Patent Grant
PP19793
Latin name: Pinus taeda.
Variety denomination: ‘CF Q7766’.
A new variety of loblolly pine tree (Pinus taeda), has been discovered. This selection has been designated as ‘CF Q7766.’
This new variety is a progeny of a second generation selection pollinated by a first generation selection. Female parent is an open pollinated progeny of Georgetown County, S.C. first generation selection. Male parent is a first generation selection made in Barnwell County S.C.
Cross pollination occurred in early 1998 followed by induction and cryopreservation of embryogenic tissue in 1999. First somatic seedlings were produced in 2000 and planted in early 2001 in seven field experiments. A total of 47 ramets (independent members of a clone) were planted ranging from 4 to 8 ramets per field experiment. The field experiments are located in Mississippi, Florida, Georgia and South Carolina.
A new and distinct cultivar of loblolly pine (Pinus taeda) is distinctly characterized by great resistance to fusiform rust and pitch canker, exceptionally high growth rate, excellent stem straightness, distinctive very long internodes, narrow crown, and which is mature for commercial harvesting sooner than conventionally grown trees under the ecological conditions prevailing in the Piedmont, Atlantic and Gulf Coastal Plains, and Mid-Continent regions of the United States.
The Pinus taeda plants of this variety were asexually propagated using an advanced form of micropopagation called somatic embryogenesis carried out at CellFor's production facility in Victoria, Canada. Somatic embryogenesis uses a complex process which relies on the splitting of one embryo into many identical embryos. Somatic embryos can then be grown into plants which are all identical genetically. The asexual propagation occurs at an earlier stage in the plant's life cycle than most other micropropagated plants. The detailed methods for somatic embryogenesis used for asexually propagating conifers in general are described in U.S. Pat. No. 6,372,496 and for loblolly pine in particular in U.S. patent application 2004/0203150.
The drawings are color photographs showing the new variety of loblolly pine including the long internode length.
The botanical details of this new and distinct variety of loblolly pine tree follow. All color descriptions are made in reference to The Royal Horticultural Society (R.H.S.) Colour Chart (2005).
Compared to unimproved loblolly pine trees, ‘CF Q7766’ is characterized by exceptionally high growth rate, great resistance to fusiform rust (caused by Cronartium quercuum (Berk.) Miyabe ex Shirai f. sp. fusiforme (Cumm.) Burds. et Snow), great resistance to pitch canker (caused by Fusarium circinatum Nirenburg et O'Donnell), distinctive very long internodes, excellent stem straightness, narrow crown, small to medium branch diameter, flat to medium branch angle and low incidence of forking.
Although the new variety of loblolly pine tree possesses the detailed characteristics noted above as a result of the growing conditions prevailing in the seven test locations, it is to be understood that the variations of the usual magnitude and characteristics incident to changes in growing conditions, irrigation, fertilization, pruning, pest control, climatic variations and the like are to be expected. An example of ‘CF Q7766’ resulting from asexual reproduction can be found at Plum Creek Oliver year 2001 line trial, Screven county, Ga.
Microsatellite markers were used to generate a unique DNA fingerprint for the variety. Vegetative buds and/or foliar material from eight individuals each produced by controlled crossing among parents for DNA fingerprinting. The DNA extraction protocol of Doyle and Doyle (1987) was used after slight modifications. DNA fingerprinting of parents and their offspring was initially conducted using a set of nine microsatellite markers (Auckland et. al. 2002) and a final set of five primer pairs were selected for the two lines mentioned above (see Table 1, for sequences and conditions of SSR primers). Primer selection was based on their ability to produce unique alleles and the presence of a high level of polymorphism.
Microsatellite products were detected by M13 tailed primer (Oetting et al., 1995) or infra-red dye (IRD)-labeled primer. The amplification products were electrophoresed on 5.5% Long Ranger polyacrylamide gels using a LiCor 4200 automated sequencer (LiCor Inc., Lincoln, Nebr.). For each family, the female and male parents, as well as eight offspring were genotyped.
The observed parental genotypes and their expected offspring's genotypes at five studied SSR loci of each family are presented in Table 2.
In general, offspring genotypes segregated following expected simple Mendelian segregation (see Table 3, for offspring multi-locus genotypes).
aAllelic sizes have LiCor primer tails.
| Number | Date | Country | |
|---|---|---|---|
| 20080141406 P1 | Jun 2008 | US |
