Claims
- 1. A method of detecting a target analyte comprising:
(a) providing a conductive support comprising a target analyte attached thereto, the target analyte further comprising a nanoparticle comprising a photoelectrochemically active moiety; (b) exposing the photoelectrochemically active moiety to light, thereby generating a photoelectric current between the photoelectrochemically active moiety and the conductive support; and (c) measuring the photoelectric current as an indication of the presence or the amount of target analyte on the conductive support.
- 2. The method of 1, wherein the nanoparticle comprises a material selected from the group consisting of a metal, a metal oxide, a ceramic, a semiconductor, a dendrimer and an organic polymer.
- 3. The method of claim 2, wherein the nanoparticle comprises a material selected from the group consisting of titanium, titanium dioxide, tin, tin oxide, silicon, silicon dioxide, iron, ironIII oxide, silver, gold, copper, nickel, aluminum, steel, indium, indium tin oxide, fluoride-doped tin, ruthenium oxide, germanium cadmium selenide, cadmium sulfide and titanium alloy.
- 4. The method of claim 1, wherein the support comprises a material selected from the group consisting of titanium dioxide, tin oxide, silicon, ironIII oxide, silver, nickel, gold, indium tin oxide, conductive polymers, metals, semiconductors and plastics coated with a conductant.
- 5. The method of claim 1, wherein the support comprises an array of one or more target analytes located at discrete positions on the support
- 6. The method of claim 5, wherein the one or more target analytes are different from each other.
- 7. The method of claim 1, wherein the support further comprises a probe, and the target analyte is attached to the support by an interaction with the probe.
- 8. The method of claim 1, wherein the photoelectric current is an indication of the presence or the amount of the one or more different target analytes.
- 9. The method of claim 8, wherein the photoelectrochemically active moiety of only one of the different target analytes is exposed to light.
- 10. The method of claim 1, wherein the target analyte is selected from the group consisting of a single stranded DNA oligomer, a single stranded RNA oligomer, a peptide nucleic acid analog, double stranded DNA, a small molecule, an antibody, a polypeptide, a peptide, a synthesized target analyte, a target analyte isolated from a cell, a target analyte that is reverse transcribed from a nucleic acid sequence, a target analyte comprising intact genomic DNA, a target analyte comprising fragmented genomic DNA, mRNA, a PCR product and an OLA product.
- 11. The method of 1, wherein the target analyte is an oligonucleotide whose sequence is determined by surface binding or hybridization
- 12. The method of claim 11, wherein the target analyte further comprises a tag sequence.
- 13. The method of claim 1, wherein the photoelectrochemically active moiety comprises a ruthenium center.
- 14. The method of claim 1, wherein the light is provided by a light source is selected from the group consisting of a tungsten halogen light source, a xenon arc lamp and a laser.
- 15. The method of claim 1, where in the exposing is by rastering.
- 16. The method of claim 1, wherein the exposing is performed in the presence of a redox mediator.
- 17. The method of claim 1, wherein the probe is selected from the group consisting of a single stranded DNA oligomer, a single stranded RNA oligomer, a peptide nucleic acid analog, double stranded DNA, a small molecule, an antibody, a polypeptide, a peptide, a synthesized target analyte, a target analyte isolated from a cell, a target analyte that is reverse transcribed from a nucleic acid sequence, a target analyte comprising intact genomic DNA, a target analyte comprising fragmented genomic DNA, mRNA, a PCR product and an OLA product.
- 18. The method of claim 17, wherein the probe further comprises a tag sequence.
- 19. The method of claim 1, further comprising passivating the support with a passivation moiety before contacting the target analyte with the probe under hybridization conditions.
- 20. The method of claim 19, wherein the passivation moiety comprises a moiety selected from the group consisting of silyl chloride, a sol gel, polyethylene glycol, a thiol, a siloxane, an organic polymer, a carboxylate and combinations thereof.
- 21. The method of claim 1, further comprising contacting the support with a secondary component after contacting the target analyte with the probe under hybridization conditions.
- 22. The method of claim 21, wherein the secondary component is a photoelectrochemically active moiety.
- 23. The method of claim 1, wherein the providing comprises
(a) providing a nanoparticle comprising a target analyte, a central component and a photoelectrochemically active moiety; (b) providing a probe associated with a support; and (c) contacting the probe associated with the support with the nanoparticle under hybridization conditions.
- 24. The method of claim 23, wherein the target analyte is selected from the group consisting of an mRNA sequence derived from a sample to be monitored for gene expression and a cDNA sequence derived from a sample to be monitored for gene expression.
- 25. The method of claim 24, wherein the probe comprises a gene of interest.
- 26. The method of claim 24, wherein the presence of a photoelectric current is indicative of duplex formation and duplex formation is indicative of gene expression.
- 27. The method of claim 1, wherein the providing comprises
(a) providing a nanoparticle comprising a photoelectrochemically active moiety and a target analyte; (b) providing a probe associated with a support, wherein the probe comprises or is suspected to comprise a mutation to be detected; and (c) contacting the probe sequence with the target analyte under hybridization conditions.
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] The present patent application is based on and claims priority to U.S. Provisional Application Serial No. 60/307,019, entitled “LIGHT ADDRESSABLE ELECTROCHEMICAL DETECTION OF DUPLEX STRUCTURES”, which was filed Jul. 20, 2001 and is incorporated herein by reference.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60307019 |
Jul 2001 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
PCT/US02/23174 |
Jul 2002 |
US |
Child |
10236205 |
Sep 2002 |
US |