Patent Application
20250042948
The present invention relates to a manufacturing process to produce Linaclotide and its acetate salt, more particularly, to obtain amorphous High-Purity Linaclotide or its acetate salt with a chromatographic purity equal to or higher than 99.9% and a content of IMD-Linaclotide and Cys-1-α-ketone-Linaclotide impurities each one lower than 100 ppm, preferably lower than 50 ppm, more preferably lower than 40 ppm.
The invention further relates to an analytical method able to detect IMD-Linaclotide and
Cys-1-α-ketone Linaclotide even at 40 ppm level (LOD) and quantify IMD-Linaclotide and Cys-1-α-ketone Linaclotide even at 50 ppm level (LOQ), by Ion-Pairing Chromatography (IPC).
Linaclotide acetate is the Drug Substance contained in the commercial products Linzess® for the US market and Constella® for the EU market, in the dosage forms of 72, 145 and 290 mcg capsules.
The Active Pharmaceutical Ingredient is a complex Cys-rich cyclic peptide consisting of 14 aminoacids and containing three regiospecific disulfide bonds between Cys [1-6], [2-10], [5-13] as illustrated in the Formula (I):
This drug belongs to the class of agonists of guanylate cyclase and acts locally on guanylate cyclase subtype C receptors located in the internal surface of intestinal epithelium. The activation of guanylate cyclase by Linaclotide leads to increased levels of cGMP which in turn activates a secretion of chloride and hydrogenocarbonate into the intestinal lumen, increasing intestinal fluid secretion and accelerating transit.
The structure of Linaclotide was reported for the first time in U.S. Pat. No. 7,304,036, where it is generically described that the production of the peptide can be achieved by fermentation of appropriately modified bacterial vectors or synthetically by Solid Phase Peptide Synthesis (SPPS), but a complete description of the chemical procedure is not given, while the first detailed synthetic method published for Linaclotide is found in Peptide Science 96 (1), 69-80 (2010), where SPPS manufacturing process is reported and oxidative methods for the disulfide bridges formation are proposed.
Chemical preparative procedures of Linaclotide are described, for example, in WO2014188011 (Lonza), where the linear backbone peptide on resin is prepared by SPPS through a one-by-one aminoacid assembling strategy, then the linear peptide is cleaved from the resin and concurrently deprotected; the three disulfide bridges are formed according to random strategy by oxidation with air in dimethylsulphoxide and the crude Linaclotide is then purified by reverse phase chromatography, to be finally isolated in an undisclosed grade of purity by lyophilization from a 50% tert-butanol aqueous solution.
WO2015022575 (Auro Peptides) also describes a chemical synthesis procedure where two fragments are prepared and coupled by a SPPS stepwise strategy to obtain the linear backbone peptide on resin, which is then cleaved from the resin and concurrently deprotected, cyclized by random strategy with air and an oxidizing agent (e.g. hydrogen peroxide) to form the three disulfide bridges and finally purified and lyophilized giving Linaclotide having an HPLC purity, at most, of 98.9%.
WO2016038497 (Auro Peptides) furthermore, reports a method for preparing Linaclotide where SPPS is applied through a one-by-one aminoacid assembling strategy, to generate the linear backbone peptide on resin, which is then freed from the resin and deprotected (concurrently or sequentially), cyclized by random strategy with air and an oxidizing agent (e.g. hydrogen peroxide) and finally purified by reverse phase chromatography and lyophilized to yield a final product having an HPLC purity of 98.9% or a generic HPLC purity of >99%, without details of the structure of the impurities present in the product.
WO2017101810 (Hybio Pharmaceuticals), describes a regioselective synthesis of Linaclotide consisting in the preliminary preparation of the linear backbone peptide on resin by SPPS via one-by-one aminoacid assembling strategy, the oxidative formation of the first disulfide bridge [1-6] on the peptide still linked to the resin, the oxidative formation of the second disulfide bridge [2-10] in solution, and finally the oxidative formation of the third disulfide bridge [5-13] after deprotection of the two methylated cysteines.
Crude Linaclotide is purified by reverse phase chromatography and then lyophilized. Although it is described that the synthesis proceeds in a regioselective way, the oxidative nature of the reactions used to create the necessary disulfide bonds does not allow to avoid the formation of dimers and multimer impurities of the peptide. In addition, the use of hardly industrially adequate solvents such as diethyl ether is also described, as well as an HPLC purity of the final Linaclotide of 99.5%, with no mention of the content for example of the IMD-Linaclotide and Cys-1-α-ketone-Linaclotide impurities. However, an additional purification by reverse phase chromatography of the intermediate bis-disulfide peptide is described.
WO2017134687 (Cipla) also describes a preparation of Linaclotide by initial manufacturing of the linear backbone peptide on resin by SPPS via one-by-one aminoacid assembling strategy, then a concurrent cleavage from the resin and removal of the S-phenylacetamidomethyl protecting groups follow, to finally oxidize the product in aqueous solvent via random strategy, purify it by reverse phase chromatography and lyophilize it to generate a final product having a generic HPLC purity of >99%, free from dimers and multimer impurities.
WO2019113872 (Shenzhen) details as well a manufacturing method of Linaclotide through the preparation of the linear backbone peptide on resin either by SPPS via one-by-one aminoacid assembling strategy or by stepwise strategy, which once obtained is not cleaved from the resin but cyclized by oxidation via N-alogenyl sucinimmide; crude Linaclotide on resin is then detached, purified by reverse phase chromatography and lyophilized.
With reference to processes for the preparation of Linaclotide used in the state of the art, the inventors have observed that these processes do not provide amorphous Linaclotide having high purity of at least 99.9% and a content of IMD-Linaclotide and Cys-1-α-ketone-Linaclotide impurities each one lower than 100 ppm, preferably lower than 50 ppm, more preferably lower than 40 ppm.
The present inventors have unexpectedly found a simplified, industrially applicable and robust process for preparing Linaclotide and its acetate salt, wherein the peptide is totally prepared by Liquid Phase Peptide Synthesis (LPPS) and without any intermediate purification. More particularly, the method allows to produce an amorphous Linaclotide or its acetate salt characterized by chromatographic purity equal to or higher than 99.9% and particularly having a content of IMD-Linaclotide and Cys-1-α-ketone-Linaclotide impurities each one lower than 100 ppm, preferably lower than 50 ppm (LOQ of the Ion-Pairing analytical method) and more preferably lower than 40 ppm (LOD of the Ion-Pairing analytical method).
Unless otherwise defined, all terms of art, notations and other scientific terminology used herein are intended to have the meanings commonly understood by those of skill in the art to which this disclosure pertains. In some cases, terms with commonly understood meanings are defined herein for clarity and/or for ready reference; thus, the inclusion of such definitions herein should not be construed to represent a substantial difference over what is generally understood in the art.
The terms “approximately” and “about” herein refer to the range of the experimental error, which may occur in a measurement.
The term “High-Purity” herein refer to a chromatographic purity equal to or higher than 99.9% and a content of IMD-Linaclotide and Cys-1-α-ketone-Linaclotide impurities each one lower than 100 ppm, preferably lower than 50 ppm, more preferably lower than 40 ppm.
The terms “HPLC purity” or “chromatographic purity” herein refers to area under the curve in the chromatogram.
References herein to percent (%) purity are based on chromatographic purity (chromatographic area %).
The terms “comprising”, “having”, “including” and “containing” are to be construed open-ended terms (i.e. meaning “including, but not limited to”) and are to be considered as providing support also for terms as “consist essentially of”, “consisting essentially of”, “consist of” or “consisting of”.
The terms “consist essentially of”, “consisting essentially of” are to be construed as semi-closed terms, meaning that no other ingredients which materially affects the basic and novel characteristics of the invention are included (optional excipients may thus included).
The terms “consists of”, “consisting of” are to be construed as closed terms.
In a first aspect, the present invention relates to a liquid-phase process for preparing Linaclotide or its acetate salt comprising the following generic steps:
This process is a notable improvement with respect to the prior art and its advantages are summarized below:
According to a second aspect thereof, the present invention relates to amorphous Linaclotide or its acetate salt having a chromatographic purity equal to or higher than 99.9% and an amount of any of the following impurities lower than 100 ppm, preferably lower than 50 ppm, more preferably lower than 40 ppm:
Preferably, the Linaclotide or its acetate salt has a content of multimers equal to or lower than 0.1% area %.
According to a third aspect thereof, the present invention relates to an analytical Ion-Pairing Chromatography (IPC) method to analyze Linaclotide or its acetate salt, which is able to detect (with a LOD 40 ppm) and quantify (with LOQ 50 ppm) trace quantities of the impurities IMD-Linaclotide and Cys-1-α-ketone-Linaclotide in the amorphous High-Purity Linaclotide. The structures of IMD-Linaclotide and Cys-1-α-ketone-Linaclotide impurities are reported below:
An object of the present invention is a liquid-phase process for the production of Linaclotide, preferably in the form of the acetate salt, which comprises the following steps:
In a preferred embodiment of the process according to the invention, in step b) the secondary base is a secondary amine, preferably DEA, piperidine, piperazine or morpholine, and trifluoroacetic acid is at least 80% by volume in water.
In a preferred embodiment of the process according to the invention, step c) is performed by intramolecular nucleophilic substitution.
Preferably, step c) is performed in an hydroalcoholic buffer at pH comprised between 7 and 8.
In a further preferred embodiment, in step c) the crude Linaclotide is obtained in aqueous solution.
In a preferred embodiment of the process according to the invention, the coupling between (II) and (III) and/or the coupling between [7-14] and (IV) and/or the coupling between [6-14] and (V) and/or the coupling between [5-14] and (VI) is performed in the presence of ethyl-ciano-(hydroxyimino)-acetate also called Oxyma or its derivatives, such as PyOxim or TOTU, or 1,3-dimethylbarbituric acid derivatives, such as Oxyma-B.
In a further preferred embodiment of the process according to the invention, the coupling between (II) and (III) and/or the coupling between [7-14] and (IV) and/or the coupling between [6-14] and (V) and/or the coupling between [5-14] and (VI) is performed in organic polar solvents, preferably selected from DMF, NMP, ACN. In a further preferred embodiment of the process according to the invention, the coupling between (II) and (III) and/or the coupling between [7-14] and (IV) and/or the coupling between [6-14] and (V) and/or the coupling between [5-14] and (VI) is performed in a temperature range between −10° C. and 35° C., preferably between 0° C. and 25° C.
In a preferred embodiment, the purification step d) is performed through Reverse Phase High Performance Sample Displacement (RP-HPSD), Reverse Phase High Performance Liquid Chromatograpy (RP-HPLC) or combination thereof.
In a preferred embodiment of the purification step d), the eluent phase consists of an aqueous solution, a polar organic solvent or a mixture thereof, preferably the polar organic solvent is selected from trifluoroacetic acid, acetic acid, acetonitrile and a mixture thereof, optionally with the addition of a buffer, more preferably a phosphoric buffer.
In a preferred embodiment, the purified Linaclotide is in solution, preferably said solution comprises water, acetic acid and acetonitrile.
Preferably, the process according to the invention allows to obtain amorphous Linaclotide having a chromatographic purity equal to or higher than 99.9%.
The process according to the invention may further comprise an isolation step f) by lyophilization starting from a hydroalcoholic solution or from an aqueous suspension.
Preferably, the hydroalcoholic solution contains tert-butanol, water, acetic acid or mixture thereof.
Preferably, the aqueous suspension is obtained by evaporation of the purified Linaclotide solution, more preferably the aqueous suspension contains acetic acid.
A further object of the present invention is amorphous Linaclotide or its acetate salt having a chromatographic purity equal to or higher than 99.9% and an amount of any of the following impurities lower than 100 ppm, preferably lower than 50 ppm (LOQ), more preferably lower than 40 ppm (LOD):
Preferably, amorphous High-Purity Linaclotide or its acetate salt has a content of multimers equal to or lower than 0.1% area %.
A further object of the present invention is a method for detect (LOD 40 ppm) and quantify (LOQ 50 ppm) the impurity content of IMD-Linaclotide and Cys-1-α-ketone in the product amorphous High-Purity Linaclotide or its acetate salt, comprising the elution of the product through an Ion-Pairing Chromatography (IPC) column, having a silica stationary phase containing alkyl chains, and an eluent phase consisting of an aqueous solution, a polar organic solvent, or a mixture thereof, optionally with the addition of a buffer, preferably a phosphoric buffer.
In a preferred embodiment of the method according to the invention, said alkyl chains are of octadecyl, octyl, or butyl (C18, C8 or C4) type, preferably C18.
Preferably, said polar organic solvent is selected from tetrahydrofuran, dioxane, dicloromethane, methanol, ethanol, n-propanol, isopropanol, butanol, pentane, hexane, toluene, trifluoroacetic acid, acetonitrile, or a mixture thereof; more preferably trifluoroacetic acid, acetonitrile, or a mixture thereof.
In a further preferred embodiment, the method according to the invention is characterized in that it is performed according to the following operating conditions:
The eluent phase of the method according to the invention contains an ion-pairing reagent, preferably heptanesulfonated salt.
In the manufacturing process which is an object of the present invention, crude Linaclotide is the first key intermediate, which is synthesized by a Liquid Phase Peptide Synthesis (LPPS)procedure, consisting of
One of the key points of the synthesis of a complex peptide with multidisulfide bridges such as Linaclotide is to drive the cyclization step in the right way, setting the conditions to obtain the correct regioisomer according to the desired disulfide mapping, avoiding the formation of misfolding impurities as well as the formation of multimers.
To reach this target, a non oxidative cyclization method has been developed where an intramolecular nucleophilic substitution in an hydroalcoholic buffer at pH comprised between 7-8 has been carried out involving three nucleophilic moieties (thiols group of free Cys) and three leaving groups (sulfonated groups on the remaining Cys).
A set of trials have been preliminarily executed with the aim of selecting the best locations for the three sulfonated Cys groups along the backbone; all the possible combinations of the linear advanced peptide have been chemically prepared and submitted to cyclization.
The best results in terms of chromatographic purity and assay of the desired Linaclotide final product have been obtained for the trial where the sulfonated groups were positioned on the Cys in position 1, 2 and 5, whereas all the other combinations have led to worst results, and in some case, even to a chromatographic complex pattern of regioisomers without a main peak.
Therefore surprisingly, the intramolecular nucleophilic substitution is not only an advantageous synthetic method for the formation of one disulfide bridge, but it is particularly efficient for the formation of more than one disulfide bridge (e.g. for the manufacturing of complex multidisulfide bridges peptide such as Linaclotide).
Another key point in the synthesis of a complex multidisulfide bridges peptide such as Linaclotide is the choice of the protecting groups on the remaining Cys that need to cyclize with the above described sulfonated Cys(in the specific invention in position 6, 10 and 13).
A set of trials have been preliminarly executed and the best results in terms of the final chromatographic purity, orthogonality, side-reactions and ease of handling have been obtained in the case of functionalization of the Cys in position 6 by Trt and of the Cys in position 10 and 13 by Cys-pseudoprolines.
The Cys-pseudoprolines represent a masked form of cysteine, having a five membered tiochetalic hindered ring with low tendency to racemization during coupling activation; the introduction of this moiety along the backbone has the additional advantage to guarantee higher solubility to the growing peptide as well to reduce the aggregation effects, leading to an improvement for the work-up.
The protection of the thiol groups of the Cys as pseudoprolines is a recent discovery and the relative uses in peptidic synthesis are still pioneristic, as of today, the available literature being related only to SPPS applications.
For this reason, the use of Cys-pseudoprolines in a LPPS manufacturing process as the one described hereby, represents an innovative application, considering also that the object of the synthesis is a complex Cys-rich peptide with multidisulfide bridges such as Linaclotide.
Once defined the positioning of the sulfonated groups along the linear protected peptide as well as the typology and the positioning of the protecting groups of the remaining Cys(which need to be deprotected before the cyclization step), a totally Liquid Phase Peptide Synthesis (LPPS) has been designed.
As preliminary synthetic steps to prepare the linear protected peptide, the following three fragments have to be synthesized, namely:
Each of the three fragments is characterized by a HPLC Purity not lower than 90%.
For the following step of preparation of the linear protected peptide, a convergent assembling of the Fragment A (VI), Fragment B (II), Fragment C (III) and the two aminoacid derivatives Fmoc-Cys(Trt)-OH (IV) in position 6 and Fmoc-Cys(SO3Na)-ONa (V) in position 5 is carried out, leading to the obtainment of the following 14-mer linear protected peptide (VII):
The assembling of Fragment A (VI), Fragment B (II), Fragment C (III) and the two aminoacids derivatives Fmoc-Cys(Trt)-OH (IV) in position 6 and Fmoc-Cys(SO3Na)-ONa (V) in position 5, to obtain linear protected peptide is carried out by the repetition of a simple, industrially applicable and robust synthetic protocol summarized in the below reported Scheme 2:
The linear protected peptide is characterized by an HPLC Purity not less than 65%.
In the following later step of preparation of the linear advanced intermediate, the removal of the protecting groups is carried on in 2 different steps, namely:
The linear advanced intermediate is characterized by an HPLC purity of at least 55%. In the final step to produce crude Linaclotide in solution, the cyclization of the linear advanced intermediate is carried out by a manufacturing method having the following characteristics:
Crude Linaclotide in solution is typically obtained with an HPLC Purity of 45-55%, which is particularly surprising and advantageous because such degree of purity is reached without any intermediate purification, allowing saving of time, lower costs and increase of productivity, as compared to existing methods.
Crude Linaclotide is further processed by the application of a combination of 2 purification techniques: Reverse Phase High Performance Sample Displacement (RP-HPSD) and Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) and is finally isolated by application of any of 2 different manufacturing options starting from Linaclotide purified solution (which is an ACN-water solution containing AcOH):
solvent switch from ACN to t-BuOH and lyophilization from t-BuOH-water (containing AcOH) solution
concentration and lyophilization from aqueous (containing AcOH) suspension according to the Flow Chart reported in Scheme 3:
Going into details, the step of chromatographic purification is carried out by application on the crude Linaclotide solution of a combination of 2 different techniques: RP-HPSD+RP-HPLC.
The RP-HPSD method works based on the concept that the sample molecules bind to the column and separate from one another depending on their affinities with the stationary phase. The technique foresees an overloading of the sample charge on the column and guarantees the separation of Linaclotide from its impurities by a mild slope gradient with a low % of organic solvent.
The overall chromatographic purification process can be represented in the Scheme 4 reported below:
At the end of the purification process, purified Linaclotide in solution is obtained having a chromatographic purity 99.9% minimum, starting from crude Linaclotide in solution (HPLC Purity about 45-55%). The residual amount of both IMD-Linaclotide and Cys-1-α-ketone-Linaclotide impurities does not exceed 100 ppm, each one being typically lower than 50 ppm (LOQ) and more typically lower than 40 ppm (LOD).
Once the purified Linaclotide solution is obtained, the final amorphous High-Purity Linaclotide can be isolated by application of one of the following alternative manufacturing options:
Linaclotide purified solution is loaded on a RP-column and a switch ACN→tBuOH is performed by eluting the column with a phase composed of a 1:1 mixture of Phase 1 (water-AcOH 100 mM) and Phase 2 (t-BuOH).
Then the t-BuOH-water (containing AcOH) solution is lyophilized to obtain amorphous High-Purity Linaclotide powder.
Linaclotide purified solution is concentrated under vacuum to remove ACN.
Then the obtained aqueous (AcOH) suspension is transferred into trays to be lyophilized to obtain amorphous High-Purity Linaclotide powder.
Amorphous High-Purity Linaclotide is characterized as follows:
A. Purity, Assay and evaluation of impurities IMD-Linaclotide and Cys-1-α-ketone-Linaclotide
The analytical method is based on the principles of the Ion-Pairing Chromatography (IPC): this technique allows the separation of ionic analytes using a mobile phase which contains a specific modifier consisting of lipophilic ions of opposite charge with respect to the analytes. The lipophilic ions of the mobile phase interact with the analytes balancing their ionic charges thus allowing separation on the stationary phase.
Optimization of the analytical variables allows also the separation of complex sample mixtures containing both ionic/ionizable species and neutral analytes.
The physical form of the amorphous High-Purity Linaclotide is evaluated by the following analyses:
The following examples are intended to further illustrate the invention but not limiting it.
STEP I—Synthesis of H-Glu(tBu)-Tyr(tBu)-OAll TFA salt
1240 grams of Trt-Glu(tBu)-OH DCHA salt were dissolved into 8.7 L of iPrOAc and the organic solution was washed with an aqueous solution of KHSO4, then concentrated and the residue dissolved in NMP (5.6 L).
H-Tyr(tBu)-OAll HCl salt (590 grams) was added and the coupling reaction carried out by PyOxim/NMM system
The reaction mixture was diluted with iPrOAc and the peptide rich organic solution was washed with an aqueous solution of NaHCOs and NaCl, then concentrated and the residue dissolved in DCM (6.1 L).
The acidic deprotection was carried out by a mix TFA-TIS.
The reaction solution was concentrated and precipitated by a mix n-heptane-DIPE, filtered, washed and dried.
H-Glu(tBu)-Tyr(tBu)-OAll TFA salt was obtained with Purity HPLC 99.8% and Yield 89%.
STEP II—Synthesis of Fmoc-Cys(SO3Na)-Cys(SO3Na)-Glu(tBu)-Tyr(tBu)-OH
952 grams of H-Glu(tBu)-Tyr(tBu)-OAll TFA salt and 810 grams of Fmoc-Cys(SO3Na)-ONa were dissolved in 4.8 L of DMF and the coupling reaction carried out by PyOxim/2,4,6 collidine system.
The reaction mixture was diluted with iPrOAc and the peptide rich organic solution was washed with an aqueous solution of KHSO4, NaHCO3 and NaCl, then concentrated and the residue dissolved in iPrOAc. The basic deprotection was carried out by DEA.
The reaction mixture was diluted with iPrOAc and the peptide rich organic solution was washed with an aqueous solution of NaHCO3, KHSO4 and NaCl, then concentrated, and the residue dissolved in DMF (7.9 L).
Fmoc-Cys(SO3Na)-ONa (695 grams) was added and the coupling reaction carried out by PyOxim/2,4,6 collidine system.
The reaction mixture was diluted with DCM and the peptide rich organic solution was washed with an aqueous solution of KHSO4, NaHCO3 and NaCl, then concentrated and the residue dissolved in DCM (19.7 L).
The allylester removal was carried out by PhSiHs and Pd(PPh3)4.
The reaction mixture was quenched with addition of an aqueous solution of NaCl, the phases were separated and to the organic phase an aqueous solution of NaHCO3 was added. The peptide was extracted from the aqueous phase by further addition of DCM. The organic phase was concentrated and precipitated by addition of MTBE, filtered, washed and dried.
Fmoc-Cys(SO3Na)-Cys(SO3Na)-Glu(tBu)-Tyr(tBu)-OH was obtained with Purity HPLC 94.3% and Yield 65%.
177 grams of H-Pro-OBzl HCl salt was suspended into 1.8 L of DMF. Fmoc-Asn(Trt)-OH (350 grams) was added and the coupling reaction carried out by PyOxim/2,4,6 collidine system.
The reaction mixture was precipitated by addition of an aqueous solution of NaHCO3, filtered, washed and dried.
The dried product was hydrogenated to remove benzylester protection using IPA (17.0 L) and water (1.0 L) as reaction solvents and Pd/C 5% (50% wet) (120 grams) as catalyst. The reaction was kept under H2 for not less than 3 h 50′. The suspension was filtered and the catalyst cake was washed by a mix IPA-water and the collected solutions were precipitated by charging an aqueous solution of KHSO4. The suspension was filtered, washed and dried.
Fmoc-Asn(Trt)-OH was obtained with Purity HPLC 97.0% and Yield 79%.
950 grams of Fmoc-Gly-Cys[Ψ(Dmp,H)pro]-OH and 582 grams of H-Tyr(tBu)-OtBu
HCl salt were dissolved into 4.8 L of NMP and the coupling reaction carried out by TOTU/NMM system.
The reaction mixture was precipitated by an aqueous solution of KHSO4, filtered, washed and dried.
Fmoc-Gly-Cys[Ψ(Dmp,H)pro]-Tyr(tBu)-OtBu was obtained with Purity HPLC 98.8% and Step Yield 90%
1277 grams of Fmoc-Gly-Cys[Ψ(Dmp,H)pro]-Tyr(tBu)-OtBu was dissolved into 5.7 L of ACN. The basic deprotection was carried out by DEA.
The reaction mixture was diluted with iPrOAc and the peptide rich organic solution was washed with an aqueous solution of KHSO4 and NaCl, then concentrated and the residue dissolved into iPrOAc.
The solution was precipitated by addition of n-heptane, filtered, washed and dried.
H-Gly-Cys[Ψ(Dmp,H)pro]-Tyr(tBu)-OtBu was obtained with Purity HPLC 95.1% and Yield 93%.
865 grams of H-Gly-Cys[Ψ(Dmp, H)pro]-OH and 572 grams of Fmoc-Thr (tBu)-OH were dissolved into 4.3 L of NMP and the coupling reaction carried out by PyOxim/NMM system.
The reaction mixture was diluted with IPA and precipitated by addition of an aqueous solution of NaHCO3, filtered, washed and dried.
Fmoc-Thr (tBu)-Gly-Cys[Ψ(Dmp, H)pro]-Tyr(tBu)-OtBu was obtained with Purity HPLC 93.9% and Yield 87%
1224 grams of Fmoc-Thr (tBu)-Gly-Cys[Ψ(Dmp,H)pro]-Tyr(tBu)-OtBu was dissolved into 6.1 L of iPrOAc. The basic deprotection was carried out by DEA.
The reaction mixture was diluted with iPrOAc and the peptide rich organic solution was washed with an aqueous solution of KHSO4 and NaCl, then concentrated, and the residue dissolved into NMP (6.1 L).
Fmoc-Ala-Cys[Ψ(Dmp,H)pro]-OH (688 grams) was added and the coupling reaction was carried out by TOTU/NMM system.
The reaction mixture was diluted with IPA and precipitated by an aqueous solution of NaHCO3, filtered and washed.
The product was then dried.
Fmoc-Ala-Cys[Ψ(Dmp,H)pro]-Thr (tBu)-Gly-Cys[Ψ(Dmp,H)pro]-Tyr(tBu)-OtBu was obtained with Purity HPLC 91.9% and Yield 93%
1476 grams of Fragment C was dissolved into 6.6 L of ACN. The basic deprotection was carried out by DEA.
The reaction mixture was diluted with iPrOAc and the peptide rich organic solution was washed with an aqueous solution of KHSO4 and NaCl, then concentrated and the residue dissolved into iPrOAc.
The solution was precipitated by addition of DIPE, filtered, washed and dried. H-Ala-Cys[Ψ(Dmp,H)pro]-Thr(tBu)-Gly-Cys[Ψ(Dmp,H)pro]-Tyr(tBu)-OtBu was obtained with Purity HPLC 90.3% and Yield 91%
1087 grams of H-Ala-Cys[Ψ(Dmp,H)pro]-Thr(tBu)-Gly-Cys[Ψ(Dmp,H)pro]-Tyr(tBu)-OtBu and 698 grams of Fragment B were dissolved into 5.4 L of ACN and the coupling reaction carried out by Oxyma-EDC*HCl/NMM system.
The reaction mixture was precipitated by the addition of an aqueous solution of NaHCO3, filtered, washed and dried.
Fmoc-Asn(Trt)-Pro-Ala-Cys[Ψ(Dmp,H)pro]-Thr(tBu)-Gly-Cys[Ψ(Dmp,H)pro]-Tyr(tBu)-OtBu was obtained with Purity HPLC 87.3% and Yield 98%
1727 grams of Fmoc-Asn(Trt)-Pro-Ala-Cys[Ψ(Dmp,H)pro]-Thr(tBu)-Gly-Cys[Ψ(Dmp,H)pro]-Tyr(tBu)-OtBu was dissolved into 8.6 L of iPrOAc. The basic deprotection was carried out by DEA.
The reaction mixture was diluted with iPrOAc and the peptide rich organic solution was washed with an aqueous solution of KHSO4 and NaCl, then concentrated, and the residue dissolved into iPrOAc.
The solution was precipitated by addition of DIPE, filtered, washed.
The product was then dried.
H-Asn(Trt)-Pro-Ala-Cys[Ψ(Dmp,H)pro]-Thr(tBu)-Gly-Cys[Ψ(Dmp,H)pro]-Tyr(tBu)-OtBu was obtained with Purity HPLC 89.0% and Yield 89%
1339 grams of H-Asn(Trt)-Pro-Ala-Cys[Ψ(Dmp,H)pro]-Thr(tBu)-Gly-Cys[Ψ(Dmp,H)pro]-Tyr(tBu)-OtBu and 512 grams of Fmoc-Cys(Trt)-OH were dissolved into 6.7 L of DMF and the coupling reaction was carried out by TOTU/NMM system.
The reaction mixture was precipitated by an aqueous solution of NaHCO3, filtered, washed dried.
Fmoc-Cys(Trt)-Asn(Trt)-Pro-Ala-Cys[Ψ(Dmp,H)pro]-Thr(tBu)-Gly-Cys[Ψ(Dmp,H)pro]-Tyr(tBu)-OtBu was obtained with Purity HPLC 84.3% and Yield 95%
1733 grams of Fmoc-Cys(Trt)-Asn(Trt)-Pro-Ala-Cys[Ψ(Dmp,H)pro]-Thr(tBu)-Gly-Cys[Ψ(Dmp,H)pro]-Tyr(tBu)-OtBu were dissolved into 8.7 L of iPrOAc. The basic deprotection was carried out by DEA.
The reaction mixture was diluted with iPrOAc and the peptide rich organic solution was washed firstly with an aqueous solution of KHSO4, NaHCO3 and NaCl, then concentrated, and the obtained residue was dissolved into DMF (8.7 L).
Fmoc-Cys(SO3Na)-ONa (404 grams) was added and the coupling reaction carried out by PyOxim/2,4,6 collidine system.
The reaction mixture was precipitated by an aqueous solution of NaHCO3, filtered, washed and dried.
The dried product was dissolved in 8.7 L of iPrOAc. The basic deprotection was carried out by DEA.
The reaction mixture was diluted with iPrOAc and the peptide rich organic solution was washed with an aqueous solution of KHSO4 and NaCl, then concentrated, and the obtained residue was dissolved into iPrOAc.
The obtained solution was precipitated by addition of DIPE, filtered and washed.
The product was then dried.
H-Cys(SO3Na)-Cys(Trt)-Asn(Trt)-Pro-Ala-Cys[Ψ(Dmp,H)pro]-Thr(tBu)-Gly-Cys[Ψ(Dmp,H)pro]-Tyr(tBu)-OtBu was obtained with Purity HPLC 82.9% and Yield 83%
Fmoc-Cys(SO3Na)-Cys(SO3Na)-Glu(tBu)-Tyr(tBu)-Cys(SO3Na)-Cys(Trt)-Asn(Trt)-Pro-Ala-Cys[Ψ(Dmp,H)pro]-Thr(tBu)-Gly-Cys[Ψ(Dmp,H)pro]-Tyr(tBu)-OtBu
1400 grams of H-Cys(SO3Na)-Cys(Trt)-Asn(Trt)-Pro-Ala-Cys[Ψ(Dmp,H)pro]-Thr(tBu)-Gly-Cys[Ψ(Dmp,H)pro]-Tyr(tBu)-OtBu and 850 grams of Fragment A were dissolved into 7.0 L of ACN and the coupling reaction carried out by Oxyma/B-EDC*HCl/2,4,6 collidine system.
The reaction mixture was precipitated by an aqueous solution of NaHCO3, filtered, washed and dried.
Linear protected peptide was obtained with Purity HPLC 66.7% and quantitative Yield.
STEP I—Synthesis of H-Cys(SO3Na)-Cys(SO3Na)-Glu(tBu)-Tyr(tBu)-Cys(SO3Na)-Cys(Trt)-Asn(Trt)-Pro-Ala-Cys[Ψ(Dmp,H)pro]-Thr(tBu)-Gly-Cys[Ψ(Dmp,H)pro]-Tyr(tBu)-OtBu
2126 grams of linear protected peptide was dissolved into 10.6 L of ACN. The basic deprotection was carried out by DEA.
The reaction mixture was diluted with iPrOAc and the peptide rich organic solution was washed with an aqueous solution of KHSO4 and NaCl, then concentrated, and the obtained residue was dissolved into iPrOAc.
The solution was precipitated by addition of n-heptane, filtered, washed and dried.
H-Cys(SO3Na)-Cys(SO3Na)-Glu(tBu)-Tyr(tBu)-Cys(SO3Na)-Cys(Trt)-Asn(Trt)-Pro-Ala-Cys[Ψ(Dmp,H)pro]-Thr(tBu)-Gly-Cys[Ψ(Dmp,H)pro]-Tyr(tBu)-OtBu was obtained with Purity HPLC 61.7% and Yield 89%
H-Cys(SO3H)-Cys(SO3H)-Glu-Tyr-Cys(SO3H)-Cys-Asn-Pro-Ala-Cys-Thr-Gly-Cys-Tyr-OH TFA salt
450 grams of H-Cys(SO3Na)-Cys(SO3Na)-Glu(tBu)-Tyr(tBu)-Cys(SO3Na)-Cys(Trt)-Asn(Trt)-Pro-Ala-Cys[Ψ(Dmp,H)pro]-Thr(tBu)-Gly-Cys[Ψ(Dmp,H)pro]-Tyr(tBu)-OtBu were treated with a solution of TFA-water-TIS (20.3 L-2.3 L-0.5 L) The reaction mixture was then precipitated by addition of MTBE filtered, washed and dried.
Linear advanced intermediate was obtained with Purity HPLC 57.9% and Yield 92%.
90 grams of linear advanced intermediate (estimated 49 grams of peptide 100%) was charged in a mix phosphate buffer pH=7.4 and IPA and the reaction mixture was kept stirring for 16-32 hours.
The mixture was concentrated to remove IPA and then filtered.
Crude Linaclotide in solution (30 grams peptide content) was obtained with Purity HPLC 53.8% and cyclization Yield 72%.
Crude Linaclotide in solution (75.4 grams Linaclotide content) was loaded onto C18 column (100 mm diameter×350 mm max height) and purified by applying 3 different and consecutive chromatographic steps
Gradient: from 0% to 100% of Eluent B in 180 minutes
Eluent A: 0.15% TFA
Eluent B: Eluent A-ACN 1-1
Gradient: from 0% to 100% of Eluent B in 180 minutes
Eluent A: 20 mM NH4H2PO4 buffer pH 6
Eluent B: Eluent A-ACN 1-1
Gradient: from 0% to 100% of Eluent B in 180 minutes
Eluent A: 20-100 mM AcOH
Eluent B: Eluent A-ACN 1-1
Finally, 41.7 grams of Linaclotide purified in solution were obtained starting from 75.4 grams; chromatographic purity 99.97% and Yield (3 steps) 55%.
Linaclotide purified solution (41.7 grams, Linaclotide concentration 4.2 g/L) was processed as follows:
to obtain 46.4 grams of amorphous High-Purity Linaclotide powder corresponding to 40.9 grams Linaclotide 100% (Assay 88.1%).
The obtained product has the following analytical attributes:
The obtained product was submitted to PXRD and DSC analyses which confirm that the powder is totally amorphous (
Linaclotide purified solution (42.9 grams Linaclotide content, concentration 4.3 g/L) was processed as follows:
to obtain 40.9 grams of amorphous High-Purity Linaclotide powder corresponding to 39.1 grams Linaclotide 100% (Assay 95.7%).
The obtained product has the following analytical attributes:
The obtained product was submitted to PXRD and DSC analysis and the powder was confirmed to be a totally amorphous solid (
| Number | Date | Country | Kind |
|---|---|---|---|
| 21214095.8 | Dec 2021 | EP | regional |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/EP2022/085451 | 12/12/2022 | WO |
