Claims
- 1. A recombinant polynucleotide selected from the group consisting of:
(a) a polynucleotide encoding a polypeptide having an amino acid sequence of SEQ ID NO:3. (b) a polynucleotide having the nucleotide sequence of SEQ ID NO:1, (c) a polynucleotide which is complementary to the polynucleotide of (a) or (b), and (d) a polynucleotide that hybridizes with a polynucleotide of (a), (b), or (c) under stringent conditions.
- 2. The polynucleotide of claim 1 wherein the polynucleotide comprises nucleotides selected from the group consisting of natural, non-natural and modified nucleotides.
- 3. The polynucleotide of claim 1 wherein the internucleotide linkages are selected from the group consisting of natural and non-natural linkages.
- 4. An expression vector comprising a polynucleotide of claim 1.
- 5. A host cell comprising the expression vector of claim 4.
- 6. A process for expressing a mSPP1 protein from a recombinant host cell, comprising:
(a) transforming a suitable host cell with an expression vector of claim 4; and, (b) culturing the host cell of step (a) in conditions under which allow expression of said the mSPP1 protein from said expression vector.
- 7. A recombinant polypeptide having an amino acid sequence selected from the group consisting of
(a) a polypeptide having an amino acid sequence of SEQ ID NO:3, (b) a polypeptide having an amino acid sequence of SEQ ID NO:4.
- 8. A method of determining whether a candidate compound is an inhibitor of a mSPP1 polypeptide comprising:
(a) providing at least one host cell harboring an expression vector that includes a polynucleotide selected from the group consisting of: (i) a polynucleotide encoding a polypeptide having an amino acid sequence of SEQ ID NO:3, and (ii) a polynucleotide having the coding sequence from SEQ ID NO:1, (b) contacting at least one of said cells with the candidate to permit the interaction of the candidate with the mSPP1 polypeptide, and (c) determining whether the candidate is an inhibitor of the mSPP1 polypeptide by ascertaining the relative activity of the polypeptide in the presence of the candidate.
- 9. The method of claim 8 wherein in step (c) the relative activity is determined by comparing a measurement of mSPP1 polypeptide activity of at least one cell before step (b) to a measurement of mSPP1 polypeptide activity of at least one cell after step (b).
- 10. The method of claim 8 further comprising a control assay using a mSPP1b protein.
- 11. A method of determining whether a candidate compound is an inhibitor of a mSPP1 polypeptide comprising:
(a) providing a sample that includes a mSPP1 polypeptide having an amino acid sequence of SEQ ID NO:3, (b) contacting said sample with the candidate to permit the interaction of the candidate with the mSPP1 polypeptide, and (c) determining whether the candidate is an inhibitor of the mSPP1 polypeptide by ascertaining the relative activity of the mSPP1 polypeptide in the presence of the candidate.
- 12. The method of claim 11 wherein the polypeptide has the amino acid sequence of SEQ ID NO:3.
- 13. The method of claim 11 wherein in step (c) the relative activity is determined by comparing a measurement of mSPP1 polypeptide activity of the sample before step (b) to a measurement of mSPP1 polypeptide activity of the sample after step (b)
- 14. A transgenic animal lacking a functional endogenous mSPP1 gene.
- 15. The animal of claim 14 further comprising a non-native mSPP1 gene.
- 16. A recombinant polynucleotide selected from the group consisting of:
(a) a polynucleotide encoding a polypeptide having an amino acid sequence of SEQ ID NO:4. (b) a polynucleotide having the nucleotide sequence of SEQ ID NO:2, (c) a polynucleotide which is complementary to the polynucleotide of (a) or (b), and (d) a polynucleotide that hybridizes with a polynucleotide of (a), (b), or (c) under stringent conditions.
- 17. An expression vector comprising a polynucleotide of claim 16.
- 18. A host cell comprising the expression vector of claim 17.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application claims the benefit of U.S. Provisional Application No. 60/180,534 filed Feb. 7, 2000, the contents of which are incorporated herein by reference in their entirety.
Provisional Applications (1)
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Number |
Date |
Country |
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60180534 |
Feb 2000 |
US |