Patent Application
20220373558
The application relates to methods and reagents for, inter alia, characterizing molecular conjugates, including, for example quantifying, normalizing, detecting and/or identifying conjugated molecules. As taught in the application, the present invention is useful for use with various types of molecules, including, for example, molecular conjugates, drug conjugates, such as antibody-drug conjugates (ADCs), and the like.
Conjugation of molecules can be advantageous. For example, drug conjugates, such as antibody-drug conjugates (ADCs), are a growing class of therapies for treating cancers (Polakis, Pharmacological reviews 68, 3-19 (2016)) and immunological disorders (McPherson. Methods in molecular biology 2078, 23-36 (2020)). A general structure of an ADC contains a monoclonal antibody (mAb) connected to a drug via a cleavable or a non-cleavable linker. For example, the ADC can have a humanized/human mAb connected to biologically active payloads, such as cytotoxics, steroids and anti-sense oligonucleotides, via a non-cleavable or cleavable linker, such as an acid labile linker, protease cleavable linker, or disulfide linker. The linker can be covalently linked to the mAb at a conjugation site via lysine coupling, cysteine alkylation or an enzymatic reaction. The ADC binds to its target cell-surface antigen receptor, which enables targeted delivery of the drug to a target site and minimizes systemic toxicity effects to healthy tissue, resulting in increased selectivity, improved efficacy and safety over alternative chemotherapeutic methods or other first generation mAbs (see, e.g., Dan et al., 2018, Pharmaceuticals (Basel) 11).
Perfected conjugation is difficult to achieve. Incomplete conjugation processes can result in free or non-conjugated drug, drug-linker, or drug related impurities. Degradation products can occur over time in formulations as well as in vivo. Characterization and quantification of ADCs are important for the safety, efficacy, and uniformity of the ADCs.
The present application provides improved, such as more accurate, reliable, sensitive, etc., materials and methods for efficient identification and quantification of molecular conjugates, including therapeutic conjugates, such as drug conjugates. The present invention teaches materials and methods for, for example, such improved characterization.
The present application relates to, inter alia, a method of analyzing a conjugate comprising a drug covalently linked to a polypeptide by using Tandem Mass Tag (TMT).
In one aspect, the application relates to a method of analyzing a conjugate comprising a drug covalently linked to a polypeptide, comprising:
In another aspect, a sample comprising a conjugate of a drug covalently linked to a polypeptide is analyzed together with a control sample comprising the polypeptide not covalently linked to the drug. The method comprises:
In one aspect, a method of the application is used to determine the occupancy ratio of a site of conjugation in a conjugate. For example, the individual site occupancy of a conjugate can be determined by: 1) obtaining a mass spectrum for a peptide labeled with both of the first TMT and the second TMT and a peptide labeled with both of the third TMT and the fourth TMT using a method of the application, wherein the mass spectrum comprises a reporter ion of the first TMT, a reporter ion of the third TMT, a reporter ion of the second TMT and a reporter ion of the fourth TMT; 2) detecting the mass-to-charge ratio (m/z) associated with the reporter ions in the mass spectrum; and 3) determining the occupancy ratio of a site of conjugation in the conjugate based on the intensity of the reporter ion of the first TMT and the intensity of the reporter ion of the third TMT, or the intensity of the reporter ion of the second TMT and the intensity of the reporter ion of the fourth TMT in the mass spectrum, preferably the occupancy ratio is determined by the following equation:
(the intensity of the reporter ion of the third TMT−the intensity of the reporter ion of the first TMT)/the intensity of the reporter ion of the third TMT, or
(the intensity of the reporter ion of the fourth TMT−the intensity of the reporter ion of the second TMT)/the intensity of the reporter ion of the fourth TMT.
In certain embodiments, the occupancy ratio of the site of conjugation in the conjugate is determined at various time points, wherein additional TMTs are used to label the polypeptides at different time points.
In another embodiment, a method of the application is used to normalize a conjugate sample with a control sample. For example, a conjugate sample is normalized with a control sample by a method comprising: 1) obtaining a mass spectrum for a peptide labeled with only the second TMT and a peptide labeled with only the fourth TMT using the method of claim 2, wherein the mass spectrum comprises a reporter ion of the second TMT and a reporter ion of the fourth TMT, but not a reporter ion of the first TMT or third TMT; 2) detecting the mass-to-charge ratio (m/z) associated with the reporter ions; and 3) normalizing the sample with the control sample by the ratio of the intensity of the reporter ion of the second TMT to that of the reporter ion of the fourth TMT.
In yet another embodiment, a method of the application is used to localize the drug conjugation site, e.g., the site where the drug covalently binds to the polypeptide, in a conjugate. For example, the drug conjugation site can be localized by a method comprising: 1) obtaining a mass spectrum of a peptide labeled only with the second TMT using a method of the application, wherein the mass spectrum comprises only a reporter ion of the second TMT, but not a reporter ion of the first, third or fourth TMT; and 2) triggering a second tandem mass spectrometry analysis on the peptide labeled only with the reporter ion of the second TMT to thereby localize the drug conjugation site. In certain embodiments, the peptide labeled only with the reporter ion of the second TMT, which is obtained after the digestion of the second mixture, is completely conjugated to the drug, e.g., all amino acid residues on the peptide that are capable of conjugating to the drug have been covalently linked to the drug. The peptide can be conjugated to only one drug, when the peptide contains only one amino acid residue (e.g., Cys or Lys) capable of forming a covalent bond with the drug. The peptide can also be conjugated to more than one drugs, when the peptide contains more than one amino acid residues (e.g., Cys or Lys) capable of forming a covalent bond with the drug.
In certain embodiment, a method of localizing the drug conjugation site in the conjugate, comprises: 1) obtaining a mass spectrum of a peptide labeled only with the first and the second TMTs using a method of the application, wherein the mass spectrum comprises only reporter ions of the first and the second TMTs, but not a reporter ion of the third or fourth TMT; and 2) triggering a second tandem mass spectrometry analysis on the peptide labeled only with the first and the second TMTs to thereby localize the drug conjugation site. In certain embodiments, the peptide labeled only with the first and second TMTs, which is obtained after the digestion of the second mixture, is incompletely conjugated to the drug, e.g., only some, but not all, amino acid residues capable of conjugating to the drug have been covalently linked to the drug.
In one embodiment of the application, the conjugate is a drug antibody conjugate (ADC), more preferably, the ADC comprises a drug covalently linked to one or more cysteine (Cys) or lysine (Lys) residues of a monoclonal antibody.
As a person of ordinary skill in the art would appreciate, various suitable tandem mass spectrometry can be used in a method of the application in view of the present disclosure. See, e.g., Friese et al., MAbs 10, 335-345 (2018), for a review of tandem mass spectrometry, the content of which is incorporated herein by reference in its entirety. In certain embodiments, a high energy collision-induced dissociation tandem mass spectrometry (HCD-MS2) is used to obtain the mass spectrum of a peptide comprising at least one reporter ion of the first TMT, the second TMT, the third TMT and the fourth TMT. In other embodiments, after one or more TMT reporter ions are detected from a peptide from the HCD-MS2 analysis, additional dissociation analysis is triggered for additional characterization of the peptide, e.g., to localize the drug conjugation site in the peptide. The additional dissociation can be conducted by a second tandem mass spectrometry analysis. Examples of second tandem mass spectrometry useful for such a method include, but are not limited to, an electron transfer dissociation tandem mass spectrometry (ETD-MS2) or an electron-capture dissociation tandem mass spectrometry (ECD-MS2).
In other embodiments, a higher trigger intensity threshold and/or a narrow isolation window is used to improve the triggering of the second tandem mass spectrometry. In certain embodiments, synchronous precursor selection with tribrid technology is applied to the tandem mass spectrometry to improve the specificity and accuracy of the detection and quantification.
Any suitable TMTs can be used in view of the disclosure in the application. See, e.g., Bachor et al., Molecules 2019, 24, 701, for a review of TMTs, the content of which is incorporated herein by reference in its entirety. In some embodiments of the application, the first isobaric set of TMTs comprises two or more TMTs reactive to reduced cysteine. Preferably, the first isobaric set of TMTs comprises two, three, four, five, six or more TMTs reactive to reduced cysteine. In some embodiments, the first isobaric set comprises two, three, four, five, six or more isobaric isomers (e.g., same mass and structure) that are iodoacetyl-activated for covalent, irreversible labeling of sulfhydryl (—SH) groups. In some embodiment, the first isobaric set comprises two, three, four, five or six isobaric isomers of the IodoTMTsixplex isobaric label reagent set, which is available from ThermoFisher Scientific (Catalog No. 90101).
In certain embodiments, each of the first TMT and the third TMT comprises a mass reporter, a mass normalizer and a cysteine reactive group that are covalently linked to each other. Thus, each of the first and third TMTs labels reduced cysteine in the polypeptide of the conjugate, and can be used for the analysis of a conjugate containing a drug covalently linked to one or more cysteine residues of the polypeptide. Preferably, each of the first and third TMT is selected from the isobaric set of IodoTMTsixplex.
In some embodiments of the application, the second isobaric set of TMTs comprises two, three, four, five, six or more TMTs reactive with lysine or the primary amine at the N-terminus of a peptide. In one embodiment of the application, the second isobaric set of TMTs comprises two, three, four, five, six, seven, eight, nine, ten or more isobaric compounds having an amine-reactive NHS-ester group, a spacer arm and a mass reporter. For example, the second isobaric set can comprise two, three, four, five, six or more isobaric isomers of the TMT10plex™ (ThermoFisher, Catalog No. 90110), the TMTsixplex™ (ThermoFisher, Catalog No. 90061), or the TMTpro™ 16 plex (ThermoFisher, Catalog No. A44520) label reagent sets.
In certain embodiments, each of the second TMT and the fourth TMT comprises a mass reporter, a mass normalizer and an amine reactive group that are covalently linked to each other. Thus, each of the second and fourth TMTs labels lysine or the N-terminus of the polypeptide of the conjugate, and can be used together with the first and third TMTs for the analysis of a conjugate containing a drug covalently linked to one or more cysteine residues of the polypeptide. Preferably, each of the second TMT and the fourth TMT is selected from an isobaric set of TMT6plex, TMT10plex or TMT pro16 plex.
In some embodiments, the first isobaric set and the second isobaric set of TMTs each independently comprise two, three, four, five, six or more TMTs reactive with lysine or the primary amine at the N-terminus of a peptide. In certain embodiments, each of the first, second, third and fourth TMTs comprises a mass reporter, a mass normalizer and an amine reactive group that are covalently linked to each other. The TMTs can be used for the analysis of a conjugate containing a drug covalently linked to one or more lysine residues of the polypeptide.
In some embodiments, a multiplex of samples comprising one or more conjugates are analyzed together. In certain embodiments, the drug conjugation site in the conjugate is characterized by a mass spec bar code comprising (2n+2) reporter ions, and the conjugate is normalized by a mass spec bar code comprising n+1 reporter ions, and n is the number of samples analyzed by the method.
Other aspects of the application relate to a system for conducting any of the method of claims 1-23.
Yes another aspect of the application relates to a composition comprising a mixture of peptides labeled with at least one of a first TMT and a second TMT, optionally one or more unlabeled peptides, wherein the first TMT and the second TMT do not have the same reporter ion mass, and the mixture of peptides comprises at least one peptide that is conjugated to a drug and labeled with the at least one of the first TMT and the second TMT.
Other aspects, features and advantages of the invention will be apparent from the following disclosure, including the detailed description of the invention and its preferred embodiments and the appended claims, which are exemplary and nonlimiting, as would be appreciated by a person having ordinary skill in the art.
The foregoing summary, as well as the following detailed description of preferred embodiments of the present application, will be better understood when read in conjunction with the appended drawings. It should be understood, however, that the application is not limited to the precise embodiments shown in the drawings.
The patent or application file contains at least one drawing executed in color. Copies of this patent or patent application publication with color drawing(s) will be provided by the Office upon request and payment of the necessary fee.
Unless indicated otherwise, in all the drawings herein, the reporter ion of IdoTMT is represented by the reporter ion of TMT6 is represented by
the analyzed peptide is represented by
and the drug is represented by
.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as is commonly understood by one of ordinary skill in the art to which this invention pertains. Otherwise, certain terms used herein have the meanings as set in the specification. All patents, published patent applications and publications cited herein are incorporated by reference as if set forth fully herein.
Discussion of documents, acts, materials, devices, articles or the like which has been included in the present specification is for the purpose of providing context for the present invention. Such discussion is not an admission that any or all of these matters form part of the prior art with respect to any inventions disclosed or claimed.
It must be noted that as used herein and in the appended claims, the singular forms “a,” “an,” and “the” include plural reference unless the context clearly dictates otherwise.
Unless otherwise stated, any numerical value, such as a concentration or a concentration range described herein, are to be understood as being modified in all instances by the term “about.” Thus, a numerical value typically includes ±10% of the recited value. For example, an amount of about 50 ppm or less includes 45 ppm or less to 55 ppm or less. As used herein, the use of a numerical range expressly includes all possible subranges, all individual numerical values within that range, including integers within such ranges and fractions of the values unless the context clearly indicates otherwise.
Throughout this specification and the claims which follow, unless the context requires otherwise, the word “comprise,” and variations such as “comprises” and “comprising,” will be understood to imply the inclusion of a stated integer or step or group of integers or steps but not the exclusion of any other integer or step or group of integer or step. When used herein the term “comprising” can be substituted with the term “containing” or “including” or sometimes when used herein with the term “having.”
When used herein “consisting of” excludes any element, step, or ingredient not specified in the claim element. When used herein, “consisting essentially of” does not exclude materials or steps that do not materially affect the basic and novel characteristics of the claim. Any of the aforementioned terms of “comprising”, “containing”, “including”, and “having”, whenever used herein in the context of an aspect or embodiment of the application can be replaced with the term “consisting of” or “consisting essentially of” to vary scopes of the disclosure.
As used herein, the conjunctive term “and/or” between multiple recited elements is understood as encompassing both individual and combined options. For instance, where two elements are conjoined by “and/or,” a first option refers to the applicability of the first element without the second. A second option refers to the applicability of the second element without the first. A third option refers to the applicability of the first and second elements together. Any one of these options is understood to fall within the meaning, and therefore satisfy the requirement of the term “and/or” as used herein. Concurrent applicability of more than one of the options is also understood to fall within the meaning, and therefore satisfy the requirement of the term “and/or.”
As used herein, “MS/MS” or “MS2” or “MS2” refers to tandem mass spectrometry. Tandem mass spectrometry is a technique in instrumental analysis where two or more mass analyzers are coupled together using an additional reaction step to increase their abilities to analyze samples. Tandem use of mass analysis can be done where the reaction steps are separated in space (tandem in space) and/or reaction steps are separated in time (tandem in time). A common use of tandem mass spectrometry is the analysis of biomolecules, such as proteins, peptides, organic and inorganic molecules, lipid, metabolites, and oligonucleotides.
As used herein, “reporter ion” or “diagnostic ion” refers to a characteristic product ion of a labeled peptide containing an N-terminal tag or label, which is observed in the ETD mass spectrum. Usually, it is the most dominant product ion in the mass spectrum, and it is used to trigger subsequent MS/MS events to further sequence the labeled peptide.
As used herein, “tribrid technology” refers to a technology that uses hybrid mass spectrometers having more than a single type of mass analyzer. It enables the performance of tandem mass spectrometry experiments with great flexibility for TMT sample multiplexing. The tribrid technology can be used for characterization of challenging samples including low-abundance peptides in complex matrices, determination of positional and posttranslational isoforms of intact proteins, resolution of isobaric metabolites, and protein structure characterization using chemical crosslinking.
As used herein, “orbitrap” or “OT” refers to an ion trap mass analyzer that consists of two outer electrodes and a central electrode. This setup enables the orbitrap to function as both an analyzer and detector. Ions that enter the orbitrap are captured and oscillate around the central electrode and in between the two outer electrodes. Different ions oscillate at different frequencies, which results in their separation. The oscillation frequencies that induced by ions on the outer electrodes are measured and the mass spectra of the ions are acquired using image current detection.
As used herein, “isobaric” refers to having the same nominal molecular weight or formula weight. Preferably, isobaric TMTs useful for an invention of the application have the same mass and structure, and they are also called isotopomers.
As used herein, “ion” refers to a molecule that has a net electric charge due to the loss or gain of one or more electrons. Examples of an ion can be a, b, or y-type product ions that are the result of cleaved amide bonds along the backbone of a protein due to collision-induced dissociation (CID).
As used herein, “sequence ion” refers to an ion (or ions) that correspond to the product of a particular peptide that is split at a given peptide bond.
As used herein, “immonium ion” refers to an ion (or ions) that correspond to an internal fragment of a peptide that has a single side chain formed by a combination of -type or y-type cleavage.
As used herein, “reporter ion” refers to an ion that is cleaved from an isobaric tagged peptide by methods known in the art (e.g., MS2).
As used herein, “isotopologue” refers to a molecule that differs from its parent molecule in that at least one atom has a different number of neutrons.
As used herein, “multipole” refers to an ion guide that is comprised of metal rods to transport ions through a vacuum system.
As used herein, “conjugate” refers to a protein or peptide covalently linked to one or more heterologous molecule(s). Examples of the protein or peptide that can be covalently linked to the heterologous molecule(s) include, but are not limited to, a therapeutic peptide or protein, an antibody or a fragment thereof. Examples of the heterologous molecule(s) that can be covalently linked to the protein or peptide include, but are not limited to, one or more small molecule compounds, a label, etc.
As used herein, “peptide” refers to an amino acid based polymer usually composed of some combination of the twenty common naturally-occurring amino acids, but can also contain or be completely composed of unnatural amino-acid monomer residues. It can include a linear amino acid polymer configuration, or can include a cyclic peptide, or a branched one, or any combination of all three configurations. A peptide can also have any combination of naturally occurring modifications (e.g., phosphorylation or glycosylation) or unnaturally occurring modifications (e.g., carbamidomethylation).
“Antibody” shall include, without limitation, (a) an immunoglobulin molecule comprising two heavy chains and two light chains and which recognizes an antigen; (b) a polyclonal or monoclonal immunoglobulin molecule; and (c) a monovalent or divalent fragment thereof. Immunoglobulin molecules can derive from any of the commonly known classes, including but not limited to IgA, secretory IgA, IgG, IgE and IgM. IgG subclasses are well known to those in the art and include, but are not limited to, human IgG1, IgG2, IgG3 and IgG4. Antibodies can be both naturally occurring and non-naturally occurring. Furthermore, antibodies include multi-specific antibodies, such as bi-specific antibodies, tri-specific antibodies, tetra-specific antibodies, wholly synthetic antibodies, single chain antibodies, and fragments thereof. Antibodies can be human or nonhuman. Antibody fragments include, without limitation, Fab fragments, Fv fragments and other antigen-binding fragments.
As used herein, a “tandem mass tag” or “TMT” refers a chemical label that can be used for mass spectrometry (MS)-based quantification and identification of a molecule. Any molecule that has free thiols or primary amines or glycans can be tagged. For example, the molecule can be a protein or peptide. The TMT tags typically contain at least three regions, e.g., a mass reporter region, a mass normalization or balance region, and a reactive group. The reporter mass can be incremented to create unique masses while the balance mass is used to-offset the total mass of the reagent to make a series of reagents that have the same overall mass yet having a distinct reporter mass. The reactive group facilitates the addition reaction of amines, thiols, oxonium or other functional groups. It can be, for example, free thiol or reduced cysteine reactive, primary amine or lysine reactive, or aminoxy reactive. Optionally, the TMT can also contain one or more cleavable linker regions.
An isobaric set of TMTs enable concurrent identification and multiplexed quantitation of proteins in different samples using tandem mass spectrometry. An isobaric set of n TMTs can contain a TMT and TMTs with n−1 isotopic substitutions. The chemical structures of all the tags within an isobaric set of TMTs are identical, but each contains isotopes substituted at various positions, such that the mass reporter and mass normalization regions have different molecular masses in each tag, while the TMTs have the same total molecular weights (isobaric) and structure so that during chromatographic or electrophoretic separation and in single MS mode, molecules labelled with different tags are indistinguishable. For example, each isobaric reagent can contain a different number of heavy isotopes in the mass reporter region, which results in a unique reporter mass during tandem MS/MS for sample identification and relative quantitation. Upon fragmentation in MS/MS mode, sequence information is obtained from fragmentation of the peptide back bone and quantification data are simultaneously obtained from fragmentation of the tags, giving rise to mass reporter ions. Each TMT within an isobaric set generate a unique reporter mass on the MS/MS spectrum.
TMT labels, such as those commercially available TMT labels, can multiplex samples (such as 6-plex, 10-plex or more) and are reactive toward cysteine or amines, respectively. Shifting the location of the heavy isotopes between the reporter group and the spacer, the total mass and chemical structure of each tag can be kept the same (isobaric). For example, the cysteine reactive IodoTMTsixplex reagents and amine reactive TMTsixplex reagents each have six identical reporter ion masses. However, each reporter ion is unique in mass to charge ratio (m/z), or the reporter mass, also called reporter ion mass. For example, the nominal reporter masses of 6 TMTs within the isobaric TMT6 set range from 126-131 Da (Dalton).
An isobaric label reagent set of “IodoTMTsixplex”, also called “CysTMT”, “IodoTMT”, “IodoTMT6” or “IodoTMT6”, refers to an isobaric set of six TMTs that are iodoacetyl-activated for covalent, irreversible labeling of sulfhydryl (—SH) groups, and have the chemical structures shown in
An isobaric label reagent set of “TMTsixplex”, also referred to herein as “TMT6”, “TMT6”, “TMT6 plex” refers to an isobaric set of six TMTs that are NHS-activated for covalent, irreversible labeling of primary amines (—NH2) groups and have the chemical structures shown in
An isobaric label reagent set of “TMT10plex”, also referred to herein as “TMT10”, “TMT10”, or “TMT10 plex”, refers to an isobaric set of ten TMTs each having an amine-reactive NHS-ester group, a spacer arm and a mass reporter, and having the chemical structures shown in
An isobaric label reagent set of “TMTpro 16plex”, also referred to herein as “TMT16”, “TMT16”, “TMTpro” or “TMTpro16 plex”, refers to an isobaric set of 16 amine-reactive NHS ester-activated reagents having the chemical structures shown in
A set of 6 reporters (TMT-6 plex), set of 10 reporters (TMT-10 plex), set of 11 reporters (TMT-11 plex) and up to 16 reporters (TMT-16 plex) of such TMTs can be used in methods of the invention. Other examples of TMTs can also be used in the invention in view of the present disclosure. In particular, TMT16-plex and future n-plex reagents where n>16 would increase the number of samples that can be labeled with dual TMT labeling. The TMTs can be made using methods known in the art or obtained from commercial sources such as ThermoFisher Scientific. When two TMTs are used in tandem, however, they cannot have the same reporter ion mass. For example, because IodoTMT and TMT6 have the same series of reporter masses, when the isobaric sets of IodoTMT and TMT6 are used for dual labeling, the selected IodoTMT(s) and TMTs must not have the same reporter mass when they are used for dual labeling. Similarly, when more than two TMTs are used in the same analysis, such as the TMTs used for the analysis of the sample and mock together or for the analysis of multiple samples together, each of the TMTs used in the analysis must have unique reporter mass, or none of the TMTs used in the same assay can have the same reporter mass.
An important metric for ADC efficiency, safety, and selectivity is determined by drug-antibody ratios (DARs). Chromatographic approaches, e.g., size exclusion chromatography (SEC), hydrophobic interaction chromatography (HIC), and reversed phase liquid chromatography (RPLC), can possibly be used to obtain the DAR value and characterize ADCs. However, these approaches have significant disadvantages, for example they, for example, they usually exhibit low throughput due to the long separation and column regeneration times. Moreover, certain required mobile phases prevent online coupling to mass spectrometry (MS). Native MS-based approaches preserve non-covalent interactions and consequently can obtain information on the array of possible drug conjugate species.
Another approach is ion mobility spectrometry coupled with mass spectrometry (IMS-MS). IMS-MS obtains both structural and mass information in a single assay. It has demonstrated agreement in DAR characterization between IMS-MS and MS. Therefore, IMS-MS can be used to study the drug load distributions of ADCs and characterize mAbs.
However, the conventional methods for quantification and identification of drug conjugates have the drawbacks of arbitrary, low sensitivity, insufficient selectivity and/or relative long analysis time or high costs. For example, native MS, chromatographic, and IMS-MS approaches provide little information on site-specific drug conjugation of the mAb and whether the occupancy and location of drugs bound to the antibody alters the selectivity or efficacy of the ADC. Identification of the peptide with the complete drug molecule is challenging due to the size of the small molecule and the linker.
A ratio can be obtained to quantify conjugated peptides by normalizing the conjugated peptide intensity with the sum intensities of the unmodified peptide counterpart and the conjugated peptide. This Ad hoc ratio facilitates relative estimation of the conjugate-levels across samples for the site of interest. Stoichiometry-based approaches can also be used where the occupancy of a modification is indirectly determined by chemically removing the modifications or using a stable isotope labeled synthetic peptides and stable isotope labeled cell lines (See, e.g., Wu et al., Nature methods 8, 677-683 (2011); Lim et al., Journal of proteome research 16, 4217-4226 (2017)). Isobaric labeling specific to primary amines have been utilized to study occupancy of model NIST mAb to show the utility of a stoichiometry-based approach based on isobaric labeling (Hill et al., Sci Rep 8, 17680 (2018)). Nonetheless, stoichiometry-based quantification methods have not been integrated into the pharmaceutical pipeline for quantification or ADC analysis due to, for example, complex and challenging sample preparation, elaborate analytical schemes required to obtain compositional differences based on drug conjugation, etc. (Janin-Bussat et al., Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 981-982, 9-13 (2015); Le et al., Anal Chem 84, 7479-7486 (2012)).
An isobaric set of TMTs can be used to label and study a set of drug polypeptide conjugates, such as ADCs. Using high-resolution mass spectrometry, baseline resolution of these reporter ion masses and their isotopologues are possible for accurate relative quantitation. In one aspect of the application, TMTs are used in tandem mass spectrometry (MS2) analysis of a sample, particularly a drug conjugate, for the characterization of the sample based on a mass spec bar code of the sample.
A mass spectrum is a histogram acquired using a mass spectrometer. The mass spectrum of an analysis is usually plotted as intensity (Y) vs. m/z (mass-to-charge) ratio (X) plot. A reporter ion for a TMT can be detected by its m/z ratio on a mass spectrum. The Y-axis represents the signal intensity of the ions.
Depending on the instruments and software, the signal intensity can be measured and expressed differently. For example, when using counting detectors the intensity is often measured in counts per second (cps). When using analog detection electronics the intensity is typically measured in volts. In Fourier-transform ion cyclotron resonance mass spectrometry and Orbitraps, the frequency domain signal (the y-axis) is related to the power (˜amplitude squared) of the signal sine wave (often reduced to an rms power). Some software calculates reporter area rather than intensity. As used herein, with respect to a mass spectrum, the term “intensity” encompasses signal intensity of ions measured by any method and expressed in any format. For example, the “intensity” of a reporting ion can include reporter intensity, reporter area, or any other variations of signal intensity of ions from mass spectrometry analysis.
As used herein, a “mass spec bar code”, “MS bar code”, “reporter ions masses barcode,” “barcode” or “mass barcode” of a sample refers to a measurement of the sample from a tandem mass spectrometry (MS2) analysis that contains information of a set of reporter ions and the measurement is unique to the sample. A unique barcode for a sample can be based on the intensity and/or the presence (pattern) or absence of the set of TMT reporter ions detected from the tandem MS2. One or more barcodes can be used in the triple play analysis of the sample.
Accordingly, the present application provides an improved method for accurate, sensitive and efficient identification and quantification of drug conjugates. In one aspect of the invention, a drug polypeptide conjugate, such as an ADC, is sequential labeled with a first TMT, such as a Cys reactive IodoTMT, and a second TMT, such as a TMT reactive to the Lys or primary amine at the N-terminus, and the labeled drug conjugate is subject to tandem mass spectrometry analysis. The sequential labeling scheme helps interrogate drug conjugates. Two parallel sample preparation arms can be used to label the drug conjugate (conjugate sample) and unconjugated mock (reference or control sample) according to embodiments of the application. Both labels facilitate encoding of unique reporter ion signatures that distinguish the sample and mock or reference. The reference sample produces reference reporter ion channels and the conjugate sample produces sample reporter ion channels. Unique reporter ions are generated after dissociation by either HCD or via electron-based dissociation such as ETD. The number of channels in the reporter ion region, their ratios and/or other mass spec performing specifics associated with the TMTs can be used to create a unique barcode to characterize the drug conjugate. For example, the bar code can comprise a distinct set of reporter ion and/or mass barcodes.
As illustrated in
Another aspect of the application relates to a method of localizing the conjugation site on the polypeptide, e.g., by using one or two TMT reporter ions to trigger additional dissociation step to further characterize the peptide covalently linked to the drug. In one embodiment of the application, one or two TMT reporter ions are produced using a first tandem mass spectrometry analysis, such as an HCD analysis. The reporter ion(s) is then used to trigger an ETD scan of the peptide to obtain an ETD spectrum. The ETD spectrum complements the HCD spectrum for characterizing the conjugate such that ETD provides sequence ions that assist in the localization of the residue with the drug conjugate. Alternatively, the reporter ion(s) is then used to trigger an ECD scan to obtain an ETD spectrum for further analysis of the peptide.
In certain embodiments, an illustrated workflow has two parallel sample preparation arms, for the drug conjugate and unconjugated mock. For example, both IodoTMT6 and TMT6 labels facilitate encoding of unique reporter ion signatures that distinguish the sample and mock or reference, e.g., reference sample produce reference reporter ion channels and conjugate sample produces sample reporter ion channels. Also, the number of channels in the reporter ion region and their ratios encodes for occupancy and the type of experimental measurement that can be obtained, and each experimental measurement has a distinct set of reporter ions or mass barcodes.
Accordingly, an aspect of the application relates to a composition comprising a mixture of peptides labeled with at least one of a first TMT and a second TMT, optionally one or more unlabeled peptides, wherein the first TMT and the second TMT do not have the same reporter ion mass, and the mixture of peptides comprises at least one peptide that is conjugated to a drug and labeled with the at least one of the first TMT and the second TMT.
For example, a composition of the application can comprise a mixture of peptides derived from a conjugate sample labeled with at least one of a first TMT and a second TMT, optionally one or more unlabeled peptides. The composition can also comprise a mixture of peptides derived from a conjugate sample described herein and a mixture of peptides derived from a mock sample labeled with at least one of a third TMT and a fourth TMT, optionally one or more unlabeled peptides.
Referring to
Unlike peptides with a single cysteine, peptides with multiple cysteine residues can have drug covalently linked to one or more of the cysteine residues. For example, the two hinge cysteines of IgG1 and IgG4 mAbs have the possibility of double occupancy and single occupancy of any single cysteine. The four hinge cysteines on IgG2 (with IgG2-A, IgG2-B and IgG2-A/B isoforms) increase the possible combinatorial conjugation possibilities even further (Liu et al., MAbs 4, 17-23 (2012).
Methods according to embodiments of the application can be used to study a drug conjugate with one or more drugs conjugated to a polypeptide (such as an antibody). One or more drugs can be within a single digested peptide. The drug molecule(s) can be of the same type or different type.
Although, the sample preparation workflows remain the same for analyzing such conjugation reactions, the mass spectrometry parameters for TMT-triggering requires either 1 of 2 reporters or 2 of 2 reporters to be included in the trigger settings for localization of complete multiple conjugations or partial multiple conjugations respectively, respectively. Similar methods/schemes can be used to study drug conjugates containing more than three drugs using methods of the application in view of the present disclosure.
In the representative structure shown in
Any ADC, including but not limited to any of the ADCs illustrated in
In another embodiment, three different types of reporter barcodes are used to calculate the drug occupancy at each cysteine residue.
The dual-TMT workflow uses the normalization factor between the conjugate and mock samples to correct for deviations in occupancy estimates. For example,
In another embodiment, the sequence ions and immonium ions of corresponding drug fragments aid in determining site of occupancy of the conjugated peptide (see, e.g.,
Methods of the application can further include steps to improve the mass triggering, quantification sensitivity, and accuracy using methods known in the art in view of the present disclosure. For example, HCD-MS2 can be performed on a mass selected precursor ion of a TMT labeled peptide conjugate in an ion-routing multipole (see, e.g.,
Charge-loss ion generated by HCD-MS2, such as the reporter ion TMT-130, can be used to trigger ETD-MS2 and generate an ETD spectrum.
For example, co-isolation and co-fragmentation of peptides can result in loss of specificity of the reporting ion of TMT-130 that serves to trigger the ETD-MS2 analysis on the ADC-peptide to localize the conjugation site on the polypeptide. Higher trigger intensity thresholds or narrow isolation windows (e.g., 0.4 Da) can be used to improve the mass triggering (see, e.g.,
In another embodiment, SPS-MS3 can be used to further improve specificity and accuracy of the detection and quantification (see, e.g.,
For example, synchronous precursor selection was used in reporter quantification of a study on the ADC of MSQC8 having the structure shown in
Preferably, the conjugate is completely labeled with both TMTs. The completion of the labeling can be measured by the intensity of the reporting ion for the TMT on the mass spectrum. Results shown in
Methods according to embodiments of the application can also be used to characterize or identify the structure of the drug conjugated to a polypeptide. As shown in
According to other embodiments of the application, a dual TMTs mass spectrometry analysis according to the invention can also be used for profiling reaction time course for a conjugation reaction (see, e.g.,
In further embodiments of the application, a dual TMTs mass spectrometry analysis according to the invention can be used in multiplex quantification of drug polypeptide conjugates to increase the throughput and reduce the costs.
In certain embodiments of the application, a multiplexed triple play analysis can be used to analyze multiple samples containing conjugates having more than one conjugation sites. For example, synthetic peptides having 1-3 cysteine residues that are conjugated with the fluorescent molecule DCAM-3 are analyzed using a method of the application.
An algorithm, such as that reported by Savisky et al., can be used to correct for ratio compression (Savisky et al., 2013, Journal of proteome research 12, 3586-3598), the entire content of which is incorporated herein by reference. SPS-MS3 routine available on current instrumentation can also ameliorate ratio compression (McAlister, et al., Anal Chem 86, 7150-7158 (2014). Nevertheless, a method of the application, without any correction, can provide an accurate estimation of low-level conjugation where TMT response is a linear function of conjugation. The detection of low-stoichiometries of conjugates is beneficial as most off-target conjugations is undesirable and can be monitored more accurately. The multiplex TMT ratios and or the inverse TMT ratios (site-occupancy) were compared with fluorescence-based yield estimates. The linear dynamic range for fluorescence-based measurement is high compared to dynamic range for occupancy of TMT reporters due to ratio compression. However, TMT reporters with high-intensity are also channels that have low-level conjugation that can be measured with less ratio compression effects and missing values (Lim et al., Journal of proteome research 16, 4217-4226 (2017)).
In another embodiment, a method of the application comprises using a robotic system. For example, a Triple Play workflow of the application can be implemented on AsayMap Bravo liquid handling robotic system where dual labeling and sample multiplexing with TMT reagents can increase the accuracy and precision of TMT based quantitation.
In one embodiment, a sample multiplexing scheme of the application can analyze up to four ADC samples using dual TMT labeling of the sample, such as MSQC8, at two different concentrations in duplicate.
Four MSQC8 drug conjugate mimics were prepared at two concentrations; the original sample and another at half the concentration by diluting with MSQC8 with MSQC4. Each of these samples was a sample duplicated in the dual TMT labeling.
In one embodiment of the application, the relative occupancies between any two reaction steps can be determined when a reaction proceeds via an intermediate step or where the reference material is unavailable (see, e.g.,
The invention will now be described in further detail with reference to the following specific, non-limiting examples. It should be appreciated by those of skill in the art that the techniques disclosed in the examples which follow represent techniques discovered by the inventor that function well in the practice of the invention, and thus can be considered to constitute preferred modes for its practice. However, those of skill in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific embodiments which are disclosed and still obtain a like or similar result without departing from the scope of the invention.
Four different ratios (1:1, 1:9, 9:1, and 1:3) of reduced and drug conjugated NIST monoclonal antibody ((mAb), Sigma Aldrich, Catalog No. 8671) (Sample #1) to non-reduced and IodoTMT™-tagged NIST mAb (Sample #2) were prepared. NIST mAb was conjugated by using either iodoacetamide (IAA) (Sigma Aldrich, Catalog No. 16125) or Biotin PEO Iodoacetamide (Sigma Aldrich, Catalog No. B2059). For Sample #1, 2 aliquots of 100 μg of NIST (50 μl of 2 mg/ml NIST) were prepared by combining 10 μl of stock (10 mg/ml) with 40 μl of a solution that comprised 8M guanidine hydrochloride (GuHCl) (Sigma Aldrich, Catalog No. G3272), and 4 mM ethylenediaminetetraacetic acid (EDTA) (Sigma Aldrich, Catalog No. 03620). The NIST aliquots were then reduced by the addition of 10 μl of 1M DTT (Sigma Aldrich, Catalog No. 1019777001) and incubated at 37° C. for 1 hour. Alternatively, TCEP can also be used as a reducing agent instead of DTT. While DTT is usually active at neutral-basic pH conditions, TCEP has a wide pH range and a stronger reducing agent.
NIST was conjugated with 1M IAA. 1M IAA was prepared by adding 300 μl of trimethyl ammonium bicarbonate (TEAB) (ThermoFisher, Part of TMT labeling kit) to an Eppendorf tube, which was vortexed and sonicated for 20 minutes to equilibrate TMT reagents to room temperature prior to use. Then 24 μl of 1M IAA was added to the reduced NIST samples, which were incubated in the dark at room temperature for 1 hour. Following the incubation period, 15 μl of 1M DTT was added to the samples to quench the conjugation reactions. The samples were desalted by combining them with a buffer exchange solution (8M GuHCl+4M EDTA) and placed through a Zebra™ spin desalting column with a molecular weight cut off of 7 kDa (ThermoFisher, Catalog No. 89882).
To conjugate the reduced NIST samples with Biotin PEO Iodoacetamide, the samples were buffer exchanged to sulfhydryl-free PBS (phosphate-buffer saline) at a pH of about 7.5. Immediately before use, a 20 mM stock solution of Biotin PEO Iodoacetamide was prepared (190 μl of PBS was added to 2 mg of Biotin PEO Iodoacetamide in an Eppendorf tube). The reduced NIST samples in PBS were combined with 5 μl of 20 mM Biotin PEO Iodoacetamide and mixed. The reactions were incubated on ice or at room temperature for 2 hours. Following the incubation period, the samples were desalted. The samples were placed into a buffer exchange solution (8M GuHCl+4M EDTA) and run through a Zebra™ spin desalting column with a molecular weight cut off of 7 kDa.
Sample #2 (IodoTMT™-tagged non-reduced NIST samples) was prepared by generating 3 aliquots of NIST (50 μl of 2 mg/ml NIST combined with 10 μl of stock (10 mg/ml)) and added to 40 μl of a solution (8M GuHCl+4 mM EDTA)). Each aliquot was combined with 50 ul of the solution that contained 8M GuHCl and 4 mM EDTA to offset the volume. To reduce the samples for subsequent iodoTMT labeling, 10 μl of DTT was added to each aliquot and the samples were incubated at 37° C. for 1 hour. The following four ratios of Sample #1 and Sample #2 were prepared: 1) 1:9 ratio (10 μl of reduced NIST:90 μl of non-reduced NIST), 2) 1:3 ratio (25 μl of reduced NIST:75 μl of non-reduced NIST), 3) 1:1 ratio (50 μl of reduced NIST:50 μl of non-reduced NIST), and 4) 9:1 ratio (90 μl of reduced NIST:10 μl of non-reduced NIST). Each mixed sample contained 100 μg of NIST and 2 μl of IodoTMT™-LabelA1 (10 μl methanol was added to 200 μg of IodoTMT™) was added to all of the samples. The samples were then incubated in the dark at 37° C. for 1 hour. To quench the labeling reaction, 4 μl of 0.5M DTT was added to each sample and incubated for an additional 15 minutes at 37° C. in the dark. The samples had their buffer exchanged with TEAB and the IodoTMT™-labeled proteins were digested with 4 μl of trypsin (1 mg/ml) for 4 hours to overnight at 37° C. The trypsin was quenched with 2 μl of 98% formic acid and 2 μl of TMT-LabelB1 (800 μg dissolved in 40 μl of anhydrous acetonitrile) was added to each of the 4 samples. The samples were incubated in the dark at 37° C. for 1 hour and the reactions were quenched by the addition of 8 μl of 5% hydroxylamine for 15 minutes at room temperature.
In addition to Sample #1 and Sample #2, a reference or mock sample, termed Sample #3, was prepared and tagged with IodoTMT™ tag. Sample #3 was prepared similar to the protocol used for Sample #2, except 4 instead of 3 samples were generated. Sample #3 was separately trypsinized and labeled with TMT-LabelB2. Sample #1 and Sample #2 each were mixed with 132 μl of Sample #3 at a volume ratio of 1:1, which resulted in TMT labeled proteins with unique isobaric reporter ion masses that formed four multiplexed samples for mass spectrometry. A maximum of four reaction mixtures can be multiplexed with dual-labeling with five unique reporter masses from IodoTMT™ and amine reactive TMT10plex™ reagents (ThermoFisher, Catalog No. 90110). Dual labeled four conjugated samples and a single mock/reference control sample were combined at a volume ratio of 1:1:1:1:1, which created a single multiplexed sample for liquid chromatography-mass spectrometry.
Conjugated mAbs were labeled with a dual TMT labeling protocol. MSQC8 (Sigma Aldrich mAb antibody-drug conjugate mimic) and MSQC4 (Sigma Aldrich mAb standard) were used as the conjugated sample and the reference or mock sample, respectively. The samples were tagged with distinct IodoTMT™ tags following the sample protocol used as described for tagging NIST mAB and separately trypsinized to create peptides. The resulting peptide mixtures from MSQC8 and MSQC4 were separately labeled with distinct amine reactive TMT tags following the same protocol used to label NIST mAb with TMT or IodoTMT™. Following TMT dual labeling, the samples were mixed at a volume ratio of 1:1, which created a multiplexed single sample for mass spectrometry.
The dual TMT labeling protocol was implemented in an AssayMAP Bravo (Agilent) robotic system, which automates protein sample preparation prior to analysis with LC-MS. Dual TMT labeling of conjugation of unconjugated NIST mAb was performed as previously described. The In-Solution Digestion Single Plate Protocol was used according to the manufacturer's instructions, which is incorporated herein by reference in its entirety (see, worldwide web: agilent.com/cs/library/applications/application-protease-digestion-in-solution-assaymap-5994-1682en-agilent.pdf). The automated sample preparation was programmed to dispense a minimum volume of 5 μl and the samples were desalted with reverse phase protein clean up using RP-W cartridge application as known in the art. The different sample ratios (e.g., 1:1, 1:9, 9:1, and 1:3) were performed with the reformatting utility in the AssayMAP Bravo robotic system.
Synthetic mAbs with 1-3 cysteine residues were separately conjugated with N-(7-Dimethylamino-4-Methylcoumarin-3-yl)Maleimide (DACM-3; ThermoFisher, Catalog No. D10251) via an adapted manufacturers' protocol for protein labeling. A stock solution of 16.76 mM DACM-3 was prepared. 1 mg of each peptide was dissolved in 1 mL of a solution that comprised 100 mM PBS, 0.1M NaCl, 10 mM EDTA (pH 8.0) and 50 μl of 16.76 mM DACM-3. The peptides were sealed with a light protective cover and incubated for approximately 5 minutes at ambient temperature. Mass spectrometry was used to confirm that the peptides were completely conjugated. The relative fluorescence (relative fluorescent units or RFUs) of the samples and of a standard curve of N-acetyl-L-Cysteine (cysteine peptides standards from NIST monoclonal antibody were synthesized to 99.99% purity by Biomatik Corporation) was measured (Excitation=385 nm, Emission=465 nm, 455 nm cutoff) with a fluorescent plate reader (Spectramax M5, Molecular Devices). The relative concentration of free-thiol or conjugated thiol for each peptide was calculated from a regression analysis of the internal N-acetyl-L-Cysteine curve. N-acetyl-L-cysteine was determined to be essentially 100% reactive with DACM-3.
Following DACM-3 conjugation of the peptides, the peptides were labeled with TMT. Each DACM-3 conjugated peptide was mixed with an unlabeled peptide counterpart to create five mixtures at various stoichiometric ratios (0, 0.2, 0.4, 0.6 and 0.8). The unlabeled peptide served as a control. The samples were evaluated with a fluorescence assay as known in the art in view of the present disclosure to ensure that the DACM-3 label worked appropriately prior to TMT labeling. Amine reactive 6-plex TMT labeling was performed on each sample as previously described for TMT labeling of tryptic peptides. Finally, the labeled five mixtures and control were combined each at a volume ratio of 1:1 and analyzed with LC-MS using method known known in the art in view of the present disclosure.
Samples that were analyzed with LC-MS2 were separated on an Agilent Infinity 12900 UHPLC with an AdvanceBio Peptide Mapping column (Agilent, Catalog No. 864600-911) at 65° C. A 50 minute liquid chromatography (LC) gradient program that used LC-MS grade water with 0.1% formic acid as mobile phase A and acetonitrile as mobile phase B was conducted according to the following protocol: 0 min, 2% B; 35 min, 30% B; 40 min, 80% B; 45 min, 85% B; 45.5 min, 2% B; and a subsequent re-equilibration for 5 min at 2% B. The flow rate was set to 0.2 mL/min and the injection volume was 2 μl. The mass spectrometer was operated in positive ionization mode with a data dependent (dd) MS2 HCD (MS/MS-HCD) and electron transfer dissociation (ETD) methods as known in the art. The following interface conditions were used: emitter voltage, +2600 V; vaporizer temperature, 325° C.; ion transfer tube, 325° C.; sheath gas, 55 (arb (arbitrary unit)); aux gas, 10 (arb); and sweep gas, 1 (arb).
The following internal mass spectrometer settings were used for MS scans: RF (radio frequency) lens, 60%; AGC (automatic gain control) target, 1e6; maximum injection time, 50 ms; and 1 μscan in profile mode at 70K resolution on the Orbitrap (OT) mass analyzer. The method sequentially included a series of filters prior to any MS2 HCD events as known in the art. A monoisotopic peak selection filter was included and set as peptide for all methods and an intensity filter of 1e5 was used. Some methods used an optional charge state filter to select precursor charge states 2-6. Additionally, certain methods used an optional dynamic exclusion (DE) filter to more efficiently identify peptides in the samples with either a 12 s or 3 s exclusion window and exhibited the following common parameters: exclude n=1 times; +/−3 ppm; exclude isotopes; and single charge state per precursor. One method involved Apex detection, which used the following parameters: expected peak width, 6 s; desired apex window, 30%.
Five ddMS2 OT-HCD (Data Dependent MS/MS-Orbitrap Detection-Higher Energy Collision Dissociation) scans were performed with the following settings: quadrupole isolation, 1.6 m/z isolation window; detector type, Orbitrap, auto m/z normal scan range, 70 K resolution, 100 m/z first mass; AGC Target, 2e5, inject ions for all available parallelizable time, 50 ms maximum injection time; 1 μscan, profile. Following ddMS2 OT-HCD, a targeted mass trigger (TMT) was implemented, which is a scan that is only triggered if the system detects a product ion from the user defined list. Targeted ion masses included TMT reporter ion-specific to detecting a payload from the list of reporter masses (e.g., 126 to 131 Da) and only ions within the top 10 most intense mass-to-charge ratios were used for all mass triggers. The following conditions were used for ddMS2 OT-ETD (Data Dependent MS/MS-Orbitrap Detection-Electron Transfer Dissociation): MSn Level, 2; quadrupole isolation, 1.6 m/z isolation window; ETD reaction time of 50 ms; detector type, Orbitrap, auto m/z normal scan range, 30 K resolution; AGC Target, 5e4, inject ions for all available parallelizable time, 22 ms maximum injection time; 1 μscan, profile. The number of dependent scans between ddMS2 OT-HCD and ddMS2 OT-ETD was set to 1.
Data from the various mass spectrometry analyses were processed with Xcalibur™ Data Acquisition and Interpretation Software (ThermoFisher, Catalog No. OPTON-3096 7, MaxQuant and Perseus source proteomics data analysis software (MaxPlanck Institute), and R 3.6 statistical programming software (R Foundation for Statistical Computing, Vienna, Austria). The site occupancy was estimated by Equation 1 with IodoTMT reporter ions peak areas where A2 is the area of unconjugated sample and A1 is the area of conjugated sample. A normalization factor was calculated by Equation 2 using TMT reporter ions where B1 is the peak area of a conjugated sample and B2 is the peak area of an unconjugated sample:
It is understood that the examples and embodiments described herein are for illustrative purposes only, and that changes could be made to the embodiments described above without departing from the broad inventive concept thereof. It is understood, therefore, that this invention is not limited to the particular embodiments disclosed, but it is intended to cover modifications within the spirit and scope of the invention as defined by the appended claims.
The present application claims the benefit of priority to U.S. provisional application No. 62/902,958, filed on Sep. 19, 2019, the disclosure of which is incorporated herein by reference in its entirety.
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/US2020/051686 | 9/19/2020 | WO |
| Number | Date | Country | |
|---|---|---|---|
| 62902958 | Sep 2019 | US |
