Claims
- 1. A synthetic nucleotide sequence encoding the LexA DNA binding domain, comprising at least one codon optimized for usage by an Arabidopsis species.
- 2. A synthetic nucleotide sequence according to claim 1, wherein over about 50% of the codons of the sequence are optimized for usage by an Arabidopsis species.
- 3. A synthetic nucleotide sequence according to claim 1, wherein over about 80% of the codons of the sequence are optimized for usage by an Arabidopsis species.
- 4. A synthetic nucleotide sequence according to claim 1, wherein the nucleotide sequence has the sequence SEQ ID NO: 1.
- 5. A synthetic nucleotide sequence according to claim 1, wherein the synthetic nucleotide sequence is optimized for usage by Arabidopsis thaliana.
- 6. A synthetic LexA DNA binding domain protein codon optimized for usage by an Arabidopsis species.
- 7. A synthetic LexA DNA binding domain protein according to claim 6, wherein the synthetic LexA DNA binding domain protein is codon optimized for usage by Arabidopsis thaliana.
- 8. A synthetic LexA DNA binding domain protein according to claim 6 that has the amino acid sequence SEQ ID NO: 2.
- 9. A DNA construct comprising the synthetic nucleic acid sequence of claim 1.
- 10. A DNA construct according to claim 9, wherein the synthetic nucleic acid sequence has the sequence of SEQ ID NO: 1.
- 11. A DNA construct according to claim 9, further comprising a heterologous nucleic acid sequence.
- 12. An eukaryotic cell comprising the DNA construct of claim 10.
- 13. An eukaryotic cell comprising the DNA construct of claim 11.
- 14. An eukaryotic cell according to claim 12, wherein the eukaryotic cell is a plant cell.
- 15. An eukaryotic cell according to claim 14, wherein the plant cell is a dicot cell.
- 16. An eukaryotic cell according to claim 15, wherein the dicot cell is an Arabidopsis thaliana cell.
- 17. A transgenic plant comprising a cell of claim 13.
- 18. A transgenic plant according to claim 17, wherein the plant is a dicot.
- 19. A transgenic plant according to claim 18, wherein the plant is an Arabidopsis thaliana plant.
- 20. A transgenic seed produced by the plant of claim 17.
CROSS-REFERENCES TO RELATED APPLICATIONS
[0001] This application is a divisional of U.S. patent application Ser. No. 09/401,171, filed on Sept. 22, 1999, the contents of which is incorporated by reference.
Divisions (1)
|
Number |
Date |
Country |
Parent |
09401171 |
Sep 1999 |
US |
Child |
10160508 |
Jun 2002 |
US |