Claims
- 1. A method for transferring a foreign polynucleotide carried by a viral vector to a recipient cell wherein said method comprises increasing the concentration of dNTP in the cytosol of said recipient cell at the time when said foreign poplynucleotide is effectively present in said cell by exposing the recipient cell to a dNTP delivery vehicle containing dNTPs wherein said vehicle is selected from the group consisting of virosomes, liposomes, and microvesicles.
- 2. The method, according to claim 1 wherein said delivery vehicle comprises a receptor for said viral vector such that said receptor becomes inserted into the membrane of said recipient cell as said dNTP is effectively delivered to the cytosol of said recipient cell.
- 3. The method, according to claim 1 wherein said delivery vehicle has, at its surface, molecules which target said delivery vehicle to said recipient cell.
- 4. The method, according to claim 1, wherein said recipient cell is a quiescent cell.
- 5. The method, according to claim 1, wherein said recipient cell is selected from the group consisting of hematopoietic stem cells, neurons, muscle cells, liver cells, and epithelial cells.
- 6. The method, according to claim 1, wherein said recipient cells are transformed ex vivo.
- 7. The method, according to claim 1, wherein said virus vector is replication deficient.
- 8. The method, according to claim 1, wherein said virus vector is a retrovirus vector.
Parent Case Info
This application claim benefit of U.S. provisional application Ser. No. 60,032,826 filed Dec. 12, 1996.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
5512295 |
Kornberg et al. |
Apr 1996 |
|
Non-Patent Literature Citations (2)
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Gao et al. Proc. natl. Acad. Sci. USA (1993) 90: 8925-8. Oct. 1993. |
Meyerhans et al. J. Virology, (1994) 68(1):535-40. Jan. 1994. |