Claims
- 1. A transgenic ungulate comprising a transgene encoding lysozyme, wherein said transgenic ungulate comprises a non-functional β-lactoglobulin allele, wherein said ungulate is female, and wherein said lysozyme-encoding transgene is expressed in mammary gland cells of said ungulate, wherein milk produced by said transgenic ungulate has a level of lysozyme that is at least about 10% higher than the level of lysozyme in milk of a non-transgenic ungulate of the same species, and wherein the milk has a level of β-lactoglobulin that is at least about 10% lower that the level of β-lactoglobulin in milk of a non-transgenic ungulate of the same species.
- 2. The transgenic ungulate of claim 1, wherein said transgene comprises a coding sequence for lysozyme operably linked to a mammary specific promoter.
- 3. The transgenic ungulate of claim 1, wherein said ungulate is a cow.
- 4. The transgenic ungulate of claim 1, wherein said ungulate is a goat.
- 5. The transgenic ungulate of claim 1, wherein said transgene is chromosomally integrated.
- 6. The transgenic ungulate of claim 1, wherein said ungulate is heterozygous for the transgene and heterozygous for the non-functional β-lactoglobulin allele.
- 7. The transgenic ungulate of claim 1, wherein said ungulate is homozygous for the transgene and homozygous for the non-functional β-lactoglobulin allele.
- 8. The transgenic ungulate of claim 1, wherein the transgene encodes human lysozyme.
- 9. An isolated fertilized egg, wherein said egg is isolated from a transgenic ungulate comprising a transgene encoding lysozyme and comprising a non-functional β-lactoglobulin allele.
- 10. A composition comprising an isolated fertilized egg according to claim 9; and a cryoprotective agent.
- 11. A method of producing a food product, said method comprising harvesting a food product from a transgenic ungulate of claim 1.
- 12. A method of producing a food product, the method comprising processing a food product harvested from a transgenic ungulate of claim 1.
- 13. A method of producing cheese, the method comprising:
a) adding rennet to milk harvested from a transgenic ungulate according to claim 1;b) allowing curd formation to occur; and c) producing cheese from the curds.
- 14. A milk product produced by a transgenic ungulate of claim 1, wherein said milk product has a level of lysozyme that is at least about 10% higher than the level of lysozyme in milk of a non-transgenic ungulate of the same species, and wherein said milk product has a level of β-lactoglobulin that is at least about 10% lower than the level of β-lactoglobulin in milk of a non-transgenic ungulate of the same species.
- 15. The milk product of claim 14, wherein the milk product has reduced rennet clotting time compared to a milk product of a non-transgenic animal of the same species.
- 16. A processed milk produced from milk of a transgenic ungulate of claim 1.
- 17. The processed milk product of claim 16, wherein the processed milk product is cheese.
- 18. The processed milk product of claim 17, wherein the cheese has increased gel strength compared to cheese made from milk of a non-transgenic ungulate of the same species.
CROSS-REFERENCE
[0001] This application claims the benefit of U.S. Provisional Patent Application No. 60/439,150 filed Jan. 9, 2003, and of U.S. Provisional Patent Application No. 60/480,356 filed Jun. 20, 2003, which applications are incorporated herein by reference in their entirety.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60480356 |
Jun 2003 |
US |
|
60439150 |
Jan 2003 |
US |