BRIEF DESCRIPTION OF THE DRAWINGS
The present invention will become apparent upon reading of the following detailed description of the present invention in conjunction with the drawings, as follows:
FIG.1 is a diagram of the stability analysis of different liposomes stored at 4° C. according to the present invention; DSPC: Egg PC: Cholesterol: PEG-DSPE=8:2:1:0.2 μmole, (▪) 9:1:1:0.2 μmole, (▴) 10:0:1:0.2 μmole, (×) 10:0:2:0.2 μmole, (*) 10:0:3:0.2 μmole.
FIG. 2 is a diagram of drug release from the liposome in 37° C. PBS after light activating, the encapsulated drug is Hp.
FIG. 3 is a diagram of drug release from the liposome in 37° C. PBS after light activating, the encapsulated drug is PpIX.
FIG. 4 is a diagram of the lipid bilayer permeability change of the liposome in the absence of photosensitizer after 30 J/cm2 light irradiation, the model drug used to monitor the permeability of the membrane is Doxorubicin.
FIG. 5 is a diagram of the lipid bilayer permeability change of the liposome in the absence of photosensitizer after 30 J/cm2 light irradiation, the model drug used to monitor the permeability of the membrane is Calcein.
FIG. 6 is a diagram of the lipid bilayer permeability change of the liposome encapsulating a photosensitizer (Hp) after 30 J/cm2 light irradiation, the model drug used to monitor the permeability of the membrane is Doxorubicin.
FIG. 7 is a diagram of the lipid bilayer permeability change of the liposome encapsulating a photosensitizer (Hp) after 30 J/cm2 light irradiation, the model drug used to monitor the permeability of the membrane is Calcein;
FIG. 8 is a diagram of the toxicity of various formulation to CL1-0 cell Liposomal-Hp and free-Hp were added into the cell culture system and light irradiation was provided with doses from 0 J, 2 J, 4 J, 6 J, to 8 J;
FIG. 9 is a diagram of the toxicity of various formulation to A431 cells, Liposomal Doxorubicin (LD), Free Doxorubicin (FD), Liposomal-Dox-Hp (LDH),
Liposomal Hp (LH), and LDH treating with 1 J, 10 J, 20 J, and 30 J of light were compared;
FIG. 10 is a diagram of the toxicity of various formulation to CL1-0 cells.
Liposomal Doxorubicin (LD), Free Doxorubicin (FD), Liposomal-Dox-Hp (LDH), and LDH treated with 1 J, and 10 J of light were compared.