The present invention relates in general to the processing of biological samples, and in particular to isolating small portions of a single initial biological sample.
It is generally desirable to be able to obtain small quantities of a biological sample from an initial material, such as whale blubber, for various experimental purposes. In addition to obtaining precise small quantities, the material obtained must be grinded to a powder. Such grinding is typically accomplished by placing the sample into a tubular chamber, similar to a test tube, along with a ball or other striking member inside. Movement of the tubular chamber then grinds the sample.
When plural tubular chambers are used, the grinding process must be substantially identical among the chambers. One challenge that scientists face is that there exists no known apparatus for ensuring that all of the plurality of tubular chambers is oscillated in precisely the same manner.
Accordingly, there is a need in the art for improved apparatuses and methods for grinding a plurality of samples by oscillating plural tubular members simultaneously, and ensuring substantially uniform oscillatory movement and grinding among the tubular members.
According to one aspect, the present invention is directed to a sample container motion device, that may include a guide having a main channel, the main channel including at least one bearing channel; a carriage configured to move within the main channel of the guide, the carriage having at least one bearing groove corresponding to the at least one bearing channel; a slide system configured to enable linear movement between the carriage and the guide, the slide system including at least one bearing guide located in between each bearing groove and its corresponding bearing channel; wherein each bearing guide may include a plurality of ball bearings; a ball spacer having openings for the ball bearings and configured to confine centers of the ball bearings in a fixed relation to a structure of the spacer.
Other aspects, features, advantages, etc. will become apparent to one skilled in the art when the description of the preferred embodiments of the invention herein is taken in conjunction with the accompanying drawings.
For the purposes of illustrating the various aspects of the invention, there are shown in the drawings forms that are presently preferred, it being understood, however, that the invention is not limited to the precise arrangements and instrumentalities shown.
In the following description, for purposes of explanation, specific numbers, materials and configurations are set forth in order to provide a thorough understanding of the invention. It will be apparent, however, to one having ordinary skill in the art that the invention may be practiced without these specific details. In some instances, well-known features may be omitted or simplified so as not to obscure the present invention. Furthermore, reference in the specification to phrases such as “one embodiment” or “an embodiment” means that a particular feature, structure or characteristic described in connection with the embodiment is included in at least one embodiment of the invention. The appearances of phrases such as “in one embodiment” or “in an embodiment” in various places in the specification do not necessarily all refer to the same embodiment.
Motion device 100 may include guideway 200, carriage 300, and bearing guides 400. Guideway 200 may include main channel 206 (
In one embodiment, a material sample may be placed in carriage 300. Thereafter, carriage 300 may be moved back and forth with respect to guideway 200 while being moved by an electric motor or other suitable power source such as but not limited to pneumatic, hydraulic, or other suitable power mechanism.
Isolating samples of biological or other material may involve cycling carriage 300 back and forth within guideway 200 a very large number of times, which may include hundreds of thousands or even millions of cycles. Accordingly, embodiments of the present invention benefit from stability, consistency, and linearity of the movement of carriage 300 with respect to guideway (guide) 200. Various features of the embodiments of motion device 100 discussed herein are operable to provide the above-listed characteristics.
Directing attention to
In one embodiment, each bearing assembly 500 may include a bearing guide 400 having a ball spacer 402 and a plurality of ball bearings 406 in between a bearing groove 302 of carriage 300 and a bearing channel 202 of guide 200. Ball bearings 206 may experience rolling contact with rods disposed along the lengths of the carriage bearing groove 302 and the guide bearing channel 202. Bearing assemblies 500 are discussed in greater detail below in connection with
Guide 200 may include a plurality of channels to enable the relative motion of carriage 300 with respect to guide 200. In this embodiment, guide 200 may include main channel 206 (which is best shown in
Motion device 100 preferably includes multiple bearing assemblies 500 (see
The distribution of bearing assemblies 500 in motion system 100 is preferably selected to enable optimal stability and to minimize rolling resistance. In this embodiment, four bearing assemblies 500 are deployed. One set of bearing assemblies 500 is deployed on each side (an upper side and a lower side in the view of
Guide 200 may include a plurality of bearing channels 202-A, 202-B, 202-C, and 202-D to bear the force of contact between guide 200 and to house various parts suitable for optimizing characteristics of the bearing interface between guide 200 and carriage 300. Each bearing channel 202 preferably includes a spacer bar 208 and two rods 204. Rods 204 preferably provide surfaces that ball bearings 406 of bearing guides 400 roll along to enable to the movement of carriage 300 with respect to guide 200. The spacer bar 208 may be optimally constructed from rubber or similar material with slight malleability to provide a slight “give.” The slight “give” is desired to prevent the bearings from carrying the entire force. The “give” allows some space for the system to absorb the force and permits the bearings to roll without grinding.
Herein, preferred dimensions for the parts forming guide 200, carriage 300, and bearing guide 400 are listed. While the listed dimensions are preferred, it will be appreciated that parts having dimensions (whether widths, length, thickness, or diameters) greater than or smaller than those listed may be employed, and that all such variations are intended to be included within the scope of the invention. In one embodiment, guide 200 may have a length of about 4.8 inches, and a width of about 2.75 inches. Main channel 206 may have a width of about 1.2 inches not counting the depth of the bearing channels 202. Each bearing channel 202 may have a width of about 0.368 inches and a depth of about 0.255 inches. Guide 200 may be made of anodized aluminum, but is not limited to this material. Any other suitable metal or suitable non-metallic material may be employed.
Rods 204 may have a diameter of about 0.125 inches. Spacer bar 208 may have a thickness of about 0.03 inches. Rods 204, rods 304, and spacer bar 208 may be made of stainless steel, however other materials may be employed in place of, or in addition to stainless steel for one or more of the listed parts.
Carriage 300 preferably includes a plurality of bearing grooves 302-A, 302-B, 302-C, 302-D having locations along the perimeter of carriage 300 and dimensions corresponding to the locations and dimensions, respectively, of bearing channels 202 of guide 200. Each bearing groove 302 may include rods 304 which preferably experience rolling contact with ball bearings 406 of bearing guides 400.
In one embodiment, carriage 300 may have a length (the dimension from left to right in the view of
With reference to
Each bearing assembly 500 is formed by parts belonging to guide 200, carriage 300, and bearing guide 400.
A case is considered in which guide 200 is stationary, and in which carriage 300 moves “into the page” in the views of
Employing a motion device in accordance with the embodiments described above preferably provides highly stable, repeatable, and accurate motion of carriage 300 with respect to guide 200. Moreover, the above-described configuration is preferably able to enable very large numbers of cycles to be practiced with minimal wear on the various bearing surfaces.
Although the invention herein has been described with reference to particular embodiments, it is to be understood that these embodiments are merely illustrative of the principles and applications of the present invention. It is therefore to be understood that numerous modifications may be made to the illustrative embodiments and that other arrangements may be devised without departing from the spirit and scope of the present invention as defined by the appended claims.