGGGAAGGAATGATTGCAGGTTGGCACGGATAC

ACATCTCATGGAGCACATGGAGTAGCAGTGGCAGCAGACCTTAAGA

GTACCCATGATGA

Pan-CoV-Flu
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCG
412

Vaccine #24
TGAACCTGATCACCAGGACCCAGAGCTACACCAACAGCTTCACCAGG

CoV-Omicron
GGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCTGCACA

BA.2 Spike(2
GCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTGGTTC

proline
CACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGACA

substitution)-
ACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

Influenza (HA)
GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGAC

AGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGG

TGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGAC

GTGTACTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCA

GGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCA

GCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAAC

CTGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTA

CAGCAAGCACACCCCCATCAACCTGGGCAGGGACCTGCCCCAGGGC

TTCAGCGCCCTGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACA

TCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGAC

CCCCGGCGACAGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTA

CTACGTGGGCTACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAAC

GAGAACGGCACCATCACCGACGCCGTGGACTGCGCCCTGGACCCC

CTGAGCGAGACCAAGTGCACCCTGAAGAGCTTCACCGTGGAGAAGG

GCATCTACCAGACCAGCAACTTCAGGGTGCAGCCCACCGAGAGCAT

CGTGAGGTTCCCCAACATCACCAACCTGTGCCCCTTCGACGAGGTGT

TCAACGCCACCAGGTTCGCCAGCGTGTACGCCTGGAACAGGAAGAG

GATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTACAACTTCGCC

CCCTTCTTCGCCTTCAAGTGCTACGGCGTGAGCCCCACCAAGCTGAA

CGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTCGTGATCAGG

GGCAACGAGGTGAGCCAGATCGCCCCCGGCCAGACCGGCAACATC

GCCGACTACAACTACAAGCTGCCCGACGACTTCACCGGCTGCGTGA

TCGCCTGGAACAGCAACAAGCTGGACAGCAAGGTGGGCGGCAACTA

CAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGAAGCCCTTCG

AGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAACAAGCCCTG

CAACGGCGTGGCCGGCTTCAACTGCTACTTCCCCCTGAGGAGCTAC

GGCTTCAGGCCCACCTACGGCGTGGGCCACCAGCCCTACAGGGTG

GTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGTGCG

GCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACTT

CAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAAC

AAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACA

CCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACAT

CACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACC

AACACCAGCAACCAGGTGGCCGTGCTGTACCAGGGCGTGAACTGCA

CCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTG

GAGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGG

CTGCCTGATCGGCGCCGAGTACGTGAACAACAGCTACGAGTGCGAC

ATCCCCATCGGCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCA

AGAGCCACCAGCAGGCCCAGAGCGTGGCCAGCCAGAGCATCATCG

CCTACACCATGAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAA

CAACAGCATCGCCATCCCCACCAACTTCACCATCAGCGTGACCACCG

AGATCCTGCCCGTGAGCATGACCAAGACCAGCGTGGACTGCACCAT

GTACATCTGCGGCGACAGCACCGAGTGCAGCAACCTGCTGCTGCAG

TACGGCAGCTTCTGCACCCAGCTGAAGAGGGCCCTGACCGGCATCG

CCGTGGAGCAGGACAAGAACACCCAGGAGGTGTTCGCCCAGGTGAA

GCAGATCTACAAGACCCCCCCCATCAAGTACTTCGGCGGCTTCAACT

TCAGCCAGATCCTGCCCGACCCCAGCAAGCCCAGCAAGAGGAGCTT

CATCGAGGACCTGCTGTTCAACAAGGTGACCCTGGCCGACGCCGGC

TTCATCAAGCAGTACGGCGACTGCCTGGGCGACATCGCCGCCAGGG

ACCTGATCTGCGCCCAGAAGTTCAACGGCCTGACCGTGCTGCCCCC

CCTGCTGACCGACGAGATGATCGCCCAGTACACCAGCGCCCTGCTG

GCCGGCACCATCACCAGCGGCTGGACCTTCGGCGCCGGCGCCGCC

CTGCAGATCCCCTTCGCCATGCAGATGGCCTACAGGTTCAACGGCAT

CGGCGTGACCCAGAACGTGCTGTACGAGAACCAGAAGCTGATCGCC

AACCAGTTCAACAGCGCCATCGGCAAGATCCAGGACAGCCTGAGCA

GCACCGCCAGCGCCCTGGGCAAGCTGCAGGACGTGGTGAACCACA

ACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAGCAGCAAGTT

CGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAGGCTGGAC

CCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCGGCAGG

CTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGGGCCG

CCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAGCG

AGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGG

GCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGT

GTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACC

ACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGG

AGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAG

GAACTTCTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGA

GCGGCAACTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTA

CGACCCCCTGCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGAC

AAGTACTTCAAGAACCACACCAGCCCCGACGTGGACCTGGGCGACA

TCAGCGGCATCAACGCCAGCGTGGTGAACATCCAGAAGGAGATCGA

CAGGCTGAACGAGGTGGCCAAGAACCTGAACGAGAGCCTGATCGAC

CTGCAGGAGCTGGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGT

ACATCTGGCTGGGCTTCATCGCCGGCCTGATCGCCATCGTGATGGT

GACCATCATGCTGTGCTGCATGACCAGCTGCTGCAGCTGCCTGAAG

GGCTGCTGCAGCTGCGGCAGCTGCTGCAAGTTCGACGAGGACGACA

GCGAGCCCGTGCTGAAGGGCGTGAAGCTGCACTACACCGGAAGCG

GAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAA

AACCCCGGGCCTATGAAGGCAATACTAGTAGTTCTGCTATATACATTT

GCAACCGCAAATGCAGACACATTATGTATAGGTTATCATGCGAACAA

CTCAACAGACACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAA

CACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGC

AAACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGC

TGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCTCCACAGCAA

GCTCATGGTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACG

TGTTACCCAGGAGATTTCATCGATTATGAGGAGCTAAGAGAGCAATT

GAGCTCAGTGTCATCATTTGAAAGGTTTGAGATATTCCCCAAGACAA

GTTCATGGCCCAATCATGACTCGAACAAAGGTGTAACGACAGCATGT

CCTCATGCTGGAGCAAAAAGCTTCTACAAAAATTTAATATGGCTAGTT

AAAAAAGGAAATTCATACCCAAAGCTCAGCAAATCCTACATTAATGAT

AAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTCACCATCCACCTAC

TAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTTT

TGTGGGGACATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAA

TAAGGCCCAAAGTGAGGGATCAAGAAGGGAGAATGAACTATTACTGG

ACACTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAA

TCTAGTGGTACCGAGATATGCATTCGCAATGGAAAGAAATGCTGGAT

CTGGTATTATCATTTCAGATACACCAGTCCACGATTGCAATACAACTT

GTCAGACACCCAAGGGTGCTATAAACACCAGCCTCCCATTTCAGAAC

ATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGCACA

AAATTGAGACTGGCCACAGGATTGAGGAATGTCCCGTCCATTCAATC

TAGAGGCCTATTTGGGGCCATTGCCGGTTTCATTGAAGGGGGGTGG

ACAGGGATGGTAGATGGATGGTACGGTTATCACCATCAAAATGAGCA

GGGGTCAGGATATGCAGCCGACCTGAAGAGCACACAGAATGCCATT

GACGAGATTACTAACAAAGTAAACTCTGTTATTGAAAAAATGAATACA

CAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAAT

AGAGAATTTAAATAAAAAAGTTGATGATGGTTTCCTGGACATTTGGAC

TTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGA

CTACCACGATTCAAAGGTGAAGAACTTATATGAAAAGGTAAGAAGCC

AGTTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTT

ACCACAAATGTGATAACACGTGCATGGAAAGTGTCAAAAATGGGACT

TATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAA

ATAGATGGGGTAAAGCTGGAATCAACAAGGATTTACCAGATTTTGGC

GATCTATTCAACCGTCGCCAGTTCATTGGTACTGGTAGTCTCCCTGG

GGGCAATCAGTTTCTGGATGTGCTCTAATGGGTCTCTACAGTGTAGA

ATATGTATTTAAGGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAA

GCAGGAGATGTTGAAGAAAACCCCGGGCCTAGCAAAAGCAGGGGAT

AATTCTATTAACCATGAAGACTATCATTGCTTTGAGCTACATTCTATGT

CTGGTTTTCGCTCAAAAACTTCCTGGAAATGACAATAGCACTGCAAC

GCTGTGCCTTGGGCACCATGCAGTACCAAACGGAACGATAGTGAAAA

CAATCACGAATGACCAAATTGAAGTTACTAATGCTACTGAGCTGGTTC

AGAATTCCTCAATAGGTGAAATATGCGACAGTCCTCATCAGATCCTTG

ATGGAGAAAACTGCACACTAATAGATGCTCTATTGGGAGACCCTCAG

TGTGATGGCTTTCAAAATAAGAAATGGGACCTTTTTGTTGAACGAAGC

AAAGCCTACAGCAACTGTTACCCTTATGATGTGCCGGATTATGCCTC

CCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG

AAAGCTTCAATTGGGCTGGAGTCACTCAAAACGGAACAAGTTCTGCT

TGCATAAGGGGATCTAATAGTAGTTTCTTTAGTAGATTAAATTGGTTG

ACCCACTTAAACTTCAAGTACCCAGCATTGAACGTGACTATGCCAAAC

AATGAACAATTTGACAAATTGTACATTTGGGGGGTTCACCACCCGGG

TACGGACAAGGACCAAATCTTCCTGTATGCTCAATCATCAGGAAGAA

TCACAGTATCTACGAAAAGAAGCCAACAAGCTGTAATGCGGAATATC

GGATCTAGACCCAGAATAAGGAATATCCCTAGCAGAATAAGCATCTA

TTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAG

GGAATCTAATTGCTCCTAGGGGTTACTTCAAAATACGAAGTGGGAAA

AGCTCAATAATGAGATCAGATGCACCCATTGGCAAATGCAAGTCTGA

ATGCATCACTCCAAATGGAAGCATTCCCAATGACAAACCATTCCAAAA

TGTAAACAGGATCACATACGGGGCCTGTCCCAGATATGTTAAGCAAA

GCACTCTGAAATTGGCAACAGGAATGCGAAATGTACCAGAGAAACAA

ACTAGAGGCATATTTGGCGCAATAGCGGGTTTCATAGAAAATGGTTG

GGAGGGAATGGTGGATGGTTGGTACGGTTTCAGGCATCAAAATTCTG

AGGGAAGAGGACAAGCAGCAGATCTCAAAAGCACTCAAGCAGCAAT

CGATCAAATCAATGGGAAGCTGAATCGATTGATCGGGAAAACCAACG

AGAAATTCCATCAGATTGAGAAAGAATTCTCAGAAGTAGAAGGGAGA

ATTCAGGACCTTGAGAAATATGTTGAGGACACAAAAATAGATCTCTGG

TCATACAACGCAGAGCTTCTTGTTGCCCTGGAAAACCAACATACAATT

GATCTAACTGACTCAGAAATGAACAAACTGTTTGAAAAAACAAAGAAG

CAACTGAGGGAAAATGCTGAGGATATGGGCAATGGTTGTTTCAAAAT

ATACCACAAATGTGACAATGCCTGCATAGGATCAATCAGAAATGGAA

CTTATGACCACGATGTATACAGGGATGAAGCATTAAACAACCGGTTC

CAGATCAAGGGAGTTGAGCTGAAGTCAGGGTACAAAGATTGGATCCT

ATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTG

GGGTTCATCATGTGGGCCTGCCAAAAGGGCAACATTAGGTGCAACAT

TTGCATTTGAGTGCATTAATTAAAAACACCCTTGTTTCTACTGGAAGC

GGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGA

AAACCCCGGGCCTATTTTCTAATATCCACAAAATGAAGGCAATAATTG

TACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGG

ATAACATCGTCAAACTCACCACATGTCGTCAAAACTGCTACTCAAGG

GGAGGTCAACGTGACCGGTGTAATACCACTGACAACAACACCCACCA

AATCTCATTTTGCAAATCTCAAAGGAACAGAAACCAGGGGGAAACTAT

GCCCAAAATGCCTCAACTGCACAGATCTGGATGTAGCCTTGGGCAGA

CCAAAATGCACAGGGAAAATACCCTCTGCAAGGGTTTCAATACTCCA

TGAAGTCAGACCTGTTACATCTGGGTGCTTTCCTATAATGCACGATAG

AACAAAAATTAGACAGCTGCCTAACCTTCTCCGAGGATACGAACATG

TCAGGTTATCAACTCACAACGTTATCAATGCAAAAGATGCACCAGGAA

GACCCTACGAAATTGGAACCTCAGGGTCTTGCCCTAACATTACCAAT

GGAAACGGATTCTTCGCAACAATGGCTTGGGCCGTCCCAAAAAACAA

AACAGCAACAAATCCATTAACAATAGAAGTACCATACATTTGTACAGA

AGGAGAAGACCAAATTACCGTTTGGGGGTTCCACTCTGACAACGAGA

CCCAAATGGCAAAGCTCTATGGGGACTCAAAGCCCCAGAAGTTCACC

TCATCTGCCAACGGAGTGACCACACATTACGTTTCACAGATTGGTGG

CTTCCCAAATCAAACAGAAGACGGAGGACTACCACAAAGTGGCAGAA

TTGTTGTTGATTACATGGTGCAGAAATCTGGAAAAACAGGAACAATTA

CCTATCAAAGAGGTATTTTATTGCCTCAAAAAGTGTGGTGCGCAAGT

GGCAGGAGCAAGGTAATAAAAGGATCCTTGCCCTTAATTGGAGAAGC

AGATTGCCTCCATGAAAAATACGGTGGATTAAACAAAAGCAAGCCTTA

CTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG

TGAAAACACCCTTGAAGCTGGCCAATGGAACCAAATATAGACCCCCT

GCAAAACTATTAAAGGAAAGAGGTTTCTTCGGAGCCATTGCTGGTTT

CTTAGAGGGAGGATGGGAAGGAATGATTGCAGGTTGGCACGGATAC

ACATCCCATGGGGCACATGGAGTAGCGGTGGCAGCTGACCTTAAGA

GCACTCAAGAGGCCATAAAGAAGATAACAAAAAATCTCAACTCTTTGA

GTGAGCTGGAAGTAAAGAATCTTCAAAGACTAAGCGGTGCCATGGAT

GAACTCCACAACGAAATACTAGAACTAGATGAGAAAGTGGATGATCT

CAGAGCTGACACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT

CCAATGAAGGAATAATAAACAGTGAAGATGAACATCTCTTGGCGCTT

GAAAGAAAGCTGAAGAAAATGCTGGGCCCCTCTGCTGTAGAGATAG

GGAATGGATGCTTTGAAACCAAACACAAGTGCAACCAGACCTGCCTC

GACAGAATAGCTGCTGGTACCTTTGATGCAGGAGAATTTTCTCTCCC

CACCTTTGATTCACTGAATATTACTGCTGCATCTTTAAATGACGACGG

ATTGGACAATCATACTATACTGCTTTACTACTCAACTGCTGCCTCCAG

TTTGGCTGTAACACTGATGATAGCTATCTTTGTTGTTTATATGGTCTC

CAGAGACAATGTTTCTTGCTCCATTTGTCTATAAGGAAAGTTAAGCCC

TGTATTTTCCTTTATTGTAGTGCTTGTTTGCTTGTTGTCATTACAAAGA

AACGTTATTGAAAAATGGAAGCGGAGCCACGAACTTCTCTCTGTTAAA

GCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTTTCGGAGCTATT

GCTGGTTTCTTGGAAGGAGGATGGGAAGGAATGATTGCAGGTTGGC

ACGGATACACATCTCATGGAGCACATGGAGTAGCAGTGGCAGCAGA

CCTTAAGAGTACCCATGATGA

Pan-CoV-Flu
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCG
413

Vaccine #25
TGAACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAG

CoV-Wuhan
CTTCACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGC

Spike(HexaPro)-
GTGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGT

Influenza (HA)
GACCTGGTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAG

AGGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCG

CCAGCACCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCAC

CACCCTGGACAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCC

ACCAACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCC

CTTCCTGGGCGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGA

GCGAGTTCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTA

CGTGAGCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAAC

TTCAAGAACCTGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTT

CAAGATCTACAGCAAGCACACCCCCATCAACCTGGTGAGGGACCTG

CCCCAGGGCTTCAGCGCCCTGGAGCCCCTGGTGGACCTGCCCATCG

GCATCAACATCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAG

CTACCTGACCCCCGGCGACAGCAGCAGCGGCTGGACCGCCGGCGC

CGCCGCCTACTACGTGGGCTACCTGCAGCCCAGGACCTTCCTGCTG

AAGTACAACGAGAACGGCACCATCACCGACGCCGTGGACTGCGCCC

TGGACCCCCTGAGCGAGACCAAGTGCACCCTGAAGAGCTTCACCGT

GGAGAAGGGCATCTACCAGACCAGCAACTTCAGGGTGCAGCCCACC

GAGAGCATCGTGAGGTTCCCCAACATCACCAACCTGTGCCCCTTCG

GCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGTACGCCTGGAA

CAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTAC

AACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTGAGCCCCA

CCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTC

GTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCAGACC

GGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCACCG

GCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGGG

CGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAG

CACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTG

CAGAGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCT

ACAGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCAC

CGTGTGCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGC

GTGAACTTCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCG

AGAGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACAT

CGCCGACACCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGAT

CCTGGACATCACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACC

CCCGGCACCAACACCAGCAACCAGGTGGCCGTGCTGTACCAGGACG

TGAACTGCACCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGAC

CCCCACCTGGAGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACC

AGGGCCGGCTGCCTGATCGGCGCCGAGCACGTGAACAACAGCTAC

GAGTGCGACATCCCCATCGGCGCCGGCATCTGCGCCAGCTACCAGA

CCCAGACCAACAGCCCCGGCAGCGCCAGCAGCGTGGCCAGCCAGA

GCATCATCGCCTACACCATGAGCCTGGGCGCCGAGAACAGCGTGGC

CTACAGCAACAACAGCATCGCCATCCCCACCAACTTCACCATCAGCG

TGACCACCGAGATCCTGCCCGTGAGCATGACCAAGACCAGCGTGGA

CTGCACCATGTACATCTGCGGCGACAGCACCGAGTGCAGCAACCTG

CTGCTGCAGTACGGCAGCTTCTGCACCCAGCTGAACAGGGCCCTGA

CCGGCATCGCCGTGGAGCAGGACAAGAACACCCAGGAGGTGTTCGC

CCAGGTGAAGCAGATCTACAAGACCCCCCCCATCAAGGACTTCGGC

GGCTTCAACTTCAGCCAGATCCTGCCCGACCCCAGCAAGCCCAGCA

AGAGGAGCCCCATCGAGGACCTGCTGTTCAACAAGGTGACCCTGGC

CGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCTGGGCGACATC

GCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACGGCCTGACCG

TGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAGTACACCAG

CGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTTCGGCGC

CGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATGGCCTACAGG

TTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACCAGA

AGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGGA

CAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGT

GGTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTG

AGCAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGA

GCAGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGA

TCACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCT

GATCAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCAC

CAAGATGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTC

TGCGGCAAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCC

ACGGCGTGGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAA

GAACTTCACCACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCAC

TTCCCCAGGGAGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCG

TGACCCAGAGGAACTTCTACGAGCCCCAGATCATCACCACCGACAAC

ACCTTCGTGAGCGGCAACTGCGACGTGGTGATCGGCATCGTGAACA

ACACCGTGTACGACCCCCTGCAGCCCGAGCTGGACAGCTTCAAGGA

GGAGCTGGACAAGTACTTCAAGAACCACACCAGCCCCGACGTGGAC

CTGGGCGACATCAGCGGCATCAACGCCAGCGTGGTGAACATCCAGA

AGGAGATCGACAGGCTGAACGAGGTGGCCAAGAACCTGAACGAGAG

CCTGATCGACCTGCAGGAGCTGGGCAAGTACGAGCAGTACATCAAG

TGGCCCTGGTACATCTGGCTGGGCTTCATCGCCGGCCTGATCGCCA

TCGTGATGGTGACCATCATGCTGTGCTGCATGACCAGCTGCTGCAGC

TGCCTGAAGGGCTGCTGCAGCTGCGGCAGCTGCTGCAAGTTCGACG

AGGACGACAGCGAGCCCGTGCTGAAGGGCGTGAAGCTGCACTACAC

CGGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGAT

GTTGAAGAAAACCCCGGGCCTATGAAGGCAATACTAGTAGTTCTGCT

ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCA

TGCGAACAACTCAACAGACACTGTAGACACAGTACTAGAAAAGAATG

TAACAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGA

AACTATGCAAACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGT

AACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCTC

CACAGCAAGCTCATGGTCCTACATTGTGGAAACATCTAGTTCAGACA

ATGGAACGTGTTACCCAGGAGATTTCATCGATTATGAGGAGCTAAGA

GAGCAATTGAGCTCAGTGTCATCATTTGAAAGGTTTGAGATATTCCCC

AAGACAAGTTCATGGCCCAATCATGACTCGAACAAAGGTGTAACGAC

AGCATGTCCTCATGCTGGAGCAAAAAGCTTCTACAAAAATTTAATATG

GCTAGTTAAAAAAGGAAATTCATACCCAAAGCTCAGCAAATCCTACAT

TAATGATAAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTCACCATC

CACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCA

TATGTTTTTGTGGGGACATCAAGATACAGCAAGAAGTTCAAGCCGGA

AATAGCAATAAGGCCCAAAGTGAGGGATCAAGAAGGGAGAATGAACT

ATTACTGGACACTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCA

ACTGGAAATCTAGTGGTACCGAGATATGCATTCGCAATGGAAAGAAA

TGCTGGATCTGGTATTATCATTTCAGATACACCAGTCCACGATTGCAA

TACAACTTGTCAGACACCCAAGGGTGCTATAAACACCAGCCTCCCAT

TTCAGAACATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAA

AAAGCACAAAATTGAGACTGGCCACAGGATTGAGGAATGTCCCGTCC

ATTCAATCTAGAGGCCTATTTGGGGCCATTGCCGGTTTCATTGAAGG

GGGGTGGACAGGGATGGTAGATGGATGGTACGGTTATCACCATCAA

AATGAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACACAGA

ATGCCATTGACGAGATTACTAACAAAGTAAACTCTGTTATTGAAAAAA

TGAATACACAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAA

AAAAGAATAGAGAATTTAAATAAAAAAGTTGATGATGGTTTCCTGGAC

ATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGA

ACTTTGGACTACCACGATTCAAAGGTGAAGAACTTATATGAAAAGGTA

AGAAGCCAGTTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTT

TGAATTTTACCACAAATGTGATAACACGTGCATGGAAAGTGTCAAAAA

TGGGACTTATGACTACCGAAAATACTCAGAGGAAGCAAAATTAAACA

GAGAAGAAATAGATGGGGTAAAGCTGGAATCAACAAGGATTTACCAG

ATTTTGGCGATCTATTCAACCGTCGCCAGTTCATTGGTACTGGTAGTC

TCCCTGGGGGCAATCAGTTTCTGGATGTGCTCTAATGGGTCTCTACA

GTGTAGAATATGTATTTAAGGAAGCGGAGCCACGAACTTCTCTCTGTT

AAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTAGCAAAAGC

AGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTTGAGCTACA

TTCTATGTCTGGTTTTCGCTCAAAAACTTCCTGGAAATGACAATAGCA

CTGCAACGCTGTGCCTTGGGCACCATGCAGTACCAAACGGAACGAT

AGTGAAAACAATCACGAATGACCAAATTGAAGTTACTAATGCTACTGA

GCTGGTTCAGAATTCCTCAATAGGTGAAATATGCGACAGTCCTCATC

AGATCCTTGATGGAGAAAACTGCACACTAATAGATGCTCTATTGGGA

GACCCTCAGTGTGATGGCTTTCAAAATAAGAAATGGGACCTTTTTGTT

GAACGAAGCAAAGCCTACAGCAACTGTTACCCTTATGATGTGCCGGA

TTATGCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGT

TTAACAATGAAAGCTTCAATTGGGCTGGAGTCACTCAAAACGGAACA

AGTTCTGCTTGCATAAGGGGATCTAATAGTAGTTTCTTTAGTAGATTA

AATTGGTTGACCCACTTAAACTTCAAGTACCCAGCATTGAACGTGACT

ATGCCAAACAATGAACAATTTGACAAATTGTACATTTGGGGGGTTCAC

CACCCGGGTACGGACAAGGACCAAATCTTCCTGTATGCTCAATCATC

AGGAAGAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCC

CGAATATGGGATCTAGACCCAGAATAAGGAATATCCCTAGCAGAATA

AGCATCTATTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAAC

AGCACAGGGAATCTAATTGCTCCTAGGGGTTACTTCAAAATACGAAG

TGGGAAAAGCTCAATAATGAGATCAGATGCACCCATTGGCAAATGCA

AGTCTGAATGCATCACTCCAAATGGAAGCATTCCCAATGACAAACCAT

TCCAAAATGTAAACAGGATCACATACGGGGCCTGTCCCAGATATGTT

AAGCAAAGCACTCTGAAATTGGCAACAGGAATGCGAAATGTACCAGA

GAAACAAACTAGAGGCATATTTGGCGCAATAGCGGGTTTCATAGAAA

ATGGTTGGGAGGGAATGGTGGATGGTTGGTACGGTTTCAGGCATCA

AAATTCTGAGGGAAGAGGACAAGCAGCAGATCTCAAAAGCACTCAAG

CAGCAATCGATCAAATCAATGGGAAGCTGAATCGATTGATCGGGAAA

ACCAACGAGAAATTCCATCAGATTGAGAAAGAATTCTCAGAAGTAGAA

GGGAGAATTCAGGACCTTGAGAAATATGTTGAGGACACAAAAATAGA

TCTCTGGTCATACAACGCAGAGCTTCTTGTTGCCCTGGAAAACCAAC

ATACAATTGATCTAACTGACTCAGAAATGAACAAACTGTTTGAAAAAA

CAAAGAAGCAACTGAGGGAAAATGCTGAGGATATGGGCAATGGTTGT

TTCAAAATATACCACAAATGTGACAATGCCTGCATAGGATCAATCAGA

AATGGAACTTATGACCACGATGTATACAGGGATGAAGCATTAAACAA

CCGGTTCCAGATCAAGGGAGTTGAGCTGAAGTCAGGGTACAAAGATT

GGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGC

TTTGTTGGGGTTCATCATGTGGGCCTGCCAAAAGGGCAACATTAGGT

GCAACATTTGCATTTGAGTGCATTAATTAAAAACACCCTTGTTTCTACT

GGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGT

TGAAGAAAACCCCGGGCCTATTTTCTAATATCCACAAAATGAAGGCAA

TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCA

CTGGGATAACATCGTCAAACTCACCACATGTCGTCAAAACTGCTACT

CAAGGGGAGGTCAACGTGACCGGTGTAATACCACTGACAACAACAC

CCACCAAATCTCATTTTGCAAATCTCAAAGGAACAGAAACCAGGGGG

AAACTATGCCCAAAATGCCTCAACTGCACAGATCTGGATGTAGCCTT

GGGCAGACCAAAATGCACAGGGAAAATACCCTCTGCAAGGGTTTCAA

TACTCCATGAAGTCAGACCTGTTACATCTGGGTGCTTTCCTATAATGC

ACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTCCGAGGATAC

GAACATGTCAGGTTATCAACTCACAACGTTATCAATGCAAAAGATGCA

CCAGGAAGACCCTACGAAATTGGAACCTCAGGGTCTTGCCCTAACAT

TACCAATGGAAACGGATTCTTCGCAACAATGGCTTGGGCCGTCCCAA

AAAACAAAACAGCAACAAATCCATTAACAATAGAAGTACCATACATTT

GTACAGAAGGAGAAGACCAAATTACCGTTTGGGGGTTCCACTCTGAC

AACGAGACCCAAATGGCAAAGCTCTATGGGGACTCAAAGCCCCAGA

AGTTCACCTCATCTGCCAACGGAGTGACCACACATTACGTTTCACAG

ATTGGTGGCTTCCCAAATCAAACAGAAGACGGAGGACTACCACAAAG

TGGCAGAATTGTTGTTGATTACATGGTGCAGAAATCTGGAAAAACAG

GAACAATTACCTATCAAAGAGGTATTTTATTGCCTCAAAAAGTGTGGT

GCGCAAGTGGCAGGAGCAAGGTAATAAAAGGATCCTTGCCCTTAATT

GGAGAAGCAGATTGCCTCCATGAAAAATACGGTGGATTAAACAAAAG

CAAGCCTTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCC

CAATATGGGTGAAAACACCCTTGAAGCTGGCCAATGGAACCAAATAT

AGACCCCCTGCAAAACTATTAAAGGAAAGAGGTTTCTTCGGAGCCAT

TGCTGGTTTCTTAGAGGGAGGATGGGAAGGAATGATTGCAGGTTGG

CACGGATACACATCCCATGGGGCACATGGAGTAGCGGTGGCAGCTG

ACCTTAAGAGCACTCAAGAGGCCATAAACAAGATAACAAAAAATCTCA

ACTCTTTGAGTGAGCTGGAAGTAAAGAATCTTCAAAGACTAAGCGGT

GCCATGGATGAACTCCACAACGAAATACTAGAACTAGATGAGAAAGT

GGATGATCTCAGAGCTGACACAATAAGCTCACAAATAGAACTCGCAG

TCCTGCTTTCCAATGAAGGAATAATAAACAGTGAAGATGAACATCTCT

TGGCGCTTGAAAGAAAGCTGAAGAAAATGCTGGGCCCCTCTGCTGTA

GAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCAGAC

CTGCCTCGACAGAATAGCTGCTGGTACCTTTGATGCAGGAGAATTTT

CTCTCCCCACCTTTGATTCACTGAATATTACTGCTGCATCTTTAAATG

ACGACGGATTGGACAATCATACTATACTGCTTTACTACTCAACTGCTG

CCTCCAGTTTGGCTGTAACACTGATGATAGCTATCTTTGTTGTTTATA

TGGTCTCCAGAGACAATGTTTCTTGCTCCATTTGTCTATAAGGAAAGT

TAAGCCCTGTATTTTCCTTTATTGTAGTGCTTGTTTGCTTGTTGTCATT

ACAAAGAAACGTTATTGAAAAATGGAAGCGGAGCCACGAACTTCTCT

CTGTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTTTCGG

AGCTATTGCTGGTTTCTTGGAAGGAGGATGGGAAGGAATGATTGCAG

GTTGGCACGGATACACATCTCATGGAGCACATGGAGTAGCAGTGGC

AGCAGACCTTAAGAGTACCCATGATGA

Pan-CoV-Flu
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCG
414

Vaccine #26
TGAACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAG

CoV-Wuhan
CTTCACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGC

Spike(2 proline
GTGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGT

substitution)-
GACCTGGTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAG

Influenza (HA)
AGGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCG

CCAGCACCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCAC

CACCCTGGACAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCC

ACCAACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCC

CTTCCTGGGCGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGA

GCGAGTTCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTA

CGTGAGCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAAC

TTCAAGAACCTGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTT

CAAGATCTACAGCAAGCACACCCCCATCAACCTGGTGAGGGACCTG

CCCCAGGGCTTCAGCGCCCTGGAGCCCCTGGTGGACCTGCCCATCG

GCATCAACATCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAG

CTACCTGACCCCCGGCGACAGCAGCAGCGGCTGGACCGCCGGCGC

CGCCGCCTACTACGTGGGCTACCTGCAGCCCAGGACCTTCCTGCTG

AAGTACAACGAGAACGGCACCATCACCGACGCCGTGGACTGCGCCC

TGGACCCCCTGAGCGAGACCAAGTGCACCCTGAAGAGCTTCACCGT

GGAGAAGGGCATCTACCAGACCAGCAACTTCAGGGTGCAGCCCACC

GAGAGCATCGTGAGGTTCCCCAACATCACCAACCTGTGCCCCTTCG

GCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGTACGCCTGGAA

CAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTAC

AACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTGAGCCCCA

CCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTC

GTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCAGACC

GGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCACCG

GCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGGG

CGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAG

CACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTG

CAGAGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCT

ACAGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCAC

CGTGTGCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGC

GTGAACTTCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCG

AGAGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACAT

CGCCGACACCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGAT

CCTGGACATCACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACC

CCCGGCACCAACACCAGCAACCAGGTGGCCGTGCTGTACCAGGACG

TGAACTGCACCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGAC

CCCCACCTGGAGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACC

AGGGCCGGCTGCCTGATCGGCGCCGAGCACGTGAACAACAGCTAC

GAGTGCGACATCCCCATCGGCGCCGGCATCTGCGCCAGCTACCAGA

CCCAGACCAACAGCCCCAGGAGGGCCAGGAGCGTGGCCAGCCAGA

GCATCATCGCCTACACCATGAGCCTGGGCGCCGAGAACAGCGTGGC

CTACAGCAACAACAGCATCGCCATCCCCACCAACTTCACCATCAGCG

TGACCACCGAGATCCTGCCCGTGAGCATGACCAAGACCAGCGTGGA

CTGCACCATGTACATCTGCGGCGACAGCACCGAGTGCAGCAACCTG

CTGCTGCAGTACGGCAGCTTCTGCACCCAGCTGAACAGGGCCCTGA

CCGGCATCGCCGTGGAGCAGGACAAGAACACCCAGGAGGTGTTCGC

CCAGGTGAAGCAGATCTACAAGACCCCCCCCATCAAGGACTTCGGC

GGCTTCAACTTCAGCCAGATCCTGCCCGACCCCAGCAAGCCCAGCA

AGAGGAGCTTCATCGAGGACCTGCTGTTCAACAAGGTGACCCTGGC

CGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCTGGGCGACATC

GCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACGGCCTGACCG

TGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAGTACACCAG

CGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTTCGGCGC

CGGCGCCGCCCTGCAGATCCCCTTCGCCATGCAGATGGCCTACAGG

TTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACCAGA

AGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGGA

CAGCCTGAGCAGCACCGCCAGCGCCCTGGGCAAGCTGCAGGACGT

GGTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTG

AGCAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGA

GCAGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGA

TCACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCT

GATCAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCAC

CAAGATGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTC

TGCGGCAAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCC

ACGGCGTGGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAA

GAACTTCACCACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCAC

TTCCCCAGGGAGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCG

TGACCCAGAGGAACTTCTACGAGCCCCAGATCATCACCACCGACAAC

ACCTTCGTGAGCGGCAACTGCGACGTGGTGATCGGCATCGTGAACA

ACACCGTGTACGACCCCCTGCAGCCCGAGCTGGACAGCTTCAAGGA

GGAGCTGGACAAGTACTTCAAGAACCACACCAGCCCCGACGTGGAC

CTGGGCGACATCAGCGGCATCAACGCCAGCGTGGTGAACATCCAGA

AGGAGATCGACAGGCTGAACGAGGTGGCCAAGAACCTGAACGAGAG

CCTGATCGACCTGCAGGAGCTGGGCAAGTACGAGCAGTACATCAAG

TGGCCCTGGTACATCTGGCTGGGCTTCATCGCCGGCCTGATCGCCA

TCGTGATGGTGACCATCATGCTGTGCTGCATGACCAGCTGCTGCAGC

TGCCTGAAGGGCTGCTGCAGCTGCGGCAGCTGCTGCAAGTTCGACG

AGGACGACAGCGAGCCCGTGCTGAAGGGCGTGAAGCTGCACTACAC

CGGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGAT

GTTGAAGAAAACCCCGGGCCTATGAAGGCAATACTAGTAGTTCTGCT

ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCA

TGCGAACAACTCAACAGACACTGTAGACACAGTACTAGAAAAGAATG

TAACAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGA

AACTATGCAAACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGT

AACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCTC

CACAGCAAGCTCATGGTCCTACATTGTGGAAACATCTAGTTCAGACA

ATGGAACGTGTTACCCAGGAGATTTCATCGATTATGAGGAGCTAAGA

GAGCAATTGAGCTCAGTGTCATCATTTGAAAGGTTTGAGATATTCCCC

AAGACAAGTTCATGGCCCAATCATGACTCGAACAAAGGTGTAACGAC

AGCATGTCCTCATGCTGGAGCAAAAAGCTTCTACAAAAATTTAATATG

GCTAGTTAAAAAAGGAAATTCATACCCAAAGCTCAGCAAATCCTACAT

TAATGATAAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTCACCATC

CACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCA

TATGTTTTTGTGGGGACATCAAGATACAGCAAGAAGTTCAAGCCGGA

AATAGCAATAAGGCCCAAAGTGAGGGATCAAGAAGGGAGAATGAACT

ATTACTGGACACTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCA

ACTGGAAATCTAGTGGTACCGAGATATGCATTCGCAATGGAAAGAAA

TGCTGGATCTGGTATTATCATTTCAGATACACCAGTCCACGATTGCAA

TACAACTTGTCAGACACCCAAGGGTGCTATAAACACCAGCCTCCCAT

TTCAGAACATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAA

AAAGCACAAAATTGAGACTGGCCACAGGATTGAGGAATGTCCCGTCC

ATTCAATCTAGAGGCCTATTTGGGGCCATTGCCGGTTTCATTGAAGG

GGGGTGGACAGGGATGGTAGATGGATGGTACGGTTATCACCATCAA

AATGAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACACAGA

ATGCCATTGACGAGATTACTAACAAAGTAAACTCTGTTATTGAAAAAA

TGAATACACAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAA

AAAAGAATAGAGAATTTAAATAAAAAAGTTGATGATGGTTTCCTGGAC

ATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGA

ACTTTGGACTACCACGATTCAAAGGTGAAGAACTTATATGAAAAGGTA

AGAAGCCAGTTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTT

TGAATTTTACCACAAATGTGATAACACGTGCATGGAAAGTGTCAAAAA

TGGGACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACA

GAGAAGAAATAGATGGGGTAAAGCTGGAATCAACAAGGATTTACCAG

ATTTTGGCGATCTATTCAACCGTCGCCAGTTCATTGGTACTGGTAGTC

TCCCTGGGGGCAATCAGTTTCTGGATGTGCTCTAATGGGTCTCTACA

GTGTAGAATATGTATTTAAGGAAGCGGAGCCACGAACTTCTCTCTGTT

AAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTAGCAAAAGC

AGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTTGAGCTACA

TTCTATGTCTGGTTTTCGCTCAAAAACTTCCTGGAAATGACAATAGCA

CTGCAACGCTGTGCCTTGGGCACCATGCAGTACCAAACGGAACGAT

AGTGAAAACAATCACGAATGACCAAATTGAAGTTACTAATGCTACTGA

GCTGGTTCAGAATTCCTCAATAGGTGAAATATGCGACAGTCCTCATC

AGATCCTTGATGGAGAAAACTGCACACTAATAGATGCTCTATTGGGA

GACCCTCAGTGTGATGGCTTTCAAAATAAGAAATGGGACCTTTTTGTT

GAACGAAGCAAAGCCTACAGCAACTGTTACCCTTATGATGTGCCGGA

TTATGCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGT

TTAACAATGAAAGCTTCAATTGGGCTGGAGTCACTCAAAACGGAACA

AGTTCTGCTTGCATAAGGGGATCTAATAGTAGTTTCTTTAGTAGATTA

AATTGGTTGACCCACTTAAACTTCAAGTACCCAGCATTGAACGTGACT

ATGCCAAACAATGAACAATTTGACAAATTGTACATTTGGGGGGTTCAC

CACCCGGGTACGGACAAGGACCAAATCTTCCTGTATGCTCAATCATC

AGGAAGAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCC

CGAATATCGGATCTAGACCCAGAATAAGGAATATCCCTAGCAGAATA

AGCATCTATTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAAC

AGCACAGGGAATCTAATTGCTCCTAGGGGTTACTTCAAAATACGAAG

TGGGAAAAGCTCAATAATGAGATCAGATGCACCCATTGGCAAATGCA

AGTCTGAATGCATCACTCCAAATGGAAGCATTCCCAATGACAAACCAT

TCCAAAATGTAAACAGGATCACATACGGGGCCTGTCCCAGATATGTT

AAGCAAAGCACTCTGAAATTGGCAACAGGAATGCGAAATGTACCAGA

GAAACAAACTAGAGGCATATTTGGCGCAATAGCGGGTTTCATAGAAA

ATGGTTGGGAGGGAATGGTGGATGGTTGGTACGGTTTCAGGCATCA

AAATTCTGAGGGAAGAGGACAAGCAGCAGATCTCAAAAGCACTCAAG

CAGCAATCGATCAAATCAATGGGAAGCTGAATCGATTGATCGGGAAA

ACCAACGAGAAATTCCATCAGATTGAGAAAGAATTCTCAGAAGTAGAA

GGGAGAATTCAGGACCTTGAGAAATATGTTGAGGACACAAAAATAGA

TCTCTGGTCATACAACGCAGAGCTTCTTGTTGCCCTGGAAAACCAAC

ATACAATTGATCTAACTGACTCAGAAATGAACAAACTGTTTGAAAAAA

CAAAGAAGCAACTGAGGGAAAATGCTGAGGATATGGGCAATGGTTGT

TTCAAAATATACCACAAATGTGACAATGCCTGCATAGGATCAATCAGA

AATGGAACTTATGACCACGATGTATACAGGGATGAAGCATTAAACAA

CCGGTTCCAGATCAAGGGAGTTGAGCTGAAGTCAGGGTACAAAGATT

GGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGC

TTTGTTGGGGTTCATCATGTGGGCCTGCCAAAAGGGCAACATTAGGT

GCAACATTTGCATTTGAGTGCATTAATTAAAAACACCCTTGTTTCTACT

GGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGT

TGAAGAAAACCCCGGGCCTATTTTCTAATATCCACAAAATGAAGGCAA

TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCA

CTGGGATAACATCGTCAAACTCACCACATGTCGTCAAAACTGCTACT

CAAGGGGAGGTCAACGTGACCGGTGTAATACCACTGACAACAACAC

CCACCAAATCTCATTTTGCAAATCTCAAAGGAACAGAAACCAGGGGG

AAACTATGCCCAAAATGCCTCAACTGCACAGATCTGGATGTAGCCTT

GGGCAGACCAAAATGCACAGGGAAAATACCCTCTGCAAGGGTTTCAA

TACTCCATGAAGTCAGACCTGTTACATCTGGGTGCTTTCCTATAATGC

ACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTCCGAGGATAC

GAACATGTCAGGTTATCAACTCACAACGTTATCAATGCAAAAGATGCA

CCAGGAAGACCCTACGAAATTGGAACCTCAGGGTCTTGCCCTAACAT

TACCAATGGAAACGGATTCTTCGCAACAATGGCTTGGGCCGTCCCAA

AAAACAAAACAGCAACAAATCCATTAACAATAGAAGTACCATACATTT

GTACAGAAGGAGAAGACCAAATTACCGTTTGGGGGTTCCACTCTGAC

AACGAGACCCAAATGGCAAAGCTCTATGGGGACTCAAAGCCCCAGA

AGTTCACCTCATCTGCCAACGGAGTGACCACACATTACGTTTCACAG

ATTGGTGGCTTCCCAAATCAAACAGAAGACGGAGGACTACCACAAAG

TGGCAGAATTGTTGTTGATTACATGGTGCAGAAATCTGGAAAAACAG

GAACAATTACCTATCAAAGAGGTATTTTATTGCCTCAAAAAGTGTGGT

GCGCAAGTGGCAGGAGCAAGGTAATAAAAGGATCCTTGCCCTTAATT

GGAGAAGCAGATTGCCTCCATGAAAAATACGGTGGATTAAACAAAAG

CAAGCCTTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCC

CAATATGGGTGAAAACACCCTTGAAGCTGGCCAATGGAACCAAATAT

AGACCCCCTGCAAAACTATTAAAGGAAAGAGGTTTCTTCGGAGCCAT

TGCTGGTTTCTTAGAGGGAGGATGGGAAGGAATGATTGCAGGTTGG

CACGGATACACATCCCATGGGGCACATGGAGTAGCGGTGGCAGCTG

ACCTTAAGAGCACTCAAGAGGCCATAAACAAGATAACAAAAAATCTCA

ACTCTTTGAGTGAGCTGGAAGTAAAGAATCTTCAAAGACTAAGCGGT

GCCATGGATGAACTCCACAACGAAATACTAGAACTAGATGAGAAAGT

GGATGATCTCAGAGCTGACACAATAAGCTCACAAATAGAACTCGCAG

TCCTGCTTTCCAATGAAGGAATAATAAACAGTGAAGATGAACATCTCT

TGGCGCTTGAAAGAAAGCTGAAGAAAATGCTGGGCCCCTCTGCTGTA

GAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCAGAC

CTGCCTCGACAGAATAGCTGCTGGTACCTTTGATGCAGGAGAATTTT

CTCTCCCCACCTTTGATTCACTGAATATTACTGCTGCATCTTTAAATG

ACGACGGATTGGACAATCATACTATACTGCTTTACTACTCAACTGCTG

CCTCCAGTTTGGCTGTAACACTGATGATAGCTATCTTTGTTGTTTATA

TGGTCTCCAGAGACAATGTTTCTTGCTCCATTTGTCTATAAGGAAAGT

TAAGCCCTGTATTTTCCTTTATTGTAGTGCTTGTTTGCTTGTTGTCATT

ACAAAGAAACGTTATTGAAAAATGGAAGCGGAGCCACGAACTTCTCT

CTGTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTTTCGG

AGCTATTGCTGGTTTCTTGGAAGGAGGATGGGAAGGAATGATTGCAG

GTTGGCACGGATACACATCTCATGGAGCACATGGAGTAGCAGTGGC

AGCAGACCTTAAGAGTACCCATGATGA

Pan-CoV-Flu
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCG
415

Vaccine #27
TGAACCTGATCACCAGGACCCAGAGCTACACCAACAGCTTCACCAGG

CoV- Omicron
GGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCTGCACA

BA.2
GCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTGGTTC

Spike(HexaPra)-
CACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGACA

Influenza (HA)
ACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGAC

AGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGG

TGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGAC

GTGTACTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCA

GGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCA

GCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAAC

CTGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTA

CAGCAAGCACACCCCCATCAACCTGGGCAGGGACCTGCCCCAGGGC

TTCAGCGCCCTGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACA

TCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGAC

CCCCGGCGACAGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTA

CTACGTGGGCTACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAAC

GAGAACGGCACCATCACCGACGCCGTGGACTGCGCCCTGGACCCC

CTGAGCGAGACCAAGTGCACCCTGAAGAGCTTCACCGTGGAGAAGG

GCATCTACCAGACCAGCAACTTCAGGGTGCAGCCCACCGAGAGCAT

CGTGAGGTTCCCCAACATCACCAACCTGTGCCCCTTCGACGAGGTGT

TCAACGCCACCAGGTTCGCCAGCGTGTACGCCTGGAACAGGAAGAG

GATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTACAACTTCGCC

CCCTTCTTCGCCTTCAAGTGCTACGGCGTGAGCCCCACCAAGCTGAA

CGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTCGTGATCAGG

GGCAACGAGGTGAGCCAGATCGCCCCCGGCCAGACCGGCAACATC

GCCGACTACAACTACAAGCTGCCCGACGACTTCACCGGCTGCGTGA

TCGCCTGGAACAGCAACAAGCTGGACAGCAAGGTGGGCGGCAACTA

CAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGAAGCCCTTCG

AGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAACAAGCCCTG

CAACGGCGTGGCCGGCTTCAACTGCTACTTCCCCCTGAGGAGCTAC

GGCTTCAGGCCCACCTACGGCGTGGGCCACCAGCCCTACAGGGTG

GTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGTGCG

GCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACTT

CAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAAC

AAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACA

CCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACAT

CACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACC

AACACCAGCAACCAGGTGGCCGTGCTGTACCAGGGCGTGAACTGCA

CCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTG

GAGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGG

CTGCCTGATCGGCGCCGAGTACGTGAACAACAGCTACGAGTGCGAC

ATCCCCATCGGCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCA

AGAGCCACCAGCAGGCCCAGAGCGTGGCCAGCCAGAGCATCATCG

CCTACACCATGAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAA

CAACAGCATCGCCATCCCCACCAACTTCACCATCAGCGTGACCACCG

AGATCCTGCCCGTGAGCATGACCAAGACCAGCGTGGACTGCACCAT

GTACATCTGCGGCGACAGCACCGAGTGCAGCAACCTGCTGCTGCAG

TACGGCAGCTTCTGCACCCAGCTGAAGAGGGCCCTGACCGGCATCG

CCGTGGAGCAGGACAAGAACACCCAGGAGGTGTTCGCCCAGGTGAA

GCAGATCTACAAGACCCCCCCCATCAAGTACTTCGGCGGCTTCAACT

TCAGCCAGATCCTGCCCGACCCCAGCAAGCCCAGCAAGAGGAGCCC

CATCGAGGACCTGCTGTTCAACAAGGTGACCCTGGCCGACGCCGGC

TTCATCAAGCAGTACGGCGACTGCCTGGGCGACATCGCCGCCAGGG

ACCTGATCTGCGCCCAGAAGTTCAACGGCCTGACCGTGCTGCCCCC

CCTGCTGACCGACGAGATGATCGCCCAGTACACCAGCGCCCTGCTG

GCCGGCACCATCACCAGCGGCTGGACCTTCGGCGCCGGCCCCGCC

CTGCAGATCCCCTTCCCCATGCAGATGGCCTACAGGTTCAACGGCAT

CGGCGTGACCCAGAACGTGCTGTACGAGAACCAGAAGCTGATCGCC

AACCAGTTCAACAGCGCCATCGGCAAGATCCAGGACAGCCTGAGCA

GCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGTGGTGAACCACAA

CGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAGCAGCAAGTTC

GGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAGGCTGGACC

CCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCGGCAGGC

TGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGGGCCGC

CGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAGCGA

GTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGGG

CTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGTG

TTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACCA

CCGCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGGA

GGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAGG

AACTTCTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGAG

CGGCAACTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTAC

GACCCCCTGCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGACA

AGTACTTCAAGAACCACACCAGCCCCGACGTGGACCTGGGCGACAT

CAGCGGCATCAACGCCAGCGTGGTGAACATCCAGAAGGAGATCGAC

AGGCTGAACGAGGTGGCCAAGAACCTGAACGAGAGCCTGATCGACC

TGCAGGAGCTGGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGTA

CATCTGGCTGGGCTTCATCGCCGGCCTGATCGCCATCGTGATGGTG

ACCATCATGCTGTGCTGCATGACCAGCTGCTGCAGCTGCCTGAAGG

GCTGCTGCAGCTGCGGCAGCTGCTGCAAGTTCGACGAGGACGACAG

CGAGCCCGTGCTGAAGGGCGTGAAGCTGCACTACACCGGAAGCGG

AGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAAA

ACCCCGGGCCTATGAAGGCAATACTAGTAGTTCTGCTATATACATTTG

CAACCGCAAATGCAGACACATTATGTATAGGTTATCATGCGAACAACT

CAACAGACACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACA

CACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAA

CTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGCTGG

CTGGATCCTGGGAAATCCAGAGTGTGAATCACTCTCCACAGCAAGCT

CATGGTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTT

ACCCAGGAGATTTCATCGATTATGAGGAGCTAAGAGAGCAATTGAGC

TCAGTGTCATCATTTGAAAGGTTTGAGATATTCCCCAAGACAAGTTCA

TGGCCCAATCATGACTCGAACAAAGGTGTAACGACAGCATGTCCTCA

TGCTGGAGCAAAAAGCTTCTACAAAAATTTAATATGGCTAGTTAAAAA

AGGAAATTCATACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGG

GAAAGAAGTCCTCGTGCTATGGGGCATTCACCATCCACCTACTAGTG

CTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTTTTGTGG

GGACATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGG

CCCAAAGTGAGGGATCAAGAAGGGAGAATGAACTATTACTGGACACT

AGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAG

TGGTACCGAGATATGCATTCGCAATGGAAAGAAATGCTGGATCTGGT

ATTATCATTTCAGATACACCAGTCCACGATTGCAATACAACTTGTCAG

ACACCCAAGGGTGCTATAAACACCAGCCTCCCATTTCAGAACATACA

TCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGCACAAAATT

GAGACTGGCCACAGGATTGAGGAATGTCCCGTCCATTCAATCTAGAG

GCCTATTTGGGGCCATTGCCGGTTTCATTGAAGGGGGGTGGACAGG

GATGGTAGATGGATGGTACGGTTATCACCATCAAAATGAGCAGGGGT

CAGGATATGCAGCCGACCTGAAGAGCACACAGAATGCCATTGACGA

GATTACTAACAAAGTAAACTCTGTTATTGAAAAAATGAATACACAGTTC

ACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGAA

TTTAAATAAAAAAGTTGATGATGGTTTCCTGGACATTTGGACTTACAAT

GCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCA

CGATTCAAAGGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGTTAAA

AAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACCACAA

ATGTGATAACACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACT

ACCCAAAATACTGAGAGGAAGGAAAATTAAACAGAGAAGAAATAGAT

GGGGTAAAGCTGGAATCAACAAGGATTTACCAGATTTTGGCGATCTA

TTCAACCGTCGCCAGTTCATTGGTACTGGTAGTCTCCCTGGGGGCAA

TCAGTTTCTGGATGTGCTCTAATGGGTCTCTACAGTGTAGAATATGTA

TTTAAGGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGA

GATGTTGAAGAAAACCCCGGGCCTAGCAAAAGCAGGGGATAATTCTA

TTAACCATGAAGACTATCATTGCTTTGAGCTACATTCTATGTCTGGTTT

TCGCTCAAAAACTTCCTGGAAATGACAATAGCACTGCAACGCTGTGC

CTTGGGCACCATGCAGTACCAAACGGAACGATAGTGAAAACAATCAC

GAATGACCAAATTGAAGTTACTAATGCTACTGAGCTGGTTCAGAATTC

CTCAATAGGTGAAATATGCGACAGTCCTCATCAGATCCTTGATGGAG

AAAACTGCACACTAATAGATGCTCTATTGGGAGACCCTCAGTGTGAT

GGCTTTCAAAATAAGAAATGGGACCTTTTTGTTGAACGAAGCAAAGC

CTACAGCAACTGTTACCCTTATGATGTGCCGGATTATGCCTCCCTTAG

GTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGAAAGCT

TCAATTGGGCTGGAGTCACTCAAAACGGAACAAGTTCTGCTTGCATA

AGGGGATCTAATAGTAGTTTCTTTAGTAGATTAAATTGGTTGACCCAC

TTAAACTTCAAGTACCCAGCATTGAACGTGACTATGCCAAACAATGAA

CAATTTGACAAATTGTACATTTGGGGGGTTCACCACCCGGGTACGGA

CAAGGACCAAATCTTCCTGTATGCTCAATCATCAGGAAGAATCACAGT

ATCTACCAAAAGAAGCCAACAAGCTGTAATCCCGAATATCGGATCTA

GACCCAGAATAAGGAATATCCCTAGCAGAATAAGCATCTATTGGACA

ATAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAGGGAATCTA

ATTGCTCCTAGGGGTTACTTCAAAATACGAAGTGGGAAAAGCTCAAT

AATGAGATCAGATGCACCCATTGGCAAATGCAAGTCTGAATGCATCA

CTCCAAATGGAAGCATTCCCAATGACAAACCATTCCAAAATGTAAACA

GGATCACATACGGGGCCTGTCCCAGATATGTTAAGCAAAGCACTCTG

AAATTGGCAACAGGAATGCGAAATGTACCAGAGAAACAAACTAGAGG

CATATTTGGCGCAATAGCGGGTTTCATAGAAAATGGTTGGGAGGGAA

TGGTGGATGGTTGGTACGGTTTCAGGCATCAAAATTCTGAGGGAAGA

GGACAAGCAGCAGATCTCAAAAGCACTCAAGCAGCAATCGATCAAAT

CAATGGGAAGCTGAATCGATTGATCGGGAAAACCAACGAGAAATTCC

ATCAGATTGAGAAAGAATTCTCAGAAGTAGAAGGGAGAATTCAGGAC

CTTGAGAAATATGTTGAGGACACAAAAATAGATCTCTGGTCATACAAC

GCAGAGCTTCTTGTTGCCCTGGAAAACCAACATACAATTGATCTAACT

GACTCAGAAATGAACAAACTGTTTGAAAAAACAAAGAAGCAACTGAG

GGAAAATGCTGAGGATATGGGCAATGGTTGTTTCAAAATATACCACA

AATGTGACAATGCCTGCATAGGATCAATCAGAAATGGAACTTATGAC

CACGATGTATACAGGGATGAAGCATTAAACAACCGGTTCCAGATCAA

GGGAGTTGAGCTGAAGTCAGGGTACAAAGATTGGATCCTATGGATTT

CCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCAT

CATGTGGGCCTGCCAAAAGGGCAACATTAGGTGCAACATTTGCATTT

GAGTGCATTAATTAAAAACACCCTTGTTTCTACTGGAAGCGGAGCCA

CGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAAAACCCC

GGGCCTATTTTCTAATATCCACAAAATGAAGGCAATAATTGTACTACT

CATGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACAT

CGTCAAACTCACCACATGTCGTCAAAACTGCTACTCAAGGGGAGGTC

AACGTGACCGGTGTAATACCACTGACAACAACACCCACCAAATCTCA

TTTTGCAAATCTCAAAGGAACAGAAACCAGGGGGAAACTATGCCCAA

AATGCCTCAACTGCACAGATCTGGATGTAGCCTTGGGCAGACCAAAA

TGCACAGGGAAAATACCCTCTGCAAGGGTTTCAATACTCCATGAAGT

CAGACCTGTTACATCTGGGTGCTTTCCTATAATGCACGATAGAACAAA

AATTAGACAGCTGCCTAACCTTCTCCGAGGATACGAACATGTCAGGT

TATCAACTCACAACGTTATCAATGCAAAAGATGCACCAGGAAGACCC

TACGAAATTGGAACCTCAGGGTCTTGCCCTAACATTACCAATGGAAA

CGGATTCTTCGCAACAATGGCTTGGGCCGTCCCAAAAAACAAAACAG

CAACAAATCCATTAACAATAGAAGTACCATACATTTGTACAGAAGGAG

AAGACCAAATTACCGTTTGGGGGTTCCACTCTGACAACGAGACCCAA

ATGGCAAAGCTCTATGGGGACTCAAAGCCCCAGAAGTTCACCTCATC

TGCCAACGGAGTGACCACACATTACGTTTCACAGATTGGTGGCTTCC

CAAATCAAACAGAAGACGGAGGACTACCACAAAGTGGCAGAATTGTT

GTTGATTACATGGTGCAGAAATCTGGAAAAACAGGAACAATTACCTAT

CAAAGAGGTATTTTATTGCCTCAAAAAGTGTGGTGCGCAAGTGGCAG

GAGCAAGGTAATAAAAGGATCCTTGCCCTTAATTGGAGAAGCAGATT

GCCTCCATGAAAAATACGGTGGATTAAACAAAAGCAAGCCTTACTAC

ACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGGTGAA

AACACCCTTGAAGCTGGCCAATGGAACCAAATATAGACCCCCTGCAA

AACTATTAAAGGAAAGAGGTTTCTTCGGAGCCATTGCTGGTTTCTTAG

AGGGAGGATGGGAAGGAATGATTGCAGGTTGGCACGGATACACATC

CCATGGGGCACATGGAGTAGCGGTGGCAGCTGACCTTAAGAGCACT

CAAGAGGCCATAAACAAGATAACAAAAAATCTCAACTCTTTGAGTGAG

CTGGAAGTAAAGAATCTTCAAAGACTAAGCGGTGCCATGGATGAACT

CCACAACGAAATACTAGAACTAGATGAGAAAGTGGATGATCTCAGAG

CTGACACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTTCCAATG

AAGGAATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGA

AAGCTGAAGAAAATGCTGGGCCCCTCTGCTGTAGAGATAGGGAATG

GATGCTTTGAAACCAAACACAAGTGCAACCAGACCTGCCTCGACAGA

ATAGCTGCTGGTACCTTTGATGCAGGAGAATTTTCTCTCCCCACCTTT

GATTCACTGAATATTACTGCTGCATCTTTAAATGACGACGGATTGGAC

AATCATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCT

GTAACACTGATGATAGCTATCTTTGTTGTTTATATGGTCTCCAGAGAC

AATGTTTCTTGCTCCATTTGTCTATAAGGAAAGTTAAGCCCTGTATTTT

CCTTTATTGTAGTGCTTGTTTGCTTGTTGTCATTACAAAGAAACGTTAT

TGAAAAATTGATGA

Pan-CoV-Flu
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCG
416

Vaccine #28
TGAACCTGATCACCAGGACCCAGAGCTACACCAACAGCTTCACCAGG

CoV-Omicron
GGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGCGTGCTGCACA

BA.2 Spike(2
GCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGTGACCTGGTTC

proline
CACGCCATCCACGTGAGCGGCACCAACGGCACCAAGAGGTTCGACA

substitution)-
ACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCGCCAGCACCGA

Influenza (HA)
GAAGAGCAACATCATCAGGGGCTGGATCTTCGGCACCACCCTGGAC

AGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCCACCAACGTGG

TGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCCCTTCCTGGAC

GTGTACTACCACAAGAACAACAAGAGCTGGATGGAGAGCGAGTTCA

GGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTACGTGAGCCA

GCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAACTTCAAGAAC

CTGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTTCAAGATCTA

CAGCAAGCACACCCCCATCAACCTGGGCAGGGACCTGCCCCAGGGC

TTCAGCGCCCTGGAGCCCCTGGTGGACCTGCCCATCGGCATCAACA

TCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAGCTACCTGAC

CCCCGGCGACAGCAGCAGCGGCTGGACCGCCGGCGCCGCCGCCTA

CTACGTGGGCTACCTGCAGCCCAGGACCTTCCTGCTGAAGTACAAC

GAGAACGGCACCATCACCGACGCCGTGGACTGCGCCCTGGACCCC

CTGAGCGAGACCAAGTGCACCCTGAAGAGCTTCACCGTGGAGAAGG

GCATCTACCAGACCAGCAACTTCAGGGTGCAGCCCACCGAGAGCAT

CGTGAGGTTCCCCAACATCACCAACCTGTGCCCCTTCGACGAGGTGT

TCAACGCCACCAGGTTCGCCAGCGTGTACGCCTGGAACAGGAAGAG

GATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTACAACTTCGCC

CCCTTCTTCGCCTTCAAGTGCTACGGCGTGAGCCCCACCAAGCTGAA

CGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTCGTGATCAGG

GGCAACGAGGTGAGCCAGATCGCCCCCGGCCAGACCGGCAACATC

GCCGACTACAACTACAAGCTGCCCGACGACTTCACCGGCTGCGTGA

TCGCCTGGAACAGCAACAAGCTGGACAGCAAGGTGGGCGGCAACTA

CAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGAAGCCCTTCG

AGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAACAAGCCCTG

CAACGGCGTGGCCGGCTTCAACTGCTACTTCCCCCTGAGGAGCTAC

GGCTTCAGGCCCACCTACGGCGTGGGCCACCAGCCCTACAGGGTG

GTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCACCGTGTGCG

GCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGCGTGAACTT

CAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCGAGAGCAAC

AAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACATCGCCGACA

CCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGATCCTGGACAT

CACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACCCCCGGCACC

AACACCAGCAACCAGGTGGCCGTGCTGTACCAGGGCGTGAACTGCA

CCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGACCCCCACCTG

GAGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACCAGGGCCGG

CTGCCTGATCGGCGCCGAGTACGTGAACAACAGCTACGAGTGCGAC

ATCCCCATCGGCGCCGGCATCTGCGCCAGCTACCAGACCCAGACCA

AGAGCCACCAGCAGGCCCAGAGCGTGGCCAGCCAGAGCATCATCG

CCTACACCATGAGCCTGGGCGCCGAGAACAGCGTGGCCTACAGCAA

CAACAGCATCGCCATCCCCACCAACTTCACCATCAGCGTGACCACCG

AGATCCTGCCCGTGAGCATGACCAAGACCAGCGTGGACTGCACCAT

GTACATCTGCGGCGACAGCACCGAGTGCAGCAACCTGCTGCTGCAG

TACGGCAGCTTCTGCACCCAGCTGAAGAGGGCCCTGACCGGCATCG

CCGTGGAGCAGGACAAGAACACCCAGGAGGTGTTCGCCCAGGTGAA

GCAGATCTACAAGACCCCCCCCATCAAGTACTTCGGCGGCTTCAACT

TCAGCCAGATCCTGCCCGACCCCAGCAAGCCCAGCAAGAGGAGCTT

CATCGAGGACCTGCTGTTCAACAAGGTGACCCTGGCCGACGCCGGC

TTCATCAAGCAGTACGGCGACTGCCTGGGCGACATCGCCGCCAGGG

ACCTGATCTGCGCCCAGAAGTTCAACGGCCTGACCGTGCTGCCCCC

CCTGCTGACCGACGAGATGATCGCCCAGTACACCAGCGCCCTGCTG

GCCGGCACCATCACCAGCGGCTGGACCTTCGGCGCCGGCGCCGCC

CTGCAGATCCCCTTCGCCATGCAGATGGCCTACAGGTTCAACGGCAT

CGGCGTGACCCAGAACGTGCTGTACGAGAACCAGAAGCTGATCGCC

AACCAGTTCAACAGCGCCATCGGCAAGATCCAGGACAGCCTGAGCA

GCACCGCCAGCGCCCTGGGCAAGCTGCAGGACGTGGTGAACCACA

ACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTGAGCAGCAAGTT

CGGCGCCATCAGCAGCGTGCTGAACGACATCCTGAGCAGGCTGGAC

CCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGATCACCGGCAGG

CTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCTGATCAGGGCCG

CCGAGATCAGGGCCAGCGCCAACCTGGCCGCCACCAAGATGAGCG

AGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTCTGCGGCAAGG

GCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCCACGGCGTGGT

GTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAAGAACTTCACC

ACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCACTTCCCCAGGG

AGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCGTGACCCAGAG

GAACTTCTACGAGCCCCAGATCATCACCACCGACAACACCTTCGTGA

GCGGCAACTGCGACGTGGTGATCGGCATCGTGAACAACACCGTGTA

CGACCCCCTGCAGCCCGAGCTGGACAGCTTCAAGGAGGAGCTGGAC

AAGTACTTCAAGAACCACACCAGCCCCGACGTGGACCTGGGCGACA

TCAGCGGCATCAACGCCAGCGTGGTGAACATCCAGAAGGAGATCGA

CAGGCTGAACGAGGTGGCCAAGAACCTGAACGAGAGCCTGATCGAC

CTGCAGGAGCTGGGCAAGTACGAGCAGTACATCAAGTGGCCCTGGT

ACATCTGGCTGGGCTTCATCGCCGGCCTGATCGCCATCGTGATGGT

GACCATCATGCTGTGCTGCATGACCAGCTGCTGCAGCTGCCTGAAG

GGCTGCTGCAGCTGCGGCAGCTGCTGCAAGTTCGACGAGGACGACA

GCGAGCCCGTGCTGAAGGGCGTGAAGCTGCACTACACCGGAAGCG

GAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGAA

AACCCCGGGCCTATGAAGGCAATACTAGTAGTTCTGCTATATACATTT

GCAACCGCAAATGCAGACACATTATGTATAGGTTATCATGCGAACAA

CTCAACAGACACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAA

CACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGC

AAACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGC

TGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCTCCACAGCAA

GCTCATGGTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACG

TGTTACCCAGGAGATTTCATCGATTATGAGGAGCTAAGAGAGCAATT

GAGCTCAGTGTCATCATTTGAAAGGTTTGAGATATTCCCCAAGACAA

GTTCATGGCCCAATCATGACTCGAACAAAGGTGTAACGACAGCATGT

CCTCATGCTGGAGCAAAAAGCTTCTACAAAAATTTAATATGGCTAGTT

AAAAAAGGAAATTCATACCCAAAGCTCAGCAAATCCTACATTAATGAT

AAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTCACCATCCACCTAC

TAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTTT

TGTGGGGACATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAA

TAAGGCCCAAAGTGAGGGATCAAGAAGGGAGAATGAACTATTACTGG

ACACTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAA

TCTAGTGGTACCGAGATATGCATTCGCAATGGAAAGAAATGCTGGAT

CTGGTATTATCATTTCAGATACACCAGTCCACGATTGCAATACAACTT

GTCAGACACCCAAGGGTGCTATAAACACCAGCCTCCCATTTCAGAAC

ATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGCACA

AAATTGAGACTGGCCACAGGATTGAGGAATGTCCCGTCCATTCAATC

TAGAGGCCTATTTGGGGCCATTGCCGGTTTCATTGAAGGGGGGTGG

ACAGGGATGGTAGATGGATGGTACGGTTATCACCATCAAAATGAGCA

GGGGTCAGGATATGCAGCCGACCTGAAGAGCACACAGAATGCCATT

GACGAGATTACTAACAAAGTAAACTCTGTTATTGAAAAAATGAATACA

CAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAAT

AGAGAATTTAAATAAAAAAGTTGATGATGGTTTCCTGGACATTTGGAC

TTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGA

CTACCACGATTCAAAGGTGAAGAACTTATATGAAAAGGTAAGAAGCC

AGTTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTT

ACCACAAATGTGATAACACGTGCATGGAAAGTGTCAAAAATGGGACT

TATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAA

ATAGATGGGGTAAAGCTGGAATCAACAAGGATTTACCAGATTTTGGC

GATCTATTCAACCGTCGCCAGTTCATTGGTACTGGTAGTCTCCCTGG

GGGCAATCAGTTTCTGGATGTGCTCTAATGGGTCTCTACAGTGTAGA

ATATGTATTTAAGGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAA

GCAGGAGATGTTGAAGAAAACCCCGGGCCTAGCAAAAGCAGGGGAT

AATTCTATTAACCATGAAGACTATCATTGCTTTGAGCTACATTCTATGT

CTGGTTTTCGCTCAAAAACTTCCTGGAAATGACAATAGCACTGCAAC

GCTGTGCCTTGGGCACCATGCAGTACCAAACGGAACGATAGTGAAAA

CAATCACGAATGACCAAATTGAAGTTACTAATGCTACTGAGCTGGTTC

AGAATTCCTCAATAGGTGAAATATGCGACAGTCCTCATCAGATCCTTG

ATGGAGAAAACTGCACACTAATAGATGCTCTATTGGGAGACCCTCAG

TGTGATGGCTTTCAAAATAAGAAATGGGACCTTTTTGTTGAACGAAGC

AAAGCCTACAGCAACTGTTACCCTTATGATGTGCCGGATTATGCCTC

CCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG

AAAGCTTCAATTGGGCTGGAGTCACTCAAAACGGAACAAGTTCTGCT

TGCATAAGGGGATCTAATAGTAGTTTCTTTAGTAGATTAAATTGGTTG

ACCCACTTAAACTTCAAGTACCCAGCATTGAACGTGACTATGCCAAAC

AATGAACAATTTGACAAATTGTACATTTGGGGGGTTCACCACCCGGG

TACGGACAAGGACCAAATCTTCCTGTATGCTCAATCATCAGGAAGAA

TCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCGAATATC

GGATCTAGACCCAGAATAAGGAATATCCCTAGCAGAATAAGCATCTA

TTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAG

GGAATCTAATTGCTCCTAGGGGTTACTTCAAAATACGAAGTGGGAAA

AGCTCAATAATGAGATCAGATGCACCCATTGGCAAATGCAAGTCTGA

ATGCATCACTCCAAATGGAAGCATTCCCAATGACAAACCATTCCAAAA

TGTAAACAGGATCACATACGGGGCCTGTCCCAGATATGTTAAGCAAA

GCACTCTGAAATTGGCAACAGGAATGCGAAATGTACCAGAGAAACAA

ACTAGAGGCATATTTGGCGCAATAGCGGGTTTCATAGAAAATGGTTG

GGAGGGAATGGTGGATGGTTGGTACGGTTTCAGGCATCAAAATTCTG

AGGGAAGAGGACAAGCAGCAGATCTCAAAAGCACTCAAGCAGCAAT

CGATCAAATCAATGGGAAGCTGAATCGATTGATCGGGAAAACCAACG

AGAAATTCCATCAGATTGAGAAAGAATTCTCAGAAGTAGAAGGGAGA

ATTCAGGACCTTGAGAAATATGTTGAGGACACAAAAATAGATCTCTGG

TCATACAACGCAGAGCTTCTTGTTGCCCTGGAAAACCAACATACAATT

GATCTAACTGACTCAGAAATGAACAAACTGTTTGAAAAAACAAAGAAG

CAACTGAGGGAAAATGCTGAGGATATGGGCAATGGTTGTTTCAAAAT

ATACCACAAATGTGACAATGCCTGCATAGGATCAATCAGAAATGGAA

CTTATGACCACGATGTATACAGGGATGAAGCATTAAACAACCGGTTC

CAGATCAAGGGAGTTGAGCTGAAGTCAGGGTACAAAGATTGGATCCT

ATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTG

GGGTTCATCATGTGGGCCTGCCAAAAGGGCAACATTAGGTGCAACAT

TTGCATTTGAGTGCATTAATTAAAAACACCCTTGTTTCTACTGGAAGC

GGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGTTGAAGA

AAACCCCGGGCCTATTTTCTAATATCCACAAAATGAAGGCAATAATTG

TACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGG

ATAACATCGTCAAACTCACCACATGTCGTCAAAACTGCTACTCAAGG

GGAGGTCAACGTGACCGGTGTAATACCACTGACAACAACACCCACCA

AATCTCATTTTGCAAATCTCAAAGGAACAGAAACCAGGGGGAAACTAT

GCCCAAAATGCCTCAACTGCACAGATCTGGATGTAGCCTTGGGCAGA

CCAAAATGCACAGGGAAAATACCCTCTGCAAGGGTTTCAATACTCCA

TGAAGTCAGACCTGTTACATCTGGGTGCTTTCCTATAATGCACGATAG

AACAAAAATTAGACAGCTGCCTAACCTTCTCCGAGGATACGAACATG

TCAGGTTATCAACTCACAACGTTATCAATGCAAAAGATGCACCAGGAA

GACCCTACGAAATTGGAACCTCAGGGTCTTGCCCTAACATTACCAAT

GGAAACGGATTCTTCGCAACAATGGCTTGGGCCGTCCCAAAAAACAA

AACAGCAACAAATCCATTAACAATAGAAGTACCATACATTTGTACAGA

AGGAGAAGACCAAATTACCGTTTGGGGGTTCCACTCTGACAACGAGA

CCCAAATGGCAAAGCTCTATGGGGACTCAAAGCCCCAGAAGTTCACC

TCATCTGCCAACGGAGTGACCACACATTACGTTTCACAGATTGGTGG

CTTCCCAAATCAAACAGAAGACGGAGGACTACCACAAAGTGGCAGAA

TTGTTGTTGATTACATGGTGCAGAAATCTGGAAAAACAGGAACAATTA

CCTATCAAAGAGGTATTTTATTGCCTCAAAAAGTGTGGTGCGCAAGT

GGCAGGAGCAAGGTAATAAAAGGATCCTTGCCCTTAATTGGAGAAGC

AGATTGCCTCCATGAAAAATACGGTGGATTAAACAAAAGCAAGCCTTA

CTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG

TGAAAACACCCTTGAAGCTGGCCAATGGAACCAAATATAGACCCCCT

GCAAAACTATTAAAGGAAAGAGGTTTCTTCGGAGCCATTGCTGGTTT

CTTAGAGGGAGGATGGGAAGGAATGATTGCAGGTTGGCACGGATAC

ACATCCCATGGGGCACATGGAGTAGCGGTGGCAGCTGACCTTAAGA

GCACTCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACTCTTTGA

GTGAGCTGGAAGTAAAGAATCTTCAAAGACTAAGCGGTGCCATGGAT

GAACTCCACAACGAAATACTAGAACTAGATGAGAAAGTGGATGATCT

CAGAGCTGACACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT

CCAATGAAGGAATAATAAACAGTGAAGATGAACATCTCTTGGCGCTT

GAAAGAAAGCTGAAGAAAATGCTGGGCCCCTCTGCTGTAGAGATAG

GGAATGGATGCTTTGAAACCAAACACAAGTGCAACCAGACCTGCCTC

GACAGAATAGCTGCTGGTACCTTTGATGCAGGAGAATTTTCTCTCCC

CACCTTTGATTCACTGAATATTACTGCTGCATCTTTAAATGACGACGG

ATTGGACAATCATACTATACTGCTTTACTACTCAACTGCTGCCTCCAG

TTTGGCTGTAACACTGATGATAGCTATCTTTGTTGTTTATATGGTCTC

CAGAGACAATGTTTCTTGCTCCATTTGTCTATAAGGAAAGTTAAGCCC

TGTATTTTCCTTTATTGTAGTGCTTGTTTGCTTGTTGTCATTACAAAGA

AACGTTATTGAAAAATTGATGA

Pan-CoV-Flu
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCG
417

Vaccine #29
TGAACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAG

CoV-Wuhan
CTTCACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGC

Spike(HexaPro)-
GTGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGT

Influenza (HA)
GACCTGGTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAG

AGGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCG

CCAGCACCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCAC

CACCCTGGACAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCC

ACCAACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCC

CTTCCTGGGCGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGA

GCGAGTTCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTA

CGTGAGCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAAC

TTCAAGAACCTGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTT

CAAGATCTACAGCAAGCACACCCCCATCAACCTGGTGAGGGACCTG

CCCCAGGGCTTCAGCGCCCTGGAGCCCCTGGTGGACCTGCCCATCG

GCATCAACATCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAG

CTACCTGACCCCCGGCGACAGCAGCAGCGGCTGGACCGCCGGCGC

CGCCGCCTACTACGTGGGCTACCTGCAGCCCAGGACCTTCCTGCTG

AAGTACAACGAGAACGGCACCATCACCGACGCCGTGGACTGCGCCC

TGGACCCCCTGAGCGAGACCAAGTGCACCCTGAAGAGCTTCACCGT

GGAGAAGGGCATCTACCAGACCAGCAACTTCAGGGTGCAGCCCACC

GAGAGCATCGTGAGGTTCCCCAACATCACCAACCTGTGCCCCTTCG

GCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGTACGCCTGGAA

CAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTAC

AACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTGAGCCCCA

CCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTC

GTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCAGACC

GGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCACCG

GCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGGG

CGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAG

CACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTG

CAGAGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCT

ACAGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCAC

CGTGTGCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGC

GTGAACTTCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCG

AGAGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACAT

CGCCGACACCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGAT

CCTGGACATCACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACC

CCCGGCACCAACACCAGCAACCAGGTGGCCGTGCTGTACCAGGACG

TGAACTGCACCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGAC

CCCCACCTGGAGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACC

AGGGCCGGCTGCCTGATCGGCGCCGAGCACGTGAACAACAGCTAC

GAGTGCGACATCCCCATCGGCGCCGGCATCTGCGCCAGCTACCAGA

CCCAGACCAACAGCCCCGGCAGCGCCAGCAGCGTGGCCAGCCAGA

GCATCATCGCCTACACCATGAGCCTGGGCGCCGAGAACAGCGTGGC

CTACAGCAACAACAGCATCGCCATCCCCACCAACTTCACCATCAGCG

TGACCACCGAGATCCTGCCCGTGAGCATGACCAAGACCAGCGTGGA

CTGCACCATGTACATCTGCGGCGACAGCACCGAGTGCAGCAACCTG

CTGCTGCAGTACGGCAGCTTCTGCACCCAGCTGAACAGGGCCCTGA

CCGGCATCGCCGTGGAGCAGGACAAGAACACCCAGGAGGTGTTCGC

CCAGGTGAAGCAGATCTACAAGACCCCCCCCATCAAGGACTTCGGC

GGCTTCAACTTCAGCCAGATCCTGCCCGACCCCAGCAAGCCCAGCA

AGAGGAGCCCCATCGAGGACCTGCTGTTCAACAAGGTGACCCTGGC

CGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCTGGGCGACATC

GCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACGGCCTGACCG

TGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAGTACACCAG

CGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTTCGGCGC

CGGCCCCGCCCTGCAGATCCCCTTCCCCATGCAGATGGCCTACAGG

TTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACCAGA

AGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGGA

CAGCCTGAGCAGCACCCCCAGCGCCCTGGGCAAGCTGCAGGACGT

GGTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTG

AGCAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGA

GCAGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGA

TCACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCT

GATCAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCAC

CAAGATGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTC

TGCGGCAAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCC

ACGGCGTGGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAA

GAACTTCACCACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCAC

TTCCCCAGGGAGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCG

TGACCCAGAGGAACTTCTACGAGCCCCAGATCATCACCACCGACAAC

ACCTTCGTGAGCGGCAACTGCGACGTGGTGATCGGCATCGTGAACA

ACACCGTGTACGACCCCCTGCAGCCCGAGCTGGACAGCTTCAAGGA

GGAGCTGGACAAGTACTTCAAGAACCACACCAGCCCCGACGTGGAC

CTGGGCGACATCAGCGGCATCAACGCCAGCGTGGTGAACATCCAGA

AGGAGATCGACAGGCTGAACGAGGTGGCCAAGAACCTGAACGAGAG

CCTGATCGACCTGCAGGAGCTGGGCAAGTACGAGCAGTACATCAAG

TGGCCCTGGTACATCTGGCTGGGCTTCATCGCCGGCCTGATCGCCA

TCGTGATGGTGACCATCATGCTGTGCTGCATGACCAGCTGCTGCAGC

TGCCTGAAGGGCTGCTGCAGCTGCGGCAGCTGCTGCAAGTTCGACG

AGGACGACAGCGAGCCCGTGCTGAAGGGCGTGAAGCTGCACTACAC

CGGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGAT

GTTGAAGAAAACCCCGGGCCTATGAAGGCAATACTAGTAGTTCTGCT

ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCA

TGCGAACAACTCAACAGACACTGTAGACACAGTACTAGAAAAGAATG

TAACAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGA

AACTATGCAAACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGT

AACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCTC

CACAGCAAGCTCATGGTCCTACATTGTGGAAACATCTAGTTCAGACA

ATGGAACGTGTTACCCAGGAGATTTCATCGATTATGAGGAGCTAAGA

GAGCAATTGAGCTCAGTGTCATCATTTGAAAGGTTTGAGATATTCCCC

AAGACAAGTTCATGGCCCAATCATGACTCGAACAAAGGTGTAACGAC

AGCATGTCCTCATGCTGGAGCAAAAAGCTTCTACAAAAATTTAATATG

GCTAGTTAAAAAAGGAAATTCATACCCAAAGCTCAGCAAATCCTAGAT

TAATGATAAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTCACCATC

CACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCA

TATGTTTTTGTGGGGACATCAAGATACAGCAAGAAGTTCAAGCCGGA

AATAGCAATAAGGCCCAAAGTGAGGGATCAAGAAGGGAGAATGAACT

ATTACTGGACACTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCA

ACTGGAAATCTAGTGGTACCGAGATATGCATTCGCAATGGAAAGAAA

TGCTGGATCTGGTATTATCATTTCAGATACACCAGTCCACGATTGCAA

TACAACTTGTCAGACACCCAAGGGTGCTATAAACACCAGCCTCCCAT

TTCAGAACATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAA

AAAGCACAAAATTGAGACTGGCCACAGGATTGAGGAATGTCCCGTCC

ATTCAATCTAGAGGCCTATTTGGGGCCATTGCCGGTTTCATTGAAGG

GGGGTGGACAGGGATGGTAGATGGATGGTACGGTTATCACCATCAA

AATGAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACACAGA

ATGCCATTGACGAGATTACTAACAAAGTAAACTCTGTTATTGAAAAAA

TGAATACACAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAA

AAAAGAATAGAGAATTTAAATAAAAAAGTTGATGATGGTTTCCTGGAC

ATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGA

ACTTTGGACTACCACGATTCAAAGGTGAAGAACTTATATGAAAAGGTA

AGAAGCCAGTTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTT

TGAATTTTACCACAAATGTGATAACACGTGCATGGAAAGTGTCAAAAA

TGGGACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACA

GAGAAGAAATAGATGGGGTAAAGCTGGAATCAACAAGGATTTACCAG

ATTTTGGCGATCTATTCAACCGTCGCCAGTTCATTGGTACTGGTAGTC

TCCCTGGGGGCAATCAGTTTCTGGATGTGCTCTAATGGGTCTCTACA

GTGTAGAATATGTATTTAAGGAAGCGGAGCCACGAACTTCTCTCTGTT

AAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTAGCAAAAGC

AGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTTGAGCTACA

TTCTATGTCTGGTTTTCGCTCAAAAACTTCCTGGAAATGACAATAGCA

CTGCAACGCTGTGCCTTGGGCACCATGCAGTACCAAACGGAACGAT

AGTGAAAACAATCACGAATGACCAAATTGAAGTTACTAATGCTACTGA

GCTGGTTCAGAATTCCTCAATAGGTGAAATATGCGACAGTCCTCATC

AGATCCTTGATGGAGAAAACTGCACACTAATAGATGCTCTATTGGGA

GACCCTCAGTGTGATGGCTTTCAAAATAAGAAATGGGACCTTTTTGTT

GAACGAAGCAAAGCCTACAGCAACTGTTACCCTTATGATGTGCCGGA

TTATGCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGT

TTAACAATGAAAGCTTCAATTGGGCTGGAGTCACTCAAAACGGAACA

AGTTCTGCTTGCATAAGGGGATCTAATAGTAGTTTCTTTAGTAGATTA

AATTGGTTGACCCACTTAAACTTCAAGTACCCAGCATTGAACGTGACT

ATGCCAAACAATGAACAATTTGACAAATTGTACATTTGGGGGGTTCAC

CACCCGGGTACGGACAAGGACCAAATCTTCCTGTATGCTCAATCATC

AGGAAGAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCC

CGAATATCGGATCTAGACCCAGAATAAGGAATATCCCTAGCAGAATA

AGCATCTATTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAAC

AGCACAGGGAATCTAATTGCTCCTAGGGGTTACTTCAAAATACGAAG

TGGGAAAAGCTCAATAATGAGATCAGATGCACCCATTGGCAAATGCA

AGTCTGAATGCATCACTCCAAATGGAAGCATTCCCAATGACAAACCAT

TCCAAAATGTAAACAGGATCACATACGGGGCCTGTCCCAGATATGTT

AAGCAAAGCACTCTGAAATTGGCAACAGGAATGCGAAATGTACCAGA

GAAACAAACTAGAGGCATATTTGGCGCAATAGCGGGTTTCATAGAAA

ATGGTTGGGAGGGAATGGTGGATGGTTGGTACGGTTTCAGGCATCA

AAATTCTGAGGGAAGAGGACAAGCAGCAGATCTCAAAAGCACTCAAG

CAGCAATCGATCAAATCAATGGGAAGCTGAATCGATTGATCGGGAAA

ACCAACGAGAAATTCCATCAGATTGAGAAAGAATTCTCAGAAGTAGAA

GGGAGAATTCAGGACCTTGAGAAATATGTTGAGGACACAAAAATAGA

TCTCTGGTCATACAACGCAGAGCTTCTTGTTGCCCTGGAAAACCAAC

ATACAATTGATCTAACTGACTCAGAAATGAACAAACTGTTTGAAAAAA

CAAAGAAGCAACTGAGGGAAAATGCTGAGGATATGGGCAATGGTTGT

TTCAAAATATACCACAAATGTGACAATGCCTGCATAGGATCAATCAGA

AATGGAACTTATGACCACGATGTATACAGGGATGAAGCATTAAACAA

CCGGTTCCAGATCAAGGGAGTTGAGCTGAAGTCAGGGTACAAAGATT

GGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGC

TTTGTTGGGGTTCATCATGTGGGCCTGCCAAAAGGGCAACATTAGGT

GCAACATTTGCATTTGAGTGCATTAATTAAAAACACCCTTGTTTCTACT

GGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGT

TGAAGAAAACCCCGGGCCTATTTTCTAATATCCACAAAATGAAGGCAA

TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCA

CTGGGATAACATCGTCAAACTCACCACATGTCGTCAAAACTGCTACT

CAAGGGGAGGTCAACGTGACCGGTGTAATACCACTGACAACAACAC

CCACCAAATCTCATTTTGCAAATCTCAAAGGAACAGAAACCAGGGGG

AAACTATGCCCAAAATGCCTCAACTGCACAGATCTGGATGTAGCCTT

GGGCAGACCAAAATGCACAGGGAAAATACCCTCTGCAAGGGTTTCAA

TACTCCATGAAGTCAGACCTGTTACATCTGGGTGCTTTCCTATAATGC

ACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTCCGAGGATAC

GAACATGTCAGGTTATCAACTCACAACGTTATCAATGCAAAAGATGCA

CCAGGAAGACCCTACGAAATTGGAACCTCAGGGTCTTGCCCTAACAT

TACCAATGGAAACGGATTCTTCGCAACAATGGCTTGGGCCGTCCCAA

AAAACAAAACAGCAACAAATCCATTAACAATAGAAGTACCATACATTT

GTACAGAAGGAGAAGACCAAATTACCGTTTGGGGGTTCCACTCTGAC

AACGAGACCCAAATGGCAAAGCTCTATGGGGACTCAAAGCCCCAGA

AGTTCACCTCATCTGCCAACGGAGTGACCACACATTACGTTTCACAG

ATTGGTGGCTTCCCAAATCAAACAGAAGACGGAGGACTACCACAAAG

TGGCAGAATTGTTGTTGATTACATGGTGCAGAAATCTGGAAAAACAG

GAACAATTACCTATCAAAGAGGTATTTTATTGCCTCAAAAAGTGTGGT

GCGCAAGTGGCAGGAGCAAGGTAATAAAAGGATCCTTGCCCTTAATT

GGAGAAGCAGATTGCCTCCATGAAAAATACGGTGGATTAAACAAAAG

CAAGCCTTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCC

CAATATGGGTGAAAACACCCTTGAAGCTGGCCAATGGAACCAAATAT

AGACCCCCTGCAAAACTATTAAAGGAAAGAGGTTTCTTCGGAGCCAT

TGCTGGTTTCTTAGAGGGAGGATGGGAAGGAATGATTGCAGGTTGG

CACGGATACACATCCCATGGGGCACATGGAGTAGCGGTGGCAGCTG

ACCTTAAGAGCACTCAAGAGGCCATAAACAAGATAACAAAAAATCTCA

ACTCTTTGAGTGAGCTGGAAGTAAAGAATCTTCAAAGACTAAGCGGT

GCCATGGATGAACTCCACAACGAAATACTAGAACTAGATGAGAAAGT

GGATGATCTCAGAGCTGACACAATAAGCTCACAAATAGAACTCGCAG

TCCTGCTTTCCAATGAAGGAATAATAAACAGTGAAGATGAACATCTCT

TGGCGCTTGAAAGAAAGCTGAAGAAAATGCTGGGCCCCTCTGCTGTA

GAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCAGAC

CTGCCTCGACAGAATAGCTGCTGGTACCTTTGATGCAGGAGAATTTT

CTCTCCCCACCTTTGATTCACTGAATATTACTGCTGCATCTTTAAATG

ACGACGGATTGGACAATCATACTATACTGCTTTACTACTCAACTGCTG

CCTCCAGTTTGGCTGTAACACTGATGATAGCTATCTTTGTTGTTTATA

TGGTCTCCAGAGACAATGTTTCTTGCTCCATTTGTCTATAAGGAAAGT

TAAGCCCTGTATTTTCCTTTATTGTAGTGCTTGTTTGCTTGTTGTCATT

ACAAAGAAACGTTATTGAAAAATTGATGA

Pan-CoV-Flu
ATGTTCGTGTTCCTGGTGCTGCTGCCCCTGGTGAGCAGCCAGTGCG
418

Vaccine #30
TGAACCTGACCACCAGGACCCAGCTGCCCCCCGCCTACACCAACAG

CoV-Wuhan
CTTCACCAGGGGCGTGTACTACCCCGACAAGGTGTTCAGGAGCAGC

Spike(2 proline
GTGCTGCACAGCACCCAGGACCTGTTCCTGCCCTTCTTCAGCAACGT

substitution)-
GACCTGGTTCCACGCCATCCACGTGAGCGGCACCAACGGCACCAAG

Influenza (HA)
AGGTTCGACAACCCCGTGCTGCCCTTCAACGACGGCGTGTACTTCG

CCAGCACCGAGAAGAGCAACATCATCAGGGGCTGGATCTTCGGCAC

CACCCTGGACAGCAAGACCCAGAGCCTGCTGATCGTGAACAACGCC

ACCAACGTGGTGATCAAGGTGTGCGAGTTCCAGTTCTGCAACGACCC

CTTCCTGGGCGTGTACTACCACAAGAACAACAAGAGCTGGATGGAGA

GCGAGTTCAGGGTGTACAGCAGCGCCAACAACTGCACCTTCGAGTA

CGTGAGCCAGCCCTTCCTGATGGACCTGGAGGGCAAGCAGGGCAAC

TTCAAGAACCTGAGGGAGTTCGTGTTCAAGAACATCGACGGCTACTT

CAAGATCTACAGCAAGCACACCCCCATCAACCTGGTGAGGGACCTG

CCCCAGGGCTTCAGCGCCCTGGAGCCCCTGGTGGACCTGCCCATCG

GCATCAACATCACCAGGTTCCAGACCCTGCTGGCCCTGCACAGGAG

CTACCTGACCCCCGGCGACAGCAGCAGCGGCTGGACCGCCGGCGC

CGCCGCCTACTACGTGGGCTACCTGCAGCCCAGGACCTTCCTGCTG

AAGTACAACGAGAACGGCACCATCACCGACGCCGTGGACTGCGCCC

TGGACCCCCTGAGCGAGACCAAGTGCACCCTGAAGAGCTTCACCGT

GGAGAAGGGCATCTACCAGACCAGCAACTTCAGGGTGCAGCCCACC

GAGAGCATCGTGAGGTTCCCCAACATCACCAACCTGTGCCCCTTCG

GCGAGGTGTTCAACGCCACCAGGTTCGCCAGCGTGTACGCCTGGAA

CAGGAAGAGGATCAGCAACTGCGTGGCCGACTACAGCGTGCTGTAC

AACAGCGCCAGCTTCAGCACCTTCAAGTGCTACGGCGTGAGCCCCA

CCAAGCTGAACGACCTGTGCTTCACCAACGTGTACGCCGACAGCTTC

GTGATCAGGGGCGACGAGGTGAGGCAGATCGCCCCCGGCCAGACC

GGCAAGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCACCG

GCTGCGTGATCGCCTGGAACAGCAACAACCTGGACAGCAAGGTGGG

CGGCAACTACAACTACCTGTACAGGCTGTTCAGGAAGAGCAACCTGA

AGCCCTTCGAGAGGGACATCAGCACCGAGATCTACCAGGCCGGCAG

CACCCCCTGCAACGGCGTGGAGGGCTTCAACTGCTACTTCCCCCTG

CAGAGCTACGGCTTCCAGCCCACCAACGGCGTGGGCTACCAGCCCT

ACAGGGTGGTGGTGCTGAGCTTCGAGCTGCTGCACGCCCCCGCCAC

CGTGTGCGGCCCCAAGAAGAGCACCAACCTGGTGAAGAACAAGTGC

GTGAACTTCAACTTCAACGGCCTGACCGGCACCGGCGTGCTGACCG

AGAGCAACAAGAAGTTCCTGCCCTTCCAGCAGTTCGGCAGGGACAT

CGCCGACACCACCGACGCCGTGAGGGACCCCCAGACCCTGGAGAT

CCTGGACATCACCCCCTGCAGCTTCGGCGGCGTGAGCGTGATCACC

CCCGGCACCAACACCAGCAACCAGGTGGCCGTGCTGTACCAGGACG

TGAACTGCACCGAGGTGCCCGTGGCCATCCACGCCGACCAGCTGAC

CCCCACCTGGAGGGTGTACAGCACCGGCAGCAACGTGTTCCAGACC

AGGGCCGGCTGCCTGATCGGCGCCGAGCACGTGAACAACAGCTAC

GAGTGCGACATCCCCATCGGCGCCGGCATCTGCGCCAGCTACCAGA

CCCAGACCAACAGCCCCAGGAGGGCCAGGAGCGTGGCCAGCCAGA

GCATCATCGCCTACACCATGAGCCTGGGCGCCGAGAACAGCGTGGC

CTACAGCAACAACAGCATCGCCATCCCCACCAACTTCACCATCAGCG

TGACCACCGAGATCCTGCCCGTGAGCATGACCAAGACCAGCGTGGA

CTGCACCATGTACATCTGCGGCGACAGCACCGAGTGCAGCAACCTG

CTGCTGCAGTACGGCAGCTTCTGCACCCAGCTGAACAGGGCCCTGA

CCGGCATCGCCGTGGAGCAGGACAAGAACACCCAGGAGGTGTTCGC

CCAGGTGAAGCAGATCTACAAGACCCCCCCCATCAAGGACTTCGGC

GGCTTCAACTTCAGCCAGATCCTGCCCGACCCCAGCAAGCCCAGCA

AGAGGAGCTTCATCGAGGACCTGCTGTTCAACAAGGTGACCCTGGC

CGACGCCGGCTTCATCAAGCAGTACGGCGACTGCCTGGGCGACATC

GCCGCCAGGGACCTGATCTGCGCCCAGAAGTTCAACGGCCTGACCG

TGCTGCCCCCCCTGCTGACCGACGAGATGATCGCCCAGTACACCAG

CGCCCTGCTGGCCGGCACCATCACCAGCGGCTGGACCTTCGGCGC

CGGCGCCGCCCTGCAGATCCCCTTCGCCATGCAGATGGCCTACAGG

TTCAACGGCATCGGCGTGACCCAGAACGTGCTGTACGAGAACCAGA

AGCTGATCGCCAACCAGTTCAACAGCGCCATCGGCAAGATCCAGGA

CAGCCTGAGCAGCACCGCCAGCGCCCTGGGCAAGCTGCAGGACGT

GGTGAACCAGAACGCCCAGGCCCTGAACACCCTGGTGAAGCAGCTG

AGCAGCAACTTCGGCGCCATCAGCAGCGTGCTGAACGACATCCTGA

GCAGGCTGGACCCCCCCGAGGCCGAGGTGCAGATCGACAGGCTGA

TCACCGGCAGGCTGCAGAGCCTGCAGACCTACGTGACCCAGCAGCT

GATCAGGGCCGCCGAGATCAGGGCCAGCGCCAACCTGGCCGCCAC

CAAGATGAGCGAGTGCGTGCTGGGCCAGAGCAAGAGGGTGGACTTC

TGCGGCAAGGGCTACCACCTGATGAGCTTCCCCCAGAGCGCCCCCC

ACGGCGTGGTGTTCCTGCACGTGACCTACGTGCCCGCCCAGGAGAA

GAACTTCACCACCGCCCCCGCCATCTGCCACGACGGCAAGGCCCAC

TTCCCCAGGGAGGGCGTGTTCGTGAGCAACGGCACCCACTGGTTCG

TGACCCAGAGGAACTTCTACGAGCCCCAGATCATCACCACCGACAAC

ACCTTCGTGAGCGGCAACTGCGACGTGGTGATCGGCATCGTGAACA

ACACCGTGTACGACCCCCTGCAGCCCGAGCTGGACAGCTTCAAGGA

GGAGCTGGACAAGTACTTCAAGAACCACACCAGCCCCGACGTGGAC

CTGGGCGACATCAGCGGCATCAACGCCAGCGTGGTGAACATCCAGA

AGGAGATCGACAGGCTGAACGAGGTGGCCAAGAACCTGAACGAGAG

CCTGATCGACCTGCAGGAGCTGGGCAAGTACGAGCAGTACATCAAG

TGGCCCTGGTACATCTGGCTGGGCTTCATCGCCGGCCTGATCGCCA

TCGTGATGGTGACCATCATGCTGTGCTGCATGACCAGCTGCTGCAGC

TGCCTGAAGGGCTGCTGCAGCTGCGGCAGCTGCTGCAAGTTCGACG

AGGACGACAGCGAGCCCGTGCTGAAGGGCGTGAAGCTGCACTACAC

CGGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGAT

GTTGAAGAAAACCCCGGGCCTATGAAGGCAATACTAGTAGTTCTGCT

ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCA

TGCGAACAACTCAACAGACACTGTAGACACAGTACTAGAAAAGAATG

TAACAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGA

AACTATGCAAACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGT

AACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCTC

CACAGCAAGCTCATGGTCCTACATTGTGGAAACATCTAGTTCAGACA

ATGGAACGTGTTACCCAGGAGATTTCATCGATTATGAGGAGCTAAGA

GAGCAATTGAGCTCAGTGTCATCATTTGAAAGGTTTGAGATATTCCCC

AAGACAAGTTCATGGCCCAATCATGACTCGAACAAAGGTGTAACGAC

AGCATGTCCTCATGCTGGAGCAAAAAGCTTCTACAAAAATTTAATATG

GCTAGTTAAAAAAGGAAATTCATACCCAAAGCTCAGCAAATCCTACAT

TAATGATAAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTCACCATC

CACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCA

TATGTTTTTGTGGGGACATCAAGATACAGCAAGAAGTTCAAGCCGGA

AATAGCAATAAGGCCCAAAGTGAGGGATCAAGAAGGGAGAATGAACT

ATTACTGGACACTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCA

ACTGGAAATCTAGTGGTACCGAGATATGCATTCGCAATGGAAAGAAA

TGCTGGATCTGGTATTATCATTTCAGATACACCAGTCCACGATTGCAA

TACAACTTGTCAGACACCCAAGGGTGCTATAAACACCAGCCTCCCAT

TTCAGAACATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAA

AAAGCACAAAATTGAGACTGGCCACAGGATTGAGGAATGTCCCGTCC

ATTCAATCTAGAGGCCTATTTGGGGCCATTGCCGGTTTCATTGAAGG

GGGGTGGACAGGGATGGTAGATGGATGGTACGGTTATCACCATCAA

AATGAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACACAGA

ATGCCATTGACGAGATTACTAACAAAGTAAACTCTGTTATTGAAAAAA

TGAATACACAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAA

AAAAGAATAGAGAATTTAAATAAAAAAGTTGATGATGGTTTCCTGGAC

ATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGA

ACTTTGGACTACCACGATTCAAAGGTGAAGAACTTATATGAAAAGGTA

AGAAGCCAGTTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTT

TGAATTTTACCACAAATGTGATAACACGTGCATGGAAAGTGTCAAAAA

TGGGACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACA

GAGAAGAAATAGATGGGGTAAAGCTGGAATCAACAAGGATTTACCAG

ATTTTGGCGATCTATTCAACCGTCGCCAGTTCATTGGTACTGGTAGTC

TCCCTGGGGGCAATCAGTTTCTGGATGTGCTCTAATGGGTCTCTACA

GTGTAGAATATGTATTTAAGGAAGCGGAGCCACGAACTTCTCTCTGTT

AAAGCAAGCAGGAGATGTTGAAGAAAACCCCGGGCCTAGCAAAAGC

AGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTTGAGCTACA

TTCTATGTCTGGTTTTCGCTCAAAAACTTCCTGGAAATGACAATAGCA

CTGCAACGCTGTGCCTTGGGCACCATGCAGTACCAAACGGAACGAT

AGTGAAAACAATCACGAATGACCAAATTGAAGTTACTAATGCTACTGA

GCTGGTTCAGAATTCCTCAATAGGTGAAATATGCGACAGTCCTCATC

AGATCCTTGATGGAGAAAACTGCACACTAATAGATGCTCTATTGGGA

GACCCTCAGTGTGATGGCTTTCAAAATAAGAAATGGGACCTTTTTGTT

GAACGAAGCAAAGCCTACAGCAACTGTTACCCTTATGATGTGCCGGA

TTATGCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGT

TTAACAATGAAAGCTTCAATTGGGCTGGAGTCACTCAAAACGGAACA

AGTTCTGCTTGCATAAGGGGATCTAATAGTAGTTTCTTTAGTAGATTA

AATTGGTTGACCCACTTAAACTTCAAGTACCCAGCATTGAACGTGACT

ATGCCAAACAATGAACAATTTGACAAATTGTACATTTGGGGGGTTCAC

CACCCGGGTACGGACAAGGACCAAATCTTCCTGTATGCTCAATCATC

AGGAAGAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCC

CGAATATCGGATCTAGACCCAGAATAAGGAATATCCCTAGCAGAATA

AGCATCTATTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAAC

AGCACAGGGAATCTAATTGCTCCTAGGGGTTACTTCAAAATACGAAG

TGGGAAAAGCTCAATAATGAGATCAGATGCACCCATTGGCAAATGCA

AGTCTGAATGCATCACTCCAAATGGAAGCATTCCCAATGACAAACCAT

TCCAAAATGTAAACAGGATCACATACGGGGCCTGTCCCAGATATGTT

AAGCAAAGCACTCTGAAATTGGCAACAGGAATGCGAAATGTACCAGA

GAAACAAACTAGAGGCATATTTGGCGCAATAGCGGGTTTCATAGAAA

ATGGTTGGGAGGGAATGGTGGATGGTTGGTACGGTTTCAGGCATCA

AAATTCTGAGGGAAGAGGACAAGCAGCAGATCTCAAAAGCACTCAAG

CAGCAATCGATCAAATCAATGGGAAGCTGAATCGATTGATCGGGAAA

ACCAACGAGAAATTCCATCAGATTGAGAAAGAATTCTCAGAAGTAGAA

GGGAGAATTCAGGACCTTGAGAAATATGTTGAGGACACAAAAATAGA

TCTCTGGTCATACAACGCAGAGCTTCTTGTTGCCCTGGAAAACCAAC

ATACAATTGATCTAACTGACTCAGAAATGAACAAACTGTTTGAAAAAA

CAAAGAAGCAACTGAGGGAAAATGCTGAGGATATGGGCAATGGTTGT

TTCAAAATATACCACAAATGTGACAATGCCTGCATAGGATCAATCAGA

AATGGAACTTATGACCACGATGTATACAGGGATGAAGCATTAAACAA

CCGGTTCCAGATCAAGGGAGTTGAGCTGAAGTCAGGGTACAAAGATT

GGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGC

TTTGTTGGGGTTCATCATGTGGGCCTGCCAAAAGGGCAACATTAGGT

GCAACATTTGCATTTGAGTGCATTAATTAAAAACACCCTTGTTTCTACT

GGAAGCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGAGATGT

TGAAGAAAACCCCGGGCCTATTTTCTAATATCCACAAAATGAAGGCAA

TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCA

CTGGGATAACATCGTCAAACTCACCACATGTCGTCAAAACTGCTACT

CAAGGGGAGGTCAACGTGACCGGTGTAATACCACTGACAACAACAC

CCACCAAATCTCATTTTGCAAATCTCAAAGGAACAGAAACCAGGGGG

AAACTATGCCCAAAATGCCTCAACTGCACAGATCTGGATGTAGCCTT

GGGCAGACCAAAATGCACAGGGAAAATACCCTCTGCAAGGGTTTCAA

TACTCCATGAAGTCAGACCTGTTACATCTGGGTGCTTTCCTATAATGC

ACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTCCGAGGATAC

GAACATGTCAGGTTATCAACTCACAACGTTATCAATGCAAAAGATGCA

CCAGGAAGACCCTACGAAATTGGAACCTCAGGGTCTTGCCCTAACAT

TACCAATGGAAACGGATTCTTCGCAACAATGGCTTGGGCCGTCCCAA

AAAACAAAACAGCAACAAATCCATTAACAATAGAAGTACCATACATTT

GTACAGAAGGAGAAGACCAAATTACCGTTTGGGGGTTCCACTCTGAC

AACGAGACCCAAATGGCAAAGCTCTATGGGGACTCAAAGCCCCAGA

AGTTCACCTCATCTGCCAACGGAGTGACCACACATTACGTTTCACAG

ATTGGTGGCTTCCCAAATCAAACAGAAGACGGAGGACTACCACAAAG

TGGCAGAATTGTTGTTGATTACATGGTGCAGAAATCTGGAAAAACAG

GAACAATTACCTATCAAAGAGGTATTTTATTGCCTCAAAAAGTGTGGT

GCGCAAGTGGCAGGAGCAAGGTAATAAAAGGATCCTTGCCCTTAATT

GGAGAAGCAGATTGCCTCCATGAAAAATACGGTGGATTAAACAAAAG

CAAGCCTTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCC

CAATATGGGTGAAAACACCCTTGAAGCTGGCCAATGGAACCAAATAT

AGACCCCCTGCAAAACTATTAAAGGAAAGAGGTTTCTTCGGAGCCAT

TGCTGGTTTCTTAGAGGGAGGATGGGAAGGAATGATTGCAGGTTGG

CACGGATACACATCCCATGGGGCACATGGAGTAGCGGTGGCAGCTG

ACCTTAAGAGCACTCAAGAGGCCATAAACAAGATAACAAAAAATCTCA

ACTCTTTGAGTGAGCTGGAAGTAAAGAATCTTCAAAGACTAAGCGGT

GCCATGGATGAACTCCACAACGAAATACTAGAACTAGATGAGAAAGT

GGATGATCTCAGAGCTGACACAATAAGCTCACAAATAGAACTCGCAG

TCCTGCTTTCCAATGAAGGAATAATAAACAGTGAAGATGAACATCTCT

TGGCGCTTGAAAGAAAGCTGAAGAAAATGCTGGGCCCCTCTGCTGTA

GAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCAGAC

CTGCCTCGACAGAATAGCTGCTGGTACCTTTGATGCAGGAGAATTTT

CTCTCCCCACCTTTGATTCACTGAATATTACTGCTGCATCTTTAAATG

ACGACGGATTGGACAATCATACTATACTGCTTTACTACTCAACTGCTG

CCTCCAGTTTGGCTGTAACACTGATGATAGCTATCTTTGTTGTTTATA

TGGTCTCCAGAGACAATGTTTCTTGCTCCATTTGTCTATAAGGAAAGT

TAAGCCCTGTATTTTCCTTTATTGTAGTGCTTGTTTGCTTGTTGTCATT

ACAAAGAAACGTTATTGAAAAATTGATGA

Pan-CoV-Flu
ATGATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCA
419

Vaccine #31
AATGCAGACACATTATGTATAGGTTATCATGCGAACAACTCAACAGAC

(H1N1)
ACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTT

AACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGAGG

GGTAGCCCCATTGCATTTGGGTAAATGTAACATTGCTGGCTGGATCC

TGGGAAATCCAGAGTGTGAATCACTCTCCACAGCAAGCTCATGGTCC

TACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAGG

AGATTTCATCGATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGT

CATCATTTGAAAGGTTTGAGATATTCCCCAAGACAAGTTCATGGCCCA

ATCATGACTCGAACAAAGGTGTAACGACAGCATGTCCTCATGCTGGA

GCAAAAAGCTTCTACAAAAATTTAATATGGCTAGTTAAAAAAGGAAAT

TCATACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGGGAAAGAA

GTCCTCGTGCTATGGGGCATTCACCATCCACCTACTAGTGCTGACCA

ACAAAGTCTCTATCAGAATGCAGATGCATATGTTTTTGTGGGGACATC

AAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGGCCCAAAG

TGAGGGATCAAGAAGGGAGAATGAACTATTACTGGACACTAGTAGAG

CCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGTGGTACC

GAGATATGCATTCGCAATGGAAAGAAATGCTGGATCTGGTATTATCAT

TTCAGATACACCAGTCCACGATTGCAATACAACTTGTCAGACACCCAA

GGGTGCTATAAACACCAGCCTCCCATTTCAGAACATACATCCGATCA

CAATTGGAAAATGTCCAAAATATGTAAAAAGCACAAAATTGAGACTGG

CCACAGGATTGAGGAATGTCCCGTCCATTCAATCTAGAGGCCTATTT

GGGGCCATTGCCGGTTTCATTGAAGGGGGGTGGACAGGGATGGTAG

ATGGATGGTACGGTTATCACCATCAAAATGAGCAGGGGTCAGGATAT

GCAGCCGACCTGAAGAGCACACAGAATGCCATTGACGAGATTACTAA

CAAAGTAAACTCTGTTATTGAAAAAATGAATACACAGTTCACAGCAGT

AGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGAATTTAAATAA

AAAAGTTGATGATGGTTTCCTGGACATTTGGACTTACAATGCCGAACT

GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAA

GGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGTTAAAAAACAATG

CCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACCACAAATGTGATA

ACACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAA

TACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGGTAAA

GCTGGAATCAACAAGGATTTACCAGATTTTGGCGATCTATTCAACCGT

CGCCAGTTCATTGGTACTGGTAGTCTCCCTGGGGGCAATCAGTTTCT

GGATGTGCTCTAATGGGTCTCTACAGTGTAGAATATGTATTTAATGAT

GA

Pan-CoV-Flu
ATGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTG
420

Vaccine #32
CTTTGAGCTACATTCTATGTCTGGTTTTCGCTCAAAAACTTCCTGGAA

(H3N2)
ATGACAATAGCACTGCAACGCTGTGCCTTGGGCACCATGCAGTACCA

AACGGAACGATAGTGAAAACAATCACGAATGACCAAATTGAAGTTACT

AATGCTACTGAGCTGGTTCAGAATTCCTCAATAGGTGAAATATGCGA

CAGTCCTCATCAGATCCTTGATGGAGAAAACTGCACACTAATAGATG

CTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAAGAAATGG

GACCTTTTTGTTGAACGAAGCAAAGCCTACAGCAACTGTTACCCTTAT

GATGTGCCGGATTATGCCTCCCTTAGGTCACTAGTTGCCTCATCCGG

CACACTGGAGTTTAACAATGAAAGCTTCAATTGGGCTGGAGTCACTC

AAAACGGAACAAGTTCTGCTTGCATAAGGGGATCTAATAGTAGTTTCT

TTAGTAGATTAAATTGGTTGACCCACTTAAACTTCAAGTACCCAGCAT

TGAACGTGACTATGCCAAACAATGAACAATTTGACAAATTGTACATTT

GGGGGGTTCACCACCCGGGTACGGACAAGGACCAAATCTTCCTGTA

TGCTCAATCATCAGGAAGAATCACAGTATCTACCAAAAGAAGCCAAC

AAGCTGTAATCCCGAATATCGGATCTAGACCCAGAATAAGGAATATC

CCTAGCAGAATAAGCATCTATTGGACAATAGTAAAACCGGGAGACAT

ACTTTTGATTAACAGCACAGGGAATCTAATTGCTCCTAGGGGTTACTT

CAAAATACGAAGTGGGAAAAGCTCAATAATGAGATCAGATGCACCCA

TTGGCAAATGCAAGTCTGAATGCATCACTCCAAATGGAAGCATTCCC

AATGACAAACCATTCCAAAATGTAAACAGGATCACATACGGGGCCTG

TCCCAGATATGTTAAGCAAAGCACTCTGAAATTGGCAACAGGAATGC

GAAATGTACCAGAGAAACAAACTAGAGGCATATTTGGCGCAATAGCG

GGTTTCATAGAAAATGGTTGGGAGGGAATGGTGGATGGTTGGTACG

GTTTCAGGCATCAAAATTCTGAGGGAAGAGGACAAGCAGCAGATCTC

AAAAGCACTCAAGCAGCAATCGATCAAATCAATGGGAAGCTGAATCG

ATTGATCGGGAAAACCAACGAGAAATTCCATCAGATTGAGAAAGAATT

CTCAGAAGTAGAAGGGAGAATTCAGGACCTTGAGAAATATGTTGAGG

ACACAAAAATAGATCTCTGGTCATACAACGCAGAGCTTCTTGTTGCCC

TGGAAAACCAACATACAATTGATCTAACTGACTCAGAAATGAACAAAC

TGTTTGAAAAAACAAAGAAGCAACTGAGGGAAAATGCTGAGGATATG

GGCAATGGTTGTTTCAAAATATACCACAAATGTGACAATGCCTGCATA

GGATCAATCAGAAATGGAACTTATGACCACGATGTATACAGGGATGA

AGCATTAAACAACCGGTTCCAGATCAAGGGAGTTGAGCTGAAGTCAG

GGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTT

GCTTTGTGTTGCTTTGTTGGGGTTCATCATGTGGGCCTGCCAAAAGG

GCAACATTAGGTGCAACATTTGCATTTGAGTGCATTAATTAAAAACAC

CCTTGTTTCTACTTGATGA

Pan-CoV-Flu
ATGATTTTCTAATATCCACAAAATGAAGGCAATAATTGTACTACTCATG
421

Vaccine #33
GTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACATCGTC

(H3N2)
AAACTCACCACATGTCGTCAAAACTGCTACTCAAGGGGAGGTCAACG

TGACCGGTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTG

CAAATCTCAAAGGAACAGAAACCAGGGGGAAACTATGCCCAAAATGC

CTCAACTGCACAGATCTGGATGTAGCCTTGGGCAGACCAAAATGCAC

AGGGAAAATACCCTCTGCAAGGGTTTCAATACTCCATGAAGTCAGAC

CTGTTACATCTGGGTGCTTTCCTATAATGCACGATAGAACAAAAATTA

GACAGCTGCCTAACCTTCTCCGAGGATACGAACATGTCAGGTTATCA

ACTCACAACGTTATCAATGCAAAAGATGCACCAGGAAGACCCTACGA

AATTGGAACCTCAGGGTCTTGCCCTAACATTACCAATGGAAACGGAT

TCTTCGCAACAATGGCTTGGGCCGTCCCAAAAAACAAAACAGCAACA

AATCCATTAACAATAGAAGTACCATACATTTGTACAGAAGGAGAAGAC

CAAATTACCGTTTGGGGGTTCCACTCTGACAACGAGACCCAAATGGC

AAAGCTCTATGGGGACTCAAAGCCCCAGAAGTTCACCTCATCTGCCA

ACGGAGTGACCACACATTACGTTTCACAGATTGGTGGCTTCCCAAAT

CAAACAGAAGACGGAGGACTACCACAAAGTGGCAGAATTGTTGTTGA

TTACATGGTGCAGAAATCTGGAAAAACAGGAACAATTACCTATCAAAG

AGGTATTTTATTGCCTCAAAAAGTGTGGTGCGCAAGTGGCAGGAGCA

AGGTAATAAAAGGATCCTTGCCCTTAATTGGAGAAGCAGATTGCCTC

CATGAAAAATACGGTGGATTAAACAAAAGCAAGCCTTACTACACAGG

GGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGGTGAAAACAC

CCTTGAAGCTGGCCAATGGAACCAAATATAGACCCCCTGCAAAACTA

TTAAAGGAAAGAGGTTTCTTCGGAGCCATTGCTGGTTTCTTAGAGGG

AGGATGGGAAGGAATGATTGCAGGTTGGCACGGATACACATCCCAT

GGGGCACATGGAGTAGCGGTGGCAGCTGACCTTAAGAGCACTCAAG

AGGCCATAAACAAGATAACAAAAAATCTCAACTCTTTGAGTGAGCTGG

AAGTAAAGAATCTTCAAAGACTAAGCGGTGCCATGGATGAACTCCAC

AACGAAATACTAGAACTAGATGAGAAAGTGGATGATCTCAGAGCTGA

CACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTTCCAATGAAG

GAATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAG

CTGAAGAAAATGCTGGGCCCCTCTGCTGTAGAGATAGGGAATGGAT

GCTTTGAAACCAAACACAAGTGCAACCAGACCTGCCTCGACAGAATA

GCTGCTGGTACCTTTGATGCAGGAGAATTTTCTCTCCCCACCTTTGAT

TCACTGAATATTACTGCTGCATCTTTAAATGACGACGGATTGGACAAT

CATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTA

ACACTGATGATAGCTATCTTTGTTGTTTATATGGTCTCCAGAGACAAT

GTTTCTTGCTCCATTTGTCTATAAGGAAAGTTAAGCCCTGTATTTTCCT

TTATTGTAGTGCTTGTTTGCTTGTTGTCATTACAAAGAAACGTTATTGA

AAAATTGATGA

In some embodiments, the recombinant vaccine compositions described herein are used to prevent a coronavirus and/or influenza disease in a subject. In other embodiments, the recombinant vaccine compositions described herein are used to prevent a coronavirus and/or influenza infection prophylactically in a subject. In further embodiments, the recombinant vaccine compositions described herein elicits an immune response in a subject.

Although there has been shown and described the preferred embodiment of the present invention, it will be readily apparent to those skilled in the art that modifications may be made thereto which do not exceed the scope of the appended claims. Therefore, the scope of the invention is only to be limited by the following claims. In some embodiments, the figures presented in this patent application are drawn to scale, including the angles, ratios of dimensions, etc. In some embodiments, the figures are representative only and the claims are not limited by the dimensions of the figures. In some embodiments, descriptions of the inventions described herein using the phrase “comprising” includes embodiments that could be described as “consisting essentially of” or “consisting of”, and as such the written description requirement for claiming one or more embodiments of the present invention using the phrase “consisting essentially of” or “consisting of” is met.

Number	Date	Country
63084421	Sep 2020	US
63009907	Apr 2020	US
63349799	Jun 2022	US
63349904	Jun 2022	US
63302454	Jan 2022	US

	Number	Date	Country
Parent	PCT/US21/27355	Apr 2021	US
Child	18046875		US

LARGE SEQUENCE PAN-CORONAVIRUS VACCINE COMPOSITIONS

Information

Publication Number

Date Filed

Date Published

Inventors

Original Assignees

CPC

International Classifications

Abstract

Description

Claims

CROSS-REFERENCES TO RELATED APPLICATIONS

STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT

Provisional Applications (5)

Continuation in Parts (1)