Claims
- 1. A liquid affinity support containing an attached ligand or binder for the ligand consisting essentially of:
- (a) a chemically inert, water immiscible liquid perfluorocarbon carrier having specific gravity greater than that of water and further having low nonspecific binding to a ligand or binder for the ligand; and
- (b) a perfluorocarbon-substituted ligand or binder for the ligand securely but reversibly attached to the surface of said carrier through a highly fluorinated isocyanate anchor group.
- 2. The liquid affinity support of claim 1, wherein said ligand is a nucleic acid.
- 3. The liquid support of claim 1 wherein the highly fluorinated isocyanate anchor group is a compound of the formula:
- R.sub.F --CH.sub.2 CH.sub.2 CH.sub.2 NCO
- wherein R.sub.F is a linear, branched or cyclic perfluorinated radical containing 1-20 carbon atoms.
- 4. The liquid support of claim 3 wherein R.sub.F is F(CH.sub.2).sub.8.
- 5. The support of claim 1, wherein said ligand is an antigen.
- 6. The support of claim 1, wherein said ligand is a hapten.
- 7. The liquid affinity support of claim 1, wherein said ligand is an enzyme substrate.
- 8. The liquid affinity support of claim 1, wherein said ligand is an enzyme inhibitor.
CROSS REFERENCE TO RELATED APPLICATION
This is a continuation of application Ser. No. 07/134,026, filed Dec. 17, 1987, abandoned which is a continuation-in-part of an application entitled Bioaffinity Separations with Liquid Affinity Supports, Ser. No. 07/032,642, filed Mar. 31, 1987 now abandoned, which is a continuation-in-part of Ser. No. 06/863,607, filed May 15, 1986 now abandoned, both by Julian P. Breillatt, Jr. et al.
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Entry |
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Lehninger, Biochemistry (1970) Worth Publishers, N.Y., N.Y. pp. 150-165. |
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Continuations (1)
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Date |
Country |
Parent |
134026 |
Dec 1987 |
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Continuation in Parts (2)
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Number |
Date |
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Parent |
32642 |
Mar 1987 |
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Parent |
863607 |
May 1986 |
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