The present invention is directed, in general, to the field of optical systems and methods for measuring properties of a sample, allowing inspecting the sample in a non-destructive manner. In particular, the invention relates to a light inspecting system and method of the surface and inside of a sample, for example, food, recycling or wood products, a mineral, etc.
Patent EP 2200758 concerns a method and a sorting apparatus for detecting irregularities in a product, in which at least one light beam is directed towards this product by means of a moving mirror, whereby the product moves in a particular direction through a detection zone, so that said light beam, which preferably moves transversely across the path of the product, is at least partially scattered and/or reflected by said product. The scattered and/or the reflected light is detected by at least one detector in order to characterize and to sort the product.
U.S. Pat. No. 7,830,519 discloses a device for non-invasively measuring concentration of one or more analytes in a living subject or a biological sample, wherein the device includes several light sources, a system for controlling the timing and intensity of the light source outputs, a system for passing the light through the subject or sample, a system for measuring the amount of light transmitted, and a system for relating the measurement to the concentration of the analyte in question. The light sources are narrow band sources at different wavelengths, and are capable of being rapidly switched between two levels of intensity. The number of light sources required and the wavelengths of the sources are dependent upon the specific analyte being measured.
U.S. Pat. No. 6,753,966 relates to spectral analysis systems and methods for determining physical and chemical properties of a sample by measuring the optical characteristics of light emitted from the sample. In one embodiment, a probe head for use with a spectrometer includes a reflector for illuminating a sample volume disposed circumferentially about the light source of the probe head. In another embodiment, a probe head includes an optical blocking element for forcing the optical path between the light source and an optical pick-up optically connected to the spectrometer into the sample. The probe head also includes a reference shutter for selectively blocking light emitted from the sample from reaching the optical pick-up to facilitate calibration of the spectrometer.
Patent EP 0822394 relates to a photodetection device which can eliminate a drift or a random noise component from a feeble measuring signal and amplify the signal at a high signal-to-noise ratio suitable to be used in a measuring device for optically measuring a specific substance such as glucose or hemoglobin in blood or urine or sugar in a fruit, for example, contained in a scattering substance such as a liquid, food or a human body.
Patent application US 2007/0229832 relates to a method and an apparatus for, in order to inspect and sort external qualities and internal qualities of agricultural products or the like, inspecting, in a non-destructive manner, component values such as a sugar degree and an acid degree and internal qualities such as internal diseases and defects and physiological defects that cannot be found in appearance of objects such as the agricultural products by projecting beams to objects, which are conveyed one by one in a line by various conveying means, from both left and right sides of a conveying path in inspection positions of predetermined positions of the conveying path using plural floodlight lamps, condensing and receiving transmitted light exiting to an upper side and a lower side through the insides of the objects, and applying spectral analysis to the transmitted light.
Patent application EP 1959250 discloses an analyzing apparatus having a structure for enabling spectrometry to a longer frequency region while being excellent in practicality. The analyzing apparatus comprises a light source section and a light-detecting section. The light source section includes a seed light source emitting laser light, and a solid highly nonlinear optical fiber generating supercontinuum light in response to the input of the laser light, and thereby emitting the supercontinuum light as irradiation light to an object. The light-detecting section detects light to be detected from the object irradiated with the irradiation light.
Patent application US 2015/062573 provides an optical detection device including an optics arrangement configured to generate an annular illumination pattern to illuminate a portion of a sample and further configured to receive a return light from the portion of the sample illuminated by the annular illumination pattern. Moreover, a detector arrangement configured to detect the return light is also included.
Patent application US 2003/030850 discloses a system for optical interrogation of a sample adaptable for multiple wavelength illumination and multiple wavelength fluorescent or luminescent light collection, wherein the illumination wavelength profile and the light collection profile may overlap. In the system, coherent light from one or more lasers is focused onto a target layer on a sample to excite fluorescent or luminescent light from the target layer. Emitted light is collected from a selected depth by a reflective light collector that transmits the collected light to detection optics. The reflective light collector directs collected light at an angle to the optical axis of the illumination light, thereby separating collected emitted light from illumination light. The light collector may collect light from a focus, whereby the focused illumination light combined with the focused light collection aid in limitation of the depth of field to a selected depth. Additionally, a spatial filter positioned between the light collector and the detection optics may be used to confine the depth of field to a selected depth. This device may be incorporated into an optical scanner by scanning of illumination light in a first direction and translation of the sample in a tangent direction. Alternatively, the illumination and detection optics may remain stationary and the detectable targets moved past a scanning location (e.g. as in electrophoretic analysis).
Patent application US 2008/174767 discloses an optical system for achieving enhanced rejection of scattered excitation light and superior signal-to-noise performance when reading microplate wells. The optical system uses an axial configuration in which the excitation beam incident upon the sample propagates along the axis of the microplate well. Excitation light from a light source, such as a lamp or fiber optic bundle, is collimated into a beam using a lens. A reflective pick-off mirror is then used to reflect the collimated excitation beam upward along the well axis. A focusing lens, with a diameter exceeding the diameter of the collimated excitation beam, is used to focus the excitation beam in the well. The same broad lens is used to collimate the emitted fluorescent light, of which a large percentage propagates axially past the pick-off mirror towards a second focusing lens that focuses the emission beam onto the face of a fiber optic bundle. The emitted light is later filtered and detected using at a position that is optically shielded from the aforementioned optical system. The optical system is incorporated into a microplate reader or automated assay instrument in order to provide a compact assembly for sensitive fluorescence measurements either above or below the microplate. The optical system further enables the simultaneous measurement of absorbance and fluorescence in a compact optical configuration.
Other optical systems for measuring optical parameters of a sample are known by WO 92/01923 and U.S. Pat. No. 6,353,226 documents.
However, none of the cited prior art documents provides or discloses a light reception system, and method, that allows simultaneously discriminating the light scattered from the surface of a sample of the light scattered from the inside of said sample. Therefore, the prior art solutions cannot capture and accurately distinguish the differentiated properties of either part of the sample.
To that end, present invention provides, according to one aspect, a light inspection system of the surface and inside of a sample, the system comprising:
According to the proposed system, said image of the sample is obtained, approximately, at a transversal plane with regard to the axis of each of the fibers of the bundle of bifurcated fibers. That is, a focal length of the parabolic mirror, the length from the parabolic mirror to the sample and the length from the parabolic mirror to the bundle of bifurcated fibers are selected such that the image of the sample is formed in the transversal plane of the fiber bundle input.
Therefore, the proposed system allows discriminating the light scattered from the surface of the sample of the light scattered from the inside of said sample.
Preferably, the parabolic mirror is concave and has a focal length of approximately 101 millimeters with a 90° configuration.
In an embodiment, the bundle of bifurcated fibers comprises a plurality of external fibers, preferably comprising at least three fibers, in an annular formation around said central fiber. The plurality of external fibers in this embodiment is located at a same distance from the central fiber.
In an embodiment, the measuring system includes a spectrometer and a power measuring device each one adapted for measuring said power and said spectrum in the central fiber and in the external fiber or the plurality of external fibers, independently, and also simultaneously (if considered necessary).
The light source may include any of: a supercontinuum laser light source comprising a light wavelength between 450 and 2400 nm; a visible enhanced supercontinuum source comprising a light wavelength below 450 nm; or a supercontinuum laser based in soft-glasses photonic crystal fibers providing emission ranges from 2 μm to 13 μm.
Besides, the light source may comprise a multiwavelength light source.
According to an embodiment, the system also includes one or more filters, located between the parabolic mirror and the sample, and configured to select a wavelength of the scattered light from the sample.
According to another embodiment, the imaging system further includes an optical device such as a galvanometric mirror or a polygonal rotating mirror, located between the parabolic mirror and the sample, and configured to change and orient the direction of the light beam towards the sample. The system may also include one or more filters, located between the light source and the optical device, and configured to excite the sample with light of arbitrary spectral shape.
Embodiments of the present invention also provide, according to another aspect, a light inspection method of the surface and inside of a sample. The method comprises generating, by a light source, a light beam having a broadband spectrum at a given optical power to impinge on a sample located at a given distance, said sample comprising a given translucency and absorption coefficients; obtaining, at a traversal plane of a bundle of bifurcated fibers, an image of said sample upon said impingement by an imaging system comprising a parabolic mirror with a hole, said parabolic mirror allowing the passage of the light beam towards the sample through said hole; redirecting, by the parabolic mirror, a scattered light component of the sample towards said bundle of bifurcated fibers, said bundle of bifurcated fibers being comprised of a central fiber and one external fiber or a plurality of external fibers; eliminating, by the parabolic mirror, the light coming from direct reflection at the sample through said hole; receiving scattered light from at least the surface of the sample by said central fiber of the bundle of bifurcated fibers; receiving scattered light exclusively from inside of the sample by said external fiber or by said plurality of external fibers of the bundle of bifurcated fibers; and measuring, by a measuring system, a power and/or a spectrum collected in the central fiber and in the external fiber or the plurality of external fibers, independently.
According to the proposed method, the sample may comprise any of a food such as a fruit or a vegetable, a recycling material or product, a wood material or product, a metal, a biological tissue, a textile, a plastic, a drug or medicine, a mineral, among others.
The previous and other advantages and features will be more fully understood from the following detailed description of embodiments, with reference to the attached figures, which must be considered in an illustrative and non-limiting manner, in which:
According to the preferred embodiment, the proposed system includes a light source 10 configured to generate a light beam with a broadband spectrum at a given optical power to impinge on the sample 1; an imaging system 20 configured to obtain an image of the sample upon said impingement; a light capturing unit comprising a bundle of bifurcated fibers 15 sustained by a holder 18, i.e. a number of fibers positioned side-by-side in a common end and split out into two legs at the other end (see
Preferably, the light source has multiple wavelengths. The light source 10 may be any of a supercontinuum laser light source having a light wavelength between 450 and 2400 nm, a visible enhanced supercontinuum source having a light wavelength below 450 nm or a supercontinuum laser based in soft-glasses photonic crystal fibers providing emission ranges from 2 μm to 13 μm. Even, the light source 10 could be a traditional broadband source such as a lamp, a superluminiscent diode (SLED), a white LED, etc. However, and because the power levels of the traditional broadband sources are lower supercontinuum light sources are preferred.
According to this preferred embodiment, the imaging system 20 comprises a parabolic mirror 21 with a hole and an optical device 22 such as galvanometric mirror or a polygonal rotating mirror capable to direct the light beam towards the sample 1. It should be noted that the optical device 22 is optional in the system architecture. That is, in alternative embodiments of the proposed system, in this case not illustrated, the imaging system 20 is formed only by the parabolic mirror 21.
The parabolic mirror 21 is adapted and configured such that it allows: the passage of the light beam towards the sample 1, the redirection of the scattered light component of the sample 1 towards the bundle of bifurcated fibers 15, and the elimination of the light coming from direct reflection at the sample 1. Preferably, the parabolic mirror 21 is concave and has a focal length of approximately 101 millimeters with a 90° configuration. The optical device 22 is adapted and configured to change and orient the direction of the light beam towards the sample 1.
Referring now to
The central fiber 17 is positioned to receive scattered light mainly from the surface of the sample 1. It should be noted that the central fiber 17 can also receive scattered light from the inside of the sample 1. The external fibers 16 are positioned to receive scattered light exclusively from inside the sample. Preferably, all the external fibers 16 of the bundle 15 are located at the same distance from the central fiber 17.
Referring back to
The system of
In another embodiment, the system may also comprise filters to select wavelength of the scattered light from the sample 1. For example, lowpass, bandpass and/or highpass filters, positioned between parabolic mirror 21 and fiber bundle 15. Also dispersive elements in these positions like prisms and diffractive gratings to discriminate wavelengths can be also used.
where l′ is the distance from the center of the parabolic mirror 21 to the collecting end of the fiber bundle 15, and l is the distance from the center of the parabolic mirror 21 to the surface of the sample 1 (following the optical path of the laser beam). The object plane corresponds to the plane which is tangent to the surface of the sample 1 and normal to the laser beam path.
With reference now to
The focal length of the parabolic mirror 21, the length/distance from the parabolic mirror 21 to the sample 1 and the length/distance from the parabolic mirror 21 to the bundle of bifurcated fibers 15 are selected such that the image of the sample 1 is formed in the transversal plane of the fiber bundle input.
While in the above description of the particular embodiments the sample has been indicated to be a food such as a fruit or a vegetable, the teachings of the invention are equally applicable to other types of samples such as recycling materials or products, wood materials or products, metals, a biological tissue, textiles, plastics, drugs or medicines, minerals, etc. this list not being limitative.
The scope of the present invention is defined in the following set of claims.
Number | Date | Country | Kind |
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17382427.7 | Jul 2017 | EP | regional |
Filing Document | Filing Date | Country | Kind |
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PCT/IB2018/054846 | 6/29/2018 | WO | 00 |