Claims
- 1. A mammalian culture medium comprising recombinant human albumin and a medium that can support cell development, wherein the mammalian culture medium increases the viability of gametes or embryonic cells cultured in the mammalian culture medium, and further wherein the mammalian culture medium is free from non-recombinant human albumin.
- 2. The culture medium according to claim 1, wherein the medium that can support cell development is selected from the group consisting of G1.2/G2.2, KSOM/KSOMaa, M16, SOF/SOFaa, MTF, P1, HTF, Earle's, Hams F-10, M2, Hepes-G1.2, Whitten's and PBS.
- 3. The culture medium according to claim 1, wherein the medium that can support cell development supports embryo development.
- 4. The culture medium according to claim 1, wherein the medium that can support cell development supports mammalian stem cell development.
- 5. The culture medium according to claim 1, comprising about 0.5 mg/ml to about 5.0 mg/ml recombinant human albumin and further comprising citrate.
- 6. The culture medium according to claim 2, further comprising citrate.
- 7. The culture medium according to claim 1, comprising about 0.5 mg/ml to about 5.0 mg/ml recombinant human albumin and further comprising fermented hyaluronan.
- 8. The culture medium according to claim 2, further comprising fermented hyaluronan.
- 9. The culture medium according to claim 3, further comprising citrate.
- 10. The culture medium according to claim 4, further comprising citrate.
- 11. The culture medium according to claim 3, further comprising fermented hyaluronan.
- 12. The culture medium according to claim 4, further comprising fermented hyaluronan.
- 13. A method of increasing the viability of embryonic cells comprising culturing an embryo in the mammalian culture medium of claim 1, wherein the viability of the embryo is increased.
- 14. A mammalian culture medium supplement comprising recombinant human albumin, wherein the supplement increases the viability of gametes or embryonic cells cultured in a medium containing the supplement, and further wherein the supplement is free from non-recombinant human albumin.
- 15. The supplement according to claim 14 further comprising citrate.
- 16. The supplement according to claim 15, wherein the citrate is present in a range of about 0.1 mM to about 1.0 mM when added to the medium.
- 17. The supplement according to claim 14, wherein the recombinant human albumin is present in a range of about 0.5 mg/ml to about 5.0 mg/ml when added to the medium.
- 18. A method of producing a supplement for a mammalian culture medium comprising adding recombinant human albumin to either water, saline or medium to make a supplement for a mammalian culture medium, wherein the supplement increases the viability of gametes or embryonic cells cultured in a medium containing the supplement, and further wherein the supplement is free from non-recombinant human albumin.
- 19. The method of producing a supplement for a mammalian culture medium of claim 18 further comprising adding citrate.
- 20. A kit for supplementation of mammalian culture medium, comprising:
(a) a medium comprising recombinant human albumin, and optionally one or more ingredients selected from the group consisting of mammalian culture medium, fermented hyaluronan, citrate and combinations thereof, wherein the medium increases the viability of gametes or embryonic cells cultured in the medium, and further wherein the medium is free from non-recombinant human albumin; and (b) instructions for use of the kit.
CROSS-REFERENCE TO RELATED APPLICATION
[0001] This application is a continuation of U.S. patent application Ser. No. 09/877,395, filed Jun. 8, 2001, which claims priority from U.S. provisional patent application No. 60/210,649, filed Jun. 9, 2000, and from U.S. provisional patent application No. 60/212,232, filed Jun. 16, 2000.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60210649 |
Jun 2000 |
US |
|
60212232 |
Jun 2000 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
09877395 |
Jun 2001 |
US |
Child |
10764821 |
Jan 2004 |
US |