The present invention relates to a lighting device for performing small and medium scale microalgae growth experiments in research laboratories, as well as an incubator incorporating the lighting device for performing microalgae growth experiments.
In recent times, microalgae research has gained a lot of importance due to the different uses that can be given to them. Microalgae, among other applications, can be used to produce biofuels, pharmaceuticals, food, to treat wastewater or industrial water, for air conditioning and insulation of buildings, etc.
Microalgae cannot be harvested in large quantities directly from nature and must therefore be cultivated. For the different uses of microalgae to be economically viable, the production costs of microalgae need to be significantly reduced.
Currently, large-scale artificial cultivation of microalgae can be classified into open systems, mainly large ponds where microalgae are grown in the open air, and closed systems, where microalgae are grown inside photobioreactors, thus achieving a higher quality product. Regardless of the system employed, light is an essential factor for microalgae growth, and varies significantly with time of exposure to light, light intensity, and light spectrum. In open systems the light used is natural, and the low production rates are compensated by the large volumes of microalgae grown in the ponds, but in photobioreactors, which have a significantly smaller volume, an adequate artificial light source is required for microalgae growth.
The light demand required by microalgae for proper growth is much more complex than the light required by other types of plants, and varies significantly with each species of microalgae. For this reason, it is necessary to carry out small-scale experiments in research laboratories to determine the optimal conditions for the growth of each species of microalgae, so that these conditions can later be reproduced in photobioreactors on an industrial scale. Experiments are generally carried out with white light fluorescent lamps, and in some cases colored filters are used to modify the color of the light from the fluorescent lamps.
CN201746536U discloses a lighting device for performing microalgae growth experiments comprising a light source having light emitting diodes (leds) for illuminating the microalgae and a control unit configured to control the switching on and off of the leds and to vary the light intensity of the leds. The lighting device is arranged in a temperature-controlled microalgae incubator in which the microalgae are placed. The incubator has an upper shelf with two led light sources and a lower shelf with fluorescent lamps, so that the incubator allows the difference in growth of microalgae illuminated with leds to be tested against microalgae illuminated with conventional fluorescent lamps. The light source comprises a panel with 100 high-brightness leds in red, green, blue, yellow and white, and the control unit varies the on/off and light intensity of the leds to simulate the gradual change of sunlight.
Provided are a lighting device and an incubator for performing microalgae growth experiments.
An aspect of the invention relates to a lighting device for performing microalgae growth experiments comprising at least one light source having leds for illuminating the microalgae and a control unit configured to control the switching on and off of the leds and to vary the light intensity of the leds. The light source comprises RGB LEDs, each RGB LED has a housing that houses a red led, a green led and a blue led and a microcontroller that is connected to the control unit to vary the light intensity of the red led, the green led and the blue led of the RGB LED, such that the color and intensity of the light emitted by each RGB LED are modified by the control unit.
Another aspect of the invention relates to an incubator for performing microalgae growth experiments comprising shelves on which containers with the microalgae are placeable, at least one light source arranged on each shelf having leds to illuminate the microalgae, and a control unit configured to control the switching on and off of the leds and to vary the light intensity of the leds. The light source comprises RGB LEDs, each RGB LED has a housing that houses a red led, a green led and a blue led, and a microcontroller that is connected to the control unit to vary the light intensity of the red led, the green led and the blue led of the RGB LED, such that the color and intensity of the light emitted by each RGB LED are modified by the control unit.
The control unit allows each RGB LED to be controlled independently from the light source, so that the switch-on time, the light intensity and the type of color of each RGB LED can be modified. The RGB LEDs combine the primary colors red, green and blue to obtain any color, which allows the microalgae to be illuminated with the required color and intensity and for the required time, and therefore an infinite number of experiments can be carried out that can later be scaled up to an industrial level to cultivate microalgae in photobioreactors. For example, CN201746536U uses individual five-color leds, so it cannot change the color at the led level, which limits the experiments to the colors of the leds. Also, by using single-color led, if a single color wanted to be used, the leds of the other colors must be kept off, so CN201746536U uses high-brightness leds to achieve the required light intensity for microalgae growth.
These and other advantages and features of the invention will become apparent in view of the figures and the detailed description of the invention.
The invention relates to a lighting device for performing microalgae growth experiments comprising at least one light source 10 having leds 20 for illuminating the microalgae and a control unit 30 configured to control the switching on and off of the leds 20 and to vary the light intensity of the leds 20. See
The lighting device is used to illuminate microalgae strains in a controlled environment for small-scale experiments to optimize the production of the microalgae strain or its products of interest. The lighting device can be installed in temperature-controlled rooms, incubators (as shown in
The light source 10 comprises RGB LEDs (light emitting diodes) 20, wherein each RGB LED 20 has a housing that houses a red 21, green 22 and blue 23 led and a microcontroller 24 that is connected to the control unit 30 to vary the light intensity of the red 21, green 22 and blue 23 leds of the RGB LED 20, such that the color and intensity of the light emitted by each RGB LED 20 are modified by the control unit 30.
In this way, the lighting device has as many microcontrollers 24 as there are RGB LEDs 20 in the light source 10, so that the control unit 30 can independently select for each RGB LED 20 the combination of red, green and blue colors to obtain the required color, the light intensity of the RGB LED 20, and the switch-on time.
The lighting device has a power supply to electrically power the RGB LEDs 20 of the light source 10 and the control unit 30. The lighting device may have its own power supply, or a transformer and a conventional plug to connect to the grid. Preferably, the control unit 30 has the power supply integrated.
The frame 11 defines a housing in which the rear cover 12 with the RGB LEDs 20 and the front diffuser 13 are housed, providing rigidity to the light source 10 for its correct transport, fixing and use. The rear cover 12 that supports the RGB LEDs 20 is made of an opaque material to direct the light towards the front diffuser 13. The front diffuser 13 is made of a translucent material that allows the light to pass through, homogenizing it and directing it towards the microalgae.
As can be seen in the figures, the RGB LEDs 20 are arranged in led strips 27 on the front face of the rear cover 12. Alternatively, the rear cover 12 with the RGB LEDs may be a single element, such as an RGB LED panel, or an RGB LED panel that is arranged on the rear cover 12.
Preferably, the led strips 27 extend parallel to each other and are arranged horizontally on the rear cover 12, so that a homogeneous and compact distribution of the RGB LEDs 20 is obtained, allowing the light to be concentrated.
Preferably, the lighting device further comprises an atmospheric sensor 40 for measuring the pressure, temperature and humidity of the environment in which the lighting device is arranged. The atmospheric sensor 40 is employed by the control unit 30 to monitor the environment in which the microalgae cultivation experiment is performed and to check that the pressure, temperature and humidity conditions are in accordance with the experiment. The atmospheric sensor 40 is a single device that integrates a pressure sensor, a temperature sensor and a humidity sensor. Alternatively, separate sensors can be used to measure the pressure, temperature and humidity of the environment.
Preferably, the lighting device further comprises at least one light sensor 50 for measuring the light intensity of the environment in which the lighting device is arranged. The light sensor 50 may be a photoresistor or LDR having a resistance that varies as a function of the light incident on the sensor. The light sensor 50 is employed by the control unit 30 to check that the light source 10 is correctly emitting light, and the control unit 30 may have an alarm management system to alert the user when the light source 10 is not illuminated according to the required light intensity.
Even more preferably, the lighting device further comprises a wireless data transmitter and receiver 60 that is associated with an external electronic device 70 for sending and receiving data of the status and switching on of each RGB LED 20, data of the light intensity and color of each RGB LED 20, and for sending to the external electronic device 70 data of the light intensity, temperature, humidity, and pressure of the environment in which the lighting device is arranged. The wireless data transmitter and receiver 60 may be a modem with 4G connectivity.
The external electronic device 70 may be a computer, smartphone, tablet or the like with a custom-designed software application, through which the lighting device can be remotely controlled. The software application allows the parameterization of the light intensity and color of each RGB LED 20, and allows programming the switch-on time of the light source 10, as well as the display of variables such as the temperature, pressure and humidity measured by the atmospheric sensor 40, or the light intensity measured by the light sensor 50.
The lighting device described above may comprise several light sources 10 controlled by the control unit 30. For example, the light sources may be arranged in a temperature-controlled room where there is a microalgae culture, or they may be arranged in a laboratory incubator as depicted in
Accordingly, the invention also relates to an incubator 100 for performing microalgae growth experiments comprising shelves 110 on which containers 120 with the microalgae are placeable, at least one light source 10 arranged on each shelf 110 having leds 20 to illuminate the microalgae, and a control unit 30 configured to control the switching on and off of the leds 20 and to vary the light intensity of the leds 20. The light source 10 comprises RGB LEDs 20, wherein each RGB LED 20 has a housing that houses a red led 21, a green led 22 and blue led 23 and a microcontroller 24 that is connected to the control unit 30 for varying the light intensity of the red led 21, the green led 22 and the blue led 23 of the RGB LED 20, such that the color and intensity of the light emitted by each RGB LED 20 are modified by the control unit 30.
Preferably, on each shelf 110 are arranged lines 130 of containers 120, each line 130 having a left side and a right side, and each shelf 110 has rows 140 of light sources 10, with at least one light source 10 per row 140, and at least one side of each line 130 of containers 110 is illuminated by a respective row 140 of light sources 10. This ensures that all containers 120 on each shelf 110 are directly illuminated and that there are no containers 120 shading others.
As shown in
Each light source 10 of the incubator has several RGB LEDs 20, and each RGB LED 20 has a housing that houses a red led 21, a green led 22 and a blue led 23, and a microcontroller 24 that is connected to the control unit 30 to vary the light intensity of the red led 21, the green led 22 and the blue led 23 of the RGB LED 20. The control unit 30 has power cables 31 and 32 for powering the light sources and a data bus 33 which is connected to the microcontroller 24 of each RGB LED 20 to control the timing and switching on of the RGB LEDs 20, their light intensity and color type.
The incubator 100 comprises an atmospheric sensor 40 for measuring the pressure, temperature and humidity inside the incubator where the microalgae are available, and the control unit 30 is operatively connected with the atmospheric sensor 40.
The incubator 100 has a light sensor 50 on each shelf 110 to measure the intensity of each shelf 110 of the incubator, and the control unit 30 is operatively connected with the light sensors 50 to check that the light sources 10 on each shelf 110 are operating within the parameters required by the experiment.
The incubator further comprises a wireless data transmitter and receiver 60 that is associated with an external electronic device 70 for sending and receiving data of the status and power on of each RGB LED 20, data of the light intensity and color of each RGB LED 20, and for sending to the external electronic device 70 data of the light intensity, temperature, humidity, and pressure of the incubator in which the microalgae are available.
Specifically, the phycobiliproteins of a cyanobacterial strain (phycocyanin PC, allophycocyanin APC and phycoerythrin PE), as well as the biomass of the cyanobacterial strain, were exposed to white, red and the combination of red and blue light. As can be seen in the figure, the color used has a great influence on relevant parameters in terms of microalgae production. It can be seen how white and red light achieved the best pigment and biomass production values in the strain studied. Accordingly, given these results, when cultivating this strain for industrial exploitation, the color of the led source chosen for the photobioreactor would be red due to its lower energy consumption and its good results when producing the products of interest.
In following clauses additional embodiments are disclosed.
Clause 1. Lighting device for performing microalgae growth experiments, comprising at least one light source (10) having leds (20) for illuminating the microalgae and a control unit (30) configured to control the switching on and off of the leds (20) and to vary the light intensity of the leds (20), the light source (10) comprises RGB LEDs (20), and in that each RGB led (20) has a housing that houses a red led (21), a green led (22) and a blue led (23), and a microcontroller (24) that is connected to the control unit (30) to vary the light intensity of the red led (21), the green led (22) and the blue led (23) of the RGB LED (20), such that the color and intensity of the light emitted by each RGB led (20) are modified by the control unit (30).
Clause 2. Lighting device according to clause 1, wherein the light source (10) has a frame (11) supporting a rear cover (12) and a front diffuser (13), the rear cover (12) has a front face on which the RGB LEDs (20) are arranged and the front diffuser (13) is arranged on the front face of the rear cover (12) to homogenize the light emitted by the RGB LEDs (20).
Clause 3. Lighting device according to clause 2, wherein the RGB LEDs (20) are arranged in led strips (27) on the front face of the rear cover (12).
Clause 4. Lighting device according to the preceding clause, wherein the led strips (27) extend parallel to each other and are arranged horizontally on the rear cover (12).
Clause 5. Lighting device according to any one of clauses 1 to 4, further comprising an atmospheric sensor (40) for measuring the pressure, temperature and humidity of the environment in which the lighting device is arranged.
Clause 6. Lighting device according to any one of the preceding clauses, further comprising at least one light sensor (50) for measuring the light intensity of the environment in which the lighting device is arranged.
Clause 7. Lighting device according to the preceding clause, further comprising a wireless data transmitter and receiver (60) that is associated with an external electronic device (70) for sending and receiving data of the status and switching on of each RGB LED (20), data of the light intensity and color of each RGB LED (20), and for sending to the external electronic device (70) data of the light intensity, temperature, humidity, and pressure of the environment in which the lighting device is arranged.
Clause 8. Incubator for performing microalgae growth experiments, comprising shelves (110) on which containers (120) with the microalgae are placeable, at least one light source (10) arranged on each shelf (110) having leds (20) to illuminate the microalgae, and a control unit (30) configured to control the switching on and off of the leds (20) and to vary the light intensity of the leds (20), the light source (10) comprises RGB LEDs (20), and in that each RGB LED (20) has a housing that houses a red led (21), a green led (22) and a blue led (23), and a microcontroller (24) that is connected to the control unit (30) to vary the light intensity of the red led (21), the green led (22) and the blue led (23) of the RGB LED (20), such that the color and intensity of the light emitted by each RGB LED (20) are modified by the control unit (30).
Clause 9. Incubator according to the preceding clause, wherein the light source (10) has a frame (11) supporting a rear cover (12) and a front diffuser (13), the rear cover (12) has a front face on which the RGB LEDs (20) are arranged and the front diffuser (13) is arranged on the front face of the rear cover (12) to homogenize the light emitted by the RGB LEDs (20).
Clause 10. Incubator according to the preceding clause, wherein the RGB LEDs (20) are arranged in led strips (27) on the front face of the rear cover (12).
Clause 11. Incubator according to the preceding clause, wherein the led strips (27) extend parallel to each other and are arranged horizontally on the rear cover (12).
Clause 12. Incubator according to any one of clauses 8 to 11, further comprising an atmospheric sensor (40) for measuring the pressure, temperature and humidity inside the incubator where microalgae are placeable.
Clause 13. Incubator according to any one of clauses 8 to 12, further comprising a light sensor (50) on each shelf (110) for measuring the intensity of each shelf (110) of the incubator.
Clause 14. Incubator according to the preceding clause, further comprising a wireless data transmitter and receiver (60) which is associated with an external electronic device (70) for sending and receiving data of the status and switching on of each RGB LED (20), data of the light intensity and color of each RGB LED (20), and for sending to the external electronic device (70) data of the light intensity, temperature, humidity, and pressure of the incubator where the microalgae are placeable.
Clause 15. Incubator according to any one of clauses 8 to 14, wherein on each shelf (110) are arranged lines (130) of containers (120), each line (130) having a left side and a right side, and each shelf (110) has rows (140) of light sources (10), with at least one light source (10) for each row (140), and wherein at least one side of each row (130) of containers (120) is illuminated by a respective row (140) of light sources (10).
Clause 16. Incubator according to the preceding clause, wherein both left and right sides of each line (130) of containers (120) are illuminated by a respective row (140) of light sources (10).
Number | Date | Country | Kind |
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P202130614 | Jun 2021 | ES | national |
This application relates to and claims the benefit and priority to International Application No. PCT/ES2022/070416, filed Jun. 29, 2022, which claims the benefit and priority to Spanish Application No. P202130614, filed Jun. 30, 2021, each of which is incorporated herein by reference in its entirety.
Number | Date | Country | |
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Parent | PCT/ES2022/070416 | Jun 2022 | US |
Child | 18398722 | US |