Low Hemoglobin Concentration Cell Percentage and Method of Use in Detection of Iron Deficiency

Abstract

A method of producing low hemoglobin concentration cell percentage (LHCC %) and the method of use for detection of iron deficiency have been disclosed. LHCC % is a sigmoid function of mean cell hemoglobin concentration (MCHC), which correlates linearly with hypochromic red cell percentage.

Description

BRIEF DESCRIPTION OF DRAWINGS

FIG. 1A is a graphic illustration of red blood cell distribution and the distribution of hypochromic red blood cells.

FIG. 1B is a graphic illustration of red blood cell distribution and the distribution of the low hemoglobin concentration cells represented by the sigmoid function of MCHC of the present invention.

FIG. 2 is the ROC curve of MCHC, as described in the Example.

FIG. 3A shows the correlation of MCHC vs. % Hypo, as described in the Example.

FIG. 3B shows the correlation of MCHC vs. % Hypo of FIG. 3A with an expanded scale in y-axis.

FIG. 4A shows the correlation of LHCC % vs. % Hypo, as described the Example.

FIG. 4B shows the correlation of the enhanced LHCC % vs. % Hypo, as described in the Example.

FIG. 5A is the ROC curve of LHCC %, as described in the Example.

FIG. 5B is the ROC curve of enhanced LHCC %, as described in the Example.

Claims

1. A method of producing a low hemoglobin concentration cell percentage (LHCC %) of a blood sample on a hematology analyzer comprising: (a) mixing a first aliquot of a blood sample with a blood diluent to form a first sample mixture, analyzing said first sample mixture on said hematology analyzer, and obtaining a mean cell volume of red blood cells (MCV) and a red blood cell concentration (RBC);(b) mixing a second aliquot of said blood sample with a reagent system to form a second sample mixture, analyzing said second sample mixture on said hematology analyzer, and obtaining a hemoglobin concentration (Hgb) of said blood sample;(c) obtaining mean cell hemoglobin concentration (MCHC) using said MCV, said RBC and said Hgb;(d) obtaining said low hemoglobin concentration cell percentage (LHCC %) as a function of said MCHC; and(e) reporting said LHCC % of said blood sample on said hematology analyzer.

2. The method of claim 1, wherein said LHCC % is a sigmoid function of said MCHC.

3. The method of claim 1, wherein said LHCC % is defined by following equation: LHCC %=1/(1+(EXP(d*(q−MCHC))))*100.wherein d and q are constants.

4. The method of claim 3, wherein said d and q are 1.8 and 30, respectively.

5. The method of claim 1, wherein said LHCC % is an enhanced LHCC % defined by following equation: Enhanced LHCC %=SQRT((1−(1/(1+(EXP(d*(q−MCHC)))))))*100.wherein d and q are constants.

6. The method of claim 5, wherein said d and q are 1.8 and 30, respectively.

7. The method of claim 1, wherein said low hemoglobin concentration cell percentage (LHCC %) correlates linearly with hypochromic red cell percentage.

8. The method of claim 1, wherein said low hemoglobin concentration cell percentage (LHCC %) is obtained by a non-linear resealing of said MCHC.

9. A method of detection of iron deficiency comprising the steps of: (a) analyzing a blood sample on a hematology analyzer and obtaining mean cell hemoglobin concentration (MCHC) of red blood cells;(b) obtaining a low hemoglobin concentration cell percentage (LHCC %) defined as a function of said MCHC;(c) comparing said LHCC % to a predetermined iron deficiency criterion; and(d) reporting an indication of iron deficiency if said LHCC % meets said predetermined iron deficiency criterion.

10. The method of claim 9, wherein said LHCC % is a sigmoid function of said MCHC.

11. The method of claim 9, wherein said LHCC % is defined by following equation: LHCC %=1/(1+(EXP(d*(q−MCHC))))*100.wherein d and q are constants.

12. The method of claim 11, wherein said d and q are 1.8 and 30, respectively.

13. The method of claim 9, wherein said LHCC % is an enhanced LHCC % defined by following equation: Enhanced LHCC %=SQRT((1−(1/(1+(EXP(d*(q−MCHC)))))))*100.wherein d and q are constants.

14. The method of claim 13, wherein said d and q are 1.8 and 30, respectively.