Latin name: Pinus taeda.
Variety denomination: ‘CF LP1-7696’.
A new variety of loblolly pine tree (Pinus taeda), has been discovered. This selection has been designated as ‘CF LP1-7696.’
This new variety is a progeny of two second generation selections. The female parent is a progeny of two first generation selections made in Cherokee County, Tex. and Tyler County, Tex. The male parent is a progeny of an open pollinated first generation selection made in Montgomery County, Tex.
Cross pollination occurred in early 2000 followed by induction and cryopreservation of embryogenic tissue in 2001. First somatic seedlings were produced in 2002 and planted in early 2003 in three field experiments. A total of 30 ramets were planted at 10 ramets per field experiment. The field experiments are located in Texas and Louisiana.
A new and distinct cultivar of loblolly pine (Pinus taeda) is distinctly characterized by high growth rate, good resistance to fusiform rust, excellent stem straightness, medium crown width, medium number of whorls, medium branch angle, medium branch diameter and which is mature for commercial harvesting sooner than conventionally grown trees under the ecological conditions prevailing in the Gulf Coastal Plains of the United States.
The Pinus taeda plants of this variety were asexually propagated using an advanced form of micropropagation called somatic embryogenesis carried out at a production facility in Victoria, Canada. Somatic embryogenesis uses a complex process which relies on the splitting of one embryo into many identical embryos. Somatic embryos can then be grown into plants which are all identical genetically. The asexual propagation occurs at an earlier stage in the plant's life cycle than most other micropropagated plants. The detailed methods for somatic embryogenesis used for asexually propagating conifers in general are described in U.S. Pat. No. 6,372,496 and for loblolly pine in particular in U.S. patent application Publication No. 2004/0203150.
The drawings are color photographs showing the new variety of loblolly pine.
The botanical details of this new and distinct variety of loblolly pine tree follow. All color descriptions are made in reference to The Royal Horticultural Society (R.H.S.) Colour Chart (2005).
Compared to unimproved loblolly pine trees, ‘CF LP1-7696’ is characterized by high growth rate, good resistance to fusiform rust (caused by Cronartium quercuum (Berk.) Miyabe ex Shirai f. sp. fusiforme (Cumm.) Burds. et Snow), excellent stem straightness, medium crown width, medium number of whorls, medium branch angle and medium branch diameter.
Although the new variety of loblolly pine tree possesses the detailed characteristics noted above as a result of the growing conditions prevailing in the test locations, it is to be understood that the variations of the usual magnitude and characteristics incident to changes in growing conditions, irrigation, fertilization, pruning, pest control, climatic variations and the like are to be expected. An example of ‘CF LP1-7696’ can be found at The Campbell Group Beulah year 2003 line trial, Angelina county, Tex.
Microsatellite markers (SSR's) were used to generate a unique DNA fingerprint for the variety. Young foliage samples from 6 ramets of LP1-7696 variety and from the parental trees used to make the LP1 cross were collected for DNA fingerprinting. The DNA extraction protocol of Doyle and Doyle (1987) was used after slight modifications. DNA fingerprinting of parents and the LP1-7696 variety was conducted using a set of nine microsatellite markers (Elsik et al., 2000; Auckland et al., 2002; Echt et al., 2008). Table 1 shows the sequences and conditions for each primer.
Microsatellite products were detected by M13 tailed primer (Oettling et al., 1995) or infrared dye(IRD)-labeled primer. The amplification products were electrophoresed on 5.5% Long Ranger polyacrylamide gels using a LiCor 4200 automated sequencer (LiCor Inc., Lincoln, Neb.).
The observed parental genotypes and their expected offspring's genotypes at nine studied SSR loci are presented in Table 2. LP1-7696 fingerprint based on nine loci is presented in Table 3.
Number | Name | Date | Kind |
---|---|---|---|
PP19447 | Pait et al. | Nov 2008 | P3 |
PP19793 | Pait et al. | Mar 2009 | P3 |
20070079408 | Pait et al. | Apr 2007 | P1 |
Number | Date | Country | |
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20100325765 P1 | Dec 2010 | US |