Patent Application
20230125212
The invention generally pertains to means and methods for enhancing cannabinoids efficacy.
More specifically, the invention relates to invigorating performance of cannabinoid by means of combining compounds to prime the binding cannabinoid receptors and promoting demethylation processes.
Cannabinoid receptors, the molecular targets of the cannabis-derived compounds, are present throughout the body and are normally bound by a family of endogenous lipids—the endocannabinoids. Release of endocannabinoids is stimulated in a receptor-dependent manner by neurotransmitters and requires the enzymatic cleavage of phospholipid precursors present in the membranes of neurons and other cells. Once released, the endocannabinoids activate cannabinoid receptors on nearby cells and are rapidly inactivated by transport and subsequent enzymatic hydrolysis. These compounds might act near their site of synthesis to serve a variety of regulatory functions, some of which are now beginning to be understood. Recent advances in the biochemistry and pharmacology of the endocannabinoid system are reveling the opportunities that this system offers for the development of novel therapeutic agents.
The most studied and established roles for cannabinoid therapies include pain, chemotherapy-induced nausea and vomiting, and anorexia. Moreover, given their breadth of activity, cannabinoids could be used to concurrently optimize the management of multiple symptoms, thereby reducing overall polypharmacy. The use of cannabinoid therapies could be effective in improving quality of life and possibly modifying malignancy by virtue of direct effects and in improving compliance or adherence with disease-modulating treatments such as chemotherapy and radiation therapy. There is an urgent unmet clinical need for the use of cannabis or cannabis-derived compounds, in a range of disorders that can impair or destroy quality of life and are led by urgent unmet clinical need.
Furthermore, there is additionally an unmet need for enhancing the efficacy of cannabinoids.
It is one object of the invention to disclose a plant-based composition configured to enhance a physiological effect of a cannabis-derived compound, wherein said composition comprises a synergic combination of said cannabis-derived compound, at least one portion of at least one plant comprising a methylating/demethylating active agent, and at least one portion of at least one enhancer plant.
It is one object of the invention to disclose a plant-based composition configured to enhance a physiological effect of a cannabis-derived compound in the treatment of seizures in epilepsy, wherein said composition comprises a synergistic combination of said cannabis-derived compound, at least one portion of at least one plant comprising a methylating/demethylating active agent, and at least one portion of at least one enhancer plant,
wherein said cannabis-derived compound is CBD;
said at least one portion of at least one plant comprising a methylating/demethylating active agent is Hibiscus tiliaceus Linn comprising Dimethylglycine; and
said at least one portion of at least one enhancer plant, is selected from a group consisting of mango, myrcene, palmitoylethanolamide, fenugreek and any combination thereof.
It is one object of the invention to disclose a plant-based composition configured to enhance a physiological effect of a cannabis-derived compound in the treatment of lung cancer, wherein said composition comprises a synergistic combination of said cannabis-derived compound, at least one portion of at least one plant comprising a methylating/demethylating active agent, and at least one portion of at least one enhancer plant,
wherein said cannabis-derived compound is CBD;
said at least one portion of at least one plant comprising a methylating/demethylating active agent is olive, comprising Hydroxytyrosol; and
said at least one portion of at least one enhancer plant, is selected from a group consisting of mango, myrcene, palmitoylethanolamide, fenugreek and any combination thereof.
It is another object of the invention to disclose a composition, as defined in any of the above wherein said physiological effect is selected from a group consisting of increased bioavailability, increased appetite, amelioration of a disease condition, and any combination thereof, said disease condition is selected from a group consisting of nausea post chemotherapy, vomiting post chemotherapy, multiple sclerosis, nerve injury, ocular pain, headache, ADHD, anxiety, insomnia, convulsions, multiple sclerosis, cerebral ischemia, Parkinson's disease, epilepsy, osteoarthritis, psoriasis, systemic lupus erythematosus, diabetes, glomerulonephritis, renal ischemia, nephritis, hepatitis, vasculitis, myocardial infarction, inflammatory bowel disease (IBD), colitis, emesis, Crohn's disease, immune-related disorders, pain-related disorders, and any combination thereof.
It is another object of the invention to disclose a composition, as defined in any of the above, wherein said composition is further configured to target said physiological effect specific body parts or physiological systems.
It is another object of the invention to disclose a composition, as defined in any of the above, wherein said enhancer plant is selected from a group consisting of Maticaria chamimilla, Subgenus Pinus, Lavandula officinalis, Piper nigrum, Mangifera, Fenugreek, Citrus limon and any combination thereof.
It is another object of the invention to disclose a composition, as defined in any of the above, wherein said demethylating-methylating plant is selected from a group consisting of: Medicago sativa, Aloe succotrina, Anabasis alexandri, Hordeum Spontaneum, Hordeum spontaneum, Pimpinella Anisum, Mercurialis annua, Malus sylvestris, Cynara Scolymus, Mandragora autumnalis, Cynodon Dactylon, Citrullkus colocynthis, Nigella Sativa, Solaqnum nigrum, Eucalyptus camaldulensis, Theobroma Cacao, Quercus calliprinos, Oxalis pes-caprae, Carum carvi, Elettaria cardamomum, Daucus Carota, Sinapis Arvensis, Prunus (cerasus) avium, Allium schoenoprasum, Ziziphus spina-christi, Citrus medica, Kaolin, Eugenia caryophyllata, Caffea Arabica, Foeniculum vulgare, Glycyrrhiza glabra, Myrtus communis, Ruta chalepensis, Salvia officinalis, Valeriana officinalis, Coriandrum sativum, Papaver umbonatum, Zea mays, Chrysanthemum coronarium, Cucumis sativus, Cuminum cyminum, Phoenix dactylifera, Origanum dayi, Cichorium pumilum Rubia tenuifolia, Echinacea purpurea, Sambucus nigra, Lycium europaeum, Oenothera drummondii, Digitalis purpurea, Lvandula stoechas, Hibiscus, sea hibiscus, beach hibiscus, coastal (or coast) hibiscus, coastal (or coast) cottonwood, green cottonwood, native hibiscus, native rosella, cottonwood hibiscus Hibiscus tiliaceus Linn, Arisarum vulgare, Cupressus sempervirens, Lepidium sativum, Portulaca oleracea, Allium sativum, Equisetum ramosissimum Zingiber officinale Matricaria aurea, Vitis vinifera Linum pubescens Thymelaea hirsute, Vicia hybrida Lawsonia alba Rubus sanguineus Silybum marianum Mentha longifolia, Polygonum equistiforme Opuntia ficus indica, Corchorus olitorius Artemisia judaica Catha edulis Lavandula officinalis, Achillea fragrantissima Citrus limon Lens esculenta Inula viscosa, Vitex agnus-castus Leontice leontopetalum Ceratonia silique, Desmostachya bipinnata, Calendula officinalis Althaea officinalis Melissa officialil L. Achilloea millefolium Viscum album Teucrium capitatum, Paronichia argentea, Cyperus rotundus Myristica fragrans Olea europaea, Allium cepa Origanum vulgare, Plantago ovata Pistacia palaestina Petroselinum crispum Mentha pulegium, Mentha piperita Cyclamen persicum Rosa phoenicia Ananas comosus, Rumex cyprius, Punica granatum Solanum tuberosum Asparagus aphyllus, Cucurbita Trifolium purpureum Paphanus sativus Urtica plulifera Rosemarinus officinalis, Smilax aspera, Ephedra foemina, Juncus acutus, Chiliadenus iphionodes, Capsella bursa pastoris, Capsella bursa pastoris, Atriplex halimus Thymbra spicata Crataegus aronia Sarcopoterium spinosum, Ecballium elaterium Rhus coriaria Satureja thymbra Laurus nobilis Majorana syriaca, Notobasis syriaca, Quercus ithaburensis, Asphodelus ramosus Tamarindus indica, Camellia sinensis Capparis spinosa Salvia fruticosa Thymus vulgaris, Nicotiana tabacum Lycopersicum esclentum Ammi visnaga, Nicotiana glauca Hypericum triquetrifolium Curcuma longa, Brassica oleracea, Clematis cirrhosa Eminium spiculatum Juglans regia, Haplophyllum tuberculatum Mentha aquatica Nasturtium officinale, Marrubium vulgare, Artemisia sieberi Apium graveolens Matricaria recutita, Ficus carica, Lupinus pilosus, Avena sterilis, Withania somnifera, Malva sylvestris and any combination thereof.
It is another object of the invention to disclose a composition, as defined in any of the above, wherein said cannabis-derived compound is selected from a group consisting from a group consisting of cannabinoids, terpenes, phenolic compounds and any combination thereof.
It is another object of the invention to disclose a composition, as defined in any of the above, wherein cannabinoids are at least one of Tetra-hydro-cannabinoids (d9-THC), Tetra-hydro-cannabinoids (d8-THC), Tetra-hydro-cannabinolic acid (THCA-d9), Tetra-hydro-cannabivarin (THCV/THC-C3), Cannabidiol (CBD), Cannabidiolic acid (CBDA), Cannabidivarin (CBDV), Cannabigerol (CBG), Cannabigerolic acid (CBGA), Cannabinol (CBN), Cannabidiolic acid (CBNA), Cannabichromene (CBC), Cannabichromenic acid (CBCA), and any combination thereof.
It is another object of the invention to disclose a composition, as defined in any of the above, wherein terpenes are mono-terpenes or sesqui-terpenes.
It is another object of the invention to disclose a composition, as defined in any of the above wherein phenolic compounds are at least one of O-glycoside Cannaflavin A, Cannaflavin B, Canabisin D, and any combination thereof.
It is another object of the invention to disclose a composition, as defined in any of the above, wherein said composition is configured to be administrable in a manner selected from a group consisting of an inhaler, a cigarette, a tablet, a capsule, a pill, lyophilized, powder, emulsion, granulated powder, cream, ointment, paste, lotion gel, liquid, a solution, a patch and any combination thereof.
It is another object of the invention to disclose a composition, as defined in any of the above, wherein said composition is configured to be administrable in a manner selected from a group consisting of fast release, slow release, sustained release, controlled release and any combination thereof.
It is another object of the invention to disclose a composition, as defined in any of the above, wherein said composition additionally comprising ingredients selected from a group consisting solubilizers, stabilizers, buffers, tonicity modifiers, bulking agents, viscosity enhancers/reducers, surfactants, chelating agents, adjuvants and any combination thereof.
It is one object of the invention to disclose a method of enhancing a physiological effect of a cannabis-derived compound, comprising the steps of:
It is another object of the invention to disclose a method, as defined in any of the above, wherein said physiological effect is selected from a group consisting of increased bioavailability, increased appetite, amelioration of a disease condition, and any combination thereof, said disease condition is selected from a group consisting of nausea post chemotherapy, vomiting post chemotherapy, multiple sclerosis, nerve injury, ocular pain, headache, anxiety, insomnia, convulsions, multiple sclerosis, cerebral ischemia Parkinson's disease, epilepsy, osteoarthritis, psoriasis, systemic lupus erythematosus, diabetes, glomerulonephritis, renal ischemia, nephritis, hepatitis, vasculitis, myocardial infarction, cerebral ischemia, inflammatory bowel disease (IBD), colitis, emesis, Crohn's disease, immune-related disorders, pain-related disorders, and any combination thereof.
The following description is provided, alongside all chapters of the present invention, so as to enable any person skilled in the art to make use of said invention and sets forth the best modes contemplated by the inventor of carrying out this invention. Various modifications, however, is adapted to remain apparent to those skilled in the art, since the generic principles of the present invention have been defined specifically to provide means and methods for enhancing cannabinoids efficacy.
As used herein after, the term “methylation” refers herein after to the addition of a methyl group on a substrate, or the substitution of an atom (or group) by a methyl group. Methylation is a form of alkylation, with a methyl group, rather than a larger carbon chain, replacing a hydrogen atom.
These terms are commonly used in chemistry, biochemistry, soil science, and the biological sciences.
Active DNA demethylation regulates many vital biological processes, including early development and locus-specific gene expression in plants and animals.
In biological systems, methylation is catalyzed by enzymes; such methylation can be involved in modification of heavy metals, regulation of gene expression, regulation of protein function, and RNA processing. In vitro methylation of tissue samples is also one method for reducing certain histological staining artifacts. The counterpart of methylation is called “demethylation”.
In biological systems, methylation is accomplished by enzymes; methylation can modify heavy metals, regulate gene expression, RNA processing and protein function. The Methylation cycle in medicine relates to the metabolism of various systems including the production of glutathione.
As used herein after, the term “cannabis-derived compounds” refers hereinafter to compounds found in cannabis plant, comprising cannabinoids, terpenes and phenolic compounds. As used herein after, the term “cannabinoids” refers hereinafter to a class of diverse chemical compounds which are ligands for cannabinoid receptors in cells that alter neurotransmitter release in the brain. Cannabinoids were primarily discovered in marijuana (cannabis flower) and hashish (compressed cannabis resin) from the plant of Cannabis saliva. This plant contains more than 80 phyto-cannabinoids. The main active constituent of marijuana is the psychoactive Δ9-tetrahydrocannabinol (Δ9-THC), which acts at cannabinoid 1 (CB1) and cannabinoid 2 (CB2) receptors as a partial agonist. Other important natural cannabinoids present in marijuana are the non-psychoactive cannabidiol (CBD), Δ9-tetrahydro-cannabivarin (Δ9-THCV) and cannabichromene (CBC) [1-3]. Among them CBD has attracted the greatest attention thus far. It was shown to antagonize the effects of CB1/CB2 receptor agonists, to counteract the psychotropic and other negative effects of Δ9-THC and several data suggest that it behaves as an inverse agonist of CB1 and CB2 receptors. Some of these plant-derived cannabinoids are used in the medical practice, such as Δ9-THC (dronabinol) and its synthetic analogue, nabilone against chemotherapy-induced nausea and emesis, and as appetite stimulants (e.g. in AIDS patients). CBD combined with Δ9-THC (nabiximols) is used to relief neuropathic pain and spasticity in multiple sclerosis, and as an adjunctive analgesic treatment in advanced cancer pain.
As used herein after, the term “terpenes”, refers hereinafter to a large and diverse class of organic compounds, produced by a variety of plants. Terpenes are derived biosynthetically from units of isoprene, which has the molecular formula C5H8. The basic molecular formula of terpenes are multiples of that, (CsHs)n where n is the number of linked isoprene units. Terpenes are fragrant oils that give cannabis its aromatic diversity.
As used herein after, the term Cannabidiol (CBD), refers to Cannabis sativa constituent, which is a pharmacologically broad-spectrum drug that in recent years has drawn increasing interest as a treatment for a range of disorders.
The cannabis derived compounds comprise:
As used herein after, the term “plant portion” refers hereinafter to several structures, found in plants. The plant or plant portions useful in the means and methods of the present invention include but no limited to intact plants, roots, tubers, berries, rhizomes, stems, leaves, flowers, shoots, fruits, grains, or seeds.
The term demethylating agents refers to a group of chemotherapeutic agents with the capacity, both in vitro and in vivo, to induce transient DNA hypomethylation. DNA methylation refers to the addition of a methyl group to a CpG site1. These sites cluster together in areas known as CpG islands and are frequently localized in the proximity of key gene regulatory regions such as gene promoters. DNA methylation, both aberrant and physiologic, of these areas can result in gene silencing and in the equivalent of the physical inactivation, due to either mutations or deletions, of tumor suppressor genes2*. At the present time, two hypomethylating agents are approved in the U.S. and are widely used in Europe and the rest of the world: 5-azacitidine3 and 5-aza-2′-deoxycitidine4 (decitabine). These two agents have significant activity in patients with higher risk myelodysplastic syndromes (MDS). Recently, 5-azacitidine has been reported to improve survival in patients with higher risk MDS in a randomized phase III study.
Plants comprising methylating/demethylating active agents, are used primarily to increase efficacy of administered cannabinoids or cannabis-based compounds and to target the effect of administered cannabinoids to various body parts or physiological systems, such as nervous system, immune modulation, analgesia (such as pain relief, chronic pain, neuropathic pain), arthritis, muscles, skin, respiratory system, cardiovascular system, gastrointestinal tract, body organs, tumors, metastases etc.
Furthermore, the methylating/demethylating agents exert their activities in various body parts, or physiological systems of the treated mamma, thereby targeting their effect to these body parts or physiological systems.
Table 1 discloses the list of plants comprising methylating and demethylating active agents.
Medicago sativa
Aloe succotrina
Anabasis alexandri
Hordeum Spontaneum
Hordeum spontaneum
Pimpinella Anisum
Mercurialis annua
Malus sylvestris
Cynara Scolymus
Mandragora autumnalis
Cynodon Dactylon
Citrullkus colocynthis
Nigella Sativa
Solaqnum nigrum
Eucalyptus camaldulensis
Theobroma Cacao
Quercus calliprinos
Oxalis pes-caprae
Carum carvi
Elettaria cardamomum
Daucus Carota
Sinapis Arvensis
Prunus (cerasus) avium
Allium schoenoprasum
Ziziphus spina-christi
Citrus medica
Kaolin
Eugenia caryophyllata
Caffea arabica
Foeniculum vulgare
Glycyrrhiza glabra
Myrtus communis
Ruta chalepensis
Salvia officinalis
Valeriana officinalis
Coriandrum sativum
Papaver umbonatum
Zea mays
Chrysanthemum coronarium
Cucumis sativus
Cuminum cyminum
Phoenix dactylifera
Origanum dayi
Cichorium pumilum
Rubia tenuifolia
Echinacea purpurea
Sambucus nigra
Lycium europaeum
Oenothera drummondii
Digitalis purpurea
Lvandula stoechas
Arisarum vulgare
Cupressus sempervirens
Lepidium sativum
Portulaca oleracea
Allium sativum
Equisetum ramosissimum
Zingiber officinale
Matricaria aurea
Vitis vinifera
Linum pubescens
Thymelaea hirsuta
Hibiscus tiliaceus Linn
Vicia hybrida
Cannabis sativa
Lawsonia alba
Rubus sanguineus
Silybum marianum
Mentha longifolia
Armoracia rusticana, syn.
Cochlearia armoracia
Polygonum equistiforme
Opuntia ficus indica
Corchorus olitorius
Artemisia judaica
Catha edulis
Lavandula officinalis
Achillea fragrantissima
Citrus limon
Lens esculenta
Inula viscosa
Vitex agnus-castus
Leontice leontopetalum
Ceratonia siliqua
Desmostachya bipinnata
Calendula officinalis
Althaea officinalis
Melissa officialil L.
Achilloea millefolium
Viscum album
Teucrium capitatum
Paronichia argentea
Sinapis alba
Brassica nigra
Brassica juncea
Cyperus rotundus
Myristica fragrans
Olea europaea
Allium cepa
Origanum vulgare
Plantago ovata
Pistacia palaestina
Petroselinum crispum
Mentha pulegium
Mentha piperita
Cyclamen persicum
Rosa phoenicia
Ananas comosus
Rumex cyprius
Punica granatum
Solanum tuberosum
Asparagus aphyllus
Cucurbita
Trifolium purpureum
Raphanus sativus
Raphanus sativus L. var.
niger J. Kern
Urtica plulifera
Rosemarinus officinalis
Smilax aspera
Ephedra foemina
Ephedra campilopoda
Juncus acutus
Chiliadenus iphionodes
Capsella bursa pastoris
Capsella bursa pastoris
Atriplex halimus
Thymbra spicata
Crataegus aronia
Sarcopoterium spinosum
Ecballium elaterium
Rhus coriaria
Satureja thymbra
Laurus nobilis
Majorana syriaca
Notobasis syriaca
Quercus ithaburensis
Asphodelus ramosus
Tamarindus indica
Camellia sinensis
Capparis spinosa
Salvia fruticosa
Thymus vulgaris
Nicotiana tabacum
Lycopersicum esclentum
Ammi visnaga
Nicotiana glauca
Hypericum triquetrifolium
Curcuma longa
Brassica oleracea
Clematis cirrhosa
Eminium spiculatum
Juglans regia
Haplophyllum tuberculatum
Mentha aquatica
Nasturtium officinale
Marrubium vulgare
Artemisia sieberi
Apium graveolens
Matricaria recutita
Ficus carica
Lupinus pilosus
Avena sterilis
Withania somnifera
Malva sylvestris
Enhancers: Enhancer plants are administered along with the methylating and demethylating plants.
Enhancer plants are primarily used to enhance bioavailability. The route of administration is an important determinant of the pharmacokinetics of the cannabinoids in cannabis, particularly absorption and metabolism. Typically, cannabis is smoked as a cigarette. The main advantage of smoking is rapid onset of effect and ease of dose titration. When cannabis is smoked, cannabinoids in the form of an aerosol in the inhaled smoke are absorbed and delivered to the brain rapidly, as would be expected of a highly lipid-soluble drug.
However, smoking is difficult to control, and is not easily standardized. Furthermore, Smoking anything, including cannabis, is not beneficial for the lungs and airway system. A healthier option may be vaporization; because cannabinoids are volatile, they will vaporize at a temperature much lower than actual combustion. Heated air can be drawn through cannabis, the active compounds will vaporize, and these can then be inhaled.
Vaporization delivers the substance in a rapid manner that, like smoking, can be easily titrated to the desired effect. This option removes most of the health hazards of smoking,
Cannabis can also be ingested orally or through a feeding tube. Thought to be safer than smoked cannabis in some ways (e.g., reduced carcinogen exposure), titration of dose through oral administration, unlike smoking, is difficult and may result in stronger and more frequent adverse side effects (e.g., panic, paranoia and performance impairment) This risk stems from slow and unpredictable absorption of orally administered cannabis. Orally ingested THC or cannabis has quite different pharmacokinetics than when it is inhaled. The onset of action is delayed and titration of dosing is more difficult.
Thus, there is a need for absorption enhancers. Non limited examples of plant-derived enhancers is listed in Table 2.
Maticaria chamimilla
Subgenus Pinus
Lavandula officinalis
Piper nigrum
Mangifera
Citrus limon
Glycine max
Trigonella foenum-
graecum
Euphorbia resinifera,
As used herein the terms sinusitis or rhinosinusitis, refer hereinafter to inflammation of the paranasal sinuses, the cavities that produce the mucus necessary for the nasal passages to function effectively. Common symptoms include thick nasal mucus, a plugged nose, and facial pain. Other signs and symptoms may include fever, headaches, a poor sense of smell, sore throat, and a cough. Serious complications are rare. Acute sinusitis is defined if it lasts fewer than 4 weeks, and chronic sinusitis—if it lasts for more than 12 weeks. Sinusitis can be caused by infection, allergies, air pollution, or structural problems in the nose. Most cases are caused by a viral infection. A bacterial infection may be present if symptoms last more than 10 days or if a person worsens after starting to improve.
As used herein the term “about” denotes ±25% of the defined amount or measure or value.
The current invention discloses a smoking (or inhaling) composition comprising Cannabidiol (CBD) (or any other cannabinoid) mixed with at least one of herbs/plants of methylating/demethylating plants and at least one of enhancer plants.
Methylating/demethylating plants are used primarily to increase efficacy of administered cannabinoids or cannabis based compounds.
Plants comprising methylating/demethylating active agents are used primarily to increase efficacy of administered cannabinoids or cannabis-based compounds and to target the effect of administered cannabinoids to various body parts or physiological systems, such as nervous system, muscles, skin, gastrointestinal tract immune modulation, pain relief, etc.
The composition is smoked by pre-rolled cigarettes, hand-rolled cigarettes or an herbal mixture for rolling or using an inhaler.
The current example discloses a composition which exerts synergistic effect related to absorption, targeting and physiological effects of cannabinoid or cannabis-related compounds. The relevant effects that may be affected by the current invention includes at least one of the aforementioned physiological effect:
Therapeutic indications for cannabis are assumed to target the endocannabinoid system, which has receptors (CB1 and CB2) involved in cognition, memory, analgesia, psychomotricity, appetite and immune function. There are, however, few data specifically on the efficacy of medicinal cannabinoids for the various indications suggested to date. There are even fewer data for a variety of specific populations such as pregnancy, young people, elderly individuals with complex comorbidities and those with mental health issues. Substantial reliance on research evidence from recreational use of cannabis is therefore required until robust data specifically for medicinal cannabinoids become available. A broad range of therapeutic effects of cannabinoids has been suggested and is currently the focus of much research. Delta-9-tetrahydrocannabinol (THC) has been proposed to be of benefit for chronic pain, nausea and vomiting induced by chemotherapy, and in the reduction of spasms in multiple sclerosis. Cannabidiol (CBD), the second most abundant constituent within cannabis, cannabidiol (CBD), is thought to have a broad range of therapeutic properties, including amelioration of the adverse psychological and cognitive effects of THC. CBD is a low-affinity CB1 and CB2 receptor ligand and negative allosteric modulator of CB1, which reduces the binding of CB1 agonists, while augmenting endocannabinoid tone in an indirect manner. Therapeutic effects of CBD include, but no limited to better cognitive performance, especially memory, and increased gray matter in the hippocampus; neuroprotection, specifically through increased hippocampal cell survival and neurogenesis. Cannabidiol (CBD) has additionally antipsychotic, anxiolytic and anticonvulsant properties and may reduce Parkinsonian symptoms.
The feeling of increased appetite following the use of cannabis has been documented for hundreds of years and is known colloquially as “the munchies” in the English-speaking world. Clinical studies and survey data have found that cannabis increases food enjoyment and interest in food.
In summary, the therapeutic areas currently best associated with exploitation of Cannabis-related medicines include pain, cancer epilepsy, feeding disorders, Parkinson's disease, and immune modulation.
Efficacy in Sinusitis or pain: The current example discloses a composition for amelioration or treatment of sinusitis or pain. The composition comprises a synergistic mixture or formulation of at least one cannabinoid and at least one of the followings plants: black radish (Raphanus sativus L. var. niger J. Kern); mustard (white/yellow mustard, Sinapis alba; brown mustard, Brassica juncea; or black mustard, Brassica nigra); horseradish (Armoracia rusticana, syn. Cochlearia armoracia); Ephedra (Ephedra campilopoda); tea plant (Camellia sinensis).
Efficacy of plant-based formulations in Epilepsy: Epilepsy is a serious brain disorder that could affect anyone at any age and could decrease life expectancy by about 18 years. There are many antiepileptic drugs available worldwide in the market, however around one third of those who develop epilepsy unfortunately continue to experience uncontrolled seizures. Despite the introduction of new antiepileptic drugs, the quality of life and therapeutic response for patients with epilepsy remains still poor. Clinical evidence seems to indicate that CBD is able to manage epilepsy both in adults and children affected by refractory seizures, with a favorable side effect profile. However, to date, clinical trials are both qualitatively and numerically limited, thus yet inconsistent. Therefore, further preclinical and clinical studies are undoubtedly needed to better evaluate the potential therapeutic profile of CBD in epilepsy, although the actually available data is promising.
Objective: The aim of the current study is to assess the effect of a new CBD formulation as seizures protection in the rat model of pilocarpine-induced epilepsy.
Formulation: The plant-based formulation of epilepsy treatment is composed of the following ingredients: (Tables 3A-B)
Hibiscus
tiliaceus Linn
Cannabis Sativa
Study variables and endpoints: The formulation is administered IP (Intraperitoneally). The animals are assessed for:
Administration of the Test Items Each Test Item and vehicle formulation are administered to tested animals, according to Table.
Administration: Administration of Formulations, Methyl Scopolamine and Pilocarpine is performed as follows:
Clinical Observations: The animals are observed for toxic/adverse symptoms at acclimation and before TI/vehicle/CBD administration and continuously for 120 minutes post pilocarpine administration.
Seizure scoring: Following model induction by Pilocarpine injection, the animals are video recorded for 2 hours. The videos are analyzed and seizures rating are recorded according to the following Racine scale below.
Results
The results clearly show significant synergistic efficacy of the Mid dose formulation, which contains only 12.5 mg/kg CBD, in reducing the seizure levels, as compared to vehicle group and to saline group (Student's T-test p-values of 0.02 and 0.05, respectively) and borderline significant efficacy as compared to the CBD (25 mg/kg) group (Student's T-test p value of 0.13).
The results are summarized in Table 6 and depicted in
Efficacy of plant-based formulations in Lung Cancer: The formulation for studying the efficacy in a lung cancer model is delivered by IP administration
The formulation ingredients are listed in Table 7A-B:
Olea europaea
Cannabis Sativa
Lung Cancer Model—Study Outline
Female nude mice, are inoculated orthotopically (Lung injection) with lung cancer cells (A549). At tumor detection, mice are randomized to enter the study.
Study Design: The mice are allocated to the treatment groups according to Table 8.
Treatment: Test items are administered 7 times a week starting Day 4 post inoculation and for at least 14 days.
Animals are examined for
Termination: is performed at day 14-21 or according to bodyweight loss and morbidity standards.
Followed by necropsy and Lung/Tumor excision, measurement and weight.
Maximum tolerated dose of two plant based formulations (CBD mixtures), following single ip administration to SD rats
The objective of the study was to evaluate the Maximum Tolerated Dosage (MTD) of two CBD mixtures following Intra Peritoneally (IP) administration to Sprague-Dawley (SD) rats.
Study Variables and Endpoints:
The goal of the current pre-clinical study is to find the Maximal Tolerated Dose (MTD) of two CBD based formulations: Epilepsy formulation and lung cancer (LC) formulation, following a single peritoneal administration to Sprague-Dawley (SD) rats, therapeutic intervention in Epilepsy and Lung Cancer. Dosing is performed in an escalating mode; each dose elevation was dependent on the outcome of the previous dose. According to the results which included body weight and clinical signs assessment, there were no variations in the Tested Items treated rats compared to Vehicle treated rats.
Gross pathology examination based on macroscopic evaluations revealed A-symmetric thymus, in Low-dose-treated rats and slightly enlarged intestine lymph nodes (Peyer's patches), in the Mid-dose-treated rats in both Test Items and Vehicle treated groups.
Since the results were detected in the Vehicle treated rats with no dose relation to the tested items, they were not considered to be related to the Test Items' treatments.
Thus, it can be concluded that a single peritoneal administration of the Test Items to female SD rats, at the highest dose of the 500 mg/kg—for Epilepsy formulation, and 50.0 mg/kg—for LC formulation, is well tolerated and did not cause any significant adverse clinical effects compared to vehicle treatment. This dose may be used as a starting dose for repeated dosing safety evaluation.
Materials and Methods:
Vehicle is composed of Alcohol (20%), Glycerin (5%), PC:PG (5%) Tween 20 (1%) and Distilled water (69%).
Formulation I—‘Epilepsy’ formulation, is composed of Dimethylglycine; CBD; Myrcene and Palmitoylethanolamide, all of which were dissolved in the Vehicle. The dosage of each ingredient in mg/kg is presented in Table 9.
Formulation II—‘Lung Cancer’, is composed of Hydroxytyrosol); CBD; Ephedrin; Myrcene and Palmitoylethanolamide, all of which are dissolved in the Vehicle. The dosage of each ingredient in mg/kg is presented in Table 9.
Results:
Morbidity and Mortality: No morbidity or mortality related to the Test Items was observed in all groups during the in-life period.
Body weight (BW) was monitored in all animals of all groups. As shown in Figure the average BW gain in the groups treated with both Can-Epilepsy formulation (
Detailed and Cage-side Clinical Observations: No clinical sign abnormalities were observed in rats treated with Vehicle group 1F); Can-Epilepsy (Epilepsy formulation) (groups 2F, 3F and 4F) and Can-LC (Lung cancer formulation) (groups 5F, 6F and 7F) during the study. Individual clinical observations are summarized in Table 10.
Gross Pathology: The rats were evaluated for macroscopic findings in Gross pathology. As shown in Table 5 during the Gross Pathology of Cycle I (Low dose), rats No. 1, 4, and 13 from groups 1F (Vehicle); 2F (Can-Epilepsy) and 5F (Can-LC) had an a-symmetric Thymus, which appeared also in Cycle II in rat No. 2 from the control group (F, Vehicle treated), but not in the treated ones in Cycle II nor in other rats from Cycle III. The tissues were preserved for possible future histology analysis.
Furthermore, in Cycle II (Mid dose), rats No. 8, 9, 16, from groups 3F (Can-Epilepsy) and 6F (Can-LC), had slightly enlarged intestine lymph nodes (Peyer's patches), which appeared also in the control rat No. 2 (group 1F, Vehicle) of Cycle II. These enlarged lymph nodes neither appeared in Cycle I nor III. The tissues were preserved for possible future histology analysis. Results are summarized in Table 11.
Conclusions: In this study a single peritoneal administration of the Test Items, Can-Epilepsy and Can-LC, were evaluated for maximal tolerated dose in female SD rats. BW and clinical signs evaluation demonstrated no variations in the Tested Items treated rats compared to Vehicle treated rats.
Gross pathology examination during termination day revealed A-symmetric thymus in Low-dose-treated rats from both Test Item groups, as well as in the Vehicle treated group. Furthermore, slightly enlarged intestine lymph nodes (Peyer's patches), were observed in the Mid-dose-treated rats from both Test Item groups as well as in the Vehicle treated group. Both findings were detected also in the control—Vehicle treated rats, therefore, they were not considered related to the Test Item treatment.
Based on the above findings and under the conditions of this study, it is concluded that a single peritoneal administration of the Test Items, Can-Epilepsy at dose levels of 125/250/500 mg/kg and Can-LC dose levels of 12.5/25.0/50.0 mg/kg in female SD rats, is well tolerated and does not cause any significant adverse clinical effects compared to Vehicle treatment. For future repeated-dosing safety studies, the highest single dose tested of the Can-Epilepsy and the Can-LC drugs (500 mg/kg and the 50.0 mg/kg respectively) can serve as a starting dose, since they revealed no Test Item related adverse effects under single peritoneal administration
Routes of administration: The combination of a cannabinoid and a methylation agent is formulated to either oral administration, intravenous administration, or topical administration.
The formulations of the present invention comprise inter alia, in a non-limiting matter, additional ingredients or pharmaceutical excipients to further develop a formula to have a desired concentration, effective doses, dosing regiments and treatment times. These ingredients include, inter alia, solubilizers, stabilizers, buffers, tonicity modifiers, bulking agents, viscosity enhancers/reducers, surfactants, chelating agents, and adjuvants.
Cannabis composition of the current invention is administered by smoking a plant-derived cigarette or by oral or intravenous administration. The smoked route is most commonly used is a ready premade-cigarette or hand-rolled cigarette.
Intravenous route offers precise control of dose and timing.
Cannabinoid composition is delivered also by or o-mucosal route.
Nasal administration (through the nose)—can be used for topically acting substances, as well as for insufflation of e.g. decongestant nasal sprays to be taken up along the respiratory tract. Such substances are also called inhalational, e.g. inhalational anesthetics.
Oral administration Oral drugs are taken as tablets or capsules.
Tablets: The dissolution of the tablet can be affected significantly by particle size and crystal form.
The dissolution time can be modified for a rapid effect (fast dissolution) or for sustained release, (slow dissolution rates which prolong the duration of action or avoid initial high plasma levels).
Capsules: A capsule is a gelatinous envelope enclosing the active substance. Capsules can be designed to remain intact for some hours after ingestion in order to delay absorption. They may also contain a mixture of slow- and fast-release particles to produce rapid and sustained absorption in the same dose.
Oral sustained release: Oral sustained release in capsules or tablets is achieved, in a non-limiting matter, by embedding the active ingredient in an insoluble porous matrix, such that the dissolving drug must make its way out of the matrix before it can be absorbed, sustained release formulations in which the matrix swells to form a gel through which the drug exits, or by an osmotic controlled-release oral delivery system, where the active compound is encased in a water-permeable membrane with a laser drilled hole at one end. As water passes through the membrane the drug is pushed out through the hole and into the digestive tract where it can be absorbed.
Solutions: Pharmaceutical solutions are extensively used as dosage forms for the oral administration of therapeutic agents. Pharmaceutical solutions defined as liquid preparations in which the therapeutic agent and the various excipients are dissolved in the chosen solvent system.
Pharmaceutical solutions are homogeneous, i.e. the therapeutic agent(s) and excipients are dissolved in the vehicle Parenteral administration: Parenteral administration is performed using intravenous, subcutaneous, intramuscular, and intra-articular administration. The drug is stored in liquid or if unstable, lyophilized form.
Topical administration: Topical formulations comprise inter alia cream, ointment, paste, lotion or gel.
Transdermal delivery: Transdermal delivery is achieved, for example, by transdermal patches.
Alternative routes of administration are suppository, intraventricular, intramuscular, inhalational, aerosol, and sublingual.
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/IL2021/050353 | 3/29/2021 | WO |
| Number | Date | Country | |
|---|---|---|---|
| 63001335 | Mar 2020 | US |
