The present invention relates to a lens unit, an illumination cap member for use with the lens unit, a sample observation kit incorporated with the lens unit and the illumination cap member, and a transmission compound microscope. More specifically, the present invention relates to a technique for performing microscopic observation with use of a smart device having a camera function.
The Leeuwenhoek microscope invented by Leeuwenhoek in Holland more than about 350 years ago is the first-ever invented microscope in the world. The Leeuwenhoek microscope has a simple structure provided with a ball lens, and has been favorably used by the people for a long time, because of its high performance and high magnification (100 times to 300 times). The Applicants of the present application simplified the configuration of the Leeuwenhoek microscope, improved the operability, and proposed a single-lens microscope in which the performance was improved by using a high-performance ball lens (see Patent Document 1).
Meanwhile, the Leeuwenhoek microscope may constitute a lens compound microscope when the microscope is integrally used with the lens of an eye of an observer. In recent years, there is proposed a compound microscope, in which the lens of an eye of an observer is substituted by a photographing lens of a smart device having a camera function, such as a smartphone or a tablet terminal (see Non-Patent Documents 1 and 2).
It is reported that the compound microscopes of these new types can effectively use automatic focus or automatic exposure provided in a smart device as camera functions, and can obtain substantially the same performance as the performance of commercially available high-quality microscopes. For the aforementioned reasons, the spread of the Leeuwenhoek single-lens microscope incorporated with a smart device is expected in various fields such as education, medical treatment, healthcare industry, cosmetic industry, food industry, agriculture, forestry, and fishery industries, and entertainment.
The Leeuwenhoek single-lens microscope incorporated with a smart device as described above has been expected to rapidly spread by adding high functionality of the smart device. However, the Leeuwenhoek single-lens microscope incorporated with a smart device has not yet spread as expected, because of the following problems.
(1) The microscope is provided on the basis of the premises that the microscope is held by the user's hand. Therefore, a photographed image is likely to be blurred due to user's movement. This seems to be a disadvantage due to the fact that a smart device is a mobile device.
(2) As well as a camera, the microscope is a reflective microscope configured such that an object to be observed is observed by reflected light from the object surface, and is not a transmission microscope like a Leeuwenhoek microscope.
(3) A mechanism for fixing a Leeuwenhoek microscope to a smart device is necessary. This makes the mechanism complicated.
(4) When an object to be observed is a sample carried by the user's hand, a sample platform for fixing the sample is necessary. This makes the mechanism complicated.
(5) When an object to be observed is a fixed sample, illumination light may be blocked by a microscope body when the microscope body is moved closer to the sample. In view of the above, it is necessary to prepare an illumination device separately. This makes the mechanism complicated.
In view of the above, an object of the invention is to provide a lens unit, an illumination cap member, a sample observation kit, and a transmission compound microscope that enable to easily acquire a microscopic observation image by being mounted on a smart device with a simplified structure.
A lens unit according to an aspect of the invention is at least provided with a lens, and a holding member which holds the lens. The lens is disposed on an optical axis of a camera provided in a smart device. The lens and the camera constitute a transmission compound microscope.
The lens may be a ball lens or a GRIN lens.
Further, the holding member may be a transparent flat plate including a through-hole formed therein for accommodating the lens. In this configuration, an inner wall of the through-hole may be made opaque.
In the lens unit of the invention, at least an opening portion of the through-hole may be covered by a transparent resin sheet. In this configuration, preferably, a focus position of the lens may be on an outer surface of the transparent resin sheet.
Meanwhile, the lens and the holding member may be integrally formed.
The lens unit of the invention may be further provided with a slip stopper member made of resin or rubber. The slip stopper member may be formed on both or one of a smart-device-side surface of the lens unit, and a sample-side surface of the lens unit. In this configuration, the slip stopper member may include a hole formed therein for receiving at least a part of the lens.
An illumination cap member according to an aspect of the invention is an illumination cap member for use with the lens unit. The illumination cap member includes a cap portion made of an opaque material and configured to cover the lens, and a lighting lens placed on a top surface of the cap portion. A sample is disposed between the lens unit and the lighting lens.
An illumination cap member according to another aspect of the invention is an illumination cap member for use with the lens unit. The illumination cap member includes a cap portion made of an opaque material and configured to cover the lens, and a lighting diffusion plate placed on a top surface of the cap portion. A sample is disposed between the lens unit and the lighting diffusion plate.
Any one of the illumination cap members may further include a Fresnel lens which generates parallel light by using the lighting diffusion plate as a spot light source. In this configuration, a sample is disposed between the lens unit and the Fresnel lens.
The illumination cap member of the invention may further include a slip stopper member made of resin or rubber at a position in contact with the lens unit.
A sample observation kit according to an aspect of the invention includes the lens unit, and the illumination cap member.
The sample observation kit may further include a cover sheet made of a transparent resin and disposed on a sample.
The cover sheet may have such a shape that the cover sheet includes a middle portion of a shape that matches the illumination cap member, and a pair of handle portions formed on both ends of the middle portion.
In the aforementioned configuration, preferably, a lower portion of a side wall of the illumination cap member includes a cutaway at a position that matches the handle portion. The cutaway may have a depth equal to or larger than the thickness of the cover sheet.
A sample observation kit according to another aspect of the invention may be configured such that a through-hole is formed in each of the lens unit and the illumination cap member, and a string member is passed through each of the through-holes.
A microscope according to an aspect of the invention is provided with the lens unit or the sample observation kit; and a smart device having a camera function.
According to the invention, it is possible to implement a lens unit that enables to easily acquire a microscopic observation image by being mounted on a smart device with a simplified structure, a sample observation kit incorporated with the lens unit, and a transmission compound microscope incorporated with the lens unit.
In the following, embodiments of the invention are described in details referring to the accompanying drawings. It should be noted that the invention is not limited by the embodiments described below.
In the following, a lens unit in the first embodiment of the invention is described.
The lens 1 may be a ball lens or a GRIN lens made of glass or plastic. The size of the lens 1 is, for instance, from 1 to 5 mm. As the size of the lens decreases, the focal length decreases, and the magnification of a compound microscope integrally provided with a smart device increases. In view of the above, the size of the lens 1 is selected according to the magnification required by the user.
The holding member 3 may be a transparent flat plate or a translucent flat plate. The holding member 3 includes a through-hole 2 formed therein for accommodating the lens 1. In order to facilitate alignment between the optical axis of a camera of a smart device and the optical axis of the lens 1 of the lens unit 10, it is preferable to use a transparent material for the holding member 3. It is desirable, however, to make the holding member 3 opaque by e.g. applying black paint on the inner wall of the through-hole 2 or covering the inner wall of the through-hole 2 with a black sheet in order to prevent incidence of stray light to the lens 1.
Further, the holding member 3 may have a thickness such that the lens 1 does not protrude from the holding member 3 when the lens 1 is accommodated in the through-hole 2. Further, it is possible to color the periphery of the through-hole 2.
In the lens unit 10 in the embodiment, preferably, opening portions of the through-hole 2 of the holding member 3 may be respectively covered by a transparent resin sheet 4. The transparent resin sheet 4 may be attached at least to the opening portions of the through-hole 2. As illustrated in
When the transparent resin sheet 4 is provided, it is desirable to adjust the position of the lens 1 in such a manner that the focus position of the lens 1 is on the outer surface of the transparent resin sheet 4. In
Next, a method for performing microscopic observation with use of the lens unit 10 in the embodiment is described. The lens unit 10 in the embodiment is disposed at such a position that the lens 1 is located on the optical axis of a camera provided in a smart device. For instance, when the holding member 3 is formed of a transparent flat plate, the lens unit 10 is disposed at such a position that the window of a camera in a smart device and the lens 1, as viewed via the holding member 3, overlap each other. Thus, the lens 1 of the lens unit 10, and the lens of the camera in the smart device constitute a transmission compound microscope.
Further, as illustrated in
When a sample is observed by the transmission compound microscope, the sample as an object to be observed is placed on the transparent resin sheet 4 of the lens unit 10, or is placed on a thin transparent sheet (thus, forming a so-called prepared sample). Then, the transparent sheet is placed on the lens unit 10. The transparent sheet carrying the sample is placed on the lens unit 10 at such a position that the sample is disposed above the lens 1. A microscopic observation image of the sample 2 is acquired by an imaging element 22b of the camera 22 while using, for instance, automatic focus or automatic exposure provided in the camera of the smart device 20.
The lens unit in the embodiment is disposed on the front camera of a smart device. Therefore, when microscopic observation is performed, it is possible to place the smart device on a desk in a stationary state. Thus, an image is not blurred by user's movement. Further, the lens unit in the embodiment has a substantially flat plate shape. Therefore, the lens unit has excellent stability, and a mechanical fixing mechanism for a smart device is not necessary. A compound microscope constituted by the lens unit in the embodiment and a smart device is a transmission microscope like a Leeuwenhoek microscope. The transmission compound microscope is configured such that a sample is placed on the lens unit. Therefore, a sample platform is not necessary. In addition to the above, the lens unit in the embodiment has a simplified structure. Therefore, it is easy to clean the lens unit, and it is possible to wash the lens unit with water.
Next, a lens unit as the first modification of the first embodiment of the invention is described.
The transparent resin sheet 4 has a flat surface, and the coefficient of friction is small. As a result, in some cases, it may be difficult to stably fix the lens unit 10 to the smart device 20. In view of the above, as illustrated in
Providing the slip stopper member 5 as described above makes it possible to enhance stability in fixing the lens unit 11. It is easy to fix the lens unit 11 in the modification merely by positioning the window of the front camera 22 of the smart device 20 and the lens 1 to each other, and pressing the lens 1 toward the smart device 20.
Next, a lens unit as the second modification of the first embodiment of the invention is described.
As illustrated in
Next, a lens unit as the third modification of the first embodiment of the invention is described.
As illustrated in
Next, a sample observation kit in the second embodiment of the invention is described.
When a transmission microscope is used, the illumination method affects the performance of the microscope. As the illumination methods for a sample, there are known critical illumination in which a light source image is formed on a sample surface, and Koehler illumination in which illumination has no relationship with the size or the shape of a light source. The sample observation kit in the embodiment employs Koehler illumination. Specifically, as illustrated in
The illumination cap member 30 is constituted by a cap portion 31 made of an opaque material, and a lighting lens 32 placed on the top surface of the cap portion 31. An example of the lighting lens 32 is a ball lens. As illustrated in
As described above, when the illumination cap member 30 is placed on the sample 9, the sample 9 is subjected to Koehler illumination by the operation of the lighting lens 32, no matter where a light source is placed. Further, the illumination cap member 30 is a member independent of the lens unit 10, and it is possible to place the illumination cap member 30 at any position. Therefore, it is possible to search an optimum illumination position by moving the illumination cap member 30 with respect to a light source image in an oblique direction, for instance.
Preferably, a slip stopper member 33 made of rubber may be provided on a bottom surface of the illumination cap member 30 (a surface in contact with the lens unit 10) so that the illumination cap member 30 is not easily moved after being placed. It is preferable to use silicone rubber having high adhesiveness to a resin material forming a holding member 3 or a transparent resin sheet 4, as a material for the slip stopper member 33.
Further, it is preferable to adjust the height of the illumination cap member 30 such that the distance between the lower end of the lighting lens 32 (the sample-side end portion), and a sample is in the range of from 3 to 10 mm. An illumination effect can be obtained even when a hollow pinhole is formed in the top surface of the cap portion 31, in place of installing the lighting lens 32. In this case, however, it is necessary to adjust the position of the illumination cap member 30, taking into consideration the positional relationship between a light source and a sample. In view of the above, the lighting lens 32 is used for the illumination cap member 30 of the sample observation kit in the embodiment.
In
Next, a sample observation kit in the third embodiment of the invention is described.
As illustrated in
In view of the above, the sample observation kit in the embodiment is provided with the transparent cover sheet 40 for covering a sample. The cover sheet 40 has substantially the same function as a so-called sample cover glass. The thickness of the cover sheet 40 may be appropriately set depending on the specifications of the lens unit 11 (such as the focal length of the lens 1 or the thickness of the transparent resin sheet 4). For instance, the thickness of the cover sheet 40 may be about 0.2 mm.
As illustrated in
Further, a cutaway of a size corresponding to the width of the handle portion 40b of the cover sheet 40 may be formed in two positions facing the lower portion of the side surface of the illumination cap member 30 so that the handle portions 40b of the cover sheet 40 are fixed by the cutaways. In this case, preferably, the depth of the cutaway may be such that the sum of the depth of the cutaway and the thickness of the slip stopper member 33 of the illumination cap member 30 is equal to or larger than the thickness of the cover sheet 40.
Next, a transmission compound microscope in the fourth embodiment of the invention is described.
In view of the above, as illustrated in
In
Next, a configuration example of a sample observation kit in the fifth embodiment of the invention is described.
For instance, as illustrated in
Further, as illustrated in
Next, a lens unit in the sixth embodiment of the invention is described. In the lens units in the first embodiment and in the modifications thereof, the lens 1 is accommodated in the holding member 3, and is sealed by the transparent resin sheet 4, as necessary. The invention, however, is not limited to the above. The lens 1 may be exposed from the holding member 3.
When the lens unit of the invention is used in combination with a smart device, the field of view of an obtained image increases, as the distance between the lens 1 of the lens unit and the lens of the smart device decreases. Thus, the magnification for observation is enhanced to some extent. On the other hand, when a slip stopper member is disposed between the lens 1 and a smart device, as exemplified by the lens unit in the third modification of the first embodiment of the invention illustrated in
In view of the above, as illustrated in
According to the aforementioned configuration, as compared with the lens unit 13 illustrated in
Next, a lens unit as a modification of the sixth embodiment of the invention is described.
It is possible to form the lens integrated member as described above by integrally forming with use of resin for an optical lens, for instance. In this method, it is possible to form lens integrated members of various shapes such as an aspherical lens provided with aberration correction. Specifically, a lens portion as illustrated in
It is important to secure the material uniformity after molding in manufacturing a high-performance lens. The lens used in the invention is very small. Therefore, it is easy to secure the material uniformity, as compared with a large lens as used in the other microscopes. Thus, it is not particularly necessary to use high-quality optical resin, and only high-precision molding is required. Since the molding technique has already been established, it is possible to manufacture the lens for use in the invention at a low cost.
In the following, the advantageous effects of the invention are described by an example of the invention and a comparative example. In the examples, euglena was photographed by a transmission compound microscope incorporated with the lens unit of the invention and a smart device, and by a conventional high-quality optical microscope (ME600 by Nikon Corporation).
As illustrated in
Number | Date | Country | Kind |
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2013-169294 | Aug 2013 | JP | national |
2013-232425 | Nov 2013 | JP | national |
Filing Document | Filing Date | Country | Kind |
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PCT/JP2014/071494 | 8/15/2014 | WO | 00 |