Claims
- 1. An isolated and purified strain of the species Lactobacillus sanfranciscensis, which produces levan.
- 2. The strain according to claim 1, having the deposit number CNCM I-2588 or CNCM I-2589.
- 3. A soluble levan obtained by the strain according to claim 1.
- 4. A method of producing levan, which comprises:
inoculating a culture medium which includes sucrose with a preculture of a strain according to claim 1;allowing the culture medium to ferment at 25-35° C. for 12 to 48 h; lowering the pH of the resulting culture to below 4.5, and; adding cold ethanol to the culture medium and storing it at 0 to 10° C. for 6 to 48 h.
- 5. The method according to claim 4 wherein the culture medium contains at least 0.2% sucrose.
- 6. The method according to claim 4, which further comprises purifying the levan in the sediment after storing the culture medium.
- 7. The method according to claim 4, which further comprises drying the culture medium to produce a levan powder after storing the culture medium.
- 8. A method of preparing a human or pet food product or a cosmetic composition which comprises utilizing the levan according to claim 3.
- 9. A method of preparing a human or pet food product or a cosmetic composition which comprises utilizing the Lactobacillus sanfranciscensis strain according to claim 1.
- 10. The method according to claim 8, wherein the human or pet food product or the cosmetic composition includes a levan in an amount of about 0.01 to 25%.
- 11. The method according to claim 8, wherein the levan is used as an in situ source of prebiotic fructans.
- 12. The method according to claim 8, wherein the levan is an immunologically-stimulating polysaccharide.
- 13. The method according to claim 9, in which the amount of the Lactobacillus sanfranciscensis strain is at least 1×105 cfu/g.
- 14. A human or pet food product comprising the levan according to claim 3.
- 15. A dry infant formula comprising: 5 to 20% of peptides, 2 to 40% of fat, 10 to 60% of non-levan carbohydrates, 1 to 10% of levan according to claim 3, and, optionally, traces of vitamins and oligoelements to meet daily requirements, and 0.1 to 5% of minerals.
- 16. An infant formula comprising 0.5 to 5% of peptides, 0.2 to 10% of fat, 1 to 25% of non-levan carbohydrates 0.5 to 20% of levan according to claim 3, 50% to 90% water, and, optionally, traces of vitamins and oligoelements to meet daily requirements, 0.01 to 2% of minerals.
- 17. A fermented food product comprising the levan according to claim 3.
- 18. A human or pet food product comprising the Lactobacillus sanfranciscensis strain according to claim 1.
- 19. The human or pet food product according to claim 18, which comprises the levan in the amount of about 0.01 to 25%.
- 20. The human or pet food product according to claim 18, in which the amount of the Lactobacillus sanfranciscensis strain is at least 1×105 cfu/g.
Priority Claims (1)
Number |
Date |
Country |
Kind |
EP NO00127939.7 |
Dec 2000 |
EP |
|
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation of International Application PCT/EP01/14732 filed Dec. 11, 2001, the entire content of which is expressly incorporated herein by reference thereto.
Continuations (1)
|
Number |
Date |
Country |
Parent |
PCT/EP01/14732 |
Dec 2001 |
US |
Child |
10465599 |
Jun 2003 |
US |