Claims
- 1. A combinatorial method to prepare a library of peptide tracers for use in a luminescence-based assay having the formula
- 2. A library of labeled peptides synthesized by the method of claim 1.
- 3. The library of claim 2, wherein the number of labeled peptides in the library is at least 96.
- 4. The library of claim 3, wherein the number of labeled peptides in the library is at least 384.
- 5. The method of claim 1, wherein the peptide tracers are prepared in parallel.
- 6. The method of claim 1, wherein the solid phase supports are wells of a microplate.
- 7. The method of claim 1, further comprising
cleaving the peptide tracers from the solid phase supports.
- 8. The method of claim 1, the luminescence-based assay involving binding between at least one of the peptide tracers and a target, further comprising
identifying any peptide tracers that bind to the target.
- 9. The method of claim 8, wherein the step of identifying any peptide tracers that bind includes the steps of mixing a multiplicity of labeled peptide tracers with the target.
- 10. The method of claim 8, wherein the step of identifying any peptide tracers that bind includes the steps of
exposing the solid phase supports to a target labeled with a long-lifetime emission luminophore (FL-L), subjecting the solid phase support to polarized light comprising a wavelength for excitation of the FL-L so as to effect emission of polarized light, measuring the degree of polarization of the emitted light at each defined location on the solid support, and identifying as a peptide tracer useful in luminescence polarization assays for the target, a peptide tracer at a solid phase support having a low degree of measured polarization.
- 11. The method of claim 10, further comprising
labeling the identified successful peptide tracer with a short-lifetime luminophore before or after cleaving the tracer from the solid phase support.
- 12. A library of luminescent peptide tracers comprising a multiplicity of peptides of the formula
- 13. The library of claim 12, wherein the number of labeled peptides in the library is at least 96.
- 14. The library of claim 13, wherein the number of labeled peptides in the library is at least 384.
- 15. A method to identify a tracer for use in a luminescence polarization assay for a target which tracer is the opposite member of a specific binding pair comprising the target which method comprises
placing candidate tracers at separate, defined locations on a solid support, exposing the locations on the support to a target labeled with a long-lifetime emission luminophore (FL-L), subjecting the solid support to polarized light comprising a wavelength for excitation of the FL-L so as to effect emission of polarized light, measuring the degree of polarization of the emitted light at each defined location on the solid support, and identifying as a tracer useful in luminescence polarization assays for the target, a tracer at a defined location having a low degree of measured polarization.
- 16. The method of claim 15 further comprising
labeling the identified successful tracer with a short-lifetime luminophore before or after cleaving the tracer from the solid support.
- 17. The method of claim 15, wherein the locations on solid support are wells of a microplate.
- 18. The method of claim 15, wherein the displaying of candidate tracers is accomplished by synthesis of the tracers at the various locations on the solid support.
CROSS-REFERENCES TO RELATED APPLICATIONS
[0001] This application is a continuation of PCT Patent Application Ser. No. PCT/US00/15774, filed Jun. 9, 2000, which is incorporated herein by reference.
[0002] This application is based upon and claims priority under 35 U.S.C. §119 from the following U.S. Provisional Patent Application, which is incorporated herein by reference: Ser. No. 60/138,311, filed Jun. 9, 1999.
[0003] This application incorporates by reference the following U.S. patent applications: Ser. No. 08/840,553, filed Apr. 14, 1997; Ser. No. 08/929,095, filed Sep. 15, 1997; Ser. No. 09/118,141, filed Jul. 16, 1998; Ser. No. 09/144,575, filed Aug. 31, 1998; Ser. No. 09/144,578, filed Aug. 31, 1998; Ser. No. 09/146,081, filed Sep. 2, 1998; Ser. No. 09/156,318, filed Sep. 18, 1998; Ser. No. 09/160,533, filed Sep. 24, 1998; Ser. No. 09/302,158, filed Apr. 29, 1999; Ser. No. 09/349,733, filed Jul. 8, 1999; Ser. No. 09/468,440, filed Dec. 21, 1999; Ser. No. 09/478,819, filed Jan. 5, 2000; Ser. No. 09/494,407, filed Jan. 28, 2000; and Ser. No. 09/556,030, filed Apr. 20, 2000.
[0004] This application also incorporates by reference the following PCT patent applications: Ser. No. PCT/US99/01656, filed Jan. 25, 1999; Ser. No. PCT/US99/03678, filed Feb. 19, 1999; Ser. No. PCT/US99/08410, filed Apr. 16, 1999; Ser. No. PCT/US99/16057, filed Jul. 15, 1999; Ser. No. PCT/US99/16453, filed Jul. 21, 1999; Ser. No. PCT/US99/16621, filed Jul. 23, 1999; Ser. No. PCT/US99/16286, filed Jul. 26, 1999; Ser. No. PCT/US99/16287, filed Jul. 26, 1999; Ser. No. PCT/US99/24707, filed Oct. 19, 1999; Ser. No. PCT/US00/00895, filed Jan. 14, 2000; Ser. No. PCT/US00/03589, filed Feb. 11, 2000; Ser. No. PCT/US00/04543, filed Feb. 22, 2000; Ser. No. PCT/US00/06841, filed Mar. 15, 2000; Ser. No. PCT/US00/12277, filed May 3, 2000; Ser. No. PCT/US00/16025, filed Jun. 9, 2000; and Ser. No. PCT/US00/16012, filed Jun. 9, 2000.
[0005] This application also incorporates by reference the following U.S. provisional patent applications: Ser. No. 60/138,438, filed Jun. 10, 1999; Ser. No. 60/138,737, filed Jun. 11, 1999; Ser. No. 60/138,893, filed Jun. 11, 1999; Ser. No. 60/142,721, filed Jul. 7, 1999; Ser. No. 60/143,185, filed Jul. 9, 1999; Ser. No. 60/153,251, filed Sep. 10, 1999; Ser. No. 60/164,633, filed Nov. 10, 1999; 60/165,813, filed Nov. 16,1999; Ser. No. 60/167,301, filed Nov. 24, 1999; Ser. No. 60/167,463, filed Nov. 24, 1999; Ser. No. 60/178,026, filed Jan. 26, 2000; Ser. No. 60/182,036, filed Feb. 11, 2000; Ser. No. 60/182,419, filed Feb. 14, 2000; Ser. No. 60/184,719, filed Feb. 24, 2000; Ser. No. 60/184,924, filed Feb. 25, 2000; Ser. No. 60/190,265, filed Mar. 17, 2000; Ser. No. 60/191,890, filed Mar. 23, 2000; Ser. No. 60/193,586, filed Mar. 30, 2000; Ser. No. 60/197,324, filed Apr. 14, 2000; Ser. No. 60/200,530, filed Apr. 27, 2000; Ser. No. 60/200,594, filed Apr. 28, 2000; and Ser. No. 60/202,087, filed May 4, 2000.
[0006] This application also incorporates by reference the following publications: K. E. van Holde, Physical Biochemistry (2nd ed. 1985); William Bains, Biotechnology from A to Z (1993); Richard P. Haugland, Handbook of Fluorescent Probes and Research Chemicals (6th ed. 1996); Joseph R. Lakowicz, Principles of Fluorescence Spectroscopy (2nd ed. 1999); Bob Sinclair, Everything's Great When It Sits on a Chip: A Bright Future for DNA Arrays, 13 THE SCIENTIST, May 24, 1999, at 18; and Charles R. Cantor and Paul R. Schimmel, Biophysical Chemistry (1980).
Provisional Applications (1)
|
Number |
Date |
Country |
|
60138311 |
Jun 1999 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
PCT/US00/15774 |
Jun 2000 |
US |
Child |
09770724 |
Jan 2001 |
US |