Claims
- 1. A combination comprising a plurality of cDNAs having the nucleic acid sequences of SEQ ID NOs:1-9 and a cDNA encoding pepsin C and the complements of these cDNAs.
- 2. An isolated cDNA comprising a nucleic acid sequence selected from SEQ ID NOs:1-9 and the complements thereof.
- 3. A method for using a combination comprising a plurality of cDNAs to detect expression of a nucleic acid in a sample comprising:
a) hybridizing the combination of claim 1 to nucleic acids of the sample under conditions to form at least one hybridization complex; and b) detecting hybridization complex formation, wherein complex formation indicates expression of the nucleic acid in the sample.
- 4. The method of claim 3 further comprising amplifying the nucleic acids of the sample prior to hybridization.
- 5. The method of claim 3 wherein the sample is from lung.
- 6. The method of claim 3 wherein complex formation is compared to standards and indicates differential expression.
- 7. The method of claim 6 wherein differential expression is diagnostic of a lung disorder.
- 8 A substrate upon which the combination of claim 1 is arrayed.
- 9. A method of using a combination to screen a plurality of molecules to identify at least one ligand which specifically binds a cDNA of the combination, the method comprising:
a) contacting the substrate of claim 8 with molecules under conditions to allow specific binding; and b) detecting specific binding, thereby identifying a ligand which specifically binds a cDNA of the combination.
- 10. The method of claim 9 wherein the molecules to be screened and purified are selected from DNA molecules, RNA molecules, peptides, and proteins.
- 11. A composition comprising a cDNA of claim 2 and a labeling moiety.
- 12. A vector comprising a cDNA of claim 2.
- 13. A host cell comprising the vector of claim 12.
- 14. A method for using a host cell to produce a protein, the method comprising:
a) culturing the host cell of claim 13 under conditions for expression of the protein; and b) recovering the protein from cell culture.
- 15. A purified protein obtained using the method of claim 14.
- 16. A composition comprising the protein of claim 15 and a pharmaceutical carrier.
- 17. A method for using a protein to screen a plurality of molecules to identify at least one ligand which specifically binds the protein, the method comprising:
a) combining the protein of claim 15 with the plurality of molecules under conditions to allow specific binding; and b) detecting specific binding, thereby identifying a ligand which specifically binds the protein.
- 18. The method of claim 17 wherein the plurality of molecules is selected from antibodies, DNA molecules, RNA molecules, proteins, and small drug molecules.
- 19. A method of using a protein to prepare and purify an antibody comprising:
a) immunizing an animal with the protein of claim 15 under conditions to elicit an antibody response; b) isolating animal antibodies; c) attaching the protein to a substrate; d) contacting the substrate with isolated antibodies under conditions to allow specific binding to the protein; e) dissociating antibody from the protein, thereby obtaining purified antibody.
- 20. An antibody produced by the method of claim 19.
Parent Case Info
[0001] This application claims benefit of provisional application Serial No. 60/317,822, filed Sep. 7, 2001.
Provisional Applications (1)
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Number |
Date |
Country |
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60317822 |
Sep 2001 |
US |