Patent Grant
10465170
The instant application contains a Sequence Listing which has been submitted via EFS-web and is hereby incorporated by reference in its entirety. The ASCII copy, created on Feb. 18, 2016, is named RINCH02WOU1_SL.txt, and is 15,400 bytes in size.
The pneumoviruses include many important human and animal pathogens, including human respiratory syncytial virus (hRSV), bovine RSV, human metapneumovirus (hMPV), avian MPV, and pneumovirus of mice. Among these viruses, hRSV and hMPV are the leading causes of acute respiratory tract infection in infants and children. Despite major efforts, there is no antiviral or vaccine to help combat these diseases.
First discovered in 1956 as a lower respiratory tract pathogen of children in their first year of life, human respiratory syncytial virus is an enveloped, negative-sense single-stranded RNA virus belonging to the Pneumovirus genus within the Pneumovirinae subfamily of the family Paramyxoviridae. hRSV is a major cause of lower respiratory tract infections, most commonly resulting in mild respiratory tract disease. However, infection with hRSV may result in severe bronchiolitis and pneumonia. In industrialized countries, hRSV accounts for up to 70% of hospitalized bronchiolitis cases. Worldwide, hRSV is estimated to cause 199,000 deaths annually in children less than 5 years.
Bovine respiratory syncytial virus (bRSV) is closely related to hRSV, although there is no evidence that cross-species transmission between cattle and humans occurs. bRSV is considered a component of the bovine respiratory disease complex, and infects cattle of all ages, although suckling calves often experience the most severe disease. Serologic surveys indicate that bRSV is a very common virus in cattle populations worldwide.
Human metapneumovirus, first characterized in 2001 in the Netherlands, belongs to the Metapneumovirus genus within the Pneumovirinae subfamily of the family Paramyxoviridae. Soon after its discovery, hMPV was recognized as a globally prevalent pathogen, likely having been causing respiratory illnesses for at least 50 years worldwide. A negative-sense single-stranded RNA virus, hMPV has been isolated from individuals of all ages with acute respiratory tract infection, especially in infants, children, the elderly, and immunocompromised individuals. hMPV infection is recognized as a leading cause of respiratory tract infection in the first years of life with symptoms similar to that of hRSV infection, including mild respiratory problems to severe coughs, bronchiolitis, and pneumonia. Transmission likely occurs through contact with contaminated secretions, and via droplet, aerosol, or fomite vectors. Epidemiological studies suggest that 5 to 15% of all respiratory tract infection in infants and young children are caused by hMPV, a proportion second only to that of hRSV.
The only other member in the genus Metapneumovirus is avian metapneumovirus (aMPV), also known as avian pneumovirus or Turkey Rhinotracheitis, is an economically important pathogen that causes acute respiratory disease in turkeys, and has been associated with swollen head syndrome in broiler chicken breeds as well as egg production losses in laying chicken breeds. First detected in turkeys in South Africa in the late 1970s, aMPV has since spread to all major poultry-producing areas in the world, except for Australia. In addition to affecting turkeys and chickens, aMPV has been detected in pheasants, Muscovy ducks, and guinea fowl. Epidemiologic studies provide evidence for the circulation of aMPV in wild birds, particularly water-associated species. Experimental studies have shown that turkeys may also be susceptible to hMPV. Infection with aMPV is often complicated by secondary bacterial infections, leading to high economic losses.
The genome of hMPV is a non-segmented negative-sense (NNS) RNA, with size ranging from 13,280 to 13,378 nucleotides, and contains 8 genes which encode for 9 proteins in the order of 3′-N-P-M-F-M2-SH-G-L-5′. Like all NNS RNA viruses, the genomic RNA is completely encapsulated by nucleoprotein (N), forming the N-RNA complex that serves as a template for genome replication and mRNA transcription. During replication, the RNA dependent RNA polymerase (RdRp) enters at the extreme 3′ end of the genome and synthesizes full-length complementary antigenome, which in turn serves as template for synthesis of full-length progeny genome. During transcription, RdRp copies the genomic RNA template to synthesize a short uncapped leader RNA, and capped, methylated, and polyadenylated mRNAs that encode all viral proteins. The components of RdRp of Paramyxovirinae subfamily (family Paramyxoviridae), Rhabdoviridae, and Bornaviridae include the large (L) protein catalytic subunit and phosphoprotein (P) cofactor. The RdRp of the Pneumovirinae subfamily of the family Paramyxoviridae requires the M2-1 protein as an additional cofactor, whereas the Filoviridae (such as Ebola virus and Marburg virus) polymerase requires VP30 as an additional cofactor. Both the M2-1 protein of pneumoviruses and VP30 protein of filoviruses are typical zinc binding proteins thought to play many critical regulatory roles in RNA synthesis and processing via poorly understood mechanisms.
Metal ions were shown to be integrated in several gene regulatory proteins as early as the 1970s. Among them, zinc is an important structural component of proteins involved in nucleic acid binding and gene regulation. Zinc binding motifs, including CCHH, CCHC and CCCH, are often involved in transcriptional and translational processes. Many viruses encode their own zinc binding proteins that regulate viral replication and/or pathogenesis, such as NS5A protein of hepatitis C, almost all retroviral nucleocapsid proteins, which comprise one or two copies of a zinc finger motif essential for viral replication, the V proteins of many paramyxoviruses, and the VP30 protein of Ebola.
The M2-1 protein is unique to all known pneumoviruses. The current understanding of the functions of M2-1 proteins comes predominantly from studies of the hRSV M2-1 protein. The hRSV M2-1 functions as a transcriptional elongation factor and anti-terminator that enhances read-through of intergenic junctions. Thus, M2-1 is essential for synthesis of full-length mRNAs and polycistronic mRNAs. The hRSV M2-1 was found to be an RNA binding protein, although its RNA binding specificity is controversial. Recent NMR studies showed that the RSV M2-1 core domain preferentially recognizes poly-A tails of viral mRNAs. M2-1 likely binds nascent mRNA transcripts, preventing premature termination through stabilization of the transcription complex and inhibition of RNA secondary structure formation. Additionally, hRSV M2-1 was shown to interact with the N, P, and L proteins.
Described herein are mutant pneumoviruses comprising a mutated zinc binding motif in an M2-1 protein of the pneumovirus, wherein at least one amino acid of the zinc binding motif is mutated relative to wild-type pneumovirus. The mutant pneumoviruses described herein grow to high titer in cell culture, are genetically stable, are attenuated in vitro and in vivo, and are highly immunogenic. Also described herein are vaccine compositions, including vaccines, comprising the live attenuated mutant pneumoviruses provided herein. Vaccine compositions described herein may further comprise a pharmaceutically acceptable carrier, vehicle, excipient, and/or adjuvant. Methods for inducing a protective immune response in a subject against a pneumovirus infection are also described and disclosed. The vaccine compositions and methods described herein can be used to prevent metapneumovirus and respiratory syncytial virus infection in humans, respiratory syncytial virus infection in cattle, avian metapneumovirus infection in various avian species, and pneumonia virus of mice in rodents.
In a particular embodiment described herein is a mutant pneumovirus comprising a mutated zinc binding domain in an M2-1 protein of the pneumovirus, wherein at least one amino acid of the zinc binding domain is mutated relative to wildtype pneumovirus. In certain embodiments, the amino acid mutation in the zinc binding domain is non-lethal and at least partially inhibits zinc binding activity of the M2-1 protein or abolishes zinc binding activity of the M2-1 protein. In certain embodiments, the at least one amino acid mutation occurs at an amino acid selected from the group consisting of C7, C15, C21, and H25.
In another embodiment described herein is a vaccine composition comprising a mutant pneumovirus described herein. In certain embodiments, the vaccine composition is a live attenuate vaccine.
In yet another particular embodiment described herein is a method for inducing a protective immune response in a subject, comprising administering to the subject an immunologically effective dose of a vaccine composition described and disclosed herein.
In still another particular embodiment described herein is a method for preparing a vaccine composition described and disclosed herein comprising mixing a mutant pneumovirus described and disclosed herein with at least one of a pharmaceutically acceptable carrier, a pharmaceutically acceptable vehicle, a pharmaceutically acceptable excipient, and a pharmaceutically acceptable adjuvant. In certain embodiments, the vaccine composition is formulated for administration via an administration route selected from the group consisting of intranasal administration, subcutaneous administration, intramuscular administration, intradermal administration, and oral administration.
In another embodiment described herein is a kit comprising a vaccine composition described and disclosed herein and at least one container.
The patent or application file may contain one or more drawings executed in color and/or one or more photographs.
SEQ ID NO: 1 discloses the amino acid sequence of human metapneumovirus subtype A (hMPV-A; gi: 215794520).
SEQ ID NO: 2 discloses the amino acid sequence of human metapneumovirus subtype B (hMPV-B; gi: 215794505).
SEQ ID NO: 3 discloses the amino acid sequence of avian metapneumovirus subtype A (aMPV-A; gi: 49823139).
SEQ ID NO: 4 discloses the amino acid sequence of avian metapneumovirus subtype B (aMPV-B; gi: 310772463).
SEQ ID NO: 5 discloses the amino acid sequence of avian metapneumovirus subtype C (aMPV-C; gi: 237847064).
SEQ ID NO: 6 discloses the amino acid sequence of human respiratory syncytial virus type A (hRSV-A; gi: 333959).
SEQ ID NO: 7 discloses the amino acid sequence of human respiratory syncytial virus type B (hRSV-B; gi: 2582031).
SEQ ID NO: 8 discloses the amino acid sequence of bovine respiratory syncytial virus (BRSV; gi: 210823).
SEQ ID NO: 9 discloses the amino acid sequence of pneumonia virus of mice (PVM; gi: 56900724).
Throughout this disclosure, various publications, patents and published patent specifications are referenced. The disclosures of these publications, patents and published patent specifications are hereby incorporated by reference into the present disclosure to more fully describe the state of the art to which this invention pertains.
In particular embodiments described herein are mutated pneumoviruses, wherein the pneumovirus is mutated relative to a comparable wild-type pneumovirus. In certain embodiments, the mutated pneumovirus is recoverable. The mutated pneumovirus can be any pneumovirus comprising a CCCH zinc binding motif, including but not limited to human metapneumovirus subtype A (hMPV-A), human metapneumovirus subtype B (hMPV-B), avian metapneumovirus subtype A (aMPV-A), avian metapneumovirus subtype B (aMPV-B), avian metapneumovirus subtype C (aMPV-C), avian metapneumovirus subtype D (aMPV-D), human respiratory syncytial virus type A (hRSV-A), human respiratory syncytial virus type B (hRSV-B), bovine respiratory syncytial virus (BRSV), and pneumonia virus of mice (PVM). In certain embodiments, the mutated pneumovirus is a mutated hMPV, a mutated hRSV, or a mutated aMPV.
The recoverable mutated pneumovirus is attenuated in vivo. The mutated pneumovirus, or an immunogenic part thereof, can be used as a pneumovirus vaccine. Vaccines described herein can be utilized to induce a protective immune response in a subject against future viral challenge.
General Description
Using highly purified human metapneumovirus (hMPV) M2-1 protein expressed from E.coli, the studies described herein showed that hMPV M2-1 is a zinc binding protein that coordinates zinc ions at a molecular ratio of 1:1. Subsequent mutagenesis showed that C21 and H25 in the zinc binding motif are essential for efficient zinc binding activity, whereas C7 and C15 play more minor roles in zinc binding. Recombinant hMPVs (rhMPVs) lacking zinc binding activity were not only highly attenuated in replication in cell culture and in cotton rats, but also elicited high level of neutralizing antibody and provided protection against viral challenge by rhMPV. In contrast, rhMPV mutants retaining approximately 60% of zinc binding activity replicated as efficiently as wild-type (wt) rhMPV in vitro and in vivo. Collectively, these data indicate that the zinc binding activity of M2-1 is indispensable for hMPV viral replication and pathogenesis in vivo.
The studies described herein further showed that hRSV M2-1 binds and coordinates zinc ion through a CCCH motif at a molecular ratio of 1:1, similarly to the hMPV M2-1 protein. Mutagenesis showed that mutations at C7, C21, and H25 decreased hRSV M2-1 zinc binding by approximately 40%, while a mutation at C15 decreased zinc binding by approximately 20%. Unlike in previous studies where amino acid substitutions (C to G) in the first three cysteine residues (C7, C15, and C21) were lethal to RSV (Tang R S et al., 2001. Requirement of cysteines and length of the human respiratory syncytial virus M2-1 protein function and virus availability. J. Virol. 75:11328-35), the C7S, C15S, C21S, and H25L hRSV M2-1 mutants described herein were all recoverable. Furthermore, the C21S and H25L mutants described herein were shown to be attenuated in vitro.
As shown in
Recently, the crystal structures of both the hRSV and hMPV M2-1 proteins were solved (Tanner S J et al., 2014. Crystal structure of the essential transcription antiterminator M2-1 protein of human respiratory syncytial virus and implications of its phosphorylation. PNAS USA, 111:1580-85) (see
As described in the Examples below, the last two amino acid residues in the zinc binding motif in hMPV M2-1 (C21 and H25) are essential for zinc binding activity and that the amino acids in the zinc binding motif are essential for oligomerization of M2-1 protein. Unlike reports for RSV, rhMPVs lacking zinc binding activity were successfully recovered from infectious cDNA clones. rhMPVs lacking zinc binding activity were not only attenuated in vitro cell culture system, but also attenuated in viral replication in upper and lower respiratory tract of cotton rats. Unlike rhMPV lacking the entire M2-1 gene, cotton rats immunized with rhMPVs lacking zinc binding activity triggered a high level of neutralizing antibody and were completely protected from viral challenge with wild-type rhMPV.
Also described in the Examples below, and contrary to previous reports, is the recoverability of rhRSV comprising single amino acid mutations in the zinc binding motif. Whereas it was previously reported that single amino acid substitutions in the first three cysteine residues (namely C7G, C15G, and C21G) were lethal to hRSV thus preventing recovery of the virus (Tang R S et al., 2001. Requirement of cysteines and length of the human respiratory syncytial virus M2-1 protein function and virus availability. J. Virol. 75:11328-35), recombinant RSV-GFP (rgRSV) comprising a single amino acid mutation in the zinc binding motif (C7S, C15S, C21S, or H25L) was recoverable. Additionally, rgRSV-C21S and H25L were attenuated in vitro. In certain embodiments, an attenuated recombinant pneumovirus with at least one amino acid mutation at C7, C15, C21 or H25 is provided. In one particular embodiment, the at least one amino acid mutation is any amino acid mutation that attenuates the pneumovirus and allows for recovery of the recombinant pneumovirus. In another particular embodiment, the at least one amino acid is at least one of C7S, C15S, C21S, and H25L. In yet another particular embodiment, the at least one amino acid mutation is not C7G, C15G, or C21G.
Techniques for introducing mutations into nucleic acids encoding, for example, the pneumovirus M2-1 protein, are well-known to the skilled person and include, for example, but without limitation site-directed mutagenesis by PCR, homologous recombination, restriction enzyme digestion, ligation, CRISPR/Cas-9 etc. Standard reference works setting forth the general principles of recombinant DNA technology include Molecular Cloning: A Laboratory Manual, 2nd ed., vol. 1-3, ed. Sambrook et al., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989; Current Protocols in Molecular Biology, ed. Ausubel et al., Greene Publishing and Wiley-Interscience, New York, 1992 (with periodic updates); Innis et al., PCR Protocols: A Guide to Methods and Applications, Academic Press: San Diego, 1990. For further description of CRISPR/Cas-9, see US20100076057A1, WO2010075424A2, WO2013126794A1, WO2013142578, WO2013169398, WO2013176772A1, US2013181440A1, US20140017214A1, WO2014011237A1, WO2014022702A2, WO2014071219A1, U.S. Pat. No. 8,697,359B1, and WO2014018423A2.
In certain embodiments wherein the recombinant pneumovirus is either a metapneumovirus or a respiratory syncytial virus, a virus comprising a mutated zinc binding domain in the M2-1 protein resulting in partially inhibited zinc binding activity will be recoverable. In other embodiments wherein the recombinant pneumovirus is a metapneumovirus, a virus comprising mutated zinc binding domain in the M2-1 protein resulting in abolished zinc binding activity will be recoverable. For metapneumoviruses, the amino acids of the CCCH binding motif can be substituted by any other amino acid that results in partially inhibited or abolished zinc binding activity. For respiratory syncytial virus, a moderate substitution, such as cysteine to serine (differs at a single atom—the sulfur of cysteine thiol is replaced by the serine oxygen in an alcohol), will partially inhibit the zinc binding activity of the M2-1 protein, resulting in an attenuated but recoverable virus. Other possible substitutions for cysteine residues of the CCCH zinc binding domain of a respiratory syncytial virus M2-1 protein include alanine, threonine, and methionine, while the histidine residue can be substituted by leucine, lysine, arginine, valine, isoleucine, methionine, or proline, as well as others.
In certain embodiments where the pneumovirus is a human metapneumovirus, the pneumovirus comprises a nucleotide sequence encoding a zinc binding domain in the M2-1 protein wherein C21 is mutated, for example, to serine (C21S). In other embodiments, the nucleotide sequence encodes a zinc binding domain in the M2-1 wherein H25 is mutated, for example, to leucine (H25L). Where the M2-1 protien comprises two or more mutated amino acids, at least one of the mutations is a mutation of either C21 or of H25. Additional mutations can include mutations to one or both of C7 and C15. In particular embodiments, cysteine residues are mutated to serine residues and histidine is mutated to leucine, however, as described above any non-lethal mutation can be used.
Where the pneumovirus is an avian metapneumovirus, the pneumovirus comprises a nucleotide sequence encoding a zinc binding domain in the M2-1 protein wherein C21 is mutated, for example, to serine (C21S). In other embodiments, the nucleotide sequence encodes a zinc binding domain in the M2-1 wherein H25 is mutated, for example, to leucine (H25L). Where the M2-1 protein comprises two or more mutated amino acids, at least one of the mutations is a mutation of either C21 or of H25. Additional mutations can include mutations to one or both of C7 and C15. In particular embodiments, cysteine residues are mutated to serine residues and histidine is mutated to leucine, however, as described above any non-lethal mutation can be used.
In embodiments where the pneumovirus is a human respiratory syncytial virus, the pneumovirus comprises a nucleotide sequence encoding a zinc binding domain in the M2-1 protein wherein C21 is mutated, for example, to serine (C21S). In other embodiments, the nucleotide sequence encodes a zinc binding domain in the M2-1 wherein H25 is mutated, for example, to leucine (H25L). Where the M2-1 protein comprises two or more mutated amino acids, at least one of the mutations is a mutation of either C21 or of H25. Additional mutations can include mutations to one or both of C7 and C15. In particular embodiments, cysteine residues are mutated to serine residues and histidine is mutated to leucine, however, any non-lethal mutation can be used.
Any mutated pneumovirus described herein can be used in a vaccine to induce a protective immune response in a subject. A mutated pneumovirus is generally used in a vaccine for a wild-type pneumovirus of the same type. For example, a mutated human metapneumovirus can be used as a vaccine in a human subject, where administration of the vaccine comprising the mutated human metapneumovirus to the subject induces a protective immune response in the subject, thereby protecting the subject from future viral challenge by wild-type human metapneumovirus. In certain embodiments, the vaccine is a live attenuated vaccine.
Subjects that may benefit from a vaccine described herein include but are not limited to humans, fowl, including but not limited to turkey (Meleagris spp.), chicken (Gallus spp.), pheasant (Phasianus spp.), Muscovy duck (Cairina moschata), and guinea fowl (family Numididae), cattle, and rodents.
Vaccines of the present invention comprise a mutated pneumovirus described herein, or an immunogenic part thereof, in an amount or concentration sufficient to induce a protective immune response in a subject (i.e, an immunologically effective dose). It will be known to one of skill in the art how to determine the amount or concentration required to induce a protective immune response in the subject. In certain embodiments, an immunologically effective dose can be from about 103 PFU/kg to about 107 PFU/kg, or any range or value therein. In other embodiments the effective dose can be from about 105 PFU/kg to about 106 PFU/kg.
In certain embodiments, the mutated pneumovirus or an immunogenic part thereof is present in a vaccine composition. The vaccine composition, in addition to the mutated pneumovirus or immunogenic part thereof, may further comprise a pharmaceutically acceptable carrier, vehicle, excipient, or combination thereof, thereby forming a pharmaceutical vaccine composition. A vaccine composition can also comprise one or more adjuvants. Pharmaceutically acceptable carriers, vehicles, excipients, and adjuvants are generally well understood in the art, as is preparation of vaccines, including live attenuated vaccines.
Vaccine compositions can be administered to a subject by any known means for vaccine administration. Administration routes can include, but are not limited to intranasal administration, subcutaneous administration, intramuscular administration, intradermal administration, and oral administration. One of skill in the art will readily be able to identify an appropriate means for administering a vaccine, live attenuated vaccine, or vaccine composition described herein to a particular subject.
In certain embodiments, subjects are administered two or more doses of a vaccine described herein. A second dose of a vaccine can be administered at any interval following the initial dose. Following initial immunization, where a protective immune response is induced in a subject, additional doses of the same vaccine can prevent decreases in circulating neutralizing antibody levels, thereby prolonging the protective immune response. In certain embodiments, the interval between first and second immunizations is approximately one week, approximately, two weeks, approximately three weeks, approximately four weeks, approximately five weeks, approximately six weeks, approximately seven weeks, and approximately eight weeks. Alternatively, levels of neutralizing antibody in a subject can be monitored, with additional immunizations administered when neutralizing antibodies reach levels that no longer confer a protective effect.
Kits
Another embodiment described herein relates to kits for use with the compositions and methods described herein. Certain embodiments include kits having one or more pharmaceutical or vaccine compositions or boost compositions of use to prevent or treat subjects exposed to or having a pathogen or condition. The pathogen can include, but is not limited to, human metapneumovirus subtype A, human metapneumovirus subtype B, avian metapneumovirus subtype A, avian metapneumovirus subtype B, avian metapneumovirus subtype C, avian metapneumovirus subtype D, human respiratory syncytial virus type A, human respiratory syncytial virus type B, bovine respiratory syncytial virus, and pneumonia virus of mice. Kits can be portable, for example, able to be transported and used in remote areas. Other kits may be of use in a health care facility to immunize a subject, or to treat a subject having been exposed to a virus or suspected of having been exposed or at risk of exposure to a virus. Kits can include one or more pharmaceutical compositions and/or vaccine compositions comprising at least one mutant pneumovirus described herein that can be administered before, during, or after exposure to a virus. In other embodiments, kits include dehydrated formulations of pharmaceutical compositions and/or vaccine compositions described herein in order to prolong the half-life of the mutated pneumovirus contained therein (e.g., for stockpiling the pharmaceutical compositions and/or vaccine compositions in the event of an outbreak or providing treatments to remote areas).
Yet other embodiments, kits include a suitable container, such as vials, tubes, mini- or microfuge tubes, test tube, flask, bottle, syringe, or other suitable container. Where an additional component or agent is provided, the kit can contain one or more additional containers into which this agent or component can be placed. Kits herein will also typically include a means for containing the agent, composition and any other reagent containers in close confinement for commercial sale. Such containers can include plastic containers into which the desired vials are placed to be retained. Optionally, one or more additional agents such as other anti-viral agents, anti-fungal or anti-bacterial agents may be needed for compositions described, for example, for pharmaceutical compositions for use as a vaccine.
Definitions
The term “recombinant” is generally used to indicate that the material (e.g., a virus, a nucleic acid, a genetic construct or a protein) has been altered by technical means (i.e., non-naturally) through human intervention. As used herein, the term denotes material (e.g., a virus, a nucleic acid, a genetic construct or a protein) that has been altered by technical means of mutagenesis.
As used herein, the term “antibody” is intended to refer broadly to any immunologic binding agent such as IgG, IgM, IgA, IgD and IgE. “Antibody” may refer to any antibody-like molecule that has an antigen binding region, and includes antibody fragments such as Fab′, Fab, F(ab′)2, single domain antibodies (DABs), Fv, scFv (single chain Fv), and the like. A “neutralizing antibody” is intended to refer broadly to any antibody which defends a cell from an infectious body, such as a virus, by inhibiting or neutralizing the infectious body's biological activity.
As used herein, “immunogenicity” refers to the ability of a particular substance, such as an attenuated virus, to provoke or initiate an immune response in a subject. Immunogenicity refers to the ability of the substance to induce a humoral and/or cell mediated immune response.
“Composition” refers to any mixture, aqueous or non-aqueous solution, suspension, emulsion, gel, or the like, that contains two or more components. The components can be, for example, an immunostimulatory component that provides an immunostimulatory effect. The immunostimulatory components can be any combination of one or more live attenuated viruses, immunogenic part or parts thereof, one or more adjuvants, or any combination thereof. For example, a composition can comprise two adjuvants so that the composition forms an adjuvant combination. Alternatively, a composition can include an adjuvant and a live attenuated pneumovirus so that the mixture forms, e.g., a vaccine.
As used herein, the term “pharmaceutical composition” encompasses a product comprising one or more active ingredients, and an optional carrier, vehicle, or excipient comprising inert ingredients, as well as any product that results, directly or indirectly, from combination, complexation or aggregation of any two or more of the ingredients, or from dissociation of one or more of the ingredients, or from other types of reactions or interactions of one or more of the ingredients. Generally, pharmaceutical compositions are prepared by uniformly and intimately bringing the active ingredient into association with a liquid carrier, vehicle, or excipient, or a finely divided solid carrier, vehicle, or excipient, or both, and then, if necessary, shaping the product into the desired formulation. The pharmaceutical composition includes enough of the active ingredient to produce the desired effect. Accordingly, the pharmaceutical compositions described herein encompass any composition made by admixing a compound or recombinant pneumovirus mutant of the present invention and a pharmaceutically acceptable carrier, vehicle, or excipient. By “pharmaceutically acceptable” it is meant the carrier, vehicle, or excipient must be compatible with the other ingredients of the formulation and not deleterious to the recipient thereof.
“Vaccine” and “Vaccine Composition” as used herein, refers to a pharmaceutical composition that includes a live attenuated pneumovirus, or an immunogenic part or parts thereof. A vaccine can include components in addition to the live attenuated pneumovirus or immunogenic part or parts thereof, such as, for example, one or more adjuvants, a carrier, etc.
Numbering of amino acids, unless otherwise specified, is of amino acids comprising the pneumovirus M2-1 protein. The first amino acid (from the N-terminus) of M2-1 protein is designated amino acid 1. For example, C21 indicates the presence of cysteine at amino acid position 21 of a pneumovirus M2-1 protein, and the notation C21S indicates the substitution of cysteine at position 21 by serine.
“Immunologically effective dose” as used herein refers to any amount of a vaccine composition that will cause a reduction in one or more of the manifestations of viral challenge, such as viral lesions, viral load, rate of virus production, and mortality as compared to untreated control subjects. It is understood that the immunologically effective dose will be dependent on many factors, including but not limited to the age, sex, health, and weight of the subject. The ranges of immunologically effective doses described herein are not intended to be limiting, and represent possible dose ranges. In certain embodiments, dosage will be tailored to the individual subject. Tailored dosages used during vaccination can also vary depending on the particular nature of the live attenuated vaccine and viral vector used. Determination of dosing requirements is well within the ordinary skill of a physician.
The methods and embodiments described herein are further defined in the following Examples. Certain embodiments of the present invention are defined in the Examples herein. It should be understood that these Examples, while indicating certain embodiments of the invention, are given by way of illustration only. From the discussion herein and these Examples, one skilled in the art can ascertain the essential characteristics of this invention and without departing from the spirit and scope thereof, can make various changes and modifications of the invention to adapt it to various usages and conditions.
Cell lines. LLC-MK2 (ATCC CCL-7) cells were maintained in Opti-MEM medium (Life Technologies, Bethesda, Md.) supplemented with 2% fetal bovine serum (FBS). Vero E6 cells (ATCC CRL-1586) and BHK-SR19-T7 cells (provided by Apath, LLC, Brooklyn, N.Y.) were grown in Dulbecco's modified Eagle's medium (DMEM; Life Technologies) supplemented with 10% FBS. The medium for the BHK-SR19-T7 cells was supplemented with 10 μg/ml puromycin (Life Technologies) during every other passage to select for T7 polymerase-expressing cells.
Plasmids and site-directed mutagenesis. Plasmids encoding the hMPV minigenome, the full-length genomic cDNA of hMPV strain NL/1/00, and support plasmids expressing hMPV N protein (pCITE-N), P protein (pCITE-P), L protein (pCITE-L), and M2-1 protein (pCITE-M2-1) were provided by Ron A. M. Fouchier at the Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands. The F cleavage site in the genome of hMPV NL/1/00 was modified to a trypsin-independent F cleavage site. The M2-1 mutants of hMPV were generated by site-directed mutagenesis using the QuikChange methodology (Stratagene, La Jolla, Calif.). All constructs were sequenced at The Ohio State University Plant Microbe Genetics Facility to confirm the presence of the introduced mutations.
Expression and purification of recombinant hMPV M2-1 protein from E.coli. The hMPV M2-1 gene was PCR amplified from a cDNA clone of strain NL/1/00 and was inserted into E. coli expression system pGEX-4T-1 vector at BamHI and NotI sites. To facilitate the protein purification, the GST protein was fused to the N-terminus of M2-1 gene. The resulting plasmids were transformed into E. coli Rosetta (DE3) and grown at 37° C. until the absorbance at 600 nm reached 0.6-0.8. Cells were then chilled on ice for 10 min and protein expression was induced by addition of 20 μM of Isopropyl β-D-1-thiogalactopyranoside (IPTG) and 75 μM of ZnSO4. The cells were grown for an additional 20 h at 25° C. and harvested by centrifugation at 5,000 g for 10 mM. Bacterial pellets were resuspended in lysis buffer (40 mM Tris-HCl (pH 7.4), 1.0 M NaCl, 0.5 mM DTT, and 20 μM ZnSO4) supplemented with 1 mg/ml of lysozyme and protease inhibitor cocktail (Roche, Mannheim, Germany). After 30 min incubation on ice, the cells were lysed using sonication, followed by centrifugation at 15,000 g at 4° C. for 40 min. The supernatant was collected and loaded into a column containing 5 ml of Glutathione HiCap Matrix (Qiagen). The G resins were washed with 150 ml of resuspension buffer, followed by washing with 100 ml of the cleavage buffer (50 mM Tris-HCl (pH 7.6), 2.5 mM CaCl2, 150 mM NaCl, and 0.2 mM DTT). To isolate GST-free M2-1, 20 ml of the cleavage buffer containing 2 units/ml of thrombin (Sigma, St. Louis, Mo.) was loaded into the column and was incubated at room temperature for 8 h. Cleavage was halted by adding APMSF (Sigma).
Similarly, the GST fusion hMPV M2-1 mutants C7S, C15S, C21S and H25L were generated. The purified hMPV M2-1 proteins were dialyzed against PBS buffer containing 300 mM NaCl and 10 μM ZnSO4. Protein concentration was determined by the Bradford assay (Sigma).
Circular Dichroism (CD) spectroscopy. Recombinant hMPV M2-1 protein was dissolved in buffer (40 mM Tris-HCl pH7.4, 10 μM ZnCl2) and dialyzed against this same buffer at 4° C. overnight. 2 μM of M2-1 protein was incubated with increasing concentration of ethylenediaminetetraacetic acid (EDTA), and analyzed by an AVIV far-UV spectropolarimeter at 20° C. using a quartz microcell of 0.1 cm path length with a wavelength range of 190-260 nm at scanning speed of 50 nm per min. Ten individual readings were recorded and averaged. After subtraction of the baseline, the spectra were mathematically transformed into molar ellipticity.
Colorimetric determination of the zinc content. Purified hMPV M2-1 was dialyzed against 50 mM PBS (pH7.0) containing 0.3 M NaCl at 4° C. overnight. M2-1 proteins at concentrations of 2 μM, 3 μM, and 4 μM in 1 ml of solution containing 100 μM 4-(2-Pyridylazo) resorcinol (PAR) were incubated at 25° C. for 20 min and the absorbance at 500 nm was monitored for 5 min. Upon addition of 100 μM p-chloromercuriphenylsulfonic acid (PMPS), the release of strongly bound Zn2+ was monitored. The amount of Zn2+ bound to hMPV M2-1 was quantified by comparing the sample readings to a standard curve generated using 2 μM, 3 μM, and 4 μM ZnSO4 (Sigma). Buffer containing 50 mM PBS (pH7.0), 0.3M NaCl, and 100 μM PAR was used as the blank control. The Zn2+ content of GST-M2-1 and mutants was measured at 2 μM and compared with rM2-1 at the same Zn2+ concentration.
Glutaraldehyde cross-linking. 1.2 μg of rM2-1, GST-M2-1 or mutant proteins were diluted in 20 id of reaction buffer (50 mM PBS pH7.0, 300 mM NaCl) and were incubated with increasing amounts of glutaraldehyde from 0-2% at 25° C. for 30 sec. The reaction was stopped by the addition of Tris-HCl (pH7.4) at a final concentration of 50 mM. Subsequently, the cross-linked products were analyzed by 12% SDS-PAGE and the proteins were visualized by Coomassie blue staining.
Recovery of rhMPVs from the full-length cDNA clones. RhMPVs were rescued using a reverse genetics system. BHK-SR19-T7 cells (provided by Apath LLC), which stably express T7 RNA polymerase, were transfected with 5.0 μg of plasmid phMPV carrying the full-length hMPV genome, 2.0 μg of pCITE-N, 2.0 μg of pCITE-P, 1.0 μg of pCITE-L, and 1.0 μg of pCITE-M2-1 using Lipofectamine 2000 (Life Technologies). At day 6 posttransfection, the cells were harvested using scrapers and were co-cultured with LLC-MK2 cells at 50-60% confluence. When extensive cytopathic effects (CPE) were observed, the cells were subjected to three freeze-thaw cycles, followed by centrifugation at 3,000×g for 10 min. The supernatant was subsequently used to infect new LLC-MK2 cells. The successful recovery of the rhMPVs was confirmed by immunostaining, agarose overlay plaque assay, and reverse transcription (RT)-PCR.
Immunostaining plaque assay. Vero E6 cells were seeded in 24-well plates and infected with serial dilutions of rhMPV. At day 6 post-infection, the supernatant was removed and cells were fixed in a pre-chilled acetone-methanol solution at room temperature for 15 min. Cells were permeabilized in phosphate-buffered saline (PBS) containing 0.4% Triton X-100 at room temperature for 10 min and blocked at 37° C. for 1 h using 1% bovine serum albumin (BSA) in PBS. The cells were then labeled with an anti-hMPV N-protein primary monoclonal antibody (Millipore, Billerica, Mass.) at a dilution of 1:1,000, followed by incubation with horseradish peroxidase (HRP)-labeled rabbit antimouse secondary antibody (Thermo Scientific, Waltham, Mass.) at a dilution of 1:5,000. After incubation with 3-amino-9-ethylcarbazole (AEC) chromogen substrate (Sigma, St. Louis, Mo.), positive cells were visualized under a microscope. The viral titer was calculated as the number of PFU per ml.
Viral replication kinetics in LLC-MK2 cells. Confluent LLC-MK2 cells in 35-mm dishes were infected with wild-type (wt) rhMPV or mutant rhMPV at an MOI of 0.01. After 1 h of adsorption, the inoculum was removed and the cells were washed three times with PBS. Fresh DMEM (supplemented with 2% FBS) was added and the infected cells were incubated at 37° C. At different time points postinfection, the supernatant and cells were harvested by three freeze-thaw cycles, followed by centrifugation at 1,500×g at room temperature for 15 min. The virus titer was determined by an immunostaining assay in Vero E6 cells.
Replication and pathogenesis of rhMPV in cotton rats. Twenty five 4-week-old female specific-pathogen-free (SPF) cotton rats (Harlan Laboratories, Indianapolis, Ind.) were randomly divided into five groups (5 cotton rats per group). Prior to virus inoculation, the cotton rats were anesthetized with isoflurane. The cotton rats in group 1 were inoculated with 2.0×105 PFU of wt rhMPV and served as positive controls. The cotton rats in groups 2-5 were inoculated with 2.0×105 PFU of four rhMPV mutants (rhMPV-C7S, C15S, C21S, and H25L). The cotton rats in group 6 were mock infected with 0.1 ml of Opti-MEM medium and served as uninfected controls. Each cotton rat was inoculated intranasally with a volume of 100 μl. After inoculation, the animals were evaluated on a daily basis for mortality and the presence of any respiratory symptoms. At day 4 postinfection, the cotton rats were sacrificed, and lungs and nasal turbinates were collected for both virus isolation and histological analysis.
Immunogenicity of rhMPVs in cotton rats. Twenty five cotton rats (Harlan Laboratories, Indianapolis, Ind.) were randomly divided into five groups (5 cotton rats per group). The cotton rats in group 1 were mock infected with Opti-MEM medium and used as an infected control, and those in groups 2 to 4 were intranasally inoculated with 2.0×105 PFU of wt rhMPV, rhMPV-C21S, or H25L in 0.1 ml Opti-MEM medium. The cotton rats in group 5 were inoculated with DMEM and served as the unimmunized challenged control. After immunization, the cotton rats were evaluated daily for mortality and the presence of any symptoms of hMPV infection. Blood samples were collected from each rat weekly by facial vein retro-orbital bleeding, and serum was isolated for neutralizing antibody detection. At week 4 post-immunization, the cotton rats in groups 2 to 5 were challenged intranasally with wild-type rhMPV at a dose of 1.0×106 PFU per cotton rat.
After challenge, the animals were evaluated twice every day for mortality and the presence of any symptoms of hMPV infection. At day 4 post-challenge, all cotton rats from each group were euthanized by CO2 asphyxiation. The lungs and nasal turbinates from each cotton rat were collected for virus isolation and histological evaluation. The immunogenicity of rhMPV mutants was evaluated using the following methods: (i) humoral immunity was determined by analysis of serum neutralization of virus using an endpoint dilution plaque reduction assay; (ii) viral titers in the nasal turbinates and lungs were determined by an immunostaining plaque assay; and, (iii) pulmonary histopathology and viral antigen distribution were determined using the procedure described below. The protection was evaluated with respect to viral replication, antigen distribution, and pulmonary histopathology
Pulmonary histology. After sacrifice, the right lung of each animal was removed, inflated, and fixed with 4% neutral buffered formaldehyde. Fixed tissues were embedded in paraffin and sectioned at 5 μm. Slides were then stained with hematoxylin-eosin (H&E) for the examination of histological changes by light microscopy. The pulmonary histopathological changes were reviewed by 2-3 independent pathologists. Histopathological changes were scored to include the extent of inflammation (focal or diffuse), the pattern of inflammation (peribronchiolar, perivascular, interstitial, alveolar), and the nature of the cells making up the infiltrate (neutrophils, eosinophils, lymphocytes, macrophages).
Immunohistochemical (IHC) staining. The right lung of each animal was fixed in 10% neutral buffered formaldehyde and embedded in paraffin. Five-micrometer sections were cut and placed onto positively charged slides. After deparaffinization, sections were incubated with target retrieval solution (Dako, Carpinteria, Calif.) for antigen retrieval. After antibody block, a primary mouse anti-hMPV monoclonal antibody (Virostat, Portland, Me.) was added for 30 min at room temperature, followed by incubation with a biotinylated horse anti-mouse secondary antibody (Vector Laboratories, Burlingame, Calif.). Slides were further incubated with ABC Elite complex to probe biotin (Vector Laboratories) and then developed using a 3,3′-diaminobenzidine (DAB) chromogen kit (Dako) and hematoxylin as a counterstain. Lung sections from hMPV-infected and uninfected samples were used as positive and negative controls, respectively.
Determination of hMPV-neutralizing antibody. hMPV-specific neutralizing antibody titers were determined using a plaque reduction neutralization assay. Briefly, cotton rat sera were collected by retro-orbital bleeding weekly until challenge. The serum samples were heat inactivated at 56° C. for 30 min. Two-fold dilutions of the serum samples were mixed with an equal volume of DMEM containing approximately 100 PFU/well hMPV NL/1/00 in a 96-well plate, and the plate was incubated at room temperature for 1 h with constant rotation. The mixtures were then transferred to confluent Vero E6 cells in a 96-well plate in triplicate. After 1 h of incubation at 37° C., the virus-serum mixtures were removed and the cells were overlaid with 0.75% methylcellulose in DMEM and incubated for another 4 days before virus plaque titration. The plaques were counted and 50% plaque reduction titers were calculated as the hMPV-specific neutralizing antibody titers.
Determination of viral titer in lung and nasal turbinate. The nasal turbinate and the left lung from each cotton rat were removed, weighed, and homogenized in 1 ml of PBS solution using a Precellys 24 tissue homogenizer (Bertin, Md.) following the manufacturer's recommendations. The presence of infectious virus was determined by an immunostaining plaque assay in Vero cells, as described above.
Statistical analysis. Quantitative analysis was performed by either densitometric scanning of autoradiographs or by using a phosphorimager (Typhoon; GE Healthcare, Piscataway, N.J.) and ImageQuant TL software (GE Healthcare, Piscataway, N.J.). Statistical analysis was performed by one-way multiple comparisons using SPSS (version 8.0) statistical analysis software (SPSS Inc., Chicago, Ill.). A P value of <0.05 was considered statistically significant.
The amount of zinc bound to the hMPV M2-1 protein was characterized. A time-course colorimetric assay was developed that could directly and quantitatively measure Zn2+ binding. Various amounts of highly purified M2-1 protein were incubated with 100 μM 4-(2-Pyridylazo) resorcinol (PAR), the metallochromic indicator, and the release of Zn2+ bound to the M2-1 protein was monitored upon addition of 100 μM PMPS. Subsequently, the amount of Zn2+ released was determined using standard curves generated by ZnSO4. At concentrations of 2, 3, and 4 μM Zn2+, the absorbance at 500 nm was 0.14, 0.20, and 0.27, respectively (
Sequence alignment found that M2-1 proteins of all known pneumoviruses possess a putative zinc binding motif (CCCH) (
It was then determined whether the zinc binding motif is essential for zinc binding activity. Cysteine residues at positions 7, 15 and 21 were changed to serine individually and the histidine at position 25 was changed to leucine. To enhance the solubility of mutant M2-1 proteins, a GST tag was fused to the N terminus of M2-1. All mutant M2-1 proteins were expressed and purified (
Having demonstrated that the M2-1 protein coordinates zinc ions, it was then determined whether the secondary structure of M2-1 is altered by EDTA, which can chelate metal ions, including zinc. As shown in
The effect of mutations in the zinc binding motif on oligomerization of M2-1 was determined by a chemical cross-linking assay. Briefly, 1.0 μg of rM2-1 protein (without any tag) was cross-linked by an increasing concentration of glutaraldehyde (from 0 to 0.2%) and the resulting products were resolved by SDS-PAGE. As shown in
Subsequently, the effects of mutations in zinc binding motif on oligomerization of M2-1 were examined. The predicted M2-1 tetramer (196 kDa protein) was not observed for C7S, C15S, C21S and H25L (
Prior to the studies described herein, it was not known whether this zinc binding motif is, indeed, required for zinc coordination. The results described herein show that the third cysteine and the histidine in the zinc binding motif (CCCH) of hMPV M2-1 are crucial for zinc coordination, while the first two cysteine residues each play a minor role in zinc binding. All four M2-1 mutants affected the formation of M2-1 tetramers, suggesting that zinc binding residues are essential for oligomerization of M2-1. The recently-solved structure of hMPV M2-1 showed that the oligomerization domain maps to amino acids 32-58 and that this region folds as an α-helix, which is exposed on the protein surface (
aData were collected using hMPV M2-1 structure (PDB ID: 4CS7). C15 is also involved in interprotein interaction between chains in the tetramer. There are four chains (A, B, C, and E) in the tetramer. C15 (chain E) interacts with S75 (chain A); and C15 (chain B) interacts with T76 (chain E).
To determine the role of the zinc binding activity in viral replication and pathogenesis, the zinc binding motif of M2-1 in an infectious cDNA clone of hMPV lineage A strain NL/1/00 was mutated. All four recombinant viruses (rhMPV-C7S, C15S, C21S, and H25L) were successfully recovered. The viruses were passed three times in LLC-MK2 cells and stained by a monoclonal antibody against N protein. As shown
The ability of these recombinant viruses to form plaques in LLC-MK2 cells was determined by an agarose overlay plaque assay (
Subsequently, all hMPV mutants were plaque purified. The M2-1 gene of each recombinant virus was amplified by RT-PCR, sequenced, and the presence of the desired mutation was confirmed. Finally, the entire genome of each hMPV mutant was amplified and sequenced. The results showed that no additional mutations were found in the genome except for the introduced mutation in M2-1 gene. These hMPV mutants were passed 10 times in LLC-MK2 cells and sequencing found that all of mutants retained the desired mutation, demonstrating that these zinc binding-defective hMPV mutants are genetically stable in cell culture.
The replication kinetics of recombinant hMPV carrying mutations in zinc binding motif were determined in LLC-MK2 cells (
The replication and pathogenesis of rhMPV carrying mutations in the zinc binding motif were determined in cotton rats, the best small animal model available for hMPV. Four-week-old SPF cotton rats were inoculated intranasally with 1.0×106 PFU of wt rhMPV or rhMPV mutants. At day 4 post-inoculation, cotton rats were terminated and viral replication in nasal turbinate and lungs and pulmonary histology were evaluated (Table 2). rhMPV replicated efficiently in the nasal turbinates and lungs of all five cotton rats, with average viral titers of 105.68 and 104.25 PFU/g tissue, respectively. Recombinant rhMPV-C7S and C15S, which retained 60% of zinc binding activity, replicated as efficiently as rhMPV in cotton rats, producing similar titers in both the nasal turbinates and the lungs of all five cotton rats (P>0.05). Recombinant rhMPV-C21S and H25L, which abolished zinc binding activity, were highly attenuated in replication in cotton rats. No infectious virus was detected in either nasal turbinate or lungs in rhMPV-C21S infected cotton rats. Only one out of five cotton rats had a low level of infectious virus (102.3 PFU/ml) in nasal turbinate and no infectious virus was found in lungs.
Pulmonary histology showed that rhMPV caused moderate histological lesions, including interstitial pneumonia, peribronchial lymphoplasmacytic infiltrates, mononuclear cell infiltrate, and edematous thickening of the bronchial submucosa (
Taken together, these results showed that rhMPV-C7S and C15S replicated efficiently in cotton rats and caused similar histologic lesions as rhMPV, whereas rhMPV-C21S and H25L were highly attenuated in replication in both the upper and lower respiratory tracts of cotton rats.
Along with the data discussed in Example V, these results show that the zinc binding activity of hMPV M2-1 is dispensable for viral replication in vitro, but essential for viral replication and pathogenesis in vivo. Zinc binding-deficient rhMPVs were highly attenuated in replication in vitro cell culture and in vivo cotton rats and did not cause lung histologic lesion. In contrast, rhMPVs retaining 60% of zinc binding activity were capable of replicating as efficiently as wild-type hMPV in vitro and in vivo, and caused significant lung damage.
A Cotton rats were inoculated intranasally with DMEM or 2 × 105 pfu wild-type rhMPV or rhMPV mutants. At day 4 post immunization, animals were euthanized for pathology study.
B For rhMPV-H25L, 1 out of 5 cotton rats had detectable virus with a titer of 2.3 log PFU/g. “ND” indicates that infectious virus was not detectable. Value within a column followed by the different lowercase letters (a and b) are significantly different (P < 0.05).
C The severity of lung histology was scored for each lung tissue. Average score for each group was shown. 0 = no change; 1 = mild change; 2 = moderate change; and 3 = severe change.
D The amount of hMPV antigen expression in lung was scored. Average score for each group was shown. 0 = no antigen; 1 = small amount; 2 = moderate amount; and 3 = large amount.
The immunogenicity of the two attenuated mutants (rhMPV-C21S and H25L) was determined by vaccination of cotton rats, followed by challenge with rhMPV. After vaccination, serum antibody levels were determined weekly by a plaque reduction neutralization assay. As shown in
At week 4 post-immunization, all vaccinated cotton rats were challenged with wt rhMPV and cotton rats were terminated at day 4 post-inoculation. No infectious virus was detectable in either nasal turbinates or lungs from the animals vaccinated with either rhMPV-C21S or H25L, followed by rhMPV challenge (Table 3). Pulmonary histology showed that the unvaccinated challenged control had moderate pathological changes characterized by interstitial pneumonia, mononuclear cell infiltrate, and edematous thickening of the bronchial submucosa. In contrast, no or only mild histological changes were found in the lungs of cotton rats vaccinated with rhMPV-C21S and rhMPV-H25L (Table 3). No enhanced lung damage was found for the mutants. IHC showed that large numbers of viral antigens were found at the luminal surface of the bronchial epithelial cells in lung tissues from unvaccinated challenged controls (
A Animals were immunized intranasally with DMEM or 2 × 105 pfu wild-type rhMPV or rhMPV mutants. At day 28 post immunization, animals were challenged with 1 × 106 pfu wild-type rhMPV.
B ND indicates infectious virus was not detectable.
C The severity of lung histology was scored for each lung tissues. Average score for each group was shown. 0 = no change; 1 = mild change; 2 = moderate change; and 3 = severe change. Value within a column followed by the different lowercase letters (a and b) are significantly different (P < 0.05).
Collectively, these results demonstrate that rhMPVs lacking zinc binding activity are not only sufficiently attenuated, but also capable of triggering high levels of antibody and providing protection against viral challenge by hMPV.
These zinc binding-deficient rhMPVs are ideal live attenuated vaccines. These two rhMPV mutants grow to high titers in cell culture, making it economically feasible for vaccine production. No revertants or additional mutations were detected after ten passages of these mutants in cell culture, showing that they are genetically stable. Importantly, these two rhMPV mutants were sufficiently attenuated, yet retained high immunogenicity.
Previously, it was shown that rhMPV lacking the entire M2-1 gene (rhMPVΔM2-1) was overly attenuated so that it failed to trigger either an hMPV-specific antibody response or protective immunity in animals (Buchholz U J et al., 2005. Deletion of M2 gene open reading frames 1 and 2 of human metapneumovirus: effects on RNA synthesis, attenuation, and immunogenicity. J Virol 79:6588-97). In contrast to rhMPVΔM2-1, rhMPVs lacking zinc binding activity described herein were defective in replication in the upper and lower respiratory tracts of cotton rats, but were capable of inducing high levels of neutralizing antibody and providing protection against subsequent challenge by rhMPV.
Cell lines. HEp-2 and Hela cells were maintained in Opti-MEM medium (Life Technologies, Bethesda, Md.) supplemented with 10% fetal bovine serum (FBS).
Plasmids and site-directed mutagenesis. Plasmids encoding the hRSV minigenome (MP169) expressing the green fluorescent protein (GFP) and the full-length genomic cDNA of hRSV strain A2 (RW30) are modified versions of plasmids provided by Peter Collins (National Institutes of Health). Peter Collins also provided the support plasmids expressing RSV N protein (pN), P protein (pP), L protein (pL), and M2-1 protein (pM2-1). MVA-T7, a vaccinia virus derivative expressing the T7 polymerase was provided by Bernard Moss. The M2-1 mutants of RSV were generated by site-directed mutagenesis using QuikChange (Stratagene, La Jolla, Calif.). All constructs were sequenced to confirm the presence of the introduced mutations.
Expression and purification of recombinant RSV M2-1 protein from E.coli. The RSV M2-1 gene was PCR amplified from the RW30 cDNA clone of strain A2 and was inserted into the E. coli expression plasmid pGEX-4T-1 at the BamHI and NotI sites. To facilitate protein purification, the GST sequence was fused to the N-terminus of the M2-1 gene. The resulting plasmids were transformed into E. coli Rosetta (DE3) and grown at 37° C. until the absorbance at 600 nm reached 0.6-0.8. Cells were chilled on ice for 10 min and protein expression was induced by the addition of 20 μM Isopropyl β-D-1-thiogalactopyranoside (IPTG) and 75 μM ZnSO4. The cells were grown for an additional 20 h at 25° C. and harvested by centrifugation at 5,000 g for 10 mM. Bacterial pellets were resuspended in lysis buffer [40 mM Tris-HCl (pH 7.4), 1.0 M NaCl, 0.5 mM DTT, and 20 μM ZnSO4] supplemented with 1 mg/ml of lysozyme and protease inhibitor cocktail (Roche, Mannheim, Germany). After 30 min incubation on ice, the cells were lysed using sonication, followed by centrifugation at 15,000 g at 4° C. for 40 min. The supernatant was collected and loaded into a column containing 5 ml of Glutathione HiCap Matrix (Qiagen).
The G resin was washed with 150 ml of resuspension buffer, followed by washing with 100 ml of cleavage buffer (50 mM Tris-HCl (pH 7.6), 2.5 mM CaCl2, 150 mM NaCl, and 0.2 mM DTT). To isolate GST-free M2-1, 20 ml of the cleavage buffer containing 2 units/ml of thrombin (Sigma, St. Louis, Mo.) was loaded into the column and was incubated at room temperature for 8 h. Cleavage was halted by adding APMSF (Sigma). Similarly, the GST fusion RSV M2-1 mutants C7S, C15S, C21S and H25L were generated. The purified RSV M2-1 proteins were dialyzed against PBS buffer containing 300 mM NaCl and 10 μM ZnSO4. Protein concentration was determined by the Bradford assay (Sigma).
Colorimetric determination of the zinc content. Purified RSV M2-1 was dialyzed against 50 mM PBS (pH 7.0) containing 0.3 M NaCl at 4° C. overnight. RSV M2-1 proteins at concentrations of 2 μM, 3 μM, and 4 μM in a 1 ml of solution containing 100 μM 4-(2-Pyridylazo) resorcinol (PAR) were incubated at 25° C. for 20 min and the absorbance at 500 nm was monitored for 5 min. Upon addition of 100 μM p-chloromercuriphenylsulfonic acid (PMPS), the release of strongly bound Zn2+ was monitored. The amount of Zn2+ bound to RSV M2-1 was quantified by comparing the sample readings to a standard curve generated using 2 μM, 3 μM, and 4 μM ZnSO4 (Sigma). Buffer containing 50 mM PBS (pH7.0), 0.3 M NaCl, and 100 μM PAR was used as the blank control. The zinc ion content of GST-M2-1 and mutants were measured at 2 μM and compared with rM2-1 at the same zinc concentration.
Recovery of recombinant RSV-GFP (rgRSV) from the full-length cDNA clones. The full-length cDNA clones carrying wild-type M2-1 and M2-1 with mutations in its zinc binding motif were rescued as replicating rgRSV using the reverse genetics system. HEp-2 cells were infected with MVA-T7 at an MOI of 0.1. At 1 h post-infection, cells were transfected with 5.0 μg of plasmid RW30 carrying the full-length RSV genome, 2.0 μg of pN, 2.0 μg of pP, 1.0 μg of pL, and 1.0 μg of pM2-1 using Lipofectamine 2000 (Life Technologies). At day 4 posttransfection, the cells were harvested using scrapers and were cocultured with fresh HEp-2 cells at 50-60% confluence. When extensive cytopathic effects (CPE) were observed, the cells were subjected to three freeze-thaw cycles, followed by centrifugation at 3,000×g for 10 min. The supernatant was subsequently used to infect fresh HEp-2 cells. The successful recovery of the rgRSVs was confirmed by plaque assay and reverse transcription (RT)-PCR.
Viral replication kinetics in HEp-2 cells. Confluent HEp-2 cells in 35-mm dishes were infected with wild-type (wt) or mutant rgRSV at an MOI of 0.01. After 1 h of adsorption, the inoculum was removed and the cells were washed three times with PBS. Fresh DMEM (supplemented with 2% FBS) was added and the infected cells were incubated at 37° C. At different time points postinfection, the expression of GFP by each rgRSV was photographed.
To determine the zinc binding activity of RSV M2-1, the recombinant RSV M2-1 (rM2-1) protein was expressed in E. coli. To facilitate the purification of RSV M2-1, a GST tag was fused to its N terminus. As shown in
Unlike the hMPV M2-1 protein, the hRSV M2-1 protein is essential for virus replication. First, it was previously shown that deletion of the entire M2-1 or partial deletions in M2-1 were lethal to hRSV (Collins P L, Camargo E, and Hill M G, 1999. Support plasmids and support proteins required for recovery of recombinant respiratory syncytial virus. Virology 259:251-5; Collins P L et al., 1995. Production of infectious human respiratory syncytial virus from cloned cDNA confirms an essential role for the transcription elongation factor from the 5′ proximal open reading frame of the M2 mRNA in gene expression and provides a capability for vaccine development. PNAS USA 92:11563-67; Tang R S et al., 2001. Requirement of cysteines and length of the human respiratory syncytial virus M2-1 protein function and virus availability. J. Virol. 75:11328-35). This result is consistent with the processivity function of M2-1. Second, it was also shown that single amino acid substitutions (C to G) in the first three cysteine residues (namely C7G, C15G, and C21G) were lethal to RSV, preventing recovery of the recombinant virus. Until the present studies, the impact of mutations in H25 on the hRSV life cycle had not been studied.
As described and shown herein, a different mutagenesis strategy was followed. Namely, the first three cysteine residues of M2-1, at positions 7, 15 and 21, were changed to serine individually, and the histidine at position 25 was changed to leucine. Each of these mutations were inserted individually into an infectious cDNA clone of RSV with GFP inserted as the first gene, between the leader and N gene of RW30. All four recombinant viruses (rgRSV-C7S, C15S, C21S, and H25L) were recovered. As shown in
While the invention has been described with reference to various embodiments, it should be understood by those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof without departing from the essential scope of the invention. In addition, many modifications may be made to adapt a particular situation or material to the teachings of the invention without departing from the essential scope thereof.
Therefore, it is intended that the invention not be limited to the particular embodiments disclosed herein contemplated for carrying out this invention, but that the invention will include all embodiments falling within the scope of the claims.
This application is a U.S. National Phase application of International Patent Application No. PCT/US2016/08975 filed Feb. 22, 2016 which claims the benefit of United States Provisional Application No. 62/118,752, filed on Feb. 20, 2015, each of which is expressly incorporated herein by reference in its entirety for all purposes.
This invention was made with government support under Grant No. R01AI090060 awarded by National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health. The government has certain rights in the invention.
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/US2016/018975 | 2/22/2016 | WO | 00 |
| Publishing Document | Publishing Date | Country | Kind |
|---|---|---|---|
| WO2016/134378 | 8/25/2016 | WO | A |
| Number | Name | Date | Kind |
|---|---|---|---|
| 7205013 | Jin et al. | Apr 2007 | B2 |
| 20020119446 | Wertz et al. | Aug 2002 | A1 |
| 20040241188 | Collins | Dec 2004 | A1 |
| 20120308602 | Jin et al. | Dec 2012 | A1 |
| Entry |
|---|
| Tang et al. Requirement of Cysteines and Length of the Human Respiratory Syncytial Virus M2-1 Protein for Protein Function and Virus Viability. J. Virol. Dec. 2001, vol. 75, p. 11328-11335. |
| Tang et al. Requirement of Cysteines and Length of the Human Respiratory Syncytial Virus M2-1 Protein for Protein Function and Virus Viability. J. Virol. 2001, 75(23): 11328-11335. |
| Hardy et al. The Cys3-His1 Motif of the Respiratory Syncytial Virus M2-1 Protein Is Essential for Protein Function. J. Virol. 2000, 74(13): 5880-5885. |
| International Search Report and Written Opinion issued in PCT/US2016/018975, dated Apr. 29, 2016, 12 pages. |
| Jin et al. “Requirement of Cysteines and Length of the Human Respiratory Syncytial Virus N2-1 Protein for Protein Function and Virus Viablity,” Journal of Virology, Dec. 1, 2001, vol. 75, Issue 23, pp. 11328-11335. |
| Number | Date | Country | |
|---|---|---|---|
| 20180066238 A1 | Mar 2018 | US |
| Number | Date | Country | |
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| 62118752 | Feb 2015 | US |
