Patent Application
20150079183
The present application claims the priority of China Patent Application No. 201210104974.5, filed with the China Patent Office on Apr. 11, 2012, titled “LERCANIDIPINE HYDROCHLORIDE AND LOSARTAN POTASSIUM COMPOUND PREPARATION AND PREPARATION METHOD THEREOF”, the contents of which are incorporated herein by reference in its entirety.
The present invention belongs to the field of medicine, particularly relates to a lercanidipine hydrochloride and losartan potassium compound preparation and preparation method thereof.
Hypertension is definitely the most important risk factor of cardiovascular disease, the long-term development of which could cause serious lesions to target organs, such as heart, brain, kidney and the like, and the complications of which, such as coronary heart disease, apoplexy, kidney function failure and the like, have high disability rate and lethality. In China, hypertension now has been the first main cause of death. Data indicated that the patients suffering from hypertension have reached 200 million until 2006, while around 1 billion people suffered from hypertension all over the world. Therefore, the prophylaxis and treatment of hypertension, and medication are the most important in the medical research and development task.
Lercanidipine hydrochloride is a third-generation dihydropyridine calcium channel antagonist developed by Recordati corporation, Italy, which was firstly marketed in Netherlands in 1997, subsequently in 18 countries separately, such as France, Australia, Germany and others. The action mechanism of lercanidipine hydrochloride is similar to that of the same kinds of drugs, i.e. reversibly blocking Ca2+ influx of L-type calcium channel in vascular smooth muscle cell membrane, and dilating peripheral vessels to reduce blood pressure. This product has high lipophilicity, thus its onset time is slower while the duration of action is longer. The in vivo/in vitro trials indicate that the negative inotropic action caused by selective exangia of lercanidipine hydrochloride is weaker than that of nifedipine, nitrendipine and felodipine; while its vascular selectivity is higher than those of amlodipine, felodipine, nitrendipine and lacidipine. In addition, lercanidipine hydrochloride also has anti-atherosclerotic and end-organ protective effect. Lercanidipine hydrochloride at therapeutic dose does not interfere with normal cardiac excitability and conductivity of the patients suffering from hypertension. Animal experiments indicate that lercanidipine hydrochloride protect kidney, whose mechanism may be not related to hemodynamics. Lercanidipine hydrochloride has higher vascular selectivity compared with the drugs of the same kind, and its particular lipophilicity makes its blood pressure decreasing effect slow and persistent. Lercanidipine hydrochloride has high safety and no cardiotonic effect, and it does not affect heart rate and has excellent anti-atherosclerotic effect meanwhile, in particular being suitable for hypertension patients accompanying with atherosclerosis, which has high clinical application value and broad market prospect.
Losartan is an oral non-peptide angiotensin II receptor antagonist (AIIA), which was firstly marketed in 1994 and has been approved in 93 countries currently for treating hypertension. Renin-angiotensin system (RAS) in body is activated by renin released by kidney. Renin is capable of decomposing angiotensinogen, a protein derived from liver, and the product after decomposition is angiotensin I. Angiotensin I itself has no bioactivity, however, it is the precursor of Angiotensin II, which has a series of bioactivities on vessels, heart and other tissues in body. AIIA drug losartan is capable of blocking angiotensin receptor (AT1) to avoid angiotensin II binding to the receptor, finally achieving the purpose of preventing vasoconstriction. The clinical application shows that it has better tolerance and fewer side effects.
Currently, commercial available preparations are all single-ingredient preparations. There is still no report about combination of two ingredients for treating hypertension.
A purpose of the present invention is to provide a lercanidipine and losartan compound preparation, which contains 0.5-40% lercanidipine hydrochloride and 6.25-50% losartan potassium based on mass percentage.
In the compound preparation provided by the invention, calcium ion antagonist lercanidipine hydrochloride causes reflex sympathetic excitation by dilating vessels, and angiotensin II receptor antagonist losartan could relieve the over-activation of sympathetic system during calcium channel blocker (CCB) decreasing blood pressure by antagonizing neurohormone activity, so as to decrease the adverse effects caused by CCB treatment, which provides a more comprehensive protection to the target organs, such as heart, brain, kidney, etc, thus has a better anti-atherosclerotic effect and therapy of hypertension.
More preferably, the mass ratio of lercanidipine hydrochloride to losartan potassium in the compound preparation provided by the present invention is 1:2-25.
A lercanidipine and losartan compound preparation is specifically disclosed in the embodiments of the invention, the excipients used in which are 20-40% lactose monohydrate, 10-40% microcrystalline cellulose, 10-20% A-type sodium starch glycolate, 2-8% povidone K30, 0.5-2.5% magnesium stearate, 5-15% pregelatinized starch and 1-10% colloidal silicon dioxide.
Preferably, a coating used is opadry white.
The present invention also provides a method for preparing said compound preparation, including the following steps:
Step 1: sieved active pharmaceutical ingredients lercanidipine hydrochloride and losartan are stirred and homogenized with lactose monohydrate, microcrystalline cellulose, A-type sodium starch glycolate, povidone K30, magnesium stearate, pregelatinized starch and colloidal silicon dioxide, followed by vacuum compression and milling, sieved and granulated, tableted with controlling the hardness of tablet core;
Step 2: opadry is added to ethanol with stirring until dispersion, then purified water is added and stirred to obtain isolation coating solution, which is then used for film coating of the tablet core obtained in Step 1;
Step 3: opadry is added to ethanol, stirred and homogenized to obtain enteric coating solution, which is then used to coat enteric coating for the film-coated tablets.
Preferably, the controlled hardness of tablet core in step 1 is 2-10 kg.
Preferably, the concentration of ethanol in step 2 and step 3 is 75-100%.
Preferably, the duration for stirring after adding purified water in step 2 and for the stirring in step 3 is 20-100 min.
Preferably, the opadry in step 2 is Y-1-7000 and the opadry in step 3 is type OY-P, 91S.
The present invention provides a compound preparation, which comprises lercanidipine hydrochloride and losartan potassium as active pharmaceutical ingredients, lactose monohydrate, microcrystalline cellulose, sodium starch glycolate (A-type), povidone K30, magnesium stearate, pregelatinized starch and colloidal silicon dioxide as excipients and opadry white as a coating to formulate tablet.
In the lercanidipine and losartan compound preparation provided by the present invention, the calcium ion antagonist lercanidipine causes reflex sympathetic excitation by dilating vessels, and after combining with angiotensin II receptor antagonist lorsartan, the anti-neurohormone activity of lorsartan could relieve the over-activation of sympathetic system during calcium channel blocker (CCB) decreasing blood pressure, so as to reduce adverse reactions caused by CCB treatment, which provides a more comprehensive protection to the target organs including heart, brain, kidney, etc.
The clinical trials show that compared with single-ingredient preparation group, the lercanidipine and losartan compound preparation group markedly increases the effectiveness of treatment of mild and moderate hypertension, with markedly reduced incidence of adverse reactions, is tolerable well by patients, and increases the therapy of reducing blood pressure and has excellent clinical application prospects.
The present invention discloses a lercanidipine hydrochloride and losartan potassium compound preparation and preparation method thereof, which could be achieved by those skilled in the art by referring to the contents the disclosure with proper improvement of the process parameters. It should be noted particularly, all of such similar replacements and moderations are apparent to those skilled in the art, which are all considered as being included in the present invention. The products, methods and applications of the present invention have been described through preferred examples. Those skilled in the art can change, modificate or combine properly the product, method and application of the present invention without departure from the contents, spirits and scope of the present invention, so as to achieve and carry out the present invention.
In order that the technical solutions of the present invention can be better understood by those skilled in the art, the present invention will be further illustrated in detail by combining with specific examples.
Active pharmaceutical ingredients lercanidipine hydrochloride and losartan were weighed and sieved; lercanidipine hydrochloride was 0.5-40% and losartan potassium was 6.25-50%.
Lactose monohydrate, microcrystalline cellulose, sodium starch glycolate (A-type), povidone K30, magnesium stearate, pregelatinized starch and colloidal silicon dioxide were weighed, stirred and homogenized. The excipients comprise 20-40% lactose monohydrate, 10-40% microcrystalline cellulose, 10-20% sodium starch glycolate (A-type), 2-8% povidone K30, 0.5-2.5% magnesium stearate, 5-15% pregelatinized starch and 1-10% colloidal silicon dioxide.
Compressed in vacuum and milled, sieved and granulated; tableted with controlling the hardness of tablet core.
Formulating isolation coating solution: opadry was added to ethanol with stirring until dispersion, then purified water was added and stirred to formulate isolation coating solution.
Film coating: the prepared isolation coating solution was used to coat film coating for the obtained tablet core to obtain the film-coated tablets.
Formulating enteric coating solution: opadry in prescribed dose was added to ethanol with stirring, and stirring was continued to obtain enteric coating solution.
Enteric coating: the prepared enteric coating solution was used to coat enteric coating for the film-coated tablets, and the unit dose of the obtained compound preparation contained 5 mg lercanidipine and 20 mg losartan.
Active pharmaceutical ingredients lercanidipine hydrochloride and losartan were weighed and sieved; lercanidipine hydrochloride was 0.5-40% and losartan potassium was 6.25-50%.
Lactose monohydrate, microcrystalline cellulose, sodium starch glycolate (A-type), povidone K30, magnesium stearate, pregelatinized starch and colloidal silicon dioxide were weighed, stirred and homogenized. The excipients comprise 20-40% lactose monohydrate, 10-40% microcrystalline cellulose, 10-20% sodium starch glycolate (A-type), 2-8% povidone K30, 0.5-2.5% magnesium stearate, 5-15% pregelatinized starch and 1-10% colloidal silicon dioxide.
Compressed in vacuum and milled, sieved and granulated; tableted with controlling the hardness of tablet core.
Formulating isolation coating solution: opadry was added to ethanol with stirring until dispersion, then purified water was added and stirred to formulate isolation coating solution.
Film coating: the prepared isolation coating solution was used to coat film coating for the obtained tablet core to obtain the film-coated tablets.
Formulating coterie coating solution: opadry in prescribed dose was added to ethanol with stirring, and stirring was continued to obtain enteric coating solution.
Enteric coating: the prepared enteric coating solution was used to coat enteric coating for the film-coated tablets, and the unit dose of the obtained compound preparation contained 7.5 mg lercanidipine and 20 mg losartan.
HPLC method was used to determine the content of lercanidipine hydrochloride in the lercanidipine and losartan compound preparation prepared in Example 1. The detection conditions for liquid chromatography are as shown in table 1.
Preparation method of A buffer: 1.36 g monopotassium phosphate was dissolved in 1 L water, after dissolving, 1 g octane sulphonic acid sodium salt was added thereto, sonicated and 10% phosphoric acid (v/v) was used to adjust pH to 2.5±0.05.
Material and Method
1.1 Material
60 female clean grade spontaneous hypertension rat (SHR) model rats were used, which were around 200 g and the awake tail artery systolic blood pressure (SBP) was 160 mmHg (1 mmHg-0.133 kPa).
1.2 Experimental Method
1.2.1 Preparation of Experimental Model and Grouping
The rats were anaesthetized with 50 mg/kg Ketamine Hydrochloride and 5 mg/kg diazepam via intraperitoneal injection, an about 2 cm long skin incision at left groin was cut, arteriae femoralis sheath was exposed, the arteriae femoralis and femoral veins were separated carefully and arteriae femoralis cannula was performed. The artery canalis were rounded from the thigh root of animals to back via subcutaneous tunnel, and were drawn out from head and neck to be fixed. The rats were free to access food for 2 days postoperatively.
The SHR rats were randomly divided into lercanidipine and losartan compound preparation 1 (mass percentage of main ingredient: lercanidipine hydrochloride 50% and losartan potassium 12%), lercanidipine and losartan compound preparation 2 (mass percentage of main ingredient: lercanidipine hydrochloride 30% and losartan potassium 46%), lercanidipine and losartan compound preparation 3 (mass percentage of main ingredient: lercanidipine hydrochloride 0.25% and losartan potassium 58%), 20 rats for each group. Systems were connected to perform awake blood pressure monitoring. Firstly operated for adaptation for 4 h. During the monitoring, dosed orally and the dosing time was at 10:00 am. The blood pressure record was started from 1 h before dosing and finished at hour 8 after dosing.
1.2.2 Detection Index
For awake pressure measurement, systolic blood pressure (SBP), diastolic blood pressure (DBP), heartbeat interphase (HP), pulse pressure (PP) and systolic blood pressure variation (SBPV) per pulsation were real time recorded by computer. After 4 h adaptive stable, blood pressure signals per pulsation were started to record. The record time was 9 h (when observing the decreasing blood pressure effects of single dosing yajing capsule acutely, record was from 1 h before dosing to hour 8 after dosing, i.e. from 9:00 of the dosing day to 22:00 that day), then the total hemodynamic index was analyzed off-line.
1.2.3 Statistical Process
SPSS 10.0 statistical software was used to process. The detected data was expressed as mean # standard deviation. Paired t-test was used to compare before and after dosing for each group. One-way ANOVA was used to compare the difference of mean value between each group. And LSD-q test was used to multiple compare the difference of mean value, between each group.
1.3 Results
The blood pressure variation condition of SHR rats after 3 groups dosing. After orally dosing, the blood pressures for lercanidipine and losartan compound preparation 1 and lercanidipine and losartan compound preparation 3 both changed to some degree comparing with pre-dosing. For lercanidipine and losartan compound preparation 2, the blood pressure changed significantly before and after dosing, and has the statistical significance.
1.1 Selection of eases: patients aged from 18-75 years old with light and moderate diastolic hypertension (supine diastolic blood pressure 95-115 mmHg), excluding severe hypertension (diastolic blood pressure >115 mmHg), secondary hypertension, heart disease, myocardial infarction, shock, transient ischemic stroke within the past 3 months, sinus bradycardia or sinus tachycardia, hypovolemia, liver and kidney dysfunction and diabetes. After 14-20 days of washing stage, entering 14-30 days of introduction stage, during which single blinded placebos were taken. Blood pressures were measured in a return visit in every 7-10 days. Suitable patients were divided into treatment groups according to random principle.
1.2 Trial method: there were totally 396 patients enrolled, and 388 patients entered the introduction stage and 307 entered the assessment stage. The patients were divided into 4 groups randomly: 1) low dose compound preparation group: a lercanidipine and losartan compound preparation containing 5 mg lercanidipine and 20 mg losartan; 2) high dose single administration group 1: lercanidipine hydrochloride (Recordati Industria Chimica e Farmaceutica S.P.A.), 10 mg; 3) high dose single administration group 2: 100 mg losartan (produced by Hangzhou MSD Co., Ltd.): and 4) positive control group 10 mg amlodipine (Pfizer Inc.), once daily, continuous administration.
1.3 Observation indicators and method: blood, pressures were measured as per the requirements of international standard. A settled physician would follow up once a week and visited from 9:00 to 11:00. Every time heart rate and sitting blood pressure were measured 3 times and the 2 greater values were taken to calculate the mean value.
1.4 Laboratory examinations: electrocardiogram, blood and urine routine, liver and kidney function, blood sugar, blood lipid and blood electrolyte examinations were performed before and after overall patient trial.
1.5 Curative effect assessment: according, to the regulations of The National Cardiovascular Epidemiology and Population Prevention report meeting summary in 1979: (1) markedly effective: diastolic blood pressure was decreased by equal to or greater than 10 mmHg, and was decreased to normal (<90 mmHg) or decreased by more than 20 mmHg; (2) effective: diastolic blood pressure was decreased by 10-19 mmHg, or was decreased by less than 10 mmHg, but reached normal; (3) failure: not reached above criteria.
1.6 Data processing and statistical method: the measurement data were calculated as mean±standard variation. Paired t-test was used to compare the quantitative indicators before and after the 4 treatment patterns. Chi-square test was used to compare the difference before and after administration.
Analysis of curative effect: (1) change of blood pressure at the end of week 12 of treatment: at the end of week 12 of treatment, blood pressures in all of the 4 groups of patients decreased remarkably, and the differences had statistical significance (p<0.05) (see table 2).
Table 2 shows the changes of average diastolic blood pressure and systolic blood pressure before treatment and at the end of week 12 of treatment in 4 groups of patients, wherein 1) is a lercanidipine and losartan compound preparation containing 5 g lercanidipine and 20 g losartan prepared in Example 1; 2) is 10 mg lercanidipine hydrochloride; 3) is 100 mg lorsartan: and 4) is 10 mg amlodipine.
Table 2 changes of average diastolic blood pressure and systolic blood pressure before treatment and at the end of week 12 of treatment in 4 groups of patients
The decline of diastolic blood pressure of the lercanidipine and losartan compound preparation group was larger than that of amlodipine group, and the difference had statistical significance (P<0.05); the systolic blood pressures of the 4 groups also decreased significantly, wherein the decline of systolic blood pressure of the lercanidipine and losartan compound preparation group was remarkably greater than that of amlodipine group, and the difference had statistical significance (P<0.05). The decline of diastolic and systolic blood pressures of the lercanidipine and losartan compound preparation group were significantly greater than those of lercanidipine hydrochloride high dose single administration group and lorsartan high dose single administration group, and the differences had statistical significance (P<0.05).
(2) Comparison of blood pressure decreasing effective rate: the total effective rate for decreasing blood pressure of the lercanidipine and losartan compound preparation group at the end of week 12 of treatment was 85.2%, which was significantly higher than that of the lercanidipine hydrochloride single administration group (70.8%) and the lorsartan single administration group (75.1%) (P<0.05).
There were two cases occurring gastrointestinal tract symptoms and one dizziness in the lercanidipine hydrochloride single administration group. There were four cases occurring gastrointestinal tract symptoms and one dry mouth in the losartan single administration group. All of those were slight and had no effect on continuous dosing. The difference of the incidences of adverse reactions between the two groups were not significant. There was no remarkable adverse reaction for the combined dosing groups, and for the 3 groups of patients treated for 12 weeks, there was no significant difference in laboratory examination results and changes in electrocardiogram.
The lercanidipine and losartan compound preparation group has significantly effective rate, and the incidence of adverse reactions is significantly decreased, tolerated well by the patients, compared with the high dosage single administration group. Therefore, treatment of mild and moderate hypertension by the lercanidipine and losartan compound preparation could improve curative effect of blood pressure decrease, and it is tolerable and has no remarkable influence on cardiac conductivity and heart rate.
1.1 Selection of cases: patients aged from 18-75 years old with light and moderate diastolic hypertension (supine diastolic blood pressure 95-115 mmHg), excluding severe hypertension (diastolic blood pressure >115 mmHg), secondary hypertension, heart disease, myocardial infarction, shock, transient ischemic stroke within the past 3 months, sinus bradycardia or sinus tachycardia, hypovolemia, liver and kidney dysfunction and diabetes. After 14-20 days of washing stage, entering 14-30 days of introduction stage, during which single blinded placebos were taken. Blood pressures were measured in a return visit in every 7-10 days. Suitable patients were divided into treatment groups according to random principle.
1.2 Trial method: there were totally 359 patients enrolled, and 305 patients entered the introduction stage and 266 entered the assessment stage. The patients were divided into 4 groups randomly: 1) low dose compound preparation group: a lercanidipine and losartan compound preparation containing 7.5 mg lercanidipine and 20 mg losartan; 2) high dose single administration group 1: lercanidipine hydrochloride (Recordati Industria Chimica e Farmaceutica S.P.A.), 10 mg; 3) high dose single administration group 2: 100 mg losartan (produced by Hangzhou MSD Co., Ltd.).
1.3 Observation indicators and method: blood pressures were measured as per the requirements of international standard. A settled physician would follow up once a week and visited from 9:00 to 11:00. Every time heart rate and sitting blood pressure were measured 3 times and the 2 greater values were taken to calculate the mean value.
1.4 Laboratory examinations: electrocardiogram, blood and urine routine, liver and kidney function, blood sugar, blood lipid and blood electrolyte examinations were performed before and after overall patient trial.
1.5 Curative effect assessment: according to the regulations of The National Cardiovascular Epidemiology and Population Prevention report meeting summary in 1979: (1) markedly effective: diastolic blood pressure was decreased by ≧10 mmHg, and was decreased to normal (<90 mmHg) or decreased by more than 20 mmHg; (2) effective: diastolic blood pressure was decreased by 10-19 mmHg or was decreased by <10 mmHg, but reached normal; (3) failure: not reached above criteria.
1.6 Data processing and statistical method: the measurement data were calculated as mean±standard variation. Paired t-test was used to compare the quantitative indicators before and after the 4 treatment patterns. Chi-square test was used to compare the difference before and after administration.
Analysis of curative effect: (1) change of blood pressure at the end of week 12 of treatment: at the end of week 12 of treatment, blood pressures in all of the 4 groups of patients decreased remarkably, and the differences had statistical significance (P<0.05) (see table 3).
Table 3 changes of average diastolic blood pressure and systolic blood pressure before treatment and at the end of week 12 of treatment in 3 groups of patients
Wherein, 1) is a lercanidipine and losartan compound preparation containing 7.5 g lercanidipine and 20 g losartan; 2) is 10 mg lercanidipine hydrochloride; and 3) is 100 mg lorsartan.
The decline of diastolic blood pressure of the lercanidipine and losartan compound preparation group was larger than that of amlodipine group, and the difference had statistical significance (P<0.05); the systolic blood pressures of the 3 groups also decreased significantly, wherein the decline of systolic blood pressure of the lercanidipine and losartan compound preparation group was remarkably greater than that of amlodipine group, and the difference had statistical significance (P<0.05). The decline of diastolic and systolic blood pressures of the lercanidipine and losartan compound preparation group were significantly greater than those of lercanidipine hydrochloride high dose single administration group and lorsartan high dose single administration group, and the differences had statistical significance (P<0.05).
(2) Comparison of blood pressure decreasing effective rate: the total effective rate for decreasing blood pressure of the lercanidipine and losartan compound preparation group at the end of week 12 of treatment was 87.4%, which was significantly higher than that of the lercanidipine hydrochloride single administration group (71.6%) and the lorsartan single administration group (77.3%) (P<0.05).
There was one case occurring gastrointestinal tract symptoms and one dizziness in the lercanidipine hydrochloride single administration group. There were two cases occurring gastrointestinal tract symptoms and one dry mouth in the losartan single administration group. All of those were slight and had no effect on continuous dosing. The difference of the incidences of adverse reactions between the two groups were not significant. There was no remarkable adverse reaction for the combined dosing groups, and for the 3 groups of patients treated for 12 weeks, there was no significant difference in laboratory examination results and changes in electrocardiogram.
The lercanidipine and losartan compound preparation group has significantly effective rate, and the incidence of adverse reactions is significantly decreased, tolerated well by the patients, compared with the high dosage single administration group. Therefore, treatment of mild and moderate hypertension by the lercanidipine and losartan compound preparation could improve curative effect of blood pressure decrease, and it is tolerable and has no remarkable influence on cardiac conductivity and heart rate.
The above-mentioned contents are only preferred embodiments of the present invention. It should be noted that those ordinary skilled in the art could also make several improvements and modifications without departing from the principle of the invention, which should be considered to fall within the scope of the present invention claimed.
| Number | Date | Country | Kind |
|---|---|---|---|
| 201210104974.5 | Apr 2012 | CN | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/CN2013/071838 | 2/25/2013 | WO | 00 |
