Claims
- 1. A purified polynucleotide molecule, comprising a nucleotide sequence that encodes an oxalyl-CoA decarboxylase polypeptide, or a fragment of said oxalyl-CoA decarboxylase that retains functional enzymatic activity.
- 2. The polynucleotide molecule according to claim 1, wherein said nucleotide sequence is derived from Oxalobacter formigenes.
- 3. The polynucleotide molecule according to claim 1, wherein said nucleotide sequence encodes a polypeptide comprising the amino acid sequence shown in SEQ ID NO. 4, or a fragment of said oxalyl-CoA decarboxylase that retains functional enzymatic activity.
- 4. The polynucleotide molecule according to claim 1, comprising the nucleotide sequence shown in SEQ ID NO. 3.
- 5. The polynucleotide molecule according to claim 1, wherein said polynucleotide molecule hybridizes under standard high-stringency conditions with a polynucleotide molecule comprising the nucleotide sequence shown in SEQ ID NO. 3, or the complementary sequence thereof.
- 6. The polynucleotide molecule according to claim 1, wherein said polynucleotide consists of a nucleotide sequence that encodes oxalyl-CoA decarboxylase comprising the amino acid sequence shown in SEQ ID NO. 4, or a fragment of said oxalyl-CoA decarboxylase that retains functional enzymatic activity.
- 7. The polynucleotide molecule according to claim 6, wherein said polynucleotide hybridizes under high stringency conditions with a nucleotide sequence comprising nucleotides 181 through 1884 of the nucleotide sequence shown in SEQ ID NO. 3 or the complementary sequence thereof.
- 8. The polynucleotide according to claim 6, wherein said nucleotide sequence consists of nucleotides 181 through 1884 of the nucleotide sequence shown in SEQ ID NO. 3 or the complementary sequence thereof.
- 9. A polynucleotide probe, comprising a nucleotide sequence that is substantially complementary with a polynucleotide sequence present in an Oxalobacter formigenes genome, wherein the polynucleotide sequence present in the Oxalobacter formigenes genome comprises a gene selected from the group consisting of the formyl-CoA transferase gene and the oxalyl-CoA decarboxylase gene.
- 10. A polynucleotide PCR primer, comprising a nucleotide sequence that is substantially complementary with a polynucleotide sequence present in an Oxalobacter formigenes genome, wherein said polynucleotide sequence present in said Oxalobacter formigenes genome comprises a gene selected from the group consisting of the formyl-CoA transferase gene and the oxalyl-CoA decarboxylase gene, and wherein said PCR primer is capable of priming PCR amplification of said polynucleotide sequence present in said Oxalobacter formigenes genome.
- 11. A method for detecting Oxalobacter formigenes in a sample, comprising the steps of:
(a) contacting said sample with a polynucleotide probe according to claim 9 under conditions sufficient for selective hybridization of said polynucleotide probe with a DNA fragment specific for Oxalobacter formigenes; and (b) detecting said probe hybridized to said DNA fragment.
- 12. A polynucleotide vector comprising a polynucleotide molecule according to claim 1.
- 13. The polynucleotide vector according to claim 12, wherein said polynucleotide molecule consists of a nucleotide sequence that encodes an oxalyl-CoA decarboxylase polypeptide comprising the amino acid sequence shown in SEQ ID NO. 4, or a fragment of said oxalyl-CoA decarboxylase that retains functional enzymatic activity.
- 14. The polynucleotide vector according to claim 12, wherein said polynucleotide vector hybridizes under high stringency conditions with a nucleotide sequence consisting of nucleotides 181 through 1884 of the nucleotide sequence shown in SEQ ID NO. 3 or the complementary sequence thereof.
- 15. The polynucleotide vector according to claim 12, wherein said nucleotide sequence of said polynucleotide molecule consists of nucleotides 181 through 1884 of the nucleotide sequence shown in SEQ ID NO. 3 or the complementary sequence thereof.
- 16. A recombinant host cell which comprises the polynucleotide vector of claim 12, and wherein said cell expresses said oxalyl-CoA decarboxylase or a fragment of said oxalyl-CoA decarboxylase that retains enzymatic activity.
- 17. The cell according to claim 16, wherein said polynucleotide vector hybridizes under high stringency conditions with a nucleotide sequence consisting of nucleotides 181 through 1884 of the nucleotide sequence shown in SEQ ID NO. 3 or the complementary sequence thereof.
- 18. The cell according to claim 16, wherein said nucleotide sequence of said polynucleotide molecule consists of nucleotides 181 through 1884 of the nucleotide sequence shown in SEQ ID NO. 3 or the complementary sequence thereof.
Parent Case Info
[0001] Cross-Reference to a Related Applications
[0002] This application is a continuation of U.S. application Ser. No. 09/829,094, filed Apr. 9, 2001, which is a continuation of U.S. application Ser. No. 08/936,094, filed Sep. 23, 1997, now U.S. Pat. No. 6,214,980; which is a continuation-in-part of patent application Ser. No. 08/883,610, filed Jun. 26, 1997, now U.S. Pat. No. 6,090,628; which is a continuation-in-part of patent application Ser. No. 08/717,587, filed Sep. 27, 1996, now U.S. Pat. No. 5,912,125; which is a continuation-in-part of patent application Ser. No. 08/493,197, filed Jun. 20, 1995, now U.S. Pat. No. 5,837,833; which is a continuation-in-part of patent application Ser. No. 08/262,424, filed Jun. 20, 1994, now U.S. Pat. No. 5,604,111.
Government Interests
[0003] This invention was made with government support under National Institutes of Health Grant No. DK 20586. The government has certain rights in this invention.
Continuations (2)
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Number |
Date |
Country |
Parent |
09829094 |
Apr 2001 |
US |
Child |
10658564 |
Sep 2003 |
US |
Parent |
08936094 |
Sep 1997 |
US |
Child |
09829094 |
Apr 2001 |
US |
Continuation in Parts (4)
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Number |
Date |
Country |
Parent |
08883610 |
Jun 1997 |
US |
Child |
08936094 |
Sep 1997 |
US |
Parent |
08717587 |
Sep 1996 |
US |
Child |
08883610 |
Jun 1997 |
US |
Parent |
08493197 |
Jun 1995 |
US |
Child |
08717587 |
Sep 1996 |
US |
Parent |
08262424 |
Jun 1994 |
US |
Child |
08493197 |
Jun 1995 |
US |