Claims
- 1. An isolated polypeptide having CDP-diacylglycerol synthase (CDS) activity comprising the amino acid sequence of SEQ ID NO. 2 and enzymatically active fragments thereof.
- 2. The polypeptide of claim 1, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO. 2.
- 3. A method of screening one or more agents for the ability to inhibit or stimulate CDS enzymatic activity in a cell-free or cell-based assay, comprising:(a) contacting the CDS polypeptide of claim 1, or contacting a cell that expresses said polypeptide, with said one or more agents under assay conditions suitable for the detection of said enzymatic activity; and (b) measuring whether said enzymatic activity is thereby inhibited or stimulated by said one or more agents, wherein:said agents are selected from compounds, compositions, antibodies or antibody fragments, antisense sequences and ribozyme nucleotide sequences for CDS.
- 4. The method of claim 3, wherein said one or more agents is selected from a combinatorial chemical library.
- 5. A method of expressing the polypeptide of claim 1, comprising:(a) introducing into a cell a polynucleotide comprising a nucleotide sequence selected from the group consisting of: (i) the DNA sequence of SEQ ID NO. 1 and fragments thereof that encode CDS polypeptides with enzymatic activity; and (ii) a cDNA sequence which, due to the degeneracy of the genetic code, encodes a polypeptide of SEQ ID NO. 2 and enzymatically active fragments thereof, wherein:said polynucleotide is operably linked to a promoter; and (b) culturing said cell under conditions that result in the expression of said polypeptide.
Parent Case Info
This application is a divisional of application Ser. No. 08/672,814, filed on Jun. 28, 1996, now U.S. Pat. No. 5,952,480.
Non-Patent Literature Citations (4)
Entry |
Hancock, A. M., et al. (1996) J. Neurochem. 67(5), 2200-2203. |
Saito, S., et al. (1997) J. Biol. Chem. 272(14), 9503-9509. |
Icho, T., et al. (1985) J. Biol. Chem. 260(22), 12078-12083. |
Wallace (1993) Met. Neurosci. 18, 4957. |